LOCUS       DASUQP010000001         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1363880, whole genome shotgun sequence.
ACCESSION   DASUQP010000001 DASUQP010000000
VERSION     DASUQP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363880"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1707)
                     /locus_tag="VFY74_00005"
     CDS             complement(325..1707)
                     /locus_tag="VFY74_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX5930127.1"
                     /translation="MRTECNPEPLLFAPVDGRAVVASFDGGAITSDAGALLLGATDRA
                     IDLVGRFAACFSDGRDRERIAHAVATLVAQRVFGIALGYEDLIDHDQIRHDPVLAVLA
                     GKLAARRCGWAPLAGKSTLNRLEHAPSGGPGRYHKIGHDATAVERLFVDLFLEAHARA
                     PKQIILDLDATDDPVHGRQEGRFFHGYYDAYCYLPLYVFCGRHLLAAKLRRSNIDAST
                     GAAEEVARIVVQIRSRWPRVRILLRADSGFAREGLMTWCEANRVDFVFGLARNAHLVE
                     EIAVELVQAEREAGRTGQPARRYRDFRWATLDSWSRRRRVIGKAEWTGGEPNPRFVVT
                     SLKPGEVTARHLYEQIYCARGDMENRIKEAQGDLFADRTSAATLRANQLRLWFASMAY
                     VLICALRRIGLAHTQFAEATCGTIRLKLLKIGALVRASVRRIKIAMASAYPYQPEFAL
                     AHARLTAAVR"
     gene            complement(1803..>2248)
                     /locus_tag="VFY74_00010"
     CDS             complement(1803..>2248)
                     /locus_tag="VFY74_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5930128.1"
                     /translation="QRYPDCLMGMKDSSGDLAFASLVANEIKDFDVFPSAEGAIAHAR
                     DHGYAGCISATANITAPFARAAWIKPHLQEGREGLFKAVTLRKALSSSKLVASVKWAL
                     SRLHRDPSLTRVMPPLTSLGEMEVRTLEATLAQTAFSELAFGAPP"
BASE COUNT          369 a          722 c          775 g          382 t
ORIGIN      
        1 cgtgaggtcg ggcccgaggt atgtcacgcg cgtgccggtc cgtcgttacg tcacccgcac
       61 cgaggtcctg gctccggtgc cgcgctatgt gactcgggtc gtgaccacgg cccccgagat
      121 ccgtcgggcc cgcatcgtcg aacggcgggt ttactactag cccggatttc tcaccacgcg
      181 acgtgatcgg tggtggttcg agttctggat ccggcgctgt gggctacgtt ggggtgtgcg
      241 acctggcttg gagctgattt tgggcgcgtt gtcgccgccg cgctcgcggt ggggggtcta
      301 tggcgccgcg tttcccgggt cgtgtcacct caccgcggcg gtcaatcggg cgtgagcgag
      361 ggcgaactcg ggttggtacg ggtaggcgga ggccatggcg atcttgatcc gccgcacgga
      421 ggcgcgcacc agcgccccga tcttcaggag cttgagccgg atcgtgccgc aggtcgcctc
      481 ggcgaattgg gtgtgtgcga gaccgatccg tcgcaacgcg cagatcagga cgtaagccat
      541 cgaggcgaac cagagccgca gctggttggc ccgcagggtc gcagccgagg tgcggtcggc
      601 aaagagatcg ccctgcgctt ccttgatgcg gttctccatg tcgccccgag cgcagtagat
      661 ctgctcgtag aggtggcggg ccgtcacctc gccgggcttg agcgaggtca ccacgaagcg
      721 cgggttgggc tcgcctcctg tccattcggc cttgccgatc acgcgccgcc ggcgcgacca
      781 cgagtcgagc gtcgcccagc ggaagtccct gtagcgccgg gccggttggc cggtgcgccc
      841 ggcctcccgc tcggcttgaa cgagttcgac cgcgatctct tcgacgaggt gcgcgttgcg
      901 ggcgagcccg aacacgaagt cgacccggtt cgcttcgcac caggtcatca ggccctcgcg
      961 cgcgaagccc gagtcggccc gcagcaggat gcgaacccgc ggccagcgcg accggatctg
     1021 gaccacgatg cgtgcgacct cctcggccgc gcccgtactc gcatcgatgt tggagcggcg
     1081 cagcttggcg gcgaggagat gccggccgca gaagacgtag agcggcagat agcaatacgc
     1141 gtcgtagtag ccatggaaga agcgaccctc ctggcgcccg tggacgggat cgtccgtggc
     1201 gtcgaggtcc aggatgatct gcttgggcgc ccgggcgtgc gcctccagga agaggtccac
     1261 gaacaggcgc tcgaccgccg tcgcatcgtg gccgatcttg tgataccgcc cggggccgcc
     1321 agagggggcg tgctcaagtc ggttcagagt cgacttgccg gcgagcggcg cccacccgca
     1381 gcgcctggcc gcgagcttgc cggcgagcac cgccaacacg ggatcgtgcc ggatctgatc
     1441 atgatcgatg aggtcctcat agccgagcgc gatcccgaag acgcgctggg ccacgagcgt
     1501 cgccaccgcg tgcgcgatgc gctcgcgatc gcggccatcg ctgaagcagg ccgcaaagcg
     1561 cccgacgaga tcgatggccc gatcggtcgc gccgagcagc agcgcgccgg catcggaggt
     1621 gatcgctcca ccgtcgaacg aggccaccac ggcccgtccg tccacaggtg caaagaggag
     1681 cggttccggg ttacactccg ttcgcatcgg ggatctcctc tgagctcatc caagtcgttg
     1741 cgtcacaaca actttggatg attcagagcc ccgatgcacc ccaccctagt gagaaatctg
     1801 ggctaaggcg gcgcgccgaa tgcaagttcc gaaaatgccg tttgtgccag ggtagcctcg
     1861 agggttcgaa cctccatttc tcccagagat gtgagcggcg gcatgacgcg ggtcaaggac
     1921 gggtcacggt gaagccgcga gagggcccac ttcacgctag cgacaagctt cgagctgctc
     1981 agagctttcc gaagcgtgac ggccttaaat agaccttcgc ggccctcctg aaggtggggc
     2041 ttgatccacg ccgcgcgagc gaacggagcg gtgatgttgg ccgtcgcaga tatgcagccc
     2101 gcgtagccgt ggtcgcgggc gtgtgcaatg gcgccctctg cgctcggaaa cacatcaaag
     2161 tctttgattt cgtttgccac cagcgacgcg aaggcgagat cgcccgagga gtctttcatc
     2221 cccatcagac agtcgggata tcgttgct
//
LOCUS       DASUQP010000002         8215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_818905,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000002 DASUQP010000000
VERSION     DASUQP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818905"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFY74_00015"
     CDS             complement(<1..325)
                     /locus_tag="VFY74_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930129.1"
                     /translation="MTFALTPARLTLLVGLVLFGVAVPLLAPAYQAQATELLLFIVFA
                     LAWDLVGGQMGYNSFGNVLFVGLGMYVSAIVQVGLFYDVGLYTGAKGGGTEFVFDARQ
                     YLMGLA"
     gene            complement(337..1239)
                     /locus_tag="VFY74_00020"
     CDS             complement(337..1239)
                     /locus_tag="VFY74_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5930130.1"
                     /translation="MIANLQILFEAPALGLQLVIDGILIGAVFALAAYGMALVWGVTN
                     IVNVCQGEFVMLGGFVTVLLAEQGMPPLLAAPVAAAVLYALGLILFQAVIVHLIGRDL
                     FISLLATFGIAIVLQQLMARGFGAADRIVESGLGSWFLFDGLVGISKVKVLVFAACVL
                     LGAALTAFLSRSRQGQAIRATAQEPRAASVLGIDTRQVYRLAYALNAAICGAAGALAV
                     MAYTIHPYGGLSYTIRSFMIVVVAGIGNLFGIVWAAFGLGIAESFAGFIFGPQYQIAF
                     VFALLVVVLVLRNWRLKAQRRYLE"
     gene            complement(1250..2473)
                     /locus_tag="VFY74_00025"
     CDS             complement(1250..2473)
                     /locus_tag="VFY74_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5930131.1"
                     /translation="MWPARLLPAGRSAAVLLSLAASPALGADAEIVLGAAVSMTGKYA
                     QNGANTKNGYELAVRVINDKGGVEVGGTTYRIAVRYYDDESTPARGTELAERLIKQDG
                     VKFMLGPYGSGLTKAILPVVEKYKVPMIEGNGAARELFTKGFRYTFAVLTTSDQYLAP
                     AIALAAEHAGKLGKAKENIKVALVVENDPFSQDVRAGVLDDVERHDMMVVIDDQLPPE
                     LNDMSVTLTKIKALKPDVLVVSGHEKGALTAVTQIESLRVHVPILAMTHCDSAQIVEK
                     LGKAAEHTFCAQQWHRSLGYKDALFGTAEDFATRFEAAYSYPAPYQAAQSAAAVHVFA
                     DAFKRAQSLDPEKVRDAIAVTDLETFYGPVKFDAAGRNVAKPMVLTQVQGGRYVVVTP
                     AKWAAGQPVVPRPLR"
     gene            complement(2565..3545)
                     /locus_tag="VFY74_00030"
     CDS             complement(2565..3545)
                     /locus_tag="VFY74_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX5930132.1"
                     /translation="MRWRGQRESENVEDRRDEGGGFGFPFPGGGARFPSSGGGRGGGI
                     GIIGLLIILGLMFFFGVDPRVIMTGGSPGGGDATYPDIRLPQERPDTTNFPVPDEPQG
                     PPVRRPQTTSEDDLKQFVAVVLGDTEDVWRDLFARYGARYNDPKLVLFSGGVRSACGL
                     GMAQMGPFYCPIDEKVYIDLDFYEDLKNRFKAPGDFAQAYVIAHEIGHHVQNLLGIAE
                     QVEGLRRRLDETEANALQVRMELQADCFAGIWANRAEESRDIIEPGDIEEALRAASAI
                     GDDRIQRQTQGYVVPDAFTHGSSEQRVRWFTRGYESGKLEVCDTFNADEL"
     gene            complement(3901..7017)
                     /locus_tag="VFY74_00035"
     CDS             complement(3901..7017)
                     /locus_tag="VFY74_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX5930133.1"
                     /translation="MTLPELSIKRPVFATVMSLVLVLLGLVCYQRLSVREYPAIDPPV
                     VTVQTSYPGASAAIVESQVTQVLEDSLAGIESIDFITSISRQESSQITVIFKLDRNPD
                     YAAADVRDRVGRVRGRLPDEIDEPIIQKVEADAQPILYLAFYSDRHSELEITDYADRY
                     VKDQLQTLSGVAEVRLFGEREYAMRIWLDPERLAAYQLSPQDIELALRRQNVEVPSGR
                     IESMQREFTVLAETDLRTPEQFNALIVKEANGHLVRLDEVGHAELGALDERRIVRFNG
                     KPAVALGVVKQATANPLEVSEEVRAVLPRITQSLPAGMEVRIAHDRSIFIAESIKNVY
                     RTIGEAILLVVLIIFLFLRSVRATLIPLVTIPVSLIGAFALMYALDFSINTLTLLALV
                     LAIGLVVDDAIVMLENIYRHVEEGMPPVKAALLGSREISFAVVAMTITLAAVYAPIGF
                     MTGVTGRLFTEFAWTLAGAVIVSGFVALTLSPMMCSKLLRKGGAQNFASRLIERFLQG
                     LTAAYRALLKSALAARPLVLVVGLAVAGASYFLFTALKGELAPVEDQGNISVVFRGPE
                     GATIEYTDANAKKLEAVALAVPEADRVFAIAGNPTVSQGRVVLRLKPWEERARTQQEI
                     ARTIAPDIIAVPGVVAFPANPPSLGQSSRSKPVTFVIQTARPYDELEQMVQAFLDKVR
                     DYPGLVDVDSDLKLNAPQLDISVDREKAAATGVEIDTLGRTLETMLGGRQVTRFKQDG
                     EQYDVVVKIADIDRQNPDDLRRIYVRGRDGAMIPLSNLITIKETVAPKELNHFDQLRA
                     ATITAQLAPGYTLSEGLDFLAETAQEVLPITAKVEYAGESREFRTARSSLYLIFLLAL
                     AFIYLVLAAQFESFTDPFIIMLTVPLSITGALLALWWSGGTLNIYSQVGLVTLIGLIT
                     KHGILIVEFANQLRTKGMPLGEAVVEAAALRLRPILMTTGAMVLGAVPLAIATGAGAE
                     SRQDIGWVIVGGLLVGTLFTLFVIPVVYSYLSRRVFLEADHEGLQGADVQALPPPRET
                     GERPAPAE"
     gene            complement(7021..8070)
                     /locus_tag="VFY74_00040"
     CDS             complement(7021..8070)
                     /locus_tag="VFY74_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5930134.1"
                     /translation="MRRNSLARGGGFWLLAAAIGIAAAGAAAQDGKTEAVAVEAARVV
                     AAPLSEQVTAVGTVLSDESVTISAEIPGRIEEIHFEEGQPVEKGAPLFTLDDSVYRAQ
                     LADAEAKLKLAEQTHKRTSQLFSSKYATAQSADETASNLAVSTAAVELARVQLDKAHI
                     LAPFAGIVGLRGVSVGEYITAGQALVNLEAIDPVKADFRVPEKFLPAIRVGQTIRVKV
                     DAFPDDSFEGKVYAIDPQLDVSGRSLLVRAEVPNADKRLRPGLFARVTVLLELKEAAL
                     SVPEQAIVPQGDSQFVFKIVDGKVQLTKVVTGTRREGRVEIVEGLAAGDQVVTAGQLK
                     IRDGTPVSVVDAIGA"
BASE COUNT         1524 a         2594 c         2687 g         1410 t
ORIGIN      
        1 ccgcgagccc catcaggtac tgacgcgcgt cgaacacgaa ctccgtgccg ccgcccttcg
       61 ccccggtata gagcccgaca tcgtagaaga ggccgacctg gacgatcgcc gagacataca
      121 tgccgagccc gacgaacagc acattgccga aggagttgta gcccatctgg ccgccgacga
      181 ggtcccaggc cagcgcaaag acgatgaaaa gcagaagctc cgtcgcttgc gcctgatagg
      241 ctggagcgag aaggggtacg gcgacgccga agagaacgag tcccacgagg agcgtgagcc
      301 gtgcgggggt gagcgcgaag gtcatgccgc gaccgcttat tcaagatagc ggcgctgtgc
      361 cttgaggcgc caattacgga gcacgagcac gacgacgaga agcgcaaaga cgaaagcgat
      421 ctgatattgc gggccaaaga tgaagccggc gaagctctcg gcgatgccga ggccgaaagc
      481 cgcccagacg atcccgaaga gattgccgat ccccgccacg accacgatca tgaacgagcg
      541 gatggtgtag gagagcccgc catagggatg gatcgtgtag gccatgacgg cgagtgcgcc
      601 ggcggcaccg cagatggccg cgttcagcgc gtaagccagg cgatagacct ggcgcgtgtc
      661 gatgccgagc acggacgccg ctctcggctc ttgcgcggtc gcgcgtatgg cctggccctg
      721 gcgggagcgc gacaagaacg ccgtgagcgc ggcaccgagc aggacgcaag cggcaaatac
      781 caacaccttc actttcgata tcccgaccag accgtcgaac aggaaccacg acccgagccc
      841 gctctcgacg atgcggtcgg ccgcgccgaa gccgcgcgcc atcaattgct gcaagacgat
      901 ggcgatgccg aacgtcgcca gcagcgaaat gaacaggtcg cggccgatga ggtgcacaat
      961 caccgcctga aatagaatga ggccaagcgc gtagagcacc gcggcggcga ccggggcggc
     1021 aaggagcggc ggcatgccct gttcggcgag gagcacggta acaaaaccgc caagcatgac
     1081 gaattcgcct tggcagacat tgacgatgtt ggtcacgccc cagaccagtg ccatgccgta
     1141 tgcggcgagc gcgaacacgg cgccgatcag aatcccgtcg atgacgagct ggaggccgag
     1201 cgcgggcgcc tcgaacaaaa tctgcagatt ggcgatcatg tcgcccgtgc taacgcagag
     1261 ggcgcgggac gacaggctgg cctgccgccc acttggccgg cgtgaccacc acatatcggc
     1321 cgccctgaac ctgggtgaga accatgggct tggcgacgtt gcggccggcg gcgtcgaact
     1381 tgaccggacc gtaaaacgtc tcaaggtccg tcacggctat cgcatcgcgc accttctcag
     1441 ggtcgaggga ttgcgcgcgt ttgaaagcat cggcgaaaac gtggaccgcc gcggccgact
     1501 gggccgcctg ataaggcgcc gggtaggaat aggccgcctc gaaccttgtg gcgaaatcct
     1561 cggccgtgcc gaacagcgca tccttgtagc cgagcgagcg gtgccattgc tgggcgcaga
     1621 aagtgtgctc ggcggccttg ccaagctttt cgacgatctg cgccgagtcg caatgcgtca
     1681 tggcaaggat cggcacgtgg acacgcagag actcgatctg ggtcaccgcg gtgagagcgc
     1741 ccttctcgtg cccggagacg acgagcacat ccggcttcaa ggccttgatc ttggtcaggg
     1801 tcaccgacat gtcgttgagt tccggcggca gctgatcgtc gatcaccacc atcatgtcgt
     1861 ggcgctcgac atcgtccagc acgccggcgc gcacgtcttg cgaaaacgga tcgttctcca
     1921 cgacgagggc gaccttgata ttctccttgg cttttccgag cttgccggca tgctcggcgg
     1981 cgagcgcgat ggcgggggcg agatattggt cggaggtggt gagcacggcg aacgtgtagc
     2041 ggaagccctt ggtgaagagt tcccgcgccg ccccgttgcc ttcgatcatc gggaccttgt
     2101 atttctcgac gacgggcaga atcgccttgg tcagcccgga gccgtaaggc ccgagcatga
     2161 acttcacgcc gtcctgcttg atcaaccgct cggcaagctc ggtgccgcgc gccggcgtcg
     2221 actcgtcgtc gtaatagcgc accgcgatcc ggtaggtcgt gccgccgacc tcgaccccgc
     2281 ccttatcgtt gatgacgcgg accgcaagct cgtagccgtt cttggtgttg gcgccgttct
     2341 gcgcatattt gccggtcatc gagacggcgg cgccaaggac gatctcggcg tcggcgccaa
     2401 gcgccgggct tgcggccagg ctgagcaaaa cggcggccga ccggcccgcc ggcaacaatc
     2461 gcgctggcca cacgtaagcc ccctccgctg ctcgctgcgc gaatcctttt gacgccatag
     2521 cctacgccgc ccgcggcggc gggttcaatg ggacgcaatc gcgcctagag ctcgtcggcg
     2581 ttgaacgtat cgcacacttc gagcttgccg ctttcgtagc cgcgggtgaa ccagcgcacg
     2641 cgctgttccg acgagccatg cgtgaacgcg tcgggcacga catagccttg cgtctgcctc
     2701 tggatgcgat cgtcgccgat ggcgctcgcc gcgcggagcg cctcctcgat atcgcccggc
     2761 tcgatgatat ccctgctctc ctcggcccga ttggcccaga tcccggcgaa gcagtcggcc
     2821 tgcaactcca tacggacttg aagcgcgttg gcctcggtct cgtccaggcg gcggcgcaag
     2881 ccctcgacct gttcggcgat gccgagaagg ttctgtacgt ggtggccgat ctcatgcgcg
     2941 atgacatagg cctgggcaaa gtcgccgggc gccttgaacc gattcttcag gtcctcatag
     3001 aaatcgagat cgatataaac cttctcgtct atcggacagt agaaggggcc catctgcgcc
     3061 atgcctaagc cgcaagccga gcgcacgccg ccagagaaca gcacgagttt cggatcattg
     3121 tagcgggcac cgtatcgggc gaagaggtca cgccagacat cctcggtgtc gccgagaacg
     3181 actgcgacaa actgcttgag atcgtcttcg ctcgtggttt gagggcggcg caccggcggg
     3241 ccttgcggct cgtcaggcac ggggaagttg gttgtgtcag ggcgttcctg cggaaggcgg
     3301 atatcagggt acgtcgcatc gccgccgccc gggctgccgc ctgtcatgat gaccctcggg
     3361 tcgacgccga agaagaacat caagccgaga atgatcaaga gaccgataat tccgatgccg
     3421 ccgccccggc ctccgccgct gctcgggaag cgagcgccgc cccctgggaa cggaaagccg
     3481 aagccgccgc cctcgtctcg ccggtcttct acgttctcgc tctctctttg accacgccag
     3541 cgcatcttct caatcctcat accgggccaa gaatgccgcc caattttgcg cccattgtgc
     3601 atgcgccgtg tcgccgcaca aggccgggca ggaatggggg aactccgggg ccggtggcgc
     3661 gtcatccatt tcggcagtgt ttgagcccag tgcgcggtag gcgtgacggt acgtgagagt
     3721 gcgcccgcaa atccttgtgt tgatttggaa agactgacaa ggaaatgtca aaaaactgcc
     3781 acagggcgaa ggcatgctgc caatcgccca ggtgtgttta gtctgggtcg gtagtgcgtt
     3841 ctgtgtattg cgtacgagcc tcggcactcc ccgcgccggg gctcttcttt tttgcgcccg
     3901 ctactcggcg ggcgcgggtc tctcgcccgt ctcgcgcggc gggggaagcg cctgcacgtc
     3961 cgccccttgc aacccctcat gatcggcttc gaggaatacg cgccggctga gatatgaata
     4021 gaccaccggg atcacgaaca gggtgaagag cgtgccgacc aggaggccgc cgacgatcac
     4081 ccagccgatg tcctgtcggc tctcggcgcc cgcaccggtg gcgattgcga gcggcaccgc
     4141 accgaggacc atggcgccgg tcgtcatcag gatgggccgg agccggagcg ccgcggcctc
     4201 gacgaccgcc tcgccgagcg gcatcccctt tgttctcagc tgatttgcga actcgacgat
     4261 gaggatgccg tgcttggtga tcaatccaat caaggtcacg agtccgactt ggctatagat
     4321 gttcaatgtg ccgccgctcc accacagggc cagcagcgca ccggtgatcg acagcggcac
     4381 ggtcagcatg atgatgaaag ggtcggtgaa gctctcgaat tgcgcggcaa gcacaagata
     4441 gataaaggcg agcgcgagca ggaagatcag gtataggctc gagcgcgccg tcctgaattc
     4501 gcgcgactcg cccgcatact cgactttcgc ggtgatgggc agcacctcct gcgccgtctc
     4561 ggccaggaaa tccagcccct cgctcaaggt gtatcccggc gcgagctgcg cggtgatggt
     4621 ggcggcgcga agctgatcga agtggttgag ctctttcgga gcgaccgttt ccttgatggt
     4681 gatgaggttc gagagcggga tcatggcgcc gtcgcggccg cgcacataga tgcgccggag
     4741 atcgtcggga ttctgccggt cgatatcggc gatcttcacc accacgtcgt attgctcgcc
     4801 gtcctgcttg aatcgcgtga cctggcggcc gccgagcatg gtctcgagcg tgcggccgag
     4861 cgtgtcgatc tcgacgcccg tcgcggccgc cttctcgcgg tcgacggaaa tatcgagctg
     4921 cggcgcgttc agcttgaggt cgctgtcgac atcgacaagg ccggggtagt cgcggacctt
     4981 gtcgagaaag gcttggacca tttgttcgag ctcgtcgtag ggccgcgcgg tctgaatgac
     5041 gaaggtgacg ggtttcgacc gcgaactctg gccgagcgac ggcggattgg cgggaaaagc
     5101 gacgactcct ggaacggcaa tgatgtcggg ggcaatcgtg cgcgcaatct cttgctgggt
     5161 tcgggcgcgc tcttcccacg gcttgagccg cagaacgaca cggccttgcg acacggtcgg
     5221 gttcccggca atcgcgaaca cccgatcggc ctcgggcacg gccagcgcaa cggcttcgag
     5281 cttcttggcg tttgcgtcgg tgtattcgat ggtggcgccc tcggggccgc ggaatacgac
     5341 ggaaatgttc ccttggtcct cgaccggggc aagctcgcct ttgagcgccg taaacaagaa
     5401 gtagctcgct ccggccaccg ccagaccgac cacgagcacg agtgggcggg cggcgagcgc
     5461 gcttttcagg agagcccgat aggccgcggt cagtccttgc aggaagcgct cgatcaggcg
     5521 gctggcaaaa ttctgcgcgc cgcccttccg caacaatttc gagcacatca tcggcgaaag
     5581 cgtcaacgcc acgaagcccg acacgatgac ggcgccggcc aaggtccagg caaattcggt
     5641 gaacagccgc ccggtcacgc cggtcatgaa cccgatcggc gcatagacgg ccgcaagcgt
     5701 gatggtcatc gcaacgacgg cgaagctgat ctcgcggctt ccaagcagcg ccgccttcac
     5761 cggaggcatg ccctcctcca catgccggta gatgttctcc aacatgacga tggcgtcgtc
     5821 gaccacaaga ccgatggcca gcaccagcgc gagcagcgtc agcgtgttga tggagaagtc
     5881 cagcgcatac atcagggcga aagcgccgat caacgagacc gggatggtga cgagcgggat
     5941 cagtgtggcg cggaccgagc gcaggaacag aaagatgatg agcacgacga ggaggatcgc
     6001 ctcgccgatg gtgcggtaca cgttcttgat cgactcggcg atgaagatcg atctgtcgtg
     6061 tgcgatgcgc acctccatgc cggcgggcag agattgagtg atgcgcggca gcactgcacg
     6121 cacctcctcc gacacttcca gcgggttggc ggtcgcttgc ttgaccacgc cgagcgcgac
     6181 cgccggcttg ccgttgaagc gcacgatgcg ccgctcgtcg agagccccga gttcggcatg
     6241 gccgacctcg tcgagccgga ccaggtgacc gttggcttcc ttgacgatca gcgcgttgaa
     6301 ttgctccggc gtgcgcaggt cggtctcggc gagcaccgtg aactcgcgct gcatgctctc
     6361 gatgcgcccc gacgggacct cgacattctg gcggcgcaag gcaagctcga tatcctgcgg
     6421 cgagagctga taggcggcga gacgctcggg atcgagccag atgcgcatgg cgtattcccg
     6481 ttcgccaaac aggcggactt cggccacgcc gcttaatgtc tggagctgat ccttcacgta
     6541 gcggtcggcg tagtcggtga tctcgagctc cgagtgccgg tcgctgtaga aggcgagata
     6601 aaggatcggc tgggcgtcgg cctcgacctt ctggatgatc ggctcgtcga tctcgtcggg
     6661 aaggcggccg cgaacgcgcc ctacccggtc gcgcacgtcc gccgcggcat agtcggggtt
     6721 gcgatcgagc ttgaaaatga cggtgatctg gctcgattcc tgccggctga tcgaggtgat
     6781 gaaatcgatg ctctcgatgc ccgccagcga atcttcgagc acctgggtca cctggctctc
     6841 gacgatggcg gcgctcgcgc cgggatagct ggtctggacc gtgaccaccg gcgggtcgat
     6901 tgccgggtac tcgcgcaccg agagccgctg gtagcagacg aggccgagca gcacgagcac
     6961 aaggctcata accgtggcga agaccggccg cttgatcgag agttccggaa gcgtcacggc
     7021 tcacgctccg atggcgtcga cgacggacac aggcgtgccg tcgcggatct tgagctggcc
     7081 ggcggtcacc acctgatcgc cggcggcaag cccttcgacg atctccaccc tgccctcgcg
     7141 ccgcgtgccg gtcacgacct tggtcagctg gaccttgccg tcgacgatct tgaacacgaa
     7201 ctggctgtcg ccttgcggca cgatcgcctg ctcgggcacg ctcaaagcgg cctccttgag
     7261 ctcgaggaga accgtcacgc gcgcgaacag gcccgggcgc agacgcttgt cggcgttcgg
     7321 aacctcggcg cggacaagga gacttcgccc ggagacatcg agctgcggat cgatggcgta
     7381 gaccttgccc tcgaagctat cgtcggggaa agcatcgacc ttgacccgga tggtttgccc
     7441 caccctaatc gccggcaaga atttctcggg caccctgaaa tcggccttta ccggatcgat
     7501 cgcctcgaga ttgaccagcg cctgccccgc ggtgatgtac tcgccgacgc ttacgccgcg
     7561 caggccgacg atgcccgcga acggcgccaa gatatgcgcc ttgtcgagct gaacgcgggc
     7621 gagttccacg gcggcggtgc tgacggcgag attggacgcg gtctcgtcgg ccgactgcgc
     7681 ggtcgcatat ttgctgctga agagctggct tgttcgcttg tgggtctgct cggcgagctt
     7741 aagtttggcc tcggcgtcgg cgagctgggc gcgatagacg gagtcgtcga gcgtgaagag
     7801 cggcgcgccc ttctcgaccg gctgaccttc ctcgaaatgg atctcctcga tgcggccggg
     7861 aatctccgcg ctgatggtga cggattcgtc ggaaagcacc gtacccacgg cggtcacctg
     7921 ctcgctcaac ggcgcggcaa ccactcttgc ggcctcgacc gccacggctt cggtcttgcc
     7981 gtcctgagcc gctgcgccgg ctgcggcaat gccgatagcg gcggccaaga gccagaagcc
     8041 gccccccctc gcaagactgt tccgcctcat gcgatctttc ccgctccgtt gcggcgaaca
     8101 ttttagcagc aatcggggca tcaaggaggt ggggaagccc gccgcagacc ttaaagcttc
     8161 gacatgtctt gccagagcct tggtcggggc ttcctaaaaa gcggaggcgg ccgcc
//
LOCUS       DASUQP010000003         7690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_137601,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000003 DASUQP010000000
VERSION     DASUQP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137601"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1222)
                     /locus_tag="VFY74_00045"
     CDS             complement(266..1222)
                     /locus_tag="VFY74_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX5930135.1"
                     /translation="MAAEPKAPGIKGTPYLREAVEDWLAYLAVERQLAANTAEAYERD
                     ISQFLAFFARERSKLPDMKQLLALSARDVRAFLAARRSEGVGSRSLSRALSALRMFYK
                     FLERRGYGKNDAIRAVSLPKLPHSVPKPLTAPKATALIESADIASPDAPEWVLARDTA
                     VLALLYGSGLRISEALALKRKDAPIKGRDMLRVTGKGGKTRVVPVLPGVREAVERYLA
                     LLPLKPGSEDPLFVGVRGRKLSPRIIQLRIAGARAALGLPETATPHALRHSFATHLLG
                     AGADLRAIQELLGHASLSTTQGYTEVDRAHLLKAYDRAHPRA"
     gene            1319..3745
                     /gene="priA"
                     /locus_tag="VFY74_00050"
     CDS             1319..3745
                     /gene="priA"
                     /locus_tag="VFY74_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5930136.1"
                     /translation="MAKTKTKPKVAPASAPPTVPVLLPLALPAPYDYLLPTGAEAEPG
                     RFVVAPLGTVEYLAAVWRRPADAPAPEIDRKRLREIVEVIEDVPPLPPVSLDFADWVA
                     NYTLSSPGMVLRMMMSAGRAFSPPAPRYGVRLIGPAPERMTPARARVTEVAKNGLIWV
                     KSSLAEAAGVSPGVIDGLVDAGVLTAEPLPDWHALPLDLSRKRATLTAEQNDAARKLL
                     ANTAGGFTVTLLDGVTGSGKTEIYFEAIASALARGRQVLVLMPEIALTGQFIDRCEER
                     FGARPAEWHSGVSAPLRGRIWRAVAENKAKLVVGARSALFLPFPDLGLIVVDEEHDQS
                     YKQEERVTYQARDMAVVRGALGACPVVLSSATPSIESLVNAEQGRYRHIKLAQRFRTG
                     SSEETHPTRLGRDNVRPEPPSPSRGGMKDAAASLTPKPPPGGGELALGLDPRVGAQGA
                     PGGGAEFLTNGGGLPEIAAIDMRRDPPERGRWLSPVMADAISQTLQRGEQALLFLNRR
                     GYAPVTLCRKCGFRFECPNCSAWLVEHRFRRRLECHHCGTFVPIPEACPKCGAEHSLV
                     PCGPGIERIAEEVAERFPDARRVLLSSDLTPNIGDLRETLREIEEREVDVVIGTQLVA
                     KGHHFPGLALVGVVDGDLGLAQADPRAAERTFQLLSQVTGRAGRDAIPGRGLIQTYMP
                     EHPVIQALVSGDRDTFYEREIAARREAGLPPFGRLASLLVSGGAREAVEAYARAVALA
                     APPAAKIQVLGPAEAPLSVIRGRHRYRLLVKAAREANLQAYLRLWLGAVPKSKGDVRL
                     VVDVDPYSFL"
     gene            complement(3759..4520)
                     /locus_tag="VFY74_00055"
     CDS             complement(3759..4520)
                     /locus_tag="VFY74_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HEX5930137.1"
                     /translation="MAKSEIEWTEATWNPVAGCSLVSPGCTNCYAMRMAARLQAMGME
                     KYAGTTRKSGKRHIWTGRVNLDSKSLMAPVTWRKPQRIFVNSMSDLFQEALAEEFVRR
                     VWQVMEDAHWHTFQILTKRPERMLGLLSQPTFRVLPHVWLGTSVENEDHVGRIEFLRR
                     TPAGIRFVSFEPLLAPISNPDLTGIHWAIVGGESGPKARPMEPEWVEDLRDACLRQVV
                     AFFFKQWGGRRKKATGRVLGNRTWDDYPAPAVVAS"
     gene            5702..6262
                     /locus_tag="VFY74_00060"
     CDS             5702..6262
                     /locus_tag="VFY74_00060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX5930138.1"
                     /translation="MAVEEQTVSGVAGRYATALFELALEANAFDQVAADLDRFSAALD
                     ASGHLARLVKSPVFSADEQGRALGAVLDKLEIEGLTRNFLLLVAKNRRLFAAPDMTRA
                     FRALLARHRGETSASVISASKLTEAQTTALKQALKAALGKDVSLEQRVDAGLLGGLVV
                     KVGSRMIDTSLRTRLNSLKVAMKEVG"
     gene            6262..>7690
                     /gene="atpA"
                     /locus_tag="VFY74_00065"
     CDS             6262..>7690
                     /gene="atpA"
                     /locus_tag="VFY74_00065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5930139.1"
                     /translation="MDIRAAEISAILKEQIKNFGQEAEVAEIGQVLSVGDGIARVYGL
                     DKVQAGEMVEFADGTRGMALNLEVDNVGIVIFGDDRHIKEGDTVKRTGAIVEVPVGKG
                     LLGRVVDALGNPIDGKGPIKSDEKRRVDVKAPGIIPRQSVHEPMQTGLKAIDALIPIG
                     RGQRELIIGDRQTGKTAIILDTILNQKSINALGDDKTNLYCIYVAVGQKRSTVAQFVK
                     VLEDNGALPYSIVVAATASDPAPMQFLAPFSGCAMGEFFRDNGMHAVVFYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNKDNGSGSLTALPVIETQANDV
                     SAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSAAQVKAMKQVAGKIKG
                     ELAQYREMAAFAQFGSDLDATTQRLLNRGARLTELLKQPQFSPMTVEEQVVVIYAGVN
                     GYLDPLPVSAVRRFEDELLRYMRAEH"
BASE COUNT         1352 a         2527 c         2398 g         1413 t
ORIGIN      
        1 tcccgctcca atttatgact gactggtcag tactgcaagg tggcctggtg gaattttatg
       61 tgtgtcccgc ctctctatgg ccgggcttgg cccggccatc cacgattacc gcttttccgg
      121 ccaagtcgtg gatgcccggc ctgaagcggg ccatgacgag ctactttgca cggcctcacc
      181 ctgaggaggc cgcggaacgc ggccgtctcc aagggtgcgt gctttcgaga cgggccctcg
      241 gccctcctca gcatgaggga tgctcctaag cgcgggggtg ggcgcggtcg tacgccttga
      301 gcaggtgcgc gcggtcgact tcggtatagc cttgcgtggt cgagaggctg gcgtgcccga
      361 gcagctcctg gatggcgcga agatcggcgc cggcacccaa gagatgcgtg gcgaaggaat
      421 ggcgcagcgc atgcggcgtc gccgtctcgg gcaggccgag cgcggctcgg gcgcctgcga
      481 tcctgagctg gatgatgcgg ggcgaaagtt tcctgccgcg tacgccgacg aacaggggat
      541 cctccgagcc tggcttgagc gggagaagcg caaggtaacg ctcgaccgcc tcccgcacgc
      601 ccggcagcac aggaacgaca cgcgtcttgc cgcccttccc cgtcacccgc agcatgtcgc
      661 ggcctttgat cggcgcatcc ttgcgtttca gcgcgagcgc ctcggagatg cgcaagcccg
      721 agccgtagag cagcgcaagc acggcggtgt cgcgcgcgag cacccattcg ggcgcgtcgg
      781 gcgaggcgat atcggcgctt tcgatcagcg cggttgcttt cggcgcggtg agcggcttcg
      841 gcaccgaatg cggcagcttc ggcaaggata cggcgcggat cgcatcgttc ttgccgtagc
      901 cgcgccgctc gaggaatttg tagaacatgc ggagcgcgct taaagcgcgc gacaggctcc
      961 ggctgccgac accctcgctc cgccgcgccg cgaggaaggc gcgaacgtcg cgcgccgaca
     1021 gcgcgaggag ctgcttcatg tcaggcagtt tgctgcgctc gcgcgcgaag aaggcgagga
     1081 actgcgagat atcgcgctcg tatgcttccg ccgtattcgc cgcgagttgg cgctcgaccg
     1141 cgagataggc gagccagtcc tcgaccgcct cacggagata gggcgtccct ttgatccctg
     1201 gcgccttggg ttctgccgcc aaatcagtga tccccaaatt gaactgtgaa ccctgcccgc
     1261 gcgtgaggct actatgctta gctgagtcgt tagccctccg ttaaatctgc gagcgccctt
     1321 ggccaagacg aagaccaagc ccaaagttgc accggcgagc gcgccgccga cggttcccgt
     1381 acttctgccg ctcgctttgc ctgcgcctta cgactacctg ttgccgacag gcgccgaggc
     1441 cgagcccggc cggttcgtgg tcgctcctct cggcaccgtg gaatatctcg ccgccgtttg
     1501 gcgccgcccg gcggatgcgc ccgcgcccga gatcgaccgc aagaggctgc gcgagatcgt
     1561 cgaagtgatc gaggatgtgc cgccgctccc gccggtctcg ctcgacttcg ccgattgggt
     1621 cgcgaactac acgctctctt ctcccggcat ggtgctgcgc atgatgatga gcgcgggccg
     1681 cgccttctcc ccgcccgcgc cacgctacgg ggtgcggctc attggccctg cgcccgagcg
     1741 catgacccct gccagggcgc gcgtgacgga ggtcgccaag aacgggctca tctgggtcaa
     1801 atcgagcctc gcagaggcgg cgggggtgag tccgggcgtc atcgacggtc tggtcgatgc
     1861 gggcgttctc actgccgagc ctctgcccga ctggcacgcg cttccactcg atctgtcgcg
     1921 caaacgcgcg acgctcacgg cggaacagaa cgatgccgcg cgaaaactcc tcgccaacac
     1981 tgccggcggc ttcaccgtga cgctgctcga cggcgtgacc ggctctggca agaccgaaat
     2041 ttatttcgaa gcgattgctt ccgccttggc gcgcggccgg caggtgctcg tgctgatgcc
     2101 cgagattgct ctcaccggcc agttcatcga ccgttgcgag gagcgcttcg gcgcgcgccc
     2161 ggccgagtgg cattctggcg tgtctgcgcc gttgcgcggg cgtatctggc gcgccgtcgc
     2221 cgagaacaaa gcgaagctcg tggttggcgc ccgctcggcg ctctttcttc cctttcccga
     2281 tctgggcttg atcgtcgtcg atgaggagca cgatcagagc tacaagcagg aggagcgcgt
     2341 cacctatcag gcgcgcgaca tggcggtggt gcgaggggct ctcggcgctt gtcccgtggt
     2401 gctgagctcg gccacgccct cgatcgagag cctggtgaat gccgagcagg gccgctaccg
     2461 ccacatcaag ctcgcccagc gttttagaac gggatcatcc gaagaaactc accccacccg
     2521 gctcggccgg gacaacgtcc ggcccgagcc accctcccct tcaaggggag ggatgaaaga
     2581 tgctgcggct tcgctaacgc caaaacctcc ccctggtggg ggagagcttg ccctcgggct
     2641 tgacccgaga gtcggcgcgc aaggcgcgcc gggtgggggg gcggagtttc tcactaacgg
     2701 cggcgggctg cctgagattg ccgccatcga catgcgaaga gacccgcccg agcgagggcg
     2761 ctggctctct ccggttatgg ctgatgcgat ttcccagacg cttcagcgcg gcgagcaagc
     2821 gctcctcttt ctcaatcgcc gcggctatgc gccggtcacg ctctgccgca aatgcggctt
     2881 caggttcgaa tgcccgaatt gctcggcctg gctcgtcgag cacaggttca ggcgcaggct
     2941 cgaatgccat cattgcggaa cgttcgtgcc gatcccggaa gcatgcccca aatgcggcgc
     3001 cgagcattcg ctcgtgcctt gcgggcccgg catcgagcgc atcgccgagg aggtcgccga
     3061 gcgctttccg gatgcacgcc gcgtgttgct gtcgagcgat ctcaccccga acatcggcga
     3121 tctccgcgag accttgcgcg agatcgagga gcgcgaagtc gatgtcgtca tcggcacgca
     3181 gcttgtcgcc aaaggccatc acttcccggg gcttgcgctc gtcggcgtgg tcgacggcga
     3241 tctcgggctc gcccaggccg acccgcgcgc cgccgagcgc acttttcagc tcctcagcca
     3301 agtgacggga cgcgccggcc gtgacgccat tccgggacgc gggctgatcc agacctacat
     3361 gcccgagcat ccggtgatcc aggcgctcgt ctcgggcgac cgcgacactt tctacgaacg
     3421 cgagatcgcg gcgcggcgcg aagcggggct tccgcccttc ggcaggctcg cctctctgct
     3481 cgtgagcgga ggcgcgcgcg aggcggtaga agcttacgcc cgcgccgtgg cgctcgccgc
     3541 gccgccggcc gcgaagatcc aggtgctggg gcccgccgag gccccgctct cggtgatccg
     3601 cggccgccac cgctacaggc tcctggtcaa ggccgcccgc gaggccaacc tgcaagccta
     3661 tttgcgcttg tggcttggcg ccgtaccgaa gtccaagggt gacgtccggc ttgtcgtcga
     3721 cgtcgatccg tatagttttt tgtaggcacg ctgtgggttc agctagcaac aacagccggc
     3781 gctgggtagt cgtcccaagt gcgatttccg agaacgcgcc cagttgcctt cttccggcgt
     3841 ccaccccatt gcttgaaaaa gaaggctacg acctgccgca agcacgcatc tcgcaggtcc
     3901 tctacccact ccggctccat gggccgggcc tttgggccgg actcccctcc aacaattgcc
     3961 cagtggatgc cggtcaagtc gggattggaa atgggcgcca gcagcggctc gaacgagaca
     4021 aagcgaatgc cggctggggt tcttcgcagg aactctatcc tgccaacgtg gtcctcgttc
     4081 tcgaccgagg ttccgagcca cacgtgtggg agaacgcgga aagttggttg gctgagcaaa
     4141 cccaacatgc gctcgggacg cttggttaga atctggaacg tgtgccaatg cgcatcttcc
     4201 atgacttgcc aaacgcggcg aacaaattct tctgccaagg cctcttggaa gaggtcagac
     4261 atcgaattga cgaaaatacg ttgcggcttt cgccacgtca cgggagccat cagggatttc
     4321 gaatcaagat tgacccgtcc cgtccaaata tgtcgcttgc ctgacttgcg agtcgttccc
     4381 gcatatttct ccatccccat tgcctggagt cgggcggcca tgcgcatcgc gtaacaattg
     4441 gtgcagcccg gcgagacaag cgagcaccca gcgaccggat tccatgtcgc ctcggtccat
     4501 tcgatctccg attttgccac tgagcgcgct ccacaccttt gctaaataag ttgtccctgc
     4561 cctccgggac ttcgaatctt atcccaaaat tggttcgcca attcgtgtcg gccgacaaaa
     4621 atgagccagt aaagtctctg gttctttgtt gttccagata caagctcggc atgcttggcg
     4681 gggggaagtc cgagagccct gttctcagac gcccaatgtg aaaggatccc cgctcggatc
     4741 gcgcgctgtg attggtccag ccgcaccgcg tctctccagc caggcgcgaa acattctagc
     4801 ctgtcatcgc ctgcctggat gtaccgaccg agattccgct gtaggtcttg ggcgctcaga
     4861 tgaatgagca tgtccactcg cttcaatcgg ctaaaggctt tgatgactga aaacgggagc
     4921 gcctgcagat taaatggatc gaggaaaatg aaatgcaagc cggagggatt gagacgcgtg
     4981 acaacatctt ttgcagtttg ttccgctacc cctatgtagg cagtcggcga accacctgct
     5041 cttatcaatc gctgttcggc tgcgcggcac ttgtcggccg aagcgtctgc gatatgcatt
     5101 tgtgagaatg gcgccgccat ctcgggcgca cttgaaagcg agaagcggac tgccgtcgac
     5161 aatctcacca gagtcacgaa ctacggcccg acctctccca cagaacaggt cgacatagct
     5221 tgctccccct ggcccgctaa taaacttggc gcgcactccc cgcgagatgt cgaggtactt
     5281 gcgaagtaga tcgtgcttct gcagggacca tgaccctact tggtcgagcg gtagcccgtc
     5341 ctcgtccagc atccgacgca ccgaaaactt ctacactgca aaattgaatc acaaccgatt
     5401 cacagctgca agggccgcga gcgctcagcc gcccgtagcg ctctcgggtt tgagcctagc
     5461 ggggatcacg ccaagctaat tgcgaaggct aagcaggcct gcaaaacccc tgtctccaga
     5521 ccaatccggg gcatccctcc ggttgcgccc ctctcccccg tatgttagag cctccgcggg
     5581 cttccggggg ctatccaata ggccttcaac ctgttgacgc ggaagcccca atcccagcat
     5641 ttttcggggg tccggggacg gggcggcttt cgatcgagct ctcgttcgga aggaaaaagc
     5701 tttggcggtc gaggaacaga cagtttcggg cgttgccggg cgctatgcga ccgcgctttt
     5761 tgagctcgct ctcgaagcga acgcgttcga ccaagtcgcc gccgaccttg accgcttcag
     5821 cgccgcgctg gatgcctcag gccacctggc gcggctcgtc aagagccccg tctttagcgc
     5881 tgacgagcag ggcagagcgc tcggagcggt cctcgacaag ctcgagatcg aagggctgac
     5941 gcggaatttc cttctcctcg tcgccaagaa ccgccgcctc ttcgccgcgc cggacatgac
     6001 ccgcgccttc cgcgccctgc tcgcgcgcca caggggcgag acgagcgcgt cggtgatctc
     6061 ggcgtcgaag ctcaccgagg cgcaaacgac cgccttgaag caggcgctca aagccgcgct
     6121 cggcaaggac gtgtcgctcg agcagcgcgt cgatgccggc cttctcggcg ggctcgtggt
     6181 caaggtcggc agccgcatga tcgacacttc gctgcgcacg aggctcaatt ctctcaaagt
     6241 tgcgatgaaa gaggttggct aatggatatt cgcgccgcgg agatctccgc gatcctcaaa
     6301 gagcagatca agaatttcgg gcaagaggcg gaagtcgccg agatcggcca ggtgctctcg
     6361 gtcggcgacg gcattgcccg cgtctatggg ctcgacaagg tccaggccgg cgaaatggtc
     6421 gagttcgccg acggcacgcg cggcatggcg ctcaacctcg aagtcgacaa tgtcggcatc
     6481 gtgatcttcg gcgacgaccg ccacatcaag gaaggcgaca ccgtcaagcg caccggcgcc
     6541 attgtcgagg tgccggtcgg caaggggctg ctcggccgcg tggtcgatgc cctcggcaac
     6601 ccgatcgacg gcaagggccc gatcaagtcc gacgagaagc gccgcgtcga cgtgaaggcg
     6661 ccaggcatca ttccccgcca atcggtgcat gaaccgatgc agaccggctt gaaggccatc
     6721 gacgcgctca tccccatcgg ccgcggccag cgcgagctca tcatcggcga ccgccagacc
     6781 ggcaagaccg ccatcatcct cgacaccatc ctcaatcaaa aatcgatcaa cgcgctcggc
     6841 gacgacaaga cgaacctcta ttgcatctat gtcgccgtcg gccagaagcg ttcaaccgtg
     6901 gcgcagttcg tcaaggtgct ggaggataac ggagcgctcc cctattccat cgtcgtcgcc
     6961 gccaccgctt ccgaccccgc gccgatgcag ttcctggctc ccttctctgg ctgcgccatg
     7021 ggcgagttct tccgcgacaa cggcatgcat gccgtcgtgt tctacgacga tttgtcgaag
     7081 caggctgtcg cttatcgcca gatgtcgctc ctgctgcgtc gtccgccggg acgcgaggcc
     7141 tatcccggcg acgtgttcta tctccactcg cggctcttgg agcgggccgc gaagctcaac
     7201 aaggacaacg gctcgggctc gttgaccgcg ctccccgtga tcgagacgca ggccaacgac
     7261 gtgtcggcct atatcccgac caatgtcatc tccatcaccg acggacagat cttcctcgag
     7321 accgatctgt tctatcaggg catccgcccc gcggtgaatg tgggtctgtc ggtgtcgcgc
     7381 gtgggctctg ccgcgcaagt gaaggcgatg aaacaggtgg ccggcaagat caagggcgag
     7441 ctggcgcaat atcgcgagat ggccgccttc gcccagttcg gctccgacct cgacgccacc
     7501 acgcagcgcc tgctcaatcg cggcgcccgc ctgaccgagc ttctcaagca gccgcaattc
     7561 tcgccgatga cggtcgagga gcaggtggtg gtcatctacg ccggcgtgaa cggctatctc
     7621 gatccgctcc ccgtctccgc ggtccggcgt ttcgaggacg agctgttgcg ctacatgcgc
     7681 gcggagcatc
//
LOCUS       DASUQP010000004        22316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1091822, whole genome shotgun sequence.
ACCESSION   DASUQP010000004 DASUQP010000000
VERSION     DASUQP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 22316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22316
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091822"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFY74_00070"
     CDS             <1..813
                     /locus_tag="VFY74_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5930140.1"
                     /translation="AAFMLYVVKDGKTIFADKTVVGTIGYATPVFTAPMTTIVFNPDW
                     NAPETVVKENILPALQRKSYSILKSHKLFVSYNGQPVDATKVDWNRVNGLAYTFSQKA
                     GPHNNLGKVKFLYPNRHTVYMHDTLPVRKKYFKQTMRMIGHECVRMEKPEKFALTLLA
                     EIDGLTEGRIKELWDKGVNSSVALERKFPVHMVYFTAVVDDGGKVETFADVYGLDRKL
                     AVALFGDATGFPQPPPESKKPPPGEYDASNPAARRTAADNDMARAMQGFLGE"
     gene            1021..2235
                     /locus_tag="VFY74_00075"
     CDS             1021..2235
                     /locus_tag="VFY74_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5930141.1"
                     /translation="MIRRFLIAAIIVGLFLGGLGYFNLVFKPKMIGEVMSKMVPPPAT
                     VTTEVAKTESWIERMHAIGTLVAMEGVDVAPQVAGIVTDYFFESGNDVEKGAKLVKLD
                     TSVEEADLADNKATLVQANLDFDRQRNLVDKGAVSQATLDQTVAKRDSALAEVQRVEA
                     IIAQKNITAPFAGRLGLRYVEKGQYVPQGQRLVWLQALDPIWVDFPVPETTIGKFGIG
                     SAVELKADAFPDRLFKGKVEAFDARLSQDSRTLMVRAQVPNPDRTLMPGMFANVAVLA
                     GGEKAFVTVPRTAVTYGLYGDSVWVLKPGAPEPAAAPTPTPTAATDPVASAAAADAAP
                     AGAVPVEPKLTVERRFVRVGPTQGDRVAILEGIKAGDEVVTSGQLKLQPGATVKVDNS
                     GALKPPAELPKQ"
     gene            2239..5514
                     /locus_tag="VFY74_00080"
     CDS             2239..5514
                     /locus_tag="VFY74_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX5930142.1"
                     /translation="MRFTDIFIKRPVLATVVSLLILLIGAQAGFKLPIRQYPELSNTT
                     ITVLTTYPGANADLIQGFITVPIQQAVASAEGIDTLTASSSQNVSTVTLNLKLDADPD
                     RAMTDVLSKIAQVKSILPREANDSVVTKQTGEGYALMYLSFNSKVMTSSQITDYLTRV
                     VQPRLQTINGVANAEILGGQVFAMRIWLDPDRMAALGVTPLDVRAALANNNFTSAPGQ
                     VKGDFVQTNIDAKTALETPEAFGQLVVVGRGDSLVRLGDIAEIELGPENADSSSVFDG
                     LKAVFIGVYATPTANPLTVITDVRKAFPQIQRELPPGLEADIAYDATNFIRASIWEVV
                     KTLGEAALIVIVVIFLFLGNLRSTIIPIVTIPLSLVGVLAMLAALGYSINLMTLLALV
                     LAIGLVVDDAIVVVENIHRHIEQGMKPFDAAILGAHEIFTPIIAMTITLAAVYAPIAF
                     VSGLTGTLFREFALTLAGAVVVSGVIAVTLSPMMCSKLLKAESEHDKQGWLTRFLDRM
                     FEGLKQRYQGRLHRTLNWRPVTMLVLAGIIAATGLMYMTTQRELAPEEDQGILFTIVK
                     TPQYANLDYLEAATDQLYRVFSTVPEKEHVFAINGMGDVHQGFAGILLKPWGERERGH
                     QAILQELQPKIAGVAPAQALTFSPPALPGSVGGPPVQFVITTTRDFRELADVLAEVEK
                     ASRESGMFIFSDSDLRFETPQIEFKIDHDKANRLGITMADIGNSLATLLGGNYVNLFD
                     LYGRSYRVIPQVPREFRLNEEWLTRYQIRTSAGTLVPLSNVASVTKVVQPNALTNFQQ
                     LNSATLSAVPFPGRTVGEAIAFLEKQAENFPEGFTFDFQGESRQFVQEGNTLVYTFVF
                     ALIIIFLVLAAQYESFRDPFIILVALPTSIFGALLPLNAGLGTINIYTQIGLVTLIGL
                     ISKHGILMVDFANKLQEEKGYGRREAIEEAAALRLRPILMTTAATVVAMIPLLIAGGA
                     GAASRFAIGVVIVAGMTIGTMFTLFITPAVYTYLARDHQKARESLAEASAKAEVTVEQ
                     PLEAPGPTTMAATSSHEAQDAALSSEAHAFSSAAAAASSQRQEQRTAKRTGRRRRLPQ
                     AAE"
     gene            5601..7622
                     /locus_tag="VFY74_00085"
     CDS             5601..7622
                     /locus_tag="VFY74_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX5930143.1"
                     /translation="MFSKLLIANRGEIACRIIRTARRLGIKTVAVYSEADRDAIHVDM
                     ADEAVAIGPAQAAQSYLAIDKIVAAAKATGADAIHPGYGFLSENPAFARALDKAGIVF
                     VGPNVKAIEAMGDKIESKKLAAKAKVSTVPGHLGVIEDTKEAVRIAEKIGYPVMIKAS
                     AGGGGKGLRIASSAKEVAEGFASSKSEARSSFGDDRVFIEKYIEEPRHIEIQVLGDKH
                     GNVIHLGERECSIQRRNQKVVEEAPSPFLDAKTREAMGAQAVALAKAVGYDSAGTVEF
                     IVDKNRNFYFLEMNTRLQVEHPVTELVTGLDLVEEMLKVASGEELALKQKDVKLKGSA
                     VEVRIYAEDPERNFLPSTGRLVRYKPPRLGTEDGVTTRLDTGVEEGGEISVYYDPMIA
                     KLITHAPKRLQAIEHMGSALDAFAIDGFRHNIPFLAVLMRNKRWKEGRLSTKFIAEEF
                     PDGFKPPQPKGAVRDTLVAVATVVDHLSNQRRRAITQQMHGELVRFAETRIVELGSER
                     VRVTVAGTLDGPITVTLGGVGTGEKERRIEVASSWWFGAPVWEGTVNGVPAAVQARLI
                     LNGAMLTYQGVEAPARVFTLRESELAGLMPVKERPDTSKFLLCPMPGLMVSVAVTEGQ
                     EVAAGEALCVVEAMKMENVLKAERDSKVAKILAKPGDSLNIDAVILEFA"
     gene            7924..8265
                     /locus_tag="VFY74_00090"
     CDS             7924..8265
                     /locus_tag="VFY74_00090"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5930144.1"
                     /translation="MSKFFERLFGAGMPGKASDRRTVTVKIEGRVQGVFYRAWTEQTA
                     RMLGLDGTVRNMEDGSVEATFSGPAEIVANMLRRCADGPKNARVTKVTVVQEGGSVAP
                     GFRVLGTKWRR"
     gene            complement(8234..8899)
                     /gene="lipB"
                     /locus_tag="VFY74_00095"
     CDS             complement(8234..8899)
                     /gene="lipB"
                     /locus_tag="VFY74_00095"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5930145.1"
                     /translation="MGVTHQAVEWLKSPGLVPYEEAVAAMERRANSIARGDARDCVWL
                     LQHPPIYTVGTSAAPGELLAPERFPVFRSGRGGRFTYHGPGQRVIYVMFDLASRGHDV
                     RAFVRFLEDWLIATLARLGVEGERRSGQIGIFVQGAKIASIGLRVRRWVTLHGASLNV
                     APELEHFSGIVPCGLADTQITSLAALGADADMDMVDDALRAAFDEVSRDQAVSVATSS
                     RAP"
     gene            9077..9162
                     /locus_tag="VFY74_00100"
     tRNA            9077..9162
                     /locus_tag="VFY74_00100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9111..9113,aa:Leu,seq:gag)
     gene            9343..10734
                     /gene="mgtE"
                     /locus_tag="VFY74_00105"
     CDS             9343..10734
                     /gene="mgtE"
                     /locus_tag="VFY74_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5930146.1"
                     /translation="MGQAMQDAAGEVELRDDEGGIRPEFLHAVEAALEAGEAAKLREL
                     TLPLHEADLADLIELLRPEQRTTLISTLGEDFDAAALPELGEAVRDQVLEGMPTEQVA
                     EALQQLDSDEAVYVIEDLDKDEQTDILAKLPHFERLALERSLEYPEDSAGRIMQTDLI
                     AVPPFWSVGQTIDYIGEAEDLPDRFYEVFVVDPAYHLLGSVALNHLLRSKRPNSIDQI
                     TDRDIHPIPVEADQEAVARQFERYNLTSAPVVDGGGRLVGVITADDVVEVVQEEASED
                     ILRMGGVAGESVTDSVWRTMSHRFTWLLANLVTAILASVVIGLFEATIEQMVALAVLM
                     PIVASMGGNAGTQTMTVAVRALATQDLGPANVLRVIWRESAVGLLNGLLFAVIMAAIA
                     FFWFGSDELGLVIGVAMIVNLFAAALAGILIPLGLDALGLDPAIASGVFVTTVTDVVG
                     FFAFLGLAALWLV"
     gene            10749..11759
                     /locus_tag="VFY74_00110"
     CDS             10749..11759
                     /locus_tag="VFY74_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5930147.1"
                     /translation="MFFYLAKVLGFLLQPSTLIALLIGYGSILIWTGWARWGRRFVTI
                     GAILLLLAGLSPLGNALILPLEDRFPRADLHTPPPPAGFIVLGGAEDRLVGDARGAPA
                     LNEAAERMVEAAALSRRYPEARIAFSGGDAGILYRASSEAEGAESLLTTLGIAPDRLI
                     LEAKSRDTYENAALLKEELVRKGELGPGKRWLLITSAYHMPRAMGAFRKAGFEVEAWP
                     VDYRTRGKADLTRPFDKVSEGLRRVDVATREWLGLLAYWLRGRSDALFPAPRPVAVAG
                     CDLALRRPAARQHPHVEPTFDIGGGAELGLVLAGEGRNHFAKHAEADAFRRFVPHHCL
                     LG"
     gene            complement(12090..12371)
                     /locus_tag="VFY74_00115"
                     /pseudo
     CDS             complement(12090..12371)
                     /locus_tag="VFY74_00115"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
     gene            complement(12368..13222)
                     /locus_tag="VFY74_00120"
     CDS             complement(12368..13222)
                     /locus_tag="VFY74_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX5930148.1"
                     /translation="MISEKPPYLGFGLGLRPQHYQEILDGSPAIDWFEIISENYMIEG
                     GQPLYMLDRICERYPTVMHGVSLSIASTAPLDMEYLGALKRLAQRVRPKWISDHLCWT
                     GVHGVNLHDLLPIPYTEEALDHVVARVRQVQDFLGRRLTLENVSSYVTFGQSEMTEWE
                     FVSEVARRADCWLLFDVNNVYVSAFNHGFLTNDFLHGVPRDRVVQFHVAGHSHEESHL
                     IDTHDHPVCDQVWDFYRETVGHFGPVSTMIERDDNIPPLAELVGELDHARSIARAVLG
                     ANRLEAAE"
     gene            complement(13286..13543)
                     /locus_tag="VFY74_00125"
     CDS             complement(13286..13543)
                     /locus_tag="VFY74_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930149.1"
                     /translation="MKLDRKSGAKIAMTAAALLVSGALIGSAQAVELKGRCFGVNGCA
                     GKGACKSAKNDCKGKNACKGEGWIAMLKADCESQGGQFEAS"
     gene            13838..14281
                     /locus_tag="VFY74_00130"
     CDS             13838..14281
                     /locus_tag="VFY74_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEX5930150.1"
                     /translation="MTLHLIKLCVGVDTPRELAEWQADLLKRLAKAGKTPEHCHRTLQ
                     TPKRRREILDGGSLYWVIKGFVLVRQRVIDLRDDMKRDGRSCCGIVLDRELVATRPQP
                     RRAFQGWRYLEAADAPPDAKGFDEEAEAIPRAMREELRELRLIDW"
     gene            complement(14268..15149)
                     /gene="panC"
                     /locus_tag="VFY74_00135"
     CDS             complement(14268..15149)
                     /gene="panC"
                     /locus_tag="VFY74_00135"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5930151.1"
                     /translation="MVAAGASPMSATVVRTVNDLRRGLARPRQARERIALVPTMGALH
                     EGHLSLVALAKSKAERVVASIFVNPLQFGPRDDFHRYPRDEAGDIAKLGEAGADLVFA
                     PDTTEMYPDGFSTSVNVGDLTEDLCGASRPNHFDGVATVVTKLLVQCGPDIAVFGEKD
                     YQQLLVIRRLVRDLNLPVEILGGPIVREKDGLALSSRNAYLSPAEREAAPLLYQTIFN
                     VASDLSKGRGADDAAHAGRFKLEAKGFRVDYVAVRDPETLKPLSGPVKQARVLAAVHL
                     GRTRLIDNVAVPLPTNR"
     gene            15391..16119
                     /locus_tag="VFY74_00140"
     CDS             15391..16119
                     /locus_tag="VFY74_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5930152.1"
                     /translation="MAEPKAPAKPGRSEVAPERSTRGEIDAFLKDVRSLKPMGSGRGR
                     LIFAMDATMSRQPSWDLALELQADMFKTVKAVGGLDVQLIYFRGFNECQASKWVGDPE
                     ALAGLMRKVACAGGFTQIGKVLSHARRESAASKVNALVYVGDCMEEEVDRLANLAGEL
                     GLIGVPVFLFQEGHDPKAERAFREIARLSRGAYCRFDAGSARQLRELLTAVAVYATGG
                     RKALQNFSDETKSNAALRLLEQLG"
     gene            16131..16853
                     /locus_tag="VFY74_00145"
     CDS             16131..16853
                     /locus_tag="VFY74_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5930153.1"
                     /translation="MAFFLVGCALLVLLLLAGRGLAFANTHTLAVAIRKMGGVAALAA
                     AGFLILRGALPLAIPLAVFGFSLLRRSAGFGFGDPFGSARKEPGQKSRIKTDRLEVEL
                     DHDSGRMEGKCLAGRFAGRSLAALSDREAMELLAELHREGAPEAAVMEAYLDWRLPDW
                     REQRSTAGEKSEAGRRRSNGAQRMSAEEAYAVLGIGPKASEEEIRRAHRRLMMKMHPD
                     QGGSNYLAARINEAKEVLLGRL"
     gene            complement(17076..18389)
                     /locus_tag="VFY74_00150"
     CDS             complement(17076..18389)
                     /locus_tag="VFY74_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX5930154.1"
                     /translation="MPVRRGAGLALAFVLVLAGMVASGAEAASRKSRASTPRVAASIV
                     VDMNTGSILHAEAAETPRYPASLTKMMTLYVLFGYLRAGKITPDTELVVTPHAASQAP
                     TKLGLKTGAKIKVSDAAKALVTQSANDAAATIAENLGGSEENFARVMTDTGRRIGMTK
                     TVFRNASGLPNEEQITTARDMAILAAHLIHDYPQYYNVFETKYFTFKGRKYRNHNKLL
                     FGYKGTDGIKTGYTRASGFNLAASVHRGNKHLLAVVLGGRTGSQRDAACRSILDKHFA
                     KASETKPTPEQRVASFVGAPPPSLPAIKKPLLALAAAASAPARLLAPAQGDITEPAPA
                     KRAVPTVSSAAPKTAAKSFRYHGEYHVQVGAYTSQAEAENRLGMVQQRATDLLDGHLP
                     FTASFMKGDEEWYRARFAGFSQDAAKATCKSLKKLSLDCAVMLAE"
     gene            18674..19006
                     /locus_tag="VFY74_00155"
     CDS             18674..19006
                     /locus_tag="VFY74_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5930155.1"
                     /translation="MLKGFEEFQKVGKDGFDAAVRSFGEMNKGFQAIAAEVTDYSKKA
                     FEDSTRAFEQLIGAKTIEQAVEIQSQYAKKAYDSYVAEMSKLGDMYVGLAKDAYKPVE
                     AAFNKKLV"
     gene            19231..19560
                     /gene="clpS"
                     /locus_tag="VFY74_00160"
     CDS             19231..19560
                     /gene="clpS"
                     /locus_tag="VFY74_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX5930156.1"
                     /translation="MARNGDPRRGDDTGTHIITKTRPKTKRPSLYKVLLLNDDYTPMD
                     FVVYVLERFFNKPPEEATRIMLHVHQKGVGICGVYTYEVAETKVAQVTSFSRQHEHPL
                     QCTMEKE"
     gene            19569..22040
                     /gene="clpA"
                     /locus_tag="VFY74_00165"
     CDS             19569..22040
                     /gene="clpA"
                     /locus_tag="VFY74_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX5930157.1"
                     /translation="MASFSTSLEQALRRALQYANERSHEYATLEHLLLSLLDDRDAAA
                     VMRACDVDLDALRDKLTGYVDNELSAMVLDSRGDAQPTAAFQRVIHRAVAHVQSSGRE
                     EVTGANVLVAIFAERESHAAFFLQEQHMTRFDAINYISHGIAKRAGMSETRTVRGVDE
                     DAATVEAGDEKKRGADALEVYCINLNQKARAGRIDPLIGRGPEIQRTIQVLCRRQKNN
                     PLFVGDPGVGKTAIAEGLARRIVMGDVPEVLSDSTIFQLDMGALLAGTRYRGDFEERL
                     KAVMREIEAYPGAIMFIDEIHTVIGAGATSGGAMDASNLLKPALQTGTLRCIGSTTYK
                     EYRQHFEKDRALVRRFQKIDVNEPSVPDAIEILKGLKPYFEDFHRVRYTSQAIKSAVE
                     LSAKYIHDRKLPDKAIDVIDETGASQMLVPERQRRKTIGVKEVESTIATMARIPPKTV
                     SKSDAEVLRAIDQDLKRVVFGQDQAIEALAAAIKLSRAGLREPGKPIGCYMFSGPTGV
                     GKTEVARQLALLLGVELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDGVDQHP
                     HCVLLLDEIEKAHPDLFNILLQVMDHGKLTDHNGKRVDFQNVILIMTTNAGASDLAKA
                     AIGFNRTKREGDDEEAINRMFSPEFRNRLDAVIAFASLTPEVISKVVEKFVFQLEAQL
                     GDRNITIELTPEANKWLAERGYDDKFGARPLARVIQEQIKKPLAEEILFGALKDGGIV
                     RVLVEEKGPEKALGFEYLKPDTPVRPRDPEENEDEGPTDGEPKGPPKSGAPKPRAKRG
                     ARKPKGGRDRSVGASLVPKVPLGSK"
BASE COUNT         4009 a         7158 c         7240 g         3909 t
ORIGIN      
        1 gccgcgttca tgctctacgt ggtcaaggac ggcaagacga tcttcgccga caagacggtg
       61 gtcggcacca tcggctacgc cacgccggtc ttcaccgcgc cgatgacgac aatcgtattc
      121 aatcctgatt ggaacgcgcc cgagacggtg gtgaaggaga acatcctgcc ggctttgcaa
      181 cgcaagagct actcgatcct gaaatcccac aagctcttcg tgagctacaa cggtcagccg
      241 gtggacgcga ccaaggtcga ctggaaccgc gtgaacggtc tcgcctacac cttctcgcag
      301 aaggccgggc cgcataacaa tctcggcaag gtgaagttcc tctaccccaa ccgccacacc
      361 gtttatatgc acgacacgct ccccgtgcgg aagaaatact tcaagcaaac gatgcgcatg
      421 atcgggcacg aatgcgtgcg catggagaag cccgaaaaat tcgccttgac ccttcttgcc
      481 gagatcgacg gcttgacgga aggccgcatc aaggagctgt gggacaaggg cgtcaacagc
      541 tcggtcgcgc tcgagcggaa gttcccggtg cacatggtct acttcacggc ggtggttgat
      601 gacggcggca aggtcgagac gttcgccgac gtctacgggc tcgaccggaa gctcgccgtc
      661 gccctgttcg gcgatgcgac cggatttccc cagccgccgc ccgagagcaa gaagccgccg
      721 cccggagagt acgacgcctc gaaccccgcg gcgcggcgca cagcggccga caacgacatg
      781 gcgcgggcca tgcagggctt cctcggcgaa tagcgtaagg ctttcgccaa aaggcccaaa
      841 ttcagccgcg aatcctcgcc attgacaatc gcttgaccct ggcggcccat tcggtttgca
      901 aacggtgaaa gggccgctta cgtattggca acgtgccccg ggtaggggcg caatttggcc
      961 ttctccggcg gctagtttag agcgctcgcc ctggggcacg aggccgcaag caatagcacc
     1021 atgatcaggc gctttctcat cgccgccatt atcgtcggcc tgttcctcgg cggacttggc
     1081 tacttcaatc tcgtcttcaa gccgaagatg atcggcgagg ttatgtcgaa gatggtgccg
     1141 ccgccggcga cggtgaccac ggaggttgcc aagaccgaga gctggatcga gcggatgcac
     1201 gccatcggca cgcttgtcgc catggaaggc gtggatgtcg cgcctcaggt ggccggcatc
     1261 gtgaccgact atttcttcga aagcggcaac gatgtcgaga agggcgcgaa gctggtgaag
     1321 ctcgatacct cggtggaaga ggcggatctc gccgataaca aggcaacact cgttcaagcc
     1381 aatctcgatt tcgatcgtca aaggaacctc gtggacaagg gcgccgtctc gcaggccacc
     1441 ctcgatcaga ccgtcgccaa gcgcgattcg gccttggcgg aggtgcagcg cgtcgaggcc
     1501 attatcgcgc agaagaacat cacggcgccc ttcgccggac ggctcggcct gcggtatgtg
     1561 gagaagggac aatacgttcc gcaaggacag cgcctggttt ggctgcaagc gctcgacccg
     1621 atctgggtcg actttcccgt acccgagaca acgatcggca agttcggcat cggaagcgca
     1681 gtcgagctca aggccgacgc tttcccggat cgtctgttca agggcaaggt ggaggccttc
     1741 gatgcgcgcc tcagccaaga tagccggacc ttgatggttc gggcgcaagt ccccaatccc
     1801 gatcgcacgc tcatgcccgg catgttcgcc aatgtcgccg tgctcgcggg gggagagaag
     1861 gcgttcgtca ccgtgccgcg cacggcggtg acctacggtc tttatggcga tagcgtctgg
     1921 gtgctgaagc cgggcgcgcc ggagcctgcc gcggcgccga cgccgacgcc gaccgcagcg
     1981 accgacccgg tcgcctccgc agcggccgcc gatgctgccc cggcaggcgc cgttccggtc
     2041 gagcccaagc tcacggtgga gcgccgcttc gtgcgcgtcg ggccgacgca aggcgaccgg
     2101 gttgccattc tcgagggaat caaggccggc gatgaggtgg tcaccagcgg ccagctcaag
     2161 ctgcagccgg gcgccaccgt caaagtcgac aattcgggcg cgttgaagcc gccggcggag
     2221 ctgcccaagc aataatccat gcgcttcacc gacatcttca tcaagcggcc tgtgctcgcc
     2281 acggtcgtca gtctgctgat cctcctcatc ggggcgcagg ccggcttcaa gctgccgatc
     2341 cgtcagtatc cggaactgtc caacaccacg atcacggtcc tcacgaccta tccgggcgcc
     2401 aatgccgatc tgatccaggg cttcatcacc gtgccgatcc agcaggcggt ggcgagcgcc
     2461 gaaggcatcg atacgctgac cgcgtcatcg tcacagaatg tgagcacggt cacgctcaac
     2521 ctgaagctcg atgccgatcc cgaccgggcc atgaccgacg ttttgtcgaa gatcgcccag
     2581 gtgaagagca tcctgccgcg cgaggcgaac gattccgtgg tcaccaagca gaccggagag
     2641 ggctacgcgt tgatgtacct ctccttcaat tccaaggtga tgacgagctc gcagatcacc
     2701 gactacctta cccgcgtcgt gcagccgcgg ctccagacca tcaatggcgt agccaatgcc
     2761 gagattctcg gcgggcaggt cttcgccatg cgcatctggc tcgaccccga ccgcatggcc
     2821 gcgctcgggg tgacgccgct cgatgtgcgg gcggcccttg ccaacaacaa cttcacctcg
     2881 gccccgggtc aggtgaaggg cgacttcgtg cagaccaata tcgacgccaa gaccgcgctc
     2941 gagacgcccg aggccttcgg tcagctcgtg gtggtcgggc gcggcgattc cctggtcagg
     3001 ctcggcgaca tcgccgagat cgagctcggg ccggagaacg ccgattcttc ctcggtcttc
     3061 gatgggctga aggcggtttt catcggcgtt tacgccacgc cgaccgcgaa cccgctgacg
     3121 gtcatcaccg acgtccgcaa ggcttttccg cagattcaac gcgagctgcc ccccgggctc
     3181 gaagcggaca tcgcctatga cgccaccaac ttcatccgcg cctcgatctg ggaggtggtg
     3241 aagaccttgg gcgaggcggc cctcatcgtc atcgtcgtca tcttcctgtt cctcggcaat
     3301 ctcaggtcga cgatcattcc gatcgtgacg atcccgctgt cgctggtcgg cgtgctcgcg
     3361 atgctcgcgg cgctcgggta ctcgatcaat ctgatgacgc tgctcgcgct cgtgctcgcc
     3421 atcggattgg tggtggacga cgccattgtc gtggtcgaga acatccaccg tcatatcgag
     3481 caggggatga agccgttcga cgcggccatt ctcggcgcgc acgagatttt cacccccatc
     3541 atcgccatga ccatcacgct cgcggcggtc tacgcgccga tcgcgttcgt gtcggggctc
     3601 accggcacgc tgttccgcga gttcgcgctc actctcgccg gcgcggtggt ggtgtccggc
     3661 gtcatcgccg tgacgctctc gccgatgatg tgctcgaagc tgctaaaggc ggagagcgag
     3721 cacgacaagc agggctggct cacgcggttt ctcgaccgga tgttcgaggg cctgaagcag
     3781 cgctaccagg ggcgccttca tcgcacgctg aattggcgcc ccgtgaccat gctcgtcctc
     3841 gccgggatta ttgccgcgac cggcctcatg tacatgacca cgcagcgtga gcttgcgccc
     3901 gaggaagacc agggcatcct gttcaccatc gtcaagacgc cgcaatacgc caatctcgat
     3961 tatctcgaag cggcgaccga ccagctctat cgcgtcttca gtaccgtgcc ggagaaggaa
     4021 cacgtgttcg ccatcaacgg tatgggtgac gtgcatcaag ggttcgcggg catcctgctc
     4081 aagccctggg gcgagcgaga gcgcggtcac caagccatcc tccaggaact gcagccgaag
     4141 atcgcggggg ttgcgcccgc gcaggcgctg acgttctcgc cgccggcctt gccgggctcg
     4201 gtgggcggac cgccggtcca attcgtcatc accacaacgc gcgacttccg cgagcttgcc
     4261 gatgttcttg ccgaagtcga gaaggcgtcg cgcgaaagcg gcatgttcat cttttcggac
     4321 tccgatctcc gtttcgagac gccgcagatc gagttcaaga tcgatcacga caaggcgaac
     4381 cggctcggca tcaccatggc cgatatcggc aattcgctcg caaccctgct cggcggcaac
     4441 tacgtcaacc tgtttgatct ttacgggcgc agctatcggg tcatcccgca ggtgccgcgc
     4501 gagttcagac tgaacgagga atggctgacc cgctatcaga tccgaacgag cgcgggcact
     4561 ctggtgccgc tgtccaacgt ggcgtcggtc accaaagtgg tgcagccgaa cgcgctgacc
     4621 aacttccagc agctcaactc cgcgacgctc tcagccgtgc cgttccccgg gcgaaccgtc
     4681 ggcgaggcga tcgcctttct cgagaagcaa gccgagaatt tcccggaagg cttcaccttc
     4741 gacttccagg gcgagagccg gcagtttgtg caggagggaa acactctcgt ctatacattc
     4801 gtgtttgcgt tgatcattat cttcctggtg ctggcagcgc aatatgagag cttccgcgac
     4861 cctttcatca tcctggtcgc gctgccgacc tcgatcttcg gggcgctcct cccgctcaat
     4921 gccgggctcg gcaccatcaa catctacacg cagatcgggc tcgtgacgtt gattgggctg
     4981 atctcgaagc acggcatcct gatggtggac ttcgccaaca agctccagga agaaaagggc
     5041 tacggccggc gcgaggcgat cgaggaggcc gcggccttgc ggctgcggcc gatcctgatg
     5101 acgacggcgg caaccgtggt cgccatgatc ccgcttctga tcgccggcgg tgcgggcgcg
     5161 gccagccgct tcgccatcgg cgtggtgatc gtcgcgggca tgaccatcgg cacgatgttt
     5221 accctgttca tcacgcccgc ggtctatacc tatcttgcca gagaccacca gaaggcccgg
     5281 gagtcgcttg ccgaagcgtc cgcgaaggcc gaagtgacgg ttgagcagcc ccttgaggcg
     5341 cccggtccca caacgatggc ggcaacgtct tcgcacgaag cgcaggacgc cgctctgtcc
     5401 tcagaagccc atgccttcag ctcggcggcc gccgcagcat cgagccaacg ccaagagcag
     5461 cgtacggcca agcgtacggg acggcggcgg cgcttgcctc aagccgctga ataggcttcc
     5521 ccccagcacc cgccatctag tttactcggt tccggtgaac ctctagaaat cgccctagca
     5581 gcagggggcc cgggacccgc atgttctcca agcttctgat cgccaatcgg ggcgaaatcg
     5641 cctgccgtat catcaggacg gcgcgccggc tcggcatcaa gaccgtcgcc gtctattccg
     5701 aggccgaccg cgacgcgatc cacgtcgata tggccgacga ggcggtggca atcggccccg
     5761 cccaggccgc gcagtcctat ctcgcgatcg acaagatcgt ggccgccgcc aaggcgacgg
     5821 gggcagacgc catccatccg ggctatgggt ttctctccga gaaccccgca ttcgcgcgcg
     5881 cgctcgacaa ggccggcatc gtgttcgtcg gaccgaacgt caaggccatc gaggcgatgg
     5941 gcgacaagat cgagtcgaag aagctcgccg ccaaggccaa ggtctcgacc gtgccgggcc
     6001 atctcggggt gatcgaggac accaaggagg cggtcaggat tgccgagaag atcggctatc
     6061 cggtcatgat caaggcctct gccggcggcg gcggcaaggg cctgcgcatc gcctctagcg
     6121 ccaaagaggt cgccgaaggc ttcgcgtctt cgaagagcga ggccagatcg agcttcggcg
     6181 acgatcgcgt gttcatcgag aaatatatcg aggagccgcg gcacatcgag atccaagtgc
     6241 tcggcgacaa gcatggcaat gtcatccatc tcggcgagcg cgaatgctcg atccagcggc
     6301 ggaaccagaa ggtggtcgag gaggcgccga gcccgttcct cgatgccaag acgcgcgagg
     6361 cgatgggggc gcaagccgtc gcgctcgcca aggccgtcgg ctacgattcg gccggcaccg
     6421 tcgagttcat cgtcgacaag aaccgcaact tctacttcct cgagatgaac acgcggctcc
     6481 aagtcgagca ccccgttacc gaactcgtca ccgggctcga tctcgtcgag gagatgctga
     6541 aagtcgcctc aggcgaggag ctcgcgctca agcagaagga cgtgaagctc aagggctcgg
     6601 ccgtcgaggt gcgcatctac gccgaggatc cggagcggaa cttcctgccg tcgacggggc
     6661 ggctcgtgcg ctacaagccg ccaaggctcg gaaccgagga cggcgtcacc acgcggctcg
     6721 atacgggcgt ggaggagggg ggcgaaatct cggtctatta cgatccgatg atcgccaagc
     6781 tcatcaccca tgcgccgaag cggctgcaag cgatcgagca tatgggaagc gcgctcgatg
     6841 cgttcgccat cgacggattt cgccacaata tcccgttcct cgccgtgttg atgcggaaca
     6901 agcgttggaa ggaaggccgc ctttccacca agttcatcgc cgaggagttt ccggacgggt
     6961 tcaagccgcc gcagcctaaa ggcgccgttc gcgacacgct ggtcgcggtc gcgaccgtcg
     7021 tcgatcattt gagcaatcag cggcgccgcg ccatcacgca gcagatgcac ggcgagctcg
     7081 tgcgcttcgc cgagacgcgc attgtcgaac tcggctcgga gcgggtgcgc gtcaccgtcg
     7141 ccggcaccct tgacggtccc atcaccgtga cgcttggcgg cgtggggacg ggcgagaagg
     7201 aacggcgcat cgaggtcgca tcgtcctggt ggttcggtgc ccccgtatgg gaggggacgg
     7261 tcaacggcgt gcccgccgcc gtgcaggcgc gcctgatcct gaacggcgcg atgcttacct
     7321 atcagggcgt cgaagcgccg gcgcgcgtct tcaccttgcg cgagagcgag ctcgcgggcc
     7381 tgatgccggt caaggagcgg cccgatacgt cgaagttcct gctctgcccg atgccggggc
     7441 tcatggtttc ggttgccgtg actgaaggcc aggaggtcgc ggcgggggag gcgctctgcg
     7501 tggtcgaggc gatgaagatg gagaacgtgc tgaaggccga gcgcgactcc aaggtggcca
     7561 agatcctggc aaagccaggc gacagcctca atatcgatgc cgtgatcctc gaattcgcct
     7621 aaaggctcgt cacctcccga aacctcttcg tcggctctcc cccctgcctt cgccgaagcg
     7681 aagccgctcg gcttcgcgca ggcaggttgg ggagagcacg cgcgccgttc ggcgcgcggt
     7741 gaggggagcc gcgacgcgct ggccaagaat cgccctcacc cttcccgcca tgcgacttgc
     7801 gcagagctgc gctcgcgcaa gatcgcgggc ggcaagccct ctccctcagg ggagaggggg
     7861 cactttcgcc attgccgcgc ttgaccagac agggaaatag tctcaagccg agcgtcgagg
     7921 gtcatgagca agttcttcga acgtctcttc ggggcgggca tgcccgggaa ggcatctgac
     7981 aggcggactg tgaccgtcaa gatcgagggc cgcgtgcagg gcgtcttcta ccgcgcctgg
     8041 accgagcaga cggcgcgcat gctcggcctt gacggcacgg tgcgcaatat ggaggacggg
     8101 agcgtcgagg ccacgttttc aggtccggcc gaaattgtcg ccaacatgct gcggcgctgc
     8161 gccgacgggc ccaagaatgc gcgggtcacc aaggtgacgg tggtgcaaga aggcggttcg
     8221 gtcgcgccgg gcttcagggt gctcgggacg aagtggcgac gctgactgcc tgatcgcgcg
     8281 acacctcatc gaaggcggcg cggagcgcgt catcgaccat gtccatgtct gcgtcggcgc
     8341 cgagtgcggc gaggctcgtg atttgcgtat cggcaagccc gcacggaacg atgcccgaga
     8401 aatgctcgag ctccggcgcg acgttgaggc tcgcgccgtg cagcgtcacc cagcggcgca
     8461 cgcggaggcc aatgctcgcg atcttcgccc cttgcacgaa aatgccgatc tgaccggacc
     8521 gccgctcgcc ctcgacgccg agccgcgcca gtgtcgcaat cagccagtct tcgagaaagc
     8581 ggacgaaggc cctgacatcg tgtccgcgcg acgcgaggtc gaacattaca tagatcacgc
     8641 gctggccggg accgtgatag gtgaagcgcc cgccgcgccc gcttctgaac acggggaacc
     8701 tctccggcgc gagaagctcg cccggcgcgg cactggtgcc gacggtatag attggcggat
     8761 gctgcaagag ccacacgcag tcccgcgcgt cgccgcgggc gatggaatta gcgcgccgtt
     8821 ccatggcggc gacagcctcc tcatagggca cgaggcccgg gctcttcagc cattcaaccg
     8881 cttggtgcgt aacgcccaag agcacttcgc gtctcgccga aagcactgcc ataagccggg
     8941 ttgtgaaggc caaaacccaa gctggcaagc ctctccttgc accccctgag caggtttgct
     9001 aaggcacctc ggccttgctt gaaagcgggg tcagactaag ccatatgccg gtagcgcggg
     9061 tgctccggaa ctccttgcgg tcgtggcgga attggtagac gcgcagcgtt gaggtcgctg
     9121 tgggctatgc ccgtggaggt tcgattcctc tcgaccgcac cactcccctt ccggggacca
     9181 tgagcggcgc ttttttggct ggaaattccc tcctaacaag gctctagtgg ctgccgagcc
     9241 gcgcctgtca tcgcaggccc ggaagcggga attcgtccct ttcagcgccg ttcccgcttc
     9301 cgcggacccc gcggccttcc cgcttgacgc ttaaccccgg tgatgggaca ggccatgcag
     9361 gatgccgcgg gcgaggtcga actgcgcgac gatgagggcg gaatccgccc cgagttcctg
     9421 cacgccgtcg aagcggcgct cgaggccggc gaggcggcga aactgcgcga gctcaccctt
     9481 ccgctgcacg aggccgacct cgccgatctc atcgagctgc tccgtcccga gcagcgcacg
     9541 acactcatct cgacgctcgg cgaggatttc gacgcggcgg cgctgcccga acttggcgag
     9601 gccgtgcgcg atcaggtgct cgagggtatg cccaccgagc aggtggccga ggcgctgcag
     9661 cagctcgatt ccgacgaggc cgtgtacgtc atcgaggatc tcgacaagga cgaacagacc
     9721 gacatcctcg ccaagctccc gcatttcgag cgcctcgcgc tcgagcgcag cctcgaatac
     9781 ccggaggatt cggccggccg catcatgcag accgacctca tcgccgtgcc gcccttctgg
     9841 tccgtggggc agacgatcga ctacattggc gaggctgaag atctgccgga ccggttctat
     9901 gaggtcttcg tcgtcgatcc cgcctatcat ctgctcggct cggtcgcgct caatcatctg
     9961 ctccggtcga agcgtccgaa ctcgatcgac cagatcaccg atcgcgacat ccatccgatc
    10021 ccggtcgagg ccgatcagga ggcggtggca cgccagttcg agcgctataa cctcacctca
    10081 gcgcccgtgg ttgacggcgg cggccggctt gtcggcgtga ttaccgccga cgacgtggtc
    10141 gaggtcgtgc aggaagaggc gtcggaagac atcttgcgca tgggcggcgt ggccggagag
    10201 tcggtcaccg actcggtctg gcgcaccatg agccaccgct ttacctggct gctcgccaat
    10261 ctcgtcaccg ccattctcgc ctccgtcgtg atcggcctgt tcgaggcgac gatcgagcag
    10321 atggtggcgc tcgccgtgct gatgccgatc gtggcctcga tgggaggcaa tgccggcacc
    10381 cagaccatga cggtcgcggt gcgggcgctt gccacgcagg acctcggtcc cgccaacgtg
    10441 ctcagggtca tctggcgcga atccgccgtc ggcctgctca acggattgct cttcgccgtc
    10501 atcatggccg cgatcgcgtt tttctggttc ggcagcgacg agcttggcct cgtgatcggg
    10561 gttgccatga tcgtgaacct gttcgccgcg gcgctcgccg gcatcctcat cccgctaggt
    10621 ctcgacgcgc tcggtctcga tcccgccatc gcttccggcg tattcgtcac cacggtgacg
    10681 gacgtggtcg gcttcttcgc cttcctcggg cttgccgctt tgtggctggt gtaggggcgg
    10741 cgcctcgcat gttcttctat ctcgccaagg tgcttgggtt cctgctccag ccctccacgc
    10801 tgatagcgct tctgatcggc tatggttcga tcctgatctg gacaggatgg gctaggtggg
    10861 gccggcgctt cgtcacgatc ggggccattc tgctgctgct cgccggtcta tcgccgctcg
    10921 gcaatgcgct gatcctcccg ctcgaggacc ggtttccgcg cgccgatctc catacgccgc
    10981 cgccgcccgc gggcttcatc gtgctcggcg gcgccgagga caggctcgtg ggggatgcgc
    11041 gcggcgcgcc cgcgctgaac gaggccgccg agcggatggt cgaggccgcg gccctgtcac
    11101 gccgctaccc ggaggccagg atcgccttct cgggcggcga tgccggcatt ctttatcggg
    11161 ccagcagcga ggccgagggc gccgagtctc tgctgaccac gctcggcatt gcgccagatc
    11221 ggctcatcct cgaggccaag tcgcgcgaca cttacgaaaa cgcggctctt ctcaaggagg
    11281 agcttgtccg caaaggcgag cttggcccgg gcaagcgctg gctcctcatc acctccgcct
    11341 atcacatgcc gcgggcgatg ggcgctttcc gcaaggcggg cttcgaggtc gaggcctggc
    11401 cggtcgatta tcgcacgcgc gggaaggccg acttgacgcg gcccttcgac aaggtctccg
    11461 agggcctgcg ccgggtcgat gtcgccaccc gcgaatggct tgggcttctc gcctattggc
    11521 tcaggggacg gagcgacgcg ctgtttccag cgccccggcc ggtcgccgtc gcgggctgcg
    11581 acctggcgct acgccgtccg gcagcccgcc agcatcccca tgtcgaacca acctttgaca
    11641 taggaggcgg cgcggagctc ggcctcgtcc tcgccggcga aggtcgcaac catttcgcaa
    11701 agcacgccga agcggacgcc tttcgccgct tcgtcccaca tcattgcctc ctcggctgaa
    11761 agcggtcgga agcgcgacat gaagtcctgg cgccagacca gggtcgcctg cggcgcgggc
    11821 agatgccgag ctttgggcgg ggcggcctcg cccttcagcg cggaccagat atcggcggcg
    11881 ttggttgcga acgtgagccg gatcgcggtc ggatgcggca cgaagacgag gttgggccaa
    11941 gcctcgggtg cgagcgcggc aagcgcctcg atgcgaagcg ggtcggcatc gggaccgtcg
    12001 aaggcatcgg tcagcgcctt ctccagttcg gcgatctcgg cgacctcgcg gtggttggcg
    12061 taggtctcgc cgtttctcac gaaggcggga aggtggcgcc cgaaccaacg cgcgctcctc
    12121 tggtcggagg gattggcggc aatgtaggcc ttcaccagct tggcgaaggc ccgatcgccg
    12181 agataggcgt gcagctgctc gtagtcctcg ccgaggatat ccgcgaggcg cgccacatag
    12241 gcattgcgat agacgccgaa cagcgctttt cgccggtcct tggcgctgtc cttgacctcg
    12301 gtaaggatag cgtcgtcgcc ggcaaggatg ccgcgctgga agctctcttg gatctcgcga
    12361 aggctcttca ttccgcggct tcgagccgat ttgcgccgag caccgcgcgg gcgatcgagc
    12421 gcgcatggtc gagctcgccg acaagctcgg cgagcggcgg gatgttgtcg tcgcgctcga
    12481 tcatggtcga caccgggccg aaatggccga ccgtctcacg gtagaaatcc cacacctggt
    12541 cgcacaccgg atggtcgtgc gtgtcgatga ggtggctctc ctcgtggcta tgaccggcga
    12601 catggaactg gaccacgcgg tcgcgcggca cgccgtgcag gaagtcgttc gtcaagaatc
    12661 cgtggttgaa ggcgctcaca tagacgttgt tgacgtcgaa caggagccag caatcggcgc
    12721 gcctcgcgac ctcgctcacg aactcccact cggtcatctc ggattggccg aaggtgacgt
    12781 agcttgacac gttctcgagc gtcagccggc gtccgagaaa atcctgaacc tggcggacgc
    12841 gcgcgacgac gtgatccagc gcttcctcgg tgtaagggat gggcaagaga tcgtgcaggt
    12901 ttaccccatg cacgccggtc cagcagagat gatcggagat ccatttcggc ctgacgcgtt
    12961 gggcgagccg cttgagagcg ccgagatact ccatatcgag cggcgcggtc gaggcaatcg
    13021 agagcgaaac gccgtgcatc actgtcggat agcgctcgca aatgcggtca agcatgtaga
    13081 gcggctggcc gccctcgatc atgtaattct cggagatgat ctcgaaccaa tcgatggcgg
    13141 gggagccgtc gaggatctcc tgataatgct gcggcctgag ccccaagccg aagccgagat
    13201 agggaggctt ctcgctgatc atgccccaag ttcccgcagt ttgggaggcc ggccgcgaat
    13261 gaagcgatcg gcctgcgcca aaatgttagc tcgcttcgaa ctggccaccc tggctctcgc
    13321 aatccgcttt tagcatcgcg atccagcctt cccccttgca ggcattcttg cccttgcagt
    13381 cgttcttcgc gctcttgcag gcgcccttgc cggcgcagcc gttgacgccg aagcagcgcc
    13441 ccttcaactc cacggcttga gcgctgccga tcaacgcccc ggaaacgagc agagccgccg
    13501 cggtcatggc aatcttcgcg ccggattttc gatcgagctt catgttccgc ctcctgtgca
    13561 tgaggtctaa gcagtcgtgg cggcgagccg ccaagcgagc tatagccttg tttgatcggg
    13621 ggccctgcgt cactcctgtt caaagctgtg tgaccctcgg ccttaaccgt gccagctgtg
    13681 gtaaccgttc gtgatcggag cgttgccagg aatgatagcg agtatcgacc aaggccctca
    13741 aggctattcg ttcgttgaat ccgcggggtt ttggcaaagc aacatttgcc gcgggacgac
    13801 ccacgagcat cccggcggtc atgacgcatg agggctcatg acgctgcatc tgatcaagct
    13861 ttgtgtcggc gtcgatacgc cgagggagct tgccgagtgg caggcggacc tgctcaagcg
    13921 cctcgcgaag gcgggaaaga cgccggagca ttgccatcgc actctccaga ctccgaaacg
    13981 gcgccgtgaa atcctcgacg ggggctcgct ctattgggtg atcaagggct tcgtgctggt
    14041 gcggcagcgc gtgatcgatc tccgcgacga tatgaagaga gacggccgga gctgttgcgg
    14101 catcgtgctc gaccgagaac tggtggccac ccggccgcaa ccgcgccgcg ctttccaggg
    14161 ctggcgctat ctcgaagcgg ctgacgcgcc gccggacgcc aaaggtttcg acgaagaggc
    14221 cgaggcgatt ccgcgggcga tgcgcgaaga attgcgcgag cttcgcctca tcgattggta
    14281 ggcaggggca cggcgacgtt gtcgatgagc cgcgtccgcc cgagatgcac cgccgccagc
    14341 acgcgcgctt gcttcaccgg gcccgacagc ggcttcaacg tctcggggtc gcgcacggcg
    14401 acatagtcga cgcggaaacc ctttgcctcg agcttgaagc gaccggcgtg cgcggcgtca
    14461 tcggcgccgc ggcccttgct caggtctgag gccacgttga agatggtttg atagagcagg
    14521 ggagcggcct cgcgctccgc cggcgacaga taggcgttcc gcgaggacag cgcgagaccg
    14581 tccttctcac gcacgatcgg accgccaagg atctcgacag ggagattgag gtcgcgcacg
    14641 aggcgcctga tcacgagcag ctgctgatag tccttctcgc cgaagacggc gatgtcgggg
    14701 ccgcattgca ccaggagctt ggtcacgacg gtagcgacgc cgtcgaaatg gttgggccgg
    14761 gaggccccgc atagatcctc ggtcaggtct ccgacattca cgctggtgct gaagccgtcg
    14821 ggatacatct cggtggtgtc gggggcgaag acgaggtcgg caccggcttc gccgagcttg
    14881 gcgatatcgc cggcttcgtc gcgggggtag cggtggaaat cgtcgcgcgg accgaactgg
    14941 agcggattga cgaagatcga ggcgacgacg cgctcggcct tgctcttggc gagcgcaacc
    15001 agcgatagat gaccttcgtg cagtgcgccc atggtgggca cgagcgcgat cctttcgcgt
    15061 gcttgccgcg gacgggcaag gccacgcctc aggtcgttta ctgtacgaac gacggtggcg
    15121 ctcatagggc tggctccggc ggcgaccata aaggggcttg ccctcacgtc aactgccggg
    15181 cccttctgcc ggcgccctag cttcatacct ccccacgagg ggaggttgaa aggacgcatt
    15241 accaaagtca gactcgggtc tccagttgca aggggaggga tggattctca gcaaggctgg
    15301 gagaggagaa agtgcgatcc ggcattgttc gcaaccggac gagggtccat ctatactccg
    15361 aactgtcgtc tcttggagtt acgacgagaa atggccgagc caaaagcccc cgcgaagccc
    15421 ggtaggagcg aggtcgcgcc ggagcgctcg acgaggggcg agatcgatgc cttcctcaag
    15481 gacgtccgca gcctgaagcc gatgggctcg ggccgcggcc ggctgatctt cgccatggac
    15541 gcgaccatga gccgccagcc gtcctgggac ctcgcgctcg aactccaggc cgacatgttc
    15601 aagaccgtga aagcggttgg cggcctcgac gtccagctca tctatttcag gggcttcaac
    15661 gagtgccagg cgtcgaaatg ggtcggcgat cccgaggcgc ttgccgggct catgcgcaag
    15721 gttgcttgcg ccggaggttt tacgcagatc ggcaaggtgc tgtcgcacgc gaggcgcgag
    15781 agcgcggcga gcaaggtcaa tgcccttgtc tatgtcggcg actgcatgga ggaggaggtc
    15841 gaccggcttg ccaatctcgc gggtgagctc ggcctgatcg gcgtcccggt gttcctgttc
    15901 caggagggcc acgaccccaa ggccgagcgc gcgttcaggg agatcgcaag gctctcgcgc
    15961 ggcgcctatt gccgcttcga cgcgggctca gcccggcagc tgcgcgagct tctcacggcg
    16021 gtcgccgtct atgcgaccgg cgggcgcaaa gcgttgcaga atttcagcga cgagacgaag
    16081 agcaacgccg cccttcgcct ccttgagcag ctcggctagc gggccgaacc atggcatttt
    16141 ttctcgtcgg ttgcgcgctc ctcgtcctgc ttctccttgc cggacgcggt ctcgctttcg
    16201 caaacaccca cacgctcgcc gtcgcgatcc gcaagatggg cggcgttgcc gcgctcgccg
    16261 ccgcgggttt cctcatcctg cgcggcgcgc tccctctcgc cattccactc gcggtttttg
    16321 gcttttcgct tctgcgccgg agcgccgggt ttgggttcgg cgaccccttc ggttcggcgc
    16381 gcaaagaacc gggccagaag tcacggatca agaccgaccg gctcgaagtg gagctcgatc
    16441 acgacagcgg gcgcatggaa ggcaaatgcc tcgcggggcg tttcgccggc cgttcgcttg
    16501 ccgcgctcag cgatcgcgag gcgatggaac tgctcgccga gctgcaccgg gaaggcgcgc
    16561 cggaagcggc ggtgatggaa gcctatctcg actggcgcct gccggactgg cgcgagcaac
    16621 gctcgaccgc gggcgagaaa agcgaagcgg ggagacggcg gtcaaacggc gcccagcgca
    16681 tgagcgccga ggaggcttac gccgtgctcg gaataggtcc caaggccagc gaagaagaga
    16741 tccggcgcgc gcatcgccgg ctgatgatga agatgcaccc cgaccagggc ggatcgaact
    16801 atctcgcggc gcgtatcaac gaggccaagg aagtgttgct cggccgcctg taaggcagcg
    16861 ccggcgatcg agaatccgcc atgctgcgga gcgcattttc gcgctcgcgg tgaggggcca
    16921 gcggcgaagc cgcgtcaaaa aggattcacc cctcaccctt cccgccattc gccgcgcttc
    16981 gcgcgccggg cgggaagccc tctccctcaa ggggcgaggg aagtaagaaa gacgggcctt
    17041 tcgaggacgt tcggcccgcc tcagggtgat gaaccctact cggcgagcat gaccgcgcag
    17101 tcgagggaga gcttcttgag tgacttgcag gtggctttgg cggcgtcctg cgagaacccg
    17161 gcaaagcgcg cccggtacca ttcctcatcc cccttcatga aggatgcggt aaaggggagg
    17221 tggccgtcga ggaggtcggt ggcccgctgc tggaccatgc cgagcctgtt ctcggcttcg
    17281 gcctgcgacg tgtaggcccc gacctggaca tggtattcgc cgtggtagcg aaacgacttt
    17341 gcggcggtct tgggcgcggc gctgctgacg gtagggacgg ctcgctttgc cggggccggc
    17401 tcggtgatat cgccctgggc aggggccaaa agccgtgccg gggccgatgc cgcagccgcc
    17461 agggccagaa gaggtttctt gatcgccggc agcgagggcg gcggcgctcc gacgaacgac
    17521 gccacgcgct gctccggcgt aggcttggtc tcggaggctt ttgcgaagtg cttgtcgaga
    17581 atgctgcggc aggcggcatc tctttggctc ccggtcctgc ctccaagcac aaccgccaga
    17641 agatgcttgt tcccgcgatg caccgaagcg gcgaggttga agccgcttgc ccgcgtgtac
    17701 ccggtcttga tgccgtcggt gcctttgtaa ccgaacagaa gcttgttgtg attgcggtac
    17761 ttgcggccct tgaaggtgaa atacttcgtt tcgaagacgt tgtaatactg ggggtagtcg
    17821 tggatcagat gcgcggcaag gatggccatg tcgcgcgcgg tggtgatctg ctcctcgttc
    17881 ggcaggcctg aggcattgcg aaaaaccgtc ttggtcatgc cgattcgccg gcccgtgtcg
    17941 gtcatcacgc gggcgaagtt ctcttcgcta ccgccgagat tctcggcgat ggtggcggcg
    18001 gcatcgttgg ccgattgggt caccagcgcc ttggcggcgt cgctgacctt gatcttcgcg
    18061 cctgttttca ggccgagctt ggtgggggct tggcttgccg cgtgaggggt cacgacgagc
    18121 tcggtgtcgg gcgtgatctt gccggcgcgc agatagccga acagcacgta aagcgtcatc
    18181 atcttcgtca gcgaggcggg gtaacgcggc gtctccgcgg cctcggcgtg aaggatggag
    18241 cccgtgttca tgtccacgac gatggaggcc gcgacccgag gcgtggacgc tctcgacttc
    18301 cgcgacgccg cttcggcgcc agatgccacc ataccggcga gcaccagcac gaatgccagc
    18361 gcgagcccag cgccgcgccg cacaggcacc cgacgcctaa ggctcgatga cccacgatag
    18421 gccaagactt cccccgtccg acgaaccatc cctctggcgc acgcggcgcc tcccgcccgg
    18481 ggacgcctcc aaccaattta cccaaccccg tggcaagagg aaggctaaat ttcgccgctt
    18541 gccaaagcgt taaacggttc tttcagggtt attgtgcagt gcaaaaaatt ccggttgatt
    18601 ttggttgtgc aacgcatata taaggcccgt attcgcagca accgcgtcac ccgtgtcgag
    18661 agagggctcc cccatgctca aaggtttcga agagttccag aaggtgggca aggacggctt
    18721 cgatgccgcc gtgcgttcgt tcggtgagat gaacaaaggc ttccaggcca tcgccgcaga
    18781 agtcaccgac tattccaaga aggcgttcga ggattcgacc cgcgccttcg agcagctgat
    18841 cggcgccaag acgatcgagc aggcagtcga gatccagtcc cagtatgcca agaaggctta
    18901 cgacagctac gtcgccgaga tgtccaagct cggtgatatg tatgtcggcc tagccaaaga
    18961 cgcctacaag ccggtcgagg ctgccttcaa caagaagctc gtctgacggc gacgatcagt
    19021 cccaatcttc cccagtaaag agacccgggc tggtcccggg tctctttttt tttgccgctt
    19081 tcctgtcgca actcacccta tgttatggat gtttatggaa gtgcgacccg gcatttgcgg
    19141 ccgcgcgcct tgaaggtaga atggaagagg ggtcgcgagc gagcgctcgc cttggcagat
    19201 gaccaacgag acgccacata gggattgacg atggcgcgga acggcgatcc tcgccggggc
    19261 gacgacaccg gcacccatat catcaccaag acccggccga agaccaagcg gccgagcctg
    19321 tataaggtgc tgctgctcaa cgacgactac acgccgatgg acttcgtcgt atacgtgctt
    19381 gagcggttct tcaacaagcc gcccgaggag gcaacccgca tcatgctgca tgtgcatcaa
    19441 aaaggggtcg gcatctgtgg ggtctacact tatgaggtcg ccgagactaa ggtagcgcag
    19501 gtgacgagct tctcgcgtca gcacgagcat ccgttgcaat gcaccatgga gaaggagtga
    19561 ggatttaggt ggcttcgttt tcgaccagtc ttgagcaggc cctccggcgc gcgctgcaat
    19621 atgccaatga gcgcagccac gaatacgcca cgctcgagca tctgctcctg tcgctgcttg
    19681 acgaccgcga cgctgcggcc gtgatgcgcg cctgcgacgt cgacctcgat gccttgcgcg
    19741 acaagctcac cggctatgtc gacaacgagc tgtcggcgat ggtgctcgat tcccggggcg
    19801 acgcgcagcc gacggccgcg ttccagcgcg tcattcaccg cgccgtggcc catgtgcaat
    19861 cgtcaggccg cgaggaagtg accggggcca acgtgcttgt cgccatcttc gccgagcgcg
    19921 agagccacgc cgccttcttc ctgcaagagc aacacatgac gcgcttcgat gccatcaact
    19981 atatcagcca cggtatcgcc aaacgggccg gcatgtccga gacgcgcacg gtgcggggcg
    20041 tcgatgagga tgccgcgacc gtcgaggccg gcgacgagaa gaagcgcggg gctgatgcac
    20101 tcgaagtcta ttgcatcaat ctcaaccaga aagcccgcgc cggccgcatc gatccgctga
    20161 tcggccgcgg tcccgagatc cagcgcacca tccaggtgct ctgccggcgg cagaagaaca
    20221 acccgctctt cgtcggcgat cccggcgtcg gcaagacggc catcgccgag ggcttggcgc
    20281 gccgcatcgt catgggcgac gtgccggaag tgctttccga ttccaccatc ttccagctcg
    20341 acatgggcgc gctgctcgcc ggcacgcggt accgcggcga tttcgaggag cggctcaagg
    20401 ccgtgatgcg cgagatcgag gcctatcccg gcgccatcat gttcatcgac gagatccata
    20461 cagtgatcgg ggcgggcgcc acttcgggcg gcgccatgga tgcctcgaac ctgctgaagc
    20521 ccgcgctcca gacgggcacg ctccgctgca tcggctcgac cacctataag gagtaccgcc
    20581 agcatttcga gaaggacagg gcgcttgtgc gccggttcca gaagatcgac gtgaacgagc
    20641 cgtcggtgcc ggacgcgatc gagatcttga agggactgaa accctatttc gaggatttcc
    20701 accgcgtgcg ctacaccagc caggcgatca agtccgcggt cgagctgtcg gcgaaataca
    20761 tccacgaccg caagctgccc gacaaggcga tcgacgtcat cgacgagacc ggcgcctcgc
    20821 aaatgctggt gcccgagcgg cagcggcgca agaccatcgg cgtcaaggag gtcgaatcca
    20881 ccatcgccac gatggcccgc attccgccca agacggtgtc gaaatccgac gccgaggtgt
    20941 tgcgcgccat cgaccaggat ctgaagcggg tggtgttcgg acaggatcag gccatcgagg
    21001 ctctggccgc cgccatcaag ctgtcgcgcg ccggcctgcg cgagcccggg aagccgatcg
    21061 gctgctatat gttctccggc ccgaccggcg tcggcaagac cgaggtcgcg cgccagcttg
    21121 cgctcctgct cggcgtcgag ctgctccgct tcgacatgag cgaatatatg gagcgccaca
    21181 cggtgtcacg gctgatcggc gcgccgcccg gctacgtcgg cttcgatcag ggcgggctgc
    21241 tcaccgacgg cgtcgatcag catccgcatt gcgtgctctt gctcgacgag atcgagaagg
    21301 cgcatccgga cctgttcaac attctgttgc aggtcatgga tcacggcaag ctcaccgacc
    21361 ataacggcaa gcgcgtcgac ttccagaacg tcatcctcat catgacgacc aatgccgggg
    21421 cgagcgacct cgccaaggcg gcgatcggct tcaaccgcac gaagcgcgag ggcgacgacg
    21481 aggaggcgat caaccgcatg ttctcgccgg agttccgcaa ccggctcgac gcggtcatcg
    21541 ccttcgccag tctcacgccg gaggtgatct ccaaggtggt ggagaagttc gtgttccagc
    21601 tcgaggctca gctcggcgac cgcaacatca ccatcgagct gacgccggag gccaacaaat
    21661 ggcttgccga gcggggctat gacgacaagt tcggcgcccg gccgcttgcc cgcgtcattc
    21721 aggagcagat caagaagccg ctggccgaag agattctgtt cggcgcgctg aaggatggcg
    21781 gcatcgtgcg ggtcctcgtg gaggaaaagg ggccggagaa ggcgctcggc ttcgaatatc
    21841 tcaagcccga tacgccggtc aggccgcgcg atcccgagga aaacgaggac gaagggccga
    21901 cggatggcga gcccaaggga ccgccgaaat cgggcgctcc gaagccgcgc gccaaacgcg
    21961 gcgcgcgcaa gcccaaaggc ggccgcgacc ggagcgtcgg cgcgagcctc gtgcccaagg
    22021 tccccctcgg ctcgaaatag ccgcgctgtc actcccaacg ccctcatgct gaggagcgcc
    22081 cggcggctcg ccaagggcgc gcctcgaagc acgcacacaa tttctcgctg ccgcggttct
    22141 cttttctaac ctccccctcg tggggaggtc cggcggcgcg cgaagcacaa cgtggctacg
    22201 cgggacgctg gggtggggcc tgcgcgggca tgacatttgt ggtggtgtaa gattacccct
    22261 tcagtgtctt ccggattttt tccgcggtgg cggcgagcgc cgtctggtcc cccatt
//
LOCUS       DASUQP010000005        26336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1773830, whole genome shotgun sequence.
ACCESSION   DASUQP010000005 DASUQP010000000
VERSION     DASUQP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 26336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26336
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773830"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VFY74_00170"
     CDS             complement(<1..1930)
                     /locus_tag="VFY74_00170"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5930158.1"
                     /translation="MAKSSTGLGPRLLYALIVGAVIIASLALRVWDPSPVARLRALVF
                     DTYQRMDPRAFDPALPVRVVDIDEDSLKAIGQWPWPRTVLADLVDKLAAGGAAAIGFD
                     IVFPEPDRMSPANALRFWPKSEAVQGLREEIEKLPSNDQVFAEAIGKAPVALGFIAAP
                     QGRSIPEIKAGFAHGGDDPKLFAPYYPGAAASLKELQDRAQGSGSLNWIPEHDQIIRR
                     MPMVIRVGDTLYPSFAADMLRLAQGASTYVVKSSGASGEKAFGERTGIVKIRIGDFEV
                     PTEANGQMWIRFTPENKARYLPAWKVLNGEIPPDDIAGRILVIGTSAAGLLDLRATPL
                     EASVPGVSLHAQAIEQVLQGAFLQRPDFATAAELFYILALGALIAFFIYRLGALGSAV
                     LCGAAIAAVIGVSWYAFEAFGWLVDPIYPAVALAAIYLAGTLVVFLRTERERNRVRNA
                     FSHYMAPALVERLADDPSRLKLGGETRDMTLLFSDVRGFTTISEGLDAEELTRFLNSL
                     FTPLSNIILEEQGTIDKFMGDALMAFWNAPLEDSAHPSHACSAALRMMGAMGRLNESW
                     RDEAEAKGRKFKPVQIGIGLNTGVCCVGNLGSETRFDYSVIGDNVNVASRLEGQSKTY
                     DVGTVVGESTTARAPDFAF"
     gene            2148..2675
                     /locus_tag="VFY74_00175"
     CDS             2148..2675
                     /locus_tag="VFY74_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930159.1"
                     /translation="MMRLPLVFVVAMAGLGLLGGGVEAARMWMPKTTNPYCDIDTYVL
                     RDLPEQAMSIRDSSGKPVIVVSGFTLADKPSYTQFLLAHECCHHTLGHMDRFSEQLGQ
                     VGPQRFFFIAPALKQMELDADCCAVKMLRSRNETGGIEAAEEAMIVFGTAQTGAHYPT
                     GNERVENMAKCAAQD"
     gene            2778..3647
                     /locus_tag="VFY74_00180"
     CDS             2778..3647
                     /locus_tag="VFY74_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HEX5930160.1"
                     /translation="MVITKRRRSPAWSLAVALACAALAAILGAAPIKSAEPAASGLLR
                     VCADPNNMPFSNLEGEGFENKLAELIAQKLDARLEYSWFAEASGYVPNTMGRGACDLV
                     MGYAQGTGLIEDTNPYYYTSYLLIYRQDDSALGGVESLSDPRLKGKRIGFVARTPPSS
                     VLAMHGLVASAKPFEIHSGDSATKAAVTMISEIAAGTLDAGILWGPVGGYYAQRADVP
                     LTLVPLVKERAGPRTIYGITMGVRPKDPQWKHRINKFIAANQGEINVILQSYNVPVLD
                     DKGNVVAAASAER"
     gene            complement(3667..4962)
                     /locus_tag="VFY74_00185"
     CDS             complement(3667..4962)
                     /locus_tag="VFY74_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5930161.1"
                     /translation="MFGVAAALLLAAGAALTGAWSVRAEEPPAPAAKSDVPKDRDGGF
                     VVQVPQSPPLTPLPIGYLRESVDHPRPASRLDVEPKDAGIAGAKMAVEENNAGGRFTG
                     HHYSLDVSTVSSAEKAVEALQKFLDSGHHYIVVDASADTLLKLSDWAKGKDILLFNIG
                     ATDVSLRQENCRANVMHVVPDRYMLADALAQYLVTMKWTNWLLVHGSTPGDLAYADAI
                     KRAAGRFGATIVDTREYKDISGGRRDDVGAVPPGKQASLDAAFAAAPDYHVIVVADEN
                     QVFGPYIPYRGGADARPVAGTTGLIATTWSPGHEKWGATQANNNFEKDFNRLMLPIDY
                     QAYVAARTIGEAVTRNPGSDFATVAAFVRGPELQLAPFKGIKQQFRPWDGQFRQPLII
                     ATDKVPVSFSPQRGFPHASHPEIEVDTLGIDEPDSLCKM"
     gene            5300..6016
                     /locus_tag="VFY74_00190"
     CDS             5300..6016
                     /locus_tag="VFY74_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930162.1"
                     /translation="MGFSVRSALRFGWETFKKRPWFFVLAMLLIVIAQGLVQGIGRAS
                     DASLAGAAEELGFLASLVHLALSTLVSMGVTAFALAAHDNPDTVELSALWHPHPFWKY
                     LGLSIIFALVAVAAFLLGLALVAVLGLETGLAIGIPLLLVLGAIFALMFMFSGFLVID
                     RELGPIEAMKGSYRMTYGYKWSLLGLLLMLLLINVLGLLALIVGLFVSAPVSLLAATH
                     AYRVLGAGAGTRPADAALAA"
     gene            6260..6868
                     /locus_tag="VFY74_00195"
     CDS             6260..6868
                     /locus_tag="VFY74_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930163.1"
                     /translation="MSSSAPISRSALSLARLPQWAGRRFSSPLMPTRIKSSLPRYGTP
                     RPYWKYLGAGILLLLVMLAGFLFATLLLVGVNIPLGLIAIVVAVVASVIFVLMFMFTL
                     VIVIDRELGPIAAMKESHRITRGHKWRLAGLMLVFMLISLLWQGFNKVLDAPMPINLL
                     GALAIVAALLVLSPVASLAVTRAYRVLSGAAGIQPADAALAA"
     gene            complement(6922..8145)
                     /gene="pqqE"
                     /locus_tag="VFY74_00200"
     CDS             complement(6922..8145)
                     /gene="pqqE"
                     /locus_tag="VFY74_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEX5930164.1"
                     /translation="MSEEFRGNGHCETLVVDDEPTEGVSGSSSEALCARAPVGLLAEL
                     THRCPLQCSYCSNPIELERVNKELSTEMWCSVMSQAGAMGILQVHLSGGEPTARKDLE
                     QIVSAASEAGLYSNLITAAVTLNRARLEDLAKRGLDHVQISFQDVDAENAERIGAYPG
                     ATQKKLDVARWVTELGLPLTINAPIHRHNIHNVGRYIELAVKLGAQRLEIAHVQYYGW
                     AYLNRAALIPTYDETLKSIELVERERERLKGVLTIDMVVPDYYAKRPKPCMGGWGKGF
                     MNITPAGKVLPCHAAETIKTLRFDNVQDRPLLDIWLNSDAFNAFRGTDWMKEPCRSCA
                     FREIDFGGCRCQAMAITGDARNTDPACALSPYHGEMVRLAREEASRPPAPFVYRNPRN
                     AAEAAKAERPLVPAE"
     gene            complement(8142..8444)
                     /gene="pqqD"
                     /locus_tag="VFY74_00205"
     CDS             complement(8142..8444)
                     /gene="pqqD"
                     /locus_tag="VFY74_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEX5930165.1"
                     /translation="MSDTTSPARLIVGGETRPHLPAYLKLRHDAGRDRWILLAPERVL
                     TPDQTAVAVLKLCDGKRTVNEIVAALAKEYSAPAEVIRADVLDLLQGLADKGYIKG"
     gene            complement(8441..9175)
                     /gene="pqqC"
                     /locus_tag="VFY74_00210"
     CDS             complement(8441..9175)
                     /gene="pqqC"
                     /locus_tag="VFY74_00210"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEX5930166.1"
                     /translation="MTKIMSPEELEQALRAIGAERYHDKHPFHKLLHGGKLNKGQVQA
                     WALNRYCYQSAVPRKDAALISRCHDRQLRRDWIHRIMDHDGFGQEEGGIERWLVLTDG
                     LGLDRDYVISMKGALPETRFAVEAYVNFVREKTLVEAVASSLTELFAPKIHKERISGM
                     LENYDFISDEVMAYFKRRLTQAPDDANFALEYVKRNARTPEAQAAALDALRFKCNVLW
                     AQLDALHYAYVEPGLVPPGAFVPGKA"
     gene            9552..11069
                     /locus_tag="VFY74_00215"
     CDS             9552..11069
                     /locus_tag="VFY74_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930167.1"
                     /translation="MALRRPISDFYVAGRLVPPIFNGAAIAVGLLPVIVFPGLAGALV
                     QGSEGASLLAIGGGAGLVAIGFFIAPYLRRFGGYTVPDFLAERFDLPGIRPLAVLAVI
                     LCTFPALALALLVLGTLVTRVFAIDLGTGVVLGVTMLLACSFAAGMRSASLTQIVQCA
                     ILLAASLVVLALLLWQGGGFFPALGGAGLRAALAAIQLETFTAEDTLNRFALLFCLAA
                     GIAALPHLLVRSLVTPSIEGARMSFLWALPFTAALCILAAPSLALFGEVPAAADNMPA
                     LFRLGFAVTGAIAALLALGSGLVLALANTLSYDLYYKMVHPTASTERRIFVARGAILA
                     VALLAAWAALAQPEPTRAMTGAVFSLAASAFLPALVLGIWWKRASGEGALAGMLTGLA
                     VCLYYMLAPRYFPFAFYETSSFLSDATVAEAQRYNDLRQSYYLADAATRGVALTAWED
                     AARAVANWGGVKRDFAGLFAVPIGLLVMIGVSLFTPAPAREIQSFVEDLRKTSAA"
     gene            complement(11085..11687)
                     /locus_tag="VFY74_00220"
     CDS             complement(11085..11687)
                     /locus_tag="VFY74_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930168.1"
                     /translation="MSIQSAALAVLLLTATAATAFAEDQPAQQATAAVPQWTEFQSPE
                     RGFAVTFPGSPRSTSAPVEGQNPLLQYDFQVSVGEDTVYSVVVFEYPPGKGPSPDNDT
                     YVKLVNAYAKGSESRLRKRGAATIAGYPGFEAISDDKKGKLSHLIDVVPSGDRIYMLV
                     TAGPKSHATGDEATRFRDSFRLLGQSEPPPAQSAATPSDD"
     gene            complement(11768..13357)
                     /locus_tag="VFY74_00225"
     CDS             complement(11768..13357)
                     /locus_tag="VFY74_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX5930169.1"
                     /translation="MTEPLKRLVRLLAPVTLAALLFFPAPTMGEVADARPHAATSGQE
                     SSGDQSVGTQPAKADAPVPPASITQHSIHLAGVEVPYSAKAATLPLRDAQGKTLADIF
                     YVAYVREPQDTKRPITFVFNGGPGAASAYLHLGAIGPKSVTVSDKGELLGPPPSLVAN
                     DSSWLDFTDLVFVDPVATGYSRARDGKDESDFFGVEEDTRALANFIRLYLTDAGRLVS
                     PVFLAGESYGGFRVATITRALQKTGGISPNGLILVSPALEFALLNGEDYDPLPWALGL
                     PSFAAVNFESKGVTGREALAEAVKEAERYALTDYLVALASGAEEGAGRASGTVAELTG
                     LPVETVRRTFARIPPSLFIKEFDRARGQVLSRYDGSVSGPDPNPASSWPRGPDPVLDA
                     TVPLWTGAFVQYAQGELGYKTDMSYRLLNRDVRGKWDFGTRPTRQGYAGVLDDIQEAR
                     AANRGLEVFIATGYTDLITPYSTPVYLVNQLAPLEGASPIVVETYAGGHMLYLRPETR
                     PALKADVEAMYERALKSSPQG"
     gene            13897..15525
                     /locus_tag="VFY74_00230"
     CDS             13897..15525
                     /locus_tag="VFY74_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930170.1"
                     /translation="MREKRTWPPDRDSDGETAAAFEAAVAVVKESVVRSGEVLSSARE
                     DLSDHQRWLKAQAAAVESDRERLARWLQRQRERQEAIERREQARARRRARRQAAIGSV
                     RDAIAGAVFAVRAAVWGSISSIMGGLNAIDATAARGLRWIGASIRDAALYVAAAFRRA
                     ALSMGRAFRGIALGTVGALRRAVLWSRRAFGGAFHFGFSSVASTLGSVRALLASGVAR
                     AGSKLQVVAPAAGHAVTRGFGLIAASTHKASDTIGPRVRENFVWLSGRAENLRRATGE
                     AVAPALSRVAVKTRALAPTLSARMAEARTVVGSHLRDAAHGARGLLATSKTSPEAAEN
                     AVSFRQQIGRLDLSQMLIIAGALLLVCGTLMLGGGLLLRAGKPAVAAASTSEPIAWLF
                     EHDTLELDERSVFAFATTPEGVRVRGFAIGGVNMSEQILDAVGAVIKPDREVKDVKLA
                     MRVVAPEEQAGETKIFEPGVPGTIPPQAQFALQFAFPGDGGMTPDQVLSTYGGVMLKV
                     RYEVAGTEKSFIHYLSPSFLERQLAEIMAGSKGS"
     gene            complement(15569..15877)
                     /locus_tag="VFY74_00235"
     CDS             complement(15569..15877)
                     /locus_tag="VFY74_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930171.1"
                     /translation="MTRFVLLSLALVVCLAASGARAQTTTEQEAPFQSVRPSQRPCGL
                     GSQSVRVCNDDFRSCNSICFAETLDASFDFTGCGLRCCNNFKACLSIRGCATLTLNCF
                     "
     gene            16026..16307
                     /locus_tag="VFY74_00240"
     CDS             16026..16307
                     /locus_tag="VFY74_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930172.1"
                     /translation="MRFTLAVLGLLIPLAAVLGCATQNEASRAAEQAVADSEDEATCQ
                     EKSGDEPKAYKACREAMSDARAQQAAIQEQKRRDFDRVLGAGTDGLNNY"
     gene            complement(16563..16754)
                     /locus_tag="VFY74_00245"
     CDS             complement(16563..16754)
                     /locus_tag="VFY74_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930173.1"
                     /translation="MPRSYLGQTTADILHTTTVGPGTQLLDVCVTVPPRAAAFTARLA
                     CARVDAVAMSRPFPFALTG"
     gene            16924..17190
                     /locus_tag="VFY74_00250"
     CDS             16924..17190
                     /locus_tag="VFY74_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930174.1"
                     /translation="MNATSSILDPFTLAGIAGTALIIVAYFANQQGWLKADDWKYPLA
                     NLIGAVLILVSLITAWNLPVAVIESFWAAISIYGLIKNAGRSKG"
     gene            complement(17187..17885)
                     /locus_tag="VFY74_00255"
     CDS             complement(17187..17885)
                     /locus_tag="VFY74_00255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX5930175.1"
                     /translation="MKPILILRNDQHEGAGALATLIDVRGLEQRSVLGWEADYARLPS
                     DAFSALVVLGGAQSAYETDQYPYLSGEMQLCRDFIGEGKPVAGFCLGAQLLACAVGGE
                     VVPGKRKEIGWYDLRLTEAGANDPLMEGHPRKLLSYHFHGDIIAKVPGAERLAYSDMT
                     ECQLFRYGNSAYGFQYHAEADRPLLEIMCRNNRDYMAANGFDAEAIIAESATRLPAFE
                     RHCRIMLERWLDLI"
     gene            18068..18997
                     /gene="ftcD"
                     /locus_tag="VFY74_00260"
     CDS             18068..18997
                     /gene="ftcD"
                     /locus_tag="VFY74_00260"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX5930176.1"
                     /translation="MLSDPNFSDGTRREVIEQIVDPFRGRPGVKLIGYEPDPDFDRLP
                     IEALGRLEPMKEALLAAAGKAYELIDMEKQRGAHPRIGAVDTIEIYPAKDITIEECRD
                     FAEELGNELFKRYAVPVYFTGKNARRPENEGLTFIRKGNYEGLKEAVLKDPSRAPDLG
                     PARLHPTAGATIVGAVDEHDAYFNVVLDTTDLSIAKKLASYVRGKTGGFTNIQGVIGL
                     PQTHRRTGKACVAVSCEISNPLKTPIHRVFNLLRSEAARYGVNVIAGQICGTISAEVL
                     VQTAEWYLMLEGINGPWDYKSQILENHLLELES"
     gene            19248..19697
                     /locus_tag="VFY74_00265"
     CDS             19248..19697
                     /locus_tag="VFY74_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5930177.1"
                     /translation="MAKPSQPRRPRALIVEDEILIALGLEADLNELGFDACGLAANAR
                     QALSFAMEDMPDLAVVDVYLDGVRDGIETAKTLRDFCGVPIVFVTAYGHEQGIIERIQ
                     RQVPGAPILSKPLYGSRLAEAIAEVVKIAPSRRAQRAAAHLHAPAVH"
     gene            19996..21468
                     /locus_tag="VFY74_00270"
     CDS             19996..21468
                     /locus_tag="VFY74_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5930178.1"
                     /translation="MLSTDELLRRPSRPPDHAAENQALIDLMQALATSPEVILQRLAD
                     TALKLCRAHSAGLSLLEDTDQKSNFHWRAIAGQWAPHLNGGTPRNFGPCGTVLDRNAA
                     MVCSHPELDFPYFAPVKPLLEEALLIPFYVKGEAVGTIWIVAHDTSRRFDAEDLRVMT
                     NLGAFAAAAYQMWLSLNATQRIVAIVESSDDAIVSKDLNGIISTWNQAAERIFGYAAK
                     EVIGKPITILIPPERIDEEGKILERIRSGGRVETYQTVRLRKDGSPVDISLTVSPLRT
                     AEGQVVGASKIARDITEHKRSEQQIAILMGEVEHRARNVLATVQATVHLSQADTPDGL
                     KRAIEGRIQALANVHKLFVESRWSGAGLEVLVKDELAAYGVDGERRAHIEGPPVLLRP
                     DRAQATAIALHELATNAAKYGALSAGGGRVHVAWSLNPDGGFVLRWNESGGPLVEPPM
                     GKGFGMRVMERIIRDQVKGEIRFDWRREGLVCEIALPAAR"
     gene            21620..23386
                     /locus_tag="VFY74_00275"
     CDS             21620..23386
                     /locus_tag="VFY74_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX5930179.1"
                     /translation="MAETVADFMLSRLSQWGVRRIYGYPGDGINGILGALDRALDRFQ
                     FVQVRHEEEAAFMATAHAKFTNEVGVCLATSGPGAIHLLNGLYDAKLDHQPVLAIVGQ
                     SVRAALGGAFQQEVDLHSLFKDVAGNYVQTCTAPVQMRHLVDRAMRIAHAERCVTCLI
                     VPNDVQDLKAEEPPHEHGTIHSGIGYSAPCMVPAEAELRRAAEVLNEGEKVAILVGAG
                     ALQATDEVIKIAEMLGAGVAKALLGRAALPDDLGFVTGQIGLLGTRPSWDMMQDCDTL
                     LMVGSRFPYAEFLPKEGNARGVQIDIDPKVLSIRYPMEVNLVGDAAATLNALLPMLER
                     KRDRSWRNALETKVAEWWKEEEERAHLDGDPLNPELVFWEASPRLPDKAIIAADSGTS
                     ASWFARALKLRRGMKASLSGTLATMCCAIPYATAAKFCFPDRVAIAFVGDGAMQMLGM
                     NGLITIAKYWREWEDPRLIVVVLNNRDLNMVTWELRAMGGTPKIEETQNLPDFQYAQL
                     AEMLDLKGVRISAAEQVVPAWEAALAADRPFVIDAVVDPTIFAVPPHVTFEQTKNYLA
                     AILKGDREAAWTMWRSLKQMVS"
     gene            23485..23763
                     /locus_tag="VFY74_00280"
     CDS             23485..23763
                     /locus_tag="VFY74_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930180.1"
                     /translation="MCEIIKWTFAAAYAVALFLLAAGSWGWPGADRDPLSALLLIALG
                     LPWNRFIDYFGGTSPAVLAMLAPALNLAILFSICSTLRRRRNRAPGAP"
     gene            complement(24185..24259)
                     /locus_tag="VFY74_00285"
     tRNA            complement(24185..24259)
                     /locus_tag="VFY74_00285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24225..24227),aa:Thr,seq:tgt)
     gene            complement(24394..24816)
                     /locus_tag="VFY74_00290"
     CDS             complement(24394..24816)
                     /locus_tag="VFY74_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930181.1"
                     /translation="MLRSALALISSLQIGLRIRESIERSLRQALFVAVAALLLIAAAC
                     FGLFAAYQALILSYGFTPPQAACLIAALLAALALVVLAVFFLTARRPKPTASSPFASA
                     GDSVGLLDQSVGKAMQQVGPVGLLAIAFIAGLLASRRR"
     gene            complement(24829..25095)
                     /locus_tag="VFY74_00295"
     CDS             complement(24829..25095)
                     /locus_tag="VFY74_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HEX5930182.1"
                     /translation="MSSIDSDRRRGPGDRDADELANQIEAIRADLQNLTSTVGRIAGK
                     QVNRVQDKAIATATEAEEAIRRNPLQAVAIAVGLGFLFGVFTRR"
     gene            25453..25680
                     /locus_tag="VFY74_00300"
     CDS             25453..25680
                     /locus_tag="VFY74_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930183.1"
                     /translation="MKGRSSFTESAAPIQERVLLHHRRRVQSFYCYPRNYWWFYRPYT
                     TALDGHPRCMPYFHYLGPYDRRGVGPDRYVK"
     gene            complement(25918..>26336)
                     /locus_tag="VFY74_00305"
     CDS             complement(25918..>26336)
                     /locus_tag="VFY74_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930184.1"
                     /translation="AAAAEFPKTGSAEYDTYYVENPVAKIDSGAGTGTIVDSTGINRN
                     VKGDGPFHDMSVRCLYHQSSVGETFHFNGSCVETDKDGDNLFTTFDDKNHYLMGGTGK
                     YKGITGTVPYTVVQLHDTVGGRTAYIVNHKATWEIK"
BASE COUNT         4586 a         8120 c         8542 g         5088 t
ORIGIN      
        1 gaaaggcgaa gtcgggtgcc cgcgccgtag tggactcgcc gaccacggtg ccgacgtcat
       61 aggtcttcga ctgtccttcg agccgcgagg cgacgttgac gttatcgccg atcaccgaat
      121 agtcgaaacg cgtctccgag ccgagattgc cgacgcagca gacgccggtg ttcaacccga
      181 tcccgatctg gaccggcttg aacttccggc ccttggcttc ggcctcgtca cgccagcttt
      241 cgttcaggcg ccccatcgcg cccatcatgc ggagggctgc actacaggcg tggctcggat
      301 gggcgctgtc ttcgagcggc gcgttccaga aagccatcag cgcgtcgccc atgaacttgt
      361 cgatggtgcc ctgttcttcg aggatgatat tgctcaaggg cgtgaacaga gagttgagga
      421 agcgcgtgag ctcctcggcg tcgaggccct ccgagatggt ggtgaagccg cgcacgtcgc
      481 tgaagagcag cgtcatgtcg cgcgtctccc cgccgagctt cagccgcgag gggtcgtcgg
      541 ccaaccgttc gacgagggcg ggcgccatgt aatgagaaaa ggcgttgcgc acccgattac
      601 gctcgcgctc ggtgcgcaag aacacgacca gcgtgccagc gagatagatc gcggcgagcg
      661 cgaccgccgg gtagatggga tcgaccagcc agccgaacgc ctcgaaagcg taccaggaca
      721 cgccgatcac ggccgcgatt gccgcgccgc aaagaacggc gctgccgagc gcgcccaggc
      781 gatagatgaa aaaggcgatg agcgcgccga gcgcgaggat gtagaagagc tcggcggcgg
      841 tggcgaaatc gggtcgttgc aggaaggccc cttgcaagac ttgctcgatg gcctgggcgt
      901 gaagggagac gccgggaacg gaagcctcga gcggcgtcgc ccggagatcg agcaggcctg
      961 ccgcgctcgt gccgatgacc aggatgcggc ccgcgatatc atcggggggg atctcgccgt
     1021 tcagcacctt ccaggccggc aggtaacgcg ccttgttttc gggagtgaaa cgaatccaca
     1081 tctggccgtt cgcctcggtc ggcacctcga aatcgccgat gcgaatcttg acgatgccgg
     1141 tcctctcgcc gaacgccttc tcgccgctcg ccccggacga cttcaccaca taggtcgagg
     1201 cgccctgggc gagccgcagc atatcggcgg cgaaggaggg gtagagcgta tcgcccacgc
     1261 ggatcaccat gggcatgcgg cggatgatct ggtcgtgctc gggaatccag ttgagcgatc
     1321 ctgacccttg cgctctgtct tgcagctctt tcaggcttgc cgcggcgccg ggataatacg
     1381 gcgcgaacag tttcggatcg tcgccgccat gggcgaagcc cgctttgatc tccgggatgg
     1441 acctgccttg cggcgcggcg atgaagccga gggcgaccgg agccttgccg atcgcctcgg
     1501 caaagacctg gtcgtttgac ggcagcttct cgatctcctc gcgcaagcct tgcacggcct
     1561 cggatttcgg ccagaagcgc agggcattgg cgggcgacat ccgatcgggc tcgggaaaca
     1621 cgatatcgaa gccgatggcc gcggcgccgc ccgccgcgag cttgtcgacg agatcggcaa
     1681 gcaccgttcg cggccaaggc cactggccga tggccttcag agagtcctca tcgatatcga
     1741 cgacgcgcac gggaagcgct ggatcgaagg cgcgcgggtc catgcgctga taggtgtcga
     1801 aaacgagtgc ccgcagcctg gcgaccgggc tcgggtccca gacgcgcagg gcgagcgagg
     1861 cgatgatgac cgcccccacg atgagggcat agaggaggcg cgggcccaat ccggtgctcg
     1921 actttgccat gtgggtgcgc gccggaccgg cgcgagatgg cagaccctag ccaatggcgc
     1981 tccgccgctc cagcgcgaag ttccgggcct ctgtcacgcg gcgggtgttg cccttcacat
     2041 ccgcgtgtcc tgcgtccatt tgccggtgac gacccgcctc cggctcaact aacgtaccgt
     2101 gccgggaacg ggtaagcctc gcttgcggcc ttggctttgg tggtcggatg atgcgtcttc
     2161 cactcgtctt tgttgtggcc atggcaggtc tcggcctgct cggcggtggc gtggaggcgg
     2221 cgcgaatgtg gatgccgaag accacgaacc cttattgcga tatcgacact tacgttctcc
     2281 gcgatcttcc cgagcaggcg atgtccatcc gcgactcgag cggcaagccc gtaatcgtgg
     2341 tgagcggatt taccctggcc gacaagccgt cctacaccca gttcctgctt gcgcatgaat
     2401 gctgccatca tacgctcggc catatggatc gctttagcga acagctcggt caggtcggtc
     2461 cgcagcgttt cttctttatc gcgccggccc tgaagcagat ggagctcgac gccgattgct
     2521 gcgccgtcaa gatgctgcga tcgaggaacg agacgggcgg catcgaagcg gcggaagagg
     2581 cgatgatagt cttcggtacc gcccagaccg gcgcgcatta tccgacggga aacgagcggg
     2641 tcgaaaacat ggccaaatgc gccgcgcaag actgatgcgg tcgcgcctct cggcccgttt
     2701 tgtcagcgaa gtttaatgat gctcggactg gcggcgcctc gggccgattg ccatattgtg
     2761 ccgctgtgag cagcccgatg gtcataacga agcgacggcg gagcccggct tggtctcttg
     2821 ccgtggccct tgcctgcgcc gccctcgccg ccattctcgg tgcggcaccg atcaagagcg
     2881 ccgaaccggc cgccagcggc ttactgcgtg tctgcgccga tcccaacaac atgccgttct
     2941 cgaacctcga aggcgaaggc ttcgagaaca agctcgcgga gctgatcgct cagaaactcg
     3001 atgcccgatt ggaatattcc tggtttgcgg aggcgagcgg ctacgttccc aatacgatgg
     3061 gacgcggggc ctgcgatctc gtcatgggct acgcgcaagg gaccggcctg atcgaggaca
     3121 ccaatcccta ttactacacg tcctatcttc tgatttatcg ccaggacgat tccgctcttg
     3181 gaggtgtcga aagcctttcc gacccgcgcc tcaagggcaa gaggatcggc ttcgtcgcgc
     3241 ggacgccgcc gtcgagcgtg cttgccatgc atgggcttgt cgcgagcgcg aagcctttcg
     3301 agatccactc cggcgacagc gccacgaagg ccgccgtaac gatgatttca gagatcgccg
     3361 ccggcacgct tgacgcggga attctgtggg gcccggtcgg cggctactac gcgcagcgcg
     3421 ccgacgtgcc gctgacgctc gttccgctgg tgaaggagag ggccggcccg cgcacgatct
     3481 acggcatcac catgggcgtg cgcccgaaag acccgcagtg gaagcacagg atcaacaagt
     3541 tcatcgccgc gaaccagggc gagatcaacg tgattttgca gagctacaac gtgcccgtgc
     3601 ttgacgataa gggcaatgtg gtcgcggccg cgtcggcgga acgctgaggg gacgcgcccc
     3661 tcgcgttcac atcttgcaca gactgtcagg ctcgtcgatg ccgagcgtgt cgacctcgat
     3721 ctcggggtgg cttgcatgcg ggaagccgcg ctgcggcgag aaggagacgg gaaccttgtc
     3781 ggtggcaatg ataagcggct gccggaactg cccgtcccac ggacggaatt gctgcttgat
     3841 gcccttgaac ggcgcaagct ggagctcggg gccgcgcacg aaggctgcga ccgtggcaaa
     3901 atcggagccg ggattgcggg tcaccgcctc gccgatggtg cgcgccgcca cgtaggcttg
     3961 atagtcgatc ggcaacatca ggcgattgaa gtccttctcg aaattgttgt tggcctgggt
     4021 cgcgccccat ttttcgtggc cggggctcca ggtcgtagcg ataagcccgg tcgtgcccgc
     4081 gaccgggcgg gcatccgcgc cgccgcgata gggaatgtag ggaccgaaga cctggttctc
     4141 gtcggccacg acgatcacgt gatagtcggg cgcggcggcg aatgccgcat cgaggcttgc
     4201 ctgcttgccg ggcgggaccg cgccgacgtc gtcgcgcctg ccgcccgaaa tgtccttgta
     4261 ctcgcgcgta tcgacaatgg tggcgccgaa gcggccggct gcgcgcttga tcgcgtcggc
     4321 ataggcaaga tcgcccgggg ttgagccgtg gacgagcagc caattcgtcc atttcatcgt
     4381 gacgagatat tgggctagcg catcggccag catgtatcgg tcaggcacga catgcatgac
     4441 attggcgcgg cagttctctt gccgcagcga gacgtcggtg gcgccaatat tgaacagaag
     4501 gatgtccttg cccttcgccc agtcagagag cttgagcagc gtatcggcgg acgcatcgac
     4561 gacgatatag tgatgcccgc tgtcgaggaa tttctgcagc gcctcgacgg ccttctcagc
     4621 ggacgagaca gtgctgacat cgaggctgta gtgatggcct gtgaagcgac cgccggcatt
     4681 gttctcctcg accgccatct tggctccggc aatgcccgcg tctttcggct cgacgtcgag
     4741 ccgcgaggcg gggcggggat gatcgacgct ctcgcggaga taaccgatcg ggagcggggt
     4801 caagggaggc gattgcggaa cttgaaccac gaagccgccg tccctgtcct tggggacatc
     4861 gctcttggcg gcaggggcgg ggggctcctc ggcacgcacg gaccaggcgc ccgtcaatgc
     4921 ggcgccggcc gcgagcagaa gcgcggcggc aacaccgaac aacggttcgc gccgcctcaa
     4981 tccgcggcag ggaaacttgg acgaaagatg catgggttct agcaccgaat gacgcgagca
     5041 cctgcaccgg ttgtcggttt caaagtctgc gcttggcgag ggggcccgct ccacaggccg
     5101 agcggctttc aagcatggta gcgataaaga aagcggcatt gcaaggcgga aacccggacg
     5161 ggataatttg gtgccacagg gattgccttc gctttgcagg cctcgctcag aggctcaaga
     5221 gcgatccggg cctgcctgcc ttgtgggaaa ttctgagcgc tcttcccgtt aactctgccc
     5281 cgcatgctat cctgcgccga tgggattctc tgtcagatcg gcgcttcgct ttggttggga
     5341 gaccttcaag aagcgcccct ggttctttgt cctcgcgatg ctcctcatcg tcattgctca
     5401 gggcctggtc caggggatcg ggcgtgcgag cgacgcgtcg ttggccgggg ctgcggagga
     5461 gttgggattt ctcgccagcc tggttcatct cgcgctcagc acgcttgtca gcatgggcgt
     5521 tacggccttc gcgctcgcgg cgcacgacaa tcccgatacg gtcgagcttt ccgcgctttg
     5581 gcatccgcac ccgttctgga aatatctcgg tctgtcgata atttttgcgc ttgttgccgt
     5641 ggccgccttc ctgctgggcc tcgctctcgt tgccgtgctc ggtctcgaga ccggtcttgc
     5701 catcggcatt cctcttctcc tcgtcctcgg cgcgatcttc gcgctgatgt ttatgttttc
     5761 ggggttcctc gtgatcgacc gcgagcttgg tccgatcgag gcaatgaagg gcagctaccg
     5821 catgacttac ggctacaaat ggagcctcct cggactgctc ctgatgctgc tcctcatcaa
     5881 cgtgctcggc cttcttgctc ttattgtcgg ccttttcgta tcggcgccgg tctccttgct
     5941 ggccgcgacg catgcctatc gcgtcttggg tgccggcgca gggacgcggc cggcggatgc
     6001 ggcgctggct gcttgacgca gcgccatgcg cattgtgtga aattattggg gtttgtcccc
     6061 taacttggct tgcgtgctat cctgccgcct atgggattct ccgtcagctc cgcgcttcgc
     6121 ttcggttggg agaccttcaa gaagcgcccc tggttcttta tcctcgcgac cctcgtcgtg
     6181 gggcttgttg agcttctcat ggaactggtc gcgggcgcga tcgctatcct cttcgcaagc
     6241 tccgagaagg aattgctgat tgtcttcttc agcgcctatt tcgcgctcgg ccttgtcttt
     6301 ggcacgcttg ccgcaatggg ctggacggcg tttttcctcg ccgctcatgc caacccggat
     6361 aaagtcaagc ttaccgcgct atggcacccc ccgcccgtac tggaaatatc tcggtgcagg
     6421 cattctgctt cttcttgtta tgctggctgg ctttctattc gcgactttgc tcttggtggg
     6481 cgtcaacata cccctcgggt taatcgccat tgtcgtcgcg gtcgtggcct ccgtgatctt
     6541 tgtgctgatg ttcatgttta ccctcgtgat cgtcatcgac cgggagctcg gtccgatcgc
     6601 agccatgaag gagagccacc gcatcacacg aggccataaa tggcgtctgg cgggtctgat
     6661 gctcgtcttc atgctcatca gtctgctgtg gcaagggttt aataaggtgc tcgatgctcc
     6721 catgccgatc aacttgctcg gagctctggc tatcgttgcg gcccttctcg tcttgtcgcc
     6781 ggttgcgtcc cttgctgtga cgcgcgcgta tcgtgtcttg tccggcgccg cagggattca
     6841 gcctgccgat gcggctctcg cggcttgacg cagcagcgcg acggcaaact tacaagcagc
     6901 atttaaaggg aggcgcgggc gctattcggc gggcacgagc gggcgctcgg ccttggccgc
     6961 ttcggcggcg ttacgcgggt tgcggtagac gaagggcgcg ggcggccgcg aggcctcctc
     7021 gcgcgcgagc cgcaccatct cgccgtgata gggcgagagc gcgcaagccg ggtcggtgtt
     7081 gcgggcgtct cccgtgatgg ccatggcctg gcagcggcag ccgccgaaat cgatctccct
     7141 gaaggcgcaa gaccggcacg gctccttcat ccagtcggtg ccgcggaagg cattgaaagc
     7201 atcggagttg agccagatgt cgagaagcgg gcgatcctgc acattgtcga agcggagcgt
     7261 cttgatcgtc tcggcggcat ggcaggggag caccttgccg gccggggtga tattcataaa
     7321 gcccttgccc cagcctccca tgcagggttt gggacgcttg gcgtagtaat cgggcacgac
     7381 catgtcgatg gtgagcacgc ctttcagccg ctcgcgctcg cgctccacga gctcgatgga
     7441 cttcagcgtc tcgtcatagg tcgggatcag ggcggcgcga ttgagatagg cccagccgta
     7501 atactgcaca tgggcgatct cgagacgctg cgccccgagc ttgacggcaa gctcgatata
     7561 gcggccgaca ttgtggatgt tgtggcggtg aatcggagcg ttgatcgtca gcggcagccc
     7621 gagctcggtc acccatcgcg ccacgtcaag cttcttctgg gtcgcgccgg gataggcgcc
     7681 gatccgctcg gcgttctctg cgtccacgtc ctggaacgag atctggacgt ggtcgagccc
     7741 gcgcttggcg agatcctcga gacgggcgcg attgagggtg acggcggccg tgatcaggtt
     7801 gctgtagaga ccggcttccg acgccgccga gacgatctgc tccaggtcct tccgcgcggt
     7861 cggctcgccg ccggaaagat gcacctggag gatgcccatg gcgccggctt ggctcatcac
     7921 cgagcaccac atctcggtcg agagctcctt gttgacgcgc tcgagctcga tcgggttaga
     7981 gcaataggag cactgcagag ggcagcgatg ggtgagctcg gcgagcagcc cgaccggcgc
     8041 ccgggcgcac agcgcctcgg aagagctgcc gctcacgccc tcagtcggct cgtcatcgac
     8101 gacaagcgtt tcgcaatgtc cgttgcccct gaactcctcg ctcatccctt gatgtatccc
     8161 ttgtcagcca agccttgaag caggtcgagc acgtcggcgc gaatgacctc cgcgggcgcc
     8221 gaatactcct tggcgagagc cgccacgatc tcattcaccg ttctcttgcc gtcgcagagc
     8281 ttgagcacgg cgacggcggt ttggtcaggc gtcagcaccc tctcgggcgc caacaggatc
     8341 caccggtcgc gccccgcatc gtgccgaagc ttcagatagg ccggtagatg gggccgcgtc
     8401 tcgcctccga caatgagacg cgcaggggac gtggtgtcgc tcatgctttg cccgggacga
     8461 aggcgccggg cgggaccagc ccgggctcga cataagcgta gtgaagcgca tcgagctgcg
     8521 cccagagcac gttgcacttg aatctgagcg catcgagggc cgcggcctgc gcctcgggcg
     8581 tgcgcgcatt gcgcttgacg tattcgagcg cgaaattcgc gtcgtccggc gcctgggtca
     8641 ggcggcgctt gaaataagcc atcacctcgt cgctgatgaa gtcgtaattc tcaagcatgc
     8701 ccgagatgcg ctccttgtgg atcttggggg cgaacagctc ggtgagcgag gaggccaccg
     8761 cctcgaccag ggtcttctcc cgcacgaaat tgacataggc ctccaccgcg aaacgggtct
     8821 cgggcagggc gcctttcatg gagatgacgt agtcccggtc gagcccgagc ccgtcggtca
     8881 gcaccagcca gcgttcgatg ccgccttcct cctggccaaa gccgtcatgg tccatgatgc
     8941 ggtgaatcca gtcgcgccga agctgacgat cgtggcagcg gcttatcaag gcggcgtcct
     9001 tgcgcggcac cgccgactgg tagcagtagc gattgagcgc ccaagcctgc acctggccct
     9061 tgttcagctt gccgccatga agcagcttat gaaacgggtg cttgtcgtga tagcgctcgg
     9121 caccgatggc acgcagcgcc tgctccaatt cctcggggct catgatcttg gtcatgaccg
     9181 ttaccttgtc ctacccaatt ccgccaaaga acccgggtag gctcctccct ggttttcgcg
     9241 ctcttggtgc ccggcagcga aggccgcgga catcggcgca aaattccgca cgctagatat
     9301 aggaagcgtt gctacgagtc gcatgatccg cgcatgaacg ccaagtggat agcacattaa
     9361 gagaatctga acggcgagag taggcaatgc gacgatccat ggtaatcaag gtttcgggtc
     9421 gcggctacag aagatgcgcg gtagcgcata cgcggcaggg gaattagggt tgcgatcagg
     9481 gggaggctgc cacgctcaca atccaacttg tcatccttgt cgcggccgcg ctttacgcgc
     9541 gggcgggtgc cgtggcgctc aggcggccga tctccgactt ctacgtcgcc gggcggctcg
     9601 tgccgccgat attcaacggc gctgcaatcg cggtcggact cctccccgtg atcgtctttc
     9661 ccgggcttgc cggggcgctc gtccagggct cggagggggc ctcgctgctt gccatcggcg
     9721 gaggcgcggg gctcgtggcg atcggctttt tcatcgcgcc ttatctccgc aggttcggcg
     9781 gctatacggt accggatttt ctcgccgaga gattcgacct gcccgggatc aggccgcttg
     9841 cggtcctcgc cgtgatcctc tgcacgttcc cggctcttgc gctcgcgctg ttggtgcttg
     9901 gcacgctcgt cacgcgcgtt ttcgccatcg atctcgggac aggcgtcgtg cttggcgtga
     9961 cgatgctgct tgcttgcagc ttcgccgccg gcatgcgctc ggcaagtctc acgcagatcg
    10021 tccaatgcgc gatcctgctt gccgcctccc tggtcgtatt ggcgctgttg ctgtggcaag
    10081 ggggcggatt ctttcctgcg ctcggcggcg ccggcctccg ggccgcgctt gcggccatcc
    10141 agctcgaaac tttcacggct gaggatacgc tcaaccgatt cgcgttgctg ttctgtcttg
    10201 ccgccggaat tgccgcgctg ccccacctcc tcgttcgaag cttggtcacg ccatcgatcg
    10261 aaggcgcgcg gatgtcgttc ctgtgggcgc tccctttcac cgcggcactg tgcattttgg
    10321 cggcgccttc ccttgcactg ttcggcgaag tgcccgccgc ggcggacaac atgcctgcgc
    10381 tcttccggtt gggatttgcg gtcacgggcg caatcgcggc cttgcttgcg ctcggcagcg
    10441 ggcttgttct cgccctcgcc aacacgctgt catacgatct ctactacaag atggtgcatc
    10501 cgaccgcttc gaccgagcgc cgcatcttcg ttgcccgggg cgcgatcctc gcggtcgcct
    10561 tgctcgccgc ttgggcggcg ctggctcagc cggaacctac ccgcgccatg accggggcgg
    10621 tcttctcgct tgcggcaagt gcgtttctgc ccgcgctcgt tctcggcatc tggtggaaac
    10681 gcgcaagcgg cgagggcgct cttgccggca tgctgaccgg tcttgccgtg tgtctctact
    10741 acatgctggc gccgcgctac tttccgttcg cgttctacga gacgtcgagc tttctctccg
    10801 acgccacggt ggccgaggct cagcgctaca atgatttgag gcagagctac taccttgccg
    10861 atgcggcgac gcgaggagtc gccttgacgg cctgggaaga tgcggcccgc gctgtcgcca
    10921 attggggcgg cgtgaagcga gacttcgccg gcctgttcgc cgtgccgatc ggtctgttgg
    10981 tgatgatcgg cgtgagtctg ttcacgccgg caccggcgcg agagatccag agcttcgtcg
    11041 aggacttgcg taagacatcg gccgcgtagg ccccatccca agccctaatc gtccgaaggc
    11101 gtggccgcgg actgcgcggg aggcggctcg ctctgcccaa gcaggcggaa ggaatcgcgg
    11161 aagcgcgtgg cctcatcgcc ggtcgcgtgg ctcttgggac cggccgtgac caacatatag
    11221 atgcggtcgc cggacggcac gacatcgatc aggtgactga gcttgccctt cttgtcgtcc
    11281 gagatcgctt caaagcccgg atatcccgca atcgtggccg cgccccgttt gcgcaagcgc
    11341 gattcgctgc ctttggcgta ggcgttcaca agcttgacgt aggtgtcgtt gtcggggctc
    11401 gggcccttgc cgggcggata ctcgaacacc accacgctat agacggtgtc ctcgcccacg
    11461 cttacctgga agtcgtattg cagcagcggg ttttgacctt cgaccggcgc gctcgtggat
    11521 ctgggcgaac ccgggaaggt cacggcaaag ccccgttcgg gcgactggaa ctccgtccat
    11581 tgcggcaccg ccgccgtcgc ttgctgggcg ggctgatcct cggcgaatgc ggtcgcggcc
    11641 gttgcggtca agagaagcac cgccaacgct gcgctttgga tcgacattga tgcctccctc
    11701 gtcgcgacct tctcgaatcg gcatcttagc cgatccgctc ttgccgttca cccgtggcct
    11761 cctgctgtca gccttggggc gaggatttca gcgcgcgctc atacatggcc tcgacgtccg
    11821 ccttgagagc ggggcgcgtc tcggggcgga gatagagcat gtgcccgccg gcataggtct
    11881 cgaccacgat cggacttgcg ccttcgagcg gcgcgagctg gttcacgaga tagacgggcg
    11941 tggagtaggg cgtgatcaag tccgtgtatc ccgtggcgat gaacacttcg agcccgcgat
    12001 tcgccgcccg cgcctcctga atgtcatcga ggacgccggc atagccttgg cgcgtgggcc
    12061 tcgttccgaa atcccacttg ccgcgtacgt ctcgattgag cagccggtag ctcatgtcgg
    12121 tcttataacc gagctcgcct tgcgcatatt ggacgaaggc gccggtccag agcggaacgg
    12181 tcgcgtcgag gacgggatcc ggtccgcgcg gccaagagct tgccgggttg gggtcgggac
    12241 cgctgacgct cccgtcatag cggctcagca cttggccgcg tgcgcgatcg aactccttga
    12301 tgaagagcga gggcggaatg cgggcaaacg tccggcgcac ggtctcgacc ggaagtcccg
    12361 tcagctccgc caccgtaccg ctcgccctgc cggcgccttc ctccgcaccg gaagcgagcg
    12421 ccaccagata gtcggttagc gcgtaacgct cggcttcttt cacggcctct gcaagcgcct
    12481 cgcgtcccgt gacacctttg ctttcgaaat tgacggcggc gaaggagggg agaccgagcg
    12541 cccagggaag cgggtcatag tcctcgccgt tgagcagagc gaattcgagg gcaggtgaaa
    12601 cgaggatgag gccgttcggc gagatgccgc cggtcttctg caacgcccgc gtgatggtcg
    12661 cgacgcggaa tccgccataa ctttcgcccg caagaaacac cggcgagacg agtcgcccgg
    12721 catcggtgag gtaaagcctg atgaagttgg cgagcgctcg cgtatcctcc tcgacgccga
    12781 agaaatcgct ctcgtccttg ccgtcccggg cgcggctata gccggtggcg acgggatcga
    12841 cgaagacgag atcggtgaaa tcgagccagc tggagtcgtt ggcgacgagg ctgggaggcg
    12901 gcccgaggag ttcgcccttg tcgcttacgg tgacggactt gggaccgatc gcgccaaggt
    12961 ggagataggc cgatgccgct ccggggccgc cgttgaagac gaaagtgatc ggccgtttcg
    13021 tgtcttgcgg ctcgcgcacg taggcgacgt agaaaatgtc cgcgagcgtc ttgccttgcg
    13081 catcgcggag cggaagggtc gccgccttcg cgctgtaggg aacttcgaca ccggcaaggt
    13141 ggatgctgtg ttgcgtgatc gaggccggcg gaacgggggc gtcagccttg gcaggttgcg
    13201 tgcctacgct ctgatcgccg gaactctcct gtccgctcgt cgccgcatgc ggtcgcgcat
    13261 cggcaacctc gcccatcgtg ggtgcgggaa agaagagaag ggcggcgagc gttaccgggg
    13321 caagaaggcg gacgaggcgt ttcagaggtt ccgtcaatga tctgttcctt tggatttccg
    13381 ggaaataggt tgcttgggac aggtaggagc ggtgaggacg cgaaaccagg gtgagcgtgc
    13441 gggttttgcg gggtccgctc taactttatc cctcctccct cgtctttctc cctttcccct
    13501 cgcgggagag gggatgaggt atgcagggct ttctccgggg cgttgcaaac gcgccacgtt
    13561 caatggcgtt cggtgcaaag cgcgatatag tgataggcca caaagggttt gggggctaat
    13621 ctagccctcg cgcaaagctg cccggccagc aaacctggcc gagtgtcccg gcgttccccg
    13681 tgcctgcgtc tcgccgggag gaagcattgc gtcgcgcctc ttgatggcgg caaggacggc
    13741 cagcggcagc cttgtgcagc ttggaggctt ccgcgtgggg agcgggaagc ctcgaccagt
    13801 agcgttcaaa gttctccctg agtttaagtt ggtgtcgggc cgcgtgaggc tcgtcacgcg
    13861 cggggtcgac aggagccgca tcacatggcc aatccgatgc gtgagaaacg cacttggccg
    13921 ccggatcggg actccgatgg ggagaccgcg gccgcgttcg aggccgccgt cgccgtggtc
    13981 aaggagtccg tggtccgctc aggcgaggtt ctttcctcgg cgagggagga tctcagcgac
    14041 caccagcgct ggctcaaggc acaagccgcg gcggtagaat cggaccggga acggctcgcc
    14101 cgatggctgc aacggcagcg cgaacgccag gaagcgattg agcgccggga acaggcgcgg
    14161 gcgcgccgcc gggcaaggcg ccaggcggcc atcggttcgg tcagggacgc gatcgcgggc
    14221 gccgtgttcg ccgtgcgcgc cgcggtctgg ggatcgatct catccattat gggcggcctg
    14281 aacgccatcg acgcgacggc cgcaagaggg ctgcgctgga tcggcgcgag cattcgcgac
    14341 gcggcgctct atgtcgcagc agcgttccgc cgcgcggcac tatcgatggg gagagccttc
    14401 cgcggtatcg cgctcggcac cgtaggagcg ctccggcgcg cggtactgtg gtcacggaga
    14461 gcattcgggg gcgcctttca cttcggtttt tcttcggtcg cttccactct cggttcggtc
    14521 cgcgctttgc tcgcgtcggg cgtcgctcgc gccgggtcaa aactccaagt cgtcgcgcct
    14581 gcggccggcc acgccgtgac gcggggcttc ggcctgattg cggcctcgac gcataaggcg
    14641 tcggatacga tcgggcctcg cgttcgtgag aattttgttt ggctgtcggg ccgagccgaa
    14701 aatctcaggc gtgcgaccgg cgaggccgtt gctcctgctt tgtcacgcgt cgccgtcaag
    14761 acccgtgcgc ttgcgccgac gctttccgcg cggatggccg aggcgcgaac ggttgtcggg
    14821 tctcatttgc gggatgccgc gcacggcgcc aggggcctcc tggccacatc caagacctct
    14881 ccggaggcgg ccgaaaacgc agtatccttc cggcaacaga tagggagact tgatctgagc
    14941 cagatgctca tcatcgccgg ggctctcctg ctcgtctgcg gcaccttgat gctgggaggc
    15001 ggcctcctcc tccgtgccgg caagcccgcc gttgccgccg cctcgacctc ggagcctatc
    15061 gcctggctgt tcgagcacga cactcttgag ctcgacgagc gctcggtgtt cgccttcgcg
    15121 acgacgcccg agggcgtgcg tgtccgcggg tttgccatcg gcggcgtcaa catgtcggag
    15181 caaatcctcg atgcggtcgg agccgtcatc aagccggatc gcgaggtcaa ggacgtcaaa
    15241 cttgcgatgc gcgtcgtagc gcccgaagaa caagcgggcg agacgaagat cttcgaaccc
    15301 ggcgtgcctg gcacgatccc gccacaggcg cagttcgcgc tccagtttgc cttccccgga
    15361 gatggcggga tgacgcccga ccaagtgctc tcgacttacg gcggcgtcat gctgaaagtc
    15421 cgctacgagg tggcaggaac cgaaaaatcc ttcatccact acctgtcgcc gtcgttcctg
    15481 gagcggcagc tcgcggagat catggccggg tccaaaggat catgacccga ggtctcttga
    15541 cctcgtctct gtttccataa cgcaagctct agaagcaatt gagcgtgagc gtggcgcagc
    15601 ccctgatgct gagacacgcc ttgaagttgt tgcagcacct caggccgcag cccgtgaaat
    15661 cgaaggaggc atcgagcgtc tcggcaaagc aaatgctgtt gcaggaccgg aagtcgtcgt
    15721 tgcagacacg gacgctttgg ctcccgaggc cgcagggccg ctggcttggt cggaccgact
    15781 gaaagggggc ctcctgttcg gtcgtggtct gcgcccgggc accgctggcg gcgagacaca
    15841 ccacgagcgc aagagaaaga agcacaaatc tggtcatgcc gctgttctgt ccccacgcgc
    15901 ctggcgcatt cccctaagga acgtcccccc agacgcccac gcccgtgaag agcaagcttt
    15961 gcgttctccg caccaccaaa cggccaaaaa ggcgcgcgag gttggcctcg gaaaaggggc
    16021 caaacgtgcg attcactctt gccgtcctcg gtttgctcat cccgctcgcg gcggtcctgg
    16081 gctgcgccac gcaaaacgag gcgtcccggg cggccgaaca ggcggtggcc gacagcgagg
    16141 acgaggcgac atgccaggag aagagcggtg acgagcccaa ggcctacaag gcttgccgcg
    16201 aggccatgtc agacgcgcgg gcgcaacaag ccgccattca ggagcaaaag cggcgggact
    16261 tcgaccgcgt gctcggcgcc ggcaccgacg gcctgaacaa ttattgacaa atgttggggc
    16321 ttgggtggtg ggggctgggc agccaacgcc cacggccaaa accgatgcca tagttttttt
    16381 acggtgattc ggcgaaggct gccccgccgc gggagttccg ctaaaattgg tgccatcgag
    16441 gcttgttcgc aaaaggggag accgatgaac cgcaaagcac gatttctcgt cattgccggc
    16501 gttgcgattg gtacgctcgc agctgttctc aatgtttcgg ctccggtcga agccggaacg
    16561 tgtcagccgg tcaaggcgaa ggggaaaggc cgcgacatcg ctacggcatc gacccttgcg
    16621 caagccagcc tggcggtaaa agcggccgcc cttggcggca cggtcacgca gacgtcgagc
    16681 aattgcgtgc cgggaccaac ggtcgtcgtg tgcaggatat cggcggtcgt ctgcccgagg
    16741 tagcttcgag gcatcgcgaa ccgcctccgg tcccatccgc atttgtagtg ccggcaggcg
    16801 gtaaggcgtc acccgccctc gattttgctg gtcgataggg tccgcgcccg ctcgcttagg
    16861 cgggccgaca agatataagt ctttgggccg gtgaaagagg ggcgcgacct ttcgcggttg
    16921 agggtgaacg ctacgtcatc gatcctcgat ccgttcacgc tggcgggcat tgccggcacc
    16981 gcgctcatca tcgtggccta tttcgccaat cagcaaggct ggctcaaggc ggatgattgg
    17041 aagtatccgc tcgccaattt gatcggggcc gtgctgatcc tggtttcgct gattacggcg
    17101 tggaatctgc ccgtcgccgt gatcgagagc ttttgggcgg cgatcagcat ttatggattg
    17161 atcaagaacg ccggacgatc gaaaggctag atcaggtcca accagcgctc gagcatgatc
    17221 cggcagtggc gctcgaacgc gggcaaccgt gtcgcgctct cggcgatgat cgcctcggcg
    17281 tcgaaaccgt tcgccgccat gtagtcgcga ttgttgcggc acatgatttc gagcaagggg
    17341 cgatcggctt cggcgtgata ttggaagcca taggcagagt ttccgtaacg gaacagctgg
    17401 cattcggtca tgtcggaata ggcgagcctc tccgcgcccg gcaccttggc gatgatatcg
    17461 ccgtggaaat ggtaggagag aagcttgcgc ggatgacctt ccatcaaggg gtcgttcgcg
    17521 ccggcttcgg tcagcctgag atcgtaccag ccgatctcct tgcgctttcc cgggaccacc
    17581 tcgccgccga cggcgcaagc gaggagctgg gcgccgaggc aaaagcctgc aaccggcttg
    17641 ccttcgccga tgaagtcgcg gcagagctgc atctcgcccg agaggtaagg atattgatcc
    17701 gtctcgtagg cgctctgcgc gccgccgagc acgacaaggg cggagaacgc gtcgcttgga
    17761 agcctggcat agtccgcttc ccaaccgaga acgctgcgtt gctcaagacc gcggacgtca
    17821 atcaaagtcg ccaacgcgcc cgcgccttcg tgctggtcgt tccgaaggat gagaatgggc
    17881 ttcatgatcc gatccgtccg cttgaggcga gggcgtttct cgcgctgccg tcgaggatta
    17941 tactcgcctg cgtgaattga cgccttcttt gcctcaatgc atcggctgca gaaagcgaaa
    18001 tccgaccgac ggctaagccc gccgcgattg ggttccaaca caaggagggt tggtcatggc
    18061 ttaatacatc ctttcggatc ccaacttcag cgacggcacg cggcgcgagg tcatcgagca
    18121 gatcgttgat ccgtttcgcg gacgccccgg ggtgaaactt atcggctatg agccggatcc
    18181 cgacttcgat cgccttccca tagaggcgct cgggcggctt gaaccgatga aggaggcgct
    18241 gctcgccgcc gccggcaagg cttacgagct catcgacatg gagaagcagc gcggcgccca
    18301 tccgcgcatc ggcgcggtcg atacgatcga gatctatccc gccaaggaca tcaccatcga
    18361 ggaatgtcgc gatttcgccg aggagctcgg caacgagctg ttcaagcgct acgcagtgcc
    18421 ggtctatttc accggcaaga acgcgcgcag gcccgagaac gagggtctca ccttcattcg
    18481 caagggaaac tacgaggggc tcaaggaggc cgtgctcaaa gacccttcgc gcgcgcccga
    18541 tcttggcccg gccaggctgc atccgaccgc gggcgccacg atcgtcggcg ccgtcgatga
    18601 gcacgacgcc tatttcaacg tggtgctcga cacgacggac ctttcgattg cgaagaaact
    18661 cgcaagctat gtgcgcggca agaccggagg cttcaccaac attcagggcg tgatcggcct
    18721 gccgcagaca caccgccgta ccggcaaggc ttgcgtggcg gtgtcttgcg agatcagcaa
    18781 tccgctcaaa acgccgatcc atcgcgtctt caatcttctg agatctgagg ccgcccgtta
    18841 cggcgtgaac gtcatcgccg ggcagatctg tgggaccatc agcgccgagg tcctggttca
    18901 gaccgccgaa tggtacctca tgctcgaggg catcaacggg ccttgggact acaagagcca
    18961 gatcctcgaa aaccacctgc tggagcttga gagctagcga cctgcctcca ggcacaggcg
    19021 cttctctaca agaaagtcgg tccgactgcg gcgaaacctt gaagtcgcga aacctgaagt
    19081 cgcgaccgag cttcggtcca cgctgcgaaa gctgaggctt ttccgcgccg gtcggtcgag
    19141 ccggctggag atatccacag gcgcgttggc gaaaaaaatg ggggtttttg gatccaactc
    19201 gccgtttggc gcgttaggcg tgtgggcact acccggagga gatgaacatg gcgaagccaa
    19261 gccagccccg gcgcccccgc gcactgatcg tggaagacga aatcctgatt gcactggggt
    19321 tagaagcaga tctgaatgag ttgggcttcg atgcgtgcgg ccttgccgca aacgcaagac
    19381 aggccctctc ttttgcgatg gaagacatgc ccgatctcgc ggtggttgat gtttacctgg
    19441 acggcgtccg tgacggcatc gagacggcaa aaacgctccg cgatttttgc ggcgtgccga
    19501 ttgttttcgt aacggcctat ggccacgagc aggggatcat cgaacgcatt cagcgacagg
    19561 tcccaggtgc tccaatcttg agtaagccgc tctacggcag ccggctcgcc gaagcgatcg
    19621 ccgaagtggt aaagattgcc ccttcgcgcc gtgcgcagcg agcggcagcg catttgcacg
    19681 cgccggctgt ccactaggtg tcaagcgaag cgggctcgag cggcgttcgc agcttgtatc
    19741 ggctcgtctt cgcgccgggt ggctaggtgc ccggcaatcg tcgccacgat ggcccgtggt
    19801 cagccgctgt aagcgacttc gggttgacta ctgtcagttt ggaagaaaag ttataacgcg
    19861 aaagttatat ggtgtggcaa gtcgctatag tccgagagag taatgcctgg cgtaaggccc
    19921 gagccgtctg agcaagcaac ggagccaccc aatccggcgt caggccgaac gctcgacgca
    19981 cctccgctgc gttcgatgct ctccacggac gagttgctac gacgtccgtc gcgtcctccc
    20041 gatcatgcgg cggaaaatca agccctcatc gatctcatgc aggcgttggc gacctcgccg
    20101 gaagttatct tacagaggct cgccgacacc gccttgaaac tgtgccgcgc gcactccgcc
    20161 gggctcagcc tgctggagga taccgatcag aaaagcaatt ttcactggcg cgcgatcgcg
    20221 ggccagtggg cgcctcacct gaatggagga acgccgcgca acttcgggcc gtgcggcacc
    20281 gtcctcgacc gaaacgccgc gatggtatgc tcgcatcccg aactcgactt cccctacttt
    20341 gcgccggtca agccattgct cgaggaagcg ttgctcattc ctttctacgt caaaggcgag
    20401 gcagtcggta cgatttggat cgttgcgcac gacacgagtc gccgcttcga cgccgaagat
    20461 ttgcgtgtga tgaccaatct cggcgccttc gccgcagcgg cctatcaaat gtggctgtcc
    20521 ctgaacgcga ctcagcgaat tgtcgctatc gtcgagtcca gtgacgacgc aatcgtaagc
    20581 aaggacctga atggtatcat ttcaacttgg aaccaggcgg cggagcggat cttcggctat
    20641 gcggccaagg aggtgatcgg caagccgatc acgattctga ttccgccaga gcggattgac
    20701 gaggagggca aaatcctcga acggatccga agcggtggac gtgtcgaaac ttatcagacc
    20761 gtgcgcctgc gcaaggatgg aagcccggtc gatatttcgc tgaccgtatc gccgctcagg
    20821 accgccgagg gtcaagtcgt cggcgcgtcg aaaatcgccc gcgacatcac cgagcacaag
    20881 cgtagcgagc agcagattgc tattctcatg ggcgaagtcg agcaccgtgc caggaacgtg
    20941 ctggcgacgg tgcaggcgac cgtccatctc tcgcaggcag atacaccgga cggtctcaag
    21001 cgggccattg agggacgcat tcaagcgctc gcgaacgtcc acaaattgtt cgtggaatcg
    21061 cgctggtctg gagccgggct tgaggttctg gtcaaggacg agcttgccgc ctacggcgtg
    21121 gacggcgaaa ggcgcgcgca tatcgagggt ccaccggtgc tgctgcggcc ggacagggcg
    21181 caggcaaccg cgattgcatt gcatgagctc gcgaccaacg cggccaagta cggggctcta
    21241 tcggcgggcg gcggccgcgt tcacgtcgcg tggtcgctta acccggacgg aggcttcgtc
    21301 cttcgctgga acgagagcgg gggcccgctc gtcgaaccgc ccatgggcaa agggttcggc
    21361 atgcgcgtga tggagcgcat cattcgcgac caagtcaagg gcgagatacg tttcgactgg
    21421 cgccgggaag ggcttgtgtg cgagatcgcc ctgccggccg cgagatagac gtttcgtcct
    21481 tgatgcccgt cgtcactaga cggaactccc gttagcgcca tcagtttatc tcaacgcgtg
    21541 caacctgttc ggtcggcgcg ttcgggcgcc tgatcggcgt ctccacagac aggcgccgga
    21601 cagaaatgaa aactccgcca tggcagagac cgtcgccgat ttcatgcttt cgcgcctttc
    21661 gcaatggggc gtacggcgga tctacggcta tcccggtgac ggcatcaacg gcattctggg
    21721 cgcgctcgac cgcgccctgg atcgctttca attcgtgcag gttcggcacg aggaggaagc
    21781 cgccttcatg gcgaccgcgc acgccaagtt cacgaacgag gtgggcgttt gcctcgccac
    21841 ctcgggcccc ggagcaattc atctcttgaa cggcctctac gatgcgaagc tcgaccatca
    21901 gccggtgctc gccattgtcg gacaatcggt gcgcgcggcg cttggcggcg cgtttcaaca
    21961 agaagtcgac ctgcattcgc tcttcaagga tgtcgcgggc aactacgtgc aaacttgcac
    22021 cgcccccgtg cagatgcgtc atcttgtcga tcgcgccatg cgcatcgcgc acgcggagcg
    22081 ctgcgtcacc tgtctgatcg tcccgaatga cgttcaagac ctcaaagctg aagagccgcc
    22141 gcatgagcat ggaacgatcc attccgggat cgggtactcg gcgccctgca tggtgcccgc
    22201 cgaggccgag ctgcggcgcg cagcggaggt cctgaacgaa ggcgagaagg tggcgattct
    22261 cgtcggcgcc ggcgccttgc aggccaccga cgaggtcatc aagatcgccg aaatgctcgg
    22321 cgccggcgtg gccaaggcgc tgcttgggcg tgctgctttg cccgacgatc tcggcttcgt
    22381 gaccggacag atcggcttgt tgggaacgcg tccctcttgg gacatgatgc aggactgcga
    22441 cacgcttctc atggtcggtt cgcggtttcc ctatgcggag ttcttgccga aggaaggaaa
    22501 cgcaaggggc gtgcagatcg atatcgaccc caaggtcttg tcgatccgtt atcccatgga
    22561 ggtcaatctc gtcggcgacg ccgcggcaac gctcaatgcg ctcctgccga tgctggagcg
    22621 caagagagat cgatcctggc gcaacgctct cgagaccaag gtcgccgaat ggtggaaaga
    22681 ggaggaggag cgcgcgcatc tggacggcga cccgctcaat ccggaacttg tgttttggga
    22741 agcgtcgcct cgccttccgg acaaggcgat catcgccgcc gactcgggca cttcggccag
    22801 ttggttcgcg agagccctga agctccggcg cggaatgaag gcgtcgttgt ccggcactct
    22861 ggcaaccatg tgctgcgcca tcccctacgc gaccgccgcc aaattctgtt ttcccgatcg
    22921 cgtcgcgatc gccttcgtgg gcgatggcgc catgcagatg ttgggcatga acgggctcat
    22981 caccattgcc aaatattggc gggaatggga ggacccacga ctcattgtag tggtgctgaa
    23041 caatcgcgac ctcaacatgg tgacttggga attgcgcgcg atgggaggga cgccgaagat
    23101 cgaggagacg caaaacttgc ccgacttcca atacgcgcag ttggccgaga tgctggacct
    23161 caaaggcgtg cggatctcgg cggccgaaca agtcgtccct gcctgggaag cggctttggc
    23221 ggcggatcgc cccttcgtca tcgatgccgt cgtagacccg acgattttcg ccgtgcctcc
    23281 gcacgtgacg ttcgagcaga cgaaaaatta cctcgctgcg atcctgaaag gtgaccgcga
    23341 ggcggcgtgg accatgtggc gttcgttgaa gcagatggtc agctgacgct ggaagcgcga
    23401 caaggcgcgg cccactcgta gcgacttccg cctccgtgct atgcatgagg aagaagcctc
    23461 ccgtttgcag gcacaagcag actcatgtgc gagatcatca agtggacgtt cgcggcggct
    23521 tacgctgtgg ctcttttctt gctcgctgcg ggcagctggg gttggcccgg cgccgaccgc
    23581 gacccactct cagccctgct tctcatagcg ctggggctgc cgtggaaccg cttcatcgac
    23641 tatttcggcg gcacctctcc ggcggtattg gccatgctgg cgcccgccct taacctcgcc
    23701 attctctttt cgatctgctc caccctgcgc aggcgacgaa accgagcgcc cggggcgcct
    23761 tgaagcacgc aacaatccga gacatcgggt tgcatcatcc ttaatcgagg ttcctcattc
    23821 ctttggttgg gcaggagagt tccagaatat ttttgggagt cgactggacg gggcgacggc
    23881 caaggaaagg aattccaatg gacccagtac ctaattgtca tctgccattc atcggcgtga
    23941 ccgtaggctt gttgcgagct gtcgtgcgtc tcgcggtgag acgggcgaac ctgccggagt
    24001 ggcgtctcgt accgatcaac actacaccac gccgttaagt cgggtactcg cccagcggcc
    24061 tctaggtcgg ggcgaagcgg atcggaaacg ttcctgctgc gctcggcgcg cggggcgttc
    24121 cttggtgcat agagacaggg ggcgctacgg actcgcggga gccccctttt cagatgcttg
    24181 aatttggtgc cgggtgaggg atttgaaccc ccgaccttcg gtttacaaaa ccgctgcact
    24241 accactgtgc taacccggcg cctctcgatt cgtccgcgag tggctcacgc gcgaaagtct
    24301 tagacccgtt gcgcccgctt ttccacagtc ccttaccact gtccctatgg agtcgatcga
    24361 cgcgcctttg ccggcggggt gacggcttca agcctatctg cggcggctcg ccagaagtcc
    24421 ggcgatgaac gcaatggcga gcagacccac gggccccacc tgctgcatcg cttttccaac
    24481 gctctgatcg agcaggccta cgctgtcgcc cgccgatgcg aacgggctcg acgcggtcgg
    24541 cttcggcctc cgcgccgtga ggaaaaagac ggcaagcacg acgagggcga gcgcggcgag
    24601 cagcgcggca atcaggcacg cggcctgggg cggcgtgaag ccataagaca ggatcagggc
    24661 ctgataggcc gcgaacagac cgaagcaggc ggctgcgatc aggagcagcg cggcgacggc
    24721 gacgaagagc gcctggcgaa gcgagcgctc gatcgattcg cgaatgcgaa gtccgatctg
    24781 gagcgaggag atgagcgcga gcgccgagcg gagcatggag gcttcggcct agcggcgggt
    24841 gaaaacaccg aacagaaagc caaggcctac ggcgatggca acggcctgga gcggattgcg
    24901 cctgatggcc tcttcggctt cggtcgcggt cgcgatcgcc ttgtcctgca cgcggttcac
    24961 ctgcttgccg gcgatgcggc cgacggtcga ggtcaggttt tggaggtcgg cgcggatcgc
    25021 ttcgatctgg ttcgccagtt cgtcggcatc gcggtcaccg ggcccgcgtc tgcggtcgct
    25081 atcaattgat gacatggggt cgttccttgt tttgaggggg gcggaaagac agggcaactc
    25141 cttcgggcgg gccgcgttcc aagcattttc aagcggcgcg ggcgcacgcc tcgacttgat
    25201 cggccgccga ccgtcgctgt caagggccgt gacattgacc gtgacttcgg cgcaacgccc
    25261 tttgggggtc atggttgccg aagccttgcg cccgacaaag cggcctcgta gactttgagt
    25321 ccgccgaggg accgctcagg ggggatgagc atgggttgtc gcgggcttgg ccttgccact
    25381 attgccgccg ctagcctctt cgtcttttcg gcgtttgagc ccgcatacgc tgcaagcgcc
    25441 ggtctctctg ccatgaaggg tcggtcttcg ttcacggaga gcgccgcgcc gatccaggag
    25501 cgcgtgctgc tgcatcatcg tcgtcgcgtg cagtccttct actgctatcc gcggaattac
    25561 tggtggttct accggccgta caccacggcg ctcgacggcc atccccgctg catgccctac
    25621 ttccattatc ttgggcctta cgaccgccgg ggcgtggggc ctgaccgcta cgtcaaatag
    25681 gcggggcggc gcgctggctt cggtcccagc cgtccagggc gtgtactcat aaaatcatcc
    25741 ggaaaaggtc cgctttaccc ccaatagcgg acgtgcggcg agccgctcaa agagtccgtt
    25801 ttgcgccatt agcggacatc aaccatcacc tattcagagc acggtcgtcc agtgcacccg
    25861 ttgaaatccg cccggcaacg ctttggcacg gctcgctaca gtcagcagtt gcttcagcta
    25921 cttgatctcc caggtggcct tgtggttgac gatataggcg gtgcggccgc cgaccgtgtc
    25981 gtgaagttgc accacggtgt agggaaccgt tcccgtgata cctttatatt tgcccgtacc
    26041 acccatcagg tagtggtttt tgtcgtcgaa ggtcgtgaat aggttgtcac catctttgtc
    26101 ggtttcaacg cagctcccgt tgaagtgaaa tgtctctccc accgagctct ggtgatagag
    26161 gcagcgaact gacatgtcgt ggaacgggcc gtctcctttg acgttgcggt tgattccagt
    26221 gctgtcaacg attgtccccg tgccggctcc gctatcgatt ttagccacag ggttttctac
    26281 atagtacgtg tcgtattccg cgctgccggt cttcggaaac tcggcagctg ctgctg
//
LOCUS       DASUQP010000006         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1638218, whole genome shotgun sequence.
ACCESSION   DASUQP010000006 DASUQP010000000
VERSION     DASUQP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638218"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /gene="modA"
                     /locus_tag="VFY74_00310"
     CDS             complement(<1..729)
                     /gene="modA"
                     /locus_tag="VFY74_00310"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5930185.1"
                     /translation="MLVLALVLAAAPLFTVPARAADITVFAAASLKNALDDAVKRYET
                     KTGVKVVTSYAASSALARQIEAGAPADIFFSADFDWMDELQKKNLIDVPSRHTLLGNT
                     LVLIAPKDSTVSLAIEKNFSLLQALGPDGKLAMASVDSVPAGKYGKAALTYLGVWDAV
                     APRVAQADNVRAALAFVARGEAPLGIVYGTDANAERAVRVVGTFPAESHPDILYPVAL
                     VAGAKSGARQFLEFLFSPEAAPAFE"
     gene            912..1607
                     /gene="modB"
                     /locus_tag="VFY74_00315"
     CDS             912..1607
                     /gene="modB"
                     /locus_tag="VFY74_00315"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX5930186.1"
                     /translation="MFTLTPEELVAIKLSLRVAIVATAASLPIGILIALALARGRFLG
                     RGLLNALVLVPLVLPPVVTGYLLLVTFGRNAPIGHWLAEHLGIVFAFRWTGAALASAI
                     MAFPLLVRAIRLSIEAVDRRLEQAAATLGANRLSVFATVTLPLIAPGIFAGIILAFAR
                     AMGEFGATLTFVSNIPGETQTLPMLIHTFTQVPGGDAAAFRLTVVSLAISLGAILASE
                     ALAQRVSRRLHAV"
     gene            1604..2290
                     /gene="modC"
                     /locus_tag="VFY74_00320"
     CDS             1604..2290
                     /gene="modC"
                     /locus_tag="VFY74_00320"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEX5930187.1"
                     /translation="MSLEVSAEARLGDFALDASFKSDSRVTAIYGPSGSGKTSILNVI
                     AGLLRPRRGRVAVDGAVLLDTATGAFLPPHRRRIGYVFQESRLFPHFSVKGNLRYGRM
                     FATSSRHHASFEEVVDLLALGPLLRRGTSALSGGERQRVAIGRALLSSPRLLLLDEPL
                     SSLDDRRKAEVMPFLERLRDQARMPIVYVSHVFSEIERLSGTVVLMGNGQVLDVDRTS
                     HVKAKTIEPR"
     gene            complement(2410..>2684)
                     /locus_tag="VFY74_00325"
     CDS             complement(2410..>2684)
                     /locus_tag="VFY74_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930188.1"
                     /translation="CPHSQVKQGDTCVDVTPPAAEPAPEPAPKKVPQEEKAAGDQEAT
                     RLSARILPQPESASLRTSQGRAATAAAMPVWHDPDRTALRAHPDPP"
BASE COUNT          413 a          891 c          860 g          520 t
ORIGIN      
        1 ctcgaaggcg ggggcggcct cgggcgaaaa cagaaactcg agaaattggc gcgcaccgga
       61 tttcgccccg gccaccagag ctaccgggta gaggatgtcc ggatggctct ctgcggggaa
      121 cgtgccgacg acgcgaaccg cgcgctccgc gttcgcgtcg gttccgtaaa ctattccgag
      181 cggcgcttcg cctcttgcaa cgaacgcgag ggccgcgcgc acattgtcgg cctgcgccac
      241 acgcggcgcc accgcgtccc acacgccgag ataagtcaac gccgccttgc cgtatttgcc
      301 cgcaggcacg gagtcgacgc ttgccatggc gagcttgccg tcggggccaa gcgcctgaag
      361 cagcgaaaaa ttcttttcga tcgcgagcga aacggtcgag tccttcggcg cgatcagaac
      421 gagcgtgttg ccgagaagcg tgtgccgtga cggaacgtcg atcagattct tcttctgcag
      481 ctcgtccatc cagtcgaaat cggccgaaaa gaaaatgtcg gccggcgccc ccgcctcgat
      541 ctgcctggcg agcgccgagg aggcggcgta gctggtcacg actttaacgc cggtcttggt
      601 ctcataacgc ttgacggcgt catcgagcgc gttcttcagg cttgcggcgg caaacacggt
      661 gatgtccgcc gcgcgcgcgg gtaccgtaaa caggggcgcg gcggcgagga cgagcgcgag
      721 aacgagcatg ccgcgccggc atgtcataag gcgcatcggc gaactccttt gctatgggtg
      781 gggggatccg atatggtcac atggaaatat cgcctgaaat cgccatagcg taaaccgtcg
      841 cattcgtcgt agcgtgtaag gcgctaggtc cggtcggcca tggccgcgcg actgatgatc
      901 gaggatcccc cgtgttcacg ctgacgcccg aggaactggt tgcgatcaag ctctccttgc
      961 gcgtcgctat cgtggcgacg gcggcgagcc tcccgatcgg catcctgatc gcgctcgcgc
     1021 tcgcccgggg ccgattcctg ggccgcggtc tcttgaacgc gctggtcctt gtcccgctcg
     1081 tgcttccgcc cgtcgtcacc ggctacctgc tgctcgtgac gttcgggcgg aacgcgccca
     1141 tcgggcactg gctcgccgaa catctcggca ttgtcttcgc cttccgctgg acgggcgcgg
     1201 ccttggcctc cgccatcatg gcctttccct tgctcgtgcg cgcgatacgg ctgtccatcg
     1261 aggcggtcga cagaaggctc gagcaagcgg cggccacgct cggtgccaac cgcttgtccg
     1321 tcttcgccac cgtgacactg ccgctgatcg ctcccggcat ttttgcgggc atcattcttg
     1381 ccttcgcgag agccatgggc gagttcggcg ccactctcac cttcgtgtcc aatatccccg
     1441 gagaaacgca gacgctgccg atgctgattc acactttcac gcaggtgccg gggggcgatg
     1501 cggccgcgtt ccggttgacc gttgtttcgc tggcgatctc gcttggcgcg atcctcgctt
     1561 ccgaggccct tgcccagcgc gtcagccgca ggcttcacgc ggtatgagcc tcgaagtgtc
     1621 ggcagaggcg cgtctcggcg acttcgctct cgacgcgagc ttcaagagcg acagccgggt
     1681 gacggccatt tacggcccgt cgggctcggg caagaccagc attctcaatg tgatcgcggg
     1741 attgcttcgt ccgcggcgtg gccgcgtcgc cgtcgatggc gccgtcctct tggatacggc
     1801 gacgggcgct ttccttcctc cccatcgccg ccgcatcggc tacgtctttc aggagagccg
     1861 gctctttccc catttttcgg tgaagggcaa cctgcgctac ggacggatgt tcgccacttc
     1921 ctcgcgccac catgcgtcct tcgaagaggt tgtcgacctc ctcgccctcg gccccttgtt
     1981 gcggcgggga acatcggccc tttccggcgg ggaaaggcag cgggtcgcga tcggccgcgc
     2041 tctcctttcg tctccgcgcc tgctcctcct cgacgagccg ctgtcgtcgc tcgatgacag
     2101 gcggaaagcg gaggtgatgc cgttcctcga gcggctccgc gaccaggccc gcatgccgat
     2161 cgtctatgtg agccacgtgt tttcggagat cgaaaggctg tccgggaccg tggtgctgat
     2221 ggggaacgga caggtgcttg acgtcgatcg caccagccac gtgaaagcga aaacgattga
     2281 accgcgctaa ggttggcctg cgtcagatcg acgtttcgtt gacaggcgct ctccgagcca
     2341 cgccctgcga gcgccaacgc cgcgccacct tagtcgtgat ggccgccgcc tccgccaccc
     2401 ccaccgccgc tatgggggat ctggatgcgc acgcaaagcc gtccgatccg gatcgtgcca
     2461 tacgggcatg gcggcagcgg ttgcggcgcg accctgactg gtacgcagcg acgcgctctc
     2521 cggttgtggt aatatccggg cggacagcct ggttgcctct tgatcaccgg cggctttttc
     2581 ttcttggggc accttcttcg gcgccggctc aggcgccggc tctgccgcag ggggcgtaac
     2641 gtcgacacag gtatcgccct gcttgacctg gctgtgcggg cata
//
LOCUS       DASUQP010000007        13353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_411982,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000007 DASUQP010000000
VERSION     DASUQP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13353
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411982"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFY74_00330"
     CDS             complement(<1..613)
                     /locus_tag="VFY74_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX5930189.1"
                     /translation="MAAALLALPTAARADCDVAAIPDDAAFKFDPGGVRQALARGGIG
                     VGGYYYGETFYNWGGFDEGGEYDGVLELYINADMKKLGLWRGLCFFANGYQIHGNSIT
                     AANIGSLMPVSNLEATDATRLFELWFEQHLFDDKLTVKIGQLAADAEFLISEGGGYFL
                     NGTWGWPSITAADLPSGGPAYPLATPGVRVAINPNERLGLMIGV"
     gene            complement(796..2115)
                     /locus_tag="VFY74_00335"
     CDS             complement(796..2115)
                     /locus_tag="VFY74_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEX5930190.1"
                     /translation="MFRRQSINVPFAALAISAFLAAPLAAEPAPKDVLKTYADIAEAA
                     YGDSLDTARTLKLSVDALLAKPTEDNLRAARAAWIAARIPYMQTEAYRFGNAIVDDWE
                     GKVNAWPLDEGLIDYVASSYGTDSPENELYAANVIANATLTIGGKKLDVSKITAEVLA
                     DGLQEAGGVEANVATGYHAVEFLLWGQDLNGTGPGNGARPSSDFDSAKCTNGNCVRRA
                     EYLRTVTDLLVDDLAWMASQWGPGGKARKTIEDGGNEAGLTAIMTGLGSLSYGELAGE
                     RMKLGLMIHDPEEEHDCFSDNTHASHFFDALGIRNVYLGTYRRIDRSLVSGPSVSELV
                     KAKAPEIDAEVRAKLDATMEAMNALYLRALTTESYDQMVAEGNEAGNAAVQRVVDALL
                     DQTKAIERAVAALDLKAIAFEGSDSLDDPAKVETEAKAAGGESATPN"
     gene            2369..3289
                     /locus_tag="VFY74_00340"
     CDS             2369..3289
                     /locus_tag="VFY74_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5930191.1"
                     /translation="MHLREIDANLLVTLDALLVDASVTRAAERLGRSASAISHALAKL
                     REIFADELFVRAGQRLVPTAKALELAPTVHVILAGMESLLRPSKPFDPSETSRDFAVA
                     TSEAGELMLVQPLRRRLQKLAPSVRVGWTPSRLEDNTDAMRNGRAHFVIDIENIPSPA
                     PDIRVLKLCDDTLATLARPGHSLARGTPKPAAFAASGHIAVHGLPTIATIDGALAAHR
                     LACVVTSRVSSLLVGLLLALNSDALLTVPASLAALLEQQFGLERVRQPFQAIPFPLRL
                     MWHSSYDRDECHAWVRAEFARLAGEMDAGG"
     gene            3479..8698
                     /locus_tag="VFY74_00345"
     CDS             3479..8698
                     /locus_tag="VFY74_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEX5930192.1"
                     /translation="MPAWFRAGLFAALFVFATTPSLASPKTFQDDALDDAAITLEADL
                     KEEAGTVDKPLATLKQEAEAALERVDFGSAANIYVQIVTLAPDDAKAWRRLADIWLLI
                     PASGEDDGATRYERARTAAYIAYRRSATPEEEATSLVTLANAFTKREEWRQARNALKL
                     ALDLHGSPELQTVYDGLREKYGFRVANFSVDSDAASPRACFQFTESLPKRTDYSPYVA
                     VAGQDRPALSVDDQQVCVEGLKHGESYAVTLRAGLPSTVGENLLKTADFNIYVRDRSP
                     SVRLAGKAYVLPRSGQQGIPLITVNTDLVKATIYRIGDRSLIDNVLGYDFERNLQGYA
                     LDEIADQKGEKVWTGELKVEKALNQEITTAFPIMEAVPELEPGVYLLTARPGNVPGDE
                     YGERTTQWFIVSDLGLTAYSGTDGIHAYVNSLATTAPLADVELRLLARNNEVLAVEKT
                     DAGGDVTFAPGLARGEGGLAPALIVASAGDGDYAFLSLKQSAFDLTDRGVAGRDAPKG
                     LDALVFTERGVYRTGETVYVTALLRDAASVAVPGVDLTLVVERPDGVEYRRTVLADQG
                     IGGRSLAVPIIAAAPTGTWRVAVFSDPKGTSIGGAMFLVEDYVPDRLEFDLATEATTI
                     SLEAPAEVTLDGRFLYGAPAAGLGIEGEVNIAKAAERPGYPGYVFGLDPSAGDDEQDE
                     AGTESLPLADLPKTDADGKATFTVALDKLPASTRPLEANVVVRLAEPGGRAIERKLTL
                     PVVPAAPMIGVKPLFAGKSLSDNDTAAFDVVMVAPDGKPMAATGLKWQLLGIESKYQW
                     YRSDGYWRYEPIKVTRRVADGTLDIAADKPGKISVPVTSGRYRLEVSSPDTQGPATSF
                     GFDSGWYAESSADTPDLLEIALDKPEYRPGETMTVAVTARSAGKVTLSVIGDRLITQT
                     TTDVEPGLIKLPLTVGNDWGTGAYVLATLRRPLDEVEKRMPGRAIGVQWFSVDRQAHT
                     IGVALDLPEKIRPETTLRIPVKLAGLTSGEEARIVVSAVDVGILNLTNYKVPAPDAYY
                     LGQRKLSAEIRDLYGQLIDGMQGTKGAIRTGGDSGAGALQGSPPTQPPLAFYSGIVTV
                     GADGTAEVSFDIPAFSGTVRVMAVAWSADKTGHASGDVVVRDPVVVTATLPRFLLTGD
                     KSTFRLDLDNVEGASGTYEIEVTADGPLSLERADKSLTLDAKKRGAVTMPVSAIGVGP
                     GTVSVAVDGPDGFALSRSYPLTVKPATQILARRTVKPLEPGQSITIASDVFADLVPGT
                     GKVALSVTPMAALDVATLIAALDRYPLGCTEQIVSRALPLLYVSELSAKANIAADTGI
                     DGRIAKAIEIVLARQGSEGAFGLWSPGGDDAWLDAYVTDFLTRARARGFSVSDDQFKL
                     ALGRLRNYVSTAPDVATDGGLPLSYALYVLARNGMAPVGDLRYIADVKLNDLLTPAAK
                     AEIGAALAMLGDRVRAEKAFDAALGALPADPKFEGGRTDYGSPLRDAATLVTLAAEGG
                     APKVILVSGTERIETARDRVTTTSTQENAWLVLAARALGKQSVTLTVNDGAEDGPLYR
                     SFTEHEIAAGPLVVTNSGDTPLDAVVSVSGAPVTPEPAAEHGFTLARSFHTLDGAEAD
                     LGTAKQNDRFVVVLTVTEPQPEFARVALTDYVPAGFEIDNPRLVSSGDAGTLDWIANA
                     GTPVHTEFRADRFTAAFERRKGDPTTYSVAYIVRAVSPGSYVLPQAVVEDMYRPDRFG
                     RTDTGAVEVAPAR"
     gene            8695..10788
                     /gene="pbpC"
                     /locus_tag="VFY74_00350"
     CDS             8695..10788
                     /gene="pbpC"
                     /locus_tag="VFY74_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX5930193.1"
                     /translation="MSSSAKTSRWKRRALFAAVGTIWLIAAAAGALSGLVETYGPPPL
                     GRDLDASRVVLDRNGMLLRAYLTGEGRWRLAASRNDVDPRYLEALLAYEDKRYFVHDG
                     LDPLAIGRAFSQLLTHGRIVSGGSTLTMQVARLLEPRRERSLDAKIRQALRAVQLERA
                     LSKDEILGLYLTLAPYGGNLEGVRAASLAYFGREPRRLTLGQAALVVALPQAPEARRP
                     DRFPEAAKRARDRVLDRIAGAGVFSDAEIARAKQEKVPSGREPMPLLAPHAADQALKD
                     TPQERALRLTIDGRLQKALEHLARDRARALGPDMSVAIVVVDNATGEVLARVASPDYF
                     DERRAGQVDLTQAVRSPGSTLKPFIYGFGFEDGLIHPETLIEDRPVRYAGYSPENFDL
                     TFQGTVTVRRALQLSLNVPAVAVLEAIGASRLTARLQEAGAFLVLPRREAPGLALGLG
                     GIGIRLTDLTMLYGGLARQGTVAPLLERLGAAPLAPRRLIEPAAAWSVANVLAGTPPP
                     ESAAGGRIAFKTGTSYGYRDAWAIGFDGKRTIGVWAGRPDGAPVAGLMGRTAAAPILF
                     DAFARVGGRLNPLPPAPNGALIASTAKLPPPLQRFASAASTGEAMAPKVRIVFPPDGA
                     SLELSGREGEPRDPIAIKIAGGTPPLSVLLNGMPLAGKKSARTLFFAPDGPGFVRLTV
                     TDAKGTADSVVVRLQ"
     gene            10870..12156
                     /locus_tag="VFY74_00355"
     CDS             10870..12156
                     /locus_tag="VFY74_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX5930194.1"
                     /translation="MSRSAASRDGKFRGGPLIPVLFFATPLLTAVAPRLAPFLLLILA
                     LVLIVAALRRGLAWRALLEPNPAMMALAVLAAYAVLSAIWAAEPDEAVSKGLLLLAVT
                     FVVFAVAAAIPSLDAEQIRRAGIAFVAGALCAALFVTIEVLTTGALTRAAMNVIPALQ
                     PDSAKRIKIVDGRVTYIRGAVFNQHVVMLAFQLWPGLLALSTLQGPRRGLLSALFFIA
                     LALPISLSEHDSSQIGLIASALVLLLAWVWSRAAIRGLAVVWCLGFALVLPLAFLSYK
                     AELHLTKWVPQSARARIIIWEYTAERVFERPWLGIGADSTPGSKAAPAKLDKPKGFVQ
                     PRTTGHHAHNLFLQTWYELGLVGALLAAIAGAAVVLRIFLLPEPAQAYGAAAFTMFAA
                     IAAFAFGMWQVWLISAVGLLVVYLLMATAFTARGRG"
     gene            12294..>13353
                     /gene="miaB"
                     /locus_tag="VFY74_00360"
     CDS             12294..>13353
                     /gene="miaB"
                     /locus_tag="VFY74_00360"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5930195.1"
                     /translation="MNEYDSERMAEALGAEGYQPTEDPAEADLVILNTCHIREKAAEK
                     VYSELGRVRLAKERRKREGRDTLIAVAGCVAQAEGQEIMARAPAVDLVVGPQSYHRLA
                     SLLAEAATSGKGVVATDFPAEDKFAQLPPRSRQASHPSVFITVQEGCDKFCTFCVVPY
                     TRGAEYSRAPSEIVAEAERLAAQGAREITLLGQNVNAYHGQAADGRPWSLARLLRRLA
                     ELPRVARLRYTTSHPLDMDDELIALFAEEPKLMPYLHLPFQSGSDRILKAMNRKHTIA
                     DYEAVIGKVRAARPDIALSTDIIVGFPGETDRDFEETMALAARARFAQAFTFKYSARP
                     GTPAASFSGQVPEAVKRER"
BASE COUNT         2160 a         4563 c         4328 g         2302 t
ORIGIN      
        1 agacgccgat catcaggccg agccgctcgt tcggattgat ggcgacgcgg acgccgggcg
       61 tggccaaggg ataagccgga ccgccgctcg gcaggtcggc ggcggtgatc gaaggccagc
      121 cccaggtgcc gttcaagaaa taaccgccgc cctcgctgat gaggaactcg gcatcggcgg
      181 cgagctgtcc gatcttcacg gtgagcttgt cgtcgaagag atgctgctcg aaccagagct
      241 cgaacaggcg cgtggcgtcg gtcgcctcca gattcgacac cggcatgagc gagccgatat
      301 tggctgccgt gatcgagttg ccgtggatct gatagccgtt ggcgaagaaa caaagcccgc
      361 gccacaggcc gagcttcttc atgtcggcgt tgatgtagag ctcgagcacg ccgtcatatt
      421 cgccgccctc gtcgaagccg ccccaattgt agaacgtctc gccgtagtag tagccgccga
      481 cgccgatgcc gccgcgtgcg agcgcttggc gcactccgcc gggatcgaac ttgaaggctg
      541 cgtcatcggg gatggcggca acgtcgcaat cggcgcgcgc cgcagtggga agcgcaagaa
      601 gagccgccgc catcgccagc gcaccgacag cttggcgcag tttgggacga attcctccgc
      661 gactcttgtt ttggtccgct tccgatgttg cgtgcgcgcc gcgaaagctc ggccttcccc
      721 tcattgcctt tcccccaaaa cccgagtcac ccttgttacg gtgttagcca cgctagtggg
      781 gcctctccca tcgcgtcaat tcggcgtcgc actttcccca ccggccgctt tggcctccgt
      841 ctcaaccttc gccggatcgt cgagactgtc tgagccctcg aacgcgatcg ccttgaggtc
      901 gagcgccgcc accgcgcgct cgattgcctt ggtctgatca agaagcgcgt cgacgaccct
      961 ctgcactgct gcgttccccg cttcgttccc ttccgcgacc atctggtcgt aggactcggt
     1021 cgtgagcgcg cgcagataaa gcgcgttcat cgcctccatg gtcgcgtcga gcttggccct
     1081 cacctcggcg tcgatctccg gcgccttggc cttgaccagc tctgacacgc tcgggcccga
     1141 cacgagagat ctgtcgatgc ggcgataagt tccgagatag acattgcgga taccgagcgc
     1201 gtcgaaaaag tgggaggcgt gggtattgtc ggagaagcaa tcgtgctcct cctcgggatc
     1261 gtgaatcatc agtccgagct tcatgcgctc tccggcgagt tcgccgtagg agaggctgcc
     1321 gagcccggtc atgatcgcgg tgagccccgc ttcgttgccg ccgtcttcga tcgtttttcg
     1381 cgccttccca cccggccccc attgcgacgc catccaggcg agatcatcga cgagaaggtc
     1441 ggtcacggtc cggagatatt cggcacgccg cacgcaattg ccgttcgtgc acttcgcgct
     1501 gtcgaagtcg ctcgaaggtc gcgcgccatt gccgggtccc gtgccgttca ggtcctggcc
     1561 ccagagaaga aactcgacgg cgtggtagcc ggtggcgacg ttcgcctcca cgccgcccgc
     1621 ctcctgcagc ccgtcggcca gcacctccgc ggtgatcttg ctgacgtcga gcttctttcc
     1681 gccaatcgtc agcgtggcat tggcgatcac attggcggcg taaagctcgt tctcgggaga
     1741 gtcggtcccg taggacgaag cgacatagtc gatcagcccc tcgtcgagcg gccaggcatt
     1801 caccttgcct tcccaatcgt cgacgatggc gttgccgaag cgataggcct cggtctgcat
     1861 gtaagggatg cgcgcggcga tccaggcggc gcgcgcggcg cggagattgt cctcggtcgg
     1921 tttcgcgagc agggcgtcga cgctgagctt cagcgtcctc gcggtatcga gggaatcgcc
     1981 ataggcggcc tctgcaatgt ccgcataagt tttcaggaca tccttcggcg cgggttcggc
     2041 cgcgagaggc gcggcaagga acgcgctgat cgccagagcg gcgaacggca cgtttatcga
     2101 ttgtcgtcgg aacatcggtg ccctttgtcc tcacgatacg aaaacacgcc cgccggcttt
     2161 cgtccggcga gacgcgttcg acggagataa atgcggcagc cttaagggcc gggaccctca
     2221 ggccgccgca aggttgagat gagtaaacgg ccagctttcg cttggacgtt aaggcctccg
     2281 ggctgggcag ccggatgcct tgtcacgacc ctagcgccag agctaacatg cacaaagtga
     2341 actcttttca ggagaattat tgcaccccat gcacctaagg gaaatcgacg ccaatcttct
     2401 cgtgacgctc gacgccctcc tcgtcgatgc gagcgtcacc cgcgccgcgg agcggctcgg
     2461 aagaagcgcg tcagcgatca gccatgcctt ggcgaagctc cgcgagatct tcgccgacga
     2521 gctgtttgtg cgcgcgggcc agcggctcgt gcccacggca aaggcgctcg agcttgcgcc
     2581 cactgtccac gtcatcctcg ccggcatgga gagcctgctc cggccgagca agccgttcga
     2641 cccctccgag acctcgcgcg acttcgcggt cgcaaccagc gaggcggggg agctgatgct
     2701 ggtgcagccc ttgcgacggc ggctgcaaaa gctggcgccg agcgtgcggg tcggttggac
     2761 cccctcgcgc ctcgaggaca acaccgatgc gatgcgcaac gggcgggccc atttcgtcat
     2821 cgacatcgag aatatcccct cgccggcgcc cgacatccgc gtcctcaagc tctgcgacga
     2881 cacgctcgcg acgctcgccc gcccaggaca ttcgctcgca cgtgggacgc ccaagcctgc
     2941 ggccttcgcc gcctcgggcc acatcgccgt gcatggcctt cccacgatcg cgacgatcga
     3001 tggggcgctc gcggcgcaca gactcgcctg cgttgtcacc tcgcgcgtgt cttcgctcct
     3061 ggtcggcttg ctgctcgcgc tgaacagcga cgcgctgctc acggtcccgg catcgcttgc
     3121 cgcgttgctc gagcagcaat tcgggctcga acgcgttcgc cagccgtttc aggcaatccc
     3181 cttcccctta cggctcatgt ggcattccag ctacgaccgc gacgagtgcc atgcctgggt
     3241 ccgggcggaa ttcgcgaggc ttgccggcga gatggacgcg ggtggctgag aaaccgccag
     3301 cgggccatgc cgccgaggcc ttgatctccc gggccgccgc gcttttcggc tgtcctgcgc
     3361 ttggcgagct cgacgattaa gatccaaaac gcgacctccc tctggtttct gtcgcaaggc
     3421 tcgtttacga taggggtctg cacccgtatt gccgagtacg ggtcccggag gtccctcgat
     3481 gcccgcatgg ttccgcgccg gcctgttcgc cgcgctgttc gttttcgcca ccaccccttc
     3541 cctcgcctcc ccgaagacat ttcaagacga cgctctcgac gacgccgcga tcacgctcga
     3601 ggccgacctc aaggaggagg ccggcaccgt cgacaagccg ctcgccacgc tgaagcagga
     3661 ggcagaagcg gcgcttgagc gcgtcgactt cggaagcgcg gccaatatct atgtgcagat
     3721 cgtcacgctc gcgcccgacg acgccaaggc ttggcgtcgg ctcgccgata tctggctcct
     3781 gatcccggca agcggcgagg acgacggcgc gacacgctac gagcgcgccc gcacggccgc
     3841 gtacattgcc tatcggcgat ccgcgacgcc cgaggaggaa gcaacctcgc tcgtcacgct
     3901 cgccaacgcc ttcaccaagc gggaagagtg gcggcaggcg cggaacgcgc tgaagcttgc
     3961 gctcgacctg cacgggtcgc ccgagcttca gaccgtctat gacggcttgc gcgagaaata
     4021 tggcttccgc gtcgcaaatt tcagcgtcga ttccgatgcg gcgagcccgc gcgcctgctt
     4081 ccagttcacc gagagcctgc cgaagcgtac cgattattcg ccttacgtcg cagtcgccgg
     4141 gcaggacagg cctgcgctct cggtcgacga ccagcaagtc tgcgtcgagg gactgaagca
     4201 cggcgagagc tacgccgtca cgctccgcgc aggacttccc tcgaccgtgg gcgagaacct
     4261 gctcaagact gcggatttca atatttatgt gcgcgaccgg agcccctcgg tcaggctcgc
     4321 ggggaaggcc tatgtgctgc cgcgcagcgg ccagcagggc atcccgctca tcaccgtcaa
     4381 taccgacctc gtcaaggcga ccatctaccg gatcggcgac cggagcctca tcgacaacgt
     4441 gctcggttac gatttcgagc gcaatctcca gggctacgcg ctcgacgaga tcgccgacca
     4501 gaagggcgag aaggtctgga ccggcgagct caaggtcgag aaggcgctca accaggaaat
     4561 caccaccgct ttcccgatca tggaggcggt cccggagctt gagcccggcg tctatctctt
     4621 gacggcgcgg ccgggcaacg tgcccggcga tgagtacggc gagcgcacga cgcagtggtt
     4681 catcgtctcg gaccttggac tgacggccta ctccggcacc gacggcatcc acgcttatgt
     4741 caattcactc gcgaccacgg cgccgcttgc cgatgtcgag ctcaggctgc tcgcccgcaa
     4801 taacgaagtg cttgcggtcg aaaagacgga cgccggcggt gacgtcacct tcgcgcccgg
     4861 tctcgcgcgc ggcgaaggcg ggctggcgcc ggccctgatc gtggcatcgg cgggcgatgg
     4921 cgattatgcc ttcctcagcc tgaagcaatc ggccttcgat ctgaccgatc gcggcgtggc
     4981 gggacgcgac gcgccgaaag ggctcgatgc gttggtgttc accgagcgcg gcgtctatcg
     5041 caccggcgag accgtatacg taaccgcgct cctccgcgac gccgccagcg ttgccgtgcc
     5101 gggcgtcgac ctgaccctgg tggtcgagcg ccccgacggc gtcgagtatc gccgcaccgt
     5161 cttggctgac caggggatcg gcggccgctc gctcgcagtc ccgatcatcg ccgcggcgcc
     5221 gaccggaaca tggcgcgtcg cagtcttctc cgatcccaag gggacttcga tcggaggcgc
     5281 gatgttcctg gtcgaagatt atgtgccgga ccgtctcgaa ttcgatcttg cgacggaagc
     5341 caccacgatc tcgcttgaag cgccggccga agtgacgctc gacggccgct tcctctatgg
     5401 cgcgccggcg gccgggcttg gcatcgaagg cgaggtcaat atcgccaagg ctgccgagcg
     5461 gccgggctac cccggctatg tcttcggtct cgatccttcc gcgggcgacg acgagcaaga
     5521 cgaagccggc accgagagcc tccctctcgc cgatctgcca aagaccgatg ccgacggcaa
     5581 ggcgacgttc acggttgccc tcgacaagct gccggcatcg acccggccgc tcgaagccaa
     5641 tgtggtggtg cggctcgcgg aacccggcgg acgggcgata gaacggaagc tcacgctccc
     5701 cgtcgttccc gccgcgccga tgatcggggt gaagccgctc ttcgccggca agtcgctcag
     5761 cgacaacgac acagccgctt tcgatgttgt gatggtcgcg cccgacggca agccgatggc
     5821 cgcgaccggg ctcaaatggc aactgctcgg catcgagtcg aaatatcagt ggtatcgcag
     5881 cgacggctat tggcgatatg agccgatcaa ggtgacgcgc cgcgtcgccg acggcaccct
     5941 cgacatagcg gccgataagc ccggcaagat ttccgttccc gtgacgtcgg gacgataccg
     6001 gctcgaagtg tcgagccccg atacgcaagg acccgcgacc tcgttcggtt tcgattccgg
     6061 atggtacgcc gaatcctcag ccgacacgcc cgatctcctg gagatcgcgc tcgacaagcc
     6121 ggaataccgg ccgggcgaga ccatgacggt cgcggtcacg gcgcgaagcg cgggcaaggt
     6181 gacgctctcg gtgatcggcg accggctcat cacccagacc accaccgacg tcgagcccgg
     6241 tctcatcaag cttcccctca ccgtcggcaa tgattggggc accggcgcct atgtgctcgc
     6301 cacgctcagg cgcccgctcg atgaggtcga gaagcgcatg cccggccgcg ccatcggcgt
     6361 gcaatggttc agcgtcgaca ggcaagcgca cacgatcggg gtcgcgctcg atctcccgga
     6421 gaagatcaga ccggaaacga cgctgcgcat tccggtcaag ctcgcggggc taacctcggg
     6481 cgaggaggcg cgcatcgtcg tgtcggccgt cgatgtcggc attctcaatc tgaccaacta
     6541 taaagtgccg gcgcccgatg cgtactatct tgggcagcgg aaactttcgg ccgagatccg
     6601 cgacctttac ggccagctga tcgacggcat gcaggggacg aagggcgcga tccgcaccgg
     6661 cggcgattcc ggcgcgggcg cgttgcaggg aagcccgccc acgcaaccgc cgctcgcgtt
     6721 ctattccggc atcgtcacag tcggcgcgga cggcaccgcc gaggtcagct tcgatatccc
     6781 cgccttctcc ggaacggtgc gcgtcatggc ggtcgcgtgg agcgcggaca agacgggtca
     6841 tgcctcgggc gatgtcgtgg tgcgcgatcc ggtcgtggtc acggctacgc tgccgcgctt
     6901 tctcctcacc ggcgataaga gcacgttccg gctcgacctc gacaatgtcg agggggcgag
     6961 cggcacctac gagatcgagg tcacggccga cgggcctctg agcctcgagc gcgcggacaa
     7021 gagcctaacg ctcgacgcca agaaacgcgg cgcggtcaca atgccggtct cggcgatcgg
     7081 cgtcggaccc ggcaccgtct cggttgccgt cgacggaccc gacggctttg cgctctcgcg
     7141 cagctatccg ctgacggtga agccggcgac gcagatcctg gcgcgccgca cggtgaagcc
     7201 gctcgagccc ggccagagca tcaccattgc aagcgacgtc ttcgccgacc tcgtgccggg
     7261 gacggggaag gtcgcgctct cggtcacgcc gatggccgcg ctcgacgtgg cgacgctgat
     7321 cgcggcactc gaccgctatc cgctcggctg caccgagcag atcgtgagcc gggcgctgcc
     7381 gctgctctat gtcagcgagt tgtcggcgaa ggctaacatc gccgccgata ccggcatcga
     7441 tgggcgcatc gccaaggcga tcgagatcgt ccttgcccgc cagggctcgg agggcgcttt
     7501 cgggctttgg tcgcccggag gcgacgacgc ctggctcgac gcctatgtga cggatttcct
     7561 cacccgggcg cgggcgcgcg ggttctccgt ctccgacgat cagttcaagc tcgcgctcgg
     7621 tcgcttgcgc aactatgtga gcacggcacc cgatgtcgcg accgatggcg gcttgccgct
     7681 ctcctacgcg ctctatgtgc tcgcccgcaa cggcatggcg ccggtgggcg acctccgcta
     7741 catcgccgac gtcaagctga acgacctgtt gacgcccgcg gcaaaggcgg agatcggcgc
     7801 ggcactcgcc atgcttggcg accgcgtgag ggctgagaag gcgttcgatg ccgcgctcgg
     7861 cgcgctgccc gccgacccga agttcgaggg cggccgcacc gattacggct cgcccttgcg
     7921 cgacgccgca acgctcgtca cgctcgcggc agaggggggc gcgccgaaag tgatcttggt
     7981 gagcggcacg gaacggatcg agacggcgcg cgatcgcgtc acgacgacct cgacgcagga
     8041 gaatgcctgg ctcgtgctcg ccgcgcgcgc tctcggcaag caaagcgtga cccttaccgt
     8101 caacgacggc gccgaggatg ggccgctcta tcggagcttc acggagcacg agattgccgc
     8161 cggcccgctg gtcgtgacca atagcggcga tacgccgctc gacgccgtcg tctcggtgag
     8221 cggcgcgccc gtcacgccgg aacccgctgc cgagcacggc ttcacgcttg cccgcagctt
     8281 ccacacgctc gacggcgcag aggccgatct cggcacggcg aagcagaacg accgcttcgt
     8341 tgtggtgctg accgtgaccg agccgcagcc cgagttcgcg cgcgttgcgc tgacggacta
     8401 tgtcccagcc ggcttcgaga tcgacaatcc gcgtctcgtc tcctcgggcg atgcgggcac
     8461 gctcgactgg atcgccaatg ccggcacgcc cgtccacacc gagttccgcg ccgaccgctt
     8521 caccgcggcc ttcgagcggc gcaagggcga cccgaccacc tatagcgtgg cctatatcgt
     8581 ccgcgcggtg tcgcccggaa gctacgttct gccgcaggcg gtggtcgagg acatgtatcg
     8641 gcccgaccgc ttcggccgga ccgacaccgg cgcggtcgag gtcgctccgg cgcgatgagc
     8701 agcagcgcga aaacgagccg ttggaagcga cgtgcgcttt tcgcggcggt cggcacgatc
     8761 tggctcatcg ccgcggcggc gggcgctctc tccggactgg tcgagactta cgggccgccg
     8821 ccgctcgggc gcgatctcga tgcctcgcgc gtggttctcg accgcaacgg catgctgctc
     8881 agggcctacc tcacgggcga aggccgctgg cggcttgccg ccagccgcaa tgacgtcgat
     8941 ccacgctacc tcgaggcgct cctcgcttac gaagacaagc gctacttcgt gcatgacggc
     9001 ctggatccgc tggccatcgg ccgcgcgttc tctcagctcc tcacccatgg ccgcatcgtc
     9061 tcgggcggtt cgaccctcac catgcaggtc gcgcgtctgc tcgagccccg gcgggagcga
     9121 tcgctggacg caaagatcag acaggcgttg cgcgcggttc aactcgaaag ggcgttgagc
     9181 aaggacgaga tcctcgggct ttacctgacg cttgcgccct atggcggcaa tcttgaaggc
     9241 gtgcgggcgg cctcgcttgc ctatttcggc agggagccgc gccggctcac gctcggccaa
     9301 gcggcgctgg tggtcgcgct gccgcaagcg ccggaagctc gaaggcccga tcgctttcct
     9361 gaagccgcga agcgcgcgcg cgaccgcgtg ctcgaccgga tcgcaggcgc tggcgtgttc
     9421 agcgacgccg agatcgcgcg ggccaagcaa gagaaggtgc cgagcgggcg cgagccgatg
     9481 ccgcttctcg cgccgcatgc cgccgatcag gcgctcaagg acacgccgca ggagcgcgcc
     9541 ttgcggctca cgatcgacgg gcgactgcag aaggctctgg agcatctcgc gcgcgaccgg
     9601 gcgcgagcgt tgggcccgga catgtcggtg gcgatcgtcg tggtcgacaa tgcgaccggc
     9661 gaagtcttgg cgcgcgtcgc ttcgcccgat tatttcgacg agcgccgcgc gggccaggtc
     9721 gatctgacgc aagctgtgcg ttcgccgggg tcaacgctca agcccttcat ctacggtttc
     9781 ggcttcgagg acgggttgat ccaccccgag acgctgatcg aggaccggcc ggtccgctat
     9841 gccgggtaca gccctgagaa tttcgacctc accttccagg gcacggtcac ggtgcgccgc
     9901 gctctgcaac tctctctgaa cgtcccggcg gtcgccgtgc tcgaggcgat cggcgcaagc
     9961 cggctgaccg cgcgccttca agaggcgggc gcgttcctcg tgctgccgcg ccgcgaggcg
    10021 ccgggactcg cgctcggcct tgggggcatc ggcatcaggc tcaccgacct caccatgctc
    10081 tatggcgggc ttgcgcggca ggggacggtg gcgccgctcc tcgagcgact gggcgccgcg
    10141 cctctcgcgc cgcggcggct gatcgagccg gccgccgcct ggtctgtcgc caatgtgctg
    10201 gccggcacgc ccccgcccga gagcgcggcc ggagggcgca tcgccttcaa gaccggcacg
    10261 agctacggct atcgcgacgc ctgggcgatc ggcttcgacg gcaagcggac gatcggcgtt
    10321 tgggccggga gacccgatgg cgcgccggtt gcagggctga tgggccggac agcggcggcg
    10381 ccgatcctgt tcgacgcctt cgcgcgggtt ggagggcgtc tgaacccgct gccgccggcg
    10441 ccaaacggcg cgctcatcgc aagcacggcc aaattgccgc cgcctcttca gcgcttcgcc
    10501 tcggccgcaa gcacgggcga ggcgatggcg ccgaaagtac ggatcgtctt tccgcccgac
    10561 ggcgcgagcc tcgaattgtc gggacgcgaa ggagaaccgc gagacccaat cgccatcaag
    10621 attgccggcg gcactccgcc tctcagcgtg ctcctcaacg gcatgccgct cgccggcaag
    10681 aaatcggcgc gaacgctgtt cttcgcgccg gacggcccgg gcttcgtgcg gctcaccgtg
    10741 accgacgcga agggcacggc cgacagcgtc gtggtgcggc ttcagtaagg tcgcacccgg
    10801 attcacgccc tggccttgtc gcgcctctta gcctaaaagc gcgaagctcg gatcgggagg
    10861 gacgctcgca tgtctcgaag cgcggccagt cgcgatggaa aatttcgtgg cggcccgctc
    10921 attccggtct tgtttttcgc aaccccgctg ttgacggcgg tcgctccccg cctcgcgccg
    10981 ttcctgcttc tgatccttgc gctcgtgctg atcgttgcgg cactccgccg cggtctcgcg
    11041 tggcgcgcgc tgcttgagcc aaacccggcg atgatggcgc tcgccgtgct cgccgcttat
    11101 gcggtgctga gcgcgatctg ggcggcggaa cccgacgagg cggtctccaa gggtttgctg
    11161 ctgcttgccg tcacgttcgt ggtctttgcc gtcgccgccg caatcccttc gctcgatgcg
    11221 gagcagattc gacgcgccgg gattgccttc gtcgcaggcg cactttgcgc cgccctcttc
    11281 gtcacgatcg aagttctcac gacgggggcg ctgacgcgcg cggcgatgaa tgtcattccc
    11341 gccctgcaac cggacagcgc gaaacgcatc aagatcgtgg acggacgcgt aacctacatc
    11401 aggggggcgg tgttcaatca acatgtcgtc atgctggcgt tccagctctg gccggggttg
    11461 ctcgcgctct ccacactgca aggcccgcgg cgcggacttc tcagcgcgct gttctttatc
    11521 gctcttgccc tgccgatctc actctccgag catgactcct cgcaaatcgg cctgatcgct
    11581 tccgcgctcg tcctgcttct tgcttgggtc tggtcccgcg cggcgatacg cggccttgcc
    11641 gtggtctggt gccttggctt cgcgctcgtg ctgccgctcg cctttctctc ttacaaggcg
    11701 gaacttcatc ttacgaaatg ggtgccccaa tcggcgcggg cgcgaatcat tatttgggaa
    11761 tacaccgccg aacgcgtgtt cgagcgccct tggcttggga tcggtgccga ttcgaccccc
    11821 ggctcgaaag cggcgcctgc gaagctggat aagccgaagg gcttcgtgca gcctcgcacc
    11881 accgggcacc acgcgcacaa tcttttcctg caaacctggt acgagcttgg cttggtcggc
    11941 gcgcttctgg cggcaatcgc gggtgccgcg gtggtgttgc gcattttcct gttgccggag
    12001 cccgctcaag cctatggtgc cgccgccttc accatgttcg cggcgatcgc cgccttcgcg
    12061 tttggcatgt ggcaggtgtg gctgatctct gcggttggcc tgctcgtcgt ttacctgctg
    12121 atggccaccg cctttaccgc ccgggggcgg ggctaaaatg aaggtccccg ccctcattcg
    12181 gactggccct ggcccttgac gaggatggcg cccgtgccca agaacagggc gtggctcaag
    12241 ctcccgaaac accgttggtc agacgcgtcc atatcaagac gttcggctgc caaatgaatg
    12301 aatacgactc cgagcgcatg gccgaggcgc tcggagccga aggctaccag ccaaccgagg
    12361 atccggccga ggccgacctc gtcattctca acacctgcca tatccgggag aaggcggcgg
    12421 agaaggtcta ttctgaactc gggcgcgtgc ggcttgccaa agagcggcgg aagcgtgagg
    12481 gccgcgatac gctcatcgcg gtcgcgggct gcgtcgccca ggccgaagga caggagatca
    12541 tggcgagagc gccggccgtc gatctcgtcg ttggaccgca gagctatcac cgcctcgcaa
    12601 gcctcctcgc cgaggcggca acgagcggca aaggcgtcgt cgccaccgat ttccccgcgg
    12661 aagacaaatt cgcgcagctt cctccgcgtt cgcgacaggc gagccaccct tcggtcttca
    12721 tcaccgtgca ggaaggctgc gacaagttct gcaccttctg cgtcgtgccc tatacgcggg
    12781 gcgccgaata ttcgcgcgcc ccgtccgaga tcgtcgccga ggctgagcgg ctcgcggccc
    12841 aaggcgcgcg cgagatcacg ctgctcggcc agaatgtgaa cgcctatcac ggccaagccg
    12901 ccgatgggcg tccctggagc cttgcgcgcc tgttgcggcg gctcgccgaa ttgccgcggg
    12961 tcgcgcgact gcgctacacc acgagccatc cgctcgacat ggacgacgag ctgatcgcgc
    13021 tcttcgccga agagcccaag ctcatgccgt atctgcacct gccttttcag tccggctcgg
    13081 accgcatcct caaggccatg aaccgcaagc acacgatcgc ggactacgag gcggtgatcg
    13141 gaaaggtgcg cgcggcgcgc cccgatattg cgctctcgac cgacattatt gtcggcttcc
    13201 ccggcgaaac cgaccgcgat ttcgaggaaa ccatggctct tgccgcccgc gcgcgttttg
    13261 cgcaggcctt caccttcaag tatagcgcgc ggcccggcac gcccgcggca agtttctccg
    13321 gacaggtccc cgaggcggta aagcgcgagc gcc
//
LOCUS       DASUQP010000008        11767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1911520, whole genome shotgun sequence.
ACCESSION   DASUQP010000008 DASUQP010000000
VERSION     DASUQP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911520"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1060)
                     /locus_tag="VFY74_00365"
     CDS             complement(188..1060)
                     /locus_tag="VFY74_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930196.1"
                     /translation="MGSDNIATGTGANASGNDGRNIASGANANASGAGSSNIATGRSA
                     DASGSDSDGDDDGSENIATGAGANASGDDSRDIAVGAFANASGDLAQNVAIGANAQAI
                     GGTISGSTAIGANARANFDNSGAFGAGATTTRANQQMFGTASNTYTMAGITSGASRTA
                     QGAPTHLVTSNTGGDLAAHTFAELGLATTGDVAGLQTQINRLGRRDRELTEGIAAVAA
                     LAQPILLPGQHFAVRAGWGGYDDANAVGFSAAGVVASNLLSQGRGTLTLDGGLGFGTD
                     EGEVAGRAGATIGW"
     gene            complement(1198..1779)
                     /locus_tag="VFY74_00370"
     CDS             complement(1198..1779)
                     /locus_tag="VFY74_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930197.1"
                     /translation="MATIAKLVAPLSAATERPRDTPSRGAKAKRRLWVSVSVLSLAVN
                     FNATLSPRPAWAVICENFGAGGDPAGIDGGINTNTACGISANAAGGVLPSQNAAFGFG
                     ANASGSGGSNIAIGNANASGASGDNIATAELPTPAATTAKISQPVSPPTPAALMAMLA
                     GAAAPTSQPASALAPSGTTALTRQSAAISTATA"
     gene            2118..3011
                     /locus_tag="VFY74_00375"
     CDS             2118..3011
                     /locus_tag="VFY74_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5930198.1"
                     /translation="MIGVRKSFQKSMGFGAAPAWFAASLLALALIALWQGPARSQARN
                     DIVPDAWQGDVLRPKPDMQGLAKLRFITDSDYPPFHYVDEVGALTGFNVDLAQAICET
                     LEVECEVKDVDWAELYARLDNGEADAAIASIRISAESLGKADFSSRYYATPARFIAQK
                     TNELKEISPETLEGKKIGVAKNTGHEAYLKQFFPDVALATFDTTEDAERALKDGAIDF
                     VFGDGIGMTFWLNGVTSEGCCEFRGGPYLDTKFFGEGVGIAVKKGNRQLAQILTYALE
                     QVHASGRYEELFLRYFPISFL"
     gene            complement(3015..3875)
                     /locus_tag="VFY74_00380"
     CDS             complement(3015..3875)
                     /locus_tag="VFY74_00380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5930199.1"
                     /translation="MDGLLGQSDGFIRFAIFAGVFLAMALIELLWPKRELLVSKRKRW
                     LTNIGISATGTLLLRLMAATAVPVAAVAAAFYAETHQVGLLNQVACPAWIKIAVALVV
                     LDLAIWAQHWASHKVPLFWRLHQVHHADRDIDVTTAVRFHPVEIGLSMLWKIAVVVPL
                     GAAPLAVFLFEVILNACAIFNHANIAMPAWLDGILRLFIVTPDMHRVHHSILRREHDS
                     NYGFNLSIWDRLFGTYTAEPEAGHQGMTIGLGPYQSEAPTRFGWSLWLPFGQQGSDIR
                     DQKSDIGDQK"
     gene            complement(3951..4799)
                     /locus_tag="VFY74_00385"
     CDS             complement(3951..4799)
                     /locus_tag="VFY74_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007802175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5930200.1"
                     /translation="MPASALPKVKLPSGEAVPQLGQGTWGMGESGRARKEEIAALKLG
                     LDLGLKLIDTAEMYGNGGAEEVVAEAIAGRRDECFIVSKVLPENATKAGTVAACTRSL
                     KRLKTDRIDLYLLHWRGEPKLEETLAGFEALTALGAIRYWGVSNFDIGDMEELFHLPG
                     GSACASNQVLYNLRRRGIEAGLLPWCRERGIPVMAYSPFEQGRLLRERVLTGVAIRHR
                     ANTAQIALAWILRHGDMMVIPKTSTEARVRENHAALGIELTERDLVELAKAFPPPKRR
                     RPLELL"
     gene            complement(4876..5490)
                     /locus_tag="VFY74_00390"
     CDS             complement(4876..5490)
                     /locus_tag="VFY74_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX5930201.1"
                     /translation="MAQSKAASKERCDALSEVLADNWWAVGLRGILGILFGLICLFTP
                     GVAVAVFVIMFAAYMLVDGAFAIVAGIKAARNGERWGLLILEGIVDIAAGLVAVAWPG
                     ITLVALIWLIAIWAIVSGALMLWAAFTLNADHGRWWLALGGIASLIFGILLVLQPLIG
                     AIVLTLWIGAYAFVFGIFLLVLAFQLRTRKQERGRGAPAAAKKA"
     gene            complement(5621..6547)
                     /locus_tag="VFY74_00395"
     CDS             complement(5621..6547)
                     /locus_tag="VFY74_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5930202.1"
                     /translation="MTSIDHANLSRLDLNLLVAFDALLTERSVTRAAARLGLGQSAMS
                     HNLGRLRTLFGDELLTRGADGMRPTPRALELADPVRVTLAQIQAAVLQREAFDPATAE
                     RTFRIGLADSIEVAIIPGLMARLRQVAPGVCLRLRSLNRLTILDELDTGALDLGIGVF
                     DHGQIHHKRRPLYKETFLCLFNPALLNFTPPISLDEYLSVPHVLASLTDDAHGAVDEA
                     LAKLKLKRTIALTTPGFLAVPFVVARAPVVTTMPSRLARYFAESFSLATSPAPVDLPS
                     FAIALLWHGSFDHDPGHIWLRQTVSGVAAEVA"
     gene            7012..7644
                     /locus_tag="VFY74_00400"
     CDS             7012..7644
                     /locus_tag="VFY74_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX5930203.1"
                     /translation="MRKAPFTVCVVGGGFTRAAVAIALMARIRSPFRLVIVERGRTLG
                     RGIAYGSYHPLHLLNVRTRDLSVCASQPGDFLNWAFGQLDQGENQAGLHEALAHTFLP
                     RQLFGEYVRERLNDSVRRRPDVTFSVINDTAISCIADGGCYRIAFEQTAPVPADSVFL
                     ATAYGILSASTKEALKPFEDLPAERLAAARTIALIGSGLTMGRCAAVGPP"
     gene            7794..8420
                     /locus_tag="VFY74_00405"
     CDS             7794..8420
                     /locus_tag="VFY74_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930204.1"
                     /translation="MAEESGTPWQAIINGLRPSLPQIWQGLPIAEQARFLRHLRSVWN
                     AHRHRLPIEVHSRLMAEFTDGRAKLVRGRVSAVARDSDGFTLTLHTRATGDPTSVTAD
                     LAFDCTGFRPDLDQPLIRTLIERDLVCPDPHRLGLVVARNGRTIDGRGNSSPHLFAIG
                     PLCQGTLWEITAVPEIVAEADAAAQEVALLADGQIEAAGQKAAETACS"
     gene            8949..9890
                     /locus_tag="VFY74_00410"
     CDS             8949..9890
                     /locus_tag="VFY74_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930205.1"
                     /translation="MPDQDSGFTSPDQLMDWISNYRDHKQPWRVPGAVHAMNDYGLFG
                     DEEKAWFCIGFIAGVLGTNPKDGPGMIPKMFPMPDKEQAVIIRAIAYSGRPDWRELLE
                     KNASRMPLRRPLIDDFLNGKRPTLMTLPLDTGGSPGVYALWGYYVATGQHEPVVRIME
                     ALRWSKTKDDSGFSFRKVFSGWGSDPTAVDKITTGGTAKWTLASYAERDRQLLALYRA
                     EQGRQPEEIAKPLNDVIEAAELFESEKIRKDQFGAIEDAQRQKLSNEAGMTKGATAGS
                     IAIATGCVAASALGQAYIALPCVIGGALYTGAVKLAH"
     gene            complement(9900..10760)
                     /locus_tag="VFY74_00415"
     CDS             complement(9900..10760)
                     /locus_tag="VFY74_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930206.1"
                     /translation="MNRKPSESRIDRGERFIWKDGEIEITPAPGKAMPGLASSGLPWP
                     EFVYRLIEHYKWEPQHLRGTVAEFDTRMRRQEVPLNFLFNILLRWLSSDAIKGLLNRF
                     CPANDVSGPLGVERLWEAKFAQPDVRIESPCSRIFIEVKVDAVTKLEQLQRFLLLHAE
                     MNARFGEKRPYLFFLTKNGLSKCWQPRGELKSDVNVQDSLRRLSEVALPESLGRRALK
                     EASASYEDVRTRITYGAATWSAIGMFLRELGINVGDSINRRAIEDFVSELHKRGLFED
                     RILASGRSTE"
     gene            complement(10916..11203)
                     /locus_tag="VFY74_00420"
     CDS             complement(10916..11203)
                     /locus_tag="VFY74_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930207.1"
                     /translation="MFSLAGAERKAARLLKGNFDLGDLYDQLEALRDSVQALSTRAGQ
                     GASRGYARTRDLAADTWDDAEDAMKDHLAASLLLAVGLGIVVGYFIRRGTE"
BASE COUNT         2153 a         3807 c         3668 g         2139 t
ORIGIN      
        1 aaaccgtcat gcccggactt gatccgggca tccattccgt gaccccaggc caagctgagg
       61 cgtaagggaa tggacccccg ggtcaagccc gggggtgacg aggaacacac aacatccttc
      121 gtgctgagga gcgcgcggcg actcgcctgg caacgcggtg cgagttcccg tatgacgccc
      181 agacccgcta ccagccgatg gtcgcgccgg cgcggcccgc cacctcgccc tcatcggtgc
      241 cgaagcccaa gccgccgtcg agcgtgagcg tgccgcgccc ctggctcagg aggttgctcg
      301 cgaccacgcc cgcggccgaa aagccgacgg cattggcgtc gtcatagccg ccccagccgg
      361 cgcggacggc gaaatgctgt ccgggcaaga ggatgggctg cgccagcgcc gcgacggcgg
      421 cgatgccttc ggtcagctct ctgtcccggc gcccgaggcg gttgatctgc gtctgcaggc
      481 ccgccacgtc gcccgtcgtc gcgagcccga gctcggcgaa ggtgtgggcg gcgagatcgc
      541 cgcccgtgtt cgaggtcacg agatgggttg gcgccccttg cgccgtcctg ctcgcacccg
      601 aagtgatccc ggccatggtg taggtgttgc tcgccgtgcc gaacatttgt tgattggcac
      661 gcgtggtggt ggcgccggcg ccgaacgcgc ccgaattgtc gaaattggcg cgggcgttcg
      721 cgccgatggc ggtactgccg ctgatggtgc cgccgatggc ttgtgcgttg gcgccgatgg
      781 cgacattctg agcgagatcg ccgctggcat tggcgaaagc gccgacggcg atgtcgcggc
      841 tgtcgtcgcc gctggcattc gcgccggcac ccgtggcgat attctcgctc ccgtcatcgt
      901 cgccgtcgga atcgctgccg ctggcgtcgg cggaacggcc ggtcgcgata ttgctgctgc
      961 cggcgccgct ggcgttggcg ttggcgccgc tagcgatgtt acggccgtcg ttgccgctgg
     1021 cgttcgctcc ggtacccgtg gcgatgttgt cgctacccaa cacgctcccg agcgggccat
     1081 cggtgccgct ggcattggcc gaaaagccgg tcgcgatgtt gcggctgtcg tcgccgctgg
     1141 cattggcgtc ggcgccggtt gcggtgttga tgctgctgtc gccgctggca ttggcgccta
     1201 cgccgtcgcc gttgaaatcg ctgccgattg ccgtgttaga gctgtcgttc ccgaaggcgc
     1261 cagcgcggag gccggttgcg atgttggcgc tgccgctccc gctagcatcg ccatcagtgc
     1321 cgctggcgtt ggcggagaga ccggttgcga tattttggct gttgtcgccg ctggcgttgg
     1381 cagctcggcc gttgcgatgt tgtcgccgct ggcgccgctg gcattggcgt tgccaattgc
     1441 aatgttgctg ccaccgctgc cacttgcgtt ggcgccgaag ccaaatgcgg cgttttggct
     1501 aggcaaaacg ccaccggcag cgttggcgct aatgccgcag gctgtgttgg tgttaatgcc
     1561 cccgtcgatt cctgccggat ccccaccggc accgaaattt tcacaaatga ccgcccaggc
     1621 cggccgcgga gacaacgttg cattgaaatt gaccgccaat gagagcacgc tcaccgacac
     1681 ccagaggcgc cgcttcgcct tcgctccccg cgacggcgta tcacgcggac gttctgtcgc
     1741 tgcgctcaaa ggcgcaacaa gtttcgctat ggtggccata tccccctccc gccgtatgag
     1801 cccgcgaggg accttgggat cccgcgcacg aggccgattc acccgaggcg taagtttaat
     1861 gccgtctgga agggtgcggt tgttgcaatc ccaacgcaca tgccaatttc tgtttgcact
     1921 gtgtctggtg agccacagtc aagatgatga attcgatcgc gcagcaggcg cgtgcatgtt
     1981 gctgaccttc cacaagatcg agacctctgg ggtaagcaca caaagtggcg ccggaattca
     2041 gggagcaggg ctgcgtcacg caggggggcg cttggctgcc cttggcagcg cccatcgtgc
     2101 cgcgttaaga caggcgcgtg atcggcgtgc gcaagagttt ccagaaaagc atgggtttcg
     2161 gcgcggcacc cgcgtggttt gccgcttccc tccttgccct cgcgctcatc gcgctttggc
     2221 aaggcccggc gcgttcgcag gcccgcaacg acatcgttcc cgatgcctgg caaggcgacg
     2281 tgctccgtcc caagcccgac atgcagggac tggcgaagct gcgcttcatc accgacagcg
     2341 actatccgcc cttccactat gtcgatgagg tcggtgccct caccggcttc aatgtcgatc
     2401 tcgcccaggc catttgcgag accttggagg tcgagtgcga agtgaaggac gtcgattggg
     2461 ccgagcttta cgcccgtctc gacaacggcg aggcggacgc ggccatcgcc tcgatccgca
     2521 tcagcgccga gtcgcttggc aaggccgact tcagctcccg ctattacgcc acgcccgccc
     2581 gcttcatcgc ccagaagacg aacgagctta aggagatcag ccccgagacg ctcgaaggca
     2641 agaaaatcgg cgtcgccaag aacacagggc atgaggccta tctcaagcag ttcttccccg
     2701 atgtcgcgct cgcgaccttc gacaccaccg aggacgctga gcgggcgctg aaggacggcg
     2761 ccatcgattt cgtgttcggc gacggtatcg gcatgacgtt ctggctgaac ggggtcacca
     2821 gcgaaggctg ctgcgagttt cgcggcgggc cctatctcga taccaagttc ttcggcgagg
     2881 gcgtcggcat cgcggtgaag aaggggaatc gccagctcgc ccagatcctc acctacgcgc
     2941 tcgagcaggt gcatgcgagc gggcgctacg aagagctgtt cctgcgctac ttccccatca
     3001 gctttctctg aaatctattt ctgatctccg atatctgatt tctgatctct gatatctgat
     3061 ccctgctgcc cgaagggcag ccaaagcgac cagccgaacc gcgtcggcgc ctcgctctga
     3121 tagggcccga gcccgatcgt catgccctga tgcccggcct caggctctgc cgtataggtg
     3181 ccaaacagcc ggtcccagat cgagaggttg aagccgtaat tcgaatcgtg ctcgcggcgg
     3241 aggatcgagt ggtgcacgcg atgcatgtcg ggcgtcacga tgaagagccg caaaatgccg
     3301 tcgagccacg caggcatcgc gatattggcg tgattgaata tggcgcaggc gttgaggatc
     3361 acctcgaaca ggaacacggc aagaggggcg gctccgagcg gcaccaccac cgcgatcttc
     3421 cacagcatcg acagcccgat ctcgaccgga tgaaaccgga ccgccgtggt cacgtcgatg
     3481 tcgcggtcgg cgtgatgcac ctgatgcagg cgccagaaca gcgggacttt gtgcgacgcc
     3541 caatgctgcg cccagatggc gaggtcgagc acgacaagtg cgaccgcgat cttgatccag
     3601 gccggacatg cgacctggtt caggagcccg acttggtgcg tctctgcgta gaaggcggcg
     3661 gcgacggcgg cgaccggcac cgccgttgcg gccatgagcc tcagcaggag cgtgccggtc
     3721 gcgctgatgc cgatattggt gagccaacgc ttccgctttg agaccaggag ctcgcgcttg
     3781 ggccacagca gctcgatcag cgccatcgcc aggaacacgc cggcgaagat ggcgaagcgg
     3841 atgaacccgt cgctctggcc gaggagaccg tccatggccc gatgttagcg tgtaacgcgg
     3901 cgcgcgtcag tgcgtcggcc tggcggcgac gccgtgatga cagcgagcgc tcacagcaat
     3961 tcgagcggac ggcggcgctt cggcggaggg aaggccttgg cgagctcgac gaggtcgcgc
     4021 tcggtcagtt cgatgccgag cgcggcgtgg ttctcgcgca cccgcgcctc ggtcgaggtc
     4081 ttggggatca ccatcatgtc gccatgccgg aggatccacg cgagtgcgat ctgcgccgtg
     4141 ttggcgcgat ggcggatcgc cacccccgtc aacacgcgct cgcgcaacag ccgtccctgc
     4201 tcgaagggcg aataggccat gaccgggatg ccgcgctcgc ggcaccaggg aagaagcccg
     4261 gcctcgatgc cgcggcggcg caaattgtag agcacctggt tgctcgcgca ggcgctgccg
     4321 cctgggaggt gaaacaactc ctccatgtcg ccgatgtcga aattgctgac gccccaatag
     4381 cggatcgctc ccaatgctgt cagcgcctcg aagccagcaa gggtctcttc gagcttcggc
     4441 tcgccgcgcc aatgcaggag atagaggtcg atgcggtcgg tcttcagccg cttcaggctg
     4501 cgcgtgcagg cggcgacggt tccagccttg gtcgcattct ccggcagaac tttgctcacg
     4561 atgaagcatt cgtcacgccg ccccgcaatc gcctcggcca cgacttcctc cgcgccacca
     4621 ttgccgtaca tctcggcggt gtcgatcagc ttaagcccga gatcgaggcc gagtttgagc
     4681 gcggcgatct cctccttccg cgcccgcccg ctctcgccca tcccccatgt gccttggccg
     4741 agctgcggca ccgcctcgcc gcttggcagt ttgaccttgg gaagcgctga cgccggcatg
     4801 agggaaactc gagaacggag aacgcggcgc ttcatggcgc cgcgcccgat cataacgcgt
     4861 gggaaaggga agcgtttagg ccttctttgc cgcagcgggc gcgccgcggc cccgctcctg
     4921 cttgcgcgta cggagttgga aggccagcac gagcaggaag atgccgaaca cgaaggcata
     4981 ggcgccgatc caaagcgtca ggacgatcgc tccgatcaac ggctggagaa cgaggaggat
     5041 gccgaagatc agggaagcga tgccgccgag cgcaagccac cagcggccat gatcggcgtt
     5101 cagtgtgaag gccgcccaca gcatgagcgc ccccgatacg atcgcccaaa tcgcgatgag
     5161 ccagatcaag gccacgagcg tgatccccgg ccaagcgacg gcaacgaggc cggccgcgat
     5221 gtcgacgatc ccttcgagga tgagcaggcc ccaacgctcg ccgttgcgcg ccgccttgat
     5281 gccggcaacg atggcaaacg cgccgtcgac cagcatatag gccgcgaaca tgatgacgaa
     5341 gactgcgacc gccacacccg gcgtgaacag gcagatcagg ccgaacagga tcccgaggat
     5401 cccgcgcaaa cccaccgccc accaattgtc agccaagacc tcgcttagcg cgtcgcagcg
     5461 ctcctttgat gccgctttgc tttgagccat gaaagaacct ccttcgatcg atccgctaaa
     5521 cccgctcgaa cctttgccaa gcgattcgaa tagcacgcaa tcatgaagcc ttatcggcaa
     5581 gacggtaaaa ggaatttgtc ccaacccggc gcaagcagcg ctaggcaacc tcggccgcca
     5641 cgccggaaac ggtttgccga agccagatat ggcccggatc gtgatcgaag cttccatgcc
     5701 acagaagcgc gatggcgaaa ctcggcagat cgaccggcgc gggactggtt gcaagcgaga
     5761 aactctccgc gaaatagcgg gcgagccgcg acggcatcgt ggtcacgacg ggcgcccgcg
     5821 ccaccacgaa cgggacggcg aggaagccgg gcgtggtcaa agcgatggtc cgcttgagtt
     5881 tgagctttgc aagcgcttcg tcgaccgcgc cgtgggcgtc gtcggtgagg ctcgcaagaa
     5941 cgtgcggcac gctcaaatat tcatccagcg agatcggcgg tgtgaagttc aagagcgccg
     6001 gattgaacag gcagaggaag gtctccttgt aaagcggccg gcgcttgtga tggatttgcc
     6061 cgtgatcgaa cacgccgatg ccgagatcga gcgctccggt gtcgagctcg tccagaattg
     6121 taagccggtt gagcgagcgc aggcgcaggc agacgcccgg cgcaacctgg cgcagtcgcg
     6181 ccatgagacc cgggattatg gcgacctcga tggagtcggc gaggccgatg cggaaggtcc
     6241 gctcggcggt cgcaggatcg aacgcctcgc gctgaagcac cgccgcctgg atctgggcaa
     6301 gcgtcacccg caccgggtcg gcaagctcga gcgcgcgcgg cgtgggccgc atgccgtcgg
     6361 cgccgcgcgt cagcagctca tcgccgaaca gcgtcctgag ccgtccgaga ttgtggctca
     6421 tggccgactg gccgagccca aggcgcgccg cggcgcgtgt gacgcttcgc tccgtgagca
     6481 gagcgtcgaa cgcgacgagc aggttcaggt cgagcctgct gagattagcg tggtcgatac
     6541 tcgtcattga tgtcatcgat tggactgatg ataaacgaga gcgcatgctg tcggcaagca
     6601 gtgtcggcca cgaggagaag cgtagcgagg acgcaacctg cgaaaaggcc aaagcgttgc
     6661 ggcatgcctt gcgagaccca ggagatgccg gacctgaggg agacatgcgc tccccgcggc
     6721 atcgcggaga ttttccctag cgtgtaaagc gcaccggccg cccttccgcc acgcgggacg
     6781 gaggggtggc cggctctgtt tcacgtcagg agtatcattt gcgaattttg tcacatcacc
     6841 gatgaatgcg cccacgactg gcggcgcttc cgttattccc ttcctcgccg cgctgccgat
     6901 tttgaaacca gttgcctccg gcctgcagtg ccccggcatc ggggctccta ggcgccgcaa
     6961 accttgtgta tgacggcgca tacatcggac cgagcctgag gccaactcga gatgcgaaag
     7021 gcacccttca ccgtgtgcgt ggtcggtggc ggcttcaccc gcgccgccgt cgctattgcc
     7081 ttgatggcgc gcatccgctc gccgtttcgg ttggtgatcg tcgagcgggg ccgcaccctt
     7141 gggcgcggca tcgcctatgg cagctatcac cctctgcacc tgctcaacgt gcggacccgc
     7201 gatctatccg tgtgcgccag ccaacccggc gactttctga attgggcctt cggtcagctt
     7261 gatcaaggag agaaccaagc gggcttgcat gaagcccttg cacacacctt cttgccgcgg
     7321 cagttgttcg gcgaatatgt gcgcgagcgc ctcaatgaca gcgtcaggcg aagacctgac
     7381 gtcacattca gcgtcatcaa cgacacggcg atatcctgca ttgccgatgg cggatgctac
     7441 cgtattgcct ttgagcagac cgctccagtc cctgccgaca gcgtattcct cgccacggcc
     7501 tatggcatcc tatcggcttc gaccaaagaa gccttgaagc ccttcgagga cctgccggcc
     7561 gagcgcttgg cggccgcgcg gacgatcgcc ttgattggct cgggcctcac catgggtcga
     7621 tgtgctgctg tcggcccgcc gtgacggctt tcatggcgag gctcttgtga tctcgcgccg
     7681 cgcgcaattg ccgcggccgc atgcgcccca aggcgtggtg ccgaaacgaa tcgacctgcc
     7741 gcgctcaaaa acgtgtctcc ctactggcaa cggcggcccg cattgcctgc gagatggccg
     7801 aagagagcgg cacgccatgg caagcgatca tcaacggtct gcgcccttct ttgccgcaga
     7861 tctggcaggg ccttcccatc gccgagcagg cacgttttct gcgtcacctc cgctccgtct
     7921 ggaatgctca tcgccatcgt ctgcccatcg aggtgcatag ccgtctcatg gctgagttca
     7981 ccgacggccg ggccaagttg gtgcgcggca gggttagcgc ggtggcccgg gacagcgacg
     8041 gtttcacact gacgttgcac acgcgcgcaa ccggtgaccc aacaagcgtc acggccgatc
     8101 tcgccttcga ttgcacgggc tttagacccg atctcgacca gcccttgatc aggactctga
     8161 tagagcgcga tcttgtctgc cccgaccccc atcgtctcgg ccttgtcgtc gcgcgcaacg
     8221 gccggacgat tgacgggcgg ggcaattcgt cgcctcacct gtttgccatc ggccccctct
     8281 gccaagggac gctttgggag atcacagcag tgcctgagat cgtggccgag gccgacgcgg
     8341 ccgcgcaaga ggtcgcgctc ctcgccgatg ggcaaatcga ggctgcaggc caaaaagcgg
     8401 cggaaacggc ctgctcgtga caggaggcaa cggcgtcaat cttccgcatg cttgacggcc
     8461 gcggccctga aaaagccacg aaaccactga aaatcgacgt cctacgggtt ggcacacccg
     8521 agggtactag ggctaacctt gacttcgtca gcacttctgg ggccgggggg caggaagctg
     8581 atgacatgcg tctccacgcg ctcattgcgc tcgcctccct catcgcactc gcgctgcctt
     8641 gcgcggctga gaccgtagag ccgccgcgca cgagctcggt atcgaagcaa ggtgcccctc
     8701 aacttcaggc cccttctctt ctggcgccgc cacagatcca gccctcctcg cctgcgccct
     8761 ctcaagcgtc gcccgctccg tcccaaaagc catcgtcagc gcccgcggtt caggctgcga
     8821 ccccgccgac cgaagtgaag actgaatcgt atttgcgtcc ggccgagccg acggtgccgg
     8881 cagcttccgt cgtgcctcaa gcgccggctc cgacagcgcc gccaacaacg aacgaccagg
     8941 ctcaagcctt gcccgatcag gacagcggct tcacctcgcc cgaccagctc atggattgga
     9001 tctcgaacta tcgcgaccac aagcagccgt ggcgcgtgcc gggcgccgtt cacgccatga
     9061 acgactatgg cctgttcggc gacgaagaga aggcatggtt ctgcatcggc ttcatcgccg
     9121 gcgtgctcgg gacgaatccg aaggacggtc ctggcatgat cccgaagatg ttcccgatgc
     9181 ccgacaagga gcaggccgtg atcatcaggg cgatcgccta ttcgggccgc cccgattggc
     9241 gcgagctgct cgagaagaac gcgtcgagga tgccgctccg ccggcccctg atcgacgact
     9301 ttctcaatgg caagcggccg acgctgatga cgctgccgct cgataccggc ggctcgcccg
     9361 gcgtttatgc gctgtggggc tattacgtcg ccaccggcca gcatgagccc gtggttcgca
     9421 tcatggaggc cctgcgctgg tcgaagacca aggacgatag cggcttctcg ttccgcaagg
     9481 tgttctcagg ctggggcagc gacccgaccg cggtcgacaa gatcacgacc ggcggcacgg
     9541 cgaaatggac gcttgcctcc tatgccgaac gggaccgtca attgctcgcg ctctaccgcg
     9601 ccgagcaagg gcgtcagccc gaggagatcg caaagccgct caacgatgtg atcgaggccg
     9661 cggagctttt cgaatcggag aagatccgca aggatcaatt cggcgccatc gaggacgcgc
     9721 agcggcagaa gctgagcaac gaagcgggaa tgaccaaggg tgcgaccgcg ggctcgatcg
     9781 ccatcgccac gggttgcgtt gcggcctcgg ctctcggcca ggcctatatc gcgcttccct
     9841 gcgtgatcgg cggcgcgctc tacaccggag ccgtcaagct cgcccactga gccttagcgc
     9901 tactccgtgc tgcggcccga tgcaagtatc cgatcttcga aaaggccgcg cttatgtaac
     9961 tcgctcacaa aatcttcgat cgcgcgccgg tttatggagt cgcctacgtt tatgcccagt
    10021 tcgcggagaa acattcctat agcgctccat gtcgcagccc cgtacgtaat cctcgtgcga
    10081 acatcttcat aactcgcaga ggcttccttt aatgcccttc gacctagcga ctctggcaga
    10141 gcgacctcgg acagtcggcg caaagaatcc tgcacattaa cgtccgattt gagttcacct
    10201 ctaggttgcc agcattttga aagtccattc tttgtgagaa aaaagaggta aggacgcttc
    10261 tcaccgaacc gcgcgttcat ttctgcatgg agtaatagaa agcgctgtag ctgttcaagt
    10321 ttggtcacgg cgtcgacttt gacctcaatg aagattctcg agcagggaga ttcaatgcgc
    10381 acgtctggct gcgcgaattt ggcttcccac agacgttcga cgcccaaggg tccgctaacg
    10441 tcgttggctg gacaaaacct attcagtagc cctttgatcg cgtccgatga gagccatcgg
    10501 agtaggatgt tgaacagaaa gttcaggggt acctcttgac gccgcatccg ggtgtcaaac
    10561 tcggccaccg ttcctctgag atgttggggc tcccatttgt agtgctcgat taagcgatat
    10621 acgaactcag gccacggcaa accgctgctt gccaaccctg gcatggcctt gccaggagcc
    10681 ggcgtaattt caatttctcc atctttccaa atgaatctct ccccgcggtc gatcctgctt
    10741 tccgatggct ttctattcat gcgattaccc tccccacgcg caactgaatc cactagacct
    10801 gagcggtcgg gggagagtca atttctaaga tattagtcct cgcagagcga agtccgctcg
    10861 tgatcggcgg cgcgttctac accggcgcgt caagcctgcc cactgagcct tcgcgctact
    10921 ccgtgccgcg gcggatgaaa tagccgacca cgatgccgag cccgaccgcg agaaggagcg
    10981 aggcggcgag gtggtccttc atcgcgtcct cggcatcgtc ccaagtgtcc gccgccagat
    11041 cgcgggtgcg ggcgtagccg cggctcgcgc cttggcccgc gcgcgtggag agcgcctgca
    11101 cggagtcgcg caacgcctca agctggtcat agagatcgcc gagatcgaaa ttgcccttga
    11161 gcaaccgcgc cgccttgcgc tcggctccgg ccaaactgaa catgacattt acctccttca
    11221 cgattcgggc ttcgcggccg gccgctaagc agctcgcgcg ccgcgccaca agcctttact
    11281 ttaagagaaa cgggcaaggc cgcctctcgt tccaacccgt cttgcgttga tggtgccccg
    11341 cgcgaagctt gcagcagtcg ccattctcct cgtatttgct tcggccggcc ttcttctatg
    11401 gacgccggac acgccacgcg ccggagaccg aagcgagatg ttctcgctaa ggcgggaacc
    11461 ttctcggaga ttggagcggg cgatggggat cgaacccacg acccgtagct tgggaactct
    11521 cagtaccatc gtgcacatcg ccaaatcctt cgtttttctg atggtgaatg aaactaaacg
    11581 ggtcgagaac attctcctat tacgcaccca ttctccaccc agggggccgg gatccgattg
    11641 cctccgctgc aattcgcatg aactctggat ggtggtggcc ataagtctgc tcgagcgtct
    11701 tgaccgacat ccctaggaag ccagctgcct cccgcattga cacacgcttc tgcatgagcc
    11761 acgccat
//
LOCUS       DASUQP010000009         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1366629, whole genome shotgun sequence.
ACCESSION   DASUQP010000009 DASUQP010000000
VERSION     DASUQP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366629"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VFY74_00425"
     CDS             <1..136
                     /locus_tag="VFY74_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087151.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX5930208.1"
                     /translation="WRGPAPPCGTRAYARHVADFIAGMTDRFALAEHRRLFDATPDLR
                     "
     gene            164..1924
                     /gene="argS"
                     /locus_tag="VFY74_00430"
     CDS             164..1924
                     /gene="argS"
                     /locus_tag="VFY74_00430"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5930209.1"
                     /translation="MDVFAGFQGRVLAAVQQLKHDRVVPPDASIEGITLEPPKDASHG
                     DLATNAAMVLAKAAGLPPRALAERIAPLLASDQHIDKVEIAGPGFINLTLASAFWPSV
                     LRMVLEQGAAYGRSAVGKGQAVNVEYVSANPTGPMHVGHCRGAVFGDALANLLAFTGF
                     AVTREYYVNDAGAQVDALAHSAYLRYREALGENIGAIPEGLYPGDYLKPVGETLAAQH
                     GRELLGMPESQWLPIARAASLQAMLAMIEDDLHALGIRHDVFFSERSLSEGPRDAVAE
                     TIADLEAKGLIYRGRLPPPKGKADEDWEDREQLLFRSTAFGDDMDRPLVKSDGTYAYF
                     AGDIAYHRDKFTRGFRTMIDVFGADHSGHVKRMKAAVAALTDGEAELDIKICQLVRLF
                     RAGEPVKMSKRAGKFVTLRELVDEVGAGPVRFMMLYRKNDAPLDFDFVKVTEQSRDNP
                     VFYVQYAHARASSVLRNVRETFPDLDLEEGTLARSDLDRLTDEGEVALIRRIAHFPRL
                     VEAASAAHEPHRIGFYLYDLAGDFHTLWNRGKDLPQLRFIYESDRELTRARVALVAAT
                     KRVLASGLGILGVHALHELR"
     gene            2189..>3265
                     /locus_tag="VFY74_00435"
     CDS             2189..>3265
                     /locus_tag="VFY74_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930210.1"
                     /translation="MFAQMPSYTPVTRGTDPRPQMPEPPQPAYAPYTAQPPQPLHAHP
                     SQHAASGMSYSNPQAPGPMNYPSPEPAPSYQSPEPGYQSAHHASGPGYASAPNYQAAD
                     FGGQSYQPFQPASFRDAEHPGSQPYSASGYDVNQDFQSQSYRDQGFADASFPESSTGD
                     ASYPDASYPETGYDQNFGDTSFTKPGFADAGFSQQQQHGFDASFPDQVFPGAQSASHQ
                     DPHGSDAYGGHDGHSDQGFEPFADEAAPNYGEQVPKSDPRRQLQAFDAIYDQPPQIAL
                     GSTEPARRPAQDYYENERLDADFLDGAQLPPPGTPAKGKLTLRSRSAFMVGSALLGAI
                     ALGGALAFAYKQSGGGLGSEPPPLV"
BASE COUNT          573 a         1114 c         1027 g          551 t
ORIGIN      
        1 ctggcgggga ccggccccgc cttgcggcac acgcgcctat gcccgccacg tcgccgactt
       61 catcgccggc atgaccgatc gcttcgcgct cgccgagcat cggcggcttt ttgacgccac
      121 ccccgatttg cgttagtcgc gccccatatc ctccggagct tccatggacg tctttgcagg
      181 gttccagggc cgggtcctcg ccgcggtcca gcagctcaag catgaccgcg tggtcccgcc
      241 cgacgcgagc atcgagggga tcacgctcga gccgccaaag gacgcatcgc atggcgatct
      301 cgccaccaat gccgccatgg tgctcgccaa ggcggcaggc ctgccgcccc gcgcgctcgc
      361 cgagcgcatc gcgccgctgc tcgcctcgga ccagcacatc gacaaggtcg agatcgcggg
      421 gcccggcttc atcaatctga cccttgcctc cgccttctgg ccgagcgtgc tgcgcatggt
      481 gctcgagcaa ggcgcggcct acggacgcag cgccgtcggc aaagggcaag cggtgaacgt
      541 ggaatacgtc tcggcgaacc cgaccgggcc catgcatgtc ggccattgcc gcggcgccgt
      601 gttcggcgat gcgctcgcca atcttctcgc cttcaccggc tttgccgtga cccgcgagta
      661 ttacgtcaac gacgctggcg cccaggtcga tgcgctcgcg cactcggcct atctgcgcta
      721 tcgcgaagcg ctcggcgaaa acatcggcgc gattcccgaa gggctttatc ccggcgacta
      781 tctcaagccg gtcggggaga cgctcgccgc ccagcacggc agagagcttc tcggaatgcc
      841 ggaaagtcag tggctcccga tcgcgcgagc cgcgtcgctt caggccatgc tcgccatgat
      901 cgaggacgat ctccatgcgc tcggcatccg ccacgacgtg ttcttctccg agcgctcgct
      961 aagcgaaggt ccgcgcgatg cggtggccga gaccatcgcc gacctcgagg ccaagggcct
     1021 gatctatcgc ggacgtctgc cgccgcccaa gggcaaggcc gacgaggatt gggaggaccg
     1081 cgagcaattg ctgtttcgct cgaccgcgtt cggcgacgac atggaccgcc cgctggtcaa
     1141 atcggacggg acctacgcct atttcgccgg cgacatcgct tatcaccgcg acaagttcac
     1201 gcgcggcttt aggaccatga tcgacgtgtt cggcgccgac catagcggcc atgtcaagcg
     1261 catgaaggcg gcggtcgcgg cgctgaccga cggcgaggcc gagctcgaca tcaagatttg
     1321 tcagcttgtg cgcttgtttc gcgcgggcga gcctgtcaaa atgtccaagc gtgccgggaa
     1381 gttcgtcacg ctgagggagc tcgtggacga ggtgggggcc ggccccgtcc gcttcatgat
     1441 gctctatcgc aagaacgacg cgccgctcga tttcgacttc gtcaaagtca ccgagcagtc
     1501 gcgtgacaac ccggtcttct atgttcagta cgcccatgca cgggcgagtt ccgtcctgcg
     1561 taacgttcgg gagaccttcc cggatctcga tctcgaggag gggacgttgg cgagaagcga
     1621 tcttgaccgg ctgaccgatg agggtgaggt cgcgctgatc cggcgcatcg cccattttcc
     1681 gcggctggtc gaggcggcct cggccgcgca tgagccgcac cgcatcgggt tttacctgta
     1741 tgaccttgcc ggggacttcc acaccctgtg gaaccgaggc aaagacttgc cacaattacg
     1801 atttatttac gagagtgacc gagagttaac gcgtgctcgg gtggccctcg tggcggctac
     1861 gaagcgcgtc ctggcgtcgg gtttggggat cctcggcgtt cacgccttgc acgagttgcg
     1921 ctgagccaat ccggggcagg ggggatggtt cgagggaagt atcatgtcga ggcaagatga
     1981 cggattcggc cgcaaggccg gtcctaaccc tgggtcggaa tctgatttca ccaatcggcc
     2041 gagtgtgagc ggcacccgaa acgcacagag ttggggcacc aacgcgctca atgctcagcc
     2101 aagcgaggta ggcttaatga ctcaaggagc gggaaggccg ggctggccgg ccaccggaca
     2161 gcgcccgatg tttggtgggg gcagccccgt gtttgcacag atgccgtcct atacgcccgt
     2221 cacccgcggc accgatccgc gcccgcagat gcccgagccg ccccagccgg cctatgcgcc
     2281 ttacacggcc caaccgcccc agccgctcca cgcgcatccg agccagcacg ccgcttcggg
     2341 gatgagctat tcgaacccgc aagcgccggg tccgatgaac tatccaagcc cggagcccgc
     2401 cccgagctac cagagccctg agcccggtta tcaaagcgcc caccatgcga gcggacccgg
     2461 ctatgcctcc gcgccgaact atcaggcggc ggattttggc ggtcagtcat atcaaccgtt
     2521 tcagccggcc agcttccgcg acgccgagca ccccgggtct cagccctatt cggcttcggg
     2581 ttatgacgtg aaccaggact tccagagcca gtcatatcgg gaccaaggct tcgccgatgc
     2641 gagcttcccc gaatcgagca cgggcgatgc gagctatccc gatgcgagct accccgaaac
     2701 gggctacgac cagaacttcg gggacacgag tttcaccaag cctggcttcg ccgacgcggg
     2761 tttctcgcag caacagcagc acggcttcga cgcaagtttc ccggaccagg tctttcccgg
     2821 cgcccagtct gccagccatc aggatcccca tggttccgac gcgtacggcg ggcatgacgg
     2881 ccattccgat cagggcttcg aacccttcgc cgacgaggcc gcacccaact atggcgagca
     2941 ggtgccaaaa agcgacccga ggcggcagct ccaggccttc gacgcgatct acgatcagcc
     3001 gccgcagatc gcgctgggtt cgacggaacc cgcgcgccgc ccggcgcaag attattacga
     3061 gaacgagcgt ctcgacgccg atttcctcga cggagcgcag cttcctccgc ccggcacgcc
     3121 ggccaagggc aagctgacgc tcaggagccg gagcgccttc atggtcggaa gcgcgctgct
     3181 cggcgccatc gctctcggcg gcgcgctggc ttttgcttac aagcaaagcg gaggcggcct
     3241 cggcagcgag ccgccgccct tggtc
//
LOCUS       DASUQP010000010        11273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1912458, whole genome shotgun sequence.
ACCESSION   DASUQP010000010 DASUQP010000000
VERSION     DASUQP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912458"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFY74_00440"
     CDS             <1..742
                     /locus_tag="VFY74_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5930211.1"
                     /translation="VFDARQYLMGLADGLPAAAAASALAAWLVGAAVLSMRGHYFAIC
                     TLGLGIAAGQIAAGIPWIGGGSGMLVPNPPESLGDVGLLLYYLALVLALGCALTFAWL
                     LSTRFGLALNAIRDDEDKAEAMGIRTHASKVTAWVIAAAFLGAAGAIYGNLVRFIDPL
                     ETAFAGSTIGVWMILMVLLGGKGTLIGPIIGAVVFQITMELFWTYLLGWQRVALGLLI
                     VAVAVFFPAGIVGFVRERKREAEQETRA"
     gene            739..1482
                     /locus_tag="VFY74_00445"
     CDS             739..1482
                     /locus_tag="VFY74_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5930212.1"
                     /translation="MTPLLELADVTKSFGGILANDRVSLRVEPGSIVGLIGPNGSGKT
                     SLFNAVTGVFPIDSGSIRFRGEDISKLLAGEIARAGIVRTFQQPRIYRAMSVAANMLV
                     SAAIRRTAFADLFRRDRPGTGAKIAELLAFVGLDRKQSIRAGELSFGQQKLLELAVAL
                     VNEPELLLLDEPTAGINPVLIERLVDRLKTVNREQGLTLLVIEHNMPVIMGLADHIYC
                     LAHGRLLAAGKPQDIRQDPQVLDAYLGAA"
     gene            1482..2270
                     /locus_tag="VFY74_00450"
     CDS             1482..2270
                     /locus_tag="VFY74_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5930213.1"
                     /translation="MAEASVQTEAGAVPIAELDRLAGAAPLLAIDRLNAGYGRMAVLR
                     DFSLRIARGQSLCLVGPNGAGKSTVLNAIYGFADIASGRVSLAGREITTLPASEKLKR
                     ARLAYVLQQGSVFPAMTVEENLLMGGYLLARAADAREAAERVLAGYPALRARRRERAQ
                     VLSGGERRLLEISRALIMDPELILIDEPSIGLEPRAIDMVFEVLRELRDEGGKTLILV
                     EQNARKGLEFAALGYVLVAGRLAKAGRGKDLLADPDIGGLFLGG"
     gene            complement(2272..3354)
                     /locus_tag="VFY74_00455"
     CDS             complement(2272..3354)
                     /locus_tag="VFY74_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5930214.1"
                     /translation="MARRRRTGSGNFARGAFASFWILLGCLSGLYLFNVLTDPTLGGQ
                     VFKLTPAPTGETAPGTASSQPAGQDFAEIQESLRTLSQQVAALNDRLESNEKRDAAPP
                     GPSAAPETSAPTPAPADPADRAESASPQQAPEEPKPAQAEAPKPAEPTQPEPKPAEQA
                     ALDVNRSPTSTPEPAAPPKPSEPPPVAVSPLDELPAELLEQNTRLDATPAPETGEQAE
                     GLATPEPAPEAAPAAGPSVTSTQSQPEPSSPPPETAPPPSTETAALDPIALPPAANDG
                     STRYGIEIGVVAKQDGLRPLWREYLTNHAALVAGLQPRRVLAPDKKWRLIAGPFTSAE
                     EATQACALFKKASRPCEATVYAGDSL"
     gene            3687..5060
                     /gene="glmU"
                     /locus_tag="VFY74_00460"
     CDS             3687..5060
                     /gene="glmU"
                     /locus_tag="VFY74_00460"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5930215.1"
                     /translation="MTNSSALAIVLAAGKGTRMKSERPKVMHRLAGAPLLAHALWAAK
                     AAGLDRLAVVVGPGMDEVGTTAGALDPTLEIFIQAKQLGTADAVKAARRAFEEFPGQI
                     LILYGDTPLLRAETIGKVRAALQAGADLVVIGFETNNPTGYGRLLLDDRGTLVAIREE
                     KDASEEERALTLCNSGIMGFRSSKTLLNLLGRIGNDNAKKEFYLTDAVALARADRLEA
                     RMVEADAEEVLGVNARAELAAAESLMQQRLRDAAMANGATLIAPETVFLSHDTRIGKD
                     VVIEPHVVIGEGVVIEDGATIKSFCYMQDSRIGEGATVGPFARLRPGADLAKDSHIGN
                     FVEIKKSEIGEGAKVNHLTYIGDTTVGPRANVGAGTITCNYDGFRKHRTEIGAGAFIG
                     SNSSLVAPVKIGEGAYIGSGSVISKDVSPDALALSRTPQEERPGWATKMRERRNRKPK
                     SPGNKGT"
     gene            5121..6941
                     /gene="glmS"
                     /locus_tag="VFY74_00465"
     CDS             5121..6941
                     /gene="glmS"
                     /locus_tag="VFY74_00465"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5930216.1"
                     /translation="MCGIVGILGKGPVAEQLVDALKRLEYRGYDSAGVATVENGELGR
                     RRAEGKLRNLEELVTRSPLYGNTGIGHTRWATHGKPIEANAHPHMTDLVSVVHNGIIE
                     NFRALKDELTAQGVTFTSDTDTEVIAHLITRELKAGNDPVRAVYTSLKHLEGAFALAM
                     IFRGYNDLMIVARQGSPLALGYGNGEMFVGSDAIALAPFTDAIAYLADGDWAVLTRQG
                     VAIRDRDGKAVERPMARSLASSLLIDKGNYRHFMAKEIHEQPEVIGHTLAAYVDLATS
                     SVDIPALPFDFANLTRLSLSACGTAFYACLVAKYWFERWARLPVEVDIASEFRYRETA
                     LDPDGAALFVSQSGETADTLATLRYCRAQAQKIVSVVNVRESSIARESDTVLPTLAGP
                     EIGVASTKAFTCQLAVLACLAIAAGKARGAIGPALEAELVRALGEVPRHMATILRDER
                     PYEMLAHHLSKARDVLYLGRGSSYPVALEGALKLKEISYIHAEGYAAGELKHGPIALI
                     DEKMPVIVIAPRDDLYEKTVSNMQEVAARGGGIVLVSDADPASTGCAIAWHLAVPPVH
                     RFVTPLVYAVPVQLIAYHTATFMGTDVDQPRNLAKSVTVE"
     gene            7040..8263
                     /locus_tag="VFY74_00470"
     CDS             7040..8263
                     /locus_tag="VFY74_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5930217.1"
                     /translation="MLPRVCLVAATVLCAAGAVHAQDAGLKSGAASLAAGKYSDAVRQ
                     LSATVNSDNASAGDAAKALYLRGIAYRKIGEPARAAADLGAAIWLGLPEPDRVKALVN
                     RGLAFRAAGLSKEAEAEIASARKVGGSGAVDQLIVEGGGSAEGAASVAAFSTEVRQEG
                     QASASNERRSWFPSFGRGSSETAAPAPQPEPAPTRTASTSPQWSTTTSKPADAAPGAW
                     NTSVAGAEQPSSSASSGNRLTRWFGSRTESSPAAEPEPAPATQTAAAPPQPSAPPSAP
                     PAASTSSSWSTTTESPSADAGGGATKRSWRLFGRTAEAEPAAQAAVQPSASGGYQLQL
                     ANSRSEDEAKALWKKVSQSQGLGGVSPAIEKIDIGNFGTFYSLKIGPFPDKAQSVKVC
                     NALKRSGIDCSPVTL"
     gene            8384..9172
                     /locus_tag="VFY74_00475"
     CDS             8384..9172
                     /locus_tag="VFY74_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5930218.1"
                     /translation="MKTDDKPSSEKTGAAEDPAQDQLTLLGGAPAHSSHLGARLRGYF
                     LTGLIVVGPMAITLYVVWWFINLVDAWVKPLIPNAYLPETYLPFNVPGVGLIVGILGL
                     MLAGALTANLFGRTLLSHGEMMLDRMPVVRGVYRLFKQIFQTIFSNSGSSFKRVGLIE
                     FPRRGLYCIVFLAGDPPVEVDDKIGGGNPLITVFMPNGPNPTTGFIVFVPAREVILLD
                     LAVEDAAKLIVSAGLVGPDQQQEQLRRLARTKDTKPVPEEEPVI"
     gene            complement(10077..10445)
                     /locus_tag="VFY74_00480"
     CDS             complement(10077..10445)
                     /locus_tag="VFY74_00480"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5930219.1"
                     /translation="MNESNKPHVADEKAGAAYPKTAVQFEQIARDTPEAMRALVAKNL
                     AQSREFYDRSKDALEGLLESWERSFGAAGQGAAALNRKVIDIAQRNINSGFDLAKSLA
                     GARSLAEIMELQVRHARSSS"
     gene            10951..11226
                     /locus_tag="VFY74_00485"
     CDS             10951..11226
                     /locus_tag="VFY74_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930220.1"
                     /translation="MIIERPFSVEGATRNILNQFAISLKNGPVGTGKMYAVGMISQRL
                     VAHPARHGVTVLIRHQKPNAVLFEEFLLEAREGHRSILSCCQDYQTF"
BASE COUNT         1952 a         3589 c         3627 g         2105 t
ORIGIN      
        1 cgtgttcgac gcgcgtcagt acctgatggg gctcgcggac ggactgcccg cggccgccgc
       61 ggcgagcgcc ttggccgcct ggcttgtcgg cgcggcagtg ctctcgatgc gcgggcatta
      121 cttcgcgatc tgcacgctcg ggctcggcat cgccgcgggc cagatcgccg ccggcattcc
      181 ctggatcggc ggcggctccg gcatgctcgt gccaaatccg ccggaaagcc tcggcgatgt
      241 gggactgctc ctctattatc tcgcgctcgt gcttgctctt ggctgcgcgc tgaccttcgc
      301 ctggctgctc tcgacgcgtt tcgggctcgc gctcaatgcg atcagggacg acgaggacaa
      361 ggccgaggcg atggggatca ggacccatgc ctccaaggtg accgcatggg tcatcgcggc
      421 tgccttcctc ggcgctgccg gcgccatcta cggcaacctc gtccgcttca tcgatcctct
      481 cgagacggcc ttcgccggat ccacgatcgg cgtgtggatg atcctcatgg tgctcctcgg
      541 cggcaagggg acgctgatcg gcccgatcat cggcgcggtc gtctttcaga tcaccatgga
      601 gctgttctgg acatatctgc tcggctggca acgcgtggcc ctcggtctcc tgatcgtagc
      661 ggtcgccgtg ttcttcccgg ccgggatcgt cggcttcgtg cgcgaacgca aacgcgaggc
      721 cgagcaggag acgcgcgcat gacgccgctg ctcgaactcg ccgacgtgac caagagcttc
      781 ggcggcatcc tcgccaacga tagagtgagc ttgcgcgtgg agccggggtc catcgtgggg
      841 ctgatcggtc ccaacggatc gggcaagacc agcctattca acgcggtcac cggggtcttt
      901 ccgatcgata gcggttcgat ccgcttcagg ggcgaggaca tctcgaaact tctcgcgggc
      961 gagatcgcgc gggccggcat cgtccgcacc ttccagcagc cgcgcatcta tcgggcgatg
     1021 agcgttgccg cgaacatgct ggtctcggcc gcgatccggc gcaccgcgtt cgcggacctg
     1081 ttccgccgcg accggcccgg aaccggggcg aaaatcgccg aactgctcgc tttcgtcggc
     1141 ctcgaccgca agcagagcat tcgggcgggc gagctttcct tcggccaaca gaagctgctc
     1201 gagctcgccg tggctctcgt caacgagccg gagcttcttc tcctcgacga gccgacggca
     1261 ggcatcaatc ccgtgctgat cgaaagactt gtcgaccgcc tcaagacggt caacagggaa
     1321 cagggcctca cccttctcgt catcgagcac aacatgcctg tgatcatggg gctggcggat
     1381 cacatctatt gccttgccca tggccggctg cttgccgccg gcaagccgca ggacatccga
     1441 caggacccgc aagtgctcga cgcctatctc ggagcggcgt gatggctgaa gcaagcgtgc
     1501 agacggaagc gggcgcggtg ccgatcgccg agctcgaccg gcttgcaggg gcggcgccgc
     1561 ttcttgcgat cgaccggctc aacgccggct acggacggat ggcggtgttg cgggattttt
     1621 cgcttcgcat cgcgcgcggc cagtcgctct gcctcgtcgg accgaacggc gccggcaaat
     1681 cgacggtact gaatgccatc tatggcttcg ctgatatcgc gtcgggccgc gtaagcctcg
     1741 ccggtaggga gatcaccacg ctccccgcaa gcgagaagtt gaagagggcg cgcctcgcct
     1801 atgtgcttca gcagggctcc gtgttccccg ccatgacggt cgaggagaat ctgctcatgg
     1861 gcggctatct tctcgcgcgt gcggccgatg cgcgggaggc ggcggagagg gtgctcgcgg
     1921 gctatcccgc gctccgagcg cggcggcgcg agcgcgctca ggtcttgtcg ggcggcgagc
     1981 ggcggcttct cgaaatttcc cgcgcgctga tcatggatcc cgagctgatc ttgatcgacg
     2041 agccgtcgat cgggctcgaa ccgcgcgcca tcgacatggt cttcgaggtg ctgcgagagc
     2101 tgcgcgatga gggcggcaag accctgatcc tcgtcgaaca gaacgcaagg aagggacttg
     2161 agttcgcggc gctcggctac gtgcttgtgg caggccgcct tgccaaggcc ggacgcggca
     2221 aggaccttct cgccgatccc gacatcggag gcctgtttct cggcggctag gtcagagcga
     2281 gtcgcccgcg tagaccgtgg cctcgcaggg ccgcgaggcc ttcttgaaga gggcgcaagc
     2341 ttgcgtcgcc tcctcggcgc tggtgaaggg gccggcgatc agccgccact tcttgtcggg
     2401 tgccagcacc cggcgcggct ggagcccggc gacgagagcg gcgtggttgg tgaggtactc
     2461 gcgccacaac ggccggagcc cgtcctgctt cgccacgacg ccgatctcga tgccgtagcg
     2521 ggtcgagccg tcattggcgg caggcggcag cgctatcggg tccaaggccg ccgtttcggt
     2581 cgaaggcggc ggagccgtct cgggcggtgg ggatgaaggt tccggctgag actgagtgga
     2641 agtgactgac ggtccggcgg cgggagccgc ttccggcgcg ggctcgggcg ttgcgaggcc
     2701 ctcggcttgt tcgccggttt ccggcgcggg cgtcgcgtcg agccgggtgt tctgctccag
     2761 caactcggcc ggaagctcgt cgagcggcga tacggcgacg ggcggcggct ccgatggctt
     2821 cggtggcgcg gcaggttccg gtgtggacgt cggacttcgg ttcacgtcga gcgcggcctg
     2881 ctcggcgggc ttcggttccg gctgagttgg ctcggcgggc ttcggtgctt ccgcctgggc
     2941 gggcttcggc tcctccggag cttgctgcgg cgaggcggac tctgccctgt ccgccggatc
     3001 ggccggagcg ggggtgggag cagacgtctc gggcgccgcg gaaggccccg gcggcgctgc
     3061 gtcacgcttc tcgttgctct cgagccggtc gttgagcgcc gccacttgct gcgacaacgt
     3121 acgcaaactc tcttggatct cggcgaagtc ctggcccgcc ggttgcgaac tggcggtgcc
     3181 tggagcggtc tcgccggtgg gtgccggggt caacttgaag acttgcccgc caagggtggg
     3241 atcggtgagg acgttgaaga ggtaaagccc tgaaaggcag ccaagcagga tccagaacga
     3301 tgcgaatgcg cctcgcgcaa agttccctga gcctgtccgg cgccgtctgg ccatcggttc
     3361 cgaaacctcc ccgtttacga aaatcgggcg gaaaagcgac gccaataagg ccttgcctgc
     3421 gatttgcgcc gcgaatccca ggatgactcg ctcggcgctc ccggtcaatt tccctcagcg
     3481 gcacggaact ggcgtaggag ggttatggcc ctctcttagg gttttggact agacagatcc
     3541 cgattcggga gcttaggggt gccatcccgg cgagtgcccc ttaagagacg agacttgcgg
     3601 ccccaagtgt taccctcttt ttcggcatta cgaggcatgc ttcttgcttc gatcccgaat
     3661 gtccaatcgg taacgacaga aacggcatga cgaatagctc ggctcttgcc atcgttcttg
     3721 cggccggcaa aggcacgcgc atgaagtcgg agcggccaaa ggtgatgcac cggctcgccg
     3781 gggcgccgtt gcttgcgcat gcgctttggg cggcaaaggc ggccggcctc gaccggctcg
     3841 cggtcgtcgt cgggccgggg atggacgaag tcgggaccac ggccggcgct ctcgatccca
     3901 cgctcgaaat cttcattcag gccaagcagc tcgggacggc cgatgccgtc aaagcggcgc
     3961 ggcgcgcctt cgaggagttt ccgggacaaa tcctgatcct ctacggcgat acgccgctct
     4021 tgcgcgcgga aacgatcggc aaggttcgcg ccgcacttca agcaggggcc gatctcgtcg
     4081 tgatcggttt cgagaccaat aatccgaccg gctacgggcg gctccttctc gacgatcggg
     4141 gaacgctcgt cgcaatccgc gaggagaagg atgcgagcga ggaggagcgg gcgctcaccc
     4201 tttgcaattc cgggatcatg ggattccgct cgagcaagac cctgctcaat ctcctcggac
     4261 ggatcggcaa cgataacgcc aagaaggaat tctatttgac cgacgccgtg gcgctcgccc
     4321 gggccgacag gctcgaggcg cgcatggtcg aagccgatgc cgaggaggtc ctcggcgtga
     4381 atgcgcgcgc cgagctcgcc gcggccgagt ccctcatgca acagcgcctc cgcgatgcgg
     4441 ccatggccaa cggcgccacg ctgatcgcgc cggagacggt gttcctcagt cacgacaccc
     4501 gcattggcaa ggacgtggtg atcgagccgc atgtcgtgat tggcgaaggc gtcgtcatcg
     4561 aggacggcgc cacgatcaag agtttctgtt acatgcagga ttcgcgcatc ggcgaagggg
     4621 cgacggtcgg gccgttcgcg cggctcaggc ccggcgccga cctcgccaag gattcgcata
     4681 tcggcaattt cgtcgagatc aagaaatcgg agatcggcga gggcgccaag gtcaaccacc
     4741 tcacctatat cggcgacacg accgtcggcc ctcgcgccaa tgtcggcgcc ggcaccatca
     4801 cctgcaacta tgacggcttc cgcaagcacc ggaccgagat cggcgccggc gcgttcatcg
     4861 gctcgaactc ttcgctggtg gcgccggtca agatcgggga gggggcctat atcggctccg
     4921 gtagcgtgat ctcgaaggac gtgtctcccg atgcgctcgc gctctcgcgg acgccgcagg
     4981 aagagcgtcc cggttgggcc acaaagatgc gcgaacgtcg caaccgaaaa ccgaaaagcc
     5041 cgggaaacaa agggacatag agcgtgaggt atgggacgat gatgtgccga ttggcaagtg
     5101 gcgataggga gtagggaccg atgtgcggca tcgttggcat cctcggcaaa ggtcctgtcg
     5161 ccgaacaact ggtcgatgcg ctgaagcgcc tcgaataccg cggctacgac tccgcgggcg
     5221 tcgccacggt cgagaacggc gagctcggac gacggcgcgc cgagggcaag ctgcgcaatc
     5281 tcgaggagct cgtcacgcgg tcgccgcttt acggcaacac cggcatcggc cacacgcgct
     5341 gggcgactca cggcaagccg atcgaagcca acgctcaccc gcacatgacc gatctggtgt
     5401 cggtcgtgca caacggcatc atcgagaatt ttcgcgcgtt gaaggacgag ctcacagccc
     5461 aaggcgtgac cttcaccagc gacacggaca ccgaggtcat cgcccatctc atcacacgcg
     5521 agttgaaggc cgggaacgat ccggtccgcg ccgtttacac tagcttaaag catcttgaag
     5581 gcgccttcgc gctcgcaatg atcttccgcg gctacaacga tctgatgatc gtggcgcggc
     5641 aggggagccc gctcgcgctc ggctacggca acggcgagat gttcgtgggc tccgatgcga
     5701 tcgcgcttgc ccccttcaca gatgccatcg cctatctcgc cgacggcgat tgggcggtgc
     5761 tcactcggca gggcgtcgcc atccgcgacc gcgacggcaa agcggttgag cggccgatgg
     5821 cccgctcgct ggcatcatcg cttctcatcg acaagggcaa ctatcgccac ttcatggcga
     5881 aggagattca cgagcagccg gaggtgatcg gccatacgct cgcggcatat gtcgaccttg
     5941 ccacgagcag cgtcgacatc ccggcgcttc cctttgattt cgccaacctc acccggctgt
     6001 cgctctcggc ttgcggcacc gccttttacg cctgcctcgt cgccaaatat tggttcgagc
     6061 gttgggcgag gctgccggtg gaggtcgata tcgcctccga gtttcgctat cgggaaaccg
     6121 cgctcgatcc cgatggtgcc gcgcttttcg tctcgcagtc gggcgagacg gccgatacgc
     6181 tggccacgct gcgctattgc cgtgcgcaag cccagaagat cgtttcggtc gtcaatgtgc
     6241 gcgaatcttc gatcgcgcgc gaatccgata cggtcctgcc gacgctcgcc ggtcccgaga
     6301 tcggcgttgc ctccacaaag gccttcacct gccagctcgc ggtgctggcc tgcctcgcca
     6361 tcgccgcggg caaggcgcgc ggcgcaattg ggcccgcgct cgaggcggag ctggttcggg
     6421 ccttggggga agtgccgcgt catatggcga cgatccttcg cgacgagcgg ccctatgaga
     6481 tgctcgccca tcatttgtcg aaggcgcgcg acgttctcta tctcgggcgc ggctcgagct
     6541 atccggtcgc cctcgaaggg gcgctcaagc tcaaggagat ttcctatatc cacgccgagg
     6601 gctatgccgc gggcgagctg aaacacggac cgatcgcgct catcgacgag aagatgccgg
     6661 tcatcgtcat cgcgccgcgc gacgaccttt acgaaaagac cgtctccaac atgcaggaag
     6721 tcgcggcgcg cggcggcggg atcgtgctcg tgagcgacgc cgaccccgcg agcaccggct
     6781 gcgcgattgc ctggcacctg gcggtgccgc cggtacaccg cttcgtcacg cccctcgtct
     6841 atgccgttcc ggtgcagctc atcgcctatc acacggcaac tttcatgggg actgatgtgg
     6901 atcagccgcg aaacctcgcc aaatccgtga cggtggaata gactagagcc tcggatttgc
     6961 ctgatttttc tgattgagca agaacatggc ggataagcgg aagtcattcc gggcgcgccg
     7021 tgttgctttc ggcgggcgca tgcttccgcg cgtgtgcctc gtggccgcga ctgtcttgtg
     7081 tgccgccggc gcggttcatg cccaggacgc aggcctgaag agcggcgccg cctctctcgc
     7141 agccggcaaa tacagcgatg ccgttcgaca gctctcggcg accgtcaact ccgataacgc
     7201 ctcagccgga gatgcggcca aggcgcttta tctccggggg atcgcctatc gcaagatcgg
     7261 ggagcccgcc cgcgccgccg ccgatcttgg cgcggccatc tggctcgggt tgcccgagcc
     7321 cgaccgcgtc aaggctctgg tcaatcgcgg actggccttt cgcgccgcgg gactgagcaa
     7381 ggaggcagag gccgagattg cgtccgcgcg gaaagtcggc gggagcggcg cggtcgatca
     7441 gcttatcgtc gagggcggag gctcggccga gggcgcggct tcggttgcgg ccttttcgac
     7501 cgaggtccgg caggaaggac aggcgagcgc ttctaacgag cggcgcagct ggttcccgag
     7561 ctttggccgc ggcagcagcg aaacggccgc gcctgcgcct cagccggagc ccgcgccgac
     7621 ccgcaccgcc agcacctcgc cgcaatggtc gaccaccacg agcaaacctg cagacgcggc
     7681 gcccggcgcc tggaacacct cggtcgccgg tgccgagcag ccgagcagca gcgcaagcag
     7741 tggcaaccgt ctcacccgct ggttcggctc gcgcaccgag tcctctcccg ccgccgagcc
     7801 ggagcccgcg cccgcgacgc aaacggccgc cgcgccgccg cagccgagcg cgcccccgtc
     7861 ggcgccgcca gccgcatcga caagctcgag ttggtccacc accaccgaga gcccatccgc
     7921 cgatgcgggc ggaggcgcaa ccaagaggag ctggcgtctc ttcggacgga ccgctgaggc
     7981 tgagcctgcc gcgcaagcgg cggtacagcc ttcggcgtcg ggcggttacc agctccagct
     8041 cgcgaacagc cgctccgagg acgaggcaaa ggcattgtgg aagaaggtct cgcagagcca
     8101 gggtcttgga ggcgtgagtc ccgcaatcga gaaaatcgac atcggcaatt tcggcacgtt
     8161 ctacagcctc aagatcggtc ccttcccgga caaggcgcag agcgttaagg tctgcaacgc
     8221 gttgaagcga agcgggatcg attgctcgcc cgtgacgctt taggcgaaag atccctcaac
     8281 ggcgtagcgg tgttgccgcg gtctgcgtgg gagcttggtc tcacgcgggg accagaggat
     8341 gtgttaaggc actcgggcgt gtagcggcat tctgaaagct tccatgaaga ctgacgacaa
     8401 gccatcgtcc gagaagacag gtgccgcgga ggatccggct caagaccagc tcacccttct
     8461 cggcggcgcg cccgcgcatt cctcgcatct cggcgcgcgc ttgcgcggct atttccttac
     8521 cggcctcatc gtcgtcggac ccatggccat caccctctac gtcgtgtggt ggttcatcaa
     8581 tctggtcgat gcctgggtca agccgctcat ccccaacgcg tacctgcccg aaacctatct
     8641 tcctttcaac gtgcccggtg tcggactcat tgttggaatc ctcggtctga tgctggccgg
     8701 ggctctgacg gcgaatctct tcggccgcac gcttctgagc cacggcgaga tgatgcttga
     8761 ccgcatgccg gtcgtgcgcg gcgtctaccg cctgttcaag cagatcttcc agacgatctt
     8821 ctccaacagc ggaagctcct tcaagcgcgt cgggctgatc gagtttccac gtcgcgggct
     8881 ttattgcatc gtctttctcg cgggcgatcc cccggtggag gtcgatgaca agatcggcgg
     8941 aggcaatccg ctcatcaccg tgttcatgcc caacggtccg aacccgacga ccggcttcat
     9001 cgtgttcgtg ccggcgcgcg aggtgatcct tctcgatctc gcggtcgagg acgcggccaa
     9061 actgatcgtg tcggccggtc tcgtcggccc cgaccagcag caagagcaat tgcgcaggct
     9121 cgccagaacg aaggacacaa aacccgttcc ggaggaagag cccgtcatct aggacatggt
     9181 gcacgtgtaa acacgccggt gcatggcttg ccagagtcca atggagcccc aagccgtctt
     9241 tttttgcgtc cgccatttcc gggatgcgcc attagcggac agtaagtcgc cggcatgggc
     9301 gaagatttag gatttcgttt taggcttgcg catcatcttc ttcgcgattg cctcggcggc
     9361 tattctctct gaaagaggaa tgaacggctg gctactccaa cccggtcgcg aggaagggtt
     9421 actttgcccg ggtttgcata ttgtcacggg atgggcggct atctcttttt ccagtcgagc
     9481 cgcctcctcc tcccgcgtct ctccgattgg cactcgcttg gtcggctttc gcccgcgatt
     9541 aaattgcgtg tttttgttag agctcatcat gacatctctt ggctgttgaa tcgtcagact
     9601 gctggggccg atggttgagt ctcgtgccgg cgctatcgcg accgagcgtt cgagtttctc
     9661 gatggcggaa ttgagcaccc taacgagagc attggccata gaaaaggcgc ctcatgtcac
     9721 tgaggcattg tcgcgaacaa aaactttcag agtgagctcc tttcaatctt caggaataga
     9781 atgatggttc ggtaaagttg gccgaaactc ggcaccgatc caggtagggc ggcgtgcgtg
     9841 ggttccgcat cacagggaac gccatcaaag ggatggccat actcgccgga gggtcaccca
     9901 cgcactgtga aagcgtgcgc tctttacggt tcattgtcga gaattcattt cccgagctca
     9961 ccggcatcac aatggttttt acgatctaaa ttttatcacc gacgctgcgc ttcacttgtt
    10021 cctggacggg ccgaaccgca tcggcggtta cctgattcga taacgagcgc acctcctcag
    10081 cttgagctcc gagcgtgtcg aacttgtagt tccatgatct cagcgaggct gcgcgccccg
    10141 gccaggctct tcgcaagatc gaacccggaa ttgatgttcc gttgggcaat gtcgataacc
    10201 ttacggttga gcgccgcagc gccttgcccg gccgcgccga aggatctctc ccaactctca
    10261 agcaagcctt cgagagcatc cttggagcga tcataaaatt cgcgcgactg agcgagattc
    10321 ttcgcgacca gagctcgcat tgcctctgga gtgtcgcgcg ctatttgctc gaattgaaca
    10381 gcggtcttgg gataagcggc gcctgccttt tcgtcggcga cgtggggttt gttggactcg
    10441 ttcatggcac acctcttggg cttgaatggg gaatgcccga agcccgaagg ctaaggggtt
    10501 gaactcagcg gagaagattt ctcgccgcta gcttagcagc aggttttcga gggcccactc
    10561 gcgaagctca acgggatcgc tcacgccttc agaactgagc atcttcagct tccggctgag
    10621 ggcggccctg agctcgaaat ccctttcaaa atcacgaaac ggatcgcgag cctgtaccac
    10681 cacccacgtc gcttcgaatg cctctgctag ggcgtcagtc gtgcgaatcg aataaggggt
    10741 tcgagggtac attactccct ccatgctttt gcaggcggga gtcttaggct ctcagccgtc
    10801 ggtgcctgtt attgctctta ccggcgatgc cgaagagtgg gcctttccaa gctgaatagc
    10861 gagtcaaaag aatccatcgc tctcaatccg gagcacttag gcgctgcgcg tcacacgcgc
    10921 gagcgcctcg gaggtaacga gaattctgcc atgatcatcg aaaggccctt cagcgtcgaa
    10981 ggcgccactc gcaacatcct caatcagttc gcgataagcc tcaaaaacgg ccccgtcggt
    11041 accggaaaaa tgtacgcggt cggcatgatc tcgcagcgcc tcgtggctca ccctgcacgt
    11101 cacggtgtta ccgtcctcat ccgccatcag aaaccgaacg ccgtcctgtt cgaggaattc
    11161 ctccttgagg cgcgtgaggg gcatcgctcg atcctatcct gctgccagga ctaccaaact
    11221 ttttgatgtg cgcgaagctc ctcctccggc gatgcggtag gaccaccgcc tgt
//
LOCUS       DASUQP010000011         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1095785, whole genome shotgun sequence.
ACCESSION   DASUQP010000011 DASUQP010000000
VERSION     DASUQP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095785"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFY74_00490"
     CDS             <1..557
                     /locus_tag="VFY74_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5930221.1"
                     /translation="IVLDWMLPGISGIELCRRLRASKESRAIPIILLTARSEEGDRIR
                     GLTTGADDYVVKPFSLPELMARVRAILRRTSPERIATVLSVGDIALDRESHRVTRNGR
                     QVHLGPTEFRLLEFLMQSPGRVFSRAQLLDGVWGRNVYVDERTVDVHIGRLRKSMIRG
                     QESDPVRTVRGAGYAFSDTIEETA"
     gene            complement(640..1146)
                     /locus_tag="VFY74_00495"
     CDS             complement(640..1146)
                     /locus_tag="VFY74_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX5930222.1"
                     /translation="MAWTDERVELLKKLWAEGLSASQIAARLGGVTRNAVIGKVHRLG
                     LSGRATSSRSSSPRPRRAHVPRASRASSLLFGTRGNTALKPHYEAEPDLAPAPLEELV
                     IPLNERASILTLKEAMCRWPIGDPGEPEFHFCGRKTCAPLPYCEHHARMAYQPVQARR
                     RERRLASG"
     gene            1534..2721
                     /locus_tag="VFY74_00500"
     CDS             1534..2721
                     /locus_tag="VFY74_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5930223.1"
                     /translation="MPVLVPTYARSELSFARGEGAYLFTASGERYLDFASGVAVTALG
                     HAHPHLVRAVTEQAKKLWHVSNLHRIPEQERLAERLCAATFADMAFFANSGAEAVECG
                     IKAARRYHFKSGAPERYRLVTFDGAFHGRTLATIAAGGQAKHLEGFGPPVGGFDQVAS
                     FDIAAVEAAIGEKTAGILIEPIMGEGGMREVPARFLKELRALADAKGILLLFDEVQTG
                     MGRTGRLFAHEWAGITPDIMATAKGLGGGFPVGACLMTEAVGSAMTAGSHGSTFGGNP
                     LAMAVANAVLDVVLADGFLDHVAEMGVKLKQRLAALTDEHDAIIEDVRGQGLMLGLKC
                     KVPNQKLVEALRAEKMLTVQAGDNVLRFLPPLIVTEVEIDLACTKLDLACVALKSGAL
                     AHA"
     gene            2739..3719
                     /gene="argF"
                     /locus_tag="VFY74_00505"
     CDS             2739..3719
                     /gene="argF"
                     /locus_tag="VFY74_00505"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5930224.1"
                     /translation="MSPKHFLDIDALEAKTLRAIIDMAAAMKRKGRVPKSIKPKPGAV
                     LAMIFEKPSTRTRVSFEVAMHQLGGSSVVLDPQDMQIGRGETLADTARALSRYVDAIM
                     LRTGPHETLEELAAHAGVSVINGLTCRSHPCQVMADVMTFEELKGPIKGRTIAWIGDA
                     NNVATSWVHAAARFGCKLRIGAPKAYAPSPALLAWARGEGAAIEVSEDPLEAARGADC
                     VMTDVWVSMGDNDAKKRAEALRPFRVDGKVMGVAAKDAIFMHCLPAHRGDEVTADVID
                     GPQSAVWEEAENRLHVQKAILAWCLGGDGGKKKRRQAKRAARKIKRSGAR"
     gene            3719..>4337
                     /locus_tag="VFY74_00510"
     CDS             3719..>4337
                     /locus_tag="VFY74_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX5930225.1"
                     /translation="MTEPSSQSQVELRLPEDDLILPFQAEHAEVVGRLVKLGPVVDTI
                     LSRHAYPEPVSQLLGEAVALTALLGAALKFEGKFILQASTDGAVDLLVADYQVPGALR
                     GYARFSAERLAALPAGGRLLGSGHLAMTIDRGPDTERYQGVVPLEGESLAQAADTYFR
                     QSEQLPTFIKLAVARHYRAGHGERPLTWRAGGLLVQKLTRQGGRRA"
BASE COUNT          749 a         1427 c         1414 g          747 t
ORIGIN      
        1 tgatcgtgct cgattggatg ttgccgggta tctccggaat cgagctttgc cggcgcttgc
       61 gcgcaagcaa agagagccgc gcaatcccca tcatcctgct cacggcgcga agcgaggagg
      121 gcgaccgcat caggggactg accacaggcg ccgacgatta cgtcgtcaag ccgttctcgc
      181 tgcccgagct catggcgcgc gtccgcgcca tcctccggcg caccagcccg gagcgcatcg
      241 ccacggtcct gagcgtcggc gatatcgcgc ttgaccgcga gtcgcatcgc gtgacgcgca
      301 acgggcgcca ggtccatctc gggccgaccg agttccgcct gctcgaattc cttatgcaaa
      361 gcccaggtcg cgtgttcagc cgcgcgcaac tgctcgacgg ggtgtggggg cgcaacgtct
      421 atgtcgatga gcgcacggtc gatgtgcata tcgggcggct gcggaaatcg atgatccgcg
      481 ggcaggagag cgacccggtc cgcaccgtgc gcggcgcagg ctatgcgttc tccgatacga
      541 tcgaggaaac cgcctgagcc gcccgcttat cagcagtcat catgaaaaga ggcgccgcgt
      601 gggcgcctct tgcaactctc tgccgcatcg ttcttgcgct caaccgctgg caagccgccg
      661 ctcccgtcgc ctggcctgca ccggctgata ggccatgcgc gcgtgatgct cgcaataggg
      721 aagcggtgcg caggtcttgc gcccgcagaa atgaaactcc ggctctcccg gatcgccgat
      781 cggccagcgg cacatcgctt ccttcagcgt gaggatgctg gcccgctcat tgagcggaat
      841 gaccagctcc tcgagcggcg ccggcgcaag atcgggctcg gcctcgtaat gaggcttgag
      901 cgcggtattg ccgcgcgtcc cgaacaggag cgacgacgcg cggcttgcgc gcggcacatg
      961 cgcgcgacgc ggccgcgggc tcgacgagcg cgagcttgtc gcccggcccg ataagccaag
     1021 tcgatgcacc ttgccgatca cggcattgcg tgtgacgccg cccaagcggg cggcgatctg
     1081 gctcgcgctc aagccctcgg cccagagttt cttcagcaat tcaaccctct cgtcagtcca
     1141 agccatggtt tggcctcctc gaattggact cggaatcccc ccgtctgcct agagacggca
     1201 ttttgaaaac tcggatttac tgctgagatc gaccgcccgg gcgccacgca tgcacccacg
     1261 atatcttgta gccgagcggc aaaactctac tatatccggc agaatccggc aagcgtccgc
     1321 cacgcttgtt cggtccgttt tccaccggga cgcaagcgaa gtctgccact tctttgttcg
     1381 gaacacaaat cgcggcgtgg acggaaaggt ggacgagcat gggataagcg ttgcgcgacc
     1441 gctgattgac aggggtaggc gcgagaccta atgctctgag attcctactg ccgccgatcg
     1501 gcggcaattt tgtttgtgga cagcgacgcc ttcatgccag tcctcgttcc gacttacgcc
     1561 cgcagcgaac tttccttcgc gcgcggcgaa ggggcctatc tcttcaccgc gtccggcgag
     1621 cgctatctcg actttgcgag cggcgttgcc gtgaccgcgc tcggccatgc ccacccgcat
     1681 ctcgtgcgcg ccgtgaccga gcaggcgaag aaattgtggc acgtttccaa tctgcatcgc
     1741 attcccgagc aggagcgcct tgccgagcgc ctctgcgcgg caaccttcgc cgacatggcc
     1801 ttcttcgcca attccggcgc ggaggcggtc gagtgcggca tcaaggccgc ccgccgttat
     1861 cacttcaaat ccggcgcgcc cgagcgctat cggctcgtca ccttcgacgg cgccttccac
     1921 ggaaggacgc tcgccaccat cgccgcgggc ggacaggcga agcatctcga aggttttggc
     1981 ccgccggtcg gtggcttcga ccaggtcgcg agcttcgata tcgctgccgt cgaggcggcg
     2041 atcggcgaga agacggccgg cattctcatc gagccgatca tgggggaggg aggcatgcgc
     2101 gaagtccctg cgcgcttcct gaaagagttg cgcgcgctcg ccgatgcgaa agggattctg
     2161 ctcctgttcg acgaggtgca gaccgggatg ggccgcacgg ggcgcctgtt cgcccacgaa
     2221 tgggcaggaa tcacgcccga tatcatggcg acggccaagg ggcttggcgg cggctttccg
     2281 gtcggcgcct gtctcatgac cgaggcggtc ggttcggcga tgaccgcggg aagtcatggc
     2341 tcgaccttcg gcggcaatcc cctcgccatg gcggtcgcaa acgccgtgct cgatgtcgtg
     2401 ctcgcggacg gctttctcga tcatgtcgcc gagatgggcg tcaagctcaa gcagcggctt
     2461 gcggcgctca ccgatgagca tgacgcgatc atcgaggacg tgcgcgggca aggattgatg
     2521 ctgggactga aatgcaaggt gccgaaccag aagcttgtcg aagcgcttcg cgccgagaag
     2581 atgctgacgg tgcaggccgg cgacaatgtc ctccgctttc tgcccccgct gatcgtaacc
     2641 gaggtcgaga tcgacttggc ctgcaccaaa ctcgatctcg cctgcgtcgc gctcaagagc
     2701 ggcgcgctgg ctcacgcttg agcccaccgg tcaagtccat gtcgccaaag cattttctcg
     2761 atattgacgc attggaggcg aagacactgc gcgccatcat cgacatggct gccgcgatga
     2821 agcgaaaggg ccgcgtgccg aagagcatca agcccaagcc cggcgccgtg cttgccatga
     2881 tcttcgagaa gccttcgacc cgcacccgcg tctccttcga ggtcgccatg caccagctcg
     2941 ggggaagctc ggtcgtgctc gatccgcagg acatgcagat cggccggggc gaaacgctcg
     3001 ccgataccgc acgcgcgctg tcgcgctatg tcgacgccat catgctccgc accggtccgc
     3061 atgaaacgct cgaagagctt gccgcccatg ccggcgtttc ggtgatcaac gggctcacct
     3121 gccggtcgca tccctgccag gtgatggcgg acgtgatgac cttcgaggag ctgaaagggc
     3181 ctatcaaagg ccgcacaatt gcctggatcg gcgatgccaa taatgtcgcg acctcctggg
     3241 tgcacgccgc cgcgcgcttc ggctgcaagc tcaggatcgg ggcgccgaag gcctacgcac
     3301 cctcgccggc actgctcgct tgggcgcgag gcgagggcgc cgcgatcgag gtgagcgagg
     3361 atcctctcga ggcggcgcgc ggagcggatt gcgtgatgac ggatgtctgg gtctcgatgg
     3421 gcgacaacga cgcgaagaag cgggcggagg cgctgcgccc cttccgggtc gacggcaagg
     3481 tgatgggcgt ggccgcgaaa gacgcgatct tcatgcactg cctgcccgcc catcgcggcg
     3541 acgaggtgac cgcggacgtg atcgacggcc cgcaatcggc ggtgtgggag gaggccgaaa
     3601 atcggctgca tgtgcaaaag gcaatcctcg cctggtgcct cggaggcgac ggcggcaaga
     3661 aaaagcgccg gcaagccaaa cgcgccgcgc gcaaaatcaa acgaagcgga gccagatgat
     3721 gacggagccg tcatcacagt cccaagtcga gcttcgcctc cccgaggacg atctcatcct
     3781 gccgttccag gcggagcacg ccgaggtcgt ggggcgtctc gtcaagctcg gccccgtcgt
     3841 cgacaccatc ctctcgcgcc atgcctatcc ggagccggtg tcgcagctgc tcggcgaggc
     3901 tgtggcgctc accgccctgc tcggcgccgc gctcaaattc gagggcaagt tcatcctcca
     3961 ggcctcgacc gacggagccg tcgacttgct ggtcgcggac tatcaggtgc cgggggcctt
     4021 gcgtggctat gcccgcttct cggccgaacg ccttgcggcg cttcctgcag gcggccgttt
     4081 gcttggaagc ggccatctcg ccatgaccat cgaccgtggc ccggacaccg agcgctatca
     4141 aggcgtggtg ccgctcgaag gcgagagctt ggcgcaggcc gccgacactt atttccggca
     4201 gtcggagcag cttccgacct tcatcaagct cgccgtggcg cgccattatc gcgcgggaca
     4261 cggggagcgg cccttgactt ggcgcgcggg cgggcttctc gtgcagaagc tgacccgcca
     4321 gggcggccgc cgggccg
//
LOCUS       DASUQP010000012         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1368581, whole genome shotgun sequence.
ACCESSION   DASUQP010000012 DASUQP010000000
VERSION     DASUQP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368581"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFY74_00515"
     CDS             <1..291
                     /locus_tag="VFY74_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX5930226.1"
                     /translation="KLKRVAGMVPFVEDLVAAYYCALDPATPMRVRGMLLAALAYFIL
                     PFDLVPDIIAGLGFADDAALITAVVGMVAANITPTHRAAAARALDKELPRSS"
     gene            complement(288..1268)
                     /locus_tag="VFY74_00520"
     CDS             complement(288..1268)
                     /locus_tag="VFY74_00520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX5930227.1"
                     /translation="MRVAQISEFGGSEALRLEEVAEPRPGPGEVLIKVTAVGLNFADT
                     LILRNRYQVTPPLPFSPGSEMAGIVEAAGSGVTEIKPGQRVVAYTGGNGCREKVVSKA
                     SNAVAIPDGVSDEAAAALSVTYGTALHGLKDRGRLQPGETVAVLGAAGGAGLAAVEIA
                     KLMGARIIAVASSEEKLAVARAHGAAESIDYAAEDLKTRLKALTKPKGVDVLYDPVGG
                     DYAEAGLRAMAWDGRYLVVGFASGAIPRLPLNLVFLKGCAIVGVYWPAFVEREPERHR
                     ANMVELLDWCRSGLIAPHIHARFALGETAAALALIEGRKVTGKVIVNPQA"
     gene            1399..1953
                     /locus_tag="VFY74_00525"
     CDS             1399..1953
                     /locus_tag="VFY74_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5930228.1"
                     /translation="MKTPLPAARGYFAIGVEGLSKAVNLGNLLRSAHAFGASFVFTIG
                     ADDKAFELRSDTSKASGHLPVYHWRSLKDLKLPKGCSLVGVEILDEAHDLPSFPHPLR
                     AAYVLGPERGALTPELAARCQHLVRIPSAFSLNVATAGAIVMYDRLRALGRFASRPVA
                     EHAEALPPAPHVQGAPRKRRKPVD"
BASE COUNT          391 a          766 c          704 g          412 t
ORIGIN      
        1 aaactcaagc gggtcgcggg gatggtgccg ttcgtcgagg atctggtcgc cgcctattat
       61 tgcgcgctcg acccggcgac gccgatgcgc gtgcgcggca tgctgctggc ggcgctcgcc
      121 tatttcatcc tgccgttcga cctcgttccc gacatcatcg cggggctggg cttcgccgac
      181 gacgcggcgc tcatcacggc ggtggtcggc atggtcgccg caaacatcac gccgacccac
      241 agggcggccg cggcacgggc gctcgacaag gagcttccgc gctcgagcta ggcttgcggg
      301 ttgacgatca ccttcccggt cactttccgg ccctcgatca aggcgagagc cgcagcggtc
      361 tcgccgagcg caaagcgcgc atggatgtga ggggcgatca gcccgcttct gcaccagtcg
      421 agcagctcga ccatgttggc gcggtgacgt tcgggctcgc gctcgacgaa agcgggccaa
      481 tacacgccga cgatggcaca gcccttgaga aacacgagat tgagcggaag gcgcggaatc
      541 gcgcccgagg cgaagccaac aacgagatag cgcccatccc aggccatggc gcgcaggccg
      601 gcttccgcat agtccccgcc gacgggatcg tagaggacat cgacgccttt cggcttggtc
      661 agcgccttga gccgcgtctt tagatcctcg gccgcgtagt caatgctttc agcggcgccg
      721 tgcgcgcgcg caacggcaag cttttcctcc gacgaggcca cggcgatgat gcgtgcgccc
      781 atcagcttgg cgatctcgac ggctgcgagc cccgcgcctc ccgcggcgcc aaggaccgct
      841 acggtctcgc ccggctgaag gcggccgcgg tccttcaacc cgtgcagagc cgtgccgtaa
      901 gtgacggata gggcggctgc ggcctcgtcg ctcaccccgt cgggaattgc gacagcattg
      961 cttgccttgc tgaccacctt ctcgcggcag ccgttaccgc cggtataggc cacgaccctc
     1021 tgaccaggct tgatctcggt gacaccgctg ccggcggcct cgacgatacc cgccatctcc
     1081 gagccaggtg agaacggaag aggcggcgtc acctggtagc ggttgcgcaa gataagcgtg
     1141 tcggcgaagt tgaggccgac cgccgttacc ttgatgagaa cctcgcccgg ccctggccgc
     1201 ggctcggcga cctcctcaag acgcagcgcc tcagatcccc caaactcgga aatttgcgca
     1261 acgcgcatcg cgggctccat catcttcgtg accgagacag atacagcaag gagggcccgg
     1321 ctgatatgcg accgttccgc ggagcgcttg ccgcggtcgc ccttttttga ccggcagggt
     1381 tgtggcagac aggaattcgt gaaaaccccg cttccggctg cccgcggcta tttcgccatc
     1441 ggcgtggagg gcctctccaa ggccgtcaat cttggcaatc tgctgcgctc ggcacatgcg
     1501 ttcggggcga gcttcgtttt caccatcggg gccgacgaca aggccttcga gcttcgttcc
     1561 gacacgtcga aggcctcggg ccatttgcct gtctatcact ggcgaagctt aaaggacctg
     1621 aagctcccca aaggatgcag cctggtcggg gtcgaaatcc ttgacgaggc ccacgatctc
     1681 ccgagctttc cccacccctt gcgcgcggcc tatgtgctcg gtcccgaacg gggcgcgctg
     1741 acccctgaac tcgcggcacg ctgccagcat ctggtgcgga ttccgtcggc attctcgctc
     1801 aatgtcgcaa cggccggggc catcgtcatg tatgaccgtt tgcgcgctct cgggcgtttc
     1861 gcctcgcgcc cggtggccga gcacgccgaa gccctcccgc cggcgccgca tgtacaaggg
     1921 gccccgcgca agcggcgaaa gccggttgac tgatctgcgt agtcgaccaa gaatctcgta
     1981 tcaccttcga atacgcgatg cccaaaatgt caacgattcg atcgccgata ccacggaatg
     2041 atggctgctc accaagatca atctgatccc gttcaatccc tgacccaccg cgcgcgacgg
     2101 ccggcatcgc ctgattaacc gaccagcgct cagcaaatgg tgtcacgcag cggccttctt
     2161 acggtttgcc tcaagccgtg catcgacaag ggcaacgatc gaatcaatgg tggggttgcg
     2221 cagtcccttt tgtgctgcga tggctggctt ggatcagctt gtctgcacgt tcg
//
LOCUS       DASUQP010000013        18008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1913810, whole genome shotgun sequence.
ACCESSION   DASUQP010000013 DASUQP010000000
VERSION     DASUQP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18008
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913810"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(707..952)
                     /locus_tag="VFY74_00530"
                     /pseudo
     CDS             complement(707..952)
                     /locus_tag="VFY74_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005974330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
     gene            complement(1380..2234)
                     /gene="prmC"
                     /locus_tag="VFY74_00535"
     CDS             complement(1380..2234)
                     /gene="prmC"
                     /locus_tag="VFY74_00535"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5930229.1"
                     /translation="MQRAFVEAAQILRQGGIETPELDARLLLCHAASLSHEAFIARAR
                     EDLRPDAAALLEGAIARRLKREPVARIRGTREFYGRSFLLGPESLDPRPDTETLIDAA
                     LDIIGKKGWREEPLKLLDLGTGSGSILVTLLAELPRATGIGTDLSPRALAVAAANASA
                     HGLASRAVFIAADWLDAIAGKFDLILSNPPYLAASEIAGLAADVAAYDPRLALDGGPD
                     GLDSYRRIAARAREVLAGDGRLLVEIGPAQAEAVAAIFAGAGLRPDEAGSPRLDLAGR
                     SRVVVARR"
     gene            complement(2380..3474)
                     /gene="prfA"
                     /locus_tag="VFY74_00540"
     CDS             complement(2380..3474)
                     /gene="prfA"
                     /locus_tag="VFY74_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5930230.1"
                     /translation="MTTIPADKLDKLVARWQTVQGTLAAGADQESYVRLSREFAELDP
                     IVATITALRQAVREREDLKQLVDDPASDPEMTALADEELGALDLKIEELERQLKVQLI
                     PKDAADEKSAILEVRAGTGGEEAALFAGDLFRMYQRYADRHGWKVAVLSASESATGGY
                     KEVIANITGRGVFARLKFESGVHRVQRVPETEAQGRIHTSAATVAVLPEAEEVDIKID
                     DKDLRIDVFRASGPGGQSVNTTDSAVRITHLPSGLVVSQQDEKSQHKNKAKAMKVLRA
                     RLYELERAKRDAERAQDRRSQIGTGDRSERIRTYNFPQGRVTDHRIGFTTYQLPMVLE
                     GAPALDEVIDALITEHQTTQLAAMQEEGHA"
     gene            complement(3545..4546)
                     /locus_tag="VFY74_00545"
     CDS             complement(3545..4546)
                     /locus_tag="VFY74_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930231.1"
                     /translation="MPSGYDAEVAELFRDLRAATALSEMDLASRIGTRPEVVQALEQG
                     ALHALPPWAETCRVVNAYGTMLNLDVRPLLRRIYAQLEAGIIELQPKPMPDVPTMTPR
                     DSDDFGFGSGNVTGTAPSPLDIPWPPPQPPQQPQPQASPQRQWPNAAPAAAPQAGRPN
                     AGPFPQPQSPRPQPQGGQQPSQAWPPSQGWPGGKQPMPQTARPNAPAQRPAPPQAPSD
                     RRPPQPRAPQARAPQPPPAAPRMPAPPEPELLQAEFGPAPTPAPETKRGRSRLLKWGM
                     IALIIAVLAAGLGLLYVYAPGSTGLSTPETSPTDPDDPRSKKTDRLPDAPLPPPPAQ"
     gene            complement(4755..7013)
                     /gene="ptsP"
                     /locus_tag="VFY74_00550"
     CDS             complement(4755..7013)
                     /gene="ptsP"
                     /locus_tag="VFY74_00550"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX5930232.1"
                     /translation="MAIPVGAPRQLLRRLREVMAARESAQARLDRVVVLIASNMVAEV
                     CSLYLRRRDGSLELFATEGLNPMAVHNTHLKPGEGLVGLIAEQAEPMQFPDAQHHPAF
                     SYRPETGEEIYHSFVGVPILRGGNSIGVLTVQNRTMRQYSDEEIEALQTTAMVLAETI
                     ASGGLVTEGLTTEERREGAARFSGVPISEGVALGHVVLHEPRVVVTRLIAEDVEFERR
                     RLAQSIEELTGMIDNMLERGDMARAGEHREVLEAFRMFAHDHGWRRRLDEAMLTGLTA
                     EAAVQRVRNDTRARMLRMPDTHARDRLHDIDDLSNRLLRLLAGGIGTAATGSLPQDAI
                     VIARTMGAAELLDYDRQRLRGLVLEEGGTASHVAIVARALGIAALTQVKGALEAVEPG
                     DAAIVDAEGGELHVRPTPEVVSAYADKVRFRARRQAQYAAFRTVPSVTLDGERISLHI
                     NAGLLFDLPHLDQSGADGIGLFRTELQFMIAATFPRLEQQTRLYKAILDAAGARPVVF
                     RSLDVGGDKVLPYFRGSKEENPAIGWRAIRMALDRPALFRTQVRALLRAAAGRELRLM
                     LPMIADVSEFEAARALIDREVTILKRHGRPEPRKLMVGAMIEVPAVLWQLDRVLPLAD
                     FASVGSNDLMQFLFAADRGNMRVSERFDALNPAGLKPLKLIVDEAARYGVPLNLCGEM
                     AGKPLEAMALIGLGFRSISMAPASVGPVKAMVLSLDAGNLARHLDELLQSKTTSLREE
                     LRRFAVEHGVQV"
     gene            complement(7092..8384)
                     /locus_tag="VFY74_00555"
     CDS             complement(7092..8384)
                     /locus_tag="VFY74_00555"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5930233.1"
                     /translation="MALLVLKFGGTSVADIARIRNVAAHVKREADAGHFCAVVVSAMA
                     GATNQLVTLVEQAWGFKERMQNGGEVSAKYDGREYDAVVSSGEQVTSGLLALVLQSMG
                     LKARSWQGWQIPLNTDAAHRAARITGLAVEELKARMIDGEIAVVAGFQGIAPGNRIST
                     LGRGGSDTSAVALAAALQADRCDIYTDVDGVYTTDPRIVPKAHRLDRISYEEMLEMAS
                     QGAKVLQTRSVELAMQKGVRVLVRSSFDPPDLDGKSTDAALELGTLVCHEDEIVEQNV
                     VSGIAYARDEAKVTLLKVADKPGVAARVFGPLAEANINVDMIVQNVSEDGRFTDLTFT
                     VQSADLDRALEVLRDAKDRIGYLDLRGSTDIAKVSAIGVGMRSHAGVAAQMFSALAEK
                     GINIEAISTSEIKISVLIDAAYAELAVRTLHSLFGLDR"
     gene            8565..9308
                     /gene="ubiG"
                     /locus_tag="VFY74_00560"
     CDS             8565..9308
                     /gene="ubiG"
                     /locus_tag="VFY74_00560"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX5930234.1"
                     /translation="MAETNSTLDRDEVARFAKLAGEWWDANGPFKPLHRINPTRLTYI
                     RDQLCRRFARDAKAGASLKGLSVLDIGCGGGLVAEPLARLGGTVTGIDPAPENIEAAK
                     GHAKGAGLEITYRPVTAEALAQEQGTFDAVLLLEVVEHVPDVPVFLKTVATLVRPGGM
                     MILSTLNRTLKAYALAIVGAELILRWLPAGTHDWNRFVTPDELRGALDRAGLTVSDIT
                     GMVYNPLADEWRLSRDTDVNYFSTALRPA"
     gene            complement(9338..9907)
                     /locus_tag="VFY74_00565"
     CDS             complement(9338..9907)
                     /locus_tag="VFY74_00565"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5930235.1"
                     /translation="MVINIDKASQEMTVFVDGVELYSWPVSTGLRGYSTPSGTYTASS
                     MNKIWYSKQWDNAPMPNAIFFTKKGHAIHGTKEVKHLGKPASHGCVRLSPQNAATLYA
                     LVEANGLEQTQVILTGTTPGGEGPKVAGPGRWPEGYYGYPPWFGPGYGYPPKKKKRGL
                     FGRRWFEPYGPQGYYAPRGDRYYRPRPRY"
     gene            10114..10335
                     /locus_tag="VFY74_00570"
     CDS             10114..10335
                     /locus_tag="VFY74_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930236.1"
                     /translation="MPDPKAKLRRLKAKLPRLEAALRRLRVALPRLEATPPCLKEALP
                     PLKVTAPCLKEALPRLKAMATCLKATLPR"
     gene            complement(10651..11073)
                     /locus_tag="VFY74_00575"
     CDS             complement(10651..11073)
                     /locus_tag="VFY74_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX5930237.1"
                     /translation="MIRFALSCDQGHAFDAWFASGDAFERQVEAHIITCPNCGSRAVR
                     KAPMAPAVNRRRAEAPPPRQVSERKKTYAFLKGLRAHLQANAENVGPAFPEEARKMHY
                     GEAAERSIYGEASLEEARSLQEEGIPAVPLPILPEDHN"
     gene            complement(11070..11948)
                     /locus_tag="VFY74_00580"
     CDS             complement(11070..11948)
                     /locus_tag="VFY74_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5930238.1"
                     /translation="MTLTPNKRFRAALVQLRSGRGVDANVQSAEALIRRAAQSGAAYV
                     QTPENTAIMEFKPELVIESAKTEEESIALARFRTLAAELGIFLHIGSLAIKLDGPRVA
                     NRSYLIDPEGDVAARYDKLHLFDVDLAGGESYRESHHAKPGATAIVADLPFGRLGLSI
                     CYDLRFAALYRALAIAGAEFIAVPAAFTRQTGEAHWHVLIRARAIETGTFVLAAAQGG
                     LHENGRATFGHSLIVSPWGEMLAEGGEEPGVIYADIDLAASAEARARIPALKHGRDFE
                     VALAAPAPRAARREAS"
     gene            complement(11945..12235)
                     /gene="grxC"
                     /locus_tag="VFY74_00585"
     CDS             complement(11945..12235)
                     /gene="grxC"
                     /locus_tag="VFY74_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX5930239.1"
                     /translation="MTRILLYTTPFCGYCRAAKRLLRDKGHEFEEIDVAFDAHKRAEM
                     IDRSGGRRTVPQIFIHGRHVGGHYELAALERAGKLDAWIANSPETLADAEET"
     gene            complement(12293..13081)
                     /locus_tag="VFY74_00590"
     CDS             complement(12293..13081)
                     /locus_tag="VFY74_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX5930240.1"
                     /translation="MSQKPPLRRRRAQRPAPWGSILRRLADFLLPPVCISCRTRIGSH
                     GLLCGACFARIDFIAPPLCDRLGVPLPFEAGEKLLSAAAIASPPVYDRARAAAHYSDT
                     MRELIQSFKYRDRHEGLPLFARWLKKAGAELLADADLILPVPLYATRLWRRRFNQSAM
                     LAVALGRLSALPVDCFVLKRVRRTASQVGLTADQRRRNVSGAFRVDKDKAPQVKGKKL
                     LVVDDVITTGATAEACARVLKRAGAARVDVLALARVVEPTAFVL"
     gene            13166..14104
                     /locus_tag="VFY74_00595"
     CDS             13166..14104
                     /locus_tag="VFY74_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5930241.1"
                     /translation="MNDEHLLFDRKLLRARRARFAHEVAGHAFLLAQVAREITERVGL
                     MLRSFPLALDLGAYHGLLGGSVSQLPSVGETIYAESVLELAALCPRPSLVCDEDLLPF
                     KDASLNLIVSGLALHRVNDLPGALIQIRRALAPDGLFMAAVLGAGALLELRRALLEAE
                     AETQSGASPRVAPFGDVREYGALLQRAGFALPVADAETITVAYATPRDLMREIRALGG
                     GNVLLARTRTPLPRKTLERAESLYHERHGLPDGRVGATFEIVYLSGWGPDPSQQKPLE
                     PGSAARRLADALGTKEQPAGDKAAYPARRRGTVDKG"
     gene            complement(14123..14539)
                     /locus_tag="VFY74_00600"
     CDS             complement(14123..14539)
                     /locus_tag="VFY74_00600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX5930242.1"
                     /translation="MSTANKRPIVIVAACVLLDGEGRVLLAKRPEGRPLAGLWEFPGG
                     KIEVGEEPEDALLRELCEELGIAIAKQDLAPLTFASHAYPDFHLLMPVYLCRRWRGPV
                     TAHEGQELLWVKPDTLHRYDMPPADAPLKIALRDLR"
     gene            complement(14582..15820)
                     /gene="argJ"
                     /locus_tag="VFY74_00605"
     CDS             complement(14582..15820)
                     /gene="argJ"
                     /locus_tag="VFY74_00605"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX5930243.1"
                     /translation="MGKISPFTPEHLPHLPVIDGVRLAATAAGIRYPGRTDLLLALFD
                     PGTTVAGVLTRSKTASAPVDWCRARLPHGMARALVVNSGNANAFTGKRGEEAVTLTAE
                     AAAEAADCLETDVYIASTGVIGERLDGRKIAGALGALAREAKADGFAEAARAIMTTDT
                     YPKLATRKAEIDGVSIDINGIAKGAGMIAPDMATMLAFLFTDAAIEPQALRASLDPAV
                     DETFNAITIDGDTSTSDTALIFATGRSASRGAPRIVNGDDLRLASFRFALHEVLHDLA
                     QQIVKDGEGLSKFVTVKVTGAETKKAARAIAKSIANSPLVKTAIAGEDPNWGRIVAAI
                     GKAGEAADRDSLAIWFGDILVAKDGAVAPSYREKDGAAYMKQPEIAISVDVGVGREAA
                     TIWTCDLTRDYIAINADYRS"
     gene            complement(15839..16747)
                     /locus_tag="VFY74_00610"
     CDS             complement(15839..16747)
                     /locus_tag="VFY74_00610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5930244.1"
                     /translation="MKTRSVLLLTAAIVCLALSPAAAADDPVIARVNDVDIKQSDLDF
                     AASDLGPRLANFSAADRQKVLLQYVMESELIAGAGRTDNVDKAESFPGRLKYYERRAL
                     RDAYFDVKIYDAVTEAEAKKIYDEKIGQVKPEQEIHARHILVETEAEAKDVAERLKKG
                     EDFATLAKEKSKDPGAEGGDLGFFSRGQMVKPFEEAAFALDVGEISAPVQTPFGWHVI
                     KVEEKRDQPLPTFDQVKEGIIAQLVQLKAQEVVTGLRKAAKIEVLDPALKKAMEEEKA
                     MDEAAPKGDAPPLPEDKSSGDAQQKP"
     gene            17016..17456
                     /locus_tag="VFY74_00615"
     CDS             17016..17456
                     /locus_tag="VFY74_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930245.1"
                     /translation="MARRPVARGFALALALFFGCTVPALGDIRILSSGGGSVGQYLNF
                     FAKVRQSGERVIIDGPCLSACTLVLSAVPRKRICVTSRAVLGFHAPYLVDEKGRQYRS
                     REITKTLHAAYPASVRSWIKRKGGLSKKTILLRGRELGALYPRC"
     gene            17589..18008
                     /locus_tag="VFY74_00620"
                     /pseudo
     CDS             17589..18008
                     /locus_tag="VFY74_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086511.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
BASE COUNT         2965 a         5888 c         5995 g         3160 t
ORIGIN      
        1 ggcttcccgc cgcgatcttg cggcgtaagc tcgcaagtcg cagggcggga agggtgaggg
       61 gtcactgact tccgacgctg gccagaattg tcatcctgcg gcagcgcgaa gcgaccctcg
      121 tcaccccggc gaaagccggg gtccaggggc cacaaccgct gtgccggttt gctggattcc
      181 ggctttcgcc ggaatgacag gtatccacgg atgcagcacg aatgcgtcat cctgaggcgg
      241 ccgcgaagcg gccctcgaag gacgcgttcc aatcgtcatc cttcgaggct cgcttcgctc
      301 gcgcctcagg atgacgagcg cgggtctcag gatgacggga atgcctcgag acgcgctgct
      361 caagcggcgc ctaaatcgtg gatggccggg accagcccgg ccatggcgag caaacaggcg
      421 ctagccctta gctgacagaa tcggggacgg tgtcgtcgcc ctgttgtggc tcgggctggt
      481 cggagccgtt ctggtcgccg gcaccttccc ggccgttgcc gccatgacgc cgatcgtcgc
      541 ggccgccgcc ttgacggaac tcgccgccgc cttggcgata ctcgccgccg cgctcgcccg
      601 catcggaatc gccgccctcg tcgtcggagc cttccgcgtt ccagcgctgc tgctggcgat
      661 atccgccatt cggcccctcg ccctgatggc catgctgcgg ccccatctgc gactgcgccg
      721 ccatgatgat gcgattgtag tgctcggcgt gctggagata gctctcggcc gcgaccaagt
      781 cgccggaggc gagggcgtcg cgcgcgagcg acatgtattt ctcggcgata tgcgccgcgg
      841 tgcccctgat cttcacgtcg gggccgttcg attcgaagtt ccgcgccaag ggattttggg
      901 gcttgcggcc accgcggccg cggcccctgc gattctgctg tccttgcctc atgtgctttc
      961 gatcatccaa tctgcgggct gaaccccgcg tctttccctg ctcactctgt cacgtccacc
     1021 ccaggccctc gaagcgcatt gcgcccggaa cgcgggatcg gacgtgcagt ctctcaagct
     1081 gtcgctgctc ttgcctgtct tttgagtcga taccccggcg ccgtgcgtcc aagagtcctt
     1141 tcgccggcgc cagccaacct caatctgaat gaacggttga gcgtctctta aacgtcccga
     1201 gaggcgtcaa gtcctgcaat cgagattcaa gaaaaaccgc tcgtgttgcc tttgcaaccg
     1261 gtccgcgcgc tttcgcaagc gcgaaatggc cctagagtct tccgctgaca cgaacctaac
     1321 ctgaacatcc ccgcctttcc aagtcctttt ttgcccaaag gttcctcgcg cgaccgaaat
     1381 caccgcctcg ccacgaccac gcgggacctg ccggcaaggt cgagcctcgg gcttccggcc
     1441 tcgtcgggcc gaagcccggc gcccgcaaag atcgcggcca cggcctcggc ctgggccggt
     1501 ccgatttcga cgagaagcct gccgtccccg gccagaacct cgcgggcgcg ggcggcgatg
     1561 cggcggtagg agtcaagtcc gtcaggcccg ccgtcgagcg caaggcgcgg atcgtaggcg
     1621 gcgacgtcgg cagcgaggcc ggcgatctcg gacgccgcga ggtagggcgg attggacagg
     1681 atgaggtcga acttgccggc gatcgcgtcg agccagtcgg ccgcgataaa gacggcgcgg
     1741 ctcgccaagc cgtgggcgct cgcattggcg gcggcaacgg cgagagcgcg cggactcaga
     1801 tcggtgccga tccctgtcgc gcgcggaagt tcggcgagaa gggtgacgag gatagagcct
     1861 gagccggtgc cgagatcgag cagcttgagc ggctcctccc gccaaccctt cttaccgatg
     1921 atgtcgagag ccgcatcgat cagcgtctcg gtgtcaggcc gggggtcgag cgattccggt
     1981 ccgaggagga agctgcggcc gtagaactca cgtgttcccc tgatgcgcgc cacaggctcg
     2041 cgcttgagcc ggcgcgcgat ggcgccctca agcagcgcgg cggcatcggg acgaaggtcc
     2101 tcgcgggccc tggcgataaa ggcctcgtgg ctgagagaag cggcatgaca gagcaagagc
     2161 cgcgcgtcga gctctggcgt ctcgataccc ccctgccgca agatctgcgc ggcctcgacg
     2221 aaggctcgct gcatggagcg tctctccccc tctccccagc ggagagaggg ccggggtgag
     2281 ggggacgtgt ccgatgaggt ttgcattccc ctcacccgcg ctccgcgcga cctctccccg
     2341 gtggggagag gtgaaggctt cccggatcgc acaacgggct caggcatggc cttcttcctg
     2401 catggcggcg agctgggtcg tctgatgctc ggtgatcagc gcgtcgatga cctcgtcgag
     2461 cgcgggcgcg ccttcgagca ccatcgggag ctggtaggtg gtaaagccga tgcggtggtc
     2521 ggtgacgcgg ccttgcggga aattataggt gcggatgcgc tcggagcggt cgccggtgcc
     2581 gatctggctg cggcggtcct gggcacgctc ggcgtcgcgc tttgcgcgct cgagctcgta
     2641 aagccgcgcg cgaagcacct tcatcgcctt cgccttgttc ttgtgctggg acttttcgtc
     2701 ctgctggctg acgacgagac ccgagggcag gtgcgtgatc ctgaccgcgc tatcggtcgt
     2761 gttgaccgac tgaccgccgg gaccgctcgc ccggaacacg tcgatgcgca agtccttgtc
     2821 gtcgatcttg atgtcgacct cttccgcctc gggcagcacg gccactgtcg ccgcggaggt
     2881 gtggatgcgc ccttgcgcct cggtctccgg cacgcgctga acgcggtgca cgcccgactc
     2941 gaatttgagc cgggcaaaca cgcctcgacc ggtgatattg gcgatgactt ccttgtagcc
     3001 gccggtcgcc gactcgctcg ccgagagcac ggccaccttc cagccgtgcc ggtcggcgta
     3061 gcgctggtac atgcggaaga gatcgccggc gaagagcgcc gcctcttcgc cgccggtgcc
     3121 ggcccgcact tcgaggatcg cgctcttctc gtcggcggca tccttgggga tgagctgcac
     3181 cttgagctgc cgctcgagtt cctcgatctt gaggtcgagc gcgccaagct cctcgtccgc
     3241 gagcgccgtc atttccgggt cggaggccgg atcgtcgacg agttgtttca ggtcctcgcg
     3301 ctcgcgcacg gcctggcgga gcgcggtgat ggtggcgacg atgggatcga gctcggcgaa
     3361 ctcgcgcgag aggcgcacat agctttcctg atcggcgccc gcggcaagcg tgccttgcac
     3421 ggtttgccaa cgggcgacga gcttgtcgag cttatcggca gggatcgtcg tcatgacatt
     3481 tcacgtcggc aaagggctgg tgagctttcg gtaaccggtc gcccttgccg cctcttaaga
     3541 ccaatcactg ggcggggggc ggcggcaaag gcgcatccgg cagacggtcg gtcttcttgc
     3601 tgcgcggatc gtcgggatcg gtcgggcttg tctcgggcgt ggagagcccc gtcgagcccg
     3661 gcgcatagac atataggagc ccgagtcccg ccgccaaaac cgcgatgatg agggcgatca
     3721 tcccccattt caggagccgg gaccgccctc gcttcgtttc cggcgccggt gttggagccg
     3781 ggccgaattc cgcctggagc agctcgggtt cgggcggcgc gggcatgcgg ggcgccgcgg
     3841 gcggcggttg cggcgcgcga gcctgggggg cgcgcggttg aggcggccgc cggtccgagg
     3901 gggcttgcgg aggcgccggt cgctgggccg gcgcgttcgg ccgggcggtt tgcggcatcg
     3961 gctgtttccc gcccggccag ccttgcgaag gcggccaggc ttgcgacggc tgttgtccgc
     4021 cttgcggctg cggcctcggg ctttgtggct gcgggaacgg gcctgcgttc gggcgtccgg
     4081 cctgaggcgc cgcggccggc gccgcatttg gccactgcct ctgtggggag gcttgcggct
     4141 ggggctgctg gggtggctgt gggggcggcc agggaatatc gaggggactg ggcgccgtgc
     4201 ccgtcacgtt gccggaaccg aagccgaaat cgtcggaatc gcgcggcgtc atcgtgggga
     4261 catcgggcat gggcttcggc tgaagctcga tgatccccgc ttcgagctga gcatagatcc
     4321 gccgcaaaag cgggcggacg tcgagattga gcatcgtgcc gtaggcgttg acgaccctgc
     4381 acgtctccgc ccatgggggg agcgcatgga gcgcgccctg ctcgagcgcc tgcaccactt
     4441 ccggccgggt cccaatgcgc gacgcgaggt ccatttctga gagcgcggtt gccgccctca
     4501 gatctcgaaa cagctccgcc acctcagcat catagccgga gggcatattc ttgttcccgt
     4561 gaatgggctg gcgccgcggt gcggcgtccg gctgtgagtc taccggtact cgacgcggcg
     4621 cggtcggcat cgcaaggccg atcccttctc aagcttgtct ccgccaacac aagcccaacc
     4681 gcatccggtt tgcacgtcgg ggggagacta gcacatcggg gcctcgggtt aagacttctc
     4741 atcaggctaa aacgtcagac ctgcacaccg tgttcaacgg caaagcgccg taattcctcc
     4801 cgcagcgatg tcgtcttgct ctgaagcagc tcatccaggt gccgggcaag attgccggca
     4861 tcgagcgaaa gaaccatggc cttgaccggt ccgaccgagg cgggcgccat cgagatcgag
     4921 cggaagccga ggccgatgag cgccatggcc tcgagcggct tgcccgccat ttcgccgcag
     4981 aggttcagcg gcacgccgta ccgcgccgcc tcgtcgacga tcaacttgag cggcttgagt
     5041 ccggcggggt tcagcgcatc gaaacgctcg gagacgcgca tattgccgcg atcggccgcg
     5101 aacaggaatt gcatcaggtc gttcgagccg acggaggcga aatcggccag gggcaggacg
     5161 cggtcgagct gccaaagaac cgccggcacc tcgatcatcg cgccgaccat gagcttcctg
     5221 ggctcggggc gcccatggcg cttgaggatg gtgacttcgc ggtcgatcag cgcgcgggcc
     5281 gcctcgaact ccgagacgtc ggcgatcatc ggcagcatga ggcgaagctc gcggcctgcc
     5341 gccgcccgga gcagcgccct cacttgcgtg cggaacaggg ccggccggtc gagcgccatg
     5401 cggatcgcgc gccagccgat ggcggggttt tcttccttgc tcccgcggaa gtagggcaac
     5461 accttgtcgc cgccgacatc gagcgaacga aacacgacgg gccgcgcgcc ggcagcgtcg
     5521 aggatggctt tatacagccg ggtctgctgc tcgaggcgcg gaaaggtcgc cgcgatcatg
     5581 aactggagct cggtgcggaa caggccgatg ccgtcggcgc ccgactgatc gagatgcggc
     5641 aagtcgaaca ggagcccggc attgatgtgc aaggaaatcc gctcgccgtc caaggtgacc
     5701 gagggcacgg tgcggaaggc ggcatattgc gcttggcgcc gggcccggaa gcgaactttg
     5761 tcggcatagg ccgagaccac ttccggggtc ggccggacat gcaactcgcc gccttcggcg
     5821 tcgacgatcg cggcgtcgcc cggctcgacc gcttcgagcg cgcccttgac ctgggtcagc
     5881 gcggcaatgc cgagcgcgcg cgccacgatg gcgacatgac ttgcggtgcc gccctcctcc
     5941 agcacgagcc cgcgcaggcg ctggcggtcg tagtcgagca gctcggcggc ccccatggtg
     6001 cgcgcgatca cgatcgcgtc ctgaggcagg ctccccgtcg ccgccgtccc gattccgccc
     6061 gcgagcagcc gcagcagtcg gttcgacaga tcgtcgatat cgtgcagccg atcgcgcgca
     6121 tgggtgtcgg gcatgcgcag catgcgcgca cgcgtgtcgt tgcgcacgcg ctgcaccgcg
     6181 gcctcggccg tgagacccgt gagcatcgcc tcatcgaggc gccgccgcca gccatgatcg
     6241 tgagcgaaca tacggaaggc ttccaacacc tcgcgatgct cgccggcgcg cgccatatcg
     6301 ccgcgctcca acatattgtc gatcatgccg gtcaactctt cgatcgattg ggcgagccgg
     6361 cgacgctcga actcgacatc ctcggcgatg aggcgcgtga ccacgacgcg cggctcatgc
     6421 agcaccacat ggccgagcgc cacgccttcg gagataggaa cgccgctgaa acgcgcagca
     6481 ccctcacgcc gctcctccgt cgtcagcccc tcggtcacaa ggccgcccga ggcgatggtt
     6541 tccgcgagca ccatggccgt ggtttgcagg gcctcgatct cctcgtcgct gtactgccgc
     6601 atggtgcggt tttgcaccgt gagcacgccg atggagttgc cgccgcgcag gatcggcacg
     6661 ccgacgaagg agtggtagat ctcctccccc gtctcgggac ggtaggagaa ggccggatga
     6721 tgctgggcat cggggaactg catgggctcg gcctgctccg cgatcaggcc gaccaggcct
     6781 tcgcccggct tgagatgggt attgtggacg gccatcgggt tgaggccctc ggtcgcgaac
     6841 agctcgagcg aaccgtcgcg gcggcgcaga tagagcgagc acacctcggc caccatgttc
     6901 gaggcgatca gcaccaccac tctgtcgagg cgcgcttgcg cgctctcgcg cgcggccatg
     6961 acctcgcgca ggcggcgcag caactgtctc ggggcgccaa caggaatagc cataaccaac
     7021 agtcctcgcc ggacggcaaa gcggcagatc ccgcagtgca agaaagcttc aggccggccg
     7081 ccaaaaatcc gctagcggtc caaaccgaac aaggaatgca aggttcgcac cgcaagctcg
     7141 gcgtaggccg cgtcaatcaa cacgctgatt ttgatctcgg atgtggagat cgcctcgata
     7201 ttgatgcctt tttcggcaag cgcgctgaac atctgcgcgg caaccccggc gtggctccgc
     7261 atgccgacac ctatcgccga taccttggcg atgtcggttg agccgcgcaa gtccaaataa
     7321 ccgatcctgt ccttggcgtc gcggaggacc tcaagagccc gatcgaggtc ggcgctctgc
     7381 accgtaaagg tgagatcggt gaagcggccg tcctcggaga cgttctgcac gatcatgtcg
     7441 acattgatat tggcctcggc gagcggaccg aatacgcgcg cggcgacgcc tggcttgtcg
     7501 gcgaccttga gcagggtcac cttggcctca tcgcgcgcat aggcaattcc gctgacgacg
     7561 ttctgctcca cgatctcatc ctcatggcaa acgagtgttc caagctcaag cgccgcgtcg
     7621 gtgctctttc cgtcgaggtc gggcggatcg aagctcgacc gcaccagcac cctgaccccc
     7681 ttctgcatgg caagctcgac cgagcgggtc tggagcacct tggccccttg cgaggccatt
     7741 tctagcattt cttcgtagga gatgcggtcg agccggtgcg cctttggcac gatcctgggg
     7801 tcggtcgtgt agacgccgtc gacgtcggta taaatgtcgc agcgatcggc ctggagcgcc
     7861 gcggcaagcg cgaccgcgct cgtgtcagag ccgccgcggc cgagcgtcga gatgcggttc
     7921 cccggcgcaa tcccctggaa gccggcaacg acggcgatct cgccgtcaat catgcgcgcc
     7981 ttcagctctt ccacggcaag gccggtaatg cgcgctgcac gatgggcggc gtcggtgttg
     8041 agcgggatct gccagccctg ccaggagcgc gccttcagcc ccatcgattg cagcacgagc
     8101 gcgagcagcc ccgaggtgac ctgctcgcca gaagacacca cggcgtcgta ttcgcgaccg
     8161 tcatatttgg ccgagacctc gccgccattt tgcatccgct ccttgaagcc ccaggcctgc
     8221 tcgaccaggg tcacgagctg gttggtagcg ccggccatgg ccgacaccac cacggcgcaa
     8281 aaatggccgg catcggcctc gcgcttcaca tgcgcggcga cgttccggat ccgggcgatg
     8341 tcggcgacgg aggttccgcc gaacttcaac accaagagcg ccatggcctc gttccttgtg
     8401 ggcggtagcc cacaataaga gggccggccc tttaagggac cggccggaca tgcgtgcctt
     8461 tcatactgat tggcggcgca tgccgcaacc acgctctcct ggcctgagag aggttaagga
     8521 tgcgtggctt gacaggaacg gggccggagg gcctttcctc tgcaatggcc gagacgaaca
     8581 gcaccctcga ccgggacgag gtcgcccgct tcgccaagct cgcaggcgaa tggtgggatg
     8641 cgaatggccc gttcaagcct ttgcaccgca tcaacccgac gagactaacc tatatccgcg
     8701 accagctttg ccggcggttc gcgcgtgacg caaaggcagg cgcaagcctc aagggcctgt
     8761 ctgtcctcga catcggctgc ggcggcgggc ttgttgccga gcccctggcc cggctcggcg
     8821 gcacagtcac cggaatcgac ccggcgcccg agaatatcga agcggccaag ggccacgcga
     8881 aaggcgccgg acttgaaatc acctaccgcc ccgtgacggc ggaggccttg gcgcaggagc
     8941 aaggcacctt cgatgccgtg ctcctgctcg aagtggtcga gcacgtgccg gacgttcccg
     9001 tctttctcaa aacggtcgcg accttggtga ggccgggcgg catgatgatc ctctcgacac
     9061 tgaaccgcac gctcaaggct tacgcgcttg cgatcgtcgg cgcagagttg atcttgcgct
     9121 ggctgccggc cggaacacat gactggaacc gcttcgtcac gcccgatgag ctccgcggcg
     9181 cgctcgaccg cgccgggctc accgtctctg acatcacggg gatggtctac aacccgctcg
     9241 ccgacgagtg gcggctatcg cgggacaccg acgtgaacta tttctccacg gcgctgcggc
     9301 cagcctagac attcgcctac tgggggcgcc ttgttgacta gtagcgcggg cggggccggt
     9361 agtaccggtc gccgcgcggc gcgtagtagc cctgcggacc gtacggctcg aaccatcgcc
     9421 gcccgaacag gccgcgcttc ttctttttcg ggggatagcc atagccgggc ccgaaccagg
     9481 gcgggtatcc gtaatagccc tcaggccacc tgccgggccc ggccactttc ggcccctcgc
     9541 cgcccggtgt ggtgccggtc aagatgacct gggtctgctc caacccgttc gcctcgacga
     9601 gagcgtagag cgtggcggca ttctgcggag acaaccgcac acagccatgc gatgcgggtt
     9661 tgccgagatg cttgacctcc ttcgtgccat gaatggcatg gcccttcttg gtgaagaaga
     9721 tcgcattagg catgggcgcg ttgtcccact gcttgctgta ccagatcttg ttcatcgaag
     9781 aggcggtata ggttcccgac ggggtggaat agccgcgcaa gccagtcgag acaggccagg
     9841 aataaagctc aacgccgtcg acgaagacgg tcatctcttg cgatgctttg tcgatattga
     9901 tgacgatgct ggatttcggc tgcgcgggct cggcactcgg cgcactctcc gccgctccgg
     9961 aattttcgga tgctccgttc gtctgcccgg acggcgcggc aggtttaggc ccaccgtcct
    10021 gggccgtccc ggaggtctga ggtgctgacg ggcttgcaga tggcgcgctc tgaccggagg
    10081 ttgccggcgg cgtgctggga ctggtcgcag aggttgccgg acccgaaggc gaagctgcgc
    10141 cggctgaagg cgaagctgcc gcgtttggag gcggcgctgc gccgtttgag ggtggcgctg
    10201 ccccgtttgg aggcgacgcc gccgtgcttg aaggaggcgc tgcccccgtt gaaggtgacg
    10261 gcgccgtgct tgaaggaggc gctgccccgg ttgaaggcga tggcgacgtg cttgaaggcg
    10321 acgctgcccc ggtaggagcg gtctcaggcg ttgcaggact cgaccgggtt gcggccggcg
    10381 gcgcattgga gctcgtctcg gcacccgcgg aaaacgcgag catcgtcgcc aaaaacggta
    10441 ccgcgaggag gctccgaact cgtggaaagg aagcagagaa ccgcatctgt tagaccctgg
    10501 ttgcgcgaaa ccctaacgcc cggcgacttc atacatatga catcttagcg gccataacag
    10561 ggcgccgcgc ggtattttgt aagggtaagg accgaataat aagttacgcg cgatgataac
    10621 gccggtttgt aatcctgtct ttcgcctccg tcagttgtga tcctcgggca agatcgggag
    10681 cggaacggcg gggatgccct cttcctgcaa cgacctcgct tcctcgagac tagcctcgcc
    10741 ataaatgctg cgttctgccg cctccccgta atgcatcttg cgcgcctcct cggggaaggc
    10801 ggggccgaca ttctcggcgt tcgcctgaag atgagcgcga aggcccttga ggaaggcata
    10861 ggtcttcttg cgttcgctga cctgccgggg cgggggcgcc tcggcgcgtc gccggttgac
    10921 ggcaggcgcc atcggcgcct tccgcaccgc ccggctgccg caattggggc aggtgatgat
    10981 gtgcgcctcg acctggcgct cgaaggcgtc gccagaggcg aaccaggcgt cgaaggcgtg
    11041 gccttgatcg caactgaggg cgaagcgaat catgaggcct ctctcctcgc cgcgcgcggc
    11101 gcgggcgccg cgagcgcgac ttcgaagtcg cggccatgct ttagggccgg gatgcgggcg
    11161 cgcgcctcgg cagaggcggc aaggtcgata tcggcataga tcacgccggg ctcctcgccg
    11221 ccttccgcca gcatctcgcc ccagggcgag acgatcaggc tatggccgaa ggtggcgcgg
    11281 ccgttttcgt gcagaccccc ctgggcggcg gcgagcacaa aagtgccggt ctcgatggca
    11341 cgggcgcgga tcagcacgtg ccaatgggcc tcacccgttt gtcgcgtgaa ggcggcgggt
    11401 acggcaatga actcggcccc ggcaatagca agcgcccggt agagagcggc gaagcggagg
    11461 tcgtagcaga tcgaaagccc caaccggccg aacggaaggt cggcgacgat tgcggtcgca
    11521 ccgggcttgg cgtggtgcga ctcgcggtag ctctcgcctc cggcaaggtc gacgtcgaag
    11581 agatgcagct tgtcgtaacg cgcggccaca tccccctccg gatcgatcag ataggagcga
    11641 ttggcgaccc ggggtccatc gagcttgatg gcaagcgaac cgatgtggag gaaaatgcca
    11701 agctcggccg caagcgtgcg aaaccgcgcg agcgcgatac tctcctcctc ggtcttcgcc
    11761 gattcgatca caagttcggg cttgaattcc atgatcgcgg tgttttccgg cgtctgtaca
    11821 taagcggcgc cgctttgggc ggcgcggcgg atcagcgcct ctgcgctctg cacattggca
    11881 tcaacgccgc ggccggaccg aagttgcacc agcgcggcgc ggaaccgctt gttcggtgtg
    11941 agcgtcatgt ctcttcggcg tcagcaagcg tctcggggct gttcgcgatc caggcatcga
    12001 gcttgcccgc gcgctcgagc gcggcaagct cgtaatggcc tccgacatgc cggccatgaa
    12061 tgaagatttg cggcacggtg cgccggcctc ccgagcggtc gatcatctcg gcgcgcttgt
    12121 gcgcatcgaa ggcgacgtcg atttcctcaa actcgtgtcc cttgtcgcgc aggagccgct
    12181 ttgcggcgcg gcagtaaccg cagaaggggg tggtgtagag taggatgcgc gtcattggcc
    12241 gagaattcta tggttccctg ataactgggc cgcaaccggc ccccgcgcaa gactagagca
    12301 cgaaggcggt gggctcgacg acgcgcgcca gcgccaaaac atcgacccgg gccgctcccg
    12361 cgcgcttcaa cacccgggcg caggcctcgg ccgtcgctcc cgtcgtgatc acatcgtcga
    12421 cgaccaagag cttcttgccc ttgacctgag gcgccttgtc cttgtcgacc ctgaaggccc
    12481 cggagacgtt gcgccgccgc tgatcggcgg tgagacccac ctggcttgcc gtccgccgta
    12541 cccgcttcag cacgaagcag tcgacgggta gcgcacttaa acgtcccaag gccacggcga
    12601 gcatggccga ttggttaaac cgcctccgcc acagccgcgt ggcatagagc ggcaccggaa
    12661 ggatgagatc ggcgtcggcg agcagttctg cgcctgcctt cttgagccaa cgcgcaaaga
    12721 gtggcaggcc ctcatgccgg tcccgatatt tgaagctctg gataagctcg cgcatggtgt
    12781 ccgaataatg cgcagcggcc cgcgcgcggt cgtaaactgg aggcgaggcg attgccgccg
    12841 ccgagagcag cttctcgccg gcctcgaacg gaagcggcac gccgagccgg tcgcaaagcg
    12901 gaggcgcgat gaaatcgatc ctggcgaagc aggccccgca taagagcccg tggctgccga
    12961 tgcgggtgcg gcaagagatg cagacaggcg gcaaaagaaa gtcggcaagg cgccggagga
    13021 tcgaccccca tggcgccgga cgctgcgccc ggcgtcgccg gagcggcggt ttctggctca
    13081 aggccgagct ctgcctcacc tgccggcatg gcggccatga taggacctga agggccgcgc
    13141 acaaagcctt gaacacacgc gggcgatgaa cgacgaacac ctcctcttcg atcgcaaatt
    13201 gctgcgggcg cggcgcgcgc gtttcgcaca cgaggtcgcc ggccacgcgt ttcttctcgc
    13261 ccaagtcgcg cgcgagatca cagaacgcgt cgggctcatg ctccggtcgt ttccgcttgc
    13321 gctcgatctc ggcgcctatc acggattgct cgggggatcc gtgtcgcaac ttccctcggt
    13381 cggagagacg atctacgcgg agagcgtgtt ggaactcgcg gcgctctgtc cccggccttc
    13441 gctcgtttgc gatgaggatt tgctgccctt caaagacgca agcctcaatc tcatcgtctc
    13501 ggggctcgcg ttgcatcgcg tcaacgatct tcctggggcg ctgatccaga tcaggcgcgc
    13561 gctcgctccc gacggattgt tcatggcggc ggtgctcggc gcgggcgcgc ttctggagct
    13621 ccgccgcgct ctcctcgaag ccgaggccga gacacagagc ggtgcaagcc cgcgggtcgc
    13681 acccttcggc gatgtgaggg agtatggagc gcttctccag cgcgcgggct ttgcccttcc
    13741 cgtcgccgac gccgagacca tcacggtcgc ttacgcgact ccgcgcgacc tgatgcgcga
    13801 gatccgcgcg ctcggcggcg gcaacgtgct gcttgcgcga acgcgaacgc cccttccgcg
    13861 caagacgctt gagcgcgccg agagccttta tcacgaacgc catggcctgc ccgatggaag
    13921 ggtcggcgcg acgttcgaga tcgtctatct cagcgggtgg ggacccgatc cgagccagca
    13981 gaaaccgctc gaaccgggaa gcgccgcgcg gcgtcttgcc gacgcgctcg gcaccaaaga
    14041 gcagccggcg ggagacaagg ctgcctatcc ggcgcgccgc cgcgggactg tcgacaaggg
    14101 ttgacggcga aaggttcgtc ggtcagcgga gatcgcgcag cgcgatcttg agcggcgcgt
    14161 cggccggcgg catgtcatag cggtgaagcg tatcgggctt cacccaaagg agctcctggc
    14221 cttcgtgcgc cgtgaccggt ccccgccagc gccggcagag ataaaccggc atcagcaaat
    14281 ggaagtcagg ataggcatgg ctcgcgaatg tgagcggcgc aaggtcctgc tttgcgatcg
    14341 cgatgccgag ctcctcgcaa agctcgcgca acaacgcgtc ctcgggctcc tctcccacct
    14401 cgatcttccc gccgggaaac tcccatagcc ccgcaagcgg ccgcccttcg ggccgcttgg
    14461 cgagcagcac cctgccctcg ccgtcaagca gaacgcaggc cgctacgatg acgatcgggc
    14521 gcttgttagc cgtgctcatc gtgcatccga agacagacgg cacattggcg tgagatcaag
    14581 ctcacgaccg ataatcggcg ttgatggcga tgtagtcgcg ggtgaggtcg caggtccata
    14641 ttgtcgccgc ttcccggccg acgcccacat cgacgctgat ggcaatctcc ggctgcttca
    14701 tataggccgc gccgtccttc tcgcgatagg agggcgccac cgcgccgtcc ttggcgacga
    14761 ggatgtcgcc gaaccagatg gcgagactgt cgcggtcggc cgcctccccc gctttgccta
    14821 ttgccgcgac gatccgaccc caattggggt cttcgccggc gatcgccgtc ttcaccagcg
    14881 gcgaattggc gatcgatttt gcgatcgcgc gcgccgcctt cttcgtctcg gcgcccgtga
    14941 ccttcaccgt gacgaacttg ctgagcccct cgccgtcctt gacaatctgc tgggcaagat
    15001 cgtgcaacac ctcgtgcagc gcgaagcgga agctcgcgag cctgagatcg tcgccgttga
    15061 cgatgcgcgg agcgccgcgc gaggcggacc gccccgtggc gaagatgagc gccgtgtcac
    15121 tcgtcgacgt gtcgccgtcg atggtgatgg cgttgaaggt ttcatcgacg gcagggtcga
    15181 gcgaggcgcg cagcgcctgc ggttcgatgg cggcgtcggt gaaaagaaag gcgagcatgg
    15241 tcgccatgtc gggcgcgatc atgccggcgc ccttggcgat gccgttgatg tcgatcgaga
    15301 ctccgtcaat ctcggccttg cgcgtggcaa gcttggggta ggtatcggtg gtcatgatgg
    15361 cgcgcgccgc ctcggcaaag ccgtcagcct tggcttcgcg cgcaagcgcg ccgagcgccc
    15421 ccgcgatctt gcgcccgtcg agccgctccc cgatcacgcc ggtcgaggcg atatacacat
    15481 cggtctcaag acaatcggcg gcttccgcgg ccgcttcggc ggtcagcgtc accgcctcct
    15541 ctccgcgctt gccggtgaac gcgttggcgt tgccggaatt gaccacgagc gcgcgcgcca
    15601 tgccgtgggg aagtcgggcg cggcaccaat cgacgggcgc ggacgccgtc ttcgaccgcg
    15661 tgagcacccc cgccacggtc gttccgggat cgaacagagc aagcaagagg tcggtgcggc
    15721 cgggatagcg tatcccggct gcggttgccg cgagccgcac gccatcgatc accgggagat
    15781 gcggcaggtg ctcaggcgtg aagggagaga tcttgcccaa ggggtctcta tcaagcggtc
    15841 acggcttctg ttgcgcatcc ccggaggatt tgtcctccgg gagaggcggc gcgtcgccct
    15901 tcggcgcagc ctcatccatc gccttctcct cctccatcgc cttcttcagc gcgggatcga
    15961 gcacctcgat ctttgccgcc ttgcgcaggc cggtcaccac ctcttgcgcc ttgagctgca
    16021 cgagctgggc gatgatcccc tccttgacct gatcgaaggt cggcagcggc tggtcgcgct
    16081 tctcctcgac cttgatgaca tgccagccga acggggtctg gaccggggct gagatttcgc
    16141 caacgtccag cgcgaaggca gcctcttcga acggcttgac catctgcccg cgcgaaaaga
    16201 acccgaggtc gccgccctcg gcgccgggat ccttggactt ttccttcgcg agcgtggcga
    16261 agtcttcgcc cttcttgagc cgctcggcga cgtccttggc ctcggcctcg gtctccacca
    16321 ggatgtggcg ggcgtgtatc tcctgctccg gcttcacctg gccgatcttc tcatcataga
    16381 tcttcttggc ttcggcctcc gtcaccgcgt catagatctt gacgtcgaaa taggcatcgc
    16441 gcagcgcccg gcgctcgtag tatttcagcc gtcccgggaa gctctcggcc ttatcgacgt
    16501 tgtcggtccg gccggcgccc gcgatcagct cgctttccat cacgtattgg agcagcacct
    16561 tctggcgatc cgcggccgag aaattagcaa gccgcggccc gagatcggag gccgcgaaat
    16621 cgaggtcaga ctgcttgata tcgacgtcat tgacgcgggc gataacagga tcgtcggccg
    16681 cggcggcagg agagagggca aggcagacga tggctgccgt aagcagcaag actgaccgcg
    16741 ttttcacgag aatggtcctc ttcttcggct cgcctcaggc tcgctccgga tgtcgagcag
    16801 gcgaacgggt tgggtttgtt tccccagggc tcgcgtttta gacccgaggc ggcgggttgc
    16861 caaggggcga ggggccggcc gggcaggttc acttgcggcg acccatcaat cgacctgttc
    16921 agaccgtttt gaggccataa acttggtgga ccatagccga cttccgctgg aggcgaggaa
    16981 tgaatgccgc acgttttggt tgctcccgat cggtcttggc gcgacgcccg gtcgcgaggg
    17041 gttttgcgct cgcgctggct ctcttcttcg gatgtacggt gccggcgctc ggcgacatcc
    17101 gcatcctgtc aagcggcggg ggctccgtcg gacagtatct gaacttcttc gccaaggtca
    17161 ggcaatcggg cgagcgtgtc atcatcgacg gtccttgcct ctcggcctgc acgctcgtgt
    17221 tgagcgccgt tccgcgcaag cggatctgcg tcacgtcgcg ggcggtcctc ggctttcatg
    17281 cgccctactt ggtggatgag aaagggcggc agtaccgctc gcgcgagatc accaagaccc
    17341 tgcatgccgc ctaccccgcc agcgtcaggt cgtggatcaa gcggaagggc ggactcagta
    17401 aaaaaaccat actgctcagg ggccgcgaac tcggcgcact ctatccgcgc tgctaacggc
    17461 ggtgccgttg gcggacaggc gcgccgtgta aagcgtcatg atcgaacgcg acatataaaa
    17521 gtatctctcg tgtttggagt gttcgcggtg tcatccaact gtcatcggcg tcgcaatagc
    17581 gtgagaccat gaccgcgaac cgcccgctga acgtgctctt cctctgcacc ggcaattcgg
    17641 cgcgctcgat cctggccgag gccatcctga accgattggg cgccggcaag tttcggggct
    17701 atagcgccgg aagccacccc accggcaagg tcaatccttt ggcgctgaat cttctgcgca
    17761 aggcgaatta cgacgtctcc cggctccgct cgaaatcgtg ggaggagttt gcagagccaa
    17821 gcgcgccgaa gctcgacttc gtattcaccg tgtgcgacga cgcggccaac gaagtctgcc
    17881 ccatctggcc ggccaaccga tgtccgctca ctggggcctg cccgatcccg tcaaagcgca
    17941 gggcacagag gccgagcgca acctcgcctt cgcagacacg atgcgcatgc tgacccagcg
    18001 catcgata
//
LOCUS       DASUQP010000014         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_688244,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000014 DASUQP010000000
VERSION     DASUQP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688244"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1878)
                     /locus_tag="VFY74_00625"
     CDS             complement(49..1878)
                     /locus_tag="VFY74_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5930246.1"
                     /translation="MPKRHSYILGINAYDHDVSACLLRDGAIAFAIAKERINRHKHAT
                     GFYQEVVDYCLRAEGIALDDVDLVVRNCYVLPVGEMEKRLTYQDVPEVLPPKDRKQAA
                     ASPLYRSRSNKVVDVSHHLAHAYSAFAVCPFSEGALMVVDGVGNYASDVEEAHRIDNL
                     NPLARESESYYRFEGSKLEPLKKVWLEPTIGFLADEFFNMNGLGAVYSRVSSYIFADW
                     NKCGEVMGLAPYGSPDRIKPLVEFRNGALNVPEWTEDFNQPYLPDDDAKWENDPGMQH
                     WKDLAWRVQDDAERVLIERAIWLRETTGAKNLCISGGVALNCVANGKIVREAGYDNVW
                     IQPAAGDDGIAIGCAYYGHLAIQNKPRSFVMEHAYLGAPYRDEEAEAAAKRWLVRSRT
                     CKSPEENICAETAKVLAEEKVVGWFQGGSEFGPRALGNRSILADPRKAEMKDLLNTRV
                     KHRQAFRPFAPIVLAERAREVFEGDEDSPFMLLAKRVRPEWKDKVAAIVHVDGTARVQ
                     TVRREHNERLYLLLEEFEKITGVPVLLNTSFNIRGEPIVETPADAMKCFLSTGIDYLA
                     MHDLLIAKSPYYPFFYPIVKWGSDVATMVRVGIGADLGRDGEA"
     gene            2067..4316
                     /gene="parC"
                     /locus_tag="VFY74_00630"
     CDS             2067..4316
                     /gene="parC"
                     /locus_tag="VFY74_00630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX5930247.1"
                     /translation="MGRRIAPPSDSGPIEPVDLREALEERYLAYALSTIMHRALPDVR
                     DGLKPVHRRLLYAMQQLRLSPQGEFRKCAKIVGDTMGNFHPHGNQAIYDALARLAQDF
                     SVRYPLIDGQGNFGNIDGDNPAADRYTEARLTAVAAHLLDGIEEDTVDFRANYDGREM
                     EPSVLPATFPNLLANGASGIAVGMATNIPPHNAGELCDAALHLIKFPNATADKLVDFV
                     PGPDLPTGGVIVEPRAEIVQAYRCGRGSFRLRARWTQEDTGRGTYQIVVTEIPYQVPK
                     ARLVARIAELLLQKKLPLLADVRDESAETVRLVLEPKSRGVDPVLLMEQLFRLTELET
                     RLPLNMNVLSRGQVPNVLGLRQVLREWLDHRQIVLVRRSKFRLAKIDHRLEVLDGYLI
                     VYLNLDKVIKIIREKDEPKPALMKAFKLSDVQAEAILNMRLRALRKLEEMEIRREHDA
                     LAKERKELKALLASKDKQWARIAGEIKALKAELGQKTALGKRHTSFAEAPEGVETELA
                     EAMIEKEPVTIVLSEKGWIRAIRGHQAETDSLAFKEGDRLKRAVKAQTTDTMLLFATN
                     GRFYALGADKLPGGRGHGEPFRLMIDIEEAADAVELFVHEPGRKLLVAGTSGHGFVVA
                     EDEVLAMTRKGKQVLNVPSGEEATVCAPVDGDSVAVIGDNRKLLLFTLAELPEMPRGK
                     GVRLQRYKDGGLADAKVFSKKEGLTYIDAAGRSFVLTELRDWWGARAQAGRLPPKGFP
                     KSNSFGPKF"
     gene            complement(4347..5129)
                     /locus_tag="VFY74_00635"
     CDS             complement(4347..5129)
                     /locus_tag="VFY74_00635"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX5930248.1"
                     /translation="MTEHKNNFPEFYITAPQPCPYLPGRLERKLFTHLTRDKPRFLID
                     NLLKGGFRRSQNIAYMPYCDACSACVPVRILVEEFAPRRSLKRIARINSDLVATRMQP
                     APSAEQYALFRDYIDVRHADGGMAEMSVLDYSLMVEDSVVETFVTEYRVKPDPHGDGS
                     SPLVAVSLCDRLSDGISMVYSFYDPSQPQRSLGTYMILDHVAYARAQARPFVYLGYWI
                     EGARKMRYKNRFMPQERLTSKGWVRSDAPESVARSHKLQTVR"
BASE COUNT         1008 a         1730 c         1717 g          942 t
ORIGIN      
        1 gcacaacatg gagatttgcg aaacgctgcc gattcgccgg tagttccctc acgcctcgcc
       61 atctcgccca agatcggccc caatcccgac acggaccatc gtggcgacgt ccgagcccca
      121 tttcacgatc ggataaaaaa atgggtaata ggggctcttg gcgatcagca aatcgtgcat
      181 ggccagatag tcgatgcctg tggacaggaa gcatttcatc gcatcggcgg gcgtctcgac
      241 gatcggctcg cctctgatgt tgaacgaggt gttcagcaat acggggacgc cggtgatttt
      301 ctcgaactct tcaagaagga gatagagacg ctcattgtgc tcccgccgca cggtctgcac
      361 ccgtgcggtg ccgtcgacat gaacgatcgc tgcgaccttg tccttccact cagggcgcac
      421 gcgcttggca aggagcatga acggggagtc ttcgtcgccc tcgaacacct cacgcgcccg
      481 ctcggcaagg acgatcggcg cgaatggcct gaacgcctgg cgatgtttga cccgcgtatt
      541 cagcaaatcc ttcatctcgg ccttgcgcgg gtcggccagg atgctgcgat tgccgagcgc
      601 gcgcggcccg aactccgaac cgccttgaaa ccagccgacc actttctcct cggccagcac
      661 cttggccgtc tcggcgcaga tattctcctc gggtgacttg caggtcctgc tgcgaacaag
      721 ccatctcttc gccgccgcct cggcctcctc atcacgatag ggcgcgccga gataggcgtg
      781 ttccatgacg aagctgcgcg gcttgttctg gattgccaga tgcccgtaat aggcgcagcc
      841 gatggcgatg ccgtcatcgc cggcggccgg ctgaatccac acattgtcgt agccggcctc
      901 ccgcacgatc ttgccgttgg cgacgcaatt cagcgccacg ccgccggaga tgcagagatt
      961 tttggcgccc gtcgtctcgc gcagccaaat ggcgcgctcg atcaggaccc gctcggcatc
     1021 gtcctggacc cgccacgcga ggtccttcca atgctgcatg ccgggatcgt tctcccattt
     1081 cgcgtcgtca tcgggcagat agggctggtt gaagtcttcg gtccactcgg ggacgttcaa
     1141 cgcgccgttc cggaattcga ccagcggctt gatccggtcc gggcttccat agggcgcaag
     1201 acccatcact tcgccgcatt tgttccaatc ggcgaagatg tagctcgaca cccggctata
     1261 gaccgcgccg agaccgttca tgttgaaaaa ctcatcggca agaaagccga tcgtcggttc
     1321 gagccacacc ttcttcagcg gttcgagctt tgacccttcg aagcggtagt agctctcaga
     1381 ctcccgggcg agcggattca gattgtcgat ccggtgggct tcctcgacat cgctggcgta
     1441 gttgccgacc ccgtccacca ccatcaacgc gccttcggag aacggacaga cggcaaaggc
     1501 gctgtaagcg tgcgcgaggt gatgggaaac gtcgacgacc ttgttggagc gcgaacggta
     1561 gagcggactc gcggccgcct gtttccggtc ctttggcgga agcacttcag gcacgtcctg
     1621 gtaggtcagg cgcttctcca tttcgccgac cggcagcacg tagcaattgc gaaccacgag
     1681 atcgacgtcg tcgagggcga tgccctcggc gcgcaggcag taatcgacga cttcctggta
     1741 gaacccggtc gcgtgcttgt gccggttgat ccgctccttg gcgatggcga aggcgatcgc
     1801 gccgtcgcgc aagagacagg cgctgacgtc atggtcgtag gcgttgatgc cgaggatata
     1861 gctgtgacgc ttgggcacgg ttccactctc aggaaggtcg cataacgcga aggatggctg
     1921 cgaggtccct gacgatgggg gcagggcggg gaagaaacga tcactgctta tagccgatat
     1981 ggaggctgcc gcaatgcgcc gggaagcgcg cgcccgtcgg gaagcgaggc cttgagcgcg
     2041 acccgccgat gcgctaagcg cgacccatgg gacggcgcat cgcaccgcca tctgattcgg
     2101 ggccgatcga gccggtcgat ttgcgcgagg cgctggagga gcgctacctc gcctacgcgc
     2161 tctccaccat catgcaccgc gcgctgcccg acgttcgcga cgggctcaag cccgtgcacc
     2221 ggcgcctgct ctacgccatg cagcagctca ggctcagccc tcagggcgag ttcaggaaat
     2281 gcgcgaagat cgtcggcgat accatgggca atttccatcc ccacggcaat caggcgatct
     2341 acgacgctct ggcgcggctc gcgcaagact tctccgtccg ctatccgctg atcgacgggc
     2401 agggcaattt cggcaatatc gacggcgata accccgcggc cgaccgctac accgaggcgc
     2461 ggctgacggc ggttgccgcg catctgcttg acggcatcga ggaggacacg gtcgatttcc
     2521 gcgccaatta cgacgggcgc gaaatggaac cctcggtcct tccggccacg ttccccaacc
     2581 ttctcgccaa tggcgcctcc ggcatcgccg tcggcatggc gaccaacatc ccgccgcata
     2641 atgccggcga gctttgcgac gcagcactcc acttgatcaa gttcccgaac gcgacggcgg
     2701 acaagctggt cgatttcgtg cccggccccg atctcccgac cggcggggtg atcgtcgagc
     2761 cgcgcgccga gatcgtccaa gcctacagat gcggtcgcgg cagcttccgg ctccgcgccc
     2821 gctggaccca agaagatacc ggccgcggca cctatcagat cgtggtcacc gagattccct
     2881 atcaggtccc gaaggcgcgg ctcgtcgccc gcatcgccga gcttctcctg cagaagaagc
     2941 tgccgctgct tgccgacgtg cgggacgaat cggccgagac cgtgcgcctc gttctcgagc
     3001 cgaagagccg cggcgtcgat ccggtcttgc tcatggagca gctgttcagg ctgacggagc
     3061 tcgagacgcg gcttccgctc aacatgaacg tgctgagccg cgggcaagtg cccaacgtgc
     3121 tcggtttgcg ccaagtcttg cgcgagtggc tcgaccaccg ccaaatcgtg ctcgtccgcc
     3181 gctcgaaatt ccgcctcgcc aagatcgatc accggctcga agtgctcgac ggctatctca
     3241 tcgtctatct caacctcgac aaggtcatca aaatcatccg cgagaaagac gagccgaagc
     3301 ccgctctgat gaaggcgttc aagctctcgg acgtgcaggc cgaagccatc ctcaacatgc
     3361 ggctccgcgc tctgcgcaag ctcgaggaga tggagatcag gcgcgagcac gatgccctcg
     3421 ccaaggagcg caaggaactg aaagcgctgc tggcctctaa ggacaagcag tgggcgcgga
     3481 tcgccggcga gatcaaggcg ctcaaagccg agcttggcca gaagacggcg ctcggcaaac
     3541 gccacaccag cttcgccgag gcccccgagg gcgtcgagac cgaacttgcc gaggccatga
     3601 tcgagaagga gcccgtcacc atcgtcctat ccgagaaagg ctggatccgt gccatcaggg
     3661 gccaccaggc ggagaccgac agcctcgcct tcaaggaggg cgaccggctg aagcgcgcgg
     3721 tcaaggcgca gacgaccgac acgatgctcc tcttcgccac caatggccgc ttctacgcgc
     3781 tcggcgccga taagctgcca ggcggcaggg gccacggcga gccgttccgc ctgatgatcg
     3841 acatagagga ggcggccgac gccgtcgagc tgttcgtgca cgaaccgggc cgcaagcttc
     3901 ttgtggccgg aacgagcggc catggtttcg tcgtcgccga ggatgaagtc ctcgccatga
     3961 cgcggaaagg caagcaggtg ctgaacgtgc cctcggggga agaagcgacc gtctgcgcgc
     4021 cggtcgacgg cgacagcgtg gcggtgatcg gcgacaaccg gaagctcctg ctgtttacgc
     4081 tcgccgagct gcctgagatg ccgcgcggca agggcgtgcg gcttcagcgc tacaaggacg
     4141 gggggctcgc cgacgccaag gtgttcagca agaaagaggg cctgacctat atcgacgcgg
     4201 cgggccgctc tttcgtcctc accgagttgc gggattggtg gggagcgcgc gcccaggccg
     4261 ggcggcttcc gcccaagggc ttcccgaaat caaattcgtt cggacctaag ttctgaccgt
     4321 catgggtcgt attgagcagg aaaagctcag cgcaccgtct gcagcttgtg gcttctggct
     4381 accgattccg gcgcgtcgct ccgcacccag cccttcgagg tcaggcgttc ctgcggcatg
     4441 aagcggttct tgtagcgcat cttgcgcgcc ccctcgatcc aatagccgag atagacgaac
     4501 gggcgggctt gcgcgcgggc gtaagcgaca tgatcgagga tcatgtaggt cccgaggctg
     4561 cgctgaggct ggctcggatc gtagaagctg tacaccatcg agatgccgtc gctgagccgg
     4621 tcgcagagtg aaacggcgac gagaggcgac gagccgtcgc catgcggatc gggcttgacc
     4681 cgatactcgg tgacgaaggt ctcgaccacg ctgtcctcga ccatgagcga atagtcgagc
     4741 accgacatct cggccatgcc gccgtcggcg tggcggacgt cgatatagtc gcggaacaac
     4801 gcatattgct ccgcgctcgg ggccggctgc atgcgtgtcg cgacgagatc ggaattgatg
     4861 cgggcgatgc gcttgagcga gcgcctgggc gcgaactcct cgaccaggat gcggacgggc
     4921 acgcaagcgg agcaggcgtc gcaatagggc atataggcga tgttctggct ccgccggaac
     4981 ccgcccttca ggaggttgtc gatgaggaaa cgcggcttgt cgcgggtgag atgcgtgaac
     5041 agcttccgct cgagccgccc cggcagatag gggcagggct gcggcgccgt gatgtagaat
     5101 tcggggaagt tgttcttgtg ctccgtcacg tccgatcgac cgcaggcgaa tccaccgatc
     5161 cggcaagctt agtggatcga tggccggaag cgcaatcggc ctcgctcggt taaaaccgcc
     5221 ggcgcctcct gtcagaattg ggcgcaagct tcatccctcc cttgatggga gggtggcgcg
     5281 gccgaaggcc gagccgggtg atggcctttt tagccgcgac cacttggccg tggcatgccc
     5341 cccaccccga cgccgccttg cgctcaggca gcggagcggt agcgcaaacg taacggc
//
LOCUS       DASUQP010000015        19905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1370554, whole genome shotgun sequence.
ACCESSION   DASUQP010000015 DASUQP010000000
VERSION     DASUQP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 19905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19905
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370554"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFY74_00640"
     CDS             <1..265
                     /locus_tag="VFY74_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930249.1"
                     /translation="PERIERLARKHLKLGPARPTQIVTLDTVSDRDFDRSRLEPEASS
                     AVPAAKSKPIEKPKVSIASAPAVDTAAPWPQAKIVRPVEASAQ"
     gene            278..1954
                     /locus_tag="VFY74_00645"
     CDS             278..1954
                     /locus_tag="VFY74_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5930250.1"
                     /translation="MTDSARPDPAASRAERAYLARGRYRFRLACLAFALAFTLIAARL
                     VTLGFAATGPNAGGLFDISSTVHRPDIFDRNKRLLATDIKGATLFADPARVIDVDELV
                     EHVASVLSNIDQRDLRTRLRQGRRFVRIKRELTPKQQAEIHELGVPGLGFVEEYRRVY
                     PMGATAGHVVGLVDVDSKGLAGIEKFIDDNPQLAMIGAEGVTLSLDLGAQHVLREELG
                     RAIGTYRAKAAAGIVLDVHSGEVVALVSLPDYDPNRREQALDKERLNRIGFGVYEMGS
                     VFKVFTVAGVLDGGQATLRSHYDASSPIHYASFTIDDFHGKRRSLTVPEVFIYSSNIG
                     AAKMALDMGVERHRNFLKKLGLMSRVTTELGDSASPIVPQHWQKLNTMTIAFGHGLSV
                     TPLQLAAASQPLVNGGIAVTPTFLPRSREEGLSGGKRVLKRETSAAMLKLWRLNVLKG
                     TGKHADAEGYRVGGKTGTAEKVVGGRYSTSALLTSFLAAFPTDAPEYLVLVMLDEPQR
                     VAESNNQATAGVNAAPTAGKIIERIAPILGVAPKLERDQRKFDAKVLASY"
     gene            2031..3491
                     /locus_tag="VFY74_00650"
     CDS             2031..3491
                     /locus_tag="VFY74_00650"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5930251.1"
                     /translation="MTLGELFGRDAKLPAGLERVPIEGLAADSRDVRPGYLFAALPGV
                     KTDGARFIADALQRGAAAILVPHGAKPPTGSAAIIEDSDPRRRLALIAARFFALQPQT
                     TVAVTGTNGKTSVASFVRQLWSGEGFKAASLGTVGVVSPSGTKTLIHTTPDPIELHRI
                     LAALATEGVTHLALEASSHGLQQRRVDGIVFAAGAFTNISRDHLDYHASFEDYFAQKL
                     RLFTELLPRGATAVVDIDSDAGKHIAAVAEAKGLDLVTVGREGKTLRLVSAARDGFGQ
                     RLIVEHGGKQHQVRLPLVGAFQASNALVAAGLVMATGTSAATVLPMLEALQGARGRLD
                     LAGTARAGAPIFIDYAHTPDALAKALDALRPYVERRLVVVFGCGGDRDTGKRPEMGRV
                     AVTKADLAIVTDDNPRSEIPAAIRSQILQAAPGALEIGDRARAITEAIGTLERGDVLL
                     IAGKGHETGQTIGTKVIPFSDHDAVAAALNQEVKCG"
     gene            3484..4902
                     /locus_tag="VFY74_00655"
     CDS             3484..4902
                     /locus_tag="VFY74_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HEX5930252.1"
                     /translation="MAEPLWTLGEIVHATRGACLRAEGAPVFGFSIDSRSLAPGEAFI
                     AIRGPNRDGHAFVATALDKGATCAIVDKTFPPGDEQKLVRVGDTLEALNDLGRGARTR
                     ASRTKVIAVTGSAGKTGTKEALRLALAPSGSVHASAKSFNNHWGVPLSLANMPRTVRF
                     GVFEIGMNHAGEIDALTRLARPHIAIVTTVAPVHLGFFPSVEAIADAKAEIFSGLEPG
                     GTAIINRDNPYYERLKHHALNHGATIVGFGEARDAEARLRDVTLDPDGSNVSADILGE
                     TIAYRLGAAGRHVVQNSLAVLAAAKVAGADLALAAGALAGLEAQAGRGARNVIETEGG
                     RMAIIDESYNANPASMRAALATLGLTPRSAFRRRIAVLGDMLELGPEGFRLHRELAEF
                     IDGVGVDVVFACGEQMGSLFEALPASRRGAYAKTAEELGPKLLEAVGPGDAIMVKGSL
                     GSRMAPLVEALKRRFGKEAAPA"
     gene            4931..6016
                     /gene="mraY"
                     /locus_tag="VFY74_00660"
     CDS             4931..6016
                     /gene="mraY"
                     /locus_tag="VFY74_00660"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5930253.1"
                     /translation="MLYWLAGFAPELPIFNVFRYITFRTGGALLTALLFVFLFGPKVI
                     RSLRIRQGKGQPIRKEGPERHLIQKQGTPTMGGLMILSGVTVAVLLWGNLQNTFVWVV
                     LLLTLVYGAVGLYDDYLKVTKQSAQGFRGRIKIVIEGAAALVATTVIVYASQEPLATS
                     LTIPFIKDALINLGWLFIPFGAFIIVGAGNAVNLTDGLDGLAIVPVMIAAATFGFISY
                     LVGNALFADYLQIHFVPGTGELAVICGALVGAGLGFLWFNAPPALIFMGDTGSLALGG
                     ALGTIAVATKHELVLAIVGGLFVLEAVSVIVQVTSFRLFGKRVFRMAPLHHHYEQKGW
                     PESTVVIRFWIIAVVLALAGLATLKLR"
     gene            6028..7446
                     /gene="murD"
                     /locus_tag="VFY74_00665"
     CDS             6028..7446
                     /gene="murD"
                     /locus_tag="VFY74_00665"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5930254.1"
                     /translation="MIPVTIFAGRDVAILGLGLSGLASARALEAGGATPILWDDKASA
                     REEAAGQGFTVSDLSAADWSGFAALVLAPGIPLTHPAPHWSVARARNAGVEVIGDVEL
                     FFRERARARTPGKVVAITGTNGKSTTTALVAHLLAAAGKAVALGGNIGKAVLDLDPFA
                     PGLTYVLELSSFQIELSPSLSPDAAALLNITPDHLDRHATLAHYARIKASVFAGLGAR
                     ATAVVGVDDEVSRAIADGLDGPYAVKRIAVGRDVETGVFVTDGILHEMEGGGEKARID
                     LAGIGSLRGAHNWQNAAAAYALARSQGLSPAAIAEGLKSFAGLSHRMEQVARRGKVLF
                     VNDSKATNADAAGKALASFTDIYWIIGGRPKEGGLAGLEPFYPRIVRAYLIGEAAEAF
                     ASQLGGHVDHVHCGTLARAVEAAASDASRSAAKEPIVLLAPACASYDQFDNFAKRGDA
                     FRDLVMGLDGAEGVRPEQGQAA"
     gene            7443..8618
                     /gene="ftsW"
                     /locus_tag="VFY74_00670"
     CDS             7443..8618
                     /gene="ftsW"
                     /locus_tag="VFY74_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX5930255.1"
                     /translation="MRLTRADRSVLSDWWFTVDRLMFFGLLLLMGAGLVLSLAASPPI
                     AAKYDLEPFYFVRRHAALIVPAVAIMFAASTLTPKQIRRASLVIFLVGIALMALIFGF
                     GPEIKGAKRWLQIGIVSIQPSEFVKPAFVVLTAWLFNESQKRRDVPGIELGVALYAIF
                     AALLVLQPDFGQTLLVTLVWGALFYMAGINMVWIGALAVAGIAGIVVAYFLIPHVTSR
                     IDRFIDPASGDTYQADRSLDSFLHGGWFGRGPGEGTVKNVLPDSHTDFIFAVTAEEYG
                     LIACLILLVLFAFIVLRGLSKAAQEPDGFIRHAVAGLIMLFGLQALINMAVNVGLLPA
                     KGMTLPFISYGGSSLLAMALTMGFALGLTRKRPMAGRLPAADAHRDAESERIREQAA"
     gene            8615..9721
                     /gene="murG"
                     /locus_tag="VFY74_00675"
     CDS             8615..9721
                     /gene="murG"
                     /locus_tag="VFY74_00675"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX5930256.1"
                     /translation="MTARPFLLAAGGTGGHLFPAAALAQELKRRGYDVELATDIRAEK
                     YGVEFPARAIHRIPSATLTSRNPFAVTVTFTRLGVGFFGALRLLMTSKPRAVIGFGGY
                     PTLPPILAARASGIPTAIHEQNAVMGRANRLLSRFVDRVALSFKPTRLLRTDAEAKAR
                     VTGTPVRDAVLSYREAPYEPPEPGGPLLLLVFGGSQGARFFSEALPPALARLPKEMRE
                     RLTVVQQAREEDIDELQEGYKKAGIAAHIAPFFRDLPERIAKSHLVVARAGASTVAEL
                     MAIGRPCLLVPLPHALDNDQLENATRLQEGGGGWCVRQQELTPERLAGELQRLLSSPE
                     VLAATAARGKAMAETEAVSKLADLAEELARSKAE"
     gene            9736..11166
                     /gene="murC"
                     /locus_tag="VFY74_00680"
     CDS             9736..11166
                     /gene="murC"
                     /locus_tag="VFY74_00680"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX5930257.1"
                     /translation="MHLPDPDRAGLFHVVGIGGIGMSAIAEVMHTRGYRVQGSDLKDG
                     ANLDRLRGRGVTCLIGHDPANVTGAGHLVISSAVKPGNPEFEAAKERGIPIIRRAEML
                     AELMRPCATVSITGTHGKTTTTSLIADVLKCADLDPTVIAGGIINGWGTNARIGRGEW
                     MVVEADESDGTFLKLPTQIGVVTNIDPEHMDYWPSIEALHQSFLQFIEAIPFYGVAVA
                     CIDHPVVRELLAKLGGAANGRRTLTYGVARDADIRLGNLRASRDRIVFDAELGHAVRG
                     GARKLEDLMLPVPGHYNALNALGALAAATEAGVSDDVIRKAFAAFSGVKRRFSRVGDF
                     DGVSVYDDYAHHPVEIAAVLRAARIGGDGRIVAVVQPHRYTRLQSLFNEFSACLDDAD
                     VVVVTPVYSAGEAPIPGIDRDELASGLRRHGHPNVLTIDDEETLVETIASVAKPGDIV
                     VGLGAGTITDWMNALPKKLEQHRGRA"
     gene            11163..12083
                     /gene="murB"
                     /locus_tag="VFY74_00685"
     CDS             11163..12083
                     /gene="murB"
                     /locus_tag="VFY74_00685"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX5930258.1"
                     /translation="MNGRDLESRLTASLPELRGRLKAEAPLKDLTWFRAGGPAEVLYS
                     PADEADLAYFLKATPADIPVTVIGLGSNLLVRDGGIAGVVIRLGRGFGEIAIEDGTRL
                     RAGTAVPDVKVARAAAEAGIAGLSFYRGIPGCVGGALRMNGGAHGRETKEVLIEVRAV
                     DREGNTHVLPVAELGYAYRSCGAPEDFIFTEALFQGEPGDPAKILAEMDDIAAYREEV
                     QPIKSRTGGSTFKNPPGHKAWQLIDRAGCRGLSVGDAKVSEMHCNFLINEGNATGADL
                     ETLGETVRARVKETSGVELEWEIKRLGIPA"
     gene            12201..13136
                     /locus_tag="VFY74_00690"
     CDS             12201..13136
                     /locus_tag="VFY74_00690"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5930259.1"
                     /translation="MANKQVHVVVLMGGLSAEREVSLRSGKACADALEVQGYRVSRLD
                     VGQDVAARLAELAPDVCFNALHGRYGEDGCIQGVLETMGIPYTHSGVLASALAMHKER
                     AKAVLAAAGVPIAESLVIARTEAARAHAMPPPYVIKPPAEGSSVGVFIVREDHAHPPQ
                     QLTASEWTFGEEVMIERFIPGRELTCAVMGEEPLGVIEIRPAEGLAFYDYDAKYAPGG
                     SIHVLPAPLKPNVYQLVQKLSLAAHRALGCRGVSRSDFRYDDRPEGTGEVICLEVNTQ
                     PGMTSTSLVPELAAHSGRSFGELVRWILEDASCNR"
     gene            13142..14110
                     /locus_tag="VFY74_00695"
     CDS             13142..14110
                     /locus_tag="VFY74_00695"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX5930260.1"
                     /translation="MARRRGSREPLLARGIAAPRKVSSRLPVLAEARRLITRSPRTIR
                     LGRIGGHARSKWLVLCALFLGSALVYGAVIGGQTGRAYDALTNGINRLAIAAGFGVKK
                     ITVAGQLHVTDATITAALGAGPDTMMLGFDTDAAKERLEAVPWIRHAQVMRLLPSTLQ
                     VVVEERTPFAVWQKNGQTFVVDPEGVVLAPALRDAYAALPLVVGEGAGKNAAQLVEQL
                     AAHDDLKQHLLAAIRVGDRRWTLKLISGTDIMLPDDNVPEALASLAKLDGEREVLKRD
                     IAAVDLRLLDRITVRLRAAEEAKPQGGTPQADMPTASTNAAAKGKT"
     gene            14136..15431
                     /gene="ftsA"
                     /locus_tag="VFY74_00700"
     CDS             14136..15431
                     /gene="ftsA"
                     /locus_tag="VFY74_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5930261.1"
                     /translation="MGYTTSIRGKRQRIVTALDIGTSKICCLIAKAQPSPDWLDGRGD
                     AMQFEVLGFDHTRAEGLKAGMIAHLDSAETCIRSAVDAAERMAGVTVEDVQVSVTCGR
                     LKSETFSASVALASGTVREDDIRRLLGGGRQYAGRDKRNVLHALPLSYRLDDNAGISE
                     PQGMCGERLAVDLHAVTADEVAMRNVMLCVERCHLGVASLIAAPYASALAVVTPDEAK
                     LGVACIDFGAGTTTLSVLADGQFIHTDAIAFGSNSITTDIARTLAAPLDYAERLKTLH
                     GAAFATLSDEREIITYPAVAGMSQASLNQITKAQLVAIIRPRVEETLDLLRRRLAASG
                     IAAEVTQHLVLTGGGSQLTGLPELTANMFGRPVRLGRPRSLRGLPSAAASPDFAVGVG
                     LLLNWAKGDDRQSGAAEQRFLRTGTGYFAKVGEWIRDNF"
     gene            15514..17190
                     /gene="ftsZ"
                     /locus_tag="VFY74_00705"
     CDS             15514..17190
                     /gene="ftsZ"
                     /locus_tag="VFY74_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5930262.1"
                     /translation="MTINLGVPDLTELKPRITVFGIGGAGGNAVNNMIAAGLKGVEFV
                     VANTDAQALASSTAQRRIQMGTHVTEGLGAGSKPEIGAAAAEEAMADIKAHLVGCHMV
                     FITAGMGGGTGTGAAPIIARASREEGILTVGVVTKPFHFEGARRMRASEVGIEQLQKF
                     VDTLLIIPNQNLFRVANEKTTFTDAFAMADDVLRAGVSCITDLMVKEGLINLDFADVR
                     TVMAGMGKAMMGTGEASGDRRAIDAAEAAISNPLLDDVCMRGASGLLVSITGGNDLTL
                     YEVDEAASRVRQEADAEANIIVGATFDDELDGSIRVSVVATGIGGAQAEMPPANPQER
                     NRLSKRLASLATLPGMPVDHDEPSLVLAEAEMVKEAPVWRGPGNVTIEKRPAQVTAIG
                     PVGVSQPKQAPQSASRPFEPAPPVGIKRAVRRMPTMDELPVVTQNEIKAKSGETPVIG
                     LAAQKKKVGFLERLANVGRSRKEPDEPPAKREPEFERPWGELPKMAERAKPEPEAAAP
                     RGIRIERPRGEKVVAALPKLVEVQPALETPDESPADDDLEIPAFLRRRAN"
     gene            17499..18449
                     /gene="lpxC"
                     /locus_tag="VFY74_00710"
     CDS             17499..18449
                     /gene="lpxC"
                     /locus_tag="VFY74_00710"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX5930263.1"
                     /translation="MRGFIGARQTTLASEITLNGTGVHSGAPVSITLCPADGDTGIRF
                     LLSNGNGHETEIAADQRSVAGVTLCTVLGDGNGASVATVEHLLAALRGLGVDNALIEI
                     DSAEVPIMDGSAARFVEAIDEVGLAELETPRRYLKVLKKVVVEDAGAYGELAPHNGFH
                     LDVEIDFDTPLIGNQRIEIDLNPGSFRRELARARTFGFMKDVERLWAAGLALGASLDN
                     TVAIGDDRVINREGLRFSDEFVRHKALDAVGDLALAGAPILGAFRSHRGGHRLNALVL
                     KALFADAEAWTMVEAPRYREIRHAELSHRLAAANFAAERS"
     gene            18684..19595
                     /locus_tag="VFY74_00715"
     CDS             18684..19595
                     /locus_tag="VFY74_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5930264.1"
                     /translation="MSGRLERERAATPPSGPSRAALALPARLACVLVAANLLSACGSF
                     GSFGWFGNKDDEETVSNDPPNVIYGKAEALVDRGKYKDAARAYEEVDINHPYSPEARK
                     AILMAAYAYYKGGKYDDAIGAADRYLTLHPGTQESDLAQNIIAMSYYDRVLDPRRDQT
                     NARKALTAYDTLLQRYPESRYAADARNRGRILRDLLAANEMMVGRYYLRHNNFLAAIN
                     RFRTVVTDFQTTEQIEEALMRLTEAYMALGIVNEAQTAAAVLGHNFPESKWYRHAYGL
                     LGKRGLKPQEHEGSWITQTWRGDGRQT"
     gene            19606..>19905
                     /locus_tag="VFY74_00720"
     CDS             19606..>19905
                     /locus_tag="VFY74_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5930265.1"
                     /translation="MLEALAIRDIVLIDKLDLEFGEGLSVLTGETGAGKSILLDAFSL
                     ALGGRGDAALVRRGANQGQVTAIFALDRSHPVSALLAANGIEPETALILRRVQGAD"
BASE COUNT         3397 a         6675 c         6421 g         3412 t
ORIGIN      
        1 cccagaacgg atcgagcggc tggcgcgcaa gcatctgaag ctcggaccgg ccaggcccac
       61 ccagatcgtc accctcgaca cggtctctga ccgcgatttc gaccggagcc gcttggagcc
      121 ggaggcctct tcggcggtac cggcggcgaa atccaagccg atagagaagc caaaggtctc
      181 aatcgccagt gcaccggccg tcgatacggc tgcgccctgg ccacaggcca agatcgtccg
      241 gccggtcgaa gcctcggccc aataggcggt aaccaccgtg accgacagcg caaggccgga
      301 tcccgccgct tcccgcgccg agcgcgccta tctcgcgcgc ggccgttatc gcttccgtct
      361 cgcctgtctt gccttcgccc ttgctttcac cctcattgcg gcgcgcctcg tcacgctcgg
      421 cttcgccgcc accggaccga acgccggcgg gctgttcgac atctcctcga cggtccaccg
      481 tcccgacatt ttcgatcgca acaagagatt gcttgcgacc gatatcaagg gcgcgactct
      541 tttcgccgat cccgcgcgcg tgatcgacgt ggacgagctg gtcgagcatg tcgcgagcgt
      601 cctttccaat atcgaccaac gagacctccg gacgcggttg aggcaggggc ggcgcttcgt
      661 tcgcatcaag cgcgagctga cgccgaagca gcaggccgag atccacgagc tcggcgtccc
      721 cggactcggc ttcgtcgagg aataccgccg cgtctatccg atgggcgcca cggccggtca
      781 tgtggtcggg ctcgtcgacg tcgacagcaa gggtctcgcc ggcatcgaga aattcatcga
      841 cgacaatccc cagctcgcca tgatcggcgc ggagggggtc acgctttcgc tcgaccttgg
      901 cgcgcaacat gtgctgcgcg aggaactcgg ccgcgccatc ggcacctatc gcgccaaggc
      961 ggcggcggga atcgtcctcg acgtgcattc cggggaagtg gtggcgctcg tctcgctccc
     1021 cgactacgac ccaaatcgcc gcgagcaggc cctcgacaag gaaaggctca atcgcatcgg
     1081 cttcggcgtg tacgagatgg gctcggtgtt caaggtgttc accgtcgcgg gcgtgctcga
     1141 cggtggccaa gcaacgctgc gatcgcacta cgacgcctcg agccccatcc actacgcgtc
     1201 cttcaccatc gacgacttcc acggcaagag gcgcagcctc accgtgcccg aggtcttcat
     1261 ctattcgtcg aatatcggcg cggccaaaat ggcgctcgac atgggcgtcg agcgccaccg
     1321 caactttctc aagaagctcg ggctgatgag ccgcgtcacc accgagcttg gcgacagcgc
     1381 ttcaccgatc gttccgcagc actggcagaa actgaacacc atgaccatcg ccttcgggca
     1441 cgggctctcg gtgaccccgc tccagcttgc cgcggcctca cagcctctcg tcaatggcgg
     1501 gatcgccgtg acgccgacat tcctgccgcg ctcgcgggag gagggcttga gcggcggcaa
     1561 aagagtcctc aagcgcgaaa cgagcgccgc catgctgaag ctgtggcgcc tcaacgtgct
     1621 gaaaggaacg ggcaagcacg ccgacgccga aggctatcgt gtcggcggca agacgggcac
     1681 ggcagagaag gtcgtgggcg gccgctattc cacttccgcg ctgctgacga gtttcctcgc
     1741 cgccttcccc accgacgcgc cggaatatct cgttctggtc atgctcgatg agccgcaacg
     1801 cgtggcggaa tcgaacaatc aggcgacggc cggcgtcaac gccgcgccga ccgccggcaa
     1861 gatcatcgag cggattgcgc ccatcctggg cgtcgcgccg aaactcgagc gcgatcagcg
     1921 caaatttgac gccaaggtgc tcgcctccta ttaacagacg cccaccccat cgggttcgcc
     1981 aaatccttga tcctcaagga aatgccactt ttcggggcgc atgttcgacc atgactctgg
     2041 gtgagctctt cgggcgcgac gcgaagctgc ccgccggcct cgaacgcgtg ccgatcgagg
     2101 gccttgcggc cgacagccgc gacgtgaggc ccggctatct cttcgccgcg cttccaggcg
     2161 taaagacaga cggcgcgcgt ttcatcgccg atgcgttgca acgcggcgcc gcggcgatcc
     2221 tcgtgccgca cggcgcaaag ccgccaaccg gttccgccgc gatcatcgag gacagcgatc
     2281 cgcgccggcg ccttgcgctc atcgccgcgc gcttctttgc tctccagcca cagacgaccg
     2341 tcgccgtgac cggcaccaac ggcaagacgt cagtcgcgtc cttcgtgcgc cagctttggt
     2401 cgggggaggg tttcaaggcc gcgagcctcg gcaccgtcgg cgtggtgagc ccctcgggca
     2461 ccaagacgct gatccacacc acgcccgatc cgatcgagct tcaccgcatc ctcgccgctc
     2521 tcgccacaga gggcgtcacg catctcgcgc tggaagcttc cagccacggc ctgcaacagc
     2581 gccgcgtcga tggcatcgtg ttcgccgccg gcgccttcac caacatctcg cgcgatcatt
     2641 tggactacca tgcgagtttc gaagactatt tcgcgcagaa actccgcttg ttcaccgagc
     2701 ttctgccgcg aggcgcaacg gccgtggtcg acatcgacag cgatgcgggg aagcacattg
     2761 ccgcggtggc ggaagcgaag gggctcgatc ttgttacggt cggacgcgag ggaaagacct
     2821 tgcggctcgt ctcggccgcg cgcgacggct tcggtcaacg tctcatcgtc gagcatggcg
     2881 gcaagcagca ccaagtacgc ttgccgcttg tcggcgcctt ccaggcgtcg aacgcgctcg
     2941 tcgccgccgg gctcgtcatg gcgaccggca cgagcgctgc cacggttctg ccgatgctcg
     3001 aggcgctgca aggcgcgcgc gggcggctcg atcttgccgg cacggcgcgg gccggggcgc
     3061 cgatcttcat cgactatgcg catacccccg acgcgctcgc gaaggcgctt gacgcgttgc
     3121 gcccctatgt cgagaggagg ctcgtcgtcg tgttcggctg cggcggcgac cgcgacacgg
     3181 gcaagcgccc ggagatggga cgggttgccg tgacgaaagc cgatctcgcc attgtcaccg
     3241 acgacaatcc gcgcagcgaa attcccgccg cgatccgcag ccagatcttg caggccgcgc
     3301 ctggcgcgct cgagatcggc gaccgggccc gcgcgatcac ggaagccatc ggcacgctgg
     3361 agcggggcga tgtgctgctg atcgccggca agggtcatga gacgggccag acgattggca
     3421 cgaaagtgat cccgttctcg gatcatgacg cagtggccgc cgcgctgaac caagaggtca
     3481 agtgtggctg agccgttgtg gacccttggc gagatcgttc acgccacgcg cggtgcgtgc
     3541 ctccgcgcgg aaggagcgcc ggtgtttggc ttctccatcg atagccggag cctcgcgccg
     3601 ggagaggcct ttatcgccat ccgtgggccg aaccgggacg ggcatgcatt tgtcgccacg
     3661 gccctcgata agggcgcgac ttgcgcaatc gtggacaaga ccttcccgcc cggcgacgag
     3721 caaaaattgg tccgtgtcgg cgatacgctg gaagccctga acgatctggg acgcggcgcc
     3781 cgaacgcgcg cgagccgcac caaagtgatt gccgtcaccg gcagcgcggg caagaccggc
     3841 acgaaggaag cgttgcgctt agcgctcgcg cccagcggct cggtgcacgc ctctgccaag
     3901 tcgttcaaca accattgggg cgttcccttg tcgcttgcca acatgccgag gaccgtgcgc
     3961 ttcggcgtgt tcgagatcgg catgaatcac gcgggcgaaa tcgacgcgct gacgcggctc
     4021 gccaggcccc acatcgccat cgtcaccacg gttgcgccgg tccatctcgg cttcttcccc
     4081 tcggtcgaag cgatcgccga tgccaaggcc gagatcttca gcgggcttga accgggcggt
     4141 acggcgatca tcaaccgcga caacccctat tacgagcggc taaagcatca tgcgctgaac
     4201 cacggcgcca ccatcgtcgg gtttggcgag gcgcgagatg ccgaagcgcg gttgcgcgac
     4261 gtcacgctcg atcccgacgg atcgaatgtc agcgccgaca ttctcggcga gaccatcgcc
     4321 taccggctcg gcgcagccgg ccgtcatgtc gtgcagaact cgcttgccgt gctggcggcg
     4381 gccaaggtgg caggcgctga ccttgcgctc gccgccggtg ccctagccgg gcttgaggcg
     4441 caagccggtc gcggcgcccg caacgtgatc gagaccgaag gcggccgcat ggccatcatc
     4501 gacgaatcct acaatgccaa tccggcctcg atgcgcgccg cgctcgcaac gttggggctc
     4561 accccacgga gtgcgtttcg ccgccgcatc gccgtgctcg gcgacatgct cgagcttggg
     4621 ccggagggat ttcggcttca ccgagagctt gcggagttca ttgacggggt gggggtagat
     4681 gttgtctttg cctgcggcga acagatgggt tcgcttttcg aggctctgcc cgcaagccgc
     4741 cggggcgctt atgcgaaaac cgccgaagag cttgggccca agctccttga agcggtcggc
     4801 ccgggggacg cgatcatggt taagggctct ctcggcagcc gcatggcgcc tctcgtcgag
     4861 gcgttgaagc gccggttcgg gaaggaagca gcgccggcgt aaggcggggc cggggagggg
     4921 gagaaggcgg atgctttatt ggctggccgg gttcgcgcct gaactcccga tattcaatgt
     4981 cttccgctac atcaccttcc gcaccggcgg agcgctgctc acggctcttc tgttcgtatt
     5041 tctgttcggg ccgaaggtga tccggtcctt acgcatccgc cagggcaagg gtcagcccat
     5101 ccgcaaagag ggccccgagc gccatctcat ccagaagcag ggcaccccga ccatgggggg
     5161 gctcatgatc ctgtccggcg tgaccgtcgc cgtgctgctg tggggcaatc tgcaaaatac
     5221 gtttgtctgg gtggtgctgc tcctcaccct cgtttatggc gccgttggcc tctacgacga
     5281 ctacctgaaa gtgaccaagc agtcggcgca aggcttccgc gggcgcatca aaatcgtgat
     5341 cgagggggca gccgccctgg tggcgaccac cgtcattgtc tacgcgagcc aggagccgct
     5401 tgcgacctcg ctcaccattc ccttcatcaa ggatgcgctc atcaatctcg gctggttgtt
     5461 catacccttt ggcgctttca tcatcgtcgg cgctggcaat gccgtgaact tgaccgacgg
     5521 cctcgacggg cttgccatcg tgccggtgat gatcgccgcc gccacgttcg gcttcatctc
     5581 ttacctcgtc ggcaacgcgc tctttgccga ctatctgcag atccatttcg tgccgggcac
     5641 cggcgagctt gcggtgattt gcggcgcgct ggtcggcgca gggctgggct ttctctggtt
     5701 caacgcgccg cccgcgctca tcttcatggg cgacaccggc tcgctcgctc tcggcggcgc
     5761 gctcggaacc atcgccgtgg ccaccaagca cgaattggtg ctcgccatcg tcggcgggct
     5821 gtttgtgctc gaagccgtct ccgtgatcgt gcaggtcacc tcgttccgtc tgttcggcaa
     5881 gagggtgttc cgcatggccc cgctccacca ccattacgag cagaagggct ggccggaatc
     5941 gaccgtggtg atcaggttct ggatcattgc cgtggtgctt gcgcttgctg gtctcgccac
     6001 gctgaaattg cgctaaattc cctcattatg attcccgtga cgatctttgc cggccgggac
     6061 gttgcgatcc tgggcttggg gctcagcggg cttgcctccg cgcgcgcgct tgaagccggc
     6121 ggcgccactc ccatcctctg ggacgacaag gcgagcgcgc gcgaggaagc ggccggacag
     6181 ggcttcaccg tgagcgatct cagcgcggcc gactggtcgg gcttcgcggc gctggtgctc
     6241 gcacccggca ttcccttgac ccaccccgcg ccccattgga gcgtggcacg cgcgcggaac
     6301 gctggcgtcg aggtgatcgg cgatgtcgag ctgttcttcc gtgagcgcgc ccgcgcccgt
     6361 acgccgggga aagtcgtggc catcaccggc accaatggca aatcgaccac gaccgcgctc
     6421 gttgcccatc tgctcgccgc cgcgggaaag gccgttgcgc tcggcggcaa cattggcaag
     6481 gccgtgctcg acctcgatcc gttcgcgccc ggcctcacct atgtcctcga gctgtcgtcg
     6541 ttccagatcg agctcagccc gagcctctcg cccgacgccg cagcgctcct caacatcacc
     6601 ccggaccatc tcgaccgcca cgccacgctt gcgcattatg cccgcatcaa ggcctcggtc
     6661 ttcgccgggc ttggtgctcg cgccaccgcg gtcgtcggcg tcgatgacga ggtctcgcgc
     6721 gccatcgccg acggtctcga cgggccttat gccgtgaaac gcatcgcggt cggccgcgac
     6781 gtggagacgg gcgttttcgt cactgacggg atccttcatg agatggaggg gggcggcgag
     6841 aaggcgcgca tcgaccttgc cggcatcggc tcgctcaggg gcgcgcataa ttggcagaac
     6901 gccgcggccg cctacgcgct cgcccggtcg caaggactga gcccggccgc gatcgccgaa
     6961 ggcctcaaga gtttcgccgg cctctcacac cgcatggagc aggtggcgcg caggggcaag
     7021 gtgctgttcg tcaacgattc caaggccacc aatgccgacg ccgccggcaa ggcgctcgcg
     7081 agctttaccg atatatactg gattatcggc ggcaggccga aagagggggg attagcggga
     7141 cttgaacctt tctacccgag aatcgtccgc gcttatctga tcggggaggc ggcggaagcc
     7201 tttgccagcc agctcggagg tcacgtcgat catgtccatt gcggcacgct cgcccgcgcc
     7261 gtcgaagccg cggcttccga cgcgagccgc tcggccgcca aggagccgat cgtgcttctc
     7321 gcgcccgctt gcgcctccta cgatcagttc gacaacttcg ccaagcgcgg cgatgccttt
     7381 cgcgacctgg tcatgggtct cgacggggcc gaaggggtga ggcccgagca gggacaagcg
     7441 gcatgaggct gacacgcgca gaccgcagcg ttctttccga ctggtggttc accgtcgacc
     7501 ggctgatgtt cttcggtctg cttttgctca tgggcgccgg cctcgtgctc tcgctcgccg
     7561 caagcccgcc gatcgccgcc aaatacgatc tcgagccgtt ctatttcgtg cgccgccacg
     7621 ccgcgctgat cgtgcctgcg gtcgcgatca tgttcgcggc ctcgacgctg acgccgaagc
     7681 agatccggcg cgcgagcctc gtcattttcc tcgtcggcat cgccctgatg gcgctgatct
     7741 tcgggtttgg ccccgagatc aagggcgcca agcgctggct ccagattgga atcgtgtcga
     7801 tccagccgtc ggaattcgtg aaacccgcct tcgtcgtgct cacggcctgg ctgttcaatg
     7861 agagccagaa acggcgcgac gtgcccggca tcgagcttgg ggtggcgctt tacgcgatct
     7921 tcgccgcgct gctcgtgctc cagcccgatt tcggccagac gcttctcgtg acgctggtct
     7981 ggggcgcgct cttctatatg gccggcatca acatggtgtg gatcggcgcg ctcgccgtcg
     8041 ccggtatcgc cggtatcgtc gtcgcctatt tcctcattcc gcacgtcacc tcgcgcatcg
     8101 accgcttcat cgatcccgcc tccggcgaca cctaccaggc cgatcgctcg ctcgattcgt
     8161 ttcttcatgg cggctggttc ggccgcggtc ccggcgaggg cacggtcaag aacgtgcttc
     8221 ccgactccca caccgatttc atctttgccg tgaccgccga ggaatacgga ctgatcgcct
     8281 gcctcatcct gcttgtcctg tttgccttta tcgtgctgcg tggactgtcg aaagccgcgc
     8341 aagagccgga cggcttcatc aggcatgccg tcgcgggtct catcatgctg ttcggcttgc
     8401 aggccttgat caacatggcg gtcaatgtcg gactgctccc cgccaaaggc atgacgctgc
     8461 ccttcatctc ctatggcggc tcgtcgctgc tcgccatggc gctgaccatg ggcttcgcgc
     8521 tcggactgac gcgcaagcgg cccatggccg ggcgcctgcc ggcggccgac gcccatcgcg
     8581 acgccgagtc cgaacgcatc agggagcagg cggcttgacc gcgcgccctt tccttctcgc
     8641 cgcgggcggt accggcggac atctctttcc cgccgcggcg ctggcgcagg aactcaaacg
     8701 ccgcggctat gacgtcgagc ttgcgaccga catccgggcc gagaaatatg gcgtggagtt
     8761 tccggcgcgc gcgatccatc gcattccttc cgccacgctc acaagccgca atcctttcgc
     8821 cgtgaccgtg accttcacgc gtctcggcgt cggcttcttc ggcgcgcttc gccttctcat
     8881 gacgagcaag ccgcgcgcag tgatcggctt tggcggctat ccgacattgc cgccgatctt
     8941 ggccgcgcgc gcgagcggca ttccgaccgc gatccacgag cagaatgcgg tcatgggccg
     9001 cgcaaaccgg ctgctctcgc gcttcgtcga ccgcgtcgcg ctctccttca agccgacgag
     9061 attgcttcgc accgatgccg aagcgaaggc gcgcgtgacg ggaacgccgg tgcgcgatgc
     9121 cgtgctgtcc taccgcgagg ctccatatga gccgcccgag ccgggggggc cgctcttgct
     9181 cctcgtgttc ggcggaagcc aaggcgcgcg attcttctcc gaagctctgc cgcccgcgct
     9241 tgcgcggttg ccgaaggaga tgcgggagcg tctcactgtc gtgcagcagg cgcgcgagga
     9301 ggacatcgac gaattgcagg agggttacaa gaaggccgga atcgcggcgc acatcgcacc
     9361 ctttttccgc gatctgcccg agcggatcgc gaaatcgcat ctcgtcgtgg cgagggccgg
     9421 cgcctcgacc gtcgccgagc tgatggcgat cggcaggccc tgcctgctcg tgccgctccc
     9481 gcacgccctc gacaacgacc agctcgaaaa cgcaacgcgc ctgcaagaag gaggcggggg
     9541 ctggtgcgtg cgccagcagg agctcacccc cgaacgcctg gcgggcgagc tgcaaaggct
     9601 tctatcctcg cccgaggtct tggccgcgac cgccgcccga ggcaaggcca tggccgaaac
     9661 cgaggcggtg agcaagcttg ccgatctcgc cgaggagctc gcgcgcagca aagctgagta
     9721 gtgtagagtt cgagcatgca tcttcccgac ccggatcgag ccggcctgtt ccacgtcgtc
     9781 ggcattggcg gcatcggcat gagcgcgatt gccgaagtga tgcacacgcg cggctatcgc
     9841 gtgcagggaa gcgacctgaa ggacggcgcc aatctcgacc gcttgcgcgg acgcggcgtc
     9901 acctgcctga tcgggcatga tcccgccaac gtcacgggcg cgggtcatct cgtcatttcg
     9961 agcgcggtga agccgggcaa tccggagttc gaggcggcca aggagcgcgg catccccatc
    10021 atccgccgcg ccgagatgct ggccgagctg atgcgccctt gcgccaccgt gtcgatcacc
    10081 ggcacgcacg gcaagaccac caccacctcg ctcatcgccg acgtgctgaa atgcgccgac
    10141 ctcgatccga ccgtgatcgc gggcggcatc atcaacggct ggggcacgaa tgcgcgcatc
    10201 ggccggggcg aatggatggt ggtcgaggcc gacgaatccg acggcacctt cttgaagctt
    10261 cccacccaga tcggggtcgt gacgaacatc gatcccgagc acatggacta ttggccttcg
    10321 atcgaagcgc tgcaccagtc ctttctgcaa ttcatagagg cgatcccgtt ttatggcgtg
    10381 gccgtggcgt gcatcgatca tccggtagtg cgcgagctgc tggcaaagct tggaggcgcc
    10441 gccaatggac gccgcaccct gacctacggg gtcgcgcgcg atgccgatat caggctcggc
    10501 aatctgcgcg cgagccgcga ccggatcgta ttcgacgccg aactcggcca cgctgtgaga
    10561 ggcggcgcgc gcaagctcga agatctcatg ctgccggtgc cggggcatta caatgcgctg
    10621 aacgcgctcg gcgcgctagc tgccgcgacg gaagcgggcg tcagcgatga cgtgatccgc
    10681 aaggcgtttg cggccttctc cggcgtcaag cgccgcttca gccgggtcgg cgacttcgac
    10741 ggcgttagcg tctacgacga ttatgcgcac cacccggtcg agatcgcagc cgtgctccgg
    10801 gcggcgcgca tcggcggcga tgggcgcatc gtcgccgtgg tccagccgca tcgctacacg
    10861 cgtctgcaat ccttgttcaa cgagttctcg gcctgcctcg acgacgccga tgtcgtagtc
    10921 gtgacgccgg tctactccgc gggcgaagcg ccgatccccg gcatcgatcg cgacgagctt
    10981 gcgtcaggct tgcgccgcca cggccatcca aatgtgctga ccattgacga tgaagagacg
    11041 ctggtggaga cgatcgcctc tgtggcgaag cctggcgaca tcgtcgttgg cctcggcgcc
    11101 ggcaccatca ctgactggat gaacgctttg cccaagaagc tcgagcagca caggggacgg
    11161 gcttgaacgg gcgcgacctc gaaagccgct tgaccgcaag cctgcctgag ctgcgcggca
    11221 ggctgaaggc cgaagccccg ctcaaggacc tgacctggtt cagggcgggc ggacctgcgg
    11281 aggtgctcta ctctcccgcc gacgaagcgg acctcgccta tttcctgaaa gcaactccgg
    11341 ccgacattcc cgttaccgtc atcgggctcg gctccaatct tctcgtgcgc gacggcggca
    11401 tcgcgggcgt cgtgatccgg ctcggacgcg gtttcggcga gattgccatc gaagacggca
    11461 cgagacttcg ggccggcacc gccgtgcccg acgtcaaggt cgcgcgcgcc gctgccgaag
    11521 ccgggatcgc cggcctctcc ttctatcgcg gcatccccgg ttgcgtcggc ggcgcgctcc
    11581 gcatgaatgg cggcgctcat ggccgcgaga cgaaagaggt tctcatcgag gtgcgtgccg
    11641 tcgaccgcga gggcaacacg catgtgctgc ctgttgccga gcttggctac gcgtatcgca
    11701 gctgcggcgc gcccgaggac ttcatcttca ccgaggcgct gtttcaggga gaaccgggcg
    11761 accccgccaa gattctcgcc gagatggacg acatcgccgc ctatcgcgag gaagtgcagc
    11821 cgatcaagag ccgcaccggc ggttcgacct tcaagaatcc gccgggtcac aaggcgtggc
    11881 agctcatcga tcgcgccggc tgccgcgggc tctccgtcgg cgatgcaaag gtctccgaga
    11941 tgcactgcaa ttttctcatc aacgagggga atgccacagg cgccgacctt gagacgctcg
    12001 gcgagacggt gcgggcgcgg gtcaaggaga cgagcggggt cgagctcgaa tgggagatca
    12061 agcgcctcgg cattccggcg tgatcgccct cgacacgcca agcctgcact gcctcctcgc
    12121 cgcgaggcgt catgccgtct aggagccgtc caacaccgca gagagtaaga cctgctcgga
    12181 agtcagccgg gggaacgtca atggcaaaca agcaagttca cgtcgtggtg ctgatgggcg
    12241 ggctgtcggc tgagcgcgag gtctcgctcc gctccggcaa ggcctgcgcg gatgcgctcg
    12301 aagttcaagg ttatcgcgtg agccggctgg atgtcggcca ggacgtggcg gcgcggctcg
    12361 ccgagctggc gcccgatgtc tgcttcaacg ctttgcacgg gcgctatggc gaggatggct
    12421 gcatccaggg tgtgctcgag accatgggga ttccctacac ccattcgggc gtgcttgctt
    12481 ccgcgctggc gatgcacaag gagcgcgcca aggccgtgct cgccgcggcc ggcgttccca
    12541 ttgccgaaag cctggtgatc gcgcgcaccg aagcggcccg cgcgcacgcc atgcccccgc
    12601 cctacgtgat caagccgccg gccgaaggtt cgagcgtggg cgtcttcatc gtccgcgagg
    12661 accacgccca tcctccccag cagctgaccg cctccgaatg gaccttcggc gaggaggtca
    12721 tgatcgagcg cttcatcccg ggccgcgagc tgacctgtgc cgtcatgggc gaggagcctc
    12781 tcggcgtgat cgagatccgc ccggccgaag ggctcgcctt ctacgactac gacgccaaat
    12841 acgcgcccgg cggctcaatt cacgtccttc cggcgccgct taaaccaaat gtttaccaat
    12901 tggtacagaa gctgtcgtta gcggctcaca gggcactggg ctgccgtggg gtcagtcggt
    12961 ccgacttccg ctacgacgac cggcctgagg gcacggggga agtgatctgc ctcgaggtca
    13021 atacccagcc cggcatgacg tcgacgtcgc ttgtgcccga gcttgctgcc catagcggcc
    13081 gctcgtttgg cgagctggtg cgctggatct tggaggacgc gtcgtgcaac cggtgagggg
    13141 aatggcaagg agacggggct cgcgagagcc cctgttggcg cgcggcatcg ccgccccgcg
    13201 caaagtctcc agccgtttgc ctgtgctcgc cgaagcgcgc cgcctcatca cccgcagccc
    13261 gcgcacgatc aggctcggcc gcatcggggg ccatgcgcgc agcaaatggc ttgtcctctg
    13321 cgcgctcttc ctcggcagcg ccttggtcta cggcgccgtg atcggcggcc agaccggccg
    13381 cgcctacgac gctctcacca acggtatcaa tcgcctcgcc atcgccgcgg gcttcggcgt
    13441 caagaaaatc accgtcgccg gccagctgca tgtgaccgat gcgaccatca ccgcggcact
    13501 cggcgccgga cccgacacca tgatgctcgg cttcgacacc gacgcggcca aggagcggct
    13561 cgaagcggtg ccttggatca ggcacgccca ggtcatgcgc ctgctccctt cgaccttgca
    13621 ggtggtcgtg gaggaacgca cgccgttcgc ggtctggcag aaaaacggcc agacctttgt
    13681 cgtcgatccc gagggcgtcg tgctggcccc ggccttgcgt gacgcgtatg cggctcttcc
    13741 tctcgttgtc ggcgagggcg cgggcaagaa cgccgcccaa ctcgtcgagc agctggccgc
    13801 gcatgacgat ctcaagcagc accttctcgc cgccatcagg gtcggcgacc ggcgctggac
    13861 gctgaagctc atctcgggca ccgacatcat gttgcctgac gacaatgtcc ctgaggcgct
    13921 cgcttccctc gccaagctcg acggcgaacg cgaggtgctg aagcgggata ttgccgccgt
    13981 cgatctgcgt ctcctcgatc gaatcacggt gcgcctgcgc gcggccgagg aggcaaaacc
    14041 acaaggcgga acgccgcaag ccgacatgcc gaccgcgagc accaacgcgg cggccaaggg
    14101 aaagacgtga cgggataggg tttagggacc gggggatggg atacacgacc tcgataaggg
    14161 gcaagcgcca gcggatcgtg accgcgctcg acatcggcac gagcaagatc tgctgcctga
    14221 tcgccaaggc tcagccttcg cccgattggc tcgacggcag gggcgatgcc atgcagttcg
    14281 aggtgctcgg cttcgaccac accagggccg aaggcctcaa ggccggcatg atcgcccatc
    14341 tcgacagcgc cgagacgtgc atccgctcgg ccgtggatgc cgccgagcgc atggcgggcg
    14401 tcaccgtcga ggacgtgcag gtttcggtca cctgcggacg tctcaagagc gagacgttct
    14461 cggcgagcgt ggcgctcgct tcggggaccg tgcgtgaaga cgatatccgg cgcctgcttg
    14521 gcggcggccg ccaatatgcc gggcgcgaca agcggaatgt gctccatgcg ctgcctctaa
    14581 gctaccggct cgacgacaat gccggcatca gcgaaccgca aggcatgtgc ggcgaacgcc
    14641 ttgccgtcga tctccatgcc gtcaccgccg acgaagtggc gatgcgcaac gtgatgctgt
    14701 gcgtcgagcg ctgccatctc ggcgttgcca gcctcatcgc ggcgccctat gcgagcgcgc
    14761 ttgcggtggt cacgccggac gaggccaagc tcggcgtcgc ctgcattgat ttcggtgccg
    14821 gcacgacgac actctcggtc cttgccgacg ggcagttcat tcataccgat gccatcgcgt
    14881 ttggcagcaa cagcatcacc accgatatcg cacgcacgct cgcggcgccg ctcgattacg
    14941 ccgagcggct caagacgctg cacggggccg ccttcgccac gctgtccgac gagcgcgaga
    15001 tcatcaccta tcccgccgtc gccggcatgt cgcaggcgag cttgaaccag atcaccaagg
    15061 cacagctcgt cgccatcatt cgcccgcgcg tcgaggagac gctcgatctc ctgcggcggc
    15121 ggcttgccgc aagcggtatt gcggcggaag tgacgcagca tctcgtcctg acgggcggcg
    15181 ggagccaact gacaggtctg cccgagctca ccgccaacat gttcgggcgg cccgtgcgcc
    15241 tcgggcgccc gcgatctttg cgcgggctgc cgtccgcggc cgcatccccc gattttgccg
    15301 tcggcgtcgg cctcctgctc aattgggcga aaggcgacga cagacaatcg ggcgccgcgg
    15361 agcagcgttt tctgcgcacc ggcaccggtt atttcgccaa ggtcggcgaa tggataaggg
    15421 acaatttttg agcgttagag ggctcgagta gcgcctcgcg gggaagcgcg aaaacgagcc
    15481 ggagcaaggg caatgaagga tcgaaagacg gccatgacca tcaacctcgg agttcctgat
    15541 ctcactgaac tcaagccgcg catcaccgtg ttcggtattg gcggcgcggg cggcaatgcc
    15601 gtcaacaaca tgatcgcggc gggcctcaag ggcgtcgagt tcgtggtcgc caataccgat
    15661 gctcaggcgc tcgcgagctc gaccgctcag cgtcgaatcc agatgggcac ccacgtcacc
    15721 gaaggcctcg gcgccggatc gaagcctgag atcggcgcgg ccgccgccga ggaggccatg
    15781 gccgacatca aggcccatct cgtcggctgc cacatggtgt tcatcaccgc cggcatgggc
    15841 ggcggcaccg gaacgggcgc ggcgccgatc atcgcccgcg cttcccgcga ggagggcatc
    15901 ctgaccgtgg gcgttgtcac caagcccttc catttcgagg gcgcgcggcg catgcgcgcg
    15961 tccgaggtcg gcatcgagca gctgcagaaa ttcgtcgaca ccctgcttat cattccgaac
    16021 cagaacctgt tccgcgtcgc caacgagaag accactttca ccgatgcctt cgccatggcc
    16081 gatgacgtgc tgcgggccgg cgtgtcctgc atcaccgacc tcatggtcaa ggagggcctg
    16141 atcaatctcg acttcgccga tgtgcgcacc gtcatggccg gcatgggcaa ggcgatgatg
    16201 ggcacgggcg aggcgagcgg cgaccgccgc gccatcgacg ccgcggaagc ggcgatctcc
    16261 aatccgctgc tcgacgatgt ttgcatgagg ggcgcgagcg gactgctcgt gtcgatcacc
    16321 ggcggcaacg acctaaccct ttatgaggtc gacgaggcgg cgagccgggt gaggcaggag
    16381 gccgacgccg aagccaacat catcgtcggc gcaaccttcg acgacgagct tgacggatcg
    16441 atccgcgtgt cggtggttgc aaccggcatc ggcggggctc aggccgagat gcctcccgcc
    16501 aacccgcagg agcggaaccg gctgagcaag cggctcgcga gcctcgccac gctcccaggc
    16561 atgcctgtcg accacgacga accgagtctc gtcctggccg aggccgagat ggtcaaggag
    16621 gccccggtct ggcgcgggcc cggcaatgtg acgatcgaga aacgccccgc gcaggtcacc
    16681 gcgatcgggc cggtcggggt ctcgcagccg aagcaagcgc cgcaatcggc gtcgcggcct
    16741 ttcgagcccg cgcctcctgt cgggatcaag cgcgcggttc ggcgcatgcc gaccatggac
    16801 gaactgccgg tcgttaccca gaacgagatc aaggccaagt ctggggagac gcccgttatt
    16861 ggtcttgccg cgcaaaagaa gaaggtgggc ttcctcgagc gccttgccaa tgtcggccgg
    16921 tctcgcaagg agcccgacga gccgcccgcc aaacgggagc ccgagttcga gagaccctgg
    16981 ggcgagttgc cgaagatggc cgagcgcgca aagcccgagc ccgaagcggc ggcgccgaga
    17041 gggatccgga tcgagcgccc gcgcggcgag aaagtggtcg cggccctgcc gaaactggtc
    17101 gaggtccagc ctgcgctcga aacgcccgac gaatcaccgg ccgacgatga tctggagatt
    17161 cccgcgttcc tgcgccgccg cgcaaactga gcgctgctcg aaaggttaag aactccgtcc
    17221 gcgacacact ccggcgaagg agaaatacac agctacggca gcatgttatg aggtcccaga
    17281 gtccgtaata cagtgaaaga aaccgtgatt tgtcgcctcc gagtccgagc gttaaggttt
    17341 ctccagattg atttgcggga caacaaaggc acaccgcaag gactgacaaa aagtccggct
    17401 tcaagtgcca aggctcgctg atcgatcggg gggttcgagg gtctcacctc gaacaaagta
    17461 gggaactcaa aaatacggcg cgggggccga cgggggacat gaggggcttt atcggggcac
    17521 gccaaacgac gcttgccagt gagatcaccc ttaacggcac gggtgtccac agcggagcgc
    17581 cagtctccat cacactttgt ccggctgatg gcgatactgg tattcggttt cttctttcca
    17641 acggcaacgg ccacgagacc gagatcgcag ccgatcagcg gtcggtcgcg ggcgtaacgt
    17701 tgtgcaccgt tcttggcgac ggcaacggcg cctctgttgc gacggtggag catctgctcg
    17761 cggcgctgcg cgggctcggc gtcgataatg ccctgatcga gatcgacagc gccgaggtgc
    17821 cgatcatgga cggcagcgcc gcgcgtttcg tcgaagcgat cgacgaggtc ggccttgccg
    17881 agctcgagac tccacgccgc tatctcaaag ttctgaagaa ggtcgtggtc gaggatgccg
    17941 gcgcctatgg cgaattggct ccgcataacg gattccatct cgacgttgag atcgatttcg
    18001 acacgccgct gatcgggaac cagaggatcg agatcgacct gaaccccggc tcgttccgcc
    18061 gcgaactcgc ccgggcccgc accttcggct tcatgaagga cgtcgagcgc ctttgggcgg
    18121 ccgggcttgc tctcggcgcc tcactcgaca acaccgtcgc catcggcgac gaccgcgtca
    18181 tcaatcgcga aggtcttcgc ttctccgatg agttcgtgcg ccacaaggcg ctcgacgcgg
    18241 tcggcgacct tgctctcgcc ggcgctccca ttctcggcgc cttccgctcc caccggggcg
    18301 gccatcgcct gaacgcgctg gtgctcaagg cgctgtttgc cgatgccgag gcctggacca
    18361 tggtcgaagc cccgcgttac cgcgagattc gccatgccga gctcagccac cgtctcgcgg
    18421 cagcgaattt cgccgccgaa cggagctaga gaaggccggg aggcacacct cattcgcttc
    18481 gcgggtttcc gtctggccgt ctttgcgcgc gcggcacgcg cagattttcc gcgcgaccag
    18541 gaataggctg cgtagagggc cgactgacag ggcaaaaaaa caccataatc ggcgcgttgt
    18601 ttgcggggag ctgcgctatc atgacggcct ggttacttag ggcgtggaaa ccggtgcgct
    18661 aggggcagcc ttcgagggga agcatgagcg gacggcttga gcgcgaacga gcggcgacgc
    18721 ccccatcagg accgtctcgc gccgcgctcg cattgcctgc acgactggct tgcgtcctcg
    18781 ttgcggcgaa ccttctctcg gcttgcggtt cgttcggttc cttcggctgg ttcggtaaca
    18841 aggacgacga ggagaccgtc tccaacgatc cgccgaacgt catctacggc aaggccgaag
    18901 ccctcgtcga caggggcaaa tacaaggacg ccgcccgggc ctatgaagag gtcgacatca
    18961 atcaccccta ttcgccggaa gcgcgcaaag ccatcctcat ggccgcctac gcctattaca
    19021 agggcggcaa atatgacgac gcgatcgggg ccgccgatcg ctatctcacg cttcaccccg
    19081 gcacgcagga atcggatctc gcccagaaca tcatcgccat gtcctattac gatcgcgtgc
    19141 tcgatccgag gcgggatcag accaatgcgc gcaaggcgct cacggcttac gacacgctgc
    19201 tccagcgcta tcccgaatcg cgttacgcgg ccgatgcccg caaccgtggg cgcatcctgc
    19261 gcgatttgct cgcggccaac gagatgatgg tcggccgcta ctatctgcgc cacaacaact
    19321 tccttgcggc gatcaatcgc ttccgcaccg tggtcacgga cttccagacc accgagcaga
    19381 tcgaagaggc cttgatgcgc ctgaccgagg cctatatggc gttaggaatc gtcaacgagg
    19441 ctcagacggc ggccgccgtg ctcggccata actttccgga aagcaagtgg tatcgccacg
    19501 cttacggtct cctgggaaaa cgcggtctga agccgcagga gcacgaaggg tcctggatta
    19561 cccagacctg gaggggcgac ggcagacaaa cctgaccggc gcgccatgct agaggccctg
    19621 gcgatccgcg atatcgtcct gatcgacaaa ctcgatctcg aattcggcga gggcctgtcc
    19681 gtgctcaccg gggagaccgg tgccgggaaa tcaatccttc tcgatgcatt ttcgctcgcg
    19741 cttggcggca ggggcgatgc cgcgctggtg cggcgtgggg cgaaccaggg tcaggtgacg
    19801 gccatcttcg cgctcgatcg gagccacccg gtctcggcgc tgctcgccgc caacggcatc
    19861 gagcccgaga cggctctgat cttgcggcgg gtgcaaggcg ccgac
//
LOCUS       DASUQP010000016         7340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_144173,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000016 DASUQP010000000
VERSION     DASUQP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144173"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VFY74_00725"
     CDS             <1..1068
                     /locus_tag="VFY74_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5930266.1"
                     /translation="EYIPLPLIEQMAGLGVFSLTLPEEYGGLGLGKEAMCVVSEELSR
                     GYIGVGSLGTRSEIAEELILNGGTDAQKEEWLPKIASGEILPTAVFTEPNTGSDLASL
                     STRAVRDGDVYRLTGQKTWITHAARADLMTVLARTDPKEKGYRGLSMFLAPKPRGSDD
                     NPFPADGMTGGEIEVLGYRGMKEFDISFDGFAVPAANLLGGVEGQGFKQLMQTFEAAR
                     IQTAARAIGVAQSALDLGLRYGMERIQFGKPIIEFPRVSDKLAMMAAEILLARQLTYF
                     AAREKDAGRRCDLEAGMAKLLAARAAWAAADNALQIHGGNGFALEYPVSRVLCDARIL
                     SIFEGAAEIQAHVIARRLLEG"
     gene            1132..1578
                     /locus_tag="VFY74_00730"
     CDS             1132..1578
                     /locus_tag="VFY74_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX5930267.1"
                     /translation="MAGAACFAAAAIGRLASEPNLSWYETLEQPGFTPPNGIFPIVWA
                     ILYAMMAIAAWQFWRAEGKPEDRKLGLIWFGIQLLLNAAWPFAFFWLRSPGAGLVVIL
                     LLLIAIVATIVMFDWSSRSAALLLVPYLLWVCFAASLNFAFWFLNR"
     gene            1578..2402
                     /locus_tag="VFY74_00735"
     CDS             1578..2402
                     /locus_tag="VFY74_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5930268.1"
                     /translation="MGERSVLITGCSSGIGLASAREMKSRGWRVFATARKPGDLARLN
                     VEGFESLYLDYAEPDSIASAAEHVLRATDEKLGALFNNGGYGQPGAVEDLKPEILRAQ
                     FEVNVFGWHDLTRRIMPAMRKQDTGRIVFCSSVLGLVSAPYRGAYCASKFAVEALTDA
                     LRMELAATGIRVVLIEPGPIATRFLENAVAVYRRHIDLEASPHRDIYRLRIARMEEGG
                     SQTFKLGPEAVALKLAHALESRNPKRRYYVTIPSYAVALLRRILPAGALDAIAARN"
     gene            2443..2634
                     /locus_tag="VFY74_00740"
     CDS             2443..2634
                     /locus_tag="VFY74_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX5930269.1"
                     /translation="MEMLENYAIPIALAAVATVLFLGLWNMLRGGSPNTSQLLMRWRV
                     VLQFIALCLVMAAIYLAGR"
     gene            2726..3304
                     /locus_tag="VFY74_00745"
     CDS             2726..3304
                     /locus_tag="VFY74_00745"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5930270.1"
                     /translation="MVVLNKIYTRTGDDGTTALGSGRRVAKYDLRVEAYGTLDETNAA
                     IGLVRLHTRAGHPRLDAMLARIQNDLFDLGADLCYPDETRDARGRLQVTDAQVKRLEN
                     EIDTLNGELEPLRSFVLPGGSSAASFLHLARTISRRAERLIVALSAREGATIGAPALR
                     YINRLSDFLFVAARFANDKGKSDVTWVPGQNR"
     gene            3324..4034
                     /locus_tag="VFY74_00750"
     CDS             3324..4034
                     /locus_tag="VFY74_00750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5930271.1"
                     /translation="MFIPLHDTNPLKRIRFQYVTVALIALNVAIYVIFISGWLVPPNE
                     EEAASFAVIPAKLMTDGIDGRMMFATGAYMPERFTLVSYMFVHGSWIHLLGNMLFLWV
                     FGDNIEDAMGHMRFIMFYLMCGIFAAVLHSWMNPTSEIPLVGASGAVAGVIAAYLILH
                     PKVKVWVLALWRIPIRITAAWALGIWVAWQFANLLFEGEENVAWWAHTGGLLAGATLI
                     LFMRRRGVVLFDRTRGGA"
     gene            4142..4891
                     /locus_tag="VFY74_00755"
     CDS             4142..4891
                     /locus_tag="VFY74_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5930272.1"
                     /translation="MKILVPVKRVVDFNVKIRVKSDGSGIDLANVKMSMNPFDEIAVE
                     EAIRIKERGEADEVVAVSIGSGKSQDTLRTALAMGADRAILVKTEQNVEPLAVAKLLK
                     RVAETEKPDLIILGKQAIDDDCNQAGQMLAGLLGWPQGTFASKVELNGAKVKVVREVD
                     GGLETVMLSKPAVVTTDLRLNEPRYASLPNIMKAKKKPIDETTPEALAVDIAPRLKVV
                     SIAEPKGREAGIKVDSVAALVDKLKNEAGVL"
     gene            4893..5837
                     /locus_tag="VFY74_00760"
     CDS             4893..5837
                     /locus_tag="VFY74_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5930273.1"
                     /translation="MAVLLLAEHDNKTLSPATAKALTAARAFGGDVDILVAGENCRSV
                     AEAAAKLDGVRKVLLAEAPHLAQRLAEETASTVLPLMKEHSVLLAPATTMGKNVLPRI
                     AAMLDVAQVSDIVEVKAPNIFVRPIYAGNALETVETSDKIVVATVRTTAFASAGEGGA
                     AAIETIAPAPALSLSSYEGSEISVSERPELTAARIVISGGRGMQSGENFKTLIEPVAD
                     RLGAAVGASRAAVDAGYMPNDHQVGQTGKVVAPELYIAVGISGAIQHLAGMKDSKVIV
                     AINKDADAPIFQVADYGLVGDLFTVLPELDAELKKVGY"
     gene            5864..6805
                     /locus_tag="VFY74_00765"
     CDS             5864..6805
                     /locus_tag="VFY74_00765"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX5930274.1"
                     /translation="MDDIVQGAALRADPASRGDSDGDPIHKIGIVGAGQMGTGIAHVV
                     ALSGYDVALHDIAKDKVDAALGMIDRNLTRQVASGKITEDDKRKALSRISYAETMSRF
                     GDSDLVIEAATEDETVKRKIFVALCPFLKPNAIVSTNTSSISITRLAAATDRPERFIG
                     LHFMNPAPVMELVEMIRGIATEDATFGRTREFVETLGKTVAVSEDFPAFMVNRILLPM
                     INEAVYTLYEGVGTVDAIDTAMRLGAHHPMGPLQLADFIGLDTCLSIMQVLYEGLADS
                     KYRPCPLLVKYVEAGWVGRKSKRGFYDYRGDKPMPTR"
BASE COUNT         1301 a         2367 c         2292 g         1380 t
ORIGIN      
        1 gagtacatcc ctctgccgct gatcgagcag atggcggggc tcggcgtgtt ctcgctgacc
       61 ctaccggagg aatatggtgg ccttggcctc ggcaaggagg cgatgtgcgt cgtctccgag
      121 gagctgtcgc gcggttatat cggcgtcggc tcgctcggca cgcgctccga gatcgccgag
      181 gagctgatcc tgaacggcgg caccgacgcg cagaaggagg aatggctgcc gaagatcgct
      241 tcgggcgaga tcctgccgac cgccgtgttc accgagccca ataccggctc agacctcgcc
      301 tcgctctcga cccgcgcggt ccgcgacggc gatgtctatc gcttgaccgg acagaagacg
      361 tggatcaccc acgccgcccg cgccgatctc atgacggtgc tcgcgcgcac ggacccgaag
      421 gagaagggct atcgcgggct gtcgatgttc ctcgcgccga agccgcgcgg cagcgacgac
      481 aatcccttcc cggccgacgg catgaccggc ggagagatcg aagtcctcgg ctatcgcggc
      541 atgaaagagt tcgacatttc cttcgacggg tttgccgtgc cggcggccaa tctgctcggc
      601 ggagtcgagg gccagggctt caagcagctg atgcagacgt tcgaggcggc gcgcatccag
      661 acggcggcgc gtgccattgg cgtggcgcaa tcggcgctcg atctcggctt gcgctacggc
      721 atggagcgca tccagttcgg caagccgatt atcgaattcc cccgcgtgtc ggacaagctc
      781 gcgatgatgg cggcggaaat cctgcttgcg cgacagctca cctatttcgc cgcgcgcgag
      841 aaggatgcag gccggcgctg cgatctcgag gccggcatgg ccaagctgct tgccgcgcgc
      901 gccgcctggg ccgccgccga caacgcgctc cagatccatg gcggcaacgg cttcgcgctc
      961 gagtatcccg tgagccgggt cctgtgcgac gcccgcatcc tctcgatctt cgagggcgcg
     1021 gccgagatcc aggcccatgt cattgcccgc cgcctgctcg aggggtgagc ggccgcgcga
     1081 gttggttggc atgaaggcaa ccaataccaa cggaacttcc tcgctctctt cgtggcgggc
     1141 gccgcctgct tcgccgccgc ggcgatcggc cgcctcgcga gcgaacccaa tctctcttgg
     1201 tatgagacgc tggaacagcc cggcttcacg ccgccgaacg gcatcttccc gatcgtttgg
     1261 gcgatcctct acgccatgat ggcgattgcc gcctggcagt tctggcgcgc cgaaggcaag
     1321 cctgaggacc gcaagctcgg tctgatctgg ttcggcatcc agcttcttct caacgccgca
     1381 tggccgtttg ccttcttctg gttgcgcagc ccgggtgccg gacttgttgt cattctcctg
     1441 ctgctcatcg ccatcgtcgc caccattgtc atgttcgact ggtcgagccg gtcggccgcc
     1501 ctcctgctcg tgccgtatct gctgtgggtc tgtttcgccg catcgctgaa tttcgccttc
     1561 tggtttctca atcgctgatg ggcgagcgct cggtcctcat caccggttgt tcttccggca
     1621 ttggcttggc ttcggcgcgc gagatgaagt cgcgcggctg gcgcgttttc gccacggcac
     1681 gaaagccggg cgatctcgcg cggctgaacg tagaggggtt cgaaagcctc tatctcgatt
     1741 acgccgagcc cgactcgatc gcctccgcgg ctgagcacgt gcttcgggcg acggacgaaa
     1801 agctcggggc tctcttcaac aatggcggct atggccaacc cggggccgtc gaggatctga
     1861 agcccgaaat cctccgcgcc cagttcgagg tcaatgtgtt cggctggcac gatctgaccc
     1921 gccgcatcat gccggccatg cgcaagcagg acacggggcg gatcgtgttc tgctcatcgg
     1981 tgcttggcct cgtctcagcc ccctatcgcg gcgcctattg cgcctcgaaa ttcgcggtgg
     2041 aggcgctcac cgatgcgctc cgcatggagc ttgcggcaac cggcatcagg gttgtgctca
     2101 tcgagccggg gcccatcgcc acgcgtttcc tcgagaacgc ggttgccgtc tatcgccgcc
     2161 acatcgatct cgaagcttcg ccccaccgcg acatctatcg ccttcgcatc gcccgcatgg
     2221 aagagggcgg cagccagacc ttcaagctcg gccccgaggc ggtcgcgctc aagcttgcgc
     2281 atgcgctgga aagccgcaac ccaaagcgga ggtattacgt tacgattccg agttacgccg
     2341 tggccttgct gcgccgcatc ttgcccgccg gcgcgctcga cgccattgcc gctcggaact
     2401 agagtaccgc tcgcggggaa ggtcgccagg ggttcgcagg tcatggaaat gctcgagaat
     2461 tacgccattc cgattgcgct cgccgcggtc gccacggtgc tcttcctcgg gctgtggaac
     2521 atgctgcgcg gcggcagccc caataccagc cagctcctga tgcgctggcg cgtggtgttg
     2581 cagttcatcg cactctgcct cgtcatggcg gcgatctatc tggcgggccg ataggcgtcg
     2641 ttgtcacgaa ggcttgaccg gcctcgacgg ccagctacgc tagggtaagg aacaggccca
     2701 agggccctgg tttccgagac ggtgtatggt tgtcctcaac aagatctaca cgcgcaccgg
     2761 cgacgacggt acgacggcgc tcggctcagg caggcgcgtg gcgaaatacg atctcagagt
     2821 cgaggcctac ggcacgctcg atgagaccaa tgccgcgatc gggcttgtac ggcttcatac
     2881 ccgggccggg catccccgcc tcgatgccat gctcgcgcgc atccagaacg accttttcga
     2941 cctcggcgcc gatctttgtt atcccgacga gaccagggac gcgcgcgggc gcctgcaagt
     3001 gaccgacgcg caagtcaagc ggctggagaa cgagatcgac acgctgaatg gcgagcttga
     3061 gcccttgcgc tccttcgtgc tgccgggcgg ctcgtccgcc gcgagctttc tgcacctcgc
     3121 ccgtaccata tcgcgccggg ccgagcgcct tatcgttgcc ctttccgcac gcgagggtgc
     3181 gacgataggc gcgccagccc ttcgctatat caaccgcctg tcggatttcc tgttcgtcgc
     3241 ggcgcgcttc gccaatgaca aaggaaagtc cgacgtgacc tgggtgcccg gccaaaaccg
     3301 ctgacgcggg ggacgaagta tccgtgttca taccccttca tgacacgaac cctttgaagc
     3361 gcatccgctt tcaatacgtg acggtggcgc tgatcgcgtt gaacgtcgcc atctacgtga
     3421 ttttcatatc cggatggctg gtccctccca acgaggagga ggcggcttcc ttcgccgtca
     3481 tccccgcgaa gctgatgaca gatggcatcg atggccgcat gatgtttgcg accggcgcct
     3541 atatgcccga gcgcttcacc ctcgtctcct acatgttcgt gcacgggagc tggatccatc
     3601 tcctcggcaa catgctgttc ctgtgggtgt tcggggacaa tatcgaagac gccatggggc
     3661 atatgcgctt catcatgttc tatttgatgt gcggcatctt cgcggctgtg ctccattcct
     3721 ggatgaatcc cacatcggag atacctctgg tcggggcttc gggcgcggtc gcgggcgtga
     3781 tcgcggccta tctgatactg catcccaagg tgaaggtttg ggtgcttgcg ctgtggcgca
     3841 ttccgatcag gatcacggcc gcgtgggcgc tcgggatctg ggttgcctgg caattcgcta
     3901 atcttctgtt cgaaggcgag gagaacgtgg cctggtgggc ccataccggc gggctgttgg
     3961 ccggggcaac cttgattctg ttcatgcggc ggcgcggtgt agtcctgttc gaccggaccc
     4021 gcggcggggc ctaggagccc ggccaaaccc cggaaaccat tgacttgctc tggtcgcggc
     4081 agtacggtcg cggccatttc gcagttgcga gatagctgta gcgtgcgggg aacaaggccg
     4141 catgaaaatc ctcgttcctg tcaagcgggt cgtcgatttc aacgtcaaga tccgcgtcaa
     4201 atcggatgga agcggcatcg acctcgccaa cgtcaagatg tcgatgaatc cgttcgacga
     4261 gatcgcggtc gaggaggcga tccgcatcaa ggaacgcggc gaggcggacg aggtcgttgc
     4321 cgtctcgatc ggcagtggca aatcccaaga caccttgcgc accgcgcttg cgatgggcgc
     4381 cgaccgcgcg attctcgtca agaccgaaca gaacgtcgag ccgcttgccg tagccaagct
     4441 gctcaagcgc gtggcggaga ccgagaagcc cgatctcatc atcctcggca agcaggccat
     4501 cgacgacgat tgcaatcaag cgggtcagat gctcgccgga ctgctcggct ggccgcaagg
     4561 gactttcgcc tcgaaggtcg aactcaacgg cgccaaggtg aaagtggttc gcgaggtcga
     4621 cggcggtctg gagacggtca tgctgtcgaa gcccgccgtg gtcaccaccg atctcaggct
     4681 gaacgagccg cgctatgcct ccctgcccaa tatcatgaag gcgaagaaga agccgatcga
     4741 cgaaaccacg cccgaggcgc ttgcggtcga tatcgcgccg cgcctcaagg tcgtgtccat
     4801 cgccgagccc aagggccgag aggccggcat caaggtcgat agcgttgccg cactcgtcga
     4861 caaacttaaa aacgaggcag gggtgctttg aaatggcggt cctcctgctc gctgagcatg
     4921 acaataagac cctgtcgccg gccacagcca aggcactgac ggcagcgcgg gccttcggcg
     4981 gcgatgtcga tattctcgtc gccggtgaga attgccggtc cgtcgccgaa gcggcggcca
     5041 agctcgacgg cgtgcgcaaa gtcttgctcg cggaggcccc gcatctcgcg caacgcctgg
     5101 cggaggagac ggcaagcact gtactgccgc tgatgaagga gcactcggtt ctgctcgcgc
     5161 ccgcgacgac catgggcaag aacgttctgc cgcggattgc cgcgatgctg gatgtcgctc
     5221 aggtctccga cattgtcgaa gtcaaggcgc cgaacatttt tgtccgtccg atctatgccg
     5281 gcaacgcgct cgagacggtc gaaacctccg acaagatcgt ggtcgccacc gtgcgcacca
     5341 cggccttcgc cagcgccgga gagggcggcg ccgccgccat cgagacgatt gcgccggcgc
     5401 ccgccctgtc cctttcgagc tacgaagggt cggagatcag cgtgagcgag cggcccgagc
     5461 tgaccgcggc ccgcatcgtg atttcaggcg gacgcggcat gcagtcgggc gagaatttca
     5521 agaccttgat cgagcccgtg gccgaccgcc tcggcgccgc ggttggcgcg agccgggccg
     5581 ccgtcgatgc cggctacatg ccgaacgacc atcaggtcgg ccagaccggg aaggttgtgg
     5641 cgcccgagct ctatatcgcc gtcggcattt ccggcgccat ccagcatctc gccggcatga
     5701 aggattccaa ggtgatcgtc gccatcaaca aagacgcaga cgcccccatc ttccaggtcg
     5761 ccgattacgg tctcgtcggc gaccttttta ccgtcctgcc cgagctcgac gccgagctca
     5821 agaaggtagg gtactagtgg aacaggattt cagaggaatg accatggacg acatagttca
     5881 aggcgccgcg ctccgcgccg atccggcctc ccggggcgat tctgacgggg accccatcca
     5941 taagatcgga attgtcggcg ccgggcagat gggaacaggc atcgcccatg tcgtcgcgct
     6001 ctccggctac gacgtggctc tgcacgacat cgccaaagac aaggtcgatg cggcgctcgg
     6061 catgatcgac aggaacctca cccgccaagt ggcctccggc aagatcaccg aggacgacaa
     6121 gcgcaaggcg ctgtcacgca tctcctatgc cgaaaccatg tccaggttcg gcgactccga
     6181 cctcgtgatt gaggccgcga ccgaagacga gacggtaaag cgcaagatct tcgtggcgct
     6241 ttgtcccttc ctcaagccca atgccatcgt ctcgaccaat acctcgtcga tctcgatcac
     6301 ccggcttgcg gccgccaccg accgtcccga acgtttcatc gggctgcact ttatgaatcc
     6361 ggcgccggtg atggagctgg tcgagatgat ccgcggcatt gccaccgagg atgccacctt
     6421 cggccgcacc cgggagttcg tcgagaccct cggcaagacc gtcgccgtat cggaagactt
     6481 cccggccttc atggtgaacc gcattcttct gccgatgatc aacgaggcgg tctacacgct
     6541 ctatgagggc gtgggcacgg tcgacgccat cgacaccgcc atgcggcttg gcgcccatca
     6601 tccgatgggc ccgctccagc ttgccgattt catcgggctc gacacctgtc tctcgatcat
     6661 gcaggtgctg tatgaggggc tcgccgattc caaatatcgc ccctgtccgc tgctcgtgaa
     6721 atacgtcgaa gcgggctggg tcggacggaa gtcgaagcgc ggattctacg attatcgcgg
     6781 cgacaagccg atgccgaccc gctagaggcg gaccgcagtc gcgccgcttc acgccggacc
     6841 gcagtcgcgc cgccgctttg attcatcgaa cacgatttgc tcgaccgcgg gagcctcgac
     6901 gctcgcgccc catcgcgctt gtcgcaacgc cctcgatggc ctcgcatgac atggcaccgc
     6961 ctggattttg tccgtagcca tgacgacggt tggacaacat gactaaaaac tgaagcgaat
     7021 ctgcctcttt ccgacagcat tgcgtcatcc cgccggtagg catgaattga gcgcgttcta
     7081 gagggatccc tggcatgaga cgtacaaaga ggtactcggg tggacggcag cgtgggtcct
     7141 gtccgggccc cgttgagttg gtttccgacg tcctgccccg agtgaattga aagggatcag
     7201 ataggaaaga caatgcagca catgcgaggc ctcacccgca caggccgctg ctccttaagc
     7261 ggaaagtaat ggcttgcggc gcataaacca actccctccg tccaacctgg acggcggagt
     7321 tacccacttt ttggagttgt
//
LOCUS       DASUQP010000017         5492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_553108,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000017 DASUQP010000000
VERSION     DASUQP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553108"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..473
                     /locus_tag="VFY74_00770"
     CDS             261..473
                     /locus_tag="VFY74_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930275.1"
                     /translation="MNTKTALISAMIEIRGPRDGEFTVKFTAADGRSLAIHVPVQQGS
                     VLRDIQESMPYGIAVHDLVAVEVEAE"
     gene            complement(704..780)
                     /locus_tag="VFY74_00775"
     tRNA            complement(704..780)
                     /locus_tag="VFY74_00775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(744..746),aa:Arg,seq:acg)
     gene            complement(1054..2514)
                     /locus_tag="VFY74_00780"
     CDS             complement(1054..2514)
                     /locus_tag="VFY74_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5930276.1"
                     /translation="MKDENPDTTPEKPPMFRFVPGLSAKLLLLTIVFVMLAEVLVFVP
                     SVSNFRRQWLMERLSAAQIASLAAEAAPGGQLPSMLRDELLERAQVKAIAVKRDASRT
                     LIIEMDMPAEIDAAYDLRDASWLKLIADALMVYVAPEDRVIRVIGDPGFHEGESIEVV
                     MGEAPLKAAMIRYGLDILGLSILISIITATLVYLTLDALLIKPMTKLTWNIVRFSQRP
                     EDPTRVIAPSARRDEIGTAERELSAMQKELAETLSQRSRLAALGLAVSKISHDLRNML
                     SSAQLLSDRLITVKDPTVQRLVPKLIASLDRAIRLCAQTLNYGQVQEPPPRRKRFRLR
                     PLVTEIGDSLGLPRQRIGWSVEVEAMLEVDADRDQLYRVLSNLCRNAAQALESEAGME
                     GEIVVSGRREGAVAVIEVADTGPGVPDKARAHLFAAFQGSVRKGGTGLGLAIAHELVQ
                     AHGGQIALVENEGGATFRLTIPDMVVELKKARRLSA"
     gene            complement(2562..3986)
                     /locus_tag="VFY74_00785"
     CDS             complement(2562..3986)
                     /locus_tag="VFY74_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5930277.1"
                     /translation="MEEVLKPDLCVIGAGSGGLSVAAAAAQFGVPVVLIEKGRMGGDC
                     LNYGCVPSKALLAAAKRAEAVRTASTFGIDVIEAQIDHRGVHDHVHGVIAAIAPNDSI
                     ERMTGLGVRVIQAQASFLDNVTLEAGRVLVRARRFVVATGSMPSIPPIPGLDEVPYFT
                     NETIFDVSERIQHLIVLGGGPVGLELAQAHRMLGCHVSVFDVESFLAHEDPEAAAVLI
                     KRLADTQVMLHPDTRVERIERTTQGISVHFTENGEPTVLKGSHLLVATGRKPNVKSLS
                     LERAGIAYDDHGIKVNHHLKTTNRRVYAVGDVIGGPGFTHLANYQAGIVIRNALFRLN
                     PDASAGLIPRAVYTDPELAQIGLTEAEARKRHRRIRIYRAPYTDNDRAQCERATEGFI
                     KVITRPNGTILGCTIVGAEAGELLQLWVVAMAKGMRIGDFIHLVLPYPTFSELSKRVA
                     YTYYLPTLTKGWLRSIIGFLRRLG"
     gene            complement(4015..4803)
                     /locus_tag="VFY74_00790"
     CDS             complement(4015..4803)
                     /locus_tag="VFY74_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX5930278.1"
                     /translation="MKTASQSGDGVTYWLRRLWPLVALLGATACVLAMGWHRYLTITE
                     LAENREALRGYIEDHRLLSLAAFVGLYAAAVSLSLPGGAILTITGGFLFGWLLGGAAS
                     AVGATIGATILFLIARSTLGDLLAKRAGPLLSRFREGFQEDAFSYLLFLRLVPIFPFF
                     LVNLAPGLLGVSFATYVTTTILGIIPATFAFALAGDGLDSVIEAQQTMYQSCLAKMGS
                     GGQESCSYTLDPSALLTPELIAGLVALGLVALIPVAVKWFRRTA"
     gene            5052..5186
                     /gene="rpmH"
                     /locus_tag="VFY74_00795"
     CDS             5052..5186
                     /gene="rpmH"
                     /locus_tag="VFY74_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5930279.1"
                     /translation="MKRTYQPSRLVRKRRHGFRARMATKGGRKVLARRRAKGRKRLSA
                     "
     gene            5197..>5492
                     /gene="rnpA"
                     /locus_tag="VFY74_00800"
     CDS             5197..>5492
                     /gene="rnpA"
                     /locus_tag="VFY74_00800"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5930280.1"
                     /translation="MTAIGRLTTRREFLYVRDGTRFAAPSVVLQARQRATGAAEDPHL
                     ARFGFTASKTLGGAVVRNRARRRLKEAVRLAAPSHAREGYDYVLIAREGTVQRP"
BASE COUNT         1030 a         1750 c         1802 g          910 t
ORIGIN      
        1 tgaacggggg cggccgaccc ttagcgattg tgcgcgcgat ccgtctcgca tgctcattcg
       61 tcggcgaaga ctttggctgt gctcggttca ccgctgctac ctccgcgaat cacacgatgt
      121 tagctcgggg ccacttcccg gtcttgtcaa agatcccaaa gccgccacga cgatcgacgg
      181 aattgtgacg gccagcacat cgctcgggac tccacacctc gaaaatcgcg atgacgcctg
      241 tgacctcaaa gagggccgcc atgaacacca agaccgctct gatctccgcc atgatcgaaa
      301 tccgcggtcc gcgcgacggc gaattcaccg tcaagtttac cgctgcagac ggccgctcgc
      361 tcgcaattca tgtgccggta caacaaggat ctgtgcttcg cgatatccag gaaagcatgc
      421 cctacggtat tgccgtgcac gacttggtcg cggtcgaggt agaagccgag tgaccgccat
      481 gagccggcat gcgatcttgg ccccccgcgt tgaacggtac agccagaact ccagaggccg
      541 cagccgtgga ctgcaacgaa cttgcgcgga atctgctgct gcgcaaccct cgtcgtcgcc
      601 gtttcgagcg cgaaaacggc ggctgggccg gcgcctcacg ccggcgaagc ttgccgaagc
      661 cgcgagagcg tctttccagc aggctagaac gatcgccagc gaatggcgcg cccgaagaga
      721 ttcgaactcc tgacccccag attcgtagtc tggtgctcta tccagctgag ctacgggcgc
      781 gtctgacctc cagaagggga acctgcaaaa ggcgtgcctt taacaggcgt gccttccctc
      841 gaaggcgagc ttgagcccaa gcgcggatcg cgcaaaggaa gcgagcccta cctaatcggt
      901 cgcccgcgca aaggcaaggc tgggcgggtc ctcggcgggg cgatccagcg cgggggtcca
      961 ttccatgcct ccgtgcctgg aagcgtgacg gaatggatgc ccgggtcaag ccagggcatg
     1021 acgaagtaag aggcgtctgg cccttgcgcc cgatcaggcg ctgaggcgcc gcgctttctt
     1081 caactccacc accatatcgg ggattgtgag gcggaaggtg gcgccgcctt cgttctcgac
     1141 gagcgcgatc tggccgccat gggcctggac gagctcgtgc gcgatggcga gaccgagccc
     1201 ggtgccgcct ttgcgcacgc ttccctgaaa cgccgcgaag agatgcgcgc gggccttgtc
     1261 cggcacgccg ggtcccgtgt cggccacctc gatcacggcg accgcgccct cgcggcgccc
     1321 cgagacgacg atctctccct ccatcccggc ctcgctctcc agagcctgcg cggcattgcg
     1381 acagagattg ctcaagacgc ggtagagctg gtcgcggtcg gcatcgacct cgagcatcgc
     1441 ctcgacctcg acgctccagc cgatcctttg ccgcgggagc ccgagactgt cgccgatctc
     1501 tgtcacaagc ggccgaagcc tgaagcgctt ccgccgcggc ggcggctcct ggacctggcc
     1561 gtagttcagc gtctgcgcgc acagccggat ggcgcgatcg agcgacgcga tcagcttcgg
     1621 cacgaggcgc tgcaccgtcg ggtccttgac cgtgatcagg cggtcggaga ggagctgggc
     1681 gctcgacagc atgttgcgga gatcgtgact gatcttgctc accgcgagcc caagcgccgc
     1741 gagccgcgat ctctgcgaga gcgtctcggc gagctctttc tgcatggccg agagttcccg
     1801 ctcggcggtg ccgatctcgt cgcgccgggc ggaaggcgcg atgaccctgg tcggatcctc
     1861 ggggcgttgc gagaagcgga cgatgttcca ggtgagcttc gtcatgggtt tgatcaggag
     1921 ggcatcgagc gtgagataga cgagcgtggc ggtgatgatg gagatgagaa ttgacagccc
     1981 gagaatgtcg aggccgtaac ggatcatcgc cgctttcagc ggcgcttcgc ccatgacgac
     2041 ttcgatcgac tcgccttcgt ggaaaccggg atcgccgatc acgcggatga ccctgtcttc
     2101 gggcgcgaca tagaccatga gcgcgtccgc gatcagcttg agccaagagg cgtcgcgcag
     2161 gtcgtaagcc gcatcgatct cggcgggcat gtccatctcg atgatcagcg tgcgcgaggc
     2221 gtcgcgcttg acggcaatgg ccttgacctg ggcccgctcg aggagctcgt cgcgcagcat
     2281 cgaggggagc tggccgccgg gggccgcctc ggctgcgagc gaggcgatct gcgccgccga
     2341 cagccgctcc atcagccatt gccgccggaa attggacacc gaaggcacga acacgagcac
     2401 ttcggcgagc atgacgaaca caatggtgag cagcagcagc ttggccgaca gtcccggcac
     2461 gaagcggaac atcggcggct tttcgggagt cgtgtctggg ttctcgtcct tcaccaaaat
     2521 cactcgaacg caagcgatcg cgggcgcctt gagcgccgca tctatcctaa acggcgcagg
     2581 aaaccaatga tgctacggag ccagcccttc gtcaaagttg gcaaataata agtataggcc
     2641 acgcgcttcg agagctcgga aaaggtggga tagggcagga ccagatggat gaagtcgccg
     2701 atcctcatgc ccttggccat ggcgacgacc caaagctgca gcaattcgcc ggcctccgcg
     2761 ccgacgatgg tgcagccgag gatggtgccg ttcggcctgg tgatgacctt gatgaacccc
     2821 tccgtggcgc gctcgcattg ggcccggtca ttgtcggtat agggcgcgcg gtagatgcgg
     2881 atgcggcgat ggcgcttgcg cgcctcggct tcggtcaatc cgatctgggc aagctcgggg
     2941 tcggtataga ccgcgcgcgg aatgaggccg gcgctggcgt cggggttaag ccggaacaga
     3001 gcgttcctga tcacgatgcc ggcctggtaa ttggcgagat gggtgaagcc cggtccgccg
     3061 atcacgtcgc caacggcata gacgcggcga ttggtggttt tgagatgatg gttcactttg
     3121 atgccgtggt cgtcataggc gatcccggcc cgctcgaggc tgagcgattt gacgttcggc
     3181 ttgcgtcccg tggcgacgag cagatgcgag cctttgagga cggtgggctc gccgttctcg
     3241 gtaaagtgca cggaaattcc ttgcgtcgtg cgctcgatgc gctccacccg cgtatcgggg
     3301 tgaagcatca cttgcgtgtc ggccagccgc ttgatgagaa cggcggcagc ttccgggtcc
     3361 tcgtgggcga gaaagctctc cacatcgaac acgctgacat ggcagccgag catgcgatgg
     3421 gcctgcgcaa gctcgagccc gaccggaccg ccgcccagca cgatgagatg ctggatccgc
     3481 tcgctcacgt cgaagatcgt ctcgttggtg aaatagggca cctcgtcgag accgggaatc
     3541 ggcggaatgg acggcatcga tccggtggcg acgacgaatc ggcgggcgcg caccaggacc
     3601 cgcccggctt ccagcgtgac attgtcgagg aagctcgcct gcgcctggat gacacggacg
     3661 ccgaggcccg tcatgcgttc gatcgagtcg ttgggggcaa tggcggcgat gacgccgtgc
     3721 acgtgatcgt ggacgccgcg atggtcgatc tgggcctcga tcacgtcgat gccgaaggtc
     3781 gaggcggtgc gcacggcctc cgcccgcttc gccgccgcaa gcagggcctt ggagggaacg
     3841 cagccgtaat tgagacagtc cccgcccatg cggccctttt cgatgagcac caccggcaca
     3901 ccgaactggg ccgcggccgc cgcgaccgac aaacctcctg agcccgcgcc aatcacgcag
     3961 aggtcgggct tcagtacctc ttccatactc gctggtcccc catcgccaaa cgcttcaagc
     4021 cgtccgcctg aaccacttga ccgccaccgg aatgagcgcc acgagcccga gcgccacgag
     4081 ccccgcgatc agttccgggg tcaacagcgc gctcggatcg agcgtatatg agcacgactc
     4141 ttggcctccc gatcccatct tggcaaggca ggactggtac atcgtttgct gggcctcgat
     4201 gacgctgtcg aggccgtcgc cggccagcgc aaaggcaaag gttgccggga taatgccgag
     4261 aatagtggtg gtgacatagg tagcgaaact cacgccaagc agtccagggg caaggttgac
     4321 gaggaaaaag ggaaagatcg gcacgaggcg caggaagagc agataggaaa aggcatcctc
     4381 ctggaatccc tcgcgaaagc gcgaaagcag aggccctgcc cgcttcgcca agaggtcgcc
     4441 gagcgtgctt cttgcgatca ggaagagaat tgtcgctcca atagtcgcgc cgaccgcgga
     4501 ggcggcgccc cccaagagcc aaccgaacag gaaaccgccg gtgatcgtca ggatcgcacc
     4561 cccagggaga gacagcgaca cggcggcggc gtaaaggccg acgaaggcgg caaggctgag
     4621 gaggcgatga tcctcgatat aaccgcgcag cgcctcgcgg ttctcggcaa gctcggtgat
     4681 ggtcaagtag cggtgccacc ccatcgccaa cacgcaagcc gtcgcgccga gaagggctac
     4741 cagcggccaa agacgcctga gccagtacgt cacgccgtct ccgctttgcg acgccgtctt
     4801 cacttgatcg gcactccttt ttactggcag acctagatgg accatcttcg gcggatgcgg
     4861 aacagttctt cgcgctattg tgatgccacg tcgcgggcgc gcgtaagcct ggcgcgatcc
     4921 ggcgagcccg ttccgcccac aacggcgcgc caggattgac ttcggcggct tccccaccct
     4981 ataagccgcg gggtcgcgga ccaagccgag gcggtctatc gaccctcaat cccgagcaag
     5041 gagcccctcc cgtgaaacgc acctatcaac catcgcggct cgtccgcaag cggcgccacg
     5101 gctttcgcgc ccgcatggcg acgaaaggcg ggcggaaggt gcttgcgcgg cgcagagcca
     5161 aggggcgcaa gcgcctctcg gcttgacgcc tcaccggtga cggcgatcgg acgtctcacg
     5221 acccgcagag agttcctgta tgtacgggac ggcacgcgct tcgcggcgcc ctcggtcgtg
     5281 ctgcaggcac ggcagcgcgc gacgggcgca gccgaggatc cacatctcgc ccgcttcggc
     5341 ttcacggcaa gcaagacctt gggcggggct gtcgttcgca accgggcgcg gcgacgactg
     5401 aaagaggcgg tgcggctcgc cgcaccgtcg catgccaggg agggctatga ttatgtcttg
     5461 atcgcgcggg agggcacggt acaacggccc tt
//
LOCUS       DASUQP010000018        19960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_7846,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000018 DASUQP010000000
VERSION     DASUQP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 19960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19960
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7846"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(641..1375)
                     /gene="recO"
                     /locus_tag="VFY74_00805"
     CDS             complement(641..1375)
                     /gene="recO"
                     /locus_tag="VFY74_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5930281.1"
                     /translation="MDWSDEGIFLGAKPLGEANAVAELFTLGHGRHLGLVRGGRSRRM
                     RPLLQPGNRLRVTWRARLSEHLGGFNLELIEAHAARVLDDASALAAIGSLSGLARLLP
                     ERDPHPALYAAALGVIRAFDDPKLWPALLVRWELQLLQELGFGLDLSECAATGAETEL
                     AYVSPRSGRAVSRGAGQPYDGKLLKLPAFLREDDAPADEGDLLAGFALTGYFLERDVL
                     SPHGLVMPQARERLIGLLTRRFKIPA"
     gene            complement(1395..2324)
                     /gene="era"
                     /locus_tag="VFY74_00810"
     CDS             complement(1395..2324)
                     /gene="era"
                     /locus_tag="VFY74_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5930282.1"
                     /translation="MASEGAKSAESVSTRCGFVALIGAPNSGKSTLANALVGAKVSIV
                     THKAQTTRGPVRGIAPIGSSQVILVDTPGIFQPKRRLDRAMAQAAWERAGDADVVAVV
                     IDAERGLDQALEPLLSHLPEVKKPRIAILNKIDLVPKPKLLSLAADLSARGGFDRVFM
                     ISALTGDGIADLKTYLAEAVPPGPWLFPADQLTDQSLRQAAAEITREKLFLRLHEELP
                     YTLTVETEDWKERPDGSIRVEQTIYVERESQRKIVLGAKGRTIKEIGSSARAEIGAMV
                     DAPVHLFLFVKVRERWSEDPERYREMGLDYPKR"
     gene            complement(2317..3030)
                     /gene="rnc"
                     /locus_tag="VFY74_00815"
     CDS             complement(2317..3030)
                     /gene="rnc"
                     /locus_tag="VFY74_00815"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5930283.1"
                     /translation="MSRPRKSALKDLAARLGHDFSDPTLLELALTHSSARTGARLDDN
                     NERLEFLGDRVLGLAVAELLADSFPQAREGELARWFNQLVRAETCADIAQAWDLGAFI
                     LMSGGEAGSGGRRKKTILANTCEAVLGAVFADGGYEAAKRVVRAFWQPQLGTIDSAAP
                     DAKSVLQEWAQGRRLPLPRYLEVAREGPDHAPRFTAEVQIEGVAPERGQGANKREAEQ
                     AAALAVLLREGVWQAPQDG"
     gene            complement(3033..3788)
                     /gene="lepB"
                     /locus_tag="VFY74_00820"
     CDS             complement(3033..3788)
                     /gene="lepB"
                     /locus_tag="VFY74_00820"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5930284.1"
                     /translation="MSVEAEKREAKSGAKDTIRVIIHALILALLVRIFLFQPFNIPSG
                     SMIPTLLIGDYLFVSKYSYGYSRYSFPFGLDLFSGRVWAKEPARGDVVVFKLPRDNET
                     DYIKRVIGLPGDEIQMIHGVLNINGKPVPKVRIDDYVVDEPSGRERHLARFKETLPNG
                     VSYPVLDVVQDGFGDNTEAYKVPEAHFFVMGDNRDNSTDSRFLSEVGFVPFENLVGKA
                     QIIFFSMDEDSRFLEFWDWPTSIRWSRILQAVH"
     gene            complement(3882..4292)
                     /gene="acpS"
                     /locus_tag="VFY74_00825"
     CDS             complement(3882..4292)
                     /gene="acpS"
                     /locus_tag="VFY74_00825"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX5930285.1"
                     /translation="MIIGIGNDMIDIRRVERTIERYGERFLERVFTPVERMKSDGRAA
                     RAASYAKRFAAKEACAKALGTGFRQGVFWRDMGVVNLPSGRPTLELTGGAARALARLK
                     PEGHEMRIDLSITDDFPTAQAIVIISAVKVARRD"
     gene            complement(4289..5110)
                     /locus_tag="VFY74_00830"
     CDS             complement(4289..5110)
                     /locus_tag="VFY74_00830"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX5930286.1"
                     /translation="MTANRARPARGPSVAPIRLGINIDHIATLRNARGGRHPDPLRAA
                     KLAVEAGADNITAHLREDRRHIRDDDIARLAAANLAPLNLEMALTREMLAIALATRPH
                     ACCLVPERRQEVTTEGGLDAASQVSTLAPFVAELRRGGIQVTLFIDPDPAQIEAARKV
                     GADAVEFHTGTYCTAASEGGPARDSELARLVTGARDAARAGLEVHAGHGLDYDNVVPV
                     ASIPEIVELNIGHYLVGEAIFVGLAKSISRMRNLMDDAREALRHNVTEAKVGGTQ"
     gene            complement(5107..5667)
                     /locus_tag="VFY74_00835"
     CDS             complement(5107..5667)
                     /locus_tag="VFY74_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX5930287.1"
                     /translation="MLFKRREAESFGERMRVHLWPRRSWSRSSRYVVYRLRRLSDTPH
                     AVALGFAAGVFSALTPFIGTHLVFAMLLAWVLGGSVVAAVLGTFFGNPLTYPFIWYAT
                     YEVGSFMLGGKTHDHEIDLSGGIFQSSLEQLWPILKPMALGSLPLGLALAALSYVLVK
                     PMVEAYKNRRRRELELRSAREAMGAR"
     gene            complement(5753..7981)
                     /locus_tag="VFY74_00840"
     CDS             complement(5753..7981)
                     /locus_tag="VFY74_00840"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX5930288.1"
                     /translation="MTRQYELVERVLSYQPTADEALLNRAYVYAMKAHGNQKRASGAP
                     FFSHPLEVAAILAEMRLDDATIATALLHDTIEDTDTTRSEIDALFGREIGTLVDGLTK
                     IKKLDLVTKKAEQAENFRKLLLAISSDIRVLLVKLADRLHNMRTLGAMKPDKRKIVAE
                     ETMEIYAPLAGRMGMQWLREELEDHAFKALNPAAYATVVERLAILRERNHGLINEIAA
                     ALTAKLKEAKVAGFVAGREKKPHAIWSKMERKQISLEQLSDIYAFRIVVPEVEDAYRA
                     LAVVHTTWHTVPGRFKDYISNPKQNDYQSIHTTIVGPRHQRVELQLRTERMHSVAEYG
                     VAAHALYKDTNGPQGPASNGVPSQDSNAYRWLRHLVSMLLDGDNPEEFLEHTKLELFQ
                     DQVFCFTPKGSLIALPRGANPIDFAYAVHTGIGNTCVGAKINGRPMPLGTELRNGDEV
                     EIVCSKAQLPPSAWQSLAVTGKARSAIRRATRDAVRAQYGKLGREILVRAFQRRKKTF
                     SEASLTAALGRLSQKTAEDVMAAVGRGELASADVVRAVFPEETPQEAPKRRRKVKSSD
                     EGWFGLRKVMGLKFRWPGSGEKAREEGPSSIPIRGMRGDLPVSFAPGGAVPGDRIVGI
                     LTPGQGITIYPIHAEALKAFDEEPERWIDVTWDIDEDNPQRFPARISVTVLNEPGSLA
                     QVATVIGEANGNIDNIKMTNSADYTEMRIDLEVWDLKHLNDIIAGLKAKDMVSRAVRT
                     GA"
     gene            complement(8081..8491)
                     /gene="rpoZ"
                     /locus_tag="VFY74_00845"
     CDS             complement(8081..8491)
                     /gene="rpoZ"
                     /locus_tag="VFY74_00845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5930289.1"
                     /translation="MARVTVEDCIDKVPNRFELVLLASHRARTISAGAQIAVQRDNDK
                     NPVVALREIAEKKIASDDLREDFIHSMQKYVEVDEPEAEAVPMLSTDGRVPILGPDDQ
                     STDTAIDKMTEEELLRRMASQAPSDGSNAGPRSR"
     gene            complement(8559..9104)
                     /gene="folK"
                     /locus_tag="VFY74_00850"
     CDS             complement(8559..9104)
                     /gene="folK"
                     /locus_tag="VFY74_00850"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5930290.1"
                     /translation="MQTRSKVVIGLGSNHEGVWGTPAETIRRALTEIARAKIGVAAVS
                     PLYVTAAVGSARQPPYVNAVALLDTPLSPEALLRVLKAIERRSGRRGGRPWGPRTLDI
                     DILDYGGQVRLWRKGRAAVEGAGARPLTLPHPLAHRRPFVLRPLLDIAPDWRHPALKQ
                     SGRTLWRRASQRRQGAVLRRL"
     gene            9213..9827
                     /locus_tag="VFY74_00855"
     CDS             9213..9827
                     /locus_tag="VFY74_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5930291.1"
                     /translation="MNFYPNERIALFIDGANLYATAKSLAFDIDYKRLLALFRSKGQL
                     VRALYYTALVEDQEYSSIRPLIDWLDYNGYTMVTKPTKEFIDAAGRRKIKGNMDIELA
                     VDAMELADHLDHIVLFSGDGDFRSLVEALQHKGKRVSVVSTLATTPPMVADELRRQAD
                     QFIDLANLQAEIGRDPSERAPRGDRRRGEPSPLDELESEDLIET"
     gene            9872..10537
                     /locus_tag="VFY74_00860"
     CDS             9872..10537
                     /locus_tag="VFY74_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5930292.1"
                     /translation="MPSLDARPPAGPEPAKDCPLCPRLVAFRDENRRAHAEWYNGAVP
                     SFGTDRARLLIVGLAPGLKGANRTGRPFFGDYAGNLLYPTLLKAGLARGRHDQAAPEA
                     LELVDCMITNAVRCVPPQNKPKPEEVARCRAFLLARIEALPNLASLLAIGRIAHDAIL
                     DALKLKRGANPFAHGARHELPGGLLLFDSYHCSRQNTNTGRLTTAMFENVVAEAAASL
                     QRA"
     gene            10627..11028
                     /locus_tag="VFY74_00865"
     CDS             10627..11028
                     /locus_tag="VFY74_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEX5930293.1"
                     /translation="MAGLEISPAKVGFVIVKSREIAAKVAAWDDGSTSDHDAESILES
                     FSDDATQDELMGFIRDLNEDEQASLVALAWIGRGSFGPEELDEALATARAERTNRTDE
                     YLLGMPLLPDYLEEALDRLGYSVEDAEDDAL"
     gene            complement(11025..11579)
                     /locus_tag="VFY74_00870"
     CDS             complement(11025..11579)
                     /locus_tag="VFY74_00870"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5930294.1"
                     /translation="MTRLPPFPQAVDDGGGDHLTPGMRLPAIALPATDGGTIALAAIE
                     GRCLVIVYPWTGRSGLPNPPDWDSIPGAHGSTPELEGFRDRFADFAALGLRLFGLSRQ
                     ATVYQRELVMRLALPFPLLSDADGAFGAALCLPAFATGGTTYLKRLTLLIADGRIERV
                     FYPVIDPAAHAAEVLRMLEKRQER"
     gene            complement(11585..12055)
                     /gene="smpB"
                     /locus_tag="VFY74_00875"
     CDS             complement(11585..12055)
                     /gene="smpB"
                     /locus_tag="VFY74_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5930295.1"
                     /translation="MAPKRESGKLVAENRKARFNYDIEEKLEAGIALMGSEVKSLRAG
                     RANIAESYASDEGGELYLINAHIADYAGAARDGHAPTRPRKLLMHSREIARLLGAIQR
                     QGMTLVPLKLYFNARGIAKVELGLAKGKKLHDKRETEKKRDWERQKGRLMREKG"
     gene            complement(12074..12958)
                     /gene="dapA"
                     /locus_tag="VFY74_00880"
     CDS             complement(12074..12958)
                     /gene="dapA"
                     /locus_tag="VFY74_00880"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5930296.1"
                     /translation="MPPFKGSITALITPFKDGKVDARALQKLVEWQIDQGTHGLVPCG
                     TTGESPTLTHDEHRRVVELCVEAAAGRVPVIAGTGSNATAEAIELTHHAQSAGADGVL
                     VVTPYYNKPTQEGLYLHFKAINDCADVPIIIYNIPGRSVIDMSVETMARLFKLKNIVG
                     LKDATANMSRASLQRAALGTDFIQLSGEDATALGFMAHGGQGCISVTANVAPALCAEF
                     QLACLAGNFRLALQLQDRLMPLHDALFVESNPGPVKYAASRLGLCSADTRLPLAPLAP
                     ASRKKVDDALAAVGLVLV"
     gene            13429..15735
                     /locus_tag="VFY74_00885"
     CDS             13429..15735
                     /locus_tag="VFY74_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5930297.1"
                     /translation="MRASTAKPVKAKAPLKAKARVKPKAPIKKEAIAPSLPDRNPLRA
                     AATAELPAAVQETAEASPKPAPQIVTDMPRPDANPNRTGILEALRGTLTPAIAPPAPA
                     LDYASILRPILSYTLSSSDEINVHEVLRSGPAAAARIKDEAARDFALWYKYRNSPATG
                     NAEGIEQFRLSHPEWPGQDELREKAETALLIAGADTEKVKAFFSASPPQTGAGKAALA
                     SVYAKENNEAGARDLVVSAWRDHQLNAAVEKKILDRYGNWLTPEHHRSRIDRLLYPDE
                     KGAIEAALRVSKLLPPDEQKKVAARIAVIQRGGNAGKLLDALPSNAIEADVGLRFNRI
                     QWLRRTKNKERREQAWKMLLDAPSEPNILLDLNNWWTERRINCRGALNDGHPRVAYDI
                     AAKHGLVSGDSYIEAEFLAGWIALRFLNDPNNALKHFLSLRRVATSSKSIALGEYWLG
                     RTALALGDRGSAMVHFYRAAKYPQYFYGQLGRQSLDAKPAQLQITRTPVPSEADIQSF
                     LSRGAVRAIGIARATGFDGATPQFLLALSRKLTNPAEVVLVAELAKLMGDPQIALRLS
                     KIAFNRDLPLGDYALPIGGIPEFRSLLTDRVDPALVHALSRQESEFNAAAKSPVGASG
                     LMQLMPATARMVAKQYKVNYSANQLTNAAYNTQLGEAFLRGVIDDYSGSYIMALAAYN
                     AGGGRVAEWVKQFGDPRNPNVDPVDWIERIPFTETRQYVIKIMETLQLYRSRLAGPKQ
                     ALQLVQDLNRGRKIPHAKAIAGVQAKSE"
     gene            15779..16750
                     /locus_tag="VFY74_00890"
     CDS             15779..16750
                     /locus_tag="VFY74_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5930298.1"
                     /translation="MTSLLNTEATARAMPGRSVDTLPRSIPDMPELDGELWRDIYYAS
                     FDDLRLYVRHYPAPDAKLRPVICLPGLTRNGRDFQTLAAHLSKHPERPRNVYCFDYRG
                     RGRSQYDRRWRNYTPYIELNDILDFLTVHGLHKVGIVGTSRGGIIAMLMAALRPTAMG
                     PVVLNDIGPVIETRGLARISGYVGRMPVPKTWPDAVMLLREMNERAFPDFDDTQWEEI
                     ARDVFDERKGRPARAYDRKLARALGTIDLSRPVPDLWPQFIALGQVPVFVLRGANSDV
                     LSAETLEAMIARHPNLRAMTVPDQGHAPVLKEPETAETIGAFFAAND"
     gene            complement(16813..17844)
                     /locus_tag="VFY74_00895"
     CDS             complement(16813..17844)
                     /locus_tag="VFY74_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930299.1"
                     /translation="MRSHSMLGFRPRAVLLCLVAISALAGILAARADFSTAARGKLGV
                     SAPGHVLVAEKSAPGARGYVWSATRIESSIRLRGSVPSDDVRRTVLGMVKAHFPDLEV
                     EDRLKVNKGAPPQDQWLGAVSFGLQQLSHLKRGSVRLLDVALKVDGEARSAADYVQVK
                     KALAGPLPTGLTVVAKGSIKPPVADPFIFVADLGANALSLSGSVPSEGARSEVRELSR
                     QLFRRPGLDDRLELASGAPKDWDEAVAAALRALSQLESGKIALSGLAVTIEGVAPDKG
                     TAVAISYQLRRDLPALFSTSESISWKEASLSHDLAAKALPRIKHIAGAAGLWRGAKLP
                     DLLPPFDKR"
     gene            complement(18036..18182)
                     /locus_tag="VFY74_00900"
     CDS             complement(18036..18182)
                     /locus_tag="VFY74_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX5930300.1"
                     /translation="MTIDLVLLNPLIALIAGILILLMPRLLNFVVAIYLIVIGILGLV
                     PHFQ"
     gene            18457..19170
                     /locus_tag="VFY74_00905"
     CDS             18457..19170
                     /locus_tag="VFY74_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5930301.1"
                     /translation="MIVEQPAFWLAVLQIIWIDILLSGDNAVVIALACRKLPPRQRIW
                     GVVLGTIVAITLRVVFAGIVTTLMQVPYLKLVGGVLLLWIAVKLLGPEENGKGNEVQA
                     VDNLWRAIRIVAIADVVMSLDNVIAIAAVAERFEAPHNYALLMFGLAVSIPLIMAGAA
                     IVMALLERFPVVVWAGAALLGWIAGEIIAKDPLFTANLDPGTAHTWEIVGASLGALFV
                     IAAGMLRRRWLRRSAPDQI"
     gene            complement(19669..19944)
                     /locus_tag="VFY74_00910"
     CDS             complement(19669..19944)
                     /locus_tag="VFY74_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930302.1"
                     /translation="MVVEVVVDVVVEVVLVVVVEVVVDVLVEVVLVVEVVLDVVVLDG
                     VVVGPDVVVVGPDVVVVSSSRRLSRGDACALREPQRPLRGVWSSLSA"
BASE COUNT         3583 a         6392 c         6429 g         3556 t
ORIGIN      
        1 tttttttatc cgatcgtgaa atggggctcg gacgtcgcca cgatggtccg tgtcgggatt
       61 ggggccgatc ttgggcgaga tggcgaggcg tgagggaact accggcgaat cggcagcgtt
      121 tcgcaaatct ccatgttgtg cttggcaaag cagtaaggcg cggctattct gttctctcta
      181 tcggggaatt gtgattgccg aggcaagttc gtgccggccg cgctcaggcc ctagcctgtg
      241 gaagagaaag gaccggtgaa cggaatggga aatggccttt tgaccgcccg ccccgacagc
      301 aaagcaaggg gtccgcgtaa gctgctcgcg aaggttgtgg cagccgcggc gattgccttg
      361 ttttgtgcat cagcgcggtc gggacgacca ttggaaccac cgttggcgta accagcctgg
      421 ccaccgccgt caatgtcgcg accagcatgc ccgcggaagc ggggtaccgc cgcggccgtc
      481 ggcgccgccg tcgtcgtcgt cgtcgccgtt ggcatcgcgg caagtggcac tggtactggt
      541 ggtggtggta agtcgcgttc caacgcttag aaaaagcagc gggccggtct tatgaccggc
      601 ccgtttttcg tacgcgtcgc gcgcgcttgc gcccaaggca tcatgctgga atcttgaatc
      661 ggcgcgtcag aagtccgatg aggcgttccc tcgcctgtgg catgacgagg ccgtggggcg
      721 agagcacgtc acgctcgagg aagtatcccg tgagcgcgaa gcccgcgagg agatcgcctt
      781 cgtccgcggg cgcgtcatct tcgcgaagga acgccggcag cttgagcagc ttgccatcgt
      841 aaggttggcc agccccgcgc gacacggcgc ggcccgaacg cggcgaaaca tatgcaagct
      901 cggtttcggc gccggtcgcg gcgcattcgc tgagatcgag gccgaatccc agctcctgca
      961 agagctgaag ctcccagcgc acgagcagcg ccggccatag ctttggatcg tcgaaggccc
     1021 tgataacgcc gagcgccgcg gcgtaaagcg ccgggtgcgg atcgcgctca ggcaggaggc
     1081 gggcgagacc cgaaagcgag ccgatagcgg cgagcgcgct cgcatcgtcg agcacgcgcg
     1141 cggcatgcgc ttcgatcagc tcgagattga agccgccgag atgttcggag agccgcgccc
     1201 gccaggtgac gcgcaggcga ttgccgggct gaagcagagg ccgcatgcgg cgcgagcgtc
     1261 ctccccgcac gagcccgaga tggcgcccat ggccgagcgt gaacagctcg gcgacggcgt
     1321 tggcttcgcc aagcggtttc gcgccgagaa aaattccttc gtcgctccag tccatgcttc
     1381 gctcaagctt ggctctaacg cttcggatag tcgaggccca tctcgcgata gcgttcggga
     1441 tcctcggacc accgctcccg caccttgacg aagaggaaga gatggacggg cgcgtcgacc
     1501 atggcgccga tctcggcgcg agcgctggag ccgatctcct tgatggtgcg gcctttggcg
     1561 ccaagcacga tcttgcgctg gctttcgcgc tcgacgtaga tggtctgctc gaccctgatg
     1621 ctgccgtcgg ggcgttcctt ccagtcttcg gtctcgaccg tcagcgtgta gggcagctcc
     1681 tcgtgcagcc gcaggaacag cttttcgcgg gtgatctcgg ccgcggcctg ccgcagcgat
     1741 tgatcggtga gctggtccgc ggggaagagc caaggccccg gcggcacggc ttctgcgaga
     1801 tacgtcttga ggtcggcgat gccgtcgccc gtaagcgccg agatcatgaa cacccggtcg
     1861 aacccgcctc gcgccgaaag atcggcggca aggcttaaga gcttgggctt gggcacgagg
     1921 tcgatcttgt tgaggatggc gatacggggc ttcttcacct cgggcaaatg cgacaagagc
     1981 ggctcgagcg cctgatcgag accgcgttcg gcgtcgatga cgacggccac gacatcggcg
     2041 tcccctgccc gctcccaggc ggcttgcgcc atggcgcggt cgagccgccg cttgggctga
     2101 aaaatccccg gcgtatcgac gaggatgacc tgggaggagc cgatcggcgc gatgccgcgc
     2161 accggcccgc gcgtcgtctg cgccttgtgc gtgacgatcg agaccttggc gccgacgaga
     2221 gcgttggcca gtgtcgattt gccgctattc ggcgccccga tcagcgccac gaagccgcag
     2281 cgcgtggaaa cgctctcggc cgactttgcg ccttcgctag ccatcttggg gcgcctgcca
     2341 gaccccttcg cgcaagagca cggcaagcgc tgccgcctgc tcggcttcgc gcttgttcgc
     2401 cccctgcccg cgctcgggcg ccacgccttc gatttggact tcggcggtga agcggggcgc
     2461 gtggtcgggg ccttcgcggg cgacctccag atagcgcggg agcgggaggc gccggccttg
     2521 cgcccattcc tgaagcacgg acttcgcgtc gggcgcggcg gagtcgatcg tcccgagctg
     2581 cggctgccag aacgcccgaa ccacgcgctt tgccgcctcg tagccgccat cggcgaacac
     2641 ggcgccgagc acggcctcgc aggtgtttgc gaggattgtc ttcttgcggc gtccgccgct
     2701 cccagcctcg ccgccgctca tcaggatgaa cgcgccgagg tcccaggctt gcgcgatgtc
     2761 ggcgcaggtc tcggccctga cgagctgatt gaaccagcgc gcgagctcgc cttcgcgcgc
     2821 ctgggggaag gaatccgcca ggagctcggc aacggcgaga ccgagcacgc ggtcgccgag
     2881 gaattccaga cgctcattgt tgtcgtcgag tctcgcgccc gtgcgcgcgc tcgagtgggt
     2941 caaggcaagc tcgagcaagg tcggatcgga gaagtcgtgc ccaagccggg ccgcgaggtc
     3001 cttgagcgcc gatttgcgcg gccgtgacaa gctcagtgta ccgcctgaag aatgcgcgac
     3061 cagcggatgc tggtcggcca atcccaaaac tcgagaaatc gagagtcctc gtccattgag
     3121 aaaaagatga tctgcgcctt gcccacgaga ttctcgaacg gcacgaaccc gacctccgag
     3181 agaaagcggc tgtcggtcga gttgtcgcga ttgtcgccca tcacgaagaa gtgtgcttcg
     3241 ggcactttgt aagcctcggt gttgtcgccg aagccatctt gaaccacgtc gaggacggga
     3301 tagctcacgc cgttcggcaa cgtctccttg aatcgcgcga ggtgtcgctc gcgccccgaa
     3361 ggctcgtcca cgacataatc gtcgatgcgg acttttggca ccggcttgcc gttgatgttg
     3421 agcacgccgt ggatcatctg gatctcgtcg ccgggaaggc cgatcacgcg cttgatatag
     3481 tcggtctcgt tgtcgcgcgg cagcttgaat acgacgacgt cgccacgcgc aggttccttg
     3541 gcccagaccc ggccggagaa gagatccaat ccgaacggga aggagtagcg gctgtagccg
     3601 tagctgtatt tcgaaacgaa cagatagtcg ccgatcagaa gcgtcgggat catcgagccg
     3661 gacgggatat tgaaaggttg aaagaggaaa atgcgcacga gaagcgcgag gatcagtgca
     3721 tgaataatga cgcgaatggt gtccttcgcg ccactctttg cttccctctt ctccgcttcg
     3781 acgctcatgc ggaagcactc cttcggtctt acgtgggaat tccccgcccg cttcagttcg
     3841 gccgccccct atagcccctc caataggggg aggcaaaccg ctcagtcgcg gcgggcgacc
     3901 tttacggccg agatgatgac gatcgcctgg gccgtgggga aatcgtcggt gatggacagg
     3961 tcgatgcgca tttcgtggcc ttctggcttc agccgggcga gtgcccgggc cgcgcctccg
     4021 gtcaactcca atgtggggcg ccctgacggc aaattgacga cgcccatgtc gcgccagaac
     4081 accccctggc gaaatccggt gccgagcgcc ttggcgcaag cctccttggc ggcgaagcgc
     4141 ttggcgtaag aggcggcgcg ggccgccctg ccatcggact tcatccgttc gacgggggtg
     4201 aaaacgcgct cgagaaaacg ctcgccgtag cgctcgatgg tgcgctcgac gcgtctgatg
     4261 tcgatcatgt cgttgccgat gccgatgatc attgggttcc gccgaccttg gcctcggtga
     4321 cgttatgccg caaggcctcc cgcgcgtcgt ccatcaggtt tcgcatgcgc gaaatgctct
     4381 tggcgagccc aacgaagatc gcttcaccga cgaggtagtg accgatattg agttcgacaa
     4441 tttcgggaat cgaggcgacg ggaacgacgt tgtcatagtc gagaccatga cccgcatgca
     4501 cttcgaggcc ggcccgcgcg gcgtctcgcg cgccggtgac gagacgcgca agctcgctgt
     4561 cgcgcgcggg acctccctcg gacgccgccg tgcaataggt gccggtatgg aactcgacgg
     4621 cgtcggctcc cactttgcgc gcggcttcga tctgcgcggg atcgggatcg atgaagagcg
     4681 tgacctgaat gccgccgcgc cggagttcgg cgacaaacgg ggcgagggtc gagacttggc
     4741 tcgcagcatc gagcccgcct tcggtggtca cctcctgtcg ccgttccggc acgagacaac
     4801 aggcatgcgg gcgcgtggcg agcgcgatcg ccagcatctc ccgcgtgagc gccatttcaa
     4861 gattgagcgg cgcgaggttg gccgcggcga gccttgcgat atcgtcgtcg cggatgtggc
     4921 ggcgatcttc gcgcaaatgt gccgtgatgt tgtcggcacc ggcctcaacc gcgagtttcg
     4981 ccgcgcgtaa aggatcagga tgacgcccgc cccgcgcgtt ccgcaacgtc gcgatatggt
     5041 cgatgttgat gccgagacgg atgggagcaa cgcttgggcc gcgcgccggt ctcgctctgt
     5101 tggcggtcat cgggctccca tggcttcccg ggcggagcgc agttcgagct cgcggcggcg
     5161 gcgattcttg taggcctcga ccatcggctt gacgagcaca tagctgaggg ctgccaaggc
     5221 gagcccgagc ggcaaggagc cgagcgccat cggtttcagg atcggccaca actgctcgag
     5281 cgaggactgg aagatgccgc cggagagatc gatctcgtgg tcgtgggtct tgccgcccag
     5341 catgaaactg ccgacctcgt aggtggcgta ccagatgaag ggataggtca acggattgcc
     5401 gaagaaagtt ccgagcacgg ccgcgacaac gctgccgccg aggacccagg cgagaagcat
     5461 ggcaaagacg agatgcgtgc cgatgaacgg cgtcagcgcc gagaacacgc cggcggcgaa
     5521 gccgagcgcc acggcatgag gggtgtcgct caatcgcctc aggcggtaga cgacgtagcg
     5581 gctggagcgg ctccagctcc gccggggcca caaatgcacc cgcatccgtt cgccgaagct
     5641 ttccgcctcg cggcgcttga acagcatcac acctcactac gcacacgaag gtggcatcgg
     5701 gcgacaggcc catagcaata tttaggtaaa attcccttag ggcgccaggg cgctaggcgc
     5761 ccgtgcggac ggcccggctc accatgtcct tggccttcag cccggcgatg atgtcgttga
     5821 ggtgcttgag gtcccacact tcgagatcga tccgcatctc ggtgtagtcg gcgctgttgg
     5881 tcattttgat attgtcgata ttgccattgg cttcaccgat cacggtggcg acctgcgcga
     5941 ggctgccggg ctcgttcagc accgtcacgg agatcctcgc cgggaagcgc tgcggattgt
     6001 cttcgtcgat gtcccaggtc acgtcgatcc agcgctcggg ctcctcgtcg aaggctttca
     6061 acgcctcggc atggatcgga tagatagtga tgccctgacc cggggtcagg atgccgacga
     6121 tacggtcgcc gggaacggcg ccgccgggag cgaagctcac gggcaggtcg ccgcgcatgc
     6181 ccctgatcgg gatgctgctc ggtccttctt cgcgcgcctt ttcgccggac cccggccagc
     6241 gaaacttgag gcccatgact ttgcgcagtc cgaaccagcc ttcgtcgctg cttttgactt
     6301 tgcggcgccg cttcggagcc tcttgcggcg tttcctcggg gaacaccgcg cgaaccacgt
     6361 cggccgaggc gagctcgccc cggcctaccg cggccatcac gtcctcggcg gtcttttgcg
     6421 acaggcggcc gagggcggcg gtgagacttg cctcactgaa cgtcttcttg cggcgctgaa
     6481 aagccctgac caggatttcg cggccgagct tgccgtattg ggcgcggacg gcgtcacggg
     6541 tggcgcgccg gatggccgag cgcgccttgc cggtgacggc aagactctgc cacgccgaag
     6601 gaggaagctg agccttcgag cagacgatct cgacctcgtc gccgttcctg agctcggtcc
     6661 cgagcggcat gggacgtcca ttgatcttgg cgccgacgca ggtgttgccg ataccggtgt
     6721 gcacggcata ggcaaaatcg atggggttcg ctccccttgg gagcgcgatc aagcttcctt
     6781 tgggcgtgaa gcagaagacc tggtcctgga acagctcaag cttcgtgtgc tcgagaaact
     6841 cctccgggtt gtcgccgtcg agcagcatgc tgacgagatg acggagccag cgataagcgt
     6901 ttgaatcctg cgacggtacc ccgttgctcg cgggcccttg cgggccgttc gtatccttgt
     6961 agagcgcatg cgctgccacg ccatattcgg cgacggagtg catccgctcg gtgcggagct
     7021 gcaattccac gcgctgatgg cgcggcccga cgatggtggt gtggatcgac tggtagtcgt
     7081 tctgcttcgg gttcgagatg taatccttga agcggccggg cacggtatgc caggtcgtgt
     7141 gcacgacggc cagcgcgcgg taggcatcct cgacctcggg cacgacgatg cggaaggcat
     7201 agatgtcgga gagttgctcg agcgagattt gcttccgctc catcttgctc cagatggcgt
     7261 gaggcttctt ctcccggccg gcgacgaagc cggcgacctt cgcctctttc agcttggcgg
     7321 tcagcgccgc ggcgatttcg ttgatcaggc catggttgcg ctcgcgcagg atcgcgagcc
     7381 gctccaccac cgtggcgtaa gccgcgggat tgagcgcctt gaaggcgtgg tcttccagct
     7441 cttcgcgcag ccactgcatc cccatgcgcc cggccaaggg cgcatagatt tccatggtct
     7501 cttcggcaac gatcttgcgc ttgtcgggct tcatcgctcc gagcgtgcgc atgttgtgga
     7561 ggcggtcggc gagcttgacc aagagcacgc ggatatcgct cgaaatggcg agcaagagct
     7621 tgcggaagtt ctccgcctgc tccgccttct tggtgaccag gtcgagcttc ttgatcttgg
     7681 tgaggccgtc gacgagcgta ccgatctcgc ggccgaacag cgcgtcgatc tcgctgcggg
     7741 tggtgtcggt gtcctcgatg gtgtcgtgca ggagcgcggt ggcgatggtc gcgtcgtcga
     7801 gccgcatctc ggcaaggatc gccgcgacct cgagggggtg cgagaagaag ggcgcgcccg
     7861 aggcccgctt ctgattgccg tgcgccttca tggcgtagac ataggcgcgg ttcagcagcg
     7921 cctcgtccgc ggtcggttga tagctcaaga cccgctcgac cagctcgtat tggcgggtca
     7981 tcgcgcggac tttccgccgt cagcgaagtg aagccaaagc ggcgcttccc ttgcccgcgt
     8041 gacaaagcga caggcggagc acctcaatgc aagggcaaag tcagcgcgag cggggtcccg
     8101 cattggagcc atccgaaggc gcttggctcg ccatgcggcg cagcagctcc tcctcggtca
     8161 tcttgtcgat cgccgtgtct gtcgactgat cgtccgggcc gaggatcggc actctgccgt
     8221 cggtcgagag catcggcacg gcttcggcct caggctcatc cacctcgaca tatttctgca
     8281 tggagtggat gaagtcctcc cgcaaatcgt cggaagcaat tttcttctcg gcaatttcac
     8341 gcagcgccac gaccgggttc ttgtcgttat cgcgctgcac ggcgatctgg gcgccggcgg
     8401 aaattgtgcg ggcgcggtga ctggccaaga gcacgagctc gaagcgattc ggaaccttat
     8461 cgatgcaatc ctcgacggtg acgcgagcca tcaggcaatc cttgtctaga gaaggcgatt
     8521 tgttcctatt taggcgcttc ccgcaggcca ggcaagcgtt acagccgcct cagcactgcc
     8581 ccctgcctcc gctgcgacgc ccgccgccac aacgtgcgtc cgctttgctt gagcgcggga
     8641 tggcgccagt cgggtgcgat atcgagcagc gggcgaagga cgaaggggcg ccggtgggcg
     8701 agcggatgcg gcaaagtaag aggccgcgcg cccgcgcctt cgacagccgc ccgtcctttc
     8761 cgccaaagcc tgacctggcc gccgtaatcg agaatgtcga tgtcgagggt gcgggggccc
     8821 cagggcctgc cgccgcgccg gcccgaacgg cgctcgatcg ccttgagcac ccgaagcagt
     8881 gcctcgggcg agagcggcgt gtcgagcagc gccacggcat tcacataggg cggctgccgg
     8941 gcgctcccga ccgccgccgt cacgtaaagg ggagagactg ccgcgacacc gatcttggcc
     9001 cgcgcgattt cggtcaaggc gcggcgaatc gtctcggccg gcgttcccca cacgccttca
     9061 tgattggagc cgaggccgat cacgactttg ctacgcgtct gcaattgcgg agccacgccg
     9121 tcgtacctat gttgtgtcag tggccgtctc atcccgcatt gcgagaggcc gcttgctatc
     9181 aatcaagtca tattgcagtg aacggaagac aaatgaactt ctacccgaac gaacgaatcg
     9241 cactcttcat cgacggggcc aatctctacg caaccgccaa gtcgctcgcc ttcgacatcg
     9301 actataagcg gctgctcgcg ctgttccgct ccaagggtca gctcgtgcgc gcgctctact
     9361 acacggccct ggtggaagac caggagtatt cctcgatcag gcctttgatc gattggctcg
     9421 actacaacgg ctacaccatg gtgaccaagc cgaccaagga attcatcgac gcggctgggc
     9481 ggcgcaagat caagggcaac atggacatcg agcttgcagt cgacgccatg gagctagccg
     9541 atcacctcga ccatatcgtg ctgttctcgg gcgacggcga tttccgctcg ctcgtcgagg
     9601 cgcttcagca caagggcaaa agggtaagcg tcgtttccac tctcgccaca acgccgccga
     9661 tggtggccga cgagctgcgc cggcaagccg atcagttcat cgatctcgcc aatctgcaag
     9721 ccgaaatcgg ccgcgatccg tccgagcggg cgccgcgcgg cgatcgccgg cgcggtgagc
     9781 ctagtccgct cgatgagctc gagtcggaag atctgatcga gacctaatct cggtagtggc
     9841 gcgaggccgt gcgtgacgct aggcatgacg catgccatcc ctcgacgcga ggccgccagc
     9901 agggcctgag cccgccaaag attgcccact ctgtccgcgc ctcgtcgcct tccgcgatga
     9961 gaacaggcga gcgcatgctg agtggtacaa tggcgcggtt ccctccttcg gcaccgatcg
    10021 cgcgcggctt ctcatcgtcg ggctcgctcc cgggctcaaa ggcgcaaacc gcaccggccg
    10081 tccgttcttt ggcgactatg ccggcaacct cctctatccc accctgctca aggctgggtt
    10141 ggcgcgcggt cggcacgatc aggccgcgcc cgaggcgctt gagcttgtgg attgcatgat
    10201 caccaatgcg gtgcgctgtg tgccgccgca gaacaagccg aagcccgagg aggttgcacg
    10261 ttgccgcgcc ttccttctcg cccgcatcga agccctgccc aacctcgcct cgctccttgc
    10321 catcggccgc atcgctcatg acgccattct ggatgcgctc aagctgaagc ggggcgccaa
    10381 tccctttgcg catggagccc ggcacgagct tccgggaggg cttctgctgt tcgacagcta
    10441 tcattgctcg cgccagaaca cgaatactgg gcgcctgacg acggccatgt tcgaaaacgt
    10501 cgtcgccgag gccgcggcga gcttgcagag agcgtgagga agagcaagcg agcctccttg
    10561 cagccgcccg gcaagtcgtg catggtttaa gggcggcgcc ggggacacac agcagggatg
    10621 gaccccatgg cggggttgga aatttcgccg gccaaagtcg gctttgtgat cgtcaaatcg
    10681 cgtgagattg ccgccaaggt tgcggcctgg gacgatggct ccacgtccga tcacgacgcc
    10741 gagtcgatcc tcgaatcgtt ctccgacgac gcgacgcaag acgagctcat gggctttatc
    10801 cgcgacctca atgaggacga gcaggcgagt ctggtcgcgc tcgcctggat cggcagaggt
    10861 tcgttcggtc ccgaagagct cgacgaggcg ctcgccacgg cgcgggccga gcgcaccaat
    10921 cgcaccgacg agtatctcct cggcatgccc ctcctccccg actatctcga agaggcgctc
    10981 gaccgtctgg gctactcggt cgaggatgcc gaggacgacg cgctttagcg ctcctgcctt
    11041 ttctcaagca tgcgcaaaac ttcggcggcg tgagcggccg gatcgatcac gggatagaag
    11101 acccgctcga tgcgaccgtc cgcgatgaga agcgtgaggc gcttgaggta ggttgtcccg
    11161 ccggtggcga aggccgggag gcagagcgcc gcgccgaacg cgccgtcggc gtcgctcaga
    11221 agcggaaacg gcaaggcaag acgcatgacg agctcgcgtt gatagaccgt cgcctggcgg
    11281 ctcagtccga agagccgcaa cccaagcgcg gcgaaatcgg caaaccggtc gcgaaagcct
    11341 tcgagctccg gcgtcgagcc atgcgcgccg gggatggaat cccaatccgg cggattggga
    11401 aggccggagc gaccggtcca gggataaacg atgacgaggc atcgcccttc gatcgcggca
    11461 agggcaatcg tgccgccgtc tgtggccggc aacgcgatgg cgggcagcct catgccgggc
    11521 gtgaggtgat cgccgccacc gtcatcgacc gcctgaggaa acggcggaag gcgcgtcacc
    11581 ctccctaccc tttctcgcgc atcaagcggc ccttctgccg ctcccagtcc cgcttcttct
    11641 cggtctcgcg cttgtcatgc agcttcttgc ccttggcaag accgagctcg accttggcga
    11701 tgccccgcgc gttgaaatag agcttgagcg gcacgagcgt catgccctgg cgctgaatgg
    11761 cgccgagcag gcgggcgatc tcgcgcgaat gcatcagaag cttgcgcggt cgcgtgggcg
    11821 cgtggccgtc acgggcagcg ccggcatagt cggcgatatg agcgttgatg agataaagct
    11881 cgccgccctc gtcggaggca taggactccg cgatattggc cctgccggcg cggagcgact
    11941 tcacctccga acccatgagc gcgataccgg cctcgagctt ctcctcgatg tcgtaattga
    12001 agcgcgcctt ccggttctcg gcgacgagct tgccgctctc gcgcttcggc gccatgacgt
    12061 caccttgccg gcatcaaacg agcacgaggc cgaccgccgc cagagcgtcg tcgactttct
    12121 tccgcgacgc gggcgcgagc ggcgcgagcg gaagccgagt gtcggcgctg catagcccga
    12181 gcctgcttgc ggcgtacttc accgggccgg gattcgactc cacgaacagc gcgtcgtgca
    12241 gcggcatcag ccggtcctgc aactggaggg caagcctgaa attgccggcg aggcaggcga
    12301 gctggaattc ggcgcagagt gccggggcca cattggcggt cacggagatg cagccttgcc
    12361 cgccatgggc catgaagccg agcgcggtcg cgtcttcgcc tgagagctgg atgaaatcgg
    12421 tgccgagagc cgcgcgttgc aggcttgcgc gcgacatgtt ggcggtcgca tccttgaggc
    12481 cgacgatatt cttgagcttg aacagccgcg ccatggtctc gaccgacatg tcgatgacgg
    12541 agcggccggg gatgttgtag atgatgatgg gcacgtcggc gcagtcgttg atggccttga
    12601 aatgcagata gagcccttcc tgcgtcggct tgttgtaata cggcgtcacc acgaggacgc
    12661 catcggcgcc ggcgctttgg gcatgatggg tcagttcgat ggcctcggcg gtcgcgttcg
    12721 atccagtccc ggcgatcacg ggcacgcgtc ctgcggccgc ttccacgcaa agctcgacga
    12781 cgcgccgatg ctcgtcatgg gtgagcgtgg gcgattcgcc tgtcgtcccg cacggcacga
    12841 gaccgtgcgt gccctggtcg atctgccact cgacgagctt ttgcagggcg cgggcatcga
    12901 ccttgccatc cttgaacggg gtgatgaggg ccgtgatcga gcctttgaac ggcggcatgg
    12961 gcacgcgggt cactccttaa aggtctcgcg cttgatctcg gggcggaaag gacggcggac
    13021 aatagggtgc gcccaccgag gcggcaaggc gccggaagcc cttggaatgc gcccttgaca
    13081 tgcgcccgtc ccggcacagc gcgccggcct cgacgggcgg gcaaagtgta tgcgtttcaa
    13141 taacttggct tgattctctc agtcggttcg cagttcgctg gacgggcgtt tgagacattt
    13201 ttctgccgga ttctttcaga cattgttcaa ttcaggcaca ttacactgcc gtcttcgtcg
    13261 cggctccccg gatcggggga ctgtcctgac cgcgtaaaac aggatcgtaa ccggaaaccg
    13321 atcgacgaga tgaaacgcat tagagcgtac atcgtaccat tactcgtttc cgccgcggtt
    13381 ctcgctccgg ccttggccga ggcgacgacg cagcgttcgg ctaagtccgt gcgcgcgtcg
    13441 acagccaagc ctgtcaaggc gaaggcgccc ctgaaagcca aggcgcgggt caagcccaag
    13501 gcgccgatca agaaggaagc gattgcgccg tccctacccg accgcaatcc gctcagggcg
    13561 gccgcgaccg cggaacttcc ggctgcggtt caggaaacgg ccgaagcttc gcccaagcct
    13621 gcgccgcaaa tcgttaccga catgccgcgg ccggatgcca atcccaatcg gacgggaatc
    13681 ctggaggcgc tgagaggcac gctgacgccg gccatcgcgc cgcccgcgcc cgcgcttgac
    13741 tacgcatcga tcttgagacc gatcctgtcc tacacgctga gcagctccga cgagatcaat
    13801 gtccatgaag tgctgcgttc gggccccgcc gcggccgcgc gcatcaagga cgaggccgcc
    13861 cgcgactttg cgctctggta caagtaccga aacagcccgg cgaccggaaa tgccgaaggg
    13921 atcgagcagt tccggctttc gcatccggag tggccggggc aggacgaatt gcgggagaag
    13981 gcggagaccg ccctcctgat cgccggcgcc gataccgaga aggtcaaagc cttcttcagc
    14041 gcgtccccgc cgcaaacggg tgccggcaag gccgcgctcg cttcggtcta cgcgaaggag
    14101 aacaacgaag cgggcgcgcg cgatctcgtc gtttcggcct ggcgcgacca ccagctcaat
    14161 gcggcagtgg agaagaagat cctcgaccgc tacggcaact ggctgacgcc tgagcatcac
    14221 cggtcgcgca tcgaccggct gctttatccc gacgaaaaag gcgcgatcga agcggcgctc
    14281 cgggtgtcca agctgctccc gcccgacgag cagaagaagg tcgcggcccg tatcgccgtg
    14341 atccaacgcg gcggcaacgc cggaaagctt ctcgacgccc tgccgtccaa tgcgatcgag
    14401 gctgatgtcg gcctgcggtt caaccgcatc cagtggctgc ggcgcaccaa gaacaaggag
    14461 cggcgcgagc aagcctggaa gatgctcctc gatgccccca gcgagccgaa cattctgctc
    14521 gacctcaaca attggtggac cgaacggcgc atcaactgcc gcggcgcact caatgacggt
    14581 cacccccggg tcgcctatga catcgccgcc aagcacgggc tcgtatccgg cgacagctat
    14641 atcgaggctg aattcttggc cggctggatc gcgctccgtt tcctgaacga tcccaacaac
    14701 gctttgaagc atttcttgtc gttgcgccgc gtggcgacga gttcaaagag catcgcgctc
    14761 ggcgaatact ggcttggccg cacagctctt gccctcggcg atcggggctc ggcgatggtg
    14821 catttctacc gcgccgccaa atacccccaa tatttctacg gccaactcgg gcggcagtcg
    14881 ctcgacgcca agcccgccca gctccagatc acccgcacgc ccgtgccgag cgaggccgac
    14941 attcagagct tcctgtcccg gggcgccgtg cgggccatcg ggattgcgcg cgccacgggc
    15001 ttcgatggcg cgacaccgca atttttgctg gcgctgtcgc gcaaactgac caaccccgcc
    15061 gaagtcgtgc tggtcgccga actcgccaag ctcatgggcg acccgcagat cgcgctccgg
    15121 ctgtcgaaga tcgccttcaa ccgcgatctt cccctcgggg actatgctct gccgatcggg
    15181 ggcatacccg aattcaggag cctgctgacc gaccgggtcg acccggcgct ggtgcatgcc
    15241 ctgtcccgac aagaaagcga gttcaacgcc gccgccaaaa gcccggtggg cgcgagcggc
    15301 ctgatgcagc tcatgccggc cactgctcgc atggtggcca agcagtacaa ggtgaactac
    15361 tcggccaacc aacttaccaa cgctgcctac aacacccagc tcggtgaagc gttcttgcgc
    15421 ggcgtcatcg acgactatag cggctcctat atcatggcgc tcgccgccta taatgcggga
    15481 ggcggtcgcg ttgccgagtg ggtcaagcag ttcggcgacc cgcgcaaccc caacgtcgat
    15541 cccgtcgact ggatcgagcg catcccgttc accgagacgc ggcaatacgt gatcaagatc
    15601 atggagacgc tgcagcttta ccgctcgcgg ctggcgggac ccaagcaggc gttgcagctc
    15661 gttcaggatc tcaatcgcgg ccggaagatt ccccacgcca aggcaatcgc cggcgtgcag
    15721 gcgaaatcgg agtgaaacga gcaggctcgc gttttcgccg cttgtaaggc ttgccccggt
    15781 gacaagtttg ctgaacactg aagcgacggc gcgggcgatg cctggccgat cggtcgatac
    15841 gctcccaagg tccatccccg acatgcccga gcttgacggc gagctgtggc gagacatcta
    15901 ctatgcgtcg ttcgacgacc tcaggctcta tgtccgccac tacccggcgc cggacgcgaa
    15961 gttgcgcccc gtcatctgtc tcccgggact gacccgcaac ggcagggatt ttcaaacgct
    16021 tgcggcccac ctgtcgaagc atccggagcg gccgcgcaac gtctattgct tcgattatcg
    16081 cgggcgcgga cgttcgcaat acgatcgccg ctggcgcaac tatacgccct atatcgagct
    16141 caacgacatt ctcgatttct tgacggtgca cggcttgcac aaggtgggca tcgtcggcac
    16201 gtcgcgcggc ggcatcatcg ccatgctgat ggcggcgctc cgaccgacgg caatgggccc
    16261 ggtcgtcctc aacgatatcg ggcccgtcat cgaaacgcgc gggctggcac gcattagcgg
    16321 ctatgtcgga cgcatgcccg tgccgaagac atggccagac gccgtcatgc tgctgcgcga
    16381 gatgaacgag cgtgccttcc ccgacttcga cgacacgcaa tgggaggaga tcgccaggga
    16441 cgtgttcgac gagcgcaagg gccgcccagc ccgggcctac gaccgcaaac ttgcgcgagc
    16501 gctcggcacg atcgatctga gccgcccggt gcccgacttg tggccgcaat tcattgcgct
    16561 cgggcaggtg ccggttttcg tgctgcgggg ggccaattcc gacgtgctca gcgccgagac
    16621 gctcgaggcc atgatcgcgc ggcaccccaa tctccgtgcc atgacggtgc cggaccaggg
    16681 gcatgctccc gtgctcaagg agccggaaac ggccgagacg atcggcgcct tcttcgccgc
    16741 aaacgattga cgcttgcctt gcgggagttc agctcctcct cgcgtctcgc gctcaaggcg
    16801 agagacaggg cgttaccgct tgtcgaaggg cggaagcaga tccggcagct tggctccccg
    16861 ccacaggccg gcggccccgg cgatgtgctt gatgcgcgga agggccttgg ccgcgagatc
    16921 atgcgagagg gacgcttctt tccagctgat gctctcagag gtggagaaga gcgccggcag
    16981 atcgcgcctg agttggtagg aaatcgcgac cgccgtcccc ttgtccggcg ccacgccttc
    17041 aatcgtcacc gcgagccccg acaaggcgat cttccccgat tcgagctggc tcagcgcgcg
    17101 cagcgccgca gccaccgcct catcccaatc tttcggcgca ccggaggcga gttcgagccg
    17161 gtcgtcgagg ccgggccggc ggaacaattg tcgtgacagc tcccgcacct cgctccgcgc
    17221 accctcgctc ggcacgctcc ccgacaagct caacgcgttg gcgccaaggt cggcgacaaa
    17281 gatgaaggga tcggccaccg gaggcttgat gcttcccttg gcaacaacgg tgagccccgt
    17341 cggcagaggt ccggcaaggg ccttcttcac ttgaacgtag tcggcggcac tgcgcgcctc
    17401 gccgtcgact ttcagcgcga catcgagcag ccgcaccgag ccgcgcttca aatgcgacag
    17461 ctgctgaagc ccgaagctga cggcgccaag ccactgatcc tgcggcggcg cgcccttgtt
    17521 cactttgaga cgatcctcga cttcaagatc gggaaaatgc gccttgacca tgccgagaac
    17581 ggttcggcga acgtcgtcgg agggaaccga gccgcgaaga cggatgctcg attcgatgcg
    17641 cgtggccgac cagacatagc cccgggcgcc gggcgcggac ttttccgcga ccaggacatg
    17701 gcccggcgcc gacacgccga gcttccctcg cgcggcggtg ctgaaatcgg cgcgtgcggc
    17761 aagaatgccg gccagagccg agatggcgac aaggcagagc aacaccgctc tcggccggaa
    17821 ccccaacatg gaatgcgacc tcatgcgtcg taacgccttg caggaatcgg ttgcagcatc
    17881 gtgcagacga tgacctgttt ctccatgcgc gtgaatgacc gaactataag ccatccgcac
    17941 gaaaggttcc aagcaacact ttgcggtttc gcacgatgtt catcaggcga acccagcaaa
    18001 ccccctccct cgcgcctctc gcagcgccac gcttgctatt gaaaatgagg gacaaggcca
    18061 agaatgccga tcacgatgag atagatcgca acaacaaagt taaggaggcg cggcatgagc
    18121 aggatgagaa ttccggcgat caaggcaatc aacggattga ggagcacaag atcgatggtc
    18181 atggtttttc cccgtgagat gggccggacc gcctgattcg gccggttgaa cgaccgagca
    18241 tatgccatcg gctgcgactt gtcgtggcgg gaaacagtcc gtgaacattg aatcgcctcc
    18301 gggcccgatc ggtcttgatc cgccctcctt ctatcttgtt tgaaaaagca tatatcccgt
    18361 cttgtccttg ccgtttttcg cggcaaggga tagttgacca gcccgttgtt ggggcggcaa
    18421 tgcgctccgc acggcgattg gggggctgac tgcggtatga ttgtagagca accggccttc
    18481 tggctcgccg tcctacagat catctggatc gatattctat tatcgggcga caacgccgtc
    18541 gtcatcgcgc ttgcctgccg caagcttccg ccccggcagc gcatttgggg cgtcgttctc
    18601 gggacgatcg tcgccattac cttgcgggtg gtcttcgcgg gtatcgtgac gacgttgatg
    18661 caggttccct acctgaagct cgtcggcggc gtgctgctgt tgtggatcgc ggtaaagctg
    18721 ctcggtcccg aggagaacgg caagggcaac gaggttcagg ccgtcgacaa tctgtggcgt
    18781 gcgatcagga tcgtggcgat cgccgatgtg gtgatgagcc tcgacaacgt catcgccatt
    18841 gccgccgtcg ccgagcggtt cgaagcgccg cacaactacg cgctcctgat gttcggcctc
    18901 gccgtgagca tcccgctgat catggcgggt gccgccatcg tcatggcgct gctcgaacgc
    18961 tttcccgtcg tggtctgggc gggcgccgcg ctccttggct ggatcgccgg cgagatcatc
    19021 gccaaggacc ccttgttcac ggcgaatctc gatccgggca cggcgcatac gtgggagatc
    19081 gtcggcgcga gcttgggcgc actcttcgtc atcgccgcgg gcatgctgcg ccggcgctgg
    19141 cttcggcgga gcgcacccga ccagatctag ctttcgcgcc gggcctaggc gcggttggcg
    19201 ggatgcgcta gctgacgcac gctatatcgc caaacacctt cgagcagcga tagcttcgca
    19261 tcacgctcgc aaacgcttcg acaaaccgcc tcgggtgaga acggcggccg acgctcgcga
    19321 acgacgtcta ggccttcatg cgagcgatcg cgagcgtcat agcgcgtgat agcgtctgag
    19381 ctgccgcccg aaggatcaac aaacgctcac ggcaggctcg aggtcggttc tccgttctct
    19441 cgctcgcagg attatggttc gtaggcagaa aatcctagca cgtgatattt cgcaacagct
    19501 aagacgttgc ttggcgtgct aaagtcgggt caaatcaggc tgttagggag ttcaatcaaa
    19561 ctcttgggga gtccaagcgc tcgggggggg caatggacga ggatcagcga cacgaccgta
    19621 ggcaggtcct gaagatctac acgattggcg gtgtggcgac cgcgcttgtc atgccgacaa
    19681 gctggaccaa acccctcgta aaggccgttg tggttcccgc aatgcgcaag cgtcaccacg
    19741 cgacaaacgt cgtgacgacg agacgacgac cacgtccggt ccaacgacca ccacatccgg
    19801 tccaacgacc acgccgtcta acaccaccac gtccagcaca acttccacca ccagcacaac
    19861 ttccaccagc acgtccacca caacttccac cactacgacc agcacaactt ccaccaccac
    19921 gtccaccaca acttccacca ccaccacagc cgggcctcaa
//
LOCUS       DASUQP010000019         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_8130,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000019 DASUQP010000000
VERSION     DASUQP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8130"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFY74_00915"
     CDS             complement(<1..594)
                     /locus_tag="VFY74_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5930303.1"
                     /translation="MTGIHRAKYRHRLPQLDGGLFLTDGGLETTLIFHDGFALPMFEA
                     FVLADSEQGRAALRAYFDRYVATAVEHGTGFILESPTWRANPDWGQKLGYDPRRLAVV
                     NRAAIAMMGEIREVYETARTPIVISGAIGPRGDGYDPGRVMSPLEAEAYHAWQIGVFA
                     DAGADLVSAFTLTNVSEAMGVARAAKAAGMPCVISFTL"
     gene            819..1444
                     /locus_tag="VFY74_00920"
                     /pseudo
     CDS             819..1444
                     /locus_tag="VFY74_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597477.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1467..2699)
                     /gene="hemA"
                     /locus_tag="VFY74_00925"
     CDS             complement(1467..2699)
                     /gene="hemA"
                     /locus_tag="VFY74_00925"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX5930304.1"
                     /translation="MDYESRFRQRLEALHAEGRYRIFADLKRRCGAYPTARHFTGGHV
                     RDVTVWCSNDYLGMSQSPVVRNAMHEAIEEVGAGSGGTRNIAGTTHYHVELEAELADL
                     HGKEAALLFTSAYIANDSTLSTLQRLMPGLVMFSDEKNHASMIEGIRRGGGEKHIFRN
                     NDIGHLEELLKTVPALTPKIIVFESVYSMDGHISPIGTICDLAEKYGALTYLDEVHAV
                     GLYGPRGGGIAERDGVMHRVDIINGTLAKGFGVMGGYIAASAACCDAIRSYAPGFIFT
                     TSLAPAIAAGALASIRHLKRSEIERARHKERVKTVKGLMAEARLPVMENPSHIVPVMV
                     GDPVHCKAVTDTLLTQYRIYVQPINYPTVPRGTERMRFTPSPVHSNEQVAALIAALKE
                     LWMACPVANGKYVRLAAE"
BASE COUNT          494 a          903 c          918 g          505 t
ORIGIN      
        1 gagcgtaaaa gaaatcacgc atggcatccc ggcggctttg gctgcacggg cgacgcccat
       61 ggcctcgctg acattggtca gcgtgaaagc cgagacaaga tcggcgcccg cgtcggcgaa
      121 cacgccgatc tgccatgcgt gataggcttc ggcctccaat gggctcatca ccctgccggg
      181 atcgtaaccg tcgccacgcg gcccgattgc tccgctgatg acgatgggcg tacgggcggt
      241 ctcgtagacc tcgcggatct cgcccatcat ggcgatcgcg gcgcggttga ccacggcgag
      301 ccgccgagga tcgtagccga gcttctgccc ccaatcggga ttggcgcgcc aggtcggact
      361 ttccagaatg aaaccggtgc cgtgttcgac agccgtggcg acatagcggt cgaaataggc
      421 gcggagcgcg gcgcggccct gctcgctgtc ggcaaggacg aaggcttcga acatcggtag
      481 cgcaaagccg tcatgaaaga tcagcgtggt ctcaagtccg ccgtcggtga ggaacagacc
      541 gccatcgagc tgcggcaggc ggtggcgata tttggcgcgg tgaattccag tcatggtcaa
      601 gctccagcgt ttggggtggc gcgagcggcg cccggtgccg ggaagatggg atcggcggtt
      661 gggccgcacc agtgaactcg tggtgcagtg cggatgtcgg cctttggcct ggacgtgaca
      721 gggttaaccc ttctcgcggg gcttggcatg acggacccgc gcgttagcgc tccgtatcaa
      781 ctgtactcgt ggtaaagtcg gcatctggag agactgccat gcaggacgcg caggggcgga
      841 agggcggaga tacgcgcgag cgcattctcg atgcggccga atctgcggtg ctcgacaagg
      901 gattcgccgc cacctcgatc gaggaactga tcgcagccgt cgggattaca aagagcggat
      961 tcttctatca tttcaaggac aagggcgagc tgccaagacc ctcctggttc gctacatcga
     1021 gcgcgaggag gccctgttcg acgagatctt cgcccgcgcc gatgagctca acgaggatcc
     1081 gctgcatggc ttcctcgttg gcctcaagat gctgtcggag ctgatggccg acttgcccgg
     1141 cggccatccc ggatgcctca tcgccgccta ctgctatcag gaccgcctgt tcgaccggga
     1201 agtgcaggca ctcaacaccg ccgcggtgct cgcgtggcgc aaacggttcc gcgcgcgcct
     1261 cgacttgatc gccgcgcgct atccgccgcg gatcgcgatc gacctcgacg atctcgccga
     1321 catgctggcg gcgatcgccg atggcggcat catcctgtcc aaggtcgtca aggacgagga
     1381 cgctttggcg cgtcagttga tgctctaccg cgacttcgtt cgcgcggtct ttgtcggggt
     1441 ctgaaggagg acgcgcggcg acgccgctac tcggcggcga gcctcacata ttttccgttc
     1501 gcgaccgggc aggccatcca cagctctttc agcgcagcga tgagcgccgc gacctgctcg
     1561 ttgctgtgca ccggcgacgg cgtgaagcgc atccgctcgg tgccgcgcgg cacggtcggg
     1621 tagttgatcg gctgcacgta gatccgatac tgggtgagaa gcgtgtcggt caccgccttg
     1681 cagtgcacgg gatcgcccac catcaccgga acgatgtggc tcgggttctc catcaccggc
     1741 aggcgggctt ccgccatcag ccccttcacc gtcttgaccc gctccttatg gcgcgcgcgc
     1801 tcgatctcgc ttcgcttcag atgccggatc gaggcaagcg cgccggccgc gatcgccggc
     1861 gcaagcgagg tggtgaagat gaagcccggc gcatacgagc ggatggcgtc gcagcaggcg
     1921 gcgctcgcgg cgatatagcc gcccatcacg ccgaaaccct tggccagcgt cccgttgatg
     1981 atgtcgaccc ggtgcatcac cccgtcgcgc tcggcaatgc cgccgccgcg cgggccgtaa
     2041 aggccgaccg catggacctc gtcgagatag gtgagcgcgc catacttctc ggcgagatcg
     2101 caaatcgtcc cgatcggaga aatatgcccg tccatcgaat agacgctctc gaacacgatg
     2161 atcttgggcg tgagcgccgg gacggtcttc agcagctcct ccagatggcc gatatcgttg
     2221 ttgcggaaga tgtgcttctc gccgccgccc cgcctaatgc cctcgatcat cgaggcgtgg
     2281 ttcttctcgt cggagaacat cacgagcccc ggcatcagcc gctgcagcgt cgacagcgtc
     2341 gaatcgttgg cgatgtaggc gcttgtgaac agaagcgccg cctccttgcc gtggagatcg
     2401 gcaagctcgg cctcgagctc gacatgataa tgggtggtgc cggcgatgtt gcgggtgccg
     2461 cccgagcccg cgcccacttc ttcgatcgcc tcatgcatgg cgttgcggac aacggggctc
     2521 tggctcatgc cgagatagtc gttcgagcac cacaccgtga cgtccctgac gtggccgccg
     2581 gtgaagtgcc tcgccgtggg atacgccccg cagcggcgct tcagatcggc gaaaatccga
     2641 taccggccct cagcatgcag cgcctcaagt cgctggcgga agcgggactc gtagtccatg
     2701 cgaacctcga tcacctcaac ccgaaccggc ctctgcccag gccggaagcc gctggatggg
     2761 caggcgccca atgccggctt tgttcatcca aggagtagca tcaatcgcaa cgtcgtcaca
//
LOCUS       DASUQP010000020         6025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1236109, whole genome shotgun sequence.
ACCESSION   DASUQP010000020 DASUQP010000000
VERSION     DASUQP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236109"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFY74_00930"
     CDS             complement(<1..494)
                     /locus_tag="VFY74_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5930305.1"
                     /translation="MIIAQMMRMLLAAGLAWLGIGLAGLSAACDSEPQQSAEADAELL
                     DAMIGQMIMVGFDGRSERDPGVIAVRDQLAKGTIGGVVLYPRNIGSPEQLRALTAYLR
                     NAKSSPIPFIAVDQEGGVVQRLTPQNGHGYFPSARSVGANPSFASPEAAERLYATMAE
                     DLAGA"
     gene            complement(513..2198)
                     /locus_tag="VFY74_00935"
     CDS             complement(513..2198)
                     /locus_tag="VFY74_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX5930306.1"
                     /translation="MRLVVFFIGLVAVASVGAAQSLPTGPNRVPLGSSKPIGPRIKPP
                     SIKPIGPSIRLPGPRLIPPVTGRKCPPWTEGKWPDCKTIGEKKCPNNTTGKWPRCKPK
                     STASCADKGLIGKWPNCRTPDPKRCPKGTEGVWPACVDIAKPGGKECPEGQVRKGKKC
                     VQVTETGGGKTTPPKEPPRAAAKKQPAPVPPGVAALVTGRPHRSREILVLVKAARADE
                     IATGLAREHNVTAEPRVPVVLLDGVIVRLRLRQGQSLAALLAAISADPDVELAQPNYD
                     YKASEKASLPKGAPQYAGEAIKLEEAHRLARGRGVMIAVIDTAMDEAHPELAGAIAGK
                     FDAVGNGPARPEPHGTEIAGILAARAQLTGVAPEAKLLSVRAFSGGKAKPALSTSFQL
                     LKGIDWAFASGAKIMNMSFTGPQDPLLERMVKAAAEKGVIVIAAAGNEGPKAPPVYPA
                     AYPEVIAVTATDEKDKLYGKANRGGYVFIAAPGVDIAPGLKGSYGISSGTSMAAAHVS
                     GVVALLLERDGKLDWSGVRAILSKSARKPDSPLAAEDFGAGIVDAAGALGAPR"
     gene            complement(2202..2891)
                     /locus_tag="VFY74_00940"
     CDS             complement(2202..2891)
                     /locus_tag="VFY74_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930307.1"
                     /translation="MAVMREERLEKLGERDEIEALLPWYVSDKLDAKSRARVERYMDA
                     HPEVMAHLALAREEADATIASNEAITAPGRDALDRLRASIAAAPRRRPSADLLTQISD
                     RFADWLAGFSPPQLALAGAVAALLVLLQAAAIGTLIMERVTAPSYQTAGGEETAGGGY
                     ELLVGFSPTATAGEITELLKRLDAVLTDGPRAGLYRLRFPDAKSGDEDRKAAIEALEQ
                     SGMVTVVLPGR"
     gene            complement(2882..3448)
                     /locus_tag="VFY74_00945"
     CDS             complement(2882..3448)
                     /locus_tag="VFY74_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5930308.1"
                     /translation="MPAQAEARALLKAIASGDAKALERLFARTETRVFRFIMRVVKNQ
                     AIAEELLNEVFLTVWQNANRYEGRSEPLTWMLSIAHNKAISTLRKKTELLGIDEEASG
                     DLVAEEDTPDIAAQKHDKSVKIRACIAELSADHRTVLDLVYYQEQSVAEVAEVLGIPE
                     NTVKTRMFYARKKLSELLLARGMDRGWP"
     gene            3842..4375
                     /locus_tag="VFY74_00950"
     CDS             3842..4375
                     /locus_tag="VFY74_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930309.1"
                     /translation="MVSFRPTLCRSILAALLLMPQLAVAEPEQPTEEESADATFAKSF
                     LGKSYDGELEIEGWIDLGGGLVAPPIYIHQYLREEDGTYLVLTSREVAKAAADTPASF
                     VVTDALVVPKPQKDAAFSIACVQGEDATLKFMGEAKGKEDKEWWSDVRRAWEISLETG
                     EIASIKTKGVRCTNPNW"
     gene            4468..5556
                     /locus_tag="VFY74_00955"
     CDS             4468..5556
                     /locus_tag="VFY74_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX5930310.1"
                     /translation="MRLMTTAYINRIATAVPPNNVHAGFVRLAETQLPDDHRQRTVFR
                     RMAERSGIERRYSFLTPVPENDTEELDATAFYVRGRFPSTATRMRLFADKAPGLAASA
                     VERLELGSGRDRITHLLITCCTGFTAPGLDLELVERCKLPRSIERTIIGFMGCYAGIN
                     ALKLARHIVRSNPRARILVVNFELCTLHLQDTSDLAKLLTFLLWGDGCAASLVSADPN
                     GIALESFRAVYFPDTAELMRWDIGDSGFDMVLSGRVPAAIEDGIRIHGNEILEGADPR
                     AIELWAVHPGGRTVVDAVERAFAIAPSAMSASREVLREFGNMSSATVMFILKTLLADG
                     KPGSAGCGMAFGPGLIAETMLFRTGRVV"
     gene            5577..>6025
                     /locus_tag="VFY74_00960"
     CDS             5577..>6025
                     /locus_tag="VFY74_00960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5930311.1"
                     /translation="MTFPRRLETELLDVLPADDPDAVRSRRDLKLLNAVMLHPGIMAR
                     RMRHHAVAAPKRIVELGAGDGSLMLRLARRLARHWPGVKTILVDQQGIVAPATRQAVS
                     LLGWRQDQVTQDVFEYLRSAKPADIVIANLFLHHFKPPQIVEIFARCA"
BASE COUNT         1028 a         1972 c         1910 g         1115 t
ORIGIN      
        1 gcgcctgcga ggtcctcggc catcgttgcg tagaggcgct ctgccgcctc gggcgacgca
       61 aagctcggat ttgcgcccac gcttcgcgcc gaggggaaat acccgtgacc gttctgcggc
      121 gtgagccgct gcaccacgcc tccctcctga tcgaccgcga tgaacgggat cggcgaggac
      181 ttggcattgc gcagataggc ggtcagtgcg cggagttgtt ccggtgagcc gatattgcgg
      241 gggtagagca caacgccgcc gatcgtgccc ttcgccagct ggtcgcgcac ggcgatcacg
      301 ccgggatcgc gctcgctgcg cccgtcaaac ccgaccatga tcatctgccc gatcatggca
      361 tcgagcagtt cggcgtccgc ttcggcagat tgctgcggct cggagtcgca ggccgcgctg
      421 agcccggcaa gcccgatgcc gagccaggcg aggccggctg cgagcagcat cctcatcatc
      481 tgcgcaatga tcatgctgcg cggcgccaat gtctagcgcg gtgcgccgag tgcgccggcg
      541 gcgtcaacga tgccggcgcc gaaatcctcg gcggctaggg gagagtcagg cttgcgcgcg
      601 ctcttcgaga gtatcgcgcg gacgccgctc caatccagtt ttccatcgcg ctcgagcagc
      661 agtgcaacca cgcccgagac atgggccgcg gccatcgaag ttccggagga aattccatag
      721 cttcccttta gtccgggcgc gatgtcgacg cctggcgcgg cgatgaagac gtaaccgccg
      781 cgattggcct tgccgtagag tttgtccttc tcgtccgtag cagtgaccgc gattacctcg
      841 ggataggccg cggggtagac cggcggcgcc ttcggccctt cattgccggc ggccgcaatg
      901 acgatcacgc ccttctccgc cgcagccttg accatgcgct cgagcagcgg atcctgtggt
      961 ccggtgaagc tcatattcat gatcttggca cccgaggcaa aggcccaatc gattcccttg
     1021 agcagctgga aagaggtcga gagcgcgggc ttcgccttgc cgccgctgaa tgcgcgcacg
     1081 ctgagcagct tggcctcggg cgcaacgccc gtgagttgcg cgcgcgccgc taggataccg
     1141 gcgatctcgg tgccgtgcgg ctctggccgc gccgggccgt ttcccaccgc gtcgaacttg
     1201 ccggcgatcg caccggcaag ctcgggatgc gcttcgtcca tcgccgtatc gatcaccgca
     1261 atcatgaccc cgcggccgcg cgccaagcga tgcgcctcct ctaacttgat cgcctctccg
     1321 gcgtattgcg gcgcgccctt agggagcgaa gccttctcgc tggccttgta gtcgtaattg
     1381 ggctgcgcca gctcgacatc gggatcggcg ctgattgcgg ccaagagcgc cgcgagcgac
     1441 tgaccttgcc tcaacctgag ccgcacgatc accccgtcga gaaggacgac cggcacgcga
     1501 ggctcggcgg taacattgtg ctcgcgcgca aggcccgtgg cgatttcgtc ggcgcgcgcc
     1561 gctttcacca ggacaagaat ttcgcgcgag cggtgcgggc gaccggttac caaggcggcg
     1621 acccccggcg ggacgggggc aggctgcttc ttcgccgcgg ccctgggcgg ctccttcggc
     1681 ggcgtggtct tgccgccacc cgtttcggtc acctgcacgc atttcttgcc cttgcgcacc
     1741 tggccctccg ggcattcctt gccgcccggc ttggcgatgt cgacgcaagc cggccagacg
     1801 ccctcggtgc ctttgggaca acgcttgggg tcgggcgtgc ggcagttggg ccacttgccg
     1861 ataaggcctt tgtcggcgca gctcgccgtc gatttcggtt tgcatctggg ccatttgccc
     1921 gtggtgttgt tcgggcattt cttctcgccg atagtcttgc aatcgggcca cttgccttcg
     1981 gtccaggggg gacacttacg gccggtcacc ggcgggatga ggcgtggacc aggcagcctg
     2041 atgctcgggc ctatgggctt gatgctcggg ggcttgattc tgggaccgat aggcttgctc
     2101 gagccgagcg gaacgcggtt cggaccggtc ggcagggact gcgcggcgcc gaccgacgcc
     2161 accgccacaa gacctatgaa gaacacgacg agccgcatcg cttaccgtcc cggcaaaacg
     2221 accgtcacca tccccgattg ctctagagcc tcgatcgcgg ccttacgatc ctcatcgccg
     2281 gactttgcat ccggaaagcg caagcgatag agaccggctc tcggcccgtc ggtcaggaca
     2341 gcatcgagcc gtttgagcag ttcggtgatc tcgccggcgg tcgccgttgg ggagaacccg
     2401 acaagaagct cgtagccccc gcccgcggtc tcctcgccgc cggcggtctg ataggacgga
     2461 gcggtaacgc gttccatgat gagggttccg atggccgctg cttgcagcag gacgaggagc
     2521 gcggcaacgg ccccggcgag cgcgagctgc ggcggcgaaa agccggcgag ccaatcggcg
     2581 aagcgatccg agatttgcgt gagcaggtcc gctgaaggcc tgcgccgtgg cgcggccgca
     2641 atgctcgccc gcaagcggtc gagagcatcg cgccccggtg cggtaatcgc ttcgttggaa
     2701 gcaatggtgg cgtcggcctc ttcgcgggcg agcgccaaat gcgccatcac ctcgggatgc
     2761 gcgtccatgt agcgttcgac acgcgcgcgc gatttcgcat cgagcttgtc cgagacgtac
     2821 cagggcaaga gcgcctcgat ctcgtcgcgt tcgccgagct tctcaagcct ctcctctctc
     2881 atcacggcca tcctcgatcc atgccacggg cgagaagcag ctccgacagc ttcttccgtg
     2941 cgtagaacat gcgcgtcttc actgtgttct cgggaatgcc gagcacctcg gcgacctccg
     3001 ccaccgattg ctcctggtaa tagacgaggt cgagaacggt acgatgatcg gccgacaatt
     3061 cggcgatgca ggccctgatc ttgacgctct tgtcgtgttt ctgcgcggca atgtcgggcg
     3121 tatcctcctc ggccacgaga tcgccggagg cctcctcgtc aattcccaag agctccgtct
     3181 tcttgcgcaa ggtgctgatc gccttgttgt gcgcgatcga cagcatccag gtcagcggct
     3241 cggaccgtcc ctcgtagcgg tttgcgttct gccacacggt caagaatacc tcgttcaaga
     3301 gctcctcggc gatcgcctgg ttcttcacca cgcgcatgat gaagcggaac acgcgggtct
     3361 cggtgcgagc gaagagacgt tccaaggcct tggcgtcacc cgacgcgatg gccttcagca
     3421 gggcccgcgc ttcggcttgc gcaggcatgg gcatcaccgc cgctcgcgaa accttggacg
     3481 ccgcgaaaag tcagaaggtt caaatgcggc aagccgggta tcgccgaaag attgccagat
     3541 cagccggcaa aagaacaggc aagcgcggcc atcctgaccc tgacgcgtcg ctccgtgagc
     3601 gcacccgtga tcggaattcc acaatacgtt caatatctta agcggattgt ggaccacgcg
     3661 ccaacctttg aaccaacaga agcgttgcgc ggcgatctct ccttccacgc ctcgggcatt
     3721 ccggattgtg acccgatctc gggcttggtc ccgggtgcgg aacgcgccac ggcggtgccg
     3781 atttcgtcac gattatgagt agactgaagg ccgcaataag acccgcgccg agggaactct
     3841 tatggtcagc ttccgcccta ccctgtgccg ttcgatcctt gccgccctgc tgctgatgcc
     3901 ccaactggcc gtggccgagc cggagcaacc gaccgaagag gagtccgccg acgcgacctt
     3961 cgccaagagc tttctcggca agagttatga cggcgagctc gaaatcgaag gctggatcga
     4021 tctcggcggc gggctggtcg ccccgccaat ctacatccac caatatctgc gcgaggagga
     4081 cggcacttat ctcgtgctca cgagccgcga agtggccaag gcggccgccg acacgccggc
     4141 atcctttgtc gtgaccgacg cgctcgtcgt gccgaagccg caaaaggacg cggcgttttc
     4201 catcgcctgc gtgcagggcg aggacgcaac cttgaaattc atgggcgagg cgaaaggaaa
     4261 agaagacaag gaatggtgga gcgatgtacg ccgcgcgtgg gagatttcgc tcgagaccgg
     4321 cgagatcgca tcgatcaaga ccaaaggggt tcgctgcacc aatcccaact ggtgagcgag
     4381 gaacgcttgg gctgagcccg caagggatct gtctcgcctt agatcgaccg aatagccgtc
     4441 ctacgctggg tttctttgcg gagaaggatg aggctcatga ccaccgccta catcaaccgc
     4501 atcgcgaccg cagtgccgcc caacaacgtg cacgcgggct tcgttcgcct tgccgagacg
     4561 cagcttccgg acgatcaccg ccagcgcacc gtattcaggc gcatggcgga acgatcgggt
     4621 atcgaacgcc gctattcctt cctgacgccg gtgccggaga acgatacgga ggagctcgac
     4681 gcgaccgcgt tttatgtcag gggccggttc ccgagcacgg caacgcgcat gcggctcttt
     4741 gccgacaagg ccccgggtct cgccgcttca gccgttgaac ggctcgaact cgggagcgga
     4801 cgcgatcgca tcacgcatct cctcatcacc tgctgcaccg gcttcacggc gccgggtctc
     4861 gatctcgagc ttgttgaacg ctgcaagctg ccccgctcga tcgagcggac gatcatcggt
     4921 ttcatgggct gctatgccgg gatcaacgcc ctcaagctcg cgcgccacat cgttcgctcg
     4981 aatcccaggg cgcgaatcct ggtcgtgaac ttcgagctct gcacgctgca tcttcaggat
     5041 acgagcgatc tcgccaaact tctgactttc cttctctggg gcgacggctg cgccgcatcc
     5101 ctggtcagtg ccgatccgaa cggcatcgcc ctcgagagct tccgcgccgt gtattttccc
     5161 gacaccgccg agttgatgcg gtgggatatc ggcgacagcg gattcgacat ggttctctct
     5221 ggcagggtac cggcggcgat cgaggacgga attcgcattc acggcaacga gatcctggaa
     5281 ggcgccgatc cgagagcgat cgaactctgg gcggttcatc ccggcggccg gaccgtcgtt
     5341 gatgccgtcg agcgcgcctt cgccatcgcg ccttcggcga tgtcggcgtc acgggaggtt
     5401 ctccgcgaat tcggcaacat gtcgtctgcg accgtcatgt tcatcctgaa gacgctgctc
     5461 gctgacggca agccaggttc ggccggctgc ggcatggcat tcgggcccgg cctcatcgcc
     5521 gagacgatgt tgtttcgaac gggccgtgtc gtttgagcga agaggattga ggtaggatga
     5581 ccttccctcg ccgcctcgaa accgagctcc tcgacgttct gccggccgat gatcctgatg
     5641 cggttcggtc gcgccgcgac ctcaagctcc tgaacgcggt gatgctgcac cccgggatca
     5701 tggcgcggcg catgcgccat catgccgttg ccgcgccgaa acggatcgtc gagcttggtg
     5761 cgggcgacgg cagcctgatg cttcggctgg cccggcgcct cgcgcggcac tggccgggcg
     5821 tcaagaccat cctcgtcgac cagcaaggca ttgtcgctcc ggcgacgcgg caggcggttt
     5881 cgctactcgg ctggcggcag gaccaggtca cgcaagacgt gttcgaatat cttaggagtg
     5941 cgaaaccggc cgacatcgtc atcgccaatc tcttcctcca tcacttcaag cctccgcaga
     6001 ttgtggaaat tttcgctcga tgcgc
//
LOCUS       DASUQP010000021         4849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_827821,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000021 DASUQP010000000
VERSION     DASUQP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827821"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /gene="tsf"
                     /locus_tag="VFY74_00965"
     CDS             <1..520
                     /gene="tsf"
                     /locus_tag="VFY74_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006589391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5930312.1"
                     /translation="EMIGTIGENMTLRRTGYLAVKNGTVASYMHNTLAPGLGKIGVIV
                     ALESKGEPKQLAAFGKQLAMHIAAANPQAVDVESLDKDVIARERAVLTEQAKDSGKPA
                     PVIEKMVEGRLRKFYEDVVLLAQAFVHDPETKVENAVADAEKAAGAPIKIVGFHRFAL
                     GEGVDRAGGEAA"
     gene            596..1339
                     /gene="pyrH"
                     /locus_tag="VFY74_00970"
     CDS             596..1339
                     /gene="pyrH"
                     /locus_tag="VFY74_00970"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5930313.1"
                     /translation="MAGTGAANNLRYRRVLLKLSGEALAGAKGYGIDLDVLAPITRDI
                     AAAVAGGAEICVVVGAGNIYRGLMASEGGMDRARADYMGMLATVMNGLALQAALERQG
                     ATARVLSAIPMPTVCEAYIRDKALDHLAKGRVVIFAGGTGNPYFTTDTAAALRAAEMG
                     CDALLKATQVDGVYSADPRADRSARRYDRLSYDDVLKQDLRIMDGPAIALARDNRIPI
                     IVFSIKSQGGLADVLKGKGASTTIDGGGA"
     gene            1428..1985
                     /gene="frr"
                     /locus_tag="VFY74_00975"
     CDS             1428..1985
                     /gene="frr"
                     /locus_tag="VFY74_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5930314.1"
                     /translation="MTSFDLKEIERRMRGAVSVLKQEFGGLRTGRANASLLDPITVTA
                     YGNAMPLNQLATINVPEPRLITVQVWDRSQVGAVDRAIREANLGLNPVVDGQLLRLPI
                     PELNQERRQEIAKVAHKYAEQARVAVRNVRRDGMEHLKRAEKEHEMGKDEHHGLSTKV
                     QDLTDKIIKEIDEALASKEAEIMQV"
     gene            2030..2794
                     /locus_tag="VFY74_00980"
     CDS             2030..2794
                     /locus_tag="VFY74_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX5930315.1"
                     /translation="MTLAADKLAWQAAGRPAVRHIAIIMDGNGRWAAERGLPRAEGHR
                     QGVESVRRTVEAALDLGITHLTLFSFSSENWARPKQEINDLFGLLRRFIRRDLADLHK
                     NGVKIRVIGSRTGLEPDLLRLIDDAVELTKNNTALNLTVAFNYGARDEIARAARRVAE
                     AVSNGSLVPSDVTEERFGSYLDTADLPDPELLIRTSGELRLSNFLLWQLAYAEFVFVD
                     TYWPDFSREQLEAAIAEFYRRNRRFGGTSARSTAWL"
     gene            2827..3621
                     /locus_tag="VFY74_00985"
     CDS             2827..3621
                     /locus_tag="VFY74_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5930316.1"
                     /translation="MLRIVSAAALAALALGATILSPWTLLVLVIAAGGLIAWEWGRLT
                     RGNSLDATALLAAGSVTVLAISVAVGRPAYGFFFLLAAAAATIWFTGGAFSRAWALGG
                     LLYAALPASSLVFLRGDEMLGAAAVIYLFIVAWTTDTASYAAGHFIGGPKLAPRISPH
                     KTWSGFLAGVLAPSLVGIVFATVLKSGSAVRLALVSVGLALACQAGDLLESWVKRRFG
                     AKDSSQLIPGHGGLLDRIDGLMMAAVLAGLIALRNPAAPGHSLLVW"
     gene            3634..4848
                     /gene="dxr"
                     /locus_tag="VFY74_00990"
     CDS             3634..4848
                     /gene="dxr"
                     /locus_tag="VFY74_00990"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5930317.1"
                     /translation="MFPSVTPSVEIEPRGARRRRISVLGATGSIGENTLDLIGREPAA
                     YHVIALTGGHNAARLAELAIQHRAELAVIADAEAYPALRQALSGTGIEAGAGEAALIE
                     AASRPADWVMAAIVGAAGLKPTLEAVRQGRLIALANKECLVSAGDIFMAEVTRAKATL
                     LPVDSEHSAVMQVMAGVQPERIERVCLTASGGPFRNWSLDAMRVARSEQALNHPNWSM
                     GPKVTIDSATLMNKGLELLEAHHLFSLPAAKLDVLIHPQSIVHCLVHLSDGSVLAQMS
                     CPDMRTPIAYSLAWPERMHAPTKRLDFAALGALTFEVPDVQRFPALRLAREVLAAGGS
                     AGTVLNAANEVAVEAFLGGKIGFLAIAGLVETTLESTAELIGRHRRGLEDVLAIDTEA
                     RAKANGLLARFA"
BASE COUNT          830 a         1539 c         1610 g          870 t
ORIGIN      
        1 ggagatgatc ggcacgatcg gcgagaacat gacgttgcgg cgcaccggct acctcgccgt
       61 caagaacggc accgtggcga gctacatgca caacacgttg gcgccgggtc tcggcaagat
      121 cggcgtcatc gtggcgcttg agtccaaggg cgagccgaag cagctggcgg cattcggcaa
      181 gcagctcgcc atgcatatcg cagctgccaa cccgcaagcc gtcgacgtcg aaagcctcga
      241 caaggacgtg attgcgcggg agcgcgccgt gctcaccgag caggccaagg attccggcaa
      301 gcccgcgccg gtcatcgaga agatggtgga agggcgcctg cgcaagttct acgaggacgt
      361 ggttctgctt gcgcaagcct tcgtgcacga tccggagacg aaggtggaga atgccgtggc
      421 cgacgccgag aaggcggcgg gcgcaccgat caagatcgtc ggtttccatc gattcgcgct
      481 cggcgaaggc gtcgatcgcg cgggcggcga ggcggcgtag gatgggcagt cattaggccg
      541 cgtttggcat agggcgcgcg ggatcggagt tcgatcgcga gcgggaggaa ggttgatggc
      601 agggacagga gcggccaaca accttcgcta tcggcgggtg ctgttgaagc tctcgggcga
      661 agcccttgcc ggcgccaaag gctacggcat cgatctcgat gtgctcgccc cgatcacgcg
      721 cgacattgcc gcggcggtag ctggcggcgc ggagatctgt gtcgtcgtcg gtgccggcaa
      781 catctatcgc ggcctgatgg cgtccgaagg cggcatggac cgggcgcgtg ccgactacat
      841 gggcatgctc gccacggtca tgaacggtct cgcgctccag gccgcgctcg agcggcaggg
      901 ggcgacggca cgggtcttgt ccgccatccc catgccgacc gtgtgcgagg cctatatccg
      961 cgacaaggcg ctcgatcatc tcgccaaggg gcgggtggtg attttcgccg ggggcaccgg
     1021 caatccctat ttcaccaccg acacggcggc ggctctcagg gccgccgaga tgggctgcga
     1081 cgcgctgctg aaggcaaccc aggtggatgg cgtctacagc gccgatccgc gagccgaccg
     1141 gagcgcgcgc cgctacgacc ggctttccta tgacgacgtt ctcaagcagg atttgcgcat
     1201 tatggacggg cccgcgatcg cgcttgcccg agacaaccgg attccgataa ttgtcttttc
     1261 catcaagagc caaggcggct tggcggacgt gttgaaaggg aagggcgcct cgaccacgat
     1321 cgatggcgga ggcgcctgag gaagcacatg ggcgaattcg cggtaccgat tgggggaccc
     1381 aagggcctcg cccaaggcgc taccaggagc gaaggcaggg acgctacatg acgagcttcg
     1441 acctgaagga gatcgagcga cgcatgcgcg gcgccgtcag tgtgctgaag caggagttcg
     1501 gcgggctccg caccggtcgc gcgaatgcga gcctgctcga tcccattacc gtgacggcgt
     1561 atggcaacgc catgccgctc aatcagctcg ctaccatcaa cgttcccgag ccgcgcctaa
     1621 ttaccgtgca agtttgggac cggagccagg tcggcgccgt tgatcgcgcc atcagagagg
     1681 caaatctggg gctcaatccc gtggtcgacg gccagctcct gcggttgccc attccggagt
     1741 tgaaccagga gcggcgccag gagattgcca aggtcgccca caaatacgcc gagcaggcgc
     1801 gggtggcggt gcgcaatgtg cgccgcgacg gcatggaaca tctcaagcgc gccgagaaag
     1861 agcacgagat gggcaaagac gagcatcacg gcctttcgac caaggtgcag gatctgaccg
     1921 acaagattat caaggaaatc gatgaggccc ttgcgagcaa ggaagccgaa atcatgcagg
     1981 tatagggctt tcaggacatg gcgtttccct tcatctggag cagccgaacg tgacgcttgc
     2041 ggccgataag cttgcttggc aggcggccgg gaggccggcc gttcgccaca ttgcaatcat
     2101 catggacggc aacggccgct gggccgccga gcgcgggctg ccgcgcgccg aaggccatcg
     2161 ccagggagtg gaaagtgtca ggcgcacggt cgaagctgcg ctcgatctcg gcatcaccca
     2221 tctcacgctt ttcagcttct cgtcggagaa ctgggcgcgc ccgaagcagg agatcaacga
     2281 tctgttcgga ttgctgcgcc gtttcatccg ccgcgacctg gccgatcttc acaagaacgg
     2341 cgtgaagatt cgtgtgatcg gcagtcgtac cgggctcgag cccgaccttc tccggctgat
     2401 tgacgacgcc gtcgagctca ccaagaacaa caccgcgctc aacctgaccg tcgccttcaa
     2461 ttacggcgcc cgcgacgaga tcgctcgcgc ggcgcgccgc gtcgccgagg ccgtgtccaa
     2521 cgggtctctc gttccttccg acgtgaccga agagcgcttc gggtcctatc tcgacactgc
     2581 cgatcttccc gatccggagc tcctcatccg cacgagcggc gagctccgcc tgtcgaactt
     2641 cctcctctgg cagctcgctt atgccgaatt cgtgttcgtc gacacgtatt ggccggactt
     2701 ctcgcgcgag cagttggaag cggcaatcgc cgaattctat cggcgcaatc gccgctttgg
     2761 cggcacttcg gcgagatcga ccgcgtggct ttgacgggag cgcagtccag ccgacagcgt
     2821 gaaatcgtgt tgcgcattgt ctcggccgcg gcgcttgcgg ccttggcgct cggggccacg
     2881 atcttaagtc cgtggaccct tctcgttctg gtcatcgcgg cgggaggcct cattgcctgg
     2941 gagtggggac ggctgacgcg cggcaacagt cttgacgcga cagccttgct cgcagcgggc
     3001 agcgtcaccg tgcttgcgat ctccgtcgcc gtcggccgtc ccgcttacgg cttttttttt
     3061 ctgcttgccg cggcggcggc aacgatctgg ttcacgggcg gcgctttcag tcgcgcctgg
     3121 gcactcggtg ggctcctcta cgctgcgctc cctgcctctt ccctggtctt tcttcgcggc
     3181 gatgagatgc ttggcgccgc ggccgtgatc tatctcttca ttgtcgcctg gaccaccgac
     3241 acggcctctt atgccgccgg gcacttcatc ggcggaccga agcttgcgcc ccgcatttcc
     3301 ccgcacaaga cttggagcgg ttttcttgcc ggcgtccttg cgccgtcgct cgtcggcatc
     3361 gtctttgcca cggtgctcaa gagcggctcg gccgtgcggc ttgcgctggt aagcgtgggc
     3421 cttgccctgg cttgccaagc cggagatttg ctggagagtt gggtgaagcg ccgctttggc
     3481 gccaaggact caagtcagct gattccgggc catggcggct tgctcgaccg catcgatgga
     3541 ttgatgatgg ccgccgtgct tgcggggctc atcgcgctcc gcaaccctgc tgcccccggg
     3601 cacagcctgc tcgtctggtg aaagtcggtc cccgtgtttc cgtccgttac accctccgtt
     3661 gagatcgaac cgagaggcgc gcggcggcgg cgtatctccg tgcttggcgc aaccggctcg
     3721 atcggcgaaa acacgctcga tctcatcggc cgggagcccg cggcctatca cgtgatcgcc
     3781 ttgaccggcg gacacaacgc ggctcgcctt gccgaacttg caattcaaca tcgggccgaa
     3841 ctcgcggtga tcgccgacgc ggaagcgtat ccggcgttgc gccaggcgct gtcaggcacc
     3901 ggcatcgagg cgggcgcagg ggaggcggcg ctcatcgagg cggcaagccg ccccgcggat
     3961 tgggtgatgg cggcaatcgt cggcgcggcg ggtttgaagc cgacgctcga agcggtgcgt
     4021 caagggaggc tgatcgcgct cgccaacaag gagtgtctcg tctccgcggg cgacatcttc
     4081 atggccgagg tgacgcgcgc caaggcgacc cttcttcctg tcgattccga gcactcggcc
     4141 gtcatgcaag tgatggcggg cgtgcagcct gaacgaatcg agcgcgtttg cctcacggcc
     4201 tccggcggcc ccttccgcaa ctggagcctc gatgcgatga gggttgcgcg gtccgagcaa
     4261 gccctcaatc atccgaactg gtccatgggg ccgaaagtca cgatcgattc ggcgaccctc
     4321 atgaacaagg gtctcgagct cctggaagcg caccacctgt tctcgcttcc tgcggcgaag
     4381 ctcgacgttt tgatccaccc gcaatcgatc gtgcactgcc tcgttcatct gagcgacggg
     4441 tcggtcctgg cgcagatgag ctgccctgat atgcgaacgc ccattgccta cagccttgcc
     4501 tggccggaac gtatgcatgc gccgaccaag cgcctcgact ttgccgcgct cggcgcgctg
     4561 accttcgagg tcccggacgt gcaacggttt ccggcgctcc gtctggcccg cgaagtcctt
     4621 gccgcgggcg gaagcgcggg caccgtgctc aacgccgcga acgaagtggc ggtcgaggcc
     4681 tttctcggcg gcaagatcgg tttcctcgcc atagcgggcc tggtcgaaac gacgctcgag
     4741 tcgaccgccg agctgatcgg acgccatcga cgcgggctgg aggacgtgct tgccattgac
     4801 acggaagcga gggcgaaggc caacggattg ctcgcgcggt ttgcataaa
//
LOCUS       DASUQP010000022        13560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_419564,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000022 DASUQP010000000
VERSION     DASUQP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13560
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419564"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFY74_00995"
     CDS             complement(<1..197)
                     /locus_tag="VFY74_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5930318.1"
                     /translation="MDRRIFENRKVIAVEMQGHGRTADINRDFSYENLANDIAAMLDY
                     LKIKQADLLGYSMGGGVAMQVA"
     gene            complement(500..901)
                     /locus_tag="VFY74_01000"
     CDS             complement(500..901)
                     /locus_tag="VFY74_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX5930319.1"
                     /translation="MSDEAKIRDLIEAWAKAVRAKEIEGVLAHHTEDIVMFDVPPPVV
                     VKGLDAYRQTWPGFFKWQREGDGSFDIVSLDITAGDDVAFATAVLRCGSKQELKQDDT
                     PRLRLTVGLRKDNGRWRVAHEHHSFPLEHSA"
     gene            complement(937..1359)
                     /locus_tag="VFY74_01005"
     CDS             complement(937..1359)
                     /locus_tag="VFY74_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5930320.1"
                     /translation="MAQTAAHRREEDCADEQKNWTHGTFIWNELMTRNVEQAKRFYGD
                     TIGWRFEAMKMPDGRNYWCAMESGKPIAGIFSIDAPEFDGVPESWMSYLAVDDVDKRV
                     ASAVKASAKLMKPIFDVPGAILMEPGGAGIGWMTPVAK"
     gene            complement(1380..1826)
                     /locus_tag="VFY74_01010"
     CDS             complement(1380..1826)
                     /locus_tag="VFY74_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5930321.1"
                     /translation="MPAQASLAIKPSLTLKRRLNAPPEKVYAAWTDAEKVLKWFGPDS
                     GPVTQAVTDVRVGGRYAVTFSTEDGEVHHVSGIYREVVPNRKLVFTWAWRTMPERESL
                     VTVLIKPDETGSILTLVHEQFFDEPARDRHREGWTGCLDKLESYLA"
     gene            complement(1715..2152)
                     /locus_tag="VFY74_01015"
     CDS             complement(1715..2152)
                     /locus_tag="VFY74_01015"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5930322.1"
                     /translation="MVKYQATLDRTFAALADPTRRTLLAQLDRSERVSVSELARPFSM
                     SLPAVMKHLDVLSDAGLITRNKVGRTVTCQLAAAPMEQAMHWLNHYQRFWSDRLDRLA
                     AFVEEDQCPPKPASPSSPASRSNVVSMHRRKRSTRPGPTPKKS"
     gene            2338..2463
                     /gene="ykgO"
                     /locus_tag="VFY74_01020"
     CDS             2338..2463
                     /gene="ykgO"
                     /locus_tag="VFY74_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX5930323.1"
                     /translation="MKIKNSLRALRKRHRNNRVVRRRGRLYVINKTNRRYKARQG"
     gene            2470..3315
                     /locus_tag="VFY74_01025"
     CDS             2470..3315
                     /locus_tag="VFY74_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5930324.1"
                     /translation="MRTSRGVRLSLRNASVFRLAALLFCASFYFASPAASFESEPETP
                     ADVEEPADVAPDGIGAPSIETFPSEDLTEPRQFDRGLFRPDLKPGDEGATEPQPAGES
                     KEDRLGATPDDLPLPSPAEKPKVLGQLYVQLGKAKDAEAAVPIMTDIETLWRVTGSPT
                     VDLLIGRAELFTKGKDLDLALQILDATVAMAPEEAEAWYLRAKVYYLQSKYDLALADL
                     KRALDRDPKHYRALEDLGLVYDAFGRKPDALEAYRKALAVNPFLKDAGLAVQFLEKEV
                     GARNL"
     gene            complement(3324..4754)
                     /gene="pyk"
                     /locus_tag="VFY74_01030"
     CDS             complement(3324..4754)
                     /gene="pyk"
                     /locus_tag="VFY74_01030"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5930325.1"
                     /translation="MRRNRRVKIVATLGPASNNEETMRRMFEAGADVFRVNMSHTPHD
                     GLGDIHAIVRKLETEFSRPIGLLVDLQGPKLRIGKFDSGAIKLTEGDVLSFVRRESVG
                     GLGRVHLPHPEIFQAVQPGHSLLLDDGKFKLRIVEASDVRIDAEVMTSGLLGSRKGIS
                     LPDTVLPFGAMTEKDRADVDFAMSLGVAWIALSFVQRAEDIAEARKLACGRSSIMAKI
                     EKPSALDHLEEIIELADAIMVGRGDLGVEMPVEKVPGLQKQITRAARNAGKPVVVATQ
                     MLESMIFAPAPTRAEVSDVATAVFEGADAVMLSAESAVGKYPVEAVATMDRVAIEVER
                     DPLYDAIIHAQRIGPEATGADAISAAARTVAETLNLAAIVCYTASGATSLRAARERPK
                     QPIIALTPIPATGRKLALVWGLHCVLTDDPRDLDDMVAKACRIAYQEGFALPGQRVII
                     SAGVPLGTPGATNMLRIAFVGNDSDF"
     gene            complement(4751..5173)
                     /locus_tag="VFY74_01035"
     CDS             complement(4751..5173)
                     /locus_tag="VFY74_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEX5930326.1"
                     /translation="MLAGLVLASAGVTVGASTAEADLKLCNTTASRVGVAIGYKDAEG
                     WATEGWWNIPAHACETLLKGVLIAQFYYIHAVDYDRGGEWGGSLYMCTDDKSFTIRDT
                     KDCVKRGYKRTGFFEVDTGEERDWTVRLTDPEGEPKSQ"
     gene            5511..6614
                     /locus_tag="VFY74_01040"
     CDS             5511..6614
                     /locus_tag="VFY74_01040"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEX5930327.1"
                     /translation="MGRRPYRSIDGDARTGLLILCDHAENIIPPAHDRLGLKEQDLHR
                     HIAYDLGAAAVAERLAEMLGAPAILSRFSRLLIDPNRGLDDPTLVMQVTDGLVVPGNV
                     GLDAEALQSRIDTYYSPYHQAIERAVDSAIGLGKPPVIVSVHSFTQAWKGVARPWTVG
                     VLWDQDPRLALPLLAALRAIPGIAVGDNAPYSGQLKGDTLYRHGTGRGLAHALIELRQ
                     DLILGPEGQAEWAMRLAEVLRTVLDAGEAALHAIELHGSYTDGAGSTRRPLGRTERPV
                     MDEKTKTEIEAEAFRRLVQHLRERTDVQNIDLMELAGFCRNCLANWYRDAAAARGLPL
                     SKEEAREAVYGMTYEEWKAKYQSEAPKPLRQSG"
     gene            6742..6999
                     /locus_tag="VFY74_01045"
     CDS             6742..6999
                     /locus_tag="VFY74_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEX5930328.1"
                     /translation="MQLQTSAKDQLRAFVARIERLEEEKAALAADLREVYAEAKGNGF
                     DAKALRSVIRLRKQDEDKRREEEAVLATYLHALGMAEAEAA"
     gene            complement(7027..8310)
                     /locus_tag="VFY74_01050"
     CDS             complement(7027..8310)
                     /locus_tag="VFY74_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX5930329.1"
                     /translation="MRIRGSVARRAVLGASLTFLAAATWCTATAYRVSAGETRTLSMY
                     NIHTKETLTVTFKRDGKYDEAALKQINTFMRDWRANQETKMDPVLIDLVWTLQKQLGS
                     QKPIHLISAYRSAATNASLRRKGGGQAKKSQHIQGKAADIHFPDVPVKTLRNSALVQE
                     VGGVGYYPTSGIPFVHVDTGKIRMWPRIPRLELAALFPDGKTRYLPSDGKPITSRDYK
                     LAMAKGLVGKTMVASADTKPDVDVIAEAELDAETEVEMPQVILASYTPGAADIVEDSR
                     APNPPGRLFTYASVGGMSLPIPSLLRPAPKEAAPSAQTARLPAYEDAEVVGAPEADED
                     HPDQLSYVPFEIAGLMTDASVTYSRTVAPLAHPEQRSVEYLFEGMDHPTAFKLRQSSG
                     YQGLAAVQRFSGQAVKSLYAEVEPAAPTRLAQSTR"
     gene            complement(8527..10215)
                     /locus_tag="VFY74_01055"
     CDS             complement(8527..10215)
                     /locus_tag="VFY74_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5930330.1"
                     /translation="MSFICRVALTLLLAASMSGPGVAAEPDSPEALITRTEEIRIAVQ
                     QRLAAKFTTATETKKSEQGALVEFYAAPEQRLLWVDESGVNDRGKAVIAEIEKADDYG
                     LRASDYALPARDTFNAAAADAAAWLAEAEIKISYAVLGYARDARGGRIEPRRLSRNLD
                     PSLALPNPSEVIESIAFRSDPAAYLRSFQPDQPGFEALRQKLLELRGGKDSASTSAVA
                     IPDGPVLKLGVEHEHVALLRKRLDMPGERPDGTPVIETRFDLQVAHAVRRFQLAHGAF
                     PDGAVGSGTRRLLNGGKRSHKAGSPAQIKTVLVNMERWRWLPRDLGTFHVTVNIPEFM
                     LRLVKDEAPVYATRVVVGKPRTQTPIFSDEMQTVVFGPYWHVPASIKVDEIRPYLRQE
                     ASWFFRRGRWNTSVLQRHGLGIRYAGRDIDPDSIDWDQVDIRNLELYQPPGPGNVLGK
                     VKFVFPNKHDVYMHDTQQKYLFGYPVRAESHGCMRVQNPGELAAIIMKHDLGWSPART
                     YSALETGYDQQVSLRQTIPVHITYFTLKVNPDGSIVTYGDLYGHDSRMAAALGL"
     gene            complement(10319..11827)
                     /locus_tag="VFY74_01060"
     CDS             complement(10319..11827)
                     /locus_tag="VFY74_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5930331.1"
                     /translation="MPKRVLVVDDDPSQRRILEQAIERFGYPVATADSGDEALAALNA
                     DTAGDIGLVLLDLFMPGTDGLAVLSAMRVLPRKPPAIVQTSHGGIAAAIAAMRAGAVD
                     FVVKPVTPERLEVSIKNALKIEALTDELVRIKTSARCALGFADLIAGSDVMTRVLELG
                     RRAADSTIPVLIEGESGVGKELLARAVHGESARKSGPFVSVNCGAIPEHLVESILFGL
                     EKGALTGAPEKRIGKFVEADQGTLFLDEIGELTRDAQAKLLGALQDGEIDAVGAKHPV
                     KVDVRVIAATDRNLIDLATEGLFREDLYYRFNVLPIWVPPLRMRLDDIPELVRHFIAR
                     FAAEEGKRIDGIDAVAIAMLQRYSWPGNIRQLENAVFRAVVLTDAPVLTVNEFPQIAA
                     HVAGYAATVPAAPAPIDPAPRIAGPVMLGGSSGTPLTINVPQANGKDAIGILALSDTG
                     DIRPLEAVEADMIRLAIGRYRGRMTEIARRLGIGRSTLYRKMREIGLEVRAN"
     gene            12095..>13560
                     /locus_tag="VFY74_01065"
     CDS             12095..>13560
                     /locus_tag="VFY74_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5930332.1"
                     /translation="MPFKVRNWPEHPAFAATASARGKAKAETQSEAEGTSNLGALPEW
                     KLADLYERPNAPKLEADLAAAEREANAMQDRYAGKLAKLLDGGKGGAALAEAVRTFEA
                     LNDLMGRIVSYASLLYAADTSDPKRQKFFGDIQEKITAISSKLLFFPLELNRLDDAKL
                     ETAMSKSVLGHYRPWLEDLRKEKPYQLEDKLEQLFHEKAVTARGAWDRLFNETMTSLR
                     FETDGEKLSLEPTLNRLLDQDEAKRRDAAESLAKVFKENVRLFTLITNTLAKDKEISD
                     RWRGFADVADSRHLNNRVEKEVVDTLVAAVRDAYPRISHRYYAMKAKWLGKEALAHWD
                     RNAPLPEEPKQVISWADAQKTVLGAYRNFSPRMADIAGEFFAKGWIDAPVREGKSPGA
                     FSHPTVPSAHPYILLNYQGKPRDVMTLAHELGHGVHQVLANVQGPLMAPTPLTLAETA
                     SVFGEMLTFRALLEQAPSKRERKALLAAKVEDMINTVVR"
BASE COUNT         2492 a         4262 c         4333 g         2473 t
ORIGIN      
        1 gcgacctgca tcgcgacgcc gccgcccatg ctgtaaccga gcaaatcggc ctgcttgatt
       61 ttgaggtaat cgagcattgc ggcaatgtcg ttggcgaggt tttcataact gaaatcgcgg
      121 ttaatatcgg ccgtgcgtcc gtggccttgc atttcgacgg cgatgacttt gcggttttcg
      181 aaaattcgcc gatccactgc gtccaattgc ccggaatcgt catgaacgaa ccgtgcagca
      241 acaccaaagg ctcgcccctg ctgctaccgc tgtcgtggat ttcataatac attttgaggc
      301 cgttgatcgg cgcgtaaccg gttgttggct cttgtgcctg cgccgacacg gcggtcaaca
      361 acaggactgt gaagattaaa gctgctgttt tcatgttctt gaggtgcgcc ttgcgcgcgt
      421 cggccattct tcgtgcgtta caacgttgtg cttttgcatg ttcgatcttt aacaaatgcc
      481 ggcagggaag gtgcctccgt catgccgaat gctccagggg aaacgaatga tgctcatgcg
      541 cgactcgcca ccggccgttg tccttgcgca atccgaccgt cagccgcaac cggggcgtgt
      601 catcctgctt caattcctgc ttcgacccgc agcgcagcac ggcggtcgca aacgcaacat
      661 cgtctcccgc cgtgatgtcg agcgatacga tgtcgaacga cccatcgcct tcgcgctgcc
      721 acttgaaaaa tcccggccaa gtctgccgat aggcatcgag acccttcaca acgaccggcg
      781 gcggcacgtc gaacatcacg atgtcttcgg tgtgatgagc cagaacacct tcgatctctt
      841 tcgcccgaac ggcctttgcc caggcttcga tcaagtcgcg aattttggct tcatcagaca
      901 tttttcgact cgtgcactgc atacagggtt aggtattcac ttggcgacag gtgtcatcca
      961 gccgatgccg gcgccgccgg gctccatcag gatggcgccc ggtacatcga agatcggctt
     1021 catcagcttg gcgctggcct tgacggctga agcgacgcgc ttgtccacgt cgtccacggc
     1081 gagataggac atccagctct caggcacgcc gtcgaattcc ggcgcatcga tggaaaagat
     1141 gccggcgatc ggcttgccgc tctccatggc gcaccaatag ttgcgcccgt cgggcatttt
     1201 catcgcctcg aagcgccagc cgatcgtatc gccgtagaag cgcttcgcct gctcaacgtt
     1261 gcgcgtcatc agctcgttcc agatgaaggt cccgtgcgtc cagttcttct gctcgtccgc
     1321 gcaatcttcc tcgcgccgat gtgctgccgt ttgtgccatg tgactctcct gtcttacggt
     1381 tacgccaagt agctttcgag cttgtcgaga cagcccgtcc aaccctcgcg atggcggtcg
     1441 cgtgcgggct cgtcgaagaa ctgctcgtgg acgagcgtga ggatcgagcc ggtctcgtcg
     1501 ggcttgatca agacggtgac gagcgactcg cgctcgggca tggtgcgcca ggcccaggtg
     1561 aagacgagtt tccggttcgg caccacctcg cgatagatgc cgctcacgtg atgaacctcg
     1621 ccatcctcgg tcgagaacgt cacggcatag cggccgccca ctctgacgtc ggtgacggct
     1681 tgcgtgaccg gaccggaatc ggggccaaac cacttcagga ctttttcggc gtcggtccag
     1741 gccgcgtaga ccttttccgg cggtgcattg agacgacgtt tgagcgtgag gctgggcttg
     1801 atggcgaggc tggcttgggc gggcattgat cttcctccac gaaggcggcg agccggtcga
     1861 gccggtccga ccagaagcgc tggtagtgat tgagccagtg catggcttgc tccatcgggg
     1921 cagcggccag ctggcaggtg accgtgcggc caaccttgtt ccgtgtgatc aaaccggcat
     1981 ccgacaacac gtcgaggtgc ttcatcacgg cgggaagcga catggaaaaa ggccgagcga
     2041 gctcactcac ggaaacgcgc tcgcttcggt cgagttgcgc gagcagcgtc cgccgcgtcg
     2101 ggtcggcaag ggctgcaaag gtgcgatcga gcgtcgcttg atacttaacc attgagttaa
     2161 gtataggatc aatccggaga tgccgtcaat cgccttttgc aggaagcgag tcgaaatgcg
     2221 cgaaaatgcc cggaatcgtt gacaggatgg gccatccttc ctatgttccg cccctctttg
     2281 aagcggcccg gtcgatggct gcgccaaccc ttccacgcaa cgaatggacg ccttgccatg
     2341 aagatcaaga attcgctcag agccctgcgc aagcgccatc gcaacaaccg cgtggtgagg
     2401 cggcgcggac ggctctacgt catcaacaag accaatcgcc gctacaaggc gaggcagggc
     2461 tgatcgtcca tgcggacctc acgcggtgtc cgcttaagcc tgcgtaacgc gtcagtcttc
     2521 cgtctcgccg cgcttctctt ctgcgcgagc ttctatttcg cttcgccggc cgcgtccttc
     2581 gagagcgagc cggaaacgcc cgccgatgtc gaggaaccgg cggacgtcgc gcctgacggt
     2641 atcggagcgc cgtccataga gacatttccg agcgaggatc tgaccgagcc acgccagttc
     2701 gatcgcggcc tgttccgtcc cgacctcaag cccggagacg aaggcgcgac cgagccgcag
     2761 ccggccggcg aaagcaaaga ggacaggctc ggggctactc ccgacgattt gccgctgcca
     2821 agccccgccg agaagcccaa agtgctcggg cagctctacg tgcagctcgg caaggccaag
     2881 gacgccgaag ccgcggtgcc gatcatgacc gatatcgaga ccctctggcg cgtcaccggc
     2941 agccccaccg tcgatctcct catcggccgc gccgagctgt tcaccaaggg caaggatctc
     3001 gatctcgcgc ttcagatcct cgatgccacg gttgcaatgg cgcccgagga ggccgaggcc
     3061 tggtatctcc gcgcaaaagt ctattacttg cagagcaagt acgaccttgc ccttgccgat
     3121 ctcaagcgcg cgctcgaccg cgatccgaaa cattaccggg cgctcgaaga tctcggcctc
     3181 gtctacgatg cgttcggccg aaagccggac gcactcgagg cctatcgcaa ggcgctcgcg
     3241 gtcaacccgt tcctaaagga tgccgggctc gcggtgcagt tcctcgaaaa agaagtcgga
     3301 gcccgcaacc tctagttcac ttgctagaag tccgaatcgt tcccgacgaa ggcgatgcga
     3361 agcatgttgg tggcgccggg cgtcccgagc ggcacgccgg ccgagatgat gacgcgttgg
     3421 cccgggagcg cgaatccctc ctgataggcg atgcggcagg ccttggcgac catgtcgtcg
     3481 agatcgcggg ggtcgtcggt gagcacgcaa tgcaggcccc agacgagcgc gagcttgcgc
     3541 ccggtggcgg ggatcggcgt gagcgcgatg atgggctgct tggggcgttc gcgggccgcg
     3601 cgcaggctcg tcgcgcccga ggccgtgtag caaacgatgg ccgcgagatt gagggtttcg
     3661 gccacggtgc gggcggcagc cgagatcgcg tcggcgccgg tcgcctcggg cccgatgcgc
     3721 tgggcatgga tgatggcgtc gtagagcgga tcgcgctcca cctcgatcgc gacccgatcc
     3781 atcgtcgcca ccgcctcgac cgggtatttg ccgacagccg attcggccga gagcatcacc
     3841 gcgtcggcgc cttcgaacac ggcggtcgcg acgtcggaga cctcggccct ggtcggggcc
     3901 ggggcgaaga tcatcgattc cagcatctgc gtggccacca ccaccggctt gccggcgttg
     3961 cgcgcggcgc gggtgatctg cttctgaagg cccgggacct tctccaccgg catctccacg
     4021 ccgagatcgc cgcgcccgac catgatggca tcggcaagtt cgatgatttc ctcgagatga
     4081 tcgagtgccg acggcttctc gatcttggcc atgatcgagg accggccgca ggcgagctta
     4141 cgcgcttcgg cgatatcctc cgcgcgctgc acgaaggaga gcgcgatcca ggcgacgccg
     4201 agcgacatgg cgaagtccac atcggcgcgg tccttctcgg tcatggcgcc gaagggaagc
     4261 acggtgtcgg ggaggctgat ccccttgcgg ctgccgagga gcccgctggt catgacctcg
     4321 gcgtcgatgc ggacatcgct cgcctcgacg atgcggagct tgaacttgcc gtcatcgagt
     4381 agcagcgaat ggccgggctg cacggcctga aagatttccg gatgcggcag atggacacgg
     4441 ccgagaccgc cgaccgattc gcggcggacg aagctcagga cgtcgccctc ggtgagcttg
     4501 atcgcacccg agtcaaactt gccgatgcga agcttcggtc cctggagatc gaccagaagg
     4561 ccgatgggcc gggagaattc ggtctcgagc ttgcgaacga tggcgtgaat gtcgcccaag
     4621 ccgtcatgcg gagtgtggct catgttcacg cggaacacgt cggcgcccgc ctcgaacatg
     4681 cgccgcatcg tctcttcgtt gtttgaggca ggaccgagcg tggcgacgat cttgacgcgg
     4741 cgatttcgcc tcattgggac ttcggctcgc cctctggatc ggtcaggcgg acggtccaat
     4801 cgcgctcttc gccggtatcg acctcgaaga acccggtgcg cttgtagccg cgcttcacgc
     4861 aatccttggt gtcgcggatc gtgaacgact tgtcgtcggt gcacatatag aggcttccgc
     4921 cccattctcc gccgcgatcg taatcgacgg cgtggatgta atagaactgg gcgatcagca
     4981 cgcccttgag cagggtctcg caggcgtgcg cgggaatgtt ccaccagcct tccgtcgccc
     5041 agccttccgc gtccttgtag ccgatggcaa cgccgacccg gcttgccgtc gtgttgcaga
     5101 gcttgaggtc ggcctcagcg gtgctcgcgc cgacggtgac gcccgcggag gcgagaacga
     5161 ggccggcgag caaggtgcgc cacgagggca gggctgccga aagcgcacgc ttggcggcgg
     5221 tccggattgt ctcagaacca agcatcatcc cgactaacag cccaatatcg tggttaaagt
     5281 ggtgccgaac gttagcaccg aattggttgc gtttctctga ccagggtacc gattatagag
     5341 ccttggcgca gcggcaaaag gcccggcgaa agtcgtgaac aattgcgtcg caaccggggc
     5401 caggatcaag attttttctt gccgccgggg gagggggcag gcgagaaatt ggcgcggggt
     5461 tggcctcgcc tctcggggag cgaagtctgg acactcttgc acaagacgat ttgggccgaa
     5521 ggccctaccg cagcatcgat ggcgacgcgc gcacgggcct cctcatcctc tgcgaccatg
     5581 ccgagaacat cataccgccg gcccatgacc gtctcggcct gaaggagcag gaccttcatc
     5641 gccacatcgc ctacgatctc ggcgccgctg ccgtggccga gcgtctggcg gagatgctcg
     5701 gcgcaccggc gatcctgtcg cgtttctcgc gactcctgat cgatcccaat cgcggactcg
     5761 acgacccgac gcttgtgatg caggtcaccg atgggctggt cgtgccgggc aatgtggggc
     5821 ttgacgcgga agcgctgcaa tcgcgaatcg acacctacta cagtccgtat caccaggcga
     5881 tcgagcgtgc cgtcgattcg gccatcgggc tcggcaagcc gcccgtcatc gtgtcggtgc
     5941 acagctttac ccaagcttgg aagggtgttg cccgtccttg gacggtcggc gtcttgtggg
     6001 accaggaccc aaggctcgcg ctgcctctgc tcgccgcgct gcgggcaatt cccggcatcg
     6061 ctgtcggcga caacgctccc tattcgggac aactgaaagg cgatacgctc taccggcacg
     6121 ggacggggcg cggattggcc cacgcgctca tcgagttgcg gcaggatctg atcctcggtc
     6181 ccgaagggca ggccgagtgg gccatgcgac tggccgaggt cttgcgcacg gtgctggacg
     6241 cgggcgaggc ggcgctccat gccatcgaac tgcatggctc ctacaccgac ggagccggaa
     6301 gcacgcgccg cccgcttggc agaacggaga gaccggtcat ggacgagaag acgaagaccg
     6361 agatcgaggc ggaagccttt cggcggctgg ttcagcatct tcgcgagcgg accgacgtgc
     6421 agaatatcga tctcatggag ctcgccgggt tttgccggaa ctgcctcgcc aattggtatc
     6481 gcgatgcggc tgccgcgcgc ggtcttcctt taagcaagga ggaggcgagg gaggccgtct
     6541 acggcatgac ctacgaggaa tggaaggcga aataccagtc cgaggcgccg aagccgcttc
     6601 ggcaaagcgg ataagccgcc aggccgccat catgcgccga cgctctggat aagcgggata
     6661 aggcccccgc atgtcttgcc gcagcctatc gccgccgtaa gcttgctcta gctcgaatcg
     6721 aacaggcgag gggtcagaac cgtgcagctc cagacatcag ccaaagacca gcttcgtgcc
     6781 tttgtcgctc gcattgagcg gctcgaggag gaaaaggccg ctctcgccgc cgatttgcgc
     6841 gaggtctatg ccgaggcgaa aggcaatggc ttcgacgcca aggcgctaag atcggtcatc
     6901 cgcttgcgca agcaggacga agacaagagg cgcgaagagg aggccgtgct cgccacctat
     6961 ctccatgcgc tcggcatggc agaggcggaa gcagcctaga cgcttcacgt caaacagttt
     7021 cgaagctcag cgggtgcttt gggcaagccg cgtcggagcg gccggctcga cttcggcgta
     7081 gaggcttttc accgcctgac ccgagaaacg ctgcactgcc gccaggccct ggtagcccga
     7141 gctctggcgc agcttgaagg cggtcgggtg atccatgcct tcgaacaggt actcgacgct
     7201 tcgctgctcg ggatgcgcaa gcggtgccac ggtacggcta taggtgaccg acgcgtcggt
     7261 catgagcccc gcgatctcga agggcacgta gctcaactga tcgggatggt cctcgtcggc
     7321 ttcgggggcg ccgacgactt cggcgtcctc ataggcgggg aggcgagcag tctgcgccga
     7381 tggagccgcc tccttcggtg ccggacgcaa caggctcggg attggcaggg acatgccgcc
     7441 tacgctggca taggtgaaga ggcgccccgg aggattgggc gcacgagagt cttcgacgat
     7501 gtcggctgca ccaggcgtat aggacgcgag aatcacttgc ggcatctcga cctcggtctc
     7561 agcgtccaac tcagcctcgg caatgacatc gacgtcaggc ttggtatcag ccgaagcgac
     7621 catggtcttg ccgacgagcc ccttggccat ggcgagcttg tagtcccttg acgtgatcgg
     7681 ctttccgtcg ctcggcagat atcgcgtctt gccgtcgggg aagagagcgg cgagttcgag
     7741 ccgcgggatg cgcggccaca tgcggatctt tccggtatcg acatggacga acggaatgcc
     7801 ggaggtcggg tagtagccga caccgccgac ctcctgcaca agcgcggagt tccgcagcgt
     7861 cttcacggga acatcgggga agtggatgtc ggcggccttg ccctggatgt gctggctctt
     7921 cttcgcctgg ccgccgcctt tgcgccggag cgaagcgttg gtcgcggcgc tgcgataggc
     7981 tgaaatgaga tggatcggct tctgcgagcc gagctgcttc tgcagcgtcc agaccaggtc
     8041 gatcagcacg ggatccatct tggtttcttg attggcccgc caatcgcgca tgaaggtgtt
     8101 gatctgtttg agcgcggcct cgtcgtactt gccgtcgcgc ttgaaggtga cggtcaacgt
     8161 ctccttggtg tggatgttgt acatgctgag agtgcgcgtc tcgccggcgc tgacgcgata
     8221 cgcggtcgcc gtgcaccacg tcgccgcggc tagaaatgtc aggctcgcgc cgagcacggc
     8281 gcgacgtgcg acactccccc gaattcgcat ccccgtctta agcctttccc tcgccggcac
     8341 tcccgatgcc ggctcccgtt ggcgcgggac cccgttcagg ccacacaccc ctgcgggcgg
     8401 cccttccccg gggccgtccg ttaacctgag tattagccaa aatgcgggaa aaacaaggca
     8461 aacgggttaa gcagacctgg aatctcgggg tacggttaac aagttttccc caagttattc
     8521 acagggttag agcccaagag ccgccgccat gcgcgagtca tgaccgtaaa gatctccgta
     8581 ggttacgatc gatccatcag gattaacttt aagcgtgaaa tacgtgatgt ggacgggaat
     8641 cgtctggcgt agcgaaacct gctgatcgta cccggtctcg agcgccgaat acgtccgtgc
     8701 cgggctccag ccgaggtcgt gcttcatgat gatcgcggcc agttcccctg gattctgcac
     8761 gcgcatgcac ccgtggctct ccgcccgcac cgggtagcca aagaggtatt tttgctgcgt
     8821 gtcgtgcatg tagacgtcgt gcttgttggg gaacacgaac ttcactttgc cgagcacgtt
     8881 gccgggtccc ggcggctgat agagttcgag attgcggatg tcgacttggt cccaatcgat
     8941 cgagtcggga tcgatgtccc ggccggcata gcggatgccg agcccgtggc gctgcagtac
     9001 cgaggtgttc cacctgcccc tgcggaagaa ccaggacgcc tcctggcgca agtacggcct
     9061 gatctcatcc accttgatcg aagccggcac gtgccaatag gggccgaaca ccaccgtctg
     9121 catttcgtcg gagaaaatcg gcgtctgcgt gcgcggcttg ccgacgacga cgcgcgtcgc
     9181 gtacacgggg gcttcatcct tgacaagacg cagcatgaac tcgggaatat tcaccgtcac
     9241 gtggaacgtg ccgaggtcgc gcggaagcca gcgccagcgt tccatgttga cgagcacggt
     9301 cttgatttgc gccgggcttc cggccttatg cgaccgcttg ccgccattga ggagccgcct
     9361 cgtgcccgat ccgacggcgc cgtcgggaaa ggcgccgtga gcgagctgga agcgccgaac
     9421 ggcgtgagcg acttgcaggt cgaatctcgt ctcgatgacc ggcgttccgt cggggcgctc
     9481 cccaggcata tcgagccgct tgcggagcag cgccacgtgc tcatgctcga cgccgagctt
     9541 gagcacgggg ccgtcgggaa tcgcgactgc ggatgtggac gcgctatcct ttccgccgcg
     9601 gagctcgagc agcttttgtc tcagggcctc gaatcccggc tgatcgggct gaaagcttct
     9661 gagataggcg gcggggtcgg agcgaaaggc gatcgactcg atcacttcgg acgggttcgg
     9721 cagagcgagc gacggatcga gattgcgcga aagccgccgc ggctcgatcc ggccgccgcg
     9781 cgcgtcgcgg gcatagccga gcacggcata gctgatcttg atctcggcct cggcgagcca
     9841 tgccgcggca tcggcggcgg cggcattgaa cgtgtcgcgt gcgggcagcg cgtagtcgga
     9901 tgcgcgcaag ccatagtcgt cggccttctc gatctcggcg atcacggcct tgccgcggtc
     9961 gtttacgccg ctctcgtcga cccagagcag gcgttgttcc ggagctgcat agaactcgac
    10021 gagcgcgccc tgctcgcttt tcttcgtctc cgtcgccgtg gtgaacttgg cagcaagccg
    10081 ctgttggact gcgatgcgga tctcttcggt gcgcgtgatc agagcctcgg gcgaatcggg
    10141 ttcggcggca acaccgggcc cgctcatcga ggcggccaga agcaaagtca gcgcaacgcg
    10201 acaaatgaaa ctcatatctt gctccctcag gccgaatcgg cagggcctcg gcccaccaac
    10261 ttcaaacgtc ctccaatcgc ttcgcccaaa cccactcttg cgacatccca gcgagatttc
    10321 agttcgcgcg gacctccagt ccgatctcgc gcatcttgcg gtagagcgtc gaccggccga
    10381 tccctagcct cctggcaatc tcggtcattc gtccgcgata gcggccaatg gcaagccgga
    10441 tcatatcggc ctcgaccgct tcgagcgggc ggatgtcgcc ggtgtcgctg agcgccagga
    10501 tgccgatcgc gtccttgccg ttggcctgcg gcacattgat ggtgagcggg gttccggagg
    10561 acccgccaag catgaccggt cccgcgatgc ggggcgccgg atcgatcggc gccggtgccg
    10621 ccggtacggt ggccgcatag cccgcaacat gagcggcaat ctgcggaaac tcattcacgg
    10681 tgagcaccgg cgcgtcggtc aagacgacgg cgcggaacac ggcattctcg agctggcgga
    10741 tattgccggg ccagctgtag cgctggagca tggcgatcgc gacggcatcg atgccgtcga
    10801 tgcgcttgcc ctcctcggcg gcgaaccggg cgatgaagtg gcgaacgagc tcgggaatgt
    10861 catcgagccg catgcgcaga ggcggcaccc aaatgggcaa gacgttgaac cggtaataga
    10921 ggtcctcgcg aaagagcccc tcggtggcga gatcgatcag attgcgatcg gtcgccgcga
    10981 tgacccggac atcgaccttg accgggtgct tcgcgccgac cgcatcgatc tccccgtctt
    11041 gcagggcgcc gagcaacttt gcttgcgcgt cgcgcgtgag ctccccaatc tcatcgagga
    11101 agagcgtgcc ctggtcggct tcgacgaatt tgccgatgcg cttctccggg gcgccggtca
    11161 gcgccccctt ctcgaggccg aacaggatgc tctcgacgag atgctcgggg atcgcgccgc
    11221 aattcacgct gacgaacggc ccagatttgc gggcgctttc gccgtgaacg gcacgggcga
    11281 ggagttcctt gccgacgccc gactcaccct cgatcagaac gggaatagtg gaatcggctg
    11341 cgcgcctccc gagctccaag acccgggtca tcacgtcgct gcccgcgatc agatcggcga
    11401 aaccaagcgc acacctggcc gaggtcttga tgcggaccag ctcgtcggtc agcgcctcga
    11461 ttttgagtgc gttcttgatc gagacctcga gccgctccgg agtcacgggc ttcaccacga
    11521 agtcgacggc gccggcccgc atcgccgcga tggcggcagc gatgccgccg tggctcgtct
    11581 gcacgatggc gggaggcttg cgcggcaaga cgcgcatggc ggaaagaacc gcaagcccgt
    11641 cggtgccggg catgaacagg tcgagcagga caagaccgat atcgcccgcg gtgtcggcgt
    11701 tgagagcggc gagcgcttcg tcgccgctgt cagccgtggc gacgggatag ccgaaccgct
    11761 cgatggcttg ttcgaggata cgacgctggg aagggtcgtc gtcgacgacc aggacgcgtt
    11821 ttggcatgac gcaggactca aactcgacgc tttgaccgaa cttcaggagg tgaggccacg
    11881 tcggcgcgag tgacaccggg gcgaaaccat ctcctttgtg ccaaatttga gcggtggaag
    11941 gtaaatatcc cctttatctt ggttcgattt tggtgagatc gccggcgctt actccgcgaa
    12001 aaggtccaaa acgcatggtc tttgatcctt gccgcagcgc gctatccttc ccacactaga
    12061 ctaaaccgac gctttcacga ggtttacgcc accgatgccg ttcaaagtcc gtaactggcc
    12121 agagcacccc gcatttgccg cgaccgcctc cgcaaggggc aaggcgaaag ccgagacgca
    12181 atccgaagct gaagggacgt cgaacctcgg cgcgctgccc gaatggaagc ttgccgatct
    12241 ctacgagaga ccgaacgcgc ccaagctcga ggcagatctt gcggctgccg aacgcgaggc
    12301 caacgcgatg caggatcgct atgcgggcaa gctcgccaag ctgctggatg gcggcaaagg
    12361 cggagctgct ctggccgagg ccgtgcgcac gttcgaggcc ctcaacgatc tgatgggccg
    12421 gatcgtctcc tatgccagcc tgctctacgc cgccgatacg tcagacccca agcgtcagaa
    12481 attcttcggc gatatccagg agaagatcac cgccatctcc tccaagctcc tgttctttcc
    12541 gctcgagctc aaccggctcg acgacgccaa gctcgaaacg gcgatgtcga agtcagtgct
    12601 cggccattac cggccctggc tcgaggattt gcgtaaggag aagccttatc agctcgagga
    12661 caagctcgag caactgttcc acgagaaggc cgtgacggcg cgcggcgcct gggaccgcct
    12721 cttcaacgag acgatgacgt cgctcagatt cgagaccgac ggggagaagc tcagcctcga
    12781 gcccacgctc aaccggctcc tcgatcaaga cgaagcaaag cgccgcgacg cggccgaaag
    12841 cttggccaaa gtcttcaagg agaatgtgcg gctgttcacc ctgatcacca atacgctggc
    12901 gaaggacaag gagatttccg accgctggcg cggctttgcc gacgtcgccg attcacggca
    12961 cctcaacaat cgggtcgaga aggaagttgt cgacacgctc gtcgcggccg tgcgcgacgc
    13021 ctatccgcgc atctcgcacc gctactacgc gatgaaggcg aagtggctcg gcaaggaggc
    13081 gctcgcacat tgggaccgga acgcgccgct ccccgaggag ccgaagcagg tgatctcctg
    13141 ggccgacgcg caaaagacgg tactcggcgc ttaccgcaat ttctcgccgc gcatggccga
    13201 catcgccgga gagtttttcg ccaagggctg gatcgacgcg ccggtgcggg aaggaaagtc
    13261 gcccggcgct ttctctcatc caaccgtgcc gagcgcgcac ccctacatcc tgctcaacta
    13321 tcagggcaag ccgcgcgacg tgatgacgct cgcgcacgag ctcggccacg gcgtccacca
    13381 ggtgctcgcc aacgtgcagg gtccgctgat ggcgccgacc ccgctcacgc tcgccgagac
    13441 cgcgagcgtg ttcggcgaaa tgctgacgtt tcgcgccctg ctcgagcaag cgccgagcaa
    13501 gcgcgagcgc aaggcgctgc tcgcggccaa ggtcgaggac atgatcaaca cggtggtccg
//
LOCUS       DASUQP010000023        32702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1646438, whole genome shotgun sequence.
ACCESSION   DASUQP010000023 DASUQP010000000
VERSION     DASUQP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 32702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32702
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646438"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..155
                     /locus_tag="VFY74_01070"
                     /pseudo
     CDS             1..155
                     /locus_tag="VFY74_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
     gene            181..423
                     /locus_tag="VFY74_01075"
     CDS             181..423
                     /locus_tag="VFY74_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003607513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX5930333.1"
                     /translation="MHVVGAVLEPIPVGSMDIIMPQRSISGSPNGSPVTMATMLEFAA
                     RHDISPQTEHYQMDQVNEALAHLEGGKARYRIVLDA"
     gene            858..1028
                     /locus_tag="VFY74_01080"
     CDS             858..1028
                     /locus_tag="VFY74_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930334.1"
                     /translation="MLLTADQHRRLAEVYEEEAADQSHKPEDRALLAEKAARFRVLAR
                     MAEEKAKERRLN"
     gene            complement(1368..1444)
                     /locus_tag="VFY74_01085"
     tRNA            complement(1368..1444)
                     /locus_tag="VFY74_01085"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1408..1410),aa:Ile,seq:cat)
     gene            1656..2378
                     /locus_tag="VFY74_01090"
     CDS             1656..2378
                     /locus_tag="VFY74_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5930335.1"
                     /translation="MAHSTQNLLALLLSAATLGAPAAAHDQPEKRTISLSASGIVKTT
                     PDMVEISTGVTSEAPSAREALDKNTASMTKVVETLKGEGIDAKDIQTANFSVGPVYEQ
                     RPYDGPPENKPPVIVGYRVTNQVRITLHDTAKLGAILDKVVSLGANQIDSIEFGVEEP
                     EALKDEARKLALKNVTDNAKLYAEAAGVGLGPILSIAEEESSYQPRYAAAAARMETAK
                     DVPIEAGTASVEVRVRVSWEIN"
     gene            complement(2668..3084)
                     /locus_tag="VFY74_01095"
     CDS             complement(2668..3084)
                     /locus_tag="VFY74_01095"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5930336.1"
                     /translation="MFRRRGTIIAEGLKIVGSVTAEGLVEVNGQIEGDLYCTALVVSP
                     KASILGGIQAERVVVNGRVEGPIRGGDVVLKSRAHVVGDIQHETLSIDSGAYFDGRSV
                     RTGQVNGQAREHAQGEKVSARPRKEAPQNGREVAAA"
     gene            complement(3334..3567)
                     /locus_tag="VFY74_01100"
     CDS             complement(3334..3567)
                     /locus_tag="VFY74_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930337.1"
                     /translation="MRRVCLEGSRAAFAARFGVWPGSGRIKALCGNEGFSVEAMRLAM
                     RSEAAIDTAATTIAAIHVAGLIVNGRILATRRI"
     gene            3724..4068
                     /locus_tag="VFY74_01105"
                     /pseudo
     CDS             3724..4068
                     /locus_tag="VFY74_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(4312..6336)
                     /gene="rpoD"
                     /locus_tag="VFY74_01110"
     CDS             complement(4312..6336)
                     /gene="rpoD"
                     /locus_tag="VFY74_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX5930338.1"
                     /translation="MATKAAEAEDQEQTQAEPADGPVLDMSDAAVKKLIKTAKARGFV
                     TYDELNEVLPSEEVSSEQIEDTMSMLSDMGINVIDTDEAEETPQQGEEGGESPRAVVA
                     PQPVAKAETTKEPSERTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREAMIAGLC
                     ESPLTFQAIIIWRDELNEGKILLRDIIDLEATYQGPDAKPAPSQPLPGEPGHGETNGN
                     GQAAPGVAGARAGADEESEPEPEEFEDDEDDLENALSLAAMEAELKPKVLETFDLVAT
                     NYKKLRKLQDQQVERALKNDSLSSGQEKRYKLLKDEIVAEIKSLSLNANRIESLVETL
                     YDINKKLLGYEGRLLRLAESYAVSREDFIGEYQGNELDPNWLRRVGRLKKKGWIDFIR
                     DERGLVKELRSEIQNLAAETGLQISEFRRIVHIVQKGEREARQAKKEMVEANLRLVIS
                     IAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQA
                     RTIRIPVHMIETINKIVRTSRQMLHEIGREPTPEELSEKLAMPLEKVRKVLKIAKEPI
                     SLETPIGDEEDSYLGDFIEDKNAVLPIDAAIQSNLRETTTRVLASLTPREERVLRMRF
                     GIGMNTDHTLEEVGQQFSVTRERSRQIEAKALRTLKHPSRSRKLRSFLDN"
     gene            complement(6849..8753)
                     /gene="dnaG"
                     /locus_tag="VFY74_01115"
     CDS             complement(6849..8753)
                     /gene="dnaG"
                     /locus_tag="VFY74_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5930339.1"
                     /translation="MKFSDSFLDEIRARLPVSQVVSRRVPLKRAGREWKGLSPFNKEK
                     TPSFTVNDQKGFYHDFSSGKHGDIFTFVMETEGLSFPEAVERLAGEAGLELPKPDPQF
                     ERTAKERLGLFDALEAAARIFEEALQAREGRVAFAYAEGRGLSRDTLKEFRIGFAPNA
                     KDALKPALLKKGFSEAQLIEAGLLIKPEDGRATYDRFRNRLTIPILDAKSRVIAFGAR
                     ALDPEAEPKYLNSPETRLFDKGSTVFNFARARKVAFDIGELIVVEGYMDVIALHQSGF
                     ANAVATLGTAFTERQMELLWQLAAEPVVCFDGDRAGEAAAARAIDRMLTNLREGRSFR
                     FAFLPEGSDPDDLVRTKGASALSACVASARPLIDVLWQRELKLHAVDTPERRAAFEAR
                     LETLLAEIANARVKEHYRREVKNRLFALWREKGFRGKPPANAPRSAGRAGPPPNPSPD
                     GFATVIVLALVNHPWLLDRFAEEVSALDIRDRKLASLLRTVIGIILEDGHLTDERVAE
                     RVAASSHGKVFAKLMRESAFTRLAFLDRQSPKEEVEAQFADLIYRFRALPNLTRELEQ
                     SADRLADVTDAEFDRFAALQQQVASIGNQHTADDAGDRDAAKRFEETIARLKGANIGA
                     QRGRRPEKQS"
     gene            9306..9539
                     /locus_tag="VFY74_01120"
     CDS             9306..9539
                     /locus_tag="VFY74_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX5930340.1"
                     /translation="MTEEEIEAAARADPEWAGLLDIDWSGAKFVMPRRKEAISIRLDE
                     DVLAYFKSLGTGYQTRINAVLRHFVEQTRARRK"
     gene            complement(9639..10052)
                     /locus_tag="VFY74_01125"
     CDS             complement(9639..10052)
                     /locus_tag="VFY74_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930341.1"
                     /translation="MAKFSHSRFRAAVSALLALALTAAGAPVLAAEPPTHNLGPVGPR
                     EPILVTVGTQRVIAFFVPERGGCALSAVTWKEGDASAPYASARVRVSLKPGQTLALDG
                     AQRQSMSLLCGADAATLAVVAPAELIFPGPPNGAK"
     gene            complement(10444..10893)
                     /locus_tag="VFY74_01130"
     CDS             complement(10444..10893)
                     /locus_tag="VFY74_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004688577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5930342.1"
                     /translation="MRETITAALKHATKAQDKRRISTLRLMSAAIKDRDIAARTAGKT
                     QATDAELLELFAKMIKQREESEKIYAEAGRAELAKQEAEEIVIIREFLPQQLSGEELD
                     KAVADAIAATGASSAKDMGKVMAALKARYAGQMDFARASALVKTKLG"
     gene            11148..12395
                     /gene="carA"
                     /locus_tag="VFY74_01135"
     CDS             11148..12395
                     /gene="carA"
                     /locus_tag="VFY74_01135"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5930343.1"
                     /translation="MTDTRETLAAEAQPPRARAEAGWTDETPTGLLVLADGTLIEGKG
                     AGAAGIAVGEVCFNTAMTGYQEILTDPSYAGQIITFTFPHIGNVGTNSEDMEAGTLAA
                     GGVRGCVLRAPITPASNWRAAQGLDAWLKARGIIAVTGVDTRALTALIREKGMPNAVI
                     AHAPDGKFDREALKREAAAFPGMVGLDLAKDVTSGQSYGWDETAWQWGKGYGRQESPR
                     FHVVAIDYGLKRNILRELATVGCRLTVVPATTTAEEILERKPDGVFLSNGPGDPAATG
                     VYAVPEIKKLVASGLPVFGICLGHQMLGLALGGTTAKMHQGHHGANHPVKDFTTEKVE
                     ITSMNHGFAVDRDSLPKSVEETHVSLFDGSNCGLKVKDRPVFSVQYHPEASPGPQDSH
                     YLFTRFVAMMEKRKGADKGEREQ"
     gene            complement(12461..12808)
                     /locus_tag="VFY74_01140"
     CDS             complement(12461..12808)
                     /locus_tag="VFY74_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX5930344.1"
                     /translation="MKQIGTHRARSLRADLTDAERKLWYQLRDRRLAGWKFRRQVPVG
                     PWIVDFLCKEKGVVVEFDGGQHNASERDRRRDAALARQGYRVLRFWNSEVLQNQEGVL
                     TIILSVLESETAE"
     gene            12867..16304
                     /gene="carB"
                     /locus_tag="VFY74_01145"
     CDS             12867..16304
                     /gene="carB"
                     /locus_tag="VFY74_01145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5930345.1"
                     /translation="MPKRTDIKSILIVGAGPIVIGQACEFDYSGTQACKALKQEGYRI
                     VLVNSNPATIMTDPELADATYIEPITPEFLTKIIEKERPDALLPTMGGQTALNAALSL
                     DRGGVLKRYGVEMIGARAEVIDKAEDRELFRESMHRIGLETPRSRLADAATLKRADRQ
                     KNKDAIARIETSHLDPEERTRALSGFEAEWARGESERRRRYVEKGLIEALDALPDIGL
                     PAIIRPSFTLGGTGGGIAYNREEFLEIVERGLDASPTCEVLIEESVLGWKEFEMEVVR
                     DKDDNCIIVCSIENIDPMGVHTGDSITVAPALTLSDKEYQVMRNASIAVLREIGVETG
                     GSNVQFAVNPADGRLVIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELAND
                     ITGGATPASFEPTIDYVVTKIPRFAFEKFPGAQPVLTTSMKSVGEAMAIGRTFPESLQ
                     KAVRSMETGLTGLDDITFEGLGQGDDKNVIRAALGTPSPDRLLKVAQALRLGFEEAQI
                     FEACKIDPWFIRQIKDIVDMEARVREHGLPANPGQFRALKAMGFSDERLGKLAGVEAS
                     EVSAMRRTLDVHPVYKRIDTCAAEFASPTAYMYSTYEAPFAGRAECEARPTQAEKIII
                     LGGGPNRIGQGIEFDYCCCHAAFALSDAGYETIMVNCNPETVSTDYDTSDRLYFEPLT
                     EEDVLEIVRTERQNGTVLGVIVQFGGQTPLKLAQALERAQVPILGTSPDAIDLAEDRD
                     RFKALIERLELRQPRSGIARSAKEAKAIAAEIGFPLVIRPSYVLGGRAMEIVHDEAQL
                     DRYIAEAVVVSGKSPVLLDSYLRDAIEVDVDAIGDDKDVFICGVMEHIEEAGVHSGDS
                     ACSLPPHSLKPEIVADLERQTIELARALNVVGLMNVQFAIQHDDIYILEVNPRASRTV
                     PFVAKVIGLPVAGIAAEVMAGKKLADFGLKRPKLRHVAVKEAVFPFARFPGVDPVLGP
                     EMRSTGEVMGIDRNYAVAFAKSQLGGGSRLPLSGTVFVSVKDADKDKVLAPVRALAEM
                     GFRIIATRGTKRHFDAHGIACERVNKVLEGRPHVVDAITNGEVDIVFNTTEGAKALAD
                     SFSIRRSALLYHIPYYTTVAGALAVTEAIKALRAGSLNVAPLQSYVGTRPYNLAETA"
     gene            16394..16867
                     /gene="greA"
                     /locus_tag="VFY74_01150"
     CDS             16394..16867
                     /gene="greA"
                     /locus_tag="VFY74_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5930346.1"
                     /translation="MEKVPMTAEGYAALEAEIKYLKTVERPRIIKQIADARTHGDLSE
                     NAEYHAAKEQQGITEARVADLEDKLSRADIIDVSKLKGDQVMFGATVTLLDEDTDGKV
                     KYRIVGELEAEVRSGKISITSPIARALLGKRKKDIVEVSTPGGGKSYQVLKVEYK"
     gene            16960..17439
                     /locus_tag="VFY74_01155"
     CDS             16960..17439
                     /locus_tag="VFY74_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX5930347.1"
                     /translation="MSCLTVRAIALACVIFFAAGMASAETVGSITVENAWSRTVPSGS
                     SVAAGYLSIRNDGDEPDRLLSASASFASATEIHQTTMSDGVMQMRPVADGVIIPAKST
                     VMLEPGSYHLMFRQLTRPIKEGDTLPATLAFERAGNADITIQVLGIGAQGPKAEPRQ"
     gene            17564..18031
                     /locus_tag="VFY74_01160"
     CDS             17564..18031
                     /locus_tag="VFY74_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5930348.1"
                     /translation="MISRPLALTLLACIASATLALAHEGATGVVKERMDLMESQKEAM
                     KVIGDMARGKKKFDAAAATKAANDISSTAKKIPELFPEGSGGGKSDALPAVWEKWDRF
                     TDNAEDLATNADALVKALADSASAAWKPAFQKVGDTCKSCHEDFRAEKKEKHH"
     gene            complement(18300..19289)
                     /locus_tag="VFY74_01165"
     CDS             complement(18300..19289)
                     /locus_tag="VFY74_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HEX5930349.1"
                     /translation="MTRTSLAAVLSAALILSAAPSFAATIFVANEKDNTITVLNGETL
                     ETIKTVKVARRPRGIILSPDFKELFVAAGDGDIMDVIDTKTLEVTRQLESGPDPELMA
                     VDSKGEIIYIANEDDSLVTIMDIKSGEVLGEVPVGVEPEGMTVSPDDRYTAATSESTS
                     MAHIIDNKTRTLVGNILVDSRPREAKFTKDKKELWVSSEIGGTISIVDTSNWDDPKSW
                     KVVKKIGFEVEGVRPEQLQPVGMDFSLDGKLMFVPLGPSNRVAVIDTATKEVKEYILV
                     GQRPWHGELSPDGKKYYVANGLTNDITIIDVESLKAEKSVPVGRLPWGVAVMP"
     gene            complement(19311..20669)
                     /locus_tag="VFY74_01170"
     CDS             complement(19311..20669)
                     /locus_tag="VFY74_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5930350.1"
                     /translation="MLRSPFDRFRVCRKILASLVALALFVPAVVASAHAAAPDPPEPA
                     TVPAPAAEAPSAPAGTQAPAAPAAATPALPGSSAAKNMRIVYLGKEYPEPLPLSYAEK
                     PIIDKGIQGALLMLKEANQAGTFVGHSFELIEAIVPEDGDVVAKAKELLAAGERFIIA
                     DLEPADLLAVADLPDAKDAVIMNIRSSATALRQEQCRVNVFHVIPDYAMRADALAQYL
                     IWKKWPRWFVIRRDTPEDQDYLAQVKRSAARFGGKVVTDKVYALPPGARNLDSGHQQI
                     QAQIPMETQEASEHDVVWVLNSDDDFGDYLMYRAYLPRPVVGTQGLQATAWDKSYTES
                     GGMHFQNAIPRIAKRTPVERDYTAWLGFRALADSAMKSGKTAPQDVKAFMLSDAFKLE
                     GFKGQAMSFRTWDHQMRQPIILGGGTRVPVSTSPQEGFLHPTNLTDTLGFDAPETKCK
                     FK"
     gene            complement(20982..21653)
                     /locus_tag="VFY74_01175"
     CDS             complement(20982..21653)
                     /locus_tag="VFY74_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5930351.1"
                     /translation="MHKIITGLFTLVIIGGMETFAWKAIAADAPAEQAAPAQPAAPAQ
                     PTGQAAPAQPAAPAQPTGEAAPAQPAAPAEGAAASAGGGECCKAGDTTPPLDLIKATP
                     KGGLHNPYNDKIAEMAEEGHKKYLSFSCNGCHGGGGGGGMCIPLTNDTWVYGPDDDVL
                     FRLIALGSVDLTKAGYKRVKSETVVGPMPPFGGIIKTSDDLWKVIAWMRSVAPPGSEK
                     KPPLQ"
     gene            complement(21804..22634)
                     /locus_tag="VFY74_01180"
     CDS             complement(21804..22634)
                     /locus_tag="VFY74_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930352.1"
                     /translation="MTMQGLSRWLLVARALALASALGFLVLGPASAAEVDESTVKQAV
                     ADVIAERSKDGVFVFHDPKLDADLNLVFEQVKIVRGMEGYGWFANTIFHDKDVPKKQY
                     AIDFWFKPEGDKLKLMDIRVQKGPKPEGDGWIMVTRLPVAWWWLPVQEHPGDMEVTRG
                     WHVMSAIHNYIAANKDENGNLLVKDDKSGETVPLEFIEMHQPIRRLKEDGRYFACTDF
                     RKPGSKDEYYDIDFWLETKSGKLRVADVKMHKVPVQEDGIWTQVPRYTFEGMDFDVTN
                     "
     gene            complement(22631..24454)
                     /locus_tag="VFY74_01185"
     CDS             complement(22631..24454)
                     /locus_tag="VFY74_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HEX5930353.1"
                     /translation="MSLRILTAIVSALALVLVSVPAEAAKKQVKRDLSKEFDDLTPSE
                     QIAIRAAAKAAYKGKKLESLMVCADPGNMPLSNIKEEGFQNKLATLLGKAMGARVVYS
                     WRPFIERGLTRQTFDQDMCDVMFDMPANYGRLLTTFPVYKTPYVLVYRNDKGLELTGL
                     DDPKLKDVKIGVFQTSGIRAALARRGIVDNVKLQTQTHDGDLVIENQPWYPVQKVVDG
                     DLDVAAVFGPFAGWVKTMKGEPITILPVNLQEDKVPLEFEMAIGVRKTDAFLKYMLEF
                     ALEDHAEEVEKILRDYGVPLVQCSKCIVAGDLPAHGSYIAFADQKFEARPDLASPDQV
                     VTKDKLETWIAEGADLNQELSNAINANDIERVKFLVEKGAELNKPDPQGWLPLNNAAR
                     QRKDGMIKTLIELGADPNLADEGGTTPLVAAAMRDHVPSIKVLLENGADVDKPGPGGF
                     HPLALAIAEARYEVAKALIEGGADVSAASGGDGLTPLMLIASQTSPAEGSRFLPGSAH
                     PTDIAKALIERGAEVNAQAKNGMTALMIAATHNSAPMIGLLMDAEADPTLKNAQGLTA
                     TDVAEKNGNLEAAQAILVLGAGRSAAAPSPADNAAQTTTSQ"
     gene            complement(24593..26479)
                     /locus_tag="VFY74_01190"
     CDS             complement(24593..26479)
                     /locus_tag="VFY74_01190"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX5930354.1"
                     /translation="MKMKFGVLAGTVIAVMMGLSGQAVADSDAEQLARMKDPDQWPAP
                     GRDFSLTRHSTLADINTKNIGKLQMAWLQGTNALRGHEGQPLVIKDVGGKTMLFMVSG
                     CPAMSNCNVVQALDLTDPDNPKAVWSYVKKTDRDESAVPRACCDTVNRGGSYADGKFV
                     YGTLDGFVIALDGQTGKEVWVVKYAYPEKGETITPAPLIAADKVIMGFGGDEFAARGR
                     VAAFNLKDGSEAWVCHSTGSDKDVCLTADTNKANPHFGTAGQDLGIKTFPGEDYKIGG
                     GAAWGWFSYDPELKMVYYSTGNPGLWSPSYRCPDKTHEECNTGDYDNKWSMTIFGRKV
                     DNGEAVFAYQMTPFDQWDYDGVNENMLTDMDVDGKKRKVLSHFDRNGFAYVLDRADGT
                     LLRATKYVTTDWAEKVDMKTGRPIKVRDKSPLEVGRNVSSCPSAMGGKDQQPGSVDPK
                     EPNIFYMPTNNWCMELEPQERTHTNQGTVYVFANVYMYPEKQGTTGKIKKYDVLTGKT
                     LWEIPDPYPNWGGTMVTDGGLAFYGSLGGDFRAVDRSSGKILWSRKLGSGIIGNPITY
                     KVGGKQYVGVYSGIGGWIGLPVTAGLDLNDKFGAIGATAMTKAANLDKIPQGGELNVF
                     RIYE"
     gene            complement(27124..28626)
                     /locus_tag="VFY74_01195"
     CDS             complement(27124..28626)
                     /locus_tag="VFY74_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930355.1"
                     /translation="MTVASPARNTEARRTLLDKLKGSLLTPIRAMRLRYLPLLMIYYA
                     YGALGLIAIAQTFWVKEALTFTPAELAALAVWLTLPWTVKMVFGQLADSVPILGSQRR
                     VYVFIGAGLVASGMIILAGAAGGWITLASKNALYVTAQLMIVIGIVLQDVIADAMTTE
                     VVERVRPDGTPRPKAEVDRELGLVQVLGRLALWSGILSVGGLSGWLAQVYSYETVFLL
                     GLAVPAISLTGAMLVHLDGTAPKPIDWRILGGGLLFGAAVVAIGLSGLPLGQELVFAI
                     SLAVISLMLARIAGDLDEVHRQRIFYAALVIFLYRASPGVGEGYRWFTIDVLGFDEAF
                     YGTLAQIGAALSLAGAWLFSDVITRKPVAQVLLWLTIIGTVLSLPTIGLALRLDQWTE
                     ATFGFGARTIALVDTTASAPFAELSMIPALTIVAINAPAGKRATWFALMASLMNIALV
                     AGQLQTKYLNLLFAVDRGSYANLAALVVAAVTIGFVVPVAGIIAFGKRAT"
     gene            complement(28666..31854)
                     /locus_tag="VFY74_01200"
     CDS             complement(28666..31854)
                     /locus_tag="VFY74_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX5930356.1"
                     /translation="MIAAIIRWSARNVLLVGLATVFVTALGIHAVSRVPLDAIPDLSD
                     AQVIVYTEFPGQAPQVVEDQVTYPLTTAMLTVPKSRVVRGFSFFGVSFVYVIFEDGTD
                     VYWARSRVLEYLSSAARRLPQGVTPSLGPDATGVGWVYQYVVRGAQRSLAELRTLQDW
                     YIRFGLSNASGVAEVASVGGFVKQYSVVIDPRRLQALGIPLSRIRDVIRASNMDVGGR
                     TVEVSETEFAVRGRGYIKSLSDLEQIVVKNEGGVPVQLKDVARVELTADERRGIAELD
                     GEGEVVSGIALQRFGENALSVIQNVKQRLAEIAPSLPEGVTVEAVYDRSALIYRAIDT
                     LKRTLIEESLIVALVCVIFLLHARSALVAIITLPLGILIAYICMWAMGLSSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKRLERAPPGKKRHEIVVEAAIEVGPALFFSLLIITVSF
                     LPIFALEAQEGRLFKPLAYTKTFAMAAAALLSILLVPALMTLFVRGRIIPENKNPVNR
                     FLIWIYRPAIELALKFKGATIAIALAVLALSLWPASRLGSEFMPNLNEGTLMYMPVTL
                     PGISVTKTAELLQTQDKIIKSFPEVESVFGKAGRAATATDPAPTEMVETVINLKPESE
                     WRAGLTVDKLIAEMDRSLQFPGVSNAWTMPIKARIDMLSTGIRTPVGVKVFGKDLGEM
                     EKLARKIEAVLRDVPGTASAYAERAISGYYLNVDPDRAQLARYGLMVGDVQDVIAMAL
                     GAEPVTTTVEGRERYTVSIRYPRDYRSRPQDIASEVLIPLPGGGTVPLGEVAKVSLAQ
                     GPSSIRTEDAQLALYIFVDLRDRDLGGYVAEAKKAVAEGVDFPPGYYVTWSGQFEYFE
                     RAKARLQIVVPVTLLVIFLLLYLNFRRITETLIVMLSVPFALVGGFWLMWWLGFNLSV
                     AVAVGFIALAGVAAETGVVMLIYLDHALKDLRKQREREGRAFTGKDLHAAIMSGAVER
                     VRPKIMTVAAIMAGLLPIMWSHGTGAEVMQRIAVPMIGGMVSSTLLTLIVIPAIYAVV
                     KGWSLRRERAATVDAPRSRRTRPALAGDTPAS"
     gene            complement(31851..>32702)
                     /locus_tag="VFY74_01205"
     CDS             complement(31851..>32702)
                     /locus_tag="VFY74_01205"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5930357.1"
                     /translation="YVEDLFVNKTGQHVREGEPLFRFYSPQIQLAQADLVVAMRAEGA
                     SAKSDTSRNVAGAIQRLRNLGVPDSRIDEVRNSLTNPRTIDWASPATGDVIEKKVIEG
                     QRVAAGDELFRIADHANVWVVAEVAEADIAAIKVGSPATVTLRASAGEPHEGVVSFIY
                     PEMMKPETRTISVRIELPNPDGNIRTGMYADVLFRAGEGDAPATAIPDSAIIDSGTRQ
                     IVLVAKGEGRFEPREVKLGRRGEGYTEVTSGLELGEEVVTSATFLIDAESNLKAALQA
                     FSQGAQP"
BASE COUNT         6053 a        10168 c        10372 g         6109 t
ORIGIN      
        1 gcgcagaggt ggtgtcgctt caccaaggcg atgcagtcgt gttcgctgtg catcaccggc
       61 cggtagatgg cgcgcgcggc acctatcggg tgaacctgcg tcacggcgtc agccgcgtga
      121 ggtctgggcg ccgacacacg cttgggatca tcttccagca cgctacggcc caagggacgc
      181 atgcatgtgg taggcgcggt gctggaaccg atacctgttg gctccatgga catcatcatg
      241 ccgcagcgaa gcatctcggg ctccccaaat ggttcgcccg tgaccatggc aaccatgctg
      301 gaattcgcgg cccggcacga catctccccg caaactgaac actaccagat ggatcaagtg
      361 aacgaggcgc tggcgcattt ggaggggggc aaagcgcgtt atcgcatcgt gcttgatgca
      421 tgaggctcaa taactggcag gagttcctgt caggtccgca tctgatgctg tggacggctc
      481 ctccaccggc acatgagtgc catggatgtg ggtgctgtta gaggtcccca cacatggcac
      541 ttagcggacc tgagcgaggc tcccgcacgt ccgctattgt ggcaaagcag acattccccg
      601 ctacgacctc aaacaatgcc gatttatgag tacacgccct aaggcggcgc tcgcttcgat
      661 cgcggccggt cccgattagg cctgtggaga ctaacgcgcc ccttgttgac aggaatcaag
      721 acaggcgtgt cttctggtgg gcatcatcat ggaggggaac catggacaac accacactgc
      781 taatcgtcat tttggtgatt ttacttttgt ttggtagcgg ttattacggg cggggccgct
      841 ggtggtgaga ttcaccgatg ctactcacgg ccgaccagca ccgccgcctt gccgaggtct
      901 acgaggagga ggcggccgac caaagtcaca agccggaaga tagggcacta cttgccgaaa
      961 aggctgcgcg gtttcgagtg ctcgcgcgca tggcggaaga aaaggcgaag gagcgtcgtc
     1021 tgaactaaca gcgcgtctcg cgctcgtccg gcgctgaggt gccgaccgcg cgcacggcat
     1081 cctcaaggtc ctgctcggac acgtcagcgc tgcggtacga gatagcgggg agctagaacc
     1141 ggcgatgttg aacgacttgt ctcaaatcgg cgggtgcacc ccggggtgct cgcggatccc
     1201 gtcggactaa tcccttctag gcgacctcag ggactcgctg gcgggtttct tcttcccgag
     1261 tctctcccag atgttgaacg ccagcggaac atctattccc gtaccttcgc atccggcgcg
     1321 ggccaaaggg gaaaaacgca aatgaaatca actgctgttt ttgaaagtgg tgggcccggg
     1381 agggctcgaa cctccaacca gacggttatg agccgccggc tctaaccagt tgagctacgg
     1441 gccccttcgc cgacatccct ttcggccaga atgccgcacg ggtctttgtc tctgacgcat
     1501 catcttgccc gaaagtctgc aacttttcgg gatgatgctg gcggatttcc gcctcatagc
     1561 agagttcgcc ggcaagcgcg aattgctgca agttcatttg cggttcatat ggcgccgggt
     1621 catgctgatg aaagccccat agcgtgagaa cccccatggc tcactccaca caaaacctcc
     1681 tcgccctcct cctctccgca gccaccctgg gggcgccggc cgcggcccac gatcagcccg
     1741 agaagcggac gatttcgctg agcgcgtcgg gcatcgtcaa aaccacgccg gacatggtgg
     1801 agatctcgac cggggtcacc agcgaggcgc cgtccgcgcg cgaagctctc gacaagaaca
     1861 ccgcgtccat gaccaaggtc gtcgagacgc tgaagggcga aggcatcgac gcgaaggaca
     1921 tccagacggc gaatttctcg gtcgggcccg tctacgagca gaggccttac gatgggcctc
     1981 ctgagaacaa accgccggtg atcgtcggct accgcgtgac caatcaggtt cgcatcacgc
     2041 tccacgatac ggcaaagctc ggcgccatcc tcgacaaggt ggtgagcctc ggcgcgaacc
     2101 agatcgactc catcgagttc ggcgtcgaag agcccgaggc gctcaaggac gaggcgcgca
     2161 agctcgcgct caagaacgtc accgacaacg ccaagctcta tgccgaggcg gccggcgtcg
     2221 ggctcgggcc gatcctcagc atcgccgagg aggaaagctc ctatcagccc cgttacgcgg
     2281 cggcggctgc gcggatggaa acggcaaaag acgtgccgat cgaggccggc accgcttccg
     2341 tcgaggtacg cgtcagggtg agctgggaaa tcaattgacc tcgccgtcgt tccggcgcaa
     2401 gccggaatcc agagccgcta gcacggcgcg cgtgcggcgc tttcaacagc atcccctctc
     2461 cccttgaggg ggcgagggct tcccgaccgg cgcgcgaagc gcgacgaatg gcgggaaggg
     2521 tgagggctga tcccttttga cgcaggctta cgccgcttac ccctcatcac gccacgagcg
     2581 gcgcgtgctc tcccccaaga aggggagagc ggacaaactg gataccggct ttcgccggta
     2641 tgacgagtct cgtgtgacga gtctcgccta agcggccgcg acctcgcggc cgttttgagg
     2701 ggcctctttg cgcggccgcg cggagacctt ctctccttgc gcgtgctccc gcgcctgacc
     2761 gttcacctga ccggtacgca ccgagcgccc gtcgaaatag gcgccggagt cgatcgaaag
     2821 cgtctcgtgc tggatgtcgc cgacgacgtg cgcgcggctc ttgagcacga catcgccgcc
     2881 gcgaatgggc ccttcgaccc gtccattcac cacgacgcgc tcggcctgga tgccgcccaa
     2941 gatcgaagcc ttgggcgaga cgacgagagc ggtgcagtaa agatccccct cgatctgccc
     3001 gttgacctcg acaaggccct cggcggtaac cgaacccacg atcttcaagc cttcggcaat
     3061 gatagtccct ctacgcctga acatgacgtc cctcctacgc gaacaacggt tgccatgaga
     3121 gcctacgcca acactcgtgc ccaagggaga aaaaacgccg ccgcccaatg tgtggctgcg
     3181 cgccgcaacc aaaggtggtc gcactcgcat cttttctctc aaaagcggcc aaaatcgggg
     3241 agcagtcctg atttttcttg cgcaatttgt gcggggaacc gggcgcctgc ccggccgtta
     3301 atccccgtac gaagagggtc agaggggact tcgtcatatg cgtcgcgttg cgagtatcct
     3361 gccgttcacg atcagtcccg cgacgtggat ggccgcgatc gttgtggcgg cggtgtcgat
     3421 tgcggcctcg ctcctcatcg ccagtcgcat cgcctcgacg gagaagcctt cgttcccgca
     3481 gagtgccttg atcctgcccg agccgggcca gacgccaaac cgcgcggcaa aggccgcgcg
     3541 cgagccctcc aagcagacgc ggcgcacgcc tgatccttcg agggccgctt cgcggccacc
     3601 tcaggatgac ccttcgaggg ccgcttcgca gcacctcagg atgacgcttc gagggccgct
     3661 tcgcagccac ctcaggatga cgtttcgagt ctcgtcacgc ggcgccaagc accaaagcgt
     3721 gagggtggtg cgtccgccga gacgggccgc gcctcctggt acgatttcga cacgaagacg
     3781 gcgagcggcg aagcgatgaa cggcgacgct ttgaccgcgg cacatccttc gcttcccttc
     3841 ggcagcaagg tcaaagttgc caatctcgac aatggccgcg aagtggtggt gcgcatcaac
     3901 gaccgcggtc cgttcagcaa ggggcgcatt atcgacgtgt cgcgagcggc ggccgaaaaa
     3961 ctcggcatga tcgcggcggg cgtggcgcga gtcagcgtgc atccgatcgc cgacgcggtc
     4021 gcgaacgatg agctcgcgag cgcgggcggc aaagcgccgc cgcggtagca ttgagcctca
     4081 tgctgaggag cacgctgaaa gcatgcggct cgaagcaccc tacgcagtga gggacatcgg
     4141 tctcggtgct tcatctcgct gaaacgaaac ggtatggatg cccggacaag cttgcgcccg
     4201 ggccggcgaa gccggacccg ggtgtccggg catgacgacg cgtgcgggct gccccctcaa
     4261 gtcgaagccg tccttcgaga cggccacgaa cgcggcctcc tcagggtaag gtcaattgtc
     4321 gaggaaactg cggagctttc gcgagcggct cgggtgcttc agcgtgcgca aggccttggc
     4381 ctcgatctgg cgactgcgct cgcgcgtcac ggagaattgc tggccgactt cttccagagt
     4441 gtggtcggtg ttcatgccga tgccgaagcg catgcgaagc acgcgctcct cgcgcggcgt
     4501 gagcgaggcg agaacccgcg tcgtggtctc gcgcagattc gactggatgg cggcgtcgat
     4561 cggcaacacc gcgttcttgt cctcgatgaa atcgccgaga taggaatcct cctcgtcgcc
     4621 gatcggggtc tcgagcgaga tcggctcctt ggcgatcttc aacactttgc gcaccttctc
     4681 gagcggcatg gcgagcttct cggagagttc ctccggcgtc ggctcgcggc cgatctcgtg
     4741 cagcatctgg cgtgaggtgc gcacgatctt gttgatggtc tcgatcatgt gcacggggat
     4801 gcggatggtg cgcgcctggt cggcgatcga gcgggtgatc gcctgccgga tccaccacgt
     4861 ggcgtaggtc gagaacttat agccgcggcg atactcgaac ttatcgaccg ccttcatcag
     4921 gccgatattg ccctcctgga tgagatcgag gaattgcagg ccgcgattgg tgtatttctt
     4981 ggcgatcgag ataacgagac gcaagttcgc ctcgaccatc tccttcttgg cctgccgcgc
     5041 ctcgcgctcg cccttctgca cgatatgtac gatcctgcgg aactcggaga tctgcaggcc
     5101 cgtctcggcg gcgagattct gaatctcgga acggagctcc ttcaccaaac cgcgctcgtc
     5161 gcggatgaaa tcgatccagc ccttcttctt gaggcggccg acgcggcgga gccagttggg
     5221 gtcgagctcg ttgccctgat attcgccgat gaaatcctcg cgcgaaacgg cgtagctctc
     5281 ggcgaggcgt agcaggcgcc cctcatagcc gagcagcttc ttgttgatgt cgtagagggt
     5341 ctctacaaga ctctcgatac gattggcgtt gagggagagc gacttgatct cggccacgat
     5401 ctcgtccttg agcagcttgt agcgcttctc ctggcctgac gagaggctat cgttcttgag
     5461 cgcgcgctcg acctgctggt cctgaagctt gcggagcttc ttgtagttgg tggcgacgag
     5521 gtcgaaggtc tccagcacct tcggcttgag ctcggcctcc atggcggcga gcgacagcgc
     5581 gttctcgaga tcgtcctcgt cgtcttcgaa ttcctcgggc tcgggctcgc tctcctcgtc
     5641 ggcgcctgcg cgggcgccgg ccacgcccgg cgcggcctgg ccattgccat tggtctcgcc
     5701 atgtcccggc tcgccgggaa gcggctggga cggcgcgggc ttggcgtcgg ggccctgata
     5761 ggtcgcttca aggtcgatga tgtcgcgcag cagaatcttt ccttcgttca gctcgtcgcg
     5821 ccagatgatg atggcctgga aggtgagcgg gctctcgcac agcccggcga tcatcgcctc
     5881 gcggccggcc tcgatgcgct ttgcgatggc gatctcgccc tcgcgcgaga gaagctcgac
     5941 cgagcccatc tcgcgcaagt acatgcggac gggatcgtcg gtgcgctcgg atggctcctt
     6001 cgtggtctcg gccttggcca ccggctgcgg cgccaccacg gcgcgcgggc tttcgcctcc
     6061 ctcttcgcct tgttggggcg tttcttcggc ctcgtcggtg tcgatgacat tgatgcccat
     6121 gtcggacagc atcgacatgg tgtcctcgat ctgctccgag gagacttcct ccgacggcag
     6181 aacctcgttc agctcgtcgt aagtcacgaa gccgcgcgcc ttggcggtct tgatcagttt
     6241 cttgactgcc gcgtcggaca tgtcgagcac ggggccgtcg gcgggctccg cttgggtttg
     6301 ctcctgatcc tcggcttcag cggctttggt tgccattcat gttactccgc agcgcaagat
     6361 tcgtttggtg gcgagcgcac gctctgcgcg cgccggtcgt tgcagcgctc cgcccaggcg
     6421 caacccccac aagcgattaa cgggtgctcc gttaatcgtc aattaagcat ggacccactc
     6481 cctagcctgg cgcgggactt ttttactcat gccaagcttg ggtcgactgt aaagctccct
     6541 atggaaaaca cgcccaaagc acgaaggcca aggctccgcg tgctgtttac ttgcacaaca
     6601 ctagcaatcg aggcgactcc ggcaagatca gcttgacgcg caggggtccg tttcagcccc
     6661 tgaacccggg cctcgtcaag cccgttcagc cccaaaaacg atggttgtgt ggcggttgca
     6721 cggcttcttc atcacttggc gaggggggca gcgcctgtca aggccagggg cctctctcag
     6781 caaggatttt ctgggaattg acttggctca tcccggccgg gctcgcaaga ccgctcccgg
     6841 cttaagctct agctctgttt ctcgggacgg cgcccgcgtt gggccccgat attcgcgccc
     6901 ttcaggcggg cgatggtttc ctcgaagcgc ttggcggcgt cgcggtcgcc ggcatcgtcg
     6961 gccgtgtgct gattgccgat gctcgcgacc tgctgctgaa gcgctgcgaa gcgatcgaac
     7021 tcggcgtccg tcacatcggc gagacgatcg gcgctctgct cgagctcgcg ggtcaggttg
     7081 ggcagtgccc ggaaccggta gatgaggtcg gcgaattgcg cctcgacctc ttccttcggg
     7141 ctttgccgat cgaggaaggc aagacgcgtg aacgcactct cgcgcatgag cttggcgaag
     7201 accttgccgt gactgctcgc tgcgacccgt tccgcgacgc gttcatccgt caggtgtccg
     7261 tcctcaagga tgatgccgat gaccgtccgc agcaggctcg caagttttcg gtccctgatg
     7321 tcgagtgccg atacttcctc ggcgaaacgg tcgagcagcc acggatggtt gacgagcgcg
     7381 agcacgatga cggtggcgaa gccgtcaggc gagggattgg ggggcggccc ggcccgccca
     7441 gcagaccgcg gcgcattcgc aggcggcttg ccgcggaaac ccttctcgcg ccaaagagcg
     7501 aagagccggt tcttcacctc gcgccggtag tgctccttga cgcgcgcgtt ggcgatctct
     7561 gcgagcaggg tctcgagccg cgcctcgaag gcggcgcggc gttcaggcgt atcgacggcg
     7621 tggagcttca gctcgcgctg ccagagcaca tcgatcagcg gccttgccga ggcgacgcat
     7681 gcggaaagtg cacttgcgcc cttggtccgc accaggtcgt cggggtctga gccctcgggg
     7741 aggaaggcaa aacggaacga gcggccttcg cgcaaattgg tcagcatgcg gtcgatggcg
     7801 cgagcggccg ccgcttcgcc tgccctgtcg ccgtcgaaac agacgacggg ctccgccgcg
     7861 agctgccata gcaattccat ctgccgttcg gtgaaggccg tgccgagcgt ggcgacggca
     7921 tttgcgaatc ccgactgatg cagcgcgatc acgtccatat agccttcgac cacaatcagc
     7981 tctccgatat cgaaggcgac tttgcgcgcg cgcgcgaagt tgaacacggt cgagcccttg
     8041 tcgaacagtc tggtctcggg cgagttcagg tatttcggct cggcttcggg atcgagcgcg
     8101 cgggcgccga aggcgataac gcgcgatttc gcgtcaagaa tgggaatggt gagacggttg
     8161 cggaagcgat cgtaggtcgc gcgcccatcc tcgggcttga tcaggagccc ggcttcgatc
     8221 agctgcgctt cgctgaaccc tttcttcagg agcgcgggct tgagcgcgtc cttcgcgttc
     8281 ggcgcgaagc cgatgcggaa ctccttcaac gtgtcgcgcg acaatcccct tccctcggcg
     8341 taagcgaacg ccacacgccc ttcgcgcgct tgaagcgcct cttcgaagat gcgcgccgcg
     8401 gcttcgagcg catcgaacag gccgagccgt tctttcgccg tgcgttcgaa ctgcgggtct
     8461 ggcttcggca gctcgagacc cgcttcgccc gcgagcctct cgacggcctc gggaaacgag
     8521 agcccctcgg tctccatgac gaaggtgaag atgtcgccgt gcttgccgga cgagaagtcg
     8581 tgatagaagc ccttctggtc gttgaccgtg aaggacggcg tcttctcctt gttgaagggg
     8641 ctcaagccct tccactcgcg gccggcgcgc ttcagaggca cgcgccggga gaccacctgg
     8701 gaaacaggca gccgggcgcg gatctcgtca aggaacgaat cggagaactt catgagttga
     8761 tgaggacgct cgcccttgac tgaaagccat atgctccttg ccgctattcc cactattcac
     8821 tattgcgttt cgtatctacg tttagtatat acatgctgac aaatctaggg ggacggcccc
     8881 ctccaagtcg taggtgcgtg agcgtgtcgc gttcagctct tccaacttgg gagggatcgt
     8941 tccccttaga agcaaatgca gacggcggat tttgatcctt ccgaggttga cgcgtttgag
     9001 tgggatgagg cgaagcgcct ggcgaacatt gagcgtcatg gcatcgactt tcgcgacatt
     9061 ggtcggtttt tcgcgagtcc aactttgata tcgcgctcgg atcgaacgag cgaaatccgc
     9121 ctgggttgca gttggcatcc tcaacgagcg agtgatcagt gtcatcttca cgcaaaggca
     9181 tagcagctgc cgcatcatct cggcgcgccg ggcgcgccgg aaagaaagac tcgcatatcg
     9241 caagctacac ccgggaggag cttgagcggt tccccagcga gagtgattgg gcgcgcgtgg
     9301 acgcgatgac tgaggaggag atcgaggctg cggcccgtgc cgatccggaa tgggcgggtc
     9361 tactcgatat tgactggagt ggagccaagt tcgtcatgcc gcgccggaaa gaggcgatct
     9421 cgatcaggct ggacgaggac gtgcttgcat acttcaagag ccttgggacc ggttatcaaa
     9481 cacgcataaa tgccgtgctc cgacacttcg tggagcagac gagagccagg cggaaataac
     9541 gaggcacgcg cccctcacac gaagtgggat agatcgaaga ctatgttgcg acgggcggca
     9601 gtggcttgca caccgcaagc cgcaacagac ttgctcactt attttgcgcc attgggtggg
     9661 ccggggaaga tcagctcggc gggcgccacg acggcgagcg tcgcagcatc ggctccgcaa
     9721 agcaggctca tcgattggcg ctgcgcgccg tctagcgcga gcgtctggcc cggcttcagg
     9781 ctgacgcgaa cacgcgccga ggcgtagggc gcgcttgcat cgccttcctt ccacgtcacc
     9841 gcgctcagtg cgcatccccc gcgttccgga acgaagaagg cgatgacacg ctgggtgccg
     9901 accgtcacga gaatcggctc gcggggtcca acgggcccga gattgtgcgt gggcggttcg
     9961 gcggcgagaa cgggcgcgcc ggcggcggtg agcgcgagcg caagtaaggc gctcacggcg
    10021 gcgcgaaaac gagaatgtga aaactttgcc attgatggcc tccttttcaa gcaagcaacc
    10081 gaatcaattc gtgctgcatt gcggtaaccc tattgcaatg tagtgacgat tcgatgttgc
    10141 agtgcgaaga caaaaggccg cgtggcgtcc cgccgcaggc aaaatgcgcc ggcgcaatct
    10201 ggatttccga tcatggggct ctcgaagatc gagcggcaac ccgggttgtc attcccgcga
    10261 aggcgggaat ccagtaaccg ccgcgtcgcg agcctgcacc gctcgcgcgt tactggattg
    10321 cccggtcctg cctgcgcgaa gccgaagcgc ctgctgcatc aaaacgccac caaggggcgg
    10381 cttcgcttcg gcgaaggcag gcaagccggg caatgacgaa ggcgtgtgaa cgagcgcgaa
    10441 ggactagccg agtttcgttt ttacgagcgc gctcgcgcgt gcaaagtcca tctgcccggc
    10501 gtagcgggct ttgagcgcgg ccatcacctt gcccatgtct ttcgcgctcg acgcaccggt
    10561 cgcggcgatc gcatcggcga cggctttgtc caactcttcg cccgagagct gctgcggaag
    10621 gaactcgcgg atgatgacga tctcctccgc ctcctgcttg gcgagctcgg cgcggccggc
    10681 ttcagcgtag atcttctccg attcctcgcg ctgcttgatc atcttggcga agagctcgag
    10741 cagctcggcg tcggtcgctt gcgtcttgcc cgcggtgcgg gcggcaatgt cgcgatcctt
    10801 gattgccgcg ctcatcagcc tgagcgtcga gatgcggcgc ttgtcttgcg ccttggtggc
    10861 atgcttcaag gctgcggtga tggtctcgcg catcgatctt cgggagcgtg tgggaaggaa
    10921 gtttccaaac taatcgaggc ccttcgccca ggcaagagaa tttctcctct taacttattg
    10981 tttcacaaca aaatattttg ccctgtggat tgaccgctct ggtctcctgg cctaagagtc
    11041 cgcctgcccg gcgcaagata cgtgggcctc aagcccttgt cgtagcattc cttgcacccg
    11101 acttagcaaa ccgggcgcct cccgccgaac ggcgcctata agagacgatg accgacacgc
    11161 gtgaaactct ggccgcagag gcccagccgc cgcgcgcgcg cgccgaagcc gggtggaccg
    11221 acgagacgcc gacgggcctg ctcgttctcg ccgacggcac cctcatcgaa ggaaagggcg
    11281 cgggcgctgc cgggatcgcc gtcggcgaag tctgcttcaa caccgccatg accggctatc
    11341 aggagatcct gaccgatccg tcctatgccg ggcagatcat caccttcacc ttccctcata
    11401 tcggcaatgt cggaaccaac agcgaggaca tggaggcggg cacactcgcc gctggagggg
    11461 tgcggggctg cgtgctccgc gcgccgatca cgcctgcctc gaattggcgt gccgcccaag
    11521 gtctcgacgc ctggctcaag gcgcgaggca tcatcgccgt gaccggagtc gatacgcgcg
    11581 cgctcaccgc gctgatacgc gaaaagggca tgcccaatgc cgtgatcgcg catgcgccgg
    11641 acggcaagtt cgaccgggag gcgctgaagc gcgaggcggc cgctttccct ggcatggtcg
    11701 ggctcgacct cgccaaggac gtgacctcgg gccaaagcta cggctgggac gagacggcgt
    11761 ggcagtgggg caagggctat ggccggcagg agagcccgcg ctttcacgtg gtcgccatcg
    11821 attacgggct caagcgcaac atcttgcgcg agcttgcgac cgtcggctgc cggctcacgg
    11881 tcgtgcctgc gacaacgacg gccgaggaaa ttttggagcg gaagcccgac ggggtgttcc
    11941 tgtcgaacgg tccaggcgac ccggcggcga ccggcgtcta tgccgtgccc gagatcaaga
    12001 aactcgtggc ctcggggctc cccgtcttcg gcatctgtct cggtcatcaa atgctgggac
    12061 tcgcgctcgg cggcaccacg gcgaagatgc atcagggcca tcacggcgcg aaccacccgg
    12121 tgaaagactt caccaccgag aaggtcgaga tcacctcgat gaaccacggc ttcgcggtcg
    12181 accgcgatag cctgcccaag agtgtcgagg agacgcatgt ctctttgttc gacggctcca
    12241 attgcgggct gaaggtcaag gacaggccgg tgttttcggt gcagtaccat cccgaggcat
    12301 cgcccggtcc ccaagacagc cactatctct tcacccgctt cgtcgcgatg atggaaaagc
    12361 gaaagggtgc ggacaaaggc gagagagagc agtgagggag ctagtagcaa ccgacaagtc
    12421 ccctctcccc tccggggaaa gggctagggt gagggggatt tcattctgct gtctcgcttt
    12481 cgagcacgga cagaatgatg gtcagcacgc cttcttgatt ttgcaacact tccgaattcc
    12541 aaaagcgcaa gacacgatag ccttgccgag caagggcagc gtcgcgccgc cgatcgcgct
    12601 cgcttgcatt gtgctgacct ccatcaaact cgacgaccac gcccttttcc ttgcaaagaa
    12661 aatcgacaat ccaaggaccg accggcactt gacggcgaaa cttccaccca gcaaggcgtc
    12721 gatctcgaag ctggtaccag agtttgcgtt cggcgtcggt caggtccgcc cgcaagctgc
    12781 gcgctcgatg cgtgccaatt tgtttcatac cccctcaccc caaccctctc ccctccgggg
    12841 agagggagag cgcactagtc taccccatgc ctaagcgcac agacataaag tcgatcctga
    12901 tcgtgggcgc aggaccgatc gtcatcggcc aggcctgcga gttcgactat tccggcaccc
    12961 aggcctgcaa ggccttgaag caagagggtt accggatcgt gctggtgaac tcgaaccctg
    13021 ccaccatcat gaccgacccc gaactcgccg acgccactta catcgagccg atcacgccgg
    13081 agttcctcac gaagatcatc gagaaggagc ggcccgacgc gctgttgccc accatggggg
    13141 gacagaccgc gcttaacgcc gcgctcagcc tcgatcgtgg cggcgtgctc aagcggtacg
    13201 gcgtggagat gatcggcgcg cgcgccgagg tcatcgacaa ggccgaggat cgcgagctgt
    13261 tccgcgagtc gatgcacagg atagggctcg aaacgccgcg gtcgcggctc gccgatgccg
    13321 ccacgctgaa gcgcgccgac cggcagaaaa ataaggacgc gatcgcgcgc atcgagacct
    13381 cgcacctcga tccggaggag cggacacggg cgctttccgg gttcgaggcc gagtgggcgc
    13441 gtggcgagag cgagcggcgc cggcgttatg tcgagaaggg cttgatcgaa gcgctcgatg
    13501 cgctccccga tatcggcctt cccgccatca ttcgcccctc cttcacgctc ggcggcaccg
    13561 gcggcggcat cgcctataac cgcgaagaat tcctcgagat cgtcgagcgc gggctcgatg
    13621 cttcgcccac ctgcgaagtg ctgatcgagg aatcggtgct cggctggaag gagtttgaga
    13681 tggaggtcgt ccgcgacaag gacgacaact gcatcatcgt ctgctcgatc gaaaatatcg
    13741 atccgatggg cgtgcatacc ggcgattcca tcacggtggc gccggcgctc acgctcagcg
    13801 acaaggaata ccaagtcatg cgcaacgcct cgatcgcggt gttgcgcgag atcggggtgg
    13861 agaccggcgg ctccaacgtg cagttcgcgg tgaaccccgc cgatggaagg ctggtcatca
    13921 tcgagatgaa cccgcgtgtg tcgcggtcct ccgcgctcgc atcgaaggcg accggctttc
    13981 ccatcgccaa ggtcgccgcc aagctcgccg tcggctacac tctcgacgag ctcgccaacg
    14041 acatcaccgg cggagcgacg ccggcctcgt tcgagcccac catcgattac gtggtgacca
    14101 agatcccgcg cttcgccttc gagaagttcc cgggggcgca gcctgtgctc acgacttcga
    14161 tgaagtcggt gggcgaggcc atggccatcg gccgcacctt ccccgagtcg ctgcaaaagg
    14221 cggtgcgctc catggaaacg gggctcacgg ggctcgacga catcaccttc gaaggcctcg
    14281 gccagggcga cgacaagaat gtgatccgcg ccgcgctcgg tacgccctcc cccgaccgcc
    14341 tgctcaaggt ggcgcaggcc ttaaggctcg gcttcgagga ggcgcagatc ttcgaggcgt
    14401 gcaagatcga tccgtggttc atccgccaga tcaaggacat cgtcgatatg gaggcgcgcg
    14461 tgcgggagca cggcctcccc gccaatcccg gtcaattccg cgcactgaag gcgatgggct
    14521 tttccgacga gcggctcggc aagctcgcag gcgtggaggc gagtgaagta tcggccatgc
    14581 gccggacgct cgatgtgcat ccggtctata agcgcatcga cacctgcgcg gccgagttcg
    14641 cctctccgac cgcctatatg tattcgacct acgaggctcc cttcgcggga cgtgcggaat
    14701 gcgaggcgcg gccgacgcaa gccgagaaga tcatcattct cggcggcgga ccgaaccgca
    14761 tcggccaggg catcgagttc gattattgct gctgccatgc ggcctttgcc ctgtcggatg
    14821 cgggctacga gaccatcatg gtcaattgca atccggaaac ggtctcgacg gactacgaca
    14881 cctcggatcg gctctatttc gagccgctga ccgaggagga cgtgctggag atcgtccgca
    14941 ccgagcggca gaacgggacg gtgttgggcg tcatcgtgca gttcggcggc cagacgccgc
    15001 tcaagctcgc ccaggcgctg gagcgcgcgc aggtgccgat cctcggcact tcgcccgatg
    15061 ccatcgatct cgccgaggac cgcgaccgct tcaaggccct gatcgagcgg cttgaattgc
    15121 gccagccccg gagcggcatc gcccgctcgg caaaagaggc caaggcgatc gcggcggaga
    15181 tcgggtttcc gctcgtcatc aggccttcat acgtgctcgg cggcagggcc atggagatcg
    15241 tccacgacga agcgcagctc gatcgctaca ttgccgaggc ggtggtggtg tcgggcaaga
    15301 gccctgtgct tctcgactct tatctgcgcg acgccatcga ggtcgatgtc gacgccatcg
    15361 gcgacgacaa ggacgtattc atctgcgggg tgatggagca tatcgaggaa gcgggcgtgc
    15421 attcgggcga ctcggcctgc tcgctgccgc ctcattcgct gaaaccggag atcgtcgccg
    15481 atctggagcg ccagaccatc gagctcgcgc gcgcgctgaa tgtggtcggg ctgatgaacg
    15541 tgcagttcgc catccagcac gacgacatct acattctcga ggtcaacccg cgcgcctcgc
    15601 gcacggtgcc cttcgtcgcc aaggtgatcg gcttacccgt cgccgggatc gccgctgagg
    15661 tgatggccgg gaagaagctt gctgattttg gactgaagcg gccgaagctc aggcatgtcg
    15721 cggtcaagga ggcagtgttc ccgttcgcgc gcttcccggg cgtcgacccc gtgctcggtc
    15781 ccgagatgcg ctcgaccggc gaagtgatgg gcatcgaccg caactacgct gtcgctttcg
    15841 ccaagagcca gctgggcggc ggctcgcggc taccgctgtc cggcaccgtc ttcgtctccg
    15901 tcaaggatgc cgacaaggac aaggtgctcg cgcctgtccg cgcgcttgcc gagatgggct
    15961 ttcgcatcat cgccacgcgg ggtaccaagc gtcatttcga cgcccacggc atcgcgtgcg
    16021 agagggtgaa caaggtgctg gaggggcgcc cgcacgtggt cgacgccatt accaatggcg
    16081 aggtcgatat cgttttcaac acgacggaag gagccaaggc acttgccgat tccttctcca
    16141 tccgccggtc cgcgctgctc taccacattc cctactacac gacggtggcg ggcgccctgg
    16201 ccgtgaccga ggccatcaag gcgctcaggg ccgggagcct gaacgtggct cccttgcaga
    16261 gttatgtcgg cacccgcccc tacaatctcg ccgagacggc ctaaaaaacg ggtcaaaatc
    16321 aacggattct tgacttagac tgggcaattc ctggacagta tccggtccgg cgagcaaagg
    16381 agctttgact gcgatggaga aggtgccgat gaccgcggag ggctatgccg cgcttgaggc
    16441 tgagatcaaa tatctcaaga ccgtcgaacg cccgcgcatc atcaagcaga tcgccgacgc
    16501 ccgcacccat ggcgatcttt ccgagaacgc cgagtaccac gcggccaagg aacagcaggg
    16561 cattaccgag gcgcgggtcg ccgatctcga ggacaagctg tcccgtgccg atatcatcga
    16621 cgtctccaag ctcaagggag accaggtgat gttcggggcg accgtcacgc tgctcgacga
    16681 ggacaccgac ggcaaggtca agtaccggat cgtgggcgaa ctggaggccg aagtgcggag
    16741 cggcaagatc tcgatcacct ctcccatcgc gcgcgcgctt ctcggcaagc gcaaaaagga
    16801 catcgtcgag gtgtccacgc ccggcggcgg caagtcctac caggtgctca aggtcgaata
    16861 caaataggct tggccggtct tgcccggacg ttggcgactt tcccgtctta ggatttaacc
    16921 cctcaaccga tcgtaacgga atttctcatg aggctcggca tgtcttgctt aacggtccgc
    16981 gcgattgcgc tcgcctgcgt catcttcttc gccgccggca tggcgtctgc cgaaacggtt
    17041 ggctcaatta cggtcgagaa tgcgtggtcg cgcaccgtgc cgagcggctc cagtgtcgcc
    17101 gccggctatc tctcgatccg caacgacggc gacgagccgg accgcctgct ttccgcaagc
    17161 gcatccttcg ccagcgcgac tgagatccac cagacgacga tgtcggatgg agtgatgcag
    17221 atgcgcccgg tcgccgacgg ggtgatcatt ccagccaaga gcacggtcat gctggagccc
    17281 ggttcctatc acctcatgtt caggcaactg accaggccga tcaaggaagg cgacacgctc
    17341 ccggcgacgc tggccttcga gcgggccgga aacgcggaca ttacgatcca agtcctcggc
    17401 ataggggcgc aggggccgaa ggcggagccg cggcaataag gctgaaaggc gagcttgcgg
    17461 cgcctcttcc cggggcatta cgctcaaagg tagaagggat agcgttcggt atgacaagga
    17521 acaaggatcg tcgcaggtca catcacaacg aggagtacaa atcatgattt caaggccgct
    17581 cgcgttgact ttgcttgctt gcatcgcgtc cgccacgctt gcgctcgccc atgagggtgc
    17641 aaccggcgtg gtcaaggagc gcatggatct gatggaaagt cagaaggaag cgatgaaggt
    17701 gatcggcgac atggcgaggg gcaagaagaa gttcgatgcg gcggcggcga ccaaggcagc
    17761 caacgatatc agctcgaccg cgaagaaaat ccccgagctc tttcccgagg ggagcggtgg
    17821 cggcaagagc gacgcgctcc ccgcggtgtg ggagaagtgg gaccggttca ccgacaatgc
    17881 tgaggacctc gccaccaacg ccgatgcgct cgtcaaggcg ctggccgaca gcgccagtgc
    17941 ggcgtggaag cccgcgttcc agaaggtcgg cgacacgtgc aagtcctgcc acgaggactt
    18001 ccgtgccgag aagaaggaaa agcaccatta agcgcccctg ggggatcggc gcgggtttaa
    18061 atttgcacgg cctgctatgg cgctcggctg ccttgttgcg atgatagagt tcggccggtc
    18121 cggcatggcg acatgatcga aaagctcgaa ttcatcatcg cgctcgcacg cgaacgaagc
    18181 caaccagctt gccccgatcg ttggcgccga gactcccccg gagcggttgc ccaaacgcgc
    18241 tgcaaggccg cccgcccgct gacatgcgac ttcttccgca cgggcggccc cttacggggt
    18301 cacggcatga cggcaacacc ccagggcagg cgtccgaccg ggaccgactt ctccgccttc
    18361 agcgattcga cgtcgatgat cgtgatgtcg ttggtaagcc cattggcgac gtaatacttc
    18421 ttgccgtcgg ggctaagctc gccgtgccaa ggacgctggc cgaccaggat gtactccttc
    18481 acctccttcg ttgcggtgtc gatcacggca acgcgattgg acggtccgag cggaacgaac
    18541 atcagcttgc cgtcgaggct gaagtccatg cccacgggct gaagctgctc ggggcgcacg
    18601 ccctcgacct caaagccgat cttcttgacg actttccagc ttttgggatc gtcccagttg
    18661 ctggtgtcga caatcgagat cgtgccgcca atctcggagg agacccacaa ctccttcttg
    18721 tccttggtga atttcgcctc cctcggcctg ctgtccacga ggatattgcc caccagtgtc
    18781 cgcgtcttgt tgtcgatgat atgcgccatg ctggtgctct cggacgtggc ggcggtgtag
    18841 cggtcgtcgg gactcacagt catgccttca ggctcgacgc cgaccggcac ttcgccgagg
    18901 acctcgccgc tcttgatgtc catgatggtc acgagcgaat cgtcttcgtt ggcgatgtag
    18961 atgatctcgc ctttcgaatc gacggccatg agctcggggt cgggacccga ctcgagctgg
    19021 cgggtcactt cgagagtttt tgtgtcgatc acgtccatga tgtcgccgtc gcccgcggca
    19081 acgaacagct ccttgaaatc ggggctcaga atgatgccgc gcggacgccg cgcgaccttg
    19141 accgtcttga tggtctccag cgtttcgccg ttgagcacag tgatcgtgtt gtccttctcg
    19201 ttggcgacga agatggtcgc ggcgaacgaa ggcgcggcag agagaatgag cgcggcgctc
    19261 aagaccgcgg cgaggctggt gcgagtcatg gttctaccct tggtcaatgg ttatttgaac
    19321 ttgcacttgg tctccggcgc atcgaagcca agcgtgtcgg tgaggttggt gggatgcagg
    19381 aaaccctcct gcggcgaggt ggacaccggc acgcgggtgc cgcctccgag gatgatcggc
    19441 tggcgcattt gatggtccca ggtgcggaag ctcatggcct gtcctttgaa gccctcgagc
    19501 ttgaaggcgt ctgacagcat gaaagccttt acatcctgag gcgcggtctt gccggatttc
    19561 atcgccgaat cggcgagagc gcgaaatccg agccaggcgg tgtaatcgcg ctcaacgggc
    19621 gtgcgtttgg caatgcgcgg aatggcgttc tggaaatgca tgccgcccga ttccgtgtag
    19681 gacttgtccc aggccgtcgc ttgcagccct tgggtgccga ccacgggacg cggcagatag
    19741 gcccgataca tgaggtagtc gccgaaatcg tcgtcgctat tgagcaccca gaccacatcg
    19801 tgctcggacg cctcctgggt ctccatgggg atttgcgcct ggatctgctg gtggcccgag
    19861 tccagattgc gggcgcccgg cggcagagca tagaccttgt cggtgacaac cttgccgccg
    19921 aagcgggcgg cggagcgctt gacctgggcg agatagtcct ggtcttccgg cgtgtcgcgc
    19981 ctgatgacga accatcgcgg ccacttcttc cagatcagat attgcgccag cgcgtctgcc
    20041 cgcatcgcat agtcgggaat cacatgaaag acgttgacgc ggcactgctc ctggcggagc
    20101 gcggtcgcgc tcgagcggat attcatgatg accgcgtctt tggcatctgg caggtcggca
    20161 acggcgagga gatcggccgg ctcgagatcg gcgatgatga agcgctcgcc ggcggcgaga
    20221 agctccttgg ccttggcgac gacgtctccg tcctcgggca cgatcgcctc gatgagctcg
    20281 aaggagtgac cgacgaaagt tccggcctgg tttgcctcct tgagcatcaa gagggcgcct
    20341 tgaatgccct tatcgataat gggtttctcg gcataggaca ggggaagagg ctcgggatat
    20401 tccttgccga gatagacgat ccgcatgttc tttgcggcgc tgcttccggg cagcgcgggc
    20461 gtggcggcag caggcgccgc cggtgcttgc gttccagccg gtgcgctcgg ggcttcggcc
    20521 gcgggagcag gaaccgtcgc cggttccggg gggtccggtg ccgcggcgtg cgccgaagca
    20581 actacggctg gaacgaaaag cgcgagagca acgagacttg cgagaatttt gcggcacacg
    20641 cggaagcggt caaatggact tctcaacatc ggcgagagcg gtgctcctac ggggctgatg
    20701 atgaaccggg atgggacgag cgatctcgct cgaagattgg ggaccaggac acgatcttcc
    20761 aaaactgcca gaacttggca aggcgtggtg cacaataggt aagcgcgtgc cgaacttaat
    20821 cctcgccccg ctcgattcta tatggggaag cggcaaaagc gcttccaggt cgcggcaatc
    20881 ttccatgcca aaagtgtcat tgcgcgatcg gcactggaaa agccgccaag ccccggcagg
    20941 aactcggcgg cttaccaaaa attcatgtgt cgctcgaacg tctattgaag cggcggcttt
    21001 ttctcggagc cgggaggagc caccgaacgc atccacgcga tgaccttcca caaatcgtcg
    21061 gaggttttga tgatgccacc gaagggcggc atggggccca ccacggtctc gctcttgacg
    21121 cgcttgtaac ctgccttggt caagtcgaca ctcccgagcg cgatgagcct gaacaacacg
    21181 tcgtcgtccg ggccgtagac ccatgtatcg ttggtcagcg gaatgcacat gccgccgccg
    21241 ccgccgccgc cgtgacagcc gttgcagcta aaggagagat atttcttgtg accctcctca
    21301 gccatctcgg cgatcttgtc gttgtaaggg ttgtgcagcc ctcccttggg cgtggccttg
    21361 atcaggtcga gcgggggcgt cgtgtcgccc gccttgcagc actccccgcc accggcgctc
    21421 gctgctgcgc cttcagccgg cgcggctggc tgcgctggag cggcctcccc ggtcggctgt
    21481 gccggcgcgg ctggctgcgc tggagcggcc tgcccggtcg gctgcgctgg ggcggctggc
    21541 tgcgctggag cggcctgctc ggccggtgcg tccgccgcaa tggccttcca cgcgaaggtc
    21601 tccatgccgc caataatcac gagtgtgaaa agtccggtga tgatcttgtg cacttttggt
    21661 ttccttgccc tagctggagt cgttggttgt tgatgctttc ggtcgattgc agatgccctc
    21721 agtgatagcg ctgtccgtga caaagcgata gcagccgttg aacgatgcgg caatggttct
    21781 tccatcagat taacggagat acttcaattt gttacgtcga aatccatgcc ctcgaacgta
    21841 tagcgcggca cttgcgtcca gatgccgtcc tcctggaccg ggaccttgtg catcttgaca
    21901 tccgcgaccc tgagttttcc cgacttggtc tccagccaga agtcgatgtc gtaatattcg
    21961 tccttgctgc cgggcttgcg gaaatcggtg caggcaaaat accgaccgtc ctccttgaga
    22021 cgcctgatcg gctggtgcat ctcgatgaat tcgagcggaa ctgtctcccc gctcttgtcg
    22081 tccttgacga ggaggtttcc gttctcatct ttgttggcgg cgatgtagtt gtggatagcg
    22141 ctcatgacgt gccagccgcg ggtcacctcc atgtcgcccg gatgctcctg aacgggcaac
    22201 caccaccagg ccaccggcaa gcgcgtgacc atgatccacc cgtcgccttc cggcttaggc
    22261 cccttctgca ctctgatgtc catcagcttc agcttgtcgc cttcgggctt gaaccagaaa
    22321 tcgatggcgt attgcttttt cggcacgtcc ttgtcgtgga agatcgtgtt ggcgaaccag
    22381 ccgtagccct ccatgccgcg cacgattttg acctgttcga agacaaggtt gaggtcggca
    22441 tcgagcttgg gatcatggaa gacgaacaca ccgtcctttg agcgttcggc gatgacgtcg
    22501 gcaacggctt gtttgaccgt gctttcgtca acttcagccg ccgaagccgg cccaagcacg
    22561 aggaaaccca atgccgaggc gagagcaaga gcccgcgcga ctagaagcca acgggagagg
    22621 ccttgcatgg tcattggctc gtggtcgttt gggcggcgtt gtccgccggc gaaggtgcgg
    22681 ctgccgagcg gccggccccc agcaccaaaa tggcctgggc ggcctcgaga tttccgttct
    22741 tctcggcgac atcggttgcc gtcaggcctt gcgcgttctt cagcgtcgga tcggcctccg
    22801 catccatgag aagaccgatc atcggtgcgc tgttatgcgt cgccgcgatc atcagcgccg
    22861 tcatgccgtt cttggcctga gcgttgacct cggcgccgcg ttcgataagg gctttggcga
    22921 tatcggtcgg atgggcgcta ccgggtagga atcgcgaacc ctcggccggg gatgtctgcg
    22981 aggcgatgag catcagcggg gtgagaccgt cgccgccaga ggcggcactg acatcggcgc
    23041 cgccctcgat cagcgccttg gccacttcgt acctggcctc ggcgatcgca agcgccagcg
    23101 gatgaaatcc gccagggccc ggcttgtcga catcagctcc gttctccagc aataccttga
    23161 tcgagggaac gtggtcgcgc atcgcggcgg ccacaagcgg cgtggtcccg ccctcatcgg
    23221 cgagattggg atccgccccc agctcgatga gcgtcttgat cataccgtcc ttgcgctggc
    23281 gcgccgcgtt gttgagcggc agccagccct gcgggtcggg cttattcaac tcggcgccct
    23341 tctccacgag gaacttcacc cgttcgatat cgttggcgtt gatcgcgttg ctgagctctt
    23401 gattgaggtc ggcgccctcg gcgatccagg tctcaagctt atccttggtc acgacctggt
    23461 cgggcgacgc aagatcggga cgcgcctcga atttctgatc ggcgaaggcg atgtagctgc
    23521 cgtgggcggg aagatcgccg gcgacgatgc acttgctgca ctgtacgaga ggcacgccat
    23581 agtccctcag gatcttttcg acctcttcgg catgatcctc gagcgcgaat tcgagcatgt
    23641 atttcaaaaa ggcgtcggtc ttgcgcacac cgatggccat ctcgaattcc agcggcactt
    23701 tatcctcctg gagattgacc ggcaggatgg tgatcggctc gcccttcatc gtcttgaccc
    23761 agccggcgaa gggcccgaac acggcggcga cgtcgagatc gccatccacc accttttgca
    23821 ccgggtacca aggctgattc tcgattacga gatcgccatc gtgggtctgg gtctggagtt
    23881 tcacattgtc gacgatgccg cgtctggcaa gtgccgcgcg aatccccgag gtctggaaca
    23941 cgccgatctt gacatccttg agcttggggt catcgagccc ggtcagctcg agtcccttgt
    24001 cgttgcgata gacgaggaca tacggcgtct tatagacggg aaaagtggtc agcaggcgcc
    24061 cgtaattggc cggcatgtcg aacatcacgt cgcacatgtc ctggtcgaaa gtctgccgcg
    24121 tgagtccgcg ctcgatgaag ggtcgccagg agtaaacgac gcgcgcgccc atcgccttgc
    24181 ccagaagggt ggcaagcttg ttctgaaatc cctcctcctt gatgttggat aggggcatgt
    24241 tgcccggatc ggcgcagacc atgagcgact cgagcttctt gcccttataa gccgccttgg
    24301 ccgcagccct gatcgcgatc tgctcgctcg gcgtcaggtc gtcgaattcc ttgctgaggt
    24361 cgcgcttaac ttgtttcttg gcggcctccg caggcaccga gacgagcacg agtgccaggg
    24421 ctgacacgat cgcggtgaga attcgcaaag acatggggga cactactgct gttcagttga
    24481 tgtcggggac gcaagcgaac aaggccggct cgcgttctac ccggggtatt aaagcagtga
    24541 cgcccggtgc cgcaagggca ccgggcgtcg ttaatctagg caggccgtcc gcttattcgt
    24601 agatgcggaa gacgttcagc tcgccaccct gggggatctt gtccaggttg gcagccttgg
    24661 tcatggccgt ggcgccgatc gctccgaact tgtcgttcag gtcaagacca gcggtgaccg
    24721 ggagaccgat ccagccgccg atgccggaat acacgccgac atactgcttg ccgccgacct
    24781 tgtaggtgat cgggttgccg atgatgcccg agccaagctt gcggctccac aggatcttac
    24841 cggaactccg gtcgaccgcg cggaagtcac cgccgagcga gccatagaac gccaggccgc
    24901 cgtcggtcac catggtgccg ccccagttcg gatacggatc cgggatctcc cacagcgtct
    24961 tgccggtcaa gacatcgtac ttcttgatct tgccggtcgt gccctgcttc tcaggataca
    25021 tgtacacgtt cgcgaacacg tagaccgtgc cctggttcgt gtgcgtgcgc tcctgaggct
    25081 cgagctccat gcaccagttg ttggtgggca tgtagaagat gttcggctcc ttgggatcga
    25141 ccgaacccgg ctgctggtcc ttgccaccca tggctgaggg gcaagaggac acattgcgac
    25201 cgacttcgag cggagacttg tcccgcacct tgatcggacg gccggtcttc atgtcgacct
    25261 tctcggccca gtccgtcgtc acgtatttcg tggcgcggag cagggttccg tctgcgcggt
    25321 cgagcacata ggcgaagccg ttacgatcga agtggctgag gaccttacgc ttcttgccat
    25381 cgacatccat gtcggtgagc atgttctcgt tgacgccgtc atagtcccac tgatcgaagg
    25441 gggtcatctg gtaggcgaaa actgcttcgc cgttgtcaac cttgcggccg aagatcgtca
    25501 tcgaccactt gttgtcgtaa tcaccagtgt tgcactcttc gtgggtcttg tcggggcaac
    25561 gataagacgg gctccacagg ccggggttac cggtcgagta gtagaccatc ttcagctctg
    25621 gatcgtagct gaaccatccc caagcggcac cgccgccgat cttataatct tcaccgggga
    25681 aggtcttgat gccgagatcc tggccagcag tcccgaagtg cgggttggct ttgttggtgt
    25741 cggccgtcag gcacacgtcc ttgtcggaac ccgtgctgtg gcaaacccaa gcctccgagc
    25801 cgtccttcag gttgaaggca gcgacacggc cgcgagccgc aaactcgtca ccgccgaagc
    25861 ccatgatcac cttgtcggcg gcgatcaacg gcgcgggggt gatggtctcg cccttctcgg
    25921 gataggcgta tttgacaacc cagacttcct tgccggtctg gccgtcgagg gcaatcacga
    25981 aaccgtcgag agtgccatag acgaacttac cgtcggcata ggaaccaccg cggttgaccg
    26041 tatcgcaaca agcgcgcggt acagcggact cgtcacggtc ggtcttcttc acgtagctcc
    26101 aaaccgcttt cggattgtca ggatcggtca gatcgagagc ctgaacaacg ttgcagtttg
    26161 acatggccgg gcagccggaa accatgaaca gcatggtctt gccgccaacg tccttgatca
    26221 cgaggggctg accctcatgg ccgcggagcg cattcgtgcc ctgcaaccaa gccatctgca
    26281 acttgccgat gttcttggtg ttgatatcgg ccaaggtgct atgacgcgtc agagagaaat
    26341 cccggccggg ggctggccac tggtctggat ctttcattcg cgccagctgc tccgcatcgg
    26401 agtcagctac tgcctggccg gacaagccca tcatcaccgc gatgacggtc ccggccagga
    26461 caccaaactt cattttcatg tctttctcct ctccctagct actcagttca ccgaaagaaa
    26521 gctgagttct tctgcttgcc gaagcgtagt tgccccctca aacggagaga gcaccgagcc
    26581 cttgcaagtt tttcaagctg tcactttgga tatgggaggc tgcccgaaag cgccgaagca
    26641 aaaaaataag cctcgcgccc gctttaggcg gcgggtcctc cgacgaacac atcacgcggt
    26701 gacacctcac gaggattcac agcctagcgc aaagaaagac gcggcgtcat cgaattgccg
    26761 atggaaacgg gcatcccact cattgccaag ctgcccgcac ataattgcca aatgtaatgg
    26821 cattgcaagc aaaaagtgct tcgcgcagtc aaatctgcaa aagttctttt ccctcgctgc
    26881 gctgcatcac gatgcggacg ctgcagtttc tcgtgtcaag cagaaaactt gcataattta
    26941 ggcacagcgc ccggatgtat taacctcgtg atcaaaatcc gcccgagcgt ttgagggggc
    27001 agcagcctaa aggcgtggcg cgggctgatc gcctttccgt cccttctcac ggctggctcg
    27061 gcacagttgc acgagacagt tacggcctgt cccgccgagc ctttgaccag ccggtgcgcc
    27121 aagctaggtc gcgcgcttgc cgaaggcaat aatgccggcg acggggacga caaaaccgat
    27181 cgtgaccgcg gcgacgacga gcgcagcgag gttcgcgtag gagccgcgat ccaccgcaaa
    27241 cagcagattg agatacttgg tctgaagctg tcccgccacc agcgcgatat tcatcagcga
    27301 tgccatcagc gcgaaccagg tggcgcgctt gccggcaggg gcattgatgg cgacgatggt
    27361 gagcgccggg atcatgctca gctcggcgaa gggagcggat gcggtcgtat cgacgagggc
    27421 gatggtgcgg gcgccgaaac cgaacgtcgc ttccgtccat tgatcgagcc tgagcgcgag
    27481 cccgatggtc ggcaaggaca gcaccgtgcc gattatggtc agccacagca gcacctgggc
    27541 aaccggcttg cgggtgatga catcggagaa gagccaagcg ccggcgaggc tgagcgcggc
    27601 gccgatctgc gcgagcgtgc cgtagaaggc ctcgtcgaag ccgagcacgt cgatggtaaa
    27661 ccagcgatag ccctccccta cccccgggct tgcccggtag agaaagatca cgagcgcggc
    27721 atagaagatg cgctggcgat ggacttcgtc gaggtcgccg gctatgcggg caagcatcag
    27781 tgagatgacg gcgagcgaga tggcgaagac gagctcctgc ccgaggggca agccgctgag
    27841 gccaatcgcg acaaccgcgg caccgaacag gaggcctccg ccgagaatgc gccagtcgat
    27901 cggtttcggc gcggtgccgt cgagatggac cagcatggcg ccggtcagcg agatggcggg
    27961 aacggcgagc cccaggagaa agaccgtctc gtagctgtag acctgcgcaa gccagccaga
    28021 caggccgccc accgacagga tccccgacca tagcgcgagc cgccccagga cctgcaccag
    28081 gcccaactcg cggtcgactt ccgctttcgg acgcggcgtg ccatccggcc tcacccgttc
    28141 caccacttcc gtcgtcatgg cgtcggcaat cacgtcctgg agcacgatgc cgatcacgat
    28201 catcagctgc gccgtcacat agagcgcgtt cttgctggca agggtgatcc agcctccggc
    28261 ggcgcccgcg agaatgatca tccccgaggc gaccaggccc gcgccgatga acacataaac
    28321 acggcgctga gagcccagaa ttggaaccga gtcggcaagc tgcccgaaca ccattttgac
    28381 cgtccacggc aaggtcagcc agacggcgag cgcggcgagc tccgccggcg tgaaggtcag
    28441 ggcctccttg acccagaagg tttgcgcgat ggcaatcagc ccaagcgcgc cataggcgta
    28501 atagatcatc agcagcggca gatagcgcag ccgcatggcg cgtatgggcg tgagcagcga
    28561 ccccttcagc ttgtcgagga gcgtgcgccg cgcttcggtg ttgcgggccg gtgaagcaac
    28621 cgtcattgcc gaggagcctt ttccgcgctc ggaggaccaa gccgtttatg atgccggtgt
    28681 atcgccggca agagcgggcc gcgtgcgccg ggacctcggg gcatctaccg tcgcggcgcg
    28741 ttctctccgc aaggaccagc ccttcaccac cgcgtagatc gcggggatga cgatcagggt
    28801 cagcagtgtc gacgacacca tgccgccgat catgggaacg gcgatgcgtt gcatgacctc
    28861 ggcgccggta ccatggctcc acatgatggg caagagtccc gccatgatcg cggccacggt
    28921 catgatcttc gggcgcacgc gctcaaccgc tcccgacatg atcgcggcat ggagatcttt
    28981 gccggtgaag gcacggcctt cgcgttcgcg ctgtttccgc agatccttca acgcgtgatc
    29041 gagatagatc agcatcacca cgccggtctc ggcggcaacg cctgcaagcg caatgaagcc
    29101 gacggccacc gcgacggaga ggttgaagcc gagccaccac atcaaccaga agccgccgac
    29161 gagcgcgaac ggcaccgaaa gcatgacgat gagcgtctcg gtgatgcgac ggaagttgag
    29221 gtagagcaga aggaagatga cgagaagggt aaccggcacg acgatttgca gccttgcctt
    29281 ggcccgctcg aaatattcga actggccgct ccaggtcacg taatagccgg gcgggaaatc
    29341 gacaccttcg gccaccgcct ttttcgcctc ggcgacatag ccgccgagat cgcggtcgcg
    29401 aagatcgaca aagatataga gagcgagctg cgcgtcctcg gtgcggatgc ttgagggacc
    29461 ctgcgcgagg ctcaccttgg cgacctcgcc gagcggcacc gtgccgccgc caggcagcgg
    29521 gatcagcact tcgctcgcga tgtcttgcgg gcggctgcgg tagtcgcgcg gataacggat
    29581 gctgacggtg tagcgctcgc ggccttccac cgtggtcgtc accggctcgg ccccgagcgc
    29641 catcgcgatc acgtcctgga catcgccgac catgagcccg tagcgggcga gctgggcccg
    29701 gtcgggatcc acgttgagat aatagccgct gatggcgcgc tcggcgtaag cggaggccgt
    29761 gcctggcacg tcgcggagca ccgcctcgat cttgcgcgcg agcttctcca tctcgccaag
    29821 gtccttgccg aagaccttga ccccgaccgg cgtgcggatg ccggtcgaca gcatgtcgat
    29881 acgggccttg atcggcatgg tccaggcgtt cgacacgccg ggaaattgca gcgaccggtc
    29941 catctcggcg atgagcttgt ccacggtgag ccctgcgcgc cattcgcttt cgggtttgag
    30001 attgatcacg gtctcgacca tctcggtcgg cgcggggtcg gtcgcggtcg cggcgcgccc
    30061 cgccttgccg aagaccgact ccacctccgg gaaggatttg atgatcttgt cctgggtctg
    30121 gagaagctcg gccgtcttgg tcacggaaat gcccggcaag gtcacgggca tgtacatcag
    30181 cgtgccctcg ttgaggttgg gcatgaactc gctgccgagc ctcgatgcgg gccagaggct
    30241 caacgcgagc acggccagcg cgatcgcgat ggtggcgccc ttgaatttga gcgcgagctc
    30301 gatggccgga cggtagatcc agatcaagaa ccggttcacc gggttcttgt tctccggaat
    30361 gatgcggccg cggacgaaga gggtcatcag cgccgggacc agcaggatcg acagcagcgc
    30421 cgcggcggcc atggcgaagg tcttggtgta agcgagcggc ttgaacagcc ttccctcctg
    30481 cgcttccagc gcgaagatcg gcaggaagga cacggtgatg atgagcaggc tgaagaagag
    30541 cgcggggccg acttcgatgg cggcctccac cacgatttca tgccgcttct tgccgggcgg
    30601 ggcgcgttcc agccgcttat gcgcgttctc gatcatgacg atggcggcat ccaccatcgc
    30661 gccgatggcg atggcaatgc cgccaagact catgatgttg gagctgaggc ccatggccca
    30721 catgcagata taggcgatga gaatgccaag cggcagcgtg atgatggcga cgagcgcgct
    30781 gcgcgcatgc agcaggaaga tcacgcagac caaagcgacg atcaggctct cctcgatgag
    30841 cgtgcgcttc agcgtatcga tggcgcggta gatcagggcc gagcggtcgt agaccgcctc
    30901 gacggtgacg ccttcgggca ggctcggcgc gatttcggca aggcgctgct tgacgttttg
    30961 gatcaccgac agcgcgttct cgccgaagcg ctggagcgcg atgcccgaaa cgacctcgcc
    31021 ttcgccatcc aattcggcga tgccgcggcg ttcgtccgcg gtgagctcga cgcgcgccac
    31081 gtccttcaat tgtacgggca cgccgccctc gttcttgacc acgatttgct cgaggtccga
    31141 caggctcttg atatagccgc gaccgcgcac ggcgaactcg gtctcggaga cctcgaccgt
    31201 gcggccgccc acatccatgt tgcttgcccg gatcacgtcg cggatgcgcg agagcggaat
    31261 gccgagcgcc tgcaggcgcc gcgggtcgat caccacgctg tattgcttga cgaagccgcc
    31321 cacgcttgcg acttccgcca cgcctgacgc attcgagagg ccgaagcgga tgtaccagtc
    31381 ctggagggtg cgcagctcgg ctaggcttcg ttgcgcgcct cgcaccacat attgatagac
    31441 ccaccccacc cccgtggcgt cgggcccgag gctcggggtc acgccctgcg gcaggcgccg
    31501 ggcggcggag ctcagatatt cgagcacgcg cgatcgcgcc caataaacgt cggttccgtc
    31561 ctcgaagatc acatagacaa acgagacgcc gaagaacgaa aagccgcgca cgacgcggga
    31621 tttgggcacg gtcagcatcg cggtggtcag cggataagtg acctgatcct cgacgacttg
    31681 cggcgcctgg cccggaaatt cggtgtagac gataacttgc gcgtcggaga ggtcggggat
    31741 ggcgtcgagc ggcacgcgcg acacggcgtg gatgccgagc gcggtcacga agaccgtagc
    31801 gagcccgacg agaagcacat tgcgtgcgga ccagcggatg atggcggcaa tcatggctgc
    31861 gctccttggc taaaagcctg gagcgccgcc ttgagattgc tttcggcatc gatgaggaag
    31921 gtggccgagg tgaccacctc ttcgccaagc tccaggcccg acgtgacttc ggtataacct
    31981 tcgccgcgcc gcccgagctt gacttcgcgc ggctcgaacc gtccctcgcc cttggccacc
    32041 aagacgatct ggcgcgtgcc gctgtcgatg atggcactat cgggaatcgc ggtcgccggc
    32101 gcatcgccct cgccggcccg aaacagcaca tcggcgtaca ttccggtcct gatgttgccg
    32161 tcggggttcg gcaactctat ccggacggag atcgtgcgcg tctcaggctt catcatttcc
    32221 gggtagatga agctcaccac gccttcgtgg ggctcgccgg cagatgcgcg gagcgttacc
    32281 gtcgccgggc tccccacctt gatcgcggcg atatcagcct cggcgacttc ggccacaacc
    32341 catacgttgg cgtggtcggc aatgcggaac agctcatctc cggccgcgac gcgctgacct
    32401 tcgatgacct ttttctcgat gacgtcgccc gtcgcgggcg aggcccagtc gatagtgcgc
    32461 gggttggtca ggctgttgcg cacctcgtcg atgcgactgt ccgggacgcc gagattgcgc
    32521 agacgttgta tggcgcccgc aacgtttcgg ctcgtatcgg acttcgcgga ggcgccttcc
    32581 gcgcgcatcg ccacgacgag atcggcctgc gcgagctgga tttgcggact gtagaagcgg
    32641 aacagaggtt cgccctcgcg gacatgctgg ccggtcttgt tgacgaacag atcctcgacg
    32701 ta
//
LOCUS       DASUQP010000024         4990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1238135, whole genome shotgun sequence.
ACCESSION   DASUQP010000024 DASUQP010000000
VERSION     DASUQP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238135"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..2003
                     /gene="gatB"
                     /locus_tag="VFY74_01210"
     CDS             531..2003
                     /gene="gatB"
                     /locus_tag="VFY74_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5930358.1"
                     /translation="MDTKTDKSKLIEGATGPWEVVIGMEVHAQVTSNAKLFSGASTEF
                     GGEPNSHVSLVDAAMPGMLPVINEGCIAQAVRTGLGLKAKINLNSIFDRKNYFYPDLP
                     QGYQISQYKSPVVGEGEVVVDLPGGERVTVGIERLHLEQDAGKSLHDQHPHHSYVDLN
                     RSGVALMEIVSKPDLRSAEQAKAYIGKLRTIMRYLGTCDGNMEEGSLRADVNVSVRRP
                     GEPFGTRCEIKNVNSIRFIGQAIEHEARRQIDILEEGGAIQQETRLFDAKTGETRSMR
                     SKEEAHDYRYFPDPDLLPLELTQDYIDGLAHDLPELPDQKRARFIAAYGLSPYDADVL
                     IAEKESADYFEAVAKGREAKQAANWVVNELFGRLNKEGRSIADSPVTADQLGSVLDLI
                     KSGTISGKIAKDVFDIVWAEGGDPAAIVAARGLTQVTDAGAIEQAVDEVIAANPEKAE
                     QAKAKPALAGWFVGQVMKATGGKASPQAASELIKKKLGLE"
     gene            2000..2974
                     /locus_tag="VFY74_01215"
     CDS             2000..2974
                     /locus_tag="VFY74_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX5930359.1"
                     /translation="MSHAIRIHEYGGPEVLKWEKTEAGAPGPAQVKIKQTAVGLNYID
                     VYMRTGLYPQSAFPFIPGMEGAGVVTEVGEGVRDLKAGRRVAYAGLAGAYAEERVIPA
                     DRVVKIPAGISDRIAAAIMLKGMTAQYLLRRTYKVGPGTTLLFHAAAGGVGLIACQWA
                     ASLGATVIGTVGSAGKALIARAHGCAHVINIREEDLVAKVRTYTKGKGVDVVYDSIGK
                     DTFPASLDCLKPLGMWVSFGQASGPVPPFNITLLSQRGSLFATRPSLASYIDTRKDLV
                     ATANELFDVVAKGKVKIAVNQTYPLAEAAQAQRDLEARLTTGSTVLLA"
     gene            3285..3539
                     /locus_tag="VFY74_01220"
     CDS             3285..3539
                     /locus_tag="VFY74_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930360.1"
                     /translation="MELSIETGLGAQTGTPDALFELGMLYATGRDVAADLVAAHKWFN
                     LAAARGNAAAMKRRVELAREMSAEQIAEAQRLAREWLQTN"
     gene            complement(3568..4203)
                     /locus_tag="VFY74_01225"
     CDS             complement(3568..4203)
                     /locus_tag="VFY74_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930361.1"
                     /translation="MNLPACRTYVAGLAACLFAAAPEPASSADASYCITCKNPDQTYL
                     CRVTAGGARPSDALKLYCVMRTAKEGNHASCSAVRSPSCNGIEKVYSYDGPIPEDLAS
                     DVRKVRERIQQEQKAFEKPNSDGPDTLVELTGRARERLRNARGALRGRSEPDQPLPAD
                     PLPKNETATPLSADASPTADTAERPGLARRSYRCVMSLFRNCRSETAGQGQ"
     gene            complement(4407..>4990)
                     /locus_tag="VFY74_01230"
     CDS             complement(4407..>4990)
                     /locus_tag="VFY74_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5930362.1"
                     /translation="QPASDPPLTEAPAPLAAPPSTAEQPRIAEQPKIAEQPKITRKLK
                     SDANIRSAPSMSAEIIGSAAAGAELHVTAYEADWVQFVDPSSGNKGWIHSTSIESENA
                     GDVAIAATDPVDELPPVEAAKPKSEKSGKPRAKGNIVSKRQRPIDPAPKAYVELPPDE
                     DFLPARRRERPGILERRRMLREGLMSPGFLPPR"
BASE COUNT          953 a         1492 c         1623 g          922 t
ORIGIN      
        1 cgaggaatag aagctccgcc agcagctcga aaacgaaaga tagtgttgca ggctgctcac
       61 tcgttgtgag gcctgggcgc taactcccct ctcccctgtg gggagagggc agggtgaggg
      121 ggatgaagac tcgaaccagt ctggcacgga atctgcgcag cgagtcaacg catgctgaac
      181 ggaagctctg gtccagattg cgcgaccggc gtttcctcgg tctcaagttc aagcgccaag
      241 ttccgatcga tcagttcgtc gtcgattttg cctgcgtgga ggcaaaactc attatcgagg
      301 tcgacggagg ccagcacgac aaacggagtg ctgccgacag agggcgaacc gagatacttg
      361 aatcggtcgg atatttggtg ctgcggttct ggaacaatga cgtgcttaag aacaccgacg
      421 gcgtgcttga gttcattgct ttaacaatca aacgtgaggc gttagaaccc ctcacccggc
      481 gcgcaagggc gcgccgacct ctcccaaagg ggagagggtt aggctctgca atggatacta
      541 agacagacaa gtccaagctc atcgaagggg ccaccggtcc gtgggaggtc gtgatcggca
      601 tggaggtgca tgcccaggtc acctccaacg ccaagctgtt ctcaggcgct tcgacggagt
      661 tcgggggcga gccgaattct catgtgagcc tggtcgatgc cgccatgccc ggcatgttgc
      721 cggtgatcaa cgaaggctgt atcgcgcaag ccgtccgaac cgggctgggg ctcaaggcca
      781 agatcaacct caactccatc ttcgaccgca agaactactt ctatcccgat ctgccgcaag
      841 ggtatcagat cagccagtac aagagccccg tcgtcggcga gggcgaggtc gtcgtcgatc
      901 ttccgggcgg cgagcgggtg acggtgggca tcgagcggct gcatctcgag caggatgcgg
      961 gcaagagcct gcacgaccag cacccgcacc attcctatgt tgacctgaac cggtcgggcg
     1021 tcgcgctgat ggaaatcgtg tcgaagcccg acctccgctc ggcggagcag gccaaggcct
     1081 atatcggcaa gctccgcacc atcatgcgct atctcggcac ctgcgacggc aacatggaag
     1141 aaggctcgct ccgcgccgac gtgaatgtgt cggtgcgccg cccgggcgag cccttcggca
     1201 cgcgatgcga aatcaagaac gtcaactcga tccgcttcat cggccaggcc atcgaacatg
     1261 aggcaaggcg tcaaatcgac attttggagg agggcggtgc gatccagcag gagacgcgcc
     1321 tgttcgatgc caagaccggc gagacgcgct ccatgcgctc caaggaggag gcgcacgact
     1381 accgctattt ccccgaccct gacctcttgc cgctcgaatt gacgcaagat tacatcgacg
     1441 gtctcgcgca cgatctgccc gagcttcccg accagaagcg ggcgcgcttc atcgccgcgt
     1501 atgggcttag cccttacgat gccgatgtgc tcatcgccga gaaggaatcg gccgactatt
     1561 tcgaggccgt cgcgaagggg cgcgaagcca agcaagcggc gaactgggtc gtcaatgagc
     1621 tgttcggacg gctcaacaaa gagggacgga gcattgccga cagtccggtc acggcggacc
     1681 agctcggaag cgtcctcgat ctcataaaat ccggcacgat ttccggcaag atcgccaagg
     1741 acgtgttcga catcgtctgg gccgagggcg gggatcctgc ggcaatcgtc gcggcgcgcg
     1801 gcctgacgca ggtcaccgat gccggcgcta tcgagcaggc cgtcgatgag gtcatcgccg
     1861 ccaatcccga gaaggccgaa caggcgaaag cgaagcccgc gctcgcgggc tggttcgtcg
     1921 ggcaggtcat gaaggcgacg gggggcaagg cgagccctca agccgcaagt gagctcatca
     1981 agaagaagct cgggcttgaa tgagccacgc gatccgcatt cacgaatatg ggggccccga
     2041 agtcctcaaa tgggagaaga ccgaggccgg agcaccgggc cccgcccagg tcaagatcaa
     2101 gcaaacggcg gtcggcctca actatatcga cgtctatatg cgcacgggtc tttacccgca
     2161 aagcgcgttt cccttcatcc ccgggatgga aggcgcgggc gtcgtcaccg aagtgggcga
     2221 gggggtaaga gacctgaagg ccggtcgccg tgtcgcctat gcggggctgg ccggcgccta
     2281 tgccgaggag cgcgtgatcc cggccgaccg cgtggtcaag atcccggcag ggatcagcga
     2341 ccggattgcg gccgccatca tgctcaaggg catgaccgcg caatacctgt tgcgccgcac
     2401 ttataaagtc ggccccggaa ccacgcttct gttccatgcc gccgccggcg gtgtcgggct
     2461 catcgcctgc cagtgggcgg catctctcgg cgcgacggtg atcgggaccg tcggctcggc
     2521 cggcaaggct ctgattgcgc gggcgcacgg ctgcgcccat gtcatcaaca ttcgcgaaga
     2581 ggacttggtc gcgaaggtca ggacgtacac caagggcaag ggggtcgatg tggtgtacga
     2641 ctcgatcggc aaggacacgt tccccgcctc gctcgattgc ctcaagccgc tcggcatgtg
     2701 ggtgagcttc gggcaagctt cggggccggt gccgccattc aatatcacgc tcctttcgca
     2761 gaggggctcg ctgttcgcca cgcgtccgtc gctcgcgagc tatatcgata cgcggaagga
     2821 tctcgtggcg acggcgaacg agctgttcga cgtcgtcgcc aagggcaagg tcaagatcgc
     2881 cgtcaaccaa acctatccct tggccgaagc agcccaagcg cagcgtgacc tcgaagcccg
     2941 gctcacgacc ggatcgacgg tgttgttggc ctgaccgccc ccgaaagcga atctcccaaa
     3001 tcgattgttg gttaatcgcc gctgacgtcg attggtgaag ggatcgttgc cggagccaaa
     3061 gcttaacgcg acctgcgggc gagacacgtg tggcgtaaac caatgttacc gctcgttatg
     3121 aactcggtga tttcacgtta agcatgcgac tttagacgtt gttgaaaatc ctctcccatc
     3181 attctcgcgt ggatgggtcg agatgacgtc tgacagcgcc gagataatcg acaaatagtc
     3241 ggcgggcttt cagaccgaag aggggggata aacgcgtggc acgaatggag ctttcgatcg
     3301 agacgggcct cggcgcccag acgggcacgc cggacgcgct gttcgaactg gggatgcttt
     3361 acgcaacggg tcgggacgtg gcggccgatc tcgtggcggc gcacaaatgg ttcaatctcg
     3421 cggcggcgcg cggcaacgcg gcggccatga aacgtcgcgt cgagctggcc cgcgagatgt
     3481 cggctgagca gatcgccgaa gcgcagaggc tcgcccgcga gtggctgcaa acgaattgag
     3541 cctgcgcggg gcaggcgcct cgtgggccta ttgaccctgg cccgcggttt cgctgcggca
     3601 attgcggaag agggacatca cgcagcgata gcttcgccgc gccaagcctg ggcgctcggc
     3661 ggtatcggcc gttgggctcg cgtccgcgct aagcggtgtg gctgtttcgt ttttcggtaa
     3721 cgggtcggcc ggcagcggct gatcgggctc ggaccggccc ctcagcgcac cgcgcgcatt
     3781 gcgtaagcgc tcccgcgcgc gccccgtcaa ttccacgagc gtatcgggcc cgtcgctgtt
     3841 gggcttttca aatgccttct gctcctgttg gatcctttcc cgcaccttgc gcacatccga
     3901 cgcgaggtcc tcggggatcg gtccgtcata gctatagacc ttctcgatgc cgttgcagga
     3961 cgggctgcgg accgccgagc aggaggcgtg gttgccctcc ttggcggtgc gcatcacgca
     4021 gtaaagcttg agcgcgtcgc tcggcctggc ccctccggcg gtgacccggc aaagataggt
     4081 ctgatccgga tttttacagg ttatgcaata acttgcgtcg gcggaggagg cgggctccgg
     4141 ggccgcggcg aagagacatg cggcaagccc cgcgacatat gtccggcagg cgggcaaatt
     4201 cattggcgcg cgggctccct cttcccgatt gcccaaaata ctagcatagg cttggtgcca
     4261 tggttgggcg gaaaatgggg aatattttgt catcatttcc gcagcatgcg cctcgccgcg
     4321 aatggcggcc tcgatgtcag gcacgccggg cgccgtccta tagcgagaaa gttgggggct
     4381 tgccgctagc gcggcgcctt cgggccttat cgcggcggta ggaagccggg cgacatcaat
     4441 ccttctcgca gcattcgccg cctttcaagg atgccgggtc gttcgcgccg tctggccgga
     4501 aggaaatctt catccggggg cagttcgaca taggccttcg gcgccgggtc aatgggtcgc
     4561 tggcgcttgg aaacgatgtt acctttcgca cgcggcttcc cggatttttc ggatttcggt
     4621 ttcgccgcct cgaccggagg cagctcgtct accgggtccg tggctgcaat agccacgtcc
     4681 ccggcattct ccgactctat cgatgtcgaa tgaatccacc ccttgtttcc cgagcttgga
     4741 tcaacgaatt gcacccagtc ggcctcgtac gccgtgacat ggagttcggc acccgccgcg
     4801 gcggaaccaa tgatttcggc ggacatcgag ggcgcgctgc gtatgttcgc atccgatttt
     4861 agtttgcgcg ttattttggg ctgctcagca attttgggct gttcagcaat tctgggctgc
     4921 tcagcagttg aaggtggcgc ggctagcggg gcgggcgctt cggtcaaggg tggatccgaa
     4981 gcaggttgat
//
LOCUS       DASUQP010000025         7212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1919845, whole genome shotgun sequence.
ACCESSION   DASUQP010000025 DASUQP010000000
VERSION     DASUQP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919845"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..580
                     /locus_tag="VFY74_01235"
     CDS             77..580
                     /locus_tag="VFY74_01235"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX5930363.1"
                     /translation="MDRAVRAACIAAALVATPALAQEVTLLEKFKDWTAYAATGTPKV
                     CFAVAKPTESNPKKIKRGPILFYISQWPADKVVNEISVKMGYPFGEGAKSTVTIGANK
                     FDMFTKDEGAFVEKPEMETKLLEAMKTANTMKIEGKSVRGTATTDVYSLNGLSDALDR
                     AAKECSG"
     gene            676..1854
                     /gene="rlmN"
                     /locus_tag="VFY74_01240"
     CDS             676..1854
                     /gene="rlmN"
                     /locus_tag="VFY74_01240"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5930364.1"
                     /translation="MTMTDLSLTRSGPAAGDRSHREPILGLDRSALAARLGALGVPER
                     QRRMRVSQLWSWAYVRGATDFAAMTDIANELRGELAASFSLARPEIVTEQVSIDGTRK
                     WLLRLEDGKEIETVYIPEEDRGTLCISSQVGCTLTCSFCYTGTQRLVRNLTAGEIVSQ
                     IALARDRLGDWPGAVAPDPRGLPDAMRKITNVVLMGMGEPLYNFDNVRDACLVAADGE
                     GLSVSKRRITLSTSGVVPEIPRWGEEAGTMLAISLHAARDDLRDTLVPINKKWPIKDL
                     LGACRAYPGLSNAKRITFEYVMLKGVNDSIADAKELVRLLAKIPAKINLIPFNPWPGA
                     PYECSDWEQIERFAEVVNRAGYASPVRSPRGRDIMAACGQLKSASVKQRAGARLAAPL
                     "
     gene            complement(1851..2447)
                     /locus_tag="VFY74_01245"
     CDS             complement(1851..2447)
                     /locus_tag="VFY74_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX5930365.1"
                     /translation="MAEIEFWYEFGSTYSYPAAMRIERLAADAGVALRWRPFLLGPIL
                     KQQGLNDSPFNIFAVKGRYMWRDLDRICQADGLALTLPPIRFPQNGLKAARLALIGEP
                     QGWTPAFSRAVFVANYAEQKDISEDATLRTILSALDVDTAAAFAAADTPENKEALKRQ
                     TEEAAARGLFGAPSFTIGDELFWGNDRLEAALAFAQRA"
     gene            2601..2963
                     /locus_tag="VFY74_01250"
     CDS             2601..2963
                     /locus_tag="VFY74_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930366.1"
                     /translation="MLFALGAIWAGDELRAAAPDDHLIRDGAPIFGMVLFAGTVGPAL
                     TALPALIAVVVGEVMRIRAWMYYVLAGGLSLAAIPILAAPETAELPAILTSHAMTIFA
                     TAGFAGGVLYWLLAGARA"
     gene            complement(2960..3205)
                     /locus_tag="VFY74_01255"
     CDS             complement(2960..3205)
                     /locus_tag="VFY74_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930367.1"
                     /translation="MIPQNLDKSPIDYRELKGRKFAGGLMARLSQQHGSEAWRKTVTR
                     HVPESWPAARLALQARIAALDVSQDETVLELTQEEAA"
     gene            3386..4609
                     /locus_tag="VFY74_01260"
     CDS             3386..4609
                     /locus_tag="VFY74_01260"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5930368.1"
                     /translation="MTRRKDVKKVVLAYSGGLDTSIILKWLQATYGCEVVTFTADLGQ
                     GEELAPARKKAEMLGIKEIFVEDLRETFVRDFVFPMFRANALYEGVYLLGTSIARPLI
                     AKRQIEIAKLTGADAVAHGATGKGNDQVRFELTYYALNPDIKVIAPWREWELKSRSDL
                     IAFAERHQIPVAKDKRGEAPFSVDANLLHSSSEGKVLEDPGAEPPPYVYQRTISPEEA
                     PDKATTVTIGFRRGDPVSIDGKALSPDALLARLNELGGENGVGRVDLVENRFVGMKSR
                     GVYETPGGTILLAAHRAIESLTLDREAMHLKDSLMSRYAELVYYGFWFSPEREMLQAL
                     VDKSQEHVEGEVTLKLYKGNVTVVGRSSPKSLYSDKMVTFEDDAGAYDQKDAEGFIKL
                     NALRLRLLGKRRDLK"
     gene            4613..5419
                     /locus_tag="VFY74_01265"
     CDS             4613..5419
                     /locus_tag="VFY74_01265"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX5930369.1"
                     /translation="MGHVLGIDAAWTVTNPSGVALLTTDTNPPRVLQASPSFEDFVRK
                     TMPDAWCGKYDAKASLADVLVAAKEMGADAIDVIAVDMPLAHQPVRGRRRCDQLISRS
                     FGARGCATHTPTTSRPGIVSDSLLREARQAGFNLVTEASHRPERALLEVYPHVALLEL
                     CNAGYRVPYKLARRARYWPEASAADRLSTILVSWERILRRLNERLDFDFAVDCTDRPL
                     QFWKAWEDVIDAIVCCWVGLEWLSGRARPYGDEIAAIWVPERTDLGQELA"
     gene            5400..6479
                     /locus_tag="VFY74_01270"
     CDS             5400..6479
                     /locus_tag="VFY74_01270"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX5930370.1"
                     /translation="MAKSWPDTRIIDLFGVELPIVLAPMANFAGLELAVAVAEAGGLG
                     SVPCAALDPGQIRTDVAAFRARARKPVSLNFSCHAGAPSDADKERAWLRHLAPYYAEL
                     GVEAPSLPFRAPHPPFDEAACAAIESTRPEIVSFHYGLPAPSLLGRVKAKGCKIMSSA
                     TTLREAQFLATHGADAIIAQGAEAGGHRGMFLETEVATQVGTLALVRAVTASVRVPVI
                     AAGGIADGRAIAAALALGASAVQIGTAYLMCHESTTSTLHKAALADPARETVITNVLT
                     GGAARGIVNRFIREQGPMNKAAPNYPLARLAIAPLRTKTEAVGQTDFSPLWSGQAARL
                     PGPIGARELTLQLAEETREALRGVA"
     gene            6705..>7212
                     /locus_tag="VFY74_01275"
     CDS             6705..>7212
                     /locus_tag="VFY74_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5930371.1"
                     /translation="MRWSLRIGSVGGTAILIHVTFLLFLVWLGAVYYRQGGIEAAWQG
                     TLFIVLIFLCVLLHELGHVFAARRYGVKTRDVTLWPFGGIANMERMPDKPSEELVVAL
                     AGPAVNVVIAAVLLLWLGPQLDVENLTRIEDPAVSLAAKVAGANIILVLFNMIPAFPM
                     DGGRVLRAL"
BASE COUNT         1257 a         2303 c         2328 g         1324 t
ORIGIN      
        1 ggttgaccgg ttggtttagg cgaaggagaa tcaagggccg ggggttggac cctgctcgtt
       61 gatcgggggt ggaaacatgg accgagccgt tcgggccgct tgcattgccg cggcgctggt
      121 cgcaacgccg gcgctggcgc aagaggttac gcttctcgaa aagttcaagg actggaccgc
      181 ctacgccgcg accggcaccc ccaaagtctg cttcgcggtt gccaagccga ccgagtcgaa
      241 tcccaagaag atcaagcgcg ggcccatcct gttctacatc tcgcaatggc cggccgacaa
      301 agtcgtgaac gagatcagcg tgaagatggg ctatcccttc ggcgaggggg cgaagtccac
      361 ggtgacgatc ggcgcgaaca agttcgatat gttcaccaag gatgaaggcg ccttcgtcga
      421 gaagccggag atggagacga agctgctcga ggccatgaag actgccaaca cgatgaagat
      481 cgaagggaag tcggtccgcg gcaccgcgac caccgacgtc tattcgctga acggcttgtc
      541 ggacgctttg gaccgcgccg ccaaggagtg cagcggctag ggtcctgccg ggcggtcgcc
      601 ctggccagaa cttgccagat caagcccggg acatgatatg accgtggcgc cggatcgtgc
      661 cggcgctctg atgtcatgac gatgacggat ctttcattga cccgaagcgg gcccgccgcg
      721 ggcgatcggt ctcaccgcga gccgatcctc gggcttgacc gcagcgcgct tgcagcgcgt
      781 ctcggcgctc tgggtgttcc cgagcggcag aggcgcatgc gcgtgagcca gctgtggagc
      841 tgggcctatg tgcgcggcgc aaccgacttc gccgccatga ccgacatcgc caacgagctc
      901 cgcggcgagc ttgccgcatc gttttcgctc gcccggcccg agatcgtgac cgagcaagtg
      961 tcgatcgacg gcacccgcaa atggctgctt cggctcgaag acggcaagga gatcgagacg
     1021 gtctacatcc ccgaggagga tcgcggcacg ctctgcattt cgagccaggt cggctgcacg
     1081 ctcacctgct ccttctgtta caccgggacc cagcgtctcg tgcgcaacct gacggcgggc
     1141 gagatcgtca gccagatcgc gcttgcccgc gaccggctcg gcgactggcc gggggcggtg
     1201 gcgccggacc cgcgcggact gccggacgcg atgcgcaaga tcacaaatgt cgtgctcatg
     1261 ggcatgggcg aacccctcta caatttcgac aatgtgcgcg acgcctgtct cgtcgccgcc
     1321 gacggcgagg gtctctccgt ctcaaagcgc cgcatcacgc tctcgacctc gggcgtggtg
     1381 cccgagattc cgcgctgggg cgaggaagcc ggcaccatgc ttgcgatttc gctgcatgcg
     1441 gcgcgcgacg atctccgcga cacgctcgtg ccgatcaaca agaaatggcc gatcaaggat
     1501 ttgcttggag cttgccgcgc gtatccgggc ctctccaacg ccaaacgcat caccttcgaa
     1561 tatgtgatgc tgaaaggcgt caacgattcg atcgcggacg cgaaggagct ggtgcggctg
     1621 ctcgccaaga tccccgccaa gatcaacctg atcccattca atccttggcc cggcgcgccc
     1681 tatgagtgct cggactggga gcagatcgag agattcgccg aagtggtgaa cagggcgggc
     1741 tatgcgagcc cggtgcggag cccgcgcggc cgcgacatca tggcggcctg cggccaattg
     1801 aaaagcgcaa gcgtgaagca gcgcgcgggc gcccggcttg cagcgccgct ctaggcgcgt
     1861 tgcgcaaagg caagcgccgc ctcgaggcga tcgttgcccc agaacaattc atcgccgatg
     1921 gtgaaagacg gcgcgccgaa caggccgcgc gcggcagcct cctcggtctg acgcttcaga
     1981 gcctccttgt tttccggcgt gtcggccgcg gcaaaggcgg ccgccgtgtc gacgtcgagc
     2041 gcagagagaa tcgtgcgcag cgtcgcgtcc tcgctgatgt ccttctgctc ggcgtaattc
     2101 gcgacgaaga cagctcggct gaaggcgggg gtccagcctt ggggctcgcc gatcagcgca
     2161 agccgcgcgg ccttgagccc attctgcggg aagcggatcg gggggagggt gagcgccaag
     2221 ccgtcagcct ggcaaatgcg gtcaagatcg cgccacatat agcgcccctt gaccgcgaag
     2281 atgttgaacg gcgagtcgtt caggccctgc tgtttcagga tcggcccaag caggaagggg
     2341 cgccagcgca gagcgacgcc ggcatcggcg gcgagccgct cgatgcgcat ggcagcagga
     2401 taggaatagg tgctgccgaa ctcgtaccag aactcgattt ccgccattgc ccaccttacg
     2461 atcgtggtgc cggtgttata aagctttccg gctccgaggg gagcgtgaca cgcaggcggc
     2521 acaagtgatt tcccgcatca tcttccgtgc aatctgggta atcgtggcct tctccattgc
     2581 cgcggtcggg ggcgctcgcc gtgctgtttg cgctgggtgc gatctgggca ggcgacgagc
     2641 ttcgcgccgc agcgccggat gatcacctaa ttcgggacgg cgcgccgatc ttcggcatgg
     2701 tgctgttcgc gggcaccgtc ggtccggctc tgaccgcgct cccggcgctc atcgccgtgg
     2761 tcgtaggcga agtcatgcgg atccgggcgt ggatgtatta cgtgttggcg ggcgggctat
     2821 cgcttgccgc gatccccatc ctggcggcgc ccgagacggc cgagctcccg gcgatcctca
     2881 ccagccatgc catgacgatc ttcgccacgg cggggttcgc cggcggggtt ctctattggc
     2941 tgctcgccgg cgcgagggct taggcggctt cttcttgcgt aagttcgagg accgtctcgt
     3001 cttgcgagac gtcgagggcg gctatccgcg cttggagcgc aagccgcgcc gcaggccaac
     3061 tctccggaac gtgccgcgtc acggtcttcc gccatgcctc gctgccgtgc tgttgcgaga
     3121 gccgggccat caaaccaccg gcgaatttgc ggcctttcag ctcgcggtag tcaatcgggg
     3181 atttgtcgag gttctggggg atcattggtt tagccctctc ttttactgcc gagcgaccct
     3241 cttcttctca cccggggcgc ttgccttcgg cgcttaacgc ttccttgcca acgaacgcag
     3301 atacccacct caaagttccg ctgtcccgcc ttgctccctc ccgcctcttc cctatagttg
     3361 cgcctccatt cgccccgaga accccatgac caggcgcaaa gatgtgaaaa aggtggtgct
     3421 cgcctactcc ggcgggctcg acacctctat catcctgaaa tggcttcagg caacctacgg
     3481 atgcgaggtt gtgaccttca cggccgatct cggccagggc gaggagctcg cgccagcgcg
     3541 caagaaggcc gagatgctcg gcatcaagga gatcttcgtc gaggacctcc gcgagacgtt
     3601 cgtgcgcgac ttcgtcttcc cgatgttccg cgccaacgcg ctctatgagg gggtctatct
     3661 gctcggcacc tcgatcgcgc ggcccttgat cgccaagcgg cagatcgaga tcgccaagct
     3721 gaccggcgcc gacgccgtcg cccatggcgc gacgggcaag ggcaacgacc aggtgcgctt
     3781 cgagctcacc tactacgcgc tcaatcccga tatcaaggtg atcgcgccgt ggcgggaatg
     3841 ggagttgaaa tcgcggagcg acctgatcgc gttcgcggag cggcaccaga ttccggtcgc
     3901 caaggataag cggggcgagg cgccgttctc ggtcgatgcc aacctgttgc actcttcatc
     3961 ggaagggaag gtgctcgagg acccgggcgc ggaaccaccc ccttacgtct atcaacgcac
     4021 gatcagtccc gaagaggcac ccgacaaggc gacaaccgtc acgatcggtt tcagacgcgg
     4081 cgatccggtc tcgatcgacg gcaaggcgct ttcgcctgac gcgttgctcg cgcggctcaa
     4141 cgagctcggc ggcgagaacg gcgtcggccg cgtcgatctg gtcgagaacc gtttcgtcgg
     4201 catgaagtca cgcggcgtct atgagacgcc cggcggcacg atcctccttg cggcgcaccg
     4261 ggcgatcgaa tcgctgacac tcgatcgcga ggcgatgcat ctcaaggaca gcttgatgtc
     4321 gcgctacgcc gagctcgtct attacggctt ctggttctcg ccggagcggg agatgttgca
     4381 ggcgctggtc gacaagagcc aggagcatgt cgaaggcgaa gtcacgctca agctctacaa
     4441 gggcaacgtc acggtcgtcg gccggtcgag cccgaaatcg ctctactccg acaagatggt
     4501 cacgttcgag gacgacgccg gcgcctatga ccagaaggac gccgagggct tcatcaagct
     4561 caacgcgctg cgcctgcgcc ttctcggcaa gcgccgcgat ttgaagtgaa gcatgggaca
     4621 tgtcctaggc atcgatgccg cctggacggt cacgaacccg agcggcgtag ctcttctcac
     4681 aaccgacacc aatcctccgc gtgtcctcca ggcctcgccc tcattcgagg atttcgtgcg
     4741 caagacaatg ccggacgctt ggtgtggcaa atatgacgcc aaagcttcgc tcgcggatgt
     4801 gttggtcgct gcgaaggaaa tgggcgccga tgcgatcgac gtcatcgcag tcgatatgcc
     4861 gcttgcgcac cagcctgtac ggggacgtcg ccgctgcgac caactgatct cacgcagctt
     4921 cggcgcgcga ggctgcgcga cacatacgcc gaccacctca aggcccggca tagtttcgga
     4981 cagtttgctg cgtgaggcac gacaagcggg ctttaacctc gtgaccgagg cttcacaccg
     5041 acccgaacgt gcgcttctag aagtctatcc ccatgtagct ctgcttgagc tttgtaacgc
     5101 cggttatagg gttccttata agctggcgcg ccgtgcgcgt tattggcctg aggcgagtgc
     5161 tgccgaccgg ctcagcacga tcctcgtgtc atgggagcgt atcctgcgcc gcttgaacga
     5221 aaggctcgat tttgactttg ccgtcgactg cacggaccgt ccgttgcaat tttggaaggc
     5281 ctgggaggac gtgatcgacg ccatcgtctg ctgctgggtt gggcttgaat ggctttccgg
     5341 tcgagcgcgg ccctatggcg acgaaatcgc ggcaatctgg gtgcctgaaa ggacagacct
     5401 tggccaagag ttggcctgat acgcggatca ttgatctttt tggcgtcgag ctgcccatcg
     5461 tgcttgcgcc gatggcgaac ttcgcgggcc tcgagctcgc ggttgccgtg gccgaagcgg
     5521 gcggtctcgg atcggttccc tgcgctgcgc tcgatccggg ccagatccgc accgatgtgg
     5581 ccgcgttcag agcgcgcgcg cgcaagcctg tgagcctcaa tttctcctgt catgccgggg
     5641 cgccatcaga cgccgacaag gagcgcgcct ggctgcggca ccttgcgccc tactacgccg
     5701 agcttggcgt cgaggcgccg tcgctgccgt tcagggctcc gcatccgccc ttcgacgagg
     5761 cggcttgcgc cgccatcgaa tcgacgcgcc ccgaaatcgt gagctttcac tacggcctgc
     5821 ctgcgccaag cctgctcggg cgcgtcaagg cgaaaggctg caagatcatg tcatcggcga
     5881 cgacgctccg cgaggcgcag tttctcgcca cgcacggcgc cgatgcgatc atcgcgcaag
     5941 gcgccgaagc gggcgggcat cgcggcatgt ttctcgagac ggaggtcgca acgcaagtgg
     6001 gaacgctggc gctggtgagg gccgtcaccg cctccgtgcg agtgccggtg atcgccgcgg
     6061 gcggaattgc cgacgggaga gccatcgctg cggcgctggc gctcggtgct tcggccgtgc
     6121 agatcggcac ggcctatctc atgtgccacg aatcaacgac ctcgacgttg cacaaggccg
     6181 ctctcgccga ccccgcccgc gagaccgtca tcaccaatgt cctcaccggg ggcgcagcgc
     6241 gcggaatcgt caaccgattc atccgcgagc aagggccgat gaacaaggcc gctcccaact
     6301 atccgctcgc acgccttgcc atcgcgccgc tgcggaccaa gacggaggcc gtgggccaga
     6361 cggatttttc gccgctgtgg tctgggcaag cggccaggct gccaggcccc atcggagcac
     6421 gggagttgac gctgcaactt gccgaggaaa cgcgcgaggc gcttcgcggg gtggcttagc
     6481 ctcgatcggg catgaccacc gcacccttga ggaatttctc gaagacgctt tccggcacgc
     6541 cgaaattggt ggaaccggtg ggagcgcaag cgcataaggg ctcaaggctg cccaatctgg
     6601 atcggaactg ctaacgaaga cttgccttcg actttctctc cgctagccgt tctgcgaccg
     6661 gtcgagacta cctatatgag cggacgaaac ttgaggctca ttgcatgcgc tggtcacttc
     6721 gcatcggatc ggtcggtgga acggcgatcc tgatccacgt caccttcctt ctgttcctcg
     6781 tctggcttgg cgctgtctac taccgccagg gcggaatcga ggcggcatgg caaggcacgc
     6841 tcttcatcgt gctcatcttt ctctgcgtgc tgctgcatga gctcggtcat gtcttcgccg
     6901 cccggcgcta tggcgtcaag acgcgcgacg tgacgctttg gccgtttggc ggcatcgcca
     6961 atatggagcg gatgccagac aagccgagcg aagagctggt cgtggcgctg gccgggcccg
     7021 cggtcaacgt ggtcatcgcc gccgtacttc tgctctggct cgggccgcag ctcgatgtcg
     7081 agaacctgac caggatcgag gatccggccg tgtctctcgc tgccaaggtg gccggcgcca
     7141 acatcattct cgttctgttc aacatgatcc cggccttccc catggatggc ggccgggtat
     7201 tgcgcgcgct tc
//
LOCUS       DASUQP010000026         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_11661,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000026 DASUQP010000000
VERSION     DASUQP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11661"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VFY74_01280"
     CDS             complement(<1..1323)
                     /locus_tag="VFY74_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5930372.1"
                     /translation="MTATRKILELNAFDMDAEAGLDPDVAELVTRRKRTFGPASMLFY
                     ARPLHLVRGEGVWLIGADGTRHLDAYNNVASVGHCHPRVVAAVSRQMAALNTHSRYLY
                     DIVYTYAERLLATLPREVSNIVFTCTGSESVDLSLRIARTVTGGTGFIVTANAYHGNT
                     LAVTQISPSSASAEPRSPHVFLVPAPDTFRTPSKDLGPLFAANVRKAIAAMKRKKIRF
                     AALVADSIFSSDGIYPGEPGFMAEAIASVREAGGVFIADEVQPGFARTGEKMWGFARH
                     GLVPDMVAMGKPMGNGYPIGGVAVKPELLATFGSTTGYFNTFGGNPVAAAAGLAVLET
                     IEAEKLQENARDTGAYLLAELESVADRFACVGDVRGKGLYIGVELVRDRKSKTPARDT
                     ATRIVDMLRDRNVLVGIAGPYGNVLKIRPPLSFQREHVDILVKALSDSL"
     gene            1725..>3292
                     /locus_tag="VFY74_01285"
     CDS             1725..>3292
                     /locus_tag="VFY74_01285"
                     /inference="COORDINATES: protein motif:HMM:NF011979.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HEX5930373.1"
                     /translation="MGAGGVEVMDSTGARDFDMIDTFIARYHIDPEIVPEAMGMSSHE
                     VARMLVDMNVPRSELVRLARGMTPAKLAEVVAELSSLEIAFAYSKMRARKTPGNQAHV
                     TNAKDDPLQMAADAATAVAFGFDEVETTMRVARNAWSNAVACAVGASVGRWGTLFQCS
                     SEEAEELRIGMAGFTSYAETVSVYGTEKSFIDGDDTPWSKAFLAAAYASRGIKMRCTS
                     GAGSELLMGFHEKKSLLYLEARCLCLQRAMGVQGTQNGGIDGAPITCTVPGGMRELMA
                     ENLIAVWLDLECASGNDARPTESEIRVGAKIMPFLIAGSDLICSGFGSILKYDNSFNP
                     SLLNGEELEDYLVLQRDFEADGGLTPVEETRVLALRTRAVEAISAVLEELGLGQPTED
                     MKASVVAASGSDETRSYLPRDVTLISESIKERAISVIDVIKALARRGFSEEAENLLNL
                     VRLRISGDYLQTSAVVRDGRVLSAINDPNDYLGPGTGYRVSKARRDEIVAIRDVLTRE
                     EVLRAESVHKGAES"
BASE COUNT          584 a         1034 c         1094 g          580 t
ORIGIN      
        1 gagcgagtcg gacagcgcct tgacgaggat gtcgacatgc tcccgctgaa aggagagcgg
       61 cgggcggatc ttcagcacgt tgccataggg gccggcaatg ccgaccagca cattgcggtc
      121 gcggagcatg tcaacgatgc gcgttgcggt gtccctggcg ggcgttttcg atttgcgatc
      181 gcgcacgagc tcgacgccga tgtaaaggcc ctttccgcgc acgtcgccga cgcaggcgaa
      241 gcggtccgcg acggactcga gctcagccaa gagataggcg ccggtatcgc gcgcgttctc
      301 ttgcagcttc tcggcctcga tcgtctccag gacggcaaga ccggccgcgg cggcgaccgg
      361 gtttccgccg aaggtgttga agtagccggt cgtcgacccg aatgtcgcaa gcagttccgg
      421 cttcaccgcg accccgccga tgggatagcc gttgcccatc ggctttccca tggcgaccat
      481 gtcggggacg aggccgtggc gcgcgaaccc ccacattttt tcgcccgtgc gcgcgaagcc
      541 cggctgcacc tcgtcggcaa tgaaaacgcc gcccgcttcg cgcacgctcg cgatggcttc
      601 ggccatgaac ccgggctcgc ccgggtagat gccgtcgctc gagaagatgc tgtcggcgac
      661 gagcgcggca aagcggattt tctttcgctt catggcggca atcgccttcc gcacattggc
      721 ggcgaagagc ggccccaaat ccttggacgg cgttcggaac gtgtcggggg ccgggaccag
      781 aaagacgtgc gggctgcgcg gctcggcgct cgccgatgag ggggagattt gcgtcacggc
      841 cagcgtattg ccgtgatagg cattcgccgt cacgatgaaa cccgtgccgc ccgtgaccgt
      901 gcgggcgatg cggaggctca agtcgacgct ttcgctcccc gtgcaggtga agacgatatt
      961 ggacacttcg cgcggcaacg tcgccagcaa ccgctccgca taggtgtaga caatgtcata
     1021 aaggtagcgg ctgtgcgtgt tgagggccgc catttgccgc gacacggcag cgacgacgcg
     1081 cgggtggcaa tgacccaccg acgccacgtt gttgtaggcg tcgaggtggc gcgttccgtc
     1141 ggcgccgatg agccagacgc cttcgccgcg gacgagatgg agcgggcgcg cgtagaacag
     1201 catcgatgcc ggcccgaacg tgcgcttacg gcgggtaacg agttccgcca cgtcgggatc
     1261 gaggccggct tccgcgtcca tgtcgaacgc gttgagttcg agaattttac gtgtcgctgt
     1321 catgacaccc ggcacaaact tgtagcgctc ctcagcgcct tacgcctcta gcctagatca
     1381 gaccttcgca accgtcttac ccgcgaagat agcatctgtt caatttttcc cgagcaccgg
     1441 gccaccgggg aggccagccg gcgctccctc cggtcaaggt gtggcgccgc ctcgtcaaat
     1501 ggcttcggcg ccgctgccct atcctctata gtcccgtgcc gttgacgtcg atccttgctt
     1561 cacgagagcc ccatggcagg agagccgggc acgttcgcca atcgctggaa acgcttctcc
     1621 gactgggacg agcgcccact tcggcttgac aaattcgcga tcgaggacgc ggagaacggc
     1681 tttgcgtttt tacgagccgt cacgatccga agcccggact gacactgggc gcgggcggcg
     1741 tcgaggtgat ggacagcacg ggcgctcgcg acttcgatat gatcgacacg ttcatcgccc
     1801 gctatcacat cgaccccgag atcgtgccgg aagcgatggg catgtcttcg cacgaggtcg
     1861 cgcgcatgct ggtcgacatg aacgtgccgc ggagcgagct cgtgcggctg gcccgcggca
     1921 tgacgccggc aaagctcgcc gaggtcgtcg ccgagctctc ttctctcgag atcgccttcg
     1981 cctatagcaa gatgcgcgcg cgcaagacgc ccggcaatca ggcgcatgtg accaatgcca
     2041 aggacgatcc cctgcaaatg gcggcggacg ctgcgaccgc ggtcgcattc ggatttgacg
     2101 aggtcgagac gacgatgcgc gtcgcgcgga acgcctggtc gaacgcggtg gcctgtgccg
     2161 tcggcgcttc cgtcggacgc tggggcacgc tgtttcagtg ctcgagcgag gaggcggagg
     2221 agctcaggat cggcatggcc ggctttacgt cttatgccga gaccgtctcc gtctacggca
     2281 ccgagaagag ctttatcgac ggcgacgaca cgccgtggtc gaaggccttt ctcgccgcgg
     2341 cctatgcctc gcgcggcatc aagatgcgct gcacctcggg ggcggggtcc gagctgctga
     2401 tggggttcca cgagaagaag tcgctgctct atcttgaggc gcgctgcctc tgtcttcagc
     2461 gcgccatggg tgtgcagggc acgcagaatg gcggcatcga cggggcaccg atcacatgca
     2521 ccgtgcctgg cggaatgcgc gagctgatgg cggagaatct gatcgccgtc tggctcgatc
     2581 tcgaatgcgc ctcgggcaat gacgcgcgtc cgaccgaatc ggagatccgc gtcggcgcca
     2641 agatcatgcc gtttctcatc gccggctcgg acctgatctg ctcgggcttc ggctcgatcc
     2701 tgaaatacga caattcgttc aatccatccc ttctgaacgg cgaggagctc gaagactatc
     2761 tcgtgctcca gcgcgatttc gaggcggatg gcgggctgac cccggtcgag gagacccgcg
     2821 tccttgcgct cagaacgcgc gccgtcgagg caatcagcgc cgtgctcgaa gagcttggcc
     2881 tcgggcagcc gacggaagac atgaaggcaa gcgtcgtcgc cgcatcggga tcggacgaaa
     2941 cgcgaagcta tcttcctcgc gacgtgacgc tgataagcga aagcatcaag gagcgcgcga
     3001 tcagcgtcat tgacgtgatc aaggcgctcg ccaggcgcgg cttcagcgag gaggcggaga
     3061 atcttctcaa cctcgtgcga ttgcggattt ccggcgacta tctgcagacc tcggcggtcg
     3121 tgcgtgacgg ccgtgtcctc agcgcgatca acgatccgaa cgattatctt ggccccggca
     3181 ccggataccg cgtgagcaag gcgcgccgcg acgagatcgt ggcgatccgc gacgtgctga
     3241 cgcgcgagga ggtgctgagg gccgagtccg tgcacaaggg cgccgagagc aa
//
LOCUS       DASUQP010000027         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1783349, whole genome shotgun sequence.
ACCESSION   DASUQP010000027 DASUQP010000000
VERSION     DASUQP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783349"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..2041
                     /locus_tag="VFY74_01290"
                     /pseudo
     CDS             470..2041
                     /locus_tag="VFY74_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018116964.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            2240..>2807
                     /locus_tag="VFY74_01295"
     CDS             2240..>2807
                     /locus_tag="VFY74_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5930374.1"
                     /translation="MFQVYLPIAEISVNLLVMLGLGAAVGFLSGMFGVGGGFLLTPLL
                     MFSGISAPIAVATGANQVVATSVSGALAQWRRGNIDFHMGAVLIAGGVVGALAGVFLL
                     KVLREAGQAGLIVSLTYVVLLGAVGTLMLGESLRALGRARAGTPLPARRSGQHNWIHR
                     LPLKLRFPRSRLYISAIPPLVIGAVVGLL"
BASE COUNT          524 a          895 c          882 g          506 t
ORIGIN      
        1 ggcgaaggac gatcggccgt tcgtcttgca gaaagccgga ggcctgcaag ggacgttctg
       61 ctacgtcgcc ttcgcgcccg agccggaacg cagccgtctt tatcgccatc aagaagttcg
      121 atttcggcgc ggctttgacc atgggcgagt ttgccaacga aatgctggag acgatcgcgc
      181 cgcgatagtt ggggcggacg ctagcacgca agttccgcac gcgcacctag gcacatgctc
      241 aaccggttgt agcagtcgtt acggcaatgc atgcgtgccg aaacgggacg aggctctatc
      301 gatgcggtcg atatcaacgc gatcgatgca ggccgatcgc aggtcaaggt cacagattca
      361 gttgtcgcgc ggagtgcatc ggcttatgac gaacacgtcc tatcgcgcgg gcattctgaa
      421 tgtcagaccc gcgcgttttc gtcgagccgc tatcgagagg gaggacgaaa tgtttgtaaa
      481 gagatccggc tggcagttcg ccttgctcgc gtgctgcatg attgcactcg gcctcggcct
      541 ggccggccaa gcgtccgcgc aaaccaagaa gcccaacatt ctggtgatct ggggcgacga
      601 catcgggggc ttcaacatca gcgcctataa ccagggcgtg atgggttaca agaccccgaa
      661 catcgacagc atcgccaggg acggcgccct cttcaccgac tggtatggcc agcaaagctg
      721 caccgccggc cgcgccgcct tcatcaccgg tcaatcgccg atccgcaccg gcttgaccaa
      781 ggtcggactc ccgggcgcgc cggaaggcat gaaagcggaa gacccgacca tcgccacgct
      841 gctcaagccg ctcggctacg cgacggggca gttcggcaag aaccatctcg gcgatcgcga
      901 cgagatgttg ccgacggctc atggcttcca cgaattcttc ggcaacctct accatctcaa
      961 tgccgaagag gagccggaaa acgtcgacta accgaaggat ccggagttca agaaaaagtt
     1021 cgggccgcgc ggcgtcatcc attcctgggc aaacgctgat ggaacgcaaa agatcgaaga
     1081 caccggcccg ctgacaaaga agcgcatgga aacggtggac gaggaggtca ccaaggccgc
     1141 gctcgctttc atcgacaagg cgcacaagga cggcaagccg ttcttcgtct ggtggaattc
     1201 cacccgcatg cacattttca cgcacctcaa gccggagtcc gaaggcaaga gcggccttgg
     1261 catctatgcc gacggcatgg tcgagcatga tggcatggtc ggtcagctgt tggacaagct
     1321 gaaggaactc ggcatcgagg acaacaccat tgtcatgtac tcgaccgaca acggcgccga
     1381 gaccttcacc tggcccgatg gcggcaccac gatgttccgc ggcgagaaga acacgaattg
     1441 ggaaggcggc taccgcgtgc ccgcgctgat ccgctggcct ggcacgatca aaccgggaac
     1501 catcgtcaac gacgtctgcg cgcacgagga catgctgacc acgctgctcg cggctgccgg
     1561 cgagccgaac gtcgcccagg agttgctcta gggcaagcag gtcggcgaca ggacctacaa
     1621 ggtccacctc gacggctacg accttgcacc tgcgctcaaa ggcgacggcg actggccgcg
     1681 caaggaattc atctactgga cggatgacgg cagtgttgcc gccttacgct acgacgactg
     1741 gaaggtcacc ttcctggagc agacggcgga aggctatcgc gtttggcagg accccttcac
     1801 cgcgctgcgc gcgccgaccc tgaccaatct gcgcatggac cccttcgagc gtgccaggca
     1861 ggaagatgcg atgggctatc agcgctggtg gatcgagcac atgtatgtgt tcgcgcccgc
     1921 cgccgcctat gtggggcaat cgctgcaaag cttccgcgat tttccgccgc gccagaagcc
     1981 cggcagcttc aatctcgacc gggtgatgga ggcggtgacg gccccttccc cggtcaacta
     2041 gcgatgccgg cctttagccg ctgaaaccgc gaggcttggc gaacagcacc cggacacccc
     2101 gggtgctgtt ttcgtttggt cagcctccgg ccgagcgcaa aagcctggac agccggcccg
     2161 gagggggtgg tcagggaagc cgaattctgg tttttctgat ctttgattcc cggtcttctg
     2221 gcttccggtc tcacccccga tgttccaggt ctatctgccc attgccgaaa tctcggtgaa
     2281 ccttctggtg atgctggggc tcggcgcggc ggtgggcttt ctgtccggca tgttcggcgt
     2341 gggtggcggc ttccttttga ccccgctcct gatgttctcc gggatctcgg ccccgatcgc
     2401 ggtcgcgacc ggcgccaatc aggtcgtcgc cacctcggta tccggcgcgc tcgcgcaatg
     2461 gcggcgcggc aatatcgact tccatatggg cgcggtcctg atcgcgggcg gcgtggtggg
     2521 ggcgctcgcg ggcgtgttcc tgctcaaggt cttgcgcgag gcggggcaag ccggcctcat
     2581 cgtctctctg acctatgtgg tgctgctcgg cgcggtgggc acgctgatgc tgggcgagag
     2641 cctccgcgca ctggggcgcg cccgtgccgg cacgcccctc cccgcgcggc gctcggggca
     2701 gcacaattgg attcaccggc tcccgctcaa gctccgcttt ccccgctcgc gcctctacat
     2761 cagcgccatc cctccgctcg tgatcggcgc cgtcgtcggc ctgctca
//
LOCUS       DASUQP010000028        23767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_12826,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000028 DASUQP010000000
VERSION     DASUQP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 23767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23767
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12826"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFY74_01300"
     CDS             complement(<1..181)
                     /locus_tag="VFY74_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930375.1"
                     /translation="MVRKSLIAGLGFLGVLLAGTAGGPAPAAAGYACGPWNNWCVPRC
                     GSWNNWCRPVCGPWNG"
     gene            324..731
                     /locus_tag="VFY74_01305"
     CDS             324..731
                     /locus_tag="VFY74_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5930376.1"
                     /translation="MSDWSKLEAPGLAAFEAIAGEAYARLPARFRALTGDILIRVEDF
                     PTDEVLDSLNIESPFDLLGLYSGIDLARKSVSDPPSVPDMVFLYRRPILDYWAEHEET
                     LGAVITHVLVHEIGHHFGLSDADMERIEAEAVA"
     gene            826..1329
                     /locus_tag="VFY74_01310"
     CDS             826..1329
                     /locus_tag="VFY74_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930377.1"
                     /translation="MQSAARLMMGLALLPLLAATSPIDRDAAALEQGRLVAPGRLVVA
                     GRKLSCGKIATLLRDFEGFAVSSTVIMLNMQALKDLPRPVQWLIYYHECGHIVYGPSE
                     SAADCYAVRRARREGWLSGAALDEICAVFNIAGHGPSHPDPEKRCEQLRQCFTNKRGV
                     TARDEAR"
     gene            1371..2006
                     /locus_tag="VFY74_01315"
     CDS             1371..2006
                     /locus_tag="VFY74_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930378.1"
                     /translation="MRLTMLDGRAFLRMALSCAAIVALCAAYSGAGHAQQWAGKEGST
                     TSGPSLDEYLKQIGPDGSVVGHGQLKIDGKAVNCGKRPTVLNPNFDSWGGAFPGFLIL
                     NTKKIDGLSTQVKLYVYSHECGHQFEGADETKADLFAIRRGVKWGWLDAQGLEDICVF
                     IAQLKGDAVHPPGPQRCETMRSYYRKLIEAAGQQAKVPASTAKPQTLGPPE"
     gene            complement(2022..2342)
                     /locus_tag="VFY74_01320"
     CDS             complement(2022..2342)
                     /locus_tag="VFY74_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930379.1"
                     /translation="MAGPSDSRERNEALRLFVAATIGVLLLVIIGGAGLYYLAGLGDA
                     DPFSAGLGLKNAALIAFGVSFAVILVMAVVSGGDAIFGELPFTILGFLIFFVVFWLMI
                     AWIF"
     gene            complement(2366..2764)
                     /locus_tag="VFY74_01325"
     CDS             complement(2366..2764)
                     /locus_tag="VFY74_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5930380.1"
                     /translation="MTDTVIYEAHPAMFRAHPFWFILSVLLIAAFGIGIVILVYWYIL
                     TRATALTVTDSDILYERGILSKDRTSVSLRHVRSVNVMQSFVNRIFGVGTIQISTAGD
                     EPEFTIADMPDPHVIREAIAKAQEMREDRN"
     gene            2882..3487
                     /gene="leuD"
                     /locus_tag="VFY74_01330"
     CDS             2882..3487
                     /gene="leuD"
                     /locus_tag="VFY74_01330"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5930381.1"
                     /translation="MQKFTTLTGIAAVLPMINVDTDMIIPKQYLKTIKRTGLGKGLFA
                     DIRYDEKGAPLPDFVLHKPPYNEAKILITGENFGCGSSREHAPWALLDFGIRCLIAPS
                     FADIFYNNCFKNGILPITLPQAEIDKLIDDAGRGANATLTIDLPAQEIRGPDGGKIHF
                     EIDPFRKRCLMEGLDDIGLTLEKAEAIDAFERRARLERPWA"
     gene            3953..5536
                     /locus_tag="VFY74_01335"
     CDS             3953..5536
                     /locus_tag="VFY74_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017263677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5930382.1"
                     /translation="MTGSSLAQKRARERLIAGHCQGGSPEMASMRSRQVLPGKPESCW
                     TATAPKTSYPKLSGSQKAEVVVVGAGIVGVTAAYLLREAGVSVALVEARRIGRQVTGR
                     STAKITTQHSLIYRYLIDIFGLQTARHYADANRLGMNQIRQWVEQLGIACAFAAKDAY
                     VYCNDSSRLGELEAEAEASRQAGLDSEVLEAAPLPFATCGALRSRDQAQFNPAQYLIG
                     FAKAAEAAGVLVFEETRVTSVEEKDGWHLKAGRASIHGESVVLATAMPIAGPVRYDKR
                     SRPRSHICMAFRVDAGAAIDGMFIGVDEPTHSLRMGRDGDGLLLVVLGAKFVTGLEGD
                     IAKHFRELEAWTRQNLEVGDVAWRWVNEDYDTPDRLPLVGASADAPGLYIATGFNAWG
                     ISNGTAAGILIAQQILGNSPPWASIYDPERKLSEKFNKGGNSQSVVRSLDDIASGEGG
                     VMNLGQGKIAVWKGDDGVPHAVSASCTHEGCIVTWNNADRTWDCPCHGSIFTADGSVI
                     HGPAVEPLPPRKLPPNWLR"
     gene            complement(5832..6248)
                     /locus_tag="VFY74_01340"
     CDS             complement(5832..6248)
                     /locus_tag="VFY74_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930383.1"
                     /translation="MLTRAVFALVVILAAPAAALAETKVLALGLSDHEVSQEELETGA
                     ALPVPRFNTGAIAYVSAAELKKGDVVEIALKKDGKPLHRNSETLAEDRARFLLQAGKR
                     GVPAGGWPEGNYQAAAKISRDGKTLIEQTSAPIPIE"
     gene            6363..7469
                     /gene="leuB"
                     /locus_tag="VFY74_01345"
     CDS             6363..7469
                     /gene="leuB"
                     /locus_tag="VFY74_01345"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5930384.1"
                     /translation="MTTIKLLLLPGDGIGPEVMGEVERVVAWVNRTGLASIETETDLV
                     GGSAYDAHGQAVSNATMDRALAADAVMLGAVGGPKWDGVPYDQRPEAGLLRLRKDLAL
                     FANLRPAICYPALADASSLKRDRVDGLDIMIVRELTGGVYFGEPKTISDLGNGQKRAI
                     DTQVYDTHEIERIATVAFDLARERKRLVHSAEKRNVMKTGVLWNEVVTKLHKERYGDV
                     TLHHILADNCAMQLVRAPKQFDVIVTDNLFGDMLSDEAAMLTGSLGMLPSASLGEADE
                     REHRRALYEPVHGSAPDIAGQGIANPIATIASLGMCLRYSFFLGDTADRIDRAIARVL
                     DQGLRTADIKQEGCRTVGTREMGEAIVAALDGAG"
     gene            complement(7486..8259)
                     /locus_tag="VFY74_01350"
     CDS             complement(7486..8259)
                     /locus_tag="VFY74_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930385.1"
                     /translation="MHWWSLLALRRPIACLGAASCLLVLSAHIAAAQFYVRSPDVKKG
                     VTALEEHGAFYSGPGDDERRRQSHEVELKHGLTDRWELILEGTFRQDIGESFEARRLE
                     LGAQYEIVERRGDGWGVAFRTLYEFALQDDIPDEILFGPLAKYVRGKDSFTINTFFVG
                     QVGSEAEIDSLELKVNWRLKHEFNDRFGLGVEGYSEIEDLANAGSFNDQEHRLGPVAY
                     WEFEGRGGRTEWEFAAGTLFGISDAVSDVTYKFDIEFKF"
     gene            8471..9169
                     /locus_tag="VFY74_01355"
     CDS             8471..9169
                     /locus_tag="VFY74_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930386.1"
                     /translation="MNPALRLLAVAALLFASGALALAAEGDAAQGAAGQAPAAAEPAE
                     AKRVLTPEEEAEKEARKACKTKICDIIATRDPNGEDVTCDIVKTWREEDIVKTLGGSI
                     GWPWGKAVCQSKLELQRKPLALAMSEPAHEIVMPVQKVRCTLAQKTEGEPFTVEVSLA
                     PKVKFENGKATEAKINWGEASAPLLIYPLIYAGTGLDNETNVLGPEVVRMVNEFTTKK
                     CTELKVELPGPSPN"
     gene            9267..9962
                     /locus_tag="VFY74_01360"
     CDS             9267..9962
                     /locus_tag="VFY74_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX5930387.1"
                     /translation="MDNLNHIEAQTATPPEPSPSGELPRPSAANSRRTSALLRDFIAA
                     ETGSRVSLGALRDALGDRGFGVLLFIFALPNLVPVNIPLLSVVLGFPLVLLGAQLAYG
                     RHKPWFPAWLTRQTFSRQGFAAVVTRALPYLERAERLLRPRLTFLLSWTGERLIGIAI
                     LILALVLFAPIPFANWPPACAIAIMGLAIVEKDGVAVLVGLAVGVVSLMVAATVVVGL
                     IKAFLLLFSGMFG"
     gene            complement(10062..11387)
                     /locus_tag="VFY74_01365"
     CDS             complement(10062..11387)
                     /locus_tag="VFY74_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX5930388.1"
                     /translation="MIALLRATLLVLLAALLAGCDSAALTDAETQGPNPKIPPPSEGP
                     LPAVNIAPAVGWPEGATPKPAEGLAVNAFATGLDHPRWFHILPNGDVLVAETNAPKKE
                     GPGGFKAWVMAKAMSLAGAGVPSPNRIILLRDGDGDGVAETRTVFLEGLNSPFGMALV
                     GNKLYVADTDALLSFDYQEGAAELKTQGTKIGDLPAGQINHHWTKSLAANRDGSRLYV
                     GVGSNSNVAENGLANEEGRAQILEIDPLTGQSRSFATGLRNPVGLAFEPQSGVLWVVV
                     NERDELGADLVPDYLTSVKEGAFYGWPFSYWGQHVDERAKPQNPELVAKAIAPDYSLG
                     NHVAPIGLAFAQGTSLPEAFRSGAFIGEHGSWNRIPYSGYKVVFVPFAEGTPSGAPVD
                     VLTGFLSDQGEAQGRPAGVAIDRTGALLVADDVGNIVWRVTQAARSVMR"
     gene            11503..12528
                     /locus_tag="VFY74_01370"
     CDS             11503..12528
                     /locus_tag="VFY74_01370"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5930389.1"
                     /translation="MGYKVAVIGATGNVGREMLNILAEREFPADEVFAIASRRSMGVE
                     VSFGDQRLKCRDLEQFDFRGVDFALMSAGSAISKEWSPRVGAQGCVVIDNSSCWRYDP
                     DVPLIVPEVNADAVAGFTKKNIVANPNCSTAQLVVVLKPLHDRAKIKRVVVATYQSVS
                     GAGKEAMDELWDQTKGIFATAAPAPKKFTKQIAFNVIPHIDVFLDDGSTKEEWKMAAE
                     TKKILDPKIKLIATCVRVPVFVGHSEAVNVEFEEPLTAEEARTILREAPGVLVIDKRE
                     PGGYVTPVECVGDYATFVSRIREDQTLDNGLSFWVVSDNLRKGAALNTVQIAETIINR
                     NLLDRAA"
     gene            complement(12547..13179)
                     /locus_tag="VFY74_01375"
     CDS             complement(12547..13179)
                     /locus_tag="VFY74_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5930390.1"
                     /translation="MQLPRTLVEGHFRFRRDEHTRNRGRYLQLAELGQTPTAMVIACC
                     DARVDVSAIFDAEPGALFIMRNVANLVPPYEPEGKYHGTSAAVEYAAMALKVPHIIVL
                     GHSHCGGVNAYRRGVRENVRGQGFIGSWLTLLDNLKLVESDVFTYGDAMAFELAAIRS
                     SLANLGSFPFVKKRVEDGLLALHGLHFDIGSGQLLALAPEKERFTALIEA"
     gene            13388..14593
                     /locus_tag="VFY74_01380"
     CDS             13388..14593
                     /locus_tag="VFY74_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEX5930391.1"
                     /translation="MAKVLVVLYDDPVTGYPPKYARDDIPKIDHYPDGMTTPTPSKVD
                     FKPGELLGSVSGELGLRKFLEDAGHTLVVTSDKDGPNSVFEKELSDAEIVISQPFWPA
                     YMTKERFDKAKKLKLVVTAGIGSDHTDLQAAMDKGITVAEVTYCNSISVSEHIVMMIL
                     ALVRNYIPSYGWVIKGGWNIADCVERSYDLEGMNIGTVAAGRIGLAALRRLKPFDVKL
                     HYTDKHRLPEKVEKELDLTFHPNVESLVKVCDVVTINAPLHPETEGMFNDKLIGKMKR
                     GAYLVNTARGKIVDRDAVVRALESGQLAGYAGDVWFPQPAPRDHPWRTMPHHGMTPHI
                     SGSSLSAQARYAAGVREILESYFAKKPIRTEYLIVDKGKLAGTGAHSYTAGDATKGSE
                     EAAKFRKGA"
     gene            14753..16690
                     /locus_tag="VFY74_01385"
     CDS             14753..16690
                     /locus_tag="VFY74_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020810958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX5930392.1"
                     /translation="MASAGGSIAASDRIGLLDKARIIAPPDYNRWLVPPAALAIHLCI
                     GMAYGFSVFWLPLGRAVGAAQGLDKAIPCPGDATTFFGKVAITAQALFATDCDWTQFD
                     LGWMYTLFFVLLGVSAAIWGGWLERVGPRKAGVVSACCWCGGLMISAIGVYLHQLWMM
                     WLGSGFIGGIGLGLGYISPVSTLIKWFPDRRGMATGLAIMGFGGGAMIGSPLATILMN
                     YYDTPTSVGVWETFVTLGVGYFIFMMSGAFGYRLPPPGWKPEGWTPPASGKAMIATGH
                     VHLRDAHKTVQFWLLWAVLCLNVSAGIGIIGAASPMLQETFGGRLFGDASVGFADFTD
                     DQKAKAALVGAAFVGLFSLFNIAGRIFWASLSDTIGRKATYFIFFSLGFACYAASPTL
                     ATLGALALFAAAFCLIASMYGGGFATIPAYLADIFGTQFVGAIHGRLLTAWSTAGIVG
                     PVIVNYMHDARKAAGVPPDQIYHPIFYVLAGLLVGGFVANLLIRPLNKKWFMKPEEVE
                     ALQVKTAVTSARRDTETRPAAAVNRNIRLLQPAGMALIAVALVWWIHFYTKAGGIDGA
                     WECLVFTPSFCQSAIAQAELAGSFAYRPFLMWIGLALWLAGIVLNPRRIEIAPFAWAA
                     VGIPLLWAVWITIEKALVIFT"
     gene            16926..17015
                     /gene="pqqA"
                     /locus_tag="VFY74_01390"
     CDS             16926..17015
                     /gene="pqqA"
                     /locus_tag="VFY74_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HEX5930393.1"
                     /translation="MKEWLKPEITESEAGMEVTSYLPAELDRA"
     gene            17231..18307
                     /locus_tag="VFY74_01395"
     CDS             17231..18307
                     /locus_tag="VFY74_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5930394.1"
                     /translation="MNEKQSREIDRTVTGNFFEDFKLGQEIVHATPRTVTVGDVSLFT
                     ALYGPRFAVQSSDAFAQGIGYPRSPIEDLLVFHIVIGKTVPDISRNAIANLGYAEFRF
                     LAPVYPGDTLASVSEVIGVKENSNRKSGTVYVRSTGKNQRGQKVLSYARWVMVNKRNP
                     DSPSPSPVVPELASSVDPATLGTALPPLTLSGYDDALAGSPRRFDDYAVGDRIDHVDG
                     QTIEEAEHQLATRLFQNTARVHFNQHIEGKGRFGRRLVYGGHIISIARSLSFNGLANA
                     FHILGLNGARHVAPSFAGDTIYAWSEILDKAELPGRSDMGALRLRTFAVKDRSCADFP
                     GLKADGNHEDGVVLDMDYWVALPR"
     gene            18442..18960
                     /locus_tag="VFY74_01400"
     CDS             18442..18960
                     /locus_tag="VFY74_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX5930395.1"
                     /translation="MRQKLVSAAIVLGAALALGSPAGAADSASASLKDASGNQVAKAT
                     LTNAKEGVLIRLDLSAVPAGEHAFHIHAVGKCEPPDFKSAGPHFNPDTSKHGLMNPEG
                     PHAGDMPNLHVPADGKLSIEVLNPTVTLSADSALLDEDGAALVIHAGSDDYKSDPAGN
                     AGDRIACGVITP"
     gene            19054..19461
                     /gene="sdhC"
                     /locus_tag="VFY74_01405"
     CDS             19054..19461
                     /gene="sdhC"
                     /locus_tag="VFY74_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX5930396.1"
                     /translation="MGKARLGAERPLSPHLQIYRPMLTMMMSIAHRISGTANAAGFVL
                     LAMWLVAIAAGPQAYAQVSSVFASIPGRIVLFLFTWSLVHHMLGGIRHLIWDTGRGLD
                     RTSIEVFAWFTILGSTALTILIWYAGYALRGSQ"
     gene            19463..19837
                     /gene="sdhD"
                     /locus_tag="VFY74_01410"
     CDS             19463..19837
                     /gene="sdhD"
                     /locus_tag="VFY74_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX5930397.1"
                     /translation="MATPLKRVRGLGAAHGGTETFWRQRMTAIANIPLVLFLIYAIVS
                     HIGADYGTVRAYFAQIPVALLMLALVISAAIHMRIGLKEIIEDYVHGEGAKVAAILLA
                     TFFAAGVGLAAVLAILKIAFGS"
     gene            19843..21690
                     /gene="sdhA"
                     /locus_tag="VFY74_01415"
     CDS             19843..21690
                     /gene="sdhA"
                     /locus_tag="VFY74_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX5930398.1"
                     /translation="MAQDATLQDAPPHNAVQINGRAYPIVDHAYDVIVVGAGGAGLRA
                     VVGCSEAGLRTACITKVFPTRSHAVAAQGGIAAALGNMGPDDWRWHMYDTVKGSDWLG
                     DQDAIEYLCRNAPEAVYELEHWGVPFSRTEDGKILQRAFGGMTTDFGKGQAKRTCAAA
                     DRTGHALLHTMYGQALRHSAEFFIEYFAIDLIMDDEGRCRGVVALSMQDGTLHRFRAH
                     QVILATGGYGRAYFSCTSAHICTGDGNAMVLRAGLPLQDMEFVQFHPTGVYGAGVLIT
                     EGARGEGGFLRNSEGEKFMERYAPSAKDLASRDVVSRAITIEIREGRGHGPDQDYVHL
                     HLDHLDDALIEERLPGITESARIFAGVDVREAPIPVTPTCHYNMGGIPTNMHGEAISP
                     RDGDPDAIVPGLMALGEAACVSVHGANRLGSNSLIDLVVFGRAAAHRCAELITKGGTI
                     PDLPNGAGEQAIARLDRFRHANGATPTAALRLRMQKLMQRDCAVFRTKNVLQEGANAI
                     RAVWDDTADIRVTDRSLIWNTDLIETLEFDNLIAQSAVTVEGALAREESRGAHAREDF
                     PKRDDQNWMKHTLSWADDRSHKVKLDYRPVHKFTLSNEVSYIEPKERVY"
     gene            21705..22493
                     /locus_tag="VFY74_01420"
     CDS             21705..22493
                     /locus_tag="VFY74_01420"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX5930399.1"
                     /translation="MVQFTLPRNSKVRKGKAWNPPPDGAPAARYRVYRIYRFDPETGE
                     NPRLDTYWVDKEECGPMVLDALIWIKNNVDPTLTFRRSCREGVCGSCSMNIAGTNTLA
                     CTMHADDAKGAIAIYPLPHMPVIKDLVPDMTGFFAQLRAIEPWLKTDSPTPQTEWRQS
                     KEDRDKLDGLYECILCACCSTACPSYWWNSDRYLGPAILLQADRWIEDSRDERTGERL
                     DELEDPFRLYRCHTILNCTKACPKGLNPAAAIASLKRKLVERAG"
     gene            22522..22953
                     /locus_tag="VFY74_01425"
     CDS             22522..22953
                     /locus_tag="VFY74_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5930400.1"
                     /translation="MIDHMGMPVGDIARATEFYRKALAPLGYGLVMEVSAAETGHGAA
                     IGFGPPGKAADFQSGKPSFWIGEGERSSGPVHVAFLAPSRAAVDAFYRAALDAGGKDN
                     GPPGLRPHYHADYYAAFVFDPDGNNIEAVCHRPRDTIFPSP"
     gene            complement(23141..>23767)
                     /locus_tag="VFY74_01430"
     CDS             complement(23141..>23767)
                     /locus_tag="VFY74_01430"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX5930401.1"
                     /translation="NIRTYWRGDDGKPYRTFAALADDLEAKGRSLRFAMNGGMYRNDL
                     RPVGLYIENGRELTAANTATLTGAPSQIPNFYKKPNGVFYIGDGEAGIVETSRFLALR
                     PRANFATQSGPMLVIDGAIHPAFIVRSSDRKPRNGVGVSSRTRAQFVITRGWVTFHEF
                     ARFFRDGLGCDNALFLDGGAASGLYAPELGRDDAPGHGGYGPIIAAVD"
BASE COUNT         4279 a         7805 c         7369 g         4314 t
ORIGIN      
        1 atccgttcca cggtccgcac accgggcggc accaattgtt ccaagacccg cagcgcggca
       61 cgcaccaatt gttccaaggt ccgcaggcat agcctgccgc ggccggggct ggtcctcccg
      121 ccgtgccggc cagcagcacg ccaaggaacc ctaaaccggc gatcaaactc tttctaacca
      181 tgtcgttacc ctttctgctc acccggtccg caggagcgcg ggctcgggtc ttggcggcga
      241 aactggcacg agtggcctga accgggtctg atcgctacat tcacgtgccg ttcagttttc
      301 gaggcttggc agcggtggcg aagatgagcg attggagcaa gcttgaagcg ccgggtctcg
      361 ccgcgttcga ggccatcgca ggagaagcct atgcgcggct gcctgcccgc ttccgcgcgc
      421 tcaccggcga tatcctgatc cgcgtcgagg actttccgac cgacgaggtg ctcgattcgt
      481 tgaacatcga atcgccgttc gatctgctcg gcctctattc cgggatcgat cttgcgcgaa
      541 agagcgtgag cgacccgccc tcggtgccgg acatggtgtt cctctaccgg cgcccgattc
      601 tcgattactg ggccgagcac gaggagacgc tcggcgccgt gatcacccat gttctggtgc
      661 atgagatcgg ccatcatttc ggcctgtccg atgccgacat ggagcgcatc gaggccgaag
      721 ccgtcgctta agggcgccgc ttcagcgatt tggcgttgac gcttgcgctc ccccctcgca
      781 gactgcgggc cggcaagggg aaccgaggca gcagagggaa cgacaatgca atcagctgcc
      841 cggctcatga tggggcttgc gctcctcccg ctcctcgccg cgacgagccc gatcgatcgc
      901 gacgccgccg cgctcgaaca aggccggctc gttgcgccgg gccgtctcgt ggtcgccggg
      961 cgaaagctct cttgcggcaa gatcgcgacg ctccttcgcg actttgaagg cttcgccgtt
     1021 tcctccaccg tcatcatgct gaacatgcag gcgctcaagg atctgccgcg gcccgtgcag
     1081 tggctgatct actaccacga atgcgggcat atcgtttatg ggccgagcga atcggcggcc
     1141 gactgttacg cggtgagacg cgccaggcgc gagggctggc tgagcggcgc ggcactcgac
     1201 gagatttgcg cggtgttcaa tatcgccggt cacggtccct cccatcccga tccggagaag
     1261 cgctgcgagc agctcaggca atgcttcacc aacaagcgcg gcgtcaccgc acgtgacgag
     1321 gcgcgctgat tgttttgcgg cgattgcgcg tgctgcttgg gagaattaga gtgcgactca
     1381 ccatgctgga cggacgggcg tttttgcgga tggcgctttc ctgcgctgca atcgtcgcgc
     1441 tttgcgcggc ctattctggc gcgggccatg cccagcaatg ggcgggcaag gagggctcca
     1501 ccacctccgg ccccagcctc gacgaatatc tgaagcagat cgggcccgac ggcagtgtcg
     1561 tcggccatgg tcaactcaag atcgacggca aggccgtcaa ttgcggcaag cggccgacgg
     1621 tgctgaaccc caatttcgac agttggggcg gcgcctttcc cggcttcctg atcctcaaca
     1681 ccaagaagat cgatgggctc tcgacccagg tgaaactcta cgtctactcg catgagtgcg
     1741 ggcatcagtt cgaaggcgcc gacgagacca aggcggacct cttcgccatc aggcgcggcg
     1801 tcaaatgggg ctggctcgac gcccaggggt tggaggacat ttgcgtcttc atcgcgcagc
     1861 tgaaaggcga cgcggtccac ccgcccggac cgcagcgctg cgagaccatg cgcagttatt
     1921 accgcaagct gatcgaggcc gccggccagc aagctaaagt gcctgcctcg acggcgaagc
     1981 cgcaaacgct ggggccgccc gagtagcgcg cgtgggtcgc ctcagaagat ccaggcgatc
     2041 atcagccaga acaccacgaa gaaaatcaaa aagccgagaa ttgtgaacgg cagctcgccg
     2101 aagatggcgt cgccgcccga gaccaccgcc atgaccagaa tgacggcgaa ggagacgccg
     2161 aaggcgatca gcgccgcgtt cttgagtccg aggcccgccg agaacgggtc ggcatcgcca
     2221 agaccggcaa ggtaatagag accggcgccg ccgatgatga cgagcagcaa aacgcctatc
     2281 gtcgcggcga cgaacagacg aagcgcctcg ttgcgttcgc gactgtcgct cggtcctgcc
     2341 atcgccgggt tctcctccgc atccctcagt tgcggtcctc gcgcatctcc tgcgccttgg
     2401 cgatcgcctc ccggatcaca tgcggatcgg gcatgtcggc gatggtgaac tcgggctcat
     2461 cgcccgcggt cgagatctga atcgtgccga ccccgaaaat gcggttgacg aagctttgca
     2521 tgacgttcac ggagcggacg tgccggagcg acaccgaggt gcggtctttg ctcaagatcc
     2581 cgcgttcgta caagatatcg ctgtcggtga cggtgagcgc ggtggcgcgc gtcaggatgt
     2641 accaatagac cagaatgaca atgccgatgc cgaaggcggc gatcagcaag accgaaagga
     2701 tgaaccagaa gggatgcgcc cggaacatgg ccgggtgggc ttcgtagatc acggtgtctg
     2761 tcattttcgc tcccacatcc cgcgcttatg acccgcggac cttagtcgcg gcgcgcccat
     2821 ctgaaaagct ggaaagcgca gccgcttgag gctacataac cccggatcgc aagaggcact
     2881 catgcagaaa ttcaccacgc tcacggggat cgccgcggtt ctgccgatga ttaatgtcga
     2941 caccgacatg atcattccga agcaatatct caagaccatc aagcgcaccg ggctcggcaa
     3001 gggcctgttc gccgatatcc gctatgacga aaagggcgcg cccttgcccg atttcgtgct
     3061 gcacaagccg ccctacaatg aagccaagat cctcatcacc ggcgagaatt tcggctgcgg
     3121 ctcctcccgc gagcacgcgc cgtgggcgct gctcgatttc ggcattcgct gcctgatcgc
     3181 gccgtccttc gccgacatct tctacaacaa ctgcttcaag aacggcattc tccccatcac
     3241 gctcccgcaa gcagagatcg acaagctgat cgacgacgcc ggccgcggcg ccaatgccac
     3301 gctcaccatc gaccttcccg ctcaggagat caggggaccg gacggcggca agatccactt
     3361 cgagatcgac ccgttccgca agcgctgcct gatggaaggt ctcgacgata tcgggctcac
     3421 cctcgagaag gccgaggcca tcgacgcgtt cgaacggagg gcccgcctcg aacgcccctg
     3481 ggcctgagta cccgcattgc ttggccgttg ttgcggtcac ggtaacattc ccctctcccc
     3541 ttgagggaga gggcttctcg cccggcgcgc gaagcgcgac gagggtgagg ggtaaatctt
     3601 ttgacgccgg cttcgcccgc ttacccctca ccgcgcgcca ccacgcgctc aagcagcgaa
     3661 gcggtagcgc atcgaacacg ccgcgcgtgc tctccccaac ctgcctgcgc gaagccgaag
     3721 cggcttcgct tcggcgaagg caggggggag agcgaaagat gttgcacagc ccagaccgtc
     3781 atcctgaggt gcgagcatgc attttaaacc tccccctcgt ggggaggtcg gcgcgctctc
     3841 gcgcgccggg tgggggctct ctcggatcac tcctacacgt cattccggcg aaagccggaa
     3901 tccagtagcg gcatgtactg cgcttgtttt ctggataccg gcttacgccg gcatgacggg
     3961 atcgagtttg gcacaaaaac gggcgcgaga gcgtttgata gcaggacact gtcagggagg
     4021 cagtcctgaa atggcgtcaa tgcgatcccg gcaggtccta ccgggtaagc cggagtcttg
     4081 ctggaccgcg acggcaccga agacctcata tccgaagctc tcaggctcgc agaaagcaga
     4141 ggtggtcgtc gtcggcgcgg gcatcgtggg ggttactgcc gcctacctgc ttcgtgaggc
     4201 cggcgtatcg gtggcgctcg tggaagcgcg tcgcatcgga cggcaagtca cagggcgttc
     4261 cacggcgaag atcaccacgc agcattccct catctatcga tatctcatcg acatcttcgg
     4321 tcttcaaacg gcgcggcact atgccgacgc aaatcgcctc ggcatgaacc aaatacggca
     4381 gtgggtcgag cagcttggta tcgcctgcgc ttttgcggcc aaagacgcct atgtctattg
     4441 caacgattca tcgcgtctcg gggagctcga ggctgaggca gaagcttcgc gtcaagcggg
     4501 gctcgattca gaggtgctcg aagccgcgcc ccttcctttc gcaacctgcg gcgccttgcg
     4561 atcgcgagac caggcgcaat tcaatccggc gcaatacctg atcggctttg cgaaagcggc
     4621 tgaagcggcc ggcgtccttg tcttcgaaga gacgcgcgtg acttctgtcg aggagaagga
     4681 cggctggcac ctcaaggccg gccgagcctc aatccacggc gagagtgtgg tgttggccac
     4741 cgccatgccg atcgcaggtc ccgtgcgcta cgacaagcgc agccggccgc ggtcgcacat
     4801 ttgcatggct tttcgcgtcg atgcaggcgc ggcgatcgac ggcatgttca tcggcgtcga
     4861 cgaacccacg cattctctca gaatgggccg cgacggcgac ggtctgttgc tggtggttct
     4921 gggcgccaaa ttcgttacgg gcctggaagg cgacatcgcg aagcattttc gcgaactcga
     4981 agcctggaca aggcaaaatc ttgaagttgg cgatgtcgca tggcggtggg tcaatgaaga
     5041 ttacgatacc cccgatcggc tgccgctcgt cggagcatcg gctgacgcgc cggggctcta
     5101 catcgccact ggcttcaacg cctggggcat cagcaacggc acggcggcgg gcatcctgat
     5161 tgcccaacaa attctcggca actcaccccc atgggcatcg atttacgatc ctgagcgcaa
     5221 gctctcggaa aagttcaaca agggtggcaa tagccaatcg gtcgtgcgta gcctcgacga
     5281 tatcgcgtca ggcgaaggtg gcgtgatgaa tcttgggcag ggcaagattg cggtatggaa
     5341 aggggacgac ggcgtccctc atgccgtctc ggcgtcttgc acgcacgaag gttgcatcgt
     5401 gacgtggaac aatgccgacc gaacctggga ctgtccctgc cacggctcca tcttcacggc
     5461 agatggcagc gtcattcatg gaccggcggt cgagcccttg ccgccgcgga agctgccgcc
     5521 aaactggctt cgctaatctt tgccatccgc gcgtcatcct gaggtgcgac ctgcctgcgc
     5581 gaagccgggg cttcgctccg gctaaggcag ggcggagcgg ccacctcagg atgacgacgc
     5641 ttcaaaaagt agctcgtcac gccgttacct cgcagcgaga tgaagcgcca atggaatgga
     5701 ttgccggagc ctgttcccgg acttgatccg gcggatcaag cccggcaatg acggtgacct
     5761 tgggtgtcac tcaatcgcgg gcgcgcaggc gcggcctgga gcatgaccct tacccacccg
     5821 actcccaaac gctactcgat tggaattggc gccgacgtct gctcgatcag cgtcttgccg
     5881 tcgcgcgaga tcttggcagc ggcctgatag ttgccttccg gccagccgcc ggcgggaacg
     5941 ccccgcttgc ccgcttgcag cagaaaccgc gcccggtcct cggccagcgt ctcactgttg
     6001 cggtgcaagg gctttccgtc tttcttgagc gcgatctcca ccacatcgcc cttcttcagc
     6061 tcggccgccg agacataggc gattgccccc gtgttgaaac gggggacggg gagcgccgcg
     6121 cccgtctcaa gctcttcctg gctgacctcg tggtcggaaa gtccaagcgc gagcaccttg
     6181 gtctccgcca gggcagccgc cggcgctgcc agaatgacca ccaacgcaaa caccgctctt
     6241 gtcagcatcg ggctctcccg ttacccaaac cgcacgtctt cagtgggtct acatgaggca
     6301 aaaatcaatc ctcgtcatgg ccgggcacag gagtttatgc agccaaagga gcgacacggc
     6361 ctatgacgac cattaagctt ttgctgcttc ccggcgacgg catcggcccc gaagtgatgg
     6421 gcgaggtcga gcgcgtcgtc gcctgggtga accgaacggg gctcgcctcg atcgagacgg
     6481 agaccgatct ggtcggcggc tcggcctatg acgcccacgg ccaggccgtc tccaacgcca
     6541 ccatggaccg cgcgcttgcc gccgatgccg tcatgctcgg cgcggtgggc ggaccgaaat
     6601 gggatggcgt gccttacgac cagcgcccgg aagcgggcct gcttcggctc cgcaaggacc
     6661 ttgcgctctt cgccaatctt cgtcccgcca tctgttatcc cgcgctcgcc gacgcctcct
     6721 cactcaagcg cgaccgcgtc gacggcctcg acatcatgat cgtgcgcgag ttgaccggcg
     6781 gggtctattt cggcgaaccg aagaccatca gcgatctcgg caacggccag aagcgcgcca
     6841 tcgatactca agtgtacgac acccacgaaa tcgagcgcat cgccaccgtc gccttcgatc
     6901 tcgcccgcga gcggaagcgg ctggtgcact cggccgagaa acgcaacgtg atgaagaccg
     6961 gcgtgctgtg gaacgaggtg gtcaccaaac tccacaagga gcgctatggc gacgtgacgc
     7021 tgcaccatat ccttgccgac aactgcgcca tgcagctcgt gcgcgcgccg aagcagttcg
     7081 acgtgattgt caccgacaat ctgttcggcg acatgctgtc tgacgaggcc gccatgctta
     7141 caggctcgct cggcatgctg ccctcggctt cgctcggcga ggccgacgag cgcgagcatc
     7201 gtcgcgcgct ctacgagccg gtgcacggct cggcgcccga catcgcaggt cagggcatcg
     7261 ccaacccgat cgccaccatt gccagcctcg gcatgtgcct gcgctattcc ttcttcctcg
     7321 gcgacaccgc cgaccgcatc gaccgggcga tcgcgcgcgt gctcgaccag ggcctccgca
     7381 cggccgatat caagcaggag ggctgccgca ccgttggcac gcgcgagatg ggcgaggcga
     7441 tcgtcgccgc gctcgacggc gccggctagc gccgcgacaa agaccctaga atttgaactc
     7501 gatgtcgaac ttgtaggtga cgtcggagac agcgtcggag atcccgaaca gggtgccggc
     7561 cgcgaattcc cactcggtcc tccccccgcg cccctcgaac tcccaatagg cgactgggcc
     7621 taggcggtgc tcctgatcgt tgaacgagcc cgcgttggcg agatcctcga tctcgctgta
     7681 accctccacg cccagcccga atctgtcgtt gaattcgtgc ttcaggcgcc aattgacctt
     7741 gagctcgaga ctgtcgatct cggcctcgct gccgacttgc ccgacgaaga acgtgttgat
     7801 cgtgaaagag tccttgcccc tgacatattt cgcgagcggc ccgaacagaa tctcgtcggg
     7861 gatgtcgtcc tggagcgcga actcgtaaag ggtgcggaac gcaacgcccc acccgtcgcc
     7921 gcgccgctcg acgatttcat actgagcgcc gagttccagt cgcctggctt cgaagctttc
     7981 gccgatatcc tgccggaacg tcccctcgag gatcagctcc caccggtcgg tcagcccgtg
     8041 cttgagctcg acttcgtgcg attgcctgcg ccgctcgtcg tctcccggac cggaatagaa
     8101 cgcgccgtgc tcctcaaggg cggtcacgcc ctttttcaca tcgggcgagc gcacgtagaa
     8161 ctgggcggcg gctatgtgcg ccgagagcac aagcaggcat gatgcggcgc ccaggcaagc
     8221 gatcggccgc cggagcgcaa gaaggctcca ccaatgcacg gtatccccct aaccccgtcg
     8281 caacggccaa gcctagggcg ctccgccaat tgaccgaaat gaaaagttta catttgaaac
     8341 catgaacaaa attcatcagt aggcgatata tgcgggagcg ttctgcctac cagatgtcag
     8401 gtatcaggta tcaggtgtca gatgtcggcg agtctgatac ctgatacctg ttacctggtc
     8461 ggcaagaaga atgaatcccg ctttgcgcct gttggccgtc gccgccttgc ttttcgcttc
     8521 aggcgcgctc gcgcttgccg cggaaggcga tgcggcgcaa ggcgccgccg ggcaggcgcc
     8581 ggccgcggca gagcctgccg aggccaaacg ggtgctgacg ccggaggagg aggccgagaa
     8641 ggaggcgcgc aaggcctgca agaccaagat ctgcgacatc atcgccaccc gtgacccgaa
     8701 cggcgaggac gtaacgtgcg acatcgtcaa gacctggcgc gaggaggaca tcgtcaagac
     8761 gcttggcggc agcatcggct ggccctgggg caaggctgtg tgccagtcga agctcgagct
     8821 tcagcgcaag ccactcgctt tggcgatgag cgagccggcc cacgaaatcg tcatgccggt
     8881 gcagaaagtg cgctgcacgc tcgcgcagaa aaccgagggc gagcctttca cggtcgaggt
     8941 gagcctcgcg cccaaggtga agttcgagaa cgggaaggcg acagaggcaa aaatcaattg
     9001 gggcgaggcc tcggcgccgc ttctcatcta tcccctcatc tatgccggca ccgggctcga
     9061 caatgagacc aacgtgctcg gacccgaggt cgtacgcatg gtcaacgagt tcacgaccaa
     9121 gaaatgtacc gaactgaagg ttgaactgcc gggcccgagc ccgaactaga gtggagccgg
     9181 tcccgcgctt tcagttcgtt cctgtcaggt gaacgaaacg tcacagtcgg ggcttaatct
     9241 gcccctggca atcataccgt aacgcgatgg acaatctgaa ccacatcgag gcgcagaccg
     9301 ccaccccgcc cgagccaagc ccctcaggcg agctgcctcg cccgtcggcg gcgaacagcc
     9361 gccgcacgtc cgccctcttg cgcgatttca tcgccgccga gacgggctcg cgcgtcagtc
     9421 tcggcgcgct ccgcgatgcg ctcggcgatc gcggctttgg cgtccttctt ttcattttcg
     9481 ccctgccgaa tctcgtgccg gtgaacattc cgctgctttc agtcgtcctg ggctttccgc
     9541 tcgtgctgtt gggggcgcag cttgcctatg ggcgccataa gccttggttc cccgcttggc
     9601 tcacgcgcca gaccttctcg cgccaaggct tcgccgccgt cgtcacgcgc gcgcttccct
     9661 atctggaacg cgccgagcgc ttgcttcgcc cgcggctcac gtttctcttg tcctggaccg
     9721 gcgagcgcct gatcggcatt gccatcctca ttctcgcctt ggtcctcttt gcgccgatcc
     9781 ccttcgctaa ctggcccccg gcctgcgcca tcgccatcat gggtcttgcc atcgtcgaga
     9841 aggatggcgt tgccgtcttg gtcgggctcg ccgtgggtgt ggtgagtctg atggtggcgg
     9901 cgacggtggt ggtgggcctg atcaaggcct tccttctgct cttttccggc atgtttggct
     9961 gaggcacggc ttcttccccg tccttcccgg gtcaggtctg gccgctggcc ctggataacc
    10021 ggcgtggatc gtttccccga tggccagaaa ggcgatcttc gctagcgcat gacgctccgc
    10081 gccgcttgcg tcacgcgcca tacgatattg ccgacatcgt cggcgaccag aagcgcgccg
    10141 gtgcgatcga tggcgacgcc ggccggacgg ccctgggcct cgccttgatc gctgaggaat
    10201 cccgtcagca cgtcgaccgg cgcgcccgaa ggcgtccctt cggcaaaggg gacgaacacc
    10261 accttgtagc cgctgtacgg aatgcggttc cacgatccat gctcgccgat gaacgccccg
    10321 ctcctgaacg cttccggcaa cgacgtgcct tgcgcgaagg cgagtccgat cggtgccacg
    10381 tgattgccaa gcgaatagtc gggcgcgatc gcttttgcca ccagctccgg gttctgcggc
    10441 ttcgcgcgtt catcgacatg ctggccccaa tagctgaacg gccagccgta gaaggcgccc
    10501 tccttcaccg acgtgagata gtcgggcacg agatcggcac cgagctcgtc gcgctcgttc
    10561 accaccaccc acagcacgcc gctttgcggc tcgaacgcaa ggccgaccgg attgcgcaat
    10621 cccgtggcga agctgcggga ctgcccggtc aggggatcga tctcgaggat ttgcgcacgg
    10681 ccctcctcgt tggcgagacc attctccgcg acgttgctgt tagagccgac accgacatag
    10741 agccgcgacc cgtcgcggtt cgcggcaagg ctcttggtcc agtgatggtt gatctgaccg
    10801 gcggggagat cgccaatctt ggtaccctgc gtcttcagct cggccgcgcc ttcttggtag
    10861 tcgaagctga gcagcgcgtc ggtatcggcg acatagagct tgttcccgac gagcgccatg
    10921 ccgaagggcg agttgagacc ttcgagaaag acggttctcg tctcggccac gccgtcgccg
    10981 tcgccgtcac gcaagagaat gatgcgattg gggctcggca cgccggcgcc tgcgaggctc
    11041 atggccttgg ccatcaccca tgccttgaac ccgcccgggc cctccttctt gggcgcgttg
    11101 gtctcggcca cgagaacgtc gccgttcggc aagatatgaa accagcgggg gtgatcgagg
    11161 cccgtggcga aggcgttgac ggcaagaccc tcggcaggtt ttggcgttgc gccctcaggc
    11221 cagccgacgg ccggcgcgat atttacggcg ggaagcggcc cctcgctcgg cgggggaatt
    11281 ttcggattgg ggccttgcgt ctccgcatcg gtcagcgcgg ccgaatcgca tccggcgagg
    11341 agagcggcaa gcaacacgag aagggtggcg cggagcaggg caatcatggt cggtccgggg
    11401 ccagagtctt tctcgcgcac aacgctttac aaccgcgggt caatccccgt aaatcggccg
    11461 aaaattgccg ccaacgcgct tgaacgaagg agcatcggaa gcatgggtta caaagtcgcg
    11521 gtcatcggcg cgaccggaaa tgtcggtcgc gagatgctca acatcctagc cgagcgcgag
    11581 ttcccggccg atgaggtgtt cgccatcgcc tcgcggcggt cgatgggggt cgaggtctcg
    11641 ttcggcgatc agcggctgaa atgccgggat ctcgagcagt tcgactttcg cggcgtcgat
    11701 ttcgcgctga tgtcggcggg ctccgccatt tccaaggagt ggtccccgag ggtcggcgcc
    11761 cagggctgcg tcgtgatcga caactcgtcg tgctggcgct acgaccccga cgttccgctc
    11821 atcgtgcccg aggtcaacgc cgacgcggtc gccggcttca ccaagaagaa catcgtcgcc
    11881 aatccgaatt gctcgactgc ccagcttgtg gtggtgctta agcccctgca tgaccgcgcc
    11941 aagatcaagc gcgtcgtcgt tgcgacctat cagtcggtgt cgggcgccgg caaggaggcg
    12001 atggacgagc tgtgggacca gaccaagggc atcttcgcga ccgccgctcc ggcgccgaag
    12061 aaattcacca agcagatcgc cttcaacgtc attccccata tcgacgtctt tcttgatgac
    12121 ggctcgacca aggaggaatg gaagatggcg gccgagacca agaagatcct cgacccgaag
    12181 atcaagctga tcgcgacctg cgtgcgcgtg cccgtgttcg tcggccattc cgaggccgtc
    12241 aatgtcgagt tcgaggagcc gctcacggcc gaggaggccc gcacgatctt gcgcgaggcg
    12301 ccgggcgtgc tcgtgatcga caagcgcgag cccggcggct acgtgactcc ggtcgaatgc
    12361 gtgggcgact atgcgacttt tgtcagccgc atccgcgagg atcagacgct cgacaacggc
    12421 ctgtccttct gggtcgtgtc cgacaatttg cgcaaaggcg ccgcgctgaa cacggtgcag
    12481 atcgccgaaa cgatcatcaa ccgcaacctg ctcgaccgcg ccgcttaagc ccggcggcgg
    12541 ggcagcctac gcctcgatca gggccgtgaa gcgttccttt tccggcgcaa gcgcgagcaa
    12601 ttggccgcta ccgatatcga agtggaggcc gtgtagcgcc agcagcccgt cctcgacgcg
    12661 cttcttgacg aacgggaagc tgccgagatt cgccagcgac gagcgaattg cggcaagctc
    12721 gaaggccatc gcgtcgccat aggtgaagac gtcgctttcg acgagtttca gattgtcgag
    12781 cagcgtgagc cagctgccga tgaagccttg ccctcgaacg ttctcgcgca cgccgcgacg
    12841 ataagcgttg acgccgccac aatgcgaatg cccgagcacg atgatgtggg gcaccttgag
    12901 cgccatggcg gcatattcca ccgcggcgct cgtaccgtga tatttgcctt ccggctcgta
    12961 aggcggcacg agattggcga cgttgcgcat gatgaagagc gcgcccggct ctgcatcgaa
    13021 gatcgctgag acgtcgacgc gcgcatcgca acaggcgatc accatcgccg tcggcgtctg
    13081 gccaagctcg gcgagctgga gatagcgtcc gcgattcctg gtatgctcgt cgcggcggaa
    13141 gcggaaatgc ccctcgacca gcgttcttgg cagttgcaag gcctcgcctc cccaaaagcc
    13201 gaactctggg cagcatttca gccgcgtccg cttttgtcca gatcggccga tagagcgcgc
    13261 ctatcggccg ttcgggagcg cccacttgat tcacggcgtt gacgggagca aggctgcggc
    13321 gatcggcaac gcagaggcgc ggcctcaggc gcggcctcag ccgcgcccgc cgaaggagag
    13381 tgtccccatg gcaaaggtcc tcgttgttct ctatgacgat cccgtcaccg gctatccgcc
    13441 gaaatatgcc cgcgacgata tccccaagat cgaccactat cccgacggga tgacgacgcc
    13501 cacgccgagc aaggtcgact tcaagcccgg cgaactgctg ggcagcgttt cgggcgagct
    13561 cgggcttcgc aagtttctcg aggacgccgg acacacactg gtggtgacct cagacaagga
    13621 tggtcccaac tccgtcttcg agaaggagct ttcagatgcc gagatcgtca tctcccagcc
    13681 cttctggccc gcctacatga ccaaggagcg cttcgacaag gcgaagaaac tcaagctcgt
    13741 ggtcaccgcc ggcatcggct cagaccacac cgatctgcag gcggccatgg acaaaggcat
    13801 caccgtcgcc gaagtcacct attgcaactc gatcagcgtc tccgagcata tcgtgatgat
    13861 gatcctcgcg ctcgtccgca actacatccc gtcttacggg tgggtaatca aaggaggctg
    13921 gaacatcgcc gactgcgtcg agcgctccta cgatctcgaa ggcatgaata tcggcactgt
    13981 cgccgccggc cgcatcggac ttgccgcgct ccgtcgcctg aagcctttcg acgtcaagct
    14041 gcactacacc gacaagcatc ggctgccgga gaaggtggag aaggaactcg atctcacctt
    14101 ccatcccaat gtcgagtcgc tggtcaaggt ctgcgacgtg gtcaccatca acgcgccgct
    14161 tcaccccgag accgaaggta tgttcaatga caagctgatc ggcaagatga agcgcggcgc
    14221 ttatctcgtg aacacggccc gcggcaagat cgtcgatcgc gacgccgtcg tgcgcgcgct
    14281 cgaaagcggg cagcttgcag gctatgccgg cgacgtgtgg ttcccgcagc ctgcgccgcg
    14341 cgatcatccc tggcgcacta tgccccatca cggcatgacc ccgcatatct ctgggagctc
    14401 gctctcggcc caggcgcgtt atgccgcggg cgtgcgcgag atacttgaga gctatttcgc
    14461 caagaagccg atccgcaccg agtatctgat cgtcgataag ggcaagcttg ccgggaccgg
    14521 cgcccattcc tatacggccg gcgatgccac caaggggtcc gaggaagcgg cgaaattcag
    14581 aaaaggcgct tagcgggaca ccggcggtca ggcttgcgcc gcggatttcg ctcaagcaac
    14641 ggctcatgtg ccgcgggcat agttgcgcgg tgacgtgccg cgcttggccc ttgcggtcac
    14701 gggcaagccc ggccagactg cggcccctga cgcgcaggtt ggggcaggac ctatggcaag
    14761 tgctggggga tctatagccg cgagcgaccg tatcggcctg ctcgacaagg cgaggatcat
    14821 tgcgccgcca gactataacc gctggctggt gccgcctgcc gcgctcgcca tccatctctg
    14881 catcggcatg gcctacggct tcagcgtgtt ctggctgccg ctcgggcgcg ccgtcggcgc
    14941 cgctcaaggt ctcgacaagg ccatcccgtg tcccggcgac gcgaccacct tcttcggcaa
    15001 ggtggccata acggcgcagg ccttgttcgc caccgactgc gattggaccc agttcgatct
    15061 cggctggatg tacacactgt tcttcgtcct gctcggcgtg tcggcggcaa tctggggcgg
    15121 ctggctcgag cgggtcggac cgcgcaaagc cggcgtcgtc tcggcgtgtt gctggtgcgg
    15181 cggcctgatg atctcggcga tcggcgtcta ccttcatcaa ctttggatga tgtggctcgg
    15241 ctccgggttc atcggcggca tcgggctcgg tctcggttac atctcgccgg tctccacgct
    15301 gatcaagtgg tttcccgatc gccgcggcat ggccacgggc ctcgccatca tggggttcgg
    15361 cggcggggcc atgatcggct cgccgctcgc caccatcctg atgaactact acgacacgcc
    15421 cacttcggtc ggcgtgtggg agacgttcgt caccctcggc gttggctact tcattttcat
    15481 gatgagcggc gccttcggct atcggctgcc gcccccgggt tggaaacccg aaggctggac
    15541 gcctccggca agcggcaagg cgatgatcgc gaccgggcat gtccacctca gggacgcgca
    15601 caagacggtg cagttctggc tgctctgggc ggtgctgtgc ctcaacgtct ccgccggcat
    15661 cgggatcatc ggcgccgcct cgcccatgct gcaagagacg ttcggcggca ggcttttcgg
    15721 cgacgcttcc gtcggcttcg ccgatttcac cgacgaccag aaggccaagg cggcgctggt
    15781 cggtgccgcc ttcgtcggcc tcttctcgct gttcaacatc gccggccgca tcttttgggc
    15841 ctcgctctcc gacacgatcg gccgcaaggc cacgtatttc atcttctttt cgctcggctt
    15901 tgcctgctat gccgcgtcgc cgacccttgc cacgctcggc gcgcttgcct tgtttgccgc
    15961 cgccttctgc ctgatcgcct cgatgtacgg cggcgggttc gctactattc cggcctatct
    16021 cgccgacatc ttcggcacgc aattcgtggg cgccattcac ggacgcctgc tgaccgcctg
    16081 gtcgaccgcc ggcatcgtcg ggccggtgat cgtcaactac atgcatgatg cgcgcaaagc
    16141 ggcgggcgtg ccgccggacc agatctatca cccgatcttc tacgtcctgg cgggcctgct
    16201 ggtgggaggt tttgtcgcca atcttttgat ccggccgctc aacaagaagt ggttcatgaa
    16261 gcccgaggag gtcgaagccc tacaggtcaa gacagccgtc acatccgcgc gacgggacac
    16321 cgagactcgt ccagcggcgg cggtcaatcg gaacattcgc ctgctccagc cggctggcat
    16381 ggcgctgatc gcggtcgctc tcgtctggtg gatccatttc tataccaagg caggaggcat
    16441 cgacggcgcg tgggagtgcc tcgtcttcac cccgagcttc tgccagagcg caatcgctca
    16501 ggccgagctt gccggcagtt tcgcctatcg ccccttcctg atgtggatcg ggctcgcgct
    16561 gtggctcgcc gggatcgtgc tgaacccaag gcggatcgag atcgccccct tcgcctgggc
    16621 ggcggtcggc attccgctgc tgtgggcggt gtggatcacg atcgaaaagg cgctggtcat
    16681 tttcacttaa gcggccggac cggccggcac tgttttgcaa ttcggctttg tcccgggccg
    16741 tggctggctt cagcctcaat atccgggatc ctgaggacca ggaattcccc gaaatcgagc
    16801 ctagcgggca aattcctgtc agaatttctt aatgtggctt tcagggagcg ttcatggtcg
    16861 cgtcatgcag ccctcatccc cagcccccat tatctaagct gttagttcac cgaaggagac
    16921 gaccaatgaa agagtggctc aagcccgaga tcaccgagtc cgaggccggc atggaagtga
    16981 ccagctatct tcccgccgag ctcgaccgcg cttaaccaag cgtcgatctt acaagttttc
    17041 gtctgaagat ggcggttccc acgaaagtgg ggatcgccat tttcgtgcct ggactggcct
    17101 ctttgctgcc cctcggctct tcacgcgccg atcatcggat tcggcggccc ggctcttccc
    17161 cgcgcctgtc cttgtctcgg cgtaggccgc aaactaaggt cggcgagcgc gaaggacgcg
    17221 atgagccgac atgaacgaga agcagagccg agagatcgac aggaccgtca ccggcaattt
    17281 cttcgaggat ttcaagctcg gacaggagat cgtgcatgcg accccgcgca cggtgacggt
    17341 gggcgatgtg agcctgttca ccgcgctcta cggtccgcgc tttgccgtgc agtcctccga
    17401 cgcattcgcg caaggcatcg gctatccgcg ttcgcccatc gaagatctcc tcgtcttcca
    17461 catcgtcatc ggcaagacgg tgccggacat ttcgcgcaac gccatcgcca atctcggcta
    17521 cgccgagttt cgcttcctcg cgccggtcta tcccggcgac acgctcgcct cggtctcgga
    17581 agtgatcggg gtcaaggaga actccaacag gaagagcggc acggtctatg tgcgctcgac
    17641 gggcaagaac cagcgcggac agaaagtgct ctcctatgcg cgctgggtga tggtgaacaa
    17701 gcgcaacccg gattcccctt ctccctcacc cgtggtgccc gagctcgcct cgagcgtcga
    17761 tccggcgacg ctcggcacgg cgttgccgcc gctcacgctt tccggctacg acgatgctct
    17821 ggccggctcg ccccggcgct tcgacgatta cgcggtcggt gacaggatcg accatgtcga
    17881 cgggcagacc atcgaagaag ccgagcacca acttgccacg cgcctcttcc agaacaccgc
    17941 gcgcgtccat ttcaaccagc atatcgaagg caagggacgg ttcggcagga ggcttgtcta
    18001 cggcggtcac atcatctcca tcgcccgctc cttgagcttc aacggccttg ccaatgcctt
    18061 tcacatcctc gggctgaacg gcgcgcgtca cgtggcaccc tctttcgccg gcgataccat
    18121 ctacgcctgg agcgagatcc tcgacaaagc cgagcttccc gggcgctcgg acatgggcgc
    18181 gctgcgcttg cgcaccttcg ccgtcaagga tcgctcttgc gccgactttc ccggcctgaa
    18241 ggcagacggg aaccatgagg acggcgtggt tcttgatatg gattattggg tggcgctgcc
    18301 gcggtagtct tgctttcgcc gtccagcggt tgtcatgccc gcgaaagcgg gcatccagta
    18361 tcccctgacc tcgaaccaaa tcgcgctgtc tgtagttact ggatcgtctg ctttcgcgga
    18421 cgatgacaca ggaggaaggc gatgcgacaa aaattggtct cggcggcgat tgttctgggg
    18481 gcagcgcttg ctctcggatc gccggcagga gcggccgaca gcgcgagtgc gagcctgaag
    18541 gacgcgagcg gcaatcaggt cgccaaggcc acgctgacga atgcgaaaga gggtgtcctg
    18601 atccgcctcg atttgagcgc cgtgcctgcg ggcgagcacg ccttccatat tcatgccgtg
    18661 gggaagtgcg aacccccgga cttcaaatcg gcgggccccc atttcaaccc tgacacatcc
    18721 aagcacgggc tgatgaaccc tgaaggcccc cacgccggcg acatgccgaa cctgcatgtc
    18781 cccgccgacg gcaagctcag catcgaggtg ttgaatccca ccgtgacctt gagcgccgac
    18841 tccgcgctgc tcgacgagga cggcgcggct ctcgtcatcc atgcggggtc cgacgactat
    18901 aagagcgacc cggccggaaa tgccggcgac cgcatcgcct gcggcgttat cacgccctag
    18961 ctcggcatta agcccaagcc gggttcgatt gcttgagccc tccgacttgc gttatgagag
    19021 aggctcgtct gggcaagcaa ccgcacgaga accatgggca aggcgaggct aggggcggag
    19081 cggccgctct ccccgcatct gcagatttac cggccgatgc tgacgatgat gatgtcgatc
    19141 gcgcatcgca tctcgggcac ggcgaatgcg gcgggctttg tcctgctcgc catgtggctg
    19201 gtcgccatcg ccgccgggcc gcaagcttac gcccaagtct cgagcgtctt cgccagcatc
    19261 cccgggcgca tcgtgctgtt tctgttcacc tggtcgctcg tccatcacat gctgggcgga
    19321 atccgtcacc tgatctggga taccggtcgc ggcctcgaca ggacgagcat cgaggtgttc
    19381 gcctggttca ccatactcgg ctcgacagcg ctcacgattc ttatctggta cgcgggctac
    19441 gcgctgaggg ggagccaata gcatggccac gccgctgaaa cgggtgcgcg ggcttggcgc
    19501 cgctcacggc ggcaccgaga ccttctggcg ccagcgcatg acggcgatcg ccaacatccc
    19561 gctcgtgctg ttcctgatct acgcgatcgt cagccatatc ggcgccgatt acggcacggt
    19621 gagagcctat ttcgcgcaaa tccccgtggc tctcctcatg ctcgcgctcg tcatctccgc
    19681 cgccatccat atgcgcatcg ggctgaagga gatcatcgag gattatgttc acggcgaagg
    19741 cgcgaaggtc gccgcgatcc tgcttgcgac tttcttcgcg gccggcgtgg gtctcgccgc
    19801 cgttctcgcc atcctcaaga tcgctttcgg atcctaggcg ccatggctca agacgcaacg
    19861 ttacaggacg ccccgccgca caacgcggtc cagatcaatg gccgcgccta tccgatcgtc
    19921 gaccatgcgt atgatgtgat cgtagtcggc gccggaggcg caggtctgcg ggccgtagtc
    19981 ggctgcagcg aagcgggctt gcgcaccgcc tgcatcacca aggtgttccc cacccgctcg
    20041 cacgccgtcg cggcgcaagg cggcatcgcg gctgcgctcg gcaatatggg tcccgacgac
    20101 tggcgctggc acatgtacga cacggtcaag ggctccgatt ggctcggcga ccaggacgcc
    20161 atcgaatatc tctgccgcaa cgcgcctgag gccgtctacg agctcgaaca ttggggcgtg
    20221 cccttctcgc gcaccgagga cggcaagatc ctccagcgcg ccttcggcgg catgaccacg
    20281 gatttcggca agggccaggc caagcgcacc tgcgccgccg ccgaccgcac cggtcacgcc
    20341 ctgttgcaca cgatgtacgg ccaggcgctg cgtcattcgg ccgagttctt catcgaatat
    20401 ttcgccattg atctcatcat ggacgacgag ggacgctgcc gcggcgtcgt cgcgctgtcg
    20461 atgcaggacg gaaccttgca tcgtttccgc gcccaccagg tcattctcgc caccggcggc
    20521 tatggccgcg cctatttctc ctgcacctcc gcccatatct gcaccggcga cggcaacgcc
    20581 atggtgctcc gcgcaggtct cccgctccag gacatggagt tcgtgcagtt ccatccgacc
    20641 ggcgtctatg gcgccggtgt gctcatcacc gagggcgcgc gcggcgaagg cggtttcttg
    20701 cgcaattccg agggcgagaa attcatggag cgctatgcgc cctcggccaa agacctcgcc
    20761 tcgcgcgacg tcgtctcgcg cgccatcacc atcgagatca gggaggggcg cggccacggt
    20821 cccgaccagg actacgtcca tctccatctc gaccatctcg acgatgcgct gatcgaggag
    20881 cggctccccg gcatcaccga gtcggcccgc atctttgccg gcgtcgacgt cagagaggcg
    20941 ccgatccccg tcacgcccac ctgccactac aatatgggtg ggatcccgac caacatgcat
    21001 ggcgaggcga tcagcccgcg cgacggcgac cccgacgcca tcgttccggg gctgatggcg
    21061 cttggcgagg cggcttgcgt gtccgtgcat ggcgccaatc ggctcgggtc caattccctg
    21121 atcgatctcg tcgtgttcgg ccgcgccgca gcgcaccgct gcgccgagct catcaccaag
    21181 gggggaacca ttcccgatct gcccaacggc gcgggcgagc aggcgatcgc gcggctcgat
    21241 cgattccgcc atgccaatgg cgcaacgccc acggccgcgc ttcgcttgcg catgcagaag
    21301 ctcatgcagc gcgactgtgc ggtgttccgc accaagaacg tgctgcaaga gggcgccaac
    21361 gccattcgcg ccgtttggga cgacaccgcc gacatccgcg tcaccgaccg ctcgctgatc
    21421 tggaataccg acctgatcga gacgctcgaa ttcgacaacc tcatcgctca atccgcggtc
    21481 acggtcgaag gcgcgcttgc ccgtgaggag agccgcggcg cgcatgcgcg cgaggacttc
    21541 cccaagcgcg acgaccagaa ctggatgaag cacacgctgt cttgggccga tgaccggagc
    21601 cacaaggtca agctcgacta tcgcccggtg cacaaattca cgctgtcgaa cgaagtctcc
    21661 tatatcgagc ccaaggagcg ggtgtactag ccttgaggtc gacgatggtt caattcacgc
    21721 ttcccaggaa ctcgaaggtt cggaaaggca aggcctggaa ccctccgccc gacggcgccc
    21781 cggccgcgcg ctatcgcgtg taccgcatct accgcttcga tcccgagacc ggggagaacc
    21841 cgcgtctcga tacctattgg gtcgacaagg aggagtgcgg gccgatggtg ctcgacgcgc
    21901 tcatctggat caagaacaac gtcgacccca cgctcacctt ccgccgctca tgccgcgaag
    21961 gcgtgtgcgg ctcctgctcg atgaacatcg ccggcaccaa cacgctcgcc tgcaccatgc
    22021 atgccgatga cgccaaaggc gcgattgcca tctatcccct gccgcatatg ccggtgatca
    22081 aggacctcgt gcccgacatg acgggcttct tcgcccagct ccgcgccatc gagccttggc
    22141 tcaagacgga tagcccgacg ccgcagacgg agtggcgcca gtcgaaggaa gatcgcgaca
    22201 agctcgacgg gctttacgag tgcatcctct gcgcctgctg ctcgaccgcc tgcccctctt
    22261 attggtggaa cagcgaccgc tatctcggcc cggcgatcct cttgcaagcc gatcgctgga
    22321 tcgaggacag ccgcgacgag cggaccggcg aacgcctcga cgagctcgag gacccgttcc
    22381 ggctctatcg ctgccacacg atcctcaact gcaccaaggc ctgccccaag gggctgaacc
    22441 cggcagcggc catcgcctcg ctcaagcgca agcttgtcga gcgcgcgggc tagggggcgc
    22501 gcctagacaa ggacaggcgc catgatcgac catatgggca tgccggtcgg cgacatcgcc
    22561 cgcgccaccg agttctaccg caaggccttg gcgccgttag gctatggcct ggtgatggag
    22621 gtctcggcgg ccgagaccgg gcacggcgcc gcgatcggct tcggccctcc cggcaaggcg
    22681 gcggacttcc agagcggcaa gccttcgttc tggatcggcg agggcgagcg ctcgagcgga
    22741 ccggtccatg tcgctttcct cgcgccgtca cgcgcggccg tcgatgcttt ctatcgcgcg
    22801 gcgcttgacg cgggcggaaa ggacaacggc ccgccggggc ttcgcccgca ttaccacgcc
    22861 gattactatg ccgccttcgt gttcgacccc gacggcaaca acatcgaggc ggtgtgccat
    22921 cgcccgcgtg acaccatttt tccctcccca tgatcgggag ggtggcgagg atgagcagag
    22981 ccactcgtgg cggagcgaaa tccgaagccg ggtggggcga gacagtcccc accccagcgt
    23041 cgcctcgcgc tcaagttgcg aagcggtagc gcgacaacag atgggcgacg ccggacctcc
    23101 ccgcaggggg aggtatggtt ttgccaacag cgaacaagtg tcaatcgact gcggcgatga
    23161 tcgggccgta gccgccgtgg ccaggcgcgt cgtcgcggcc gagctcgggc gcatagagtc
    23221 cggatgccgc gcctccgtcg agaaagagcg cgttgtcgca gccgagtccg tcgcgaaaaa
    23281 aacgggcaaa ctcgtgaaag gtcacccatc cccgggtgat aacgaactgc gcccgtgtcc
    23341 ggctcgacac gccgactccg ttccgcggct tgcgatcgct tgagcgaacg atgaaggccg
    23401 ggtggatcgc cccgtcgatg acgagcatcg gaccggactg cgtggcgaag tttgcgcgtg
    23461 gcctcagggc gaggaaacgg ctcgtctcga caatgcctgc ctcgccgtca ccgatgtaga
    23521 aaacgccatt cggcttcttg tagaaattcg ggatctggct gggggcgccg gtgagggtcg
    23581 ccgtattggc cgccgtcagc tcgcggccat tctcgatata gagtccgacc ggccgaagat
    23641 cgttccggta catgccgccg ttcatggcaa agcgcagcga cctgcccttg gcttcgaggt
    23701 cgtcggccag cgctgcaaag gtccgatagg gcttgccgtc gtctccgcgc caataggtgc
    23761 ggatatt
//
LOCUS       DASUQP010000029         5056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1239899, whole genome shotgun sequence.
ACCESSION   DASUQP010000029 DASUQP010000000
VERSION     DASUQP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239899"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..945
                     /locus_tag="VFY74_01435"
     CDS             106..945
                     /locus_tag="VFY74_01435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HEX5930402.1"
                     /translation="MWRALSLLFTVALAGAGFYLLTPEGRQLIDRVRINWTRAEYQAR
                     FRAGETLPGTPDLARFEQRLAAHGVKLGLPVYIRVFKLESELELWVEKGGRFERFATY
                     PICLWSGRLGPKLKEGDRQAPEGFYTVDSEQLNPNSKMHRSFSLGFPNVFDQAHGRTG
                     SFLMVHGGCASIGCYAVTDPAVDEIWRMVTAALEQGQPRFPVHVFPFRMTDRALDFRK
                     GYRWHGFWADLKKGYDLFQPSRIPPAVSVCNGRYVFEPGTIDTANGLVEERCAPKIAD
                     KPA"
     gene            1221..1580
                     /locus_tag="VFY74_01440"
     CDS             1221..1580
                     /locus_tag="VFY74_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5930403.1"
                     /translation="MSDVQRYSDEVQRAGKDGFDAAVSSFGEANKGFQAIAAEITAYS
                     KKSFEDGTRAFEQLLGAKSFEQVIEIQAQYARMAYEGYVAELTKLGKMWAGLTRNAYQ
                     PVEQAAAKAGVAHRRSA"
     gene            complement(1953..4715)
                     /gene="secA"
                     /locus_tag="VFY74_01445"
     CDS             complement(1953..4715)
                     /gene="secA"
                     /locus_tag="VFY74_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5930404.1"
                     /translation="MLSFGALASKVFGSSNERKLKKFGPIVAKINALEAETEALSDEA
                     LRARTEQFRARVREGTELDDLIPEAFATVREAAKRTLGQRHFDVQLVGGMVLHEGCIA
                     EMKTGEGKTLVATLPVYLNALTGRGVHVVTVNDYLAKRDAAWMGAIYKFLGLTVGCIV
                     HELSDQERREQYACDVTYGTNNEFGFDYLRDNMKYASGEMVQRGHVFAIVDEVDSILI
                     DEARTPLIISGPLDDRSDLYNTIDTFIPKLEAPDFELDEKTRVVTLTEAGNEKVERML
                     SEAGLLKDDSLYDIENVTVVHHVQQALRAHKLFQRDRDYIVKNDEVIIIDEFTGRMMQ
                     GRRYSEGLHQALEAKEHVAIQPENQTFATITFQNYFRLYEKLAGMTGTAATEADEFME
                     TYGLDVAEVPTNMDMIRVDEDDEVYRTAQEKYRAIIKLIKEAQSRGQPMLVGTVSIEK
                     SEILSGLLKKENVPHQVLNARYHEQEAHIIAQAGVPGAVTIATNMAGRGTDIQLGGNP
                     DMLLEDWLTAEKEKGAEPQPDAVAKKRAEIASLVGTRKKEALDAGGLYVVGTERHESR
                     RIDNQLRGRSGRQGDPGRSRFFLSLEDDLMRIFGSQRMDGMLQALGLKEDEAIVHPWI
                     NKALEKAQGKVEARNFDIRKNLLKYDNVMNDQRKAIFEQRRELLGDADLNETVDEMRH
                     QVIDDLVATHIPANSYPEQWDIAGLTEAVKGIFDLDVPIADWAAEEGIGDAELHERLL
                     KATDEKTAKKAADIGPQIMRQIERAVLLQTLDNLWREHLVTLEHLRQVIGLRGYGQRD
                     PLNEYKSESFTLFEHMLARLREAVTGQLAHVELAKPDEVPPLEGAELPPMEAHHANPT
                     TGEDEFALETANAGGNGRGQAKPARARKAAEVNPGDPSSWGKVQRNALCPCGSGKKYK
                     HCHGAY"
BASE COUNT          900 a         1559 c         1580 g         1017 t
ORIGIN      
        1 gggtcaagcc cgggggtgac gaactctgga gcgcgtcaga attccgacga aacggaccta
       61 tactagaccc tcctgagttc tgacgtctga tacctggcac ctgagatgtg gcgagccctt
      121 tctctcctct tcaccgtcgc cctggccggc gcgggcttct atctgttgac gccggagggg
      181 cgtcagctga tcgaccgcgt gcgcatcaac tggacgcggg ccgagtacca ggcgcgcttc
      241 cgggccggag agaccttgcc cggcacgcca gacctcgccc gcttcgaaca gcggctcgcc
      301 gcccatggcg tcaagctcgg cctgcccgtc tacattcgcg tcttcaagct cgaatccgag
      361 cttgagcttt gggtcgagaa gggcggccgc ttcgagcgct tcgccaccta tcccatctgc
      421 ctctggtcgg gccggctcgg gccgaagctg aaagagggcg accggcaagc gccggagggc
      481 ttctacacgg tcgactccga gcagctcaat cccaacagca agatgcaccg ctccttcagt
      541 ctcggctttc ccaacgtgtt cgaccaggcg catgggcgca ccggatcgtt cctgatggtg
      601 catggcggct gcgcctcgat cggctgctac gcggtcaccg atcccgcggt cgacgagatc
      661 tggcgcatgg tgacggcggc gctcgaacag ggtcagccgc gcttccccgt tcatgtcttc
      721 cccttccgca tgacggaccg cgccttggat ttccgaaaag gctaccgctg gcacggcttc
      781 tgggccgatc tgaagaaggg ctacgaccta ttccagccga gccgcatccc gcccgccgtc
      841 agcgtgtgca acggccgtta cgtgttcgag ccgggaacga tcgacaccgc aaacggcctc
      901 gtcgaggaac gctgcgcgcc caagatcgca gacaagcccg catgatcgtc aaggcgccgc
      961 taggaagcgg acggcggctg caccgcccag atatttccgc agtttgaggg aatgcggtcc
     1021 aagcgtggaa atggctgttt gagcggcctt tgacctacag ctgtcaggat tcggcgtgtc
     1081 acgagggata aggacgcgcc gaggcacctt tgtgcaacgc aataattttc ggaaggctat
     1141 tgtgcagcgc accatagcat gccatatgaa tcctcacacg caacatgccc gcgccaggcg
     1201 gggctcagaa aggtcatcag atgtctgacg ttcaaaggta tagtgatgag gttcagagag
     1261 ctggcaagga cggctttgat gccgcggtca gctcctttgg tgaggcgaac aaggggttcc
     1321 aggctatcgc cgcggagatc accgcttatt cgaagaagtc gttcgaggac ggtacgcgtg
     1381 ctttcgaaca gctgctcggt gcgaagtctt tcgagcaggt tatcgagatc caggctcagt
     1441 acgccagaat ggcttatgaa ggctatgttg ccgagttgac gaagctcggt aagatgtggg
     1501 ccggtctgac gcgtaacgcc taccagccag tcgagcaagc cgcagccaag gctggggtgg
     1561 ctcatcgacg aagcgcctag ttcgacaaaa gacctgaggt cagcctcggg tctttcaatt
     1621 tcgcgcgacc gcgacgcggg agtacagagt tggtcgcgcg acacaagaga acgcagttga
     1681 agcaattgca cgaacataaa cgatccaggc atagattcat caccgggaac gtggtgcggc
     1741 tcagcaagcg ctatcccccg cgcaaggctg cgccgggtcc gtacaatgtc gttgcgcaac
     1801 ttcctgagcg tgacgggcaa ctccaatacc gcataaaaag cggctgcgag cccttccacc
     1861 gcaccgttgc agaaaatgag ctggaggctg aggtaggagc accggtcaca gtcgcggcaa
     1921 gccagccgtg aaagtgttta taagctcagg cgctagtagg cgccgtggca gtgcttgtat
     1981 ttcttgcccg agccgcacgg gcagagcgca ttgcgctgca ccttgcccca gcttgaagga
     2041 tcgccgggat tgacctcggc cgctttgcgc gcacgcgcgg gcttggcctg gcctctgcca
     2101 ttgccgccgg cattggctgt ctcgagcgcg aactcgtctt ccccggtggt cgggtttgca
     2161 tggtgcgctt ccatcggcgg aagctccgcc ccctccaatg gagggacctc gtccggcttt
     2221 gcgagctcca catgggcgag ctgtcccgtc accgcctcgc gcagccgcgc gagcatgtgc
     2281 tcgaacagcg tgaagctttc gctcttgtac tcattcaacg gatcgcgctg gccatagccg
     2341 cgaagaccga tcacctgacg cagatgttcg agcgtgacga ggtgctcgcg ccatagatta
     2401 tcgagcgtct gcaaaagcac cgcccgttcg atctggcgca tgatctgagg tccgatatcg
     2461 gccgccttct tggcggtctt ctcgtccgtg gccttgagca gccgctcgtg caactcggca
     2521 tcgccgattc cttcttctgc cgcccaatcg gcaatgggga cgtcgagatc gaagatgcct
     2581 ttcaccgctt cggtcagccc cgcgatgtcc cattgctcgg gataggaatt ggcggggata
     2641 tgcgtagcga ccagatcgtc gatgacctga tggcgcatct cgtcgacggt ttcgttgaga
     2701 tcggcgtcgc ctaacagctc gcggcgctgc tcgaagatcg ccttgcgctg atcgttcatg
     2761 acgttgtcgt atttgagcag gttcttgcgg atgtcgaagt tgcgcgcttc gaccttgcct
     2821 tgcgccttct ccagcgcctt gttgatccag ggatggacga tcgcctcgtc ctccttcaac
     2881 cccagcgcct ggagcatgcc gtccatgcgc tgcgagccga agatgcgcat cagatcgtcc
     2941 tcgagcgaca ggaagaagcg ggagcggccc gggtcgcctt gacggcccga acggccgcgc
     3001 agctggttgt cgatgcgacg gctctcgtgg cgctcagtgc cgacgacgta gagcccgccg
     3061 gcgtcgagcg cctccttctt tcgcgttccg acgagagagg cgatctccgc gcgcttcttg
     3121 gcgaccgcgt cgggctgcgg ctccgcgccc ttctccttct cggcggtaag ccagtcctcc
     3181 agcagcatgt cgggattgcc gccgagctga atgtcggtgc cgcgccccgc catgttggtg
     3241 gcgatggtga ccgcgccggg cacgccggct tgggcgatga tatgcgcttc ctgctcgtga
     3301 tagcgggcat tcagcacctg atgcggcacg ttctccttct tgagcaagcc ggagaggatc
     3361 tcggatttct cgatggaaac ggtgccgacc agcatgggtt ggccgcggct ctgcgcctcc
     3421 ttgatcagct tgatgatcgc ccggtacttt tcctgcgccg ttcggtaaac ctcgtcgtct
     3481 tcatcgacgc ggatcatgtc catgttggtc gggacttcgg ccacgtcgag gccataggtc
     3541 tccatgaact cgtcggcttc cgtcgcggcc gtaccggtca tgccggcaag tttttcatag
     3601 aggcggaaat agttctggaa ggtgatggtg gcgaaggtct ggttctcagg ctggatcgcc
     3661 acatgctcct tggcttcgag cgcctgatgc aggccttcgg agtaacgccg tccctgcatc
     3721 atgcggccgg tgaattcgtc gatgatgatg acctcgtcgt tcttgacgat gtagtcgcgg
     3781 tcgcgctgga acagcttatg agcgcggagc gcctgctgca catgatggac gaccgtgacg
     3841 ttctcgatgt cgtaaagcga gtcgtccttc agcagcccgg cttcgctcaa catccgctcg
     3901 accttctcgt tgcccgcctc ggtcaaggtg acgacgcgag tcttctcgtc gagctcgaaa
     3961 tcgggcgcct cgagcttcgg aatgaaggtg tcgatggtgt tgtagagatc ggagcggtcg
     4021 tcgagcgggc cggagataat gaggggggtt cgcgcctcgt cgatcaggat cgagtccacc
     4081 tcgtcgacga tggcgaagac gtgaccgcgc tgcaccatct cgcccgacgc atatttcata
     4141 ttgtcgcgaa gatagtcgaa gccgaactcg ttgttggtgc cataggtaac gtcgcaggca
     4201 tattgctcgc gccgctcttg atcgctgagc tcgtgcacga tgcagcccac ggtgagcccc
     4261 aggaacttgt agatggcgcc catccaggcg gcatcgcgct tggcgaggta atcgttgacg
     4321 gtgacgacgt ggacgccacg cccggtcagt gcgttgaggt agacggggag ggtcgcaacc
     4381 agcgtcttgc cctcgccggt cttcatctcg gcgatgcagc cctcatgcag aaccattccg
     4441 ccgacaagct gcacgtcgaa atgacgctgg ccgagcgtgc gcttggccgc ctcgcggacg
     4501 gttgcgaaag cctcggggat caaatcgtcg agctcggtgc cttcacgcac gcgcgcacgg
     4561 aactgctcgg tccgggcgcg caaggcctcg tcggagagcg cttcggtctc ggcctcgagc
     4621 gcattgatct tggccacgat cggaccgaat ttcttgagtt tccgctcgtt cgatgagccg
     4681 aagaccttgc tggcgagagc gccaaaagac agcatgagtc gttaagcctc tagagcgcgg
     4741 gcgatttccg cggccgatcc ccaccaaagc cttgccaaag ccgcacaaat gcggggcgcc
     4801 agcgttggtg agacataagt gggccgccta tccttgtcaa cgcggcgcgc cgacacccgc
     4861 cccctcaatg cggggaggga gactttgcca tgagatttca ccccacccgt cgcgcgaacg
     4921 cgcgaccgac ctcccgatcg aggggaggta tggcgcttga acgggctgga ccctaagctt
     4981 gctgtttcga cgggacttcc cggcgcatcc gcccgatcgc gtcgagctcc tgttgcagcg
     5041 agagcttact cagctg
//
LOCUS       DASUQP010000030        15178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_559994,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000030 DASUQP010000000
VERSION     DASUQP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15178
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559994"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..883
                     /locus_tag="VFY74_01450"
                     /pseudo
     CDS             1..883
                     /locus_tag="VFY74_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            998..1300
                     /locus_tag="VFY74_01455"
     CDS             998..1300
                     /locus_tag="VFY74_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930405.1"
                     /translation="MASDSDELEEQLRENLKLRRELGAEVAKAASRRAGMAYRLGRVL
                     YLACFGLAVLCVAVWIGRLFTDFAPMPILLQLLAFGLPALLFYGLGRAFRYVLSGE"
     gene            1603..2526
                     /locus_tag="VFY74_01460"
     CDS             1603..2526
                     /locus_tag="VFY74_01460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX5930406.1"
                     /translation="MFKPVQAGRPLKGRRRFAVGSFSRRFQPPKIYGRLGSLEVRLAR
                     KKSEIRRAQRLRYKVFYEEMSAKPNALAILSRRDEDVYDQIFDHLLVIDRANQSKGRW
                     RRSKVVGTYRVLRNEVADLYDGFYSQGEYDVAALIAAKSPDCSFMELGRSCVLKPYRS
                     RRALELLWQGIMAYVREHGVDVMIGCASFEGIDPNAHAEALSFLYHNALAPKEWRVRA
                     HEHLRIDMNMMPEAAVSHRAALKALPPLIKGYLRAGAYVGDGAVIDHQFGTIDVFIIL
                     PVAAIQRRYSEHFGARDTLASSLAPEFAPLN"
     gene            2619..3713
                     /gene="hrcA"
                     /locus_tag="VFY74_01465"
     CDS             2619..3713
                     /gene="hrcA"
                     /locus_tag="VFY74_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5930407.1"
                     /translation="MRHADPIETKSAAEAAASLRQLNERSREIFKRIVESYLETGEPV
                     GSRNLSRALPMTLSPASVRNVMSDLESLGLIYAPHTSAGRLPTQTGLRLFIDGLLEVG
                     DISVEERKEIESRIAGGGRRRAIEDLLGEAGEMLSGLSRCAGVVLVDKQSKRLKQVEF
                     VSLGERRALAVLVGEEGDVENRIVALPDGLPLSSLREASNYLNARIGGLTLAEARARI
                     EKEIEERRAQLDALTASIVTEGLATWSGDGDDRKSLIVCGRSHLLEDVRAMEDLERVR
                     LLFEDLDTKTDLMQLLGVAERAEGVRIFIGSENKLFSLSGSALIVSPFEDTEQKIVGV
                     LGVIGPTRLNYARIIPMVDYTAKLLGRLVP"
     gene            3781..4452
                     /gene="grpE"
                     /locus_tag="VFY74_01470"
     CDS             3781..4452
                     /gene="grpE"
                     /locus_tag="VFY74_01470"
                     /inference="COORDINATES: protein motif:HMM:NF010739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5930408.1"
                     /translation="MPNDKTDQGEADQDQATDPSKAQPANGDPEPDAQLGADLDALLA
                     ENAEMRDRLLRTMADMENLRRRTEREKADTARYAISNFARDVLTVGDNLKRTIDHVPQ
                     EAAAQDPALKSFLEGVELTERELLNVLERHGVTRIEPLGARFDPNCHQAMYEVQKADV
                     PEGTVVEVMQEGYVIGDRCLRPALVAVAKGGAKPTKPQAGKGLGATPPAAANDDFPDG
                     GWQGD"
     gene            complement(4522..5118)
                     /locus_tag="VFY74_01475"
     CDS             complement(4522..5118)
                     /locus_tag="VFY74_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX5930409.1"
                     /translation="MDFFPQALGREPQIALLAAIRGIIAEAPLFTPTMPRSGTPMSVR
                     MTNCGPLGWVTDKEGGYRYQATHPKTGRPWPPIPNMLLELWRDVAAYAAPPEACLVNY
                     YAGNAKMGLHQDKDEEDFAAPVLSVSLGDTGIFRAGGKSRRDPTRTFELKSGDVVVLG
                     GTDRLAYHGIDRILAGTSDLIPEGGRFNLTLRRVTKPK"
     gene            5196..6029
                     /locus_tag="VFY74_01480"
     CDS             5196..6029
                     /locus_tag="VFY74_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5930410.1"
                     /translation="MAARAQKQNPDGYVHAKDGTEYQRLRAQALLWQSATEAVLDRVG
                     LKAGMSCLDVGSGPGAVMRLMADRVGPAGRITGIEMDAALGAQALAELRAQGGSTFDV
                     IEANVLAIEALPAAPFDLTYCRLFLMHMQDPVATLERMMFWTKPGGVVMAQEFDFGAI
                     AVEPLCPAMAEFNRLFEGVFRGHGRNLRAGRQLPAQFEAAGLGLPDGTVADTKFLPLK
                     DLAPLLIGVYEGLFAAAAELGIADPGKASQFKQEMAEAAADGRYYCLTPILIGAWKRV
                     A"
     gene            6221..8143
                     /gene="dnaK"
                     /locus_tag="VFY74_01485"
     CDS             6221..8143
                     /gene="dnaK"
                     /locus_tag="VFY74_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5930411.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGKNPKVIENAEGMRTTPSMVAFTD
                     DGEIIVGLPAKRQAVTNPENTFFAIKRLIGRRWDDPMVEKDKKLVPYKIVKAANGDAW
                     VESHGKQYSPSQISAYILQKMKETAEAYLGQKVEQAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLEKKEGKTIAVYDLGGGTFDISILEIGDGVFEVKSTNG
                     DTFLGGEDFDMRVVDYLANEFKKESGIDLRGDKLALQRLKEAAEKAKIELSSTTQTEI
                     NLPFITADKSGPKHLTMKLTRAKLESLVEDLIQKTVGPCQNALKDAGLKPAEIEEVVL
                     VGGMTRMPKVVETVKTFFGREPHKGVNPDEVVAIGAAIQAGVLQGDVKDVLLLDVTPL
                     SLGIETLGGVFTRLIDRNTTIPTKKSQVFSTAEDNQNAVTIRVFQGEREMAADNKLLG
                     QFDLVGIPPAPRGVPQIEVTFDIDANGIVNVSATDKATGKEQSIRIQASGGLTEADIN
                     RMVKEAESHAEEDKKRRELVEVRNQAEALIHSTERMLKDYGDKVAAGDKSAVEAAVAD
                     LKSVVQGEDKDAIKQKVDALAQASMKLGEAMYQASQGGQAADGGPSASTGGAPDDKVV
                     DVDFEEVKDDDSKKSA"
     gene            8250..9389
                     /gene="dnaJ"
                     /locus_tag="VFY74_01490"
     CDS             8250..9389
                     /gene="dnaJ"
                     /locus_tag="VFY74_01490"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5930412.1"
                     /translation="MAKRCYYDVLGVTRGSRDSEIKTAFRRLAKECHPDSCNGDPHAE
                     RRFKELNEAYDALKDPQRRAAYDRFGHAAFDPSLGGGGGHGFGADFSASMSAMFDDLF
                     GEFMGQRRPRQRSGRERGSDLRYNMEIALAEAYQGKTAQIRVPTSVTCESCSGGGAKA
                     GTAPSTCRTCNGVGKVRATQGFFTIERTCPSCQGRGETIETPCPSCNGTGRVTRERTL
                     SVTIPAGVEDGTRIRLAGEGEAGLRGGPPGDLYIFLSIKPHEFFQRDGADLFCRVPIA
                     MTTAALGGQIEVPTIGGGRTRVKIPEGTESGKQFRLKDKGMPVLRSRQQGDLYIQVEV
                     ETPKNLSRKQRELLKAFEEASNASTSPASTGFFARVKEFFEGIRE"
     gene            9462..10037
                     /locus_tag="VFY74_01495"
     CDS             9462..10037
                     /locus_tag="VFY74_01495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5930413.1"
                     /translation="MRLLFFAGSIREGSFNRKLASLAHHIAAANGIEAVLAELKDYPM
                     PLYNGDLEAKEGPPEQSRAFKALLSEYQGVFIASPEYNSSVTPLLKNTLDWVTRVREK
                     GETGLEVFRSRVFAISGASPGYYGTMRGLLHLRQILEIGMGATVIPQQIAVPRAMDAF
                     EQDGSLKDKAQQTLCKRVVEALAVAARKFAR"
     gene            10040..10741
                     /gene="pyrF"
                     /locus_tag="VFY74_01500"
     CDS             10040..10741
                     /gene="pyrF"
                     /locus_tag="VFY74_01500"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5930414.1"
                     /translation="MLAKDRVIVALDVPDIEQARSLIETLGDSAGVYKIGLELLISGG
                     FALAEELARKGRAVFVDAKLSDIETTVERATAAIARLGAAFLTVHALDNKTLAAAVRG
                     RGDSALKLLGVTVLTNLASSDLIEQGIDHPLSELVLHRGTLAKKAGLDGVIASGREAA
                     EIRRALGPDVLIVTPGVRPQGSEAQDQARAVTPKEAILAGADYLVIGRPITRAPDPRA
                     AADAIIREIAAALPR"
     gene            complement(10794..11057)
                     /locus_tag="VFY74_01505"
     CDS             complement(10794..11057)
                     /locus_tag="VFY74_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5930415.1"
                     /translation="MSGQELLIILLVGLIAGWLAGQIVQGTGFGLIADIALGIVGALI
                     GAWLLPQLGIHLGSGIVAAIIAATIGALVVLLILRLVYRGGRW"
     gene            complement(11279..11590)
                     /locus_tag="VFY74_01510"
     CDS             complement(11279..11590)
                     /locus_tag="VFY74_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5930416.1"
                     /translation="MLLLLGFIAGVIWLYIYVVIASAIFSWLIAFNVVNTHNRFVYQV
                     ADMLYRLTEPALRPIRRFIPNLGGIDISPVILILFLMFITNVILPNIAVYFLEPPQPE
                     M"
     gene            11871..11946
                     /locus_tag="VFY74_01515"
     tRNA            11871..11946
                     /locus_tag="VFY74_01515"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11904..11906,aa:Ala,seq:cgc)
     gene            complement(12005..12157)
                     /locus_tag="VFY74_01520"
     CDS             complement(12005..12157)
                     /locus_tag="VFY74_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930417.1"
                     /translation="MNAFEAAEKNGRAGDLQKELEALFSRENKSPNRNATSIPASFLR
                     VTVVPN"
     gene            complement(12154..12828)
                     /locus_tag="VFY74_01525"
     CDS             complement(12154..12828)
                     /locus_tag="VFY74_01525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5930418.1"
                     /translation="MTSTNPNKALWEKGDFTRIAESMRESGEALVGGLGITKGLRILD
                     LGCGDGTTALPAARLGADVLGVDIASNLVEAGNKRAKEQGLANCKFQEGDASNLHALK
                     DHSFDLVVSIFGAMFAPKPFDVAKEMVRVTRPGGRIVMGNWIPNDPTLVAQILKISAS
                     YAPPPPEGFVSPMMWGVESNVVERFAAAGVANEAISFARDTYTFNFPARQQSSSTPSE
                     NITVQP"
     gene            complement(12887..13804)
                     /locus_tag="VFY74_01530"
     CDS             complement(12887..13804)
                     /locus_tag="VFY74_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5930419.1"
                     /translation="MNEISKRVKPVAPAVVITGATQGVGRALAEEFARGGHTLFLVAR
                     DEAALAATAKALAKAHNVAVKFVAADLCTLRGCDAVEEALGRGGLYADILVNNAAIMT
                     AGFFQDQNPATLRQAVDLNARAVADLSRRFLPGMIARRRGGILNVASVEGFMPVPYQA
                     IYAATKAFVLSFTRALAYETIFTGVRVSALAPGAVVTTIHAKAGAENSRYVQLFPVMA
                     AEDVARIGYRKFMRGRKTIVTGLFNRLSVFARRFTPDLLLVPAMGWLFRVRDAEGNLQ
                     LPRALAERKGAKGHARTDAASGDDPSLAA"
     gene            14046..14954
                     /locus_tag="VFY74_01535"
     CDS             14046..14954
                     /locus_tag="VFY74_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930420.1"
                     /translation="MRRRRSPKRSGRLTPNIATRPPSRATTARRRSRLGGGEAETAPT
                     TREIAQGPIKAVLSYVEETGEDAEVMRTPAVTVFAESKEVAKLNGESIGFADPPVGVQ
                     IAELDGGNPYPEVVVSFYTGGAHCCSATSVLTASPDGSKWTTVDLGQFDGGPLLATDL
                     NRDGRYEFMIRDNVFLYAFACYACSEAPLKLLAIDNGTVKDVTGDARFAPAHAAWLKT
                     MIENVPEEGSDVNGFLAGYVGEKILLGEGKQGWDLMLAHYDRESDWGLEICDKPLDEN
                     GECPVETLRLTFPDALERMLKEMVIR"
     gene            15072..>15178
                     /locus_tag="VFY74_01540"
     CDS             15072..>15178
                     /locus_tag="VFY74_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5930421.1"
                     /translation="MAPRAFWTGHLRLSLVNIPVKLYPATTSERRLEFH"
BASE COUNT         2808 a         4849 c         4908 g         2613 t
ORIGIN      
        1 tcaccatctc ctcatgatcg gtccgccagt tccggcaaat cgatgctggc gcggcggctc
       61 ccctcgatcc tgccgccgct cgagccggcc gagatgctcg aagtcagcat gatcaggagc
      121 ctggccggcg atctcgccgg cgggccgcgc gccgcatcat tcggcgagca tggcggcgct
      181 cgtcggcggc ggcacgcgcc cgcgtccggg cgaagtggca ctcgcccatc tcggcgtgct
      241 ctttctcgac gagctgcccg agttccaccc gcaggttctc gacgcgctgc gccagccgct
      301 cgagagcgga gagacggtga tcgcccgggc caaccatcgc atcacctatc cgtcgcgcgt
      361 tcagcttatc gcagcgatga atccttgccg ctgcggccgc gcgggcgagc ccggctatgt
      421 ctgccggcag ggcagagcct gcgccgagcg ctatcaggcg cgcatttcgg gtcccctgct
      481 cgaccgcatc gacctccaga tcgaggttcc cgccgtctct gctgccgacc tcgtgctgcc
      541 gcaagcctcg gaagggagcg ccgaggtgcg ggagcgcgtc acgctcgccc gccgcattca
      601 gatcgagcgc tacgcggcac tcggtctgcc gcgcacccgc accaacgccg aatgcgacgg
      661 cgcgctcctc gaagagatcg cagcgcccga ccgcgccggg ctcacgctcc tgcgcgaggc
      721 ggccgatgcg ctctcgctca gcgcccgcgg ctatcaccgg acccttcgcg tcgcccggac
      781 gcttgccgat ctcgacggcg aggagaaggt cgcacgcatc cacgtcgccg aggcgttgag
      841 ctatcgcggg gaaaccttgc ggcgcgccca ggaagcggcc tagccttggc ggcagcgccc
      901 cgatagcgcc gcaagtttcg gccagattga atggtgtacg ttccgcgcat gccgcgaaca
      961 actgcgtatg gaatttgctc gttccgaggg tgcgggtatg gcctccgaca gcgatgagct
     1021 tgaagagcag ctccgggaga acctgaagct aagacgcgaa cttggggcgg aggtggcgaa
     1081 ggcggcaagc cggcgcgctg ggatggctta ccgattgggc cgggttcttt acctcgcgtg
     1141 tttcggactt gccgtcttgt gcgttgcggt ctggatcggc cggttgttca ccgacttcgc
     1201 cccgatgcca atcttgcttc agcttttggc ttttgggctg cctgcactat tattttacgg
     1261 gcttggccgg gccttccgct acgtgctctc cggcgagtag ccgagggtga actgctcgtt
     1321 ggctgcggcg ggtccgattg ccggattttc ccgtgaaaac atcacgcatc gtgacgcgcg
     1381 gcgagggtgc cgtaccatcg tcatatagaa ttcgaattgg tttgctaacc ggcgcttgtt
     1441 atggccgaat cagggcgccc ggggcagggg tggtgccggt cgcccgccgg ggccctcaaa
     1501 acgcacgtaa tcgaggaggt tgttaggctg cccatgccga tgaagtcagg tcaagaacgc
     1561 cttgcaggca gatttcgcga gggtgccgcc ttcccaggct tgatgttcaa gccggtccag
     1621 gccggacggc cgctcaaggg gcggcgccgt ttcgctgtcg gaagcttcag ccggcgtttc
     1681 cagccgccca agatctatgg gcggctgggc tcgcttgaag tgcgcctcgc ccgcaagaag
     1741 agcgagatca ggcgcgctca gcggctccgc tacaaggtgt tttacgaaga gatgtcggcc
     1801 aaaccgaatg cgctcgccat cctctcgcga cgcgacgagg acgtctacga tcagatcttc
     1861 gaccaccttc tcgtgatcga ccgcgccaac cagagcaagg ggcgctggcg ccggtccaaa
     1921 gtcgtcggca catatcgcgt cctccgcaac gaggtggcgg acctttatga cggcttctac
     1981 agccaagggg agtacgacgt cgcggcactg atcgcggcca agtccccgga ttgcagtttc
     2041 atggagctag gccgctcctg cgtgctcaag ccctatcgca gccggcgtgc cctcgaactt
     2101 ctgtggcagg gcatcatggc ctatgtgcgc gagcatggcg tcgacgtcat gatcggctgc
     2161 gcgagtttcg agggcatcga tcccaacgcg cacgccgagg cgctgagctt cctctaccac
     2221 aacgcgctcg cgccgaaaga gtggcgggtc agagcccatg aacatctgcg tatcgacatg
     2281 aacatgatgc ccgaagcggc cgtctcgcac cgggccgcgc tcaaagctct gccccctctg
     2341 atcaagggct atctccgggc cggcgcctat gtcggcgacg gcgccgtgat cgatcaccag
     2401 ttcggcacca tcgacgtttt catcatcctt ccggtcgcgg cgatccaacg gcgctattcc
     2461 gagcatttcg gcgcccgcga cacgctcgcc tcctcgcttg cgcccgagtt cgcgccgctc
     2521 aactagcccc cgggagctcg cgcgcctacc caagcgtcga gcctgctttc cgggttccgg
     2581 tttgacgccc ccgcgcctca tgcctagatt cgtccatcat gcgtcatgcc gacccgatcg
     2641 agacaaagtc cgcggccgag gccgcggcgt cgctccggca gctcaacgag cgttcgcgcg
     2701 agatcttcaa gcgcatcgtc gagagctatc ttgagaccgg cgagcccgtc ggctcgcgca
     2761 acttgagccg cgctctgccc atgacgctgt cgcccgcctc ggtgcgcaat gtcatgtcgg
     2821 acctcgagag tctcggcctc atctatgcgc cgcatacgag cgcggggcgg ctgccgacgc
     2881 agacgggcct gcgtctcttc atcgacgggc tcctggaagt cggcgacatc agcgtcgaag
     2941 agcggaaaga aatcgagagc cgcatcgccg gaggcgggcg ccggcgggcg atcgaggact
     3001 tgctcggcga ggcgggcgag atgctgtcgg ggctgtcgcg ctgcgcgggc gtggtgctgg
     3061 tcgacaagca gagcaagaga ctgaagcagg tcgagttcgt cagtctcggc gagcggcgcg
     3121 cgctcgccgt gctcgtgggc gaggagggcg acgtcgagaa ccgcatcgtc gcgctgcctg
     3181 acggcctgcc gctgtcctcg ctgcgagaag catcgaatta cctcaacgcg cgcatcggtg
     3241 gcctcactct ggccgaggcc cgcgcccgca tcgagaagga gatcgaggag cggcgcgccc
     3301 agctcgacgc gctcaccgcc agcatcgtca cagagggtct cgccacctgg tcgggcgatg
     3361 gcgacgaccg caagagcctc attgtctgcg gacggtcgca cctgctcgag gatgtgagag
     3421 cgatggagga tctggagcgc gtgcggcttc tgttcgagga cctcgacacc aagaccgacc
     3481 tgatgcagct gctcggcgtt gccgagcgcg ccgaaggggt gcggatcttc atcggctccg
     3541 agaacaagct gttctctctt tcgggctcgg cgctgatcgt gtcgccgttc gaagataccg
     3601 agcagaagat cgtcggcgtt ctcggcgtga tcgggccgac gcggctgaat tacgcgcgca
     3661 tcattccgat ggtggattat acggcgaagc tcttgggacg ccttgtccct tgatttttgc
     3721 aactttacgg tcgatatccg gttgactcct tcatgacatt ggcatcgtcc agttcttgcc
     3781 atgcctaacg acaagaccga tcagggcgag gccgaccagg atcaggcgac cgatccttca
     3841 aaggcccagc cggcaaacgg ggacccggag ccggatgctc agctcggagc cgatctcgac
     3901 gcgctgctcg ccgagaacgc ggagatgcgc gaccggctgt tgcgaaccat ggccgatatg
     3961 gagaatttgc gccggcgcac ggaacgggaa aaggccgaca cggcccgcta cgccatctcg
     4021 aacttcgccc gcgacgtctt gacggtcggc gacaacctca agaggacgat cgatcatgtg
     4081 ccgcaagagg ctgcggccca ggatcctgcg ctcaagagtt ttctcgaagg cgtcgagctg
     4141 accgaacgtg agcttctgaa tgtgctcgag cgccacggcg tgacccgcat cgagccgctc
     4201 ggcgcccgct tcgacccgaa ttgccaccag gccatgtacg aagtgcaaaa agccgacgtg
     4261 cccgaaggca cggtggtcga ggtcatgcag gaaggctatg tgatcgggga ccggtgcctc
     4321 aggcccgccc tcgtcgccgt ggccaaaggg ggcgcgaaac caacgaagcc gcaagcagga
     4381 aaaggcctgg gcgccacgcc gcccgcggct gccaacgacg attttcccga cggcggctgg
     4441 caaggcgact gaggcttttt gctctctccc gagggactgt ggcgcgatcg ggctttcgct
     4501 gctctcttcc tctgtcttcg ctcacttcgg cttcgtgacc cggcgcaagg tgagattgaa
     4561 acggccacct tcgggaatga gatccgaggt gccggcgagg atgcggtcga tgccgtgata
     4621 ggcgagccgg tcagtaccgc cgagcacgac gacatcgccc gacttcagct cgaatgtccg
     4681 cgttgggtct cgacgcgact ttccgcccgc gcgaaagatt ccggtatcgc cgagcgacac
     4741 ggaaagaacc ggagcggcga aatcctcctc gtccttgtcc tggtgcaagc ccatcttcgc
     4801 gttgccggcg taataattga cgaggcaggc ttcgggcggc gccgcatagg ccgccacgtc
     4861 ccgccagagt tcgagcagca tgtttgggat cggcggccac ggtcttcccg tcttaggatg
     4921 ggtcgcctgg taacgatagc cgccttcctt gtcggtcacc cagccaagcg ggccgcaatt
     4981 cgtcatgcgc accgacatgg gcgtgccgga acgcggcatg gtcggggtaa aaagcggcgc
     5041 ttcggcaatg attccccgga tcgcggcgag aagcgcgatt tgaggctctc gtccgagcgc
     5101 ctgcggaaaa aagtccatgt tgcctttgtc tgatcgcaag cctgtcctac gctctgacat
     5161 atagcgagag cgggaaaaaa gaggagacga tcccggtggc ggcaagggcc caaaaacaaa
     5221 accccgacgg ctatgtgcat gcaaaggacg gcaccgagta tcagcgcctc cgcgcgcagg
     5281 cgctgttgtg gcaaagcgcg accgaagcgg tcctcgaccg cgttgggctc aaagccggca
     5341 tgagctgcct cgacgtcggc tcgggcccgg gcgcagtgat gcggctgatg gccgacaggg
     5401 tcggaccggc gggccgcatc accggaatcg agatggacgc cgcgcttggc gcccaggcgc
     5461 tcgccgagct tcgcgcgcaa gggggctcca cgttcgacgt gatcgaagcc aacgtgctcg
     5521 cgatcgaggc gctgcccgcc gcgcccttcg acctcaccta ttgccggctc tttctcatgc
     5581 acatgcagga ccccgtcgcg acgctcgagc ggatgatgtt ctggaccaag cctggcggag
     5641 tggtgatggc acaagagttc gatttcggcg cgatcgccgt cgagccgctc tgtcccgcca
     5701 tggccgagtt caatcgcctg ttcgagggcg tgttcagggg ccatggccgc aacttgcgcg
     5761 ccgggcggca gctccccgct cagttcgagg cggcaggcct cggccttccc gacggcaccg
     5821 tggcggacac gaagttcctg ccgctcaagg acttggcgcc gttgttgatc ggcgtttacg
     5881 agggcctgtt cgccgccgcc gcggagcttg ggatcgccga tccggggaag gcgagccagt
     5941 tcaagcagga gatggcggag gccgccgccg acgggcgcta ttactgcctc accccgatcc
     6001 tcatcggcgc ctggaagcga gtcgcgtgac gcgctcgctt caggcgcgct cgtctcgccc
     6061 gcttctcctt gcgggccata gggttgccac ctatataccg cgggaacgcc gccgcgtgtg
     6121 tcggcggacg ccgaatattg gggaccgacg ccatgcttcg cgctgaaatg gcgggtgccg
     6181 ttcgcgggcc cgcacggtct gccagaaaga gaggttcgta atggcgaaag tgatcggaat
     6241 cgatctcggc acgaccaatt cctgcgtcgc cgtgatggag gggaagaatc cgaaggtcat
     6301 cgagaacgcg gaaggcatgc gcaccacgcc gtcgatggtg gcctttaccg acgacggcga
     6361 gatcatcgta ggacttccgg ccaagcgcca agccgtcacc aatcctgaaa acacgttctt
     6421 cgccatcaag cgcctgatcg gccgtcgctg ggacgacccc atggtcgaaa aggacaagaa
     6481 gctcgtcccc tacaagatcg tgaaggccgc caatggcgac gcctgggtcg agtcgcacgg
     6541 caagcaatat tcgccctcgc aaatctccgc ctacattctc cagaagatga aggagaccgc
     6601 cgaagcctat ctcggccaga aggtcgagca ggcggtcatc accgttcccg cctatttcaa
     6661 cgacgcccag cgccaggcga ccaaggacgc cggcaagatc gcggggctcg aggtgttgcg
     6721 catcatcaac gagccgacgg cggcggcact cgcctatggg ctcgagaaga aagagggcaa
     6781 gaccatcgcc gtctacgatc tcggcggcgg cacgttcgac atctcgatcc tcgagatcgg
     6841 cgacggcgtg ttcgaggtga agtcgaccaa tggcgatacc ttcctcggcg gcgaggattt
     6901 cgacatgcgc gtcgtcgact atctcgccaa cgagttcaag aaagagtcgg gcatcgatct
     6961 gcgcggcgac aagctcgcgc tccagcgttt gaaagaagcg gcggagaagg cgaagatcga
     7021 gctgtcctcg accacccaga ccgagatcaa tctcccgttc atcacggcgg acaagtccgg
     7081 gcccaagcat ctcaccatga agctcacgcg cgccaagctc gagagcctgg tcgaggatct
     7141 catccagaag accgtcggcc cgtgccagaa cgcgctcaag gatgccggcc tcaagccggc
     7201 cgagatcgag gaggtggtgc tggtcggcgg catgacgcgc atgcccaagg tcgtcgagac
     7261 ggtgaagacc ttcttcggac gcgagcccca taagggcgtg aaccccgacg aggtcgtggc
     7321 catcggcgcc gccatccagg ccggcgtgct gcaaggcgac gtcaaggacg tgctcctgct
     7381 cgacgtgacc ccgctctcgc tcggtatcga gaccttgggc ggcgtgttca ctcggctcat
     7441 cgatcgcaac acgacgatcc cgaccaaaaa gagccaggtg ttctccaccg ccgaggacaa
     7501 tcagaacgcg gtcaccattc gcgtcttcca gggcgagcgc gagatggcgg ccgacaacaa
     7561 gctgctcggc cagttcgatc tcgtcggcat tccgcccgcg ccgcgcggcg tgccgcagat
     7621 cgaggtgacc ttcgacatcg acgcgaacgg catcgtcaat gtctcggcca ccgacaaggc
     7681 gaccggcaag gaacagtcga tccgcatcca ggcctctggc ggcctcaccg aagccgacat
     7741 caatcgcatg gtgaaggaag ccgagagcca cgccgaggag gacaagaagc gccgcgagct
     7801 ggtcgaggtg cgcaaccagg ccgaggctct gatccattcg accgagcgga tgctcaagga
     7861 ctacggcgac aaggtcgcgg ccggcgacaa gagcgcggtc gaggccgcgg tggccgatct
     7921 caagtcggtc gtccagggcg aggacaagga cgcgatcaag cagaaggtcg atgcgctggc
     7981 gcaagcctcg atgaagcttg gcgaggccat gtatcaggcg agccagggcg gtcaggccgc
     8041 ggacggcgga ccgtcggcct ccaccggcgg cgcccccgac gacaaggtgg tcgatgtcga
     8101 tttcgaagaa gtcaaggatg acgacagcaa gaagtcggcc tgacgagatg gcaaggcgaa
     8161 ggcgccaggc cgaggaggcc cggcgccgaa gccgaagaga atgggcttag gcgcgccccg
     8221 gcgggggagc gccgaagtcc ggaggggcga tggcaaaacg gtgctactac gacgttcttg
     8281 gtgtgacgcg cgggtctcgc gacagcgaga tcaagacggc gtttcgccgg ctcgccaagg
     8341 aatgtcatcc cgacagctgt aacggcgacc cccacgccga gaggcgtttc aaagaactca
     8401 acgaagccta cgacgcgctg aaagacccgc agcgccgtgc cgcttacgat cgcttcggcc
     8461 atgccgcctt cgatccgagc ctcggcggcg gcggcggcca cggcttcggc gcggatttct
     8521 cggcctcgat gtcggcgatg ttcgacgatc tgttcggcga gttcatgggt cagcggcgcc
     8581 cgcgccagcg ctcgggccgc gagcgcggct ccgatctccg ttacaacatg gagattgcgc
     8641 tcgccgaggc ctatcagggc aagacggcgc agatccgcgt gccgaccagc gtcacctgcg
     8701 agagctgctc gggcggaggc gcaaaggccg gcaccgcacc gagcacctgc cgcacctgca
     8761 acggcgtggg caaagtgcgc gcgacccaag gcttcttcac catcgagcgc acctgtccct
     8821 cgtgccaggg acggggcgag accatcgaga cgccgtgccc gtcttgcaac ggcaccggcc
     8881 gggtcactcg cgagcgcacg ctctcggtca ccattcccgc cggcgtcgag gacggcacgc
     8941 gcatcaggct cgccggcgag ggcgaggcgg gcttgcgcgg cggaccgcca ggcgatctct
     9001 acatcttcct ctcgatcaag ccgcacgaat tcttccagcg cgacggcgcc gatctcttct
     9061 gccgggtgcc gatcgccatg accacggcgg cgctcggcgg ccagatcgaa gtgccgacca
     9121 tcggcggcgg caggacgcgg gtgaaaatcc cggaaggcac cgaaagcggc aagcagttcc
     9181 gcctcaagga caaaggcatg ccggtgctgc gctcgcggca gcagggcgac ctctacatcc
     9241 aggtcgaggt cgagacgccg aagaacctgt cgcgcaagca gcgcgaactg ctgaaagcct
     9301 tcgaggaggc ctcgaacgcg agcacgagcc cggcctcgac cggcttcttc gcccgcgtga
     9361 aggaattctt cgagggcatt cgcgagtagg cgctaccctt ttcctcacgc cttgacgtag
     9421 agtgcccggc ggagcgcgcg ctcgccaagg aaaccccgac gatgcgactt ctcttcttcg
     9481 ccggaagcat ccgggaaggc tcgttcaacc gcaagctcgc gtcccttgct catcacatcg
     9541 cggcggcgaa cggcatcgag gcggtgctgg cggaactcaa ggactatccg atgccgctct
     9601 ataacggcga cctcgaagcg aaagaggggc cgcccgagca gtcgcgcgcc ttcaaggcgc
     9661 ttctgtccga atatcagggc gtctttatcg caagtcccga atacaattcc tcggtcaccc
     9721 cgctcctcaa gaacacgctc gattgggtga cgcgcgtgcg cgagaagggc gagaccgggc
     9781 tcgaagtctt ccgctcgcgc gtgttcgcca tttcgggcgc ctcgccaggc tattacggga
     9841 ccatgcgcgg cctgttgcat ctccgccaga tcctcgaaat cggaatgggc gcaaccgtca
     9901 ttccgcagca gatcgccgtg ccgcgcgcca tggacgcgtt cgagcaagac gggagcctca
     9961 aggacaaggc gcagcagacg ctttgcaagc gtgtggtcga ggcgctcgcc gttgccgcaa
    10021 ggaaattcgc acggtgacga tgctcgccaa ggaccgtgtg atcgttgcac tcgatgtgcc
    10081 ggatatcgag caggcgaggt ccctgatcga gacgctcggc gacagcgcgg gcgtttacaa
    10141 gatcggtctg gagctcctga tctccggcgg atttgcgctg gctgaggagc tcgcgcgcaa
    10201 gggccgcgcg gtgttcgtcg acgccaagct ctccgatatc gagacgaccg tcgagcgggc
    10261 gacggccgcg atcgcccggc tcggcgctgc gtttctgacg gtccatgcgc tcgacaacaa
    10321 gacgctggcg gccgcggtcc gcgggcgcgg cgacagcgcg ctcaagctcc tcggcgtgac
    10381 ggtgctcacc aaccttgcgt cttcggactt gatcgagcag ggcatcgatc atcctctgtc
    10441 cgagctcgtt ctgcatcgcg gcacgcttgc gaagaaagcg ggcctcgacg gcgtgattgc
    10501 ctcggggcgg gaggcggccg agatccgtcg cgcgctagga cccgatgtcc tgatcgtgac
    10561 gccgggggtc aggccgcaag gaagcgaggc tcaggaccag gcccgcgcgg taacacccaa
    10621 ggaagcgatc cttgcgggcg ccgactatct cgtgatcggg cgtccgatta cccgcgcgcc
    10681 cgatccgcgg gccgccgccg atgccattat tcgtgagatc gccgcggccc taccgcgtta
    10741 gcgccgcaag gcgagcttcg atcaggccaa gcgagcggcc tggcgttgcc ttcctaccag
    10801 cgccctcctc ggtagacgag gcgaagtatc agcagcacca ccaatgcgcc gatcgtcgct
    10861 gcgatgatgg cggcgacaat gccactaccg agatggatgc cgagctgtgg caacagccac
    10921 gcgccgataa gcgcgcctac gatgcctaag gcgatatcgg cgataaggcc gaagcccgta
    10981 ccctgcacga tttgtccggc aagccagccg gctatgagac cgacgagcaa gatgatgaga
    11041 agctcttgtc ctgacattgt ctcccctctt ggcgaattcc tcagtacgcc ttatgagaag
    11101 ctcttgtcct gacattgtcc cccctcttgg cgaattcctc agtatgtcgc cttaggagaa
    11161 taccccgtca tcaatcaagc tgaaacggga gtggggggtt atgcaccgcg tcgggcgtca
    11221 gccgcacctt aagcgtcatg ccggaggcag tttgaaggat cgcaggcaaa ggctgcgatc
    11281 acatttcggg ctgcggcggc tcgaggaaat agacggcgat gttgggaagg atgacgttgg
    11341 tgatgaacat gaggaacaag atgagaatga ccggcgagat atcgatgccg ccgagattgg
    11401 gtatgaaccg cctgatcggc cggagcgcgg gctcggtcag ccggtacagc atgtcggcca
    11461 cctgatagac gaagcggttg tgggtattga ccacgttgaa ggcgatcaac cagctaaaga
    11521 tggcgcttgc gatcacgacg tagatgtaga gccagatcac gcctgctatg aagccaagca
    11581 ataggagcat catgtcctcc tcggtactga ggctcatgta gcggacaggc ggcgatcagg
    11641 gcaagagtca ggtgccaggt atcaggtatc aggtgtcagg tgtcaggtgt cagaaatcag
    11701 aagtcagaga tcagggaacg gttgcccctt gaaatctgag atcaggtgcc aggtgccagg
    11761 tggcagaaat cagaagtcag aaatcaggca atggctctcg gttgaacacc ggcccagcag
    11821 cttgcccctt gaaatctgat atctgatacc tgatacctga cacctggaat ggggccgtag
    11881 ctcagttggg agagcgcagc aatcgcactg ctgaggtcac cggttcgatc ccggtcggct
    11941 ccaccatccc ccagatcaag attggttggt gacaggccga tgcgatggcg cgcgggttcg
    12001 gggactaatt gggcacaacg gtgacgcgca agaagctcgc aggaatggag gtggcattcc
    12061 tgtttgggct tttgttctcg cggctgaaca gggcttccag ctccttctgg agatcgccgg
    12121 cgcgaccgtt cttttccgcc gcttcgaagg cattcatggt tggaccgtaa tattttcgga
    12181 aggcgtcgac gaactctgct ggcgggccgg aaaattgaag gtgtaggtgt ctctggcaaa
    12241 cgatatcgcc tcgtttgcaa cccccgcggc cgcaaagcgc tcgaccacgt tactttcgac
    12301 tccccacatc atggggctga cgaagccttc cggcggtggc ggcgcgtagg acgcgctgat
    12361 tttcaggatt tgcgccacca aggtcggatc gtttgggatc cagttcccca tcacgatgcg
    12421 gcccccgggc cttgtcacgc gcaccatctc cttggccacg tcgaacggct tcggcgcaaa
    12481 catggcgcca aagatgctca cgacgaggtc aaagctgtga tctttcagcg cgtgcaaatt
    12541 cgacgcatcg ccctcctgaa acttgcagtt ggcgaggccc tgctccttcg cacgcttgtt
    12601 tccggcctcg acaaggttgc tcgcgatgtc gacgcctagc acgtcggctc caagtctcgc
    12661 tgccggcaac gccgtcgttc catcgccgca cccaagatcc aaaatcctga gccctttggt
    12721 gattccgagc cccccgacca gcgcttcccc gctctctctc atactttccg cgatgcgcgt
    12781 gaagtcgccc ttttcccaaa gtgccttgtt tggattggtt gatgtcatcc gcatccgcct
    12841 cactgtgccg cgatggcgga ccttagcgtg ctaatacttg gattgcctag gcggcgaggc
    12901 tcgggtcgtc acccgaagcg gcgtcggtgc gcgcgtgacc cttggcgcct ttgcgttcgg
    12961 caagcgcgcg cggcaattgc agattgcctt ccgcgtcgcg caccctgaaa agccatccca
    13021 tcgccggcac gagaagaaga tccggcgtga agcgccgggc gaagacgctc aagcgattga
    13081 acaagccggt cacgatggtc ttccggccgc gcatgaactt gcggtacccg atgcgggcga
    13141 cgtcctccgc ggccatcacc ggaaagagtt ggacatagcg ggaattttcc gcgcccgctt
    13201 tggcgtgaat cgtggtcacg accgcgccgg gcgcaagcgc cgagacccgc acgcccgtga
    13261 agatggtctc gtaggcgaga gctcgggtga aggagaggac gaaggccttc gtcgcggcgt
    13321 agatcgcctg gtacggcacc ggcatgaagc cttcgacgga agcgacgttg aggattccgc
    13381 cgcggcgccg cgcgatcatg ccggggagaa agcgccgcga gagatcggcc accgcgcgcg
    13441 cattgagatc gacggcctgc ctgagcgtcg ccgggttctg gtcttggaag aatcccgcgg
    13501 tcatgatcgc ggcattgttg acgagaatgt cagcgtaaag accgccgcgc cctagcgctt
    13561 cctcgacggc gtcacagccc ctgagcgtgc agagatccgc cgcgacgaac ttgactgcga
    13621 cattgtgagc cttggcgagc gccttggcgg tcgcggcgag cgccgcctca tcgcgcgcga
    13681 caagaaacag cgtgtggccg ccgcgcgcga actcctcggc gagcgccctg cccacccctt
    13741 gcgtcgcccc ggtaatgacg accgcggggg cgacaggttt aacccttttg ctgatttcgt
    13801 tcatcgccaa tcccgaccgc ttcaacaaaa cgcagcttta ggctgaaagc gcctcgcccg
    13861 gcgccgccaa gcggcaagcc caacgataat atgggaccgc caggctctcc cggctagaat
    13921 agccgacgcg ctttttgcgt gcgagccttc attgtgttta gcgagagatc ccgtgggccg
    13981 gacatccttg ccgctgcgcc tttcgctgtt tgcagccttc atcctcgcct cacaaggcgg
    14041 cgccgatgcg gcgccgacgc tcgccgaagc ggtcagggag gctgacgcca aatatcgcga
    14101 cgcgcccgcc atcgcgggcg acgacagcgc gccgaagatc gaggctgggc ggcggcgagg
    14161 cggagaccgc gcccacgaca cgggaaatcg cgcaagggcc gatcaaggcg gtgctgagct
    14221 acgtcgaaga gacgggcgag gacgccgagg tcatgcgcac gccggccgtc accgtcttcg
    14281 ccgagagcaa ggaggtcgcc aagctcaacg gcgagagcat cggcttcgcc gatcccccgg
    14341 tcggcgtgca gatcgccgag ctcgacggcg gcaatcctta cccggaggtc gtggtgtcgt
    14401 tctacaccgg aggagcgcat tgctgctccg ccaccagcgt tctcaccgca agccccgacg
    14461 gctcgaaatg gacgacggtc gatctcggcc agttcgacgg cgggcccttg ctcgcgaccg
    14521 atctcaaccg cgatggccgc tacgagttca tgatccgcga caatgtcttt ctctacgcct
    14581 tcgcctgcta cgcctgctcg gaagcgccgc tcaagctcct tgcgatcgac aacgggaccg
    14641 tcaaggacgt gacgggagac gcgcgctttg cgccggcgca tgcggcctgg ctcaagacca
    14701 tgatcgagaa cgtcccggag gaggggagcg acgtgaacgg attcctcgcg ggctatgtgg
    14761 gcgagaagat cctgctcggc gaggggaaac agggatggga cctgatgctc gcccattacg
    14821 accgggagtc ggactgggga ctcgaaatct gcgacaagcc gctcgacgaa aatggggagt
    14881 gcccggtcga aaccttgagg ctcacttttc ccgacgcgct cgaacgcatg cttaaggaaa
    14941 tggttataag gtagagaaat gaggttccca ccgccggatt ccacgcgaat ctgcggattt
    15001 tcgctttcgg ttgacccgat ccgggcctgg tctccggccc tttgatcggc atttttcgag
    15061 gagtaaggag tatggcaccg cgcgcgttct ggaccggcca tttgcggctt tccctcgtca
    15121 acattccggt caagctctat ccggcgacca cctcggaacg ccggctcgag tttcacca
//
LOCUS       DASUQP010000031         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1104928, whole genome shotgun sequence.
ACCESSION   DASUQP010000031 DASUQP010000000
VERSION     DASUQP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104928"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..724
                     /locus_tag="VFY74_01545"
     CDS             191..724
                     /locus_tag="VFY74_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930422.1"
                     /translation="MMVTITNAHVAPPPVRHDGVVLAKYHPSENASAAAALKGKTLPA
                     PHHDDNKVPSVAVIVSIADQRIVVVEHDRTVAEGKAFIANPGKPLGSHVFVLMGSGTY
                     GQGLLWQVISHNATPGGVVSGADDELIQRISGEPSLVHAMKAGHEPGHGACPDRRADH
                     GENRSGAGFSIITADER"
     gene            639..1928
                     /locus_tag="VFY74_01550"
     CDS             639..1928
                     /locus_tag="VFY74_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5930423.1"
                     /translation="MVLVPTDGPITAKTAAGLDFQSSPQTSVETVSNRLNATPGAAWP
                     GCGIVATIAAARPAHVQKSSRRIASEKVEDPHASGPPLDVWGWAERVSIIGLFILAIC
                     YAAFVTQQILVPVILAWVVGAILRPVVAWAEDFGIPRVVAVITVALVALVIVLCIIGL
                     LSTPFTYWIGRTTDLASLIKEKLQLLSHPLAIFDAIDHALAEISGGTAHASIPAFDTS
                     SIVRSIIATLTPAVTEFLLFFFAMIFSMLYANEIKAGIAFVFSGNQARRAARCALDDA
                     ESNLSRYFGTLAVVNLCLGTIAAALAWAVGLPHPLLWGVLAAALNFVPYLGPALMVAT
                     LFVIGLMSFATLGHALIAPLAWIGVTTLEGQFITPTIIGHRMTLNPFLVFLSIAFWAW
                     MWGPLGAFLAVPLLITAVVIGRHLSPQKHMAVQAAGE"
     gene            1963..2667
                     /locus_tag="VFY74_01555"
     CDS             1963..2667
                     /locus_tag="VFY74_01555"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX5930424.1"
                     /translation="MFEDPLFWVFVLPGLLLGLYAQSRIKLNITKYAEVRTQNGITGA
                     EVARRLLDAKGLENVAIESTPGVLSDHYDPRSKTLRLSQQVYFAPSVAAAGIAAHEAG
                     HALQDAEDYFPMEARTYIVPVVQLASQIAPWLFISGLVLQLETLTWAGVILFGSSFLL
                     ALLTLPVEFNASARAKELLVSHGIIKGDEQLAGVERVLGAAAWTYVAAAVSAIGVWLF
                     YVFVLFFRGSSESGHR"
     gene            complement(2741..3643)
                     /locus_tag="VFY74_01560"
     CDS             complement(2741..3643)
                     /locus_tag="VFY74_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5930425.1"
                     /translation="MAESIFPGYDNRLVPVGDQRIGVHVGGTGAPLLLLHGYPQNHAA
                     CIKLAAPLARNFTCVIADLPGYGASSIPADAKDHEPFSKRRMAAVLVSAMASLGHARF
                     SVMGHDRGARVAYRMALDHPATIRRVAIIEIIPTSDMWDAFNAEMAMKAYHWSFLAQP
                     FPLPETLIAADPDFYLEWTLRSWSKGRSLEAFDPRSLQSYREQMRDPQRVHAMCEDYR
                     AGAFIDRSLDLADRALGAKITAPLLFVWSRSGFPAAARDPLSLWRSWSDNLSGAEIDS
                     GHFMPEENPDALSAAAVPFLAADH"
     gene            complement(3710..4405)
                     /locus_tag="VFY74_01565"
     CDS             complement(3710..4405)
                     /locus_tag="VFY74_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5930426.1"
                     /translation="MPVTLITGANRGLGLEFARQYLADGWQVYAACRDPASASELRRL
                     AQDSGGKLRILAMDVTDPRSIRAAATELEGQAIDLLLNNAGIIGPRGQAIGNIDYDAW
                     AEVLAVNTMGPMRVSEAFVEHVANSDLKLIVTLTSGMGSIADNTSGGSIVYRSSKAAV
                     NMVMRSLAIDLAPRGIICVVVNPGWVRTDMGGPNATVEASESIGALKRLIATLGPQHS
                     GKFFNHTGREYPW"
BASE COUNT          820 a         1472 c         1454 g          894 t
ORIGIN      
        1 ggagtactgg cggccacttg aacgcctcga tgcccggtct ctccaatcgc gagaggctgc
       61 ttgtcgccgc tgctacacat tcgcccctga ccaggacgcg gggccggcga gttgcccggc
      121 tctcccgcct cacacggctg cgtccggctc ccgaaggaat tttctgcgct gatccgtcac
      181 gcttgtcggc atgatggtga ccataaccaa cgcccatgtc gcaccgcccc ccgtccggca
      241 cgacggcgtc gtcctggcga aataccatcc ttccgagaac gcctccgcgg ccgccgcgct
      301 gaagggcaag actctgccgg cgccgcatca cgacgacaac aaagtgccct ccgtcgccgt
      361 catcgtgtcg atcgccgatc agcggatcgt cgtggtcgag cacgacagga ccgtcgccga
      421 aggcaaggca ttcatcgcca atccaggcaa gcccctgggc tcgcacgtgt tcgtgctcat
      481 gggaagcggc acgtatgggc agggtctcct ctggcaggtc atctcgcaca acgcgacgcc
      541 gggcggcgtc gtcagcggcg ccgacgacga gctcattcag cgaatcagcg gcgagccgtc
      601 gctcgtccac gccatgaaag cgggccatga acccgggcat ggtgcttgtc ccgaccgacg
      661 ggccgatcac ggcgaaaacc gcagcggggc tggattttca atcatcaccg cagacgagcg
      721 ttgagacggt tagcaatcgt ctgaatgcga ccccgggggc cgcgtggccc ggctgtggta
      781 tagtcgcgac gattgccgcc gcaaggccag ctcacgtgca aaaatccagt cgaaggattg
      841 catccgaaaa agtcgaggac ccgcacgcaa gcggaccacc gcttgacgtt tggggctggg
      901 ctgagcgcgt ctcgatcatt gggctcttta ttctcgcaat ttgctacgca gcttttgtga
      961 cgcagcagat attggtcccc gtgatcctcg cctgggtcgt cggcgccatc ttgcggcccg
     1021 tggtcgcatg ggcggaagat ttcggcatac cgagggtcgt tgccgtcatc accgtcgctc
     1081 tcgtcgctct cgtgattgtg ctttgcatca tcggcctcct gtccacgcct tttacctatt
     1141 ggatcgggcg aaccacagac ctcgcttcct tgatcaagga gaagctgcaa ctgctcagcc
     1201 atccactcgc aattttcgac gcgatcgacc acgcgctagc ggagatttcc ggcggaaccg
     1261 cgcatgcgtc catcccggcc ttcgatacct cgtcaattgt gcggtcgatc atcgcaacgc
     1321 tgacgccggc cgtaacggag tttctcctgt tcttctttgc aatgattttt tcgatgcttt
     1381 acgcaaatga gatcaaggcc ggcattgcgt ttgtcttttc cggcaatcaa gcacgcaggg
     1441 ccgcgcgatg cgccctggat gacgcggaaa gcaacctgag ccgatatttc ggcacgctcg
     1501 cggtggtgaa tctgtgtctg gggacgatcg cggcggcgct ggcgtgggcc gtcggcctgc
     1561 ctcacccgct tctgtggggt gtcttggcgg ctgcgctcaa cttcgttccc tatctcggcc
     1621 cggccctcat ggtggcaacg ctattcgtaa tcgggctgat gagctttgcg accctcggtc
     1681 acgcgctcat cgcgcccttg gcctggatcg gggtcacgac cttggagggg cagttcatta
     1741 cgcccacgat catcgggcat cgcatgaccc tcaacccctt cctcgtcttc ttgtcgatcg
     1801 ccttctgggc gtggatgtgg gggcccttgg gcgcgtttct cgccgtgccc ttgctcatca
     1861 cggcggtcgt gatcggacgg catctttcgc cgcaaaaaca catggcggtt caagccgccg
     1921 gcgagtgacg cgccggcaaa gactaagggt gggacttaga acatgttcga ggatccgctt
     1981 ttttgggtat tcgtgctgcc cggcctattg ctcgggctct atgcgcagtc acgcatcaaa
     2041 ctcaacatca cgaaatatgc tgaagtccgc acccagaacg ggattacggg cgccgaggtg
     2101 gcgcgccggc tgctcgacgc gaagggcctg gagaatgtgg ccatcgagtc cacgcccgga
     2161 gtcttgagcg atcactacga tccccgttcc aagacgttgc ggctcagcca gcaagtctat
     2221 ttcgccccga gcgtcgcggc cgccggcatt gccgcgcatg aagcggggca cgccttgcaa
     2281 gacgccgaag actacttccc catggaggcg cgaacctaca tcgtgccggt cgtccaactc
     2341 gcgtcgcaaa tcgcgccgtg gctgttcatc tcggggttgg tcctccagct cgagaccctg
     2401 acttgggccg gcgtcatcct tttcggctcg tctttcctcc tcgcgctcct cacgcttccc
     2461 gtggagttca acgccagtgc gcgggcaaag gaactcttgg tcagccacgg catcatcaag
     2521 ggcgacgaac agctggccgg tgtcgagaga gtcttggggg ccgcggcctg gacctatgtg
     2581 gcggccgccg tgtcggccat cggcgtctgg ctgttctacg tgttcgtcct gttcttccgc
     2641 gggagctccg agtccggcca cagatgaaat cctaaaggga tctcacgctc gggtgagcga
     2701 ccgcttgacc gcgacctagg ggaccaacct gactgacaga tcaatgatcc gcagccagga
     2761 acgggacggc cgcggccgac agagcatccg ggttttcttc cggcatgaag tggccggaat
     2821 cgatctcggc gccggagagg ttatcgctcc agcttctcca caggctgaga gggtcgcgcg
     2881 ccgcagccgg gaagccggac ctcgaccaaa cgaaaaggag cggcgcggtt atcttcgcgc
     2941 cgagtgccct gtcggcgagg tcgagcgacc tgtcgatgaa ggcgccggcg cggtaatcct
     3001 cgcacatcgc gtgaacgcgc tgagggtcgc gcatctgctc gcgatagctt tgcagcgatc
     3061 gcggatcgaa cgcctcgagc gagcggccct tgctccagct ccgcaacgtc cattcgagat
     3121 aaaagtcggg atctgcggcg ataagcgttt cgggcaaggg gaacggctga gccaggaacg
     3181 accaatggta tgccttcatc gccatttcgg cgttgaacgc gtcccacatg tcgctggtgg
     3241 ggatgatctc aatgatcgca acgcgccgga tcgttgccgg atggtccaag gccattcggt
     3301 acgcgacccg cgcgccgcgg tcatggccca tcacgctgaa acgcgcatga ccgagggagg
     3361 ccatggcaga cacgagcact gccgccatcc ggcgtttgga aaacggttca tggtccttag
     3421 cgtcagcggg aatggagctt gcgccgtagc cgggaaggtc ggcgatcacg caggtaaaat
     3481 tcctcgctaa gggtgcagcg agcttgatgc acgcggcgtg gttctgcggg tagccgtgta
     3541 gaagcaggag cggtgcgccg gtgccgccga catgaacgcc aatcctttga tcgccgaccg
     3601 ggaccaatcg attgtcgtag ccaggaaaga tcgactccgc catgacctgc tcttgacaac
     3661 tgcggccggc cccagcgcgc gatgattgcc ttgctgcgat cgcgagcggc taccagggat
     3721 attcccggcc ggtatgattg aagaacttgc cggaatgctg aggcccaagg gtcgcgatga
     3781 gtcgtttcag cgcgccgatg ctttcggagg cctccacggt ggcgttcggc ccgcccatat
     3841 cggtgcgcac ccagccggga ttgacgacga cgcaaatgat gccgcgcggc gcgagatcga
     3901 ttgccaggct gcgcatgacc atgttcaccg ccgctttcga gctgcgatag acgatcgagc
     3961 cgccggaggt attgtcggca atcgacccca tgccgctggt cagcgtgaca atgagcttga
     4021 gatcgctgtt cgccacatgc tccacaaacg cttccgacac gcgcattggc cccatggtgt
     4081 tgacggcgag cacctccgcc caggcgtcgt aatcgatatt tccgatggcc tggcctcgcg
     4141 gcccgatgat gccggcgttg ttgagcagga ggtcgatcgc ttggccttcg agttccgttg
     4201 ccgccgcgcg gatggagcgc ggatcggtga cgtccatcgc caatattcgc aatttgccgc
     4261 cgctgtcctg cgcgaggcgc cggagctcgg acgcagatgc cggatcgcga caggccgcgt
     4321 agacctgcca tccatcggcc agatattggc gggcaaactc gagcccgagc ccgcgattgg
     4381 caccggtgat caacgtaacg ggcattgggg acggatgctc ctgggggaac tggacggcaa
     4441 atcacaactg gtgatgccgc gaaaggctca aagtgcggat ctggagcgcg ttgcggtcca
     4501 aatgcccgag cagagaccgg agggccctgt accggtccac tttccgctgc cctgggaccg
     4561 gccgaaccgt ccgagccctt gggcggtgcg cgggccggcc acggcattca tccggacctg
     4621 ccccgcggcg gaaacgtgcc
//
LOCUS       DASUQP010000032        18115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_969300,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000032 DASUQP010000000
VERSION     DASUQP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18115
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969300"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFY74_01570"
     CDS             <1..674
                     /locus_tag="VFY74_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930427.1"
                     /translation="GAEGLGRGHNRTGAGRRVPPYNGAMRFIAAAIACAIVLFGSAAA
                     LAQISPAEKPGAKTKPAAQKPAEKKPAAQKAAPSQPQVVMPDAEKIVLLLRTTLMTLN
                     DALQTGNFTVMRDISAPGFRDANTAARLSLSFSDLASKGVDLSPTSVIAPQLTEPPGL
                     DQKKGLLRLKGYFPGQPVQINFEMLYQAVDGRWRLFGLSVQPGPPTPPPASASAPAPG
                     AATQQ"
     gene            686..1192
                     /locus_tag="VFY74_01575"
     CDS             686..1192
                     /locus_tag="VFY74_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5930428.1"
                     /translation="MASIPARSKPSLLGRLRPNRLLTAMFRPIWRMRRGTTLGAQGAV
                     IDAEGRVLLVRHGYRPGWWFPGGGVEWGETLVSALGRELEEEVGVKLTGAPELHGIFS
                     NFASFPGDHIAVFVVRHWTRREDYRKRGEIAEARMFAWQDLPERVDPGTRARLAEIFD
                     QAPIDARW"
     gene            complement(1457..2071)
                     /gene="wrbA"
                     /locus_tag="VFY74_01580"
     CDS             complement(1457..2071)
                     /gene="wrbA"
                     /locus_tag="VFY74_01580"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX5930429.1"
                     /translation="MADTTRLLVLYHSSYGHTETLAYAVAHGARAVDDVHVSVKRVPE
                     LMPEDIMRAAGMKVDQPAAIADPQELAEYDGIIFGTPTRFGNMTSQMRNFLDQTGPLW
                     VSGALIGKVGSVFTSTSTGGGNETTIVSFHWTLLHHGMVVVGLPYSAPDLPDISEVKG
                     GSPYGAGTIAAPDGSRLPSQKELNLASFQGHHVAKIARKLAGLQ"
     gene            2415..2921
                     /locus_tag="VFY74_01585"
     CDS             2415..2921
                     /locus_tag="VFY74_01585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5930430.1"
                     /translation="MSTGKFEIRHETDGDAPVLSRLAAEAFGPGRFTRSAYRVREGVA
                     PVAGLSLCGVLDGRLVGGIRFTAIRIGDKEGGVLLGPLIVDPTAMGRGFGKALVEEGL
                     ARARAERFCLVLLVGDMPYYGRFGFTPVMPGRITLPGPVDPARLLGLELVTGAMEGAA
                     GGVKGYAR"
     gene            complement(2918..5002)
                     /locus_tag="VFY74_01590"
     CDS             complement(2918..5002)
                     /locus_tag="VFY74_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930431.1"
                     /translation="MSWSIEFIPFVPWPVLWGLAALGAVLLALLFWRSRRGAIFRLVS
                     YTLLLLAIANPHLKQEEREPLNNVVAVVVDESQSQAIAGRTARTEQVRRDLEERLKAF
                     SNLDTRWVRSTSTAADSERDGTMLFTDLAQALADVPPDRLAGVLMITDGEVHDVPAAA
                     AGLGFDAPVHALLTGKQGEFDRRLQVLSAPRFGIVGSSQDIEVKVTDTAAAGSGGAVK
                     LTVTHQGQPPVTQSVRAGDKVAIPVDINHAGPNIVEIEAEPAQGEITTVNNRAVLTVE
                     GVRENLRVLLVSGEPHAGERTWRNMLKSDAAVDLVHFTILRPPEKQDGTPINQLSLIA
                     FPTRELFQEKLDQFDLIIFDRYQRRGVLPLLYLENVARYVEKGGAVLVSAGDDYASPL
                     SLFRTPLGQVLPAVPSGRVVEEPFKPKLSELGEKHPVTSDLPGAGGDEPSWGRWFRVV
                     EADPRDAEVLMKGAQGKPLLVIGERGQGRVALLLSDHTWLWARGYDGGGPYTDLLRRL
                     AHWLMKEPDLEEETLKATSRGQTISIERRSIKDEVEKVTVTAPSGKEEVVTLDKGERG
                     LWRKAVNATEQGVYRISSGALANVVTVGNANARELSAVTATGEVLSPILEETGGGVFW
                     LGRDEAEAAKLPRLVMLRGGQVMHGDDWLGLHRRDAYHVKGVRLFPLFSGFIALGLLL
                     GFLAATWFREGH"
     gene            complement(5006..7858)
                     /locus_tag="VFY74_01595"
     CDS             complement(5006..7858)
                     /locus_tag="VFY74_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX5930432.1"
                     /translation="MITIGAIGFLQPWVLAALAALPAIWWLLRLTPPSPQLVVFPPTR
                     LLKDLKTTEETPARSPWWLTALRMLLAALIILALARPVLNPDRQNFTGSGPLLIVIDN
                     GWASGAHWPERREAIEAAIDRAARDGRTIVLAPSAPGPPVSAPLSPDKARERIGGINP
                     EPYAPARAALAATLEKELGGKTGFSVLWLSDGLDYGDGKTFSEALAKLAGDGGSLSVL
                     RPERGDAALALGQGAAESGAFAARILSGAEGPRSGVIKALTGRGEPLGETAFTIGQGA
                     RETTVQFDLPLEIRNQVARIAIAGERSAGAVSLLDARSQWHRVGIVSGESREAAQPLL
                     SPLYYVQRALSPYADVVAPSEGNVANAIHDLVAQKVSTIVLADIGKLVAGTQEELESW
                     LKAGGVLIRFAGPRLEQGGDELLPVALRRGGRSLGGSLSWSTPQPLAPFEDNSPFRGL
                     AVPEDVRVNRQVLADPSVASEAEVWARLADGTPLVTAAKQGQGFIVLFHVTANSDWSN
                     LPLSGLFVEMLRRAVTLGPSQVALEAGKEPQAEASPAARRTASTGALPPLQTLDGFGQ
                     LTTPPLRAIPIAPDKIDSTVPSPDHPPGYYGPSGAARALNLITAKTVLTPIGKVEGSS
                     TVSGYIMKKPMALEPWLYLAAVALFVADIFAVLVLSGGFRFRRRAAAASVTILAAVAL
                     LSSLPAPARAQEPAGETAPASADDFASKAALNTRLAYVVTGDPEIDRTSEEGLAGLSK
                     VLRARTALEPAEPMGVDIEKDELAFFPLLYWPVREDASPLSDATLAKVDAFMKQGGLI
                     VFDTRDHETGFSAAGAQGGALSRLIGQLDIPLLEPVPESHVLTKSFYLMRSFPGRWDG
                     GALWVEAEPVDEAERSERSRRTDGVSSIVITSNDLAGAWALDQGNRPIYPAVPGGEVQ
                     REMAFRAGVNIVMYALTGNYKADQVHVPALLERLGQ"
     gene            complement(7855..8808)
                     /locus_tag="VFY74_01600"
     CDS             complement(7855..8808)
                     /locus_tag="VFY74_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5930433.1"
                     /translation="MAVTREKSLQPFSGEEAEAHRLAARIPALLVESQRIAHTVAHGT
                     HGRRRAGPGETFWQFRHYDQNDSVTGIDWRRSASSDTLFVREREWEAAHTVWLWVDLS
                     PSMRFRSRLAKVSKESRAVVLALALAELLARGGERVGLMGGQPFTGRAAARRVAEILM
                     GDTSETSLPPNARLRRFSECLLFGDFLEPIAEMSRRFEEIAAQGVRGHLIEVVDPAEE
                     TLPYAGRTEFAASEGRDRMIAGRAESLREDYQERLKRHRDDLVEMTRRLGWSFLVHHT
                     DRPPEEAVLSLHSRLAGLESDYRYRPARQAAASERAADRQP"
     gene            complement(8816..9814)
                     /locus_tag="VFY74_01605"
     CDS             complement(8816..9814)
                     /locus_tag="VFY74_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5930434.1"
                     /translation="MTTLAQADSQLVDRIEEVGAKIGRVRETTASTIFGQERVIDLAL
                     ITLLSGGHALLIGVPGLAKTSLVETLGAVLGLENKRIQFTPDLMPSDIVGSEVLEESA
                     GGRRGFRFVEGPIFTQLLMADEINRASPKTQSALLQAMQEHHVTVAGKRHDVPRPFHV
                     LATQNPLEQEGTYPLPEAQLDRFLLQIDVSYPDAAAERRMLFATTGVEERALKTILSG
                     DELMVAQRLVRQLPVGDQVVDAILKLVRSARPGTGIDEELEDLIAWGPGPRASQALML
                     AVRAKAMIEGRLAPSVDDVIELAEPVLKHRMALTFTARAEGIDMSQMVARLVEPLG"
     gene            10050..10538
                     /locus_tag="VFY74_01610"
     CDS             10050..10538
                     /locus_tag="VFY74_01610"
                     /inference="COORDINATES: protein motif:HMM:NF044966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX5930435.1"
                     /translation="MPKLNPCGDIGLKIGHDGTWYYQGGVIGRKGLVKLFASVLRLED
                     DGRYYLVTPVEKVPIAVETMPFVAVEMIREGRGEGQTLSFRTNVDDTVAAGPEHPIGF
                     QAGAEQRFIPFIVVRDGLKARLSRAIYYELAALAVETSATEEPGVFSGGAFFPFPLGS
                     AG"
     gene            10623..10883
                     /locus_tag="VFY74_01615"
     CDS             10623..10883
                     /locus_tag="VFY74_01615"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEX5930436.1"
                     /translation="MLRIALIDILLFSLPFLIYGAYMVAVKGVAPDKLWQGAPIFWLL
                     AAGIGLLLVVMAMLIQFSGGKPGGTYHPPSLEDGVIKPGEIN"
     gene            10895..12151
                     /locus_tag="VFY74_01620"
     CDS             10895..12151
                     /locus_tag="VFY74_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX5930437.1"
                     /translation="MPKNALERSSAPPSLKDAPWLKRPETARIFAALSVPGVETRAVG
                     GAVRNSLLGLAVAEIDLATTARPEQVMTLALKAGLKAVPTGIEHGTVTVVANGVPFEV
                     TTLRRDVETFGRHATVAFTENWEEDARRRDFTLNALYAASDGTVFDPLGGYDDVVGAR
                     VRFIGDAEARIKEDYLRILRFFRFNAHYGNGPFDPEGLRACVKLRGGIARLSAERVAG
                     EVRRLLVAPQAFRAVEAMFDYGLLTHVLGGVPRLERFRRLVEIEGALGRKAEAMPRLA
                     ALAVFVSEDAGRLAARLHLSNAEQGVLALGAEDHAASGLPDEDAAKRLLYRLGPTAYP
                     AAALIAWADSSAAPGDPNWHAAVTLDERWQAPVFPLKGSDLAALGEVEGPALGALLRK
                     LEAEWMAEGFAADRDRLLIRAKQIAR"
     gene            complement(12155..12973)
                     /locus_tag="VFY74_01625"
     CDS             complement(12155..12973)
                     /locus_tag="VFY74_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5930438.1"
                     /translation="MTRAQRQGRIRVGIGGWSYAPWRGVFYPKGLKQADELAYAASHL
                     TSIEINATHYRLQSAKSFRAWEAAAPQGFVFSVKGPRLVTQQKTLAETGSFIKRFFGS
                     GLAELGPKLGPVLWQFAPFKKFDRDDFARFLEHLPRDLEGLKLRHVIEPRHASFRTPE
                     FIGLLRDNEMSAAYTDAEVWPAIADVTADVVYARLQRGDDRLAAAYKPEELDAWADRA
                     KIWAKGESPKDLAHVDARHKPGIKPRDVFVYFIHEGKLRAPAAAMALIERLGRR"
     gene            complement(12992..13285)
                     /locus_tag="VFY74_01630"
     CDS             complement(12992..13285)
                     /locus_tag="VFY74_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5930439.1"
                     /translation="MPTYLVLGQFTDQGIRNVKDTSKRADAAKELAKKLGAKVLDLYW
                     TIGRYDAVFIVEAPNDETITAFALSSGKLGNVRTETLRAFTQAEIDQILSGVV"
     gene            complement(13546..15183)
                     /gene="groL"
                     /locus_tag="VFY74_01635"
     CDS             complement(13546..15183)
                     /gene="groL"
                     /locus_tag="VFY74_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085294.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5930440.1"
                     /translation="MPAKEVKFAQDARERMLHGVDILANAVRVTLGPKGRNVVLEKSF
                     GAPRITKDGVTVAKEIELEDKFENMGAQMVREVASKTSDTAGDGTTTATLLAQAIVKE
                     GAKSVAAGANPMDLKRGVETAVNAVIGELKSKAKKVTSSEEIAQVGTISANGDQDIGR
                     IIAEAMQKVGNDGVITVEEAKALETELEVVEGMQFDRGYISPYFITDADKMRVELENP
                     YILINEKKLSNLQTILPLLEAVVQSGKPLLVIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLQDIAVLAGGQVISEDLGIKLENVTLDMLGRAKKIVITKDDTTIVD
                     GAGKKADIEARVAQIKQQIEDTTSDYDREKLQERLAKLAGGVAVIKVGGATEIEVKEK
                     KDRVDDALNATRAAVEEGILPGGGVALIRAQKALDKLDAANEDQKVGINIVRRALQAP
                     ARQIAENSGEDGAVVVGKIVDKSDYSFGFNAQTGEYGDLVKQGVIDPAKVVRTALQDA
                     ASIAGLLITTEAMVAEKPKKKAPAMPPGAGGMGDMDF"
     gene            complement(15315..15629)
                     /gene="groES"
                     /locus_tag="VFY74_01640"
     CDS             complement(15315..15629)
                     /gene="groES"
                     /locus_tag="VFY74_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5930441.1"
                     /translation="MKFRPLHDRVVVRRIEEDERTKGGIIIPDTAKEKPQQGEIVAVG
                     PGARDEKGQLQPLDVKAGDRVLFGKWSGTEVKIDGEELLIMKESDILGVLEGAVAKKK
                     AA"
     gene            15866..16132
                     /locus_tag="VFY74_01645"
     CDS             15866..16132
                     /locus_tag="VFY74_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5930442.1"
                     /translation="MDTAFLRQIEGFSLTTAEIIYRLPDYPRLLQSYIWQDYDMAPRF
                     PKLTDFLDFWDANLDGALYRIRVAHKRLIAPTEFRFVEGVLRLH"
     gene            16231..16722
                     /locus_tag="VFY74_01650"
     CDS             16231..16722
                     /locus_tag="VFY74_01650"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX5930443.1"
                     /translation="MWRWLAVFLLGGVLGAAFGVALGFFFFPFVFPPPEAREQLTQAE
                     ASKLVAKGEFIHANRSDPVHYGKGGVSVYENVVFLHEDFEVGPGPAFHVYLVPTTNVR
                     DAGQVTGTMFVDLGGLRAFKGSQKYPIPAGVDLTKFPSVVIWCEQFSVLISPADLNFQ
                     QGS"
     gene            complement(16726..17688)
                     /locus_tag="VFY74_01655"
     CDS             complement(16726..17688)
                     /locus_tag="VFY74_01655"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5930444.1"
                     /translation="MSFVTAIGPEIENARDAPNAPADRQALTLVKPYQGMVAWPTVLL
                     AAGIVAAFALVTTLAAKGMIPLWLGLILNTFILYADQTPLHEACHGNIAGKDSRWLWL
                     NHLIGYVCGAILLHEYKAFRYMHLAHHRDTNNPEIDPDHWVAVNNPFLVLWRCLTIVF
                     WYHQYYWKHIAFHAHIPGMRPLTLHIAAMHALFYGVALWLSVFGWWREVLMLWIFPHI
                     LASALIIYFFAHLTHKPHEVHERYRDTNVFWVKGKIMEPIVNGLYMFQNFHLIHHLFP
                     RVPFYLYPKAFRSLKPVLEKERAHIYEFGHEPQPLLSDAGTARG"
     gene            complement(17988..>18115)
                     /locus_tag="VFY74_01660"
     CDS             complement(17988..>18115)
                     /locus_tag="VFY74_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEX5930445.1"
                     /translation="VAYGPPPWTPDWYAYCSSRYQSFDPSTGYFMGYDGYPHLCR"
BASE COUNT         3088 a         5784 c         6034 g         3209 t
ORIGIN      
        1 ggggtgcgga agggctaggg cgcggccata atcgaactgg tgcggggcgc cgcgtccctc
       61 cctataatgg cgccatgcgc ttcatcgccg ccgcaatcgc ctgcgcgatc gtgctgttcg
      121 gctcggccgc cgcccttgcg cagatctcgc ccgcggaaaa accgggcgcc aagaccaagc
      181 cagccgcgca gaaaccggcc gaaaaaaaac ccgcagccca gaaggctgct ccgtcccagc
      241 cgcaagtggt gatgcctgac gccgagaaga tcgtgcttct gctccgcacc acgctcatga
      301 cgcttaacga cgcgctccag accggaaatt tcacggtgat gcgcgacatc agcgcgccgg
      361 gtttccgcga cgccaacacc gcggcccggc tctcgctatc cttctccgat ctcgcgtcga
      421 agggggtcga cctctcgccc accagcgtca tcgcaccgca attgaccgag ccccccggtc
      481 tcgaccagaa aaagggcttg ctgcgcctca agggctactt tccgggtcag ccggtgcaga
      541 tcaatttcga gatgctgtac caggcggtcg atggccgctg gcgcttgttc ggattgtcgg
      601 tgcagccggg accgccaacg ccgccgcccg caagtgcgag cgcgcccgcg cctggcgcgg
      661 cgacgcagca atagcgaaag aagccgtggc gtccatcccg gcaagatcga agccaagcct
      721 gcttggccgg ctccggccga accgtctgtt gaccgccatg ttccgtccga tctggcgcat
      781 gcggcgcggc acgacgctcg gcgcgcaagg ggcggtgatc gacgccgagg ggcgcgtgct
      841 gctcgtgcgc cacggctacc gccccggctg gtggttcccg ggcggcggcg tcgaatgggg
      901 cgagacgctc gtgagcgcgc tcggccgcga gctcgaagaa gaggtggggg tcaagctcac
      961 gggcgcgccg gagctgcacg gcatcttctc gaatttcgcg agcttccccg gcgaccatat
     1021 cgcggtgttc gtggtgcgcc actggacgcg ccgcgaggat taccgcaagc gcggcgagat
     1081 cgcggaggcg cgcatgttcg cctggcagga cctgcccgaa cgcgtcgatc ccggcacgcg
     1141 cgcgcggctc gccgagatct tcgaccaagc cccgatcgat gcgcgctggt gatttcctcc
     1201 tcgcgggtca gcagtcgact tcccctctcc ccttggggag agggctttcc gccggacgcg
     1261 cgcagcgcgg ccaatggcgg gaagggtgag gggttctctt cttcgaagcc gttttgcggc
     1321 ttaccccctc accacgagcg ccaaaacgcg ctcgtgtgct ctccccaacc tgcctgcgcg
     1381 aagccgaagc ggcttcgctt cggcgaaggc aggcgggaga gcggaaaaaa taggagcggg
     1441 aggaggacta gatgaattac tgcaaacctg cgagcttcct ggcgatcttg gccacgtggt
     1501 gaccctggaa gctcgcgagg ttcagctcct tctgcgaagg caggcgcgag ccgtcgggag
     1561 ccgcgatggt gcccgcgccg taaggcgagc cgcccttgac ctcgctgata tcgggaagat
     1621 cgggcgccga ataggggagg ccgaccacca ccatgccgtg atgcagcagc gtccaatgga
     1681 acgaaacgat ggtcgtctcg ttgccgccgc cggtcgaggt cgaggtgaag acgctgccca
     1741 ctttgccgat cagcgcgccg gagacccaga gcggtccggt ctggtcgaga aagttccgca
     1801 tctgcgaggt catgttgccg aagcgggtcg gcgtgccgaa aatgatgccg tcatactcgg
     1861 cgagttcttg cgggtcggcg atcgcggcgg gctgatccac cttcatgccg gcggcgcgca
     1921 tgatgtcttc cggcatcagt tcgggcacgc gcttgacgct gacatgcacg tcgtcaaccg
     1981 cgcgagcgcc atgcgcgacc gcataggcca gcgtctcggt gtgtccgtat gaggagtgat
     2041 agagaacgag caatcgggtt gtgtcggcca tgatcgtccc cgcttacctc atcacggggc
     2101 gccgctcaac tgccccgttg cgcataagcc taacagactc aaggaatatg acgagagcgg
     2161 ataaacgccc ggggagcggt ccctgtcctg cggcttaaga cgaacgtagc gctagaccgg
     2221 cgcgggcgcg cgtgatagac gagcgccgcc atgtggaccc ggatcggccc aaccacgctt
     2281 ccccgacgac gagggcgcgc gcttctcgcg cgaactgcgt agccctcgcc gccgacacct
     2341 cctcgcattt gttcaaggtg ccccgccgcg gttgagggtt tgtctctcaa tcacctctct
     2401 gggacctcga tctcatgtct accggaaaat tcgaaatccg ccacgaaacc gatggcgatg
     2461 cgcccgttct gtcgcggctc gcggccgagg ccttcgggcc tggccgcttt acccgcagcg
     2521 cctaccgtgt gcgcgagggg gtggcacccg tcgccgggct ctctctgtgc ggcgtgctcg
     2581 atgggcgttt ggtcggggga atccgattca ccgcgatcag gatcggcgac aaggagggcg
     2641 gcgtcctgct cggaccgctc atcgtcgatc ccacagctat gggcagggga ttcggcaagg
     2701 ccctcgtcga agaagggttg gcgcgggcgc gcgccgaacg cttctgcctc gtgctgctcg
     2761 tcggcgacat gccctattac ggacggttcg gcttcacgcc cgtcatgccg gggcggatca
     2821 ccttgccggg acccgtcgat ccggcgcggc tgcttggtct tgagcttgtg accggagcga
     2881 tggaaggcgc cgccggaggc gtgaagggct atgcgcgtta gtggccttcg cggaaccagg
     2941 tcgcggcgag gaatccgagc agaagtccaa gcgcgataaa tccggagaag agcggaaaca
     3001 gacgcacgcc cttgacgtga taggcgtcgc gcctgtgcag cccgagccaa tcgtcgccat
     3061 gcatgacctg gccgccgcgg agcatcacca gtcgcggcag tttggcggcc tccgcttcgt
     3121 cacgtccgag ccagaagacg ccgccgccgg tctcctcgag aatgggtgag agcacctcgc
     3181 ctgtcgcggt caccgcggaa agttcccgcg cattggcgtt gccgaccgtg accacgttgg
     3241 caagtgcgcc cgaggagatg cgatagacac cttgctcggt cgcattgacg gcctttcgcc
     3301 acaacccgcg ctcgcccttg tcgagcgtta cgacctcctc cttgccggag ggcgccgtca
     3361 ccgtgacctt ctcgacctcg tccttgatcg agcgccgttc gatggagatc gtctggccgc
     3421 ggctcgtcgc cttcagcgtc tcttcctcga ggtccggctc cttcatcagc caatgggcga
     3481 gccggcgcag gagatcggta tagggtccgc cgccgtcgta gccgcgcgcc cacagccaag
     3541 tatggtccga gagcaggagc gccacgcggc cttggccgcg ctcgccgatg acgaggagcg
     3601 gctttccttg cgcgcccttc atcagcactt cggcatcgcg cgggtcggcc tcgacgacgc
     3661 ggaaccaacg tccccaggac ggctcgtcgc cgccggcgcc cggcaagtcg ctggtgacgg
     3721 gatgcttctc gccaagctcc gaaagcttgg gcttgaacgg ctcctccacc acgcggcccg
     3781 acggcacagc agggagaacc tgaccgagcg gcgtccggaa caggctgagc ggggaggcgt
     3841 aatcgtcgcc ggccgagacc agcaccgcgc cgcccttctc gacgtaacgg gcgacattct
     3901 ccaggtagag cagggggagc acgccccgcc gctgatagcg gtcgaagatg atcaggtcga
     3961 actgatcgag cttttcctgg aacagctcgc gggtggggaa ggcgatcagc gagagctgat
     4021 tgatgggcgt gccgtcctgc ttctcgggcg gccgcagaat ggtgaagtga acgaggtcga
     4081 ccgcggcgtc ggacttcagc atgttgcgcc aggtgcgctc gccggcatgc ggctctccgg
     4141 agacgagcag cacccgcaga ttctcacgca ccccctctac cgtcagcacg gcgcgattgt
     4201 tcacggtcgt gatctcgcct tgcgcgggct cggcctcgat ctcgacgatg ttgggaccgg
     4261 catggttgat atcgaccgga atcgcgacct tgtcgccggc gcggacgctc tgggtcacgg
     4321 gcggctggcc ctgatgggtg accgtgagct tgacggctcc gccgctcccg gccgccgcgg
     4381 tatcggtgac tttgacctcg atgtcctggc tgctgccgac aatgccgaat cgcggcgcgc
     4441 tcagaacctg aagcctgcgg tcgaattcgc cttgcttgcc ggtcagaagc gcatgcaccg
     4501 gcgcgtcgaa accgagcccc gcggcggcgg ccggcacgtc atgcacctcg ccgtccgtaa
     4561 tcatgagcac gccggcgagc cggtcgggcg gcacatcggc aagcgcctga gcgagatcgg
     4621 tgaacagcat ggtgccgtcg cgctcgctgt cggccgcggt cgaggtcgag cgcacccagc
     4681 gcgtgtcgag attggaaaac gccttgagcc gttcctccaa gtccctgcgg acctgctcgg
     4741 tgcgcgcggt gcggccggcg atcgcctggc tctggctttc gtcaacgacg acggcaacca
     4801 cgttgttgag cggctcgcgc tcttcctgtt tcaagtgcgg attggcgatg gcgaggagca
     4861 gcagcgtata gctcacgagg cggaaaatcg cgccgcggcg cgagcgccag aacaggagcg
     4921 cgagcagaac cgcgccgagc gccgcaagtc cccacagaac cggccagggc acaaagggta
     4981 tgaactcgat cgaccagctc atggcctact gtcccaggcg ctcgagcagg gcgggcacgt
     5041 gcacctggtc ggccttgtag ttgccggtga gcgcgtacat cacgatgttg acaccggcgc
     5101 ggaacgccat ctcgcgctga acttcgccgc cgggcactgc cgggtagatg ggccggttgc
     5161 cctggtcgag cgcccaggcg ccggccaagt cgttcgaggt gatgacgatc gagctcacgc
     5221 catcggtgcg gcgcgaacgc tcgctgcgct cggcctcgtc gacgggctcg gcctcgaccc
     5281 agagtgcgcc gccgtcccaa cggccgggaa agctccgcat caggtagaac gacttggtaa
     5341 gcacgtgact ctcgggcacc ggctcgagca gcggaatgtc gagctggcca atcaggcgac
     5401 tgagcgcccc gccctgcgcg cccgccgcgg agaagcccgt ttcatggtcg cgggtgtcga
     5461 agacgatgag accgccctgc ttcatgaagg catcgacctt ggcgagcgtc gcgtcggaga
     5521 gcgggctcgc atcctcgcgc accggccaat agagaagcgg gaagaaggcg agctcgtcct
     5581 tttcgatatc gacgcccatg ggttcggcgg gctcgagcgc ggtgcgggcg cgcaagacct
     5641 tgctcaatcc ggccaagccc tcttcgctcg tgcggtcgat ctcggggtcc ccggtgacga
     5701 cataggcgag gcgcgtgttg agggcagcct tgctcgcgaa gtcgtccgcc gaggcgggcg
     5761 ccgtttcgcc cgcgggctcc tgcgcgcgag caggcgcggg gagcgaggac agaagtgcca
     5821 cggcagcgag aatggtcacg ctcgcggcgg cggcgcgcct gcggaaacga aaaccgccgc
     5881 tcagcacgag cacggcgaaa atgtcggcga caaaaagggc tacggcggca agatagagcc
     5941 aaggctccag cgccatcggc ttcttcatga tgtagccgct caccgtgctc gacccttcga
     6001 ccttgccgat cggcgtcaga acggtcttgg cggtgatgag gttgagcgcg cgcgcggcac
     6061 ccgagggacc gtaatatccg ggcggatgat cggggctcgg caccgtgctg tcgatcttat
     6121 ccggcgcgat cggtatcgcg cggagtggcg gcgtcgtcag ctggccgaag ccgtctaggg
     6181 tctggagcgg cggaagcgcg ccggtcgagg cggtgcggcg agcggcgggt gaagcctctg
     6241 cctgaggctc tttgcccgcc tcgagcgcga cctggctcgg accgagcgtc accgcgcggc
     6301 gcaacatctc gacgaacaat cccgagagcg gcaagttcga ccagtcggaa ttcgcggtga
     6361 cgtggaacag gacgataaag ccctgaccct gcttcgcggc cgtcacgagc ggcgtgccgt
     6421 cggcaagccg cgcccagacc tccgcctcgc tcgccacgct tggatcggcg agcacctgcc
     6481 ggttgaccct gacatcctcg ggcacggcaa gaccgcggaa gggactgttg tcctcgaacg
     6541 gcgcaagcgg ctgcggcgtg ctccaggaca gcgagccgcc gagcgagcgg ccgccgcggc
     6601 ggagcgcgac cgggagcaat tcgtcgccgc cttgctcgag ccttggtccg gcgaaacgga
     6661 tgaggacgcc tcctgccttg agccaggact ccaactcttc ttgggtgccg gcgacgagct
     6721 tgccgatatc ggccagaacg atggtcgaga ccttctgcgc gaccaggtcg tgaatggcgt
     6781 tggcgacgtt gccctcgctc ggcgccacga cgtcggcgta gggcgacagc gcgcgctgca
     6841 cgtaatagag aggcgaaagc agtggctgcg cggcttcccg cgattcgccc gagacgatgc
     6901 cgacgcgatg ccattgcgag cgggcgtcga ggagagagac cgcgcccgcg gaacgttcgc
     6961 ctgcgatggc gatgcgggcg acctggttgc ggatctcgag cggcaagtcg aattgaaccg
     7021 tagtctcacg cgcgccctga ccgatggtga atgccgtttc gccgagaggc tcgccgcggc
     7081 cggtgagggc cttgatgacg ccggagcgcg gtccttcggc gccgctcaag atgcgggcgg
     7141 caaacgcacc gctctcggcg gcgccctgtc cgagcgcaag cgccgcgtca ccgcgctcgg
     7201 gccggagcac ggaaaggctg ccgccatcgc cggcaagctt ggcaagagcc tcgctgaagg
     7261 tcttgccatc gccatagtcc aagccatcgg aaagccacag cacgctgaat ccggtcttcc
     7321 cgccgagttc tttttcaagc gtcgcggcaa gggcggcgcg agcgggagca taaggctcgg
     7381 gattgatgcc gccgatgcgc tcgcgcgcct tatcggggct caagggagcg ctgaccggtg
     7441 gaccgggcgc cgacggggcg agcacgatgg tgcggccgtc gcgcgccgcc cggtcgatcg
     7501 ccgcctcgat tgcctcgcgg cgttcgggcc aatgcgcgcc cgaggcccag ccattgtcga
     7561 tcacgatgag gagagggccc gaaccggtga agttttggcg gtcggggttg agcacgggcc
     7621 gtgccagcgc gaggatgatc aaggcggcga gaagcatgcg aagcgcggtc agccaccatg
     7681 ggctccgcgc cggcgtctcc tccgtggtct tgagatcctt gaggagtcgc gtcggcggaa
     7741 agaccacaag ctgcgggctc ggcggggtga gccgcaacag ccaccagatt gcgggaagcg
     7801 cggcgagagc cgccaatacc caaggctgaa ggaatccgat tgcgccgatg gtgatcatgg
     7861 ctgccggtcc gcggctctct cgctggctgc ggcctgccgg gccggccggt agcgatagtc
     7921 gctctcgagc ccggcgagac ggctatggag cgacagcacc gcctcctccg gcggacggtc
     7981 ggtgtgatgg acaaggaacg accagccgag ccggcgcgtc atctcgacaa ggtcgtcgcg
     8041 atggcgcttg agccgctcct ggtagtcctc gcgcaagctc tcggcgcggc cggcgatcat
     8101 ccggtcgcgg ccttcgctcg cggcgaactc ggtgcggccg gcgtagggaa gcgtttcctc
     8161 tgccggatcg acaacttcga tcagatggcc gcgcacgcct tgcgcggcga tctcctcgaa
     8221 gcgcctgctc atctcggcga tcggctcgag gaagtcgccg aacaggaggc actccgagaa
     8281 acggcgtagt ctcgcgttcg gcggcagact tgtctcgctg gtatcgccca tgaggatctc
     8341 ggcaacgcgg cgggcggcgg cgcgtcctgt gaagggttgt ccgcccatga ggccgacgcg
     8401 ctcgccgccg cgggcgagaa gctcggcaag agcaagcgcc agcacgacgg cgcggctctc
     8461 cttggacact ttggcaagcc gggagcggaa ccgcatggag ggcgagagat cgacccagag
     8521 ccagaccgta tgcgccgcct cccactcgcg ctcgcgcacg aagagcgtgt cggagcttgc
     8581 cgaacggcgc cagtcgattc cggtgaccga gtcgttctgg tcgtaatgac ggaactgcca
     8641 gaaggtttcg ccgggcccgg cgcggcggcg gccatgcgta ccgtgggcga cggtgtgggc
     8701 aatccgctgc gactcgacga ggagcgcggg gatgcgggcc gccaagcgat gggcctcggc
     8761 ttcctcgccg gaaaatggct gaagcgattt ctcccgagtg acggccaagc tcagatcacc
     8821 ctagcggctc gacaagcctc gccaccatct ggctcatgtc gatcccttcg gcacgggcgg
     8881 tgaaggtgag cgccatccgg tgcttgagca caggctcggc aagctcgatc acgtcatcga
     8941 cggaaggcgc gagccgtcct tcgatcatgg ccttggcccg cacggcgagc atcaatgcct
     9001 ggctcgcgcg ggggccgggg ccccaggcga tcaggtcctc gagctcctcg tcgatgccgg
     9061 tgccaggccg cgccgaacgc acgagcttca ggatcgcatc caccacctgg tcgccgaccg
     9121 gcagctggcg cacgagcctc tgcgccacca tcaactcgtc gcccgacagg atcgtcttga
     9181 gcgcgcgctc ctcgacgcca gtcgtggcaa acagcatgcg gcgctcggcg gcggcgtccg
     9241 gataggacac gtcgatctgc aacaggaagc ggtcgagctg ggcttcgggc aggggatagg
     9301 tgccttcctg ctcgagcggg ttctgggtcg cgagcacatg gaagggacgg ggcacgtcgt
     9361 ggcgtttgcc cgccacggtg acatgatgct cctgcatcgc ctggagcagc gccgactgcg
     9421 tcttggggct cgcgcggttg atctcgtcgg ccatgagcaa ctgggtgaag atggggcctt
     9481 cgacgaaacg aaaaccgcgg cgcccgcccg cgctctcctc gagcacctcg gagccgacga
     9541 tgtcggaggg catcaggtcg ggcgtgaact ggatgcgctt gttctcgagg ccgagcacgg
     9601 cgccgagcgt ctcgaccagg ctcgtcttgg cgaggcccgg cacgccgatg agcagcgcgt
     9661 gaccgccgga gagcagcgtg atcaaggcaa ggtcgatgac gcgctcctgg ccgaaaatcg
     9721 tcgaggccgt ggtctcgcgg acgcgtccaa tctttgcacc aacctcctcg atacgatcga
     9781 cgagctggga atcggcttgg gcaagggttg tcatggcttc agtttcactc gatcctgatg
     9841 gtagggaata tagggccaaa ggcctcgcgg agtagaagca ctttgagcgg caattcggtc
     9901 acaatcgtgt tgattccggc ctccagactg atccggcatg ccggtgagcc gacaatttgg
     9961 tcagaagcga cctagcgatg tcccgccaag atcaaaggga cgctgtcgta gcaccgggtc
    10021 aggcccgggg caaacaggct tcagaaggat tgccaaagct taatccttgc ggggatatcg
    10081 ggctcaagat cggccatgac gggacctggt attaccaggg cggcgtcatt gggcggaagg
    10141 ggctggtcaa gctgttcgcc tctgtgcttc gcctcgagga cgatgggcgg tactacctgg
    10201 tcacgccggt cgagaaggtg ccgatcgcag tagagacgat gcccttcgtc gcggtggaga
    10261 tgatccggga agggcgcggg gaggggcaaa ccctctcatt ccgcacaaac gtggacgaca
    10321 cggtcgcggc cggacccgag cacccaatcg gctttcaggc cggagcggag cagcgcttca
    10381 tccccttcat tgtcgtgcgc gacggtttga aagcgcggct ctcccgcgcg atctattacg
    10441 agcttgcggc gctcgcggtc gagacctccg cgaccgagga gccgggtgtc ttcagcggcg
    10501 gggcgttctt cccgtttccg ctgggaagcg ctggctagag catcgccgca gcactcgatt
    10561 tgcgggacga gcgccgctat ggtgctgctc cggtttttcg gcatgaggtc cctggctctt
    10621 cgatgttgcg catcgcgctg atcgacattc tcctcttctc gctgcccttt ctgatctacg
    10681 gggcctacat ggttgcggtg aagggcgtgg cgcccgacaa gctttggcag ggcgccccga
    10741 tcttctggct tctggcggcg ggcatagggc tcctgctcgt cgtcatggcg atgctgatcc
    10801 aattctcggg cggcaagccc ggcgggacct atcacccacc gagcctcgag gacggcgtca
    10861 tcaagcccgg cgagatcaac tgacggcggc gcccatgccc aagaatgcgc tcgagcgctc
    10921 ctccgcgcct ccttcactca aggacgcccc gtggctgaaa cggcctgaga ccgcgcgcat
    10981 cttcgccgca ctctcggtgc cgggcgtgga gacccgcgcg gtgggaggcg ccgtgcgcaa
    11041 cagcttgctc gggcttgccg tggcggagat cgacctcgct accaccgctc gccctgaaca
    11101 ggtgatgacg ctcgcgctga aggcggggct caaggccgtt ccgactggaa tcgagcatgg
    11161 gacagttacc gtcgtcgcca acggcgttcc cttcgaggtg acgacgctac ggcgcgacgt
    11221 cgagacgttc ggccggcatg cgacggtcgc atttaccgag aattgggaag aggacgcgcg
    11281 ccggcgcgac ttcacgctta acgcgcttta cgcggcgagc gacggaaccg tgtttgatcc
    11341 gctcgggggc tatgacgatg tcgtcggagc tcgcgtgcgg ttcattggcg atgcggaagc
    11401 gcgcattaaa gaggactact tacgcatcct gcgcttcttc cgctttaacg cccattacgg
    11461 caacgggccc ttcgatccgg aggggcttcg ggcgtgcgtc aagctccgcg gcgggatcgc
    11521 tcggctttcg gctgagcggg tcgcgggcga agtcaggcga cttctcgtcg cgccgcaggc
    11581 gttccgggcg gtcgaggcga tgttcgatta cggtcttctg acgcatgtgc ttggcggcgt
    11641 gccgcgactc gagcggttca ggcgcttggt cgaaatcgaa ggcgcgctcg ggcgtaaggc
    11701 cgaggccatg ccgaggctcg cggcgcttgc cgtgttcgtc agcgaggacg ccggccgcct
    11761 ggccgcgcgc ttgcacctct cgaatgcgga gcagggcgtg cttgcgctcg gggcggaaga
    11821 ccatgccgca agcggtctgc cggatgagga cgcagccaag cgtctgcttt accgcctggg
    11881 gcctaccgct tatccggccg ccgcgttgat cgcctgggcc gattcgagcg cggctcctgg
    11941 cgacccgaac tggcatgccg cagttacgct tgacgaacgc tggcaggcgc cggtctttcc
    12001 gctcaaggga tcggacctcg ccgcgctcgg cgaggtggag ggccccgcac tcggcgcgct
    12061 gctgcgcaag ctcgaggcag aatggatggc agaaggcttc gccgccgacc gcgaccggct
    12121 cttgatcaga gcgaagcaga ttgcgcgctg atcatcagcg ccggccaagc cgttcgatca
    12181 gcgccatggc cgcggccgga gcgcgcagct tcccctcatg gatgaaataa acgaaaacat
    12241 cgcggggctt gatgccgggc ttatgcctgg cgtcgacgtg ggcgagatcc ttaggcgatt
    12301 cgcccttcgc ccatatcttc gcacgatccg cccaggcatc gagctcttcc ggcttgtagg
    12361 cggccgctag ccgatcgtcg ccgcgctgca gccgggcata gaccacgtcg gcggtcacgt
    12421 cggcgatcgc gggccagact tcggcgtcgg tgtaggcggc gctcatttca ttgtcgcgca
    12481 ggaggccgat gaattccggc gtccggaaac ttgcatggcg tggctcgatc acatgacgga
    12541 gcttcagccc ttcgaggtcg cgcggcaaat gctcgaggaa gcgggcgaag tcgtcgcgat
    12601 cgaatttctt gaagggcgca aattgccaaa gcacaggccc gagcttgggc ccgagctcgg
    12661 caaggccgga gccgaagaag cgcttgatga agctgccggt ctcggcgagc gttttctgtt
    12721 gggtgacgag acgagggccc ttcaccgaaa agacgaagcc ctgcggcgcg gcagcctccc
    12781 acgcgcggaa gctcttcgca ctttgcagcc tatagtgggt cgcgttgatc tcgatcgagg
    12841 ttaggtgcga ggctgcgtag gcgagctcgt ccgcctgctt caggcccttc ggatagaaca
    12901 caccgcgcca cggcgcgtag ctccagccgc cgatcccgac gcgaatgcgc ccttgccgct
    12961 gtgctctggt catcaaggcc gaacgctggc gttaaaccac tccactcaga atctgatcga
    13021 tctctgcttg cgtgaacgcc cgcaaggtct cggtccgaac attgccgagc ttgcccgagc
    13081 tcaacgcgaa ggcagttatg gtctcgtcgt ttggcgcttc gacgatgaag acggcgtcat
    13141 accggccgat ggtccaatag aggtcgagca ccttcgcgcc gagcttcttt gccagctcct
    13201 tcgcggcatc ggcgcgcttg ctcgtgtcct tcacgttgcg gatgccctgg tcggtgaatt
    13261 gaccaagaac aagataggtt ggcatggttg tctcccggct cgatttgcgc tccacggccg
    13321 aggctaagag attgacccag tttgcgccag acggaggctt ctactcgtcc agccacgtgc
    13381 ggtccttctg atgttcgccg ccatctggaa tgagagccct gccggtcaac ccggcggcca
    13441 agggcgcgca aaaagccgga tgaggataac cgcaaaagaa gagccgcgcc tgatgaggcg
    13501 cggctcgact tacgcgaatc gttacagacg cttacgccac aggacttaga agtccatgtc
    13561 gcccatgccg ccggcgcccg gaggcatcgc gggcgccttc ttctttggct tctcggccac
    13621 catggcttcg gtggtgatga ggagaccggc gatggaggcg gcatcttgga gcgcggtgcg
    13681 cacgaccttg gcggggtcga tcacgccctg cttgacgaga tcgccatatt cgccggtttg
    13741 cgcattgaac ccgaaggaat agtcgctttt atcgacgatc ttgccgacaa ccacggcgcc
    13801 gtcttcgcct gagttctcgg cgatctggcg agccggagct tgcaacgcgc gccggacgat
    13861 attgatgccg accttctggt cctcgttggc cgcatcgagc ttgtcgagcg ccttctgcgc
    13921 gcggatcagc gccacgccgc cgcccggcag gatgccttcc tcgaccgccg cgcgggtcgc
    13981 gttcaaggcg tcgtcgacgc gatccttctt ctccttgacc tcgatctcgg tggcgccgcc
    14041 gaccttgatc accgcaacgc ccccggcgag cttggccagc cgctcctgca gcttctcgcg
    14101 gtcgtagtct gacgtcgtgt cctcgatctg ctgcttgatc tgcgcaaccc gcgcctcgat
    14161 gtcggctttc ttgccggcgc catcgacgat cgtcgtgtca tccttggtga tgacgatctt
    14221 cttggcgcgg ccgagcatgt cgagggtcac gttctcgagc ttgatgccga gatcctccga
    14281 aatcacctgg ccgccggcga gcacggcgat gtcctggagc atggccttgc ggcggtcccc
    14341 gaagccggga gcctttacag ccgcgacctt caagccgccg cggagcttgt tgaccacaag
    14401 ggtcgcgagc gcctcgccct cgacgtcttc ggcgatcacg agaagcggct tgcccgattg
    14461 caccacggcc tcgagcagcg gcaggatggt ctgaaggttg gagagcttct tctcgttgat
    14521 gaggatatag gggttttcaa gctcgacgcg catcttgtcg gcgtcggtga tgaaataggg
    14581 ggagatgtag ccgcggtcga actgcatgcc ttcgaccact tccagctcgg tctcgagcgc
    14641 cttggcctcc tccaccgtga tcacgccgtc attgccgact ttctgcatgg cttcggcgat
    14701 gatgcggcca atgtcctgat cgccgttggc ggagatggtc ccgacttggg caatctcttc
    14761 gctggaggtg actttcttcg ccttcgactt cagctcgccg atgaccgcat tgacggccgt
    14821 ttcgacgccg cgcttgagat ccatgggatt ggcgccggcg gcaaccgatt tcgcgccctc
    14881 cttgacgatg gcctgagcga gcagggtcgc ggtcgtagtg ccgtcgccgg cggtgtccga
    14941 ggtcttcgaa gcgacctcgc gcaccatctg cgcgcccatg ttctcgaact tgtcctcaag
    15001 ctcgatctcc ttggcgacgg tcacgccgtc cttggtgatg cgcggcgcgc cgaacgattt
    15061 ttcgagcacg acgttgcggc ccttggggcc gagcgtcacc ctgacggcat tggccagaat
    15121 atcgacgcca tgcagcatgc gctcgcgggc gtcttgggca aatttcactt ccttcgctgg
    15181 cattgatctt actccgttgg ctcaaccgcc gttctcggcg ctgtcgcgaa aaacgcggac
    15241 ggcagcggag atggcgtcga aatcaaggtc atcctcaaaa gctctgcgga gccgtcccgg
    15301 gatgacgaat gtccttaggc agccttcttc ttcgcgaccg cgccttcgag cacgccgagg
    15361 atgtcgctct ccttcatgat gagaagctcc tcgccgtcga tcttgacctc ggtgcccgac
    15421 cacttgccga acagcacgcg gtcgccggcc ttcacgtcga gcggctgaag ctggcccttc
    15481 tcgtcacggg cgcctgggcc tacggcaacg atctcgccct gctgcggttt ctccttcgcg
    15541 gtgtcgggga tgatgatccc gcctttggtc cgttcatcct cctcgatacg gcgcacgacg
    15601 acgcggtcat gcaatggacg aaacttcatc ttggctcctc ctccgggcaa tgttgattga
    15661 cggggcgttc gcaagaaaac ccaccgccgc gctatttaaa ttgaggcatt agcactttca
    15721 agaggagagt gccaggaccg ctagcaatcc gacctgcttg ctgccaagat gctgatttga
    15781 tttggaaaaa ttctcaacag ggctgggatc ggcggtaaac ttttggcaaa atgaaaggaa
    15841 tcaatatagc ggggacacag cctccatgga caccgcgttt cttcgccaga tcgagggctt
    15901 ctcgctgaca acggcggaaa tcatctaccg cctgcccgac tatccgaggc tgctgcaaag
    15961 ctacatctgg caggactacg acatggcccc gcgcttcccc aagctcaccg actttctcga
    16021 tttctgggac gccaatctcg acggcgcgct ctaccgcatt cgcgtggcgc acaagaggct
    16081 gatcgcaccg actgaattcc gcttcgtcga aggcgtgctc aggctgcact aatacgagac
    16141 ggcaagggcg acgttcgcac tcgacctttt atgcggatct cgctcaagcg cgttttccat
    16201 gccaagctgc gatgctcgtg aggtgtcgcc atgtggcgat ggttggcggt ttttcttctg
    16261 gggggcgtgc tcggcgcggc cttcggcgtc gcgctcggct tctttttctt tcccttcgtc
    16321 tttccgccgc ccgaggcgag ggagcaactg acacaggccg aggcgagcaa gctcgtcgcc
    16381 aagggcgagt tcattcacgc caatcggagc gacccggtcc attacggaaa aggcggggtc
    16441 agcgtctatg agaacgtcgt gttcctgcac gaggatttcg aagtcggtcc aggccctgca
    16501 ttccacgtct atctcgtgcc gaccaccaat gtcagagacg cggggcaagt gaccggaacg
    16561 atgttcgtcg atctcggcgg cttgcgcgcc ttcaagggga gccagaaata tccgatcccc
    16621 gccggcgtcg acctcaccaa attcccgagc gtcgtgatct ggtgcgagca gttctcggtg
    16681 ctgatttctc ccgccgacct caacttccag cagggttcct gaacgctatc cccgggctgt
    16741 gcccgcgtcg ctcaagaggg gctgtggctc gtgcccgaac tcgtagatgt gggcgcgttc
    16801 tttctcgagg accggcttca gcgagcggaa ggccttcgga tagaggtaga agggcacgcg
    16861 cgggaacagg tggtggatca ggtggaagtt ctggaacatg taaagcccgt tcacgatcgg
    16921 ctccatgatc ttgcccttca cccagaacac gttggtgtcg cgataacgtt catgcacctc
    16981 atgcggcttg tgggtgaggt gagcgaagaa atagatgatc agcgccgagg cgaggatgtg
    17041 ggggaagatc cacagcatca gcacttcgcg ccaccacccg aataccgata gccaaagcgc
    17101 gaccccgtag aagagcgcgt gcatcgccgc gatatgcagc gtgagcggcc gcattcccgg
    17161 gatatgcgcg tggaaggcga tgtgcttcca gtaatactga tggtaccaga acacgatggt
    17221 gaggcagcgc cacagcacga ggaaggggtt gttcaccgcg acccaatgat cgggatcgat
    17281 ctccggattg ttggtgtcgc gatggtgcgc gagatgcatg tagcgaaacg ccttgtactc
    17341 atgcagcaga atcgcgccgc atacatagcc gatgagatgg ttgagccaga gccaccgcga
    17401 atccttgccg gcgatgttgc cgtggcaggc ctcgtgcagc ggcgtctgat cggcgtagag
    17461 aatgaacgtg ttgaggatca gcccaagcca gagcgggatc atgcctttcg cggcgagtgt
    17521 cgtgaccaag gcgaaagccg cgacgatacc ggccgcgagc agcaccgtcg gccacgccac
    17581 catgccctga taaggtttga cgagcgtcag cgcctgccgg tcggcgggag cgttcggtgc
    17641 gtcgcgcgcg ttctcaatct cgggtccgat cgccgtgacg aagctcatgg tccagtcctc
    17701 ctgcccccaa cggcgtcgtc ggcccgtcac atttgggccc cgcgcttctc tttggaatca
    17761 tctcaaaccc gcagaatttt gcgccgcatt gcggccacat tcaagacaca atggggacgg
    17821 gacagcgcac gaatgacggc acggtaaacg gctcgtcaaa aaaacttgcg cctggctgag
    17881 gcagctcaac gtcaggtgga gcgcggggca gacgtacgtt cttggaccag gaccaagagc
    17941 cggcggcgcc ggggcgaagc cgggcgcgct acgcgagtcg gggtcagcta ccggcacaaa
    18001 tgagggtagc cgtcatagcc catgaaatag ccggtgctgg gatcgaagct ctggtagcgc
    18061 gatgagcaat aggcatacca gtccggcgtc catggcggcg ggccatacgc aacgg
//
LOCUS       DASUQP010000033         5664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1786727, whole genome shotgun sequence.
ACCESSION   DASUQP010000033 DASUQP010000000
VERSION     DASUQP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786727"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1086
                     /gene="pip"
                     /locus_tag="VFY74_01665"
     CDS             109..1086
                     /gene="pip"
                     /locus_tag="VFY74_01665"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX5930446.1"
                     /translation="MNEQLIPKEKRLTLYPELVPHRTGRLRVSELHELYFEECGSPAG
                     KPALLLHGGPGGGINPIMRRYHDPHAYRMVLYDQRGCGRSTPHASLEENTTWHLVDDI
                     ERLREHLGIERWQVFGGSWGSTLALAYAETYPERVTELVLRGIFTLRRAELEWFYQEG
                     CSWIFPEDFEHYAGVIPESERDDMIGAYYRRLTDDDSDVQIEAARTWSAWEGKTLSLL
                     PDPDRVRRFSEDHYALAFARIECHYFINGGFFHADDQLIADAHRLRSIPGVIVHGRYD
                     VVTPVKIAYDLARAWPEADLRIVADAGHAMTEAGIVHELVAATDRFAET"
     gene            1110..1656
                     /locus_tag="VFY74_01670"
                     /pseudo
     CDS             1110..1656
                     /locus_tag="VFY74_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115806.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            complement(1679..2113)
                     /locus_tag="VFY74_01675"
     CDS             complement(1679..2113)
                     /locus_tag="VFY74_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5930447.1"
                     /translation="MTEMMQASHSLSVATQGKGFIDITGALASWLKQIGGANGVLTVF
                     VRHTSASLTIQENADPNVHRDLTDALEALAPEGADYAHQEEGPDDMPSHIKSMLTNVS
                     LGIPVVDGKMALGTWQGVYLIEHRASPHRRTVALSFIGEVRG"
     gene            2373..4346
                     /locus_tag="VFY74_01680"
     CDS             2373..4346
                     /locus_tag="VFY74_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930448.1"
                     /translation="MSSQASWQTGGAGWGEGFPDEQSVEGLLRHLLVQVEETERRYSD
                     ALDELQGRLDQLALKTDAARSGATGGGQALDRLHDQVSGLARRFEREASSSLDDFERL
                     GRAVMGGLDRDASGFSFDPSAPPYTSPTPEPNSVFSSFPPLPEADRDLSKRLIEMAHR
                     LEHSVDAAMTPRSLDDLPARIDAVGRQIADALAALPKPVSLEPLEHQIAYVAHKIGRA
                     ETELAKIGDIETALHRLIERMDRQAGQLGDVAAKAATEAARLVSGEARLDAATAERLD
                     TMHRDLMAMNARSSAADDRLAGSIEAVHQSLKQLVLETERSASRATALSPHAPFPERM
                     PDAHGEQAESVSAKGVEYQMERRAPPGVEAKPLEAKLPEASPLEARPLEARSLEARPP
                     FGRAKRAGSGDTLVDLDEERSVDLDVPLGSRSLVKRVARTESEMENDLVAAARRAAQA
                     AARRAAERTGRDMRNWAPAGARTSSRVETLRAETPRGAETHRSEALSRRSRPLLIVAA
                     AVLLVLSAILLYSRLQTKSWPEFLAPAAEESAPLPSAPIEESPLPGAGDKVPDAEPEK
                     ETAPAPGPVPPDAAANPPRDAAGGTGKSANVTDIAKSTYWPATVVEEPVEAAPATGVQ
                     ATRQAEPAALRAAEEPLLPPGVVFTVEDPSRSF"
     gene            4565..4897
                     /locus_tag="VFY74_01685"
     CDS             4565..4897
                     /locus_tag="VFY74_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930449.1"
                     /translation="MTKLDQLNAEKGKKFGEICAFMARELGYGDFARIGPEDRKYIEQ
                     EAEQQLSDWEETTEFRLRPDIRPSTPLRRLLSEHQHICERILDEQDIEIGLWAYKKRK
                     TGRGRVSL"
     gene            5405..>5664
                     /locus_tag="VFY74_01690"
     CDS             5405..>5664
                     /locus_tag="VFY74_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930450.1"
                     /translation="MTIAPDPGAYDSAVVTDFLLFGATLAAVAIFHRHTLAAALIGLG
                     AIIIKKLLGEGFAEGAGLSGLLPISHMNGCCLRTCFCFWSASP"
BASE COUNT         1011 a         1865 c         1783 g         1005 t
ORIGIN      
        1 gcggcgagac gatctacgat cgcgcgcaat tgcgcgcagc ggcagcggcg gctgccgctg
       61 cggcgaccgc cgagcaagag ccgtgatctc gccgaaagac gaaacaagat gaacgagcag
      121 ctgatcccca aagagaagcg gctcacgctc tatcccgagc tcgttcccca ccgcaccggg
      181 cggctcagag tctcggagct gcatgaactc tatttcgagg aatgcgggtc gccggcgggg
      241 aagcccgcgc tgttgctgca tggcggcccc ggcggcggca tcaaccccat catgcgccgc
      301 tatcacgacc cgcatgcgta tcgcatggtc ctctatgatc agcggggatg cgggcgctcc
      361 acgccgcatg cctcactcga ggaaaacacc acttggcacc tcgtcgacga catcgagcgg
      421 ctccgagagc atctcggcat cgagcgctgg caggtgtttg gcggttcatg gggttcgaca
      481 ctcgcgctcg cttatgccga gacgtatcct gagcgcgtga ccgagcttgt actcaggggc
      541 atcttcacct tgcgccgcgc ggagctcgaa tggttctacc aggaaggctg cagctggatc
      601 ttccccgaag acttcgagca ctatgccggc gtcattcccg agagcgagcg ggacgacatg
      661 atcggcgcgt attaccggcg cctcacagat gacgactccg acgtgcagat cgaagcggcg
      721 cggacgtgga gcgcctggga gggcaagacg ctctcgctcc tgcccgaccc cgatcgggtg
      781 cgccgcttct ccgaggacca ttacgcgctc gcctttgcgc gcatcgagtg ccattacttc
      841 atcaatggcg gtttcttcca cgccgacgat caactgatcg ccgacgcgca tcgtcttcgt
      901 tcgatcccag gcgtgatcgt tcacggccgt tacgacgtgg tgacgccggt caagattgct
      961 tacgacctcg cgcgcgcatg gcccgaagcg gatttgcgca tcgtcgccga tgccggtcat
     1021 gccatgaccg aggccggcat cgtgcatgag ctggtcgccg ccaccgaccg cttcgcggaa
     1081 acataatgcg cgataggaat gtcgcggcga tgccggacct caacaccttg cccagtcatt
     1141 tgcccaggcc cgtcgatgac ggcgcctgcg ctcacctcac cggcatgacg atgcctgcga
     1201 tcacgctgca agcgacccaa ggggcgcccg tcaatctcgg cgcgcttccg ccgggaagat
     1261 tgtgatttat tgctacccgc gacccgggac accgggcgag gccctaccgg aaagttggga
     1321 tgaaatcccg ggcgcgcggg gttgtgcgcc ttccgcgacc accatcgtga ccttgccgcg
     1381 ctcggagcgc acgtctttgg gctaagcacg caacccacgg attatcagcg tgagatggcg
     1441 gagtggctgc acctgccatt tccggtgctg agcgactccg acttgcgcct cgcgcgcgct
     1501 ctccgtcttc cgactttcga gatcgccggc atgatcctga tcaagaggct gaccctgatc
     1561 gttcgcgacg gcaggatcga gcatgttttc tatccggtgt ttccgccgga tcagggtgca
     1621 aacgaggtcc taagctggct cgccgcgcaa ccctagcccg gcgcatcgcc gcgcgaccct
     1681 agccccgcac ctcgccaatg aaggagagcg cgaccgtccg ccggtgcggg cttgcgcgat
     1741 gctcgatgag atagacgccc tgccaggtgc cgagcgccat cttgccgtca accaccggaa
     1801 tgccgaggct cacgttcgtc agcatcgact tgatatggct cggcatgtcg tcggggccct
     1861 cttcttgatg cgcgtagtcc gcgccctccg gcgcaagcgc ctcgagcgcg tcggtgaggt
     1921 cgcgatgcac attcgggtcg gcgttctcct ggatcgtgag agaggccgag gtatggcgca
     1981 cgaacacggt gaggaccccg ttcgcaccgc cgatctgttt cagccagctt gcaagcgcgc
     2041 cggtaatgtc gataaagccc ttgccttgcg tcgcaacgct gaggctgtgg ctcgcctgca
     2101 tcatctcggt catggcctag agttcgcctc cgtggagccc gttcgtgccc ccatggtcgc
     2161 ccgcgtgccg gcgccttgac aaggcttaag ccgcttcagc cacgaaggct gatgccagcg
     2221 gatcgcgtga cttctgtccc actctagggc gccgcttaac ccaaaattga ccggggcttg
     2281 ccaccgtgtt tcaggccgtt tcttcgcgcg tccgctttgg tgttcggacc aactcgggcg
     2341 cgcatttggg ccgtttgccc aaggtgaaca tcatgtcttc gcaagcaagt tggcagaccg
     2401 gcggcgcggg ctggggcgag ggtttcccgg acgagcaatc ggtcgagggc ctgcttcgcc
     2461 atctccttgt gcaggtcgag gagaccgaac gccgctacag cgatgccctc gatgagctgc
     2521 aagggcgcct cgaccagctt gcgctcaaga ccgatgccgc gcgctccggc gccacgggcg
     2581 gcggacaggc cctggaccgg ctgcacgatc aggtgagcgg gctcgcgcgg cgcttcgagc
     2641 gcgaagcttc cagctcgctc gacgatttcg agcgcctcgg ccgcgcggtg atgggtgggc
     2701 tcgatcggga cgcgagcggc ttctcgtttg atccttctgc gccgccctac acctcgccca
     2761 cgccggagcc gaactccgtc ttttcgtcgt tcccgccttt gcctgaagcc gaccgcgatc
     2821 tgagcaagcg cctgatcgag atggcgcatc gcctcgaaca ttccgtcgat gcggcaatga
     2881 cgccacgatc gctcgacgat ctgcccgcac ggatcgacgc ggtcgggcgg cagattgccg
     2941 atgcgctcgc cgccctgccc aagcccgttt cgctcgagcc tctcgaacac caaattgcct
     3001 atgtcgccca caagatcggt cgcgccgaga ccgagctggc caagatcggc gacatcgaaa
     3061 ccgcactcca tcgtctgatc gagcgcatgg atcgtcaggc aggccaactc ggcgacgtcg
     3121 cggccaaggc tgcaaccgag gccgcgcgtc tcgtttcagg cgaagcgagg ctcgacgcag
     3181 ccaccgccga acgtctcgat accatgcatc gcgacctcat ggcgatgaac gcgcgctcga
     3241 gcgccgccga cgaccggctc gccggcagca tcgaggcggt gcatcaatct ctcaagcagc
     3301 tcgtgcttga gaccgagcgg agtgcttcgc gggcgaccgc gctcagtccg cacgcgccct
     3361 tcccggagcg catgcctgac gcacatggcg agcaggcgga gagcgtatcg gcgaaaggcg
     3421 tagagtatca gatggagagg cgagcgccgc ccggcgttga ggcgaagcct cttgaggcga
     3481 agcttcctga ggcaagtcct cttgaggcga ggcctcttga ggcaaggtct ctcgaggcaa
     3541 ggcctccatt cggacgcgcc aaacgcgccg gatctggcga tacgctcgtc gatcttgacg
     3601 aagagagatc cgtcgatctc gacgttccgc tcggaagccg gagccttgtc aaaagagtcg
     3661 cgcgcacgga atcggagatg gaaaacgatc tcgttgctgc ggcgcgcagg gctgcccaag
     3721 ccgcggcgcg aagagcggcc gagcggacag gcagggacat gcgcaactgg gcgccggccg
     3781 gcgcgcgcac ttcgtcccgc gtcgagacgc tccgggccga gacgcctcgc ggcgccgaaa
     3841 cgcatcggtc cgaggcgctg tcgcggcgaa gccggcctct gctcatcgtc gcggcggcgg
     3901 ttcttctcgt cttgagcgcg atcctgctct atagccgctt gcaaacgaaa tcctggcccg
     3961 agtttctggc tcccgccgct gaggagagcg cgccgctacc ctcggcgcca attgaggaga
     4021 gcccccttcc cggcgcgggc gacaaggtgc ccgacgccga gcccgagaaa gagacggcgc
     4081 cagcgccggg ccccgtcccg cccgatgccg cagcgaaccc gccgcgcgat gccgccggcg
     4141 gtaccggtaa aagcgcgaac gtcaccgaca tcgccaaatc gacgtactgg ccggcgacgg
     4201 ttgtggagga gccggtagaa gccgcgcccg ccacgggtgt gcaagcaaca cggcaagccg
     4261 agcctgccgc cttgcgcgcc gccgaagagc ctctgcttcc gccgggggtt gtctttaccg
     4321 tcgaagaccc gtctcgcagc ttctaggcgc cgccgtcacg aagggcgccg ccacaatcga
     4381 tgatcgtgcc gcaagcagcc tccctcaggt aaggggaaag gttcggcacg ctctttccca
     4441 ctgatctttt ttgcgcgctg ctcctgccgg ttggaactcc aaggcccaga tgccggtttg
     4501 gtatcggggg acgctccact ggcgcaaggg gtgctaacgc caaagaacag aggggactgg
     4561 agcaatgacg aaactcgacc agctcaatgc cgagaagggc aagaagttcg gtgaaatatg
     4621 cgccttcatg gcgcgcgaac ttggctacgg tgatttcgcc agaataggtc ctgaagaccg
     4681 caagtatatc gagcaggaag cggagcagca gctcagtgat tgggaggaga cgaccgaatt
     4741 ccggcttcgt cccgatattc ggccaagcac gcctttgcgc cgtctcttga gcgagcacca
     4801 gcacatctgc gaaagaatcc tcgatgagca ggacatcgag atcggtctgt gggcctacaa
     4861 gaagagaaag acgggacgcg ggcgggtgtc tctctaagaa cctggcctct ctctaagagc
     4921 ctggaaaacc agcagagcgc cggtctcggt cggcgccttc ctgtgcaaca aacggcacgc
     4981 tatgcccttc gggaagggac gcgcgccgcc cgcggtgaga ggtaagcggc tcttaaccag
     5041 ataatgctcc tcgcttgtac gggaagcgtt ttagagagca agatcccttg acctcacagg
     5101 ttcaagaggc tcgcgtcgac gcggcaatgg acctttcaag acggtgagtc tcgggtaccg
     5161 cctccgccga cgctggcgcg gttggagagt cacgctgctt tcgcggctga cggcacgtct
     5221 ccgatgtgcc attaacgggc ggtacaagcc gaagcgatag tctcccgccg ttgtgtccgc
     5281 gagacatttt agaatcctga tcccaaccct tcctcatgcc agagaccaaa ccgcatgggc
     5341 cgacggtgat cccgtggcag cctttcgccg agacaagtgg tagcgacacc ggcaggagct
     5401 tgaagtgacc attgcccccg atcccggcgc ttacgattcg gccgtggtca ccgattttct
     5461 gctgttcggc gccacgctcg cggccgtcgc gatctttcac cgccacacgc tcgccgccgc
     5521 cctcatcggt cttggcgcca tcatcatcaa gaagctcttg ggcgaaggct tcgctgaagg
     5581 cgcgggcctc tccggcctct tgcccatttc gcatatgaat gggtgctgct tgcgaacttg
     5641 tttctgcttc tggtcggctt cgcc
//
LOCUS       DASUQP010000034         4021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1650759, whole genome shotgun sequence.
ACCESSION   DASUQP010000034 DASUQP010000000
VERSION     DASUQP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650759"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3495
                     /locus_tag="VFY74_01695"
     CDS             <1..3495
                     /locus_tag="VFY74_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5930451.1"
                     /translation="AVMLSSVNAYLAGVDESLAQPGTFDRASLEPLWNSAAEDALTAW
                     TGGLTDLRGLLDQRVGDLMGRLYWSLLIMGTLAALSILLAIITHRNIVRPLARLEDLA
                     NRVRQTKDYTHRVDYESKDEIGHLAAAFNEMLGELDQARTRELADQADQAARKRLSVL
                     LDASPAVIYCRLARGDFEPTFVSESVTRLFGCTPREYLENSNLWRDRVHPDDVPRIAD
                     WVDRMFESDKRALEYRIRRPDGSYFWVNDEQHIVRDAKGEPVEIVGSWTDITERKEAE
                     HARERARQRLDLLLSAAPVVVYSFAATGDYAPTFVSASIGPMLGYGPEEYLEDAHFWR
                     SRVHPDDLAEIEAKQVELFQHGDHHVEYRFRKKDGAYCWVSDEQHVIRDPNGRPVEVV
                     GSWSDIDARKAAEQAFQAAQEELKKATAAALEASEAKSAFLANMSHEIRTPMNAVIGL
                     SHLALKTDLTPRQRDYVLKIKSSGQHLLGIINDILDFSKIEAGKLSIENIDFDLDKVL
                     ENVGNLMSEKASAKGLELIFEVEPTVSTHFRGDPLRLGQILINFCNNAVKFTERGEVA
                     VKVRVLEDSADSQLVEFSVSDTGIGMSEQQVARLFQAFEQADASTTRKYGGTGLGLAI
                     AKQLTELMGGEVSVTSAPGKGSTFRFTARLGKGIAIPRPRLLQSDLSGRRVLIIDDNP
                     HARAVLATMLTNMSLVADEAASGEEAIAMIRQAAKARKGYEIVFIDWQMPGMNGIETG
                     KQIFALADLDPAPHLVMVTAYGREEVLKQAEESGFENVLIKPVTSSILFDTAVVALGA
                     ASGRAETAPAMPSFEIDHMRGARILLVEDNEINQEVAIGQLEDAEAFVDLAENGAIAL
                     EMIKANDYDAVLMDMQMPVMGGIEATRVIRSNPRFKKLPIIAMTANAMASDRILCLEA
                     GMSDHIAKPIDPDQLFGVLLRWIRRTGGDGGAARGREAPPQARKGGGETELVIPGIDF
                     AAGLKRTGGNRKRYETLLRKFAEQQSGTSAAIGSALAEGDTATAERAAHSLKGAAATL
                     GAKALSEAASHAEAAIKNGNDAGPEVQTLTRSLEAVLADLRAALPGEAGGNGSGAPVS
                     DPARVQEPLITLKRLLETDDGEAADFIVDAKPRLSGVLTAAEINALTERVGKFDFDAA
                     LKCLWGIAARLSLDLEAK"
     gene            3495..>4021
                     /locus_tag="VFY74_01700"
     CDS             3495..>4021
                     /locus_tag="VFY74_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5930452.1"
                     /translation="MSGPEKKLVLIVDDTPTNVAVVSGVLKDSFRTKVATNGEKALAI
                     AGGVEKPDLILLDVMMPGMDGYEVCRRLKDNPATRDIPIIFLTAKTEEVDEEKGFDVG
                     AVDYIHKPFSAPIVLARVRTQLALQEALIEARDARNQADQLLHALLPKIAADEIRSIG
                     TVIPRRYENVAVLFC"
BASE COUNT          806 a         1281 c         1259 g          675 t
ORIGIN      
        1 gccgtgatgc tgtccagcgt gaacgcttat ctcgccggcg tcgatgagag cctggcccaa
       61 ccgggcacgt tcgatcgcgc ctcgctcgag ccgctgtgga acagcgcggc ggaggacgcg
      121 ctgacggcct ggacgggcgg attgaccgat cttcgagggc ttttggacca acgcgtcggc
      181 gatctgatgg ggaggcttta ctggagcctt ctcatcatgg ggaccctcgc cgcgcttagc
      241 atcttgctcg ccatcatcac ccaccggaac atcgtgcgtc cgctcgcgcg actcgaagat
      301 ctggcaaacc gcgtccggca gaccaaggac tacactcacc gcgtcgacta cgaaagcaag
      361 gatgagattg ggcacttggc ggcggccttc aacgagatgc tgggtgaact cgatcaggcg
      421 cgcacgcgcg aactcgcaga ccaagctgac caagccgcgc gcaaaaggct gagtgttttg
      481 ctcgatgctt cgcccgccgt catctactgc cgtttggcca gaggcgattt cgagccgacc
      541 ttcgtcagcg aaagcgtgac ccggctgttc ggctgcacgc cgcgcgaata tctcgaaaat
      601 tccaacctgt ggcgagatcg cgtccaccct gacgacgtcc cgcgcatcgc cgactgggtc
      661 gaccggatgt tcgagagcga caagcgcgcg ctcgaatatc gcatccggcg ccctgacggc
      721 tcctatttct gggtgaatga cgagcagcac atcgtccggg acgccaaggg cgagcccgtc
      781 gagatcgtgg gttcgtggac cgacatcacc gaacgcaagg aggctgaaca cgcccgcgaa
      841 cgcgcaaggc agaggctcga tctcctgctc agtgccgctc cggtcgtggt ctacagcttc
      901 gccgcgaccg gcgactacgc gccaacgttc gtcagcgcca gtatcggacc gatgctgggc
      961 tatggcccgg aagaatacct cgaggatgcc catttctggc gaagccgcgt tcatcccgac
     1021 gatttggcgg agatcgaagc caagcaggtc gagctcttcc agcatggcga tcatcacgtc
     1081 gagtatcgct ttcgcaagaa ggacggcgct tattgctggg tcagcgacga acagcatgtc
     1141 atccgtgacc cgaacggtcg tcccgtcgag gtcgtgggct cttggagcga catcgacgcg
     1201 cgcaaagcgg ccgagcaggc ctttcaggcc gcccaggaag agctgaagaa ggcaaccgcc
     1261 gcggcgttgg aagccagcga ggccaagagc gccttcctcg ccaatatgag tcacgaaatc
     1321 agaacgccga tgaacgccgt catcggcctt tcccacctcg cgctcaagac ggacctcacg
     1381 ccgcgccagc gggactacgt gctcaagatc aagagctcag gccagcatct tctcggcatc
     1441 atcaacgaca tcctcgattt ctctaagatc gaggccggca agctgtcgat cgaaaacatc
     1501 gacttcgatc tcgacaaggt gctcgagaat gtcgggaacc tgatgagcga gaaggcctcg
     1561 gcaaaggggc ttgagctcat cttcgaggtc gagcccacgg tctcgaccca tttccgcggc
     1621 gatccgctcc ggctcggtca gatcctgatc aatttctgca acaacgcggt gaagttcacc
     1681 gaacgcggcg aagtcgctgt caaggtccgc gttctggagg acagcgccga cagccagctc
     1741 gtcgagttct cggtaagcga taccgggatc ggcatgagcg agcagcaagt ggcgcggctg
     1801 tttcaggctt tcgagcaggc cgatgcttca accacacgga aatatggggg cacgggcctt
     1861 ggcctcgcca tcgccaagca gctcaccgaa ttgatgggcg gggaggtttc tgttacgagc
     1921 gcgcccggca agggcagcac gttccgcttc accgcccggc ttggcaaagg gatcgccatt
     1981 ccgcgcccgc gtctcctcca atccgacttg agcggccggc gcgtgctcat catcgacgac
     2041 aacccgcacg cccgtgccgt cttggcgacc atgctgacca acatgtcgct cgtcgctgac
     2101 gaagcggcct cgggcgaaga ggcgatcgcg atgatccgcc aagccgccaa ggcgcggaaa
     2161 ggctacgaga tcgtcttcat cgactggcag atgccgggca tgaacggaat cgaaaccggc
     2221 aagcagattt tcgcgttggc cgacctcgac cccgcccctc atcttgtgat ggtgaccgcc
     2281 tatggccgcg aagaggttct gaagcaggcc gaggagagcg gctttgagaa cgtgttgatc
     2341 aagcctgtca cgtcgtcgat tctgttcgat accgccgtcg ttgctctcgg cgccgcctcg
     2401 ggcagggccg agaccgcgcc cgccatgccg tccttcgaaa ttgaccacat gcgcggcgcc
     2461 cgaatcctcc tggtcgagga caacgagatc aaccaggaag tggcgatcgg ccaactcgag
     2521 gatgcggaag ccttcgtcga cctcgcggag aacggcgcca ttgcgctcga aatgatcaaa
     2581 gccaacgact atgacgccgt gctgatggac atgcagatgc cggtcatggg cggcatcgag
     2641 gccacgcgcg ttatcaggtc caatccgcgc ttcaaaaaac ttccgatcat cgccatgacc
     2701 gcgaatgcga tggcctccga ccgaatcctc tgtctcgaag cgggcatgag cgaccatatc
     2761 gccaagccga tcgatcccga ccagctgttc ggcgtcctgt tgcgctggat ccggcgaacc
     2821 ggcggcgacg gtggcgccgc gcgcggcagg gaagcgccac cccaagcaag aaagggcggc
     2881 ggcgagacgg agctcgtcat cccgggcatc gacttcgccg caggcttgaa gcggacgggc
     2941 ggcaaccgca aacgctacga gacgctgttg cgcaagtttg ccgagcagca gagcggaacg
     3001 agcgccgcca tcggctccgc gctggccgag ggcgacacgg cgaccgccga gcgcgcggct
     3061 cattccttga agggcgcggc cgccacgctt ggagcgaaag cgttgtcaga ggccgcctcc
     3121 cacgccgagg cggcgatcaa aaacgggaac gatgcgggac ctgaagtaca aacgctcacc
     3181 cgttcgcttg aggccgtgct tgccgacctt cgcgccgccc ttcccggcga ggcaggcggc
     3241 aacgggtccg gcgcgcccgt gagcgacccc gccagagtgc aggaacccct cataacattg
     3301 aaacggttgc tcgagaccga tgacggcgag gccgccgatt tcattgtcga tgccaagccg
     3361 cgcctaagcg gagttttgac ggccgcggag atcaacgcgc tgaccgaacg ggtcggcaaa
     3421 ttcgatttcg atgccgcgct caagtgcctg tggggcatcg ccgcgcggct ctccctcgac
     3481 ctggaagcca agtgatgtcg ggacccgaga agaagctggt actgatcgtc gacgacacgc
     3541 cgacaaatgt tgcggtcgtc tccggcgtgc tgaaggattc gttccgcacc aaggtcgcga
     3601 ccaatggcga gaaggcgctt gcgatcgcgg gcggcgtcga aaagcccgac ctcatcctgc
     3661 tcgacgtgat gatgcccggc atggacggct atgaggtgtg ccggcgcctg aaggacaatc
     3721 ccgccacgcg cgacattccc attattttcc tcaccgccaa gaccgaggaa gtcgacgagg
     3781 aaaagggctt cgatgtcggc gccgtcgatt acattcacaa gccgttctcg gcgccgatcg
     3841 tcctggccag ggtcaggact cagctcgcgc ttcaggaggc attgattgag gccagggacg
     3901 cgcgcaacca ggcggaccag ctgctgcacg cgctgctgcc gaagatagcc gccgacgaga
     3961 tccgcagcat cggcacggtg attccacggc gttacgagaa tgtcgcggtc ctgttctgcg
     4021 a
//
LOCUS       DASUQP010000035         6468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_969715,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000035 DASUQP010000000
VERSION     DASUQP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969715"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1026)
                     /locus_tag="VFY74_01705"
     CDS             complement(94..1026)
                     /locus_tag="VFY74_01705"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX5930453.1"
                     /translation="MAKSKKSSKPKPLSFQDLILKLERFWADQGCVILQPYDLEMGAG
                     TFHPATALRSLGPKPWRAAYVQPSRRPKDGRYGENPNRLQHYYQFQVILKPSPSDIQD
                     LYLQSLFALGIDPAIHDIRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQQVGGF
                     DCNPASGELTYGLERLAMYVQDVDRVFDLDFNGGEGKDKITYGDVFLQAEQEYSRYNF
                     EQADTQILLRHFRDAEAECKALLASGDKGGRHLLALPAYDQAIKTSHIFNLLDARGVI
                     SVTERQAYILRVRDLAKACCAAWLKTEAGGAANG"
     gene            complement(1327..1548)
                     /locus_tag="VFY74_01710"
     CDS             complement(1327..1548)
                     /locus_tag="VFY74_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930454.1"
                     /translation="MPQLLILAAVGAGLLLAGRVYRQMQKRIQSELRAAEEALARRAR
                     ETAVPLERDPATGIYRPKRMAQGSERTRH"
     gene            complement(1552..2454)
                     /locus_tag="VFY74_01715"
     CDS             complement(1552..2454)
                     /locus_tag="VFY74_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX5930455.1"
                     /translation="MSTLFDEVGPPASASWKSYLPESWTKSGPIVPVLRFAGAIGVVT
                     PLRPGLSLSTSASAIEKAFTMRGAKAVAIQINSPGGSAVQSMLIYKRIRALAAEKNLK
                     VYVFAEDVAASGGYLLALAGDEIYADPSSIVGSIGVITATFGLNELINRIGVERRVYT
                     AGANKDILDPFLPEKAEDVERIKALQRDVHQSFIDLVKTRRGAKIDKAGIDLFTGEFW
                     SGGKALELGLIDGLSDLRTKMREIYGDEVRLKPVSSATSWFRRRRGVLAEGEGLGFGF
                     SPGGFAADLISAIEARALWSRFGF"
     gene            2684..2929
                     /locus_tag="VFY74_01720"
     CDS             2684..2929
                     /locus_tag="VFY74_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930456.1"
                     /translation="MSKLATLAVAVGALALAFVTLAPSSAEAGWRRGYRGWGPGVSVY
                     VGPRWGWYGPRRYWRPRYPVGYPYAYYPYWRYRYYNW"
     gene            complement(3017..3844)
                     /locus_tag="VFY74_01725"
     CDS             complement(3017..3844)
                     /locus_tag="VFY74_01725"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5930457.1"
                     /translation="MSSPTTSKAKRGARESATSTDDFLGGCISVVQPRGGHRAGSDAV
                     FLAASVPALAGERVLDVGAGAGVAGLCLLARVSGVHVTAVEIDRRLAALAARNAARNG
                     FADHFRAVTADVRSPGRALSEAGLKREGYHHVIANPPFYAGTRVRAAPDSARATAHVM
                     PDGGLSDWVSLFATMARPNGLLTLIHRPDCLEELLSVLYGRFGGIAVFPLFPRAGEAA
                     TRIIVQAKKGSRTGLSLLPGLVLHEADGRYTEAAEAVLRGGDALELNRSSDGKSPER"
     gene            complement(3816..4049)
                     /locus_tag="VFY74_01730"
     CDS             complement(3816..4049)
                     /locus_tag="VFY74_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX5930458.1"
                     /translation="MKELLRSNDPVLISYVSALLEQERIDFIVADLNMSVLEGSIGAL
                     PRRVLVESDKIAQARQILTGAGIGHVIADDEQG"
     gene            4144..5160
                     /locus_tag="VFY74_01735"
     CDS             4144..5160
                     /locus_tag="VFY74_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5930459.1"
                     /translation="MGVVVKLAGGSDPGASLAPLVQLVEEDMAHVNRIILDRARSNVE
                     LIPELARHLIDSGGKRLRPMLTLAAAKLCGYEGDGHVRLAASVEFMHTATLLHDDVVD
                     ESSLRRGKASARLVWGNEASVLVGDFLLGQAFKMMVDVGSVPALRILSDAAAVIAEGE
                     VMQLVTAKNTETTEDDYLAVIDAKTAALFSAAAEVGAAIADRPKSEAAALRSYGRNLG
                     LAFQLVDDALDYAGEPATLGKSVGDDFREGKITLPVVLCYRRGNATERAFWRRTLNDG
                     EIGEGDLAEAMSLMKRHNALSDTLDRARHYGAIARDALAIFPESAAKAALREVIAFCV
                     DRPF"
     gene            complement(5157..6026)
                     /locus_tag="VFY74_01740"
     CDS             complement(5157..6026)
                     /locus_tag="VFY74_01740"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX5930460.1"
                     /translation="MTALRDIGRAKINLTLEVLGRRSDGFHELKSLVAFASFGDDLEL
                     DPGGGLELEVEGPFAAALGGDNLILDAACAARASVTGLRLGRFHLGKLLPVAAGLGGG
                     SADAAAALRLIARANPGLLRESALARLAAALGSDVSVCLNSRPALMTGRGEKVEPVSG
                     FPPCGVVLANPGRALATQAVYAALHAEPLGAEPQHGAEKLDFHDDFAALLAYALPRRN
                     DLEGPAANLLSDITGVLAALSALKGARLARLSGSGPTCFALLAREEEARQGAAHLAAQ
                     HPAWWVASGALEV"
     gene            complement(6061..>6468)
                     /locus_tag="VFY74_01745"
     CDS             complement(6061..>6468)
                     /locus_tag="VFY74_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5930461.1"
                     /translation="NYLGYSWVDQGRNLKQAMEYIRRAVKLKPDDGYYVDSLGWAYYR
                     LGNLAAAVEHLERAVELKPDDPIINDHLGDAYWKVGRKLEAKYQWQQSLTLKPEADLV
                     VTIKKKIELGLQENDVETKSAQDRTRQAGQKTQ"
BASE COUNT         1120 a         2066 c         2120 g         1162 t
ORIGIN      
        1 ttcagcctgt cgccgagcag gcgcgcaaga tcctcgcggg cgcggccttg catgcgcgcc
       61 gggatctcct cggagaacaa ctcgatcagg agttcagcca ttggcggcgc cgcccgcttc
      121 ggtcttgagc catgcggcgc agcacgcctt ggcgaggtcg cgcacgcgca agatataggc
      181 ctgccgctcg gtgaccgaga tcacgccgcg cgcatcgagc agattaaaaa tgtgcgacgt
      241 cttgatcgcc tggtcgtagg cgggaagcgc gagaagatgg cgaccgcctt tgtcgccgct
      301 cgcgagcaat gccttgcatt cggcttcggc gtcgcggaaa tggcgtagca ggatttgcgt
      361 gtcggcctgc tcgaaattgt agcgggagta ttcctgctcg gcctggagaa agacgtcgcc
      421 gtaagtgatc ttgtctttgc cctccccgcc gttgaaatcg aggtcgaaca cgcggtcgac
      481 gtcctgcaca tacatggcga ggcgctcgag cccgtaagtc aactcgcccg aggcgggatt
      541 gcaatcgaag ccgccgactt gctggaagta ggtgaactgc gtcacttcca ttccgtcgag
      601 ccacacctcc cacccgagcc cccaggcgcc gagcgtcggg ctctcccaat cgtcctcgac
      661 gaagcggatg tcgtgtattg cgggatcgat gccgagagcg aagaggctct gaagataaag
      721 gtcctggatg tcggacggcg acggcttcag aatcacttgg aactgataat agtgctggag
      781 ccggttcggg ttttcgccat agcggccgtc cttggggcgg cgggagggct gcacgtaagc
      841 cgcgcgccag ggcttgggcc cgagcgagcg cagcgcggtc gcgggatgaa aggtccctgc
      901 ccccatctcg aggtcgtaag gctggaggat cacgcagccc tgatcggccc agaagcgctc
      961 cagcttgagg atcaagtcct ggaaggagag aggcttcggc ttcgaggatt tctttgactt
     1021 ggccatccgt tggaccgctc gatcggtgaa gccttggcgg ggtcgcgggg acgctaatgc
     1081 aattcgtgcg caaattgaac cgtcatgccg ggcccgtggc taaaaaccgt ttccacgtca
     1141 tgcccggaca cccgggtcct gcttcgccgg ccagggcgca agcttgtctg catccatgcc
     1201 gttacatctc agcgggctga agcgccattg gaatggattg ccggagcctg tccccggact
     1261 tgatccgggg atcaagcccg gcaatgacga aaggaggaaa tgctgaaagg ttaccagact
     1321 tgggtgctaa tgccgcgtgc gttcggagcc ttgcgccatc cgctttggcc ggtagatccc
     1381 ggttgcgggg tcccgttcca gcggcacggc ggtttctcgc gcgcgccgcg caagcgcctc
     1441 ttcggcggcc ctgagttccg attggatgcg tttctgcatc tggcgataga cgcgaccggc
     1501 gaggaggagg ccggcgccca cggcggcaag gatgaggagc tgcggcatcg ctcagaaccc
     1561 gaagcgcgac cagagcgcgc gggcttcgat cgcagagatg aggtcggcgg caaacccgcc
     1621 gggcgagaaa ccgaacccca agccctcgcc ttcggcaaga acgccgcgcc ggcggcggaa
     1681 ccaggacgtt gcggacgata ccggcttgag acgaacctcg tcgccgtaga tctcccgcat
     1741 cttggtgcgg aggtcagaga gcccgtcgat caggccaagc tcgagcgctt ttcccccgga
     1801 ccagaactcg ccggtgaaga gatcgatgcc ggccttgtcg atcttcgcgc cgcgccgcgt
     1861 cttgacgaga tcgatgaagg attgatgcac gtctcgctga agcgccttga tgcgctcgac
     1921 gtcttccgcc ttctcgggga ggaacggatc gaggatgtcc ttgttcgcgc ccgcggtgta
     1981 gacgcggcgc tcgacgccga tgcgattgat cagctcgttc aagccgaacg tcgcggtgat
     2041 gaccccgatg gagcctacga tcgaactcgg atcggcatag atttcatcgc ccgcaagcgc
     2101 gagcagatag ccgccggaag cggcaacgtc ctcggcgaag acatagactt tgaggttctt
     2161 ctcggcggcg agcgccctga tgcgcttgta gatcagcatc gactgcaccg ccgagccgcc
     2221 gggcgagttg atctggatgg cgacggcctt ggcgccgcgc atggtgaaag ccttctcgat
     2281 cgccgaggcc gatgtggaga gcgacagtcc ggggcggagc ggcgtgacga cgccgatcgc
     2341 gcctgcgaag cggaggacgg gaacgatggg tccgctcttt gtccacgatt cgggaagata
     2401 gctcttccag ctcgcgctgg ccggggggcc gacctcatca aacagagtgc tcatgacggt
     2461 ttacgtatag ctggctcacg tcaaagcaaa ggctgagcac cgaattctgt gccaattaag
     2521 accgccgggt gcgccttaag ccttggttag cgcaagtcgg agactgtcat cgggcagccg
     2581 aattcccggc ctatagttca ctccgaaggc ggcatgcaac ggcgccctcg gcggcgcgtc
     2641 cggcggatgg cgagcgccgc ttccgatctc cgaggaggaa tgaatgagca agcttgcaac
     2701 tctggccgtg gcggtgggag cgcttgcgct cgcctttgtc acgcttgccc cgagttcggc
     2761 ggaagccgga tggcgccggg gctatcgggg ctggggaccc ggggtgagcg tttacgtcgg
     2821 gccgcgctgg ggctggtacg gtccccgccg ctattggcgc ccgcgatacc ccgtaggcta
     2881 cccttatgcc tattatccct attggcgtta ccgctactac aattggtaac aggcacgcgc
     2941 gcctccatgg cgcctgccag tcgcgaaaga gccgctccct ggaaagggtt cggctctttg
     3001 gttctcaagc ggagacctat cgttcgggac tctttccgtc actcgatcgg tttaattcga
     3061 gtgcgtcgcc tcccctcagc accgcttcgg ccgcttccgt gtaacggcca tcggcctcgt
     3121 gcagcacaag cccgggcaga aggctgagcc ccgtccggct tcctttcttc gcctgcacga
     3181 tgatacgcgt tgccgcttcg cctgccctcg gaaagagggg gaagacggca atgccgccga
     3241 accgcccgta gagcacggag agaagttctt cgagacagtc cggccggtgg atcaaagtca
     3301 gaagcccatt tggccgagcc atcgtggcga acaagcttac ccagtcggac aggcctccat
     3361 caggcatgac atgcgcggtt gcgcgcgcgg aatccggcgc cgctctcacc ctcgttccgg
     3421 cgtagaaggg cgggttggcg ataacatgat ggtagccctc ccgcttgagt cccgcttcgc
     3481 tcagtgccct tccgggagac cttacgtcgg ccgtgacggc ccggaagtga tctgcgaaac
     3541 cgtttcgtgc cgcgttcctt gcggcaagcg cagccaatcg ccgatcgatc tcgacagcgg
     3601 tcacgtgcac gccgctgacg cgcgccaaga ggcaaagccc ggccacgcct gcgcccgcgc
     3661 cgacatcgag cacgcgttcc cccgccagcg caggtacgga cgccgccagg aacaccgcat
     3721 cgcttcccgc ccgatgcccg ccgcgcggct ggaccacgct gatgcagccg ccgaggaagt
     3781 cgtcggttga ggtggcgctt tcccgcgcgc cgcgcttagc cttgctcgtc gtcggcgatg
     3841 acatggccga tgccggcccc ggtgagaatt tgtcgcgcct gcgcgatctt gtcgctctcg
     3901 accaagacgc gccgaggcaa agctccgatc gagccctcga gcacgctcat attgagatcg
     3961 gcgacgatga aatcgatgcg ctcctgctcc agcaaggcgc tgacatagga gatgagcacc
     4021 ggatcgttcg atcgcagcaa ttccttcatg gcttggcggg cctcctcgtc ctaggtggat
     4081 cgccttgacc tcgacggtga tgcgccgaca ttatcaaggc cagttgcgtg gaggagatgt
     4141 cctttgggcg ttgtggttaa gcttgccggc gggagcgatc ctggcgccag tctcgcgccg
     4201 ctcgttcagc ttgtcgagga ggacatggcc catgtcaacc gcatcatcct cgaccgggcg
     4261 cgttcgaacg tcgagctgat ccccgagctt gcgcggcatc tcatcgattc gggcggaaag
     4321 cgcttgaggc ccatgctgac gctcgccgcg gccaagctct gcggctatga gggcgacggg
     4381 catgtgcggc tcgcggcatc cgtcgagttc atgcacaccg cgacgcttct tcatgacgac
     4441 gtggtggatg agagcagcct gcggcggggc aaggcctcgg cgcgcctcgt gtggggcaac
     4501 gaggcgagcg tgctcgtggg cgatttcctg ctcggccagg ccttcaagat gatggtggat
     4561 gtcggctcgg ttccggcgct tcgcatcctg tccgacgccg cggcggtgat cgccgagggc
     4621 gaggtcatgc aactcgtgac ggccaagaac accgagacca ccgaggacga ctatctcgcg
     4681 gtcatcgacg ccaagaccgc ggcgctgttc tcggctgccg ccgaggtcgg cgcggcaatc
     4741 gcagataggc cgaaatcgga ggccgcggcc ttgcggtcct acggccgcaa tctcggtctc
     4801 gccttccagc tcgtcgacga cgcgctcgac tatgcgggcg agcccgccac gctcggaaaa
     4861 agcgtgggcg acgatttccg cgagggcaag atcacgctcc cggtcgtgtt gtgctaccgc
     4921 cgcggcaacg cgacggagcg ggcgttctgg cgccgcacgc tgaatgacgg cgagattggc
     4981 gaaggcgatc tcgccgaggc gatgagcttg atgaagcgcc acaatgcgct gtccgacacg
     5041 ttggacaggg cacggcacta tggcgccatc gcccgcgacg cgctcgccat cttcccggag
     5101 agtgccgcca aagcggccct gcgcgaggtc atcgcgttct gcgtcgaccg cccgttctag
     5161 acttcgagcg cgcccgaggc gacccaccag gccggatgct gggcggcaag atgagcggcg
     5221 ccttgtcttg cttcttcctc ccttgccagg agcgcgaagc aggtcggccc gctccccgat
     5281 agcctggcga gtcgagcccc tttgagcgcc gaaagcgcag caagcacgcc tgttatgtcg
     5341 gaaagaagat ttgcggccgg tccttcgagg tcattcctgc gcgggagcgc ataggcaaga
     5401 agtgcggcga agtcgtcgtg aaaatcgagc ttctcggcgc cgtgctgcgg ctcggcccct
     5461 aacggctccg cgtggagggc ggcgtagacg gcctgggtgg caagagcccg gccaggattg
     5521 gcaagaacga ccccgcaagg cgggaagccg cttacgggct cgaccttctc gccgcggccg
     5581 gtcatgaggg cgggccggct gttcaagcag acgctcacgt ccgagccaag cgcggccgca
     5641 agtcgcgcca aggcgctctc ccgcaacaac cctggatttg cacgcgcgat cagccgcaaa
     5701 gctgcggcgg cgtcggcgga gccgccaccg agacccgcgg cgacgggaag aagctttcct
     5761 aggtggaacc ggccgagcct caggcctgtc acgctcgcgc gcgcggcgca cgccgcatcg
     5821 agaatgagat tgtcgccgcc gagagcggcg gcgaaggggc cctcgacctc aagctcgagg
     5881 ccgccaccgg ggtcaagctc gaggtcatcg ccgaagctcg caaaggcgac gagactcttg
     5941 agttcgtgaa agccgtcgga gcggcgcccc agcacttcca gcgtgagatt gatcttggcc
     6001 cggccgatgt ccctgagcgc cgtcatgcac gctcaccccg catgctccgg ccatggcgct
     6061 ctattgggtc ttctggcccg cctgcctggt acggtcctgc gccgacttgg tctcgacgtc
     6121 gttttcctgc agtcccaact cgatcttctt cttgatcgta accaccagat ccgcttccgg
     6181 cttcagcgtc agcgactgct gccactgata cttcgcctcg agcttccgcc ccaccttcca
     6241 ataggcgtcg ccgaggtgat cgttgatgat gggatcgtcc ggcttgagct cgacggcgcg
     6301 ttcgagatgc tcgaccgccg ctgcgagatt gccgagccgg tagtaagccc agccgagact
     6361 gtcgacgtag tagccgtcgt caggcttcag cttcaccgcc ctgcggatgt actccatcgc
     6421 ctgcttcaag ttccggccct ggtcgaccca ggaatagccg agatagtt
//
LOCUS       DASUQP010000036         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1787434, whole genome shotgun sequence.
ACCESSION   DASUQP010000036 DASUQP010000000
VERSION     DASUQP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787434"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFY74_01750"
     CDS             <1..811
                     /locus_tag="VFY74_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX5930462.1"
                     /translation="IATTLIDGRDLGAWEAAVKPNTRAFFFETPTNPVLELVDIEGVA
                     KIAKRAGALVVVDNVFATPLLQRPMPLGADIVVYSATKHIDGQGRCLGGVVLGPEEYI
                     EEKLHNYLKHTGPSLSPFNAWVLLKGLETLPLRVERHGTSAAAIADFLAAQPEIVRVF
                     YPGRADHPQAALAKRQMDGGGPMVAFEIEGDKKSAFHFMNALQIFKISNNLGDAKSLV
                     THPATTTHQRLTPEARAELGIFDNSVRLSIGLEDIRDLMADLDRALSVSRG"
     gene            909..1178
                     /locus_tag="VFY74_01755"
     CDS             909..1178
                     /locus_tag="VFY74_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX5930463.1"
                     /translation="MCRNIKTLYNFDPPASDEEIYLSSLQFVRKLSGFSKPSTANQAA
                     FDRAVDKVSRAARELIDTLVTTAPPRDRDVEALKARARSAERFPG"
     gene            complement(1410..1574)
                     /locus_tag="VFY74_01760"
     CDS             complement(1410..1574)
                     /locus_tag="VFY74_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930464.1"
                     /translation="MTLFYAEYFDRITDAVSAERQIKGWSRAKKEALASGNFEALVVL
                     ARRHRSKPAI"
     gene            1853..3010
                     /gene="argE"
                     /locus_tag="VFY74_01765"
     CDS             1853..3010
                     /gene="argE"
                     /locus_tag="VFY74_01765"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEX5930465.1"
                     /translation="MSSQNATATELLRALVAFDTTSAKSNLALIAFVENYLKGHGIAA
                     TRVSSPDGAKADLFATIGPKGDSGIGLSGHSDCVPVEGQRWTSDPFTLTERGSKLYGR
                     GSCDMKGFIACALAAVPSFKARALKEPIHIIVSYDEEVGCTGVRPLIARLGKDLPKPR
                     AVIVGEPTGMAVIDAHKRIDAYRTHVTGREAHSSMPELGVNAIAAAAALIGELDRIGG
                     EIAKREKDKRFEPPYSTVCVGTIEGGTAHNIVPKTCAFQWQVRSLPSVEPGEVPRGLA
                     AFAETLLPKMRQVTKEAAIDTEGEGSVPAFVAAGGSEAVALALALTGADHTEAVSYTT
                     EAGLFEEASVPAVICGPGNIAQAHAADEYVTIAQLEACLAFLDGLAARLST"
     gene            complement(3014..>3324)
                     /locus_tag="VFY74_01770"
     CDS             complement(3014..>3324)
                     /locus_tag="VFY74_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX5930466.1"
                     /translation="FTQEDWTRAKALAETVEDHELLDPTLPPDRLLYRLFHEEQVRAY
                     RAVPLKGYCSCSEARVDELLRRFSAEDLAEMVVDGEVSVTCEFCNRRYRFDPATFADA
                     "
BASE COUNT          632 a         1092 c         1031 g          569 t
ORIGIN      
        1 catcgccacg acgctgatcg acggacgcga tcttggcgcc tgggaagcgg cggtaaagcc
       61 gaacacgcgc gccttcttct tcgagacgcc gaccaatccc gtgctcgagc tcgtggacat
      121 cgaaggcgtg gcgaagatcg cgaaacgcgc cggcgcgctt gtcgtggtcg acaatgtgtt
      181 cgccacgccg ttgttgcagc gaccgatgcc gctcggcgcc gacatcgtgg tctattcggc
      241 gacaaagcat atcgacggcc agggccggtg cctcggcggc gtcgtgctcg gacctgagga
      301 gtacatcgag gagaagctcc acaactacct caagcacacc ggaccctctt taagcccgtt
      361 caatgcctgg gtgctgctga aggggttgga gaccctgccg ctcagggtcg agcgtcacgg
      421 cacctcggcc gccgcgatcg ccgattttct tgccgcgcag cccgaaatcg ttcgcgtctt
      481 ttatccgggc cgcgcagatc acccgcaagc ggcgctcgcc aagcggcaga tggacggcgg
      541 cgggccgatg gtcgccttcg agatcgaggg cgacaagaaa agcgcgttcc acttcatgaa
      601 tgcgctccag atcttcaaga tcagcaacaa tctcggcgac gccaagagcc tggtcacgca
      661 ccctgcgacg acgacgcacc agcgcctcac gccggaggcg cgggccgagc tcggcatttt
      721 cgacaacagc gtgcggctct ccatcgggct cgaggacatt cgcgacctca tggccgatct
      781 cgaccgagct ctttccgtct cacgcggcta gacgagacgt cgcgtctggc ggtcgtcctc
      841 gtcttgtttc gcagagatcc atgtgccacg ctttgcaacg acaatgactg aagtgatgag
      901 gttttgtcat gtgccggaat atcaagacgc tttacaattt cgacccgccc gcatcggacg
      961 aggaaatcta tctctcgtcc ctgcaattcg tgcggaagct gagcggcttc tcgaaaccgt
     1021 cgacggccaa tcaggccgcc ttcgatcgcg cggtggacaa ggtctcgcgc gccgcccgcg
     1081 agctgatcga tacgctcgtc acgacggcgc cgccgcgcga tcgcgacgtc gaagcgctga
     1141 aggcgcgcgc ccggtccgcc gagcgcttcc ccggctgaaa ccaatacctc atgctgagga
     1201 gccgccgaag gcggcgtctc gaagcatgac ccgaccgcac cccagggcca attttggcaa
     1261 catcttaaac ctcacccttc gagacggcgc gttacgcgcc tcctcaggac ttcgggcggc
     1321 gtctggaaac atgacaatcc acgcaccccg gaataattct catgctgagg agggcccgaa
     1381 gggcccgtct cgaagcatga gtccgacact cagatcgccg gcttcgagcg gtgccgccgc
     1441 gcaagtacca ccagcgcttc aaaattgccg ctggcaaggg cctccttctt tgctcggctc
     1501 cagcccttga tttgacgctc cgctgacaca gcgtctgtta ttcggtcgaa gtactcggcg
     1561 tagaagagtg tcaccggccg gcgcgtggcg gtgtagccac caaacgagcc ggtattgtgc
     1621 tgattgatac gatctcaagt gagccgcgcg tggagcctgt atagaaacta ccatcgcggc
     1681 agcgcaaaat gtagaactat gcacccatgt cccaaaaaat cctcatgctg aggagggccc
     1741 gaagggcccg tctcgaagca tgccatattg gtaacatctt aaacctcacc ctccgagatg
     1801 gcgcgttccg cgcctcctca ggatgaggac ttcaaccggt aaggaaccat ccttgtcttc
     1861 gcaaaacgca acagcaacgg agctgcttcg cgcgctcgtg gccttcgaca ccacgagtgc
     1921 caagtcgaat ctcgcgctga tcgccttcgt cgagaactat ctcaaaggcc acggcatcgc
     1981 cgccacgcgc gtgtcctctc ccgacggcgc aaaggccgat ctgttcgcca ccatcgggcc
     2041 gaagggcgac agtggcatcg ggctctccgg tcacagcgat tgcgtgcccg tcgaaggcca
     2101 gcgctggacc tccgacccct tcacgctcac cgagcgcgga agcaagctat acggccgcgg
     2161 gagctgcgac atgaagggct tcatcgcctg cgcgctcgcc gccgtgccct ccttcaaagc
     2221 ccgggcgttg aaagagccaa tccacatcat cgtttcctat gacgaggagg tcggatgcac
     2281 cggggtgagg ccgctcatcg cccgtctcgg caaggacctg ccgaagccgc gcgcggtcat
     2341 cgtcggcgag ccgaccggca tggcggtcat cgacgcgcat aagcgcatcg atgcctaccg
     2401 cacgcacgtg acgggccgcg aggcgcattc gagcatgccc gagcttggcg tcaacgccat
     2461 cgccgccgcg gccgcgctga tcggggaatt ggatcggatc ggcggcgaga tcgccaagcg
     2521 ggagaaggac aagcgcttcg agccgcccta ctcgacggtg tgcgtcggta ccatcgaagg
     2581 cggaacggcg cacaacatcg tgccgaagac ttgcgcgttt caatggcagg tacggagcct
     2641 gccctcggtt gaacctggcg aagtgccgcg tgggcttgcc gccttcgccg agacgctctt
     2701 gccgaagatg cggcaggtga cgaaggaggc cgcgatcgac accgagggcg aaggttcggt
     2761 gccggctttc gtggctgcag ggggatcgga agcggtggcg ctcgcgctcg ccctcactgg
     2821 cgcggaccac accgaggccg tgtcctacac gaccgaagca gggctgttcg aagaggcgag
     2881 cgttccggcg gtgatctgcg gccccggcaa tatcgcgcag gcccatgccg ccgacgagta
     2941 cgtcacgatc gcgcagctcg aagcgtgcct cgcctttctc gatggcctcg ccgcgaggct
     3001 cagcacctga cgctcaagcg tcggcaaagg tcgcgggatc gaagcggtag cgcctgttgc
     3061 agaactcgca cgtgacggag acttcgccgt ccacgaccat ctcggcaaga tcctcggcgg
     3121 agaagcgcct gagcagttcg tcgacccgcg cctccgagca gctgcagtag cctttcagcg
     3181 ggacagcgcg ataggcacgc acctgctcct cgtggaacag acggtagagc agccggtcgg
     3241 gcggcagcgt gggatcgagc agctcgtgat cctcgaccgt ttcggcgagc gccttggcgc
     3301 gcgtccagtc ctcttgcgtg aagg
//
LOCUS       DASUQP010000037         5556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_16040,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000037 DASUQP010000000
VERSION     DASUQP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16040"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFY74_01775"
     CDS             <1..288
                     /locus_tag="VFY74_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930467.1"
                     /translation="LHHFKPPQIVEIFARCAKLAPVFIALEPRRSMLPLIGSRLIGLL
                     GCNYVSRHDATASVRAGFRDRELSALWPDPKAWILREGRVALSHVFAAQKC"
     gene            282..1511
                     /locus_tag="VFY74_01780"
     CDS             282..1511
                     /locus_tag="VFY74_01780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5930468.1"
                     /translation="MLRIAPRKRYDAAVIGGGPAGSATALILARAGWSVAIVEKSEFP
                     RPKVCGEFISASTFPLFAAFGLLDDVCGLAGPEVRRVGMFAGKIRSTAHMPEVSGPFG
                     EWGRALGREHLDLLMLEAAASAGADVWQPCRVTGVQPLDQGWRCDISHDRRNTSLFAR
                     TLIAANGSWEKNPWLPECDAPHRDSDLIAFKAHFAGADLAPDLMPLLVFPGGYGGMVW
                     SDAGRVSLSCCISRKVLNRCRRNEERAGDAVLRHLSQSCEGVASALGRAKLRDAWHAA
                     GPIRTGIRPAFADGIFRVGNAAGEAHPIIAEGITMAIQSAWLLGRALIAEQDSLTKAS
                     ALAEAGRAYSARWRAHFAGRIHAAAAFAHTLMRPGSAALAVAFPKRFPALLTFCAALS
                     GKTKQISIASPLRVSSL"
     gene            1525..2001
                     /locus_tag="VFY74_01785"
     CDS             1525..2001
                     /locus_tag="VFY74_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEX5930469.1"
                     /translation="MDQLLTASTLLAALGSALIAGAFFAFSSFVLAALARLPAPAGIA
                     AMQSIIAAIKTPFFLAVFFGTAALAALLGIAAPLRWSEPGALYLLIGSLLYLNGPFGV
                     TLFKNLPLNNKLAAMKSESAEGARFWDEFRSAWGLWNHVRWIGALGASAAFILALA"
     gene            complement(1982..2629)
                     /locus_tag="VFY74_01790"
     CDS             complement(1982..2629)
                     /locus_tag="VFY74_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX5930470.1"
                     /translation="MKSVAIAAVLVMGLLFSPLDRAAAEPEPPVVVVLGDSLTAGFGL
                     PQDQAFPAQLEAALKTRGIAATVVNAGVSGDTAAAGLARLDWALPDNARAVIIELGAN
                     DALQGLPPEGTKTALQSIIEKIQAKGLPILLAGMEAPRNLGKDYVDAFRALYADLAAR
                     YDLILYPFFLESAALNDDLMQRDGLHPNARGVAAIVEDILPKVEELLARIKPAPG"
     gene            2700..3419
                     /locus_tag="VFY74_01795"
     CDS             2700..3419
                     /locus_tag="VFY74_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5930471.1"
                     /translation="MDHQLKQRQSPAIKLEGIEVTLPSRAGPVAILRGIDLSVTAGDA
                     IAVVGPSGSGKSTLLMVIAGLERATSGSVEVMGTGYASLDEDGLALLRAANIGIVFQS
                     FHLVPTMTALENVALPLEFLDRDDAFETARTALADVGLSHRQSHFPGQLSGGEQQRVA
                     IARALSPRPSLILADEPTGNLDLATGAEVMDLLFGLKARTGATLILITHDRSLAKRCD
                     RIVSLADGRIVSGAKRKVAAS"
     gene            3416..>5556
                     /locus_tag="VFY74_01800"
     CDS             3416..>5556
                     /locus_tag="VFY74_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5930472.1"
                     /translation="MTASSAGGANAASRFNPKLAFAMALRELRSGAGGLAVFVLCIAL
                     GVAAVAAIGSLAASFDEALARQGRLLIGGDLSFELIHRQASPGERAALEALGRVSESA
                     SFRAMARSPDGKSALVEVKAVDDAYPLYGDVSILDSDTTDPVWREPSVVLAERTLLDR
                     LGLSVGSTLAIGEATVTIGGILGEQPDRLADRLAYGPKLLMSRETLAKTGLVQPGSLI
                     RWTYRVKLPGPRASDKGALASARSEIESKFPQAGFAIRDWTDPAPSLRREADRFTQFI
                     NFVGLTALLLGGIGVGNAIQSYMTKKREIIATFKCLGAASRLVLAVYLIQALLLTAVG
                     IAMGLLIGALTPAILSAFYGDTLPITLALEPHPLPLLTASLAGLLTMILFVLWPLGRA
                     ASVSPAVLMRSHLTEEPKHSAWPYAAGAAIAGVALFALAIAASEERAVTASISAGIVA
                     AFLLLTGFGLLLQRYAARFRRTRPPSLALALTSIAGPNSLARSIAVSLGLGLGLLVAV
                     ALIHRSLYAEIANNIAIDAPAYYFLDVEAKDLDAFQQLVRKIEPGAKLDDAPMLRGRI
                     VALKGVPAEKIEAPPDMRWVLAGDRGLTYTDTVPGASTLVAGEWWPKGYSGPPLVSFD
                     AELAEGLGLKLGDPITVNILGRNVEATIASLRKIDWESLAINFVMVFSPNALQGAPHR
                     VITTLEFPKGADEARESKVIQALSARFPLVTA"
BASE COUNT          848 a         1956 c         1724 g         1028 t
ORIGIN      
        1 ctccatcact tcaagcctcc gcagattgtg gaaattttcg ctcgatgcgc caagctcgcc
       61 cctgtcttca tcgctctcga accgcgccgt tccatgcttc cgctgatcgg cagccggctc
      121 ataggcctcc tcggctgcaa ctatgtcagc aggcacgacg cgacggcgag cgtgcgagcc
      181 ggcttccgcg accgcgaact gtcggcgttg tggccggacc caaaggcctg gattcttcgt
      241 gagggtcgcg tcgcactctc gcacgtcttc gccgcgcaaa aatgctgagg atcgcgccaa
      301 ggaaacgcta cgacgcggcc gtcatcggtg gcgggccggc gggctccgcc acggcgctca
      361 tcctggcccg cgccggatgg tcggtggcga tcgtcgagaa aagcgaattt ccccggccga
      421 aagtctgtgg cgaattcatc tcggcgagca cgttcccgct gttcgcggcg tttggcctgc
      481 tcgacgatgt gtgcgggctc gccggccctg aggtgcggcg cgtcggcatg ttcgccggca
      541 agatcagaag caccgcccac atgcctgagg tgtcaggacc tttcggcgaa tgggggcggg
      601 cactcgggcg cgagcacctc gatctcctca tgctggaagc cgcagcgagc gccggcgccg
      661 atgtttggca accgtgtcgc gtcaccggcg tccaaccgct ggaccagggc tggcgatgcg
      721 acatcagtca cgacaggaga aacacgtccc tctttgcgcg gacgctcatc gcggccaacg
      781 gatcatggga gaagaatccc tggctgcccg aatgcgacgc gccccacagg gattccgatc
      841 tcatcgcgtt caaggcacat ttcgcaggcg cggaccttgc gcccgacctc atgccgctcc
      901 tcgtctttcc cggcggttac ggcgggatgg tctggagcga tgcagggcgc gtcagtctct
      961 cctgctgcat cagccgcaaa gttttgaacc gatgccgcag aaacgaagag cgcgccggag
     1021 acgcggtact gcggcatctc agtcagtctt gcgagggtgt ggcgagcgcg ctcggccgcg
     1081 cgaagctcag agatgcatgg cacgctgcgg gcccgatcag gaccgggatc cggcccgcgt
     1141 tcgccgatgg aatcttccgt gtcggcaacg ctgccggcga ggcccatccg atcatcgccg
     1201 aagggatcac catggcgata caatcggcct ggcttctcgg tcgcgcactc atcgccgaac
     1261 aggattcgct tacgaaggct tcggctcttg ccgaggccgg tcgcgcctat tcggcgcgct
     1321 ggcgcgcaca tttcgccggc cgcatccacg ctgcagccgc ctttgcgcat acgctgatgc
     1381 gcccgggaag cgccgcgctg gcggtcgcct ttccgaagcg tttcccggca ctgctaacct
     1441 tctgcgctgc gctcagcggc aagaccaagc agatttcgat tgcaagcccg cttcgcgtct
     1501 cgtctctatg agcgaggacc taggatggac cagctgctca ccgcatcgac actgcttgcc
     1561 gcgctcggct ctgcgctgat cgcgggcgcc ttcttcgcct tctcgtcttt cgtattggcc
     1621 gcgcttgcgc gcttgccggc tccggccggc attgccgcca tgcaatcgat tatcgccgcc
     1681 atcaagaccc cgttctttct tgccgtgttc ttcgggactg cggcgctcgc ggccctcttg
     1741 ggcatcgccg cgcccctgcg ctggtcggag cccggcgcgc tttatctcct gatcgggagc
     1801 ctgctctatc tgaatggccc gttcggcgtg acgctgttca agaacctgcc gctcaacaac
     1861 aagctcgccg ccatgaagtc cgagagcgcg gagggcgcgc gattctggga cgaattccgc
     1921 tccgcgtggg gcctgtggaa tcacgtgcgc tggatcggcg cgcttggcgc ctcggccgcc
     1981 ttcatcctgg cgctggcttg atccgagcga gaagctcttc gaccttcggc agaatatcct
     2041 cgacaatcgc cgccacgccc ctggcattgg gatggaggcc gtcgcgctgc atcaggtcat
     2101 cgttgagagc cgcgctctcg aggaagaacg ggtagaggat caggtcgtag cgggccgcga
     2161 gatcggcata caaggctctg aatgcatcga catagtcctt gcccaggttg cgcggcgctt
     2221 ccatgccggc gagcaggatc ggcagcccct tcgcctgtat tttttcgatg atggactgaa
     2281 gcgccgtctt cgtgccttcg ggcggaagcc cttgcagcgc gtcgttggcg ccaagctcga
     2341 tgatgacggc acgcgcattg tctggaagcg cccagtcgag ccgcgcgagc ccggccgccg
     2401 cggtatcgcc ggacacgccg gcattgacga cggtcgccgc aatgccgcgc gtcttcagcg
     2461 ccgcctcgag ctgcgcaggg aaggcctgat cctgcggcaa tccgaagcct gccgtcagac
     2521 tgtcgcccag cacaacgacc accgggggct caggctcggc cgcggcgcga tcaagcgggg
     2581 agaagagcaa gcccatgacc aacaccgctg cgatcgcgac agacttcatc ttttcgacct
     2641 ctttgccctt ctctattgcc gatcctcatg ccatctgtca gggactcttt gccccgccca
     2701 tggatcacca actgaaacag cgccaaagcc cggcaatcaa gctcgaaggc atagaggtga
     2761 cgttgccctc gcgcgccggg ccggtcgcga tcctgcgggg catcgacctg agcgtaacgg
     2821 cgggggatgc cattgccgtg gtcggccctt ccggctccgg caagtcgacg ctgctcatgg
     2881 tgatcgctgg ccttgagcgc gcaacaagcg gcagtgtcga ggtcatgggc accggctacg
     2941 cgagcctcga cgaagacggc ctcgctttgc tccgtgccgc caatatcggc atcgtgtttc
     3001 aatccttcca tctcgtcccc acgatgacgg cgctcgagaa cgtggcgctg ccgctcgaat
     3061 ttctcgaccg tgacgatgcg ttcgaaaccg cgcggacagc ccttgccgac gtggggctct
     3121 cccatcgcca atcgcatttc cccggccagc tttccggcgg cgagcagcag agagtggcga
     3181 tcgcccgcgc gctcagcccg cgcccctccc tcattctcgc cgacgagccc accggcaatc
     3241 tcgatctcgc caccggcgcc gaggtgatgg atctcctgtt cggtctcaaa gcgcgcaccg
     3301 gagcgacgct cattctcatc acccatgacc gctcgctcgc caagcgctgc gaccggattg
     3361 taagccttgc cgatggccgc atcgtgagcg gcgccaagcg caaggtcgcc gcatcatgac
     3421 cgcctcgagc gcaggcggag ccaacgcggc ctcccgcttc aatccgaagc tcgcctttgc
     3481 catggcgctc cgcgagctgc gctcgggcgc cggcggtctc gccgtgttcg tgctctgcat
     3541 cgctcttggc gtcgccgccg tggcggcaat cggttcgctc gcggcgtcgt tcgacgaggc
     3601 gctcgcgcgg cagggccggc tcctgatcgg gggcgatctc tccttcgagc tgatccaccg
     3661 ccaagcgagc cctggggaac gcgccgcgct cgaggcgctc ggtcgggtaa gcgaatcggc
     3721 gagcttccgc gccatggcgc gcagcccgga tggcaagagc gcgctggtcg aggtcaaggc
     3781 cgtcgatgac gcctatccgc tctatggcga cgtctcgatt ctcgattccg atacgaccga
     3841 tcccgtttgg cgcgagcctt cggtcgttct ggccgagcgg actctcctcg accgcctcgg
     3901 tctttcggtt ggcagcacgc ttgccatcgg cgaggcaacg gtcacgatcg gcggcatcct
     3961 gggcgaacag cccgaccggc tggccgaccg cctcgcctat ggaccgaagc tcctgatgtc
     4021 gcgcgagacg ctcgcgaaga cgggcctcgt gcagcccggc agtctcatcc gctggaccta
     4081 tcgcgtgaag cttcctggcc cgcgcgcgag cgacaaaggg gccttggcga gcgcccgcag
     4141 cgagatcgaa agcaagtttc cgcaagccgg cttcgccatc cgcgattgga ccgaccccgc
     4201 gccgtctctc cgccgcgagg ccgaccgctt cacccaattc atcaacttcg tcgggctcac
     4261 cgcacttctt ctcggcggca tcggcgtcgg caacgcgatc cagagctaca tgaccaagaa
     4321 gcgcgagatc atcgccacgt tcaaatgcct cggcgcggca agccggctcg ttctcgccgt
     4381 ctatctgatc caggcgcttc tcctcaccgc tgtcggcatc gccatggggc ttctcatcgg
     4441 cgcgctcacg cccgccatcc tctcggcctt ctatggcgat acgctgccca tcacgcttgc
     4501 gcttgagccg caccccttgc cgctgctcac cgcttctctt gccgggctcc tgaccatgat
     4561 cttgttcgtg ctgtggccgc tcggccgcgc cgcctccgtt tcgcccgccg tgctgatgcg
     4621 ctcccatctc accgaggagc cgaagcattc cgcgtggcct tatgcggcgg gggcggcaat
     4681 tgcgggcgtc gccttgttcg cgctcgccat cgccgcttcc gaggaacgcg ccgttaccgc
     4741 ctctatctcg gccggcatcg tcgcggcctt cctgctgctc accggcttcg gcctgctgct
     4801 gcagcgttac gcggcgcggt tccggcgcac gcggccgcct tcccttgcgc tcgcgctcac
     4861 gagcatcgcc ggaccgaatt cgctcgcccg ctccattgcc gtctcgctcg gcctcggcct
     4921 tggccttctc gtggcggtgg cgctgatcca ccgctcgctt tacgccgaga tcgccaacaa
     4981 catcgcgatc gacgctccgg cctattattt cctcgacgtc gaggccaagg acctcgacgc
     5041 cttccagcaa ctggtgcgga agatcgagcc cggcgccaag ctcgacgatg cgccgatgct
     5101 cagaggccgc atcgtggcgc tgaaaggcgt tccggccgag aagatcgagg cgccccccga
     5161 catgcgctgg gtcctcgcgg gcgaccgcgg gttgacctac accgacacgg tgccgggcgc
     5221 ttcgacgctc gttgcgggcg agtggtggcc caagggctat tcgggaccgc cgctcgtctc
     5281 gttcgacgcc gagcttgcgg aagggctggg cttgaagctc ggcgatccga tcaccgtgaa
     5341 cattctcggc cgcaatgtcg aggccaccat cgcgagcctg cgcaagatcg actgggagtc
     5401 gctcgccatc aacttcgtca tggtgttctc gccgaacgcg ctgcagggcg cgccgcatcg
     5461 cgtgatcacg acgctcgaat tccccaaggg cgccgacgaa gcgcgcgaaa gcaaagtcat
     5521 acaggcgctc tccgcgcgct tcccgctcgt caccgc
//
LOCUS       DASUQP010000038         5109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_697935,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000038 DASUQP010000000
VERSION     DASUQP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697935"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFY74_01805"
     CDS             <1..501
                     /locus_tag="VFY74_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTE family protein rssA"
                     /protein_id="HEX5930473.1"
                     /translation="GIFRPVQINGRWLIDGALVNPIPVSVCRALGARIVIAVNLSSDP
                     FGRGGVIHDYQAFAEPFAAAEDTELPGSNGRAAVHLLHRQIFGRSDGAPGISAVMMDA
                     INITQDRIARSRLAGDPPDIIIGPKTGGIGLFEFHRACEAIELGAKAAEKQIDEIKLA
                     VRSLAS"
     gene            complement(506..937)
                     /locus_tag="VFY74_01810"
     CDS             complement(506..937)
                     /locus_tag="VFY74_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5930474.1"
                     /translation="MNVAAILKQKGRGVFTTTPDTSLLDIAKLLGLHRVGCIVITGPD
                     GKVIGIVSERDIVREISRAGSRVLKEPVERCMTKSVVTCREADTIDRLMAEMTAHRFR
                     HMPVVERGRLIGLVSIGDVVRMRIAEAEMEAAAMREYIATG"
     gene            complement(1034..1762)
                     /locus_tag="VFY74_01815"
     CDS             complement(1034..1762)
                     /locus_tag="VFY74_01815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5930475.1"
                     /translation="MTRAEPALNVPRVVTGVAALLTGVQAVKSFLPDETKITLLLALA
                     FIPARYSGAALDLPGGYVTAVTSFVTYMMVHAGWVHLAVNLMWMLAFGSALARRMTPL
                     AFLSFSVLCGIAGAFTHLLFHWGDLTPVIGASAATSGQMAGALRFFFNAKPRAGQRAP
                     DFGGAPLMSLGQTLSDRRMLIVLGLWIALNAYFGIAAVKIGGEEGSIAWEAHIGGFLC
                     GLLIFGAFDKGKYGGKRSEAPQDA"
     gene            2089..2709
                     /locus_tag="VFY74_01820"
     CDS             2089..2709
                     /locus_tag="VFY74_01820"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEX5930476.1"
                     /translation="MFVSSLSADATNDHAISLVPAPQVQKIAFMSAFGDTLPPIGYVT
                     FCRDNQSECRPARRFADRIQLTAAKLEEIEKVNDSVNAAVAPVTDFELYGKPEMWAYP
                     SGNKGDCEDYVLLKRRLLVSRGFPESTLLITVVRDENNEGHAVLTVRTDRGDLVLDNK
                     RRQIMRWAETPYTFVKRQSERNPLVWISLLPPETAPQTAVSASNSN"
     gene            complement(2862..3074)
                     /locus_tag="VFY74_01825"
     CDS             complement(2862..3074)
                     /locus_tag="VFY74_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEX5930477.1"
                     /translation="MTRDEILKLEHYLKKVFRLPAIQVRQRPRKDDSAEVFVDEEFIG
                     VIFRDDDEGEVAYQFQMAILDYDLKE"
     gene            complement(3113..3949)
                     /gene="cysE"
                     /locus_tag="VFY74_01830"
     CDS             complement(3113..3949)
                     /gene="cysE"
                     /locus_tag="VFY74_01830"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX5930478.1"
                     /translation="MSKPMAAKEQHIKSYDPIWSQVRRQAEEISAAEPALGGFIYATV
                     QDHDRLEDAVCHRLARRLQHSTLDTGLLHKLFNEVIAADPSLGDAFRADLMAVAKRDP
                     ACSRVIEPLLYFKGFHALETYRIAHSLWKAGRKDFALYLQSLTSRFLQVDIHPAAVIG
                     RGIMFDHATGIVIGETAVIGDNCSLLHGTTLGGTGNEKGDRHPKIGRGVMVGAGAKIL
                     GNIKVGDCARIAAGSVVLNDVASRRTVAGVPAREIGFAGCEEPALAMDQMVSGDDETS
                     HA"
     gene            complement(4010..4762)
                     /locus_tag="VFY74_01835"
     CDS             complement(4010..4762)
                     /locus_tag="VFY74_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5930479.1"
                     /translation="MQSFESDGVRIAFVDEGKGDPILLIHGFASNVARNWRDAHWLSA
                     LAAASRRAVAYDNRGHGQSEKLYDPSRYGAPTMAEDALRLLDHLDIGRADVLGYSMGA
                     RIAVFLALAHPERVRSLILAGTGINLVRGMVGTGPIARALEAPRIEDVTNDTARSFRA
                     FAEKTGSDLKALAACMRGPREKIGPEDLARIAVPTLIATGSEDVIAGSGKELKDLIPG
                     AQLLDIEGRDHMKAVGDPRFKQGVLDFLTGRA"
     gene            4839..5102
                     /locus_tag="VFY74_01840"
     CDS             4839..5102
                     /locus_tag="VFY74_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX5930480.1"
                     /translation="MAQPLVPHFVNDKGVGRIRIGVKEFQCMGATPPFDHPHVYLDMG
                     DVAEIVCPYCSTVFEHDHAIKANESDPPSCVFAATAPPGAPAA"
BASE COUNT          965 a         1674 c         1530 g          940 t
ORIGIN      
        1 ggcatcttcc gcccggtcca gatcaacggc cgctggctga tcgacggggc cctcgtcaac
       61 ccgatcccgg tttcggtgtg ccgcgcgctt ggcgcccgca tcgtcatcgc cgtcaacttg
      121 agcagcgacc ccttcggcag aggcggcgtc atccacgatt accaggcgtt cgccgaaccc
      181 tttgcggcgg cggaggatac cgagcttccc ggcagcaatg gccgcgccgc ggtgcatctg
      241 ctgcaccggc agattttcgg caggagcgac ggcgcgcccg gcatctctgc ggtgatgatg
      301 gacgccatca atatcacgca ggatcgcatc gcgcggtcgc ggcttgccgg cgacccgccc
      361 gatatcatca tcgggcccaa gaccgggggc atcgggctgt tcgagttcca tcgcgcgtgc
      421 gaggcgatcg aactcggcgc caaggcagcc gagaagcaga tcgacgagat caagctcgcc
      481 gtacggtcgc tcgcgtcgtg agcgtctagc cggtggcgat gtattcgcgc atcgccgccg
      541 cttccatctc cgcctcggcg atccgcatcc gcaccacgtc gccaatcgac acaagcccta
      601 tcaaccggcc tctttcgacc acgggcatgt ggcggaatcg atgcgccgtc atctcggcca
      661 tcagcctgtc gatggtgtcg gcctcgcggc acgtcaccac cgatttggtc atgcaccgct
      721 cgaccggctc cttgagcact ctcgatcccg cccgcgagat ctcgcgcacg atatcgcgct
      781 cggagacgat accgatgacc ttgccgtcag ggccggtgat cacaatgcag ccgacgcggt
      841 gcaggccgag cagcttggca atgtcaagca gcgacgtgtc gggcgtcgtc gtgaacaccc
      901 cccgtccctt ctgcttcagg attgcagcga cattcatagg cgtgtcctcc agccccggaa
      961 ctttccgccc ggtgactgcg acattctgtc ccggacctga cgaacgcgca agagcgagca
     1021 aaagcggagg actttaggcg tcttgaggtg cttccgaacg ctttccccca tacttccctt
     1081 tgtcgaaggc gccaaaaatc agtaacccgc agagaaaccc accgatatgg gcctcccagg
     1141 cgatgcttcc ctcctcgccg ccgatcttga cggcggcaat gccgaaatag gcgttaagcg
     1201 cgatccagag accgagcacg atcagcatac gcctatcgct cagcgtttga ccgaggctca
     1261 tgagcggcgc gccgccaaaa tccggtgccc gctgtcccgc ccgaggcttg gcgttgaaga
     1321 agaatcttag cgcgcccgcc atctgaccgg aggtcgccgc cgaggccccg atgaccggcg
     1381 tcaggtcgcc ccagtggaac agaagatggg tgaaggcccc tgcgatcccg cacaacaccg
     1441 agaacgagag aaacgcgaga ggggtcatgc ggcgggccag ggcgctgccg aaggcaagca
     1501 tccacatgag attgaccgcg agatgcaccc atccggcatg caccatcatg taggtcacga
     1561 atgacgtgac cgcggtgaca tatcccccgg gaagatcgag cgcggcgccg ctatagcgcg
     1621 ccggaatgaa ggcaagcgcg agaagcagcg tgatcttggt ttcgtcgggc aagaaactct
     1681 taaccgcttg cacgccggtt aacagcgccg caacgcccgt cacgacgcgc gggacattga
     1741 gcgcgggttc agcccgggtc atgaccggac cgagcggaat ggtgtggatt caatcgcggc
     1801 aacgcttccg tccagcaatc gcatgccccc atgcccttgc gcagtccaaa cgtcgtcggt
     1861 ccaagagcat tggaaaggcg ccgtacgcaa gagctccccc gccgcattgc cgaaatttta
     1921 acgatgaccg gacgttcagg tttgagtagg tgtcgctaaa aaactcctac caatactcct
     1981 ccaaaacaat cacgcgtttt atcgggatct caaaatctcc ctgcgatagt gcgccccaat
     2041 aatacggggg gtgcaatgaa acagtttatc gtaggctccg ccttcttgat gtttgtctcg
     2101 tccttgtcgg ccgacgcgac caacgatcac gcgatcagtc tggttccggc gccgcaggtg
     2161 cagaagatcg ccttcatgtc ggctttcggc gatacgctgc cgccgatcgg ctatgtcacg
     2221 ttttgccgtg acaatcagtc cgagtgccgc cctgcgcgcc gcttcgccga ccgcatccag
     2281 ctcaccgcgg cgaaactcga agagatcgag aaggtgaacg acagcgtgaa cgcggcggtc
     2341 gctccggtca ccgatttcga actctacggg aagcccgaaa tgtgggccta tcccagcggc
     2401 aataagggcg attgcgagga ctatgtgctg ctcaagcgcc ggctcctcgt ttcgcgcggg
     2461 tttcccgaga gcacgctcct catcaccgtc gtccgcgacg agaacaacga gggtcatgcg
     2521 gtgctgacgg ttcgtaccga tcgcggcgat ctcgtcctcg ataataagcg ccggcagatc
     2581 atgcgttggg ccgagacgcc ctacaccttc gtcaagcggc agtccgagcg gaatccgctg
     2641 gtgtggatct cgctcttgcc gcccgagacg gcgccgcaga cggccgtctc cgcttcgaac
     2701 agcaattagc tgaaggactt cgaccccaaa tccttgggga catcagataa ggcccggtga
     2761 ccatccccca cccatccccg acccaaccgg gcctttactc gagccggcct cgtccaagag
     2821 gccggctctt cttttttctg ctttagggga aggctgcggc gctactcttt gaggtcgtaa
     2881 tcgagaatcg ccatctggaa ctgataggcg acctcgccct cgtcgtcgtc gcggaagatc
     2941 acgccgatga actcctcatc gacaaagacc tcggcggagt cgtccttgcg cgggcgttgc
     3001 cgtacctgga tggcaggcag ccggaacacc ttcttgagat aatgttcgag cttaaggatt
     3061 tcgtcccgcg tcaccgctcg ctccccaagc tcgtttcact tgccgcgcgt gctcaggcgt
     3121 ggctcgtttc gtcatcgcca gagaccatct gatccatggc aagggcaggt tcctcgcatc
     3181 cggcgaagcc gatttcgcgc gccggcaccc ctgccacggt cctcctcgat gcgacgtcgt
     3241 tcagcacgac gctgccggcg gcaattcggg cgcagtcgcc gactttgata ttgccgagga
     3301 tcttggcgcc ggctccgacc atgacgccgc gtccgatctt gggatggcgg tcgcccttct
     3361 cgttgccggt gccgccgagg gtcgtgccgt ggagcagcga gcaattgtcg ccgatcaccg
     3421 ccgtctcgcc gatgacgatg cccgtggcgt ggtcgaacat gatgcctctg ccgatgaccg
     3481 cggccggatg gatgtcgacc tgcaggaaac gcgaggtgag gctctgcaga tagagggcga
     3541 agtccttgcg ccccgccttc cacagcgaat gcgcgatgcg ataggtctca agcgcgtgaa
     3601 agcccttgaa atagagcaac ggctcgatca cgcggctgca cgccgggtcg cgcttggcga
     3661 cggccatcaa gtcggcgcga aaggcatcgc cgagcgacgg atcggccgcg atcacctcat
     3721 tgaataattt gtgcaagagg ccggtatcga gggtcgagtg ctggagccgc cgcgcaagcc
     3781 ggtggcagac ggcatcctcg agccggtcat ggtcctgcac tgtcgcatag atgaatccgc
     3841 cgagagcggg ttcggccgcg ctgatttcct cggcctggcg ccgcacctgg ctccagatcg
     3901 gatcgtaact cttgatgtgc tgctctttgg cagccatagg ctttgacatg ggaacctcac
     3961 cgccccgttt ggaaccgggg ccgatcgaaa acaccgaacg cgtactcaac taagcccgcc
     4021 cggttaaaaa gtcgagcacg ccttgcttga agcgggggtc tccaaccgcc ttcatatgat
     4081 ctcgtccttc gatatcaagg agttgggcgc cagggatcaa gtccttcagt tcctttcccg
     4141 aacctgcgat cacgtcttcg ctcccggtcg caatgagggt cggcacggcg atgcgggcga
     4201 ggtcttcggg gccaatcttc tcccgcggcc cacgcataca ggcggcgagc gccttgagat
     4261 cgctccccgt cttctcggca aaggctctga agctgcgcgc cgtgtcgttg gtcacgtcct
     4321 cgatgcgcgg cgcttcgagc gcgcgcgcga tcggaccggt tcccaccatg ccgcgcacca
     4381 gattgatgcc ggtgcccgcc aagatcaggc tgcgcacacg ctcgggatgc gccagggcga
     4441 gaaacacggc gatacgcgcg cccatggaat accccaaaac atcggcgcgg ccgatgtcga
     4501 gatggtcgag cagcctcagc gcatcctctg ccatggtcgg cgcgccgtaa cggctcgggt
     4561 cgtagagctt ctcgctttgc ccgtgcccgc gattgtcgta ggcgacggcg cggcgcgacg
     4621 ccgccgcgag cgctgacagc caatgcgcgt cgcgccagtt ccttgcgaca ttggaggcaa
     4681 aaccgtggat gaggagaatg gggtctcctt tgccttcatc cacaaaggcg atcctaacgc
     4741 catcggattc gaagctctgc attcttgacc taccgctttg ccgccggggt cactatgctc
     4801 gcgaccaatt gcactgacga gacagaagag cagcacccat ggcgcaaccc ctcgtgcccc
     4861 atttcgtgaa cgacaagggc gtcggccgga tcaggattgg cgtcaaggag tttcaatgca
     4921 tgggggcaac accacccttc gatcacccgc atgtctatct cgacatgggt gacgtggccg
     4981 agatcgtctg cccctattgc tcgaccgtgt tcgagcacga tcacgcgatc aaggcaaacg
     5041 agagcgatcc gccctcttgc gtcttcgcgg cgaccgcccc gccgggggct ccggcggcct
     5101 aaaacagca
//
LOCUS       DASUQP010000039        13504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1924789, whole genome shotgun sequence.
ACCESSION   DASUQP010000039 DASUQP010000000
VERSION     DASUQP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13504
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924789"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /gene="mnmG"
                     /locus_tag="VFY74_01845"
     CDS             <1..1607
                     /gene="mnmG"
                     /locus_tag="VFY74_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX5930481.1"
                     /translation="AVQGPRAQADRKLYRKAMQAAIAATPGLEVIEAAVEDLTVEDGD
                     VTGIVTGDGRRFSARAVVLTTGTFLRGLIHIGERKIPAGRIGEAPALGLSTRLYALGL
                     SMGRLKTGTPPRLDGRTIRWDGLEVQNGDDPPRPFSFLTERIATPQVVCHITHTTAAT
                     HAIIARNLARAPMYSGAIESVGPRYCPSIEDKIVRFKERGSHQIFLEPEGLDDDTVYP
                     NGISTSLPEDVQHAFLATIPGLETARVIRPGYAIEYDYIDPRELTAGLEVKAVAGLFL
                     AGQINGTTGYEEAAGQGLIAGINAALSASGGDRRFSVSRADGYFGVMIDDLVTRGVTE
                     PYRMFTSRAEYRLTLRADNADQRLTPLGIDVGCVGAARGAVFAAKSKKLAEGAALLGS
                     LALTPDEAAKFGLAVNRDGRKRSAFELLSYANVDVARLAAIWPEIGKLDPGIAEQLAV
                     DARYAVYMRRQELDIAAFRKDEGTAIPGDFAFAALPGLSAELRQKLDRHRPANLGQAA
                     RLDGMTPAALMLLLAHLKKGAMAKTA"
     gene            1649..2305
                     /gene="rsmG"
                     /locus_tag="VFY74_01850"
     CDS             1649..2305
                     /gene="rsmG"
                     /locus_tag="VFY74_01850"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX5930482.1"
                     /translation="MAITGPEDFAEVFRVPRETIHRLVRYSDLLALWQKSKNLVAPST
                     LERLWSRHFADSAQLSGLAPKARLWLDLGSGAGFPGLVIAILEAGRPDFRMHLVESNR
                     KKCAFLGEVVRATKAPVDVHAMRIEDLAENAHSLRPDVVSARALAPLPHLLELAAPFF
                     GPETRGLFLKGREIETEIAEARRRFDYSCRLHPSLTATDSHIVEVTALRRRGEGAGAR
                     "
     gene            2326..3114
                     /locus_tag="VFY74_01855"
     CDS             2326..3114
                     /locus_tag="VFY74_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5930483.1"
                     /translation="MRVLALANQKGGVGKTTTAINLGTALAAVGERVLIIDLDPQGNA
                     STGLGVDERPLSTFDVLSGQAALSAARVPTHVPRLSLVPATIDLMSFERESGAGSGRH
                     YRLRDCLAAMSADETPELATTYVLIDCPPSLNLLTVNALAAADAVLVPLQCEFFALEG
                     LAQLLSTVEEIRVRLNPKLQIHGIVLTMYDQRTSLSDQVVDDVRRVLGDKVYATIIPR
                     NVRVAESPSHGKPVLLYDYRCSGSQAYIQLASEVIERERRMRAA"
     gene            3150..4007
                     /locus_tag="VFY74_01860"
     CDS             3150..4007
                     /locus_tag="VFY74_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5930484.1"
                     /translation="MAVGAQKTRLGRGLASLIGDRFDEAALGAEEDRRTVPLVALKPG
                     RFNPRRNFAEEQLEELAASIRERGLVQPIVVRPSSGDTYEIVAGERRWRAAQLADLHE
                     VPVIVRTLNDQEAVEIAIIENVQREDLNAIEEGEGYKLLIEGHDYTQEDLARIVGKSR
                     SHLANTLRLLKLPESVQDLVRSGALSAGHVRPLVGRTDAAALAERIVSRGLNVRQVEA
                     LTQDRGPAKTRRQGSKDADTRAAEAELHEALGLEVEIRKGKGEKGELRIRYATLDQLE
                     DLRHRLLRR"
     gene            complement(4154..4723)
                     /gene="pyrE"
                     /locus_tag="VFY74_01865"
     CDS             complement(4154..4723)
                     /gene="pyrE"
                     /locus_tag="VFY74_01865"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5930485.1"
                     /translation="MKERANTVELRTKLFALLKERAFRRGRVVLASGRESDFYFDMKP
                     AMLDPDGAALMAELILQEIQDVKADCVGGLEMGAVPLIAPVAMRSLDFDRRLHGFFVR
                     KAVKDHGTKKRIDGADIAGKHVIILEDVTTTGGSAMDAVKAVEEAGAKVALVLSILDR
                     GEGAADLYAKAGIPFASLFRAEEFLAVAR"
     gene            complement(4771..5784)
                     /gene="holA"
                     /locus_tag="VFY74_01870"
     CDS             complement(4771..5784)
                     /gene="holA"
                     /locus_tag="VFY74_01870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5930486.1"
                     /translation="MVVYKSPAVASFVKAPKADCRAVLVYGPDAGLVAERADALAQVF
                     ARRGRGATEIVRLDDRDLAEDPARIEVELRTVPMFAEQSVVRVAAGPKLDVPALKILL
                     AESFANVLIVEAGNLRPDSALRKAFEAHKSAAALPCYSDARDLSALIEAELGDAGLSI
                     DRETRDYLMTRLGADQALSRAEVVKLALYAQGAGAVNHDDVEAIVGDAAETALENFVY
                     ATSGGDAKAALSELQRLVASGTDKGAALSALGRHFIQLHRVAAAVASGRPPEEALRSL
                     KPPPRPRTREEAFLVRCRKWGAARLARALPLIQEAIKRSRLSPDLAGAFAERLVLSLT
                     SKV"
     gene            complement(5832..6353)
                     /locus_tag="VFY74_01875"
     CDS             complement(5832..6353)
                     /locus_tag="VFY74_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930487.1"
                     /translation="MWWRSLAVLASLLAGLGALALAGCGFQPLYGGSTAGGARISEVM
                     AGIDVAPIPGRVGQRLRNELIFANTGGGTAGPTRYRLDIAVKESVTDQLVQITGDATG
                     QVYQLDATFKLIDASKGVVVYQGKAISRAPYNRFQEIFANVRARYDAENRAARTVSES
                     IRTQVAAHLSNAA"
     gene            complement(6340..8949)
                     /gene="leuS"
                     /locus_tag="VFY74_01880"
     CDS             complement(6340..8949)
                     /gene="leuS"
                     /locus_tag="VFY74_01880"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5930488.1"
                     /translation="MAAERYDALRRERHWQAVWDERGLFRGSEDDRRPKYYVLEMFPY
                     PSGRIHMGHVRNYTMGDVVARYMHAKGHNVLHPMGWDAFGLPAENAAFERGIHPAKST
                     YDNIAVMRGQLKRLGLSIDWTREFATCDLDYYHQQQRLFLDLLKAGLVDRKTRKVNWD
                     PIDQTVLANEQVIDGRGWRSGALVEQREQPEWVFKITRYAEDLLRALDRLERWPDKVR
                     LMQANWIGRSEGLLVRFAVEGAPPEGHDVLEVFTTRPDTLFGASFLAIAPDHPLAKAI
                     AETNAEAGAFIDECHRHGTSAAEIETQEKKGFDTGLRVRHPLIEGATLPVFIANFIVM
                     EYGTGAIFGCPAHDQRDLEFARAEGLPVLPVVLPPGENPKRFAICEEAYTGDGLLINS
                     SFLDGLDVAAAKDAVAERLTKKKLKGKPVGDRQVQYRLRDWGISRQRYWGCPIPMIHC
                     PSCGIVPVPESDLPVKLPEDVSFDRPGNPLDHHPKWKHVSCPQCGGKAMRETDTMDTF
                     VDSSWYFVRFCAPKSHEPTDLAATRHWLPVDQYIGGVEHAILHLLYARFFTRAMHKTG
                     HVAMDEPFRGLFTQGMVTHETYKDVHGKWLLPEEVSRKDGEAVHAKTGEPVTVGAIES
                     MSKSKKNVVDPETIISAYGADTARWFMLSDTPPERDIEWSEAGVEGAHRFLQRMWRLV
                     REAAAKGAPPSGAKPAKFGPGAEALRRAAHRSLAAVTAAIEKLRFNTAVAQIHELANA
                     LSSGLQSAGPHPAPDQAYALREAAELLARIASPMIPHLAEECWAELGHETLLAEEPWP
                     APEKALLLEETVTIAVQVNGKRRDELTIARDAAKEDIEAAALKLENVVRAVGGRKIKK
                     VVVVPQRIVNVVA"
     gene            9044..9733
                     /locus_tag="VFY74_01885"
     CDS             9044..9733
                     /locus_tag="VFY74_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5930489.1"
                     /translation="MGSDPLDPVHVSERLALVRQEIALAAEAAGRAPHAVNLVAVTKT
                     VAAPVIAEAVAAGQRCFGENRVQEAHAKWPALKQSHADIELHLVGPLQTNKVREAVAL
                     FDVIETVDRPKLARALAEEMMRSGKRPRLFVQVNTGEEPQKAGVVPSEAETFIALCRD
                     TFGLTIAGLMCIPPLDEEPAMHFALLAKIAEKAGVTELSMGMSGDFARAISFGATYVR
                     IGTAIFGPRDE"
     gene            complement(9735..10268)
                     /locus_tag="VFY74_01890"
     CDS             complement(9735..10268)
                     /locus_tag="VFY74_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5930490.1"
                     /translation="MRVKRPRSVFVRPAPRFRWQARIVLGHGVRPAALGRTGIRSIKR
                     EGDGGTPLGRFAVRHVLYRADRVARPRTRLPVRAIRDDDGWCDDPADRNYNRLVRLPS
                     RRSAEGLKRADRLYDLVLVLGYNDVPRVKGKGSAIFVHLARPGFTPTDGCIALSRHDL
                     TMLLAHVRPRSAIVVSV"
     gene            10365..11051
                     /locus_tag="VFY74_01895"
     CDS             10365..11051
                     /locus_tag="VFY74_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5930491.1"
                     /translation="MSNARKILIIDDDDELRESLSEQLSLHEEFDTVLAATASEGVKQ
                     ARSGHIDLVLLDVGLPDLDGREACKLLRRGGFKGPVVMLTAQTSDADTILGLESGAND
                     YITKPFRLGVLLARIRAQLRQHEQSEDAVFAIGPYTFKPAAKLLVDGAGAKIRLTEKE
                     TSILKYLYRAGERVVTRDVLLHEVWGYNAGVTTHTLETHIYRLRQKIEKDPSQAELLI
                     TETGGYKLVP"
     gene            complement(11069..11884)
                     /locus_tag="VFY74_01900"
     CDS             complement(11069..11884)
                     /locus_tag="VFY74_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5930492.1"
                     /translation="MRFRLATWNINSVRLRLDLVRRFVRAHRPDFLCLQEIKCIEGHF
                     PARELRALGFPHIAVAGQKGYHGVATLSRIPFTLKERRDICGRGHARHLSIAIDGAGA
                     QPILLHNVYVPAGGDIPDPALNDRFRHKLEFLEALACWFEGMRGKRQSRMIMVGDLNV
                     APLPTDVWSHRQLLKVVSHTPAEVECLERVQASQGWVDVMRRHVPAEEKLFTWWSYRN
                     RDWRASNRGRRLDHVWTTPSLDGTARSMTVLDATRDWPRTSDHVPVLADFVIE"
     gene            complement(12127..12696)
                     /locus_tag="VFY74_01905"
     CDS             complement(12127..12696)
                     /locus_tag="VFY74_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX5930493.1"
                     /translation="MWAALAINAGMFLTEIVAGVIAGSVSLHADALDFLGDTFNYAIS
                     LAVLGLALSWRARAALLKGATMALLGLWVIGETVWHIVVGRVPEPLLMGGIGALALAA
                     NGLVLGLLYRFRESEANLRSAWICSRNDVLGNLAVLAAALGVFGTGTLWPDVIVAAAR
                     AALALQGSVAVIRQSLDELNGATLKASHP"
BASE COUNT         2419 a         4376 c         4442 g         2267 t
ORIGIN      
        1 ctgcggtcca gggcccccgc gcccaggccg atcgcaagct ctaccgcaag gccatgcagg
       61 cggcgatcgc cgccacgccg gggctagagg tcatcgaggc tgcggtcgag gatttgaccg
      121 tcgaagacgg cgatgtcacc ggcatcgtca ccggggacgg gaggcgcttc tcggcccgcg
      181 ccgtggtgct caccaccggc acgttcctgc gcggcctcat ccatatcggc gagcgtaaga
      241 tcccggccgg gcgcattggc gaggcccctg cccttggcct gtccacgcgg ctctatgccc
      301 tgggcctcag catgggccgg ctcaagaccg gcaccccgcc tcgcctcgac ggccgcacca
      361 ttcgttggga cgggctcgag gtgcaaaacg gcgacgaccc gccccggccc ttctcgttcc
      421 tcaccgaaag gatcgcgacg ccgcaagtcg tctgccatat cacccatacg accgcggcga
      481 cccacgccat catcgcgcgt aatctcgccc gcgcgccgat gtactctggc gccatcgaga
      541 gcgtcggccc gcgctactgc ccctccatcg aggacaagat cgtgcgcttc aaggagcgcg
      601 gcagccatca aatcttcctc gagccggaag gtctcgacga cgacaccgtc tatcccaacg
      661 gcatctcgac ctctctgccg gaggatgtgc agcacgcctt cttggcaacg atcccgggct
      721 tggaaacggc gcgggtgatc aggccgggct acgccatcga gtacgactac attgaccccc
      781 gggagctgac tgcggggctc gaggtcaagg ccgtcgcggg cctgttcttg gcgggccaga
      841 tcaacggcac gaccggttac gaggaggccg cggggcaggg gctcattgcc gggatcaacg
      901 cagccctttc cgcctctggc ggcgatcggc gcttcagcgt gagccgcgcc gacggctatt
      961 tcggcgtgat gatcgacgac ctcgtgaccc gcggcgtcac cgagccgtac cgcatgttca
     1021 cgagccgcgc cgagtaccgg cttacgctcc gcgccgacaa tgccgatcag cgcttgacgc
     1081 cgctcggcat cgatgtcggc tgcgtcggcg ccgcgcgcgg cgctgtcttt gccgccaagt
     1141 cgaagaagct cgctgaaggc gcggcccttc tcgggtcgct cgcgctcacg cccgacgagg
     1201 ccgcaaagtt cggcctcgcc gtcaatcgcg atggacgcaa gcgcagcgcc ttcgagctgt
     1261 tgagctacgc gaacgtcgat gtcgcgcgcc tcgcggcgat ctggcccgag atcggaaagc
     1321 tcgatccggg catcgccgag caactggctg tcgatgcacg ctacgccgtc tacatgaggc
     1381 ggcaggagct cgacatcgcg gcgttccgca aggacgaggg taccgcaatc ccgggcgact
     1441 tcgccttcgc ggccctgccc gggctctccg ccgagctgcg ccagaagctc gatcgccacc
     1501 ggcccgcaaa tctcggccag gccgcgaggc tcgacggcat gacccccgcc gccctcatgc
     1561 tgctcctggc ccatctaaaa aaaggcgcga tggcgaaaac cgcctgagcg ttgagggcga
     1621 gggcacgatg cccggtgaag agcgagcctt ggcgatcacg gggcccgagg atttcgccga
     1681 agtctttaga gttccacgtg aaacaatcca caggctggtc cggtactcgg atctcctcgc
     1741 gctctggcag aaatccaaga acctggtggc gccatcaacc ctggagcgtc tctggtcgcg
     1801 gcattttgcc gattcggcgc agctcagcgg acttgcgcca aaggcgcgac tctggctgga
     1861 tctcggctcg ggggcaggct ttcccgggct cgtcatcgct attctcgaag ccgggaggcc
     1921 ggatttccgc atgcacctgg tcgaaagcaa ccgcaagaag tgcgccttcc tgggcgaggt
     1981 cgtgcgcgcg accaaggccc ctgtggacgt tcacgccatg cgcattgaag acctcgccga
     2041 aaacgcgcac agcctccggc cggacgtggt gagcgcgcgg gcgcttgcac ctctgccgca
     2101 tctcctcgaa ctcgccgccc cgttcttcgg gccggagacc agggggctgt tcctgaaagg
     2161 gcgcgagata gagaccgaga ttgcggaggc gcgaaggcgc ttcgattaca gctgccgcct
     2221 ccatccgagc ctcaccgcga cggactccca tattgtcgag gtgacagcgc tccgccggcg
     2281 cggcgaggga gccggcgcgc gatgaacgcc ttcggatccc gctgcatgcg tgttcttgct
     2341 ctcgccaatc agaagggcgg ggtgggcaag acgacgacgg cgatcaatct cgggacggcg
     2401 ctcgcggccg tcggcgagcg cgtgctgatc atcgatctcg atccgcaagg caatgccagc
     2461 accgggctcg gcgtggacga gcgtccactc tcgaccttcg atgtcttgag cgggcaggcg
     2521 gcgctttcgg cggcgagggt cccgacccac gtgccgcggc tttcgctcgt gccggcgacg
     2581 atcgacttga tgtcgttcga gcgcgagtcg ggggccggaa gcgggaggca ttaccgtttg
     2641 cgcgattgcc tcgccgcgat gagcgcggac gaaaccccgg agcttgcgac gacgtacgtg
     2701 ctcatcgact gtccgccctc gctcaattta ctcaccgtta acgctctcgc cgccgccgac
     2761 gccgtgctcg tgccgttgca atgcgagttc ttcgcgctcg aggggttggc ccagctcctg
     2821 agcacggtcg aggagatcag ggtgcgcctc aacccgaagc tccagatcca cggcatcgtc
     2881 ctcaccatgt acgaccagcg caccagcctc tccgaccagg tggttgacga cgtgcggcgc
     2941 gtcttgggcg acaaggtcta tgccaccatc atcccgcgca atgtgcgcgt ggcggagtcg
     3001 ccctcgcacg gaaagcccgt gttgctctac gattatcgct gctccggcag ccaggcttac
     3061 atccagctcg cctccgaggt gatcgagcgc gagcggcgga tgcgtgcggc gtgaggcatt
     3121 agcggcatgt caaacgaagg aggctcgcca tggcggtagg cgcgcagaag acgagactgg
     3181 ggagggggct ggcgtcgctg atcggcgatc gcttcgacga agcggcgctc ggcgccgagg
     3241 aggatcggcg aaccgttccg ctcgtcgcgc tcaagcccgg ccgcttcaat ccgcggcgca
     3301 attttgccga ggagcagctg gaggagctcg ccgcctcgat ccgcgagcgc ggactggtgc
     3361 agcccatcgt ggtcaggccc tcgtcgggcg acacgtatga gatcgtcgcc ggcgagcgcc
     3421 gctggcgcgc ggcccagctc gccgatcttc acgaggtgcc ggtcatcgtc cgtacactga
     3481 atgatcagga ggcggtggag atcgccatca tcgagaacgt gcagcgcgag gacctgaacg
     3541 cgatcgagga aggggagggg tacaagctgc ttatcgaggg ccacgactac acgcaggaag
     3601 acctcgcgcg cattgtcggc aagagccgca gtcacctcgc caatacgctg cgcctgttga
     3661 agcttcccga gagcgtacag gatctcgtgc gctcgggcgc gctgagcgcg gggcacgtgc
     3721 ggcctctcgt cgggcgcacc gacgccgcag cacttgcaga gcgcatcgtg agccgcggat
     3781 tgaacgtgcg tcaggtcgaa gctctcaccc aggatcgcgg tcccgccaag accaggcggc
     3841 agggaagcaa ggatgccgac acgcgcgccg ccgaagcgga gctgcatgag gctctcggcc
     3901 tcgaggtcga gatccgcaaa ggcaaaggcg agaagggaga gcttcgcatt cgttatgcga
     3961 cgctcgacca actcgaggat ttgcgtcacc gcctcctccg ccggtagtga tcttggtgga
     4021 gcggcgatct cgatgcgctg acgcggcaaa cacgtcattc cggcggaagc cggaatccag
     4081 aagcggcatg cactgcattt gaagttctgg ataccggctt tcgccggtat gacgaatgaa
     4141 ccccccactc ggctcagcga gcgaccgcga gaaactcctc ggcgcggaac agcgaagcga
     4201 acgggatgcc ggccttggca taaagatcgg cggcgccctc gccgcgatcg aggatcgaaa
     4261 gcacgagcgc cacttttgcg ccggcctctt ccacggcctt gaccgcatcc atcgccgatc
     4321 cgccggtggt ggtcacgtcc tccaggatga tgacgtgctt gccggcgatg tcggcgccgt
     4381 cgatgcgctt cttcgtgccg tgatccttga cggccttgcg cacgaaaaag ccgtggaggc
     4441 ggcggtcgaa atcgaggctc cgcattgcga caggggcgat gagcgggacc gcgcccatct
     4501 ccagcccgcc cacgcagtct gccttaacat cttgaatttc ctgaagaatg agctctgcca
     4561 tcagcgcggc gccgtcggga tcgagcatcg ccggcttcat gtcgaaataa aaatcgctct
     4621 cgcgccccga ggcgagcacc acgcggccgc gccggaacgc gcgctccttg agcagcgcaa
     4681 aaagcttggt ccgcaactcg acggtgttgg cgcgctcctt catgccatac ctcgctcttc
     4741 gcgacatgct tacaacgccg cgcgccttcg tcaaaccttc gaggtgagcg agagcacgag
     4801 acgctcggcg aaggcgcctg caagatcggg cgaaaggcgc gagcgcttga tcgcttcctg
     4861 aatcaggggc agcgctcttg ccaggcgcgc agctccccat ttgcggcagc ggacaaggaa
     4921 ggcctcctcg cgcgtccgtg gacgcggcgg aggcttcagc gaacggagcg cctcctcggg
     4981 cggtcgtccg ctcgcgaccg ctgcagccac gcgatggagc tgaatgaaat ggcggccgag
     5041 cgcagacagc gcggcaccct tgtccgtgcc cgacgcgaca agccgttgaa gctccgacag
     5101 cgccgccttc gcgtcgccgc cgctcgtggc ataaacgaaa ttttcgagtg cggtctcggc
     5161 ggcgtcgccg acgatggctt cgacgtcgtc gtggttgacg gcccccgccc cttgcgcata
     5221 gagcgcgagc ttgacgactt ccgcgcgcga caaggcctgg tcggcgccga gccgcgtcat
     5281 cagatagtcg cgcgtttcgc ggtcgatgct gagaccggca tcgccgagct cggcttcgat
     5341 cagcgctgac aggtcgcgcg cgtcgctgta gcacggaagc gccgcggcgc ttttgtgagc
     5401 ctcgaaggct ttgcgcaagg cagagtcggg acgaagattg cccgcttcca cgatcaggac
     5461 gtttgcgaaa ctctccgcaa gcaaaatctt gagcgcgggt acatcgagct tcggcccggc
     5521 cgcgacgcgc acgacgctct gctcggcgaa catcggtacg gtccgtagct cgacctcgat
     5581 ccgcgccggg tcctcggcga gatcgcggtc gtcgaggcga acgatctcgg tcgctccccg
     5641 cccccgacgc gcgaagactt gcgcaagcgc gtcggcgcgt tcggccacga gcccggcgtc
     5701 gggcccgtac acgagcacgg cgcggcaatc ggccttcggc gccttgacga agctcgcgac
     5761 ggctggcgat ttgtagacga ccatgcgcgt gaagcggctg cggttagacg agggtgacga
     5821 cagcgaaagg actaagcggc gttgctgaga tgcgccgcga cctgggtcct gatgctctcc
     5881 gacaccgtac gggcggcgcg gttctcggcg tcgtagcggg cgcgcacgtt agcgaagatt
     5941 tcctggaagc gattataggg cgcccgcgag attgccttgc cttggtacac gaccacgccc
     6001 ttcgaagcgt cgatgagttt gaacgtcgcg tcgagttggt acacttgccc ggtcgcgtcg
     6061 ccggtaatct gcaccagctg atcggtgacg ctttccttga cggcgatgtc gagccggtag
     6121 cgggtcgggc ccgcagtgcc cccgccggta ttggcgaaga tcagctcgtt gcgcagcctc
     6181 tggccgacgc ggcccgggat gggcgcgacg tcgatcccgg ccatgacctc cgaaatcctg
     6241 gcgccaccgg ccgtgctccc gccgtagagg ggctggaagc cgcagccggc cagcgcgagc
     6301 gctccaagcc cggcgagcag cgaggccaaa acggcaaggc tacgccacca cattgacgat
     6361 cctctgcggc acgaccacca ccttcttgat tttgcgtccc ccgacggcgc gaaccacatt
     6421 ttcgagcttt agcgccgctg cttcaatatc ttcctttgcg gcgtcccgcg caatcgtcaa
     6481 ttcgtcccgc cttttgccat tgacctggac cgcaattgtg acggtttcct caagcaaaag
     6541 ggccttttcc ggggccggcc agggctcctc ggcgaggagc gtctcatggc caagctcagc
     6601 ccagcattcc tcggccaggt gggggatcat gggggaggcg attcgggcaa ggagctccgc
     6661 ggcctcccgc aaggcatagg cctggtcggg ggcagggtgt ggcccggcgc tttgcaagcc
     6721 tgaggagagg gcattggcga gctcgtggat ctgggcgacg gcggtattga aacgaagctt
     6781 ctcgatggcg gccgtcaccg ccgcgaggct gcggtgagcg gcgcggcgaa gcgcctcggc
     6841 ccccgggccg aactttgcgg gcttcgcgcc cgaaggcggg gcgcccttgg ccgcggcctc
     6901 cctgaccagc cgccacatcc gctggaggaa gcggtgcgcg ccctcgacgc ccgcttccga
     6961 ccattcgatg tcgcgttcgg gcggcgtgtc ggacagcatg aaccagcgcg cggtgtcggc
     7021 cccataggcg gaaatgatgg tttcgggatc gaccacgttc ttcttcgact tcgacatcga
     7081 ctcgatggcg ccaacggtca ccggctcgcc cgtctttgca tgcaccgcct ctccgtcttt
     7141 gcggctcacc tcctcgggca gcagccattt tccgtgtacg tccttatagg tctcgtgcgt
     7201 caccatgccc tgcgtgaaca ggccgcggaa cggttcgtcc atggccacgt gtccggtctt
     7261 gtgcatggcg cgagtaaaga agcgcgcgta gagcagatgc aggatcgcgt gctccacgcc
     7321 gccgatatac tgatcgacgg gaagccagtg tcgagtcgcc gcaagatcgg ttggctcgtg
     7381 cgatttcggc gcacagaagc gcacgaaata ccaagacgaa tcgacgaacg tatccatcgt
     7441 gtcggtctcg cgcatagcct tgccgccgca ttgcggacag ctgacgtgct tccatttcgg
     7501 atggtggtcg agaggattgc cgggtctgtc gaagctcaca tcttcgggaa gcttcacggg
     7561 gagatcgctc tcgggcaccg gcacgatccc gcatgacgga caatggatca tcgggatcgg
     7621 gcagccccaa tagcgctgcc gcgaaatgcc ccagtcgcgc aagcgatact gaacctggcg
     7681 gtcgccgaca ggcttgccct tgagtttttt cttggtgaga cgttcggcga cggcgtcttt
     7741 cgctgccgcg acgtcgagac cgtcgaggaa gctcgaattg atcaggaggc cgtcgccggt
     7801 ataggcctct tcgcagatcg cgaagcgctt tggattttcg ccaggcggca gcacgacggg
     7861 aaggacggga agcccctcgg cgcgcgcgaa ttcgaggtcg cgctgatcgt gcgccgggca
     7921 gccgaagatg gcgcccgtgc cgtactccat gacgatgaaa ttggcgatga agacgggaag
     7981 cgtagcccct tcgatcaacg ggtgacgcac gcgcaggccc gtatcgaaac ccttcttctc
     8041 ttgcgtctcg atctctgccg cgctcgtgcc gtgacgatgg cattcgtcga tgaaggcgcc
     8101 ggcctccgca ttcgtttccg caatcgcttt ggcgagcgga tgatcgggcg caatcgcaag
     8161 gaagcttgcg ccgaacagcg tgtcggggcg cgtcgtgaac acctcaagca cgtcatgacc
     8221 ttcgggcggc gcgccttcga cggcgaagcg gacgagaagt ccctccgagc ggccgatcca
     8281 gttcgcctgc atcagccgga ccttgtccgg ccagcgctcg aggcggtcga gcgctcgaag
     8341 cagatcctcg gcgtagcggg tgatcttgaa cacccattcc ggctgctcgc gctgctcgac
     8401 gagcgcgccc gagcgccagc cgcgcccgtc gatcacctgc tcgttggcga gcacggtctg
     8461 gtcgatcgga tcccaattga ctttgcgcgt cttgcggtcg acgagacccg ccttgaggag
     8521 atcgaggaac aggcgctgct gctggtggta gtagtcgagg tcgcaggtgg cgaactcgcg
     8581 cgtccaatcg atcgacagcc cgaggcgctt gagctggccg cgcatgacgg cgatgttgtc
     8641 gtaggtcgat ttggcgggat gaatgccgcg ctcgaaagcc gcgttctccg ccggcagacc
     8701 gaacgcatcc cagcccatgg gatgcagtac gttgtggccc ttggcgtgca tatagcgggc
     8761 gacgacgtcg cccatggtgt agttccgcac atggcccatg tggatgcggc cggatgggta
     8821 ggggaacatt tcaagaacgt aatatttcgg ccgtcggtcg tcctccgacc cgcggaagag
     8881 accgcgctcg tcccagacag cctgccaatg cctctctcgt ctcagtgcgt cgtagcgttc
     8941 cgccgccata ggtctcgctc gtctcgtttg tgcatgcgga atgaaccaga atggcgagag
     9001 cggtcaagtt acgcgcgtcc tcaagccttg ctagatagtg gatatgggat cggaccctct
     9061 tgaccccgtc cacgtctcgg agcgcctcgc gctcgtgcgt caggaaatcg cgcttgccgc
     9121 cgaggccgcg ggccgcgcgc cccacgcggt caatctggtc gcggtgacga agacggtggc
     9181 ggcgcccgtc atcgccgaag cggtcgcggc gggccagcgt tgcttcggcg aaaaccgcgt
     9241 gcaggaagct catgccaaat ggccggcgtt gaaacagagc catgccgata tcgagctgca
     9301 tctggtcggg ccgctgcaaa cgaacaaggt gcgcgaggcc gtcgccctgt tcgatgtcat
     9361 cgagaccgtg gaccggccga agctcgcgcg cgcgctggcc gaagaaatga tgcgctccgg
     9421 caaacgcccg cgcctcttcg ttcaggtcaa taccggcgag gagccgcaga aggcgggcgt
     9481 ggtcccgagc gaagccgaga ctttcatcgc gctctgccgc gacaccttcg ggctcaccat
     9541 cgctgggctg atgtgcattc cgcccctcga cgaggagccg gcgatgcatt tcgccctgct
     9601 cgccaagatc gccgagaagg ccggtgtgac ggaattgagc atgggcatga gcggcgattt
     9661 cgcccgcgcg atttccttcg gcgccaccta tgtgcggatc ggcaccgcga tcttcggccc
     9721 gcgcgacgaa tgagtcagac gctcaccacg atcgccgagc gcgggcgcac atgcgcaagc
     9781 agcatcgtga ggtcgtggcg cgaaagcgcg atgcagccgt cggtcggcgt gaagccggga
     9841 cgggcgaggt gaacgaagat ggcgctgcct ttccccttga cgcgcggcac gtcgttatag
     9901 ccgagcacga gcacgagatc gtagaggcgg tcggcgcgct tcaagccttc ggcgctccgc
     9961 cgcgacggca gcctgacgag acggttgtag ttgcgatcgg ccggatcgtc gcaccagccg
    10021 tcatcgtcgc ggatggcacg cacgggaagg cgcgtccgcg gtcgtgccac gcggtcggcg
    10081 cggtagagga cgtggcgaac ggcaaaccgc ccgagcgggg ttccgccatc gccttcgcgc
    10141 ttaattgacc gaatgcccgt gcggccgagc gccgccggtc tcacgccatg tccgagcacg
    10201 atccgcgcct gccagcggaa tctcggcgcg ggcctgacga agaccgatcg gggtcgctta
    10261 accctcatga ttcagacctt ctacgcgccg gtggggcttc gcgacaggct tgtccagcgg
    10321 ctttgccccc cttacaactc gggacgaaac aggctcgagg caccatgagc aacgcccgca
    10381 agatcctcat catcgacgat gacgacgagc tgcgcgagtc cttgagcgag cagctaagcc
    10441 tgcatgagga gttcgatacc gtcctggcgg cgacggcgtc cgagggcgtg aagcaggcgc
    10501 gtagcggcca tatcgacctc gttcttctcg atgtcggact ccccgacctc gacgggcgcg
    10561 aagcgtgcaa gctgctccgc cgcggtggct tcaagggccc tgtcgtcatg ctgacggcgc
    10621 aaacgtccga cgccgacacg atcctggggc tcgaatcggg ggccaacgac tacatcacca
    10681 agcctttccg cctcggcgtg ttgctggccc ggatccgcgc gcagctcagg cagcatgagc
    10741 agagcgagga cgcggtcttc gccatcggtc cttacacgtt caagcctgcc gccaagcttc
    10801 tggtcgacgg agcgggcgcg aagatcaggc tgacggaaaa ggagacctcg atcctgaaat
    10861 atctctatcg cgcgggcgag cgggtcgtga cccgcgacgt gctcctgcat gaagtgtggg
    10921 gctacaatgc cggcgtcacc acgcacacgc tcgaaacgca tatctaccgt ttgcggcaga
    10981 agatcgagaa ggacccgtca caggccgagc tgctgatcac cgagaccggc ggttacaagc
    11041 tcgtcccgta gccagccgcg gcgaacaatc actcaatcac gaagtcggcg agaaccggca
    11101 catgatccga tgtgcgcggc cagtcacgcg ttgcgtcgag gaccgtcatc gagcgcgccg
    11161 tgccgtcgag tgaaggcgtc gtccaaacat ggtccaggcg ccggccgcga ttggaggcgc
    11221 gccagtcgcg attgcggtag ctccaccagg tgaacagctt ctcctcggcg ggaacgtgcc
    11281 ggcgcatcac gtcaacccag ccttgcgacg cctggacacg ctcgaggcac tcgacctcag
    11341 ccggcgtgtg gctcaccact ttgagcagct gacggtgcga ccacacatcg gtcggaagcg
    11401 gcgcgacgtt gagatcgccg accatgatca tgcggctctg gcgctttccg cgcatgcctt
    11461 cgaaccagca ggcgagcgcc tcgaggaatt cgagcttgtg gcggaacctg tcgttgagcg
    11521 ccgggtcggg gatgtcgccg ccggcaggca catagacatt gtgcagcaga atcggctgcg
    11581 cgcccgcgcc gtcgatcgcg atggagagat ggcgcgcgtg accgcgcccg caaatatcgc
    11641 gccgctcttt cagcgtgaac gggatgcggg agagcgtggc gacgccgtga tacccctttt
    11701 ggcccgcgac cgcgatatgg ggaaagccga gcgcgcgaag ctcgcgcgcg ggaaagtgcc
    11761 cctcgatgca tttgatctct tgcaggcaga ggaaatcggg ccggtgggct ctcacgaagc
    11821 ggcgcacgag atcgagtcgg agtctgacgg agttgatgtt ccaggtggcg agacggaagc
    11881 gcatgggccg aaggatcggt ttgcacgcgg gcaaaagcaa gcgagcccct tgccgcgtcc
    11941 acacgtcgcg cgaaggtgcg gcagctcata aagaagaagc cccggagacg gggggactcg
    12001 gggcttctcc ggttccgtat ggggggaacc tgtgacgggg gagggaaacc ccgccactgc
    12061 aacctaaata gccggtgacg gggccatttc aaccccctcc aagtaagttg caagtattcg
    12121 atggccttaa ggatgcgatg ccttcaaggt tgcgccgttc aattcgtcca gagactgacg
    12181 gatgacggcg acgcttccct gcaaagcgag cgccgccctg gcggccgcaa cgatcacgtc
    12241 gggccatagg gtgcctgtcc cgaacacgcc gagcgccgcc gccaagaccg cgagattgcc
    12301 gagcacgtcg ttgcgcgagc agatccaggc cgaacgaagg ttcgcttccg actcgcggaa
    12361 ccggtagagc aagccgagaa cgaggccgtt tgcagcgagc gcaagcgctc cgatcccgcc
    12421 catgagaaga ggctcgggca cgcggccgac gacgatgtgc cacacggtct cgccaataac
    12481 ccaaaggccg agcaaggcca tggttgcccc cttcaacagg gcggctcttg cgcgccagct
    12541 cagcgcaagg ccgagcacgg ccaggctgat ggcgtagttg aacgtgtcgc cgagaaagtc
    12601 gagcgcgtcg gcgtgaaggc tgacggaccc cgcgatgacg ccggcgacga tttcggtgag
    12661 gaacatcccc gcgttgatgg cgagggccgc ccacaagacg gggcgagccg atatcggcgg
    12721 cttatgatcg cagcactgcg cacccacggc taacgctatc tcaaacgcct tgggtcaacc
    12781 aacaggccaa aagacgagaa ggcaaagaag ccggctaagc ggtcttctta gtgtctggca
    12841 cgccccactg gtcaacgagg gctttggcct gttccaagtc cgttgctagg cgcgtccagg
    12901 ttttcgcgag ttcctcgaac cttgccttgg tcagtgcaga cggggaggtc gccgcgatct
    12961 ccaagcatcg cttggcgtgc ccacggcact gtttcggatc accgggcatg tgactctcct
    13021 acgactttca cacgccaaga ttcgattccg caaacaaggt cctacgtcaa ggggctgatc
    13081 gtgcggtgcg aaagaagaag ccccggccaa ggtgggtcga ccggggcttc cccaaccaaa
    13141 ggagtgatcg cagggacgat cacaccgcaa ccctgatagc acaaccccgg ccaagtgagc
    13201 cggggttttt ggcttagcca tcgcgccctc catcgtttca accgagggtt aggatggcat
    13261 gggttgggaa gtacaatatc gccaaagaaa aggcgcccaa ccgctgcgcg tggttgggcg
    13321 ccagaagcgc gttttcgcgc gcgtgccggg agggaagccg gcacgagcga accacatctt
    13381 cggctagacg gggggcacta ccgatgaaaa gcgatgtggt ccgcttctag aaccaacaaa
    13441 tatgtagatc ggcgggcgat ttcaacgccc gtcggccgaa aaatttaacg agacgtaaag
    13501 aacg
//
LOCUS       DASUQP010000040         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_426554,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000040 DASUQP010000000
VERSION     DASUQP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426554"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            872..1159
                     /locus_tag="VFY74_01910"
     CDS             872..1159
                     /locus_tag="VFY74_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930494.1"
                     /translation="MPRLVLKLVVFACMHHGAAIIGGNALAAGTELSSCLDAATILGA
                     GGDVSDQELTAAQGACTRLKQSSTDRDTLARVKAAAETIDEEVQRRGGSAR"
     gene            complement(1192..1449)
                     /locus_tag="VFY74_01915"
     CDS             complement(1192..1449)
                     /locus_tag="VFY74_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930495.1"
                     /translation="MRYLTDRYRPELHYMRGPGPKWLEKHARQSGALVVQSEGHQASA
                     APLRRIALAFRRWIPARWRAAYTAFREMPWNPADRLGPWSV"
     gene            1701..>2103
                     /locus_tag="VFY74_01920"
     CDS             1701..>2103
                     /locus_tag="VFY74_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930496.1"
                     /translation="MVMNTRRRALGLLVGAAAALRFPRLPRAAAAEKDCFDSKTFGPW
                     KAQASDTQAGARINAVPFTNDACDLRIEIQVAASFEGKIVVYGDPEKTRLPKKVLINA
                     DNRLIAWSQDGKEAVNEPLCGNCTDIFDDKVS"
BASE COUNT          386 a          677 c          662 g          378 t
ORIGIN      
        1 ctcccgcatc gggcctatcc ttcctgcaac ttgcgaatcc aggggaggct aaattgccgc
       61 aaggtttccg cctcaaacgg tagacttctt gtcgctagaa atccagcgga ccgggacgtc
      121 gcggtgcggc cgccgcaaat cattgttggt aacggcggcg taaaggcgag caaagacctg
      181 ggcgaaggac ccttcactgc catgacgggc gctaggccgt caaagggcac gccctcgctg
      241 cggtacggct tcaccatgat ccccgagatc gattcggagg gtgcctggaa cagccaggtc
      301 atcgatgcga ttcccggtga cacgggtcgc caactgtcgg cagccgatcg tggaggggag
      361 ccttgtcgcg cctcccgttg aaacgcgccg ccacgagccg ctcgtctcgg tcgccttctg
      421 gccaccggag gcgccctcca cttttaacct acaagagtaa agagtcgctt gaacgccgaa
      481 ccaaggcggc tcctattcgg cttcaagagc tccgggggca tggtgacttg gcgagccgct
      541 tgctcgttct tttccgctaa tcgagcgagt atgcggacac agtttgcctt ccgtgcaaac
      601 gccgcttgct gctccagcgg gatgaggcag tcccctgccg ctttctcata agccgccgcg
      661 agttgaacgt gttcttctgc cgtgagcgcc atcgcccgaa tcatccatca tggcggttaa
      721 ggcgcggtta cgctgacgac gctcggggtt tggactatcc aaggtcatgc catcccaatg
      781 aaagtcgccg cagcaagcac tctacacact tggggcgtgg tcgtgggttc gctacagttc
      841 ggcctctgat gccgcaaccc ggagggacct aatgccgcgt ctcgttctta aactcgtcgt
      901 gttcgcttgc atgcatcatg gcgcggccat catcggcgga aacgcgcttg cggcgggtac
      961 cgagctatcc agctgcctcg atgccgcgac gatcctcggc gccggaggcg atgtcagcga
     1021 ccaggagctc acggcggctc aaggggcctg cacgcggctc aagcaatcct caaccgacag
     1081 ggataccctc gcgcgggtca aggcggcggc cgagacgatc gacgaggaag tccagcgccg
     1141 cgggggttcg gcccgctgac cgcaggttgg aaaatcgcga tgaagcgccc gctagaccga
     1201 ccacggcccg agccggtcgg ccgggttcca tggcatctcg cggaaagccg tgtaggcggc
     1261 gcgccatcgc gccggaatcc accggcggaa cgcgagagcg atgcggcgga gcggcgctgc
     1321 gctcgcctgg tggccctcgc tttggaccac gagcgctccc gactggcgcg catgtttctc
     1381 cagccatttg ggtccaggcc cgcgcatata gtgcagctca ggccggtagc gatccgtcag
     1441 atatcgcatc tctcaatccc cgaagccccc ctcgccggcc cgactcgagc cggcgcgttc
     1501 acccgagagt gggcgcaagg tcgttgccaa cgggtttatt cctgcgatgg acgcgcgttt
     1561 ctctcggccc aaggttaatc gacggtacct ttggcgctcg cgcgcccgcg cgcgcaacct
     1621 gtggccgcga acagcgatcg tttgcgtgaa ggacccgtta tcggctaggc aagacggctt
     1681 caaatcggga tgtaacagcg atggttatga acacgcgccg ccgggcgctc gggcttcttg
     1741 tcggtgctgc ggccgccctg cggtttccgc gtctaccccg tgccgccgcc gccgagaagg
     1801 actgcttcga ctcgaaaacg ttcggaccct ggaaggcgca ggcgagcgac acgcaagcag
     1861 gcgcccgcat caacgcggtg ccattcacca atgacgcgtg cgatctgcgc atcgagattc
     1921 aggtcgcggc gagtttcgag ggtaagatcg tcgtctacgg cgatccggaa aagacgcgcc
     1981 tgcccaagaa agtcctcatc aatgccgaca accgcttgat cgcctggagc caggacggca
     2041 aggaggcggt gaacgagccg ctttgcggca actgcaccga cattttcgac gacaaggtga
     2101 gca
//
LOCUS       DASUQP010000041        14675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_427359,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000041 DASUQP010000000
VERSION     DASUQP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 14675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14675
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427359"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1801)
                     /gene="aspS"
                     /locus_tag="VFY74_01925"
     CDS             complement(26..1801)
                     /gene="aspS"
                     /locus_tag="VFY74_01925"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5930497.1"
                     /translation="MHLYRTHNCGALRKGDVGKTVRLSGWVHRVRDHGGLLFIDLRDH
                     HGLTQAVADPQSPAFAVAEKLKPEWVVRVEGEVVARTPETVNPNLPTGAVEVRIASLD
                     VLSEAKELPLPVFGEPDYPEETRLKYRFLDLRRETLHANIMKRQAIIWSLRNRMREAG
                     FTEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLIMIAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDLEMSFVTQEDVFETVEPILRGLFEEFGGGKPVTKKFPR
                     IPYAEAMRKYGTDKPDLRNPIEMHDVTEHFRGSGFKVFAGMLDMDVNARVWAIPGKGG
                     GSRAVCDRLNAWAQEEDQPGLGYVFFRDGEGAGPIAKNIGPERTAAIKAELGLADGDA
                     VFFVAGLPGEFADFAARARVKVGTELKLTTTDRFEFCWVVDFPMYEWNQEEGRIDFSH
                     NPFSMPQGGLEALETKDPLDILAYQYDVVCNGVELSSGAIRNHKPEIMEKAFAIAGYP
                     REELEKRFGGMLRALQYGAPPHGGIAPGIDRMVMLLCGEENLRQVVMFPMNQQAEDLM
                     MGAPSEVSPKQLKELHIRVVPPPKA"
     gene            1984..3180
                     /gene="rnd"
                     /locus_tag="VFY74_01930"
     CDS             1984..3180
                     /gene="rnd"
                     /locus_tag="VFY74_01930"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX5930498.1"
                     /translation="MNLISKTDELADACARLGGASYIAIDTEFIREQTFWPRLCLIQI
                     ASDGTELLIDSLAPGLDLAPFFDLMVSERVLKVFHSARQDIEIVHHLAGVIPHPIFDT
                     QVAAMVCGFGEAVSYAMLVKRLLNRNLDKTSRFTDWSRRPLSERQLTYALGDVVHLRD
                     LYPKLRAQLDKSERASWLNEEMAVLTDPATYELHPEHAWRRLKMRTKTPKSLAVLMEL
                     AAWREREAQTQDVPRARILKDEALYDIASQAPRTMEDLGSLRTLHNGFARSMRGRAVL
                     EAVGRGLERDPKTIPPIERGEPMPPEAQAVVDLLRVLLKARAGSHGVAPKLIATSEEL
                     EEIARSEGADAPVLRGWRKKLFGDDALALKRGELALVIKKGEVAVIAPKGSPDPLAAE
                     KPKDQG"
     gene            complement(3148..3345)
                     /locus_tag="VFY74_01935"
     CDS             complement(3148..3345)
                     /locus_tag="VFY74_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX5930499.1"
                     /translation="MKSIGATLLFLLAVAAITFLSVRYGLGRLPGDIIIDRGGVVVYL
                     PITTAVLISVTLALVLWLFRR"
     gene            complement(3345..3677)
                     /locus_tag="VFY74_01940"
     CDS             complement(3345..3677)
                     /locus_tag="VFY74_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930500.1"
                     /translation="MMLTERDLLTRVQRLTVTRLRVWVAQGWIKPADQAAQSYSEADL
                     ARAALICNLEDELGFDEDDVPVLLNLIDQIHGLRAELKSLLDALEDLPPDLRATVRMR
                     ITRGREGG"
     gene            complement(3674..4606)
                     /locus_tag="VFY74_01945"
     CDS             complement(3674..4606)
                     /locus_tag="VFY74_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5930501.1"
                     /translation="MAEDLYSVLGVAKTAKAEDITKAYRKLAKKLHPDLNPGDKSAEE
                     RFKQITAAYDILGDEEKRGRYDRGEIDASGQETPQQRYYREYAGGAEGARYRSTAGYE
                     DIGAFSDLFGDLFGEGGGMRGRAGGRRFSMRGQDAQIRLEVGFLDAVNGTKTRITLPD
                     GGTLDVTIPPGVTDGQVLRLKGKGGPGLGEGEPGDALIEIAVRPHAVFKREGDDIVVD
                     VPITLDEAVLGGKVEVPTIGGRVSVTVPPGANSGQTLRLKGRGVKAKGAAGDQLVKLS
                     VVLPERIDEDLKSFAERWRQGHSYDPRRKLKEQA"
     gene            complement(4652..6169)
                     /gene="ppx"
                     /locus_tag="VFY74_01950"
     CDS             complement(4652..6169)
                     /gene="ppx"
                     /locus_tag="VFY74_01950"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX5930502.1"
                     /translation="MVPTPELAPIAVVDIGSNSVRLIVYENTGRAPTPIFNEKILCGL
                     GRALASTGKLAPEAVERALRALRRFRALSDQLGVGRIEVIATAAAREAANGPEFIAQA
                     ERILGRDITVLSGTREAELAGLGVVSGIHAADGFAGDLGGGSLELIDVRGGRLGDAAT
                     LPLGGLRLIDAAGGSLKKAREIVDAELAQVEWLEKGRGRDFYSIGGTWRALARLHMTQ
                     TNYPLSVMHNYRINADDALRFAALLDHQSQSSLAGIRDISSARRETLPYGALVLERLI
                     KQMKPRSVVVSVFGIREGLLYSLLSEDEKRKDPLIAACGDIARRRSRSLDSAYELCFW
                     TDALFRAPGPSETPEQRRLRHAACLLSDVGWRAHPDYRGIQSLNLVAHSTFVGVDHPG
                     RAFLALSVYYRNEGLVKDEASQRMIELVDKDMLKRARILGAAFRAAHMVSAAMPGVLT
                     NTPIAYEGNRLVWTLPDPYSNLEGERVERRFKVLAGLLDREAEIRAGSSWQRAAS"
     gene            complement(6186..8366)
                     /locus_tag="VFY74_01955"
     CDS             complement(6186..8366)
                     /locus_tag="VFY74_01955"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX5930503.1"
                     /translation="MSLSEKVERLDVSPGLAAFGPRRFFNRELSWLQFNRRVLEEAEN
                     ERHPLLERLRFLSISASNLDEFYMVRVAGLRGQVLAGIETPSDDGLTPVEQLDRINRS
                     VAELTAEQQENWVTLEALLAEAGIHVLSADQLSEAERSWLEEHFLAHIFPVLTPIAVD
                     PAHPFPFIPNFGFTLGLELVREGSLKTMHALMPIPPQLDRFIRLPSKGETREPIRFIR
                     LETVVGIFVSRLFPGYRVRSQGAFRVLRDSDIEVQEEAEDLVRLFESALKRRRRGSVI
                     RLEMEATMPSRLQRFVMQELKVSERESFVQKGLIGLADTNQLIVADRPDLKFQPFVSR
                     FPERIREHHGDCFAAIRKKDIVVHHPYESFDVVLQFLKQAAADPDVVAIKWTLYRTGK
                     TSPIIEALKEAAEAGKSVTAVVELKARFDEEANIKWARDLESAGVQVVYGFIELKTHA
                     KLSLIVRRERGELVTYCHVGTGNYHAVTAKIYTDLSFFTADRVIGHDLSRIFNYVTGY
                     AEPAELEAMAVSPHGLRARILDHIREEMYHAKAGRPAAIWMKMNSLVDAAIIDALYEA
                     SRAGVEIDLVVRGICCLRPGVPGLSETINVKSIVGRFLEHSRIYCFGAGHGLPHRKAV
                     VYISSADMMPRNLDRRMEAMVPITNPTVHEQILDQIMVTNLKDNLQSWRVQPDGSSER
                     IRPGKDQEPFSAQDYFMTNPSLSGRGTSLTEHMPPRFTRQAETG"
     gene            complement(8491..9168)
                     /locus_tag="VFY74_01960"
     CDS             complement(8491..9168)
                     /locus_tag="VFY74_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX5930504.1"
                     /translation="MTLDQLTLDLPHRAALGAEDFLVSDCNRAAVKLIDAWPQWSDQV
                     QLLIGPPASGKTHLARVWQALSRAKALHADTLRMDFVDGMRTGTPLVVEDADRGAYEE
                     KALFHLLNLAREKGLFVLLTARSAPSRWELSLPDLLSRLNAVPAVEIGAPDEALIRTV
                     MLKHFADRQLDIDPKVLTYLSLHVDRSLAAAAAAVEAVDRAALATGRKISRQLVSEVL
                     AGRGPSD"
     gene            complement(9165..9782)
                     /locus_tag="VFY74_01965"
     CDS             complement(9165..9782)
                     /locus_tag="VFY74_01965"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5930505.1"
                     /translation="MNIPNSLTLGRIVLVPLIVWLIITHEMMAAFLVFLLAGLSDAAD
                     GFLAKRYGWRSELGAYLDPIADKALLVSIYVTLGFAGHLPVWLVIAVVSRDILIVGAV
                     VLSWMLTRPLTMQPLLVSKANTLAQIVLAGLVLAELGLGLGLHPLVKVLIWVTGALTI
                     TSAAVYFWQWLGHMARYEPAPLPLPPRQRKTAKLDEPQPSRVRAS"
     gene            complement(9888..11036)
                     /locus_tag="VFY74_01970"
     CDS             complement(9888..11036)
                     /locus_tag="VFY74_01970"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEX5930506.1"
                     /translation="MAAPPLRRYALAIGLLLPCFASAAFLGGIARAADSLYDVAKISV
                     DITAEDAVAARDMGMAEAQARAVKTVLQRLLPVGVDADLPGLTKEDIEGLVNGVSIRK
                     EQNSTTRYIANLDVSVNEQAIKQLLQDYSLPYSEERAQPTSILPLMIAGGAVAGEGAE
                     GWRQAWDDLDLSHSITPATILRPRPGLGLDTVNAVLAGDAEALAKMQDEYGSPLVIAV
                     GEIAGGAFITRLAGADGVGQIAFERSDPVGGDPNVTAREAAAIALGIIENRWKTTGTG
                     GAPGAEANNQPGAGGAPDAGREGPKGEIPRNIVAQVEFSGLKEWQEIRGRLTNVAGIQ
                     GLEVNSLSARAASITFDYAGPLGRLQQELDQNGFVFDDSEGTFVLRSR"
     gene            11217..12263
                     /gene="purM"
                     /locus_tag="VFY74_01975"
     CDS             11217..12263
                     /gene="purM"
                     /locus_tag="VFY74_01975"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5930507.1"
                     /translation="MSYRDAGVDIDTGNALVEAIAPLAAATKRAGVLGALGGFGGLFD
                     LKASGFADPILVAATDGVGTKVKLAIETGIHDGIGLDLVAMNVNDIVVQGAEPLFFLD
                     YIACGKLELGVATAVIGSIAKGCKEAGCALIGGETAEMPGLYVPGDYDLAGFAVGAVE
                     RDGLLPREDLEAGDVIIALPSSGVHANGFSLVRRIVADAGLRWNDPAPFAREVTLGQT
                     LLTPTRIYVRQVLETIRRTRAVKALAHITGGGLTDNIPRVVPKHLSAEIDLAAFPLPP
                     VFAWLMQEARLPQDEMLRTFNCGFGMILIVAPREVEHVLQALDGEAMVIGAIVERGSG
                     APIRYTGTLARSAP"
     gene            12260..12931
                     /gene="purN"
                     /locus_tag="VFY74_01980"
     CDS             12260..12931
                     /gene="purN"
                     /locus_tag="VFY74_01980"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5930508.1"
                     /translation="MTKTRVGVLISGRGSNLRALIEACKAPTFPAEIVLVVSNVPRAP
                     GLAYAETVSIPTRTIDHKDFPSREAFDAALSEALDEARVELLCNAGFMRLHSESFVRH
                     WWNRHLNIHPSLLPAFPGLHTHARVLEAGAKISGCTVHFVRPQMDTGPIVAQAAVPVL
                     PGDTEDSLAARVLAAEHRLYPHALRLVASGAIRVEGEIVAGLMRSKEEAPHSPLFSPS
                     LDEGL"
     gene            13167..14531
                     /locus_tag="VFY74_01985"
     CDS             13167..14531
                     /locus_tag="VFY74_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5930509.1"
                     /translation="MADMTVAYSPSHGLGEEQSIWFKLTPLLNQENPMQVLMALSDKY
                     GGVIPINLKNQRVMLLSEPAHAERVLVSNADNYVKYFDGLRPIFGKSMITVDGALWQR
                     IRAPQQPAFHPHMFEAYFPYLMSAIDSKMARWAKFASTGETIEMVEETWTLAAEMVCK
                     ALFDREMPFNPHFVFGQVKTYTDVMNHKSIRLKNVRGELEEMTQEDAAKAMEVWGQIP
                     EMVTGAGVLDHREKTLLAMLEAAEADPSFPEFDRQQVVDEMKQYLWAGTETTALTLAW
                     SLYLLSRHPEALERIRAEARAVCGERDPQWNEIQQLSYTRMVIQETMRLFPPIWALIR
                     IAADDDEIEGHKIKAGDKIVILAYVMHHSPRYWDEPEKFDPDRFAPERAKKRVKYSYL
                     PFAAGKRACIGGALSQIENTLALVQLLRRFTPEYAGPVPAKIHATVTLCPKGGLPFRI
                     RALS"
BASE COUNT         2582 a         4837 c         4688 g         2568 t
ORIGIN      
        1 gcgctttgtg ataatgcgcc gttaatcacg ccttgggcgg tgggacgacc cgaatatgca
       61 gttccttcaa ctgcttaggt gacacctcgg acggcgcccc catcatcagg tcctcggctt
      121 gctgattcat cgggaacatc accacctggc gcagattttc ctcgccgcag agcagcatca
      181 ccatgcggtc gatgccgggc gcgatgccgc catgcggcgg ggcgccatat tgcagggcgc
      241 gcaacatgcc gccgaagcgc ttctccagct cctcccttgg atagcctgca atggcgaacg
      301 ccttctccat gatctcgggc ttgtggttcc ggatcgcgcc cgaggagagt tcgacgccgt
      361 tgcagacgac atcatactga taggcgagaa tgtcgagcgg gtccttcgtt tcgagcgcct
      421 cgagcccccc ctgcggcatc gagaagggat tgtgcgagaa gtcgatccgc ccttcctcct
      481 ggttccactc atacatcgga aagtcgacga cccagcaaaa ctcgaaccgg tccgttgtgg
      541 tgagcttgag ctctgtgccg accttcaccc gcgcgcgggc agcgaaatcg gcaaactcgc
      601 ccggcaatcc cgccacgaag aaaaccgcgt cgccgtcggc gaggccaagc tcggccttga
      661 tggcggcggt ccgttccggc ccgatattct tggcgatggg tcccgcccct tctccgtcgc
      721 ggaagaaaac atagccgagg cccggctggt cttcctcctg cgcccacgca ttgagccggt
      781 cgcagacggc gcgcgacccg ccgccttttc ccgggatcgc ccacacgcgc gcattgacgt
      841 ccatgtcgag catgccggcg aacaccttga aacccgagcc gcgaaaatgc tcggttacgt
      901 cgtgcatctc gatcgggttg cgcagatccg gcttgtccgt gccgtatttg cgcatcgcct
      961 cggcgtaagg gatgcgcggg aatttcttgg tgacgggctt gccgccgccg aactcctcga
     1021 acaggccgcg caggatcggc tccaccgtct cgaacacgtc ttcctgagtg acgaagctca
     1081 tttccaggtc gagctggtag aactcgccgg gcgaccggtc ggcccgggcg tcctcgtcgc
     1141 ggaaacaggg tgcgatctgg aagtagcggt cgaagcccgc gatcatgatc agctgcttga
     1201 attgctgcgg cgcctgcggc agggcgtaga acttgccggg atggacccgg ctcggcacga
     1261 ggtagtcgcg cgcgccttcg ggcgacgacg cggtgaggat cggcgtctga aattcggtga
     1321 agcccgcctc gcgcatgcga ttgcgcagcg accagatgat cgcctgccgc ttcatgatgt
     1381 tcgcgtgaag cgtttcccgg cgaagatcga ggaagcggta tttgagccgc gtctcctcgg
     1441 gatagtccgg ctcgccgaat accggcagcg gcagctcctt cgcttccgac agcacatcga
     1501 gactcgcgat cctgacttcg accgcgccgg tcggcagatt gggattgacc gtctcaggcg
     1561 tgcgcgcaac cacctcgccc tcgacccgca ccacccattc gggcttcagt ttctctgcga
     1621 cagcgaaggc cggcgattgc ggatcggcga cggcctgggt caggccatga tgatcgcgga
     1681 ggtcgatgaa aagcagcccg ccatggtcgc gcacccggtg cacccagccc gagagacgga
     1741 cggtcttgcc gacgtcacct ttgcgaagcg cgccgcaatt gtgagtgcga tagagatgca
     1801 tgagcgggat cggagcgggt tgcgcccgga aggaatagaa agcgccgcgg aacagcgcat
     1861 gggaggcctt ggttgtcaag gcgagcgggg cctgcggaaa gagcacaggg cggcggattg
     1921 acccgatttt ttgcgccgtc gcacccttgg cctcgcgcgt gggcttgggc tatacccgcc
     1981 tcgatgaacc tgatctccaa aaccgacgaa ctcgccgacg cctgtgcccg tttggggggt
     2041 gcctcctata tcgccatcga caccgagttc atacgcgagc agaccttctg gccgcggctc
     2101 tgcctgatcc agatcgcctc ggacggaacc gagctcctca tcgacagcct cgcgcccggt
     2161 ctcgacctcg cgcccttctt cgacttgatg gtctccgaac gcgtgctcaa ggtctttcac
     2221 tcggcgcgcc aagacatcga aatcgtccat catttggccg gcgtcatccc ccacccgatc
     2281 ttcgacaccc aggtcgcggc catggtgtgc ggcttcggcg aggcggtgag ctacgcgatg
     2341 ctggtgaagc ggctcttgaa ccgcaatttg gacaagacct cgcgcttcac cgattggagc
     2401 cggcgcccgc tctccgagcg ccagctcact tacgcgctcg gcgacgtcgt ccatctccgc
     2461 gatctctacc ccaagctccg ggcgcagctc gacaaatccg agcgggcgag ctggctaaac
     2521 gaagagatgg ccgtcttgac cgaccccgcc acctacgagc ttcatcccga gcatgcctgg
     2581 aggcggctca agatgcgcac caagacgccg aagtcccttg ccgtgctcat ggagcttgcc
     2641 gcctggcgcg aacgcgaggc gcaaacacaa gacgttcccc gggcgcgcat cctgaaggac
     2701 gaggcgctct acgacattgc gagccaagcg ccccgcacga tggaagatct cgggtcgttg
     2761 cgcacgctgc ataacggctt tgcccgctcc atgcgcggcc gcgccgtgct cgaagcggtc
     2821 gggcgcgggc tcgagcgcga tcccaagacc atcccgccga tcgagcgcgg cgagccgatg
     2881 ccgccggaag cccaggccgt ggtcgatctg ttgcgggtgc tgctgaaagc gcgggccggc
     2941 agccatggcg tggctcccaa gctcattgcc acaagcgaag aactcgaaga gatcgcgcgc
     3001 tcggagggcg ccgacgcgcc ggtcttgcgc ggctggcgca agaaactgtt cggcgacgat
     3061 gcgctggcgc tgaagcgcgg cgagcttgcg ctcgtcatca agaaaggcga ggtcgccgtg
     3121 attgcgccaa agggcagccc cgatccgcta gcggcggaaa agccaaagga ccaaggctag
     3181 cgtcacgctg atcaagacgg ccgtggtgat cggcaggtag accaccacgc cgccccggtc
     3241 gatgatgata tcgccgggaa gacgcccgag cccatagcgc acgctgagaa aagtgatcgc
     3301 cgcgaccgcc agaagaaaga ggagtgtggc accgatcgac ttcatcaacc accctctcgg
     3361 ccgcgcgtga tccgcatcct gaccgtggcg cggagatcgg gcggcaagtc ttcgagcgcg
     3421 tcgagcaagc ttttgagctc ggccctgagg ccatggatct ggtcgatcag gttgagcagg
     3481 accggcacgt cgtcttcatc aaagccaagc tcgtcttcga gattgcagat gagggccgcg
     3541 cgcgcgagat cggcctctga atagctctgc gccgcttgat cggccggctt gatccagcct
     3601 tgcgccaccc acactctgag ccgggtcacg gtcaggcgct gcacgcgggt gaggagatcg
     3661 cgctcggtca gcatcatgcc tgctccttga gcttgcggcg tggatcgtag ctgtgacctt
     3721 gccgccacct ttccgcgaag ctcttgaggt cctcgtcgat ccgctcgggc agcaccacgc
     3781 tcagcttgac gagctgatcg ccggccgccc ccttcgcttt cacgccgcgg cctttcagtc
     3841 tcagcgtctg gccgctattc gcccccgggg gcacggtgac agacacgcgg ccgccgatgg
     3901 tcggcacctc gaccttgccg ccgagcaccg cctcgtcgag agtgatcggc acgtcgacga
     3961 cgatgtcgtc gccctcgcgc ttgaacaccg catgcggtct caccgcgatc tcgatcagcg
     4021 catcgcccgg ctcgccttcg ccgagtcccg ggcccccttt gcctttcagc ctgagcactt
     4081 ggccatcggt gacgccgggc ggaatggtga cgtcgagcgt gccgccgtcg gggagcgtga
     4141 tccgcgtctt ggtgccgttc accgcgtcga ggaatccgac ctccagccga atttgcgcgt
     4201 cctgtccgcg catggagaag cgccgcccgc cggcacgccc gcgcatcccg cccccctcgc
     4261 cgaacagatc gccgaagaga tcgctgaagg cgccgatatc ctcgtagccc gctgtcgagc
     4321 ggtagcgggc gccttccgcg ccgccggcat attcgcggta atagcgctgc tgcggcgtct
     4381 cctgtccgga tgcgtcgatc tcgccgcggt cgtagcggcc gcgcttctcc tcgtcgccga
     4441 ggatgtcgta ggccgcggtg atctgcttga accgctcctc ggccgacttg tcgccgggat
     4501 tgaggtcggg atgaagcttc ttggcgagct tgcgataggc cttggtgata tcctcggcct
     4561 tcgcggtctt ggcgacgccg agtacggagt agagatcttc tgccatcgat aaatccaggg
     4621 ctggattgaa acgcaggagt caagtccagg ttcagcttgc cgcgcgctgc catgacgagc
     4681 cggccctgat ctccgcctcg cgatcgagca gtccggccag caccttgaac cggcgctcga
     4741 cccgctcgcc ttcgagattg gaatagggat cgggcaaggt ccacaccagc ctgtttccct
     4801 cgtaggcgat cggcgtattg gtcagcacgc cgggcatcgc ggccgagacc atgtgcgcgg
     4861 cgcggaaggc cgcgcccagg atgcgcgccc gtttcagcat atccttgtcg acgagctcga
     4921 tcattctttg gctcgcctcg tctttgacca gaccctcatt gcggtaatag acgctgaggg
     4981 cgaggaaggc gcggccgggg tgatcgaccc cgacaaaagt gctgtgcgcc accagattga
     5041 gactttggat gccgcgatag tccggatgcg cccgccagcc gacatcggag aggaggcagg
     5101 ccgcgtgccg cagccgcctc tgctccggcg tctcggatgg ccccggcgcc cggaacaacg
     5161 catcggtcca gaaacatagc tcataggcgc tgtcgagaga gcgcgaccgc ctgcgcgcaa
     5221 tatcgccgca ggccgcgatc agaggatctt tgcgcttctc gtcttcgctt agcagactgt
     5281 acagcaggcc ttcgcggata ccgaacaccg acaccacgac ggagcgcggc ttcatctgct
     5341 tgatgagccg ctccagcacg agcgcgccgt agggaagggt ctcgcgccgc gcgctggaga
     5401 tatcgcggat gccggcgagc gagctctgcg actggtgatc gagcagcgcg gcgaatctga
     5461 gcgcgtcgtc ggcgttgatc cggtagttgt gcatgacgct caagggatag ttggtctgcg
     5521 tcatgtgaag ccgcgccagc gcccgccacg tcccgccgat ggaatagaag tcgcggcctc
     5581 ggcccttctc gagccattcg acctgggcaa gctcggcgtc gacgatttcc cgcgccttct
     5641 tcaggctgcc gcccgcggcg tcgatcagcc gcagcccgcc gagcggcagg gtggcggcat
     5701 ctccgagcct gccgcccctc acgtcgatca actcaaggct gccgccgccc aagtcgccgg
     5761 caaagccgtc tgcggcatgg attccggaca cgacgccgag gcccgcgagc tcggcttccc
     5821 gggtccccga cagcaccgtg atatcccgcc cgaggatgcg ctcggcctgg gcaatgaatt
     5881 ccggtccatt ggcggcctcg cgcgccgcgg ccgtcgcgat cacctcgatc cggcccacgc
     5941 caagctggtc gctgagagcg cggaaacggc gcaaggcccg gagcgcgcgc tccacggcct
     6001 cgggcgcgag cttgccggtc gacgcgagcg cgcggccaag gccgcaaaga atcttttcgt
     6061 tgaagatcgg cgtcggcgcc cgccccgtat tctcatagac gatgagacgg accgagttcg
     6121 agccgatatc gaccaccgcg atgggggcca gctcaggggt tggaaccatg gcggcggagg
     6181 ggcgcttatc ccgtctcggc ttggcgcgtg aagcgcggcg gcatgtgctc ggtgagagac
     6241 gtgccgcggc cggacaggct cggattggtc atgaaatagt cttgagcact gaagggctcc
     6301 tggtcctttc ccggccggat ccgttccgaa cttccgtcgg gctgcacccg ccagctttgc
     6361 agattgtcct tgagattcgt caccatgatt tggtcgagaa tctgctcgtg cacggttggg
     6421 ttggtgatcg gcaccatggc ctccatgcga cggtcgagat tgcgcggcat catgtcggcc
     6481 gagctgatat agaccaccgc cttccgatgc ggaaggccat gaccggcccc gaagcaatag
     6541 atgcgggagt gctccaggaa gcgtccgacg atgctcttga cgttgatcgt ctccgacagg
     6601 ccggggacgc cgggtctcaa gcagcagatg ccgcgcacca cgaggtcgat ctcgaccccc
     6661 gcccggctcg cttcgtagag cgcatcgatg atcgcggcgt cgacgagcga gttcattttc
     6721 atccagatgg cggccggccg gcccgccttg gcgtgataca tctcttcgcg gatatggtcg
     6781 agaatgcgcg ccctgaggcc gtgcggcgaa accgccatgg cctccagctc ggccggctcg
     6841 gcgtagcccg tcacgtaatt gaagatgcgc gagagatcat ggccgatgac gcggtcggcg
     6901 gtgaagaaag acaggtcggt gtaaatcttg gcggtgaccg cgtgataatt gcccgtgccc
     6961 acgtggcaat aggtgaccag ctcgccgcgc tcgcgccgca cgatcaggct cagcttggca
     7021 tgggtcttga gctcgatgaa gccgtacacc acctggacgc ccgcgctttc caagtcgcgt
     7081 gcccatttga tgttggcctc ctcgtcgaag cgggccttga gctcgacgac cgcggtcacg
     7141 gacttgcccg cctccgcagc ctccttgagc gcctcgatga tcggcgaagt cttgccggtg
     7201 cggtagagcg tccatttgat cgccaccaca tcggggtcgg ccgcggcctg cttcaggaat
     7261 tgcagcacga cgtcgaacga ctcgtaaggg tgatggacga cgatgtcctt cttgcggatg
     7321 gcggcgaagc aatcgccgtg atgctcgcgg atgcgctcgg gaaagcggct gacgaagggc
     7381 tgaaacttga ggtcgggccg gtcggcgacg atcagctgat tggtatcggc aagcccgatc
     7441 agacccttct gcacgaagct ctcgcgctcg gaaaccttca gctcctgcat gacgaagcgc
     7501 tgaagccgcg acggcatcgt cgcttccatt tcgagcctga tgaccgagcc ccggcgacgg
     7561 cgcttgagcg ccgattcgaa cagccgaacc agatcttcgg cctcttcttg cacttcgatg
     7621 tcgctgtcgc ggagtacgcg gaaggcaccc tggcttctca cccgatagcc cgggaagagc
     7681 cgcgagacga agatgccgac caccgtctcg agcctgatga agcggatcgg ttcccttgtt
     7741 tcgcccttgc tcggcaagcg gatgaagcgg tcgagttggg gcgggatcgg catgagcgcg
     7801 tgcatggtct taagcgaccc ctcgcgcacg agctcaagcc cgagcgtgaa gccgaaattc
     7861 gggatgaagg gaaacggatg ggcgggatcg accgcgatcg gggtcagcac cggaaaaata
     7921 tgggcgagga aatgctcctc gagccacgag cgctccgcct ccgacagctg atccgcgctc
     7981 agcacatgaa tgccggcctc cgccagcagc gcttcgagcg tgacccagtt ttcctgctgc
     8041 tccgccgtca gctccgcgac ggagcggttg atgcggtcga gctgctcgac gggggtcagt
     8101 ccgtcgtcgc ttggcgtctc gatgccggcg agcacctgcc cgcgcaaacc ggcgacccgc
     8161 accatgtaga actcgtcgag attcgaggcc gagatcgaca gaaagcgcag gcgctcgagc
     8221 aaggggtggc gctcgttctc ggcttcttcg agcacgcgcc ggttgaactg aagccaggac
     8281 agctcgcggt tgaagaagcg cctcggcccg aaggcggcga gccccgggct cacatccagc
     8341 cgttcaacct tctccgacaa actcatcagt aacctcttgc aactaaacct ttttatcgcg
     8401 actgggcgcc atcaggtgcc gtgcgcggcc ccaatctagg gccaagaact gtatcacttg
     8461 cgttacagcg ggcgccaatc gcctcgcgtc tcagtcggac ggcccccgtc cggcaagcac
     8521 ttcgctcacc agctggcggc tgatcttgcg cccggtggcg agcgccgcgc gatcgaccgc
     8581 ttcgacggca gcggcggcag ccgcaagcga gcggtcaaca tgcagcgaga gatatgtaag
     8641 cactttcggg tcgatatcga gctgcctgtc ggcaaaatgc ttgagcatga cggtgcggat
     8701 cagcgcctcg tcgggagccc cgatctcgac cgcgggcacg gcattgaggc gcgacagcag
     8761 gtccggcaac gaaagttccc agcggctcgg tgcgctgcgc gccgtcagca gcacgaagag
     8821 ccccttctca cgcgccagat tgaggagatg aaaaagcgcc ttctcctcat atgcgccgcg
     8881 atccgcatcc tccacgacga gcggcgtccc cgttctcatc ccgtcgacga agtccatgcg
     8941 aagcgtatcg gcatggagcg cctttgcccg cgacagggcc tgccagacgc gcgcgagatg
     9001 cgtcttgccg cttgcgggcg gcccgatcag aagctggact tggtccgacc attgcggcca
     9061 cgcgtcgatc agcttgaccg ctgcccgatt gcaatcgctg acgaggaaat cctctgcgcc
     9121 gagcgccgcg cgatgcggca gatcgagcgt gagctggtcg agcgtcacga cgccctgacc
     9181 cgggagggtt gcggctcgtc gagcttggcc gtctttcttt gccggggcgg cagaggcaga
     9241 ggcgcgggct cgtaacgcgc catatgcccg agccactgcc agaaatagac cgcggccgag
     9301 gtgatggtca gggctccggt cacccagatg agcactttca cgaggggatg gagcccaaga
     9361 ccgagaccga gctcggccag gacgaggccc gcgagcacga tctgggcgag ggtgttggct
     9421 ttcgagacca ggagcggctg catggttagc ggccgcgtca gcatccacga cagcaccacg
     9481 gccccgacga tgaggatgtc gcggctcacc accgcgatca cgagccacac cggcaaatga
     9541 ccggcgaatc cgagcgtgac atagatgctg accagcagcg ccttgtcggc gatcggatcg
     9601 agataggcgc caagctcgct gcgccagccg taacgcttgg cgaggaaccc atcggccgca
     9661 tcggacagcc cggccagcag aaacaccagg aaggccgcca tcatctcgtg cgtgatgatc
     9721 agccacacga tgagcggaac gaggacgatc ctcccgagcg tcaggctgtt gggaatattc
     9781 acgaacgcgc ctcccggacg ccatttccgc cttgcggcac ggcgcctccg ggccgggagt
     9841 ttagcagatt ctcacatccg gcgtgaccgg cacctcgggc ccaatcctta acgcgaacgg
     9901 agtacaaacg tcccctcgct gtcgtcgaaa acgaagccgt tctggtcgag ctcctgctgg
     9961 agccggccga gcgggccggc ataatcgaag gtgatggatg ctgcgcgcgc cgaaagcgaa
    10021 ttgacctcga gcccctggat tcccgccacg ttcgtcagcc gtccgcggat ctcttgccac
    10081 tctttgaggc ccgagaattc aacctgcgcg acgatgttgc gcgggatctc gccctttgga
    10141 ccctcgcggc ctgcgtccgg cgccccgccc gcgcccggct ggttattcgc ttccgccccg
    10201 ggcgcgccgc cggtcccagt cgttttccag cggttctcga tgatccccaa ggcgatggcg
    10261 gctgcctcgc gcgccgtaac gttgggatcg ccgccaaccg ggtcgctgcg ctcgaacgcg
    10321 atttggccaa cgccgtcggc gccggcgagc cgggtgatga acgcgccgcc cgcgatctcg
    10381 cccacggcga tgacgagggg ggagccgtac tcgtcttgca tcttggccag ggcctcggca
    10441 tcgccggcga ggaccgcgtt aacggtgtcg agcccaaggc cgggacgcgg cctcaagatc
    10501 gtggcggggg tgatgctgtg ggagagatcg agatcgtccc aggcttgccg ccagccctcg
    10561 gcgccttcac ccgccactgc cccgcccgca atcatgagcg gcaggatcga ggtcggctga
    10621 gcgcgctctt cgctgtaagg gaggctgtaa tcttgcagga gctgcttgat cgcctgctcg
    10681 ttaacgctga catcgaggtt cgcgatatag cgggtcgtcg agttctgctc cttgcggatc
    10741 gacacgccgt tgacgaggcc ctcgatgtcc tccttggtga gcccgggcag gtcggcgtcg
    10801 acgcccacgg gaagcaggcg ctgaagcacg gtcttgacgg cgcgcgcctg cgcctccgcc
    10861 atgcccatgt cccgcgcggc gacggcgtcc tcggcggtga tgtcgacgga aatcttggcg
    10921 acgtcataga ggctgtcggc ggcgcgcgcg atgccgccca agaaggcagc agaggcaaaa
    10981 cagggcaaca ggagcccgat cgcaagcgcg taacgtctca gtggcggtgc cgccatctgc
    11041 cgaaatccgc agcgcatcaa ggtcgctcca tcccccttgg taaagcttgt gaatgtggcg
    11101 gacccacggc ctgttcaagc cttgacgggc ctcgccgggc acgattgtta tgccaaatcg
    11161 cctgcttctg caccccggat ggttcggtgc ccgcaactcc caaggacaag gccaaaatga
    11221 gctatcgcga cgccggcgtc gatatcgaca ccggcaacgc gctcgtcgag gcgatcgcgc
    11281 cgctcgccgc ggccacgaag cgggcgggag tgctgggcgc gctcggcggc ttcggcggcc
    11341 tgttcgatct caaggcctcg ggctttgccg accccatcct cgtcgccgcc accgacggtg
    11401 tcggcaccaa ggtcaagctt gcgatcgaga ccgggatcca tgacgggatc ggtctcgacc
    11461 tcgtcgccat gaacgtgaac gacatcgtgg tgcagggcgc cgagcccctg ttctttctcg
    11521 actacatcgc ctgcggcaag ctcgagcttg gcgtggcgac ggctgtgatc ggcagtatcg
    11581 ccaagggttg caaggaggcg ggctgcgcgc tgatcggtgg ggagaccgcc gagatgccgg
    11641 gactctatgt gcctggcgat tacgatctcg ccggctttgc cgtcggcgcg gtcgagcgcg
    11701 acgggttgct gccgcgggaa gacctcgaag ccggcgacgt cattattgcc cttccctcct
    11761 caggcgttca cgccaacggc ttctcgctgg tgcgccgcat cgtggctgac gcggggctgc
    11821 ggtggaacga ccccgcgccc tttgcgcgtg aggtgacgct cggccagacc ttgctcaccc
    11881 cgacgcgcat ctatgtccgg caggtccttg aaacgattcg ccgaacgcgc gcggtcaagg
    11941 cgcttgccca tatcaccggg ggcgggctga ccgacaacat tccgcgcgtt gtgccgaaac
    12001 acctttccgc cgagatcgac cttgccgcgt ttccgctgcc accggtcttc gcctggctca
    12061 tgcaggaagc gcggctcccc caggacgaga tgctccggac gttcaattgc ggtttcggca
    12121 tgatcctgat cgtcgccccg agagaggtcg agcacgtgct ccaggcactc gacggcgagg
    12181 cgatggttat cggcgccatc gttgagcgcg gaagcggcgc accgatccgc tataccggca
    12241 cgcttgcgcg gagcgccccg tgacgaaaac gcgcgttggc gttctcattt ccggtcgcgg
    12301 cagcaatctg cgcgcgctga tcgaagcctg caaggcccca acctttcctg ccgaaatcgt
    12361 gctcgtggtg tctaacgttc ctcgcgcgcc gggccttgcg tacgcggaaa cagtctcaat
    12421 cccgacccgc acgatcgatc acaaggactt tccgtcgcgc gaagcgttcg acgcggctct
    12481 gagcgaagcg ctggacgaag cgcgggtcga actcctgtgc aatgcaggct tcatgcgtct
    12541 ccacagcgaa agcttcgtac gacactggtg gaaccgccac ctcaacatcc acccctctct
    12601 gcttcccgcc ttcccgggcc tgcacactca cgcccgcgta ctggaagcgg gcgccaagat
    12661 cagcggctgc accgtccact tcgtacggcc ccaaatggac accggtccga tcgtggctca
    12721 ggccgccgtt cccgtcctgc cgggcgacac ggaggacagc ctcgccgccc gcgtgcttgc
    12781 cgccgagcac cgcctctacc ctcatgcgct tcgccttgtt gcctctggcg cgatccgcgt
    12841 ggaaggcgag attgtcgcag ggttgatgcg gtccaaagaa gaagccccac attccccctt
    12901 attctcgccg tccctcgacg aagggctttg attgaaattg ttaacggccg taagatcggg
    12961 gactggacgg ggcggcatgt cgcaccgcag aatatgcgcc gtgccgcttg tggacattgc
    13021 cgacggccgg atgctagaga ctggcccaaa tcgaggcagt ttgtgtctgt ttcccaagcg
    13081 aaaggccgct tttgcgacgg ccgggcgcgg aaaacaggcg agcggccccc ggaaccaaca
    13141 gcagggagcc cgggggaaag gcataaatgg ctgatatgac ggtcgcctat tctccatcgc
    13201 atgggctcgg cgaagagcag agcatttggt tcaagctgac cccgctcctg aaccaggaga
    13261 accccatgca ggttctcatg gcgctgagcg acaagtatgg cggcgtaatt cccatcaatc
    13321 tcaagaacca gcgcgtcatg ctcttgtcgg agccggcgca tgccgagcgc gtgctggtgt
    13381 cgaacgccga caattacgtg aagtatttcg acgggcttcg ccccatcttc ggcaagagca
    13441 tgatcacggt cgatggcgcg ctgtggcaga ggatccgcgc gccgcagcag ccggccttcc
    13501 acccgcatat gttcgaggcc tatttcccct atctgatgtc ggccatcgac tccaagatgg
    13561 cgcgctgggc gaaattcgcc tccaccggcg agaccatcga gatggtggag gagacctgga
    13621 cgctcgccgc cgagatggtg tgcaaggcgc tgttcgaccg cgagatgccg ttcaacccgc
    13681 atttcgtgtt cggccaggtc aagacctaca ccgacgtgat gaaccacaag tcgatccggc
    13741 tcaagaacgt gcgcggcgag ctcgaggaga tgacccagga agacgccgcc aaggccatgg
    13801 aggtgtgggg ccagatcccg gagatggtga caggcgcggg cgtgctcgat catcgcgaga
    13861 agacgttgct cgcaatgttg gaagcggccg aagccgatcc gagcttcccg gaattcgacc
    13921 gtcagcaggt ggtcgacgaa atgaagcaat atctgtgggc cgggaccgag accaccgcgc
    13981 tcacgctcgc ctggtcgctc tatctcctct cgcggcaccc cgaggcgctc gagcgcatca
    14041 gggccgaagc gcgggccgta tgcggcgaac gcgatccgca atggaacgag attcagcagc
    14101 tgagctatac gcgcatggtg atccaggaga ccatgcggct gttcccgccg atctgggcac
    14161 tcatccgcat cgcggccgac gacgacgaga tcgagggcca caagatcaag gccggcgaca
    14221 agatcgtgat cctcgcttat gtcatgcacc acagcccgcg ctattgggac gagccggaga
    14281 agttcgatcc cgatcgcttc gcgccggagc gcgccaagaa gcgggtgaag tacagctatc
    14341 ttcccttcgc cgccggcaag cgcgcttgca tcggcggggc gctgtcgcag atcgagaaca
    14401 cgctcgctct ggtgcagctg ctgcgccgct tcacgcccga atatgcggga ccggtgccgg
    14461 cgaagatcca cgcgaccgtc acgctgtgcc ccaagggcgg cctccccttc cgcatccgcg
    14521 ccctgagctg acggcctatc ccccttgaaa ggacggcgct gctctatttt gcgctaccgc
    14581 tgcgctacac cgaacagcgc cgggtattcg gacgcgctca ctggcaccca gttcatatcc
    14641 atagccctgg cctctaaatt ccccgagcaa gtgaa
//
LOCUS       DASUQP010000042         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_564463,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000042 DASUQP010000000
VERSION     DASUQP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564463"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="VFY74_01990"
     CDS             complement(<1..2007)
                     /locus_tag="VFY74_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5930510.1"
                     /translation="MNDQPQRTQFFGGCPHDCPDTCAMIYEVEDGRLVEVRGNKEHPM
                     TRGGLCVKLKDYHDHHYNPDRVLYPLRRVGPKGSRQFARIGWDEAIAEIAQRWKDIIA
                     RYGSQAIMPYSYLGNEGLVQGLTAGDAFFNRLGSTVNEKTFCASGSSTAWLLTVGPTG
                     GVDPESFVHSRYIVIWACNSISTNLHHWPFVLEAQKRGAKIVVIDSYRSRTAKQADWH
                     IMPKPGTDGALAMGIIAAMIRHGHVDREWVDKHTVGFPELAARAAEFTPDYVEKVTGV
                     PAQDIATFAREFATIQPSVIRMGVALERHAGGGQTIRAVCAIPALAGSWRHVGGGLLQ
                     MPLWEFPIDWARVSRPDFIRPGTRVVNNLQLGRALVGDMKLDPPIMGLYIYNTNPVSQ
                     APETNTIVKGLSREDLFFVTAEHFVTDTAAYADIVLPATMAGEHDDMMFSWGHFYLTI
                     NEQAIKPCGEAKSNAEIFRLLAASFGFDDPHFKMSDMELAEHYVKWDAPQMAGVDMAH
                     FRRHGYFHLAVGTPDDRLPHANGNFPTPSGKVEFLVKNAKNFVAPPFRMMYEAMQSGE
                     DVDPLPGYVPPRESAATNPRLAERYPLSVISPKSHGFLNSCYANEPHKIRGQGEQFVM
                     ISPKDAAARSIREGDPVRVFNDRGDFEGLARITDDVRDGVIVATL"
     gene            2493..3263
                     /locus_tag="VFY74_01995"
     CDS             2493..3263
                     /locus_tag="VFY74_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX5930511.1"
                     /translation="MGSISDVEGAAEIVSSGQSAGAADGAAVHMNDELKTGADGHLQV
                     TFRDDTVLTLGEDARVVIDRYVFDPDQGVGDVLLTTTQGAFRFATGRLKDLSRKDITV
                     ATPVAEIGIRGTEFWGGPVDGEYSVLLLAGEIDVKNQAGTVRLNRPGLATVIRSRLQA
                     PLRPSVWSVDRIERALTRTVTRKGLKQLERELRNFNLPGLRDGKDRLPDLLKQKRPDA
                     LKPDALKPDALKPKGRKPKIGPDGLQQRLRKPQLRLPW"
BASE COUNT          703 a         1182 c         1224 g          656 t
ORIGIN      
        1 gagcgttgcc acgatcacgc cgtcgcggac gtcgtcggta atgcgggcaa ggccttcgaa
       61 atcgccgcga tcgttgaaca cccgcaccgg atcgccctcg cggatgctgc gcgccgccgc
      121 gtccttcgga ctgatcatca cgaactgctc gccctgccct ctgatcttgt gcggctcgtt
      181 ggcgtagcag gaattgagaa agccgtggct cttgggcgag atgacgctga gcggatagcg
      241 ctcggcaagg cgcgggttgg tggcggcgct ctcccgcggc ggcacgtagc cgggaagcgg
      301 gtcgacatcc tcgcccgact gcatcgcctc gtacatcatg cggaaaggag gggcgacgaa
      361 gttcttcgcg ttcttgacca ggaactccac cttgcccgac ggggtgggga aattgccgtt
      421 agcgtgcggc agccggtcgt cgggcgtgcc cacggcgaga tggaaatagc cgtgccgccg
      481 gaaatgggcc atgtcgaccc cggccatctg cggcgcgtcc catttcacgt aatgctcggc
      541 gagctccatg tcgctcattt tgaaatgcgg atcgtcgaac ccgaacgaag cggcgagcag
      601 gcggaaaatc tcggcgttcg acttcgcctc gccacacggc ttgatcgcct gctcgttgat
      661 ggtgagatag aaatgccccc aggagaacat catgtcatcg tgctcgccgg ccatggtcgc
      721 ggggagcacg atgtcggcat aggccgcggt gtcggtgacg aaatgctcgg cggtcacgaa
      781 gaacagatcc tcgcggctca agcccttgac gatcgtattg gtctcgggcg cctggctcac
      841 cgggttggta ttgtagatgt agagacccat gatcggcgga tcgagtttca tgtcgccgac
      901 gagcgcgcgg ccgagctgga ggttgttcac cacgcgcgtg ccgggccgga tgaaatcggg
      961 acgcgacacc cgcgcccaat cgatcggaaa ttcccacagc ggcatttgca gcaagccgcc
     1021 gccgacatgg cgccatgagc ccgccagcgc cgggatggcg cagacggcgc ggatggtctg
     1081 gccgccgccg gcatggcgct cgagcgcgac acccatgcgg atgaccgaag gctgaatggt
     1141 tgcgaactcg cgcgcgaacg tggcgatgtc ctgcgccgga acgcccgtca ccttctccac
     1201 ataatccggg gtgaactcgg ccgcgcgcgc ggcgagctcg gggaagccga cggtgtgttt
     1261 gtccacccat tcgcggtcga catggccgtg tcggatcatc gcggcgatga tgcccatggc
     1321 aagagcgccg tcggtgccgg gcttcggcat gatatgccaa tcggcctgct tcgccgtgcg
     1381 cgagcggtag gaatcgatca ctacgatctt ggcgccgcgc ttctgcgctt cgagcacgaa
     1441 cggccagtga tgcaggttgg tcgagatcga gttgcaggcc cagatgacga tgtagcggga
     1501 atggacgaag ctttccggat caacgccgcc ggtcgggccg acggtcagga gccaagcggt
     1561 cgacgagccc gacgcgcaga aggtcttctc gttgacggtc gagccgaggc gattgaagaa
     1621 ggcgtcgccc gccgtgagcc cctgcaccag gccttcattg ccgagatagg agtaaggcat
     1681 gatcgcctgg ctgccgtagc gggcgatgat gtccttccag cgttgcgcaa tctccgcgat
     1741 ggcctcgtcc cagccgatgc gagcgaactg gcgcgagccc ttggggccga cgcggcgcaa
     1801 tggataaagc acgcggtcgg ggttgtaatg gtggtcgtga tagtccttga gcttgacgca
     1861 gagacccccg cgcgtcatcg ggtgctcctt gttgcccctc acctcgacca gccgcccgtc
     1921 ttccacctcg tagatcatgg cgcaggtgtc ggggcagtca tgcggacatc ccccgaagaa
     1981 ttgcgtccgc tgaggctggt cgttcatgct cagaaccctc cttggcgatc ccgcgaagcc
     2041 gctgaggccg tgagcctaag agacggacgc ccgcttgtcg acccgccatc gccacaagcg
     2101 caacgcatta cccccctgca ggagattatc cctcccctcg atggggaggg gggcgaagct
     2161 tgagcggagc gaaagctgag ccgggtgggg tgttggtgtt tcaacatcgc taccacttcg
     2221 caacttgagc gcaccgcccc caccccggcg cgcaagagcg cgccggacct ccccacgagg
     2281 gggaggtatg acgagaagca gaatctccgg cgaggacgcc aacttgacaa aattctgggc
     2341 ccatcgtcag gtttcgcgcc gttacctgag cagggcccgt gcacgccaca gcgcaactcc
     2401 gaatcttgcg atcgttcttt cggcgcgggt ccttccttcc ggcggttctt gtgcttatcg
     2461 cctcgctcgg cgcccacgcc gcggatgacg ccgtgggctc aatcagcgac gtcgaaggcg
     2521 cggccgaaat cgtgtcaagc ggccagagcg cgggcgcggc cgacggcgcc gccgtgcaca
     2581 tgaacgacga gctcaagacc ggggccgacg gtcatctgca agtgacattt cgcgacgaca
     2641 cggttctcac gctcggcgag gacgcgcgcg tcgtaatcga ccgctacgtg ttcgatcccg
     2701 accagggcgt cggcgacgtt ctgctgacga cgacacaagg agcgttccgc ttcgcgacag
     2761 gtcggctgaa agatttgagc cgcaaagaca ttacggtcgc gacccccgtc gcagagatcg
     2821 gtatccgcgg gactgaattc tggggcggtc ccgtcgacgg cgaatatagc gttctgctcc
     2881 ttgccggcga gatcgacgtg aagaatcagg ccggcaccgt gaggctgaat agaccgggac
     2941 ttgccacggt gatccgttcg cggctccagg cgccgctccg cccgagcgtc tggtcggtcg
     3001 acaggatcga acgcgcgctg acgcgcaccg tgacgcgcaa agggctgaaa cagctcgagc
     3061 gcgaactccg gaacttcaat ctccccggtc ttcgcgacgg caaggacagg ctcccggatc
     3121 tgctcaagca aaaacgcccg gacgcactca aaccggacgc actcaaaccg gacgcgctca
     3181 aaccgaaagg ccgaaaaccc aagatcggcc cggacggttt acaacagcgt ctgcgcaaac
     3241 ctcagttgcg gctcccttgg tgacgccgtt ttacgagtga tggagcgata ggtgagccgc
     3301 ttcggcgcgc actcgggcgg cttcgacttg ccccgcttgc gggtttggac catggggatt
     3361 gaggcgttcg cctgcgcggt ggtcatcgtg gcgtgcgcgc ggcaggcata cggcctgctc
     3421 actccccggc aggaacctcg cctcagggga tcacgccaga ggcatcgcca tcgcacgcag
     3481 cactttttta tctgatgcca atacacgcac tgaaaattgg ggcactgcca actaggaggc
     3541 ggtttgggca gacaaggaat tgtttactca gccgccaaag ccaccgggct actcgcgaac
     3601 gccgccacaa tggctagggc gcaaagcacg cgtgggagaa ctttgcgggc catgggaacc
     3661 tccatcgaca aactatcctt tcgatagtcg cttcagacga atggcaggtt cagctctgtc
     3721 gccatacggg caaaataaaa gggcccctgt ccagtggggg ccctt
//
LOCUS       DASUQP010000043         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1245723, whole genome shotgun sequence.
ACCESSION   DASUQP010000043 DASUQP010000000
VERSION     DASUQP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245723"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            890..2275
                     /locus_tag="VFY74_02000"
     CDS             890..2275
                     /locus_tag="VFY74_02000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5930512.1"
                     /translation="MLAAIAAAGLVAGGFYAYRVARVGSGFSAEILCGAVFVSGRTAE
                     EVKAKDLSGPGYELLRFFQQTVERDSKRVTASLYGFAAQTAIYREGLGCTLVDGKSEA
                     DLRAEAQALAVTPPADTGALWPEGERVEPDAQPKGVDRPALDAALAAAFAEPDPERPR
                     NTRALVVVHRGRVAAERYAPGFDAAMPLIAWSMTKGALNALVGLRVKDGKMALTDRAL
                     LPEWRGEGDKRRDISLDELLRMSSGLAFDESYDDPLADVTVMLFARGDMAKFAADKPL
                     LHPPGAHWDYASGTSLIIARALRATFATEEDYLRYPRERLFDPLSMRSAVLTSDAAGT
                     LVGSSLLYASARDFARLGLLFLQDGVWQGMRLLPEGWVEYTLAPAKDAPDETYGAHMW
                     LKLPLSDGYGEPPMPDDAYYFLGHDEQIIAVIPSRDLVIVRLGLTARGGAWDHARDLA
                     PIVNAFPPRHR"
     gene            2369..2875
                     /locus_tag="VFY74_02005"
     CDS             2369..2875
                     /locus_tag="VFY74_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX5930513.1"
                     /translation="MPFGMYQASVPAFVKMLNNLSAILDKAEGFAAERKIEPSVLLNW
                     RLAPDMFSLTRQIQIASDLAKGAAARLAGREVPSYADEEKSFADLKARIAKTVKFIES
                     VADKDIDGSEGRDIALSVGGQELRFKGAPYLVDFVLPNFYFHATTAYAILRRCGVDIG
                     KRDCLGAV"
BASE COUNT          530 a          970 c          935 g          548 t
ORIGIN      
        1 tggttgccaa gtcggagaat gagcaacagc cgcggctgca taaaacagag caacgaccat
       61 ggttatgaac gatagatttt tcatacgtct ctcctcaact atcggtcatg acgctattca
      121 caaaggcatt tatagggcca cggtacatac tgctcgcggc ctccatggcc ggtgtaacag
      181 tgaccccccg agaagttttt gagactatat ccgacccgtc ggccgtctcc gaagtcgccg
      241 gagcatacga agaatggttg ccgatgcagg cttggatcgc tgctcacgta ggtaccgctg
      301 ataacgggcc gggatgggcg gcacgcctca tcgcagcttt ggaagctcgc cccaagatct
      361 acccacgaaa gctccacctc tgaacaaagg cattgatagt tcggctcggg cctctcctga
      421 ccgccgtagc caacccagca gtgcttcgac caatttggtt tgagattata tccggctcgc
      481 aaaccctctc gggcggcgtt ggctcggcat gcgaagaact gttgcccatt caaccgcgct
      541 tcggtgctcc tgtaggtgcc gcttactacg gcgcgagaac gcggagcggg acagacctca
      601 tcacaaggcc tcctgttccc atctgcccag aaataggcaa acgcggcgtc cgaacaaaaa
      661 aagcgaggac tacagaagcg aacgctagag gtttcatgac tatctccgta gcgcgagatt
      721 cgtcattgcc tcccaatgcc gcgtgcggag tctagcataa gttgcggtgt ctagcatggt
      781 taaacgggga gttctgcctt aaagcagccg tgagttcaaa tctctccagc ggcaccacct
      841 cacccgcctc gcggcccgca tgatgacctt caagcgtagg acttggctca tgctcgcggc
      901 gatcgccgcc gcgggcctgg tggcgggagg cttttacgcc tatcgcgtcg cccgggtcgg
      961 ctccggcttc agcgccgaga tcctgtgcgg cgccgtgttc gtctcgggcc ggacggcgga
     1021 agaagtcaaa gccaaggacc tgagcggccc cggctacgag ctgttgcgct ttttccagca
     1081 gaccgtcgag cgcgacagca agcgcgtgac cgcctccctc tacggtttcg ccgcgcagac
     1141 cgcgatctat cgcgagggcc tcggctgcac gctggtcgac gggaagagcg aggccgactt
     1201 gcgcgcggaa gcgcaagccc tcgccgtgac gccacccgcc gacacgggcg cgctatggcc
     1261 cgagggcgag cgagtcgagc ctgacgcaca gcccaaaggc gtcgatcggc cggcgctcga
     1321 tgcggcgctg gcggccgcct tcgccgagcc cgatcccgag cgtccgcgca acacgcgcgc
     1381 gctcgtcgtc gtgcatcgcg gccgcgtcgc cgccgagcgt tatgcgccgg ggttcgatgc
     1441 ggcgatgccg ctcatcgcct ggtccatgac caagggcgcg ctcaacgcgc tcgtcggctt
     1501 gcgcgtcaag gacggcaaga tggcgctgac cgatcgggcg ctattgccgg agtggcgggg
     1561 cgagggcgac aagcgccgcg acatttcgct cgacgaactc ttgcgcatgt cgagcgggct
     1621 cgcgttcgat gagagctatg acgatccgct cgccgatgtg acggtgatgc tgttcgcgcg
     1681 cggcgacatg gcgaagttcg ccgccgataa gccgctgctt catccgccgg gcgcccattg
     1741 ggactatgcg agcggcactt cgcttatcat cgcgcgcgct ttgcgcgcga ccttcgcgac
     1801 cgaggaggac tatctgcgtt atccgcgcga gcgcctgttc gatccgctta gcatgagaag
     1861 cgcggtcctc acctccgatg ccgcgggcac gctcgtgggc tcctcgctgc tctatgcttc
     1921 ggcgcgcgac ttcgcgcgtc tcgggctcct cttcctgcaa gacggtgtgt ggcaggggat
     1981 gcggctcttg ccggaaggct gggtcgaata cacgctcgcg ccggcgaaag acgcgcccga
     2041 cgagacctat ggcgcgcata tgtggctcaa gcttccgctc tccgacggct acggcgagcc
     2101 gccgatgccg gacgacgcct attatttcct cggccacgac gagcagatca tcgccgtcat
     2161 cccctcgcgc gacctggtca tcgtgcggct cggcctcacc gcgcggggcg gcgcctggga
     2221 tcatgccagg gaccttgcgc cgatcgtgaa cgcgtttccg ccgcgccatc gctgagagac
     2281 ccgcgcggct cgccggaacg tgatcgaatg ccgtccgcgc ccgctgctac actccgcgcc
     2341 ttcaatgtca aaatgaggtg atgccgccat gccgtttgga atgtaccagg cctcggttcc
     2401 ggccttcgtc aagatgctga acaacctctc cgccattctc gacaaggcgg aagggttcgc
     2461 cgccgagcgc aagatcgagc cttccgtgtt gttgaactgg cggcttgcgc ccgacatgtt
     2521 ttcgttgacg cgacagattc agatcgcaag cgatctcgcc aaaggcgctg ccgcgcggct
     2581 cgcgggccgc gaggtcccga gttatgccga cgaggagaag agcttcgccg atctcaaggc
     2641 ccgcatcgcc aagacggtca agttcatcga gagcgtcgcg gacaaggaca tcgacggctc
     2701 cgaagggcgt gacatcgcgc tcagcgtcgg cgggcaggag ctgcgcttca agggcgcgcc
     2761 ctatctcgtg gatttcgtct tgcccaactt ctatttccac gcaaccaccg cctacgcgat
     2821 cctcaggcgc tgcggggtcg atatcggcaa gcgcgattgc ctcggcgcgg tttgagggcg
     2881 gcctttctat gccgtgttgc tggagctcca gaaggggcgc ccgagcaagg ccatcgccag
     2941 cccggcgacg aagccgccga tatgggccat ataggcgatg ccg
//
LOCUS       DASUQP010000044         4140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_700827,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000044 DASUQP010000000
VERSION     DASUQP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700827"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFY74_02010"
     CDS             complement(<1..844)
                     /locus_tag="VFY74_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5930514.1"
                     /translation="MADPVRVAVVGSGYFGRFHANHYARNARAELVTVVDTDPERARA
                     VAAEFGTEPAFDYRDILGKVDAASVAVPTPLHYDIASALIEAGIHVLVEKPLTDSVES
                     GARLACLAEARKAVLQVGHIERFSSAYRTLAKVIVDPLYFESYRIAPWKNRGVEVDVI
                     LDLMIHDIDMIIGLVDSPVAQVDAVGTPVLGKTIDLANARITFESGCIANVTASRVSY
                     KTERRMRIFARNHYLSCDLGEGRINGYRLRGDPMVEGLAAIAAETHEIEKQDSLANEI
                     DAFLD"
     gene            complement(860..2032)
                     /locus_tag="VFY74_02015"
     CDS             complement(860..2032)
                     /locus_tag="VFY74_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX5930515.1"
                     /translation="MSKDRTQAIPAFIDLAAQRERMGDRLEHAVSAVLKHGQYILGPE
                     VAVLEHELAAFSGARHCITCANGTDALLLALMAEGVGAGDAVFVPAFTFVATAEAAVL
                     CGATPVFVDVRPDTFNLDAESLEAAIGEAKRHGLTPRCVIPVDLYGQPADYRVIGAIA
                     ETHNLLMLADGAQSFGASLDGRKVGTLASYTATSFYPSKPLGGYGDGGALFTDDDERA
                     ARLRSLRNHGREPALPDTKHVGLNSRLDSIQAAILIEKLRLFPEEIPARQERARRYNE
                     ALAPVAKVPELMAGATSVWAQYTIVTERRDAVAKACRDAGVPTAIHYASALNCLAPYR
                     QFPTAPAGVPQAEWLAQRVISLPMHPYLGEDAQGLIVATVRQALGSGKAGVAHAAE"
     gene            2320..3489
                     /locus_tag="VFY74_02020"
     CDS             2320..3489
                     /locus_tag="VFY74_02020"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX5930516.1"
                     /translation="MLLGSERLIASQRLKGLRVGVLANPASIDRDFRHVVDALASSRD
                     WKLTAIFGPQHGFRSDLQDNMIETAHADDKRRKVPIFSLYSETREPTPAMLDLIDVLV
                     IDIQDVGARIYTFIYTMANCLRAAARKGLPVIVCDRPNPIGGVAVEGPMLEKDFESFV
                     GQFPIPMRHGMTIGELARLFNERHGIGAKLEVVPMEGWSRGMYWDETGLPWVMPSPNM
                     PTLDTAIVYPGTVLFEGTMLSEGRGTTRPFELIGAPWIDGEELASRMNAIGLEGAHFR
                     PAIFEPTFQKHARATCGGVQIHVTDRNAFEPVKAGVALMRECNEMAPDRFAWRPPPYE
                     YEHGKMPIDILAGSQALREQIAARMPLADIVASWRDDEAEFAKLRAPYLLYEAPR"
     gene            complement(3486..4094)
                     /locus_tag="VFY74_02025"
     CDS             complement(3486..4094)
                     /locus_tag="VFY74_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX5930517.1"
                     /translation="MPGLLKTGGAIIWSPLLGFVLALFLWLAVAWLFRRATPYAVDSK
                     FRVLQMVSAAAYSMGHGANDAQNDGHHRRLALRRGPAWRGIPRAVLGCHLLPGGDGAR
                     HAGWRVAHRQDDGSRITRLKPVQGFCPETAGAATLYLATSFGVPVSTTQTITGAIVGV
                     GAARRVSAVRWGIAGSLLIAWVVTLPAAAVIAASFYALATLI"
BASE COUNT          745 a         1363 c         1342 g          690 t
ORIGIN      
        1 agtcgaggaa cgcgtcgatc tcgttggcga ggctgtcctg tttctcgatc tcatgggtct
       61 ccgcggcgat cgccgcgagg ccctcgacca taggatcgcc gcgcaggcga tagccgttga
      121 tgcgcccctc gccgagatcg cagctcaaat agtgattgcg ggcaaagatg cgcatgcgcc
      181 gctcggtctt gtagctcacc cggctcgcgg tcacgttggc gatacagccc gattcgaaag
      241 tgatgcgggc attggcgagg tcgatcgtct tgccgagcac cggcgtgccg acggcatcca
      301 cctgcgccac cggcgaatcg accaggccga tgatcatgtc gatgtcatgg atcatcaggt
      361 cgaggatcac gtcgacctcg accccgcgat tcttccacgg cgcgatgcga tagctttcga
      421 aatagagcgg atcgacgatg accttggcga gagtccgata ggcgctcgag aaccgctcga
      481 tatggccgac ttgcagcaca gcctttcgcg cttcggcaag acaggcgagc cttgccccag
      541 attcgacgct gtcggtcagc ggcttctcga ccaggacgtg gatgccggct tcgatcagcg
      601 cgctcgcgat gtcgtaatga agcggcgtcg gcacggcaac gctcgccgca tcgaccttgc
      661 cgaggatatc gcgatagtcg aaggcgggct cggtgccgaa ctcggcggcg accgcacggg
      721 cacgctcggg atcggtatcg acgacggtga cgagctcggc ccgcgcgttg cgcgcatagt
      781 ggttggcgtg aaagcggccg aaataacccg agccgaccac ggcgacgcgg acaggatcag
      841 ccatggcgct caacgggtcc tactcggcgg catgggcgac gcccgctttt cccgagccga
      901 gcgcctgacg gacggtggcg acgatcaggc cttgggcgtc ttcgccgaga tagggatgca
      961 tgggaaggct gatcacgcgt tgcgcgagcc actcggcctg gggaacgcct gccggcgccg
     1021 tcggaaactg ccgatagggc gcaaggcaat tgagcgcgct cgcatagtgg atcgccgtgg
     1081 gaacgccggc atcgcggcag gctttggcaa cagcgtcgcg ccgctcggtc acgatcgtat
     1141 attgcgccca gaccgatgtc gctcccgcca tcagctcagg cactttagcg acaggcgcga
     1201 gcgcctcgtt gtagcgcctt gccctttcct gccgcgccgg aatctcttcc ggaaacaggc
     1261 ggagcttctc gatgaggatc gcggcctgga tcgagtcgag ccggctgttc agaccgacat
     1321 gcttggtgtc gggcagagcg ggctccctgc cgtgattgcg cagcgaacgg agccgcgcgg
     1381 cccgctcgtc gtcatcggta aacagagctc cgccgtcgcc ataaccgccg agtggcttcg
     1441 acggatagaa actggtcgcg gtgtaggatg cgagcgtgcc cacctttcgc ccatcgaggc
     1501 ttgcgccgaa gctctgcgcc ccatctgcga gcatgagaag attgtgagtc tcggcgatcg
     1561 cgccgatgac cctgtaatcg gccggctggc catagaggtc gaccgggatc acgcaacggg
     1621 gggttagccc gtggcgcttt gcctcgccga ttgcggcctc aaggctctcg gcgtcgaggt
     1681 tgaaggtatc gggacgcaca tcgacgaaga cgggggtggc gccgcaaagc accgccgcct
     1741 cggcggtcgc gacgaaggtg aacgcgggca cgaacacggc atcgcccgca cccacgcctt
     1801 cggccatcaa cgccagcaac agagcgtcgg tgccgttggc gcaagtgatg cagtgccggg
     1861 cgccgctgaa ggccgcaagc tcgtgctcaa gcacggcgac ttccgggcca agaatgtact
     1921 gcccgtgttt gagcacggcg gacaccgcgt gttcgaggcg gtcgcccatc cgctcgcgct
     1981 gcgccgcgag gtcgatgaag gcgggaatgg cttgcgtgcg gtccttgctc atgcaatctc
     2041 cacctgcccc ccttggagca ttctagcagc agaaatgacc atgtcggggg tccgaggtct
     2101 gggcgcggca ggcctcgaac ttggtcgatt ttcagcgatt tccctcaagt cttccggccg
     2161 gccccgccgc cgactaccat agcaggaccg aagtttcaaa cgccgcgacg ggccttcacc
     2221 ctttccgaaa cagtcatcaa gccgccagga gcgcttgaat gccagattgt catctcgtgc
     2281 gccgaacgtc ccgccgccag cgaaattccg cgtgagccta tgctgctcgg gtccgaacgc
     2341 ctgatcgcct cgcagcggct caagggattg cgggtcggcg tgctcgccaa tcccgcctcg
     2401 atcgatcgcg actttcgtca tgtcgtcgat gccctcgcct cttcgcgcga ctggaagctc
     2461 acggcgatct tcggccccca gcacggcttt cggtcggacc tccaggacaa catgatcgag
     2521 acggcccacg ccgacgataa gcggcgcaag gtcccgatct tctcgcttta tagcgagacg
     2581 cgcgaaccga cacccgcgat gctcgacctc atcgatgtgc tggtgatcga catccaagat
     2641 gtcggcgcgc gcatctacac cttcatctac accatggcca attgcctgcg cgccgcagcg
     2701 cgaaagggtc ttcccgtcat cgtctgcgac cggcccaatc cgatcggggg cgtggccgtc
     2761 gaaggcccca tgctggagaa ggacttcgaa tccttcgtcg gacagttccc cattccgatg
     2821 cggcacggca tgacaatcgg cgagctggcc cgcctgttca acgagcgtca cggcataggc
     2881 gccaagctcg aagtcgtgcc catggaggga tggtcgcgcg gcatgtattg ggacgagacc
     2941 gggcttccct gggtcatgcc ctcgcccaac atgccgacgc tcgacaccgc gatcgtctat
     3001 ccgggaaccg tgctgttcga aggcacgatg ctgtcggagg gccgcggcac cacccgtccc
     3061 ttcgagctga ttggcgcgcc ttggatcgac ggcgaagagc ttgcatcgcg catgaacgcg
     3121 atcggacttg agggcgcgca tttccgtccc gcgatcttcg agccgacctt ccagaagcac
     3181 gcgcgagcga cttgcggcgg tgtccagatc cacgtgacgg accggaatgc cttcgagccg
     3241 gtgaaggcgg gcgtcgcgct gatgcgcgag tgcaacgaga tggcgcccga ccggttcgca
     3301 tggcgccccc cgccttacga gtatgagcat ggcaagatgc ccatcgacat tctggccggt
     3361 tcgcaggctc tgagggagca gatcgcggcg cggatgccgc tcgccgacat cgtcgcgagc
     3421 tggagggacg acgaggcgga attcgcgaag ctccgcgcgc cgtatcttct gtatgaagcc
     3481 ccccgctaaa tcagcgtcgc caaggcatag aaggaagcgg cgatgacggc ggctgcgggc
     3541 agcgtgacca cccaggcgat caggaggctc cccgcaatgc cccagcggac cgccgagacg
     3601 cgccgcgccg ctccgacgcc gacgatggcg ccggtgatcg tctgcgtggt cgagaccggt
     3661 acgccgaagg acgtggcgag atagagcgtg gcggcaccgg ccgtctccgg gcagaagccc
     3721 tgcacgggtt tcagccgcgt gatgcgagag ccatcgtctt gacgatgcgc cacccgccaa
     3781 ccagcgtgcc gagccccatc gccgcctggc aggagatgac aacccagaac ggcacgtgga
     3841 attccccgcc aagcaggccc tcggcgtaga gcaagccggc gatgatgccc atcgttctgc
     3901 gcatcgttgg cgccgtgtcc catcgagtag gccgccgccg agaccatttg cagcacgcga
     3961 aacttgctgt ccacagcata gggcgtggcg cgccggaaga gccaggccac cgcgagccag
     4021 agaaagagcg cgaggacgaa acccagcagc ggcgaccata tgatcgcgcc tccggtcttg
     4081 agcagtcccg gcatacgaca gcgcccatcc ccgccttcgc cgtccccgct cccaccaggc
//
LOCUS       DASUQP010000045        14737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_428057,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000045 DASUQP010000000
VERSION     DASUQP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 14737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14737
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428057"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..459
                     /locus_tag="VFY74_02030"
     CDS             301..459
                     /locus_tag="VFY74_02030"
                     /inference="COORDINATES: protein motif:HMM:NF022716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEX5930518.1"
                     /translation="MTGAQASYLKLLAEEIQQPSAFAENLSKREASRRIDALAERLRL
                     CNLPPHTD"
     gene            596..1072
                     /locus_tag="VFY74_02035"
     CDS             596..1072
                     /locus_tag="VFY74_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX5930519.1"
                     /translation="MALTLTSSAFAPGEKIPAKYTCEGDDLSPPLAFTGVPDGTKSLA
                     LIVDDPDVPDPKAPKRIWVHWVVFNLPPDAQGLPEDVSGSGLPSGAVTGLNDRKKASY
                     QGPCPPIGRHRYFHKLYALDITLPAKQLSKSELEAAMKGHVLAQAELIGTYQKGDP"
     gene            1150..2367
                     /locus_tag="VFY74_02040"
     CDS             1150..2367
                     /locus_tag="VFY74_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5930520.1"
                     /translation="MTTVIHPIARAGIFAAVLIGLAPNAFAACASPAEFGRWLQGFKK
                     EAVAQGISPNTVSYALDGLTYDPATIAKDRAQGVFAQTFLQFSGRMVSSNRLQVGAQL
                     LKKHANTFNQIEQQYGVPGPVLVAFWGLETDFGKVMGNMETLRSLATLSFDCRRPDEF
                     REQLMFALKVVERGDLSPQEMRGPAHGEVGQFQFQPKNYYQYAVDFDGNGKRDLIRSA
                     PDSLASAANYLQSIGWQAGQPWLEQVRVPAELPWDQADVTIRKPRAFWAQHGVTYLNG
                     KPIPADDLQAGLVLPMGRNGPAFLAYPNFDVYLEWNKSLVYTTTAGYYATRLAGGPAL
                     ARGNGPVETLSLAETKQLQQLLAKRGFEVGKIDGVLGAASRDAIRSMQVKYGMPADAY
                     PTAELLSRLSAGQ"
     gene            complement(2417..3790)
                     /locus_tag="VFY74_02045"
     CDS             complement(2417..3790)
                     /locus_tag="VFY74_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEX5930521.1"
                     /translation="MKTYDAIVIGSGQGGVPLAANFAKLGWQVALIEEGQLGGSCINY
                     GCTPTKTMIASARIARAVRRGPEFGVHAGPVKIDLPAIVARKNEIVGSFRSGIENQVK
                     TNPNLTLYRGRGRFTGPHEVAVNGETLSSDRIFINTGTRPRIIPFPGLDEVAYLTNRN
                     IMDLTEVPPRLIALGGNYLGLEFGQLFRRLGSEVTVVELAKQIVPREDAEVAQSLKEA
                     LEEEGMNFRLGARAAKIAKTPGGLEVTIENKDGTIETLSGSHLLVAVGQTPNSDDLGL
                     DKAGVATDQAGFIRHNGRLETNVPGVWVLGDVKGGPAFTHVSYDDYLVITDNLVNGKN
                     RTIDNRVVPYALYTDPELGRVGLSEREARAKGYRLKIGSVPMAYVARAIERGETKGLM
                     KIVINADDDRILGATILGPEGGELVQILMALMMADAPWTLFKDAMFIHPTLTEGFFAL
                     MADVKAA"
     gene            complement(3811..4272)
                     /locus_tag="VFY74_02050"
     CDS             complement(3811..4272)
                     /locus_tag="VFY74_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5930522.1"
                     /translation="MPILINWLEAGRRLAERIPYSLVALVARLAVASVFWRSGRTKVE
                     GFLQIKDNTFFLFREEYKVPLLPDHAAYLATIAEHVFPVLLVVGLASRLSALALMAMT
                     LVIQFFVYPDGWPEHILWFALLLLIAARGPGALSLDHLIWSRAQPQLAAAR"
     gene            4530..5258
                     /locus_tag="VFY74_02055"
     CDS             4530..5258
                     /locus_tag="VFY74_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX5930523.1"
                     /translation="MTMAFATIEEAFRYCRDMDASLSERLETFANVARALRPEMQEAI
                     DRMVERLKAHNAGDSAPKPGEVMPPFTLPDETGRLVSLDSLLAAGPVAITFHRGHWCP
                     YCRINTRALSEAQENIKAEGAKIVAIMPDREQFAASFKLDADLHYPVLTDIDNGYALS
                     INLAIWVGAELERLMTAGGRDLPRYQGNDAWFLPIPATFVLASDGTVRTRFIDPDYRR
                     RMTVEDLLAALKNARNQERIGQGS"
     gene            5390..6109
                     /locus_tag="VFY74_02060"
     CDS             5390..6109
                     /locus_tag="VFY74_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX5930524.1"
                     /translation="MTSKPLSQLLDEAFVQCRDMDASLAERLQAFADAVRRFGPHFQD
                     AVDGLVNRLKQFEVGDNAPKPGEVMPNFVLPDENGQLVSLDSLLQKGPVAVTFHRGHW
                     CPYCRINTRVLAEAQHQIAAEGGQVAAIMPDREHFTTELKAESEAPFPILTDFDNGYA
                     LSLNLAFWVGQEMQELMTMAGWDVSRSQGTDTWLLPIPATFVVGTDGEVKARFLDPDY
                     RKRMTIEDLLSALRQARESAH"
     gene            complement(6106..6993)
                     /locus_tag="VFY74_02065"
     CDS             complement(6106..6993)
                     /locus_tag="VFY74_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5930525.1"
                     /translation="MEMHQVRYFLAVARVLNFTRAAEECHVAQPSLTRAIRQLEGELG
                     GDLFRRERPQAQLTELGQRMLPLLKQCYESAISARALASAIKKGEVGGLRLAVSRTID
                     PSLVTPHVVELRKLFEGLDLKILRGNGTEVLEHLKRGEVELGLAAQIGDEWERLDRWP
                     LFTEDFQLLVQSTHALAGRSTVSIDDLRQERLMVRTYCETTEALLEILRAQDFDVTRF
                     HEVGAEADLLSLLEAGVGTALVPRSTQGPATLVRIAVEGLELQRTIFLYGVAGRQRTA
                     VASALMKMLRAYDWTRSAA"
     gene            7402..8193
                     /locus_tag="VFY74_02070"
     CDS             7402..8193
                     /locus_tag="VFY74_02070"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX5930526.1"
                     /translation="MATSLRDKVSLVTGSTSGIGLGIARALAAAGSAVVLNGFGKPEE
                     IKVTQDAIEADFGVPVAYSDADMSKPDSIAEMIRMTLDRFGRIDVLVNNAGIQHVAPL
                     QEFPTAKWDAIIAINLSSAFHTTRLALPSMIDNKWGRIINIASAHGLVGSAFKSAYVA
                     AKHGMLGLTKVTALETAEMGVTCNAICPGYVYTPLVEMQIEGQAKAHGIPREQVIREV
                     LLAQQPNKRFATTDELGSIAVFLASDAAASITGTAIPVDGGWTAH"
     gene            8225..9445
                     /locus_tag="VFY74_02075"
     CDS             8225..9445
                     /locus_tag="VFY74_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5930527.1"
                     /translation="MIKIKNLTPREAVNQIRAKNNGQVALVLQGGGALGAYQGGVYQA
                     LHEAGIEPDWIIGTSIGAINASIITGNRPEDRLDRLREFWRRLERPNDWGVFPGWTGV
                     ADALSSWRTLTNGIPDFFEVYPRAFLGAHVPLGADHAGYYSTEALRKTLGELVHFSLI
                     NRKTPRLTVGAAHVRTSMMHYFDTRDMELTASHIMASGALPPAFPPVRIDGELYWDGG
                     ILSNTPTERIFEDYPRRNALIFAVHLWHPTGEEPGTIWQVLHRQKDIQYSSRISNYIV
                     RQRETHKLRHVISELLKFVPEEARADPRVKELAGYSCRTRMHVVRLLAPRLNNETHVK
                     DIDFSSAGICARWEAGYADTNRALETQAWLEECDPLEGVILHEPRAGAEPAAEAEMGG
                     AVTAPGDTPKLAAE"
     gene            9543..9701
                     /locus_tag="VFY74_02080"
     CDS             9543..9701
                     /locus_tag="VFY74_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930528.1"
                     /translation="MFWVRTPDQLHGGDIADALRKRSWLNALVAAGAAGAAGLLLFWV
                     STCSNGAF"
     gene            complement(10135..11370)
                     /locus_tag="VFY74_02085"
     CDS             complement(10135..11370)
                     /locus_tag="VFY74_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5930529.1"
                     /translation="MNEASSRIPVEQSEAQTHSEGLLLRSIVIGLTAFLTVVDLFATQ
                     AILPSLTRHYGVTPAAMGFAVNASTLGMAFSGLAIGYFSREIDRRKGILISLVLLAIP
                     TALLAHAPDITTFTILRVAQGLCMAAAFTLTLAYLGEHYSAADSASAFAAYITGNVAS
                     NLIGRLVSAAVADHFGLASNFYFFAVLNLLGAVLVYFTMERTPCMNCVSPAMGSHFTA
                     WITHLKNPALRASFAIGFCILFAFIGTFTFVNFVLVQPPLGVGAMTLGFVYFVFVPAM
                     ATTPLAGRAVSRFGTRPAFWAALGVAALGLPLLILPSLPAVLLGMVLVGAGTFFAQAC
                     ATGFVGRAATMERGAASGLYLASYFFGGLVGSAVLGPIFDMFGWKACVGGIAVALLIA
                     ALLAVFLKTAMTPAAVTRR"
     gene            complement(11443..11928)
                     /locus_tag="VFY74_02090"
     CDS             complement(11443..11928)
                     /locus_tag="VFY74_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5930530.1"
                     /translation="MLQSRTKGRFGRVALIAGIALGATAMTSGAFAGECPADQKKADA
                     REAVDLKPVGVTDTVIAMIDVGQEQGIEDRKFRMRKLVIEPGGIVPWHSHADRPAIIY
                     VVEGEIEEYASNCATPILHKAGEVVAETKDVSHWWKNSGDTTVVLLSSDLLKDPNDKN
                     M"
     gene            complement(12785..>14737)
                     /gene="uvrB"
                     /locus_tag="VFY74_02095"
     CDS             complement(12785..>14737)
                     /gene="uvrB"
                     /locus_tag="VFY74_02095"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5930531.1"
                     /translation="FPDNAVEYFVSYYDYYQPEAYVPRTDTYIEKESSINEQIDRMRH
                     SATRALLERDDVVIVASVSCIYGIGSVETYSAMTFTLKQGMRIDQRQLLADLVALQYK
                     RTSDFARGSFRVRGDTVDIFPAHYEDRSWRVHLFGDEIDKIEEFDPLTGHKQDDLEFI
                     KIYANSHYVTPRPTLVQAIKSIKSELKLRLDELNNHGRLLEAQRLEQRTTFDLEMMEA
                     TGSCAGIENYSRYLTGRRPGEPPPTLFEYVPDNALVFVDESHVTIPQIGGMFRGDYRR
                     KSTLAEYGFRLPSCMDNRPLRFEEWDAMRPQTIYVSATPAGWELQQTGGVFAEQVIRP
                     TGLVDPPVLVRPASTQVDDLIDEARKVVAQGYRVLVTTLTKRMAEDLTEYMHEQNIRV
                     RYMHSDVETLERIEIIRDLRLGAFDVLVGINLLREGLDIPECALVAILDADKEGFLRS
                     ETSLIQTIGRAARNIDGRVILYADGMTGSMERAIAETNRRREKQTAWNEANGITPESV
                     KKNIGDILASVYERDHVLVDAGVGFGEEDQALIGHNLESVIADMEKRMRAAAADLEFE
                     EAARLRDEVKRLRQMELAVANDPLARQGELERAAAETDITRPHKPSLEEMGPHNREMP
                     LAALAKARPRSKSGKPGTRAFKGKSR"
BASE COUNT         2728 a         4690 c         4707 g         2612 t
ORIGIN      
        1 gagggtgatt gaagggcaat ttaggggcgg gtgcgatcat ttttgattaa ggttaacaat
       61 cccttgaaga ctggcgcgcg acggcatcca ccgtttggga tctggtccaa gccgacgagc
      121 ctgtcgccca actctttgtc atttccggcc tgatctttgc ctgtttcaac cgatactgaa
      181 ggggtctgcg ttcatgatgg taagcgggag cggcctgacg tgggactgga caacagcaca
      241 attgggcgga acgggagcgc gcaagagagc cgggggcaat tccaaggggc gcgggagccg
      301 atgacgggcg cgcaggcctc ctatctcaag ttgctcgccg aagaaattca gcagccgtcg
      361 gcgttcgccg agaatttgag caagcgtgaa gcctcgcggc gcatcgacgc gctcgcggag
      421 agattgaggc tctgcaatct cccgccgcac acggactaga acacgcgctt cgatgggcct
      481 ctccggaccg gggaattggt ggcacagtga gaacggcagc cgccggtggc gtcttcggaa
      541 tgcagctctc ctcctcgcta ctggagcgtt gctccttgtc gattccggtc aagcaatggc
      601 cctaacgctg acatcctccg ctttcgcgcc gggcgaaaag atccccgcga aatacacgtg
      661 cgaaggcgac gatctctcgc cgccgctcgc cttcacaggc gtcccggacg gaaccaagag
      721 ccttgcgctt attgtcgacg atcccgatgt cccggacccg aaggcgccca agcgcatctg
      781 ggtgcactgg gtggttttca atcttccgcc ggatgcccaa ggcctgcccg aggacgtgag
      841 cggctcaggc ctgccaagcg gcgcggtgac ggggctgaac gaccggaaaa aggcctccta
      901 ccaaggaccc tgtccgccga tcggacggca tcgctatttt cacaagctct acgcgctcga
      961 cattacgctt ccggcgaaac agctcagcaa gtccgagctc gaagccgcca tgaagggtca
     1021 tgtgctggcg caagcggagc tgatcgggac ctatcagaag ggcgatccgt aagccggacg
     1081 cacggcaact cttgatccct tgacggacat gcaacgactg gtttgagtga cgccatggct
     1141 agacacccca tgactacggt tatccatccg atcgcacggg cggggatttt cgcggcagtt
     1201 ctgatcggac ttgcgccgaa cgcgtttgca gcctgcgcca gccccgccga gttcggccgg
     1261 tggctgcaag gcttcaagaa agaagccgtc gcgcaaggaa tttcaccgaa cacggtctct
     1321 tacgcgttgg atgggctcac ctacgatccc gccaccattg ccaaggatcg cgcgcagggc
     1381 gtgttcgcgc agaccttcct gcaattctcc gggcgcatgg tgtcgtccaa tcgccttcag
     1441 gtcggcgcgc agctgctaaa gaagcacgcc aacaccttca accagatcga gcagcaatac
     1501 ggcgtccccg gcccggtgct cgtcgccttc tgggggctcg agaccgattt cggcaaagtg
     1561 atggggaata tggagacgct ccggtcgctc gccacgctaa gcttcgattg ccggcgaccg
     1621 gacgagttcc gcgagcagct catgtttgcg ctgaaggtcg tcgagcgggg agacctctcg
     1681 ccgcaagaga tgcgcgggcc cgcgcatggc gaagtcggtc agttccagtt ccagccgaag
     1741 aactattacc aatatgccgt cgatttcgac ggcaacggca agcgcgacct gatccgaagc
     1801 gcgccggact cgctcgcctc ggccgccaac tacctgcaat cgatcgggtg gcaggcaggc
     1861 cagccctggc tcgagcaggt gcgcgtcccc gccgagctgc cgtgggatca ggccgatgtc
     1921 accatcagga agcctcgcgc gttctgggcc cagcacgggg tcacctattt gaacggcaag
     1981 ccgatccccg ccgacgatct ccaggccggg cttgtgctgc ccatggggcg gaacggcccg
     2041 gcgttcctcg cctacccgaa tttcgacgtc tatctcgaat ggaacaagtc gctggtctac
     2101 acgaccacgg cgggctacta cgcgacgcgt cttgccggcg ggcctgcgct tgcccgcggc
     2161 aacggtccgg tcgaaacgct cagtctcgcc gagaccaagc agctgcagca gcttctcgcc
     2221 aagcgcggct tcgaggtggg caagatcgac ggcgtgctcg gcgcggcgag ccgggacgcc
     2281 atccgcagca tgcaggtcaa atacggcatg ccggccgacg cctatcccac cgccgaattg
     2341 ctgagccgcc tgagcgcggg gcaatagcgc cctcgcttaa catccccctc gtggggaggt
     2401 cggcgcgccg caggactcag gccgccttca cgtcggccat cagcgcgaag aagccttcgg
     2461 tcagcgtggg atggatgaac atggcgtcct tgaacagcgt ccagggcgca tccgccatca
     2521 tcagcgccat caagatctgc accagctcgc cgccctcggg accgaggatc gtcgcgccga
     2581 gaatgcggtc gtcatcggca ttgatgacga tcttcatgag ccctttggtc tcgccccgct
     2641 cgatcgcgcg cgcgacgtag gccatcggca cgctgccgat cttcaggcga tagcctttcg
     2701 cgcgcgcctc ccgctcgctc agtccgacgc gcccgagctc ggggtcggtg tagagcgcat
     2761 agggcaccac gcgattgtcg atggtccggt tcttgccgtt tacgagattg tcggtgatga
     2821 cgagatagtc gtcgtaggac acatgggtga aggcgggacc gcctttgacg tcgccgagca
     2881 cccacacgcc ggggacgttg gtttcgagtc tgccgttgtg gcggatgaag ccggcctgat
     2941 cggtcgcgac gccggccttg tcgaggccga ggtcgtcgga attgggcgtc tgtccgaccg
     3001 ccacgagaag gtgcgagccg ctcagggtct cgatggtgcc gtccttgttc tcgatcgtca
     3061 cttccaaccc gcctggcgtc tttgcgatct tggcggcgcg ggcgccgaga cggaaattca
     3121 tgccttcctc ttcgagcgcc tctttcaggc tttgcgcgac ttcggcatcc tcgcgcggga
     3181 cgatctgctt ggcgagctcg acgaccgtga cctcgctgcc gaggcgccgg aacaattggc
     3241 cgaactcgag cccgagataa ttgccgccga gcgcgatgag acgagggggg acctccgtca
     3301 ggtccatgat gttgcgattg gtcaggtacg cgacctcatc gagccccgga aagggaatga
     3361 tgcggggccg cgtgcccgta ttgatgaaaa ttcggtcgct ggaaagcgtt tcgccattca
     3421 ccgcgacctc gtgcggaccg gtaaagcggc cacgcccgcg ataaagggtg agatttgggt
     3481 tggtcttgac ctgattttcg atgcccgagc ggaacgatcc gacgatttca ttcttgcgcg
     3541 cgacaatggc cgggaggtcg atcttgaccg gaccggcatg cacgccgaat tccgggccgc
     3601 gtcttacggc gcgcgcgatg cgagcgcttg cgatcatcgt cttggtgggc gtgcagccat
     3661 agttgatgca ggaacctccc agctggccct cttcgatcaa cgcgacctgc cagccaagct
     3721 tggcgaaatt ggcggccaga ggcacgcccc cctggcccga gccgatcaca atcgcatcgt
     3781 aggtcttcat gactttccct ccgcctatcg tcaccgggca gccgcgagct ggggctgcgc
     3841 gcggctccag atcagatggt cgagggaaag cgcgccgggt ccgcgtgcgg cgatgagcag
     3901 gagaagcgcg aaccagagga tgtgctcggg ccagccgtcg ggatagacga agaattgaat
     3961 gaccagggtc atcgccatga gagcgagcgc cgacaggcgc gaggcgagcc cgaccacgag
     4021 cagcaccgga aagacatgct cggcgatcgt tgccaagtag gcggcatgat cgggcaggag
     4081 cgggaccttg tattcctcgc gaaagagaaa gaacgtgttg tccttgattt gcaggaagcc
     4141 ctcgaccttg gtccggcccg agcgccaaaa gacgctggcg accgccaagc gcgcgaccag
     4201 ggccacgagg ctgtagggga tgcgctcggc caaacgccgg ccagcttcga gccagttgat
     4261 caaaattggc atcttaacct cctgtcccgg cgcaaagcgc cagcctatcg ggctaacctg
     4321 agccagctaa cccaatcggc gcgcttgaag gaaatgctca ctcatgatcg acaccatagc
     4381 cggtcgctat ttgacctttt ggacaataac ggccaaatca aaaagcctga gacttgcagg
     4441 acagcatcgc ttggcggtcg ctggcggcca tgttccgtcg gacgaccatc cgggcggaga
     4501 tggccgtacc ggacgtaggg aaaaatagaa tgaccatggc cttcgccacc atcgaagaag
     4561 cgttccgcta ttgccgcgac atggacgcct ccttgagcga aaggctggag accttcgcca
     4621 atgtggcgcg ggcgctgcgg ccggagatgc aagaggcaat cgaccggatg gtcgagcggc
     4681 tgaaggcgca taatgccggt gactccgccc ccaagccggg cgaagtcatg ccacccttca
     4741 cgctccccga cgaaacagga cggctcgtca gcctcgactc gcttctggcc gcggggcccg
     4801 tcgccatcac cttccatcgc ggccattggt gcccctattg ccgcatcaac acgcgggcgc
     4861 tctcggaagc gcaggaaaac atcaaggccg agggcgcgaa aatcgtggcg atcatgcccg
     4921 accgcgagca attcgccgct tcgttcaaat tggatgccga cctgcactat ccggttctga
     4981 ccgatatcga taacggctac gcgctctcga tcaacctcgc catttgggtg ggcgccgagc
     5041 tcgagcggct catgacggcc ggagggcgcg atctgccgcg ctatcagggt aacgacgcct
     5101 ggtttctgcc catcccggcg acgttcgtcc tcgcaagcga cggcaccgtg aggacgcgct
     5161 tcatcgatcc cgactatcgc cggcgcatga ccgtcgaaga ccttctcgcg gcgctgaaaa
     5221 acgctcggaa tcaggagaga atcggccaag gctcgtgatc ggcgcggctg caatcgccgc
     5281 taccgcttgg caacacccaa tggtatgaac gggtcgggga agatctcggg attgcgaacc
     5341 gcttctgcat tcggccgagc gtccggccgc agcggcccca ggacgttgaa tgacctcgaa
     5401 acccctttca cagcttctcg acgaggcctt tgtccaatgc cgggacatgg atgcgtcctt
     5461 ggccgagaga ttgcaggcct tcgccgatgc ggtgaggcgg ttcggtccgc attttcagga
     5521 tgccgtcgat ggactcgtca acaggctgaa gcagtttgag gtcggcgaca atgcgccgaa
     5581 gcccggcgag gtgatgccga actttgttct gcccgatgag aacggccaac tggtcagcct
     5641 cgacagcctg ttgcagaaag gacccgtcgc cgtcaccttc catcgcggtc attggtgccc
     5701 ctattgccgc atcaacacgc gcgtgttggc cgaggcgcag caccaaatcg ccgccgaagg
     5761 cggacaggtc gccgcgatca tgcccgaccg tgagcacttt acgaccgagc tcaaggccga
     5821 gtcggaggcg ccgtttccga tcctcaccga tttcgacaac ggctatgcgc tgtcgctcaa
     5881 tctcgccttc tgggtcggcc aggaaatgca ggagctcatg accatggccg ggtgggacgt
     5941 gtctcgctcg cagggcaccg atacctggct tctgccgatc cccgccacct tcgtcgtcgg
     6001 taccgacggc gaggtgaaag cgcggttcct cgatcccgac taccgcaagc gcatgacgat
     6061 cgaggatttg ttgtccgcgc tccggcaagc gcgcgagtcc gctcactagg cggcggaccg
     6121 cgtccaatcg taagcgcgca gcatcttcat cagggccgag gctacggccg tgcgctgccg
     6181 ccctgccacg ccgtaaagga agatcgtgcg ctggagctcg aggccctcga cggcaatacg
     6241 gacgagggtg gccggacctt gcgtactgcg cggcaccaag gccgttccga cgccggcttc
     6301 gagcagcgag agaaggtcgg cctcggcgcc tacctcgtga aagcgcgtca cgtcgaaatc
     6361 ctgcgcgcgc aggatttcga gcagcgcctc cgtcgtctcg caataggtgc ggaccatcag
     6421 ccgctcttgc cggagatcgt cgatggaaac ggtggagcgc ccagccaggg cgtgggtgct
     6481 ctgcaccagg agctgaaagt cttcggtgaa gagcggccag cgatcgagcc gctcccactc
     6541 gtcgccgatc tgggccgcga gaccgagctc gacctcgccg cgcttcagat gttcgagcac
     6601 ctctgttccg ttgccccgca ggatcttgag gtccaggcct tcgaacagct tgcgaagctc
     6661 gaccacgtgg ggcgtgacca gcgacgggtc tatggtgcgc gacacggcga gcctgagccc
     6721 gccgacctcg cctttcttga tcgccgaagc gagagcgcgc gcgctgatgg cgctctcata
     6781 gcactgcttc aacagcggca gcatgcgctg accaagttcg gtgagctggg cctgcggccg
     6841 ctcgcgccgg aacagatcgc cgccgagctc gccctccaat tggcggatcg cgcgggtgag
     6901 cgacggctgg gcgacgtggc attcctccgc ggcacgggtg aaattcagga cgcgcgccac
     6961 cgccaggaaa tagcggacct gatgcatttc catgggagct gcgccccgcc cgtcctacca
     7021 cgtacccaaa ggccatccgc cccggcgatg gctgcagcca gccgcagtca gacaggcttc
     7081 ggcctgtcgc ctcgccttgc caatggtcat tggtccggaa atgcttggtt cgcgccttcg
     7141 aagatcggtt ttgcgcttgc gagcgctcaa cggcaagctg atttgaccca tttgctcggt
     7201 ttttgacaag cgagtttgtg gcgcgttcag cccctttccg gcctttggcg gcacctcggg
     7261 caaccatagc gctcccctat cgactcctta gccaacgggc atttcccgcg ccgttttgct
     7321 acgatcatga tcggcggaaa ggatgcactg tcccgccgct tggaaagcgg gcgccggata
     7381 cccacagagg agtggagcgc aatggcgacg tccttgaggg acaaggtgtc cctggttacc
     7441 ggctcgacca gcgggatcgg gcttggcatc gcccgcgccc ttgccgcagc gggttccgcc
     7501 gtcgtgctca acggtttcgg caagcccgag gaaatcaagg tcacccaaga tgcgatcgaa
     7561 gccgatttcg gcgtgccggt cgcctattcc gacgccgaca tgtcgaagcc ggactccatt
     7621 gccgaaatga tccggatgac cctcgacagg ttcggcagga tcgacgtgct cgtcaacaat
     7681 gccggcattc agcacgtggc gccgttgcag gagttcccca ccgccaagtg ggacgccatc
     7741 atcgccatca atctcagctc ggcgtttcac accacgcggc tcgcgcttcc gagcatgatc
     7801 gacaacaaat ggggccgcat catcaatatc gcctcggccc atggacttgt cggctcggcc
     7861 ttcaagtcgg cctacgtcgc cgccaagcac ggcatgctgg gcttgaccaa ggtcaccgcg
     7921 ctcgaaaccg cggagatggg cgtgacctgc aacgccatct gcccgggcta tgtctacacg
     7981 ccgctcgttg aaatgcagat cgaaggacag gccaaggccc acggcatccc gcgcgagcag
     8041 gtgatccgcg aggtccttct ggcgcaacaa ccgaacaaac gcttcgccac caccgacgag
     8101 ctcggcagca tcgcggtgtt cctcgcctcc gacgctgccg cctccatcac cgggaccgcc
     8161 attccggtgg acggcggctg gaccgcgcat tgagcggaag cgccaacacg attgggagcc
     8221 ggccatgatc aagatcaaga acctcacgcc ccgcgaagcc gtcaaccaga tccgcgccaa
     8281 gaacaatggt caggtcgcgc tcgtgctcca aggcgggggc gcgctcggcg cttatcaggg
     8341 cggcgtgtat caggccctgc atgaggcagg catcgagccc gactggatca tcggcacctc
     8401 gatcggtgcc atcaacgcca gcatcatcac cggcaacagg cccgaagacc gcctggatcg
     8461 gctgcgggag ttctggcgcc gtttggaacg cccgaacgac tggggcgtgt tcccgggatg
     8521 gaccggggtg gccgatgcgc tgtcgtcatg gcgcacgctc acgaatggca ttcccgactt
     8581 cttcgaggtg tacccgcgcg ccttcctcgg cgcgcatgtg ccgctcggcg ccgaccatgc
     8641 cggctattac tcgaccgaag ctctccgcaa gacattgggg gagctcgtcc acttctcgct
     8701 gatcaatcgc aagactccgc ggctgaccgt gggcgcggcc catgtgcgga ccagcatgat
     8761 gcactacttc gatacgcgcg acatggagct caccgcgagc cacatcatgg cgtccggcgc
     8821 gctcccgccg gccttcccgc ccgtgcgcat cgacggcgag ctctattggg acggcggcat
     8881 cctctccaac acgccgaccg aacgcatctt cgaggattat ccgcgtcgca acgcgctcat
     8941 cttcgcggtg catctttggc acccgaccgg cgaggagccc ggaacgatct ggcaggtcct
     9001 gcaccgccaa aaagacattc agtattcgag ccggatctcc aactacatcg tgcgccagcg
     9061 cgagacgcac aagcttcgcc atgtcatctc cgagcttctg aaattcgtcc ccgaggaggc
     9121 gagggccgac ccgcgggtga aagagcttgc cggctattcc tgccgtacgc gcatgcatgt
     9181 ggtgcggctc ctcgcgccgc gtctcaacaa cgaaacgcac gtcaaggaca tcgacttctc
     9241 gtccgcggga atctgcgcgc gctgggaagc gggctacgcc gacaccaacc gcgcgctgga
     9301 gactcaggcg tggctcgagg aatgcgatcc gctcgagggc gtgatcctgc acgagccgcg
     9361 ggcgggcgcc gagcccgctg ccgaagcgga gatgggcggg gccgtcaccg cacccggcga
     9421 tacgccgaag cttgccgccg aataggagga gaggcgactc ccatggatgc gtgcacgggc
     9481 ataacggcga tccagcttct tgccgcggtg ttcaccttcc tcgggcggtg gtgtcgctcg
     9541 atgtgttctg ggtccggacg cccgatcaac ttcacggtgg cgacatcgcc gacgcgctca
     9601 ggaagcggag ctggctcaat gcactcgtcg ccgccggcgc ggcgggcgcg gccggtctgt
     9661 tgctcttctg ggtcagcacc tgctcgaatg gtgcgttctg aacccggtct ggcgtccagc
     9721 gttcgcgaca gcaggaaaag aaaaagcccg gcttcgccgc cgggcttctt acgcgatacg
     9781 gagactctct tgcaacgcaa caaacaagca accccgagcc ttcacccctc ttgaaccgcc
     9841 gggatgcgag acgcgcgggc gacgaggggg cgctgatcca gcggccttcc tatttgactg
     9901 aggttggcgg caactcggac gtgtagacct gccgcgagtg gtggaagcag ccgcccagcg
     9961 cgattgacgc tgcgatgacg acgagagcaa cgaagaggcg catgactgat ccctctgact
    10021 ttgacttacg ctcgacatga gttacgcgag acttgccgcc aattgagggt gctccgcttg
    10081 aaaaagcgtt aatgcgcgag tcgctttggc accgaccgag aaaacggcct cgggctagcg
    10141 acgtgtgacg gccgcgggtg tcatcgccgt cttgaggaag acggcgagca gggcggcgat
    10201 gaggagcgca acggcaatgc cgccgacgca ggccttccag ccgaacatgt cgaagatcgg
    10261 cccgagcacg gcgctgccga ccaggccgcc gaagaaatag ctggcgagat agaggccgct
    10321 cgccgcgccg cgctccattg tcgccgcgcg gccgacgaac cccgtggcgc aggcctgggc
    10381 gaaaaacgtc ccggctccca ccaacaccat gccgagcagc acggccggca ggcttggcag
    10441 gatgagcagc gggagcccga gagcggccac gccgagcgcc gcccagaacg cgggcctggt
    10501 gccgaagcgg ctgacggcgc gcccggccag tggcgtggtc gccatggcag gaacgaacac
    10561 gaaatagacg aagccgagcg tcatggcgcc aacgccgaga ggcggttgca ccagaacgaa
    10621 attgacgaag gtgaacgtgc cgatgaaggc aaacaggatg caaaagccga tggcgaaact
    10681 cgcgcgcaaa gcggggttct tcagatgcgt gatccaggcg gtgaaatgcg agcccatcgc
    10741 cgggctcacg caattcatgc atggcgttct ttccatggtg aaatagacca gcaccgcacc
    10801 gagcagattg agcacggcga agaaatagaa attgctggcg agcccgaaat ggtcggcgac
    10861 ggccgccgag accaatcggc cgatgaggtt gctggcgaca ttgccggtga tgtaggcggc
    10921 aaacgcgctc gccgaatcgg cggcgctgta atgctcgccg agataggcga gcgtgagcgt
    10981 gaaggcggcc gccatgcaca gcccctgcgc cacccgcaag attgtgaacg tcgtgatgtc
    11041 cggcgcgtgc gcaagcagcg cggtcgggat ggcgagcagc acgaggctga tcaggatccc
    11101 tttcctgcga tcgatctcgc ggctgaaata gccgattgcc aagccggaaa atgccatgcc
    11161 gagcgtgctc gcattgacgg caaaacccat ggcggcgggc gtgacgccgt aatggcgcgt
    11221 gagcgaggga aggatggctt gggtcgcgaa cagatcgacg acggtgagga aggccgtcag
    11281 accgatgacg atggaacgga ggagcaagcc ctcggaatgc gtctgcgctt cggattgttc
    11341 gaccggaatc ctggaagacg cttcgttcat ggcaggctcg tcaaaacgaa agagtggatg
    11401 tgatgccctg ggggaggagg catcacatcc gatgggggga aattacatgt tcttgtcgtt
    11461 cgggtccttc agcaggtcgg aggagagcag cacgaccgtc gtgtcgcctg agttcttcca
    11521 ccaatgcgag acgtccttgg tttcggcgac gacctcgccg gccttgtgca ggatcggcgt
    11581 ggcgcagttg ctggcatatt cttcaatctc gccttcgacg acgtagatga tcgccgggcg
    11641 gtcggcatgg ctatgccacg gcacgattcc gccgggctcg atcacgagct tgcgcatgcg
    11701 gaacttgcga tcctcgattc cttgctcctg cccgacgtcg atcatggcga tgacggtgtc
    11761 ggtgacgccg accggtttca ggtccaccgc ctcgcgcgcg tcggctttct tctgatctgc
    11821 ggggcattcg ccggcaaagg cgcccgaggt cattgcggtc gctccgagcg caataccggc
    11881 gattaaagca acacggccga accggccttt tgttctcgat tgcaacatct ccgttcactc
    11941 cttctggcta gtgctccggc cgaaaggaac ggccgcggcg ataggcgaag gatcgcccga
    12001 agccgcctcg aacggaaatg ccagttggct tccgtctcga tagccgaccg ctatccggtg
    12061 cacggaatgc acccgttcca tagcgataag gtattggccg atcagggcta tccaacacta
    12121 ccttcagggc gttccggacg gacatccatg gccatccgtt cggagcgtcc gaccccaaag
    12181 gccatctgaa ggagacttga aatgaagcag ctcttgatcg ttctgaccct ggtcgtcgcc
    12241 tcagccgcgc ccgccatggc cgatggtttt ccgaatccct accagaccag ccgctaggtg
    12301 attgggaggc tgtcaatcct gatctctcga aaggagaccc caaatgaaga ccctttttct
    12361 cgctcttgct ctcgtcgtcg cgaccgcggc gccaagcatg gctgtcgaat tcaacaacag
    12421 cactgtgcag ggtggtttcg gccgttaagc cgagtgggtc acgccgcgtg acccgcgcca
    12481 ttccgaagaa atcgcgggcg ctctcgatga ggcgcccgca attttttgcg ctctctcttc
    12541 ccctctcccc gcgggagagg gtggcgaagc cgagcgcaag cgaggctgag ccgggtgagg
    12601 gcgccggtgt ttcaggctgc aaccgtccgg ccgcttgaag cgcaggcccc ccacccggcg
    12661 cgcaaaggcg cgccgacctc cccacgacct gcctgcgcga agccggagct tcgcttcggc
    12721 gaaggcaggg gggaggttat gaagctagtc ggtaactcag cgcaaagacg gcgatccgcg
    12781 ggcgctagcg gcttttgccc ttgaaggcgc gggtgccggg cttgccggat ttcgagcggg
    12841 gccgcgcctt cgccaaggcg gcgagcggca tctcgcggtt gtgcgggccc atttcctcga
    12901 gcgagggctt gtgcggccgg gtgatatcgg tctcggccgc cgcgcgctcg agctccccct
    12961 gccgggctag cgggtcgttg gcgacggcga gctccatctg gcgcaagcgc ttgacttcgt
    13021 ccctgagccg cgccgcctcc tcgaattcga ggtcggcggc ggccgcgcgc atccgcttct
    13081 ccatgtcggc gatgacgctt tcaagattgt gcccgatcag cgcctggtcc tcctcgccga
    13141 accccacccc ggcatcgacg agcacatggt cgcgctcgta gacgctggcg agaatgtcgc
    13201 cgatattctt cttcacgctc tccggcgtga ttccgttcgc ctcgttccac gccgtctgct
    13261 tctcgcgccg gcggtttgtc tcggcgatgg cgcgttccat cgagccggtc atgccgtcgg
    13321 catagaggat cacgcgcccg tcgatattgc gcgcggcgcg gccgatggtc tggatcagag
    13381 aggtctccga gcgcaggaac ccctccttgt cggcatcgag aatggcgacc agcgcgcatt
    13441 cgggaatgtc gaggccctcg cgcaacagat tgatgccgac cagaacgtcg aacgcgccca
    13501 gccgcaaatc gcggatgatc tcgatgcgct cgagcgtctc cacgtcggaa tgcatgtagc
    13561 gcacgcggat attctgctca tgcatgtact cggtcaggtc ctcggccatg cgcttggtga
    13621 gcgtggtgac gagcacgcga tagccttgcg ccaccacttt gcgcgcctcg tcgatcaggt
    13681 cgtcgacctg ggtcgaggcc gggcgcacca gcacgggcgg gtcgacgagg cccgtcgggc
    13741 gaatcacctg ctcggcgaac acgccgccgg tctgctgcaa ttcccagccg gcgggcgttg
    13801 ccgagacata gatcgtctgc ggccgcatcg cgtcccactc ctcgaagcgg agcggccggt
    13861 tgtccatgca ggaggggagc ctgaagccgt actcggcgag ggtggacttt cgccggtagt
    13921 cgccgcggaa catgccgccg atctgcggaa tcgtgacatg gctctcatcg acaaacacga
    13981 gcgcgttgtc gggcacgtat tcgaacagcg tcggcggcgg ctcgccgggg cggcggccgg
    14041 tgaggtagcg cgaatagttc tcgatgccgg cgcagcttcc ggtcgcctcc atcatttcga
    14101 ggtcgaaggt ggtgcgctgc tccagccgct gcgcttccag caggcggccg tggttgttga
    14161 gctcgtcgag ccgcagtttc agttccgact tgatcgactt gatcgcctgc accagcgtcg
    14221 ggcgcggcgt cacatagtgc gagttggcgt agatcttgat gaattcgaga tcgtcctgct
    14281 tgtggccggt gagcggatcg aactcctcga tcttgtcgat ctcgtcgccg aacagatgca
    14341 cgcgccagga acggtcctcg tagtgggccg ggaaaatgtc gaccgtatcg ccgcgcaccc
    14401 ggaacgagcc gcgcgcgaaa tcgctggtgc gtttgtattg cagagcgaca agatcggcga
    14461 gcaactggcg ctggtcgatc cgcatgccct gcttgagcgt gaacgtcatc gccgagtagg
    14521 tctcgaccga tccgatgccg tagatgcacg acaccgaagc cacgatcacc acgtcgtcgc
    14581 gctccaagag cgcgcgcgtc gcggagtggc gcatgcggtc gatctgctcg ttgatcgacg
    14641 attctttctc gatatacgtg tcggtgcgcg ggacgtacgc ttccggctgg tagtagtcgt
    14701 agtaggagac gaaatattcg accgcgttgt cggggaa
//
LOCUS       DASUQP010000046         8268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_19851,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000046 DASUQP010000000
VERSION     DASUQP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19851"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFY74_02100"
     CDS             complement(<1..1048)
                     /locus_tag="VFY74_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5930532.1"
                     /translation="MRDILQGNRRGKLIDWLKIDSWIDSGLYSAWSGFRDWWSGYSSF
                     FNRFEVNGFVRGLNELACEALTLSVGGLLILASFALPAFETAQGKMNLADEYSVTFLD
                     RFGNEIGKRGLLRDDSVPLEEIPDVMIKATLATEDRRFFEHFGIDVMGTFRALAANAR
                     NDQVVQGGSSITQQLAKNMFLTPERSLSRKIREALIAIYLENRYTKPELLKLYFDRAY
                     LGGGSYGVEAAAQYYFNKSIREVNLAEAAMLAGMFKAPTRYAPHVNLAASRARANEVL
                     TNMVEAGFMSEGQVYGARMNPSKIVERGDGKTPDYFLDWAFEEVQRLLLGKEDHIVIA
                     RTTVDLGLQRAAEEA"
     gene            complement(1291..1581)
                     /locus_tag="VFY74_02105"
     CDS             complement(1291..1581)
                     /locus_tag="VFY74_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930533.1"
                     /translation="MSRVKLWAFAGFLIAMPASGCSLSNPAPRDFTLVDQRPAKAEEA
                     SNTLEQAEAACKEETKRKGIASVIGILSRLRKGDADEDYVACMKARGYEVKP"
     gene            complement(1670..2374)
                     /locus_tag="VFY74_02110"
     CDS             complement(1670..2374)
                     /locus_tag="VFY74_02110"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5930534.1"
                     /translation="MMATPEYQSLLLRKFIRRTALSLLFVGAILFGAAGTLNWPQAWL
                     YLALASVISFGAGSWLARNDPALLSERLGPLIQREQKRWDKALMAVMLSLWVGWLILI
                     GLDRRYHWSDVPIALQGAGLALVVLGSYLVWLTLKANTYAAPVVKIQKARGHAVATSG
                     PYAYVRHPMYAGALLFLAGMPLLLGSWWGLAAGLALVLIIAVRAVFEERTLAAELEGY
                     AEYAARVRYRFVPYLW"
     gene            2520..2873
                     /locus_tag="VFY74_02115"
     CDS             2520..2873
                     /locus_tag="VFY74_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930535.1"
                     /translation="MPKIALIVAVLLSAAVLASSPVDAAAPSSDAEAPAPSGPPKGYT
                     CHDKAFTGSGPGFKSSQDESEGAAIKDWLGKATAIFADADWNTAKGPVMECVKQGLYS
                     KCFATGVPCHPKPKE"
     gene            2870..3145
                     /locus_tag="VFY74_02120"
     CDS             2870..3145
                     /locus_tag="VFY74_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930536.1"
                     /translation="MSDPILRAVLGTTILIALVGGAEAAGTAEQAPAPKCLKAEINPI
                     TGHILCIDPLGAPVEPLPDAAKPPCRPEDARGQWSYGPACSPEPGGD"
     gene            3443..3865
                     /locus_tag="VFY74_02125"
     CDS             3443..3865
                     /locus_tag="VFY74_02125"
                     /inference="COORDINATES: protein motif:HMM:NF022810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEX5930537.1"
                     /translation="MTAVQSETLRNSIHRALTGSTDLAALLGGVRIYDATPQTATFPF
                     ITLGQSTLRDWSTGSEDRAHQSLTVHVWSPFGGKKHVQDAIKAVRTALNDRPLPAGRQ
                     LVTLHHEFSEARIDPDDDTFHGIIRYRATALPARAVAA"
     gene            3966..4436
                     /locus_tag="VFY74_02130"
     CDS             3966..4436
                     /locus_tag="VFY74_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5930538.1"
                     /translation="MTLWAKLKDLVDRLGGDEGHAPNLREEELRLAAAALLVHASAID
                     GDVDSAERRKLKALLQARFDLDGDALRKLLGEADAREVESVDLYQFTSVLCRELDQDG
                     RKRIVEMLWEVAMADGVIHEFEANLVWRAAELLGVSTRDRVTLRKAVESRNARF"
     gene            4603..4962
                     /locus_tag="VFY74_02135"
     CDS             4603..4962
                     /locus_tag="VFY74_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930539.1"
                     /translation="MRRSGGTLYGPAQEGYWDRTMWRVLKPAIVAALAAAFLATAGHA
                     TEPGCVEWSRAGPIIAENGLVPANVIFKEVQKRAGGKIVSQALCNFGGQFVYRLVVLG
                     PTGEVANVTVDARTGQF"
     gene            4997..5668
                     /locus_tag="VFY74_02140"
     CDS             4997..5668
                     /locus_tag="VFY74_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5930540.1"
                     /translation="MRLLVVEDDPDLNRQLVAALSDAGYAVDSAFDGEEGHFLGDTEP
                     YDAVVLDLGLPKKDGLSVLEQWRRDDRKMLVLILTARERWSDKVAGMDAGADDYVAKP
                     FHMEEVLARLRALLRRAAGHARNEMECGPLRLDTKTARVTVDGRAVKLTSHEYRVLAY
                     LMLHKARVVSRTELVEHLYEQDFDRDSNTVEVFIGRLRKKLPGDLIKTVRGLGYRLSD
                     AGDEA"
     gene            5658..7022
                     /locus_tag="VFY74_02145"
     CDS             5658..7022
                     /locus_tag="VFY74_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5930541.1"
                     /translation="MRHNSLAFRLFASAAAWTLVVLPVTAILLVSLYREALERNFDTR
                     LNVYLTSLVASSTQEGAATPKEPADFGQPIFGIPFSGWYWQIKPLNRAARPDFVSDSL
                     LDQQLKLPSQSGAAPDASLTRRAYIEGPNDQRLRIVEREIRQRAPGSAPYSYAVAGDS
                     SEIERDLSEFTMMLIAALAVAGLGLVAATFFQVRFGLSPLRAIRQGLTAIRSGEAEKL
                     EGELPLEIKPLQQELNALIHSNREIVERARTHVGNLAHALKTPLSVISNEARTQEGVL
                     AAKVIEQAEIMRDQITHHLDRARVAARSSVIGDVTDVGVVLEALKRTLDRIYEERGLE
                     LELAATPGLKFQGERQDFAEMVGNLLDNACKWARSKVRASAERNGADARFTVIVDDDG
                     PGLTGAERASAVKRGQRLDETKPGSGLGLSIVADLAHLYKGRFELEPSPQGGLRARLE
                     LPAA"
     gene            7429..7893
                     /locus_tag="VFY74_02150"
     CDS             7429..7893
                     /locus_tag="VFY74_02150"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX5930542.1"
                     /translation="MRRAPILSILVISTVLAACGPGNKQGTGTIVGAVAGGAAGGAIA
                     GSNNRAGGVAIGAVVGGILGNQIGRSLDEEDRRLAAAAEYQALEYGQAGASTPWDNPQ
                     TQHHGQIVPGKPYQQGTQYCRPYTHTIYIGGQPQTARGTACRQPDGTWQTVG"
     gene            8034..>8268
                     /gene="ccmI"
                     /locus_tag="VFY74_02155"
     CDS             8034..>8268
                     /gene="ccmI"
                     /locus_tag="VFY74_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX5930543.1"
                     /translation="MFVWLVLAGLTAIVLFVLLRPLMAAGMESRAAESFDAAVYRDQL
                     KEIESDRARGLIGDREAEAARIEIARRLLAADEK"
BASE COUNT         1494 a         2661 c         2661 g         1452 t
ORIGIN      
        1 aagcctcctc agccgccctt tgcaggccga gatcgacggt ggtcctcgca atgacgatgt
       61 ggtcctcctt gcccagcaac agccgctgaa cctcttcgaa ggcccaatcg aggaagtagt
      121 cgggcgtctt gccgtcgccg cgctcgacga tcttggaggg gttcatgcgg gcgccataga
      181 cctggccttc gctcatgaat ccggcctcga ccatattggt cagcacctcg ttggcgcggg
      241 cgcgcgaggc cgccaggttg acgtgaggcg cgtagcgggt aggcgccttg aacatgccgg
      301 cgagcatggc cgcctcggcc aggttcacct cgcggatgga cttgttgaaa tagtactgag
      361 ccgccgcctc caccccgtac gagccgccgc cgagataggc gcgatcgaaa tagagcttca
      421 acagctcggg cttcgtataa cgattctcca aatagatggc gatgagcgcc tccctgattt
      481 ttctgctcaa ggagcgctca ggcgtaagga acatattctt ggcgagctgc tgggttatcg
      541 agctgccgcc ctgcacgacc tgatcgttgc gggcattggc ggcaagtgcc ctgaacgtgc
      601 ccatgacgtc gatgccgaaa tgctcgaaga aacgtctgtc ctcggtcgcg agcgtcgcct
      661 tgatcatcac gtcggggatt tcttccagcg gcaccgagtc gtcgcgcaac aggccgcgct
      721 tgccgatctc gttgccgaag cgatcgagga aggtcacgct gtactcgtcg gcaagattca
      781 tcttgccttg cgcggtctcg aacgcgggaa gcgcgaagct ggcaaggatg aggagaccgc
      841 cgacgctgag ggtcagcgcc tcgcaggcaa gctcattgag gccgcggacg aaaccattga
      901 cctcgaagcg gttgaagaac gaggaatagc cgctccacca gtcgcgaaag ccgctccagg
      961 cgctatagag gcctgaatcg atccaggaat cgatcttgag ccaatcgatc agcttgccgc
     1021 gccgattgcc ctggagaata tcccgcacct tgccccgacc tttcgcatac aaaaacgtcc
     1081 cggtagatcg accgtcctca atcgaccgcc cggacgcgtc cctttgccca tccattatac
     1141 cgcgattgag gcggtttatg gagaggttgc aacgggtagg cgcgcgcggt cgactcggtc
     1201 gaccttggga caatgtttgc tctcatggag ggttcccgga gcccattcga gaggtgtcgc
     1261 cgggccttga accgcgcctc gcagcctggc tcatggtttg acttcatagc cccgcgcctt
     1321 catgcaggcg acgtagtcct cgtcggcgtc acccttgcgc aggcgggaca ggatgccgat
     1381 gaccgaggca attcccttgc gcttggtctc ctccttgcac gccgcctcgg cctgttccag
     1441 cgtgttgctc gcctcctcgg cctttgccgg ccgctgatcg accagggtga agtcgcgggg
     1501 tgccgggttc gacaggctgc agcccgacgc tggcatggcg atcaggaagc ccgcaaacgc
     1561 ccagagtttg accctcgaca tcccgtctct cgacccgttg ctccccaatg atcgcacaag
     1621 gcgcggagcc tccgaaaccc tgtttgccgc cggccgctgc gatcgagcgc taccataggt
     1681 acggaacgaa ccgatagcgg acgcgggcgg catattcagc atagccttcg agctcggccg
     1741 ccagcgttcg ctcctcgaac acggctctca ccgcgatgat aaggacaagg gcgagccccg
     1801 cggcaagtcc ccaccatgag ccgagcaaaa gcggcatgcc ggcgaggaac aagagggcgc
     1861 cagcatacat ggggtgtctt acgtaagcgt aaggcccgct cgtcgccacg gcatggcctc
     1921 gcgccttttg aatctttacg accggcgccg cgtaggtgtt cgccttgagc gtcagccaga
     1981 cgagatatga cccgaggacg acgagcgcga gaccggcgcc ctggagggca atcggaacat
     2041 cggaccagtg gtagcgccgg tcgagcccga tcaggatgag ccatccgacc cagagcgata
     2101 gcatgacggc catcaaggcc ttgtcccaac gcttctgctc ccgctggatg agcgggccca
     2161 agcgctcgct gagcaaagcg ggatcgttgc gcgcaagcca ggaaccggca ccgaaactga
     2221 tgacggaagc gagggcaaga tagagccagg cttgtggcca gttgagcgta cccgccgcgc
     2281 cgaacagaat agcgccgaca aacaggagcg atagggccgt gcggcggatg aacttccgca
     2341 gcagaaggct ttggtactcc ggggtggcca tcatggcatc aagagccggt tcgcgttcac
     2401 gaaggcgtga atgcagacgc ctgcgcttgc aagttctccc cctggtccct ggcgttggca
     2461 tagactgccg gcgcgccgca ctcaagaaga aggatccgcc gggctttggc ggaggtcaga
     2521 tgccgaagat cgccttgatc gttgccgtgc ttctcagcgc cgcagtgctc gcctcttcgc
     2581 ctgtcgacgc ggccgcgccc tcctctgatg ccgaggcccc ggccccctcc ggtccgccca
     2641 agggctacac gtgtcacgac aaggcgttca cgggcagcgg gcccggcttc aagagctcgc
     2701 aggacgagtc ggagggggct gccatcaagg attggcttgg caaggctaca gcgatctttg
     2761 ccgatgccga ctggaacacg gcgaaaggtc cggtcatgga atgcgtcaag cagggcctct
     2821 acagcaaatg cttcgccacg ggcgtgcctt gccatccgaa gcccaaggaa tgagcgaccc
     2881 catcttgcgc gcggtgttgg gcaccacgat cctgatcgcg cttgtcggcg gggccgaggc
     2941 cgccgggaca gccgaacagg cgccagctcc caaatgcctc aaggcggaga tcaatcccat
     3001 caccggacat atcctctgca tcgatccttt gggcgccccg gtcgagccgc tccctgacgc
     3061 ggcgaagccg ccatgccggc ctgaggacgc ccgaggtcag tggagctatg ggccggcttg
     3121 ctcgcccgag cccggcgggg attagacagt cacgtcattc cggcgcaagc cggaatccag
     3181 ggcggctggc acagtgctgg tggcccttgg ataccggctt tcgccggtat gacggagaag
     3241 gccggcatgg cgggcatccc cccgccgttc tagcgggttt ccgctcgttt accgcgcgtt
     3301 aagcatgcgg ccggaaactg ggcaaaacgg cttcaaatcg tccgggaagc gcggtctcat
     3361 cgcggattgg tgattgcgtg gtttgaacgg cgtgacgcgg caccccatat ccttggttaa
     3421 cagcctatcc gaggcatccg ccatgacggc agttcagagt gagacgcttc gcaacagcat
     3481 ccaccgtgcg ctcacgggat cgactgacct tgcggccctg ctcgggggtg tcaggattta
     3541 tgacgcgacc ccgcaaaccg cgacctttcc gttcattacc ctcggccaaa gcaccctccg
     3601 cgattggagt accggaagcg aggatcgcgc ccaccagagc ctgacggtgc atgtctggtc
     3661 gccgttcggc ggcaagaagc atgtgcagga tgcgatcaag gccgtcagga ctgcgctgaa
     3721 cgatcggccg ctgccggccg gccgtcaact cgtcacgctc caccacgaat tttctgaggc
     3781 gaggatcgat cccgatgacg atacctttca cgggatcatc cgctaccgcg ccacggctct
     3841 gccggcgcgc gccgttgccg catagtttcc cattcggcca agggttgctc tcatcgcaag
     3901 agggtcgcgc tttcgaccct cgctgcgcta tatgacgcgc gcagggatga ggcgagggcg
     3961 cgcgtatgac gctgtgggcc aaactcaagg atctcgtcga tcggctcggc ggggacgagg
     4021 gacatgcgcc gaatttgcgc gaggaagagc tgcggctggc cgcggcggcg ctcctcgtgc
     4081 atgcgtccgc cattgatggc gatgtcgatt ccgccgaacg ccgtaagctc aaggccctgt
     4141 tgcaagcgcg cttcgatctc gatggcgatg cgctccgcaa gcttctcggc gaggccgatg
     4201 cccgcgaggt cgagtccgtc gatctctacc agttcaccag cgtgctctgt cgcgagctcg
     4261 accaggacgg ccgcaagcgc attgtcgaga tgctgtggga agtggcgatg gccgacggcg
     4321 tcatccacga attcgaggcc aatctcgtct ggcgggcggc ggagctcctc ggggtctcga
     4381 cccgtgaccg ggtgacgcta cggaaagcgg tggagagccg taacgcccgc ttctgacgga
     4441 gcgcggcatg agcgtcggcg gccgatctgc tactttgcgt cgggcccggg aaacgtgccg
     4501 ccgacgacat ccggccacga ccaactcgct cacgccgttc tcgcggagca gcagcaccta
     4561 aacgcagcgt ttcgtgaccg gggaggggga ttccaagcgt tcatgcgccg ttcagggggc
     4621 acgctttacg gtcccgccca agaaggatat tgggaccgaa ccatgtggcg ggtactgaag
     4681 ccagcgatcg tggcggccct cgcggctgcc ttccttgcga cggcgggaca cgcgacggag
     4741 cctggctgcg tggaatggag ccgcgccggc cccatcatcg ccgagaacgg tctcgtgccc
     4801 gccaatgtca tcttcaagga ggtgcagaag cgcgccggcg gcaagatcgt cagtcaggcg
     4861 ctctgcaatt tcggcggaca gttcgtctat cggctcgtcg tgctcggacc gacgggcgag
     4921 gtcgccaacg tcaccgttga cgctcggacc ggccagtttt aggtacggcc caagctcgac
     4981 acggaggaat agcgctttgc ggcttctcgt cgtcgaggac gatccagacc tcaatcgaca
     5041 gctggtcgct gcgctcagcg atgcgggcta cgccgtcgat agcgccttcg acggcgaaga
     5101 gggacacttt ctcggcgata ccgaacccta tgacgcggtc gtgctcgatc tcggcttgcc
     5161 gaagaaggac ggcttgagcg tgctcgagca gtggcgccgt gacgaccgca agatgctggt
     5221 gctcattctc accgcgcgcg agcgctggag cgacaaggtc gcagggatgg atgccggcgc
     5281 cgacgattat gtcgccaagc ccttccacat ggaggaagtg ctggcacggc tgcgcgcgct
     5341 gcttcgccgc gccgccggcc atgcccgcaa cgagatggaa tgcggaccgc tccgcctcga
     5401 taccaagacg gcgcgggtga ccgtggatgg ccgtgcggtg aagctcacat cgcacgagta
     5461 tcgggtgctc gcctatctca tgctgcacaa ggcgcgcgtc gtctcacgca ccgaactcgt
     5521 cgagcatctc tacgagcagg atttcgaccg cgactcaaat acggtcgaag tgttcatcgg
     5581 acgcctgcgc aagaagcttc ccggcgatct catcaagacg gtccgcgggc tcggctatcg
     5641 cctctcggac gcgggcgatg aggcataact ctctcgcgtt ccggctgttc gcctccgcgg
     5701 cggcgtggac gctcgtggtc cttcccgtca ccgccattct gctcgtttcg ctctaccgcg
     5761 aggccctcga gcgcaacttc gatacgcgac tcaacgtcta tctgaccagc ctcgtcgcct
     5821 cgagcacgca agagggcgcg gcgacgccga aagagccggc cgatttcggc cagccgatct
     5881 tcggcattcc cttctcgggc tggtattggc agatcaagcc gctcaataga gctgcgcgcc
     5941 cggacttcgt ctccgattcc ctgctcgacc agcaactcaa gctgccgagc cagagcggcg
     6001 ccgcgccgga cgcgagcctg acccgccgcg cctatatcga agggcccaac gatcagcgct
     6061 tgcgcatcgt cgagcgcgag atcaggcaga gagccccggg ctcggccccc tattcctatg
     6121 cggtcgccgg ggattcctcg gagatcgagc gcgatctctc agaattcacc atgatgctga
     6181 ttgcggcgct ggccgtggcg gggctcgggc ttgtggcggc gaccttcttc caggtgcggt
     6241 tcggactgtc gccgctccgc gccatccgcc aggggctcac cgccatccgt tcgggcgaag
     6301 cggagaagct cgaaggcgag ctgccgcttg agatcaagcc gctgcaacag gagttgaacg
     6361 cgctgatcca ctccaatcgc gagatcgtcg agcgcgcacg cacccatgtc ggcaatctgg
     6421 cgcatgcgct caagaccccg ctcagcgtca tcagcaacga ggcgcggacc caggaggggg
     6481 tgttggccgc gaaggtgatc gagcaggcgg agatcatgcg cgaccaaatc acccatcacc
     6541 tcgaccgcgc gcgcgtggcg gcgcgctcaa gcgtgatcgg cgacgtgact gatgtcggcg
     6601 ttgtgctcga ggcgctgaag cggacgctcg accgcatcta cgaagagcgg ggccttgagc
     6661 tcgagcttgc cgccacgccg ggactgaagt tccagggcga gaggcaggat ttcgcagaga
     6721 tggtcgggaa cctgctcgac aatgcctgca aatgggcgcg gtccaaggtc agggccagcg
     6781 ccgagaggaa tggcgccgac gcgcggttca ccgtcatcgt cgatgacgac ggaccaggtc
     6841 tgaccggggc cgaacgggca agcgcggtga agcggggcca gaggctcgac gagacgaagc
     6901 cgggctcggg gcttgggctc tcgatcgtcg ccgatctcgc ccatctctat aagggacggt
     6961 tcgaactcga accctcgccg caagggggct tgcgcgcccg cctggagctg cccgcggcct
     7021 agctgttagc gtgctctagg aaacccgaag atgcgtaagc cgggcgggac cggagaggac
     7081 cgcttaatgt aaatgtgcat ggtaagcagc gcgccgaagg aggcgagtag cacaaataga
     7141 gcccccatta gaatggccat cttcttggag gccctggccc catccgtttc gtggataaac
     7201 attcccccgg gcccaacccc cctcgtgccc gcaaccctac cacctttggg ggcgctgcgc
     7261 ttcgggcgct aaactcatgg aagcgtctgg actcacgcgt gtttctcagg accctcaacc
     7321 caaatttggc cagaattgtt atttgttaag aatggcgtag cacgattaga ctgacctcag
     7381 tctcttgccg ggggccggcc gtggcgttag aggccgaagg gggacgcgat gagacgtgca
     7441 ccgatccttt ccattcttgt catttcgacc gtgcttgcgg cttgcgggcc cggcaacaag
     7501 cagggcaccg ggaccatcgt cggggcggtc gccggcggcg cggcgggcgg cgcgatcgcc
     7561 ggctcgaaca accgtgcggg cggcgtggcg atcggcgccg tggttggcgg cattctcggc
     7621 aaccagatcg gccggagcct tgacgaggaa gaccgcaggc tcgccgcggc ggccgagtat
     7681 caggcgctcg aatatggaca ggcgggagcc tcgaccccct gggacaatcc ccagacgcag
     7741 catcacgggc agatcgtgcc gggcaagccc tatcaacagg ggacccaata ttgccggccc
     7801 tacacccaca ccatctatat cggcggccag ccgcagacgg cgcgcggcac ggcctgccgc
     7861 cagcccgacg gcacctggca gacggtgggc taggccccgc gcctgcttgc gttaagggtc
     7921 gcagtctccc cgctatttcg cggaagtcat gtctttggcg tcccggtgct tgctccctct
     7981 cgcgcctttc cctagcctga tcctctgttt ttctgccgga ggctcgcgct cccgtgtttg
     8041 tctggctcgt cctcgccggc cttacggcga tcgttctttt cgtcctgttg cgtccgctca
     8101 tggcagccgg gatggaaagt cgcgcggcag aatccttcga cgccgccgtc tatcgcgatc
     8161 aactgaagga gatcgagagc gatcgcgcgc gcggcctgat cggcgatcgg gaagccgaag
     8221 ccgcacgcat cgagattgcg cggcgcctcc ttgccgcgga cgaaaaag
//
LOCUS       DASUQP010000047         9939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1929338, whole genome shotgun sequence.
ACCESSION   DASUQP010000047 DASUQP010000000
VERSION     DASUQP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929338"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1070
                     /locus_tag="VFY74_02160"
     CDS             384..1070
                     /locus_tag="VFY74_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930544.1"
                     /translation="MASAWTAATAREAALILRQRLSVARKWSAVHGGILARNSLAGAR
                     IASAWTASTLREAGLILRQRLSVARKWSTVHGRILARNSLAGARIASAWTASTLREAG
                     LGLALALKRTAVTVRLFARAARVEARRASSWGAAHSRTAAVVLKRNMADGAAWSSAKA
                     KTTAQASIAASRIGYSWAALRMRQASNGFFRATRPVADQNHRALVVRRSTAIICFEPK
                     RGGHPALRAG"
     gene            complement(1087..1251)
                     /locus_tag="VFY74_02165"
     CDS             complement(1087..1251)
                     /locus_tag="VFY74_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930545.1"
                     /translation="MTTPAVFVGAHGDRAELEAAQKAAECERQGQKEQAVDWRRIQAA
                     IKEMRGPHAS"
     gene            1468..2514
                     /locus_tag="VFY74_02170"
     CDS             1468..2514
                     /locus_tag="VFY74_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5930546.1"
                     /translation="MRTGSSALGLLAGAIALGLAGLPLEARAGAPESCAPPPVSDLVV
                     NVKDKGAVGDGRADDSAAIQAAIDAVAGTKGTVLVPDGVYLVETVGKTRVKLGSDMTL
                     KLAQGAVLKAIPNDEPKYAVLTISGVANVWVTGGTLEGDRNEHRGKSGEWGMGIRIDD
                     GAKHITISGLTSKKMWGDGFYVQEAEDVRFCGVTADGNRRQGLSIIAANGLLVLNSTF
                     KNTHGTRPSAGIDLEPDKERQTISHVRIEGSKFLDNAGEGILVAGKKARISKVEMMRN
                     TFRGNRPFVVENAPGIAASICGNRQTSKQPAASGSFNTYAEPVEIVALQNDCGEMGFV
                     IDRGNTKKKKKRAN"
     gene            complement(2519..2836)
                     /locus_tag="VFY74_02175"
     CDS             complement(2519..2836)
                     /locus_tag="VFY74_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5930547.1"
                     /translation="MRNLIINAATHGVRARVEVAHTNGTARIVIEDDGPGLPPDLIGR
                     VFEPFFRADPARRQGIRGAGLGLTIAREIVQRAGGTIAISNGANGGLRQIVELPKAEE
                     ALN"
     gene            complement(2739..3713)
                     /locus_tag="VFY74_02180"
     CDS             complement(2739..3713)
                     /locus_tag="VFY74_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5930548.1"
                     /translation="MTLLTLVLFYLLGPPKRVHSLAPVAQQIETLVRVADEGSAAVSL
                     VPQPAPGRVYERLTQRLRDTLAARGSDLAVMVSRDGWRAPLTVSIPIKSKGWVLFPIT
                     DFPPPGAPWRNLVRWLLLITFGAIVIAVFVANRMVRPLALLESAVASVGPDGALPTLP
                     ERGPAEVRATAKALNSLSSRLKRAMESRMRLVAAAGHDLRTPITRMRLRAEFVADAEE
                     RERWLNDLDELERIADSAILLVREEWVKAPPELIELDQLVGSIGEELRGPESRCDRDG
                     RRTREGAREQARAQSRAAQPDHQRGDAWRQSAGRGRPYERNCAHRHRG"
     gene            complement(3767..4494)
                     /locus_tag="VFY74_02185"
                     /pseudo
     CDS             complement(3767..4494)
                     /locus_tag="VFY74_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            4798..5289
                     /locus_tag="VFY74_02190"
     CDS             4798..5289
                     /locus_tag="VFY74_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX5930549.1"
                     /translation="MHGPRHHGKWARCDAGPHGHGFRHGRHGSGHLAQRLSVMETEIG
                     IRADQIDAWRDFTDAALAAMKRPSFPDATDKAPFALARSMADQTIARAKSAEALLKAI
                     DALKAKLTPEQLNKVADIEARFGAHFDRGRGSHSPRSPDGDDDPGGDEPNERDETPPS
                     SEP"
     gene            complement(5365..6189)
                     /locus_tag="VFY74_02195"
     CDS             complement(5365..6189)
                     /locus_tag="VFY74_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5930550.1"
                     /translation="MGWRLRDSERIVSGNEGLLGEKHFLIALTRVRNEALLLPDTLDY
                     LARHVDAIVAYDDASSDDTVEILRRHPKVALIVANEAWEQDVAARKLAEGRHRGLLLD
                     MARAELPHDWMLCFDPDERVTGDLRAFLRGLGEGECDAVRIRLFDAYMTEDDHAPYTR
                     GQELLDFRRFYGPEQRNILMLWRNRPEIVFAEGDGRTPRGMTKEKIDLYCQHYGKSLS
                     VAHWEETCDYYIRHFPFETYGKKWTERKGQAIHAESDFKRPLYEWGEALFANAVPM"
     gene            complement(6192..7379)
                     /locus_tag="VFY74_02200"
     CDS             complement(6192..7379)
                     /locus_tag="VFY74_02200"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5930551.1"
                     /translation="MSDLAATLSDERLRLCLALRDWLVGEARDIGDPNVIIEGVCLKL
                     RDAGMPIDRAVSAVEFRHAERAANARIWEFGRPAREHIYGHDRGSEATGPRPLAEAHR
                     LNDWIISWLPDLPDETYDIVPELKAAGYTHHIAVPVALPNGMLNGFTFATRAPNGFSA
                     EDIATLRAIVPTLSALQEILALRRVMREVMRMYVGDEPHLRILSGDVRRGEVLRIRAA
                     ILFADMRDFTGLSAHLSEEEATALVNDYYDCIVPPIDERGGEVLKFLGDGILAIFRPG
                     EDTAGGACLRAFAAARAGLLRVESHNAGSNIRFGVGIALHFGHAAFGNVGSGARLDYT
                     VIGRDVNLAARLADLCGELDECLLLSAAFQSRLSEQELRRLGAFPLKGVEEAQTVFAP
                     VGP"
     gene            complement(7456..8202)
                     /locus_tag="VFY74_02205"
     CDS             complement(7456..8202)
                     /locus_tag="VFY74_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5930552.1"
                     /translation="MAGHSKFKNIQFRKGAQDKKRSKLFSKLAREITAAARMGLPDPG
                     MNPRLRTAVQAGRAQNMPKDNIERAIKKSQEAGGANYEEVRYEGFGAGGVGVIVEALT
                     DNRNRTASEVRSIFTKFGGNLGETGAVSFNFDRLGAIEFDADKASPDAMLEAAIEAGA
                     EDCESAADGHVVYCHPDQLHQIAKALEAKFGEPRAARILWRAKTSVPIGEEAGQRLLR
                     MIEALEDSDDVQDVYANFEIADEVLERLSA"
     gene            8395..8649
                     /locus_tag="VFY74_02210"
     CDS             8395..8649
                     /locus_tag="VFY74_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930553.1"
                     /translation="MKPFLKPHAAFSEDKAMPPTTTLSALCSEAADHVAGARLSLSDD
                     GTDADRALVHLDEAIHCLRRLLAHGRAVAAKDARARPRPL"
     gene            complement(8656..9477)
                     /locus_tag="VFY74_02215"
     CDS             complement(8656..9477)
                     /locus_tag="VFY74_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX5930554.1"
                     /translation="MRILFLGDVVGRSGRAAVIEQLPELRERYRLDFVVVNGENAAGG
                     FGITEQILVELLDAGADVVTMGNHVFDQRETLVFIERYDRLLRPINFPAGTPGKGAGL
                     FKASAGADVLVINAQGRVFMADLDDPFRAVEHELEACGLKTRADAILIDFHAEATSEK
                     EAMGHFADGRATAVIGTHTHVPTADERVLPKGTAYISDAGMCGDFDSVLGMDKQEPLQ
                     RFLTKIPSGRFAPALGEATLCGVAIDVDDRTGLARAIAPLRLGGKLSRAEPAFWS"
BASE COUNT         1777 a         3249 c         3183 g         1730 t
ORIGIN      
        1 ttgcggcaag gacggtcgtc cttgcctatg tcagcgtgcg cgacgccgtc actggctgca
       61 tcgactgggt tcgcccgcgc gcatacgcgc tcgcgctctt gttgcggcga tggcttgtcg
      121 cttcttggac ttggctcgca gcgacgtccc gggcgtccgc cgcgctgttc caacgatggt
      181 gttctgacgc atgggtttgg acgcgcggaa atgcggcgat ccttgcgcgc gtctcgcgaa
      241 aaggcgttgc gatcgcgtca tcttggatcg caacgacatc ggtggcactg gccgcggcac
      301 ttctgcattg gctggtgaga acttggagac gaacgagagc ccttgcccgc gtcctttggc
      361 ggaagtccca ttacggagca ggcgtggctt cagcgtggac cgcagccacg gcgcgcgaag
      421 ccgccttgat ccttcgccaa cggctgtcgg tggcccggaa atggagcgcc gttcacggcg
      481 gcattctcgc gcgaaattcc ctcgccggcg cccgtatcgc ttcggcctgg accgcgtcca
      541 ccttgcgcga ggccggcttg atcctgcgcc aacggctgtc ggtggcccgg aaatggagca
      601 ccgttcacgg ccgcattctc gcgcgaaact ccctcgccgg tgcccgcatc gcttcggcct
      661 ggaccgcgtc caccttgcgc gaggcaggtt tggggctcgc tctcgccctg aaacggactg
      721 ccgtcactgt tcggctgttc gcgcgcgcgg cgcgtgttga agcacggcgc gcatcgtcat
      781 ggggcgcggc tcactcgcgc acggccgccg tcgtactgaa acgcaacatg gccgacgggg
      841 cggcgtggtc gtccgcgaaa gccaagacca cggcgcaagc ctcgatcgcc gcgagccgga
      901 taggttattc ctgggcggcg cttcggatgc ggcaggcctc gaacggtttc ttccgtgcaa
      961 cccggccggt tgccgaccag aaccaccgcg cgctcgttgt caggcgaagc acggcgatca
     1021 tttgcttcga gccgaagcgg ggaggccatc ccgctcttcg cgcgggctag accccgcgcc
     1081 gtccgctcag ctcgcatgag gaccgcgcat ctccttgatc gcggcctgaa tccggcgcca
     1141 gtcgaccgct tgttcctttt ggccctgccg ctcgcattcg gcggccttct gggcggcctc
     1201 aagctctgcc ctgtcgccat gggcgccgac aaaaacggcg ggcgtagtca tggatctgct
     1261 tggcttccat ggaccttcct cctgtttttc cgaagacctc gctccagggg caatacgccg
     1321 ccccgtcctt gcgctgggtc aagctaagtt cgatgcgaga tttcgatgct gcgaggcggg
     1381 gcgccgaggg ttgccgctcg cgccgccaaa tcttcgcaat gttcctgata atgattcacg
     1441 tttgggccga tacgaaggca actcgctttg agaacgggat caagcgctct cggactgctc
     1501 gccggcgcaa tcgctctcgg cctggcgggg ttgccgctgg aggcgcgtgc cggcgcgccg
     1561 gagagttgcg cgcccccgcc ggtctccgac cttgtcgtca acgtcaagga caagggtgcc
     1621 gtgggcgacg gccgcgccga cgactccgcg gctattcagg ctgcaatcga cgcggtggcc
     1681 ggcaccaaag gcacggtgct cgtccccgat ggcgtctacc ttgtggagac cgtcggcaag
     1741 acacgggtca aactcggaag cgacatgacg ctaaagctcg cccaaggtgc ggtcctcaag
     1801 gcgatcccca atgacgagcc gaaatacgcc gttctcacca tctccggcgt cgccaatgtg
     1861 tgggtcaccg gcggcacgct cgaaggcgac cgcaacgagc accgcggcaa gtccggcgaa
     1921 tggggcatgg gcatccgcat cgatgacggt gcgaagcaca tcaccatcag cggattgacc
     1981 tcgaaaaaga tgtggggcga cggattctac gtgcaggaag ccgaggatgt tcgcttttgc
     2041 ggcgtgacgg ccgacggaaa ccgccgccag ggcctgtcga tcattgccgc gaacgggctg
     2101 cttgtcctca actccacatt caagaacacg cacgggacgc gcccgagcgc gggcattgat
     2161 ttggagcccg acaaggagcg gcagacgatc tcgcacgtcc gtatcgaggg ttccaagttc
     2221 ctcgacaatg cgggcgaggg cattctggtc gctggcaaga aggcgcgcat ttccaaggta
     2281 gagatgatgc ggaacacgtt cagaggcaat cggcccttcg tggtcgagaa tgcgccgggc
     2341 atcgctgcgt cgatctgcgg caaccggcag acgagcaagc agcccgcggc ctccggcagc
     2401 ttcaatacct atgccgaacc ggtcgagatc gttgcgcttc aaaacgattg cggcgaaatg
     2461 ggcttcgtca tcgaccgcgg caacaccaag aagaaaaaga agcgcgccaa ctgaaccgtc
     2521 agttgagcgc ctcctcggct ttcggcaatt cgacgatttg tcttaggccg ccattggcgc
     2581 cattggagat ggcgatcgtt ccgccggcgc gctgcacgat ttcccgcgcg atcgtgagtc
     2641 caagtcccgc gccgcgaata ccctgccggc gagcgggatc ggcgcggaag aacggctcga
     2701 acacgcggcc aataagatcg ggtgggagcc ccggcccgtc atcctcgatg acgatgcgcg
     2761 cagttccgtt cgtatgggcg acctcgaccc gcgctctgac gccatgcgtc gccgcgttga
     2821 tgatcaggtt gcgcagcgcg cgattgagcg cgagcctgtt cgcgcgcacc ttcacgggtg
     2881 cgacggccgt ctcggtcaca tcgagattct ggtcccctga gttcctcgcc gatcgagccg
     2941 accaattggt caagctcaat cagctcgggc ggtgctttga cccattcctc gcgcaccaac
     3001 aggatggcgc tgtcggcaat ccgctccagc tcgtcgagat cgttgagcca gcgctcgcgc
     3061 tcctccgcat ccgcgacgaa ctcggccctg agccgcatgc gggtgatcgg cgtgcggaga
     3121 tcgtgaccgg ccgccgccac gagccgcatg cggctttcca tggcgcgctt gagccgcgac
     3181 gagagcgagt tcaacgcctt ggccgtcgcc ctgacctcgg ccggaccccg ttcaggcagt
     3241 gtcggcaagg ctccgtccgg tccgaccgac gcgaccgcac tctcaagaag cgcaagcgga
     3301 cgaaccatgc ggttggccac gaaaaccgca atgacaatgg cgccgaaggt gatcagaaga
     3361 agccatctga cgaggttgcg ccacggcgcc cccggcggcg ggaaatcggt gatcggaaac
     3421 agcacccagc ctttgctctt gatcggtatc gaaacggtga gcggtgcgcg ccagccgtcg
     3481 cgcgagacca tcacggcaag atcggagccg cgcgccgcca gcgtgtcgcg aagtctctga
     3541 gtcaggcgct cgtagacgcg acccggagcc ggctgcggaa cgagtgaaac cgcggcgctg
     3601 ccttcatcgg cgacacgcac gagcgtctcg atctgctggg ccacgggggc gagcgaatgc
     3661 acgcgcttgg gcgggcccag caggtagaac aggacgaggg tcaagagggt cacgaccgag
     3721 acgatcgcca ccgccaggag cacggcaatc ttgacacgaa gcgtgttcat gacgcggcgt
     3781 cctgaatttc gaccttggcg gcgagctgat agccccgttg cgcaccgtct tgaatagccg
     3841 cgatgcgccg ccgcccgcga gcttgcggcg aaggcggctc acgagaacgt cgatcgagcg
     3901 gtcgagcgga tcggcctcgc ggccgcgcgt gagatcgaga agctgatcgc gcgacaacac
     3961 ccggccgggc cgttcgagga aggtcttgag cagatcgaac tccgcgccgg tcaggacgac
     4021 gtcgccgcct cggggatcag tcacgcgccg cgtctgcgga tcagcggtga acccttcgaa
     4081 gcgatagatc ttgccctcgc tcacttgcgg cagcgtttcg gcaccgcgac ggagcaccgc
     4141 ccgcacccgc gcgatcagct cgcgcggatt gaacggtttg ccaagatagt cgtcggcccc
     4201 gatctcaagg ccgacgatgc ggtcgacatc ctccttcagc gcggtcagca gaataatggg
     4261 tatccgcgag cgctcggcgc gcaggtcacg gcacacttcg agtcccgaac cgtcgggcaa
     4321 catcacgtcg agcacgatga gatcgaattg aacggtggcg agccgttcgt gcagctcggc
     4381 gcaattcgcc gccagcgcca cgcgaaagcc ctggctctcg atatagcggg ccaagagctt
     4441 gcgaatttcc gggtcgtcgt cggcgacgag aatgcgcggg ggtgcttgcg tcatcggagc
     4501 gaactcatcg ccagtttcta acgcaagcag cgggcgaacg agcggatttt tgtaacgacc
     4561 cagaggtccg gcctttcgcg caacgtttcg atacgaaata cccgccgacg gcaatgttcg
     4621 gacacaccaa gacctaccgg cgcaattttc accctctaga gaaggaggat caggaacgaa
     4681 caaggacgtt cgagcatgaa aactcttccc agatttttac ccttcctttt ggttttgctc
     4741 ctcggtctcg ccggtcttgc catcatgacc tcggcatggg gttcaccctc cggcggaatg
     4801 catgggccgc ggcatcatgg caagtgggcg cgatgcgacg cgggcccgca cggtcacggc
     4861 ttcaggcacg gccggcacgg gtcgggccac ctcgcgcaac ggctgagtgt catggagacg
     4921 gaaatcggca ttcgggccga ccaaatcgat gcttggcgcg atttcaccga cgccgcactc
     4981 gcggcgatga agcggccttc atttccggat gcaacggaca aggcgccctt cgcgcttgcc
     5041 cgaagcatgg ccgaccagac aatcgcgcgc gcgaaaagtg cggaggcgct gttgaaggcg
     5101 atcgacgctc tcaaagcgaa gctcacgccg gagcagctca acaaggtcgc cgatatcgag
     5161 gcgaggttcg gtgcccattt tgatcgcggt cgaggatcgc actccccacg ctcccccgac
     5221 ggggacgacg atccaggcgg cgatgagccg aacgagcgcg acgaaactcc cccatcgtcg
     5281 gagccgtgag gcgatggagg cggcggccta gccgccgcct ctcattcctt cattgtttcg
     5341 ttacagggcg cttcggcaat ccgctcacat cggaactgcg ttggcgaaca gcgcctcgcc
     5401 ccattcatag aggggccgct tgaagtccga ctccgcgtgg atcgcctgcc ctttgcgctc
     5461 ggtccatttc ttgccgtagg tctcgaacgg gaagtggcgg atgtagtagt cgcacgtctc
     5521 ctcccaatgg gcgactgaca gcgacttgcc gtaatgctgg cagtagagat cgattttctc
     5581 tttggtcatg ccgcgcggcg tgcggccgtc gccctcggcg aagacgatct cgggtcggtt
     5641 gcgccacagc atgaggatgt tccgctgctc gggaccgtag aaccggcgaa aatcgaggag
     5701 ctcttgcccg cgcgtatagg gcgcatgatc gtcttcggtc atgtaggcgt cgaacagcct
     5761 gatcctcacg gcatcgcatt cgccttcgcc gagcccgcgc agaaacgcgc gcaaatcccc
     5821 ggtgacccgc tcgtcgggat cgaagcagag catccagtca tgcggcagct cggcgcgcgc
     5881 catgtcgagc aagagacccc tgtgccggcc ttcggcaagc ttgcgggccg caacgtcctg
     5941 ctcccacgcc tcgttcgcca cgatcaacgc gaccttggga tgccggcgca ggatttcgac
     6001 ggtgtcgtca ctgctcgcat cgtcgtaggc gacaatcgca tcgacgtggc gcgcgaggta
     6061 gtcgagcgta tcgggcagga gcagcgcctc gttgcggacg cgcgtgagag cgatcaaaaa
     6121 atgcttttcg ccgagcaacc cctcgttgcc gctcacgatc cgctcgctgt cgcgcagccg
     6181 ccagcccatg ctcaaggtcc aacaggcgca aacacggtct gcgcctcctc gactcccttg
     6241 agcgggaacg cgcccagcct gcgcagctcc tgctccgaca ggcggctttg aaacgcagcg
     6301 gacagcaaca ggcactcgtc caattcgccg cacagatcgg cgaggcgcgc ggcgagattc
     6361 acgtcgcggc cgatcaccgt atagtccaga cgcgcgccgg agccgacatt gccgaaggcc
     6421 gcatggccga aatggagggc gattccaacg ccgaaacgaa tattgctccc cgcgttatgg
     6481 gactcgactc gcaagagccc ggcgcgtgcg gccgcgaagg cgcggagaca tgccccgcca
     6541 gcggtgtctt cgccgggacg gaagatggca agaatcccgt cgcccaggaa cttcagcacc
     6601 tcgccgccgc gctcgtcgat ggggggcacg atgcagtcgt aataatcgtt gaccagcgcg
     6661 gtcgcctcct cttccgacaa atgcgccgac aggccggtga agtcgcgcat gtcggcgaac
     6721 aggatcgcgg cgcgaatgcg caagacttcg ccgcgcctca cgtcgcccga gagaatgcgc
     6781 aagtgcggct cgtcgcccac atacatgcgc atgacctcgc gcatcacgcg ccggagcgcg
     6841 agaatctcct gaagcgccga aagcgtcggc acgatcgcgc gcaaggtcgc gatatcctcg
     6901 gcggagaagc cgtttggcgc gcgcgtggcg aaggtgaagc cgttaagcat gccgttcggc
     6961 aacgcgacgg gaacggcgat gtggtgcgta tagcctgcgg ctttgagctc cgggacgatg
     7021 tcgtaggtct cgtcgggcaa gtcggggagc caagagatga tccaatcgtt gagccgatgc
     7081 gcctcggcga gcggacgcgg ccccgtcgct tcagagccgc gatcgtgccc atagatgtgc
     7141 tcgcgcgccg ggcgcccgaa ttcccagatg cgcgcattgg ccgcccgttc tgcgtgtcgg
     7201 aactcaaccg cgcttacggc ccggtcgatc ggcatgccgg catcgcggag tttcaggcaa
     7261 accccttcga taatgacatt ggggtcgccg atgtcgcgcg cttcgccgac cagccagtct
     7321 cgcagcgcga gacaaaggcg gagccgctca tcggaaagcg ttgcggcaag atcggacacg
     7381 agcctatccc ttgcggccta aacatcgcgc cgggcaacga tcggagccaa gcgccatcgc
     7441 cgaagcggcc ttctcttagg cgctgaggcg ctcgagcacc tcgtcggcga tctcgaaatt
     7501 ggcatagacg tcctgcacgt cgtcggaatc ttcgagcgcc tcgatcatgc gcaagaggcg
     7561 ctggcctgct tcctcgccga tcggcacgga ggtctttgcc cgccacagga tgcgtgcggc
     7621 ccgcggctcg ccgaacttcg cttcgagcgc cttggcgatc tgatgcagct gatcgggatg
     7681 gcaatagaca acgtgaccgt cggcggccga ctcgcaatct tcggctcccg cctcgatcgc
     7741 cgcttcgagc atcgcatcgg ggctcgcctt gtccgcatcg aactcgatcg cgccgagcct
     7801 gtcgaaattg aaggacacgg cgcccgtctc gccgagattg ccgccgaact tggtgaagat
     7861 cgagcgcacc tcgcttgcgg tgcggttgcg attgtcggtg agcgcctcga cgatgacgcc
     7921 gacgccgccc gcgccgaagc cctcgtagcg cacttcctcg taattggcgc cgcccgcctc
     7981 ctggcttttc ttgatggcgc gttcgatatt gtccttgggc atgttctggg cgcgccccgc
     8041 ctgcaccgcg gtcctgagcc gcgggttcat gccgggatcg ggcaagccca tcctcgccgc
     8101 cgcggtgatc tcgcgtgcga gcttggaaaa gagcttggag cgcttcttgt cctgggcccc
     8161 tttacggaac tggatgttct tgaatttcga atggccggcc atcgcccgat ccctattggt
     8221 tactgccgct gccggtcaag gggcttcgtc ccgccctcaa gcgcttgcgc atcatggtga
     8281 gaacgggccg tccgtcgccc aggcttagcg aagccttaac cctaacggcc cgacaaatga
     8341 aggcttggcg actcgcgcgg gccgcgcggg cttgcctgaa caaggggggc aatcatgaaa
     8401 ccgtttctca agccgcacgc cgccttcagc gaggacaagg ccatgccacc aacgacgaca
     8461 ctctcggcgc tctgctccga agccgccgac cacgtagccg gggcccgatt gtcgctgtcg
     8521 gacgatggca ccgacgcgga tcgcgcgctt gttcacctcg atgaggcgat ccactgcttg
     8581 cggcgcctgc ttgctcatgg ccgcgctgtc gccgccaaag atgcgcgtgc gcgacctcgc
     8641 ccgctctaac tttcctcaag accagaacgc gggttcggcc cgcgacagct tgccgccgag
     8701 ccggagcggc gcgatggcgc gcgccaagcc ggtccgatcg tccacatcga tggcgacccc
     8761 gcaaagcgtg gcctcgccga gcgcgggtgc aaaccgcccg gatgggatct tggtcaggaa
     8821 gcgctgcaac ggctcctgct tgtccatccc gagaaccgaa tcgaaatcgc cgcacatgcc
     8881 ggcgtcgctg atataggcgg tgcccttggg caggacccgc tcgtcggccg tcggcacatg
     8941 ggtgtgcgtg ccgatcaccg cggtcgcgcg cccgtcggcg aagtgaccca tcgcctcctt
     9001 ctccgaggtc gcctcggcgt ggaagtcgat gaggatggcg tccgcgcggg tcttgagccc
     9061 gcacgcttcg agctcatgct ccacggcgcg gaagggatcg tcgagatcgg ccatgaacac
     9121 gcgcccttgc gcgttgatca cgagcacatc ggcaccagcg cttgccttga acaaaccggc
     9181 gcccttgccg ggcgtccccg ctggaaaatt gatcggccgc agcaaacgat cgtagcgctc
     9241 gatgaacacc agcgtctcgc gctgatcgaa cacatgattg cccatggtca cgacgtcagc
     9301 gcctgcgtcg agcagctcta cgaggatttg ctcggtgatg ccgaagccgc cggccgcgtt
     9361 ctcgccgttc accaccacga aatcgaggcg atagcgttcg cgcaattccg gcaattgctc
     9421 gatgacggcg gcgcggcccg agcggccgac cacatctccc aggaacagaa tacgcatcgt
     9481 gcggccgatc cgctgttggc gagaaaagtc tcgccggcgt caatagcgaa tcgcggcgtg
     9541 tcccgccagc gtcgtcgcgg cgtgatcttc acaaggttga gggaagttgg aatggccagg
     9601 ccgcgatggc cgtcggagcg aagatcccgg gtgtccctac atcagtaggt gggcgccaaa
     9661 tggcggagac cacggtcccc gtcagggtcg gctcccatag gatcttatag ggccctggga
     9721 ttgtgctctt tcgagggaga cttctcgaac aagatcctgt cgatgggcga ggcgagcggt
     9781 ctgcaagaca aagagcttca agatacgctc ctgcgccaac tgataagcga aggcgtcctc
     9841 cattatcccg tggcgcagaa aatccacgat gcatgcagtc atcgaacagg cggctcgtgg
     9901 cgacgaggta gagcagcttg gcattgctct gctcgccgg
//
LOCUS       DASUQP010000048        12109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_839793,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000048 DASUQP010000000
VERSION     DASUQP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839793"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..790
                     /gene="secB"
                     /locus_tag="VFY74_02220"
     CDS             230..790
                     /gene="secB"
                     /locus_tag="VFY74_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX5930555.1"
                     /translation="MAKGKATGEQPKKTNGDGPSAESAQAVPAAGESAQGKPAPQLTM
                     LALYVKDLSFESPSAPKSLQGPGQNPQLRVNVNVNAGPRGEETYEVALQLEVNASSDL
                     GVIYNVELLYGGLFRLRAVPENLVQPVLFVDCPTILFPFVRRVLADAIRDGGFPPLML
                     DPIDFAKLYAKNFTPAQGNLQTSAKN"
     gene            complement(801..1487)
                     /gene="dnaQ"
                     /locus_tag="VFY74_02225"
     CDS             complement(801..1487)
                     /gene="dnaQ"
                     /locus_tag="VFY74_02225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX5930556.1"
                     /translation="MREIVLDTETTGLDPFDGHRVVEIGCVELVNCIPTGRVWHCHLN
                     PERDMPYQAFEVHGLSIEFLRDKPRFAELADNMLAFLEGAMLVMHNATFDFAFLNAEL
                     DRSSRPLLRWDRVVDTLALARRKHPGAPCSLDALCKRYGLDLSQREKHSALLDSRLLA
                     AVYVELVGGHQARLDFAINGAQAIMLGTAAGTVAERPRPLAPRLTAAEIEAHRAFVAT
                     LGAEALWLRY"
     gene            complement(1489..2121)
                     /gene="coaE"
                     /locus_tag="VFY74_02230"
     CDS             complement(1489..2121)
                     /gene="coaE"
                     /locus_tag="VFY74_02230"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110141.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5930557.1"
                     /translation="MMLVVGLTGGIGMGKSAAAERFAEQGIPVFNADLCVHQLYEGAA
                     VRPIEAAFPGVTREGRVDRKLLSERIAGSPERLRLLEETVHPLVVAAELDFLRAQEQR
                     GARLAVLEIPLLFETGADARVDVTVVVSAPHDVQRARVLARPGMTADKLEHLLARQLP
                     DAERRARADFVVDSGTTVEDMRIEIDRLIESLKSREGRVMDRLRRKDERE"
     gene            complement(2118..2951)
                     /locus_tag="VFY74_02235"
     CDS             complement(2118..2951)
                     /locus_tag="VFY74_02235"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5930558.1"
                     /translation="MRRACVIGWPVEHSRSPLIHRYWLREYGIDGAYEKEAVEPKDLA
                     RFLGTLKNLGYVGANVTLPHKEAALRLAAVADEAARTIGAANTLWLDEAGRLNASNTD
                     AYGFMTNLNAEAPDWNESRRPVTVLGAGGAGRAILHGLLAEGVRRILLANRTTGRAEA
                     LAKAFGPAVTLVDWRERARALSGCGLLVNATSLGLTGKEPLDLDLASLPTDAVVADLV
                     YSPLETGLLAAARARGNRTVDGLGMLLHQAVPGFARWFGVRPEVTPELRAHVAASLGE
                     R"
     gene            complement(2958..3581)
                     /locus_tag="VFY74_02240"
     CDS             complement(2958..3581)
                     /locus_tag="VFY74_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5930559.1"
                     /translation="MSSFLQAGRPPLILASASKSRARILEAAGLAFIVEPPGLDEAVM
                     RQAISGEGALSPHDVAEVLARAKAEAVSDLAREAYVIGGDQVLVQGETIYAKPDSMKA
                     ARRQLLDLSGKKHTLHTAVAVATNGETVWAETMVATLTMRKLSPEFIGRYLAAAGEQV
                     LASVGAYQIESLGVQLFEAIEGDYFSILGLPLVPLLDTLRRVGAIEG"
     gene            complement(3578..4411)
                     /locus_tag="VFY74_02245"
     CDS             complement(3578..4411)
                     /locus_tag="VFY74_02245"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX5930560.1"
                     /translation="MDILRTFHLHMISDATGETLITIAKAVRVQYAKVRAIEHLHALV
                     RNRRELERAVRDVEASPGIVLYTLVNRELAEELERACKRLNVPCVPALKPILQVFESY
                     LGTPSTPTIGGQHVLDADYFRRIEALNFTMLHDDGHLPPNLNDADIVLIGISRTSKTP
                     TSIYLANRGFKTANVPLVPGMPLPLQLEIPTTAFVVGLVASPERIAQVRQHRVIEHAH
                     NHLEDYVDREAIAAEIAQTRALCARHGWPIIDVTRRSIEETAAAIIRLYHDREAPALE
                     A"
     gene            4841..5890
                     /gene="hemE"
                     /locus_tag="VFY74_02250"
     CDS             4841..5890
                     /gene="hemE"
                     /locus_tag="VFY74_02250"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5930561.1"
                     /translation="MLRVRPSENPFLSALAGETASPPPIWLMRQAGRYLPEYREVRGK
                     VRSFLDLCLNPKLAAEVTLQPSRRFSFDAAIVFSDILIVPYALGQKVEFIEGEGPRLE
                     PIRDSSAISRLDRGGTAKALAPVYETIERVVAELPRAVPLIGFCGAPWTVATYMVEGG
                     GSKDQAEARLLAYRDPATFQRLIDLLVDSSADYLADQVRAGARALQIFDSWAGSLPED
                     AFDRWCIAPTRRLIEQVRAKVPGTPVIGFPRGAGVLAERYAKETGIDAIGCDTSQPLG
                     WIKDNLQTLVPVQGNLDPVLLVAGGAELDRRAEAILRSLGPGPFIFNLGHGILPETPI
                     DHVARLVHLVKSGPA"
     gene            5911..6342
                     /gene="hemJ"
                     /locus_tag="VFY74_02255"
     CDS             5911..6342
                     /gene="hemJ"
                     /locus_tag="VFY74_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEX5930562.1"
                     /translation="MLLAYIKAFHVIAIIAWMAGLLYLPRLFVYHAASKPGSEQSETF
                     KVMERRLLRYITTPAMIASWVLGLWLAFSGVIDWSRDGWFHAKLILVLALSAYHGLLA
                     IWTKDFALDRNTRSVRFYRIANEIPTILMIFIVILAVVRPF"
     gene            6581..7846
                     /gene="rho"
                     /locus_tag="VFY74_02260"
     CDS             6581..7846
                     /gene="rho"
                     /locus_tag="VFY74_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5930563.1"
                     /translation="MQEMKLQDLKTKSPTELLSFAEEVAVENASAMRKQELMFAILKQ
                     LAARDVEITGTGVVEVLQDGFGFLRSPDSNYLAGPDDIYVSPSQIRRFGLRTGDTVEG
                     QIRSPKDGERYFALLKVNTINFEDPEKQRHKIHFDNLTPLYPDEWLQMEIEKPTTKDL
                     TGRVIDIVAPLGKGQRGLIVAQPRTGKTIILQNIAHSVTANHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVI
                     FEEFKGTGNSEIVLDRKVADKRIFPAIDIARSGTRKEELLVQGDQLKKMYVLRRILNP
                     MGIQDAIEFLLDKLKQTKGNSDFFDSMNT"
     gene            complement(8035..10095)
                     /locus_tag="VFY74_02265"
     CDS             complement(8035..10095)
                     /locus_tag="VFY74_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5930564.1"
                     /translation="MQERVDGNELARETSPYLLQHKDNPVHWRPWGDAALKEARATGK
                     PILLSVGYAACHWCHVMAHESFEDDATAAVMNDLFVNIKVDREERPDIDAIYMGALHA
                     LGEQGGWPLTMFLTSEAEPFWGGTYFPKDERFGRPPFVRVLNEVSRIYHAEKDKVRHN
                     AQILKERLAPRRGGVRAAPPPEAQLADLARRFVQAVDPVYGGISGAPKFPQPQFFEFL
                     WRAGLRYGLPHPREAVDLTLTRIAQGGIYDHLGGGFARYSVDARWLVPHFEKMLYDNA
                     ELVTMMTEVWRETKSPLYAERIAETIDWLMREMTVEGGAFASSLDADSEGEEGKFYVW
                     SLPAIEDALGAEDAKLFAEVYGVTSQGNFEGHNILNRLDAIELRDAETEARLAAMRQK
                     LLARRSSRVRPGFDDKVLADWNGLMIAALANAADAFDRVDWLDAAERAFDFVCTRMIS
                     NGRLFHAYRAGEAKAPATANDYANMIDAALALASVTGKSSYVERAREWTDVLDRHYWA
                     EDLGGYYFAADDTSDLIVRPFSGQDDATPNANAVMVSNLMALLLWTGEARYRDRAEAI
                     LSGFAGAMSESVLAHAGMLAATLDVYAPALVVLLVPEGRDARELRRALADVSLPNAVV
                     QEVREGEGGASALSPSSPAHGKTSIDGKPTAYVCIGPQCSLPVTAPAALVATVKAARQ
                     VGTV"
     gene            10408..11751
                     /gene="mnmE"
                     /locus_tag="VFY74_02270"
     CDS             10408..11751
                     /gene="mnmE"
                     /locus_tag="VFY74_02270"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX5930565.1"
                     /translation="MITADADTIVAPASGAGRAAIAVVRISGERTRDVLDALCGGAPP
                     PRRASLRAIGLANAPIDRGLVLWFPGPQSFTGEDMAELHVHGSRAVIRAVIDAVLSIP
                     GTRLAEPGEFARRAFEKGKLDLTEVEGLADLVNAETEAQRRQALAQAEGSLRALYEGW
                     RAELVRAQALIEAGLDFADEGDVIADVSAKADPIVARLLAAIAHHLLDRRGERLRDGF
                     KVVIAGAPNAGKSSLLNALAKRDAAIVSEEAGTTRDAIEVHLDLGGVPVMLIDTAGLR
                     AAEGKIEAEGIRRALARAGDADLVLWVVDATAPQWHAPLGIGLGKSGEADAAAVTVLN
                     KIDLAPGAGAGTDCLKLSAKTGEGIEHLVDALAEKTTLAAEQRAGAPLLTRTRHRVEL
                     EGARAALERYGDAALGPEFKAEELRIAARHLGRLTGLIDVEEVLGAIFAEFCIGK"
     gene            11828..>12109
                     /locus_tag="VFY74_02275"
     CDS             11828..>12109
                     /locus_tag="VFY74_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042440689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5930566.1"
                     /translation="MNKGFDVIVIGGGHAGCEAASASARMGAATALVTHSFSTIGEMS
                     CNPAIGGLGKGHLVREIDALDGLMGRAIDRGGIQFRMLNRSKGPAVRGPR"
BASE COUNT         2136 a         3953 c         3886 g         2134 t
ORIGIN      
        1 tgagatttgc cagcgggatc cttcagcgcg agcgaggagg atcccattcc tgcccgcgct
       61 ccccaagctt acggcattag caggctccaa gccaacccta aaccccgtcg ctcttaatac
      121 gctgttgcaa cagcggcccg atgaagcgcc atcgggcgaa tgccgttagc gccatgaatt
      181 gcggcccgct tcgatcccat gctagccagg acatggagga tcggagccga tggcgaaagg
      241 caaggcgacg ggcgaacagc ccaaaaagac gaatggcgat gggccgtcgg cggaatcggc
      301 gcaagcggtg cctgcggcag gggaaagcgc ccaaggcaaa cctgcgccgc agctgacgat
      361 gctcgccctc tacgtcaagg acctctcttt cgaaagtccg agcgccccga aatcgttgca
      421 gggccccggc cagaacccgc agctcagggt caacgtcaat gtgaatgccg gcccgcgcgg
      481 ggaggaaacc tacgaggtcg cgttgcagct cgaggtcaat gccagcagcg atctcggcgt
      541 catctacaat gtcgagctgt tgtatggcgg gctgttccgc ctgcgcgccg ttccggagaa
      601 tttggtgcag ccggtcttgt tcgtcgattg tccgacgatc ctgtttccct tcgtgcgccg
      661 cgtccttgcc gatgccatcc gcgacggggg cttcccccct ctgatgctcg atccgatcga
      721 tttcgccaag ctctacgcca agaacttcac cccggctcag ggaaatcttc aaacgagcgc
      781 caagaactaa atgagctccg ctagtagcgc agccacaggg cttcggcgcc gagggtcgcc
      841 acgaaagcgc ggtgcgcctc gatttccgcg gcagtcagtc gcggtgcaag cggccgtggc
      901 cgctcggcga cggtcccggc ggccgtcccc agcatgatcg cttgcgcgcc attgatagca
      961 aaatcgagcc gcgcctgatg cccgccgaca agctcgacat agacggcggc gagaagcctg
     1021 ctgtcgagga gcgcgctgtg cttctcgcgc tgtgacaggt cgagaccgta gcgcttgcac
     1081 agagcatcga ggctgcaagg cgcgccggga tgctttcttc gcgccagtgc cagcgtgtcg
     1141 acgacgcgat cccagcgcaa aagggggcgg gacgagcgat cgagctcggc gttgagaaag
     1201 gcgaaatcga aggtggcgtt atgcatcacg agcatggcgc cctcgaggaa ggcgagcatg
     1261 ttgtcggcga gttcggcaaa gcgcggcttg tcgcgcagga actcaatgga gaggccatgc
     1321 acttcgaagg cctggtaggg catgtcgcgc tccgggttca gatggcaatg ccacacccgg
     1381 cccgtcggga tgcagttgac aagctccacg cagccgatct cgacgactct gtggccgtcg
     1441 aacgggtcga gccccgtggt ctcggtgtcg aggacgatct cgcgcaagct attctcgctc
     1501 gtctttgcgg cgcagccggt ccatcacgcg cccttccctg cttttaagcg attcgatcag
     1561 tctatcgatc tcgattcgca tgtcctcgac ggtcgtcccg ctgtccacaa cgaagtcggc
     1621 gcgcgcccgc ctctcggcgt cgggaagctg gcgtgcgagc agatgctcga gcttgtcggc
     1681 ggtcatgccg gggcgggcga gcacgcgggc gcgttggacg tcgtgggggg cgctcaccac
     1741 cacggtcaca tcgacgcggg catcggcgcc cgtttcgaac aggaggggga tctcaagcac
     1801 agcgaggcgc gcgcctcttt gttcctgcgc gcggagaaag tcgagctcgg ccgcgaccac
     1861 caggggatga acggtctcct cgagcagacg caggcgctcg ggcgatcccg cgatccgctc
     1921 cgacaatagt ttccggtcga cgcgtccctc acgcgtaacg cccgggaaag cggcctcgat
     1981 cgggcggacc gctgcccctt catagagctg atggacgcaa agatcggcgt tgaacacagg
     2041 gatgccctgt tcggcgaagc gctcggccgc cgccgatttc cccatgccga tgccgcccgt
     2101 gagcccgacg accagcatca tcgctctccg agagaggcag cgacatgggc ccgcaattca
     2161 ggcgtcacct ctgggcggac gccgaaccag cgcgcaaaac cggggaccgc ctgatggagc
     2221 agcatgccga gcccgtcgac ggttcgattt cccctggcgc gcgcggcggc gagaagtccg
     2281 gtttcgagcg ggctatagac gagatcggcg acaacggcgt cggttggcaa tgaagcaaga
     2341 tcgaggtcga gaggttcctt gccagtcagg ccaaggctgg tcgcattgac gaggaggccg
     2401 caacccgaaa gcgcgcgtgc acgttcgcgc caatcgacca aggtcaccgc tggtccaaag
     2461 gctttcgcca aggcttcggc acggccggtc gtccggttgg cgaggaggat gcgtctcacc
     2521 ccctccgcaa gaaggccgtg caggatggcg cggccagcgc cgcccgcgcc gagcaccgtc
     2581 accggccgcc ggctctcgtt ccagtcgggc gcctcggcat tgagattggt catgaagcca
     2641 taggcatccg tgttgctcgc gttgagccgg cccgcctcgt cgagccacag cgtgttggcc
     2701 gcgccgatcg ttcgtgccgc ctcgtcggcg accgccgcga gccgtagcgc cgcctccttg
     2761 tgcggcaggg tgacattggc gcccacatag ccgaggttct tgagcgtgcc gaggaaccgg
     2821 gccagatcct tgggttccac cgcttccttc tcataggcgc cgtctatgcc gtactcccgg
     2881 agccagtaac gatggatcaa aggtgagcgg gagtgctcga ccggccagcc gatgacacag
     2941 gcgcgtcgca ttgtccgtca gccttcgatg gcgcccacgc gccgaagcgt gtcgaggagc
     3001 ggaacgaggg gcaggccgag gatcgagaaa tagtcgccct cgatggcctc gaacagctgc
     3061 acgccgagcg actcgatctg ataggcgccg accgaggcaa ggacctgttc gcctgccgcc
     3121 gcgagatagc ggccgatgaa ctccggcgac agctttcgca tggtgagtgt cgccaccatc
     3181 gtctccgccc agacggtttc gccattggtc gcaaccgcga ccgcggtatg aagcgtatgc
     3241 ttcttgccgc tgagatcgag caattggcgg cgcgccgcct tcatgctgtc gggcttcgcg
     3301 tagatggttt cgccctggac cagcacctga tcgccgccga tcacgtaagc ttcccgggca
     3361 agatcgctca ccgcctcggc cttggccctt gccagaacct cggcgacatc gtgggggctt
     3421 agcgccccct cgccgctgat ggcttggcgc atgacggcct cgtcgagacc cggcggctcg
     3481 acgatgaagg ctagtcccgc cgcttcgaga atgcgcgccc gcgacttgct cgccgaggcg
     3541 aggatcaggg gcggacgccc ggcctggagg aagctgctca tgcttcgaga gccggcgctt
     3601 cgcgatcgtg gtagagccgg atgatggcgg ccgccgtctc ctcgatggac cggcgcgtga
     3661 cgtcgatgat cggccagcca tggcgcgcgc aaagcgcccg cgtttgggca atctcggcgg
     3721 cgatagcctc ccggtccaca tagtcctcga gatggttatg ggcgtgctcg atcacgcggt
     3781 gctgccggac ttgcgcgatc cgctcgggac ttgcgaccag accaacgaca aaagcggtgg
     3841 tcgggatctc gagctgcagc ggcagcggca ttcccggaac gagcggcacg tttgcggtct
     3901 tgaagccgcg attggcgagg tagatcgagg tcggcgtctt cgaggtgcgg ctgatgccga
     3961 tcagcacgat gtcggcgtcg ttgaggttcg gcggcaagtg gccgtcgtca tggagcatgg
     4021 tgaagttcag cgcttcgatg cgccggaaat agtcggcgtc gagcacatgc tggcctccga
     4081 ttgtcggagt cgaaggcgtg ccgagatagg actcgaacac ctgcaggata ggcttgagcg
     4141 cgggcacgca agggacattc agtctcttgc aagcgcgttc gagttcctcg gcgagctcgc
     4201 gattgaccag ggtgtagagc acgatcccgg gcgaagcctc cacgtccctc accgcccgct
     4261 cgagctcccg gcggttccgc accaaagcat gcaggtgctc gatcgcccgc actttggcgt
     4321 attggaccct gaccgccttg gcgatggtga tcagcgtctc gccggtcgcg tcggagatca
     4381 tatggaggtg gaaagttcgc aggatatcca tggcaatctc cttggataac cgcgatgggt
     4441 taagggagca actccggcag aggttctgcc ctctcccccc acctccccga aactggggtc
     4501 aagttcttca cagattcgct cttgtcgaca aaatgacgct tgtttgagac cggccgacaa
     4561 taccctcacc catccccagt tccgccggcc ttctacccac agactgtcca acgatttgca
     4621 agacgcgaag caccgccttt aacgtcacgt aatatcggcc ggtgtgacca ataattgatg
     4681 agataaccac atccgcttca gcccggatgt cttgccgctc ctgccaccgt ctaaagttct
     4741 gtagagagct tggtgacaga acggaatgcc cgctatccac gccgtaactg aaacaataga
     4801 aactctttta gagactctct tataatgatt tagaacaccc atgttgcgcg tgcggccatc
     4861 ggaaaacccg ttcctttctg cgcttgccgg ggagaccgct tcgcccccgc cgatttggct
     4921 gatgcgccag gcaggccgct atctcccgga ataccgcgag gtgaggggca aggttcgctc
     4981 cttcctcgac ctttgcctta acccgaaact cgcggccgag gtgaccttgc agccgtcgcg
     5041 gcgcttttct ttcgacgcgg cgatcgtctt ctcggacatc ctcatcgttc cttacgcctt
     5101 gggccagaag gtcgagttca tcgagggcga gggacccagg ctcgagccga tccgagacag
     5161 cagcgccatt tcacgccttg atcgcggcgg cacggcaaaa gcgctcgcgc cggtctatga
     5221 gaccatcgaa cgtgtggttg cggagctgcc aagagccgtg ccgttgatcg gcttctgcgg
     5281 tgccccttgg acggttgcga cctatatggt cgaaggcgga ggcagcaagg accaggccga
     5341 ggcgcggctg ctcgcctatc gcgatccggc caccttccag cgcctgatcg atctcctcgt
     5401 cgacagctcc gccgactatc tcgccgatca agtgagggcc ggtgcgcgcg ccttgcagat
     5461 tttcgacagc tgggccggaa gtcttcccga agacgctttc gaccgctggt gcatcgcgcc
     5521 gacgcggcgg ctcatcgaac aagtcagggc caaggtgccg ggaacgcccg tgatcggttt
     5581 cccccgcggc gccggcgtgc ttgccgagcg ctacgccaag gagacgggta tcgatgcgat
     5641 aggctgcgac acgagccagc ccctaggctg gatcaaagac aatctgcaaa ccctcgttcc
     5701 ggtgcagggc aatctcgatc ctgtccttct cgtcgccggc ggcgccgagc tcgatcggcg
     5761 tgcggaagcg atcctcaggt cgctcggccc aggccccttc atcttcaatc tcggccatgg
     5821 cattctcccg gagacgccga tcgaccatgt cgcccggctc gttcatctgg tgaagtccgg
     5881 gcccgcctga tcgtctcctg gcaattgtcg atgctgcttg cctacatcaa ggccttccat
     5941 gtcatcgcga tcattgcgtg gatggcgggg ctgctctatc tgccgcggct cttcgtctac
     6001 catgcagcga gcaaaccggg ttccgagcag tccgagacct tcaaggtgat ggagcggcgc
     6061 cttctgcgct acatcacgac gccggccatg atcgcaagct gggtgctggg actctggctc
     6121 gccttttccg gggtgatcga ctggtcgcgg gacggctggt ttcacgccaa gctcatcctc
     6181 gtcttggcgc tgtcggccta tcacggcctg ctcgcgatct ggacaaagga ctttgccctg
     6241 gatcgcaaca cgcgatcggt gcggttttac cgcatcgcca acgagatccc gaccatcctc
     6301 atgattttca tcgtgattct cgccgttgtc cgcccgttct agaggctcgc ccaagcctat
     6361 tccgcccgcc gtcctggtct gctataagcg agtcgcatcc aattcgggac gctgtcgcgg
     6421 cccgctcttc ccccaaagat cgggtcaccc ggagtggagc aaggcgccga actttgcctt
     6481 cccgccgagg catttccgac cccctccccg taacgccgcg ctcccccgcg cggaactcga
     6541 aaacgcttaa gtcgtccatt tccccctgga gaagcagcgc atgcaagaaa tgaagctgca
     6601 agatttgaag accaaatcgc cgacggagct cttgagcttc gccgaggagg tcgccgtcga
     6661 gaacgcgagc gccatgcgca agcaggagct catgttcgcg attctgaagc agctggcagc
     6721 ccgcgacgtc gagatcaccg gcaccggcgt ggtcgaggta ttgcaggacg gcttcggctt
     6781 cctgcgctcg cccgactcga attatctcgc cggtcccgac gacatctacg tgtcgccgtc
     6841 gcagatccgg cgcttcggtc tccgcaccgg cgataccgtc gaaggccaga tcaggagccc
     6901 gaaggacggc gaacgctatt tcgccctgct caaggtcaac accatcaatt tcgaggaccc
     6961 cgaaaaacag cgccataaga tccatttcga caatctgacg ccgctctatc ccgacgagtg
     7021 gcttcaaatg gagatcgaga agccgaccac caaagacctc acggggcgcg tcatcgacat
     7081 cgttgctccc ctcggcaagg gccagcgcgg gttgatcgtg gcgcaaccgc gtaccggcaa
     7141 gacgatcatc ctccagaaca tcgcccactc ggtcacggcc aatcaccccg agtgctacct
     7201 catcgttctg ctcatcgacg agcgccccga ggaggtgacc gacatgcagc gctcggtgaa
     7261 gggcgaggtg atctcgtcca ccttcgacga gccggcctcg cggcacgtgc aggtggccga
     7321 gatggtcatc gagaaagcga aacgcctggt cgagcataag cgcgacgtgg tgatcctgct
     7381 cgattcgatc acgcggctcg gccgcgccta caacaccgtg gtgccttcgt cgggcaaggt
     7441 gctgaccggc ggcgtcgacg cgaacgcgct ccagcgcccg aagcgcttct tcggcgccgc
     7501 gcgtaacatc gaggagggcg gctcgctcac catcatcgcc accgctctga tcgataccgg
     7561 cagccgcatg gacgaggtga tcttcgagga gttcaagggc accggcaatt cggaaatcgt
     7621 gctcgaccgc aaagttgccg acaagcgcat cttcccggcg atcgacattg cgcgatcggg
     7681 cacgcgcaaa gaggaactcc tggtgcaggg cgatcagctc aagaagatgt acgtgcttcg
     7741 ccgcatcctg aacccgatgg ggatccagga cgcgatcgag ttcctgctcg acaagctgaa
     7801 gcagacaaag ggcaacagcg acttcttcga ctcgatgaac acgtaaggcg cgcgaagccg
     7861 ctccccgccg gttgccacgt gctcgagcct ttccacattg tcatggccgg cttggcccgg
     7921 ccatccacgt ctttggccct ggacggaaat cgtggatgcc cgggacgacg agcttgcgcc
     7981 cgggccggcg aagccggact cgggtgcctg ggcatgacgg cgctgcggtt cgacctacac
     8041 cgtccccacc tggcgggcgg ccttgacggt tgcgaccagt gccgcgggcg ccgtcaccgg
     8101 cagggagcat tgcgggccga tgcaaacata ggccgtcggc ttgccgtcga tcgaggtctt
     8161 gccgtgcgcc ggtgacgatg gcgaaagcgc gctcgcgcca ccctcccctt ccctaacctc
     8221 ctgcaccacg gcgttcggca gcgagacatc ggcaagcgcg cggcgcaact cccgcgcatc
     8281 gcggccctcc ggcaccaaca gcacgacgag cgccggcgcg taaacatcga gcgttgccgc
     8341 aagcatcccg gcatgggcga gcacgctttc gctcatggcg cccgcgaagc cgcttaggat
     8401 cgcttcagcg cggtcccggt agcgtgcctc tccggtccac agcaacagcg ccataagatt
     8461 cgacaccatc accgcattgg cgttcggggt ggcgtcgtcc tggccgctga acgggcgcac
     8521 gatgaggtcc gaggtatcgt cggcggcgaa atagtagcca ccaagatcct ccgcccagta
     8581 gtggcggtcg agcacgtcgg tccattcgcg ggcgcgctcg acatagctcg acttacccgt
     8641 gacactcgcg agagcgagcg cggcgtcgat catgttggcg tagtcgtttg cggtcgccgg
     8701 cgccttcgcc tcgcccgcgc gataggcgtg gaacagccgc ccatttgaga tcatccgtgt
     8761 acatacgaaa tcgaaggcgc gctccgcggc gtcgagccag tcaacgcggt cgaacgcgtc
     8821 cgccgcgttg gcgagtgccg cgatcatcaa tccgttccag tcggcgagca ccttgtcgtc
     8881 gaatccgggg cgcacgcggc tggagcgccg cgcgagcagc ttttggcgca tcgccgcaag
     8941 acgcgcttcc gtctcggcgt cgcgcaattc gatcgcgtcg agccggttga ggatgttgtg
     9001 gccttcgaaa tttccttgcg acgtcacgcc gtaaacttcg gcgaagagtt tcgcgtcctc
     9061 ggcgccgagc gcgtcctcga tcgcgggcaa gctccacaca tagaacttcc cctcctcgcc
     9121 ctcgctgtcg gcgtcgagcg acgaggcgaa agcaccaccc tcgaccgtca tctcgcgcat
     9181 cagccagtcg atcgtctccg cgatccgctc ggcgtagagc ggcgacttcg tctcgcgcca
     9241 gacctcggtc atcatggtga cgagctctgc gttgtcgtag agcatcttct cgaaatgcgg
     9301 cacgagccag cgcgcatcga cgctgtagcg cgcgaacccg ccgccgaggt gatcgtagat
     9361 cccgccttgg gcgattcgcg tcagcgtgag atcgaccgct tcgcgcggat gcgggagacc
     9421 gtagcgaagg ccggcgcgcc acaagaattc gaaaaattgc ggctgcggga attttggcgc
     9481 gccgctgatc ccgccataga ccggatcgac cgcttgcacg aagcggcggg cgagatcggc
     9541 gagctgcgcc tccggtggcg gcgcggcgcg aacgccgccg cggcgcggcg cgagccgctc
     9601 tttcagaatt tgcgcgttgt gccggacctt gtccttctcg gcgtggtaga tgcgcgagac
     9661 ttcattcagt acacggacga atggggggcg cccgaagcgc tcgtccttgg gaaagtaggt
     9721 gccgccccag aacggctcgg cctccgaggt gaggaacatg gtgagcggcc agccgccctg
     9781 ctcgccgagc gcatgcaatg cgcccatgta aatcgcatcg atatcgggac gctcctcgcg
     9841 atcgaccttg atgttgacga agaggtcgtt catcacggcg gccgtagcgt cgtcctcgaa
     9901 gctctcatgg gccatgacgt ggcaccaatg gcaggcggcg tagccgaccg agagcaggat
     9961 cggctttccc gtcgcgcgcg cctctttgag cgccgcatcg ccccagggac gccagtggac
    10021 cggattgtcc ttgtgctgga ggagataggg gctggtttcg cgggccagct cgttaccgtc
    10081 gacgcgctcc tgcatggtcc ttcctttcgc cgcgccctag gataacgcgt ttgccgcatg
    10141 gatggcctac tcgatggacg caaatcgcct cttccccctc accctttccg ccatgcgact
    10201 tgcgcagctt cgtgcgcaag atcgcggcag aaagccctct cccgcaaggg gagagggatt
    10261 atggggcgtg cccacgggta ccaacccctc tcccctcgcg ggagagagtg gcgctcaaaa
    10321 gagcgcgccg ggtgaggggg atttgcaagg cgagcggtca cgtctcgcgg ccgccacgcc
    10381 gatacggact caactcaagt ggcttcgatg atcaccgctg atgccgacac catcgtcgcc
    10441 ccggcgagcg gcgccggccg tgccgccatc gccgtggtcc gcatctcggg cgagagaacg
    10501 cgcgatgtcc tcgatgcgtt gtgtggcggc gcgccccctc cccgccgggc gagcttgcgc
    10561 gcaatcgggc tcgcaaacgc gccgatcgat cgcggcctcg tgctctggtt tccaggtcct
    10621 cagagcttca ccggcgaaga catggccgag ctccatgtgc atgggagccg cgccgtgatc
    10681 cgcgccgtca tcgatgcggt gctgtcgatc cccggaacgc gcctcgccga gccgggcgag
    10741 ttcgcgcgcc gcgccttcga gaagggcaag ctcgatctga cggaggtcga agggcttgcc
    10801 gatctcgtca acgccgagac ggaggcgcag cggcgccaag cgctcgcgca ggccgaaggg
    10861 tctttgcgcg ctctctacga gggctggcgc gcggagctcg tgcgggcgca ggcgctgatc
    10921 gaagcgggac tcgacttcgc cgacgagggc gacgtgatcg ccgacgtcag cgcaaaagcg
    10981 gaccccatcg tcgcgcgtct cttagccgcg atcgcgcacc atctgttgga tcgtcgtgga
    11041 gagcgtttac gcgatgggtt caaggtcgtc atcgccggcg ctcccaatgc cggcaagtcg
    11101 agcctcctga acgcgctcgc caagcgcgac gccgcgatcg tgtccgagga agccggcacg
    11161 acgcgcgatg cgatcgaggt ccatctcgat ctcggcggcg tgccggtcat gctcatcgac
    11221 acggcgggct tgcgcgcggc cgaaggcaag atcgaggccg aagggatcag gcgcgccttg
    11281 gcgcgggcag gggacgccga cctcgttctg tgggtggtcg atgcgaccgc gccgcaatgg
    11341 catgcgcctt tgggaattgg tctgggcaag tcgggtgaag ccgatgcggc ggcggtcacg
    11401 gtcctgaaca agatcgacct cgcgcccggg gccggcgcag gcacggattg cctcaagctc
    11461 tcggcgaaga cgggcgaggg gatcgaacac cttgtcgacg cgctcgccga gaagacgact
    11521 cttgcggcgg agcaaagggc gggggctccg ctcctgacgc gtacacgtca cagggtcgag
    11581 cttgaaggcg cgcgtgcggc cctcgagcgc tatggcgatg cagctctcgg tccagagttc
    11641 aaggccgagg agctgcggat cgccgcccgg catctcggca ggctcaccgg cctgatcgat
    11701 gtcgaggagg tactgggcgc gatcttcgcc gagttctgca ttggcaagta agagggcgct
    11761 ctgattcacg tgaaacaatt ggggcggctg ctttgacgaa cgcgcgcgaa ccgactaggt
    11821 acggcccatg aacaagggct tcgacgtgat cgtgatcggc ggcggccatg ccggctgcga
    11881 ggccgcgtcc gcgtccgcgc gcatgggcgc agcaacagcc ctcgttaccc acagtttttc
    11941 caccatcggc gagatgtcct gcaatccggc catcggcggc ctgggcaagg gccatctcgt
    12001 gcgcgagatc gacgcgctcg acgggctgat gggccgcgcg attgatcgcg gcggcatcca
    12061 gttccgcatg ctgaaccgca gcaagggacc ggccgtgcgc ggcccgcgc
//
LOCUS       DASUQP010000049         3531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1793146, whole genome shotgun sequence.
ACCESSION   DASUQP010000049 DASUQP010000000
VERSION     DASUQP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793146"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /gene="cysW"
                     /locus_tag="VFY74_02280"
     CDS             complement(<1..562)
                     /gene="cysW"
                     /locus_tag="VFY74_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928479.1"
                     /note="Part of the ABC transporter complex cysAWTP
                     involved in sulfate/thiosulfate import; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HEX5930567.1"
                     /translation="MSGVALGTDVVTEDRARSVTTERRPVRVALILIAVGFLILFLLL
                     PLVAVFAEALRAGFGGYWQAISEPDAVAAMKLTLLVAGIAVPANLIFGLAASWAIAKF
                     EFPGKSILNTLIDLPFSVSPVISGLVYVLLFGLQGYFGVYLQSQGIQIIFAVPGIVLP
                     TIFVTFPFVARELVPLMQEQGTQDEEA"
     gene            complement(559..1404)
                     /gene="cysT"
                     /locus_tag="VFY74_02285"
     CDS             complement(559..1404)
                     /gene="cysT"
                     /locus_tag="VFY74_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX5930568.1"
                     /translation="MAGARGLALKRPSALPGFGLALGFTLTYLSLIVLLPLAALAIKA
                     ASLGASGFWEIVTASRTMAALRISFVNSFIAAAINVVFGVIVAWVLVRYRFPGRRLLD
                     AVVDLPFALPTAVAGIALTAIYARNGWIGGLLEPYGVKIAYTPWGIGVALVFIGLPFV
                     VRTVQPVLEDFEREIEEAAASLGASRRQTVFRVMMPALLPAILTGFVLAFARAVGEYG
                     SVIFIAGNIPFVSEIAPLLIVIRLEEFGYAQATAIAAAMLAIAFVVILLVNLIQAWQR
                     RRFGT"
     gene            complement(1417..2439)
                     /locus_tag="VFY74_02290"
     CDS             complement(1417..2439)
                     /locus_tag="VFY74_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5930569.1"
                     /translation="MTTLRNTLAAVAALGLALGAGAAIADTSLLNVSYDPTRELYKEV
                     NAAFIKDWKAKGGADVTIEQSHGGSGKQARAVIDGLQADVVTLALQGDIDQIAKAGLV
                     GADWQKRLEHNASPYTSTIVFLVRKGNPKNIKDWDDLVKEGVEVITPNPKTSGGARWN
                     YLAAWAFADGKYGHDQSKNQDFIRKLYANVPVLDTGARGSTTTFAQRGIGDVLLAWEN
                     EAFLAIDELGADKFEIVVPSLSIKAEPPVAVVDKVVDAKGTRKVAEAYLKFLYTPEAQ
                     KLIAKRYYRPSDRSAADAKDLARFAKLNLVSIDDPLFGGWAAAQPKHFDDGGIFDQIY
                     QPKSTN"
     gene            complement(2587..3048)
                     /locus_tag="VFY74_02295"
     CDS             complement(2587..3048)
                     /locus_tag="VFY74_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5930570.1"
                     /translation="MISQKARYALRALIALAGTDSMMIAEIAGQQRIPRKFLEQILLD
                     LKRHGIVASRRGRLGGYALLARPDQITFGRILRIIDGPIAPLPCLSRIAYRRCPDCKN
                     ESTCEIRRVFAGVAESARAVLDRTTIAEAIGGDRMKPGRPPRKRAAARKGA"
     gene            complement(3154..3282)
                     /locus_tag="VFY74_02300"
     CDS             complement(3154..3282)
                     /locus_tag="VFY74_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930571.1"
                     /translation="MSPPRETPWKGIAIVLAILLGAVVIAAALIVGGIMQLGFSLL"
BASE COUNT          714 a         1104 c         1127 g          586 t
ORIGIN      
        1 cggcttcctc gtcctgcgtc ccctgctcct gcatcaacgg gacgagctcg cgcgcgacga
       61 aagggaaggt tacgaagatg gtggggagca cgatgcccgg gacggcgaag atgatctgga
      121 tgccttggct ttgcagatag acgccgaaat agccttgcag cccgaacagg agcacgtaga
      181 cgagccccga gatcaccgga gacacggaga acggcaggtc gatcagcgtg ttcaggatgc
      241 tcttgcctgg aaactcgaac ttggcgatcg cccaggatgc ggcaagcccg aagatgagat
      301 tggcgggcac cgcgatgccg gcgacgagga gcgtgagctt catggccgcc acggcatcgg
      361 gctcggagat ggcctgccaa tatccaccga acccggcgcg caacgcttcg gcgaacacgg
      421 cgacgagcgg caggagcagg aacaagatca aaaagcccac ggcgatgagg atgagcgcga
      481 cgcgcacagg ccgccgctcc gtggtcaccg atcgggcgcg gtcctcggtg acgacatcgg
      541 tgccgagagc gacgccgctc atgtcccgaa cctcctgcgc tgccatgctt ggatgagatt
      601 gacgagtaga ataacgacga aggcgatggc gagcatggcg gctgcaatcg ccgtcgcctg
      661 tgcatagccg aactcttcca gcctgatgac gatcaggagc ggcgcgatct ccgagacgaa
      721 gggaatattg ccggcgatga agatcaccga gccgtactcg ccgacggccc gggcgaaagc
      781 gagcacaaag ccggtgagga tggccgggag cagcgcaggc atcatcaccc ggaacacggt
      841 ctggcgccgg ctcgcgccga gcgaggccgc agcttcctcg atctcgcgct cgaaatcctc
      901 gagcacgggc tgcacggtgc gcaccacgaa cggcaagccg atgaacacca gcgccacgcc
      961 gatgccccaa ggcgtgtagg caatcttgac gccgtagggc tcgagcaggc cgccaatcca
     1021 gccgttccgc gcatagatcg ccgtgagcgc gatgccggcc accgccgtgg ggagcgcgaa
     1081 tggcagatcg acgacggcat cgagaaggcg gcggccggga aagcgatagc ggaccagcac
     1141 ccaggccacg attacgccga agacgacgtt gatggccgcg gcgatgaagg aattgacgaa
     1201 gcttatgcgc agcgccgcca tcgtgcgcga cgccgtgacg atctcccaaa agccgctcgc
     1261 tcccagcgac gccgccttga tggcgagagc ggcgagcggg aggagaacga tcaggctcaa
     1321 atacgtcaaa gtgaaaccga gcgccaaccc gaagccgggt aaggcgctcg gccgcttcaa
     1381 cgccaggccc cgggcgccgg ccatgagcgt gtccctctag ttggtcgact tcggctgata
     1441 gatctgatcg aagatgccgc cgtcgtcgaa gtgtttcggc tgggcggcgg cccatccgcc
     1501 gaacagagga tcgtcgatcg ataccaagtt cagcttggcg aagcgggcaa gatccttcgc
     1561 atcggccgcg ctccggtcgg agggacgata atagcgcttg gcgatgagct tctgcgcttc
     1621 cggcgtatag aggaacttca gataagcctc ggccactttc cgggtgccct tggcgtcgac
     1681 gaccttgtcg accacggcaa ccggcggctc cgccttgatc gagagcgacg ggaccacgat
     1741 ctcgaacttg tcggcgccga gctcgtcgat ggcgaggaag gcctcgttct cccaagcgag
     1801 caggacgtcg ccgatgccgc gctgggcaaa ggtcgtggtg gagccgcggg cgccggtgtc
     1861 gagcacaggc acgttggcgt agagcttcct gataaagtcc tggttcttgc tctggtcatg
     1921 gccgtacttg ccgtcggcaa aagcccaggc ggcgaggtag ttccagcggg cgccccccga
     1981 agtcttcggg ttcggcgtaa tcacttccac gccctctttg acgaggtcat cccaatcctt
     2041 gatgttcttc gggttccctt tgcggaccag gaacacaatg gtcgaggtgt agggggaagc
     2101 attgtgctcg agccgcttct gccaatcggc gccgaccaga ccggccttgg cgatttgatc
     2161 gatgtcgccc tgcagcgcga gcgtcaccac atcggcttga aggccgtcga tcacggcgcg
     2221 ggcctgtttg cccgatccgc catgtgactg ctcgatcgtc acatccgctc cgcccttcgc
     2281 cttccaatcc ttgatgaagg cggcattcac ctccttgtag agctcgcggg tcgggtcata
     2341 ggacacattg agaaggctgg tgtcggcaat tgcggccccc gcgccgagcg cgaggccgag
     2401 cgcggctacg gccgcaaggg tgtttcgcag tgtcgtcatc ggagcatctc ctctgttgaa
     2461 atacctaaaa ctctatcatc taagtagtat tttagcaagc aaagtccctt gctttatttt
     2521 ccaggagaag caaatgcccg tcgcgctaaa ccaccggccc gcgaaaaaat ttccccacac
     2581 gcgccgtcag gcgcccttcc tggccgcagc cctcttccgg ggcgggcggc ccggcttcat
     2641 gcggtcaccg ccaatcgcct cggcaatcgt cgtgcggtca agaacggccc tcgccgactc
     2701 tgccacgccg gcgaacaccc gacggatctc gcaggtggac tcattcttgc agtcggggca
     2761 gcggcgatag gcaatccggc tcaggcaagg aagcggcgcg atgggcccgt cgataatcct
     2821 gagaatccgg ccaaaggtga tctgatcggg ccgggccagc aaggcatagc cgccgagtcg
     2881 gcctctgcgg ctcgccacaa tgccgtgccg cttcaagtcg agcaggattt gttcgaggaa
     2941 tttgcgcggg atgcgttgct gcccggcaat ctccgcgatc atcatcgaat cggtcccggc
     3001 gagcgcgatg agcgccctca gggcatagcg tgccttctgt gagatcatgg ccgaggaaaa
     3061 agccctaaag ccgacggact taatagaata tccggtgcaa aacaagcccg cgcgagacgc
     3121 gcaggcgcaa aatagatcaa ccgcgattga cgcttagagc agcgaaaacc cgagctgcat
     3181 gatgccgccc acgatgagcg cggccgcgat cacgaccgcg ccgagaagga tcgcgagcac
     3241 gatggcaatg cctttccatg gggtttctcg tggcgggctc atggtgtcat tattctcggt
     3301 aaaagccccc tgcaaggaag gcggccaagc tcgcggagcc tgacggcctc tttatctatc
     3361 ggtccaaccg cgtcctagca ggggcggcac ctgcaccgga tgggacggca ggtccaactc
     3421 cgatcttccg cattcctcca tccgatctgc ctttgaggcg ggggcggaga tgaaaatagc
     3481 gccgaccccc gcgtcgcgaa agagacgcga ccggcgtccc ctttctgacc c
//
LOCUS       DASUQP010000050         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1793603, whole genome shotgun sequence.
ACCESSION   DASUQP010000050 DASUQP010000000
VERSION     DASUQP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793603"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..387)
                     /gene="rpmF"
                     /locus_tag="VFY74_02305"
     CDS             complement(202..387)
                     /gene="rpmF"
                     /locus_tag="VFY74_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5930572.1"
                     /translation="MAVPKRKVSRMKRGNRRSHDRLQAPAYVEDKDSGELRRPHHIDL
                     KSGRYRGRQVLDVKEET"
     gene            complement(497..1228)
                     /gene="mtgA"
                     /locus_tag="VFY74_02310"
     CDS             complement(497..1228)
                     /gene="mtgA"
                     /locus_tag="VFY74_02310"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX5930573.1"
                     /translation="MAVQAVSGERTRERSMRRKKHPWLRRLARILLVLAAILLLSIVA
                     FRFINPPITPVMLVEKLRGATLRHAWVPLEDMSRLLPLAAISSEDGRFCFHWGVDWGA
                     VKEAIDEGGSFGAFRGASTIPMQTAKNLYLWSGRSYVRKALEVPLAYLMSALWPKARM
                     MEVYLNVAQFGRGIFGVEAASRYYFNKSAAELTRRETILLVAALPNPRFRNPAKPSRH
                     MLRVARAIEARLPIMAPRSACVLPR"
     gene            1362..2225
                     /locus_tag="VFY74_02315"
     CDS             1362..2225
                     /locus_tag="VFY74_02315"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5930574.1"
                     /translation="MAGAVERRLDRFLAKSDVPPGGARLGQAMRHATIGGGKRLRPFL
                     LVESARLFGVRDEEALPAATALECIHCYSLVHDDLPSMDDDAVRRGRPTLHIAFDEAT
                     AILAGDALLTLAFEILSDDSTHPEAAIRAELVLLLAKAAGWKGMAQGQVLDLSAERQG
                     FGPAEIAAMQALKTGALFGFACEAGAVLGQASPSERAALTAYASAFGQAFQLADDLLD
                     AEGDASALGKAVAKDARRGKATLVARLGPDDARKRLGTLLQDAEAALAPFADRAETLR
                     ELARFIATRKA"
     gene            2258..2965
                     /locus_tag="VFY74_02320"
     CDS             2258..2965
                     /locus_tag="VFY74_02320"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX5930575.1"
                     /translation="MAKPKQTRIESHHRGKLRPWLVRFVLAHKRLFGSIAIGLLVFLL
                     LPKVGLPVPRGFFAWDVGVICFLASVYWMVWHSDAAMIRRQSATQDEGRNLVLIGAIG
                     AVVISVAVILVWLSGEMSAGRPPARDLALVFLTIPLSWVFIHTIFMLHYAHEFYAEHR
                     GAGGGLRFPGDDQPDYWDFVYFAFGVGMAAQVADVTVTSKPVRHIVMIHSVVSFIFNV
                     TLIAMTVSIIGDAISTR"
BASE COUNT          580 a         1144 c         1077 g          636 t
ORIGIN      
        1 ctggtgcgaa cggcttgcgg ccactccggc acgaacatcg aatacgtgct gaacacggtg
       61 cgccatctcg aagaggcggg cattcatgac gttgagctcg cggcgctggc aaagcgcctc
      121 accgcgcgac cgggcggcgg gagctagtct gcatggaggt ctgcccggtc aggcgtcacg
      181 acgcgggccg cacgggggcg cttacgtctc ttccttgacg tcgagcacct ggcggccgcg
      241 atagcggccc gatttcaggt cgatgtgatg cggccgtcgc agctcgccgg agtccttgtc
      301 ctcgacatag gcgggagcct gaagccgatc gtggctccgt ctgttgcccc gcttcatgcg
      361 cgagactttt cgtttgggaa cagccattgt tctaagaccc tatgaacgga cccgcgggca
      421 agcgcgtctt cgagcccgcc cttatagtgg caatctttcg ggctggccag gggccggagc
      481 agaaaaggca gcgccactat cgaggcagga cgcaagccga gcgcggagcc atgatcggca
      541 ggcgggcctc gatcgccctc gccacccgca gcatgtgccg gctcggcttt gccgggttcc
      601 tgaaccgcgg gttcggcagc gccgcgacca gaaggatcgt ctcgcggcgg gtaagctctg
      661 ccgcgctctt gttgaaataa tagcggctcg cggcttcgac gccgaagatg ccgcggccga
      721 actgggcgac attgagatag acctccatca tccgcgcctt gggccacagc gccgacatca
      781 gataggcgag cggcacctcg agcgccttgc gcacgtagct tcgcccgctc caaagataga
      841 ggttcttggc ggtctgcatt ggaatggtgc tcgcgccgcg gaaagccccg aaactcccgc
      901 cttcgtcgat ggcttctttc accgcacccc aatcgacgcc ccaatgaaag caaaaacggc
      961 cgtcctcgct cgaaatcgca gccagtggga gcagccgcga catgtcttcg agcggcaccc
     1021 acgcgtggcg gagcgtggcc cccctcaact tctccacgag catgaccggg gtgatgggtg
     1081 gattgatgaa acgaaaggcg acgatggaaa ggagaaggat tgcggcgaga accaacaaaa
     1141 tgcgcgccag gcgccggagc cagggatgct tctttcgcct catgctgcgc tcccgcgtgc
     1201 gctcgcctga gaccgcttga accgccatga cttggccttg tacctccggg ttgccgcctc
     1261 aagcaacgtc gtattagggt aacgattacc gatccttttg cggcaaagcg ccgctggtgt
     1321 ttcgctttcc ccgcatggcc tttctccaaa gactcgaacg cgtggcgggc gccgtcgaga
     1381 ggcgcctcga ccgctttctc gccaagtccg acgtcccgcc ggggggcgcg aggctcgggc
     1441 aggccatgcg ccatgccaca atcggcggcg gcaagcgctt gcggccgttc cttctcgtcg
     1501 agtcggcgcg gctatttggc gtgcgggacg aagaggccct gcccgccgcg accgcgctcg
     1561 aatgcatcca ttgctactcg ctcgttcatg acgacttgcc gtccatggac gatgacgcgg
     1621 ttcgccgcgg gcgtccgacc cttcacatcg ctttcgatga ggcgacagcc atcctcgcgg
     1681 gcgatgcgct tctcacgctc gccttcgaaa tcttgagcga cgacagcacc catcccgagg
     1741 ccgccattcg cgccgagctt gtcctccttc tcgccaaggc cgctggctgg aagggcatgg
     1801 cgcagggaca ggtgctcgat ctcagcgccg agcggcaagg cttcggcccc gccgagattg
     1861 ccgccatgca ggcgctcaag acaggcgccc tgttcggctt cgcctgcgag gcgggggcgg
     1921 tactcgggca ggcctcgcct tcggaacgcg cggccctcac cgcctatgcc tccgccttcg
     1981 gccaggcctt ccagctcgcc gacgatctgc tcgacgctga aggcgatgcc tcggctctcg
     2041 gcaaggccgt cgccaaggac gcacgccgcg gcaaggccac gctcgttgcc cggcttgggc
     2101 ccgatgacgc gcggaagcgg ctgggtactt tgctgcagga cgccgaagcc gcgcttgcac
     2161 ccttcgccga tcgggcagag accttgcggg aattggcccg cttcatcgcc acccgcaagg
     2221 cttgaataaa agggcgttca ggactccaat tctcgtcatg gccaagccca agcagaccag
     2281 aattgaatcg caccaccgcg ggaagctgcg cccgtggctg gtccgcttcg tgcttgccca
     2341 caagcgcttg ttcggcagca tcgcgatcgg acttctggtc ttcctgctgc tgccgaaagt
     2401 tgggctgccg gtgccacggg gcttcttcgc ctgggacgtc ggcgtgattt gttttctcgc
     2461 atcggtctat tggatggtct ggcactcgga cgcggccatg atccggcggc aatcggcgac
     2521 gcaggacgag ggccgcaatc tcgtgctcat cggcgcgatc ggcgccgtcg tcatcagcgt
     2581 cgccgtgatc ctggtttggc tcagcgggga gatgagcgcg ggccgaccgc ccgcgcgcga
     2641 tctcgcgctc gtgtttctca ccattccgct gtcatgggtc ttcattcaca ccatcttcat
     2701 gctgcactac gcgcatgagt tctacgccga gcatcgcggc gccggcggcg gcttgcgctt
     2761 ccccggcgac gaccagcccg actattggga cttcgtctat ttcgccttcg gcgtcggcat
     2821 ggcggcgcag gtggccgatg tcacggtgac gtcaaagccg gtccgccaca tcgtgatgat
     2881 ccacagcgtc gtctcgttca ttttcaacgt caccttgatc gccatgacgg tgagcattat
     2941 cggcgacgct atttccaccc gctaaggcgc tccgcgcgca ggattcgccg gcgcctgctg
     3001 ggcaggaaca gcgcatcctc ggggttttgg cgagcatcat acaggcgccg agatcgacgc
     3061 cgggcacgac cgcctgatgc tcatccgtgc cgcgcgaggc aagtctcctg actatttggg
     3121 attgccctcg gcgagcgcgc ccgcgccctc agcaagagcg cgacaagcgc atgcaccccg
     3181 tgcgcatcaa gcgtctaatt ggtcttgccc gagccaaggg tagacacacg agcatcgcac
     3241 caatcttgga cgcgcgtgca ccactgcgac aacttctgtg cgcaacttcg caagtcatcc
     3301 aggtaggctt gaggattcgc gctagtgcgc gccctgtctg ggacccagcc cgcataaagt
     3361 tttacgtcgg caagaccaca gggcctgtca ggactccgac gcttggcttc gaaaacccaa
     3421 tgacctgtag gaactcg
//
LOCUS       DASUQP010000051         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_294943,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000051 DASUQP010000000
VERSION     DASUQP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294943"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VFY74_02325"
     CDS             complement(<1..131)
                     /locus_tag="VFY74_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX5930576.1"
                     /translation="MVALDRLARSTSDLYRIISDLTAKGVAFKVLDDPSIDTTSRTG"
     gene            179..652
                     /locus_tag="VFY74_02330"
     CDS             179..652
                     /locus_tag="VFY74_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930577.1"
                     /translation="MIANAGSRRRRMAVAPGLALIVGFGLAVASQPALAGLVENERSV
                     SLPARTGAQADTPIIVAEKYKNKNWNKNKNWNKNWNKHYSNKNYYVNKNRGYVRHWKH
                     RPYYGQFFGGIVLGSILTAAIAGVPPPPPRPDLCWYWSDPYMYRGYWDYCYYPGY"
     gene            complement(723..1109)
                     /locus_tag="VFY74_02335"
     CDS             complement(723..1109)
                     /locus_tag="VFY74_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930578.1"
                     /translation="MGAQHFVRFLPSRDPNPLAGLITISPRNDSQEVAIRTAKEPEFT
                     TEQIGWRFALGTVIIVGSYGAWPLIPVVLTADLTPGVKAALSGLFGATPFLSKLVAIA
                     LMGRPAYYFLKRTLFRRLRRSANAAK"
     gene            complement(1167..1574)
                     /locus_tag="VFY74_02340"
     CDS             complement(1167..1574)
                     /locus_tag="VFY74_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX5930579.1"
                     /translation="MADVRAMCAIGLRGQLGLNGRLPWEGNKGREFMADVARFFDVTR
                     GHVIIAGPTTIASIPAFAYEDRTIRDIRSTDHPAEVLASFPGRVVYVGGGPPVWEAYA
                     PLIRHWDITRLPYDGEADRWFNPAWLIASGKPA"
     gene            1668..2609
                     /locus_tag="VFY74_02345"
     CDS             1668..2609
                     /locus_tag="VFY74_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5930580.1"
                     /translation="MTPRARPLSHADESHRVKAIGLMVLATLCFAALDATGKYLIAEQ
                     GVPIIEVSWLRFLGHVVFNAVVLWPLALKPSLRSAKPVMQILRSVLMVLTTGLNFVAL
                     KYLQLDQNITIFFLTPLLVAALAGPLLKEWVGWHRMLAIIAGFLGVLLVMHPGIGTVH
                     WAMFIALGAMLGYALYNIATRYLAAFDPASVTQTYSPLAGVILLAPPALAEWHWPTEP
                     LQLLMLASLGFWGGLGHWFLILAHRGAPAPVLAPFIYVGLIWMSALGFLVFGDVPTMW
                     TLSGAAVVILSGLYLLARERKVLGAAKAGPASDIATQ"
     gene            complement(2627..>3539)
                     /gene="tgt"
                     /locus_tag="VFY74_02350"
     CDS             complement(2627..>3539)
                     /gene="tgt"
                     /locus_tag="VFY74_02350"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5930581.1"
                     /translation="KLGGLHRFMHWEKPILTDSGGYQVMSLAKLRRIGEEGVTFQSHI
                     DGVSVTLSPERAIEVQCLLGADIQMVLDECTPFPATREEAKTSLDLSLRWAERSKAAF
                     GADAKPGQALFGIVQGSVYPDLRERSAQALLAIGFDGYAIGGLAVGEGQATMLDMVEV
                     TTAILPQDPPRYLMGVGTPEDLLAAIARGIDMFDCVMPTRSGRHCQAFTWGGKLNLRN
                     ARYADDPVPLDPESACPAAQYERAYLHHLVKSGETLGAMLLSYANVQFYQELMARARE
                     AIAQGRFASFVEDLRRRYAKANDKDDG"
BASE COUNT          629 a         1094 c         1123 g          693 t
ORIGIN      
        1 ttgccagtgc gggaggtcgt gtcgatgctg ggatcgtcca gcaccttaaa ggccaccccc
       61 ttcgcggtga gatccgagat gatgcggtag aggtcgctgg tggagcgcgc caggcggtcc
      121 aatgccacca tggcttgcaa ttgattcgtt aggatcgcga atcttggagg aaaaccctat
      181 gattgctaac gcaggttcac ggcgccggcg gatggctgtg gcgcccggcc ttgccctcat
      241 cgtcggcttc ggcctcgccg ttgcatcgca accggccctg gctggcctcg ttgagaacga
      301 acgaagcgtt tcactgccgg cccggaccgg ggctcaggcg gataccccga tcatcgtggc
      361 cgaaaagtac aagaacaaga actggaataa gaacaagaat tggaataaga actggaacaa
      421 gcactactcg aacaagaact attacgtcaa taaaaacaga ggttacgtgc gacattggaa
      481 acacaggccc tattacggcc agttcttcgg ggggatcgtg cttgggtcga ttctgaccgc
      541 tgcgatcgcc ggcgtcccac cgcctccgcc acgccctgac ctctgttggt actggtcgga
      601 tccctacatg tatcggggct attgggatta ctgctactac ccgggctact aggcgacgtt
      661 catcgcgctc ttgcaagggc gccggtttga cggcgccctt ttgcgcgtcg gcatttgcgc
      721 ggctatttgg cggcgttggc agaccggcgc agccggcgga acaaggtgcg cttgaggaag
      781 tagtaggcgg gacgtcccat cagggcgatg gcaacgagct tggagaggaa gggcgtggca
      841 ccgaacaggc ccgataaagc agccttcacg cccggcgtca ggtcggccgt gagcaccacc
      901 gggatcagcg gccaggcgcc gtaactcccg acaatgatga ccgtgccgag ggcaaatcgc
      961 cagccgatct gctcggtggt gaattccggc tcctttgcgg tcctgatggc gacctcctgg
     1021 gagtcgtttc ggggtgatat cgttataagc cctgccagag gatttgggtc tctcgaaggc
     1081 agaaagcgca cgaaatgctg tgcccccaat atggtagcct cggcaggctc ggatacgaaa
     1141 gaaaaatcac ccgattttgc gccgatctag gccggctttc ccgaagcgat gagccaggca
     1201 ggattgaacc agcgatcggc ctcgccgtca tacgggagcc gggtgatgtc ccaatgacgg
     1261 atcaacgggg cgtaggcctc ccagaccggc ggaccgccgc cgacatagac gacacggccg
     1321 gggaacgagg ccagtacttc ggcggggtgg tctgtcgaac ggatatcgcg gatggtgcgg
     1381 tcttcatagg caaaggccgg gatggaggcg atggtggtgg ggccggcgat gatcacatga
     1441 ccgcgggtga cgtcgaagaa ccgcgcgacg tcggccatga attctcggcc cttgtttcct
     1501 tcccaaggca gcctgccatt gagcccgagc tgcccgcgca ggcctatggc gcacatagcg
     1561 cgaacgtcag ccacgcactc ctccattcgc taatgccaca gcgaatgcgt ctcgcgacct
     1621 taaccgccga tggccttttg gcaaagattg atgcagaaac ccctcccatg actccccgcg
     1681 ctcgccccct gtcccacgcc gatgagagcc atcgcgtcaa ggcgatcggt ctgatggtgc
     1741 tcgcgacgct ctgctttgcc gcgctcgacg ccacgggcaa gtatctcatt gccgagcaag
     1801 gagtgccgat tatcgaggtg tcgtggctca gattcttggg ccatgtggtg ttcaacgccg
     1861 tcgtgctgtg gccgttggcg ctcaagccat cgctccgatc ggccaaaccc gtgatgcaga
     1921 ttcttcgctc cgtgctgatg gtcctgacca cgggtctgaa tttcgtggcg ctaaagtatc
     1981 tccagctcga ccagaacatc accattttct ttctcacccc tcttctcgtc gcagccctcg
     2041 ccgggccgct gctcaaggag tgggtcggct ggcatcgcat gctggccatt atcgcgggct
     2101 tccttggcgt gctcctggtg atgcatcccg gcatcggcac ggttcattgg gcgatgttta
     2161 tcgcgctcgg tgcgatgctc ggctacgcgc tctacaatat cgccacgcgt tatctcgccg
     2221 cgttcgaccc cgcctcggtg acgcaaactt attcgccgct tgcgggcgtt atcctcctgg
     2281 cgccgcccgc cctcgcggaa tggcattggc cgacggagcc cctgcaattg ttgatgctcg
     2341 cctcgctcgg gttttggggc gggctcgggc actggttcct gatccttgcc catcgcggcg
     2401 caccggctcc ggtgctcgcg cccttcatct atgtggggct catctggatg tcggcgctcg
     2461 gctttctcgt gttcggcgac gtgccgacga tgtggacgct ctccggcgcc gccgtcgtca
     2521 tcttgtcggg cctctatctt ctcgcacgcg agagaaaggt gctcggcgcg gcaaaggcgg
     2581 gaccggcctc ggatatcgcc acgcagtgac gtgcgccgcg cgggcgttag ccgtcgtcct
     2641 tgtcgtttgc tttggcgtag cggcgtcgca aatcctccac gaagctcgcg aaacgtcctt
     2701 gcgcgatcgc ctcgcgagca cgcgccatca gctcctggta gaactgaaca ttggcgtagc
     2761 tcaagagcat ggccccgagc gtttcgcccg atttcaccag gtgatgcaga taggcgcgtt
     2821 cgtattgcgc ggcgggacag gcgctttccg gatcgagcgg cacggggtcg tcggcatagc
     2881 gcgcattgcg gagattgagc ttgcctcccc aggtgaaggc ctggcaatgg cgccccgagc
     2941 gggtcggcat gacgcaatcg aacatgtcga tgccgcgcgc gatcgcggcc agcagatcct
     3001 cgggcgtgcc gacgcccatg agatagcggg gagggtcctg cggcaggatc gctgttgtga
     3061 cctcgaccat gtcgagcatg gtggcctgcc cctcacccac ggcaagaccg ccaatggcgt
     3121 aaccgtcgaa gccgatcgcg agcagcgcct gcgcgctcct ctcgcggaga tcgggataga
     3181 cgcttccctg cacgatgccg aacaaggctt ggccgggctt ggcgtccgcg ccgaacgcgg
     3241 ccttgctgcg ctcggcccag cgcagcgaca ggtcgagcga cgtcttggct tcctcgcggg
     3301 tcgcaggaaa aggcgtgcac tcgtcgagca ccatctggat gtcggcgccg agcaggcatt
     3361 gcacctcgat ggcgcgctcg ggactgagcg ttaccgaaac gccatcgata tgggactgaa
     3421 aggtgacgcc ctcctcgccg atgcggcgca gcttcgccaa ggacatgacc tgatagccgc
     3481 ctgaatcggt gaggatcggc ttctcccaat gcatgaaacg gtggaggccg ccgagcttg
//
LOCUS       DASUQP010000052         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1657816, whole genome shotgun sequence.
ACCESSION   DASUQP010000052 DASUQP010000000
VERSION     DASUQP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657816"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFY74_02355"
     CDS             <1..193
                     /locus_tag="VFY74_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930582.1"
                     /translation="MHPLEDTMNGTVHNYRVEGARYGVSFGTALAIAISYVNNQSILW
                     AIIHGFLSWLYVIYFVLFK"
     gene            356..622
                     /locus_tag="VFY74_02360"
     CDS             356..622
                     /locus_tag="VFY74_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930583.1"
                     /translation="MKNTLGGSACKAALAVGAIAAITAMSGSASAHWHGGCWGCGAFA
                     AGAIAGAALASPYVAPRYYYGGPVYAGPRYPVCPVYGPRPYYCR"
     gene            703..945
                     /locus_tag="VFY74_02365"
     CDS             703..945
                     /locus_tag="VFY74_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930584.1"
                     /translation="MTTCVRLIAAAAVSIAASLPVLAEEAKTDSEAQPIPTLHCEFKA
                     VNACTADGTCKAGDNLAGMPLPLKVPVDFENGGVGD"
     gene            932..1135
                     /locus_tag="VFY74_02370"
     CDS             932..1135
                     /locus_tag="VFY74_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930585.1"
                     /translation="MSAIDDTGYARTDNIDAVADSAGQLIVHGLDRAFGWQIVIHHTS
                     PAASVSFASADSVIAGFGTCSHE"
     gene            1284..2531
                     /locus_tag="VFY74_02375"
     CDS             1284..2531
                     /locus_tag="VFY74_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930586.1"
                     /translation="MSVGGQQVRHRCGLSQRRLALVAFAAAISLIGANAAWAGPYEQS
                     PSFTATQVLSPNLLNSPNYTIGNQVGLDNFQYVFDVDTRWGTYSVKGSDLMRVRAREI
                     AATAALERIDSAGTLVNAAGRTALKPLATAKDLVTAPGRTIGDTVRGVGNLFGSVDAA
                     VGATDPHKERVIASVTGGATARRKLAYDFGVDPNTSFPPLHDELTRLATANAIGETST
                     NAGLAFVTGGAGIAISVGGTSRTLRLALRDKTAAQLEKEGRQLLATMGVPAATTNAFY
                     ANPHLSPTDKAIIVAALMNLGATAGREIFIASAADTQSIEMGFFYRRQAELIASFDKK
                     VAPVTAFVRVGGAPMLQTGKGTVSILPVDYLYWSPPLEGLIAGAGGGGQMWITGRASE
                     RARAQLAARGWTLVPKAGGKLAD"
BASE COUNT          463 a          876 c          861 g          475 t
ORIGIN      
        1 catgcaccct ttggaggata cgatgaacgg aacggtccac aattaccgcg tggaaggcgc
       61 gagatacggc gtcagcttcg gcacggcgct cgccatcgcc atctcatacg tcaacaacca
      121 gtcgattcta tgggcgatca ttcacggctt cttgagctgg ctttatgtga tctatttcgt
      181 gctattcaaa tagcgggaac ggggcactga gacgtgcctg ccgtcacgat gatgtcttcg
      241 tgttttggtt aacattggcg gcgcaagggc ggaagcgccg ccgaatcgtg ctggacttcg
      301 gcccgcgcgc gcttttcaat ccccgctcca tgacaatgca aggaggtacg acaccatgaa
      361 aaacacattg ggaggatcgg cttgcaaagc ggcgctcgcc gttggcgcca tcgcagcaat
      421 aaccgcgatg agcggcagcg cttcggctca ctggcacggc ggctgctggg gatgcggagc
      481 gtttgcggcc ggcgccatcg ccggtgcggc gctcgcgtcg ccttatgtgg caccgcgcta
      541 ctattacggc ggtccggtgt atgcggggcc gcgctatccg gtctgtccgg tgtacggtcc
      601 gcggccctat tactgccgct gaccgacgcc acgcgcctac ccccgttttc atccgacttc
      661 gatcaatgtt ggcgtggctc ccgcgcgcca gaggagacca ttatgaccac atgcgtcaga
      721 ctgattgccg ccgccgcggt ttcgatcgcg gccagcctgc cggtgcttgc cgaggaagcg
      781 aagaccgatt ccgaagccca gccgatcccg accctgcatt gcgagttcaa ggccgtgaat
      841 gcctgcacgg ctgacggcac gtgcaaggcc ggagacaatc tcgccggcat gcctctcccg
      901 ctcaaggtcc ccgtcgattt cgagaacggt ggtgtcggcg attgacgaca ccggctacgc
      961 ccgcaccgac aatatcgatg cggtggccga ttccgccggt cagctcatcg ttcatggcct
     1021 cgaccgcgct ttcggctggc agatcgtgat ccatcatacg agcccggccg cctcggtaag
     1081 cttcgcttcg gcggattcgg tcatcgccgg cttcggcacg tgcagccacg aatagggccg
     1141 tctgattgca ggcgatagcg cttcaacccc ggcccggctg acgggccggg gtttctcatg
     1201 cgtgccccgt gatggacggc tcgcggctgt ccttctcagt gtcgcgtttt gaggctaagc
     1261 ttgcgataag gcttggcgcg tgaatgtcag tcggggggca gcaggtgcgt catcggtgtg
     1321 gtctctcgca gcggcggctc gcgcttgtcg cgttcgccgc cgccatatcg ctcataggcg
     1381 ctaacgcggc gtgggccggc ccttacgagc aatcgccgag cttcacggcc acgcaggtgc
     1441 tctcgccaaa tctgctcaac agccccaact acaccatcgg caatcaagtc ggcctcgaca
     1501 atttccagta tgttttcgac gtcgacacga gatggggcac ctattcggtc aagggctccg
     1561 atctgatgcg ggtcagagcc cgcgagatcg cggcgaccgc ggcgctcgaa cggatcgaca
     1621 gcgccggcac gctcgtcaac gcggcgggac gcacggcgct caagccgctc gctaccgcaa
     1681 aggatctcgt cacggctccc ggcaggacca tcggcgatac cgtgcgcggc gttggcaacc
     1741 tgttcggcag cgtggatgcc gccgtgggcg cgaccgatcc gcacaaggag cgggtgatcg
     1801 cctcggtgac agggggcgcg accgcgcggc gcaagctcgc ctacgatttc ggcgtcgatc
     1861 ccaataccag ctttccgcct ctccacgacg agttgacgcg gctcgccacc gccaacgcca
     1921 tcggcgagac tagcaccaat gccgggctcg ccttcgtcac cggcggcgcc ggcatcgcca
     1981 tcagcgtcgg cggtacgagc cggacgctca ggcttgcgct ccgcgacaag acggcggctc
     2041 aactcgagaa ggaggggcgc cagttgctcg caacgatggg cgtgccggcc gccaccacga
     2101 acgccttcta tgccaatccg catctctcac cgaccgacaa ggcgatcatc gtcgcggcgc
     2161 tcatgaatct cggcgcgacg gccgggcgcg agatcttcat cgccagcgct gccgacacgc
     2221 agagtatcga gatgggcttc ttctatcgcc gccaggccga gcttatcgcg agcttcgata
     2281 agaaggtcgc gccggtcacg gccttcgtgc gagttggcgg cgcgccgatg ctgcaaaccg
     2341 gcaagggcac ggttagcatc ctgcccgtcg actatctcta ttggtcgccg ccgctggagg
     2401 gcctgatcgc gggcgccgga ggcggcggtc agatgtggat cacgggacgg gcgagcgaga
     2461 gagcgagggc gcaacttgcc gcgcgcggct ggacgctcgt gcccaaggcc ggcggcaagc
     2521 tcgcggatta ggtcagaggc gcaaccggcg accccggccg gagcacaaga ggcgccggac
     2581 gaccctcacg aacttgcttc agagggcctc tcggactgcc gtggccgcca cgacgcgcgt
     2641 agcgatttcg caccgagaat ggcgaggtat acagc
//
LOCUS       DASUQP010000053         5887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1660005, whole genome shotgun sequence.
ACCESSION   DASUQP010000053 DASUQP010000000
VERSION     DASUQP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660005"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /gene="hslV"
                     /locus_tag="VFY74_02380"
     CDS             complement(<1..505)
                     /gene="hslV"
                     /locus_tag="VFY74_02380"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX5930587.1"
                     /translation="MPVPMGPSPSFSETWHGTTILTVRKGGKVVIAGDGQVSLGQTII
                     KGTAKKVRRVGSGNVIAGFAGSTADAFTLFERLETKLDRFPDQLKRACVELAKDWRTD
                     RYLRRLEAMMLVADADESLVLTGTGDVLEPDDAVMGIGSGGPYALAAARALLDGPLDA
                     EQIARKAM"
     gene            637..1269
                     /gene="hisB"
                     /locus_tag="VFY74_02385"
     CDS             637..1269
                     /gene="hisB"
                     /locus_tag="VFY74_02385"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX5930588.1"
                     /translation="MLKRSKDTSPRRTTTARRASVTRKTKETVISASVDLDGTGAYEI
                     ATGVGFLDHMIEQLARHSLIDITLKAEGDLHIDQHHTTEDAGIVLGQAVAKALGDKKG
                     IARYGFAYLSMDETLTRVALDVSGRPFVMWRVAFTRSKIGEMDTELFREWFQAFAQHA
                     GLTLHVETLYGDNSHHIAETCYKALARALRQAIAIDERQGGRVPSTKGQL"
     gene            1313..1816
                     /locus_tag="VFY74_02390"
     CDS             1313..1816
                     /locus_tag="VFY74_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEX5930589.1"
                     /translation="MTVYSVYEPPVPATDVIERADRIAFVKEGFSWPALFVPLLWVIY
                     HRMWIEFVVLLLVYIGLQLAFGTDAQGQTLTAFASFAIGLLFAFEANDLRTASLERRG
                     YRFAGVASGRDRIDAERSFFVTWLPQQERAAREAVTTPQPRRDPGARTPLRRAEGEEV
                     IGLFPRP"
     gene            1823..2473
                     /gene="hisH"
                     /locus_tag="VFY74_02395"
     CDS             1823..2473
                     /gene="hisH"
                     /locus_tag="VFY74_02395"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5930590.1"
                     /translation="MHVAIIDYGSGNLHSAAKAFERAAREHAPALSVRVTSKPEEVRA
                     AERIVLPGVGAFADCREGLARIPGMIETLEESVRRRGKPFFGICVGLQLMAERGLEHG
                     LTPGLGWIEGEVRAIAPNDRALKIPHMGWNTLALRRTHALFEGIATGDTGLNAYFVHS
                     YHFVPAGADALLATTDYGGPITAFVARDNLAGSQFHPEKSQKLGLALIANFLKWRP"
     gene            2625..3362
                     /gene="hisA"
                     /locus_tag="VFY74_02400"
     CDS             2625..3362
                     /gene="hisA"
                     /locus_tag="VFY74_02400"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX5930591.1"
                     /translation="MILFPAIDLKDGHCVRLRQGEMMQATVFNTDPGAQARTFEEQGF
                     QWLHVVDLNGAFEGRPVNEKAVEAIFEAVHISIQLGGGIRNLDTIVTWLDKGVDRVVL
                     GTAAVRDPALVREAARDFPGCIAVAIDARDGKVAIEGWSETSEMSALDLARRVEDAGV
                     AAIIHTDITRDGVLEGLNLDSSAAIARAVAIPVIASGGLAGIDDVKRLLEPRYAMLEG
                     AIAGRALYDGSLDPREALALLGEQHPF"
     gene            3365..4141
                     /gene="hisF"
                     /locus_tag="VFY74_02405"
     CDS             3365..4141
                     /gene="hisF"
                     /locus_tag="VFY74_02405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX5930592.1"
                     /translation="MLKIRLIPCLDVKEGRVVKGVKFVDLRDAGDPVEAAAAYDEAGA
                     DELCFLDITASHEKRGTILDVVRCTAERCFMPLTVGGGVRSIEDVRALLEAGADKVSI
                     NTAAVKDRNLVRAAAEKFGTQCIVVAIDAKRVRADGDRARFEIFTHGGREATGIEAVG
                     YAREVTGLGAGEILLTSMDRDGTRKGFDIELTRAIADAVPVPVIASGGVGTLDHLVEG
                     VLQGHASAVLAASIFHYGEHSIGEAKNHMAAAGLPMRLDP"
     gene            4242..4568
                     /locus_tag="VFY74_02410"
     CDS             4242..4568
                     /locus_tag="VFY74_02410"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX5930593.1"
                     /translation="MSKDVLSRLAATIKSRRLARPEDSHTRRLLDEAPIKPAKKLGEE
                     AVEVAIAALAQDKKALVGESADLLYHLLVVLESREVDLDDVFDELERRMALTAVGEKR
                     QKTASR"
     gene            4584..5537
                     /gene="coaA"
                     /locus_tag="VFY74_02415"
     CDS             4584..5537
                     /gene="coaA"
                     /locus_tag="VFY74_02415"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX5930594.1"
                     /translation="MNPRPQVAFSPYALYMREEWAGLRADTPMPLTEAELENLSGLTE
                     RISTTEVVEIYLPLSRLLNFYVEAAQRLHGATEKFLRKGNSKMPFIIGLGGSVAAGKS
                     TTARVLRALLARWPSHRRVDLVPTDGFLFPNAVLEERAMMDRKGFPESYDLPKLLNFL
                     AEVKSGQAPAEAPVYSHLVYDVLPDQKLIVERPDIVVVEGLNVLQPAKLPKDGEAIPY
                     VSDFFDFSIYIDAAEELVESWYVERFLRLRQTSFRDPASYFHRYASLSEEEARKTALS
                     IWRTINLVNLTEHILPTRQRADLILRKGADHAVETVALRKL"
     gene            complement(5690..>5887)
                     /locus_tag="VFY74_02420"
     CDS             complement(5690..>5887)
                     /locus_tag="VFY74_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5930595.1"
                     /translation="VARVVDLTGAGDLYSAGFLYGLTQGAPLAVCGRLGSLAAAEVIG
                     HIGARPEVSLRSLANEKGLIG"
BASE COUNT         1009 a         1945 c         1922 g         1011 t
ORIGIN      
        1 ccatggcctt gcgggcgatc tgttcggcgt cgagcggacc gtcgagcaga gcacgcgcgg
       61 ccgcaagcgc atagggaccg ccggagccga tccccatcac cgcatcgtcc ggttccaata
      121 cgtcgccggt gccggtcaag acgagactct cgtcggcgtc ggccacgagc atcatggcct
      181 cgagccggcg caaatagcgg tcggtgcgcc agtccttggc gagctcgaca caggcccgct
      241 tgagttggtc gggaaagcgg tcgagcttgg tttcgaggcg ctcgaacagg gtgaaggcgt
      301 cggcggtcga gccggcgaaa cccgcgatca cgttgcccga gccgacccgg cgcaccttct
      361 ttgccgtgcc cttgatgatg gtctggccga ggctgacctg gccgtcgccc gcgatcacga
      421 ccttgccgcc tttgcggacg gtcagaatcg tggtgccgtg ccaagtctcc gagaaagacg
      481 gggagggacc catgggtaca ggcatatcgg taggggcgca cccttgttca agctgcggcc
      541 cgttaacgct ctgatagcac tcttcctgcc cctcgcgtat aagcgagccg tgctcggaag
      601 cgaattgcta agagccttga ggttaagctc atcgccatgc tgaagcgaag caaggacacg
      661 agcccccgcc ggacaaccac ggcgcgccgg gcaagcgtga cccgcaagac caaggagacg
      721 gtgatatcgg cttccgtcga tctcgacggc accggcgcct atgagatcgc gaccggcgtc
      781 ggtttcctcg accacatgat cgagcagctt gcccgccatt cgctgatcga cattacgctg
      841 aaggcagaag gcgatctcca tatcgaccag caccacacga ccgaggatgc cggcatcgtg
      901 ctcggccagg ccgtggccaa ggcgctcggc gacaagaagg gcatcgcgcg ctacggcttc
      961 gcctatctct ccatggacga gacgctgacc cgtgtcgcgc tcgacgtgtc ggggcggccg
     1021 ttcgtcatgt ggagagtggc gttcacgcgg tcgaagatcg gggagatgga taccgagctg
     1081 ttccgcgaat ggttccaggc cttcgcccag catgccgggc tcacgctcca tgtcgagacg
     1141 ctctatggcg acaatagcca ccacatcgcc gagacctgct ataaggcgct cgcccgggcg
     1201 ctccgtcagg cgatcgccat cgacgagcgg caagggggcc gcgtgccctc gaccaagggc
     1261 cagctttgat cgattgccgg acttgccgcc ggcgcaacag ttgcgagtgg ccgtgaccgt
     1321 ctattccgtt tatgagccgc ccgttcccgc gacggacgtg atcgagcgcg cggaccggat
     1381 cgccttcgtc aaggagggct tctcctggcc cgcgcttttc gtgcccttgc tgtgggtgat
     1441 ctatcatcgc atgtggatcg agttcgtcgt gctcctcctc gtctatatcg ggctgcaact
     1501 cgccttcggt accgacgcgc aagggcagac gctgacggcc tttgcaagct tcgccatcgg
     1561 tctcctgttc gccttcgagg cgaacgattt gcgcacggct tcgctggagc ggcgcggcta
     1621 tcgctttgcc ggcgtggcca gcggccgcga ccgcatcgac gccgagcgct cattcttcgt
     1681 tacctggctg ccgcaacagg aaagggccgc gcgcgaagcc gtcacgacgc cgcagccgcg
     1741 gcgcgacccc ggagcgcgga cgccgcttcg gcgcgcggag ggcgaggagg tcatcgggct
     1801 cttcccgcgg ccttgaaccg ccatgcacgt cgccatcatc gattacggtt cggggaacct
     1861 ccactccgcc gccaaggcct tcgagcgggc ggcgcgggaa cacgcccctg cgctttccgt
     1921 cagggtgacg tcaaagcccg aggaggtgcg cgcggccgag cgcatcgtcc tgccgggcgt
     1981 cggcgccttc gccgattgcc gggagggcct tgcccgcatt cccggcatga tcgagaccct
     2041 cgaagagtcc gtgcgccggc gcggcaagcc cttctttgga atctgcgtcg ggcttcagct
     2101 catggccgag cgcgggcttg aacatggtct cacgccgggg cttgggtgga tcgaaggcga
     2161 ggtgagggca attgcgccca acgatcgcgc gctcaagatt ccgcatatgg gctggaacac
     2221 gctcgcgctg cggcggacgc atgccctgtt cgagggcatt gcgacgggcg acaccgggct
     2281 caatgcctat ttcgtccact cctaccattt cgtgccggcc ggggccgacg cgctcctggc
     2341 caccaccgac tatggcggtc ccatcaccgc cttcgtcgcc agggacaatc tcgcgggcag
     2401 ccagttccac cccgagaaga gccagaagct cgggctcgcg ctgatcgcca acttcctcaa
     2461 gtggcgcccg tgagcctccc ggtgatgttc acactccctc cccctggaag ggggagggcc
     2521 ggggtggggg tttctcgctc ccgaacctcg acccccaccc gacgttcccg gcttgacgcc
     2581 gggaccgtca ccctccccct ttctggggga gggaggacct agatatgatc ctgttcccgg
     2641 ctatagactt gaaggacgga cactgcgtgc gtctccgcca gggcgagatg atgcaagcca
     2701 ccgtgttcaa caccgatccc ggggcgcagg cgcggacctt cgaggaacag ggttttcagt
     2761 ggctgcacgt ggtcgatctg aacggagcgt tcgagggccg gccggtgaac gagaaggctg
     2821 tggaggcgat cttcgaggcc gtgcacatct cgatccagct cggcggcggc atccgcaacc
     2881 tcgacaccat cgtgacgtgg ctcgacaagg gcgtcgatcg cgtcgttctc ggcacggcgg
     2941 cggtgcgcga tcccgccctc gtgcgcgaag ctgcgcgcga tttcccgggc tgcattgccg
     3001 tcgccatcga cgcgcgcgac ggcaaggtcg cgatcgaagg ttggtccgaa acgagcgaga
     3061 tgagcgcgct cgatcttgcc cgcagagtcg aagacgccgg cgtcgccgcg atcattcaca
     3121 ccgacatcac gcgcgacggc gtgcttgagg gactgaacct cgactcgtcg gccgcgatcg
     3181 cgcgcgccgt cgccattccc gttatcgcga gcggcggcct tgccgggatc gacgacgtga
     3241 agaggctgct cgagccgcgc tacgcgatgc tcgaaggcgc gatcgccggg cgcgcgctct
     3301 acgacggaag tctcgatccg cgcgaggcgc ttgcgttgct cggtgagcag cacccgttct
     3361 gatcatgttg aagatccgcc ttataccctg cctcgacgtg aaagaggggc gcgtcgtcaa
     3421 gggcgtcaaa ttcgtcgatc tgcgcgatgc cggcgatccg gtggaagccg ccgccgctta
     3481 cgacgaggcg ggcgccgacg agctctgctt cctcgacatc acggcgagcc atgagaagcg
     3541 cggcaccatt ctcgacgtgg tgcgctgcac ggcagaacgc tgcttcatgc cgctcaccgt
     3601 cggcgggggc gtgcgctcga tcgaagacgt ccgtgcgctc ctcgaagcgg gcgccgacaa
     3661 agtctccatc aacacggcgg cggtgaagga ccgcaacctg gttcgcgcgg cggccgaaaa
     3721 attcgggacg caatgcatcg tcgtcgccat cgacgccaag cgcgtccgag ccgacggcga
     3781 tagggcgcgg ttcgagattt tcacccatgg cgggcgcgag gcgaccggca tcgaagcggt
     3841 cggctacgcg cgcgaggtga ccgggctcgg ggcgggcgaa atcctgctca cctccatgga
     3901 ccgcgacggc acgcgcaaag gcttcgacat cgaactcacc cgggctatcg ccgatgccgt
     3961 cccggtgccg gtgatcgcga gcggaggcgt gggcacgctc gatcacctgg ttgagggcgt
     4021 cctccagggc cacgcctcgg ccgtgctggc cgcttcgatt ttccactacg gcgaacactc
     4081 gatcggcgag gcaaagaacc acatggcggc cgcaggcctg cccatgcggc tcgatccata
     4141 aacttcgatc gagcctgaac gcccggcaat gcctcttttt ggtcaggatt acccggacgt
     4201 ccctattttc atgatcgctt cgtgggtgta tgagccaagt catgagcaaa gacgtcttga
     4261 gtcgccttgc cgccacgatc aagtcgcgcc ggttggcgcg ccccgaggac tcccatacgc
     4321 gtcggcttct cgacgaggcg ccgatcaagc cggccaagaa gctcggcgag gaggccgtcg
     4381 aggtcgccat cgcggcgctc gcccaggaca agaaggccct ggtgggcgag agcgccgacc
     4441 tgctctacca tctgctcgtg gttctggagt cgcgcgaggt cgatctcgat gacgtgttcg
     4501 acgagctgga gcggcgcatg gcgcttacgg cggtcggcga gaagaggcaa aagaccgcgt
     4561 cgcgctgacg gatgagcgtt tccatgaatc cgcgtcccca agtcgcgttc tcgccctacg
     4621 ccctctatat gcgcgaggag tgggcagggc ttcgggccga cacgcccatg ccgctcaccg
     4681 aggccgagct ggagaatctc agcggcctca cggagcgcat ctccaccacg gaggtcgtgg
     4741 aaatctacct gccgctcagc cggcttctca atttctatgt cgaggccgcg caaaggctgc
     4801 acggcgccac ggaaaagttc ctgcgcaaag gcaacagcaa gatgccgttc atcatcgggc
     4861 ttggcggaag cgtcgccgcg ggcaagagca cgacggcgcg cgttcttcgg gccttgcttg
     4921 cgcgctggcc gagccatcgg cgggtcgatc tcgtgccgac cgacgggttc ctcttcccca
     4981 atgccgtgct cgaagagcgc gcgatgatgg accgcaaagg ctttcccgag agctacgacc
     5041 tgcccaagct gctcaacttt ctcgccgagg tgaagtcggg acaggcgccc gccgaggcgc
     5101 cggtctactc gcacctcgtc tatgacgtgc ttcccgatca gaagctcatc gtcgagcgac
     5161 cggacatcgt cgtcgtcgaa ggcctcaacg tgttgcagcc cgccaagctg ccgaaggacg
     5221 gagaggccat cccgtatgtt tccgactttt tcgacttttc gatctacatc gacgcggccg
     5281 aggagctggt ggagagctgg tacgtcgagc ggttcttacg cttgcggcaa acctcgttcc
     5341 gtgaccccgc ttcctacttc caccgctatg cgtctctgtc ggaggaggag gcgcgcaaga
     5401 ctgcgctctc gatctggcgc acgatcaatc tcgtcaatct gacggagcat atcctgccta
     5461 cgcgccagcg cgccgatctc atcctgcgca agggcgccga ccacgcggtc gaaacggtgg
     5521 cgctcaggaa actttgatcg cgtctcgcct tgaatgacgg cgaggattac ttcggcggcg
     5581 ggtagagcgc ggcaggcccg tcgtaaatgc cgccgacacc tttcaccgcg gagccgacaa
     5641 acgagaaggc gatttcgcgc ctgcgtggcc gaaacaccac agccgagggt tagcctatga
     5701 ggcccttctc gttggcgagc gatctcaagg aaacctcggg ccgggcgccg atatggccga
     5761 tcacctcggc ggcggcgagg ctccccaggc ggccgcaaac ggccagtggc gccccttgcg
     5821 tcagcccgta gagaaagccg gcggagtaga ggtctccggc cccggtcaaa tcgaccacgc
     5881 gagcgac
//
LOCUS       DASUQP010000054         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1252739, whole genome shotgun sequence.
ACCESSION   DASUQP010000054 DASUQP010000000
VERSION     DASUQP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252739"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFY74_02425"
     CDS             complement(<1..245)
                     /locus_tag="VFY74_02425"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX5930596.1"
                     /translation="MSFTGVNYVAVIIATLAGFGLGTVWYMVLSKPWMRAVGKTEMDR
                     PSGPAQFLPFVIAIVALFVMALMLAGLMGHIGDITVRG"
     gene            complement(311..742)
                     /locus_tag="VFY74_02430"
     CDS             complement(311..742)
                     /locus_tag="VFY74_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX5930597.1"
                     /translation="MAYWLLKSEPHAWSWDQQKQEGRKGAEWDGVRNFQARNNMRAMR
                     QGDLAFFYHSGEEKSVVGIVKVVKEAHPDSTDGTGQWECVDVAAVRDLARPVTLAAIK
                     AEPSLKSMVLVRNSRLSVQPVTAAEWKRICAMGGVKDPPRA"
     gene            complement(764..1060)
                     /locus_tag="VFY74_02435"
     CDS             complement(764..1060)
                     /locus_tag="VFY74_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5930598.1"
                     /translation="MLYALICTDKPGSLAVRKANRPQHLAYLEGLGDALVFAGPFTEQ
                     DGQTMNGSLIVIEASSLDDARNIAAGDPFAKLGIFANVDIRPWLWAVKKPDKEK"
     gene            complement(1108..2106)
                     /locus_tag="VFY74_02440"
     CDS             complement(1108..2106)
                     /locus_tag="VFY74_02440"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5930599.1"
                     /translation="MALQSVGIVGAGAWGTALAVTARRAGRDVLIWAYEPETLADLAR
                     NHRNELYLPGVRLDPAIRATDRLREIADCDFLLLTTPAQHAREIVGGLAPHLKQAQPL
                     VNCSKGIEQATGNLISQVVAEVAPGAKIAVLSGPSFAAEVARGLPAAVTLATAEETLG
                     RALAHALGHPPFRCYWSDDVVGAEIGGAVKNVYAIAAGIVAGKRLGASAHAALVTRGF
                     AEMARFGTALGARRETLTGLSGLGDLVLTCGSAQSRNMSLGMELGKGRRLDEVLEERL
                     GVTEGVYTAGALVQMAAARGIDMPIAQAVHAVISGLATVDEAIEALLARPLRAEVE"
     gene            complement(2173..3261)
                     /gene="tsaD"
                     /locus_tag="VFY74_02445"
     CDS             complement(2173..3261)
                     /gene="tsaD"
                     /locus_tag="VFY74_02445"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5930600.1"
                     /translation="MTAKDASLTVLGIETSCDETAAAIVRRGAGGRGEILASVVRSQL
                     DLHAAYGGVVPEIAARAHVELLDAAIRQAMGEAGLAYDAIDAVAAAAGPGLVGGLIVG
                     VTTAKAIALGLGRPFIAVNHLEAHALTAGLTDGLDFPYLLLLVSGGHTQLLIVKGVGD
                     YERLGTTLDDALGEAFDKAAKLLGLGYPGGPEVEREAAKGRASIALPRPMLGREEPHF
                     SLAGLKTALRHEALARAPLSGQDVADLCASFQEAVADIVSDRTARALHLYRARLGNGA
                     QRMLVVAGGVAANRRLKAALETVAGARRFRLVVPPPELCSDNAAMIAWAGAMRLSRGL
                     TDDLSAPVRARWPLDPDAPPAKGAGVKA"
     gene            3387..>3751
                     /locus_tag="VFY74_02450"
     CDS             3387..>3751
                     /locus_tag="VFY74_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5930601.1"
                     /translation="MPSRPLLIGTRGSPLALWQARHVEARLLAAHGFAEEAIGLKVIT
                     TSGDRIHDLPLRDFGGKGLFTKEIDEALLSGAVDLAVHSMKDLPTDLPPGIVIAAVLK
                     RGDVRDAFISASGETLAALP"
BASE COUNT          613 a         1293 c         1232 g          613 t
ORIGIN      
        1 cctctcaccg tgatgtcgcc gatatgcccc atgagcccgg cgagcatgag cgccatgacg
       61 aacagggcga cgatggcgat cacgaacggc aagaactgcg ccgggccact cggccggtcc
      121 atctcggtct tgccgacggc gcgcatccag ggcttcgaca ggaccatgta ccacaccgtt
      181 ccaaggccga agccggccag cgtcgcgatg atgactgcga cataattcac gcctgtgaac
      241 gacatgagct ttcccctccc tggttcgagc cgctgtctcc gactttacct caggaaggcg
      301 gctcacggca ctaagcgcgc ggcggatcct tgaccccgcc catggcgcag atcctcttcc
      361 actcggccgc cgtcacaggc tgtaccgaca ggcgcgaatt tcgtaccagg accatggact
      421 tgagcgaagg ctcagccttg atcgcggcaa gcgtcacggg acgggcgagg tcccttacgg
      481 ccgccacgtc gacgcattcc cattggccgg ttccgtcggt cgagtcgggg tgggcttcct
      541 tcaccacctt gacgatgccg acgacgctct tctcctcgcc ggaatgatag aagaaggcga
      601 ggtcgccctg ccgcatcgcc cgcatattgt tgcgcgcctg gaaattgcgc acgccgtccc
      661 actcggcgcc cttgcgccct tcttgtttct gctggtccca ggaccaggcg tgaggttcgg
      721 atttgagcag ccaataggcc atgcgagcga agcggcgcga ggctcacttc tccttgtcgg
      781 gctttttcac ggcccacagc cagggcctga tatcgacgtt ggcgaagatc ccgagtttgg
      841 caaaaggatc gcctgcggcg atgttccgcg cgtcatcgag cgacgatgcc tcgatgacga
      901 tcaggctgcc attcatggtt tggccgtcct gctcggtgaa gggtccggca aagacgagcg
      961 cgtcgccgag cccctcgaga taagcgagat gctgcgggcg atttgccttg cggacggcaa
     1021 ggctgcccgg cttgtcggtg cagatcagcg cgtacagcat cgagcctctc tcgccttcag
     1081 gctaatgcgc gcctgatttt ctgtccatca ttccacctcg gccctgagcg gccgggcgag
     1141 cagcgcctcg atcgcctcat cgaccgtggc gagtcccgag atcaccgcgt gcacggcctg
     1201 cgcgatcggc atgtcgatgc cgcgcgccgc cgccatctgc acgagagcgc ccgccgtgta
     1261 gacgccctcg gttacgccga gacgctcctc aagcacttca tcgagcctgc ggccttttcc
     1321 aagctccatc ccaagcgaca tgttgcgcga ttgcgcgctc ccgcaggtga gcacgagatc
     1381 gccgaggcca gacaagccgg tgagcgtctc gcggcgggca ccaagcgccg tgccgaatcg
     1441 cgccatctcg gcaaagccgc gggtcacgag cgcggcatgg gcgctcgcgc cgagtctctt
     1501 gcctgcgacg atgcctgcgg caatggcata gacgttcttg accgctcccc cgatctcggc
     1561 gcccaccacg tcatcgctcc aatagcaacg gaacggcgga tgaccgagcg cgtgggcgag
     1621 cgcgcgccca agcgtctctt cggcggtggc aagcgtgacg gctgccggca gcccgcgcgc
     1681 gacctctgcc gcgaaactcg gccccgacag cacggcgatc ttggctccgg gcgcgacctc
     1741 cgccacgact tgcgagatca ggttgccggt cgcctgctcg atgcccttcg agcagttcac
     1801 gagcggctgc gcttgcttga ggtgaggggc gaggccgccc acgatctcgc gcgcgtgttg
     1861 ggcgggcgtc gtgagcagca ggaaatcgca atcggcgatt tcgcgtaagc ggtcggtggc
     1921 acggatcgcg gggtcgaggc gcacgccggg aagatagagt tcgttgcggt ggttccgggc
     1981 gagatcggcg agcgtttcgg gttcataggc ccagataaga acgtcgcggc cggcgcgacg
     2041 ggctgtgacg gcgagcgccg tgccccacgc gcccgcgccc acgatgccga ccgattgaag
     2101 cgccatgccg agacctcgcc gcgttcagcc ttcatatgcc agattttgcg tgagccacga
     2161 aatcggcgcg ccttaagcct taacgcctgc gcctttggcc gggggcgcgt cggggtcgag
     2221 cggccagcgg gcacggacgg gcgccgagag atcgtcggtg agcccgcgcg agagccgcat
     2281 cgcgcctgcc caggcgatca tggcggcatt gtctgagcaa agctcgggcg gcggcacgac
     2341 gaggcggaag cgtcgcgcgc cagcgaccgt ttcgagcgcc gccttcagcc tccggtttgc
     2401 ggcgaccccg cccgcgacga cgagcatgcg ctgagccccg ttgccgaggc gcgcccggta
     2461 aagatggagc gcgcgcgcgg tcctgtcgct gacgatgtcg gcaaccgcct cctggaagct
     2521 cgcgcagaga tcggcgacgt cttggccgct gagtggcgcc cgcgccagcg cctcgtgcct
     2581 gagcgcggtc tttagtccgg cgagcgagaa atgcggctcc tcccgcccaa gcatcgggcg
     2641 ggggagcgcg atgctcgcgc gtccctttgc cgcttcgcgc tcgacttccg gccccccggg
     2701 atagcccagg cccaggagct tggcggcctt gtcgaaggcc tcgccgagcg catcgtcgag
     2761 cgtggtgccg agccgctcgt agtcgccgac gcccttgacg atgaggagct gggtatgccc
     2821 gcccgacacg agcaggagca ggtagggaaa gtcgagcccg tccgtcagcc ccgcggtgag
     2881 cgcgtgggcc tcgagatggt tgacggcgat gaagggacgg ccgagaccga gcgcgatcgc
     2941 cttcgccgtc gtcacgccca cgatcaaccc gccgacaagg ccaggtcccg ccgccgcggc
     3001 aaccgcatcg atcgcgtcat aagcgagccc ggcttcgccc atcgcctggc ggatcgccgc
     3061 atcgagcagc tccacatggg cgcgcgcggc gatctccggc acgaccccgc cataagcggc
     3121 atgcaggtcg agctgcgagc gcaccacgct tgcaaggatc tcgccgcgcc cgcccgcccc
     3181 gcggcgcacg attgcggcag cggtctcgtc gcagctcgtc tcgatcccga gaacggtcag
     3241 ggacgcgtct ttggcggtca tgtccatggc gctttctata gcctcaaagg acctcgattg
     3301 aaggggaagc ggaaagaggc cattaaaggg gggccaagga tgcggccgaa acggccgcct
     3361 cttaaggcaa ttcaaggagt tcacccctgc cttcccgccc cctcctgatc ggcacccgcg
     3421 gcagcccgct tgcgctttgg caggcacggc acgtcgaggc gcggctcctc gccgcccacg
     3481 gcttcgccga agaggcgatc gggctcaagg tcatcaccac ctcgggcgac cgcattcacg
     3541 acctgccctt gcgcgacttc ggcggcaagg ggctgttcac caaggagatt gacgaagcat
     3601 tgctcagcgg cgcagtcgat ctcgccgtcc attcgatgaa ggatttgccc actgacctgc
     3661 cacccggcat cgtcatcgcc gccgtgctga agcgcggcga cgtgcgcgac gccttcatca
     3721 gcgcgagcgg cgagacgctt gcggcgcttc c
//
LOCUS       DASUQP010000055         5394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_26034,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000055 DASUQP010000000
VERSION     DASUQP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26034"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFY74_02455"
     CDS             complement(<1..619)
                     /locus_tag="VFY74_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5930602.1"
                     /translation="MTVAETRAGREARRPLELVPLLAEAAEAAAGLRGRAVEGVAAKV
                     TAGGKIDAEALEREQHAAHGLAWIATYVEALQQLANYAKRLDGEGRFGEIERLLAEIG
                     AAEYLSQLAGGVAMSQGETARLHELGVGESDQASFLTPSVRALIAAATPEARARAVAL
                     IKNSLGAATYGDPGLDDTLQAIRDEMRRFSEAEVVPHAHEWHLKAE"
     gene            complement(714..2021)
                     /gene="ccrA"
                     /locus_tag="VFY74_02460"
     CDS             complement(714..2021)
                     /gene="ccrA"
                     /locus_tag="VFY74_02460"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX5930603.1"
                     /translation="MNEMSLAAVSQPKTAEAVKDLYEIGEIPPLGHVPKNMYAWVIRR
                     DREGPPETAMQVEMVATWELDSHDVLVLVMAAGVNYNGVWASLGTPVSMFDVHKQPYH
                     IAGSDASGIVWAVGSKVTRWKVGDEVVIHCNQDDGDDEECNGGDPMFSPSQRIWGYET
                     PDGSFAQFCRVQDRQLLERPKHLTWEEAACYTLCLATSYRMLFGHRPHTLKPGHNVLV
                     WGASGGLGSMAVQLCATAGANAIGVVSDEDKREFVMSLGAKGVINRKDFNCWGQLPKV
                     GTPEFDAWLKEARKFGKAIWEITGKGVNVDCVFEHPGESTFPVSCLVVKRGGMVVICA
                     GTTGYNLTMDARYVWMHQKRIQGSHFAHLLQASQANKLVVERRIDPCMSEVFPWEQIP
                     KAHMRMWRNEHRPGNMAVLVSAPCTGLRTFEDVLEASGQIHGG"
     gene            2365..4416
                     /locus_tag="VFY74_02465"
     CDS             2365..4416
                     /locus_tag="VFY74_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX5930604.1"
                     /translation="MSAKSSKRDKPWLVRTYAGHSTAKASNALYRKNLAKGQTGLSVA
                     FDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFEGIPLSEMNTSMTINATAAWL
                     LALYIALAEEQGAPKAKLTGTVQNDILKEYLSRGTYVFPPAPSLRLIKDVIVYTAREV
                     PKWNPTNVCSYHLQEAGATPVQELAFALATAEAVLDAVKDSGEVPAAEFPQVVGRMSF
                     FVNAGVRFITEVSKMRAFVDLWNEIAEKRYGVEDPALRRFRYGVQVNSLGLTEQQPEN
                     NVYRILLSMLAVTLSKKARARAVQLPAWNEALGLPRPWDQQWSLRLQQIVAYETDLLE
                     YGDIFDGSAEIDRKVEELKEQARAELARIQAMGGAVAAIDYMKEALIASNAARVEAIE
                     KGEQVLVGVNAYTETEPSPLAATGAEGILTVPESVEIEQVAALHAWRQRRDEKAVRTA
                     LVDLKGAAAEGRNIMPPSIAAAKAGVTTGEWGQVLREVFGEYRAPTGIHAKHPIDTAR
                     LEDVRRKVEEVSGRLGRTLTFVVGKPGLDGHSNGAEQIAVRATDVGMEVVYDGIRLTP
                     AEIVAQAREVRAHVVGLSILSGSHVSLVREVAQRLREAGLGDVPVVVGGIIPAEDVNV
                     LKQCGASAVYTPKDFQLNEIMAGIVELVEAKAAALVEQGVPVPPLRAREKVPLERRKP
                     A"
     gene            4527..4703
                     /locus_tag="VFY74_02470"
     CDS             4527..4703
                     /locus_tag="VFY74_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930605.1"
                     /translation="MNIHLSTPRRETFFVSVIIAVLVIIGLIVPLPFFTGYGFGLLLI
                     AYLILLAGVLLENL"
     gene            5030..>5394
                     /gene="bfr"
                     /locus_tag="VFY74_02475"
     CDS             5030..>5394
                     /gene="bfr"
                     /locus_tag="VFY74_02475"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX5930606.1"
                     /translation="MKGDPKVIEYLNKGLRSELTAINQYWLHYRLLDNWGLKDLAKYW
                     RKESIEEMQHADKFTVRIIFLEGFPNMQVLDPLHIGQNVKEVLDCDLKAEISARALYE
                     EAATHCHGVRDYVSRDLFE"
BASE COUNT          944 a         1751 c         1742 g          957 t
ORIGIN      
        1 actcggcctt caagtgccac tcgtgcgcgt gaggcacgac ttcggcttcg gagaagcggc
       61 gcatctcgtc gcggatggcc tggagcgtgt cgtcgagccc cgggtccccg taggtggcgg
      121 cgcccaggct gttcttgatc agcgcgaccg cgcgggcgcg cgcctctggc gttgccgccg
      181 caatcagggc gcggaccgaa ggcgtgagga acgacgcctg gtcgctctcg ccgacgccga
      241 gctcgtgcag ccgggcggtc tcgccttggc tcatggccac gccgcctgcg agttgagaga
      301 gatattcggc ggcgccgatc tcggcgagca gccgctcgat ctcgccgaac cggccctcac
      361 cgtcgagacg tttcgcatag ttcgcgagct gctgcagcgc ctcgacgtag gtcgcgatcc
      421 aggcgaggcc gtgggccgcg tgctgttcgc gctcgagcgc ctcggcgtcg atcttcccgc
      481 ccgccgtgac cttcgcggcg acgccttcca cggcacgacc gcgcaagccg gcggcggctt
      541 cggcggcctc ggcaaggaga ggaacaagct cgagaggccg ccgcgcctcc cggccggccc
      601 gtgtctcagc gacagtcacg atcaggctcc tgtcatcgaa ctttgcgcgt tctggagaac
      661 acggaagcct gctcagcctc gcgagtcaaa cctgaattgg cgtgccgctc gccctagccg
      721 ccgtggattt ggccgctcgc ttcgagcacg tcctcgaagg tccgtaagcc cgtgcagggc
      781 gcgctgacga gaacggccat gttgcccggc cggtgctcgt tccgccacat gcgcatatgc
      841 gccttgggga tctgctccca gggaaacacc tcgctcatgc aaggatcgat acgccgctcc
      901 acaacgagct tgttggcctg gctcgcctga agcagatggg cgaaatggga cccttggatg
      961 cgcttctgat gcatccacac atagcgggcg tccatggtga ggttgtagcc ggtcgtgccg
     1021 gcgcagatga cgaccatgcc gccacgcttc accaccaagc acgacacggg aaaagtcgac
     1081 tcgcccggat gctcgaagac gcaatcgacg ttgacaccct ttcccgtgat ttcccagatc
     1141 gctttgccga attttcgcgc ctctttcagc caggcatcga attcgggcgt gccgaccttc
     1201 ggcagctgac cccagcaatt gaagtccttg cggttgatca cgcccttggc gccgagcgac
     1261 atcacgaatt cgcgcttgtc ttcgtcggag acgacgccga tcgcgttggc gccggctgtg
     1321 gcgcaaagtt gcaccgccat ggagccgagg ccgcccgagg cgccccacac cagaacgttg
     1381 tgacccggct tcagcgtatg cggccgatgc ccgaacagca tgcggtagga cgtggcaaga
     1441 cagagcgtgt agcaggcggc ttcctcccac gtgagatgtt tcggacgctc gagcagctgg
     1501 cgatcctgca cccggcagaa ttgcgcgaag gagccgtccg gggtctcgta accccagatt
     1561 cgctgcgagg gcgagaacat gggatcgccg ccgttgcact cctcgtcgtc gccgtcgtcc
     1621 tgattgcagt ggatgacgac ctcgtcgccg accttccagc gagtgacctt ggaacccacc
     1681 gcccagacga tgccagaggc gtcggagccg gcgatgtgat agggctgctt gtggacgtcg
     1741 aacatggaaa ccggcgtgcc gagcgaggcc cagacgccgt tgtagttgac gcccgcggcc
     1801 atcaccagaa cgagcacatc gtgggaatcg agctcccagg tcgcaaccat ctcgacctgc
     1861 atggccgtct ctggcgggcc ttcccggtcg cgcctgatga cccaggcata catgttcttc
     1921 ggcacatggc cgaggggcgg gatttcgccg atctcgtaca gatccttgac cgcctcggca
     1981 gtcttaggct gagacactgc ggctagagac atctcgttca tccggttgtc ccccagacct
     2041 gcctggcggc gtgctattgc actgccgcaa ttgtgttcta ttcatctcgc gtctgcaaca
     2101 aatttgcgca gaagcatgcg caacggcgcc tcagggcgac ctctttcggg caagaatcct
     2161 taccttcggg ccttgaccgg gcagccccga ggaagcgcca tcaccccgtc gctctcccag
     2221 ggcaataagt cgcaacacgg ggccggctct tccgccgatt cggaaacacc ggcggtcgtt
     2281 gatggtttct tgggcaccgg tggctatgtt gctctcgggc ggcacccgtg cttcgatgcc
     2341 gaggccacgc gaccgagcag aatcatgagc gcaaaatcat ccaagcggga caagccctgg
     2401 ttggtccgca cctatgccgg ccattcgacg gccaaggcct cgaacgcgct ttaccggaag
     2461 aacctcgcca agggtcagac cgggctttcg gtcgccttcg acctgccgac ccagaccggc
     2521 tacgactccg accacccgct tgcccgcggc gaggtcggca aggtcggcgt gccgatctcc
     2581 catctcggcg atatgcggac cctgttcgag ggcatcccgc tcagcgagat gaacacctcg
     2641 atgacgatca acgccacggc cgcttggctg ctcgctctct atatcgcgct cgccgaggag
     2701 cagggcgcgc ccaaggccaa gctcaccggc accgtgcaga acgacatcct caaagagtac
     2761 ctgtcgcgtg gcacttatgt gttccccccg gccccgtcgc tcaggctgat caaggacgtg
     2821 atcgtctaca ccgcgcgcga ggtgccgaag tggaacccga ccaatgtctg ctcctaccat
     2881 ttgcaggagg caggcgcgac gccggtccag gaacttgcct tcgcgcttgc caccgcagag
     2941 gcggtgctcg acgcggtgaa ggattccggc gaagtgccgg cggccgagtt tccgcaagtc
     3001 gtcggacgca tgagcttctt cgtcaatgcg ggggtgcgct ttatcaccga agtttcgaaa
     3061 atgcgcgctt ttgtcgatct gtggaacgag atcgccgaga agcgttatgg ggtcgaggac
     3121 cccgcgcttc gccggttccg ctacggcgtg caggtgaact cgctcggcct gaccgagcag
     3181 cagcccgaga acaacgtcta tcgcatcctg ttgtcgatgc tcgcggtgac gctgtcgaag
     3241 aaggcgcgcg cgcgggcagt gcagctcccg gcttggaacg aggcgctcgg actgccccgg
     3301 ccttgggacc agcaatggtc gctcaggctg cagcagatcg tcgcctacga gaccgacctg
     3361 ctcgaatacg gcgacatctt cgacgggtct gcggagatcg accgcaaggt cgaggagctc
     3421 aaggaacaag cgcgtgccga gctcgccagg attcaggcga tgggcggcgc ggtcgccgcc
     3481 atcgactata tgaaagaagc gctgatcgcc tcgaacgcgg cgcgggtcga ggcgatcgag
     3541 aagggcgagc aggtgctggt cggggtcaac gcctataccg agaccgagcc gtcgccgctt
     3601 gctgcgaccg gcgccgaggg catcctcacc gtgccggaat cggtcgagat cgagcaggtc
     3661 gcggccctcc atgcctggcg gcagcggcgc gacgaaaaag ccgtccgcac cgcgctggtc
     3721 gacctcaaag gggcggcggc agagggccgc aacatcatgc cgccttcgat cgcggcggcg
     3781 aaggcgggcg ttaccaccgg cgaatggggc caggtcttgc gcgaagtgtt cggcgagtat
     3841 cgcgcgccga ccggcattca cgccaagcac ccgatcgaca cggcaaggct cgaagatgtc
     3901 cgccgaaaag tcgaggaggt ctcaggccgc cttggccgga cgttgacctt cgtcgtcggc
     3961 aagccggggc tcgacggcca ttccaacggc gccgagcaga tcgccgtgcg cgccaccgat
     4021 gtcggcatgg aggtcgtcta tgacggcatc aggctgacgc ctgccgagat cgtggcccag
     4081 gcgagggaag tgcgtgccca tgtggtcggg ctctccattc tgtcgggctc ccatgtctcg
     4141 ctggtgcgcg aggtggcgca gcgcctgcgc gaggcggggc tcggcgacgt tcccgtggtg
     4201 gtcggcggca tcatccccgc cgaggacgtc aacgtcctga agcagtgcgg cgcctcggcc
     4261 gtctacacgc cgaaggattt ccagctcaac gagatcatgg ccggcatcgt cgagctggtc
     4321 gaggcgaagg cggccgcgtt ggtcgagcag ggcgtgccag tgccgccatt gcgggcccgc
     4381 gaaaaagtcc cgctcgagcg gcgcaagccc gcttgatgct tggggcgtgg aacgatcggt
     4441 tgcgtggcgg gggcggcttt gcggctaaac ttgccgtgag tcgcgtctcg tttgcggcgt
     4501 ggctgcaaat ataaaagggg gacacaatga acattcactt gagcacgccg cggcgtgaga
     4561 cctttttcgt ttccgtcatc atcgccgtcc tggtcatcat cggtttgatc gtgccgttgc
     4621 cgttcttcac cggctacggc tttggcctgt tgctgatcgc ctatctgatc ctgctcgcgg
     4681 gcgtgctgct ggaaaatctc taggcgccgc agcatccccc gagagttcca ccagcctggg
     4741 ccgaggcttt tcgggcgcgc cgctgtaaac ggcgcggacg gcgcgctcgc actcgcgccc
     4801 ggctcaacca agcccgcggg gaaatggcga caagacggcc gatcatggct tagagagagg
     4861 catgatcgtc tgttcctgca ccatgatcac gtccgaagac attgccgaag cggtgtcggc
     4921 gctccgcagc caggatcctc tggtggtgct gacccccggc ctcctctacc gccagctcgg
     4981 caaaaggccc tcctgcggga gctgcctgcc gccgcgagga gtggttctga tgaaaggcga
     5041 tccgaaggtc attgaatatc tcaacaaggg cctgcgcagc gagctgaccg cgatcaacca
     5101 gtattggctg cattaccggc tgctcgacaa ttgggggctc aaggatcttg ccaagtattg
     5161 gcgcaaggaa tcgatcgagg agatgcagca cgccgacaag ttcacggtcc gcatcatctt
     5221 cctcgaaggc ttccccaaca tgcaggtgct cgatccgctc cacatcggcc agaacgtcaa
     5281 ggaagtcctc gactgcgatc tcaaggccga gatctccgcg cgtgcgctat acgaggaggc
     5341 ggcgacgcat tgccacggcg tgcgcgatta tgtgagccgc gatctgttcg agaa
//
LOCUS       DASUQP010000056         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_435679,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000056 DASUQP010000000
VERSION     DASUQP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435679"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFY74_02480"
     CDS             <1..881
                     /locus_tag="VFY74_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5930607.1"
                     /translation="DLILLDVMMPGMDGYEVCRRLKDNPATRDIPIIFLTAKTEEVDE
                     EKGFDVGAVDYIHKPFSAPIVLARVRTQLALQEALIEARDARNQADQLLHALLPKIAA
                     DEIRSIGTVIPRRYENVAVLFCDVTNFTAYCDQHEPEDVVSRLDALFVIFERIAAKHG
                     LEKIKTIGDGFMAAAGLLHKVDDPVGAAVRCGLEMASTLIDAHLGWEVRVGVHSGPVV
                     AGVVGQERYQFDIWGDTVNVAARMVAMSTPGSVAATKDIFEQFGSSFDGESLGELDVK
                     GKGTIAVVGLRTRSTH"
     gene            complement(913..1740)
                     /locus_tag="VFY74_02485"
     CDS             complement(913..1740)
                     /locus_tag="VFY74_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5930608.1"
                     /translation="MTLRRGTALRVMDLLLWLIGLYLAICALAYFGNRAFMYFPDPAR
                     IPPAETGLDGVEEIEFAAADGTKLVAWYAPAEDGKPTLLYFHGNAANAANRAPKIALM
                     LGSGFGVFYLNNRGYGGSEGKPTEANNVADAIAAYDDLGRRGVPAGKVVAYGESLGAG
                     QAVRVAAERPLAGVVLEAPLTSTVDVARPVYFWLPLSLLIADQYNTERHIRFVTAPVL
                     ILHGEEDEVIPVEMGKRLYRAANDPKRIETFPHGRHNDLFDHGAWETMRNFLISLPQ"
     gene            complement(1737..2192)
                     /locus_tag="VFY74_02490"
     CDS             complement(1737..2192)
                     /locus_tag="VFY74_02490"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5930609.1"
                     /translation="MTILVALHALAAVIWVGGMFFAYMVLRPSAGPLDSPVRLALWAR
                     VFGRFFPWVWASIAVLLVSGYAMLFLGFGGFAGAGLHIHIMQATGILMMLLFLHLFFA
                     PWRRFSRAVDAGTYPEAAPALNQIRRIVAINLVLGLVTVAAGASGRFWP"
     gene            complement(2686..3123)
                     /locus_tag="VFY74_02495"
     CDS             complement(2686..3123)
                     /locus_tag="VFY74_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930610.1"
                     /translation="MRISILFRAALVALIVLLGVGLKSANADSGNITLTIYKGGWIIG
                     GSAGGGTLTFRGRSYQLGVGGIDYGLLFGGSKTVLRGRVRNIQRASDVAGVYAAAGAG
                     LAVGGGVRAIVLTNQKGAVLELSGRQVGLMANADLSGLAITMK"
BASE COUNT          670 a         1088 c         1074 g          579 t
ORIGIN      
        1 ccgacctcat cctgctcgac gtaatgatgc ccggcatgga cggctatgag gtgtgccgga
       61 ggctcaagga caatccggcc acgcgcgaca ttcccattat tttcctcacc gccaagaccg
      121 aggaagtcga cgaggaaaag ggcttcgatg tcggcgccgt cgactacatt cacaagccgt
      181 tctcggcgcc gatcgtcctg gccagggtca ggacccagct cgcgcttcag gaggcattga
      241 tcgaggccag ggacgcgcgc aaccaggccg accagctgct gcacgcgctg ctgccgaaga
      301 tcgccgccga cgagatccgc agcatcggca cggtgattcc acggcgttac gagaatgtcg
      361 cggtcctgtt ctgcgacgtg acaaacttca ccgcctattg cgaccagcac gaacccgagg
      421 acgtggtgtc gcggctcgat gcgctcttcg tcattttcga gcgcatcgcg gcaaagcatg
      481 gactcgaaaa gatcaagacc atcggcgacg gctttatggc ggccgccgga ctgctccata
      541 aagttgacga tccggtcggt gccgcggttc gctgcgggct ggagatggcc tcgacgctga
      601 tcgatgccca tctcggctgg gaggtgaggg ttggcgtcca ttccgggccc gtcgtagccg
      661 gcgtcgtcgg tcaagagcgt tatcagttcg acatttgggg cgacaccgtc aatgtcgccg
      721 cccgcatggt cgccatgagc acgccgggaa gcgtcgccgc caccaaggac atcttcgaac
      781 aattcggctc atccttcgac ggcgaatcgc tcggcgagct cgacgtcaag ggcaaaggca
      841 ccatcgccgt cgtcggcctc cgcacccgca gcacgcatta gagcgcgcgc cggccagcgg
      901 gcgagacggt gcctactgcg gcaatgaaat gagaaagttt cgcatcgtct cccaggcgcc
      961 gtgatcgaac agatcgttat ggcggccgtg gggaaacgtt tcgatccgtt tcggatcgtt
     1021 ggcggcgcgg tataggcgct tgcccatctc gaccgggatc acctcgtcct cctcgccatg
     1081 caggatgaga acgggcgcgg tgacgaacct gatgtggcgt tcggtattgt attgatctgc
     1141 aatcaatagg ctgagcggca gccaaaaata gaccgggcgc gccacatcga cggtcgaggt
     1201 gagcggcgcc tccagcacca cgcctgcgag gggacgttcc gccgcgacgc gcaccgcctg
     1261 ccccgctccg agagactcgc cataggcgac aaccttgccg gcgggcacgc cgcgccgccc
     1321 gagatcgtca taggccgcaa ttgcgtccgc gacgttgttc gcctcggtcg gctttccctc
     1381 cgagccgcca taaccgcggt tgttgaggta gaacacgccg aacccggatc cgagcatcag
     1441 ggcgattttc ggtgctcgat tggcggcgtt ggccgcgttg ccgtggaagt aaaggagcgt
     1501 cggcttgccg tcctccgcgg gggcatacca ggcgacgagc ttggtgccgt cggcagccgc
     1561 aaactcgatc tcctcgacgc cgtcaaggcc ggtctcggcc ggagggatgc gcgccggatc
     1621 ggggaagtac ataaacgcgc gattgccgaa ataggccagc gcgcagatcg cgagatagag
     1681 gccaatcagc cagaggagga gatccatgac gcgcagcgca gtgcctctcc gcaatgtcac
     1741 ggccaaaagc gcccgcttgc cccggcggcg acggtgacga gaccgagcac gagattgatg
     1801 gcgacgatgc ggcggatctg attgagcgcg ggtgcggctt ccggatacgt ccccgcatcg
     1861 acggcgcggg agaaccggcg ccagggcgcg aagaaaagat gcaggaacaa gagcatcatc
     1921 aggatgccgg tcgcctgcat gatatggata tgcagcccgg cgccggcgaa gcctccgaag
     1981 ccgaggaaca gcatcgcgta gccgctgacc aggagaacgg cgatgctcgc ccaaacccaa
     2041 gggaagaagc gcccgaagac gcgggcccag agcgcaagcc taacgggcga atcgagcggc
     2101 cccgcggagg gacggaggac catataggcg aagaacatgc cgccgaccca aatcaccgcg
     2161 gcgagcgcat gcagcgcaac cagaattgtc atgcgccaag cctacaccag ttgcggcggc
     2221 agattgtcaa gcgcgcatga accgcccgcg aactgccaac gcgcgtcttg aggcgcgaat
     2281 aggcctctgc gatgcggctc ggtcgcgaat ctgcgcttgc ccaagccata ggaacctcgg
     2341 atcgccgtag cgcacgagcg ctttaccagt tgcctgcaac gcccttctgc ggcgtaccga
     2401 actgcattat tctcgacccc ggacgacttg gcagcaaggt gcgcaacatg agaattctga
     2461 ttgccgtgtt ggccgcaggc ctgacgctga ccttcgctaa gttcggtctc ggcacaagcg
     2521 aggcggtggc ggcgagcttt tgcaaacatc gctacagtct ctgcctcgcc cgttgcccgg
     2581 ggccgtttgc gaggtgctca agccgctgtc aggtgcgata tcgctcgtgc atctatccgg
     2641 cgccctccat tcgagacctc ctttagctgt tcgggggctg ccgggctatt tcatcgtgat
     2701 cgccagcccg ctcaggtcgg cgttcgccat caggcctact tgccggcccg agagctccag
     2761 aaccgcgccc ttctggttgg tgagtacgat cgcgcggaca ccgccgccga ccgcaagccc
     2821 cgcgccggcc gcggcataga cgccggcgac gtcggacgcg cgttggatgt tgcgcacccg
     2881 gcccctcagc accgtcttcg agccgccgaa cagcaggccg taatctatgc cgccgactcc
     2941 aagctggtag ctgcgcccgc ggaaggtcag cgtcccgccg ccggccgaac cgccaatgat
     3001 ccagccgccc ttgtagatcg taagagtgat gttgccgctg tcggcgttcg cgctcttaag
     3061 cccgacgcca agcaggacga tgagcgcgac cagagcggct cggaacagga tcgagattcg
     3121 catgcttgac tctccctcgt ttcagtggcc gcggcttcga cacggacgat attcctccgt
     3181 tccacctgag acgccgcgag gcgacttccc aatttcggcc gaggccgaag ttatatcaca
     3241 ctatctttta catcggtgct cgtgacatcc ccggagttta gtcgctcgac gttatgccaa
     3301 aaacagactt tagcagccgc tataatgcag ccgtcagatc ttgatcgaga aacttcaacg
     3361 gcgacgcgga ataaagagat cggcgagaaa cgagggaact gggcgagatc g
//
LOCUS       DASUQP010000057         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_981018,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000057 DASUQP010000000
VERSION     DASUQP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981018"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFY74_02500"
     rRNA            <1..271
                     /locus_tag="VFY74_02500"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            444..558
                     /gene="rrf"
                     /locus_tag="VFY74_02505"
     rRNA            444..558
                     /gene="rrf"
                     /locus_tag="VFY74_02505"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            808..1290
                     /locus_tag="VFY74_02510"
     CDS             808..1290
                     /locus_tag="VFY74_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5930611.1"
                     /translation="MAKLSAGILLYRRTAPGIELLLVHPGGPFWANKDRGAWSIPKGE
                     YAEGEDPLDAAKREFAEELGAAPPARPFLNLGALKQPTRKVVTVFAVEGEFNPATLNA
                     NRFELEWPPKSGRKQTFAEVDRAQWFKPAEARKRILAGQAPFIDRLLECLGLGSSGRR
                     "
     gene            1535..>2474
                     /locus_tag="VFY74_02515"
     CDS             1535..>2474
                     /locus_tag="VFY74_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5930612.1"
                     /translation="MTVTGPARRRGAPLCAVQALAATLFAAVLLPAQGALAQGFNPYS
                     SFTYSGPVTPEEKARREQVRTDLYDRLSPAYRLDVPLVSEASIAAIELAIQRYHEIAA
                     SGGWPLTPQKVTLRPGDTSSEIAAIRQHLVIEGDLAPGGRQSGTFDREFLEGLSRYQI
                     RNGLRVSGFVDQRTLAALNVPAGERLAQLETNLKRLKGLMSINKAPRYVLVNVPAFFA
                     QAVERGTLALETNVITGKPARATPQIAAKIVEVNFYPTWSVPEIVAQQDLIPKIRKDP
                     DYFTAEHFTVMTDWKSPPLDPATVDWFSPAVESYKFR"
BASE COUNT          477 a          760 c          756 g          481 t
ORIGIN      
        1 tgccgtaggt gtaggagaat tgagaggatc tgtccttagt acgagaggac cgggatggac
       61 gcacctctag tggacctgtt gtcgcgccag cggcatagca gggtagccaa gtgcggaagg
      121 gataaccgct gaaagcatct aagcgggaaa cccacctcga aacgagttct cccttgagag
      181 ccgtggaaga ccaccacgtt gataggctgg gtgtggaagc gcagcaatgc gtgtagctaa
      241 ccagtactaa tcgctcgatc ggcttgattg ctctcattac cagcgcctat caggtgtcag
      301 atatcaggtg tcagaaatca gatatcaggt atcagacaac ggatggcgat tgacaaagac
      361 cagatttgca gaaatcagaa acggtttcat ttctgatttc tgatcactga tttctgtccc
      421 ctgacatgcc tgtccttcgc tggcctggtg attttggcgg agcgtgaaca cccgatccca
      481 tcccgaactc ggccgtgaaa agctccagcg ccgatggtac tgcgtcttaa gacgcgggag
      541 agtaggtcgt cgccaggctt gccaaggacg ggctgccaga gccctcatcg agatacgcgt
      601 gaaccgcgtc ccctctttca cgatcgttgg tcataagccg ccgctggaga attccgcggc
      661 ggcttttttg tgggcgctac cgcgcttcgc gctgcttgag cgcgtttttg tggcgctacc
      721 gcgcttcgcg ctatttgagc ccgtttttgt ggcgctaccg cgcttcgcgc tacttgagcg
      781 catgcgattt gcgcctaggg tccggtgatg gccaagctta gcgccggcat cttgttgtat
      841 cgccgcaccg cgccggggat cgagcttctc ctcgtgcacc ctgggggacc cttctgggcg
      901 aacaaggatc gcggcgcctg gtcgatcccg aagggcgaat acgcggaggg cgaagacccg
      961 ctcgacgcgg caaagcgcga gttcgccgag gagctcggcg ccgcgccgcc ggcccgtccc
     1021 tttctcaatc tcggcgcgct caagcagccg acccgaaaag tggttacagt cttcgccgtt
     1081 gaaggcgaat tcaatccggc aacgctcaac gccaatcggt tcgagcttga atggccgccg
     1141 aagagcggcc gcaagcaaac cttcgcggaa gtcgatcgcg cgcaatggtt caagcctgcg
     1201 gaagcgcgca agagaatcct ggcggggcaa gcgccgttca tcgatcgcct gctcgaatgt
     1261 ctcggcctcg gctcgagcgg ccgacgttaa cgaatcttga tcctcgtcat cctgaggtgc
     1321 gacctgcctg cgcgaagccg gggcgaagcg gagggatgac atcaggtgta tttgcgtcat
     1381 tcgagggccg ctttcggcgg cctcctcagg atgacgatat cctctttggc cattgccgag
     1441 gcctttcgga tcgtccatct tttgacacca agacctgaac tatgctgcgt tagggcgaat
     1501 tggagcacgg caggcgaggg acgctttccg caacatgacc gtgacagggc cggcaagacg
     1561 gcgcggcgcg ccgctttgcg ccgtgcaagc gctcgcggcg acattgtttg ccgccgtgct
     1621 gctgccggcg cagggagctt tggcgcaagg gttcaatcct tacagcagct tcacctatag
     1681 cggccccgtc actcctgaag agaaggcgcg ccgtgagcag gttcgcaccg acctctatga
     1741 tcggctgagc ccggcctaca ggctcgacgt gccgctcgtc agcgaggcct cgatcgccgc
     1801 gatcgagctc gccattcagc gctaccatga gatcgcggcg tccggcggct ggccattgac
     1861 gccgcagaag gtgacgctgc gccccggtga tacgagctcg gagatcgccg ccatcaggca
     1921 gcatctcgtc atcgaaggcg acttggcgcc cggtggacgc cagagtggaa ccttcgacag
     1981 ggaatttctc gagggtctct cccgctacca gatcaggaac ggccttcgcg tcagcgggtt
     2041 cgtcgatcag aggacgttgg ccgcgctcaa cgtgccggcc ggagaacgcc tggcccagct
     2101 cgagaccaac ctcaagcgcc tcaaaggatt gatgagcatc aacaaggcgc cgcgctacgt
     2161 gcttgtcaac gtcccggcct tctttgccca agccgtcgag cgcggaactc tcgcgctcga
     2221 gaccaacgtt atcaccggca agcccgcccg ggcgacgccg caaatcgcgg ccaagatcgt
     2281 cgaggtcaat ttctacccga cctggagcgt gccggagatc gtggcgcagc aggatctcat
     2341 tcccaagatc aggaaggatc ccgactattt caccgccgag cacttcaccg tcatgaccga
     2401 ctggaagtcg ccgccgctcg atcccgccac cgtcgattgg ttctcgccgg cggtcgagag
     2461 ctacaagttc cgcc
//
LOCUS       DASUQP010000058        26479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_164021,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000058 DASUQP010000000
VERSION     DASUQP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 26479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26479
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164021"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VFY74_02520"
     CDS             <1..1285
                     /locus_tag="VFY74_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5930613.1"
                     /translation="RAAEEALVQTIKQFGRSRNFDNGALISMETDGAVRAMVGGKDYG
                     ESQFNRASHAYRQPGSSFKGYVYLTALENGFTPETTVSDSPVSCGRWAPKNYSGGFRG
                     RMTLRMALAKSINTIAVKLSLQAGREKVLANLNKMGIKNIKKTCSLALGDNGMTPLEH
                     TGGYAVFAAGGLEVRPYAVEEIKTLTGGEVIYNHERDEPPRKQIFERKVIEQLNTMLQ
                     AVVLEGTGKAAQLDYTYSAGKTGTSSAYRDAWFLGYTGQYVTGVWLGNDDFTPMARVT
                     GGSYPAQTWHTYMMAAHDTDNIPQIAGLPVHPVQAEEQQRIAATLAQNATASAEVPAA
                     PAPESVKDMSSATRQVLEKLSGMLKDARQLTPSEGRPDRAQAPAAPAPGSQSAPSLAA
                     ANGTQPDSKPGNSPAPAAPQTVLSAGRDDNAATPR"
     gene            1355..1996
                     /locus_tag="VFY74_02525"
     CDS             1355..1996
                     /locus_tag="VFY74_02525"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5930614.1"
                     /translation="MLAPSRTTAWREDEAGHAMRSLVYFVGTFVVALILGIGSAWYMI
                     ERGSPLTTTKVGPWDGWISAGNPNADYYTRAHVARSGRLPLTSTVARYFVARTDSDGS
                     VLTADCAYLLTGMPLNARWWSLAVYDAYGGLIANPSDRYSFNSEELLRHADGSYRVNL
                     ARHARPENWLPSGEADQRLMLMLRIYSPRETDATGIGLVAGERLPKIERQSCE"
     gene            1987..2553
                     /locus_tag="VFY74_02530"
     CDS             1987..2553
                     /locus_tag="VFY74_02530"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX5930615.1"
                     /translation="MRIRLPGLYIALGLVLAGLIHIVAVLALPALAPKNGFARLASLG
                     PVNTIIHIPPAAPGQQPMPMMAPDIRYAFCRFDLAGGPVRLTAAIADELFLIALYTPD
                     GDNFYSVVGADMKRPQIDLIITTTEQVVEEAGVDAPESFDNIIVVNSPVKEGIALLRL
                     PLIGPSRASYAEQALKATSCGPYTKSSG"
     gene            complement(2563..2751)
                     /locus_tag="VFY74_02535"
     CDS             complement(2563..2751)
                     /locus_tag="VFY74_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930616.1"
                     /translation="MGIILEFRRSEGAAIPTAKPVNGSLGEIIIFPGVRIERGTWSDK
                     PVQAGSPSRRRVQRGRKK"
     gene            complement(2877..3224)
                     /locus_tag="VFY74_02540"
     CDS             complement(2877..3224)
                     /locus_tag="VFY74_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEX5930617.1"
                     /translation="MRVKSEVWVRAYLRRCQAEGVPVVIVRRGDEAAGAIFIRVDTLD
                     GNVSLFGPAPSGSAGSETDRRWVNCFTSRRTTAEEAARYLARQIEFDPDIWIVEVEDK
                     AGRHFLGDSVVEG"
     gene            complement(3249..3911)
                     /locus_tag="VFY74_02545"
     CDS             complement(3249..3911)
                     /locus_tag="VFY74_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX5930618.1"
                     /translation="MGVAAARLVFLGFLGLTGSIIYNALYLQAVQSTGPGAMGRPSAP
                     AIEAAKLQPVSTDLPPLAPQQGRDKLIVRAVQRELAARGYDVGPVDGTPSAKTRAAIS
                     AYEKTHTLPVTGTATDELLRHILLGDSVRGAGATGSVTGKAAPVKREPAKVKVVQQIL
                     ADLGYSPGRVDGTMGDATARAISAFQRDRKIPETGTITLELLNELERVTGRDAGKVVA
                     ER"
     gene            complement(4034..5275)
                     /locus_tag="VFY74_02550"
     CDS             complement(4034..5275)
                     /locus_tag="VFY74_02550"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX5930619.1"
                     /translation="MLGKEGVRGLIAYFLALVHDSARAGAPEPYRLLAESAPDMLTRH
                     DERGRVVFASAAAEPLFGEPAERIAGDGLFDRVHVADRPLYLTALSRALADNEQVAVA
                     FRIRRSGPAGAAEYVWVEMRCRPVGVSEGREPGQTCVIAVTRDISDRKAREAELVRAR
                     DAAEDASRAKTEFLANMSHELRTPLNAVIGFSDILSRELFGTFSEARYRDYARLIHES
                     SAHLLNVVDDILDMSKMEAGKFRIVKEPVDVGALVELCCDIMRPAAVERSLSLIADFA
                     PDVPALPADKTTCKQMLLNLIANAIKFTEPGGFVRVSARVSGANLELSVADNGIGIAE
                     QDLPRLGKPFVQASNAYGDGREGTGLGLAVVSGLARLHGGRLELDSALGKGTTATIFL
                     PYDETGEAREAPSKRPSVSAA"
     gene            5627..6022
                     /locus_tag="VFY74_02555"
     CDS             5627..6022
                     /locus_tag="VFY74_02555"
                     /inference="COORDINATES: protein motif:HMM:NF036461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5330 domain-containing protein"
                     /protein_id="HEX5930620.1"
                     /translation="MLFLIRTAFWLMIIVLLLPTDERQRSEVYGTAQATVNDVVTFCN
                     RNPETCAKGKDAFGVLVQKAQFGARMLIDMINGRPSTEAGEGGIDMPAPEQGSETQAI
                     FEPTRFDFAGSQDTLNPEDREEAWAGPGT"
     gene            6162..6623
                     /locus_tag="VFY74_02560"
     CDS             6162..6623
                     /locus_tag="VFY74_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX5930621.1"
                     /translation="MDTLSAARSLGFDEILTDFDLLGDWEDRYRYVIELGRGLEPLPE
                     GERSPATKVQGCVSQVWLSTQVDRTGGMPRLTFVGDSDAHIVRGLIAILFALYSGKTA
                     QEILSIDAGQRLGQLQLKEHLTPQRSNGLMAMVKRIRSDAGVALAQSQALH"
     gene            complement(6858..7409)
                     /locus_tag="VFY74_02565"
     CDS             complement(6858..7409)
                     /locus_tag="VFY74_02565"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5930622.1"
                     /translation="MLSARAFRAQLVMQGQRELFDYWLRSADGRRMPARSDLDPLKVP
                     RLLPHLGLIDLRAGLGEASFRLAGTRLHEIYGQEVTGMRADDVFSGDAASYWRRVHDR
                     VVDMGVPLHGVVRGPAQGRDHVVLFWLRLPLSEDGDRVDRILCYDVAAPMPEWRASQE
                     PSQVCHRRVYAQARSQPRRVQFG"
     gene            complement(7641..8048)
                     /locus_tag="VFY74_02570"
     CDS             complement(7641..8048)
                     /locus_tag="VFY74_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX5930623.1"
                     /translation="METNLEKNELVELTAEIVSAYVSNNTVVAGDLPGVIHNVFDALS
                     RASTATGQPAREELKPAVPVKKSVTPEYIICLEDGKKFKSLKRHLRTHYDLSPEDYRD
                     KWGLPHDYPMVAPNYAAARSDLAKRMGLGQRRR"
     gene            8542..8988
                     /gene="msrB"
                     /locus_tag="VFY74_02575"
     CDS             8542..8988
                     /gene="msrB"
                     /locus_tag="VFY74_02575"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5930624.1"
                     /translation="MSKTGSTEKSDPGKIAMSDQEWRSKLTPEQFYVTRQHGTERAFS
                     HPYHQEKDPGTYRCVCCGAPLFSSETKYNSGTGWPSFYAPANKDAVSEHSDRSFLMRR
                     TEVRCAKCEAHLGHVFPDGPEPTGARYCINGVALKLDKDAEGNRGT"
     gene            8999..11098
                     /locus_tag="VFY74_02580"
     CDS             8999..11098
                     /locus_tag="VFY74_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5930625.1"
                     /translation="MNDTLFRASMPCAARRPVTSTHHGIARVDDYAWLRAANWQAVIR
                     DPTLLDEKIRAYLETENAYSKAILADTEALQAALFAEMKGRIKEDDAGVPAPDGAFAY
                     YTRYVVGGQHPIFCRKPRDGGEERLLLDGNVLAEPYAYFRIIGVAHSPDHRLVAYAVD
                     TSGAEFYTLNVIEADTGALVDSRIADSNGTLEWSADSRHLFYIWLDDEHRPRRLLRHT
                     IGASEADLLVHEQADPGWFLGLGATQDRRYLQLSVHDHETAEISLIDAADPGAALRLV
                     APREPEHDYAVDHHDGRLIILTNSGGAEDYRIVEAPLATPGREHWREIEPHRPGRLIL
                     DVVAYKDFLVRLEREDGLPRIVVRRFADGEEHAIAFAEQAYALGISAGYEYETPILRF
                     TYSSMTTPAQVFDYDMNARTRTLRKTQEVPSGHDPSRYVTRRVMAAAKDGETVPISLV
                     FGKDTKLDGSAPLLLYGYGAYGMTIPAGFSTNALSLVDRGFVYAIAHVRGGKDKGYRW
                     YKDGKRAKKVNSFTDFIAAGEFLAEERYTSRGRIIAQGGSAGGMLMGAVANMAPDLLL
                     GIIAEVPFVDVLTTILDASLPLTPPEWPEWGNPIESEADYRTIAAYSPYDNVRALPYP
                     HILALAGITDPRVTYWEPAKWVAKLRAHATGGRLILLRTNMEAGHAGASGRFERLKEV
                     ALAYAFALKISSASEPL"
     gene            11163..11717
                     /locus_tag="VFY74_02585"
     CDS             11163..11717
                     /locus_tag="VFY74_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5930626.1"
                     /translation="MLLARDKSQLLIVDVQDKILDAMSGKNRVVERCVRLVRAARTLD
                     VPITLSEQYPQGLGPTVDPIREAVANAGFVADKVEFSCMRNATLRERLHELRRGGRPQ
                     VVVAGIEAHVCVMQTAIDLEGQGFEAFVVADAVSSRSKANCKRAMTRLLKEGADIVDS
                     EMVLFEWLERAGTPAFKELHALVK"
     gene            complement(11954..12388)
                     /locus_tag="VFY74_02590"
     CDS             complement(11954..12388)
                     /locus_tag="VFY74_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930627.1"
                     /translation="MGNLREVILLVATVCVSVMNANASRAEGDYWDPEARAKIKTVDD
                     ALALLVPELRKNLPMKVDEITTWLAIQALGPAVLYTYSFDLPKQDIEAIAGDLKNVTT
                     QNACNNKTWRMFLDAGARQRHLYVDESGLHAVQFDIDSNSCK"
     gene            complement(12421..12687)
                     /locus_tag="VFY74_02595"
     CDS             complement(12421..12687)
                     /locus_tag="VFY74_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930628.1"
                     /translation="MPLESARSRYRSAKKRKLGAANFTFTRQSLEIAAIALIWVMFFA
                     LNNLKYLIATQPVMLFLVPVWAIVAYLVMVFMPARWLRGESMTS"
     gene            13330..13932
                     /locus_tag="VFY74_02600"
     CDS             13330..13932
                     /locus_tag="VFY74_02600"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5930629.1"
                     /translation="MAFELPPLPYDYEALEPYMSAETLRYHHDKHHKAYVDNLNKLLP
                     GSGFEGKGLEEIVKASFGKNAGVFNNAAQDFNHIHFWPWMKKGGGGKKVPGAVLALID
                     RDLGGFDKFRTDFIEAGKTQFGSGWCWLAMKNGKLEVTKTPNGENPLVHGGWPLLGCD
                     VWEHSYYIDYRNERPKYLDAWFDHLINWDHVEEMLALAPK"
     gene            complement(14005..15528)
                     /locus_tag="VFY74_02605"
     CDS             complement(14005..15528)
                     /locus_tag="VFY74_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017274606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5930630.1"
                     /translation="MAKRKPAPKQAFDQSPAAAEAPADSVYIGKSVKPEFIYLRLANR
                     HGLITGATGTGKTVSLQGLAEGFSRHGVPVFCADVKGDLSGIAETGEPKSWVEARAEE
                     IGFTPEFQSYPVIFWDLFGRQGHPIRATVSELGPLLLSRLMDLSEAQEGVLNIAFKIA
                     GDEKLPLIDLKDLRSLLENLAARANELTTKYGNITKASVGSIQRALLVLEQQGSALFF
                     GEPALKLKDLMLHANDGRGYISVLAADKLMESPRLYATFLLFLLSDLFEKLPEVGDPD
                     KPKLVFFFDEAHLLFTDAPKALLEKVEQVVRLIRSKGVGVYFVTQNPLDIPDTVSRQL
                     GNRIQHALRAFTPLEQKAVKAAATTFRKNPKIDTERAIMELGIGEALVSTLDARGQPS
                     MAERTLVRPPNSRVGPISEAERRAVIDASPVRGIYDEREEQESASEILQQRTHRREEA
                     EAAQAEEKAEAKKASGSRSDSFWTTLGKTIIRTGVPLATKVLTDALKGRTRRGGGGF"
     gene            15662..17485
                     /locus_tag="VFY74_02610"
     CDS             15662..17485
                     /locus_tag="VFY74_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HEX5930631.1"
                     /translation="MAELSLARDFPPADEAAWKALVEEALKGSPFAALKSKTYDGIAV
                     EPLYPRAQEARPIAGRAPGSPWSVMQRIDHPDAKAANAQILDDLNNGANNITLVFEGA
                     VGDHGYALAATGTAISAALDEVHLDAGIAVDLDLGLQSKDAAGLLATLVKARALAPQA
                     VAIRFGFDPLGAIALAGASPLPWKDIAAMLAALVSDFAAQGFAGPFAAADGRVVHAAG
                     GSEAQELAFALASAIAYLRAMEQAGIAVEDARGLIYFRLAADQDQFLTIAKFRAIRKL
                     WARIEEACGLKPRPAFVSAETAWRMMTKRDPHGNIVRGTIAALGAAVGGADAVTVLPF
                     SAALGLPDPFARRIARNTQTILIEEANIHRVAEPAAGSGAIEALTDALGEAAWGLFQE
                     IEREGGAARALEAGSIQKDVAKVRAAREANVARRKDTLIGTSDFPNLAEDQADVLALP
                     RLAALSTDQRALTPIRLAEPFERLRDRSDQYRAKHGARPKVFLACLGRPADFNARASF
                     AKSLFEAGGIEAVEGDGANVAKRFRDSGAKLVCLCSSDKVYEREAAAAAKALREAGAK
                     RVYLAGKPGGHQAALEQAGIGSFLHQGCDTLQILTAAYDEI"
     gene            17490..19667
                     /gene="scpA"
                     /locus_tag="VFY74_02615"
     CDS             17490..19667
                     /gene="scpA"
                     /locus_tag="VFY74_02615"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX5930632.1"
                     /translation="MSRIPSFAEIGFAEIGFAAAGPDATAAARAEPWQTPEGIAVKPA
                     YDARDLKGLDFLDGMPGVPPYLRGPYPTMYVQRPWTIRQYSGFSTAEDSNAFYRRNLA
                     AGQQGVSIAFDLATHRGYDSDHPRVTGDVGMAGVAIDSIYDMRTLFDGIPLDQVSVSM
                     TMNGAVLPVLALFIVAGEEQGVPPEQLSGTIQNDILKEFMVRNTYIYPPAPSMRIVSD
                     IFAYSSKKMPKFNSISISGYHMQEAGATADLELAYTLADGIEYVRAGVAAGLDIDSFA
                     PRLSFFWAIGMHFFMEIAKMRAARLIWAKLIRDEFAPKDPRSLALRTHCQTSGWSLAA
                     QDVFNNVLRTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLMLQQESGT
                     IRIIDPWGGSFYVERLTYDLAARAMKHIAEVERIGGMAKAIEAGIPKRMIEQAATATQ
                     GRIDSRKQTIVGVNKYRSDSDAEIAILKVDNRAVRRRQLDKLARLKEERNEDEVRSAL
                     DALTRYAETGTGNLLKGAVQAARVKATVGEISYALEKVYGRHRAEVSAITGVYRAEMG
                     RMDGSMSRINDQIEAFETEHGRRPRILVAKMGQDGHDRGQKVIASAFADLGFDVDIGP
                     LFQTPEEAARQAVENDVHIVGVSSLAAGHLTLVPALREALEAQGRGDIMIVVGGVIPP
                     QDYPELYEAGAKAIFGPGTPIADAAIDLLQKLGTSTKSKQAAE"
     gene            19715..20722
                     /gene="meaB"
                     /locus_tag="VFY74_02620"
     CDS             19715..20722
                     /gene="meaB"
                     /locus_tag="VFY74_02620"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX5930633.1"
                     /translation="MHEADTAKKDIERIAEAIRAGDRVALARAITLVESAKPEDQARA
                     EGLLEALLPFTGKSIRVGISGVPGSGKSTLIDQLGLNLTAQGRKVAVLAVDPTSSRTG
                     GSILGDKTRMGRLAAEPNAFIRPSPAGDSLGGVTKTTRETVALAEAAGYDVILVETVG
                     VGQSETAVANMVDVFVVVAIPGAGDELQGIKRGLLELADVVAVNKAEGDQVERANRAA
                     MEYRTALHILSSSHSNWDPQVLTLSARDNQGLDALWTKIFERHAALSASGALDARRRD
                     QAAAWMREIFEQRLLAAFQGGKRAARHLQELENKVRAGEMTPSAAASELARLAGIKDA
                     D"
     gene            20776..21453
                     /locus_tag="VFY74_02625"
     CDS             20776..21453
                     /locus_tag="VFY74_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEX5930634.1"
                     /translation="MARPRVLITGFGPFPGFDENPSAWLVETLAKRQVSPEFELHARV
                     LPTEWEQVALVPRLFEALQPHVLIHFGVSEGAFAFRIEQSAHNQVARECDACGAMPKR
                     TVIRDGGPLRYDTPFPAEALVAHLMRNGIPARTSKSAGAYLCNFLYYHSLGWAHAQGN
                     APTVVFVHIPPWARRRRALGREMLLDGGAETLRFVLSCANGEKPLKTRAGSPISQTEA
                     GLRAKGA"
     gene            21457..22803
                     /locus_tag="VFY74_02630"
     CDS             21457..22803
                     /locus_tag="VFY74_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HEX5930635.1"
                     /translation="MVVGRREVLLGGVGLGLAVAAGPRAGLSQDPNALQACSTHRVAP
                     AGGAIDQTAALQAAADAAAASGTPLFLPAGIYSTGKLTLKSGTRIEGVPGETILRYRD
                     GGALIALEGVENVRIAGLVLDGDAKPLGERGSLLVATETKHLDVTNCRLTGSVADGVG
                     LRKVSGWIKDCEIVDIRKAGLVSEDASGLEIAHNHVRDCGGNGIVVSRSEHGEDATIV
                     SANRIERIAATSGGSSQSGNGISVVRASAVVINGNRIADCASSAIRANAGSNCQMIGN
                     SCTRLGEIALHAEFSVEGTLIANNLVDRAAAGISVANFARGGRLAVIQGNLIRNLFMR
                     KEADARGFGIAVEADSVVTGNVIEGAPAYGIMIGWGPHLRDVSVTDNLIRDALIGIGV
                     SIDPSAGTALITKNLIAGTKEGAIRAMSGPTLIGPDLATASAENFGNVAVYANVAR"
     gene            complement(23124..24977)
                     /locus_tag="VFY74_02635"
     CDS             complement(23124..24977)
                     /locus_tag="VFY74_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5930636.1"
                     /translation="MEFDPRGIALAVLVTCVSGPTLLPSHGESATQPQPGCAEARSIF
                     VAPMGMASTSDGELLIADRGLRAVVVVDPTTGACQLISSDQRGGGNSFNAPVAVVADT
                     KSYYVIDPGEGAVIRIEPRTGDRTELSGKHNGDGPQFSTARAIARLGGTAFVVFDIGL
                     NSVYRVDMESGDRSIVSSPTVGKGPPFVDPFGMVAASTGRLFVLDRGLQAILSVDLET
                     GDRKIVSGPERGEGPGFHSPTRVVLAPEDRLLVVDAALKSIVSVEPSSGDRTEVSGGE
                     AGAGPAFVMPVDLALVGGKLFVLDLGQRAILAVDPANGDRTITYKADALAVGTLLYPM
                     GIAATGSGEIVTVDPQRAAVIAVDPQTGVARVVSDAAHGDGMFFITPESVAITATGDL
                     IVGDATNIAMIRVDRRTGNRTVISGPARGVGPEIQDPIALALTDDMAVTTDPDLGAVI
                     AVDTATGDRKIVSGADFGSGPKFGVPIGIAALESRYYVSDPVSSAVFAVDPGDGKRMM
                     LRLVLPGSEVTLQSPVGIGADGKGNLVVTDLKTLTVYAIDPNRQQMHIVSGRKTESAL
                     RSSGHGASSKFLTACWLSVTRRFPQSCASIVPLAIGQRSCLAEQRGGFLPV"
     gene            complement(25210..26265)
                     /locus_tag="VFY74_02640"
     CDS             complement(25210..26265)
                     /locus_tag="VFY74_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX5930637.1"
                     /translation="MSASSNPVLVEVMRGRVVESRHSGAIAIADASGGLVLALGDVER
                     PVFPRSAVKALQALALVESGAADAFRLGDAELAVACASHSGDRVHIESVASLLDKAGL
                     DESYLACGAHWPVSDSAFRDVMRGGGRPLPIHNNCSGKHAGMLAACVHLGLDPSGYEH
                     PDHDLQVTIAGIISETCGVRLSRDGIGIDGCSVPTWALPLAALAQGFARFGTGTGLGS
                     ARARAAERLRRACFALPVLVAGEGRFDTIAMRALAPALFVKGGAEGVHCAALPELGLG
                     IALKMDDGAKRGAERALAEVLAALIPEARTALAEVLDGELLNWRGLRVGRVVASSALM
                     HGLAAITTAPRRKAGVP"
     gene            complement(26268..>26479)
                     /locus_tag="VFY74_02645"
     CDS             complement(26268..>26479)
                     /locus_tag="VFY74_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX5930638.1"
                     /translation="GNVHDEAGQSTYCASCGERVIGRDWYTITGWQLDEGGRCVSCGA
                     PCPGLFEPAPGNWGARRAPILIAAA"
BASE COUNT         4878 a         8664 c         8266 g         4671 t
ORIGIN      
        1 aagggcggct gaggaggctt tggttcagac catcaagcaa ttcggccggt cgcgaaactt
       61 cgataatggc gccttgatct cgatggagac cgacggagcc gtgcgcgcca tggtcggcgg
      121 caaggattac ggcgagagcc agttcaatcg cgcgagtcac gcctaccgcc agcccggctc
      181 atcgttcaaa ggctacgtct acctgaccgc gctcgagaac ggctttacgc cggagacgac
      241 cgtcagcgac agcccggtga gttgcggccg ctgggcgccg aagaactatt ccggcggctt
      301 ccgcggccgc atgacgcttc gcatggcgct cgccaagtcg atcaacacca tcgcggtgaa
      361 gctttcgctt caggccgggc gcgaaaaggt tctggcgaac ctgaacaaga tggggatcaa
      421 gaatatcaag aagacctgct cgctcgcgct gggcgacaac gggatgacgc ccttggagca
      481 caccgggggc tatgccgtgt tcgccgctgg tggcctcgaa gtgcgcccct acgccgtcga
      541 ggagatcaag acactcaccg gcggtgaggt catctacaat cacgagcgcg atgagcctcc
      601 gcgcaagcag atcttcgagc gcaaagtgat cgagcagctg aacacgatgc ttcaggcagt
      661 ggtgctcgag gggaccggca aagccgcgca gctcgattac acctactccg ccggcaagac
      721 cggaacgagc tcagcctatc gcgatgcctg gttcctgggg tacaccgggc agtacgtgac
      781 aggcgtgtgg ctcggaaatg acgacttcac gccgatggcg cgcgtcaccg gcggcagcta
      841 cccggcgcag acttggcaca cctacatgat ggcggcgcac gataccgata acattccgca
      901 gatcgccggc ttgcctgttc atccggtcca ggcggaggag cagcagcgca tcgccgctac
      961 gctcgcgcag aatgcgacgg ccagtgccga ggtgcccgca gcgccagcgc cggagagcgt
     1021 caaggacatg tcctcggcga cgcggcaagt cctcgaaaag ctgagcggca tgctcaagga
     1081 cgcccgccag ctgacgccga gcgaggggcg gccggatcgc gcccaggcgc cggcggcccc
     1141 tgcaccaggc tcgcagtcgg cgccaagctt ggcggcggcg aatggtactc agcccgactc
     1201 aaagcccggc aattcgccgg ctccggccgc gccgcagacc gttctttcgg cgggacgcga
     1261 cgacaatgcg gccaccccgc gttaggccgt tgcgggcctg cacggcttcg atgggtcaag
     1321 acgcttgacc agcagcttgc aagattcgcg gctcatgctg gcgccatcgc ggaccaccgc
     1381 ctggcgcgag gatgaggccg gccacgcgat gcgctcgctc gtctacttcg tggggacgtt
     1441 cgtcgtggct ctgatcctcg ggatcggatc ggcctggtac atgatagaac gcggatcgcc
     1501 tctgaccacg accaaggtcg ggccttggga cggctggatc agcgcaggca accccaatgc
     1561 cgactactat acgagggctc acgttgcccg cagcggccgg cttcccctca cctcgaccgt
     1621 cgctcgttac ttcgttgccc ggacggacag tgacgggagc gtgctgaccg cggactgcgc
     1681 gtatctcctc acgggcatgc ccctcaatgc gcgctggtgg tcgctcgccg tctatgacgc
     1741 gtatggcgga ctgattgcga atccttccga ccgctacagc ttcaacagcg aagagctgct
     1801 gcgtcatgcc gacggctcgt atcgcgtcaa tctggcgcgt cacgcgcggc cggagaactg
     1861 gctgccgagc ggggaggccg atcagcgcct gatgttgatg ttgcgcatct atagcccgcg
     1921 cgaaaccgat gccactggaa ttggcctcgt ggcgggtgaa cggctcccta agatcgagcg
     1981 tcaatcatgc gaataaggct ccccggcctc tatatcgctc ttgggctcgt gcttgcgggg
     2041 ctcatccata ttgtcgcggt gctcgccttg ccggcgctcg cgcccaagaa tggcttcgcc
     2101 cggctcgcct cgctcggtcc ggtcaacacg atcatccaca ttccgccggc agcgcccggg
     2161 cagcagccca tgccgatgat ggcgcccgac atccgctatg ccttttgccg attcgatctt
     2221 gccggtggac cggtgcgcct aaccgcggcg atcgccgacg agctctttct gatcgccctc
     2281 tatacgcccg acggcgacaa cttctattcg gtcgtcggcg ccgatatgaa gcggccccag
     2341 atcgatctca tcattaccac caccgagcag gtcgtcgagg aagcgggtgt cgacgccccg
     2401 gaatccttcg ataacatcat cgtcgtcaat tcgccggtga aggaaggaat cgctctcttg
     2461 cgcctgccct tgattggacc aagccgcgcg agctacgccg agcaggcgct caaggccact
     2521 tcctgcggtc cctacacgaa atcgtcgggc tagagcggcg ctctactttt tgcggccgcg
     2581 ctggacgcgt cttcgcgacg gcgagccggc ttgtacaggc ttgtcgctcc aggtgccgcg
     2641 ctcgatcctt acgccgggaa agatgatgat ctcaccgagc gagccattga ccggcttcgc
     2701 ggtcgggatc gccgctcctt cgctgcggcg aaattcaagg atgatcccca tgcttcgtct
     2761 cccctggagc ggcccgcgct tcgccatcgc aagtccgcat catccagtgc ttcattaagg
     2821 agcgggtaag gttaacgaac cgctaatacc gttgagctcc gccggcggat ttgccctcac
     2881 ccctcgacga ccgaatcacc gaggaaatgc cgccccgctt tgtcctcgac ctcgacgatc
     2941 cagatatccg ggtcaaactc gatctggcgg gcgagatagc gggctgcctc ctctgcggtc
     3001 gtccggcgcg acgtgaagca gttcacccac cgccgatcgg tctcgcttcc ggccgagccg
     3061 ctcggcgccg gcccaaaaag ggaaacgttg ccgtcgagcg tgtcgacgcg aataaagatg
     3121 gcgcccgccg cctcgtcgcc gcgccgcacg atcacgacgg gaacgccttc ggcctggcag
     3181 cggcgcaggt aagccctgac ccagacctcg cttttgaccc gcatggtggc gcgcgcttct
     3241 gccagcgctc agcgctcggc aaccaccttg cccgcgtcgc gcccggtgac tcgctcgagc
     3301 tcgttcaaaa gttcgagcgt gattgttccg gtctcgggaa tcttgcggtc gcgctgaaag
     3361 gcgctgatgg cccgcgccgt ggcatcgccc atcgtcccat cgacgcgacc cggcgaatag
     3421 ccgagatcgg cgagaatctg ctgcaccacc ttcacctttg cgggctcccg cttgacggga
     3481 gccgccttcc ccgtgaccga gcccgtcgcg ccggcgcccc gaacgctgtc gccgaggagg
     3541 atgtggcgca agagctcgtc ggtggccgtg cctgtcaccg ggagagtatg ggttttctcg
     3601 taggcggaga tcgccgcgcg tgtctttgcg cttggcgtac cgtcaacggg tccgacatcg
     3661 tagccgcgcg ctgcgagctc gcgctggacg gctctgacga tcagcttgtc ccgcccctgc
     3721 tgcggcgcca aaggcggcag gtccgtgctc acgggctgga gtttcgcggc ctcgatcgcg
     3781 ggcgcacttg gccgccccat cgctccgggc cccgtgcttt gcaccgcctg cagatagagc
     3841 gcgttgtaga tgatgcttcc cgtgaggccg aggaagccca gaaacacgag ccgtgctgcc
     3901 gctacgccca tcgcgaaagt gaacccaaaa attgcccggc cgagaggcct tccgttcggt
     3961 catcttggcg gccgaacata aagagatgtt aaacgcgact atgcgctttt ggcggcgccg
     4021 cctcgccgtt cccctatgct gccgaaaccg agggacgctt cgacggcgcc tcccgagctt
     4081 ctccggtctc atcatatggc aggaagatcg tagcggtcgt tccctttcca agcgcgctgt
     4141 caagctcgag acggccgcca tggagccgtg ccaaaccgct aacgacggcc aatccaagcc
     4201 cggtcccttc gcggccatcg ccgtaagcat tgctggcctg cacgaacggc ttgccgagtc
     4261 ttggcaagtc ctgctcggcg atgccaatgc cgttgtcggc gacgctgagc tcgagattgg
     4321 cgccgctcac gcgtgccgag acccgcacga agccgccagg ctccgtgaat ttgatggcgt
     4381 tcgcgatcag attgagcagc atctgcttgc aggtcgtctt atcggcaggg agcgcgggaa
     4441 cgtcgggggc aaaatccgcg atgagcgaga gcgaccgctc cacggccgca gggcgcatga
     4501 tgtcgcaaca aagctcaacc agagcgccca catcaaccgg ctccttcacg atcctgaact
     4561 tgccggcctc catcttcgac atatcgagaa tgtcgtcgac cacgttgagg agatgcgcgc
     4621 tgctttcgtg gatcagccgc gcgtaatcgc ggtagcgcgc ttcgctgaac gtgccgaaca
     4681 gctcgcggct gagaatgtcc gagaagccga tcacggcatt gagcggcgtg cgcaattcat
     4741 gactcatgtt ggcgaggaac tcggtcttgg cgcgacttgc gtcctcggcc gcatcgcggg
     4801 cgcgcacaag ttcggcctcg cgcgccttgc gatcggagat gtcccgggtc acggcaatga
     4861 cgcaggtttg gcccggctcg cgaccctccg agacgccgac agggcggcag cgcatctcga
     4921 cccagacata ttcggctgcg cctgccgggc cggaccggcg gatccgaaat gcgacggcga
     4981 cttgctcgtt atcggcaagc gcgcgtgaca aggccgtgag gtagaggggc cggtcggcga
     5041 catgcacgcg gtcgaaaagg ccgtcgccgg cgatcctctc ggccggctcc ccgaacagag
     5101 gctccgcggc cgccgacgcg aaaaccacgc ggccccgttc gtcatgccgg gtgagcatgt
     5161 ccggcgcgct ctctgcgagc aatcggtaag gctccggggc gccagcccgg gcggaatcgt
     5221 gcacgagagc cagaaaatag gcgatcagcc cccttacgcc ttccttgccc aacacgcaca
     5281 ctccgaacgg aaagacccgc tcgcgtcccc ggctgttctt acctcgggat tatcgtcctg
     5341 tgcgcttaag gaagcttaaa gcgctaacgc tggcgggtaa ggggttggac ggccgcgact
     5401 cgccttttct taccgtgaga tcgatcgaat ttgaaacaat cttatcgtga acgtaactta
     5461 gtcttactaa acagataata ccttgcatct cgaaatcgag cagaattttc gattcaaatt
     5521 ttatggattg cagtgaacgt cccttatttt ctctgcgcta tattcgcgcg acttaggaaa
     5581 agatagcggc gcaaaagccg caggatttaa cagagagggc aaagcaatgc tgttcctgat
     5641 ccggacagcc ttctggctga tgatcatcgt gcttttgctg ccgaccgacg agaggcagcg
     5701 cagcgaagtc tatgggacgg cgcaggctac ggtgaacgac gtcgtgacgt tctgcaatcg
     5761 caatccggag acctgcgcca agggcaagga cgcgttcggc gtgttggtgc agaaggcgca
     5821 gttcggcgcg cgcatgttga tcgacatgat caacgggcgc cccagcaccg aagcgggcga
     5881 aggcggcatc gacatgcctg cccccgagca aggctccgag acccaggcca tcttcgagcc
     5941 gacgcgtttt gatttcgccg gatcgcagga cacgctcaat cccgaggatc gcgaagaggc
     6001 ttgggcgggg cccgggacct aagtctccga gcttaggcgg tcttccgccc gtggggtgcg
     6061 cgcaattggg atggtctctg ccctttccct cccgattgcg cgccctcact ttttaaccca
     6121 agccggtcgc ggcggccggg tttcccgctt atattgatct catggatacg ctcagcgcgg
     6181 cgcgaagcct cggcttcgac gagatcctga ccgatttcga tcttctcggc gattgggagg
     6241 accgctaccg ctatgtcatc gagctcggcc gggggcttga gcccctgccc gagggcgagc
     6301 gttcccccgc gaccaaagtg caaggctgcg tcagccaggt ttggctctcg acgcaggtcg
     6361 accgaaccgg cgggatgccg cggctcactt tcgtcggcga cagcgacgcc cacatcgtcc
     6421 ggggcctgat agcgatcctc ttcgcgctct attccggcaa gacggcgcaa gaaatcttga
     6481 gcatcgacgc cggccagagg ctcggccagc tccagctcaa ggagcacctg acgccgcagc
     6541 gatcgaacgg gctgatggcc atggtgaagc gcatccggag cgacgccggc gtcgcgctcg
     6601 cccaatctca agccctgcat tgaggcattg cgtcctacct ccctcgtggg gaggtccgac
     6661 gacgccgtta ctcttgcgct atcgctccgc tgcttgagcg caaggcgccg tcggggtggg
     6721 ggcttgcgct tcaaggggcg gtcgcgagtt gcccacccgg ctcagctttc gctgcgctca
     6781 agcttcgcca ctcttcccct cacgggggga gggagggcaa taatccgggc tcactgcatt
     6841 tgccccaagc tcgcggctta gccgaattgc acccggcgcg gttgcgagcg tgcttgcgcg
     6901 taaacgcgcc gatggcaaac ttgcgaaggc tcttgcgaag cgcgccactc cggcatgggg
     6961 gctgcgacgt cgtaacagag aatacgatcg acgcgatccc cgtcttccga cagcggcaat
     7021 ctcagccaaa acagcacgac atgatcccgg ccttgagccg ggcccctgac cacgccgtgc
     7081 agcggaacac ccatgtccac cacccggtca tggacgcgac gccaatagga tgcggcatcg
     7141 ccggagaaga catcgtcggc gcgcatgccc gtgacttcct gtccgtagat ttcgtggagg
     7201 cgcgtcccgg cgagccggaa cgaggcctcg ccgagaccgg ccctgaggtc gatcagtccg
     7261 agatggggaa gcaggcgcgg aactttgagc gggtcgaggt ccgaacgggc cggcatgcgc
     7321 cgtccatccg cagaccggag ccagtaatcg aagagttcgc gctggccctg catcacgagc
     7381 tgggcccgaa aggcgcgtgc ggacagcatt tccttccccc gccgcaaggt cgcgagcagc
     7441 cccgcgaatc gcttgccgtt cttcgactat gtggagttcg gccgcgcctt gcaagccaag
     7501 aaagccagat tcactaggaa tcacaaaggc ttgtggtaac acttatggcg cctacccgat
     7561 cttggggaga agaaatccgg gcgttgcgcg cgcgggaagg ctccgcgcca acgcggcacc
     7621 tgtgcgtttt tcgcagaaga ctatcggcgc ctctgcccaa gacccatgcg cttggcgaga
     7681 tcggatcggg ctgcggcgta gtttggcgcc accatcggat agtcgtgggg caggccccac
     7741 ttgtcgcggt aatcctccgg cgacaggtcg taatgcgtgc gcaaatgacg cttcagcgat
     7801 ttgaactttt ttccgtcctc gagacagata atgtattcgg gcgtgaccga tttcttgacc
     7861 gggacggcgg gtttcaactc ttcacgtgcg ggctggccgg tcgccgttga ggctctgctc
     7921 agcgcatcga aaacgttgtg gatcacacca gggaggtcgc ccgcgacaac ggtgttgttg
     7981 ctcacatagg cggaaacgat ctcagctgtg agttcaacga gttcgttctt ctccagattt
     8041 gtttccatga atagctcctc gatcctggcc ggtctcggtt caaacctgcc cccaaagccg
     8101 tgctaagggg gcgggcgcgt cttccctgca cttcggactc ttcagtcacc agattacgcc
     8161 gtttttgtga cgtgaagtta caattctctc aactacaaaa tatagatcgg cgccgatgtt
     8221 tgaagctcat ttgtgattcg gcgctagttt ccccgtgaat ccgctcggca gctttgctga
     8281 gcgaaaacta gcgggccacg gccttgataa acgaagctca ggcgttcgct tacctaaacc
     8341 ggttcccaca accactcaag attgataggc accccgtttt gactattttg cgattccccc
     8401 gctttgcccg cactggacaa agatgcaact tcatcgggaa tcaagcttgt gtgagaggcg
     8461 ggctgcctct cttcgacgcc tcatccccga tacttatatt gcgatcaaat aaatgccgac
     8521 ctacccgaaa gtgttccatc gatgagcaaa actggcagca ctgaaaaaag cgacccgggc
     8581 aagatcgcaa tgtcggacca ggagtggcgc agcaagctta cgcccgagca gttctacgta
     8641 acccggcagc acggaaccga gcgtgccttc agccacccct atcaccagga aaaggacccc
     8701 ggcacgtatc gctgcgtctg ctgcggcgcg cctctgttca gctccgagac caaatacaat
     8761 tcaggcactg gctggccgag tttctatgca ccggccaaca aagacgcggt cagcgaacat
     8821 tcggaccggt cgttcttgat gcggcgcact gaggttcgct gcgccaaatg tgaggctcat
     8881 ctcggtcatg tgtttccgga cggacccgag ccgacgggcg cgcggtattg catcaacggt
     8941 gtcgccttga agctcgacaa ggacgccgag ggcaacagag gcacgtaaag ctcaaggcat
     9001 gaatgacacg cttttccgcg caagcatgcc ttgcgccgcc cggcgacccg tgacctcgac
     9061 ccaccacggg atcgcgcggg tggatgacta tgcgtggctc cgcgccgcga actggcaagc
     9121 ggtgatccgc gaccccactc tgcttgacga aaagatcagg gcctatctcg agacagagaa
     9181 cgcctacagc aaggcaatcc tcgcggacac cgaggcgctt caggcggcgc tcttcgcgga
     9241 gatgaagggg cggatcaagg aggacgatgc cggggtgccg gctcccgatg gggccttcgc
     9301 ctactacacg cgctacgtcg tcggcggcca acatccaatc ttctgccgca agcccaggga
     9361 tggcggtgaa gagcggctcc tgctcgacgg caatgtgctg gcggagcctt acgcttattt
     9421 ccgcatcatc ggcgtcgcgc acagtccgga tcaccggctc gtggcctatg ccgtcgacac
     9481 cagcggcgcc gagttctaca ccctgaatgt gatcgaggcg gacaccggcg cgctcgtcga
     9541 ttctcgcatc gccgacagca acggcacgct ggaatggagc gccgacagcc ggcacctttt
     9601 ctacatctgg ctcgatgatg agcaccgtcc gcgccgtttg ctgcgtcaca cgatcggcgc
     9661 tagcgaggcc gatctcctcg tccacgagca agccgacccg ggctggttcc tcgggctcgg
     9721 cgcaactcag gatcgtcgct acctccagct gagcgtccac gaccacgaaa ccgcggagat
     9781 cagcctgatc gacgccgccg atcccggcgc cgcccttcgg ctcgtcgcgc cccgcgaacc
     9841 cgagcacgac tacgccgtag accatcacga cggccggctc atcatcctca ccaactccgg
     9901 aggcgccgag gattaccgca tcgtcgaagc gccgctcgcc acccccggtc gcgagcattg
     9961 gcgcgagatc gagccgcaca ggcccggccg gctcatactc gatgtcgttg cctacaagga
    10021 ctttctcgtg cggctcgagc gcgaggacgg gttgccccgc atcgtcgtgc gccgcttcgc
    10081 cgatggcgag gaacacgcca tcgcgtttgc cgaacaggct tatgcgctcg gcatctccgc
    10141 cggttacgag tacgagaccc cgatcttgcg cttcacctat tcctcgatga cgacgccggc
    10201 gcaagtgttc gactacgaca tgaacgcccg cacgcggacg ctgcgcaaga cccaggaggt
    10261 tccgagcggt cacgatccgt cgcgttacgt cacgcgccgc gtcatggcgg cggccaagga
    10321 cggcgagacg gtgccgatct cgctcgtgtt cggcaaggac acgaagctcg acggcagcgc
    10381 gcctctcctt ctctacggct atggcgctta cggcatgacc attccggccg gcttctccac
    10441 caatgcgctc agcctcgtcg atcgcggttt cgtctacgcc atcgcccatg tccgcggcgg
    10501 caaggacaag ggttatcgct ggtacaagga cggcaagcgc gcgaagaagg tgaacagctt
    10561 caccgatttc atcgcggcgg gcgagttcct cgcggaagag cgctacacgt cacgcggacg
    10621 gatcatcgcc caaggcggca gcgcgggtgg catgctcatg ggcgcggtcg ccaacatggc
    10681 gcccgacctg ttgctaggga tcattgccga ggtgcccttc gtagacgtgc tcacgacgat
    10741 tctcgatgcg agcttgccgc tgaccccgcc ggaatggccc gaatggggga acccgatcga
    10801 gagcgaagcc gactacagga ccatcgccgc ctactcgccc tatgacaatg tgcgggcgtt
    10861 gccctatccg catatccttg cgctcgcagg tatcaccgac ccccgcgtga cctattggga
    10921 accggccaaa tgggtggcga agctccgcgc gcatgccacg gggggtcgcc tcatcctgct
    10981 ccgcaccaat atggaggcgg gccatgccgg cgcctcggga cgcttcgagc gcctgaaaga
    11041 agtggcgctg gcctacgcct ttgcgctcaa gataagctcc gcctcggaac cgttatagtc
    11101 tggcgttcga ccgcatcgcc ggtctatgaa gcgggggcga gtcccctcac attacgaatg
    11161 ccatgctgct cgcccgcgac aagtcccagc ttctcatcgt cgacgtccag gacaagatcc
    11221 tcgacgcgat gtctggcaag aaccgcgtgg tcgagcgctg tgtgcggctc gtgcgcgcgg
    11281 cgaggacgct cgatgtgccg atcacgcttt cggagcaata tccgcaaggc ttgggaccca
    11341 cggtcgaccc catccgcgag gcggtcgcca atgccggctt cgtggccgac aaggtcgaat
    11401 tctcctgcat gcggaacgcg acgctccgcg agaggctgca tgagcttcga cgtggaggcc
    11461 ggccccaagt cgtcgtcgcc gggatcgagg ctcatgtctg cgtgatgcag accgcgatcg
    11521 atctggaggg acagggcttc gaggcttttg tagtcgccga cgcggtcagc tcgcgctcga
    11581 aagcgaactg caagcgcgcg atgacgcggc tcttgaagga aggcgccgat atcgtcgaca
    11641 gcgaaatggt cttgttcgag tggctcgagc gcgccggcac gccggcattc aaggagctgc
    11701 acgccctcgt caagtagctc gcccggtcac tacccgcctc atgctgagga gcgctccgaa
    11761 gaagcgcgtc tcgaagcatg gtgcccggaa ggcatccttc gagacgccgc cttcggcggc
    11821 tcctcaggat gaggtttgca tttggacgca actccccctc acacggctcg ccactcgacg
    11881 cggctcgccg tcctctccca ctaggaaagc gtcaggaact cgcgagagta acgctatagg
    11941 caatcacgat acgtcatttg cacgaattgc tgtctatgtc gaactggaca gcatgtaagc
    12001 cgctctcgtc tacgtacaga tgcctttggc gtgcgcccgc atcaaggaac attcgccaag
    12061 tcttgttgtt gcaggcgttc tgcgtggtca cgttcttaag gtcgcccgcg atagcctcaa
    12121 tgtcctgttt cggcaaatcg aagctgtatg tgtagagaac ggctggacca agagcttgaa
    12181 tggcgagcca ggtcgttatc tcgtcaacct tcatcggaag gttcttcctg agttcaggaa
    12241 ccaacaaagc caaagcatca tcaacagtct taatttttgc tctagcttcc gggtcccaat
    12301 agtcaccctc cgctctcgat gcgttcgcgt tcatgactga tacgcaaacc gtggcgacga
    12361 gaagaatcac ctcacgcaga tttcccatgc tcgcagcctc tcacgacgtt gctgctggcg
    12421 ctaacttgtc atcgattcgc ctctcagcca tcttgcaggc atgaagacca taaccagata
    12481 ggcgacgatt gcccagacgg ggactaagaa caacatcacc ggctgcgttg cgatcaggta
    12541 tttcaagttg ttcagggcga agaacatcac ccagattaag gctattgcgg cgatttccag
    12601 agattgacga gtgaaggtga aattagccgc gccgagtttg cgctttttgg cggacctgta
    12661 tctggagcgc gctgactcca gcggcaaacg taaggaagcc caatacaagt gcgattgtag
    12721 accccaaatg ccgcctcacc ccaatccatc gcgcgtccat gagtatttcc taggatgccg
    12781 cgaagtcgcc aataacctcg gcttcgtgac ggaggatggc taggggaagg gggctgactg
    12841 cgctcgtctg ccctgctggg aggaaagaag aagcggctcg ctcgaccgcc tgaaggtttg
    12901 caatacacat gcagcgtcag caacaaggct cggggtgcga gcagcaagag cgcaaacagc
    12961 atctttgggc tgatccttct agacgcctcg ctgtgggtcg cgtggactat caagcctgac
    13021 cccccgcacc gacagcatcg catgaaatga aagagggcgg ctagtcccct gaactgatgc
    13081 tgcaagcgcg gttcatgcgg ggttcaggcg acggcgagca aattagaggg cgtcagatcc
    13141 ctgcactgat gtaagcgaac cttcctcggc accgggcgcg gggttcaggg gctccgcgcc
    13201 caccgttttt cgaactgatc tcgcataacc ctacgcgcgc cgcgcgatgg ctcggcttgc
    13261 ctcccctcgc cggaaccttt agctttctgc ggactttccc cacctctttc aacttgcggg
    13321 agcatcgccg tggccttcga acttcccccc ctgccctacg actatgaagc gctcgagccc
    13381 tacatgtcgg ccgagacgct ccgctatcac cacgacaagc atcacaaagc ctatgtcgac
    13441 aatctgaaca agctcctgcc gggctccggc ttcgaaggca aggggctcga agagatcgtc
    13501 aaggcaagct tcggcaagaa cgccggcgtc ttcaacaacg cggcccagga tttcaaccac
    13561 atccatttct ggccgtggat gaagaaaggc ggcggcggca agaaggtgcc gggcgcggtt
    13621 ctcgcgctga tcgaccgcga cctcggcggc ttcgacaagt tccgcaccga tttcatcgag
    13681 gcgggcaaga cgcaattcgg ctcaggctgg tgctggcttg cgatgaagaa cggcaagctc
    13741 gaagtgacca agacgccgaa cggcgagaac ccgctcgtgc atggcggctg gccgctgctc
    13801 ggctgcgacg tgtgggagca ctcctactac atcgactatc gcaacgagcg gccgaaatat
    13861 ctcgacgcct ggttcgacca tctcatcaat tgggatcacg tcgaggaaat gctggcgctc
    13921 gcgccgaaat agctgagagg actgggcgca aggttcgcat ggtcgatcct gcaaaccccg
    13981 gtgaaggtcc gacctcaaac tgagctagaa tccgccgccg ccccgccgcg tgcggccctt
    14041 cagcgcatcc gtcagcactt tcgtcgccaa tggcactcca gtcctgatga tggtcttgcc
    14101 gagcgtcgtc cagaaactgt cgctgcgcga gccgcttgcc ttcttcgctt cggccttctc
    14161 ctcggcctgg gcggcttcgg cttcttcgcg ccgatgcgta cgctgttgca ggatctcgga
    14221 ggccgactcc tgctcttccc gctcgtcata aataccgcgg accgggctcg cgtcgataac
    14281 ggccctccgt tccgcctcgg agatcgggcc cacgcgcgag ttgggcggcc tgaccaaagt
    14341 gcgctccgcc atgctgggct ggcccctggc gtcgagcgtg gagacgagcg cctcgccgat
    14401 gccgagctcc atgatcgccc gctcggtgtc gatcttcggg ttcttgcgaa aggtggttgc
    14461 cgccgccttc accgccttct gttccagggg cgtgaacgcg cgcaaagcat gctggatgcg
    14521 attgccgagc tggcgcgaca cggtgtcggg aatgtcgagc gggttctgcg tgacgaagta
    14581 gacgccgacg cccttcgagc ggatcagccg caccacctgc tctacctttt cgagcagcgc
    14641 tttgggcgca tccgtgaata ggagatgcgc ctcgtcgaag aagaacacga gcttcggctt
    14701 gtcgggatcg ccgacctcgg gcagcttctc gaaaaggtcg gaaaggagga aaagaaggaa
    14761 ggtcgcgtag aggcgcgggc tttccatcag cttgtcggcg gcgagcacgc tgatatagcc
    14821 gcggccgtcg ttcgcgtgca gcatgagatc cttgagtttc agagccggct cgccgaagaa
    14881 gagagcggag ccctgctgtt cgagcacgag cagcgcgcgc tggatcgaac cgaccgaagc
    14941 cttggtgatg tttccgtatt tcgttgtgag ctcgttggcg cgggccgcca ggttctccaa
    15001 cagcgaccgc aagtccttga ggtcgatcag cggaagcttc tcatcgcccg cgatcttgaa
    15061 ggcgatattg agcaccccct cttgcgcctc gctcagatcc atgagccgcg agaggagcag
    15121 aggtccgagc tcggagactg tcgccctgat cgggtgcccc tgcctgccga acaggtccca
    15181 gaagatcacc ggataggatt gaaactcggg cgtgaagccg atctcctctg cccgcgcctc
    15241 gacccacgat ttgggttcgc ccgtctcggc gatgcccgat aggtcgcctt tcacgtcggc
    15301 gcagaacacg ggcacgccgt ggcgggagaa gccctctgcc aagccctgca gcgagacggt
    15361 cttgcccgtg ccggtcgcgc ctgtaatgag cccgtggcgg ttggcgagcc tgagatagat
    15421 gaactcgggt ttgacgctct tgccgatata gacgctgtcc gcgggggctt ccgccgctgc
    15481 cgggctctgg tcgaaagcct gtttcggcgc gggcttgcgc ttcgccatgt cttcctctcc
    15541 tcgattcccg ccagcactct agcttctgcc tagccgcgcg caaagagaac gctggcgcgg
    15601 cttccgcgcc gcttcgcctt catgctagga ggagacagga ccagcttaga gcttgacgca
    15661 catggcggaa ctttccttgg cccgggactt cccgcccgcc gacgaggcgg catggaaggc
    15721 gctcgtcgaa gaggcgctca agggctcgcc ctttgccgcg ctcaagtcca agacctatga
    15781 cggcatcgcc gtcgagccgc tctatccccg cgcgcaagag gcgcgcccga tcgccggccg
    15841 cgccccggga tcgccttgga gcgtgatgca gcggatcgat catccggacg cgaaggccgc
    15901 caacgcgcaa atcctcgacg atctgaacaa cggcgcgaac aacatcacgc tcgtgttcga
    15961 gggcgccgtc ggtgatcatg gctatgcgct tgccgccacg ggcaccgcga tcagtgccgc
    16021 gctcgacgag gtccatctcg atgccggcat tgcggtggac ctcgacctcg gccttcaatc
    16081 gaaagacgcg gcgggcctcc tcgccactct ggtcaaggcc cgcgcgctcg cgccgcaagc
    16141 cgtcgccatc cgcttcgggt tcgatccgct cggcgccatc gccttggcgg gggcgagccc
    16201 ccttccctgg aaagacatcg cggccatgtt agccgcgctc gtgtccgact tcgcggccca
    16261 aggcttcgcg ggaccgttcg ccgccgccga tggcagggtg gtccatgccg ccggcggctc
    16321 cgaggcgcag gagcttgcct tcgcgctcgc aagcgcgatt gcctatctcc gggccatgga
    16381 gcaagcgggc atcgccgtcg aagatgcgcg cggcctcatc tatttccggc tcgccgccga
    16441 tcaggaccag ttcctcacca tcgccaagtt ccgcgcgatc cggaagctgt gggcgcgcat
    16501 cgaggaggcc tgtggcctaa aaccccgtcc cgccttcgtc tccgccgaga ccgcctggcg
    16561 gatgatgacg aagcgcgatc cgcacgggaa catcgtgcgc ggcacgatcg cagcactcgg
    16621 cgccgcggtc ggcggcgccg atgctgtgac ggtgctgccg tttagcgctg cgctcgggct
    16681 ccccgatccc ttcgcccgcc gcatcgcccg caacacccag accatcctga tcgaagaagc
    16741 gaacatccac cgcgtcgccg aacccgccgc aggctccggc gcgatcgaag cgctcacaga
    16801 tgcgctcggc gaagcggcct ggggtctgtt tcaggaaatc gagcgcgagg gcggcgcagc
    16861 gcgcgcgctc gaggccggtt cgatccagaa ggatgtggcc aaggtcaggg cggcgcgcga
    16921 agccaatgtc gctcgccgga aggacacgct gatcggcacg agcgactttc ccaatctcgc
    16981 cgaggatcaa gccgatgtgc tcgcgctgcc gcggctcgcg gccctgtcga ccgaccagcg
    17041 cgcgcttacc ccgatcaggc tcgccgagcc tttcgagcgc ttgcgcgacc ggagcgacca
    17101 gtatcgcgcc aagcacggcg caaggccgaa ggtctttctc gcctgtctcg ggcgtcccgc
    17161 ggacttcaat gcgcgggcaa gtttcgccaa aagcctgttc gaggcgggcg gcatcgaggc
    17221 cgtcgaagga gacggcgcca acgtcgcgaa acgattcagg gattcgggtg cgaagcttgt
    17281 ctgcctgtgc tcctcggaca aggtctatga gcgcgaggcc gccgccgcgg ccaaggcgct
    17341 gagggaagcc ggcgcgaagc gcgtctacct cgccggcaag cctggcggtc atcaggccgc
    17401 gttggaacaa gccggtattg gcagtttcct tcatcagggc tgtgatacgc ttcaaatcct
    17461 gaccgctgcc tacgacgaga tctgagcgaa tgtcgcgcat cccctccttc gccgagatcg
    17521 gcttcgccga gatcggcttc gctgccgccg ggccggatgc gaccgctgcc gcgcgcgccg
    17581 aaccctggca gacgccggaa gggattgcgg tcaagcccgc ctatgacgcg cgcgacctca
    17641 agggtctcga tttcctcgac ggcatgcccg gcgtgccgcc ttatctgcgc ggcccctacc
    17701 caaccatgta tgtgcagagg ccctggacca tccggcaata ttccggcttc tccaccgccg
    17761 aggattccaa cgccttctac cggcgcaatc tcgccgccgg ccagcaaggc gtttccatcg
    17821 ccttcgacct cgccacccat cgcggctacg attccgatca tccgcgcgtg accggcgatg
    17881 tcggcatggc gggcgtcgcc atcgactcca tctacgacat gcgcaccctg ttcgacggca
    17941 tcccgctcga tcaggtctcg gtctccatga ccatgaacgg ggcggtgctc cccgtgctcg
    18001 cgctcttcat cgtcgcgggc gaggagcaag gcgtgccgcc ggagcagctc tcagggacga
    18061 tccagaacga catcctcaag gagttcatgg tgcggaacac gtacatctat ccgcccgctc
    18121 cctcgatgcg catcgtctcc gacatcttcg cctacagctc gaagaagatg ccgaaattca
    18181 attcgatctc gatctccggc tatcacatgc aggaggcggg agcgacggcc gacctcgagc
    18241 tcgcctatac gctcgccgac ggcatcgaat atgtgcgtgc gggcgtggcc gccggtctcg
    18301 acatcgactc gtttgcgccg cggctctcct tcttctgggc gatcggcatg cacttcttca
    18361 tggaaatcgc caagatgcgc gccgcgcgcc tcatctgggc gaagctgatc agggacgagt
    18421 tcgcgccgaa ggacccccgc tcgctcgcgc tcaggaccca ttgccagacg agcggctgga
    18481 gcctcgccgc gcaggacgtg ttcaataacg tcttgcggac atgcatcgag gcgatggccg
    18541 cgacccaagg ccatacccag tcgctccaca ccaacgcgct cgatgaggcg ctcgcgctgc
    18601 cgaccgattt ctcggcgcgc atcgcccgca acacccagct catgttgcag caggaatcgg
    18661 gcacgatccg catcatcgac ccctggggcg gcagcttcta tgtcgagcgc ctcacctacg
    18721 atctcgcggc gcgcgcgatg aaacacatcg ccgaagtgga gaggatcggc ggcatggcga
    18781 aggcgatcga ggcgggtatc ccgaagcgca tgatcgagca ggccgcgacc gcaacgcaag
    18841 gccgcatcga ttcgcgcaag cagacgatcg tcggcgtgaa caagtaccgc tccgacagcg
    18901 acgccgagat cgccatcctc aaggtggaca atcgggccgt gaggcggcgg cagctcgaca
    18961 agctcgcgcg cctcaaggag gagcgaaacg aggatgaggt ccgctcggcc cttgacgcgc
    19021 tgacccgcta cgccgagaca gggaccggta acctgctaaa aggcgccgtc caggccgcgc
    19081 gcgttaaggc gacggtcgga gaaatctcct atgctctgga gaaggtctat ggccgccacc
    19141 gggcggaagt gtcggcgatt accggcgttt accgcgcgga gatgggccgc atggatggaa
    19201 gcatgagccg catcaacgac cagatcgagg ccttcgagac cgagcacggg cgccgcccgc
    19261 gtatcctcgt cgccaagatg ggccaggacg gtcacgatcg cgggcagaag gtgatcgcct
    19321 ccgcgtttgc cgatctcggc ttcgacgtcg atatcgggcc gctgttccaa acaccggagg
    19381 aagccgcccg ccaggcggtg gagaacgacg tgcatatcgt cggggtttcg tcgcttgccg
    19441 cgggccactt gacactggtg cctgcgctgc gcgaggcgct ggaagcgcaa ggccgcggcg
    19501 acatcatgat tgtggtgggc ggcgtgatcc caccgcagga ctaccccgag ctttatgagg
    19561 cgggcgccaa ggccatcttc ggccccggca cgccgattgc cgacgcggca atcgatctgc
    19621 tgcagaagct cggcacgagc acgaaatcga aacaggcggc agagtaggct tcaaccgctt
    19681 gcgttgaacc ttgtttgccc ctacgccgcc tcccatgcac gaagccgaca cggccaagaa
    19741 ggacatcgag cggattgccg aagcgatccg cgccggcgac cgcgtggcct tggcgcgcgc
    19801 catcacgctt gtcgaaagcg ccaagcccga ggatcaggct cgcgccgagg ggctgctcga
    19861 agcgctcctg cccttcaccg gcaaatccat cagggtcggc attagcggcg ttccgggctc
    19921 gggcaagtcg acgctcatcg atcagctcgg gctgaacctg accgcgcaag gccgcaaggt
    19981 tgccgtgctc gccgtcgatc cgacctcatc gcggaccggc gggtcgatcc tcggcgacaa
    20041 gacgcggatg ggacggctcg ccgccgaacc gaacgccttc atccgtccct cccccgccgg
    20101 cgacagtctg ggcggcgtga ccaagaccac gcgagagacc gtggcgctcg ccgaagccgc
    20161 gggctacgac gtgattctcg tcgagacagt gggtgtcggc cagtccgaga cggcggtggc
    20221 caatatggtc gatgtgttcg tggtggtagc gatccccggc gccggcgatg agctccaggg
    20281 catcaagcgc ggcctgctcg agcttgccga cgtcgttgcc gtgaacaagg ccgaaggcga
    20341 ccaggtcgag cgtgccaacc gcgcggcaat ggagtaccgc accgcgctcc acattctctc
    20401 ttcaagccat agcaactggg acccgcaagt gctgacgctc tcggcgcgcg acaaccaggg
    20461 attggatgcg ctatggacga agatcttcga acgccatgcg gcgctttccg cttccggcgc
    20521 actcgatgcg cgccgccgcg accaggcggc ggcttggatg cgggagatct tcgagcagcg
    20581 cctgcttgcc gccttccagg gcggcaagcg cgccgcgcgc catttacagg agctggagaa
    20641 caaggttcgc gccggcgaga tgacgccgtc cgccgcggcc tccgagcttg cgcgcctcgc
    20701 cggcatcaag gacgcagatt aggcatttcc tgaaagtgct tccctccggc atgaggccct
    20761 tgtaagccgt gaaccatggc tcggccgcgc gtcctcatca cgggctttgg ccccttcccg
    20821 ggttttgacg agaacccatc agcctggctc gtcgagacat tggcgaagcg gcaggtatcg
    20881 cccgagttcg agctgcacgc ccgcgtgttg ccgaccgaat gggaacaggt cgcgctcgtt
    20941 ccccgcctct ttgaggcgct gcaaccgcat gtgttaatcc attttggtgt gagcgagggc
    21001 gccttcgcct tccgcattga acagtcagcc cataatcagg tggcgagaga gtgcgatgca
    21061 tgcggcgcga tgccgaagcg caccgtgatt cgtgacggcg ggcccttgcg ctacgacacg
    21121 ccgtttccgg ctgaggcgct cgtggcgcat ctcatgcgaa acggcatccc ggcaaggacg
    21181 tcaaagtcag ccggcgccta tctctgcaat tttctctact accattcgct cggatgggcg
    21241 cacgcccaag gaaacgctcc gacggtcgtc ttcgtgcata tcccgccctg ggcccgaagg
    21301 cgcagagcct taggccgcga aatgttgctt gacggcgggg ccgagacatt gcgcttcgtt
    21361 ctaagttgcg caaacgggga aaagccgttg aaaacgcgag ccggctcgcc aatctctcaa
    21421 accgaggcgg gcttgagggc aaagggtgcg taagccatgg ttgtcggtcg ccgcgaggtt
    21481 ttgttgggcg gggtcggtct tggccttgcc gtcgcggcag gcccccgcgc gggcctttct
    21541 caagacccca acgctctgca agcttgcagc acgcacaggg tggcgcccgc gggcggcgcc
    21601 atcgaccaga ccgcggcgct tcaagccgcc gccgacgcgg ccgcagcgtc cggcacgccg
    21661 ctgtttctac ccgcgggtat ctactcgacc ggcaagctca cgctcaaatc cgggacccgc
    21721 atcgagggcg tgccgggaga gaccatcctt cgctatcgcg atggcggcgc gctgatcgcg
    21781 ctcgaaggcg tcgagaacgt caggatcgcc gggcttgtcc tcgacggcga cgcaaagccg
    21841 ctcggggagc gcggatcgct tcttgtagcg accgagacga aacatctcga cgtgacgaac
    21901 tgccggctga ccggcagcgt ggcggatggc gtcggcttgc gcaaagtgtc gggctggatc
    21961 aaggattgcg agatcgtcga tatccgcaaa gcgggactgg tcagtgagga cgcaagcggg
    22021 ctcgagatcg cccataacca tgtacgcgac tgcggcggca acggcatcgt ggtgtcacga
    22081 tcggagcacg gcgaggatgc caccatcgtg tctgcaaatc gcatcgagcg catcgccgcg
    22141 accagcgggg gcagcagtca gagcggcaac ggaatcagcg tcgttcgcgc gagcgccgtc
    22201 gtgatcaacg gcaaccgcat cgccgattgc gcctcctccg cgatccgcgc caatgccgga
    22261 tcgaactgcc agatgatcgg aaactcctgc acaaggctcg gcgagatcgc gctccatgcc
    22321 gagttctccg tcgagggcac gctgatcgcc aacaatctcg tggacagggc cgcggccggg
    22381 atctccgtcg ccaacttcgc gaggggcgga aggctcgcgg tcatccaggg caatctcatt
    22441 cgcaacctct tcatgcgcaa ggaggccgac gcgcgcggtt tcggcatcgc cgtcgaggcc
    22501 gacagcgtgg tgacgggaaa tgtgatcgaa ggcgcgcccg cttacgggat catgatcggc
    22561 tggggaccgc acttgcgcga cgtgagcgtc accgacaatc tgatccgcga tgcgctgatc
    22621 ggcatcggcg tctcgatcga tccgtcggcg ggcaccgctc tcatcaccaa gaacctgatc
    22681 gcgggcacga aggagggggc gatccgcgcc atgagcgggc cgaccttgat cggccccgat
    22741 ctcgcgacgg cgagcgccga aaatttcggc aatgtcgcgg tctatgccaa cgtcgcgcgc
    22801 taggggatat cgacaaaggc attaggctga gccgccgggg agtgtccgct tgacccccga
    22861 tagcgggcgc ccggcgaggc cctcgaaaga gtccgctatg tgccagaagc cgtccttcct
    22921 aaagctggcg cgcctccaac cgaagtcgac cccatggcgg tgcttaagtg ttgttcgcga
    22981 atatgcgcta cacgacggtg cacagcggat ttccgcaata aggagcaact ccgatcaaaa
    23041 ttgagggatc tgccggggta aagcacgcgt ccgcttggtg ccaggagcgg tcatccgagg
    23101 ccgctcaggc cagagttgcc cggtcaaact ggaagaaagc caccacgttg ctcagcgagg
    23161 caagaccttt gtccgatcgc cagtggcacg atcgatgcgc acgattgcgg gaagcgccga
    23221 gtcactgaca accagcacgc cgtcaagaac ttcgacgatg ccccatggcc gcttgagcgc
    23281 agggccgatt ccgtttttcg gccggagacg atgtgcatct gctgtctgtt gggatcaatg
    23341 gcgtagacgg tgagtgtctt caaatcggtg acaaccaagt tcccctttcc gtccgctccg
    23401 attccgaccg gtgactgcaa cgtgacttcg cttcctggca aaacgagccg cagcatcatg
    23461 cgcttgccgt cgccggggtc gactgcgaag acggcagagc tgaccggatc gctcacatag
    23521 tagcggctct caagtgccgc gatgccgatg ggcacgccga acttcgggcc gcttccgaaa
    23581 tctgccccgg agacaatctt ccggtcgccg gtggcggtgt cgaccgcgat gacggcaccg
    23641 aggtcgggat cggtcgttac cgccatgtca tctgtgagcg ccaacgcaat tggatcctgg
    23701 atctccgggc caacaccccg ggcgggtccc gatatcacgg tacggttccc cgtccgccga
    23761 tcgacccgga tcatggcaat gttggtcgcg tccccaacga tcaggtcgcc ggtcgcagtg
    23821 atcgccaccg attccggcgt gatgaagaac atgccgtcgc cgtgcgcggc atcggaaacg
    23881 acccgtgcca cgccggtttg tgggtcgacc gcgatcacag cggcgcgttg cggatccact
    23941 gtcacgatct ccccgctgcc agtggcagcg atgcccatcg gatagaggag ggtcccgacc
    24001 gcgagagcgt cggctttgta ggtgatcgtg cggtcaccgt tggcgggatc gaccgcgagg
    24061 atggcgcgct gcccgaggtc cagaacaaac agcttccccc cgacaagcgc caggtcgacg
    24121 ggcatgacga atgcgggacc ggctcccgcc tccccgcccg agacttcggt gcggtccccg
    24181 cttgagggct cgacagacac aatgctcttg agggccgcgt cgacaaccag cagacggtct
    24241 tccggcgcca ggacgacccg agtagggctg tggaatccag gaccctcgcc ccgttctggc
    24301 ccggaaacga tctttcgatc gcccgtctcg aggtcgacgg ataggatcgc ttgcaatccg
    24361 cggtcgagga caaaaagccg acctgtggag gcagcgacca tgccgaacgg atcgacgaag
    24421 ggaggcccct tgcccactgt cggcgaggaa acgatcgagc ggtcgccact ctccatgtca
    24481 actcggtaga ccgagttcag cccgatgtcg aagaccacaa aggcggtgcc tccgaggcgt
    24541 gcgatcgcac gcgccgtcga gaactgcggg ccgtcgccgt tgtgctttcc tgagagttcg
    24601 gttcggtcgc ccgtccgtgg ctcgatccgg atgacggcac cttcgcccgg gtcgatcaca
    24661 tagtaggact tcgtatcggc aaccacggcg acaggggcat tgaaggaatt cccaccgcct
    24721 cgctggtccg acgagatcag ctggcaggct ccagtcgtcg gatcgacgac gacaacagcc
    24781 cgcagcccgc gatccgcgat gagaagctct ccgtcggagg tcgaggccat ccccattggg
    24841 gcgacgaaga tgctccttgc ctccgcgcat cctggttgag gctgggtcgc cgactcgccg
    24901 tggctgggaa ggagcgtcgg gcccgagacg caggtgacca ggaccgccaa cgcgatcccc
    24961 cgtggatcaa actccatagg tggcttcctt ttctgatccg gctgccggac gttgagaaga
    25021 atgcgagcac gcggcacgct gggcgagacg aacctgccat ttccgcttca gcccgtacat
    25081 ggcgtcgcca cctgggttcg gtttgcgcaa gagtccgctt agggtcatta gcggacgcga
    25141 tttgctaaac cattcgatgt ccgcgttggt cccaaaagtg gactgagcgc tagaaatcgg
    25201 cgttgcacgt taaggcactc ccgccttccg gcgcggcgcg gtcgtgatgg ccgcaagtcc
    25261 gtgcatcagc gctgaactcg ccacgacccg ccccacgcgc aagccgcgcc aattgaggag
    25321 ctcgccgtcg agcacttcgg caagcgcggt ccgtgcctct gggatcagcg ccgcgagtac
    25381 ctcggcgagc gcacgctcgg caccgcgttt cgcgccgtcg tccatcttga gcgcgatgcc
    25441 aaggccaagc tcgggcaacg ccgcgcaatg caccccctcg gcgccgccct tgacgaacag
    25501 ggccggcgcc aaggcacgca tagctatggt gtcgaagcgc ccctcgcccg ccacgagcac
    25561 gggaagcgca aagcaggcgc ggcgcagtcg ctccgcggcc cgtgcccgcg cggagccaag
    25621 gcccgtcccc gtcccaaatc gggcaaagcc ttgcgccaga gcggcgagcg gaagcgccca
    25681 ggtcggcacc gagcacccgt cgatcccaat cccatcgcgg ctcaaccgga ctccgcaagt
    25741 ctctgaaatg attcccgcga tcgtgacttg caggtcgtgg tccggatgct catagccgct
    25801 cggatcgagg ccgagatgga cgcatgcggc gagcattccc gcatgcttgc ccgagcaatt
    25861 gttgtggatc ggaagcggac ggccgccgcc acgcattacg tcgcgaaagg cgctgtcgct
    25921 gaccggccaa tgggccccgc aggcgagata gctttcgtcg agtcctgcct tgtcgagcaa
    25981 gcttgccacc gactcgatat gcacccgatc gccgctatgc gaggcgcaag cgaccgcaag
    26041 ctcggcatcg ccaagccgaa acgcatcggc ggcgccgctc tcgacgaggg ccaaggcttg
    26101 cagcgctttc accgcggagc gtggaaagac aggccgctcg acatcgccaa gggcaagaac
    26161 gaggccgccg gaagcatcgg cgatggcgat ggcgccggaa tggcggctct cgacgaccct
    26221 cccccgcatc acctcgacga gcacgggatt tgaggaagcg ctcacgctca tgccgcggcg
    26281 atgaggatgg gagcacggcg cgcgccccaa ttccccggcg cgggttcgaa caaaccgggg
    26341 catggcgcgc cgcaggagac gcagcgtccg ccctcgtcga gctgccatcc ggtgatcgtg
    26401 taccagtcgc ggccgatcac acgctcgccg caagacgcgc aataggtgct ctgacccgcc
    26461 tcgtcgtgca cgttgccgg
//
LOCUS       DASUQP010000059         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_164328,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000059 DASUQP010000000
VERSION     DASUQP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164328"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFY74_02650"
     CDS             <1..773
                     /locus_tag="VFY74_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5930639.1"
                     /translation="AVQGARALSGPLVSALSVAGFMLVWLVAAELAASRLLPGPVAVL
                     EYIYQESVHGDLLTELGITLWRVAASFAVAMLIGSVIGLAMGQMPALNRVLDPWLIVL
                     LNLPALVIIILAYVWFGLNEAAAIGAVALNKIPNVVVTLREGARALDTGLDEMARAYR
                     LSPLSKLRNVTLPQLQPYFAAASRTGLSLIWKIVLVVELLGRPNGVGFELHLNFQLFN
                     VTAILGYTLAFVAVMLAIEYLLVQPIERHSTRWRNKPV"
     gene            733..1485
                     /locus_tag="VFY74_02655"
     CDS             733..1485
                     /locus_tag="VFY74_02655"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5930640.1"
                     /translation="MNDIRPAGVTSRFEIRVEEKTHRSADGTRLVAIRNFVLDVAPES
                     MTVLMGPSGCGKTTLLRIVAGLDDRFTGTVGIHPEARIGLMFQEPRLLPWRTVRQNIE
                     LVAAPDFSAEDLDRLAHALGIGDMLPRFPQELSLGLARRVALARAFSTKPDLLLLDEP
                     FVSLDERTADRLRRLLLEVWQARPTTAILVTHNAREAILLADQLVLLAPRPTHVIAVE
                     TIAVPQRERDPKTIEAIHADLLRRYPANFATG"
     gene            complement(1876..>2431)
                     /locus_tag="VFY74_02660"
     CDS             complement(1876..>2431)
                     /locus_tag="VFY74_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5930641.1"
                     /translation="MVYDREMGSMRVLLTTPLPRWFLLVARLFAGVMVSLFQVYAFLL
                     VAYFCGVELPPLGYLAALPALILTGMMFGSLGMLLSSTIKQLENFAGIMNFVIFPMFF
                     ASSALYPLWKMREGSLWLYYLCLYNPFTWAVELIRFAFYLESNWQALGIVGACMLLFL
                     ALSVWAYDPSFGIQQRKVVAAASE"
BASE COUNT          459 a          794 c          716 g          462 t
ORIGIN      
        1 aggcggtgca aggggcgcgt gccttgagcg gaccgcttgt ttcggccctt tccgtcgcgg
       61 gcttcatgct tgtctggttg gtggcggcgg aattggcggc gagccggctt ctgcccggcc
      121 ccgtcgccgt gctcgaatac atctatcagg agagcgtaca tggcgacctc ctcaccgaac
      181 ttggcatcac gctgtggcgg gtcgcggcct ccttcgcggt ggcgatgctg atcggcagcg
      241 tgatcgggct cgccatgggc cagatgccgg cgctgaaccg ggttctcgac ccttggttga
      301 ttgtcctcct caatctgcct gcgctcgtta ttatcatcct cgcctatgtc tggtttgggt
      361 tgaacgaggc cgccgccatc ggcgcggtcg ctctcaataa gatccccaac gtcgtcgtga
      421 cgttgcgcga aggtgcccgc gcgctcgata ccggccttga cgagatggcg cgcgcctacc
      481 gtctgtcacc cttaagcaag ctacgtaacg tcacccttcc ccagcttcag ccttatttcg
      541 cggcagcctc acgcacgggc ctatcgctca tctggaagat cgtgctcgtc gtcgaactgc
      601 tcggccggcc gaacggcgtg ggcttcgagc ttcacctcaa tttccaactc ttcaacgtga
      661 cggctattct tggttacacg cttgcctttg tcgccgtcat gttggcgatc gaatatctct
      721 tggtgcagcc cattgaacga cattcgaccc gctggcgtaa caagccggtt tgaaatccgc
      781 gtcgaggaga agacccaccg cagtgccgac ggcacccggc tggtcgccat ccgcaatttc
      841 gtgctcgatg tcgcacccga aagcatgacg gtgctgatgg gcccgtcggg ctgcggcaag
      901 accacgctct tgcgcatcgt ggccggcctc gacgatcgct tcaccggcac ggtcggcatt
      961 caccccgagg cgcgcatcgg gctcatgttc caggagccgc gcctcttgcc gtggcgaacg
     1021 gtcaggcaga acatcgagct ggtcgcggca cccgatttct ccgcggagga tcttgatcgt
     1081 ctggctcacg ccctcggcat cggcgacatg ctgccgcgct tccctcaaga gctgtcgctc
     1141 gggctcgcgc gccgtgtggc gctcgcccgc gccttttcca ccaagcccga cctcctcctg
     1201 ctcgacgagc cgttcgtgtc gctcgacgag cggacggcgg accggttgcg gcgcctgctg
     1261 ctcgaagtgt ggcaggcgcg gcccaccacg gccattctcg tcacgcacaa tgcgcgcgag
     1321 gcgatcctgc ttgccgatca actggtcctg ctcgcgccac gccccactca tgtcatcgcc
     1381 gtcgagacca tcgccgtgcc gcaaagggag cgcgacccca agacaatcga agccatccac
     1441 gccgatctgt tgcgccgcta tcccgccaat ttcgccacag gctgagtcta agctcataag
     1501 cccgaatcag ctaagcttcc gctggctaga gccgatacat aacgtcgacc ccggactagg
     1561 gtcattccac taaactcagc tccgccctcc ggcggagctt tctgttaggc ctcgcaaaaa
     1621 aatgccgccg cctttgcagg cggcggcatc agttcaagac acccatgcaa agagaattag
     1681 ccgcactcag cttgcgctgc tgccggagct cgacatggcc agctcagctc tgctgtgccg
     1741 gcggggcttt gcgccgcggg tttatccgca gtttcgctgg ggtcacaacc tattgttaag
     1801 gctaagaaat cagcttccgc tcaagattta tcctgcgtct gactgaggcc cagtcgttac
     1861 cgtgacgtgc accgcttact cggaggcggc cgccaccacc ttgcgctgct ggatgccgaa
     1921 ggacggatcg taggcccaca ccgagagagc caggaacaga agcatgcaag cgccaacgat
     1981 cccgagcgcc tgccagttgc tctcgagata gaacgcgaaa cggatcagct cgacagccca
     2041 ggtgaaggga ttgtagaggc agaggtagta gagccacaga ctcccttccc gcatcttcca
     2101 cagcggatag agtgccgatg aggcgaaaaa catcgggaag atcacaaagt tcatgatacc
     2161 ggcgaagttc tcgagctgct tgatcgttga cgacagcagc atgccgagcg agccgaacat
     2221 catgccggtc aggatcagcg ccgggagcgc cgcgagatag ccgagcggcg gcagctcgac
     2281 gccgcagaaa taggcgacaa gaagaaaggc ataaacttgg aacagcgaga ccatcacgcc
     2341 ggcaaaaagg cgtgccacaa gcaggaacca gcgcgggaga ggcgtggtga gcagcacccg
     2401 catcgagccc atctcgcggt cgtaaaccat c
//
LOCUS       DASUQP010000060        13691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_573336,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000060 DASUQP010000000
VERSION     DASUQP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573336"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..957
                     /gene="argB"
                     /locus_tag="VFY74_02665"
     CDS             31..957
                     /gene="argB"
                     /locus_tag="VFY74_02665"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5930642.1"
                     /translation="MADVIASVHAKAKVLSEALPYMQRYDRKTVVVKYGGHAMGDPVL
                     AADFARDIVLIKQAGVNPVVVHGGGPQIGLLLDRLNIRSEFKGGLRVTDRATVEVVEM
                     VLAGSVNKEIVTAINSQGGKAVGISGKDANLMRAKRLERRWRDPSSNIEQIADLGFVG
                     DPAAVDPHIIDVIIQSDLIPVVAPIGVGPDGETLNINADTFAAALASALKAKRLLLLT
                     DVEGVLDRNGNLIKSLTTAEAQSLIDDGTISGGMIPKIESCIDVIAEGVEGVVIINGK
                     VPHAVLLELFTEHGAGTLIERRRRRILARGAQ"
     gene            954..1718
                     /locus_tag="VFY74_02670"
     CDS             954..1718
                     /locus_tag="VFY74_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX5930643.1"
                     /translation="MTERHGAVRGFDHVEAWVFDLDNTLYPADCNLFAQIDRRMGEFI
                     ANAFGIPLAEAQSLREAYYYEHGTTLAGLVRLHDVPPDDFLDYVHDIDLGAVAPCPEL
                     AAALTALPGRKFIFTNGSRKHAEAVVERLGVSGQFADIFDIHALGYVHPKPTREAYER
                     FVGAHEVMPRKAAMFDDLPHNLETAHAIGMTTVLVHGLTEHPEHQAMTGWTELPAHIH
                     HRTEALPSFLAAIGATLTSDDAGRSLPQAQAQFCLL"
     gene            1728..2570
                     /gene="dapD"
                     /locus_tag="VFY74_02675"
     CDS             1728..2570
                     /gene="dapD"
                     /locus_tag="VFY74_02675"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX5930644.1"
                     /translation="MTHDHLKTFIDSAYEHAVEITPETKGVVRDAVDEVLDLLDRGEL
                     RVAEKRDGSWIVHQWLKKAILLSFRLSEPHKMDGGPDGGAWWDKIPTKFAKWKTKDFK
                     EAGFRAVPGAVVRRSAFIAPGVVLMPSFVNLGARVESGTMIDTWATVGSCAQVGRNVH
                     LSGGAGIGGVLEPIQANPVIVEDNCFIGARAEVAEGVIVGEGSVLSMGVYIGQSTKII
                     DRATGVVHQGRVPPFSVVVSGTMPGKPLPDGTPGPALYCAVIVKTVDERTRAKTSINE
                     LLRD"
     gene            2616..3056
                     /locus_tag="VFY74_02680"
     CDS             2616..3056
                     /locus_tag="VFY74_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX5930645.1"
                     /translation="MDVDFASLLLSRDGRINRAKFWIGAVGFLVILIIVDVIFGSSIF
                     GRILKFLIAIALFYPAYAVAAKRFQDRNKPGETALYGLVPVFVAILLDCFGLTGDATG
                     ATAIGWICRLIYLGVGLWFAIELGTLKGTPGPNRFGGDPLAAVR"
     gene            3121..4311
                     /gene="dapE"
                     /locus_tag="VFY74_02685"
     CDS             3121..4311
                     /gene="dapE"
                     /locus_tag="VFY74_02685"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX5930646.1"
                     /translation="MTALGDKALHLAQNLIRCESVTPADAGALDVLIGTLKPARFTFW
                     RLNFKDEGSPPVDNLYARIGDAPPHLCFAGHVDVVPPGDSALWNHPPFAAELSDGILY
                     GRGASDMKGAIACFTAAALDYVKSRGRELDGSISLLITGDEEGPAVNGTCKVLAWMHE
                     RGERPDHCLVGEPTNAKRLGESIKIGRRGSLNGILKVTGVQGHVAYPHLANNPVKGLV
                     KILTRLYDTPLDYGSAHFSPSNLEVTSVDVGNATTNVIPAFAEARFNVRFNDRHTADS
                     LMEQLGEQAKLALSGTDLSLSLEFEPPSVSFLTEPGPLDALLSEAVNEVTGLTPSLST
                     DGGTSDARFIKEYCPVVEFGLLTASIHKADENVSVADLEQLTAIYRRFIALYFETFGG
                     VDAG"
     gene            4301..5137
                     /gene="murI"
                     /locus_tag="VFY74_02690"
     CDS             4301..5137
                     /gene="murI"
                     /locus_tag="VFY74_02690"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX5930647.1"
                     /translation="MPGDPRPIGVFDSGMGGLTVLKALAARLPNERFVYLGDTARLPY
                     GTKSAETVQAYALQATRLLLDQDVKMLVIACNTASAVAVELLQQACAPIPVIGVIEPG
                     ARAGVAATSNRRIAVIATEGTVRGGAYACAIRRLMSDAEVVQQPCQVFVALAEEGWAD
                     APATRAAAEHYLATLFESASPPDTLVLGCTHFPVLADAIRAVIGGRVALVDSAETTAG
                     AVAEALSGAGLASAAEAREGAPVRFFATDSPERFARVGAIFLGRAIDPAAVTLVDLRT
                     FA"
     gene            complement(5134..6765)
                     /gene="murJ"
                     /locus_tag="VFY74_02695"
     CDS             complement(5134..6765)
                     /gene="murJ"
                     /locus_tag="VFY74_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5930648.1"
                     /translation="MIRRIATVGGLTLVSRLTGFVRDVVMAAILGAGPIADAFFVAFR
                     LPNHFRAIFAEGAFAASFVPAYARTLEEAGLPAAKLFADQIAAALIAINLLLLGLALL
                     FTPWVVSVLAPGLVDDPQRFDLAVALTRITFPYLALVSIETLFAGVLNANNRFAAAAG
                     APILLNLSVVATLLLAPLFSDAGHAAAWGVLIAGVAQVLLVGIDAESRGYGVRLRIPR
                     MSEPTRRFLKALGPAIIGAGGVQLALFADTLIASFLPTGALSALYYADRINQLPIGVV
                     GIAVGTVLLPEMARRLAAGDERGAASAQARGVQLALLLTVPCAAAAVTIPDLIMRALF
                     ARGAFTASDAMAAGATLAAYSVGLLPFVLMRSFTAPFYARGDTATPVKAALLAAAINI
                     ALKVALMGPLAQVGLALATSVGAWINLSLLAFYARRQGFAVSGAAIGKPVAKLIAAGF
                     VLAAALLAGAYGLERALSDLALFRDETELAILVVLGAVVYGLAVLLLLGRPWLRTLLR
                     EVTVAADTKAPADLEQTDPTDDSAVLPDNEPPPKA"
     gene            complement(7124..7861)
                     /gene="truA"
                     /locus_tag="VFY74_02700"
     CDS             complement(7124..7861)
                     /gene="truA"
                     /locus_tag="VFY74_02700"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX5930649.1"
                     /translation="MPRYKLTLEYDGTPFVGWQVQENGPSVQARLAEAIKAFSGEEGV
                     PRGAGRTDAGVHALGQVAHIDLAKDWPPWKVRDALNAQLRPDPISVLACEPAAPDFDA
                     RFSATARHYLYRIVNRRGPLALDRDRAWQVPHALDAGAMHEAAQVLIGNHDFSTFRSA
                     ECQASSPVKTLDRLAVARNGEEIRIEAAARSFLHNQVRSMVGSLKLVGEGKWTAHDLR
                     DALEARMRGACGPVAPPCGLYLVQVDY"
     gene            complement(7865..8815)
                     /gene="fmt"
                     /locus_tag="VFY74_02705"
     CDS             complement(7865..8815)
                     /gene="fmt"
                     /locus_tag="VFY74_02705"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5930650.1"
                     /translation="MQTAWEPMMRIVFMGTPDFAVPTLRAIRDAGHEVAAVYTQPPRA
                     AGRGMTPRKSPVQHAAEQAGLPVRTPERLKGREEEERFRALEPDAAVVVAYGLILPKP
                     ILDGTRHGVFNVHASLLPRWRGAAPINRAIMAGDIASGVSIMRLAEGLDAGPVCLVGR
                     VKIGPEMTAGELHDALAPLGAQLMVKALAELEQGGLRCRAQHDEAATYAPKLDPREMR
                     INWRLKARDVQNRIRGLSPHPGAWFEVGIGGKRERIRVLRAALAKGSGPAGTFLDDKL
                     TIACGEGAVRLTEVQRAGRKPMPAADFLHGVKLKAGIVLT"
     gene            complement(8877..9059)
                     /locus_tag="VFY74_02710"
     CDS             complement(8877..9059)
                     /locus_tag="VFY74_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930651.1"
                     /translation="MSVEFGKRESLGTRIGKTAPDLSRLFLGELDRAFVMLLHHPDDL
                     GQVGLTFRRPGQNAIE"
     gene            9080..9364
                     /locus_tag="VFY74_02715"
     CDS             9080..9364
                     /locus_tag="VFY74_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5930652.1"
                     /translation="MKVVLHHSALADLDDLHAWISKQNPSAATAMVARIRDRISFLEI
                     DSLAQMGRPGLVPGTRELIEYPYIIVYRVNDRLREVNVLAIFHGAQSRAR"
     gene            complement(9425..9667)
                     /locus_tag="VFY74_02720"
     CDS             complement(9425..9667)
                     /locus_tag="VFY74_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930653.1"
                     /translation="MSHLLIRNINLKLKRRLAASARRSGRSLSEEAKALIDCALAETK
                     PARGLGTALVERFRVIGPVDLRDRAVEAPRRPPYLE"
     gene            complement(9725..9967)
                     /locus_tag="VFY74_02725"
     CDS             complement(9725..9967)
                     /locus_tag="VFY74_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930654.1"
                     /translation="MTLLTLSIALELAVAIIAAVAASKGRPYLYGLAFTFALYALYDA
                     GQLLGSNVEKGVLSVLHLLASASALYAVWGLYREES"
     gene            complement(9970..10485)
                     /gene="def"
                     /locus_tag="VFY74_02730"
     CDS             complement(9970..10485)
                     /gene="def"
                     /locus_tag="VFY74_02730"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003583874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5930655.1"
                     /translation="MAIKSILTLPDKLLRKQSKPVERVDAELRRLIDDMFATMYAAPG
                     IGLAAPQVGVSRRLIVMDPAKDDAPKTPLVMVNPEILDRSEELRMHEEGCLSIPDFLA
                     EIERPAKTRVAYIDSEGKPQERELEGIWSTLVQHEVDHLNGILFIDYLSRLKRDMVVR
                     KFTKQKRGEAL"
     gene            11117..12034
                     /locus_tag="VFY74_02735"
     CDS             11117..12034
                     /locus_tag="VFY74_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5930656.1"
                     /translation="MAATEACHRDLAATGADLSAYNSSESAADFADLRKVLGYAAWNV
                     YGTSYGSYLAQTLMRDHPEGIRSVVLDVRDPATGEDIEVVIDGGAIVDWLRNQNYAVP
                     LLRAVPDRIDGLAAGRPDAIAAIATDRASRAPPPGPDVPALGYGLAFGVSCRESYPFA
                     TPEDLAAAGREAFPHYPASIQDEGVGGWAYFNEDCGEVWKVPAAPEAMHQPVASSIPT
                     LLISGSFDTLTSLAGAKAAAAKLSNATIISIPGVGHFVSPASPCAQAVIVSFLTPPTR
                     RASALSRRRASPRRRRPKRQGILNVPVLV"
     gene            12065..13303
                     /gene="rmuC"
                     /locus_tag="VFY74_02740"
     CDS             12065..13303
                     /gene="rmuC"
                     /locus_tag="VFY74_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX5930657.1"
                     /translation="MVFEPIRIGNVAIDPVIAFFVLAGLAGFALVLLAVALFVHMSRR
                     RREGEAQAAELAELKGRLQSFAEISVARQGDIARAVNERLDRMTHRVGTDLNETSRKT
                     HESIAKLNERLAVIDTAQRNLTDLSNNMVSLQEILANKQARGAFGQLRMETIVKDGLP
                     KGAYTFQATLSNGKRPDCLLHMPNTKASVVIDAKFPLEGFEAFRAARREEEKKEAARR
                     VRFDVGRHVDSMAERYFIPGETQDTAILFVPSEAIYVDLCEHFSDLVQRAHRARIVIC
                     APNMLMLAVQTMQAILKDVQMREQAHLIQREVARLMDDMGRFRERVLDLQRHFGQTNQ
                     DIEKILTSSEKIAERGRKIEMLEFEDGAPAAIEQVNTSPSPGAAGPRPDAPPSPARGE
                     GRERKRNGGSARLPGLLPGE"
BASE COUNT         2439 a         4511 c         4483 g         2258 t
ORIGIN      
        1 gcgctgaaaa cccgcgcggc ggcccagagc gtggccgacg tcatcgcctc ggtccatgcc
       61 aaggccaagg tgctgtcgga ggcgctgccc tacatgcagc gctacgaccg caagaccgtg
      121 gtggtgaaat atggcggcca cgccatgggc gatccggtgc tcgccgccga ttttgcccgc
      181 gacatcgtgc tcatcaagca ggccggcgtg aacccggtcg tggtgcatgg cggcggcccg
      241 cagatcggct tgctgctcga ccggctgaac atccgatcgg agttcaaggg gggcttgcgc
      301 gtcaccgaca gggcgacggt cgaagtggtc gagatggtgc tcgcaggctc cgtcaacaag
      361 gagatcgtga ccgccatcaa cagccagggc ggcaaggcgg tcggcatctc cggcaaggac
      421 gccaacctga tgcgggcgaa gcggctggag cggcgctggc gcgatccttc ctccaatatc
      481 gagcagatcg ccgatctcgg cttcgtcggc gatcccgccg ccgtcgatcc gcacatcatc
      541 gacgtgatca tccagtccga tttgattccg gtcgtggccc cgatcggggt cgggcccgac
      601 ggcgagacgc tcaacatcaa cgccgacacc ttcgctgcgg cgctcgcctc ggccctgaag
      661 gcgaaacgcc tcctgcttct gaccgacgtc gagggcgtgc tcgacaggaa cggcaacctg
      721 atcaagtcgc tgaccacggc ggaggcgcaa agcctgatcg acgacggcac gatatccggc
      781 ggcatgatcc cgaagatcga gagctgcatc gacgtcatcg ccgaaggcgt cgagggcgtt
      841 gtcatcatca acggaaaggt gccgcatgcg gtgctgctcg aactcttcac cgaacatggc
      901 gccggcacgc tgatcgagcg gcgccggcgc aggatcctgg cgaggggagc acaatgacgg
      961 aacggcacgg ggcggtgcgc ggcttcgatc acgtcgaggc ctgggtgttc gatctcgaca
     1021 acacgctcta cccggccgac tgcaatctgt tcgcgcagat cgaccggcgc atgggagagt
     1081 tcatcgccaa cgcgttcggc atcccgctcg ctgaggcgca gagcttgcgc gaagcctatt
     1141 actacgagca cggcaccacg ctcgcagggc tcgtgcgcct gcacgacgtg ccgcccgacg
     1201 atttcctgga ttatgtgcac gacatcgatc tcggcgcggt cgccccctgc cccgagcttg
     1261 cggcggcgct cacggcactc ccggggcgca aattcatctt caccaacggc tcgcgcaagc
     1321 atgccgaggc cgttgtcgag cggctcggcg tgagcggtca attcgcggat atcttcgaca
     1381 tccacgcgct cggctatgtc cacccgaagc cgacgcgcga ggcctatgag cgcttcgtcg
     1441 gcgcccatga ggtgatgccg cgcaaggcag ccatgttcga cgacctgccg cacaatctgg
     1501 agaccgcgca tgccatcggc atgaccacgg tgttggtgca cgggctgacc gagcaccccg
     1561 agcatcaggc gatgacgggc tggaccgaac tcccggccca tattcatcat cgcaccgagg
     1621 cgcttccctc cttccttgcg gcgatcggcg ccacgctcac gtcggatgat gccgggcgct
     1681 ctctcccaca agcccaagcg caattctgcc tcctctgacg ggccgccatg actcacgacc
     1741 atctcaagac cttcatcgac agcgcctacg aacatgccgt cgagatcacg ccagagacca
     1801 agggcgtggt gcgtgacgcc gttgacgaag tgctcgatct gctcgacagg ggcgagcttc
     1861 gcgtggcgga gaaacgcgac ggcagctgga tcgtgcatca atggctcaag aaggcgatcc
     1921 ttctttcctt ccgcctcagc gagccgcaca agatggatgg cggccctgac ggcggcgcct
     1981 ggtgggacaa gatcccgacc aaattcgcca agtggaagac caaggacttc aaggaggcgg
     2041 gcttccgcgc cgtgccggga gcggtcgtgc gccgctctgc ctttatcgcg cccggcgtgg
     2101 tgctgatgcc ctctttcgtc aatctcggcg cgcgggtcga gtccggcacc atgatcgaca
     2161 cctgggcgac ggtcgggtcc tgcgcgcaag tgggccgcaa cgtgcatctg tcgggtggcg
     2221 ccggcatcgg cggcgtgctc gagccgatcc aggccaatcc ggtgatcgtc gaggacaatt
     2281 gcttcatcgg cgcccgcgcc gaggtcgccg aaggggtgat cgtcggcgaa gggtcggtgc
     2341 tgtcgatggg cgtctatatc gggcaatcga ccaagatcat cgatcgcgcc acgggcgtcg
     2401 tgcatcaggg ccgcgtgccc ccattttcgg tggtggtctc ggggaccatg cccgggaagc
     2461 cgctgccgga cggaacgccg gggcccgcgc tctattgcgc cgtcatcgtc aagacggtcg
     2521 acgagcgcac ccgcgccaaa acctccatca acgagctgct cagggattag ggcgagcgct
     2581 aatccatgcc gggcgaaggg gaataggcgg ggacgatgga cgtggatttc gcgtctctgc
     2641 tcttgtcgcg cgacggccgc atcaaccggg ccaagttctg gatcggcgcc gtcggcttcc
     2701 tcgtcatctt gatcattgtc gacgtcattt tcggatcgtc gatcttcggg cggatcttga
     2761 aattcctcat cgcgatcgcg ctcttctacc cggcctacgc ggtagcagcc aaacgcttcc
     2821 aggatcgcaa caagcccgga gagactgcgc tctacggatt ggttcccgtc ttcgtcgcaa
     2881 tcctgctcga ctgtttcggc ctgaccggcg atgcgaccgg ggcaaccgca atcgggtgga
     2941 tctgccgcct gatctatctc ggcgtcgggc tctggtttgc gatcgagctc ggcacgctca
     3001 agggcacgcc ggggcccaat cgctttggcg gcgatcccct ggccgccgtg cgctaggacc
     3061 ggcttgccat gcggctgggc agcggtcatg ttgctcggcc tatgaccact tgctcagcca
     3121 atgaccgcac ttggcgataa agccctccat ctcgcgcaga acctgatccg ctgcgagagc
     3181 gtgacgccgg cagacgcggg cgcgctcgac gttctcatcg gcacgctcaa gcccgctcgc
     3241 ttcaccttct ggcgcctcaa cttcaaggac gaggggtcgc cgccagtcga caatctctac
     3301 gcacgcatcg gcgacgcgcc gccgcatctt tgcttcgcag gccacgtcga cgtcgtcccg
     3361 ccgggggatt cggcgctttg gaaccatccg cccttcgccg ccgagctctc cgacggcatc
     3421 ctctacggca gaggcgcgtc ggacatgaag ggtgcgattg cgtgtttcac cgcagcggca
     3481 ctcgattatg tcaagtcgcg cggccgcgag ctcgacggat cgatcagcct tctcatcacc
     3541 ggcgatgagg aaggaccggc ggtcaacggc acgtgcaaag tgctcgcctg gatgcacgag
     3601 cggggcgagc ggcccgatca ttgcctggtg ggcgagccca ccaacgccaa gcgtctcggc
     3661 gagtcgatca agatcggccg gcgcggcagt ctcaacggaa tcctgaaggt gacgggcgtg
     3721 caaggccatg tcgcctatcc gcatctggcc aataatcccg tcaaaggctt ggtgaagatc
     3781 ctcacccgtc tctacgatac gccgctcgat tacggcagcg cgcatttctc gccgtcgaat
     3841 ctcgaagtga ccagcgtcga tgtcggcaac gccacaacga atgtgattcc ggcatttgcc
     3901 gaggcgcgat tcaacgtgcg cttcaacgat cggcacaccg cggattcgct gatggagcag
     3961 ctcggcgagc aggcgaaact cgctctgtcg ggcaccgacc tctccttgag cctcgaattc
     4021 gagccgccga gcgtctcatt cctcacggag cccggcccgc tcgacgcgct tctgagcgag
     4081 gccgtcaacg aagtcacggg tctcacgccc tcgctctcga ccgacggcgg gacctccgat
     4141 gcccgcttca tcaaggaata ttgcccggtc gtcgagttcg gactgttgac cgcgtcgatc
     4201 cacaaggccg acgagaacgt ttctgtcgcc gacctcgagc aactgaccgc gatctaccgc
     4261 cgcttcatcg cgctctattt cgagaccttc gggggcgtcg atgccgggtg acccccggcc
     4321 gatcggggtg ttcgactccg gcatgggcgg gctcacagta ttgaaggcgc tcgcggcgcg
     4381 cctccccaac gagcggttcg tgtacctcgg cgacaccgcg cggctgcctt acggcaccaa
     4441 gagcgcggag acggtgcaag cctatgcgct ccaggcgacg cggctcctgc tcgaccagga
     4501 cgtgaagatg ctggtgatcg cctgcaacac ggcctcggcc gtggcggtcg aactcctgca
     4561 acaggcttgc gcgccgatcc cggtgatcgg cgtgatcgag ccgggcgcgc gcgcaggggt
     4621 cgcggccacg tcgaatcgcc gcatcgccgt catcgcgacg gaaggcacgg tcaggggcgg
     4681 tgcttatgcc tgcgcgatcc ggcgcctgat gagcgatgcc gaagtggtgc agcaaccttg
     4741 ccaggtgttc gtggcgctgg ccgaggaggg ctgggccgat gcgcccgcca cgcgcgcggc
     4801 ggcagagcat tatctcgcaa cgctgttcga gagcgcctcg ccgccggaca cgctcgtcct
     4861 cggctgcacc cactttccag tcttggcaga cgccatcagg gcggtcatcg gcggccgcgt
     4921 tgcgctcgtg gactcagccg agaccacggc cggcgccgtc gcggaggcac ttagcggcgc
     4981 aggactagcc agcgcggccg aagcgcgcga gggggcgccc gtgcgattct tcgccaccga
     5041 ttcgcccgag cgtttcgcgc gcgtcggcgc gatctttttg ggacgcgcga tcgatcccgc
     5101 tgcggtcacg ctcgtcgatc tcagaacctt cgcttaagcc ttcggaggcg gctcgttgtc
     5161 cggcagcacg gcggaatcgt cggtcgggtc ggtctgctcg agatcggccg gcgctttcgt
     5221 gtcggcggcg acggtcactt cgcgcaacag ggttctgagc cagggacgac cgagcagcaa
     5281 aaggaccgcg agcccataga ccacggcacc aagcacgacg agaatcgcaa gctccgtctc
     5341 gtcgcggaac aaggccaaat ccgaaagcgc gcgctcaagt ccgtaggcgc cggccagtaa
     5401 tgccgccgcg agcacgaaac ccgccgcgat cagcttcgcc acgggcttgc cgattgccgc
     5461 gcccgatacg gcaaacccct ggcggcgggc gtagaaggca agaaggctga gattgatcca
     5521 ggcgccgacg ctcgtggcaa gcgcgagccc gacttgcgcg agcgggccca tcaacgccac
     5581 cttcaaggcg atgttgatgg cggcggccaa gagcgccgcc ttgaccggcg ttgccgtgtc
     5641 gccgcgcgcg tagaaggggg cggtgaagct ccgcatcagc acgaagggaa ggagcccgac
     5701 cgaataggcg gcaagcgttg cgcctgcggc catcgcgtca gaggcggtga aggcgccacg
     5761 ggcgaagagc gcgcgcatga tgagatcggg gatggtgacg gccgcggcgg cgcaagggac
     5821 ggtgaggagg agcgcgagct gaacgccgcg ggcctgggcc gaggccgcgc ccctttcgtc
     5881 gcccgcagcg agccggcgcg ccatctctgg aagcagcacg gtgccgacgg cgatgccgac
     5941 aacgccgatc gggagctggt tgatgcggtc ggcgtaatag agcgcggaca gcgcgccggt
     6001 cgggagaaac gaggcgatca gcgtgtcggc gaaaagcgca agctgcacgc cgcccgcgcc
     6061 gatgatcgcc ggaccgagcg ccttgaggaa gcgccgcgtc ggctccgaca tccgggggat
     6121 gcgcaagcgc acgccatatc cgcgactctc ggcatcgatg ccgacgagga gcacctgggc
     6181 cacgcccgcg atcaggacgc cccaggcggc cgcatggccg gcgtccgaga agagaggcgc
     6241 gagcagaagc gtcgcgacca ctgacagatt gagaaggatg ggcgccccgg cggccgcggc
     6301 gaagcggtta ttggcattga ggacgcccgc gaacagcgtc tcgatcgaca cgagcgcaag
     6361 gtagggaaag gtgatgcggg tgagcgccac ggcgaggtcg aagcgctgcg gatcatcgac
     6421 gagaccgggc gcgagcacgc tcacgaccca tggcgtgaac aaaagcgcca atcccagcag
     6481 caagagattg atggcgatca gcgcggctgc gatctggtcg gcgaagagtt ttgccgcggg
     6541 caaccccgcc tcttcgagcg tgcgcgcgta ggccggcacg aaggacgcgg cgaacgcgcc
     6601 ttcggcgaag atggcgcgga aatggttggg aaggcggaaa gcgacgaaga aggcgtccgc
     6661 gatcggcccc gcgccgagga tcgccgccat gaccacgtcg cgaacaaaac cggtgaggcg
     6721 cgagacgagc gtgagcccgc ccaccgtggc gatgcgcctg atcatgctct ctcgcgctcg
     6781 gtcaagagtc cctcccgttc gcgggctccc gttcgcggca gagacgtata tgaatttcag
     6841 cgccttgacc acacatgcgc tctcccctcg cgggagaggg cttagacatc gcggagaact
     6901 gtttcccctc tccccttgtg ggagagggct tcacgccgcg atcttgcgcg gcgaagcctg
     6961 cgcaagtcgc atggcgggaa gggtgagggt tttttttgga cgccgcttcg cggcttaccc
     7021 ctcaccgcgc gccagatagc gcgcgtgctc tcccacaagg ggagagcggg gagcgcgttg
     7081 ctgtgcttcg acaaaatatc cggattccgt tgcgtgctgc cgctcaatag tcaacctgga
     7141 ccaagtaaag gccgcagggc ggcgcgaccg ggccgcaggc gccgcgcatg cgcgcctcga
     7201 gcgcgtcgcg caagtcatgc gcggtccatt tgccctcgcc gacgagcttg agcgagccga
     7261 ccatcgagcg cacctgattg tgcaggaacg agcgcgcggc tgcctcgatc ctgatctcct
     7321 cgccatttcg cgcgaccgcg agcctgtcca gcgtcttgac cggagatgac gcctggcact
     7381 cggcagagcg gaaggtgctg aagtcgtgat tcccgatgag gacttgcgcc gcctcgtgca
     7441 tggcgccggc gtcgagcgcg tgcggaacct gccaggcgcg gtcgcggtcg agcgcaaggg
     7501 ggccgcgccg gttgacgatg cgatagagat agtggcgcgc ggtggccgag aagcgcgcat
     7561 cgaaatcggg ggcggcaggc tcgcaggcaa gaacgctgat cgggtccggc ctgagctgtg
     7621 cattcagcgc atcgcgcact ttccacggcg gccagtcctt ggcgaggtcg atatgcgcga
     7681 cttgcccaag cgcatgcacg ccggcgtcgg ttcgccccgc cccgcgcggg acgccctctt
     7741 cgccggagaa agccttgatg gcctcggcga ggcgcgcctg caccgaaggc ccgttctcct
     7801 gcacctgcca tccgacgaag ggcgtgccgt catattcgag cgtgagcttg tagcggggca
     7861 tggcttaagt aagcacgatc ccggccttga gcttgacgcc gtgcaggaaa tcggccgcgg
     7921 gcatcggctt acgtccggcg cgctgcactt ccgtgagccg caccgcgccc tcgccgcagg
     7981 cgatcgtcag cttgtcgtcg agaaacgttc ccgcaggacc ggaccccttg gcgagcgccg
     8041 cccgcaacac cctgatgcgc tcgcgcttgc cgccaatccc gacctcgaac cacgcgccgg
     8101 gatgcggcga cagaccgcgg atgcggttct gcacgtcgcg cgccttgagc cgccaattga
     8161 tgcgcatctc gcgcggatcg agttttgggg cgtaggtcgc cgcctcgtcg tgctgggcac
     8221 ggcaacggag accgccctgc tcaagctcgg caagcgcctt caccatcagt tgggcgccga
     8281 ggggggcgag cgcatcgtgc aattcgcccg ccgtcatctc tggaccgatc ttgacgcggc
     8341 ctacgaggca gaccgggccg gcgtcgagcc cttcggcgag gcgcatgatc gacaccccgc
     8401 tcgcgatgtc gcccgccatg atcgcgcgat tgatgggagc ggcaccgcgc cagcgcggga
     8461 gcagcgaagc atgaacattg aagaccccgt gacgggtgcc gtcgagaatg ggtttcggca
     8521 agatcaggcc ataggccacg accacggcgg catcgggctc gagcgcacga aagcgctcct
     8581 cttcctcgcg gcctttgaga cgctcgggcg tgcgcacggg aaggccggct tgctcggcgg
     8641 cgtgttgcac cggcgatttg cgtggcgtca tgccgcggcc ggcagcgcgc ggcggctgag
     8701 tgtaaaccgc cgcgacctca tgccccgcat cgcgaatggc gcgaagcgtc ggcacggcaa
     8761 agtccggcgt tcccatgaac acgatgcgca tcatcggctc ccaagcggtt tgcatattgg
     8821 caccgacaaa ccagcagagt taaactaatg caatgacaag agaccaagtt aaggaattac
     8881 tcgatcgcgt tctgacctgg ccgcctgaac gtcaggccga cctggccgag gtcgtcagga
     8941 tgatggagca gcatgacaaa agcgcgatcc agctcaccga ggaacaggcg gctgaggtca
     9001 ggcgccgtct tgccgatgcg cgtcccaaga ctctcacgct tgccgaactc aacgctcatc
     9061 tacgccaacg ctatggcgta tgaaggtcgt ccttcaccat tcagcgcttg cggacctcga
     9121 tgatctccac gcttggatca gcaagcagaa cccatcggca gccactgcga tggtcgcacg
     9181 gatacgagac cgaatctcat ttttggagat cgattcgctg gcgcaaatgg gccgaccagg
     9241 actcgttcca ggcacacggg aattgatcga gtatccctac atcatcgttt accgggtcaa
     9301 cgatcgtctg cgggaagtca acgtgctcgc gatcttccac ggcgctcaaa gccgcgcccg
     9361 atgatgcgca tcatcggcgg cgtgttcgcc gcttggtctc cgacgacgtt cgtaccaaga
     9421 acgatcattc caaatagggc ggccttcgcg gcgcttcgac cgcccggtct cgaaggtcca
     9481 cgggcccaat cacgcggaaa cgttccacga gcgcggtccc gagcccgcgt gccggcttgg
     9541 tttcggccag cgcgcagtcg atcagggcct tggcttcctc ggagaggcta cgtccgctcc
     9601 ttcgagcgct ggccgcgagg cgccgcttca gcttgagatt gatattgcgg atcaacagat
     9661 gagacatttg atcaatgata tcgagcgggc gcgagtttgc aacgcgatcg cgacaaacgg
     9721 cgaactacga ttcttcgcga tagaggcccc acacggcata gagcgcgctc gcgctcgcaa
     9781 gcagatgcag caccgagagc acgcccttct cgacgttcga tccaagcagc tggccggcgt
     9841 cgtagagcgc gtaaagggcg aaggtgaagg caagaccata gagataaggg cggcctttgc
     9901 tcgccgcgac cgcggcgatg attgcgacag ccaattcgag cgcgatggaa agcgtgagca
     9961 gcgtcatgtc tagagcgcct cgccgcgttt ttgcttggtg aactttcgca caaccatatc
    10021 gcgcttcagc cgcgacagat aatcgatgaa caggatgccg ttcagatgat cgacctcgtg
    10081 ctgcacgaga gtcgaccaga tgccttcgag ctccctctcc tgaggcttgc cctcgctgtc
    10141 gatataggca acgcgggtct tggccggccg ctcgatctcc gcgaggaaat cgggaatcga
    10201 aaggcatccc tcctcatgca tgcgaagctc ctcggagcgg tcgaggatct cggggttgac
    10261 catgacgagc ggggtcttcg gcgcatcgtc cttagccggg tccatgacga tcaggcggcg
    10321 ggagacgccg acctgcggcg ccgcgagccc gatgcccggc gccgcgtaca tggtggcgaa
    10381 catgtcgtcg atgagccggc gaagctcggc atcgacgcgc tcgaccggct ttgactgctt
    10441 gcgcagaagc ttgtcgggaa gggtgagaat ggatttgata gccatttgga gcgctgaaat
    10501 aaggacacga tgggcgccgg tcaatgcgcg gctggtcccc atcgaaggct ggaggcgccc
    10561 ctttcaagcg gtttggtccc cctgcggtac gtgggcgcgt atagtgggag accggatagg
    10621 ccaacctatc cttacggagg gctaagccta tgtcttacgg tgcccgcgtc gctctcttta
    10681 ttctcttggc ggtgccgggc ccgcttgcgc aagctgaggc agcgcggccg gtgccgcgct
    10741 tcgaggaggc gccatgcccg aaactgccgg gggccgaagc gctggccaag gcgagctgtg
    10801 gctacctcgt cgtaccggaa aaccgtggcc ggccgactgg tcgcaccatc aggctcatgg
    10861 tcgcgacata ccccgcccgg tcgccggaga agctgtccga tcccgtcgtc tatctcgccg
    10921 gcgggccggg cgacattgta cccctggagg tcaacgggtt catagccgcc gatttcatcc
    10981 gcgaccggga catcgtggcg atgagccagc gcggcaccat gttctcggag cccgccctta
    11041 cctgcgcgcc catcgacgac ttcgccagag agctcctcgg cctccgcttc tattcagaag
    11101 caaccaagcg cgctcattgg cggcgacgga agcctgtcac cgggacctcg ccgccaccgg
    11161 cgccgacctc agcgcctaca attcgagcga aagtgccgcc gatttcgccg acctccgcaa
    11221 ggtgctcggc tatgccgcat ggaatgtcta cgggacgtca tacggcagct acctggcgca
    11281 gacgctcatg cgcgaccacc ccgagggcat ccgcagcgtc gtcctcgacg tccgcgaccc
    11341 ggcgaccggt gaagacatcg aagtcgtgat cgatggcggg gcaattgtcg actggctgcg
    11401 caaccagaac tatgccgtac ccttgctgcg tgccgtgccg gatcggatcg atgggcttgc
    11461 tgccggccgc cccgatgcca tcgcggcgat cgccacggat cgggcaagcc gggcgccacc
    11521 acccggtccg gatgttcctg ccctcggcta cgggctggcg tttggcgtca gctgtcgcga
    11581 aagctatccg ttcgcgacgc cggaggatct cgccgcggcc gggcgggaag cgtttccgca
    11641 ctatcccgcc tcgatacaag acgagggtgt cggcggctgg gcgtatttca acgaagactg
    11701 cggcgaggtc tggaaggtcc ccgccgctcc ggaggccatg catcagcccg tcgcgagcag
    11761 tatcccgacg ctgctcatct caggcagctt cgatacgttg acgtcgctcg ccggggcgaa
    11821 ggcggccgcg gcaaagctgt ccaacgcgac catcatcagc atcccggggg tcgggcattt
    11881 tgtctctccc gcgtcgccct gcgcccaggc ggtcatcgtc tcattcctga cgcccccgac
    11941 acgtcgtgcg tcggcgctct cacgccggag agcttcacct cgccggcgtc gccctaaaag
    12001 gcagggcatc ctcaatgttc ctgttttggt ctagaccagc cgcgaatcgg tctatcctgg
    12061 cccgatggtc ttcgagccca tcaggatcgg gaacgtcgcg atcgaccccg tgatcgcgtt
    12121 ttttgtgctt gcgggccttg cgggctttgc ccttgtcctg ctcgccgtcg cgctcttcgt
    12181 gcatatgagc cggcggcggc gcgagggcga agcgcaggcc gccgagcttg ccgaactgaa
    12241 ggggcgcttg cagagcttcg ccgagatcag cgtcgcgcgt cagggcgaca tcgcgcgcgc
    12301 cgtcaacgag cggctcgacc gcatgacgca cagggtcggc accgacctca acgagacctc
    12361 gcgcaagacg cacgagtcga tcgccaagct caacgagcgg ctggccgtca tcgacacggc
    12421 gcaaaggaac ctcaccgatc tctccaacaa catggtgagc ttgcaggaga tcctcgccaa
    12481 caagcaggcg cggggcgcct tcgggcagct ccgcatggag accatcgtca aggacggcct
    12541 gcccaagggc gcctacacgt tccaggccac gctctccaac ggcaagcggc ccgactgcct
    12601 gctgcacatg cccaacacca aggcgtccgt ggtcatcgac gccaagttcc cgctggaagg
    12661 cttcgaggcg ttccgcgccg cgcggcgcga ggaggagaag aaggaggcgg cgcggcgcgt
    12721 gcgcttcgat gtcgggcgtc acgtcgactc gatggccgag cgctacttca tccccggcga
    12781 gacgcaagat accgccatcc tgttcgttcc gtcggaagcg atctacgtcg acctctgcga
    12841 gcacttctct gatctcgtgc agagggcgca ccgcgcccgc atcgtgatct gcgcgcccaa
    12901 catgctcatg ctcgcggtgc agacaatgca ggcgatcctg aaggacgtgc agatgcggga
    12961 gcaagcgcat ctgatccagc gcgaagtggc gaggctcatg gacgacatgg gccgcttccg
    13021 cgagcgcgtg ctcgatctgc aacgtcattt cggccagacc aatcaggaca tcgagaagat
    13081 cctcacctcg tcggagaaga tcgccgagcg cgggcggaag atcgaaatgt tggagttcga
    13141 ggacggcgct cctgccgcga tcgaacaggt caacacatcc ccctcacccg gcgcggccgg
    13201 gccgcggccg gacgcgccac cctctcccgc gaggggagag ggacgcgagc gaaagcgaaa
    13261 cggcggcagc gcgcgccttc cgggcctcct ccccggcgaa tagcctctga acccgtcata
    13321 ccggcggaag ccggtatcca gattcgcgag cgcggtccat gccgccccct ggattccggc
    13381 ttccgccgga ataacctgtt atgagaaacc gatctctcgt catggccggg gttggcccgg
    13441 ccatccacga tttggccacc ctcagcaaga gattccacaa gcggtgtaga aattgaaacg
    13501 caacccccca cccggcgcgc cttgcgctca agcagcggcg cggcagcgca aacgaagcgt
    13561 cgcgccgacc tccccacgac ctgcctgcgc gaagccggag cctcgcttcg gcgaaggcag
    13621 ggggaggttt aaaagttgcc aggcgaggga tcgtcatggc cgggcttggc ccggccatcc
    13681 acgatttggc c
//
LOCUS       DASUQP010000061        23295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_573416,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000061 DASUQP010000000
VERSION     DASUQP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 23295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23295
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573416"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /gene="amrS"
                     /locus_tag="VFY74_02745"
     CDS             complement(<1..705)
                     /gene="amrS"
                     /locus_tag="VFY74_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX5930658.1"
                     /translation="MENTAMGASVPGRYWHRLDDGRLQCDLCPRECKLHEGQRGLCFV
                     RARAGDGIVLTTYGRSSGFCLDPIEKKPLNHFLPGTSVLSFGTAGCNLTCKFCQNWDI
                     SKARADDRLQSEASPEAIARAAVESGARSVAFTYNDPVIFLEYAVDVAAACREAGVKT
                     VAVTAGYIGAGARPEFFAAMDAANIDLKGFTENFYHRLCTGSLDAVLGTLRYVRHETK
                     TWLEITTLLIPGENDSS"
     gene            746..1564
                     /gene="amrB"
                     /locus_tag="VFY74_02750"
     CDS             746..1564
                     /gene="amrB"
                     /locus_tag="VFY74_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX5930659.1"
                     /translation="MFARAHGLTVRQPAVAGSFYPRGGKDLKTAVDALLSQADQARQS
                     GLVSVIAPHAGYMYSGPVAGSAFAPVASSGVFERVLLIGPAHYVPVRGVAAPSAASFA
                     TPLGTIALDRDAIATMVEAGLASIDDRAHAPEHALEVELPFLQAVLGRFTLIPLLAGD
                     AAPEEVAAIIDALLDERTLLVVSTDLSHYLDYASARKRDLATAETVERLAFDRLGPYD
                     ACGFAALNGALRAARGRGWSVKRLDLRNSGDTSGDRARVVGYGAWVFSAARPAA"
     gene            complement(1569..2090)
                     /locus_tag="VFY74_02755"
     CDS             complement(1569..2090)
                     /locus_tag="VFY74_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5930660.1"
                     /translation="MRSARLDATDWRILHNLQADGRITNVDLARKVGISAPACLRRAR
                     ALEEAGIIKGFTALLNDKALGFEVTAFAMIRLHSQAEADLIAFEERARSSAIVRDCYM
                     LSGEIDFLLRCVAPDLSAFQDFIIHELTAAPNVASVKTSLVIRASKHVEGVPVELAAE
                     ASAPAKPLARARR"
     gene            2193..3251
                     /locus_tag="VFY74_02760"
     CDS             2193..3251
                     /locus_tag="VFY74_02760"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HEX5930661.1"
                     /translation="MSANRSGNSVASQPAPSEPESVAQRATGSHQAAGEDTWIVTDGS
                     VGMEAQGIAVAEAVGLSFGLRRARVTGAMGFLPAPLQLLVPASRLLRFVASNAPLEAP
                     WPRLVISIGRRSVPIALAIKRLSHPHAFALHIQNPKVPARLFDLIAAPVHDNFEGPNV
                     IVTFGAVHSVTESRLAEEAARFAPHVKALPHPRIVVLLGGESQAFSFPPDAAAAFGAK
                     LATLARETGGGLLVTPSRRTRPDSLEALAGAVKDIPHFVWDGTGDNPYFAFLGLADAI
                     VVTEDSVNMVTEAAGTGKPVYVQALRGRSARLARFHRLMRERGATRPFEGRLESWRYT
                     PINDTERVAAAIRRSLGL"
     gene            3347..4309
                     /gene="trxB"
                     /locus_tag="VFY74_02765"
     CDS             3347..4309
                     /gene="trxB"
                     /locus_tag="VFY74_02765"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5930662.1"
                     /translation="MATRHAKVIIIGSGPAGYTAAIYAARAMLKPLLIEGIQPGGQMT
                     TTTDVENYPGFADVIQGPWLMEQMRAQAEHVGTEIIRDHVLEADVKRRPFWLKGDSGT
                     VYTADTLIIATGAKARWLGLPSEERFKGYGVSACATCDGFFFRGKKVIVVGGGNTAVE
                     EAIYLANIASYVTLVHRRGTLRAEKILQERLFANPKIEVMWNAELHEVLGTDNPLSVT
                     GAALKSTLTGEIVTLPVDGIFIAIGHEPQSELFKGQLDMKPSGYILVRPNSTATSVPG
                     VFAAGDVADDVYRQAVTAAGLGCMAALEAEKHLAGLERVAAAAE"
     gene            4511..5005
                     /locus_tag="VFY74_02770"
     CDS             4511..5005
                     /locus_tag="VFY74_02770"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5930663.1"
                     /translation="MTESTQAKVAADQARAAYRRTTEEFEQFARDGQVPEAVRVFAEK
                     NIAQTRELYDRSKDAFDKILESWEKTFDAAGQGAVALNRKVIDITQQNINSGFDFAKS
                     LVGAKTLGEAMALHSSYWQKQLGALRAQVDEMRELSTRVTADVAEPAKAEVKRGIGEL
                     QRTR"
     gene            5444..5587
                     /locus_tag="VFY74_02775"
     CDS             5444..5587
                     /locus_tag="VFY74_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930664.1"
                     /translation="MPVRYTSCTVWLCPVRLLTPTHAPATAGVEAKSTRSKAGKQDLI
                     ALL"
     gene            complement(5723..6508)
                     /locus_tag="VFY74_02780"
     CDS             complement(5723..6508)
                     /locus_tag="VFY74_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5930665.1"
                     /translation="MKRLLRLSVFAALALTSTAAFAAGDCAKITATGHPQYPVIAFKE
                     GDAIAGAAPMLVEAIAKQLNIPLQSKPMGSWADAQAAARDGKADMIFGIYHNDERAQY
                     LDYVQPAFMFDDVAVFVAKGKAFPFEGQDDLIGKKGVTNEGESYGNAFDAFMKDKLDV
                     ARTNGIDEAFNALLSGKADYLIAGYYPGLAEAAKAGVKDEVEVLDQALLTAEMFVAFS
                     KKSPCAAMADKFGQAITEMTTDGRFDTMLTDATAKWDAEQAKQ"
     gene            complement(6589..6888)
                     /locus_tag="VFY74_02785"
     CDS             complement(6589..6888)
                     /locus_tag="VFY74_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930666.1"
                     /translation="MTFYRALATSFLVFALPLLCLAPSAAFAQAGTITKGMQNNCAND
                     YKKFCGDYGLQTAALNLCMKKAGPSLSPACVQALVQAGKVSQADVDKIKAKMKGR"
     gene            7073..7456
                     /locus_tag="VFY74_02790"
     CDS             7073..7456
                     /locus_tag="VFY74_02790"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5930667.1"
                     /translation="MADKPSFEIPPELREIAEKNVEQARQAYGQFMDFVTKAMGAWAD
                     TAGHAEMAGFKAAQEKAIAFAKENAERSFALASDLARAKDVQEVLTLQSRYAQTQMQT
                     FGIQAQQLTWLMADAMRDFQPKSKG"
     gene            complement(7561..8790)
                     /locus_tag="VFY74_02795"
     CDS             complement(7561..8790)
                     /locus_tag="VFY74_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX5930668.1"
                     /translation="MTKIFPPLEIPATLAAGPGPGNTDPRVLERFAKTGVADHMQKDV
                     LRGMLEAKQMLRELWGTKNVYTYGVAGTGFSGLDCVLSLILPGDKVVTFPNGTFSGID
                     NLTIRMKAATAEELAADSLNPKPKSVVVVETPHGQSVTAETIERLAEHKPMWAFMAHW
                     ETGSGRINDLKAFSDACVKHGVMGIVDAVSSLGVADFNIDDYAGVVAWASCPQKGVLS
                     LPLTYAPVSFTDKAIEIVKKRGCRTYVHHPILDARHWGIVDGKDVETPVYHQTHSCYA
                     VAAFHEALRIILGYGRRRKASDYAYHERALRQTVEAMGCDVTSNMTSLVVLNLPGKLK
                     GKEKELVQGARAQGFGIWPTLSEPVQIRIGILNQLSPEQITEIVSRFADAMLAMGAEF
                     DRQKVLNQLKSYYAKAA"
     gene            9202..9972
                     /locus_tag="VFY74_02800"
     CDS             9202..9972
                     /locus_tag="VFY74_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5930669.1"
                     /translation="MVGLSSTPASAAPCGNNASGFESWKKAFAGEAAGNGLGPKAIST
                     LMGTKYSVGTIRADRGQKSFKLSLDQFMAKRGGPAIAARGRGMKSANAALFASIEKRF
                     GVPAGPLIAIWGMETAFGSFMGNQPTISAVATLAYDCRRPQYFTDQLYAALQLVDRGV
                     LSGATRGAMHGEVGQTQFLPKSVLLYAVDGDGNGVVNLNTKADALASTANFLRGHGWV
                     RGAGYQPGQPNFSAIQGWNAAGVYQQAIAIIGRRIDGS"
     gene            10025..10594
                     /locus_tag="VFY74_02805"
     CDS             10025..10594
                     /locus_tag="VFY74_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5930670.1"
                     /translation="MTGRKGQETEGPSRALAQALGKLIALVLVVVAVILIERYCTVEL
                     PPFGPDAKIVSVDGDTIRTANGAEYRLFGIDAPELAQTCTETNGKTWLCGRAAKAKLT
                     TLIKGGNVNCEARATDGFGRIVAICSAAGVPDLGEAMVRDGYAVDLGGAAGHPYRDAE
                     DEAETGKRGIWRGSFERPSDWRLSNPRSG"
     gene            10639..11238
                     /locus_tag="VFY74_02810"
     CDS             10639..11238
                     /locus_tag="VFY74_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX5930671.1"
                     /translation="MSEFELALSIALGVGLAAATGLRLFVPMLVASAAAYTGHLPLSE
                     NFAWLGTLPALILLGVAAVVEILAYYIPGVDNLLDALATPAAFVAGTIVAAAVMTDLP
                     PMVKWAAAIIAGGGVAGVTQSVTTLVRAKSTVFTGGLGNAPIATAELGGSLLVSLLAL
                     VAPLLTLLAIVLLLWLAIRWLRRIARSGQRSKEPRSGTA"
     gene            complement(11399..13447)
                     /locus_tag="VFY74_02815"
     CDS             complement(11399..13447)
                     /locus_tag="VFY74_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5930672.1"
                     /translation="MGQAQGQPARSARCIALVGPYLSGKTTLLESILARTGAITRQGS
                     TADGNTVGDASGEARHHGMSVELNAATVDFLGDSFTFLDCPGSIEFQADAVHALTACD
                     AAVVVCEPDEKKVPALQLILKQLEDRGIPHFLFINKIDKAEARVRDVVPMLQPASTKP
                     LVLRQIPIWENDIVTGFIDLALERAYVYREHAPSEVMAIPDALAASEKEARFSMLEKL
                     ADYDDELMEQLLEDVPPPRDKVFDDLSKEFREGMICPVFMGSAENGNGIFRLLKALRH
                     EAPFVQVTAKRLGVDKAPSCAFVSKTFHTAHGGKLTLARVLTGSFADGATVYGAAGEE
                     RISGIFTVMGQEPTKRGEAKAGDTVAFGRLDSVKTGTALTEEKGKQVAIETAATATPV
                     FGLAIAAKEKKDEVKLTAAIAKIIEEDPSISLTHSQDMGEMVLWGQGEMHLRVALERL
                     VRKYGIDASTRPRQIPYKETIRKTAEVRGRHKKQSGGHGQFGDVVVTIKPRQRGSGFE
                     FSDEITGGVVPKNYIPSVEIGVRDYLHAGPLGFPVVDVAVCLIDGSYHSVDSSDMAFR
                     QAGRIAMSEGMPQCSPVLLEPIMAVEIAVPSEATAKVNSIVSSRRGQILGFDSRAGWT
                     GWDVVEAHIPEAEIQNLIVELRSATAGVGTFHAKFDHLAELTGKAADHVLATRRSKAA
                     "
     gene            13658..14203
                     /locus_tag="VFY74_02820"
     CDS             13658..14203
                     /locus_tag="VFY74_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930673.1"
                     /translation="MRRFGAFVLPAGATLLLLALGSNNAAALVQALRPSSADERGLII
                     EAKIECGMFDGKFQCRAVSGGKQFGKSAGPGDDVEKEGTSPEAAPGGLMPGPDAGQAV
                     PVDPNACQGGMVGTPPNCQCPENSELLGGNCVRYTASACSNGLAVDALPQACRGVEEK
                     LSCKLREDGLKDCCCVTYNRF"
     gene            complement(14223..14630)
                     /locus_tag="VFY74_02825"
     CDS             complement(14223..14630)
                     /locus_tag="VFY74_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5930674.1"
                     /translation="MKPAFTHCALHVRDLDRSIAFYRSYCGLELVNEHGKGNSRTVWL
                     ASPGAERNFVLVLLGGGPAHAQEDGDMTHYGFGVPAREDVDRIAERARADGCLHWEPR
                     EYAPPTGYLCAVKDPSGHIIEFSHGQPLGPHQD"
     gene            complement(14639..15742)
                     /gene="mtnA"
                     /locus_tag="VFY74_02830"
     CDS             complement(14639..15742)
                     /gene="mtnA"
                     /locus_tag="VFY74_02830"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5930675.1"
                     /translation="MKIGGKSYRTIWLNADGKSVEIIDQTKLPHVFETVTLRTLDDAA
                     RAIATMQVRGAPLIGATAAYGVALALRDDASDQALDRACATLAATRPTAINLRWALDE
                     MQKAVRNQPRDKRVAAAFARAAAICEEDVETNRKIGEHGLGIIKAHAEKKGGATVNIL
                     THCNAGWLACVDWGTALAPIYRAHDEGIGVHVWVDETRPRNQGAALTAFELGAHGVPH
                     TLIADNAGGHLMQKRRVDLCIVGTDRTTATGDVANKIGTYLKALAARDNQVPFFVALP
                     YATIDWTLADGSTIPIEERAAEEVTRIAGRLPDGRVATVEIAPPETQAANYGFDVTPA
                     RLVTGFITERGVAPATRDALAALYPEHGKGQGA"
     gene            complement(15882..16421)
                     /locus_tag="VFY74_02835"
     CDS             complement(15882..16421)
                     /locus_tag="VFY74_02835"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX5930676.1"
                     /translation="MSADFNLKDLVRTIPDYPKPGIMFRDITTLLGHANGFKACIEQL
                     VRPFTDRRVQSVAGIEARGFILGGAVADRLHCGFVPIRKKGKLPAKAIGQDYELEYGI
                     DVIEVHEDAIKAGEPVLLVDDLIATGGTAEAAVRLLRKLKADVVGATFVIDLPDLGGS
                     TRLARLGVECQSLMSFAGA"
     gene            complement(16678..17553)
                     /locus_tag="VFY74_02840"
     CDS             complement(16678..17553)
                     /locus_tag="VFY74_02840"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX5930677.1"
                     /translation="MVKAVLGVIGGSGLYDLPGLKNVREEPVESPWGEPSAPLRIGEI
                     DGLPIVFLSRHDKGHRLSPSDINYRANIDVLKRAGVTDLVSLSACGSFKEELPPGTFV
                     LVDQFVDRTYHRHNSFFGRGCVAHISMAHPVSPRLRLHLAEAAEQEGIAVVRSGTYVC
                     IEGPQFSTLAESLTYKTLGYSVIGMTNLPEAKLAREAEICYATVAMVTDYDCWHPDHD
                     AVTVTDIIKVLMANAEKGAQLIARLARSFPREHEPCPIGSDRALDTAIITAPEARDPA
                     LLRKLDAVAGRVLKG"
     gene            complement(17596..18420)
                     /locus_tag="VFY74_02845"
     CDS             complement(17596..18420)
                     /locus_tag="VFY74_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX5930678.1"
                     /translation="MRSSFSRLAAAGALLLACAGPSASEDASGTSSNNHEPEIARQSW
                     SFDPPFGTYDNAQLQRGFKVYQTVCANCHSMRLLSYRTLGERGGPEFSPEAVEILASQ
                     VQVTDGPNDKGEMFQRPARPSDRFRSPFANEQLARATNNGALPPDLSVIAKARPGGHD
                     YIYALLTGYSPAPQGFELAQGMHYNAAFPGHQIAMTPPLRDGTVTYTDGTKPTVDNYA
                     RDVSAFLMWAAEPKLEERHAVGTRVMIFLLAFVVIMYLAKRSVWARLHHGEKERAA"
     gene            complement(18420..19712)
                     /locus_tag="VFY74_02850"
     CDS             complement(18420..19712)
                     /locus_tag="VFY74_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX5930679.1"
                     /translation="MAHPSSYQPKSGIERWLDERLPVVRVVHDQFMVFPTPRNLNYWW
                     TFGGILTFMLAVQIATGVVLAMHYVPTEAGAFASVEKIMRDVNWGWLLRYSHAVGASM
                     FFLAVYIHTFRGLYYGSYKAPREVLWILGVLILMLMIATAFMGYSLPWGQMSFWAVTV
                     ITNLFSSLDSVIPGLGTTLVEWIWGGFAVGEPTLNRLYALHYLFSFLIAGVVVLHIWA
                     LHIPGNNNPTGIDVKSTRDTVPFHPYYTTKDGFALVAFILLFAALVFYAPNFLGHPDN
                     YIPANPLQTPTHIVPEWYFLPFYAILRAIPNKLLGVAALNGSILILFLVPWLDTSKVR
                     STRYRPIYKWFFWAFVVTCVALGYLGAQAPEGLYLVLGRILTAYYFAFFLVVMPVVGR
                     IEKPRALPGSITEPVLGPKAERASPARTPHKRKPETVG"
     gene            complement(19721..20320)
                     /gene="petA"
                     /locus_tag="VFY74_02855"
     CDS             complement(19721..20320)
                     /gene="petA"
                     /locus_tag="VFY74_02855"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX5930680.1"
                     /translation="MASHDIPVHASPDDDEPTRRDVLLVVAGGFAAVGGAIALWPLLD
                     QMNPDASTLSLATTEVDISPIEPGQALTVMWRGKPIFIRHRTEQEIVKAKDTPLDDLI
                     DPLARNANLPDHAPATDDNRAAKDREPWLVMVGVCTHLGCIPKGQAVGDYKGEYGGWF
                     CPCHGSQYDTAGRVRIGPAPENLAIPPYSFVSEQKLKIG"
     gene            20676..21596
                     /gene="hemF"
                     /locus_tag="VFY74_02860"
     CDS             20676..21596
                     /gene="hemF"
                     /locus_tag="VFY74_02860"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX5930681.1"
                     /translation="MDQKASKQPTSPEPFSPARKERARLWFEHLRDEICAALEAVEDA
                     AEGLPGCEGKAPGRFVKTPWTRTEKDGSDGGGGAMALMSGRVFEKVGCHTSTVHGTLA
                     PEFAKQIPGAEADPRFWASGISFIGHPVNPNAPAAHMNTRMVVTSKGWFGGGGDLTPV
                     LMRRRTQDDEDSRDFHAAMKAACDGEASADYDRFKKWCDEYFYLPHRGEMRGIGGIFY
                     DYLDTGDWKADFDFTQKVGRAFLGIYPEIVRRNIGTPWTEADRDEQLVRRGRYVEYNL
                     LYDRGTLFGLKTGGNVEAILSSLPPLVRWP"
     gene            21683..21832
                     /locus_tag="VFY74_02865"
     CDS             21683..21832
                     /locus_tag="VFY74_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930682.1"
                     /translation="MLRALIEQGLRRLLAEKARGRTFRLRKASVNGRGLHPDMQGATW
                     EQVSR"
     gene            complement(21926..22090)
                     /locus_tag="VFY74_02870"
     CDS             complement(21926..22090)
                     /locus_tag="VFY74_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930683.1"
                     /translation="MGKIVVILLLTALAILMARLVYDAPVIQTRVGELIGTTAPAPVA
                     PPLMYAGIRG"
     gene            22440..22658
                     /locus_tag="VFY74_02875"
     CDS             22440..22658
                     /locus_tag="VFY74_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5930684.1"
                     /translation="MARVNGTVKFFNHARGFGFIQPEDGGKDVFVHASALERSGVPAL
                     NEGDKVSFEIEDDRRGRGKQAANVQLAN"
     gene            22947..23282
                     /locus_tag="VFY74_02880"
     CDS             22947..23282
                     /locus_tag="VFY74_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEX5930685.1"
                     /translation="MDDGTLKAIAASALIGAALLGAPAPAQAGNGDAWGAGLFGFGVG
                     AIIGSALAPREVYVVPPPPPAYYYGPVAYGPPPWTPDWYAYCSSRYQSFDPSTGYFMG
                     YDGYPHLCR"
BASE COUNT         4081 a         7390 c         7606 g         4218 t
ORIGIN      
        1 tgacgaatcg ttttcccccg ggatgagaag cgtggtgatc tcgagccagg tcttggtctc
       61 gtgcctgacg tagcggaggg tgccgagcac ggcgtcgagc gagccggtgc agagccggtg
      121 atagaagttc tcggtgaagc ctttgaggtc gatattggcg gcatccatcg ccgcgaagaa
      181 ctccggccgc gcgccggcgc cgatataccc tgccgtgacg gcgaccgtct tcacgccggc
      241 ctcccggcat gcggcggcaa cgtcgacggc atattcgagg aagatgacag ggtcgttgta
      301 ggtgaaggcg accgaccgcg cgccgctttc gaccgctgcc cgcgcgatag cctcgggtga
      361 agcctcgctc tgcaaacgat cgtcggcgcg ggctttggag atgtcccagt tctggcagaa
      421 cttgcaggtc agattgcagc ccgcggtgcc gaaagagagg accgaggttc ccggcaggaa
      481 gtggttgagg ggcttcttct cgatgggatc gaggcagaag cctgacgacc ggccataggt
      541 cgtgagcacg atcccatcgc ccgcgcgcgc gcgtacgaag caaaggcctc tctggccctc
      601 atgcagcttg cattcgcgcg ggcagaggtc gcattgcagg cggccgtcat cgagcctgtg
      661 ccagtagcgg cccggcaccg aggcgcccat cgcggtgttt tccatggtag aaacttgggc
      721 aggttctcat cggagtcaac ggccgatgtt cgctcgcgcg cacgggctga cggtgcggca
      781 accggcggtc gccggcagct tctatccgcg cgggggcaag gacctgaaaa ccgcggtcga
      841 tgcgctcctt tcgcaggccg accaagcgcg ccaatcgggc ctcgtcagcg tcatcgcgcc
      901 gcatgcgggc tacatgtatt ccggtccggt cgcgggaagc gcctttgcgc cggttgcatc
      961 gtcgggcgtc ttcgagcggg tgctgctgat cggccccgcc cactacgttc cggtgcgagg
     1021 cgttgccgcg ccctccgccg cttcgttcgc cacgccgttg ggcacgatcg cgctcgaccg
     1081 agacgccatc gccacgatgg tggaagcagg actcgccagc atcgacgatc gggcccacgc
     1141 gccggaacat gcgcttgagg tcgagctgcc cttcctgcaa gcggtgctcg ggcgcttcac
     1201 gctcattccg ctgctcgcgg gcgatgccgc ccctgaagag gttgcggcaa tcatcgatgc
     1261 gctgctggat gagcgcacgc tcctcgtggt cagcaccgac ttgtcgcact atctcgacta
     1321 tgcctcggcg cggaaacgcg atcttgccac ggccgagacg gtcgagcggc tcgcgttcga
     1381 tcggctaggc ccatacgacg cgtgcggttt cgctgcgctg aacggcgcgc tccgcgccgc
     1441 acgcggccgg gggtggagcg tcaagcgcct cgacttgcgc aattccggcg acacgagcgg
     1501 cgatcgcgcc cgcgttgtcg gctatggcgc gtgggtcttt tccgccgcaa ggccggccgc
     1561 ttagcgcgtc acctgcgcgc ccgtgccaag ggcttcgcag gggcgcttgc ctcggcggca
     1621 agctcgaccg gcacgccctc cacatgtttg gaggcgcgaa tgacgagcga tgtcttcacg
     1681 ctcgccacgt tgggggcggc ggtcagctcg tggatgatga agtcctggaa cgcactcaga
     1741 tcgggcgcga cgcagcgcag caggaaatcg atctcgccgg aaagcatgta gcaatcccgc
     1801 acgatggccg aactccgcgc gcgctcctcg aatgcgatca ggtcagcctc ggcttgactg
     1861 tgcaaccgga tcatggcgaa agccgtaacc tcgaagccga gcgccttgtc gttcaggaga
     1921 gcggtgaagc ctttgatgat gcccgcctcc tcgagcgcgc gtgcgcggcg aaggcaggcc
     1981 ggcgccgaga tcccgacctt gcgggcgaga tcgacattgg tgatgcggcc gtcggcctgc
     2041 aaattgtgca agattcgcca gtcggtggca tcgagccttg ctgaacgcat gcgtctctcc
     2101 ctggacgatg gcggggatcg cgggctagaa ggcagcctgc ttcggctttc agcgcaattt
     2161 taattcctcc agccaaaaga atcgaaaaga aattgagcgc aaatcgttcc ggaaattctg
     2221 tcgcctcgca accggcaccc tccgagccgg agtcggttgc gcagcgggcc accgggagcc
     2281 atcaggccgc aggggaagac acctggatcg tcaccgacgg ctcggtcggc atggaggcgc
     2341 aaggcattgc ggtggcggaa gcggtcggtc tctccttcgg cttgcggcgt gcgcgagtaa
     2401 ccggagcgat ggggtttctg ccggcgccct tgcagcttct cgtgccggcg tcccgcctcc
     2461 tccgtttcgt cgccagcaat gcgccgctcg aagcgccttg gccgcgcctc gtcatctcca
     2521 tcggcaggcg cagcgtgccg atcgcccttg cgatcaagcg gctctctcac cctcacgcct
     2581 tcgcgctcca tatccagaac ccgaaggtcc cggcccggct cttcgatctc atcgccgcac
     2641 ccgtccacga caatttcgag ggtccgaacg tgatcgtcac cttcggcgcg gtgcatagcg
     2701 tcaccgaaag ccgcctcgcg gaggaagcgg cgcgtttcgc cccgcacgtg aaagccctcc
     2761 cccatcctcg tatcgtcgtc ctgctcggtg gcgagagcca agccttcagc tttccgcccg
     2821 acgccgctgc cgctttcggc gccaagctcg cgacgctcgc gagggaaacg gggggcggcc
     2881 tcctcgtgac cccgtcgcgc cgcacacggc ccgactcgct cgaggcgctc gccggggccg
     2941 tcaaggatat cccccacttc gtctgggacg gcaccggcga caatccctac ttcgccttcc
     3001 tcgggcttgc ggacgccatc gtcgtcaccg aggattcggt caacatggtt accgaggcgg
     3061 ccggcaccgg caaaccggtc tatgtccagg cgcttcgggg ccgttcggcc cggctcgccc
     3121 gcttccatcg cctgatgcgg gaacgcggcg caacgcgccc cttcgagggg cgcctcgaat
     3181 catggcgcta cactcccatc aacgacactg agcgcgtggc cgccgccatc cgccgcagct
     3241 tgggcctctg aatccaaagg acaatcgatg ctcaccgggc atcctgggtt gcagcccggg
     3301 cctcttgtgc ttacgtgtcg tctccttccc gccttgagag gcaatcatgg ccacgcgtca
     3361 cgcaaaggtg atcatcatcg gctcaggccc ggccggctat accgccgcca tctatgccgc
     3421 ccgcgccatg ctcaagccgc tcctgatcga gggcattcag cccggcgggc agatgaccac
     3481 aaccaccgat gttgagaact atcccggctt cgccgacgtg atccaaggcc cttggctcat
     3541 ggagcagatg cgcgcgcagg ccgagcatgt cggaaccgaa atcattcgcg accatgttct
     3601 cgaagcggat gtgaagcggc gcccgttctg gctcaagggc gatagcggca ccgtgtatac
     3661 cgccgacacc ctcatcatcg cgaccggcgc gaaagcgcgc tggcttgggc tgccttccga
     3721 ggagcgtttc aagggttatg gcgtctcggc ctgcgccacc tgcgacggct tcttcttccg
     3781 cggcaagaaa gtgatcgtgg tcggcggcgg caataccgcc gtcgaggagg cgatttatct
     3841 agccaatatc gcgagttacg tgaccctagt gcaccggaga ggcacgcttc gcgccgagaa
     3901 gatcctgcag gagcggctgt tcgccaatcc caagatcgag gtgatgtgga acgccgagct
     3961 tcatgaagtt ctcggtaccg acaatccgct ctcggtcacc ggcgccgcgc tcaagagcac
     4021 gctgaccggc gagatcgtca cactgcccgt cgacggcatc ttcatcgcca tcgggcacga
     4081 accgcagagc gaactgttca agggccagct cgacatgaag ccctccggct atatcctggt
     4141 gaggccgaac tcgacggcaa cgagcgtgcc gggcgtcttt gcggcgggcg acgtcgccga
     4201 cgacgtctac cgccaggcgg tgaccgcagc gggactcggt tgcatggctg cgctcgaggc
     4261 cgagaaacat ctcgccggcc tcgagcgggt tgcggcggcc gccgaatagg cgctccgcgt
     4321 gccccctatt attttgcact gattggcgat gaacgcgcgg cgctaagtcc cggatgttca
     4381 attgaattgg cgcggcacga cgcgcaaaaa aaattcccgg cgaagcgcga tgactttcag
     4441 ccggtaaatc gttaatctta aggagtcagc cgacgctgat ttccccctat tcagcataag
     4501 aggccgtacc atgaccgagt ccactcaagc gaaagtggcc gccgaccagg cgcgtgccgc
     4561 gtatcgcaga acgactgagg agttcgaaca gttcgccaga gatggtcaag tgcccgaggc
     4621 cgttcgggtc tttgccgaga agaatatcgc tcaaacccga gagctttacg accgttcgaa
     4681 agatgctttc gacaaaattc tggagagctg ggaaaagacc tttgatgccg ccggccaggg
     4741 cgccgttgcg ctcaatcgga aagtcatcga tatcacgcag cagaacatca attcgggctt
     4801 cgatttcgcc aagagccttg tcggcgccaa gaccctgggc gaagcgatgg ccctgcattc
     4861 gtcctactgg cagaaacaac tcggcgcctt aagggcgcaa gtggacgaga tgcgcgagct
     4921 ttccacgcgc gtcacggccg acgtcgccga gcctgccaaa gctgaggtca agcgcggcat
     4981 cggcgaactc cagaggacac gctagcgtcc tctgctgtct gatctagtct ccgcggggag
     5041 agggttggct gtttgcgccg accctttccg gtcgcgcgtc accgcgggat tcaacgactt
     5101 aagctttagc gctgggccga gctaacgcac cggctcgcag tcttgctcca aaacagcttg
     5161 ccttgaccgg catagtcgac ggcggtctcg ctgttgacat ccctggaaac gacataggtt
     5221 ccgattgccg ccacaccgct cgccgacaat cccatccgcc tgagcgcaca gcaattggcc
     5281 gcgatctcgt ctttatgccc attgtgatgg cagcattcgt gattgcggat gaagcttttg
     5341 aaatgggggt ggggcgttgg cgtatcggcc gtaatagatg atctggggac gtccgctcgc
     5401 gcgatcgtgc gtcgcctcgg caaagaatcg ccgagggtgg ctgatgcccg tccggtacac
     5461 ctcctgcacc gtctggctct gcccggtccg gctgttgacg ccgacgcatg cgccggcgac
     5521 cgcgggcgta gaggccaaga gcacgagaag caaggcgggg aaacaggacc tcatcgccct
     5581 gttgtagcgc tggctttttc ggcagatgtt aacgcgggtg gcggatggcg ggcgccggcc
     5641 gttcggagat tttacgcgcc tccagacgcg cgcggcgcgc gtacgttaat cagtttcgca
     5701 gcggccgtcc tctgaggcgc ggttattgct tcgcctgttc ggcgtcccat ttcgcggtcg
     5761 catcggtcag catcgtgtcg aagcgaccgt cggtcgtcat ctccgtgatg gcctggccaa
     5821 acttgtcagc cattgcggcg cagggcgact tcttcgagaa ggcgacgaac atttcggcgg
     5881 tgaggagcgc ctggtcaaga acctcgacct catccttgac cccggctttg gcggcctcgg
     5941 cgagacccgg ataatagccc gcgatgagat agtcggcctt gcccgacaac agcgcattga
     6001 acgcctcgtc gataccgttc gtgcgggcga cgtcgagctt gtctttcatg aaggcgtcga
     6061 acgcgttgcc gtagctttcg ccctcattgg tcacgccctt cttgccgatc aaatcgtcct
     6121 gaccctcgaa cgggaacgcc ttgcccttgg caacgaagac cgccacgtcg tcaaacatga
     6181 aggccggctg gacataatcc aaatattggg cccgttcgtc gttgtggtag atgccgaaga
     6241 tcatgtcggc tttgccgtcg cgggcggccg cttgcgcgtc tgcccaagac cccatgggtt
     6301 tggattggag cggaatgttg agctgcttgg cgatggcctc aacgagcatg ggcgccgcgc
     6361 ccgcgatcgc gtcgccttcc ttgaaggcga tgaccggata ttgcgggtga ccggttgctg
     6421 tgatcttggc gcaatcgccg gcggcgaatg cggccgtgct cgtgagcgcc agggccgcga
     6481 acacgctcaa gcggagaagt ctcttcatgg tgaaagttcc ttgttctctt gatcggggtg
     6541 ggggcgcgtg tcgtcgggcg ggaggcagcc gctaagcagg atcttgctct accgcccttt
     6601 catcttcgcc ttgatcttat ccacgtccgc ttgcgaaact tttccggcct ggaccagcgc
     6661 ttgcacgcag gcgggcgaga gcgacgggcc cgctttcttc atgcacagat tgagcgcggc
     6721 tgtttgcagt ccgtagtcgc cgcagaactt cttgtagtcg ttggcgcaat tattctgcat
     6781 gcccttggtg atggtcccag cctgcgcgaa cgccgcagac ggggccaagc acagcaaagg
     6841 caaggcgaag acaaggaagc tggtcgccag agcgcgatag aatgtcatcc gggatatccc
     6901 tctatgaccc agccagggtt ctagcacgct tggcgcgcct gggcacttaa agtctcttcg
     6961 ggctccgggg gttaaccaaa cgcgcccctt tcccctcgga atgcagtagt ctagacggtg
     7021 tagtggagcg ggctcagctc gccttgtaaa cggcagtctc tgagaggacc ccatggcaga
     7081 caagccttcg ttcgaaatac cgcctgagct gcgcgaaatc gccgagaaga atgtcgagca
     7141 ggcgcgtcag gcctacggtc agttcatgga tttcgtgacg aaagccatgg gggcctgggc
     7201 cgatacggcg ggccatgccg agatggcggg atttaaggcc gcgcaggaaa aagccatcgc
     7261 tttcgccaag gagaacgccg agcgatcctt cgccttggcg agcgacctcg cccgcgccaa
     7321 ggatgtgcag gaggtgctga ccctccagag ccgctacgcg cagacccaga tgcagacctt
     7381 cgggatccag gcgcagcagc tcacttggct catggctgac gccatgcgcg acttccagcc
     7441 caagagcaag ggttgaacga agcgcccgcg gacacggaac gcgcccgcgt cggttcggaa
     7501 aaggcccggc gaaccgccgg gccttttgcc ttcgagagga ctcagaggtg ctgcgaagcg
     7561 ctaggccgcc ttggcgtagt acgacttgag ctggttcaac accttctgcc tgtcgaactc
     7621 cgcgcccatg gcgagcatgg catcggcgaa gcggctcacg atctcggtaa tctgttcggg
     7681 gctcagctga ttgagaatgc cgatgcggat ctggaccggc tcggacaagg tcggccagat
     7741 gccgaagcct tgcgcccgcg cgccttgcac cagctccttc tctttgccct tcagtttgcc
     7801 gggcaggttg agcacgacga ggctcgtcat gttcgaggtg acgtcgcagc ccatggcttc
     7861 gacggtctgg cgcagcgccc tctcgtgata ggcgtagtcg gaagctttgc ggcggcggcc
     7921 ataaccgaga atgatgcgca gcgcctcgtg gaaggcggcg accgcgtagc acgaatgtgt
     7981 ctggtgatag acgggtgtct cgacgtcctt gccgtcgacg atgccccaat ggcgcgcgtc
     8041 gaggatcggg tgatgcacat aggtgcggca cccgcgcttc ttgacgatct cgatcgcctt
     8101 gtcggtgaag ctgaccgggg cataagtgag cggcagcgac agcacgccct tttgcggaca
     8161 cgaggcccag gccaccacgc ccgcatagtc gtcgatgttg aaatcggcga cgccgaggct
     8221 cgataccgcg tcgacaatgc ccatgacgcc atgcttgacg caggcgtcgc tgaaggcctt
     8281 gaggtcgttg atgcggcccg agcccgtctc ccaatgggcc atgaaggccc acatcggctt
     8341 atgctcggcg agcctctcga tggtctcggc ggtgacggac tggccgtgcg gagtttcgac
     8401 caccaccacg ctcttcggct tcgggttgag cgaatccgcc gcgagctcct cggcggttgc
     8461 ggccttcatg cggatggtaa gattgtcgat gccggagaac gtgccgttgg ggaacgtcac
     8521 gaccttgtcg cccggcaaaa tcaaagagag cacgcaatcg aggcccgaga agcccgtgcc
     8581 ggcaacgccg taggtataga cgttcttggt cccccagagt tcgcgcagca tctgcttggc
     8641 ttcgagcatg ccacgcagca cgtccttctg catgtgatcg gcgacgccgg tcttggcaaa
     8701 acgctcgaga acccttgggt ccgtgttccc cggccctggg ccggcggcga gcgttgcggg
     8761 gatttcaagc ggcgggaaga tcttcgtcat gggaacaccc tctggtctaa cgtcgcacaa
     8821 ggaggcggca caatcgtcta tccgtcaaga cgcgctgcgg caagaccggc aaagtctgga
     8881 tttctgcgcc cgcctgcttt cccaagtgca cgtgagcgcc ggaaagagag agatcgaacc
     8941 ccaggaaagg aagaagcccc gtacgggggg agcggggctt cttccaaata gttccctgcg
     9001 gggacgatga tgggagggaa cgccatcacc gcgcctcgaa cttagcgcat ttccgacaag
     9061 agtcgggact tttttgcaaa aatggttaag gttaacaatg ccacaggggc accggcctgc
     9121 ctggaaaggc ctaaaacagc gggacgcggc aggatctcca tgacgatcaa gtctctgagt
     9181 ttggccgtcc ttttggcggc catggtcggg ctgtcgagca ctccggcgtc cgccgcgcct
     9241 tgcggcaaca acgcttccgg cttcgagagc tggaagaagg ccttcgccgg cgaagccgcc
     9301 ggcaacgggc tcgggcccaa ggcgatctcc acgctgatgg ggacgaaata ttcggtgggg
     9361 acgatcaggg ccgaccgcgg gcagaagagc ttcaagctga gcctcgatca gttcatggcg
     9421 aagagaggcg gtccggccat tgccgcccgg ggtagaggga tgaaatcggc gaacgccgcc
     9481 ctgttcgcct cgatcgagaa gcggtttggc gttccggccg gcccccttat cgccatttgg
     9541 gggatggaga ccgccttcgg cagcttcatg gggaaccagc cgacgatctc cgcggtcgcg
     9601 accctcgctt acgactgccg ccggccccaa tattttaccg accagctcta cgcggcgctt
     9661 cagctcgtcg accgcggcgt gctcagcggc gcgacgcgcg gcgccatgca tggcgaggtc
     9721 ggccagaccc agttcctgcc gaagagcgtg cttctctatg ccgtcgacgg cgacggcaac
     9781 ggcgtcgtca atctcaacac caaggccgac gcgcttgcct caaccgccaa tttcctcaga
     9841 ggccatggct gggtcagagg cgccggctat caacccggtc agccgaactt cagcgccatc
     9901 cagggctgga acgcggccgg cgtctaccag caggccatcg ccatcatcgg caggcgcatc
     9961 gatgggagct agccgcaagg cgcaatgcct cgacgcgcgg gcgaatctgc tgcttccctg
    10021 acgcatgacg ggacggaaag gccaggagac cgagggacca tcccgcgcgc ttgcccaagc
    10081 gctcggcaag ctgatcgcgc tcgtgctggt ggtcgtcgcg gtgatcctga tcgaacgcta
    10141 ttgcaccgtc gagctgcccc ccttcggccc cgatgcgaag atcgtctccg tcgacggcga
    10201 caccatccgc acggccaatg gtgccgaata ccgcctcttt ggtatcgatg cgccggagct
    10261 tgcgcagact tgcaccgaga cgaacggcaa gacctggcta tgcggccgcg ccgcgaaagc
    10321 caagctcacc acgctcatca agggcggcaa tgtgaattgc gaggcccgcg ccacagacgg
    10381 cttcggccgc atcgtcgcga tctgcagcgc ggcaggcgtg ccggatctcg gcgaggccat
    10441 ggtgcgcgac ggctatgccg tcgatctcgg cggagcggcc ggtcatccct atcgcgacgc
    10501 cgaggatgag gctgagaccg gcaagcgcgg catctggcgg ggatccttcg agcgcccctc
    10561 cgactggcgt ctgtcaaatc cgcgctccgg ctgatgccgt tgtctggcgc taaactgcca
    10621 gcgtggtaat tttcggccat gtctgaattc gaactcgccc tgtcgatcgc gcttggcgtc
    10681 gggcttgcgg cagcaaccgg gcttcggttg ttcgtgccga tgctcgtggc aagtgctgcc
    10741 gcctataccg ggcatctccc attaagcgaa aactttgcct ggctcggcac attgccggcg
    10801 ctgatcctgc tcggcgtggc ggcggtggtg gagatcctcg cctattacat tcccggcgtc
    10861 gacaatctgc ttgacgctct ggccacgccc gccgccttcg tcgccggcac gatcgttgcc
    10921 gcggcggtca tgaccgacct gccgcccatg gtgaaatggg ctgcggcgat cattgccggc
    10981 ggcggcgttg ccggcgtgac ccaatccgtg accacgctcg tccgggcgaa atcgaccgtg
    11041 ttcaccggcg ggcttggcaa cgcgcccatc gccacggccg agctcggcgg ttcgctcctc
    11101 gtctcgctgc tcgcgctcgt agcaccgctt ctgacgcttc tcgccatcgt tcttctgttg
    11161 tggctggcga tccgttggct gcgccggatt gcgcgaagcg ggcagcggtc gaaagagccg
    11221 agatccggta cggcctgaac ttgccgtggc gccggtgttt gatcggcgag gctaggccat
    11281 gaccttcgga ggcagttccg gctcaaggtc cgcttcaccc ggcggcgcaa acacgctgat
    11341 gcgattcagg ccctccgcct ccaaagcctt agtcaatcgt tacaggccga attcaccctt
    11401 aagccgcctt ggagcgccgt gtcgcgagca cgtgatcggc agcctttccg gttaactctg
    11461 ccagatgatc gaatttggca tggaaggtgc cgacccccgc cgtcgccgag cggagctcga
    11521 cgatcaagtt ctggatctcg gcctcgggga tatgcgcctc gaccacgtcc cagccggtcc
    11581 agcccgcgcg cgaatcgaag ccgaggatct gcccgcgtcg cgacgacacg atggagttga
    11641 ccttcgccgt ggcctccgac ggcaccgcga tctcgaccgc catgatcggc tcgagcagca
    11701 cgggcgagca ttgcggcatg ccctcgctca tggcgatgcg gccggcctgg cggaacgcca
    11761 tgtccgagga atcgacggag tggtaggacc cgtcgatcag gcacacggcg acatcgacca
    11821 cgggaaagcc gagcgggccc gcgtggagat agtcgcgcac gccgatttcg accgatggaa
    11881 tatagttctt cggcacgacg ccgccggtaa tctcgtcgga aaactcgaag ccgctcccgc
    11941 gctggcgcgg cttgatcgtg accacgacat cgccgaattg gccgtggccg ccggactgtt
    12001 tcttgtggcg gccgcgcact tcggccgttt tgcggatggt ctccttgtag gggatttgac
    12061 ggggccgggt cgaggcatcg atcccgtatt tgcgtacgag ccgctcgagc gccacgcgca
    12121 gatgcatctc gccctgcccc cacaacacca tctcgcccat gtcctggctg tgggtgagcg
    12181 agatcgaggg gtcctcctcg atgatcttgg cgatggccgc agtgagcttc acctcgtcct
    12241 ttttctcctt tgcggcgatg gcgaggccga acacgggggt agcggtggcg gcggtttcga
    12301 tcgcgacctg cttgcccttc tcttcggtca gcgcggttcc ggtcttgacg ctgtcgagcc
    12361 ggccgaaggc gacggtgtcg ccggcctttg cctcgccgcg cttggtgggc tcctggccca
    12421 tgacggtgaa aatgcccgag atgcgctctt cgccggccgc gccatagacc gtcgcgccat
    12481 cggcgaaact gccggtgagc acgcgggcga gcgtcagctt gccgccatgg gcggtgtgga
    12541 aggtcttgga cacgaaggcg caagagggcg ccttgtcgac gccaaggcgc ttagctgtga
    12601 cttggacaaa aggggcttcg tgcctcagcg ccttgagcag gcggaagatg ccgttgccgt
    12661 tctccgccga acccatgaac acggggcaga tcatgccttc gcggaactcc ttggagagat
    12721 cgtcgaacac cttgtcgcgc ggcggcggca catcctccag gagttgctcc atcagctcgt
    12781 cgtcgtagtc ggcaagcttc tcgagcatgg agaagcgcgc ctccttctcg ctggcggcga
    12841 gagcgtcggg gatcgccatg acctcgctcg gcgcgtgctc gcggtagaca taggcgcgct
    12901 ccagagcgag atcgataaag ccggtgacga tgtcgttctc ccagatggga atttgccgga
    12961 gaacgagcgg cttggtgctt gcgggctgga gcatcggcac gacatcgcgc acgcgggctt
    13021 cggccttgtc gatcttattg atgaacagga aatgcgggat gccgcgatcc tcgagctgtt
    13081 tgaggatcag ctggagcgcg ggaaccttct tctcgtcggg ctcgcacacg acgacggcgg
    13141 catcgcaagc ggtgagcgca tggaccgcgt cggcctgaaa ctcgatcgaa ccgggacagt
    13201 cgaggaaggt gaagctatcg ccaagaaaat cgacggtggc cgcgttgagc tctacgctca
    13261 tgccgtgatg ccgggcctct ccggaagcgt cgccgaccgt gttgccgtcc gcagtcgaac
    13321 cctgtcgggt aatggcgccg gtacgagcga gaatggattc gagaagcgtc gtcttgccgc
    13381 tgagataggg gccaaccagt gcgatgcagc gggcgctgcg ggctggttgg ccctgcgctt
    13441 ggcccatgat ctcctccctt gcggttatcc cgcgcggaga aggctcctga cgcacccacc
    13501 cgcgcaagcc ttaagggtgc catggtgaca agcggagcgg cctctggcaa ggcccccgtt
    13561 cgaccgccct ggtcctccga agtgcggctg gcatgggctc gcgcctgtgt cattatgtgg
    13621 gcaacgcgat gcgggcgacg cagcggaggg agtgttcatg cgcaggtttg gcgccttcgt
    13681 gctgccggca ggagcaactc ttctgctctt ggctctcggc agcaacaacg cggcggctct
    13741 cgtccaggcg ctgaggccat catccgctga cgagcgaggg ctcatcattg aggccaagat
    13801 cgaatgcggc atgttcgacg gcaagttcca atgcagggcc gtgagcggag gcaagcagtt
    13861 cggaaagagc gcgggcccgg gagacgacgt ggaaaaggaa gggacttcac ccgaagccgc
    13921 cccaggcggc ctcatgccgg gccccgacgc gggccaagcc gttcccgtcg atcctaacgc
    13981 atgccaaggc ggcatggtcg gcacgccgcc caactgccaa tgtccggaga attcggaatt
    14041 gctgggcggc aattgcgtcc gctacaccgc ttcggcatgc agcaacgggc tcgccgtgga
    14101 cgccctcccg caagcctgcc gcggcgtcga ggaaaaactc tcctgcaaac tgcgcgagga
    14161 cggactgaag gactgctgct gcgtcaccta caacaggttc taggactcga tttgtccggt
    14221 ccctaatcct ggtgcgggcc gagcggctgc ccgtggctga actcgatgat atggccggaa
    14281 ggatccttga ccgcgcagag atagccggtc ggcggcgcgt attcgcgcgg ctcccagtgg
    14341 aggcagccgt cagcgcgggc gcgctcggca atccggtcga cgtcctcacg cgccggcacg
    14401 ccgaacccgt aatgggtcat gtcaccgtcc tcctgggcat gcgccggtcc gccgccgagt
    14461 aggaccagca cgaagttgcg ttccgcgccc gggcttgcga gccagaccgt gcggctattc
    14521 cccttgccgt gctcgttgac aagctcgagc ccgcaatagc tgcgatagaa ggcaatcgac
    14581 cggtcgagat cgcgcacatg cagggcgcaa tgggtgaagg cgggtttcat gggcctcgtc
    14641 aggccccctg ccccttgccg tgctcggggt agagcgcggc gagtgcgtcg cgggtcgccg
    14701 gcgctacacc acgctcggtg atgaagccgg tcacgaggcg cgccggcgtg acgtcgaagc
    14761 cgtaatttgc agcttgggtt tccggtggcg cgatctcgac ggtggcgacg cgcccgtccg
    14821 gcaggcgccc cgcgatccgc gtcacttctt cggcggcgcg ctcctcgatg gggatcgtgc
    14881 tgccatcggc gagagtccag tcgatggtcg cgtaaggcag cgccacgaag aacgggacct
    14941 ggttgtcgcg cgcggcgagc gccttgagat acgtgccaat cttgttggcg acgtcgccgg
    15001 tcgcggtggt gcggtcggtg ccgacgatgc agagatcgac gcgccgcttc tgcatcagat
    15061 ggccgccggc attgtcggcg atgagcgtgt gcggcacgcc atgcgcgccg agctcgaagg
    15121 cggtgagcgc ggcgccctgg ttgcgggggc gggtctcgtc cacccacacg tgcacgccga
    15181 tgccttcgtc atgcgcgcga tagatgggcg caagcgccgt tccccagtcg acgcaagcaa
    15241 gccacccggc attgcaatgg gtgaggatat tgacggtcgc cccgcccttc ttctcggcat
    15301 gggccttgat gatcccgagc ccgtgctcgc cgatcttccg gttggtctcg acatcctcct
    15361 cgcagattgc cgcggcgcgg gcgaacgccg cggcgacgcg cttgtcgcgc ggctggttgc
    15421 gcacggcctt ctgcatctcg tcgagcgccc agcggagatt gatggcggtg gggcgggtgg
    15481 ccgcgagcgt ggcgcaggcg cgatcgagcg cctggtcgga ggcatcgtcg cggagcgcga
    15541 gcgcaacgcc gtaagcggcg gttgccccga tcagcggtgc tccccgtacc tgcattgtgg
    15601 caatcgcgcg cgccgcatcg tcgagggtgc ggagcgtcac cgtctcgaac acatgcggca
    15661 gcttggtctg atcgatgatt tcgaccgact tgccgtcggc gttcagccag atggtgcggt
    15721 aagacttgcc gccaatcttc atgggcgagg atttagcaca gcgcgagcag caagagcagt
    15781 gcccagcgac aatcttgcat tcctccctcg atgcgagcga tgcatcccct tcgcgtcacc
    15841 ccggacagac aacaccgata tcggcgttcg cgcaggaatc cctaggcgcc cgcgaagctc
    15901 atcagcgatt gacactcgac tccgagacgc gcgagccgcg tcgagccgcc gagatcgggc
    15961 aggtcgatga cgaaagtggc acccaccacg tcggccttga gcttgcgcaa cagcctcacc
    16021 gccgcttccg ccgttccgcc cgtggcgatg agatcgtcga cgagaagcac cggctcgccc
    16081 gctttgatgg cgtcctcgtg gacttcgatg acgtcgattc cgtattcgag ctcgtagtcc
    16141 tgtccgatag ccttggccgg gagtttgccc ttcttgcgga tcggcacgaa gccgcaatgg
    16201 agccggtccg cgacggcgcc gcccaggatg aaaccgcgtg cctcgatgcc ggcgaccgac
    16261 tgcactctcc gatcggtgaa gggcctgacc agttgctcga tgcaggcctt gaaaccattg
    16321 gcgtggccaa ggagcgtggt gatgtcgcgg aacatgatgc cgggcttcgg gtagtccggt
    16381 atggtgcgca cgaggtcttt gagattgaaa tcggccgaca cgttcaccgg gggacctcct
    16441 gacaaatgct ctcggcgaga gcgcgagccc ttgaggctat agtcgaaccc ggagcggctg
    16501 gctagtcgag gggtcgaggc ccggccgtgg ttcgaggccc gaccgtcctt cgaggctcgg
    16561 gccttgccct cgcacctcag gatgacgagc ccgctcaatt cacctcatcc tgaggaggcg
    16621 ccgaaggcgg ccctcgaagg atcggcggct gccggctcac caccccgccg cccggcttca
    16681 tcctttcagc acgcgccccg ccaccgcgtc gagctttctg aggagtgcgg gatcgcgcgc
    16741 ctcgggcgcg gtgatgatgg cggtgtcgag cgcgcggtcc gagccgatgg ggcaaggctc
    16801 gtgctcccgg ggaaaactcc gtgccagccg tgcgatgagc tgcgccccct tctcggcatt
    16861 cgccatcagc accttgatga tgtcggtgac ggtgaccgcg tcatggtcgg gatgccagca
    16921 gtcgtaatcg gtcaccatgg cgacggtcgc gtagcagatc tccgcctcac gggcgagctt
    16981 ggcctcgggc agattggtca tgccgatgac ggaatagccg agtgtcttgt aggtgaggct
    17041 ttcggcgagc gtcgagaatt gcggaccctc gatgcagaca taggtgccgc tcctcaccac
    17101 cgcgatccct tcttgttcgg cggcctcggc caggtgcagg cggagccgcg gcgacacggg
    17161 gtgggccatc gatatatggg ccacgcagcc tctgccgaag aaggagttgt gccggtgata
    17221 ggtgcggtcc acgaactgat cgacgaggac gaaggtgccg ggcgggagtt cctccttgaa
    17281 ggagccgcag gccgacagcg agacgaggtc ggtcacgccc gcccgcttga gcacatcgat
    17341 attggcccgg tagttgatgt cgctcggcga cagccggtga cccttgtcgt ggcgcgacag
    17401 aaacacgatt ggcagcccgt cgatctcgcc gatcctaagc ggcgcggaag gctcgcccca
    17461 ggggctctcg acaggttcct cgcgcacatt cttgagcccc ggcaggtcgt agagaccaga
    17521 cccgccaatc accccgagca ccgctttgac cattcacgct cctatcggga gatattctat
    17581 cgcgcggaga aactcctacg ccgcgcgctc tttttcaccg tggtggagcc gggcccatac
    17641 cgaacgcttg gcgagataca tgatcactac gaacgcgagg aggaagatca tcacccgcgt
    17701 gccgaccgca tggcgttctt cgagctttgg ctcggcagcc cacatcagaa aggcggagac
    17761 gtcccgggcg tagttgtcga cggtgggctt ggtgccgtcg gtataggtga cggttccgtc
    17821 cctcaaggga ggcgtcatgg cgatctggtg cccggggaag gccgcattgt agtgcatgcc
    17881 ctgggccagc tcgaagccct gcggcgccgg gctatagccg gtgagcagcg catagatata
    17941 gtcgtgtccg cccggccgcg ccttggcgat gaccgaaaga tcgggcggca acgcgccgtt
    18001 gttggttgcg cgcgccaatt gctcgttggc gaaaggcgat cggaaccgat ccgagggacg
    18061 cgccggacgc tggaacatct cgcccttgtc gttgggtccg tcggtcacct gaacctggga
    18121 ggcgagtatc tccacggctt cgggcgagaa ttcgggtccg ccgcgctcgc cgagagtgcg
    18181 ataggacagg agccgcatcg aatggcagtt ggcgcaaacg gtctgataca ccttgaagcc
    18241 gcgctgaagc tgggcgttgt cataagtgcc gaagggtgga tcgaagctcc agctctgacg
    18301 cgcgatttca ggctcatgat tattgctcga cgtgccggac gcgtcctcgg atgcgctcgg
    18361 gcccgcgcac gccaatagca gcgcgccggc agcggcaagg cggctgaagg aagatcgcat
    18421 cagccgaccg tctccggttt ccgtttgtgg ggcgtcctgg ccggcgatgc ccgctctgcc
    18481 ttgggcccaa gcacgggttc tgtgatgctc cccggcagcg cgcgcggctt ttcgatccgg
    18541 ccgaccacgg gcatgaccac caggaaaaag gcgaagtaat aggcggtgag gatgcggccg
    18601 aggacgaggt acagaccttc gggcgcttgc gcgccgagat agccaagcgc gacgcatgtc
    18661 accacgaagg cccagaagaa ccacttgtag atcgggcggt agcgggtcga tcgcactttc
    18721 gatgtgtcga gccagggcac taagaacaga atgaggattg agccattcag cgcggcgacg
    18781 cccaacagct tgttcgggat ggcgcgcagg atggcgtaga acggcaggaa ataccattcc
    18841 ggcacgatat gcgtcggggt ctggagcggg ttggccggga tgtagttgtc tgggtgtccg
    18901 aggaaattcg gcgcgtagaa caccaaggcc gcaaacagaa gaatgaaggc gaccagcgcg
    18961 aagccgtcct tggttgtgta gtagggatgg aacggcaccg tgtccctcgt cgatttcacg
    19021 tcgatcccgg tgggattgtt gttgcccgga atgtgcagcg cccagatatg caggacgacc
    19081 acgccggcga tcaggaagga aaacagatag tgcagggcgt agaggcgatt gagcgtcggc
    19141 tcgcccaccg cgaacccacc ccagatccac tccacgagcg tcgtgccgag accggggatc
    19201 accgaatcga gcgacgagaa gaggttggtg atcacggtga ccgcccagaa gctcatctgc
    19261 ccccacggca acgagtatcc cataaaggcg gtcgcgatca tcagcatgag gatgagcacg
    19321 ccgagaatcc acagcacctc gcgcggcgcc ttgtacgagc cgtaatagag gccgcggaag
    19381 gtgtgaatat agacggcgag gaagaacatc gaggcgccga cggcatggga atagcgcaag
    19441 agccaccccc aatttacgtc gcgcatgatt ttctcgaccg aggcgaaggc accggcctcg
    19501 gtggggacgt agtgcatggc gagcaccacg ccggtggcga tctggaccgc cagcatgaag
    19561 gtcaggatgc cgccgaaggt ccaccagtaa ttgagattac gcggcgtcgg gaagaccatg
    19621 aactggtcgt gcaccacccg cacgaccggg agccgctcat ccagccagcg ttcgataccg
    19681 gattttggct ggtagctcga gggatgcgcc atggctcgct ctagccgatc ttgagcttct
    19741 gctcggagac gaagctgtag ggcggaatgg ccaggttctc cggtgcgggt ccaatgcgca
    19801 cgcggcccgc tgtgtcgtat tgcgagccgt ggcagggaca gaaccagccg ccgtactcgc
    19861 ccttgtagtc gccgaccgcc tgccccttgg ggatacagcc gagatgagtg cagacgccga
    19921 ccatcacgag ccagggctcg cgatccttcg ccgcgcggtt atcgtcggtg gccggggcgt
    19981 ggtcgggcag attggcgttc cgcgccaagg gatcgatcag atcgtcgagc ggcgtgtcct
    20041 tggccttgac gatctcctgc tcggtgcgat ggcggatgaa gatcggcttg ccgcgccaca
    20101 tcaccgtcag cgcctggccg ggctcgatag gagagatatc gacttcggtg gtcgcgagcg
    20161 agagtgtcga agcgtcggga ttcatctgat cgagcagcgg ccagagcgcg atcgccccgc
    20221 cgaccgctgc gaagcccccg gccaccacca acagcacgtc gcgccgggtc ggctcatcgt
    20281 cgtcagggga agcgtgtacg ggaatgtcgt gactggccac aggcccaaca gctcctggga
    20341 aggacgcgat ttagacgttc aaggctttca ggcgaaatct gggagacgac aaaggcgacc
    20401 gccagggcct gcgtccttcg gctcaccggt ccaacgcccg ttcaagttgc ttcattatgc
    20461 cgcgaaccgg cgttctcggc gctggtttct tacacccgtc attattgttg tccagggggc
    20521 ctctttggac attttggcgc atgacgccgc cgccacgcgg gtccgggaca gaggtccgga
    20581 aactcggatt gccccgctct caggcaaact gccgacggcg ttttcacgcc atgccattga
    20641 cctctcggtc cgcgcggcag taaggccaag gagggatgga ccagaaggcc tcgaaacagc
    20701 cgacatcgcc ggagcccttc agtccggcgc gcaaggagcg cgcccggttg tggttcgagc
    20761 atttgcgcga cgagatctgc gccgcgctcg aggccgtcga ggatgcggcc gaaggtcttc
    20821 ccggctgcga aggcaaagcc ccgggccgct tcgtcaagac cccctggacc cgaaccgaga
    20881 aggacgggag tgacggcggc ggcggcgcca tggccctgat gtcgggccgc gtgttcgaga
    20941 aggtcggctg ccacacctcg accgtccatg ggaccctcgc gcccgagttc gccaagcaga
    21001 ttccgggcgc ggaagccgac ccgcgcttct gggcgagcgg catctccttc atcggccacc
    21061 ccgtgaaccc caacgcgccg gccgcacata tgaacacgcg catggtggtg acgtcgaagg
    21121 gatggttcgg cggcggcggc gatctcacgc ccgtgctcat gcgccgccgc acgcaagacg
    21181 atgaggactc gcgcgatttc catgcggcga tgaaagcggc gtgcgacggt gaggcctccg
    21241 ccgactacga tcgcttcaag aagtggtgcg atgaatactt ttatctgccg caccggggtg
    21301 aaatgcgggg catcggcggc atcttctacg actatctcga caccggcgac tggaaggccg
    21361 atttcgactt cacccagaaa gtcggccgcg ccttcctcgg catctatccc gagattgtcc
    21421 ggcgcaatat cggaacgcca tggacggaag ccgaccgcga cgagcagctc gtccggcgcg
    21481 gccgctatgt cgagtacaat ctgctctacg accgcggcac cctcttcggc ttgaagaccg
    21541 gcggcaatgt cgaggcgatc ctctcctccc taccgcctct cgtccgctgg ccttaagctg
    21601 gcctgagaaa cacccccagg cgtccggcct aaactcgggt attgcttccc cgcacgtcag
    21661 cttgctgccc gcgggaggaa cgatgctacg tgcgttgatc gaacagggat tgcggcgttt
    21721 gctcgccgaa aaggcgcgcg gccggacctt ccggctgcgc aaggcaagcg tcaatggacg
    21781 cggcctgcac cccgacatgc aaggcgccac ctgggaacag gtttcgcgat aagatttatg
    21841 agggtcgcgg cgggtgatcg ccgtcgatac caatccgctc ttgcgctgaa tgtcccggat
    21901 tgtttttgtc ggaatgacac gccccctatc ccctgatgcc ggcgtacatg agagggggcg
    21961 caacgggagc gggcgccgtg gtgccgatca gttcgcccac gcgggtctgg atcacggggg
    22021 cgtcgtaaac gaggcgggcc atgaggatcg caagcgccgt cagcagcaga attacaacga
    22081 tcttccccat ttcgcgatcc ctccgagcac cccgttcgac gcggcgtcgc gccgtccggt
    22141 tcaaagctct agggcttcgc ggctgaaccc tgcctgaggc cgccgttcat ctccggttta
    22201 ggtttcggct aaccgtttgt gatcgccggg ggccacttcg ggcttgccaa gcggcgcggc
    22261 ttcccccatg ctttatgtta ccgggcgccc aaggctttgg gcgcggactt ggacgacggc
    22321 aagtgactat cgcgcctcaa gagcacgacc tttcctgaca tctcaagacg ccgaaggatc
    22381 ccctttcggg ggcctgcggc ccgtttgtgt tcaacttcaa cgggaaagga acttccccta
    22441 tggctcgtgt caacggcaca gtgaaattct tcaatcatgc gcgcggcttc ggcttcattc
    22501 agcctgaaga cgggggcaag gacgtattcg tgcacgcttc cgctcttgag cggtcgggcg
    22561 tgcccgccct caacgagggc gacaaggtct ccttcgagat cgaggacgac cgccgcggcc
    22621 gcggcaagca ggccgcgaac gttcagctcg ccaactagcg aacgcgcagc gacgattgag
    22681 accgccgggc ctggtgcccg gcggtttttt tgtgccctcc cgctcgaggc ccggccgtgg
    22741 ttcgaggccg ggcccctcac catgacggtg ctttgagggc gtcatcctga ggtgcgaggg
    22801 cgatgcaacg agcctcgaag gacgcttaac gggcgattca ttctggccgc cctatccttc
    22861 caacgattga gcgccgatcg ctcgcttccg gtccgctgaa gggcacttcg cgggcactca
    22921 aggccaaaca cgcgggggaa agatcaatgg acgacggaac ccttaaagcc atcgccgcct
    22981 cggcgctgat cggtgcggcg ttacttggcg ccccggcccc ggcccaagct gggaatggcg
    23041 atgcctgggg cgcgggccta ttcggcttcg gcgtcggcgc catcatcggc agcgcacttg
    23101 cgccgcggga ggtctacgtc gtgccgccgc ccccgccggc ctattattat ggccccgttg
    23161 cgtatggccc gccgccatgg acgccggact ggtatgccta ttgctcatcg cgctaccaga
    23221 gcttcgatcc cagcaccggc tatttcatgg gctatgacgg ctaccctcat ttgtgccggt
    23281 agctgacccc gactc
//
LOCUS       DASUQP010000062        18930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1527351, whole genome shotgun sequence.
ACCESSION   DASUQP010000062 DASUQP010000000
VERSION     DASUQP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18930
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527351"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /gene="cobG"
                     /locus_tag="VFY74_02885"
     CDS             complement(<1..1105)
                     /gene="cobG"
                     /locus_tag="VFY74_02885"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: protein motif:HMM:TIGR02435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HEX5930686.1"
                     /translation="MTGVHVRGACPSLPAPMETGDGLLVRLVVNTPIPLDAFIGLCAA
                     ARTHGNGTIEVSARGSLQVRGLTPLSAPHFADAIAALDIDISEGVPVLADPLPDDPSA
                     MIDTETLAGRLREAIAKAGLTLAPKASVVVDGGGRIVLDALAADVRLRAVATGEGPRF
                     ELALAGDAASAVPVATVAAGEAVDEVLARLKAIAALGPEARAADLPQTRACRLSHRAA
                     PRQFSRADPIGLHPLGGGAIALGLGLAFGHAEAGDLMELARNATANGAEWARPAPGRA
                     LLFGPLVEVEASAARQAAERLGFVTEASDPRRRIAACPGAPSCAHGLIAARALAAEIA
                     RHVSLPGDGIALHISGCAKGCAHPKTAPLTIVGT"
     gene            complement(1102..4452)
                     /gene="cobN"
                     /locus_tag="VFY74_02890"
     CDS             complement(1102..4452)
                     /gene="cobN"
                     /locus_tag="VFY74_02890"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX5930687.1"
                     /translation="MHLLQTTSATLDDIAEPRDLGQPPGDIVILSFADSDLAGLAAAY
                     AAEKDRLPRVRLAHLRDLRHPMSIDLWIDKVARHARIVVVRLLGGLDWWRYGIEALSA
                     MARERDIGLAVLPGEDRDDPRLASASTLPPDELAELLRFFREGGRENLRALLRRLAGH
                     AGFGQDTPQAETVPRVGGYVPGRGVVALDALAAELMPGRAVVPIIFYRAALLAADTAP
                     VDALCAALSARGLAPAPLMITSLKDPEAAAFVRGALERLKPAVVVTTTAFATCGEGEA
                     SPLDAMDAPVLQVVIATTRREAWRESPRGLGPADLAMHVVLPELDGRVLAGAIAFKDA
                     LPKDEALCFAAFASRPEPDRVAMVADRIAALVKLRQTKRAERRIAVLMPDYPAAGGRA
                     GYAVGLDVPASVAALLADLAEAGYTVAHAPRTPSALLEALEAGARTPRISLLDYRRHL
                     AALPDEISGRIEAAWGAAEDDPDCRDGAFSFRAADFGTVMVALPPERGHLSDRRATYH
                     DANLPPRHAVLAFGLWLRHEACADAIVHMGAHGTLEWLPGKAVALTSACFPEAVVGPL
                     PVIYPFIVSNPGEAAQAKRRIAAVTIGHLPPPLANTSLSGAAQELERLVDEYAMADGL
                     DRRRRERLARLIIETAQRTGLATEAGLAGDLHADEALRKIDAWLCDLKDLALKDGLHI
                     YGRAPEADTSDPLWQASVEAERRALLVALDGARVAPGPAGAPQRGRRDVLPTGRNLFT
                     ADPRLLPTPTAMELGRLAADDVIRLYLQTHGEMPRSLVIDLWGSATLRTGGEEIAQGL
                     ALMGCRPSWDKATGRVTGIEVLPAGVMGRPRVDVTWRISGLFRDLFPAQIALIDAAVR
                     LLAEREEDGDNPLAAAARGARSNGGGSLARIFGAAPGAYGAGVEELLGGDSDRDAIGA
                     AYLAATSHAYSGAEGEGAAQPGAFAARVAAADLLLHGSDDPSRDLLEGAEDAAHIGGF
                     AAAAASLGREADLIVLDTTDPQRPRARPLHAALARIVRGRAVNPRFIAGMMRHGARGA
                     AELAETVDRLVDFAETTGTVTSALFDLVHEAYLADARVRAFLLRENPQAARAISERLG
                     TARRKGLWHPRRNDLDLERSAFRAEAAS"
     gene            complement(4456..5496)
                     /gene="cobW"
                     /locus_tag="VFY74_02895"
     CDS             complement(4456..5496)
                     /gene="cobW"
                     /locus_tag="VFY74_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HEX5930688.1"
                     /translation="MSASSLQRIPCTIVTGFLGSGKTTLIRHVLANANGRRLAVIVNE
                     FGDVGIDGEILKGCGDSACPEENIVELANGCLCCTVADEFVPALDQILAREPQVEHIV
                     IETSGLALPKPLVQAFHWPAIKSRVTVDGVVAVVDGAALAEGQVAADLAALSAQRSAD
                     PALDHDDPIEEVFEDQIACADLVVLNKRDLIDASGIGKAVAAIEGAPPRAVRIVTVAE
                     GRVDPSALLGLGVGTEDDIANRRTRHDSEPEHDHDDFESFVVALPELIDPAPLVARIA
                     RAGEDHKVLRIKGFAAVKEKPMRLLIQAVGARVSHYYDRPWGDAETRQGNLVVIGLKG
                     LDRDAVAKTLLG"
     gene            complement(5493..5921)
                     /locus_tag="VFY74_02900"
     CDS             complement(5493..5921)
                     /locus_tag="VFY74_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 family protein"
                     /protein_id="HEX5930689.1"
                     /translation="MALSDIAEGEDQDIAAAAATTIYVCITCRRQGEPEIDPTPGLRL
                     ARATSRAAEGTGVAVRRVRCLANCNRGLSAAIRRDGAWTYVFGGLDPDRDAAALVEGA
                     RLFAAAREAIMPSRGRPEILKRGLIARVPPIDFEEEEEGE"
     regulatory      complement(6035..6228)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      6293..6498
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6533..7162
                     /locus_tag="VFY74_02905"
     CDS             6533..7162
                     /locus_tag="VFY74_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5930690.1"
                     /translation="MNFASLRRTAFLAIPALLLTLAPASAHHAMGGQLPSTFAQGLLS
                     GLGHPVIGPDHLAFLIAVGVAVGAGGLSLALPFVFVAASAIGVALHVSGIALPGAELI
                     VAASVIVAGILLAHGRALPLTVWAALFGLAGIAHGYAYGESIFGAEATPLLAYLLGLV
                     AIQSALAVGVALIARRLGADVSAIAPRLAGAAIAGVGIMALMGQIVPGA"
     gene            7170..7706
                     /gene="cobU"
                     /locus_tag="VFY74_02910"
     CDS             7170..7706
                     /gene="cobU"
                     /locus_tag="VFY74_02910"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX5930691.1"
                     /translation="MPKPSDGGSHLTLVLGGARSGKSRYAESLIAAEPPPWIYVATAE
                     AGDDEMANRIAAHRARRDNRWQTVEAPNDLAAALAAVPARAPVLVDCLTLWLSNRLLA
                     EADIDREIEALERTLAGHDGVMVLVSNDVGSGIVPDNALARRFRDLQGALNQRIAAKA
                     SRVVLMVAGLPLFVKGMP"
     gene            7706..8320
                     /gene="cobO"
                     /locus_tag="VFY74_02915"
     CDS             7706..8320
                     /gene="cobO"
                     /locus_tag="VFY74_02915"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5930692.1"
                     /translation="MKNIDASEAERHRAKMAKRKEVQDKEVAGKTIEKGLLIVHTGTG
                     KGKTTAAFGLALRMLGRGRRVGVVQFVKGAWHTAERDALLKFGDLVSWHAMGEGFTWE
                     TQDRARDIAAAERAWEKARELIADQSFSLIVLDELNIALRYDYLDLKQVLDVLNARRA
                     DLHIIVTGRNAKPELIAAADLVTEMTLVKHHFAAGVKAQEGIEF"
     gene            8324..9784
                     /locus_tag="VFY74_02920"
     CDS             8324..9784
                     /locus_tag="VFY74_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX5930693.1"
                     /translation="MTARVLMFQGTGSDVGKSLIVAGLARALVARGLKVLPFKPQNMS
                     NNAAVTEDGGEIGRAQALQARAARVPPSVHMNPVLLKPQSEIGAQIVVQGRVFGRAIA
                     AEYQRIKPDLMPFVMDSFARVKNGADIILVEGAGSASEVNLRHNDIANMGFARAAEAP
                     VVLIGDIDRGGVIASLVGTKAVIDADDAKLIRGFLVNKFRGDPALFSDGMARIEAATG
                     WEALGLVPSFPEARLLPAEDALALDAPRQAKPGARLKIAVPILPHIANFDDLDPLDAE
                     PAVDLVRVWPGKALPGDADLVILPGSKATIADIAALRSAGFDIDIAAHLRRGGMVLGL
                     CGGYQMLGRALHDPKGIEGPAASAPGLGLLDVETTLSGEKRLEPASGMTADGIPFSGY
                     EMHMGATEGLDRGRPFARLAEGSPEGAVSADGRVIGTYIHGLFADDRQRSAWLKRFEA
                     GGAAIAYEALIEETLDKLADHLAANVAIDRLLTIAR"
     gene            complement(9768..10730)
                     /gene="cbiB"
                     /locus_tag="VFY74_02925"
     CDS             complement(9768..10730)
                     /gene="cbiB"
                     /locus_tag="VFY74_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX5930694.1"
                     /translation="MSAPLTLLALLIELTFGYPESLSRSIGHPVTWIGRLISWLEHRL
                     NRGTNAEARRRAGAVTMLVLIGVVGVASFLIETGLLLLPFGLIALALVASTLLAQRSL
                     FVHVASVADALDLGGVAAGREAVAHLVGRDTAELDEAGVARAAIESLAENFSDGAVAP
                     AFWMVLAGLPGAALYKAINTADSMIGHRSERYRDFGRTAAQLDDLVNLPASRLSALLI
                     IAAAYLTRGASAFAAWAAMRRDAAHHESPNAGYPEAAMAGALGLSLGGPRSYEGAAQE
                     GAIMGEGRREATAADIRAALDLYSRADGLLIAIVLVLAALTALW"
     gene            complement(10825..11046)
                     /locus_tag="VFY74_02930"
     CDS             complement(10825..11046)
                     /locus_tag="VFY74_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930695.1"
                     /translation="MAAESLVQMLEEIAVGLKAGDEEIYGWLRDQLKECGGSEISVEL
                     AMGVIAKLLPEEAQGNLEVARAHARRWAH"
     gene            complement(11194..11973)
                     /locus_tag="VFY74_02935"
     CDS             complement(11194..11973)
                     /locus_tag="VFY74_02935"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX5930696.1"
                     /translation="MGARSLYLVTGLTVAVSVAALVGCAGRGTFNMGQRAPWRGEVER
                     ACLASGAIQASAYVQPMSEIDGPGVCGLERPLRVSGLSGGRVWVTPAATVGCPLTAGL
                     DRWIRSSVQPAAYRYYGRPVVAITQMASYGCRGRNGNSFGAPSEHAFGNALDIAGFRL
                     AGGAEISVVQGWWRGGPRERAFLQAVIAGACPHFYTVLGPGSDRHHYNHIHVDLLLTN
                     RGRRRMCQPPIDPNVPVAEAGSEPHSTASVRPLSFVGQGAH"
     gene            complement(12223..12861)
                     /locus_tag="VFY74_02940"
     CDS             complement(12223..12861)
                     /locus_tag="VFY74_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930697.1"
                     /translation="MSAGEAWPWLLLIGLGAFHGLNPAMGWLFAVALGLYRRSRHAVL
                     VSLIPIALGHAAAIAVVVYAAIALGLAIDQGAFRIVAGLILIAWGAYHAIYGHRHRLR
                     IGLTTGLLGLAFWSFTMATVHGAGMMLIPVLMPLAQLSEHARHMPVATSLGVAALAVL
                     VHSLAMLLTTGAIALAVYDWIGLDFLRRGWINLDLVWSAALIGMGLWLLLVP"
     gene            complement(12868..14259)
                     /locus_tag="VFY74_02945"
     CDS             complement(12868..14259)
                     /locus_tag="VFY74_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding family protein"
                     /protein_id="HEX5930698.1"
                     /translation="MTRWTPDPTFYPSPRLAAKAPAETLAYVAAFAPERDVPDALAVV
                     DVDPASPTYSKIVGGVDMPNVGDELHHYGWNACSSCLCPNAPHPHTERRYLVVPGLRS
                     SRIHIIDTKQDPKSPKIVRVIEAEELIEATGYSRPHTVHCGPEGIYLSALGDAKGETP
                     GGIFLMDAETFDVLGRWEVDRGPQRLAYDAWWHLGYDTLVTSEWGAPNTVENGLVPEI
                     LLGAQYGRRLHFWDLHKRKHLQEIDFGPDYQLVFELRPAHDPTKAYGFVNCVVSLKDL
                     SSSIWTWYRDGETWAVKKVIEIPAEPADPDLLPPLLKGFKAVPPLVTDIDLSMDDRFL
                     YVSCWGTGDLRQYDVSDPFDPKLAGTVRIGGIVARAPHPRANGPLNGGPQMVEISRDG
                     RRVYFTNSLYGAVDPQFYPEGIDGWMVKLDAGENGGIDFDPRFFVAWPKSHRPHQVRL
                     EGGDCSSDSYCYP"
     gene            complement(14332..15528)
                     /locus_tag="VFY74_02950"
     CDS             complement(14332..15528)
                     /locus_tag="VFY74_02950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5930699.1"
                     /translation="MFTWSLIAAATVAFGLASAVKAETEQAEVVATPLVATPIAPPNP
                     VLGSDGKIHLAYEIVLMNMAPSAVTVEKIETLDAHSGAAIGTLEGEGLAKMTRLNGGA
                     KGTALAAGGSGFVFMEVTLAKDATLPKALRHRFAIGVATAPKQEEKGDRDPAPAPPRE
                     LTFVTDPLAVGPGAVMIAPPLKGSRWVMGGGCCTPYSYHRGATLPIDGAVRVAERFAI
                     DFVQLNDNDQLNSGPVDQLSSYAYLGDEIYAVADGTVVGMEDGLPEQVPGKLPEDATI
                     QTAAGNHVVVDIGTDRFALYAHMQPGSVRVKVGDTVTKGQVLGLLGNSGNTDTPHLHF
                     HIMDGPSPLVSNGLPYVFTSFTGQGRLTDEQPLFTGGRVTIDKEALSGPHENAYPLID
                     QVVSFP"
     gene            complement(15604..17565)
                     /gene="ligD"
                     /locus_tag="VFY74_02955"
     CDS             complement(15604..17565)
                     /gene="ligD"
                     /locus_tag="VFY74_02955"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX5930700.1"
                     /translation="MDLEGSKKNSGGAREAQGRGKAKAKTRRALGPDFVAPELATLES
                     EVPTGPGWFHEIKYDGYRIIGRKVGDEITLFSRSGLDWTVRFPAIAKALLTFPAETVL
                     LDGEIAFVLPSGVTDFKSLQEHIDSPHGSIRYFLFDLLSLDGKDWRKKPLKDRREKLA
                     GLMAAKGVSNFLVYADHVEGSGSEFLAQACAAGLEGIVSKRADRPYLSGRGKDWLKIK
                     CKRGDEFVIGGYSRSDVRGKPFSSLLLGEFSGVELVYAGKVGTGFDAADFRNLSRRFK
                     PLERATSAFVEVPAPERKGAVWLEPKLVCQVTYAEKTRDGRLRHPSFQGLREDKPVRE
                     VHREQTDAEETSMAKASARGRDGATFDGITLTNPDRVLFPDIDLTKFDLARYYEAVAS
                     FMLPYVLRRPISLVRCPEGYTKECFFQRHAMKGMSDTIKEVPIAGGETKKKYLYIDDE
                     AGLFALVQIGVLEIHDWGVSLGHIDKPDRIVFDLDPDEDLDLATLKASAVEVRDFLGD
                     LGFKSFLKSTGGKGLHLVAPLTPKLGWDEVKAFTKAIADALVSARPDRYTANPLKKTR
                     EGKIFVDYLRNQRGGSAIVNYSTRAKKGAPVSCPLRWDELKALKAAAPYTAKTLPARL
                     KALKGDPWDGFFATRQSISAKARKALGLG"
     gene            complement(17475..18173)
                     /locus_tag="VFY74_02960"
     CDS             complement(17475..18173)
                     /locus_tag="VFY74_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5930701.1"
                     /translation="MARAIRGGAGGRDLLATYKAKRDFAKTPEPKAKIKRASGNSFVI
                     QKHAARRTHFDFRLEHDGVLKSWAVTRGPSLDPSQKRLAVMTEDHPLEYGGFEGVIPK
                     GEYGGGPVMIWDEGTWDPLGDPDEGLAKGDLKFRLNGKRLKGDFVLVRMKPRKEDRGR
                     QNWLLIKKRDAYAGDGNEPTQIYDTSAISGRTMDQIQSGDVPVWTSKAQKKTPAARVR
                     RKAAAKPKRRPAAR"
     gene            complement(18178..>18930)
                     /locus_tag="VFY74_02965"
     CDS             complement(18178..>18930)
                     /locus_tag="VFY74_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5930702.1"
                     /translation="KRIRYQKVVPGVGPVENEDITKGYEYEKGKYVLIDPQEIDELKL
                     EAKQTIELVRFVDDEVIDARYYETPYYLLPDGDMADEGYSIIQKALKDTGKVGVGQFI
                     LRGRGNIVAIKPFGKGLLLEVLRHANEIRAAEPLFKEITEIKADKEALDLAKELIKRK
                     TGKFEPEALKDAYNEAVWELIQAKLEHRAPEIVTEMPGSAKVINIMDALKKSVKQGKL
                     AGAKETAKRGTASARSGNGKKAARGTPKRKSA"
BASE COUNT         3104 a         6316 c         6384 g         3126 t
ORIGIN      
        1 ctgtgccgac aatggtgagg ggcgctgtct tcggatgggc gcaccccttg gcgcatcccg
       61 agatgtggag agcgatgccg tcaccgggaa gtgacacatg ccgagcgatc tccgcggcaa
      121 gcgcgcgcgc cgcaatcaag ccatgcgcgc aagaaggcgc gcccgggcag gcggcgatgc
      181 ggcggcgcgg atcggaagcc tccgtcacga agccaaggcg ttctgcggcc tgccgcgctg
      241 ccgaagcctc tacctcgaca agcggtccga acagaagcgc gcggcctggc gcgggccgcg
      301 cccattccgc gccattggcc gttgcgtttc gggcaagctc catgaggtcg ccggcttcag
      361 cgtggccgaa agcaagcccg agcccgagcg cgattgcgcc ccctccgaga ggatggaggc
      421 cgattggatc ggcgcgcgag aattgccggg gcgctgcccg gtgagacagt cggcaggccc
      481 gtgtctgcgg caaatcggcg gcgcgggctt caggtccaag cgcggcgatg gctttgagcc
      541 gcgcgagcac ttcatcgacg gcctcgccag ccgcgacggt ggcgaccggc acggcggacg
      601 cggcatcgcc ggcaagcgcg agctcaaatc ttggcccctc gcctgttgcg accgcgcgaa
      661 gtctgacgtc tgccgcaagc gcgtcgagaa cgatgcgccc gccgccatcg acgaccaccg
      721 acgccttggg cgcaagagtg agacccgcct tggcgatggc ctcgcgaagt cggccggcaa
      781 gagtttccgt gtcgatcatc gcggagggat cgtcaggcag aggatcggcg agaaccggca
      841 cgccctcgct aatgtcgatg tcgagcgcgg caatcgcgtc cgcgaaatgt ggggcggaaa
      901 gcggcgtcag tccgcggacc tggaggctcc cccgcgcgct aacctcgatt gtgccgttgc
      961 cgtgtgtccg cgcagccgcg cagaggccga tgaaggcatc gagcggaatg ggcgtgttga
     1021 cgacgaggcg cacgagcagg ccgtcgcccg tttccatggg cgccggcaag ctcgggcagg
     1081 cgccgcgaac gtggacgccg gtcatgacgc tgcctccgcc cgaaacgcgg accgttcgag
     1141 atcgagatcg ttccggcgcg gatgccagag ccctttgcgc cgcgccgttc ccagccgctc
     1201 ggaaatggct cgcgcggctt gcgggttctc gcgcagcagg aacgcgcgaa cgcgggcgtc
     1261 ggcgagatag gcctcatgca cgagatcgaa tagcgcgctc gtgacagtgc ccgtggtctc
     1321 ggcgaaatcg acgagacgat ccacggtctc ggcaagctct gcggcgccgc gcgcgccgtg
     1381 gcgcatcatg cccgcgatga agcgcggatt gacggcgcga ccgcgcacga tcctggcaag
     1441 cgccgcgtgg agcgggcgcg cgcgcggccg ctgtggatcg gtcgtgtcga gcacgatgag
     1501 gtcggcctcg cgcccgagcg aggctgccgc cgcggcgaac ccgccgatat gggccgcgtc
     1561 ctccgcgcct tcgagcagat cgcgagatgg atcgtcgctc ccgtgcagga ggagatcggc
     1621 ggcggcgacc cgcgccgcga aagctccggg ctgcgccgcg ccctcgccct cggccccgct
     1681 ataggcatgc gaggtcgcgg caagataggc cgcgccgatc gcgtcgcgat cgctgtcccc
     1741 tccaagcagc tcctcgacgc ccgcgccgta ggcgccgggg gccgcgccga agatgcgcgc
     1801 caggctcccg cccccgttgc ttcgcgcgcc gcgcgccgcg gcggcgagcg gattgtcgcc
     1861 gtcctcctca cgctcggcga gcagtctcac cgccgcgtcg atcagcgcga tctgcgccgg
     1921 gaagaggtcg cggaacagcc ctgagatgcg ccatgtcacg tcgacgcgcg ggcgccccat
     1981 cacgcctgcg ggcaggacct cgatgccagt cacgcggccg gtcgccttgt cccagctcgg
     2041 gcggcagccc atgagcgcga ggccttgcgc gatctcctcg ccgcccgtcc tgagcgtggc
     2101 gctcccccaa agatcgatga cgagcgagcg cggcatctcg ccatgcgtct ggaggtagag
     2161 gcggatcacg tcgtccgccg cgagcctgcc gagttccatc gcggtcggtg tcggcaggag
     2221 gcgcggatcg gcggtaaaga gattgcggcc cgtcggcaga acgtcgcggc ggccgcgctg
     2281 cggggcgcca gcaggccccg gcgcgacgcg ggccccgtcg agcgcaacga ggagtgcgcg
     2341 gcgctcggcc tcgacgctcg cctgccacag tggatcgctt gtgtcggctt ccggcgcacg
     2401 cccgtagata tgcagcccgt ccttcagcgc gaggtccttc agatcgcaga gccaggcgtc
     2461 gatcttgcgc agcgcctcgt ccgcgtgaag gtcgcccgca agccctgctt ccgtcgcgag
     2521 tcccgtcctc tgagccgtct cgatgatgag ccgcgccaga cgctcgcgcc ggcggcggtc
     2581 gagcccgtcg gccattgcat attcgtcgac caggcgctcg agctcctgcg ccgcgcccga
     2641 gagcgaagtg ttggccagag gcggcggcag atgcccgatg gtgacggcgg cgatacggcg
     2701 cttggcctgt gccgcctcgc ccggattgct gacgatgaag ggatagatca cggggagcgg
     2761 gccgaccacg gcctcgggga agcaggcgct ggtgagcgcc acggccttcc ccggcagcca
     2821 ttcgagcgtg ccatgcgcgc ccatatgcac gatcgcatcg gcgcatgcct cgtggcgcag
     2881 ccatagccca aatgcgagca cggcatggcg cggcggcaaa ttggcgtcgt gataggtcgc
     2941 gcgccggtcg ctcaagtgcc cgcgttctgg cggaagcgcc accatcacgg ttccgaagtc
     3001 ggcggcgcgg aagctgaagg cgccgtcacg gcaatcaggg tcgtcctcgg ctgcgcccca
     3061 tgccgcctcg atgcggccgg atatctcgtc gggaagcgct gcgagatgcc gccggtagtc
     3121 cagcagcgag atccgaggcg ttctcgcgcc ggcctcgagc gcttcaagca atgccgaagg
     3181 tgttcgcggg gcgtgggcga ccgtgtagcc cgcttcggca agatcggcga gaagcgcggc
     3241 gacgcttgcc ggaacgtcga ggcccacggc gtagccggcg cggccgcccg cggcgggata
     3301 gtcgggcatg aggaccgcga tgcggcgctc cgcccgcttc gtctgccgga gcttcacgag
     3361 tgccgcgata cggtcggcca ccatggcgac gcggtcgggc tcgggccggc ttgcgaacgc
     3421 ggcgaagcaa agcgcctcgt ccttcggcag cgcgtccttg aaggcgatgg ccccggcgag
     3481 cacgcggccg tcgagctcgg gcagcaccac atgcatggca agatcggcgg gcccaagccc
     3541 gcgcgggctc tcccgccagg cttcgcgccg cgtcgtcgcg atcacgactt gcagcaccgg
     3601 cgcgtccatc gcatcgagcg gcgatgcctc gccctcgccg cacgtcgcga acgctgtggt
     3661 cgtgaccacg accgcgggct tgagccgctc gagcgcgccg cgaacgaacg cggcggcctc
     3721 cgggtccttg aggctcgtga tcatgagcgg tgccggcgca aggccgcggg cggaaagcgc
     3781 cgcgcagagc gcatcgacgg gcgcggtgtc ggcggcaagc agcgccgcgc ggtagaagat
     3841 gatcggcacc acggcgcggc ccggcatgag ttctgccgcg agcgcatcga gagccacgac
     3901 gcccctgccc ggcacgtagc cgccaacgcg cggcacggtc tccgcctgcg gcgtatcttg
     3961 accgaagccc gcgtgaccgg cgagccgccg gagcagcgcg cgcaaattct cgcgcccgcc
     4021 ttcgcggaag aaacggagca attcggcaag ctcatcgggc ggcagcgtcg aggcggaggc
     4081 gaggcgcgga tcgtcgcgat cctctcccgg cagcacggcg aggccgatat cgcgctcgcg
     4141 cgccatggcg gagagcgcct cgatcccgta gcgccaccaa tcaagcccgc cgagcagcct
     4201 caccacgacg atcctggcgt ggcgcgcgac cttgtcgatc cagagatcga tcgacatcgg
     4261 atggcggaga tcgcggagat gcgcgaggcg gaccctgggg agccggtcct tctccgcagc
     4321 gtaagccgcg gcaagaccgg ccagatcgct gtcggcgaag gacaggatca cgatatcgcc
     4381 cggcggctga cccaaatcgc gcggctcggc gatatcgtcg agcgtcgcgg acgtggtctg
     4441 aaggagatgc atggcctacc cgagcagagt cttggcgacg gcgtcgcggt cgagaccttt
     4501 cagcccgatg accacgagat tcccctgccg cgtctcggcg tcgccccaag gccggtcgta
     4561 atagtggctg acgcgcgctc ccaccgcctg gatcaagagg cgcatcggct tctccttcac
     4621 cgcggcaaaa ccctttatgc ggagcacctt gtggtcttcg cccgcgcgcg cgattcgcgc
     4681 gacgaggggg gcgggatcga taagctcagg cagcgcgacg acgaagctct cgaaatcgtc
     4741 gtgatcgtgc tcgggctcgg aatcgtggcg ggtccttcgg ttcgcgatat cgtcctcggt
     4801 gccgacgcca agcccgagga gggcggaggg atcgacccgc ccttccgcca ccgtgacgat
     4861 gcgcactgcg cgcggcggcg cgccctcgat ggccgcaaca gccttgccga tgccggaggc
     4921 atcgatgagg tcgcgcttgt tgagcacgac gagatcggcg caagcgatct ggtcttcgaa
     4981 cacttcttcg atgggatcgt catgatcgag cgcggggtcg gccgagcgct gggccgatag
     5041 cgcggcaagg tcggctgcga cttggccctc ggccagcgcc gcaccatcga ccacggcgac
     5101 aacgccgtcg acggtgacgc ggctcttgat ggcgggccaa tggaaggcct ggaccagggg
     5161 cttcggcaga gcgagacccg aagtctcgat gacgatgtgc tcgacttgcg gctcgcgcgc
     5221 gagaatctga tcgagcgccg gcacgaattc atccgccacg gtgcagcaca ggcagccatt
     5281 ggcgagctcg acgatattct cctcggggca ggcagagtcg ccgcagcctt tgaggatctc
     5341 gccgtcgatg ccgacatcgc cgaactcgtt cacgattacc gcgagccggc gcccattggc
     5401 gttggcgagc acgtggcgga tcagcgtcgt cttgcccgag ccgaggaagc cggtgacgat
     5461 ggtgcaaggg atacgctgaa gcgacgacgc actcattcgc cctcctcctc ctcctcgaaa
     5521 tcgatcggcg gcacacgggc gatcaacccg cgcttgagga tttcggggcg gccgcgcgac
     5581 ggcatgatcg cttcacgcgc cgcggcaaag agccgcgcgc cttcgacgag agcggcggcg
     5641 tcccggtcgg gatcgagccc gccgaatacg taggtccagg cgccgtcgcg cctgatggcg
     5701 gcgctcaagc cccgattgca gttggcaagg cagcgcaccc gccgcaccgc gacgcccgtc
     5761 ccctccgccg cgcgggacgt ggcgcgcgca agacgaaggc caggtgtcgg gtcgatctcg
     5821 ggctcgcctt ggcggcggca ggtgatgcag acatagatcg tcgtcgcggc ggccgccgcg
     5881 atgtcttgat cctcaccttc cgcgatgtcg ctgagagcca cccgaagccg ctcctcgaac
     5941 tggaatctgc cgcgaccaag ggtgccggcg gcggcctaat cctcgcttcg gaccggccgc
     6001 catcgaacca accgctccga ggcaccccgc ccggcgagcg actggaaccg acggtaggtc
     6061 tcctggctcg cgggtctcag cctttgccgc cttcccggga cgttcccagt ggctctatgg
     6121 ctctggctcg ccgcttacag ttgcgggggc agccgcggag ttgggttttg gcccttaccg
     6181 cgttccctgt tcctctcttc tagagagacc gtcaatccgg ggcacttaac ggcgaggccg
     6241 cgccctttgt caatgcgcct gaccggccct tgcgaagcgc ggcgcgcggg ctatagtcgc
     6301 gagcgtcgtc ggttcccgcg cggtgcggga cgcaaaaggg aacgcggtaa ggggttcacg
     6361 cccaaagccg cggctgcccc cgcaactgta agcggcgagc agccctcagt cagccactgg
     6421 aggcacggaa ctccgggaag gccgagggca ggctgtgacc cgcgagccag gagacctgcc
     6481 ggcggcgtca actctagccc tgccctcggg tggagggcgc aagggggatc acatgaattt
     6541 cgcatcgctt cgccgcacgg ccttcctcgc aatcccagcg ctgctgctca cgctcgcgcc
     6601 ggcatcggcg caccacgcca tgggcgggca attgccctcg actttcgctc aaggcctcct
     6661 gtcgggcctc ggtcatccgg tcatcgggcc tgaccacttg gcctttctca tcgcggtcgg
     6721 cgttgccgtc ggcgcgggtg ggctgagcct tgcgcttccc ttcgtgttcg tcgccgcctc
     6781 cgccatcggc gttgcgctgc atgtgagcgg gattgcgttg ccgggcgcgg agctgatcgt
     6841 cgcggcctcg gtgattgtcg ccggcatact cctcgcgcac gggcgcgcgc tgccgctgac
     6901 cgtttgggcg gctctcttcg gtctcgccgg catcgcgcat ggctatgcct atggcgagtc
     6961 gatcttcggc gccgaagcga cgccgctcct cgcatacctc ctcggtctcg tcgcgatcca
     7021 gagtgcgctg gcggtcggcg ttgccctgat cgcgcgccgc ctcggcgccg acgtttccgc
     7081 aatcgcgccg cgccttgccg gtgcggcaat cgcgggggtt ggaataatgg cgctcatggg
     7141 gcaaatcgta ccgggcgcgt gaccggctga tgcctaagcc aagcgacggg ggcagtcacc
     7201 tcaccctcgt cctcggcggc gcccgctccg gcaagagccg ctacgccgaa agcctgatcg
     7261 cggccgaacc gccgccatgg atctatgtcg cgacagccga agcgggcgac gacgagatgg
     7321 caaaccgcat cgccgcccat cgcgcgcgcc gcgacaatcg ctggcagacc gtcgaggcgc
     7381 cgaacgatct cgccgctgcg cttgcagccg tgcctgcgcg cgctccggtg ctggtcgatt
     7441 gcctgacgct ctggctctcc aatcgcctgc tggccgaggc cgatatcgac cgggagatcg
     7501 aggcgctcga acgtactctg gccggccatg atggcgtcat ggtcctggtt tcgaacgacg
     7561 tcggctccgg catcgtcccc gacaacgcgc ttgcgcgtcg attccgggac ctgcaagggg
     7621 cgctcaatca gcggatcgcg gccaaggctt cccgcgtggt tctcatggtc gccgggcttc
     7681 cgcttttcgt aaagggaatg ccatgatcaa gaacatcgac gcgagcgaag ccgaacgcca
     7741 tcgcgccaag atggcgaagc ggaaagaggt gcaagataaa gaggtcgccg gcaagacgat
     7801 cgagaagggc ctcctcatcg ttcacaccgg taccggcaag gggaaaacga cggcggcctt
     7861 cgggctcgcg ctccgcatgc tcggccgggg gcggcgtgtc ggcgtggtgc agttcgtcaa
     7921 gggcgcttgg catacggccg aacgcgacgc gcttttaaaa ttcggcgacc tcgtctcttg
     7981 gcacgcgatg ggcgagggct tcacgtggga gacccaggat cgcgcgcgcg acatcgccgc
     8041 cgcggagcgc gcatgggaga aagcgcgaga gctgatcgcc gatcaaagct tcagcctgat
     8101 cgttctcgac gagctcaaca tcgcgctccg ctacgactat ctcgatctga agcaagtgct
     8161 cgacgtgctc aacgcgcgcc gcgccgatct tcacatcatc gtcaccggca ggaacgccaa
     8221 gcccgagctg atcgcggccg cggacctcgt caccgagatg acgctggtga agcatcactt
     8281 cgccgccggc gtgaaggcgc aggaaggcat cgagttctga gtcgtgacgg cgcgtgtgct
     8341 catgttccaa ggcaccggct ccgatgtcgg caagtcgctg attgtcgcgg gtctcgctcg
     8401 ggcgctcgtc gcgcgcggac tcaaagttct gccgttcaag ccgcagaaca tgtcgaacaa
     8461 cgcggccgtg accgaagacg gtggtgagat cggcagggcg caggcgcttc aggcgcgcgc
     8521 ggcgcgcgtg ccgccgagcg tgcacatgaa cccggtcctg ctcaagccgc agagcgagat
     8581 cggcgcgcag atcgtggtgc aaggccgcgt cttcggacgc gccatcgccg ccgaatatca
     8641 gcggatcaaa cccgacctga tgccgttcgt gatggacagc ttcgcgcggg tgaagaacgg
     8701 cgccgacatc attctggtgg aaggcgcggg cagtgcgtcg gaagtgaacc tccgccacaa
     8761 cgacatcgcc aatatgggct tcgcccgcgc cgcggaagcg ccggtcgttc tcatcggcga
     8821 catcgaccgg ggcggcgtga tcgcgagcct cgtcggcacc aaggcagtga tcgatgccga
     8881 tgacgcgaag ctcatccgcg gctttctcgt caacaaattc cgcggcgatc ccgcgctctt
     8941 ctccgacggg atggcgcgca tagaagcggc gaccggctgg gaggctctgg ggctcgttcc
     9001 ctcctttccc gaagcgcggc tcttgccggc ggaggatgcg ctcgcgctcg acgccccccg
     9061 ccaggcgaag cccggcgcgc gcctcaagat cgctgtcccc atactgccgc atatcgccaa
     9121 tttcgatgat ctcgacccgc tcgacgccga accggccgtc gatcttgtgc gcgtgtggcc
     9181 cggcaaggcg cttcccggcg atgcggacct cgtcatactg ccgggctcca aggccaccat
     9241 cgccgatatt gccgcgctcc gctccgccgg cttcgacatc gacattgccg cgcatcttcg
     9301 ccgcggcggc atggtgctcg gactttgcgg cggctatcag atgttggggc gcgcgctcca
     9361 cgacccgaag ggaatcgagg ggcctgcggc aagcgcgccc ggtctcggcc tgctcgacgt
     9421 cgagaccacg ctatcgggcg agaagcgact cgagccggcg agcggcatga cggccgacgg
     9481 catccccttc agcggctacg agatgcatat gggcgcgacc gaaggcctcg atcgcgggcg
     9541 gccattcgcg cgcctcgccg agggctcgcc cgaaggagcg gtctcggccg atggccgcgt
     9601 catcggcacc tatatccatg ggctcttcgc cgacgaccgc cagcgctcgg cctggctcaa
     9661 gcggtttgag gcgggcggcg cggcgatcgc ttacgaggct ctcatcgagg agacgctcga
     9721 caagcttgcc gatcatcttg ccgccaatgt cgccatcgac cggcttctca ccatagcgcg
     9781 gtaagggccg cgagcacgag cacgatggcg atgagaagtc cgtcggcgcg gctgtagaga
     9841 tcgagcgcgg cgcggatgtc tgcggctgtg gcctcgcgcc gtccctcgcc catgatggcg
     9901 ccctcctgcg ccgcgccttc ataggagcgg gggccgccaa gcgaaagtcc gagagccccg
     9961 gccatcgcgg cctccgggta accggcattc ggggattcgt ggtgcgcggc gtcgcgccgc
    10021 atcgcggccc acgctgcgaa ggccgatgcg cccctggtga gataggccgc ggcgatgatg
    10081 agaagcgccg agagccgtga ggcgggaagg ttgaccaaat cgtcgagctg ggccgcagtc
    10141 cggccgaaat cgcgataccg ctcgctgcgg tggccgatca tgctgtcggc ggtgttgatc
    10201 gccttgtaga gcgcggcacc tggaagcccg gcgagcacca tccagaaagc cggcgccacg
    10261 gcgccatcgg aaaaattctc cgccagactt tcgatcgcag cccgcgcaac gccggcctcg
    10321 tcgagttcgg ccgtgtcgcg cccgaccaaa tgcgcgacgg cttcacgtcc ggcggcgacg
    10381 cctcccaaat cgagcgcgtc ggcaacggaa gcgacatgga cgaagagact gcgctgggca
    10441 aggagcgtgc tggcgacaag ggcaagcgcg atcagcccga acggcagaag gagaaggccc
    10501 gtctcgatga ggaaggaagc gacgccgacg acgccgatca gcaccagcat cgtcacggcg
    10561 ccagccctgc ggcgcgcctc ggcattggtg ccgcgattga gacgatgttc gagccacgag
    10621 atcagccggc cgatccacgt gacgggatgg ccgatcgaac gcgaaaggct ttcgggatag
    10681 ccgaacgtca gctcgatcag caaagcaagg agcgtcagcg gcgcggacat gggatctccc
    10741 gcgatcctgc gccgctcgac cgcggcgaga gagcgccctt gcgcggcgcc ccccacgcgc
    10801 gcatgcgaca gtcgggtgct atgatcagtg agcccagcgg cgtgcgtgcg ccctggccac
    10861 ttccagattt ccttgcgcct cttccggcaa gagcttcgca atcacgccca tggcaagctc
    10921 gacggagatc tcgctgccgc cgcattcctt cagctggtcg cgcagccagc catagatctc
    10981 ctcatcccca gccttgaggc cgacggcgat ctcttcaagc atctgcacca acgattctgc
    11041 tgccatcgtg cccctccctt cccccgagcc caaaggctta tggctgcggt cgcctgattc
    11101 ttgctcttgg tgcgatcctc ccctgattcg catcatactt ggtcttgcgc cgctttgacg
    11161 tggcgatccg gccacagtca gcttctattt cggtcagtgg gcgccctggc ccacaaagga
    11221 aaggggcctg accgaagcgg tgctgtgcgg ctcgctcccg gcctccgcga ccggcacgtt
    11281 ggggtcgatg ggcggctggc acattcgacg ccggcctctg ttggtgagca ggagatcgac
    11341 gtggatgtgg ttgtagtggt ggcggtcgct gcccggtccg agcacggtat agaaatgggg
    11401 gcaggcgccg gcgatcaccg cctgaaggaa ggcgcgctcc cgtgggcctc ctctccacca
    11461 gccctggacc acgctgatct cagcgccgcc ggcgagccgg aaacccgcga tatccaaggc
    11521 gttgccgaag gcgtgttccg acggggcgcc gaacgagttg ccgttccggc cgcggcaccc
    11581 gtaggaggcc atctgcgtga tcgccaccac cgggcgcccg taatagcgat aggccgcggg
    11641 ctgcaccgaa ctcctgatcc agcgatcgag cccggccgtg agcgggcagc cgacagtcgc
    11701 cgcaggcgtg acccagaccc tgccgcccga tagaccggac acgcggagcg gccgctcgag
    11761 cccgcacacg ccgggcccgt cgatctcgct catgggttgg acataggccg acgcttgaat
    11821 cgcgccgctt gccagacaag cccgctcgac ctcgccgcgc cagggcgccc tctggcccat
    11881 gttgaaggtc ccgcggccgg cgcagccgac aagagccgcc acggaaactg cgacggtcag
    11941 gcccgttacg agatacagac tccgtgcccc catggccgcc aagatgcggc caagggtctg
    12001 aaaaatgttt caatggcatt tccgttaacc ccctctcccc ttgagggaga gggcttcccg
    12061 ctcaggctac gcgaagcgcg acgaatggcg ggaagggtga ggggtgaatc ttttcgacgc
    12121 gggcttcgcc cggttacccc tcaccgcgcc gctcacgcaa cgcaagcgtt ggcgcatcaa
    12181 agacgcggcg cgtgctctca caaggggaga gccaaaggag tgtcatggca cgagcagcag
    12241 ccagagtccc atgccgatga gcgcagcgga ccagacgaga tcgagattga tccaaccccg
    12301 ccgcaagaag tcgagaccta tccagtcata gacggcgagc gcgatggcgc cggtcgtgag
    12361 cagcatggcg aggctgtgaa cgagaaccgc gagagccgcg accccgagcg acgtcgcgac
    12421 cggcatgtgg cgagcatgtt cgcttaactg cgcgagcggc atgagcacgg ggatcagcat
    12481 catgccggcg ccgtgcaccg tcgccatggt gaaggaccaa aaggcaagtc cgagcagccc
    12541 ggtggtgagc ccaatgcgca ggcggtggcg atggccgtag atggcatgat aggcgcccca
    12601 cgcgatcagg atgaggccgg caacgatgcg aaaggcgcct tggtcgatcg caagcccgag
    12661 cgcaatggcg gcatagacca caacggcgat cgccgcggcg tgaccgagcg caatgggaat
    12721 gagcgagacg aggacggcgt gacggctccg ccggtagagc ccgagcgcca cggcgaacag
    12781 ccagcccatc gccggattca atccgtgaaa ggcgccaaga ccgatcagca gcagccaagg
    12841 ccaggcttcg ccggcgctca ttcacgctca cggatagcaa tacgagtccg acgagcagtc
    12901 gcccccttcg aggcggacct gatgcgggcg gtggctcttc ggccaggcga cgaagaagcg
    12961 cgggtcgaaa tcgatgccgc cattctcgcc tgcgtcgagc ttcaccatcc agccgtcgat
    13021 tccttccgga tagaattgcg ggtcgacagc gccatagagc gagttggtga agtacacgcg
    13081 ccgtccgtcg cgactgatct cgaccatctg cggaccgccg ttgagcggac cgttcgcccg
    13141 cggatgcggc gcgcgcgcga cgatgccgcc gatcctgacc gtgccggcga gcttcgggtc
    13201 gaatgggtcg ctgacgtcgt attggcgcaa atcgccggta ccccagcacg acacgtaaag
    13261 gaagcggtcg tccatggaga ggtcgatatc ggtaacgagc ggaggcacgg ccttgaagcc
    13321 tttcagcagg ggcggcagaa gatcggggtc cgccggctcc gccggaatct cgatgacctt
    13381 cttgacagcc catgtctcgc cgtcgcgata ccaggtccag atcgaggacg acaaatcctt
    13441 gaggctcacg acgcaattga cgaagccgta agccttggtc ggatcgtgcg cgggcctgag
    13501 ctcgaacacg agctggtagt cggggccgaa atcgatctcc tgaagatgtt tgcgcttgtg
    13561 caggtcccag aagtggagcc gccgaccgta ttgcgcgcca agcaggatct ccggcacgag
    13621 cccgttttcc acggtgttgg gcgcgcccca ctcactcgtc acgagggtgt cgtagccgag
    13681 atgccaccag gcatcgtagg cgagacgctg cggtccgcga tccacttccc agcggccgag
    13741 cacgtcgaac gtttcggcat ccatcaggaa gatgccgccc ggcgtctcgc ccttggcgtc
    13801 gccgagcgcg gagagataaa tgccctcggg cccgcaatgc accgtatgcg ggcggctgta
    13861 ccccgtggct tcgatcaatt cttcggcctc gatcacccgc acgatctttg ggctcttcgg
    13921 gtcttgcttg gtgtcgatga tgtggatgcg cgaggagcgc aagcccggca cgacgagata
    13981 gcgccgctcg gtatggggat ggggcgcgtt ggggcaaaga caggagctgc acgcgttcca
    14041 gccatagtgg tgaagctcat cgcccacatt cggcatgtcg accccgccca cgatcttcga
    14101 ataggtcggc gaggcgggat caacatcgac cacggcgagc gcgtcgggga catcgcgctc
    14161 gggcgcaaac gccgcgacat aagcgagcgt ctcggccggc gccttggctg cgagccgggg
    14221 cgatgggtaa aacgtcgggt caggcgtcca tctcgtcatc gcggtgctcc ttttgccccc
    14281 gagcgccatc ttcgccgccc gccgagcgcg tggcaaggcg gggcgtggat gctacgggaa
    14341 gctcaccacc tgatcgatca ggggataggc gttctcgtgc ggccctgaca gggcctcctt
    14401 gtcgatcgtc actcggccgc ccgtgaacag aggctgctcg tcggtgaggc gcccttgtcc
    14461 ggtaaaggac gtgaacacgt aaggaagccc gttcgatacg agcggcgagg gtccatccat
    14521 gatgtgaaag tggaggtgag gcgtgtcggt attgccgcta ttgccgagga ggccgagcac
    14581 ttggcccttc gtgaccgtgt cgccgacctt cacccgcacg cttccgggct gcatatgcgc
    14641 gtagagggca aaccggtccg tgccgatatc gacgacgaca tggttgccgg cagccgtctg
    14701 gatggtcgca tcttcgggaa gcttgcctgg cacttgctcg ggcaaaccgt cctccatgcc
    14761 aaccacggtc ccgtcggcca cggcgtagat ctcgtcgccg aggtaggcgt agctcgacag
    14821 ctgatcgacg ggacctgagt tcaactgatc gttgtcgttc agctgaacga aatcgatggc
    14881 gaagcgctcg gcgacgcgga ccgctccgtc gatggggagc gtcgcgccgc ggtggtagct
    14941 gtagggtgtg cagcatccgc cgcccatcac ccagcgcgaa cctttgagcg gcggcgcgat
    15001 catgaccgca ccgggtccga cggcgagggg atccgtcacg aaggtcagct cccgaggcgg
    15061 cgccggcgcg gggtcgcgat cgcctttctc ttcttgcttg ggagccgtcg ccacgccgat
    15121 ggcgaaacga tgccgcaagg ccttcgggag ggtcgcgtcc ttcgcaagcg tcacctccat
    15181 gaacacgaag cccgaaccgc cggccgctag cgccgtgccc ttggccccgc cgttcagccg
    15241 cgtcatcttg gcgagacctt ccccctcgag cgtgccgatc gcggccccgc tatgggcatc
    15301 gagcgtctcg atcttttcga ctgtcaccgc gctcggggcc atgttcatga gcacgatctc
    15361 ataggcgaga tggatcttgc cgtcggagcc gagcacggga tttggcggcg cgatcggcgt
    15421 tgccacgagc ggcgtcgcga cgacttcggc ttgctccgtc tcggccttga ccgcactcgc
    15481 caagccgaac gcgaccgtcg cagcggcgat caaggaccac gtgaacattg tcattctgat
    15541 gctgtcgcaa cgcatggaca tcgagttgga acctcctcgg tgatctggtt ttgcgaagga
    15601 attctagcct agtccgagcg ccttgcgcgc ctttgcactg atcgactggc gggttgcgaa
    15661 gaatccgtcc catggatctc ccttgagcgc cttgaggcgc gcgggcagcg ttttcgccgt
    15721 gtagggggcg gcggccttca gcgctttcag ctcatcccag cggagcggac aggagacggg
    15781 cgcgcccttc ttggccctgg tcgagtaatt gacgatggcc gagccgccgc gctgattgcg
    15841 gagatagtcg acgaagatct tgccctcccg cgtcttcttg agcggattcg ccgtgtagcg
    15901 atcgggtcgc gcgctgacga gcgcgtccgc gatcgctttg gtgaaggcct tcacttcatc
    15961 ccagccaagt ttcggcgtga gcggcgccac gagatgcaag cccttcccgc ccgtcgattt
    16021 gaggaagctc ttgaagccga gatcgccgag gaagtcgcgc acttcgaccg cggaagcctt
    16081 caaagtggca aggtcgaggt cctcgtcggg atcgaggtcg aacacgatcc ggtcgggctt
    16141 atcgatgtga ccgagcgaga cgccccaatc gtgaatctcg agcacgccga tctggaccaa
    16201 tgcgaacagg ccggcttcgt catcgatgta gagatatttt ttcttggtct cgccgccggc
    16261 gatcgggact tccttgatgg tgtcgctcat gcctttcatg gcatggcgct ggaagaagca
    16321 ctccttggtg tagccctcgg ggcagcggac caggctgatc ggccgcctca agacataagg
    16381 cagcatgaag gacgcgaccg cctcgtaata gcgggcgagg tcgaacttgg tcaggtcgat
    16441 atccgggaac agcaccctgt cgggattggt gagggtgatg ccgtcgaagg tggcgccgtc
    16501 tcgcccgcgc gcgctcgcct tggccatgct cgtctcctcc gcatcggtct gttcgcgatg
    16561 gacctcgcgc accggcttgt cctcgcgaag cccttgaaag cttggatgcc tgagccgccc
    16621 gtcgcgggtc ttctccgcgt aggtaacctg gcagacgagc ttcggttcga gccacacggc
    16681 gcccttgcgc tccggcgcgg gcacctcgac gaaagcggac gtggcgcgct cgagaggctt
    16741 gaaccttcgc gacaagttcc tgaaatcggc cgcgtcgaat ccggtgccga ccttgccggc
    16801 atagacgagc tcgacgccgg aaaactcgcc gagcagaagc gaggagaacg gcttcccccg
    16861 cacatcggag cggctatagc cgccgatgac gaattcatcg ccgcgcttgc atttgatctt
    16921 gagccagtcc ttgccgcgcc ccgaaaggta gggccggtcc gcccgcttcg acacgatgcc
    16981 ttcgaggccg gccgcgcagg cttgcgcgag aaactcggag cccgagccct cgacgtggtc
    17041 cgcgtaaacg agaaagttcg agacgccctt cgccgccatg aggcccgcga gcttctcgcg
    17101 ccggtcctta agaggcttct tgcgccagtc ttttccgtcg agcgagagca gatcgaagag
    17161 aaaatagcgg atcgagccat gcggactgtc gatatgctct tgcagcgatt tgaaatcggt
    17221 gacgccggag gggagcacga aggcgatttc gccgtcgaga agcacggtct cagcggggaa
    17281 cgtgagaagc gccttggcga tcgcggggaa gcgcaccgtc cagtcgagac cgctccggct
    17341 gaacagcgtg atctcgtcgc cgaccttgcg gccgatgatg cggtagccgt catatttgat
    17401 ctcgtggaac cagcccggtc ctgtggggac ctcgctttcg agcgtggcga gctcgggcgc
    17461 gacgaagtcc ggacctagcg cgcggcgggt cttcgctttg gctttgccgc ggccttgcgc
    17521 ctcacgcgcg ccgccggagt ttttttttga gccttcgagg tccagaccgg gacgtcgcct
    17581 gactggatct ggtccatggt gcggccgctg atcgcgctcg tgtcgtagat ttgcgtgggc
    17641 tcgttgccgt cgccggcata ggcgtcgcgc ttcttgatga ggagccagtt ctggcggccg
    17701 cggtcctcct tccggggctt catgcgcacg agcacgaagt cgcccttgag ccgcttgccg
    17761 ttgagccgga acttgaggtc gcccttggca agcccctcgt cggggtcgcc gagcgggtcc
    17821 caggtgccct cgtcccagat catcaccggg ccaccgccgt actcgccctt ggggatgacc
    17881 ccctcgaagc cgccatattc gagcgggtgg tcctcggtca tcaccgcgag ccgcttctgg
    17941 ctcggatcga ggctcgggcc tctcgtcacg gcccagctct tcagcacccc gtcatgttcg
    18001 agcctgaaat cgaaatgggt gcggcgcgcg gcatgcttct ggatcacgaa gctgttgccg
    18061 ctcgcgcgct tgatcttggc cttgggctcg ggtgtcttgg cgaaatcccg cttcgccttg
    18121 taggtggcga ggagatcgcg gccaccggcg cccccgcgaa tggcacgcgc catggtccta
    18181 agccgatttc cgcttgggcg tgcctctggc agccttcttg ccgttccccg accgggccga
    18241 ggccgtgccg cgcttggccg tctccttggc gccggcgagc ttcccttgct taacgctttt
    18301 cttcagcgcg tccatgatgt tgatcacctt ggcgctgccc ggcatctcgg tcacgatctc
    18361 gggcgccctg tgctccagct tggcctggat cagctcccac accgcctcgt tatacgcatc
    18421 cttgagcgcc tcgggctcga acttgccagt cttgcgcttg atcagctcct tggcgaggtc
    18481 gagcgcctcc ttgtcggcct tgatctcggt gatttccttg aacagcggct ctgccgcacg
    18541 gatttcgttg gcatgccgca gcacctcgag aaggaggcct ttgccgaacg gcttgatcgc
    18601 aacgatattg ccgcggccgc gcaggatgaa ttgaccgacg ccgaccttgc ccgtgtcctt
    18661 caacgccttc tggatgatgg aatagccctc atcggccatg tcgccgtcgg gcaggaggta
    18721 gtatggcgtt tcgtagtagc gcgcgtcgat cacctcgtcg tcgacgaagc gcacgagctc
    18781 gatcgtctgc ttggcctcga gcttgagctc gtcgatctcc tgcgggtcga tcaggacata
    18841 tttgcccttc tcgtattcgt agcccttggt gatgtcctcg ttctcgaccg ggcccacgcc
    18901 gggcaccacc ttctggtagc ggatgcgctt
//
LOCUS       DASUQP010000063         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1117738, whole genome shotgun sequence.
ACCESSION   DASUQP010000063 DASUQP010000000
VERSION     DASUQP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117738"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1238)
                     /locus_tag="VFY74_02970"
     CDS             complement(24..1238)
                     /locus_tag="VFY74_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEX5930703.1"
                     /translation="MSRPALEVADIFRDHGPAWRRANAGHVSLPQLKVMSAIERCRTA
                     ALGGHVARCENGVCGHTVIAFNSCRNRHCPKCQGAAARDWLEEREAELLPVPYFHVVY
                     TLPGPIADIAYQNKAVIYDILLKASAEATLTIAADPRRLGARIGITSVLHTWGSAMTH
                     HPHVHMIVPGGGLSDDGGKWIACRPNFFLPVRVLSRLFRRLVIEMLIAAHDAGKLVFF
                     GDHASLADAGAFAALLAPLRRSEWVVYSKRPFGGPEAVLAYLSRYTHRVAISNRRLIA
                     ANERSVTFKVKDYRVEGPDRFKTMTLATGEFIRRFLIHVLPKGFHRIRHYGLLANGNR
                     ADNVAKARELLAVPTPVAEPEPEAAASDQASLHARACPCCGSRMVIIEVFERGSQPKH
                     WPTPVLLRIDTS"
     gene            complement(1251..2186)
                     /locus_tag="VFY74_02975"
     CDS             complement(1251..2186)
                     /locus_tag="VFY74_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5930704.1"
                     /translation="MTNPTTVSPLRQRMIEDMTARGLGQQTQRGHIRACKRFAAYLKR
                     SPETATAEDVRLFQLHLMESGLTIQNRNRTMTGLRFLLRVTMRRLDLAAEIFHMKEPF
                     RVPQILSAEEAKRLLLMAGKPQVRMLFSIGYGAGLRVSEVVKLKVKHIDSALGVIRVE
                     QAKGKKDRQVMLSPETLDLLREWWKVRSKKYDHGVPLQERWLFPGRRKGLHLTPRQVT
                     RLFHETVEAAGIRKKVTLHALRHSFATHLFDRGVDIRTIQALLGHEKLETTARYTRVA
                     TGLITAVESPLDRLSLPGKAKRKKGKPKPDGETGT"
BASE COUNT          393 a          754 c          781 g          416 t
ORIGIN      
        1 atcgttgcgg tgcggtcgcc atctcatgag gtgtcgatcc tgagcaagac cggcgtcggc
       61 cagtgtttgg gctgcgagcc gcgttcgaat acctcgatga tgaccatgcg gctgccgcaa
      121 cagggacagg cgcgcgcgtg aaggctcgcc tggtcgctgg cggcggcttc cggctcgggc
      181 tcggcgaccg gcgtcggcac agcgagcaat tcgcgcgcct tggcgacgtt gtcggcgcgg
      241 ttgccgttgg cgagcaagcc gtagtggcgg atgcgatgga agcccttggg caggacgtgg
      301 atcaggaacc ggcggatgaa ctcaccggtg gcgagcgtca tggtcttgaa gcgatccggc
      361 ccctcgaccc gatagtcctt gaccttgaag gtgacgcttc tctcgttggc ggcgatcagc
      421 cgacggttgg agatggcgac gcgatgggtg tagcgggaca gataggcgag cacggcctca
      481 ggtccgccga acggcctctt cgaatagacg acccactccg atcgcctgag cggagccagc
      541 agggcggcga acgcgccggc atcggcaaga gaagcatggt cgccgaagaa caccagcttg
      601 ccggcatcgt gtgccgcgat cagcatctcg atgacgaggc gccggaacag gcgggagagg
      661 acacgcaccg gcaggaagaa gttcggccga cacgcaatcc acttcccacc gtcatcggag
      721 agcccgccgc ccggcacgat catgtgcacg tggggatgat gcgtcatcgc cgatccccat
      781 gtgtgcagca ccgaggtgat gccgatcctg gcgccaagcc gcctgggatc ggccgcaatg
      841 gtcagcgtcg cctcggccga cgccttgagc aggatgtcgt agatgacggc cttgttctgg
      901 taggcgatgt cggcgatggg gcccggcaga gtgtagacga cgtggaaata cggcacgggc
      961 agcagctctg cttcccgttc ttcgagccag tcacgcgccg ccgcgccctg gcacttcggg
     1021 cagtggcgat tgcggcagct gttgaaggcg atgaccgtgt ggccgcacac gccgttctcg
     1081 cagcgcgcga cgtgcccgcc gagcgccgcc gtgcggcagc gctcgattgc actcatcacc
     1141 ttcaactggg gcaggctcac gtgccccgca ttggcgcgac gccaagcagg gccgtggtcg
     1201 cggaagatgt ccgcgacctc cagagcagga cgggacacgc gcgacggcgg ctatgtcccg
     1261 gtctcgccgt ccggcttggg cttgcccttc ttgcgcttcg ccttgcccgg aagcgacaac
     1321 cgatcgagcg ggctctcgac ggccgtgatc agacccgttg cgacacgcgt atagcgggct
     1381 gtggtctcca acttctcgtg accgagcaag gcctggatcg tcctgatatc gacgccccga
     1441 tcgaacaggt gggtcgcaaa gctgtgccgc agagcatgca gcgtgacctt cttcctgatc
     1501 ccggccgcct cgacggtctc gtggaataga cgcgtgacct gccttggcgt cagatgcaat
     1561 cccttccggc ggccggggaa cagccagcgc tcctgcaacg gcacaccgtg atcgtacttc
     1621 ttcgagcgca ccttccacca ctcgcgcaac agatcgagcg tctcgggcga gagcatcacc
     1681 tggcgatcct tcttgccctt cgcttgctcg acgcgaatga cgccgagcgc gctgtcgatg
     1741 tgcttgactt tgagcttgac cacctcgctg acacgcaagc cggcgccgta gccgatgctg
     1801 aacagcatgc gcacctgcgg cttgcccgcc atcagcagca gccgcttggc ctcctccgcg
     1861 ctcaggatct gcgggaccct gaacggctcc ttcatgtgaa agatctcggc cgccagatcg
     1921 agccgccgca tcgtcacgcg cagcagaaag cgcaggccgg tcatggtgcg gttgcggttc
     1981 tggatggtaa gtccgctctc catcaggtgg agctggaaca ggcgcacgtc ttcggccgtg
     2041 gccgtctctg gcgatcgctt caggtatgcg gcaaaccgct tgcaggcgcg gatgtggccg
     2101 cgctgcgtct gctggccgag accacgcgcc gtcatgtctt cgatcatgcg ctggcgcagc
     2161 ggggaaacgg tggtagggtt ggtcatgggg cgttcctgtc cctgagtgag gttgccaaac
     2221 acctcgatcc tcaaggacgg gggctcccca tcccgttatg aactccttct ccgccggtta
     2281 ccggcctcgc tgaaaagccg ctcccgcgaa gcggctatcg cggcagcgat ttagtgctag
     2341 ggtc
//
LOCUS       DASUQP010000064         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_28105,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000064 DASUQP010000000
VERSION     DASUQP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28105"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFY74_02980"
     CDS             complement(<1..183)
                     /locus_tag="VFY74_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5930705.1"
                     /translation="MIRIENLVKTFGSKRAVDGVSFNVERGEVLGFLGPNGAGKSTTM
                     RMITGFMPPSAGRVSVG"
     gene            271..1206
                     /locus_tag="VFY74_02985"
     CDS             271..1206
                     /locus_tag="VFY74_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5930706.1"
                     /translation="MRVLLAAVLSISAAVAFAQPSLKMMIPANPGGGWDQTGRNLAAA
                     MQSAKLVSGVQFENKGGAAGTIGLAQFVNSAKGDPNAVMIGGMVMVGGIILNKSAVNL
                     SQVTPIARLTSEWEVIVVPANSPHKNLDSLLKAFKENPGKVSWGGGSAGGTDHILVGL
                     IAKDIGVDPAKVNYVAFKGGGEAIAAILGGHVTAGVSGLGEFAEQIKSGKMRALAQSS
                     PKTLEGIPSLKEQKVNVELGNWRGIFGGPGLTPQQRDNLVKLVQQATETPAWKQTLEK
                     LGWEGWFLGGDAYVKFLKEDEARIAGIMESLGLKK"
     gene            1217..1696
                     /locus_tag="VFY74_02990"
     CDS             1217..1696
                     /locus_tag="VFY74_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5930707.1"
                     /translation="MRSTAEVALSLGVVALGGLVAGVTATLPSEGGYAGIGPNFIPAV
                     VAAGIIVLGLWLCYEAFTGGWRQMGEQTEVFEPSPFIWVSAGLFGHMALIGTSGFVVA
                     GTVLFACVARGFGSRRIVRDVAIGLVLSLGVFLFFVKLLNVNLPAGWLGPVLGGAGI"
     gene            1697..>2103
                     /locus_tag="VFY74_02995"
     CDS             1697..>2103
                     /locus_tag="VFY74_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5930708.1"
                     /translation="MEALSALMTGFAVALKPINLMWGFIGVTLGTFVGVLPGVGPALT
                     VAMLLPLTVKLDPTGALIMFAGIYYGAMFGGSTTTILLNTPGESASIATALEGNRMAK
                     AGRAGPALATSAIGSFVAGTIATLLLTLLAPIVV"
BASE COUNT          344 a          667 c          702 g          390 t
ORIGIN      
        1 gccgacgctg actcgtccgg cgctcggcgg catgaagccc gtgatcatgc gcatggtggt
       61 ggacttgccg gcgccgttcg gacccaggaa gccaagcacc tcgccgcgct cgacgttgaa
      121 ggaaacgccg tccacggcgc gcttggagcc aaatgtcttg accaggtttt caatgcggat
      181 catgcgttta ctgcgggacc atctttcaag ggggcgcatt atcgcttaaa ttcacgcctc
      241 ctagccgacc ctattccccg gaggagccca atgcgtgtcc ttttagcggc cgtcctgtcc
      301 atcagcgccg ccgttgcctt cgcgcagccg agtctcaaaa tgatgattcc cgcgaaccct
      361 ggcggcggct gggaccagac cgggcgcaat ctcgccgcgg cgatgcaaag cgcgaagctc
      421 gtgtccggcg tgcagttcga aaacaagggc ggcgctgccg gaaccatcgg cctcgcgcag
      481 ttcgtgaact cggccaaggg cgatccgaac gcggtaatga tcggcggcat ggtgatggtg
      541 ggcggcatca tcctcaacaa gtcggcggtg aatctctccc aggtgacgcc gatcgcgcgc
      601 ctcacgagcg agtgggaagt gatcgtcgtg cctgcgaact cgccgcacaa gaacctcgac
      661 agcctgctca aggcgttcaa ggaaaatccg ggcaaggtct cctggggcgg cggctcggcg
      721 ggtggcaccg accatatcct ggttgggctc atcgcgaagg acatcggtgt cgacccggcg
      781 aaggtgaact acgtggcctt caagggcggc ggcgaggcga tcgccgcgat cctcggcggc
      841 cacgtcaccg cgggcgtctc gggtctgggc gaattcgccg agcagatcaa gtcgggcaag
      901 atgcgcgcac tcgcgcagtc gagccccaag acgctcgaag gcattccctc gctcaaggag
      961 cagaaggtca atgtcgagct tggcaactgg cgcgggattt tcggcggacc cgggctcacg
     1021 ccgcaacagc gcgacaacct cgtcaagctg gtgcagcagg cgaccgagac gccggcgtgg
     1081 aagcagacgc tcgagaagct cggctgggag ggctggttcc tcggcggcga cgcctatgtc
     1141 aagttcctaa aggaagacga ggcccgcatc gcgggcatca tggaatcgct cggcttgaag
     1201 aagtaatgag gttgtagtgc gctcgaccgc cgaggtcgcg ctctctctgg gcgtggtcgc
     1261 cctcggcggc cttgttgctg gcgtaaccgc tacgttgccc tcggagggcg gctacgccgg
     1321 catcggtccg aacttcatcc cagccgtggt ggcggccggg atcatcgtgc tcgggctttg
     1381 gctctgctac gaagccttca cgggcggctg gcgccagatg ggcgagcaga cggaggtttt
     1441 cgagccgagc ccgtttatct gggtaagcgc gggactcttc ggccatatgg cgctcatcgg
     1501 cacctctggc ttcgtggtcg ccggtaccgt gcttttcgca tgcgtcgcgc gcggcttcgg
     1561 cagccggcgc atcgtgcgcg atgtcgccat cggactggtg ctgtcgctcg gggttttcct
     1621 tttcttcgtc aagctgctta acgtgaatct gcccgccggc tggctcgggc cggtcctggg
     1681 cggggcggga atctaggtgg aagcgctgag cgcgctaatg accggcttcg cggtcgcgct
     1741 gaagccgatc aatctgatgt ggggatttat cggtgtgacg ctcggcacgt tcgtcggcgt
     1801 tctgcccggc gtcggaccgg cgctcaccgt ggcgatgcta cttccgctca cggtcaagct
     1861 cgacccgacc ggcgcgctca tcatgttcgc cggcatctac tacggcgcca tgttcggcgg
     1921 ctcgaccacg acaattctcc tcaacacgcc gggcgaatcg gcatcgatcg ccaccgcgct
     1981 cgagggcaac cgcatggcga aggccggccg cgctggaccg gcgctcgcaa cctcggcgat
     2041 cggctcgttc gtcgccggca ccattgcaac gctgctgctc acgcttcttg cgccgatcgt
     2101 ggt
//
LOCUS       DASUQP010000065         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_846586,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000065 DASUQP010000000
VERSION     DASUQP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846586"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            528..1484
                     /locus_tag="VFY74_03000"
     CDS             528..1484
                     /locus_tag="VFY74_03000"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX5930709.1"
                     /translation="MTIAAPASNATFPHRHLIGIEGLSPAEITALLDLSEEAVKLSRQ
                     IDKKRDDLRGRTLINLFFEASTRTQSSFELAGKRLGADVMNMAVGTSSVQKGETLIDT
                     AVTLNAMRPDLLVVRHHAAGAVELLSQKVDCSVINAGDGSHEHPTQALLDALTIRRHK
                     NRIEGLIVAICGDILHSRVARSNILLLNALGARVRVVAPSTLLAAGIERLGVEVYTSM
                     REGLADADIVMMLRLQRERMTSSLIPSQREYFHFFGLDYEKLACAKPDALVMHPGPMN
                     RGVEIDSNVADDARSVIREQVEMGVAVRMAVLQALARHLPNR"
     gene            1496..2821
                     /locus_tag="VFY74_03005"
     CDS             1496..2821
                     /locus_tag="VFY74_03005"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5930710.1"
                     /translation="MLDQSQRPPEAMALVNARLIDPSSRLDQLGGVLIRDGLIADFGP
                     YIDAGAAGDAEVLDCDGLVLAPGLIDMMVFTGEPGHEHRETLATASRAAAAGGVTTIA
                     CMPNTDPVIDDVALVDFILRRARDTALVHVHPMAAMTKGLLGEEMTEIGLLSEAGAIA
                     FANGKSSLANAKVMRNVLAYAKDFGAVIVHHLEDPWLAKDGVMNEGEVATRLGLRGIP
                     AAAETIMLERDIRLVELTGGRYHAAQISCRASLDVIRAAKLMGLPVTCGVSINHLTLN
                     ENDIGPYRTFFKMRPPLRSEEDRRAMVEGVASGDIDVIVSVHDPRGAEGKRRPFAEAA
                     DGAVGLETLLGAALRLYHNGDVDLPQLLAALTANPARLLRLGSGRVARGAPADLILID
                     LDRPWVVEPERLRSKAKNTPFDGARLQGRAILTIVAGRVVYNYADASGP"
     gene            2845..3456
                     /gene="plsY"
                     /locus_tag="VFY74_03010"
     CDS             2845..3456
                     /gene="plsY"
                     /locus_tag="VFY74_03010"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5930711.1"
                     /translation="MSPAEWSGVFADLSAFAGGYLLGSIPFGLILAKLAGLGDLRQIG
                     SGNIGATNVLRAGSKGLAALTLVLDAAKGAAAVLIAARFGEAAALSAGLGAFLGHLFP
                     VWLGFRGGKGVATYLGVLLGLYWPAAAAFAATWLFVALVTRYSSLAALAASASSILVL
                     ALMGEWPLAALFLLLAVLLYLRHAANIGRLARGEEARIGETKR"
     gene            3437..>3603
                     /locus_tag="VFY74_03015"
     CDS             3437..>3603
                     /locus_tag="VFY74_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020628880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEX5930712.1"
                     /translation="MAKRSDRASAPATPAPRLSRAQRLAWLRLIRSENVGPATFRALV
                     NEFGGAEAAIAA"
BASE COUNT          566 a         1259 c         1106 g          672 t
ORIGIN      
        1 cggcctctcg cgttacggtt caaacccgtt cttcgttcaa gctagcaggg cctgcaccag
       61 cacctgtatg ggccgcagcg ccatttgtag ccgggcgggc accgtcccca cccgtagcag
      121 cctacctgct cgatcgacga gtaattcttg gtgaccccag ggagagtcaa cgttccagat
      181 cgccaagtgg tggcgtcagc cttccacgct atcgctccag caaccaacat cgccgctgac
      241 acacctaggg caatcgcaag cttacgcatc ttttgcctcc tggtttgggt tcccctaccg
      301 gcgagcggta gaatgcgcga ccaggatagc tcaagattcg ccgcgcagac accctccgac
      361 cgggccgatc gttaccaagt gcttccgtgc atcatccgct gcgggacatt tgctgcgggt
      421 gaaatcggcg gcaggttcga tctcccttta aagcgttacc ttggggctta ccgcgttctg
      481 ccgccacgct tgccgcctcc ggcgccaaag cctatacagt ccctttgatg accatagcgg
      541 cgccagcctc gaacgccacc tttcctcacc gccatcttat tggaattgaa gggctttcac
      601 ctgccgagat cacggcgctc ctcgatcttt ccgaggaggc cgtcaagctc agccggcaaa
      661 tcgacaagaa gcgtgacgac cttcgcggcc ggacgctgat caacctcttt ttcgaggcct
      721 cgaccagaac gcagagctcc ttcgagcttg ccggcaagcg ccttggcgcc gacgtgatga
      781 acatggcggt cggcacgtcc tccgtgcaga agggcgaaac gctgatcgac acggccgtga
      841 cgctgaacgc catgcgtccc gacctcctcg tggtgcgcca tcacgcggcc ggcgccgtcg
      901 agctcctgtc gcaaaaggtc gactgctcgg tcatcaatgc cggcgacgga agtcacgagc
      961 accccactca agctctgctc gatgctctga ccattcgccg ccacaagaac cgcatcgagg
     1021 gccttatcgt cgcgatctgc ggcgatattc ttcattcgcg ggtcgcgcgg tccaacattc
     1081 tgctgctcaa cgcgctcggc gcccgggttc gcgtcgtggc gccgtccacg ctgctcgccg
     1141 ccggcatcga gcggctgggc gtcgaggtct atacgtcgat gcgcgagggc ttggccgacg
     1201 ccgacatcgt catgatgctc cggctccagc gcgaacgcat gacgtcgagc ctgatcccga
     1261 gccagcgtga atatttccac ttctttggcc tcgactacga gaagctcgcc tgcgccaagc
     1321 ccgacgcgct cgtcatgcat cccggcccca tgaaccgggg cgtcgagatc gactcgaacg
     1381 tcgccgacga cgcccgaagc gtcatccgcg agcaagtgga gatgggcgtg gccgtgcgca
     1441 tggcggtgct ccaggcgctc gcccgccatc ttcccaaccg gtagggcgat gaacgatgct
     1501 tgaccagagc caacgccccc ccgaagccat ggcgctggtc aatgcgcggc ttatcgaccc
     1561 gtcttcgcgt ctcgatcaac ttggcggcgt tctgatcagg gacggcctca tcgccgattt
     1621 cgggccctat atcgacgcag gtgcggccgg cgatgccgag gtgctcgatt gcgacggcct
     1681 cgttctcgca cccggcctga tcgacatgat ggtctttacc ggcgagccgg gacacgagca
     1741 ccgcgagacg ctggcgaccg cgagccgtgc cgccgccgcg ggcggcgtca ccaccatcgc
     1801 ctgcatgccc aataccgatc ccgtcatcga cgacgtggcg cttgtcgatt tcatcttgcg
     1861 gcgcgcccgc gacacggcgc tcgtccatgt ccaccccatg gctgccatga ccaagggcct
     1921 tcttggcgag gagatgaccg agatcggact cttgagcgag gcgggcgcga ttgcgttcgc
     1981 caacggcaag tcgagcctcg ccaacgccaa ggtcatgcgc aacgtgctcg cctacgccaa
     2041 ggatttcggc gcggtcatcg tgcatcacct cgaagatccg tggcttgcga aggacggggt
     2101 gatgaacgag ggcgaggtcg cgacgaggct cggcctgcgc ggcattccgg ccgccgccga
     2161 gaccatcatg ctcgagcgcg acatccgcct ggtcgagctt acaggcggtc gctatcacgc
     2221 cgcccagatc tcgtgccgcg cctccctcga cgtcattcgc gcggccaagt tgatggggct
     2281 tcctgtcacc tgcggcgtgt ccatcaacca cctcaccctg aacgagaacg acatcggccc
     2341 ttaccgcacc ttcttcaaga tgaggccgcc gctcaggagc gaggaggacc gccgcgccat
     2401 ggtcgagggc gtggcgagcg gcgatatcga tgtcatcgtg tcggtccacg atccgagagg
     2461 tgcggaaggc aagcggcgcc cctttgccga ggccgccgat ggcgccgtcg gccttgaaac
     2521 cctgctcggc gccgcccttc gcctttacca caatggggat gtcgatcttc cgcagctcct
     2581 cgccgcgctg accgcaaacc cagcccgcct gctccggctt gggagcggac gcgtcgcccg
     2641 gggcgcgccg gcggacctca tcctgatcga tctcgacagg ccttgggtgg tggagcccga
     2701 gcgcctgcgt tccaaggcca agaacacgcc gtttgacggc gcgcggctgc aaggccgcgc
     2761 catcctgacg atcgttgccg gacgcgtcgt ctataactac gctgacgctt cagggccgta
     2821 gcggcgccct ctgggacggt gaacatgtct cccgccgagt ggagtggcgt cttcgcagac
     2881 ctttcggcct ttgccggcgg ctatctcctg gggtcgatcc ccttcgggct tatcctggcc
     2941 aagctcgccg gcctcggcga tttgcgccag atcggctccg gcaatatcgg ggcgacgaat
     3001 gtgctgcgcg ccggcagcaa ggggctcgcc gcgctcaccc tcgttctcga tgccgcgaag
     3061 ggggcggctg ccgtgctcat cgcggcgcgc tttggtgaag ccgcggctct gtccgccggc
     3121 ctcggcgcct tcctcggaca tttgtttccg gtctggctcg gcttccgggg cggcaagggc
     3181 gttgccacct atctcggtgt cttgctcggg ctctattggc ctgccgccgc agccttcgct
     3241 gcgacctggc tctttgtcgc tctcgtcacg cgctattcgt cgcttgccgc tcttgcggca
     3301 agtgcctcga gcatcctcgt gcttgcgctg atgggcgagt ggcccctcgc ggcgctcttc
     3361 ctcctgctcg cggttcttct ctatctgcgc cacgccgcga atatcggccg cttggctcga
     3421 ggcgaggagg cgcggattgg cgaaacgaag cgatagggcc tcagcgccgg caacgcccgc
     3481 ccctcggctc agccgtgccc aacgcctcgc ctggctcagg ctgatccgga gcgagaatgt
     3541 ggggcccgcg accttccgcg cgctcgtcaa cgagttcggc ggcgccgaag ccgccatcgc
     3601 cgc
//
LOCUS       DASUQP010000066         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_982960,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000066 DASUQP010000000
VERSION     DASUQP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982960"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFY74_03020"
     CDS             complement(<1..427)
                     /locus_tag="VFY74_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5930713.1"
                     /translation="MLPRQSAVQSYTPYALAALRIVTAYLFIQHGTAKLFGFPYQAMF
                     DDLQLLSLIGLAGVLEVVGGLLLGAGLYTRPTAFVLSGMMAVAYFMAHAPQGNVMAPM
                     LNGGELAALYSFIFLLFAVTGPGALSLDARKGSSSRLRGG"
     gene            558..1340
                     /gene="ygiD"
                     /locus_tag="VFY74_03025"
     CDS             558..1340
                     /gene="ygiD"
                     /locus_tag="VFY74_03025"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX5930714.1"
                     /translation="MAASPMPALFFGHGNPMNAIGKNAWTQAWSAIGQALPRPKAILS
                     ISAHWYLPGSSVTAIAQPRTIHDFGGFPPELYAVQYPAPGSPELAARTAQLLEPTPVG
                     LDRTWGLDHGTWSILMHLYPAADIPVVQLGIDKTKAASWHFETAKKLAPLRDEGVLII
                     GSGNLVHNLHTYSWGETAREPYDWALRFETLARNALAAADFAPLVNYEALGRDAMLSA
                     PTPDHYLPLLYVLAQHRRGEPVSFPVEGFDGGSISMLAVQLG"
     gene            complement(1337..2617)
                     /locus_tag="VFY74_03030"
     CDS             complement(1337..2617)
                     /locus_tag="VFY74_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930715.1"
                     /translation="MLAGETAGDDERERRLSKNDRHINASARRLIEEGRQVFRFDTFG
                     SEAFFGDALGLHKAIAGEKNGGVGPGVSPKTALAVGLKVDADALPEQLKAQIKAGKVN
                     LDDPATTLALLELNAVVGITGRFDSAGRITSMGTQCALCHSTVDDSFAPGIGKRLDGW
                     ANRDLNVGAIVSLAPNLKPFTDLLKVDEATVRQVLASWGPGRYDALLDKDGKAFRPDG
                     QSAATLIPPAFGLAGVNLATWTGFGSVPYWNAYVAVTQMHGSGTFFDLRFANPDQYPV
                     AAQSGSGNTRGTPDRVTAKLAALQFYQLAIPAPKAPKNSFDEAAAVRGKSVFNGRAQC
                     ASCHVPPLFTDPGHNLHSPEEMGIDAFQADRSPTRMYRTAPLAGLWSHQKGGFYHDGR
                     FPTLLAVVEHYDRTFRLGLTSQEKAELVEYLKSL"
     gene            complement(2682..3023)
                     /locus_tag="VFY74_03035"
     CDS             complement(2682..3023)
                     /locus_tag="VFY74_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5930716.1"
                     /translation="MLEGALLLAALATGDPQAGEAIYPRCAACHALAYDRVGPRHCGL
                     IGRPAGSVAGFEYSPAMKRSKLTWTRESLERFLADPIGTVPGTTMTYAGVPDAKERAD
                     LIAYLANAKCK"
BASE COUNT          557 a         1001 c         1025 g          553 t
ORIGIN      
        1 ggccgccgcg aaggcgggaa gagctaccct tgcgcgcatc aagactcagc gcgccggggc
       61 cggtgacggc gaaaagcagg aagatgaagc tgtagagcgc agcgagctcg ccgccgttca
      121 gcatcggcgc catcacgttg ccctgcggcg cgtgcgccat gaagtaagcg acggccatca
      181 tgcccgacag cacgaacgcg gtcggccggg tatacagtcc cgcgccgagc agcaacccac
      241 ctacgacctc cagaacgccc gcaagaccga tgagcgagag aagttgcaaa tcatcgaaca
      301 ttgcctggta cggaaagccg aaaagcttgg cggtgccgtg ctgaatgaag aggtaagcgg
      361 tgacgatgcg cagcgcagct agggcgtacg gcgtgtaact ttgaacagcg ctttgtctcg
      421 gtagcatgaa cgtctcctga gtgggttctg attgacccaa tctaccgaga ccgcggccgc
      481 ttgattagcc ggcggcggcg ggattctttg tgcatgcgat tgcatagtcg ccgcgcatgc
      541 cggaagatgg gactgagatg gccgcgtcac caatgccggc gttgttcttc ggacatggca
      601 atccgatgaa cgcgatcggc aagaacgcct ggacgcaagc atggtcggcg atcggccaag
      661 cgcttccgcg ccccaaagcc attctctcca tctcggcgca ctggtatctg cctggcagct
      721 ctgtcacggc gatcgcacag ccgcgcacca ttcacgactt cggcggattt ccgcccgagc
      781 tctacgccgt gcagtatccg gcgcccggat cgccggagct cgctgcgcgc acggcgcagc
      841 tactcgagcc gacaccggtg ggattggacc gaacctgggg actcgaccac ggcacatggt
      901 cgatcctcat gcatttgtac ccggccgccg atattccggt cgttcagctt ggcatcgaca
      961 agaccaaggc ggcgagctgg cacttcgaga ccgcgaagaa gcttgctccg ctgcgcgacg
     1021 aaggcgtgct catcatcgga agcggcaacc tcgtgcacaa ccttcacacc tactcatggg
     1081 gtgaaactgc gcgcgagccg tacgactggg cgttgcgttt cgagacactg gcgcggaacg
     1141 cgctggcggc ggcggatttc gcgccgctcg tgaactacga ggcgctcggg cgcgacgcga
     1201 tgttgtcggc gccgacgccc gaccattacc tgccgcttct ctacgtactc gcccagcacc
     1261 ggcgaggaga gcccgtcagc ttcccggtcg aaggcttcga cggcggctcg atctccatgc
     1321 ttgccgtaca actcggctag agcgacttca agtactcgac gagctcggct ttttcctggc
     1381 tggtgaggcc gagcctgaag gttcggtcgt agtgctcgac cacggcgagc agcgtcggga
     1441 agcgtccgtc gtgatagaag ccgcccttct ggtgactcca aagccctgca agcggagcgg
     1501 tgcgatacat gcgcgtcggc gagcggtcgg cctggaacgc gtcgatgccc atttcctcgg
     1561 gcgaatgcag gttgtgcccc gggtcggtga agagcggcgg tacgtggcag ctcgcgcact
     1621 gcgccctgcc gttgaagacc gacttgccgc gcaccgccgc cgcctcatcg aagctgtttt
     1681 tcggcgcctt cggcgcgggg atggcgagtt ggtagaactg cagcgcggca agcttggcgg
     1741 tgacgcggtc cggtgtgccg cgcgtgtttc ccgagccgct ctgcgccgcg accgggtact
     1801 gatcgggatt agcgaatcgc agatcgaaga aagtcccgga gccatgcatc tgcgtcactg
     1861 cgacatacgc gttccagtag ggcacggagc cgaagccggt ccatgtcgct aggttcacgc
     1921 cggcgagacc aaacgcgggc ggaatcagcg ttgccgccga ttggccgtcg gggcgaaacg
     1981 ccttgccgtc cttgtccagc aacgcgtcat agcgtcccgg tccccagctc gcgagcacct
     2041 gacggaccgt agcctcgtcg actttgagaa gatccgtgaa cggcttgagg ttgggtgcga
     2101 gcgagacgat cgcgcctacg ttgaggtcgc ggttcgccca gccgtccagg cgcttgccga
     2161 tgccgggagc gaaggagtcg tcgaccgtcg aatggcacaa ggcgcattgc gttcccatcg
     2221 acgtgattcg cccggcgctg tcgaacctgc cggtgattcc gacaaccgca ttaagctcga
     2281 gcagagcaag cgtcgttgcc gggtcatcca ggttaacttt ccctgctttg atctgagctt
     2341 tcagttgttc gggcagtgcg tccgcgtcca ctttcaagcc cactgcgagg gcggtcttcg
     2401 ggctgacgcc tggcccgacg ccgccgttct tctcgccggc aatggccttg tgcaacccca
     2461 gggcatcgcc gaaaaaagcc tccgaaccga aggtatcgaa gcgaaagacc tgcctgccct
     2521 cttcgatcaa gcgacgcgcg gaggcgttga tgtgacggtc attcttggac aggcgtcgct
     2581 cgcgctcgtc gtcgcccgcg gtttcacccg caagcatggc gagcgcgatg gccgaagcgc
     2641 cgatgacgga cgcagccgct gcaagtctca tctgagttcc tttatttgca cttcgcgttc
     2701 gcgagatagg cgatgaggtc ggcgcgctcc ttcgcatccg gcacccccgc ataggtcatg
     2761 gtggtgccag ggacggtacc gatcggatcg gccagaaagc gctccaggct ctctctggtc
     2821 caggtgagct tcgagcgctt catcgccggg gaatactcga acccggccac gctgcccgcg
     2881 gggcggccga tcaagccgca gtggcgcggc ccgacgcggt cgtaggcgag cgcatggcag
     2941 gccgcgcagc gcggatagat cgcctctccc gcctgtgggt cgcccgtcgc gagcgccgcg
     3001 agcagcagcg cgccttccag cattacgaga cgaacgccga aggcacgggg ttctcgccga
     3061 gagcgttgcg caggacggcc cagtgcatcg cttcgtcgcc gaggatgctg gccgccgcct
     3121 tggagagatc gcggtt
//
LOCUS       DASUQP010000067        17130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_165673,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000067 DASUQP010000000
VERSION     DASUQP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165673"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFY74_03040"
     CDS             <1..370
                     /locus_tag="VFY74_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930717.1"
                     /translation="KAAGPESRPPQAPLAALPADSKVAAWVKAKATEVKGFARARPLY
                     HFEFWLEPPQEVKGNLAAVTYEFNTPAVMPQSQMSSEKETGFRVSAGGLVCADKVTVT
                     LKFRDGRSQRVEVDGCKLVS"
     gene            complement(384..1364)
                     /locus_tag="VFY74_03045"
     CDS             complement(384..1364)
                     /locus_tag="VFY74_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HEX5930718.1"
                     /translation="MNKKDRRKPAVFKVEEIEVFVPPSAPVPTEPMPLPAPRLRTAPD
                     LGRGLRWGTILITALGALVSLLASIWLYDYVLALIARDDWIGWAAAGLSALVVLALLM
                     IVLREVAGLARLGRLGKIRHEADSAARQNDKPLAADVAHRLKRLYAGRSDVAWGLTRL
                     AEHEHDIMDASELLRLTERTLVAPLDPEARAIVASSAKRVSVVTAVSPGALIDMLFVG
                     AENLRMLRRLATLYGARPGTLSLLKLARMVVTHIVLTGGIALGDDVIQQVIGHGLTAK
                     LSARLGEGLFNGALTTRIGIAAIDVCRPLPYLESQRPRFREMVAEVAGLR"
     gene            complement(1361..2818)
                     /locus_tag="VFY74_03050"
     CDS             complement(1361..2818)
                     /locus_tag="VFY74_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEX5930719.1"
                     /translation="MALSTYTQAAGDAVRSVGDFASGLISPTVKLGVTGLARSGKTVF
                     ITALVHNLMAGARLPFFDAAAQGRLARAYLEPQPDAFVARFAFEKHLADLTGAPPRWP
                     ESTRRISQLRLTIEYGSTKFWKRQLGRETLHIDIVDYPGEWLLDLPLLSLDYRAWSRS
                     ALEQSAVPDRKKTARLWHASLGLVDPSDPADEAVAERLSDLFKDYLREARADPNALST
                     LPPGRFLMPGDLAGSPALTFAPLDAKGVEQFPRNSLWTMMESRYEAYKTYVVRPFFRD
                     HFARLDRQIVLIDVLAALNGGQAAIADLERAMTEILECFRTGSNSLWSSVFGPRIDRV
                     VLAATKADHLHHQSHDRLEDILARLATRAMDRAAYAGAQVKVLALAAIRATREGEARR
                     GGERLPCIVGYPLPGETIGRRRFDGNEEFAIFPGDLPANPEAALRGWQIEQGEMRFVR
                     FRPPNPVRLPSGGFAPFPNIRLDRAIETLIGDYLA"
     gene            3159..3524
                     /gene="dksA"
                     /locus_tag="VFY74_03055"
     CDS             3159..3524
                     /gene="dksA"
                     /locus_tag="VFY74_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX5930720.1"
                     /translation="MSEHQRAYFRRKLLNWREEILRSTKETLQHLHDDSAQHADIADR
                     ATSETERALELRARDRQRKLIAKIDAAIGRLDDGTYGYCEETGEPISLKRLDARPIAT
                     LSVEAQERHERRERVYRDE"
     gene            complement(3744..4523)
                     /locus_tag="VFY74_03060"
     CDS             complement(3744..4523)
                     /locus_tag="VFY74_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5930721.1"
                     /translation="MMRARRSLQLARVVSAVALSSAGALASAAALPESFVYLRDIDPT
                     ILQDMRYAGSNNFTGAPVTGYEAAECVLVRPAAEALAAVQAGLRAKGLTLKVYDCYRP
                     ARAVAAFVEWAKAPDDPTAKTTYYPNLPKAALFPDYIATRSGHSRGATVDLTLVPMVE
                     IEDRSSPADSAAPLPCTAPQEGPASDGSLDMGTSFDCFDVEANTAAPGLSREEEDNRA
                     MLVDAMQARGFKNYPFEWWHFTLEPEPFPDTIFDFPIAPRP"
     gene            5104..7656
                     /locus_tag="VFY74_03065"
     CDS             5104..7656
                     /locus_tag="VFY74_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5930722.1"
                     /translation="MTDIDAPMRYASPAADRSERDGSVGLVVLLALALAAAAVGLAMV
                     SREMAEPFVLAILAALAVIGVFCLFAGAVGILHFGSRQARNDVTKAFVDNLPQGALIT
                     DAAGRVLYANEAYRDLLGLDPEASPVPAPDRAFAGNPHLAETIFRLARAAQQGRALKE
                     EFRLPPPGEGEEEDAGVAPRWFRIAVQPMPPDPSSGRKGALVVWQVDEITTERARAEA
                     SFAKVQAAIAYLDSAPAGFFTADAAGNIDYLNATLAQWLGLDLSEVAGRPLKLAAIMS
                     QDSAALIAPKAKDQAQGTTRRFDIDLVKADGTRLPVRVLHRLPRGGATGKLAHALVLN
                     RAPGELEEAGAAELRFARLFHAAPIAIATVDGDGFVSATNAAFLRLFAASPEEGRRLA
                     LESLVDIDSRPALRRALEAASARQGLIDPVDIAFAGAKERSGRIYLSPIDETDGHNEA
                     AIAYAIDTTEQRALEMQIAQGQKMQAVGQLAGGIAHDFNNMLTAIIGFSDFLLMNHRP
                     TDPAFQDIINIKQNANRAAGLVRQLLAFSRQQTLRPQVLQLGDVLPELSILLGRLLGE
                     NIELTLDQASDLWPVKADLHQSEQVIINLAVNARDAMPDGGKLTIRTANISESDARTL
                     APRQVPPGEYVLLQVIDTGSGMTEEVREKIFEPFFSTKEVGRGTGLGLSTVYGIVKQT
                     GGYIFADSESGKGTTMSVYLPRYIESGAEVEAEKAERPVEKPKDLTGRGTVLLVEDED
                     AVRSFAARALAQRGYRVLEATTGTEALDLFRSHHGDVDLVVSDVVMPEMDGPTLCEQL
                     RRERPDLKVIFISGYAEDAFRQHLAENEDFMFLQKPFDLKQLAATVKAALES"
     gene            7775..9019
                     /locus_tag="VFY74_03070"
     CDS             7775..9019
                     /locus_tag="VFY74_03070"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX5930723.1"
                     /translation="MPDYEVVPPEETGKITRRRRAKPRMPSLTIERYGGRLIYFWFGM
                     RFGALMKLFHRGRYGFTLNCIPDTLALFLWVPWNSVLYWISEAKYRKRAEALTLDQPP
                     IFVIGHWRTGTTFLHDLFSVDPNLAYPTTYETFFPHHFLLTEGHLDRVMKVLLPKKRP
                     QDDVPVGFDRPQEEEFGMMMLGQRTPYLTHAWPRLGPADSEYLDFKGLSEGDKKKWAD
                     AYMWYYRRLALKHGKPLVMKSPANAARLKLLTKLFPDARYVYLARNPLNVFPSTVKLW
                     RALYSVQGLHNPPELDPWLDDYVLDMFARLTEAYEEDKHLIPKDRLVELRYEDFVKDP
                     IASMRDIYRRLDIPGFDQAEAPMRAFLAERAEHRVSRYELPAALKRKVVERLKPYIDR
                     FGYREAVDAAALRSSSVAAAQR"
     gene            9940..11091
                     /gene="recA"
                     /locus_tag="VFY74_03075"
     CDS             9940..11091
                     /gene="recA"
                     /locus_tag="VFY74_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5930724.1"
                     /translation="MSNVALRLVEGNEMDREKALEAAISQIERGFGKGSIMRLGQNDK
                     AVEVESISTGSLGLDIALGIGGLPRGRVIEIYGPESSGKTTLALHVVAEAQKRGGICG
                     FIDAEHALDTVYARKLGVDLEQLLISQPDTGEQALEITDTLVRSGAIDVLVIDSVAAL
                     TPRAELEGEMGEQLPGSQARLMSQALRKLTGSISKSRCMVIFINQIRMKIGVMFGNPE
                     TTSGGNALKFYASVRLDIRRIGAIKEREEVVGNQTRVKVVKNKVAPPFKQIEFDIMYG
                     EGISKSGELVDLGVKAGIVEKSGAWFSFDSERIGQGRENAKAYLKEHPETADRIERAI
                     RQNAGLIAGQILEGGSDPDAAESAEGATAEDEGAVTPLAGGSARRGRRG"
     gene            11771..14491
                     /gene="alaS"
                     /locus_tag="VFY74_03080"
     CDS             11771..14491
                     /gene="alaS"
                     /locus_tag="VFY74_03080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017272215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX5930725.1"
                     /translation="MPSRTNDIRTAFLDFFAKNDHAVLPSAPLVPRHDPTLMFTNAGM
                     VPFKNVFTGAEKPKAPRAATAQKCVRAGGKHNDLDNVGFTARHHTFFEMLGNFSFGDY
                     FKERAILLAWELLTRDFALDKERLLITVFVDDDEAASLWRKISGFPDTKILRIAGSDN
                     FWSMGDTGPCGPCSEIFYDHGPGLQGGPPGGAEPEGDRFVEIWNLVFMQYEQLAAGKR
                     VDLPHPSIDTGMGIERIAAVLQGTHDNYAIDLFGALIEASVELTGVQASGEHAPSQRI
                     IADHLRASSFLIADGVLPSNEGRGYVLRRIMRRAMRHAHLLGAKDPLLYRLVPALIEQ
                     MGRAYPELERGRSLIEETLKLEETRFRETLGRGLRLLDDATKGFKRGATLTGEVAFKL
                     YDTYGFPYDLTEDALKARGIGVDAEGFAAAMERQRAEARKSWAGSGEAATEGLWFELK
                     EELGATEFLGYETESASGGILALIKDGARVDALKEGERGVVIVNQTPFYAESGGQVGD
                     QGTLSGGKGAQFRIEDTQKKLHALYLHEGAVTAGSLRVGDEVLLEVDHARRTPTRAHH
                     SATHLLHEALRQVLGLHVAQKGSLVEPGRLRFDFSHPKPMTDDEIRKVEDLANAMILQ
                     NAPVETHLMTPDQAIAQGALALFGEKYGEEVRVVSMGVDPKSERADHAYSIELCGGTH
                     VRRTGDIGLLKIVSEGAVASGVRRVEALTGDAARAYLDTQVARVREAAELLKVSPDEM
                     VQRLAAILDERRKLERQLADAKRDLALGGGGDAAAAPAAREIGKVRLLARTVQGVAPK
                     DLRGLVDDGKRQLGSGIVAIVGVSEEGKAALVVGVTPDLTATYDAVELVRVGAEALGG
                     KGGGGRPDLAQAGGPDGARAEAALEAIAARIAGEGGAAPAGAEAHRAESA"
     gene            14494..15738
                     /locus_tag="VFY74_03085"
     CDS             14494..15738
                     /locus_tag="VFY74_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HEX5930726.1"
                     /translation="MANDTIWRSWRRQVHDILEVGGDAHPAGRVVNAFLITLIFANAL
                     AFAAEMVDDVAVRYGTYLDAFNVFSVMVFSLEYVLRMWSAVEIPMLSRMPPWRARLRF
                     ALRPIMIIDLLAVLPWYLQSFVSVDLRSLRVFRLFRLLKLVRYSPALQTLGRVIAEEY
                     RALLGALLVILILLLFASTAIYFLERDAQPDKFGSVPAAAWWALATLTTVGYGDVVPI
                     TPLGKIVGGIVMLLGVGMIALPVAIIATGFSQESARHQFVVTWSMVARVPLFASMDEG
                     EIAEITKLLYTRTYLPGVPIVRTGDAGDAMFLIAGGEAIVEASSTKQVVLKEGDFFGE
                     MALLERRRHAHDVVAKTRCRVYVLDTQSLSRLSARHPEILQRIRGMAAARGATNEAAR
                     NARRGRKVRPPSKSKRGAPQAV"
     gene            complement(15742..16968)
                     /locus_tag="VFY74_03090"
     CDS             complement(15742..16968)
                     /locus_tag="VFY74_03090"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX5930727.1"
                     /translation="MNKIKVKNPVADLDGDEMTRVIWQLIKEKLIFPYLDLPLLHFDL
                     SIQNRDLTEDRVTIEAAHAIQSCGVGVKCATITPDEARVKEFGLKTMWKSPNGTIRNI
                     LGGVIFREPIVCKNVPRLVPGWTKPIVIGRHAFGDVYRATDFRVLGKGTLTITFAGAD
                     GTRIEHVVHEFEGPGVALGMFNLDDSIRDFARASFNYGLYRNMPVYLSTKNTILKAYD
                     GRFKDIFAEVFAAEFKPAFAARGLTYEHRLIDDMVAAALKWSGGYVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLMTPDGKTVLAEAAHGTVTRHFRQHQQGRETSTNATASIFAW
                     TGALKHRAKLDNNAELADFAETLERVTIATIEAGFMTKDLALLVGADQGWLSTGGFLD
                     AIDANLRKEMADRAAA"
BASE COUNT         2961 a         5670 c         5678 g         2821 t
ORIGIN      
        1 gaaagcggca ggtccggaat cgcgtccgcc tcaagctccg ctcgcggcgc ttccggccga
       61 cagcaaagtc gcggcctggg tgaaagccaa ggcgaccgag gtcaaaggct tcgcgcgcgc
      121 ccggccgctc taccatttcg agttttggct cgagcctccg caggaggtga agggaaacct
      181 tgcggccgtg acctacgagt tcaatacgcc tgctgtgatg ccgcagtcgc aaatgtcgag
      241 cgaaaaagag acgggcttcc gcgtgagcgc gggcggcctc gtctgcgccg acaaggtcac
      301 ggtcacgctg aagttcaggg acggccgctc gcagcgcgtc gaagtcgacg gctgcaagct
      361 cgtgagctga aaggcaggtc cgctcagcgc agcccggcga cttccgccac catctcgcgg
      421 aagcggggcc tttggctctc cagataggga agcggccggc acacgtcgat cgcggcgatg
      481 ccgatccgcg tggtgagcgc cccgttgaac agcccttcgc cgaggcgcgc cgacagcttg
      541 gcggtaagcc cgtgtccaat cacctgctgg atcacgtcgt cgccaagcgc gatgccgccg
      601 gtcagcacga tatgggtgac gaccatgcgc gcaagcttga gcaaagagag cgtgcccggc
      661 cgcgcgccat agagagtggc gaggcggcgc agcatgcgca aattctcggc gccgacaaac
      721 agcatgtcga tcagcgcgcc gggactgacc gccgtgacca ccgacacgcg tttggccgaa
      781 gaagcgacga tggcgcgcgc ctccgggtcg agaggcgcaa caagcgtgcg ttccgtgagg
      841 cgcaacagct cgctcgcgtc catgatgtcg tgctcgtgct cggcgaggcg cgtgagcccc
      901 caggccacat ccgaccgccc ggcatagagt cgtttcaacc gatgggcgac gtcggcggcg
      961 agcggcttgt cgttctggcg cgcggcgctg tcggcctcgt gccggatctt gccgagacgt
     1021 ccgagccgcg cgagccccgc cacttctctc aggacgatca tcaacagggc gagcacgacg
     1081 agcgccgaga ggccggcggc ggcccagccg atccaatcgt cgcgcgcgat gagcgcaagc
     1141 acgtaatcgt agagccaaat cgaggcgaga aggctcacga gcgcgccgag cgccgtgatc
     1201 aggatcgtgc cccaacggag cccgcggcca agatcgggtg cggtgcggag ccgcggcgcc
     1261 ggaagcggca tcggttcggt cggaaccggc gcactcggcg ggacgaagac ctcgatctcc
     1321 tccaccttga agacggcggg tttccggcgg tctttcttgt tcatgccaga tagtctccga
     1381 tcagcgtctc gatggcgcgg tcgaggcgga tatttgggaa gggtgcgaag ccgccggaag
     1441 ggaggcgtac gggatttggc ggcctgaagc gcacgaagcg catctcgcct tgctcgatct
     1501 gccagccgcg gagcgcggct tcggggttgg cgggaaggtc gccgggaaag atggcgaact
     1561 cctcgttgcc gtcgaaacgg cgcctgccga tcgtttcgcc gggcagcgga taaccgacga
     1621 tgcagggaag cctctcgccg ccgcggcgcg cctcgccctc gcgggtcgcg cggatggcgg
     1681 cgagcgcgag caccttgact tgcgctcccg catacgccgc ccggtccatc gcgcgggtgg
     1741 cgagccttgc gaggatgtcc tcgagccggt catggctctg atgatggaga tgatccgcct
     1801 tggtggccgc cagcacgacg cggtcgatgc ggggcccgaa cacgctcgac caaaggctgt
     1861 tcgaaccggt cctgaagcat tcgaggatct ccgtcatggc ccgttcgaga tcggcgatgg
     1921 cggcttggcc gccgttcagc gccgcgagca cgtcgatgag cacgatctga cggtcgaggc
     1981 gggcgaaatg gtcgcggaaa aacggtcgca cgacataggt cttataggcc tcgtagcggc
     2041 tctccatcat cgtccacagc gaattgcggg gaaactgctc gacgcccttg gcatcgagcg
     2101 gcgcgaaggt gagggcgggc gatcccgcga gatcgcccgg catgaggaag cggcccggcg
     2161 gcagcgtcga tagcgcgttg ggatcggcgc gggcctcgcg gagatagtcc ttgaacagat
     2221 cggagagccg ttcggccacc gcctcgtcgg cggggtccga cgggtcgacg aggccgagcg
     2281 aagcatgcca cagccgagcc gttttcttgc ggtccggcac ggcgctctgc tcgagcgcgg
     2341 accgcgacca ggcgcgatag tcgagactga gcagcggcag gtcgagcagc cattcgccgg
     2401 ggtagtcgac gatgtcgata tgcaaagtct cgcggccgag ctgccgcttc cagaatttcg
     2461 tcgagccata ttcgatggtc agcctgagct ggctgatacg gcgggtgctt tccggccagc
     2521 gcgggggcgc gccggtcaag tcggcgaggt gcttctcgaa ggcgaagcgc gccacgaacg
     2581 cgtcgggctg gggctccaga taggcgcgcg ccagccgccc ctgcgcggcc gcatcgaaaa
     2641 aaggcagtcg cgccccggcc atcaggttat gcacaagggc ggtgatgaaa accgtcttgc
     2701 ccgagcgcgc gaggcccgtc acgccgagct tcaccgtggg cgaaatcagg ccgcttgcga
     2761 aatccccgac gctgcggacc gcgtcgccgg cggcttgggt gtaggtggac agggccacga
     2821 actctaccgt cctttgagct ttgattcggc gggggcgccc tacgccccgc acgagcacga
     2881 acctcggatt tgggccccgt ctgatggccc caaacatgac gcaattgtca acggctgcct
     2941 ttgtggcatt cacgcctttg tcaagacgac tgtccggctt gacttgtcaa ttcgcatccg
     3001 taaataccgg gcttggatcg cgggtgatgt cgtgcgagcc ggaacaggga gcgggccgga
     3061 cggcccgaag gggaacgaaa gcctttttgg acatgactct catgcgggcc aaaagaaaaa
     3121 tcccggaaga ttatcgccca tctgaggatg aacccttcat gagcgagcac cagcgggcgt
     3181 atttccgccg caagctgctc aattggcgcg aagagattct gcgctcgacc aaggagaccc
     3241 tgcagcacct ccacgacgat tcggcgcagc atgccgacat cgccgaccgg gccacctccg
     3301 agacggagcg cgcgctcgag cttcgcgccc gggaccggca gcgcaagctg atcgccaaga
     3361 tcgacgccgc gatcggccgg ctcgacgacg gcacttacgg ctattgcgaa gaaaccgggg
     3421 agccgatcag cctgaaacgg ctcgatgcgc gcccgatcgc gaccctgagc gtcgaggccc
     3481 aggagcgcca cgaacggcgc gagcgcgtct atcgcgacga ataagcttgg atcctcctcc
     3541 cgttgcgcgc ttttgcgcgc cggcaagctg caaatcctca tcctgaggag ccgccgaagg
     3601 cggcgtctcg aaggatgcgg tctgatcgcc atgcttcgag acgcgctcct tcggagcgct
     3661 cctcaagttg cgacggaacg ctgtcacccc acccggctcg gccttcggtc gagccgccct
     3721 ccccatcgaa ggaagggttg acctcaaggc cgcggcgcga tcggaaagtc gaagatcgtg
     3781 tcgggaaaag gctcgggctc gagcgtaaaa tgccaccact caaacggata gttcttgaag
     3841 ccacgcgcct gcatggcgtc gaccagcatg gcgcgattgt cctcctcctc ccggctgagg
     3901 ccgggcgctg cggtgttggc ctcgacatcg aaacagtcga agctcgtgcc catgtcgagg
     3961 ctgccgtcgg acgccggtcc ctcctgcggc gccgtgcagg ggagcggcgc ggcgctgtcc
     4021 gccggcgacg aacgatcctc gatctcgacc atcgggacga gggtcagatc gacggtcgcg
     4081 ccgcgcgaat gacctgagcg cgtggcaata tagtcgggga acagcgcggc cttggggaga
     4141 ttaggataat aggtcgtctt cgcggtggga tcgtccggcg ccttcgccca ttcgacaaaa
     4201 gccgccactg cgcgcgccgg gcgatagcaa tcatagacct tgagcgtcag gcctttggcc
     4261 cggaggccgg cttgcaccgc cgcaagcgcc tctgcggctg gccgcaccag cacgcattct
     4321 gccgcctcgt agcccgtcac gggtgcgccg gtgaaattgt tgctgccggc gtagcgcatg
     4381 tcctggagga tggtcgggtc gatgtcgcgg agatagacga agctctcggg caacgccgct
     4441 gcggacgcca gggcgccggc agaagacagc gcgaccgctg aaaccacgcg agcaagctgc
     4501 aaggaacgcc gcgcgcgcat catgctggca ctctctccgt ctctaacgcc ctgttcgcag
     4561 ggcaatccgt cattccggcg aaagccggaa tccagggacg aatgcactgc acttggcatt
     4621 tctggatacc ggcttacgcc ggtatgacgc gttgcagccc tcaccgttgt ccgcgcaggc
     4681 caaaaaaact ccccgcgaag ggcggggagt ggagaagttt gggggactga catgactggc
     4741 ctcggccagt cggtgtcgga ccattagcag ggaactgccg gcgtccttac gataacaacg
     4801 gctgaagcta ccggcgtcgc ctcatccgcc gcccctacaa cacacagggc taccgtaagc
     4861 tcggactgtc tgtttacagc aacgattcgt taatcacaag caaaaccgac cgtgatattt
     4921 ttggcacagt caatcgaaaa cgggcccttc cggcggtgaa ttttcggccg gtttccggcc
     4981 aaaggctggg cactggcgcc cgccgccgcg gcacggggcg taccctgcgt gctcctgcgc
     5041 gttgggccgc ggcccctatc acattgtgcc tcaacccgat tcgaatcggc tattttgcgc
     5101 ggcatgactg atatcgacgc gccgatgcgc tacgcctcgc ccgccgcaga ccgtagcgaa
     5161 cgcgacggca gcgtgggctt ggtcgtcctg ctcgcgctgg ccttggccgc ggcggcggtc
     5221 ggtcttgcca tggtgtcgcg cgagatggcc gagcccttcg tgctcgccat ccttgccgcg
     5281 ctcgccgtga tcggcgtgtt ctgcctgttc gcgggcgccg tcggcatcct ccatttcggg
     5341 tcccggcagg cgcgcaacga cgtcaccaag gccttcgtcg acaatctccc gcaaggggcg
     5401 ctgatcaccg acgccgccgg gcgcgtgctc tacgccaacg aggcctatcg cgacttgctc
     5461 ggcctcgatc cggaagcgag cccggtgccc gcgccggatc gcgccttcgc cggcaacccg
     5521 catttggccg agacgatctt tcggctcgcc cgcgcggccc agcaagggcg cgcgctgaaa
     5581 gaggagttcc gcttgccgcc gccgggcgag ggcgaggagg aggacgccgg cgtcgcgccg
     5641 cgctggttcc gcatcgcagt ccagcccatg ccccccgatc cgagttccgg ccgcaagggc
     5701 gcgctcgttg tgtggcaggt cgatgagatc accacggaac gcgcacgggc tgaggcgagc
     5761 ttcgccaagg tgcaggcggc gatcgcgtat ctcgacagcg cgccggccgg tttcttcacc
     5821 gccgacgccg ccggcaatat cgactatctg aacgccacgc tcgcccaatg gctcggcctc
     5881 gacctgtcgg aggtcgcagg ccggccgctc aagctcgcgg cgatcatgtc gcaagacagc
     5941 gcggccttga tcgcgcccaa ggccaaggat caagcgcaag gaacgacgcg ccgcttcgac
     6001 atcgacctcg tcaaggccga cggcacgcgc cttcccgtgc gcgtcctgca tcgtctgccg
     6061 cggggcggtg ctacgggcaa gctcgcgcat gcgctggtcc tcaatcgcgc ccccggagag
     6121 ctcgaggagg cgggcgccgc cgagctcaga ttcgcgcgcc tgttccacgc ggccccgatc
     6181 gccatcgcga ccgtcgacgg cgatggcttc gtcagcgcga ccaacgccgc cttcctgcgc
     6241 ctgtttgccg cctcgccaga ggagggccgc aggctcgcgc tggagagcct ggtcgatatc
     6301 gacagccgtc ccgcgctccg ccgcgcgctc gaggcggcct cggcccggca gggcctgatc
     6361 gatcccgtcg acatcgcatt tgccggcgcc aaggagcgga gcggcaggat ctatctcagt
     6421 ccgatcgacg agaccgacgg ccacaacgag gcggccatcg cctatgccat cgacacgacc
     6481 gagcagcgcg cgctcgagat gcagatcgcg caaggccaga agatgcaggc ggtgggccag
     6541 ctcgcgggcg gcattgccca cgacttcaac aacatgctga cggcgatcat cggcttctcg
     6601 gacttcctgc tgatgaacca ccgccccacc gatcccgcct tccaggacat catcaacatc
     6661 aagcagaacg caaaccgggc cgcggggctg gtgcgccagc tgcttgcgtt ctcgcgccag
     6721 cagaccctgc gcccgcaggt gcttcagctc ggcgacgtgc tgccagagct ttccatcctg
     6781 ctcggccgtc tgctcggcga gaatatcgag ctcacgctcg accaggcgag cgatctctgg
     6841 ccggtgaagg ccgatctgca tcaatccgag caggtgatca tcaacctcgc cgtcaacgcg
     6901 cgggacgcga tgcccgacgg cggcaagctc acgatccgca ccgccaatat cagcgagagc
     6961 gacgcgcgca cgctcgcccc gcggcaggtc ccgccgggcg aatacgtgct gctccaggtg
     7021 atcgacaccg gcagcggcat gaccgaagag gtgcgggaga agatcttcga gccgttcttc
     7081 tcgaccaagg aggtcggccg cggcaccgga cttgggcttt caaccgtcta cggcatcgtc
     7141 aagcagaccg gcggctatat ctttgccgac agcgaatcgg gcaagggcac cacgatgagc
     7201 gtgtacctgc cccgctatat cgagtccggt gcggaggtcg aggccgagaa ggccgagcgt
     7261 cctgtcgaga agccgaagga tctcaccggg cgcggcaccg tgctcctcgt cgaggacgaa
     7321 gatgcggtca gaagcttcgc cgcgcgcgcg ctcgcccagc gcggctatcg cgtgctcgaa
     7381 gccaccaccg gcacggaagc gctcgacctc ttcaggagcc atcacggcga cgtcgatctc
     7441 gtggtcagcg acgtggtgat gcccgagatg gacggcccga cattgtgcga gcagctccgc
     7501 cgcgagcgcc ccgacctcaa ggtcatcttt atttcgggtt atgccgagga cgcgttccgc
     7561 cagcacctcg ccgagaacga agactttatg ttcctgcaga agcccttcga tctgaagcag
     7621 cttgcggcca cggtaaaggc cgcgctcgag agctgacggc gcgagcgagc gctttccggg
     7681 cttccgcgca acaggacttc ccttcgccac agatcgcgtc tacacgcggg gttcgaagcc
     7741 cgatccgtgc cattgcccat gttggagttt tcgcgtgcct gactacgaag tcgtgccgcc
     7801 ggaggaaacc ggcaagatca cgagacgccg ccgcgccaag ccgcggatgc cttcgctcac
     7861 catcgagcgc tatggcggac ggctgatcta tttctggttc ggcatgcggt tcggcgcgct
     7921 gatgaagttg tttcaccgcg gccgctacgg cttcacgctc aactgcatcc ccgacacgct
     7981 cgcgctcttc ctctgggtgc cgtggaactc ggtgctctat tggatctcgg aagcgaagta
     8041 ccgcaagcgc gccgaggcgc tcaccctcga tcagccgccg atcttcgtca tcggccattg
     8101 gcgcaccggc acgaccttcc tgcacgatct cttctcggtc gatcccaatc tcgcttaccc
     8161 cacgacctat gaaaccttct tcccccatca tttccttctc accgagggcc atctcgatag
     8221 ggtgatgaag gtgcttctgc cgaagaagcg cccgcaggac gacgtgccgg tcggcttcga
     8281 ccgcccgcag gaggaagagt tcggcatgat gatgctgggc cagcgcacgc cctatctcac
     8341 ccatgcctgg ccgcggctag gccccgccga cagcgagtat ctcgatttca aggggttaag
     8401 cgagggcgac aagaagaaat gggcagacgc ctacatgtgg tactaccggc ggctcgcgct
     8461 gaaacacggc aagcctctcg tgatgaagtc gccggccaat gccgcgcgcc tcaagctcct
     8521 gaccaagctc tttcccgacg cccgctacgt ctatctcgca cggaaccccc tcaacgtctt
     8581 tccctccacg gtcaagctct ggcgcgcact ctattcggtg caaggcctgc acaatccgcc
     8641 agagctcgat ccctggctcg acgattacgt gctcgacatg tttgcgcgac tgaccgaagc
     8701 ctacgaggag gacaagcatc tgatcccgaa ggatcgcctg gtcgagctgc gctacgagga
     8761 tttcgtcaag gacccgatcg cctcgatgcg cgacatctac cggcgcctcg acattcccgg
     8821 attcgaccag gccgaagcgc cgatgcgcgc cttcctcgcc gagcgtgcgg agcacagagt
     8881 gtcgcgctat gaactgccgg cggccctcaa gcgcaaagtg gtggagcggc tcaagcccta
     8941 tatcgaccgc ttcggctacc gcgaggccgt cgatgcggcc gccttgcgct caagcagcgt
     9001 agcggcagcg caaagataaa ggcggcggcg agccctccac caaagcgccc gcgcgcgccg
     9061 aaccgtccta agtttcgtat cgccttacga atgattttca atcccccctt gaggggggag
     9121 cacacgcgcc atgctcgtac gctaccgccc tgccttcgcc gaagcgaagc cgttcctcca
     9181 tgcagttcaa tgcagcaggc gcttcggctt cgcgcaggca ggtccgctac tcgagcgcgg
     9241 gttggcgcgt ggagggggtg agaagcgaac ggcttcgcga cggagaggag tctttgcctt
     9301 cgacccccct ccctgaccct cccccaccgg gcgaggggcg cagtctcgat gtcttccttc
     9361 ttcgcgtgaa gtcgcggcga agttgcgcta tgctgcgtga cgcatcgaac cgaatggggg
     9421 cagaagaaca aggggcgatg cgctcacgac acattaggag ggactcatgc gcaaagttgc
     9481 gttcgctttt gcggcggcgg cgctgatgct gctcgccggg gctctcagtg ccgaggcaac
     9541 cacgggggcc gggacctttg gcctccgcgc cgcggccaag gatttctcgc ccgtcgaaac
     9601 ggcaggctgc cgaacggcgg gccctgtctg caggttcgga cggacatggg tatgccggcc
     9661 gctccggggc tgctggtgtg cccgctgctg gcgctagggc cgaccgcggc agacttgaat
     9721 tgggaggccg cccccgtggc ggcctcttcg cttttgcacc ttcccacgcg cgaaaagaac
     9781 atattgcgaa cggagaacaa aacgtgtaca ttcgctcaag ctcgtccctc ggccccgacc
     9841 caagccgacg caaaggctga atggaactcc cgggcgaggg acgagcggag ccccgatttg
     9901 cgactcagag cttgcttgtg aaataacgga gatcatccca tgtcgaatgt cgcgctgcgc
     9961 ctcgtcgaag gaaatgagat ggatcgggaa aaggctctcg aagcggcgat ctcccagatc
    10021 gagcgcggct tcggcaaggg ctccatcatg cggctcgggc agaacgacaa ggcggtcgag
    10081 gtcgagtcga tctccaccgg ctctctcggc ctcgacattg ccctcggcat cggcgggctg
    10141 ccgcgcggcc gcgtcatcga gatctacggg cccgaatcct cgggcaagac cacgctcgcg
    10201 ctccatgtcg tcgccgaggc gcagaagcgg ggcggcatct gcggcttcat cgatgccgag
    10261 cacgcgctcg acacggtcta tgcgcgcaag ctcggcgtcg atctcgagca gctcctgatc
    10321 tcgcagcccg acactggcga gcaggcgctc gagattaccg acacgctggt gcgctcgggc
    10381 gccatcgacg tgctggtgat cgattcggta gcggccttga cgccgagagc cgagctcgaa
    10441 ggcgagatgg gcgagcagct tccgggctcg caggcgcggc tgatgagcca ggccttgcgc
    10501 aagctgaccg gctcgatttc gaagtcgcgc tgcatggtga tcttcatcaa ccagatccgc
    10561 atgaagatcg gggtgatgtt cggcaatccc gagacgacga gcggcggcaa cgccctgaaa
    10621 ttctacgcct cggtgcgtct cgacattcgc cgcatcggcg ccatcaagga gcgcgaggag
    10681 gtggtcggca atcagacccg cgtcaaggtg gtgaagaaca aggtggcccc gcccttcaag
    10741 cagatcgagt tcgacatcat gtatggcgag ggcatttcga agtcgggcga gctcgtcgat
    10801 ctcggggtca aggccgggat cgtggagaag tccggcgcct ggttctcctt cgacagcgag
    10861 cggatcgggc aggggcgcga gaacgccaag gcctatttga aggagcatcc cgagacggcc
    10921 gacaggatcg agcgcgcgat ccggcagaat gcgggcctga tcgcgggcca gatcctcgaa
    10981 ggcggatcgg acccggacgc cgccgaaagc gccgagggtg caacggccga agacgagggc
    11041 gcggtcacgc cgcttgccgg cgggagcgcg cggcgcggcc gccgcggcta accaaacctc
    11101 tcgctctccc ccctcacccg ccccggcgtt cgccggggca ccctctcccc cgaggggagg
    11161 ggggaaaata ggcgctgtcc gaattcgtca tcctgaggag gccgcgaagc ggccctcgaa
    11221 ggacgcaccg tgagatcgtc atccttcgag gctcgctccg ctcgcacctc aggaagaggt
    11281 aatgaccctg caatatcttc cactcgcccc ttgaggggaa tgcgctaccg accggctact
    11341 tgagcgcggg ttggcgcgtg gaggggggtc agcggcgacg ccgcgttaca ttccgccttc
    11401 cccctacccg gctcgccgcg tgccgcggct cgccaccctc tccccgaggg gagagggaaa
    11461 cgaagcgtca ttcagaggtc ggctggacag ccccctttgc tcaccttatg tgtgacgcgg
    11521 gacgtcaagt cttctccttc tcgggagagg gcacggcggc gcgcgattgt ggcgaatcgg
    11581 ctagggtcgc ctggaagcgc ctttcacgtc ctcatgcgaa cccaaggtcc cccgtcaacg
    11641 cgccatgacc acagccaaac gcaaccgtgc cagacagacg cgcgcgaagg ctgccggccg
    11701 aggccccgcg acccaaaaaa gcgggagcgc caagaaagac agcgcgcccg tggcgcacca
    11761 cgacggctcc gtgccgagcc gcaccaacga catccgcacg gcgtttctcg atttcttcgc
    11821 caagaacgat cacgccgtgc tgccgtcggc gccgctcgtg ccgcgccacg atccgacgct
    11881 gatgttcacc aatgccggca tggtgccgtt caagaacgtg ttcaccggcg ccgagaagcc
    11941 gaaggcgccg cgggcggcga cggctcagaa atgcgtgcgc gccggcggca agcacaacga
    12001 cctcgacaat gtcggcttca ccgcgcgcca ccataccttc ttcgagatgc tcggcaattt
    12061 ctccttcggc gactatttca aggagcgcgc gatccttctc gcctgggagc ttctgacgcg
    12121 cgatttcgcc ctcgacaagg agcgcctgct catcaccgtg ttcgtcgacg atgacgaggc
    12181 ggcttcgctt tggcgcaaga tatcggggtt ccccgacacg aagatcctcc gcatcgccgg
    12241 aagcgacaat ttctggtcga tgggcgatac cggcccgtgc gggccgtgct cggagatctt
    12301 ctacgaccat ggaccgggcc ttcagggcgg cccgcccggc ggcgcggagc cggaaggcga
    12361 ccgcttcgtc gagatctgga atctcgtgtt tatgcagtac gagcagctcg ccgccggcaa
    12421 gcgcgtcgac ctgccgcatc cctcgatcga caccggcatg ggcatcgagc gcatcgccgc
    12481 ggtgcttcag ggcacgcacg acaattacgc catcgatctc ttcggcgccc tgatcgaggc
    12541 ctcggtcgag ttgaccgggg ttcaggcttc cggcgagcat gcgccgagcc agcgcatcat
    12601 cgccgatcat ttgcgcgcct cgtccttcct gatcgccgac ggcgtgctcc cgtcaaacga
    12661 gggccgcggc tatgtgcttc gccgcatcat gcgccgcgcc atgcgccacg cccatcttct
    12721 cggcgccaag gatcctctcc tctatcgcct cgtgccggcg ctgatcgagc agatgggccg
    12781 ggcctatccc gagctcgagc gcggccggag cctcatcgag gagacgctga agctcgagga
    12841 gacgcgcttc cgcgagacgc tcgggcgcgg gctgcggctg ctcgacgacg cgaccaaggg
    12901 gttcaagcgc ggcgccacgc tcaccggcga ggtcgccttc aagctctacg acacttacgg
    12961 ctttccctac gacctcaccg aggatgctct caaagcccgc gggatcggcg tcgatgccga
    13021 gggcttcgcc gccgccatgg agcgccagcg cgccgaggcg cggaagtcct gggcgggctc
    13081 gggcgaggcc gcgaccgagg gcctctggtt cgagctgaag gaggagctcg gcgccacgga
    13141 attcctcggc tacgagacgg agtcggcgtc gggcgggatc ctcgcgctga tcaaggacgg
    13201 ggcgcgcgtc gatgctctga aggaaggcga gcgcggcgtc gtcatcgtca accagacgcc
    13261 gttctatgcc gagtcgggcg gccaggtcgg cgatcagggc accctgtccg gcggcaaagg
    13321 cgctcagttc cgcatcgagg acacgcagaa gaagctgcac gcgctctatc tgcatgaggg
    13381 cgcggtgacg gcgggcagct tgcgggtcgg cgatgaggtg ctgcttgagg tcgatcacgc
    13441 ccggcgcacg ccgacgcgcg cccatcactc ggcgacccat cttctgcatg aggcgctccg
    13501 ccaggtgctc gggctccacg tggcgcagaa gggctccctg gtcgagcccg ggcgcctccg
    13561 cttcgacttc tcccatccaa agcccatgac cgatgacgag atccgcaaag tggaggatct
    13621 cgccaacgcc atgattctgc agaacgcgcc cgtcgagacc catctgatga cgccggacca
    13681 ggccatcgcg caaggcgctc tcgctctgtt cggcgagaaa tacggcgagg aggtccgcgt
    13741 ggtcagcatg ggcgtcgacc cgaagagcga gcgggcggat cacgcctact cgatcgagct
    13801 ctgcggcggg acgcatgtgc gccgcacggg cgatatcggc ctcctcaaga tcgtgagcga
    13861 gggggcggtc gcttccggcg tgcggcgggt ggaggcgctg acgggcgacg ccgcgcgcgc
    13921 ctatctcgat acgcaagttg cgcgcgtgcg cgaggcggcg gagctgctca aggtttcgcc
    13981 cgacgagatg gtccagcgtc ttgccgcgat cctcgacgag cgccgcaagc tcgagcgcca
    14041 gctcgccgac gccaagcgcg acctcgctct tggcgggggc ggtgacgccg cagcggcgcc
    14101 ggctgcgcgg gaaatcggca aggtgaggct gctcgcgcga acggtgcagg gtgtcgcgcc
    14161 gaaggacctg cgcggcttgg tcgatgacgg caagcggcaa ttggggtccg gcatcgtcgc
    14221 catcgtcggc gtgagcgagg aagggaaggc cgcgctcgtc gtcggcgtga cacccgatct
    14281 caccgcgacc tatgacgccg tcgagctcgt gagggtcggc gccgaggcgc tgggcggcaa
    14341 aggcgggggc gggcgccccg acctggcgca ggccggcggt cccgacggcg cccgcgcaga
    14401 agcggcgctc gaggcgatcg cggcgcggat cgcgggcgaa ggcggtgcgg cgcccgccgg
    14461 cgcggaggct catcgcgcgg aaagcgccta ggaatggcca acgatacgat ctggcgaagc
    14521 tggcggcggc aagtccacga cattctcgaa gttggcggag acgcgcatcc cgcggggcgc
    14581 gtggtcaacg ccttcctcat caccctcatc ttcgcgaacg ccctcgcctt cgccgccgag
    14641 atggtggacg acgtcgccgt ccgctacggc acctatctcg acgcgttcaa cgtcttctcg
    14701 gtgatggtgt tcagcctcga atatgtgctg cgcatgtgga gcgcggtcga aattccgatg
    14761 ttgagccgca tgcccccgtg gcgggcgcgg ctccgcttcg cgctcaggcc gattatgatc
    14821 atcgacctgc tcgcggtgct gccctggtac cttcagtcct ttgtctccgt cgatctcaga
    14881 agcctcaggg tgttccgcct gttccggctg ctcaagctcg tgcgctattc gcccgcgctg
    14941 cagacgctcg gccgcgtcat cgccgaggag tatcgcgccc tgctcggcgc gctcctcgtc
    15001 attttgattt tgctcctgtt tgcctcgacc gcgatctatt tcctcgaacg cgacgcgcag
    15061 cccgacaagt tcggctcggt gccggcggcg gcgtggtggg cgctcgccac cttgaccacg
    15121 gtcggctatg gcgacgtcgt tccgatcacg ccgctcggca agatcgtggg cggcatcgtc
    15181 atgctgctcg gcgtcggcat gatcgctctc ccggtcgcca tcatcgccac cggattcagt
    15241 caggagtcgg cccgccacca gttcgtggtg acctggagca tggtggctcg cgtgccgctc
    15301 tttgcctcca tggatgaggg cgagatcgcc gagatcacca agctgctcta cacgcggacg
    15361 tacttgccgg gcgttcccat cgtccggacc ggcgacgcgg gcgacgccat gtttctcatc
    15421 gccggcgggg aggcgatcgt cgaagcaagc tcgacgaaac aggtggtgtt gaaggagggc
    15481 gatttcttcg gggagatggc gctgcttgag cggcgccgtc acgcgcacga tgtggtcgcc
    15541 aagacgcggt gccgcgtata cgtgctcgat acgcagtcgc tgtcgcgcct gtcggcgcgg
    15601 catcccgaga tcttgcagcg gatccgcggc atggcggcgg cccgcggcgc gacgaacgaa
    15661 gccgcgcgca acgcgcggcg cggacgcaaa gtccggcctc cgtcgaagag caagcgtggc
    15721 gcgccgcaag cggtctgagt ctcaagccgc cgcgcggtcg gccatctctt tgcgaaggtt
    15781 ggcgtcgatc gcgtcgagga agccgcctgt cgacagccag ccctggtcgg cgccgacgag
    15841 aagcgcgagg tccttggtca tgaagcccgc ttcgatggtc gcgatcgtga cccgctccag
    15901 cgtctcggcg aagtcggcaa gctcggcgtt gttgtcgagc ttggcgcggt gcttcagcgc
    15961 gccggtccag gcgaagatcg aggcggtggc gttggtcgat gtctcgcggc cctgctgatg
    16021 ctggcggaaa tgtcgtgtca cggtgccgtg cgcggcctcc gccagcacgg tcttgccgtc
    16081 cggcgtcatc aacacgctgg tcatgagccc gagcgagccg aagccctgcg ccaccgtgtc
    16141 ggactggacg tcgccatcgt agttcttgca cgcccacaca tagccgccgg accatttcag
    16201 cgccgccgcg accatgtcgt cgatcagccg gtgctcgtag gtcaggccgc gcgccgcgaa
    16261 cgcaggcttg aactccgccg cgaacacctc ggcgaaaatg tccttgaagc ggccgtcata
    16321 ggccttgagg atcgtgttct tggtcgagag atagaccggc atgttgcgat agagcccgta
    16381 gttgaacgag gcccgcgcga agtcgcgaat cgaatcgtcg aggttgaaca tgccgagcgc
    16441 aacgccgggc ccctcgaatt catgcaccac atgctcgatg cgggtgccgt ccgcgccggc
    16501 aaacgtgatg gtgagcgtgc ctttgccgag cacgcggaaa tcggtggcgc ggtagacatc
    16561 gccgaaagcg tgacggccga tgacgatcgg cttggtccat cccggcacga gccgcggcac
    16621 gttcttgcag acgatgggct cgcggaagat gacgccgccg agaatattgc ggatcgtgcc
    16681 gttcggcgac ttccacatcg tcttgaggcc gaactccttg acgcgcgctt cgtcgggcgt
    16741 gatcgtggcg catttgacgc cgaccccgca ggattgaatc gcgtgcgcgg cctcgatggt
    16801 gacgcggtcc tcggtcagat cgcgattttg gatgctgagg tcgaaatgga gcagcggcag
    16861 gtcgagataa gggaagatca gcttctcctt gatcagctgc cagatgaccc gcgtcatctc
    16921 gtccccgtcg aggtcggcga ccgggttctt caccttgatc ttgttcacgt gcgcaaatct
    16981 ccggtgcgcc ccattgcggg gaacccgctc tctatagcat cgtgcaacag cgatgaaagc
    17041 aagaaccgag agcggaatgc gggcgcgcgc cgcgggggcg ggcgccgccg cgctggtcga
    17101 ccaggccacc gtgtacgaca gcgttgccga
//
LOCUS       DASUQP010000068        16131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1255739, whole genome shotgun sequence.
ACCESSION   DASUQP010000068 DASUQP010000000
VERSION     DASUQP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 16131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16131
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255739"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..566
                     /locus_tag="VFY74_03095"
     CDS             141..566
                     /locus_tag="VFY74_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5930728.1"
                     /translation="MRTKTAIVAIALAGALAGAGLTFARDAGDQNAVTAEKLIAEALA
                     GDPSKEVTAQLYTFPPGTVLSWHIHPDAHEIAYVIEGTLTFQRAGEAPEQLAAGAADY
                     LAPNIVHRGMNTTDKPVRLFVVRIKPKDKPLVEEVSPPD"
     gene            complement(580..714)
                     /locus_tag="VFY74_03100"
     CDS             complement(580..714)
                     /locus_tag="VFY74_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930729.1"
                     /translation="MFGPFTPYDLGMRLNDEVTLFHWLIALPAWAIGLYTLWRKNSRR
                     "
     gene            complement(1279..1707)
                     /locus_tag="VFY74_03105"
     CDS             complement(1279..1707)
                     /locus_tag="VFY74_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930730.1"
                     /translation="MTEPPASDTPAETTWPPVVWAARLSVYLLAQGALVLLAYAYYGF
                     DSHPDSFALGFRIDPYLAAVQLLWGAAGTYIGFFRPRYALGFVLAFAAFFTALAALGS
                     FTSYDLGMRLNDEVTLFHWLIALPAWAIGLYTLWRRSGRR"
     gene            1856..2824
                     /gene="mdh"
                     /locus_tag="VFY74_03110"
     CDS             1856..2824
                     /gene="mdh"
                     /locus_tag="VFY74_03110"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5930731.1"
                     /translation="MARSRIALIGAGQIGGTLAHLAGIRELGEIVLFDVVDGVPQGKG
                     LDLSQSAPVEGYVPKFKGTNEYAELKDADVVIVTAGVPRKPGMSRDDLLEINLKVMEQ
                     VGAGLRKYAPEAFVICVTNPLDAMVWALQKTSGLPRNKVVGMAGVLDAARFRFFLAEE
                     FDVSPEDIHTMVMGGHGDTMVPLIRYSSVAGIPLADLVKMRWITQKRLDDIVQRTRDG
                     GAEIVGLLKTGSAFYAPAAAALEMAESYLKNQKRVLPCAAYLNGEYGVKGIYAGVPII
                     IGAKGVERVIELDLTSAEKDAFMHSVGSVRALVELCQRIAPSLATA"
     gene            2883..4046
                     /gene="sucC"
                     /locus_tag="VFY74_03115"
     CDS             2883..4046
                     /gene="sucC"
                     /locus_tag="VFY74_03115"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5930732.1"
                     /translation="MNIHEHQAKTLLKAYGAPVADGRVAASAQEAADHARALGGDIFV
                     VKAQIHAGGRGKGGGIKLVKTLSELHAEADRMLGMTLVTPQTGPKGRLVRHVYVVKDT
                     VIARELYLSLLVDRGSGRVAFVASTEGGVDIEAVAANTPEKIATLTVDPASGISDFHG
                     RTIASALGLEGSLVKQCGSLVKSLYRLFVEKDASLIEINPLVVTKEGNLVCLDAKVAF
                     DDNALFRHDDIAALRDIHEEDPAEVEAANLGLSYVKLDGSIGCMVNGAGLAMATMDII
                     KHYGGEPANFLDVGGGADQERVTEAFTIILSDPHVEAVLVNIFGGIMRCDTIAEGIIA
                     AARETHIAVPLIVRLEGTNVERGKELLSQSGLAITSADDLDDAAKKAVAAVKS"
     gene            4094..4390
                     /locus_tag="VFY74_03120"
     CDS             4094..4390
                     /locus_tag="VFY74_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930733.1"
                     /translation="MAIDEEVLRAARRYAANRDTTVNALVRDYLTSLVAFEEKAAKAR
                     ERLVELSETSEGRMGSWKWKRGDAYEGRLFPRHERPDLRGFGEGATGEEEGNGR"
     gene            4293..4712
                     /locus_tag="VFY74_03125"
     CDS             4293..4712
                     /locus_tag="VFY74_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5930734.1"
                     /translation="MLTRVDCFLDTNVLIYAALGRGQQEKKRATAADLIRKTKFGLSA
                     QALQEFYTVVIRKPDRPLTPVKAMEWIEAFEQQPCVSTDLSLVKTAIEISQRFRLDYW
                     DSAILAAAERLGAEIVYTEDLNHGQSYGSVRVINPFL"
     gene            4730..5608
                     /gene="sucD"
                     /locus_tag="VFY74_03130"
     CDS             4730..5608
                     /gene="sucD"
                     /locus_tag="VFY74_03130"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5930735.1"
                     /translation="MSVLVDKNTRVICQGFTGAQGTFHSEQAIAYGTRMVGGVTPGKG
                     GSTHLGLPVFDTVKDAVEETGADASVVYVPAPFAADAILEAIDAAIPLVVCITEGVPV
                     LDMVKVKRALCGSTTRLIGPNCPGIITPGQCKIGIMPGHIHTPGKVGIVSRSGTLTYE
                     AVAQTTAVRLGQSTCIGIGGDPVNGTNFVDALALFLADDETEAIIMIGEIGGSAEEDG
                     AEFLVEAGNTKPVVGFVAGATAPPGRRMGHAGAVISGGKGDAKHKVEAMRDAGIVVAN
                     SPAALGTTLARLLHDD"
     gene            5680..8541
                     /locus_tag="VFY74_03135"
     CDS             5680..8541
                     /locus_tag="VFY74_03135"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5930736.1"
                     /translation="MTKHELNEVFASTSFLQGANATYLAELHARYEADPASLDPDWRA
                     FFASLEDNKDSVLAEARGPSWGPAPAAVALPARPLGPPGEREALAAARDTLAARMLIR
                     NYRTRGHLAAKLDPLELVERREHPELRPKTYGFAKSDYDRPIYIGGVFGLEFATLREI
                     LDILRRTYSGHIGVEYMHISDPEQRSWIQERIEGREAGFTTKGKTAILKKLIEAETFE
                     HFLDVKYTGTKRFGLDGGESMVPALEQIIKRGGQLGVNEIVIGMPHRGRLNVLANVMA
                     KPYRAIFNEFRGGSAHPDDVEGSGDVKYHLGASSDREFDLNRVHLSLTANPSHLEIVD
                     PVVLGKVRAKQDQREDRERKEVLPLLLHGDAAFAGQGVVAECLGLSGLRGHRTGGSLH
                     FIVNNQIGFTTAPRFSRSSPYPSDVAKMIDAPIFHVNGDDPEAVVYVAKVATEFRQRF
                     GKPVVIDMFCYRRHGHNEADEPAFTQPLMYARIAEHPSVVEVYSRRLIDEGLLTREEV
                     DAMQAEFRAFLEEEFAAGDSYRPNRADWLDGRWSGIGHAEEGARRGDTGVDLERLREV
                     GRRITSFPKSFTTHKTIARIMAARRKMIDEGVGIDWATAETLAFGTLLVEGYRVRLSG
                     QDSERGTFSQRHAVLMDQESEKKYTPLKHVAKDQARFEVVNSMLSEEAVLGFEYGYSL
                     AEPNALVLWEAQFGDFANGAQVIIDQFISSGERKWLRMSGLGLLLPHGYEGQGPEHSS
                     ARLERYLQLCAEDNWQIANCTTPANYFHILRRQLHRQFRKPLVLMTPKSLLRHKRVVS
                     TLAQFGPGSSFHRLLWDDAQFLSDQAIKLVPDDAIRRVVISSGKVYYDLYEEREKRGL
                     DDVYLLRIEQLYPFPARALIHELGRFRDAEMVWCQEEPKNMGAWSFIEPNLEWVLEHI
                     GARHRRPRYAGRPSSAATATGLMNKHFHELKAFVEDALG"
     gene            8559..9863
                     /gene="odhB"
                     /locus_tag="VFY74_03140"
     CDS             8559..9863
                     /gene="odhB"
                     /locus_tag="VFY74_03140"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5930737.1"
                     /translation="MATEIRVPSLGESVVEATVGQWFKQAGDTVVADEPLVELETDKV
                     TIEVPAPVSGVLSEILVKSGATVAVGALLGAIGDGAGAIKPAKAKRPPASSDLAMAEA
                     SAVADEPSVQPPKAPPTEPKKLEREPTPRQEVGVEERPPSPAARKLMIESDLGGDQVI
                     GSGRHGQVLKVDVAKAVEALLKHEPKEKVPPPPRAPVASDDAAREERVRMTRLRQTIA
                     KRLKEAQDTAAMLTTFNDVDMGAVMQLRHDYRDAFEKRHGVKLGFMGFFVKAAVQALK
                     DIPAVNAEIDGEDIVYKNYYHIGIAVGTERGLVVPVLRDADRMSLAEIETAIADFGAR
                     ARSGKLTLEELQGGTFTISNGGIYGSLLSTPILNAPQSGILGMHRIDERAVVRDGAIV
                     ARPMMYLALSYDHRLVDGKDAVTFLVHVKDALEDPKRLVLDL"
     gene            9976..11376
                     /gene="lpdA"
                     /locus_tag="VFY74_03145"
     CDS             9976..11376
                     /gene="lpdA"
                     /locus_tag="VFY74_03145"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5930738.1"
                     /translation="MPQYDLIVIGTGPGGYVCAIRASQLGMKVAVVEKRATHGGTCLN
                     VGCIPSKALLHASERYAEAEDGFAAMGIRVKPALDLAAMLAFKDEGVKGNVDGVAYLL
                     RKHKIDHFHGTGRVLGSGKVEVTFINGEKQPVEAKSIVIATGSEAMDLPGIAIDEKRI
                     LSSTGALSLPEVPKRMAVIGAGYIGLELGSVWSRLGSEVTVIEMLDRIAPGLDGEVAK
                     QLQRTLAKQGLVFKLGTKVVSADSNGGDLKLALEPAKGGKTETLECDVVLVAVGRVPN
                     TEGIGLAEAGLTTDNRGRIVVDENFATNVPGVYAIGDVIRGPMLAHKAEEEGVALAEI
                     LSGQAGHVDYGTIPSVIYTAPEVASVGKTEEELKEAGVAYRKGKFPFTANGRAKVNRT
                     TEGFVKILAEEVSDRVLGVHIIGADAGTMIAEAAVLMAFGGSAEDLARICHAHPTLNE
                     AIKEAALAVDERPIHM"
     gene            complement(11532..12014)
                     /locus_tag="VFY74_03150"
     CDS             complement(11532..12014)
                     /locus_tag="VFY74_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEX5930739.1"
                     /translation="MLLKGTRFVAIMLAALTLGLAFCHLMQLPSRMGWDQYLWVGSTV
                     QGGLYNLFGSVGAVIFVATIIALALLAYFTREHGRPGFGLALSTALLFTLAMVLWWVL
                     IYPVNVELAKWVTGPVPSDWTTYRARWEWGHAIIALTEFLGFAALIAAVLADTPRNSK
                     "
     gene            complement(12180..13352)
                     /locus_tag="VFY74_03155"
     CDS             complement(12180..13352)
                     /locus_tag="VFY74_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5930740.1"
                     /translation="MSAAPQPNGANARRDPGFFRRMWAMVVKEFVQMRRDRMTFATMI
                     IVPIMQLVLFGYAINTDPKQLPTAVLTRDDGPLTRAVLAAMKNTDYFDFKVQVRDPET
                     LDRLVRSGEVQFAVEIPASFERDVRRGDRPSVLVIADATDPVATGTAVSALQGLIDTA
                     LRRELRGPDDELARTAAPFAITVQNRYNPEGITQYNIVPGLLGVVLTMTMMMFTALAV
                     TREIERGTMESLLAMPIKPVEIMIGKIAPFVLVGFVQMTIIVGAAHVLFGVPIMGSVW
                     LLVALATLFAAANLAVGYTFSTIAENQLQAVQMTFFFFLPNILLSGFMFPFRGMPEWA
                     QAIGEMLPLTHFLRIVRGITLKGTGFADMQSDVLALVLFTLAAMALALMRFRRTLD"
     gene            complement(13349..14275)
                     /locus_tag="VFY74_03160"
     CDS             complement(13349..14275)
                     /locus_tag="VFY74_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5930741.1"
                     /translation="MKEIAIDVNGLTKSFGERTVVSDLSLKVPKGEIYGFLGPNGSGK
                     TTTLRMLCGLLTPDSGQGTCLGYDIRTEADAIKRNVGYMTQRFSLYGDLSIRENLEFV
                     ARVYGVSDPKGAASRAIARLGLASRQDQLAGELSGGWKQRLALGACILPEPRLLLLDE
                     PTAGVDPKARREFWDEIHGLAAEGITVLVSTHYMDEAERCHELAYIAFGELLAQGTAK
                     EVVAQSGLSTWIASGGDLRRLGAALHAREGIDMVAPFGAELHVSGRDRSALDAAIKAA
                     KSRDGAHRWMRGEPSLEDVFILLMNNAKDNFA"
     gene            complement(14272..15261)
                     /locus_tag="VFY74_03165"
     CDS             complement(14272..15261)
                     /locus_tag="VFY74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5930742.1"
                     /translation="MKRIAALVAGIVLAAIMLVLTTFEANEDGEFPGYMEADLVLVGS
                     EQGGRIESLTVEEGDSVKQGDPLFTLESSEQEAAVAATKARVAEAEARLADAKAEVQR
                     PGEIEVLEAALREAKAMRQVATNNLDRARTLFDKGWTTKAQLDDAVAQHDRNQAAVAE
                     AENRIAAAKLPGRSDMIDAAAASAEAARYALAEAEKSLAKRKVVSPTSGTIEEVYFRP
                     GEVVRAGQAVTALLPPRNLKVRFFVDEPDRARLQVDQRVALTCDGCPPDLHAKINFIA
                     REAEFTPPVIFSREQRQKLVYLVEARPDGAAVALTAGQPVTVKLAPGEQMVRR"
     gene            complement(15258..15947)
                     /locus_tag="VFY74_03170"
     CDS             complement(15258..15947)
                     /locus_tag="VFY74_03170"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5930743.1"
                     /translation="MVGSRPLKRKTASEGKRRERRDTRPQEIVAAAFEEFAAKGYAAA
                     RLEDVATRAKVSKGLPYLYFKTKEELFKAVIRSVITPLFDAMRERMLTTDLSCEAFLK
                     GPFLAFIQELVKSRRVLIARLLIAEGHKHPELTKFYYDHVIAKGRETLKAFIDRGIER
                     GEFKPTALRDFPQLIIAPVLLAVVWRTLIERHHHLDTDALLATHIDLLLDAIRAPGHA
                     PHARSGEGEGR"
BASE COUNT         3034 a         5169 c         5175 g         2753 t
ORIGIN      
        1 catcgtatcc tccaaagggt gcatgatagc gggcatcggt tttccgatag gattcatgct
       61 cggggtgaag cggacgcgaa tcgcgtgcgc ggccggacta tagttcggaa cggacgccgc
      121 ctgacaggaa cgagccgacc atgagaacga agaccgcgat cgtcgcaatt gcgctagccg
      181 gcgcgctggc aggcgccggc ctcaccttcg cccgcgatgc gggagatcag aacgccgtta
      241 ccgccgagaa attgatcgcc gaagcccttg ccggcgatcc gagcaaagag gtcaccgcgc
      301 aactctatac cttcccgccg ggcaccgttc tgtcctggca catccatccc gatgctcacg
      361 agatcgccta tgtcatcgag ggcaccctca cctttcagcg ggcgggcgag gcgccggaac
      421 aactcgcggc gggcgcggcc gattatcttg cgccgaatat cgtgcatcgc gggatgaaca
      481 cgaccgacaa gccggtgcga ctgttcgtcg tacgcatcaa gcccaaggac aagccgctcg
      541 tcgaggaagt gtctccgccg gattaaccgg agcgtgggat cagcgccggc tgttcttgcg
      601 ccagagcgtg tatagcccga tcgcccatgc cggcagcgca atcagccagt gaaacaacgt
      661 aacttcgtcg ttgagcctca tgccgagatc gtaaggggtg aacggaccga acacggcctt
      721 tcactgccca accctgagaa ccgcgggtga tgcaagccag tgatgatcgc cccaacagcc
      781 cgcggacgtt ccgacctggc agctgaggga tgcgcatcga tcttctcagg tggtatggaa
      841 aatggcagct taacgctcgg agtctcgtcc ggatcatcgg aaatgcggcg atctatagca
      901 ggaggaaaag atgcccagtc ccagcactga ctcggaaaca accgccatcg aagatgctta
      961 taaagccgag atacaagagc tcttcaaaat cttggtcaaa aacttgatcg gtgcgcgcaa
     1021 ttctcacgaa accgatcagc aatgtctcag caggttcacg gcggggctga acgtgacgca
     1081 gcgtgcaagg cagttggccc tttccgccgc gacaccagtc gtgcggacag cagcattagg
     1141 gcgtaagaaa ccgaaggcaa gatagtctcg agtggggatc accatgacgt ccggttctca
     1201 ccgagcgaat ggcggagatc ggcgcggcga acgcggaata gctggtcgaa gagtgccgtc
     1261 gccgcaatag gcacgacgtc agcgccgccc gcttctccgc cagagcgtgt acagcccgat
     1321 ggcccaggcc ggcagtgcga tcagccagtg aaacaacgta acttcgtcgt tgagcctcat
     1381 gccgagatcg taagaagtga acgagccgag cgccgcaagc gcggtaaaga acgcggcgaa
     1441 ggcgagcacg aagccgagcg catagcgggg acggaagaag ccgatatagg tcccggccgc
     1501 gccccatagc agttggaccg cggcgaggta gggatcgatg cggaaaccga gcgcgaagct
     1561 gtcgggatgg ctgtcgaagc cgtaataggc ataggccaac agaaccagcg cgccctgcgc
     1621 cagcaaatag acgctgagcc gcgccgccca gaccacaggc ggccaagttg tttctgcagg
     1681 cgtgtcgctt gccggaggtt cggtcatggc gccccgccgc caagcttatc cgaaaggccc
     1741 tgatggggtc tacgcgcggc tgcccttgcg cttgcccccg attgcattta accaacctct
     1801 cgcgttggtg cgagcctgcg ggcgctaagc cgaggctgta gaggaagggg tattcatggc
     1861 gcgcagtcgt atagcgttga ttggcgccgg ccagatcggc ggcacgcttg cccatctcgc
     1921 cggcatcaga gagctcggcg aaatcgtcct gttcgacgtg gtcgacggtg tgccgcaagg
     1981 caagggcctc gacctgtctc aatccgcgcc cgtcgagggc tatgtcccga aattcaaggg
     2041 caccaacgaa tacgccgagc tgaaggacgc cgatgtcgtg atcgtcaccg ccggcgtgcc
     2101 gcggaagccc ggcatgagcc gcgacgacct gctcgagatc aacctcaaag tcatggagca
     2161 ggtgggcgcg gggctgcgca aatacgctcc cgaagccttt gtcatctgtg tcaccaaccc
     2221 gctcgacgcc atggtgtggg cgttgcagaa aacgagcgga ctgccgcgca acaaggtggt
     2281 cggcatggcc ggcgtgctcg acgcggcgcg tttccgcttc ttccttgccg aggaattcga
     2341 cgtgagcccc gaggacatcc acacgatggt gatgggcggt cacggcgata ccatggttcc
     2401 actcatccgc tactcctcgg tcgccggcat cccgctcgcc gacctcgtca agatgcgctg
     2461 gatcacccag aagcggctcg acgacatcgt gcagcgcacc cgcgacggcg gcgccgagat
     2521 cgttggcctg ctcaagaccg gatcggcctt ttacgcgccg gccgcggccg cgctcgagat
     2581 ggcggagtcc tatctcaaga accaaaagag agtgctccct tgcgcggcct atctgaacgg
     2641 cgagtatggc gtgaagggga tttatgccgg cgtccccatc atcatcggcg ccaagggcgt
     2701 cgagcgcgtc atcgagctcg acctcacttc ggcagagaag gatgccttca tgcactcggt
     2761 cggatcggtg agggcgctcg tcgagctgtg ccagcggatc gcgccgagcc tcgccaccgc
     2821 ttaggccctc aacccgatcg cgagcggcgc cggccgaaaa aactcggacg gcgatcgtca
     2881 ccatgaatat ccacgaacat caggccaaga cgctgctgaa ggcgtatggc gcgccggttg
     2941 ccgacggccg cgtggcggcg agcgcacaag aggcggcgga tcacgcccgc gctctcggcg
     3001 gcgatatctt cgtggtgaag gcgcagattc acgccggcgg ccggggcaaa ggcggcggca
     3061 tcaagctcgt caagacgctt tcggaacttc acgccgaggc cgaccgcatg ctcggcatga
     3121 cgctggtcac gccccagacc gggcccaagg gcaggctcgt gcgtcacgtt tacgtggtca
     3181 aggacaccgt catcgcgcgc gagctttatc tctcgctcct ggtcgatcgc ggctccggcc
     3241 gggtcgcctt tgtcgcctca acggaaggcg gcgtcgacat cgaggccgtg gccgccaata
     3301 cccccgagaa gatcgcgacg ctgacagtcg atcccgcttc gggcatcagc gacttccacg
     3361 ggcgaaccat cgcctcggcg cttgggctcg aaggttcgct cgtcaagcaa tgcggaagcc
     3421 ttgtcaaatc gctctaccgg ctgttcgttg agaaggacgc aagcctgatc gagatcaacc
     3481 cgctggtggt gacgaaggaa ggcaacctcg tctgcctcga tgcaaaggtc gccttcgacg
     3541 acaatgccct cttccgtcac gacgacatcg cggctttgcg cgatattcac gaggaagacc
     3601 cggccgaggt cgaggccgcc aatctcggcc tctcctacgt gaagctcgat ggctcgatcg
     3661 gctgcatggt caacggcgcg gggctggcga tggccaccat ggacatcatc aagcactacg
     3721 gcggcgagcc cgcgaatttc ctcgatgtcg gcggcggcgc cgaccaggag cgcgtcaccg
     3781 aggcattcac catcattctc tccgacccgc atgtggaggc cgtgctcgtc aatatcttcg
     3841 gcggcatcat gcgctgcgac accatcgccg agggaatcat cgcggccgcg cgggagacgc
     3901 atatcgccgt gccgttgatc gtgcggctcg agggcaccaa tgtcgaacgc gggaaggagt
     3961 tgctttctca gtccggcctc gccatcacgt ccgccgacga tctcgacgat gcggccaaga
     4021 aggcggttgc cgcggtgaaa tcttgaccgt taacacagtt acacgtatcc tgtgtgctat
     4081 gaagaatgtc actttggcca ttgacgaaga ggttctgcgc gctgcgcgcc gctacgcagc
     4141 caaccgcgac accacggtga acgcgcttgt acgcgactat ctgacgagcc tcgtcgcttt
     4201 tgaggagaag gccgccaagg cgcgtgagcg cctcgtcgag cttagtgaaa catcagaagg
     4261 tcgtatggga tcttggaagt ggaagcgggg agatgcttac gagggtcgat tgtttcctcg
     4321 acacgaacgt cctgatctac gcggctttgg ggagggggca acaggagaag aagagggcaa
     4381 cggccgctga cctgatccgc aaaacgaagt tcggtctgtc agcgcaagcg ctccaggagt
     4441 tctacacggt cgtaattcgc aaacctgatc gcccactgac ccccgtcaaa gcaatggagt
     4501 ggatcgaggc gttcgaacaa caaccctgcg tctcaacaga cctatctctg gtgaagaccg
     4561 caatcgagat atcgcagcgt ttccgcctcg actattggga cagtgcgatc ctcgcagcgg
     4621 cagagcggtt gggagccgag atcgtctata ccgaggacct caatcacggc cagagctacg
     4681 gctcggtccg tgtcatcaat ccatttctct aggtcatctg cgcacgtcca tgtccgtcct
     4741 tgtcgataag aacacgcgcg tcatctgcca gggcttcacc ggcgcgcaag gcacgtttca
     4801 ctccgagcag gcgattgcct acggcacgcg catggtcggt ggcgtgacgc ccggcaaggg
     4861 tggcagcacg catctcggat tacccgtgtt cgacacggtc aaggacgcgg tggaggaaac
     4921 cggggccgac gcgagcgtgg tctatgtgcc ggcccccttc gcggccgatg ccatcctcga
     4981 agcgatcgat gccgccattc cccttgtcgt ctgcatcacc gagggagtgc cggtgctcga
     5041 catggtcaag gtcaagcgcg cgctctgcgg ctcgaccacg cggctcatcg gtccgaactg
     5101 ccccggcatc atcacgcctg gtcaatgcaa gatcggcatc atgccgggcc atatccacac
     5161 gccgggcaag gtgggcatcg tctcgcgttc gggcaccctg acctatgagg ccgtggctca
     5221 gacgaccgcc gtcagactgg gtcagtcgac ctgcatcggc atcggcggcg atcccgtcaa
     5281 cggcacgaac ttcgtcgatg cgcttgccct gttcctcgcc gacgacgaga ccgaggcgat
     5341 catcatgatc ggcgagatcg ggggcagcgc cgaggaggat ggcgccgagt tcctggtcga
     5401 ggcgggcaac acgaagcctg tggtcggctt tgtcgcgggc gcgacggccc cgcccggccg
     5461 gcggatgggc catgccggtg ccgtgatttc aggcggtaaa ggcgatgcta agcacaaagt
     5521 tgaagcaatg cgcgacgccg gaattgtcgt ggcaaactcg cccgcggccc ttggaacaac
     5581 cctcgcacgg ctattacatg acgattgaaa gccgcgccta acgctcgcct tgtttgccct
     5641 gcaagccggt agacttaaaa gaccggcagg ttcgactcga tgacaaaaca tgaactgaac
     5701 gaggtgtttg ccagcacgtc gttcttgcag ggcgcgaacg ccacctatct cgcagagctc
     5761 cacgcccgtt acgaggcgga tcccgcctcg ctcgatcccg attggcgcgc gttcttcgca
     5821 agtctcgaag acaacaaaga cagcgtgctc gcggaagcgc gcgggccgtc ctggggtccc
     5881 gcacccgcgg cggtggcgct tccggcgagg ccgctcggcc cgccgggcga gcgcgaggcg
     5941 cttgccgcgg cgcgcgacac gctcgccgcc cgcatgctga tccgcaacta tcgcacccgc
     6001 gggcacctcg ccgcgaagct cgacccgctc gaactcgttg agcgccgcga gcatcccgaa
     6061 ctcaggccga agacctacgg cttcgccaaa tcggattacg accgcccgat ctatatcggc
     6121 ggcgtgttcg gtctcgaatt cgcgacgctc cgcgagatcc tcgacatcct gaggcgcacc
     6181 tatagcggcc atatcggcgt cgagtacatg cacatctccg atcccgagca gaggtcgtgg
     6241 attcaggagc gcatcgaagg gcgggaggcc ggcttcacga caaaaggcaa gaccgcgatc
     6301 ctgaagaagc tgatcgaggc cgagaccttc gagcacttcc tcgatgtgaa atacaccggc
     6361 accaagcgct tcggtctcga tggcggcgag tcgatggtgc cggcgctcga gcagatcatc
     6421 aagcggggcg gccaactcgg cgtgaacgag atcgtgatcg gcatgccgca tcgcgggcgg
     6481 ctgaacgtgc tcgccaacgt catggccaag ccctaccggg caatcttcaa tgagttcagg
     6541 ggcggatctg cccatcccga cgacgtcgag ggttcgggcg acgtcaagta ccacctcggg
     6601 gcttcgtcag accgcgagtt cgatctcaac cgcgtgcatc tgtcgctgac cgcgaaccct
     6661 tcgcatctcg agatcgtcga tccggtcgtg ctcggcaaag tccgcgccaa gcaggatcag
     6721 cgcgaagaca gggaacgcaa agaagttctg ccactgctgt tgcacggcga tgcggccttc
     6781 gccggacagg gcgtggtcgc ggagtgtctg ggtctctcgg gcttgcgcgg ccatcgcacc
     6841 ggcggctcgc tccatttcat cgtcaacaat cagatcggct tcaccaccgc gccgcgtttt
     6901 tcccgctctt cgccctatcc gtccgacgtc gccaagatga tcgatgcgcc gatcttccac
     6961 gtgaacggcg acgatccgga ggccgtggtt tacgtggcca aggtcgccac cgagttccgc
     7021 cagcgcttcg gcaagccggt ggtcatcgac atgttctgct accgccggca cggccataac
     7081 gaggccgacg agccggcctt cacccaacct ttgatgtacg cccgcatcgc cgagcatccg
     7141 agcgtggtcg aagtctattc ccggaggctc atcgacgaag gcctcctgac ccgcgaagag
     7201 gtcgacgcca tgcaggccga gttccgcgcc tttctcgagg aggaattcgc ggcgggcgat
     7261 tcctaccggc cgaaccgcgc cgactggctc gacggcagat ggtccggcat cggccacgcc
     7321 gaagaaggcg cgcgccgcgg cgacaccggc gtcgatctcg agcggctgcg cgaggtcggc
     7381 cgcaggatca cgtcgttccc caagagcttc actacgcaca agaccattgc ccgcatcatg
     7441 gccgcccgcc gcaagatgat cgatgagggc gtgggcattg attgggccac ggccgagacg
     7501 ctcgccttcg gcacgctcct cgtcgagggc tatcgcgtca ggctctcagg ccaggactcc
     7561 gagcgcggca cgttctccca gcgccatgcc gtgctgatgg atcaggagag cgagaagaaa
     7621 tacacgccgc tcaagcatgt cgccaaagac caggcgcggt tcgaagtggt caattcgatg
     7681 ctgtccgagg aagccgtgct cggcttcgaa tatgggtaca gcctcgccga gccgaacgct
     7741 ctcgtcctgt gggaagcgca gttcggcgac ttcgccaatg gcgcacaggt catcatcgac
     7801 cagttcattt cctccggcga gcggaaatgg ctccgcatgt cggggctcgg tctgctcctg
     7861 ccgcacggct atgagggcca gggtcccgag cattcctcgg cacggctcga gcgctatctc
     7921 cagctctgcg ccgaggacaa ttggcagatc gcgaactgca ccacgcccgc caactatttc
     7981 cacattctgc ggcgccagct ccatcgccag ttccgcaaac ccctggtgct gatgacgccg
     8041 aaatcgctct tgcgtcacaa gcgcgtggtc tcgacgctag cgcagttcgg ccccggctcg
     8101 agttttcatc gtctcctatg ggacgacgcc cagtttctct ccgatcaggc gatcaagctc
     8161 gtcccggacg acgcgatccg ccgcgtggtg atctcgtccg gcaaggtcta ttacgacctc
     8221 tacgaggagc gcgagaagcg cgggctcgac gacgtctatc ttctcaggat cgagcagctc
     8281 tatcccttcc cggcgcgcgc gctcattcac gagctcggcc gcttcaggga cgccgaaatg
     8341 gtgtggtgcc aagaggagcc gaagaacatg ggggcctgga gcttcatcga gccgaacctc
     8401 gaatgggtgc tcgagcatat cggcgcgagg cacaggcgcc cgcgctatgc cgggcgacca
     8461 agctcggcgg ccaccgcgac cggcctgatg aacaagcatt ttcatgagtt gaaggcattt
     8521 gtcgaagatg cgctgggcta aaccgactga ggagagtgat ggcgactgag atcagggtgc
     8581 cgagcctagg cgaatccgtc gtcgaggcga cggtcggcca gtggttcaag caggccggcg
     8641 acacggtcgt ggccgacgaa cctctggtcg agctcgagac cgacaaggtg acgatcgagg
     8701 tgccagcgcc cgtttccggc gttctctccg agatcttggt caagtccggc gctaccgtcg
     8761 ccgtcggcgc ccttctcggc gcgatcggcg acggcgccgg ggcgatcaaa ccggccaagg
     8821 ccaagcggcc gccggcctcg tcagacctcg ccatggcgga agcgagcgct gtcgctgacg
     8881 agccgtcggt acagccgccg aaggcgccgc cgaccgaacc gaagaagctc gagcgcgagc
     8941 cgaccccgcg ccaggaggtc ggcgtggaag agaggccccc ttcacccgcg gcgcgcaagc
     9001 tcatgatcga gtcagacctt gggggcgatc aggtgatcgg ctcgggccgc cacgggcaag
     9061 ttctgaaagt tgacgtggcc aaggccgtgg aggcgctgct gaagcacgag ccgaaggaga
     9121 aggtcccgcc cccgccccgc gcgcccgtcg cctccgacga cgctgcacgc gaggagcgcg
     9181 tgcgcatgac gaggctccgc cagaccatcg ccaagcgtct caaggaggcg caagacaccg
     9241 ccgccatgct caccacgttc aacgacgtgg atatgggcgc tgtgatgcag ctccggcacg
     9301 attatcgcga cgctttcgag aagcggcacg gggtcaagct cggcttcatg gggttcttcg
     9361 tcaaggctgc ggtgcaggcg ctgaaggaca tccccgcggt caatgccgag atcgacggcg
     9421 aggacatcgt ctacaagaac tattatcaca tcggcattgc cgtcggcaca gagcggggcc
     9481 tcgtggtgcc ggtgctgcgc gacgccgatc gcatgagcct tgccgagatc gagacggcga
     9541 tcgccgattt cggcgcccgc gcccgcagcg gcaagctcac ccttgaggag ctgcaaggcg
     9601 gcaccttcac catctcaaac ggcggcatct acggttcgct gctctcgacc ccgatcctga
     9661 atgcgccgca atcgggcatc ctcggcatgc atcggatcga tgagcgcgcc gtcgtgcgcg
     9721 atggcgcgat cgtggcgcgg ccgatgatgt atctcgcgct ctcctacgac caccgcctcg
     9781 tcgacggcaa ggacgccgtc accttcctcg tccatgtcaa ggacgcgctc gaggacccga
     9841 aacgcctcgt gctcgacctg tagaaacccc tctcccttga tgggagagcg tgccatgcgg
     9901 cgcaagccgc atggcgggtg agggtgacaa acgccgcgcg ctggattacg cctcaaccga
     9961 attggagtgc aggcgatgcc ccaatacgac ctgatcgtca tcggcaccgg acccggcggc
    10021 tatgtctgcg ccatccgcgc ttcgcagctc ggcatgaagg tggccgtggt tgagaagcgc
    10081 gccacgcatg gcggcacgtg cctcaatgtc ggctgcattc cgtcgaaggc gctgcttcac
    10141 gcaagcgagc gctacgcgga agcggaggac ggcttcgccg ccatggggat cagggtgaag
    10201 cccgctctcg acctcgccgc catgctcgcc ttcaaggatg aaggcgtgaa aggcaatgtc
    10261 gacggggtcg cctacctctt gcgcaagcac aagatcgatc actttcacgg gaccggtcgt
    10321 gtgctgggat cgggcaaagt ggaggtcacc ttcatcaacg gcgagaaaca gccggtggag
    10381 gcgaagtcga tcgtcatcgc gaccggttcg gaggcgatgg acctgcccgg gatcgccatc
    10441 gacgagaagc gtatcctctc ttcgaccggc gcgctttcgc tgccggaggt gccgaagcgg
    10501 atggccgtca tcggcgcggg ctatatcggg cttgagctcg gctcggtgtg gtcacgtttg
    10561 ggcagcgaag tcaccgtgat cgaaatgctc gatcgcatcg cgccggggct cgacggcgag
    10621 gtggcaaagc agctccagcg cacgctcgcc aaacagggtc ttgtcttcaa gctcggaacc
    10681 aaagtcgtga gtgccgacag caacggcggg gacctcaagc tggcgctcga gccggcaaaa
    10741 ggcggcaaga ctgagacgct cgaatgcgac gtggtgctgg tcgccgtcgg ccgcgtgccc
    10801 aacaccgaag gcatcggcct cgcggaagcc gggctcacca ccgacaatcg cggccggatc
    10861 gtcgtcgacg agaacttcgc cacgaacgtg cccggtgtct acgccatcgg cgacgtgatc
    10921 agaggcccga tgctggcgca caaggcggaa gaggaaggcg tggcgctcgc ggagattctc
    10981 tcagggcagg ccgggcacgt cgattacggc acgatcccga gcgtcatcta taccgcgccc
    11041 gaggtcgcct cggtcggcaa gacggaggag gagctgaaag aggccggcgt cgcttacagg
    11101 aagggcaagt tccccttcac cgccaacggc cgcgccaagg tcaaccgcac caccgagggc
    11161 ttcgtcaaga tcctcgccga ggaagtgagc gaccgcgtgc tcggcgtgca catcatcggt
    11221 gccgatgccg gaacgatgat cgcggaagcc gcggtgctga tggcgttcgg cggctctgcc
    11281 gaggatctcg cgcgcatttg ccacgcccac cccacgctga acgaggcgat caaggaagcg
    11341 gcgctcgccg tggacgagcg cccgatccat atgtgagcgg cggcgtcgcg tcctgacgat
    11401 tcgctcaaca attgaatccg tcatgcccgg ccgaagtgcc gggcatccac gaattacgtg
    11461 caaggtggaa gacgtggatg gccgggacaa gcccggccat gacgcgtttt cttggatgag
    11521 agcccgtact tttacttcga attgcgcggc gtgtcggcga gcactgcggc gatcagcgct
    11581 gcaaagccca aaaactccgt cagcgcgatg atcgcgtgtc cccactccca gcgggcgcgg
    11641 taggtggtcc agtcggaagg cacgggcccc gtgacccatt tcgccagctc gacattgacc
    11701 ggatagatca gcacccacca taggaccatc gcgagcgtga acagaagtgc ggtcgagagc
    11761 gcgagcccga atccgggtcg cccatgctcg cgcgtgaaat aggcgagaag ggccagcgca
    11821 atgatcgttg ccacgaagat caccgcgccc acagagccga acagattata gagcccgccc
    11881 tggaccgtcg agccgaccca cagatactgg tcccatccca tgcgcgaggg gagctgcatc
    11941 agatggcaga aggcgagccc gagcgtgagc gcggcaagca tgatcgccac gaagcgtgtt
    12001 cccttgagca gcatggcaac cccctctctt gtccttgccc gggttgcttg ccttgccgaa
    12061 gcgaagcttg gcttcgcgga ggccggaccg ggcaatccag tcatcccagt gtctcttcct
    12121 gttgcagggg gttactggat tccctctttc gcagaatgac aaatcgctcg cgccgatccc
    12181 tagtcgaggg ttcggcggaa acgcatcagc gcgagcgcca tcgccgccaa cgtgaagagg
    12241 acaagggcaa gaacgtcgct ctgcatatcg gcgaagcccg tgcctttcag cgtgatgccg
    12301 cgcacgatgc ggaggaaatg ggtgagcgga agcatctcgc cgatggcctg cgcccattcc
    12361 ggcataccgc ggaacgggaa catgaagccg gacagcaaaa tgttcggcag gaagaagaag
    12421 aatgtcatct gcaccgcctg gagctggttc tcggcgatgg tggagaaggt gtagcccacg
    12481 gcgaggttgg cggcggcaaa gagcgtcgcg agcgcgacca ggagccagac gctgcccatg
    12541 atcggcacgc cgaacaggac atgcgccgcg ccgacgatga tcgtcatctg cacgaagccg
    12601 acgagaacga agggcgcgat cttgccgatc atgatctcga ccggcttgat cggcatggcg
    12661 agcaggcttt ccatggtgcc gcgctcgatc tcgcgcgtca cggcgagcgc ggtgaacatc
    12721 atcatggtca tggtcagcac cacgccgagc aggcccggca cgatgttgta ttgggtgatg
    12781 ccttcggggt tgtagcggtt ctgcacggta atggcgaagg gcgccgccgt ccttgcgagc
    12841 tcgtcatcgg gcccgcgcag ctcgcgcctg agcgccgtgt cgatcaggcc ttgcagcgcc
    12901 gacaccgccg tgccggtcgc cacggggtcg gtcgcgtcgg cgatgaccag cacggaaggg
    12961 cggtcgcccc gccgcacgtc gcgctcgaag cttgccggga tctcgacggc gaactgcacc
    13021 tcgcccgagc gaaccagccg gtcgagcgtc tccggatcgc gcacctggac cttgaagtcg
    13081 aaataatcgg tgttcttcat cgccgcgagc acggcgcgcg tcagcggccc gtcgtcgcgg
    13141 gtgagcacgg cagtcggcaa ctgcttggga tcggtgttga tcgcgtagcc gaacagaacg
    13201 agctgcatga tcggcacgat gatcatggtg gcgaaggtca tgcggtcgcg gcgcatctgg
    13261 acgaactcct tgacgaccat cgcccacatg cgccggaaga agcccggatc gcgccgcgcg
    13321 ttcgcgccgt tcggttgagg cgccgcactc atgcgaaatt gtccttggcg ttgttcatga
    13381 gcaggatgaa cacgtcctcc agactcggct cgccccgcat ccagcgatgg gcgccgtctc
    13441 ggcttttggc ggccttgatg gcggcgtcga gcgcggaccg atctcttccg gagacgtgca
    13501 gctccgcgcc gaacggagcc accatgtcga tgccctcgcg ggcgtgaagc gcggcgccca
    13561 gccggcgcaa atcgccgccc gaggcgatcc aggtgctcaa gccggattgc gcgacgacct
    13621 cctttgccgt cccctgcgcc aagagctcgc cgaaggcgat ataggcaagc tcgtggcagc
    13681 gctcggcctc gtccatgtaa tgggtggaaa cgagcacggt gatgccctcg gcggcgagac
    13741 catggatctc gtcccagaac tcgcgccgcg ccttgggatc gacgcccgcc gtcggctcgt
    13801 cgagaaggag aagccgcggt tcggggagga tgcaggcgcc gagcgccagc cgctgcttcc
    13861 atccgcccga caattcaccc gcgagctgat cctgccggct cgcaagcccg agccgcgcga
    13921 tggcgcgcga cgccgcgccc ttggggtcgc tgacgccata gacgcgcgcg acgaattcga
    13981 gattctcgcg gatcgacaga tcgccgtaga ggctgaagcg ctgcgtcata tagccgacat
    14041 tgcgtttgat cgcgtccgcc tcggtcctga tgtcgtagcc gaggcaagtg ccttggcctg
    14101 aatcgggcgt gagcagtccg caaagcatgc ggagcgtggt cgtcttgccg ctgccgttcg
    14161 gaccgagaaa gccgtagatt tcgcccttcg gcactttcag gctcaaatcg ctaaccacgg
    14221 tccgctcgcc aaaactcttg gtcaggccgt tcacgtcgat ggcgatctcc ttcatcgacg
    14281 caccatctgc tcgccgggcg cgagtttcac cgtcaccggc tggcctgcgg tcagcgcgac
    14341 ggcggcgccg tcaggccgag cctcgacgag atagacgagc ttctgccgct gctcgcgcga
    14401 gaagatcacg ggcggcgtga actcggcctc gcgcgcgatg aagttgatct tggcatggag
    14461 gtccggcggg cagccgtcgc aagtgagcgc aacgcgctga tcgacctgaa gcctggcgcg
    14521 gtcgggctca tcgacgaaga agcggacttt cagattgcgc ggcggaagca gcgcggtcac
    14581 cgcctggcct gccctcacca cctcgcccgg cctgaaatag acctcctcga tcgttccgct
    14641 cgtgggcgat accaccttgc gcttggcgag gctcttctcg gcctcggcga gggcgtagcg
    14701 cgcggcctcg gcgcttgcgg cggccgcatc aatcatgtcc gaccggccgg ggagcttggc
    14761 cgcagcgatg cgattctcag cctcggccac ggcggcctgg ttgcggtcgt gctgagcgac
    14821 ggcgtcgtcg agctgcgcct tcgtggtcca tcctttgtcg aagagggttc gcgcgcggtc
    14881 gagattgttg gtcgcgacct gtcgcatcgc cttggcctcc ctgagcgcgg cctcgagcac
    14941 ttcgatctcg ccgggccgct gcacctccgc cttcgcgtcg gcgagccgcg cctcggcctc
    15001 ggcgacgcgc gccttcgtcg ctgccaccgc tgcctcctgc tcggagcttt ccagcgtgaa
    15061 aaggggatcg ccttgcttga cgctgtcgcc ttcttcgacg gtaagggact cgatgcggcc
    15121 tccttgctcc gaacccacga gaacgaggtc cgcctccatg tagccgggaa attcaccgtc
    15181 ctcgttggcc tcaaaggtcg tgagcacgag catgattgcg gcgagaacga taccggcaac
    15241 gagggcggcg atacgtttca tcgcccctct ccttcgccgc tcctggcgtg cggcgcatgc
    15301 cccggcgccc tgatcgcgtc gaggagaaga tcgatatgcg ttgcaaggag cgcgtcggta
    15361 tcgagatgat gatggcgctc gatcagggtg cgccagacga cggcaagcag gaccggggcg
    15421 atgatgagct gcggaaagtc gcgtaacgcc gtcggcttga attcgccgcg ctcgatcccg
    15481 cgatcgatga aggccttaag cgtctcccgc cccttggcga tgacgtggtc gtagtagaat
    15541 ttcgtaagct ctgggtgctt gtggccttcg gcgatgagca agcgcgcgat cagcacgcgg
    15601 cgcgacttga cgagctcctg gatgaaagcg aggaacggac ctttgagaaa cgcctcgcac
    15661 gacagatccg tcgtcagcat gcgctcgcgc atggcgtcga agagcggcgt gatgacgctg
    15721 cggatgacgg ccttgaacag ctcctccttg gtcttgaagt acagataggg caggcccttg
    15781 ctcactttgg cgcgtgtggc cacgtcttcg agcctggccg ccgcgtagcc ctttgccgcg
    15841 aactcctcga aggcagcggc gacgatctcc tgcgggcgtg tatcccgccg ctcgcgccgc
    15901 ttgccttcgc tcgccgtctt gcgcttgagc ggtctcgaac cgaccatcgc cttgtcctca
    15961 cctccgccaa ttcccgctcc aatttatgac tgactggtca gtactgcaag ggttggaggt
    16021 gaaatttcat ctgccctgca tctctccgtc atgcccggac cctcgggtct ttgcccgagg
    16081 gcaggcttga tccgggcatc cattccgtga cccagagcca agtcgaggcg t
//
LOCUS       DASUQP010000069         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_711276,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000069 DASUQP010000000
VERSION     DASUQP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711276"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1507
                     /gene="mtaB"
                     /locus_tag="VFY74_03175"
     CDS             254..1507
                     /gene="mtaB"
                     /locus_tag="VFY74_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX5930744.1"
                     /translation="MPDLDIITFGCRLNAYESEVMRAQAAAAGLFDAVIVNTCAVTAE
                     AVRQAGQAIRKARRERPEARIVVTGCAAQIDPSRFAAMDEVDHVIGNREKLEAETFAR
                     FGVDGGERVVVNDIMSVRETASHLIDGFGTRARAYVQIQNGCDHRCTFCIIPFGRGPS
                     RSVPAGEVVAQVRRLIEKGYAEIVLTGVDITAYGKDLPGEMTLGKLVRSVLKLVPELP
                     RLRLSSIDSIEADAALLKAIAEEERLMPHLHLSLQSGDDLTLKRMKRRHARADSVRFC
                     HEVRRVRPDMVFGADLIAGFPTETESMFANSLSLVDECGLTFLHVFPYSAREGTPAAR
                     MPQLHGAIIAKRAGALREKGVAALKRHLQSAKGRRIMVLVENEGSGRAADFTPVRLKT
                     EARAGSLVEAVVAGDNGTSLIAVRS"
     gene            1534..2478
                     /gene="ftsY"
                     /locus_tag="VFY74_03180"
                     /pseudo
     CDS             1534..2478
                     /gene="ftsY"
                     /locus_tag="VFY74_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
BASE COUNT          436 a          844 c          848 g          414 t
ORIGIN      
        1 atgagggaga gggccggcgc gcgtagcgcg acgcaaggcg ggaagggtga gggcgaaatc
       61 tttttcacgc aggcttcgcc ctcggccccc tcaccgcgcg ccaagtggcg cgcgtgctct
      121 ccccaagggg agagcggtaa taggtggcgt ttcgatacag caggcgcttc ggcttcgctc
      181 aggcaggcgc cggtatgagg gagaaccagg cgtggggtct gactcgcccg ccgtttcatg
      241 ttagaagccg ccaatgccgg atctcgacat catcaccttc ggttgcaggc tcaacgccta
      301 cgagtccgaa gtcatgcgcg cgcaggccgc cgctgccggg ctctttgacg ccgtcatcgt
      361 caatacgtgc gccgtgacgg ctgaagccgt gcgtcaggcg ggtcaggcga tccgcaaggc
      421 ccgccgcgag cggccagagg cgaggatcgt cgtcaccggc tgcgccgccc agatcgatcc
      481 ctcccgtttc gccgcgatgg acgaggtcga tcatgtgatc ggcaatcggg agaagctcga
      541 ggccgagacg ttcgcccgtt tcggcgtcga tggcggcgaa cgcgtcgtgg tcaatgacat
      601 catgtcggtg cgcgagacag cgtcgcatct gatcgacggc tttggcaccc gcgcccgcgc
      661 ctacgtgcag atccagaacg gttgcgatca ccgctgcacc ttctgcatca ttcccttcgg
      721 gcgcggaccg tcgcgctcgg tgcctgcggg cgaggtggtg gcgcaagtgc ggaggcttat
      781 cgaaaagggc tacgccgaga tcgtgctgac cggcgtcgac atcaccgcct atggcaagga
      841 ccttcccggc gaaatgacgc tcggcaagct tgtccgctcc gtgctcaagc tcgtgccaga
      901 gcttccgcgc ctcaggctgt cctccatcga ctcgatcgag gccgatgccg ccctgctcaa
      961 ggcgatcgcc gaggaggagc ggctaatgcc gcatctgcat ctctcgttgc agtccggcga
     1021 cgatctcacg ctcaagcgga tgaagcggcg gcacgcccgc gccgattcgg tccgcttctg
     1081 ccatgaagtg cgccgggtcc gccccgacat ggtgtttggc gccgatctca tcgcgggatt
     1141 tcccaccgag accgagtcca tgttcgcgaa ttcgctcagt ctcgtcgacg aatgcgggct
     1201 cacgttcctc cacgtctttc cctattcggc gcgcgaggga acgcccgccg cgcgcatgcc
     1261 gcagcttcat ggcgcgatca tcgccaagcg cgcgggcgcg ctccgcgaga agggcgttgc
     1321 cgcgctcaag cgccatctcc aaagcgccaa gggacgccgc atcatggtgc tggtggagaa
     1381 tgagggaagc ggacgcgccg ccgatttcac gccggtccgc ctcaagaccg aggccagggc
     1441 cggaagtttg gtcgaggcgg tcgtcgccgg cgataacggc acgtccctca tcgcggtgcg
     1501 atcatgagcg atcgcgagag gacggacgag cgagagccga agcagggttg gttcgatcag
     1561 cttcgccgcg ggctcgcccg cacaaccaat gccctgtccg acggtcttgc cggggcgctc
     1621 accaagcgca agttcgacga ggagacgctc gaccggatcg aggaggcgtt gatcaaggcc
     1681 gatctcgggg tcgccatggc ggcgcgcatt cgcgaacggc tggcaagggg ccgctacgac
     1741 aggggcctct cgcccgaggc ggtgcgcgac gtcgtcgccg cagagatcgc aagcgtgctt
     1801 cagcccttgg cgcggccttt cgcacgcgtc actggccagc ggccccatgt ctttctcgtt
     1861 gtcggggtga acggcaccgg caagaccacc accgccgcca agctcgccca tcagttcaag
     1921 cgcgagggcc tgaaggtcat gcttgccgcg gccgacacgt tccgcgccgc agccatcgat
     1981 cagctcaaag tctggggcga gcgggtcggc gccgaggtcg tggccaaggc ggtgggcgcg
     2041 gatcccgccg gcgtcgcctt cgaagccttc gagcgcgcac gcgcggaagc tgttgacgtg
     2101 ctcctcatcg acacggccgg acgcctccag aacaagacgg atctcatggc cgagctcgcc
     2161 aagatcgtgc gcgtgctcaa gaagatcgac cagagcgctc cgcacggcgt catcctcgtg
     2221 ctcgacgcca ccaccgggca gaatgcactc gctcaggtcg agatcttcaa ggagatcgcg
     2281 ggcgtcaccg gtctcgccgt gaccaagctc gacggcaccg cgcgcggcgg catccttgtc
     2341 gccattgccg agcgcttcaa gattcccatt aatgccatcg gcatcggcga gggaatagac
     2401 gatctcaagc cgttcgacgc gcgagacttc gcgcgcgcca tcgcgggagc aaacagccag
     2461 aatgaagccg cggcctgagc gtgacatcgc gaccgcgggc gtgggtgccg cgccgcaaca
     2521 aggggtctgg tccaagctca tc
//
LOCUS       DASUQP010000070         4463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1257102, whole genome shotgun sequence.
ACCESSION   DASUQP010000070 DASUQP010000000
VERSION     DASUQP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257102"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFY74_03185"
     CDS             complement(<1..305)
                     /locus_tag="VFY74_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5930745.1"
                     /translation="MIPGGSSTAEKDGRRAATPLLLLLAGGFAVPLIAVTVFSFMPPR
                     TFSLLQAPTLENYKTIFTGTSYISFLWSVVLAVATVVLLALICYPIAYGLVHVFGRWS
                     "
     gene            complement(302..1375)
                     /locus_tag="VFY74_03190"
     CDS             complement(302..1375)
                     /locus_tag="VFY74_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5930746.1"
                     /translation="MTPQRPTILTLKALRKDFGAFTAVDGIDLKIAEGEFFTIVGPSG
                     SGKTTLIRMMAGMETPTSGDIVLRQTRINDVPANKRPTCMVFQSLALFPHRSVGENIE
                     FAMKIRGVEPAKRKARAVDLLRQLRLPESYYAKSVQKCSGGERQRVALARALAYNPEI
                     LFFDEPLSAIDYKLRKTLEKELKDIHRETGKTFVYITHSLEEAMVMSDRIGVMREGKL
                     VQVGTPNEIYSSPRSKFVSEFMGDVNVIAVKSTGNGSLLAPSLEAQFTAPPGAPEIES
                     GHLVIRPEYVRFLGKADEAANSLSGRLYNEYALGSRIQYQVRVGDQVFVIEKLRQQAY
                     NGKLDDQVLIGWDSQHSILVGEE"
     gene            complement(1495..2649)
                     /locus_tag="VFY74_03195"
     CDS             complement(1495..2649)
                     /locus_tag="VFY74_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PotD/PotF family extracellular solute-binding
                     protein"
                     /protein_id="HEX5930747.1"
                     /translation="MRQRQLTRRNFMQQSATFAAAFAAGGASAFVKPAHAAREKELNI
                     LCWEGYNSAQVLDPFRSATGATVKAESLTNDPTMINRLRAGETNVWDLINVNNPWARK
                     IMLPEKLIKPLDRARFEPYFEKMLPAFKPPYKWAMDDSGKELIGMAQRFGPYSFVVNT
                     DKISRETAEDQGWDLWNDPKLAGKYGILESDDWNVFCICAIAGFDPFKTHNDAQVAKF
                     AETAKRVFNGKKLVGDIATNNQALVSGEIDFHLTGGTYSVSPARADGHMNLRGITPKK
                     GPFAGGKGGIAWIEITSLVNNPNLSPLATEFLAYVQEPKMAHTVAFAEGTYNPVTQMG
                     NPECFKLFTKEELEAIQWDSLEEEMAGSVEYDIVPDYDKLLDIWNAAKKG"
     gene            complement(2920..3615)
                     /locus_tag="VFY74_03200"
     CDS             complement(2920..3615)
                     /locus_tag="VFY74_03200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX5930748.1"
                     /translation="MTWKTAYRSIYYDGAPEPADPDLAKESTALLVIDVQNTYLTRPD
                     RASLSAEEQRRHDAWTPFHERMRDIVIPRTRELLSLFRENGYECLFARIACHTKDGRD
                     RSLSQKMPGWNNLLLPKDEHPSQLATELQPQGDEIVVTKTTDSALTGTNLRLILTNLG
                     IKTVVCCGIFTDQCVSSTVRSLADESFGVVVVEDCCAAGSDELHRKELEIINMIYCHV
                     MSSAELKALMGLG"
     gene            complement(3624..4052)
                     /locus_tag="VFY74_03205"
     CDS             complement(3624..4052)
                     /locus_tag="VFY74_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HEX5930749.1"
                     /translation="MSNERLSIVDYPLAEKRPEVVLGKRGKGLDEITLEALIEDRVTL
                     EDLRITAAALRQQAEIARAAGRDTLAANFERGAELVDVPQDTIMRIYELLRPGRAESR
                     SALLTTAGELREQYGAEGMAAFVEEAAAVYERRGLFKKRF"
     gene            complement(4049..>4463)
                     /locus_tag="VFY74_03210"
     CDS             complement(4049..>4463)
                     /locus_tag="VFY74_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HEX5930750.1"
                     /translation="GITEGGAKARVVRMKHTADTSFLGLSAARLAGSGVGIGIQAKGT
                     AVIHQRDRLPHNNVELFSNSPITRLEHYRRMGANAAAYALGALPEPVVVPTRGEAMGA
                     RYHARVSLIYAIETELTAEGAVPEQVEVAFLGDTA"
BASE COUNT          745 a         1386 c         1463 g          869 t
ORIGIN      
        1 gaccagcggc cgaacacgtg cacgaggcca taggcgatgg ggtagcagat gagcgcaagc
       61 aggacgacgg ttgcgaccgc gagcaccacc gaccaaagga aggagatgta ggaggtcccg
      121 gtgaagatcg tcttatagtt ctccagcgtc ggcgcctgga gaaggctgaa ggtgcgcggc
      181 ggcatgaaac tgaaaacggt gaccgcgatc agcggcacgg caaagcctcc ggcgagcaac
      241 agaagcaagg gggtggccgc gcgccgcccg tccttctcgg ccgtgctgct gccgccggga
      301 atcattcctc ccccaccaga atggaatgct gggaatccca gccgatgagc acctgatcgt
      361 cgagcttgcc gttgtaggcc tgctgacgga gcttctcgat tacgaacacc tggtcgccga
      421 cacgcacctg atactgaatg cgcgagccga gcgcgtactc gttgtagagg cgcccgctca
      481 ggctgttggc ggcctcgtcg gccttgccga ggaagcggac gtattccggc cggatgacga
      541 gatggcccga ctcgatttcg ggcgcgccgg ggggtgcggt gaactgcgct tcgagcgaag
      601 gtgcgagcag ggagccattg ccggtgctct tcaccgcgat gacgttgacg tcgcccatga
      661 actcggagac gaacttgctc cgcggcgagg agtagatctc gttcggcgtg ccgacctgca
      721 cgagcttgcc ctcgcgcatg acaccgatgc ggtcgctcat caccatcgcc tcttcgaggc
      781 tgtgcgtgat gtagacgaag gtcttgccgg tctcccggtg aatgtccttg agctccttct
      841 ccagcgtttt tctcagcttg tagtcgattg ccgagagcgg ctcgtcgaag aacaggatct
      901 cgggattgta ggcgagagcg cgagcgagcg cgacgcgctg gcgctcgccg cccgagcact
      961 tctgcacgct cttcgcgtag taggattcgg gaagcctgag ctggcgtagg agatcgaccg
     1021 cgcgcgcctt gcgcttggcg ggctcgacgc cgcggatctt catggcgaac tcgatgttct
     1081 cgccgacgct ccgatgcggg aacagggcga gcgactggaa caccatgcag gtcggccgct
     1141 tgttggccgg cacgtcgttg atacgagttt ggcgtaaaac gatgtcgccc gaggtgggcg
     1201 tctccatgcc ggccatcatg cggatcagcg tcgtcttgcc gctgcccgac ggcccgacga
     1261 tggtgaagaa ctcgccttct gcgatcttga ggtcgatgcc gtcgaccgcc gtgaaggctc
     1321 cgaagtcctt gcgcagagcc ttcagcgtga gaatggtggg tcgttggggc gtcacgtggc
     1381 tctcacctac gcgatggtcc agatcaggca gcctcccgca tgaccgaggc catgcggaag
     1441 gctgctattt aaataccgtc aatgcgacct ggacaacgcc gatcgagccg acggtcagcc
     1501 tttcttcgcc gcgttccaga tgtcgagcag cttgtcgtag tcggggacga tgtcgtactc
     1561 gaccgagccc gccatctcct cctccaggct gtcccactga atggcttcga gttcctcctt
     1621 ggtaaagagc ttgaagcact ccgggttgcc catttgcgtg accgggttgt aagtgccttc
     1681 ggcgaaggcg acggtgtggg ccatcttcgg ctcctgcacg taggcgagga actccgtcgc
     1741 gagcggggac aggttcggat tgttgaccaa ggacgtgatc tcgatccagg cgatgccgcc
     1801 cttgccgccg gcgaacgggc ccttcttcgg cgtgatgccg cggaggttca tgtgcccgtc
     1861 ggcacgcgct ggcgagacag aataggtgcc accggtgaga tggaaatcga tctcacccga
     1921 cacgagcgcc tggttgttgg tggcgatgtc gccgacgagc ttcttgccgt tgaagacccg
     1981 cttggcagtt tcggcaaatt tcgcgacttg cgcgtcgtta tgcgtcttga aggggtcgaa
     2041 tcccgcgatg gcacagatgc agaacacgtt ccaatcgtcg gattcgagga tgccgtactt
     2101 gccggcgagc ttggggtcgt tccagaggtc ccagccctgg tcctcggcgg tctcgcggct
     2161 gatcttgtcg gtattgacga cgaaggaata gggcccgaag cgctgagcca tgccgatcaa
     2221 ttcctttccg gagtcgtcca tggcccactt gtaggggggc ttgaaggccg gcagcatctt
     2281 ctcgaagtag ggctcgaacc gggcccggtc gagcggcttg atcagcttct caggcagcat
     2341 gatcttgcgt gcccaaggat tgttgacgtt gatcaggtcc cagacgttgg tctcaccggc
     2401 cctcaggcgg ttgatcatcg tcggatcgtt ggtgaggctc tcagccttga cggtcgcacc
     2461 cgtcgcggac cggaatgggt cgaggacctg ggccgagttg tagccctccc aacacaggat
     2521 gttgagctcc ttttcgcgcg cggcatgggc cggcttcacg aacgcggaag ccccgcccgc
     2581 tgcgaacgcg gcggcgaagg tggcggattg ctgcatgaag ttgcggcggg ttaactgccg
     2641 ttggcgcatt gtctgttctc ctggtcgcgg ggcttcgtga ccctaaggtg tgcggaaagg
     2701 cgatggcgtg tcgtccgatg ggcgagcggt tccgccggtc cgggtgtctt ccggcgaggc
     2761 cacgctagga cctgcgcccg gcttcgatcc ggacttgctc gctccgcctc ccttatctta
     2821 cccactcccg gcggccgtgg cacccctgct tcgcgaacct ttggctttgc cgcaccgcgg
     2881 catgcggcct ggagaagggg atgcacttga ccttctcctt cagcctaagc ccatgagcgc
     2941 cttgagctcg gcgctcgaca tgacgtggca atagatcata ttgatgatct cgagctcctt
     3001 gcgatgcagc tcgtccgatc ccgcagcgca gcaatcctcg accacgacga cgccgaaact
     3061 ttcgtcggcc aggctcctga ccgtcgagga cacgcactgg tcggtaaaga tgccgcagca
     3121 caccaccgtc ttgataccga ggttggtgag gatcagccgc agattggtcc cggtgagcgc
     3181 gctgtcggtc gtcttcgtca ccacgatctc atcgccttgc ggctgaagct ccgtggcaag
     3241 ctgagacgga tgctcgtcct tgggcagaag cagattgttc cagcccggca tcttctgcga
     3301 cagcgaccgg tcgcggccgt ccttggtgtg gcaggcgatg cgggcgaaca ggcattcgta
     3361 gccgttctcg cgaaagagac tcaggagctc ccgcgtgcgg gggatgacga tgtcgcgcat
     3421 ccgctcgtgg aagggcgtcc aggcatcatg gcggcgctgt tcctcggcag acagcgaggc
     3481 tctgtcaggc cgggtgagat aggtgttctg cacgtcgatg acgaggagcg ccgtgctctc
     3541 cttggcgagg tccggatcgg caggctcggg cgcaccatcg tagtaaatcg atcgataggc
     3601 cgtcttccag gtcattcgct tgctcagaat cgtttcttga acaagccgcg ccgctcgtag
     3661 accgcggcgg cctcctccac gaacgccgcc atgccctcgg ctccgtattg ctcgcgcagc
     3721 tcccccgccg tcgtcaaaag cgccgaccgc gactctgcgc ggccggggcg caacagctcg
     3781 taaatgcgca tgatcgtgtc ttgcggaacg tctacgagct cggcgccgcg ctcgaagttc
     3841 gcggccaaag tgtctcgacc ggcggcacgg gcgatctccg cctgctggcg caacgccgcg
     3901 gctgtgatgc gcaggtcctc cagcgtgacg cggtcctcga tcagggcctc gagcgtgatc
     3961 tcatcgaggc ccttgccgcg ctttccgagc acgacctcgg gacgcttctc cgccagagga
     4021 tagtcgacga tcgagagccg ttcattgctc atgcggtgtc ccccaggaag gccacctcga
     4081 cctgctcggg cacggcgccc tccgcggtca attccgtctc gatggcatag atcagcgaaa
     4141 cgcgcgcgtg atagcgggcg cccatggcct cgccccgtgt tggcaccacc accggctccg
     4201 gcaacgcgcc gagcgcgtag gcggcggcgt tggcgcccat gcgccgatag tgctcgagcc
     4261 tggtgatcgg ggagttggag aagagctcga cattgttgtg cggcaagcgg tccctctggt
     4321 ggatgaccgc ggttcctttc gcctggatgc cgatgccgac gcccgacccc gcaagacggg
     4381 cggcgctcag gccgagaaac gacgtgtcgg ccgtatgctt catccgcacg acgcgagcct
     4441 tcgcgccccc ttccgtgatg ccg
//
LOCUS       DASUQP010000071         6378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_985008,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000071 DASUQP010000000
VERSION     DASUQP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985008"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VFY74_03215"
     CDS             complement(<1..1292)
                     /locus_tag="VFY74_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX5930751.1"
                     /translation="MGKITGFLEIEREDRAYEPASDRVQHYREFVIAPSEAEIVRQAA
                     RCMDCGIPYCHTGCPVNNQIPDWNDLVYHSDWEVAASNLHSTNNFPEVTGRICPAPCE
                     AACTLNIIDVPVTIKSIECAIADKAFDEGWVQPLPPESATGKRVAVVGSGPAGLAAAQ
                     QLARAGHDVHVYEKYAYPGGLLRYGIPDFKMEKYIVERRVKQMEAEGVTFHCNVHVGR
                     DLAVDTLERDYDAVVLAGGAEHPRDLKVPGHDLDGVHFAMDFLPQQNRRISGEPVGDV
                     APILANGKKVVVIGGGDTGSDCIGTSFRQGAISVTQLEILPQPPDMENKALTWPHWPL
                     KLRTSSSQAEGAARDFSVATNRFLGTNGKVEALECIRLDGQLKPMPGTEFHIPADLVL
                     LAMGFLHPVQEGMVRDLWLDLDARGNVKANELDYRTSRS"
     gene            1367..1600
                     /locus_tag="VFY74_03220"
     CDS             1367..1600
                     /locus_tag="VFY74_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930752.1"
                     /translation="MKAKMIKEILRRAEGWPESAQADLAQAALEIERELQHDTYTATP
                     EEVAAIERGLREADQGKFATEAEVEAVLGKYRR"
     gene            1600..1893
                     /locus_tag="VFY74_03225"
     CDS             1600..1893
                     /locus_tag="VFY74_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5930753.1"
                     /translation="MRVVYTQGALSDLEGILGGLHSINPLVAATVERSIRATIARVAS
                     WPRSARMVTQWEEGNVRAVPLVRYPYLIFYQIKKQRIEILHVRHTSRAPWKAM"
     gene            complement(1906..>6378)
                     /gene="gltB"
                     /locus_tag="VFY74_03230"
     CDS             complement(1906..>6378)
                     /gene="gltB"
                     /locus_tag="VFY74_03230"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX5930754.1"
                     /translation="LVQIPHDFLRAVCAPLNIELPEPGHYGVGQMFMPRNQAQRIYCE
                     SVIERVIEAEGLTLIGWRDVPCDNSCLSASVIATEPVHRQVFIGRGPGIEDETAFERR
                     LFLLRKVISNTVMGETGGREIGFYTVSLSCRTLVYKGMFLAYQLGAYYRDLHHPLFAS
                     ALALVHQRFSTNTFPSWKLAHPYRMVAHNGEINTLRGNLNWMAARQASVSSPLFGDDI
                     EKLWPISYEGQSDTACFDNALEFLVQGGYSLAHAAMMLIPEAWAGNPLMDAKRRAFYE
                     YHACLMEPWDGPAAMAFTDGRQIGATLDRNGLRPARYLVTDEGLVIMASETGVLPIAE
                     KSIVEKWRLQPGKMLLIDLEKGRIISDEEIKAELASAHPYQQWLDRTQIRVHELPGAP
                     PPPPRTNVSLLDRQQAFGYTQESLKFLMMPMAESGQEAVGSMGTDTPLSALSSRPKLL
                     HTYFKQNFAQVTNPPIDPIREELVMSLVTFIGPRPNLLDLEGTSRQKRLEASHPILTN
                     ENLERIRGIGEVADNQFRTVTLDITYGADHGATGMAEALGALCSRAEEAVRAGENIIV
                     LSDRATGPDRVPIPSLLATSAVHHHLIRCGLRTSVGLVVETGEAHEVHQFCTLAGYGA
                     EAINPYLAFETIEAMLPELDEEITAEEAVKRYIKAIDKGILKVMSKMGISTYQSYCGA
                     QIFDAVGLRSDFVAKYFTGTNSQVEGVGLEEIARETVERHELAFSTAPVLREALDVGG
                     EYAYRVRGETHMWRPSVVADLQHAVRGSLPAKYRSYAKQINEQTEQLLTLRGMFRIKT
                     AEEIGRNPIPLDEVEPAKDIVKRFSTGAMSFGSISREAHTTLAIAMNRIGGKSNTGEG
                     GEESDRYRPLPNGDSMRSKIKQVASGRFGVTTEYLINADMMQIKMAQGAKPGEGGQLP
                     GHKVDATIAKVRHSTPGVGLISPPPHHDIYSIEDLKQLIFDLKNVNPTGDVSVKLVAE
                     VGVGTVAAGVAKAMADHVTIAGFEGGTGASPLTSIKHAGSPWEIGLAETQQTLVLNRL
                     RGRIAVQVDGGIRTGRDVMVGALLGADEFGFSTAPLIAAGCIMMRKCHLNTCPVGVAT
                     QDPVLRARFQGKPEHVINFFFFVAEEVREYMATLGFRKFSEMIGQTEILDKGQAIDHW
                     KARGLDFTRIFHKPEVPPEVAVYNSERQDHGLDKVLDVKLIGLAREAIEEKKPVKIDL
                     PIKNTDRTTGAMLSGEIATRYGHTGLPEDTIWVSVSGAAGQSFGAWLAHGVTLDLVGE
                     ANDYVGKGLSGGRIIVRPPADSGIVPEKSIIVGNTVLYGAIAGECYFRGVAGERFAVR
                     NSGAIAVVEGTGDHGCEYMTGGVVVVIGPTGRNFAAGMSGGIAYVLDEDGAFERRCNL
                     AMVDLEPVPSENQVMERSRHQTADLESHGLVDVTDMTRFDEERLHQLVQNHLHYTGSA
                     RAKAILDHWAAYLPKFVKVMPVEYRRALEEMARQQQADKAGLGEIEIGLRGNGK"
BASE COUNT         1105 a         1970 c         2115 g         1188 t
ORIGIN      
        1 gaccgcgagg tgcggtagtc gagttcgttc gccttgacgt ttccgcgggc atcgaggtcg
       61 agccatagat ccctgaccat tccctcctgc acgggatgga ggaaacccat ggcgagcagc
      121 acgagatcgg cggggatgtg aaactccgtg cccggcatcg gcttcagctg gccgtcgagg
      181 cggatgcact cgagcgcctc gaccttgccg ttcgtgccaa ggaaccggtt tgtcgccacg
      241 ctgaaatcgc gcgcggcgcc ctcggcctgg ctcgatgagg tgcgcagctt gagcggccaa
      301 tgcggccagg tcagcgcctt gttctccatg tcgggcggct gaggaaggat ctcgagctga
      361 gttaccgaga tggcgccctg gcggaacgac gtgccgatac agtcggagcc cgtatcgccg
      421 ccgccgatca ccacgacttt ctttccgttc gccaggatcg gcgcgacgtc tccgacaggc
      481 tcgccggaga tgcggcggtt ctgctgcggc aggaagtcca tggcgaagtg gacgccgtca
      541 agatcgtggc ccggaacctt aaggtcgcgg gggtgctcag ccccgcccgc gagtacgacg
      601 gcgtcgtaat cgcgttcgag cgtgtcgacg gcgaggtcgc ggccgacatg gacgttgcaa
      661 tgaaaggtaa cgccttccgc ctccatctgc ttcacccggc gctcgacgat gtatttctcc
      721 atcttgaaat cgggaatgcc gtagcggagc aggccgccgg gataggcgta cttctcgtag
      781 acatgcacgt catgaccggc gcgggcgagc tgctgggccg cggcaagtcc tgctggacct
      841 gagcccacga cggccacgcg cttgccggtc gccgactccg gcggcagcgg ctgcacccag
      901 ccctcgtcga aggccttgtc ggcgatcgcg cattcgatcg atttgatggt caccggcacg
      961 tcgatgatgt tcagtgtgca cgcggcctcg cagggcgcgg ggcagatccg gcccgtcacc
     1021 tcggggaagt tgttggtcga atgcaggttc gacgccgcga cctcccagtc ggaatggtag
     1081 acgaggtcgt tccagtcggg gatctggttg ttgaccggac agccggtatg acaataggga
     1141 atgccgcaat ccatgcagcg ggcggcctgg cgcacgatct ccgcttcgct cggcgcgatc
     1201 acgaactcgc ggtagtgctg gacgcgatcg gacgcgggct cataagcccg gtcctcgcgt
     1261 tcgatctcga gaaagcccgt gatcttgccc attgcgtcct tcagtctcgc ccggctccag
     1321 ccctggcgct gcttaccacc aacctcttat gtgctatcat cggaacatga aagcgaagat
     1381 gatcaaggag attcttcggc gggctgaagg ctggcccgaa agcgctcagg cagacttggc
     1441 gcaagcggcc cttgagatcg aacgcgagct gcaacacgac acctataccg cgacgcctga
     1501 ggaagtggct gcaatcgagc gtggcctccg agaggccgac cagggcaaat tcgcaaccga
     1561 agccgaagtc gaggcagttt tgggtaagta ccgccgctga tgcgggtggt ttacactcaa
     1621 ggcgcactct ctgacctcga aggcatcctc ggcggacttc attcgatcaa tcctttggtc
     1681 gctgctacgg tcgaacgcag catcagagcc acgattgccc gcgtcgcgag ttggccgagg
     1741 agcgctcgca tggttacgca gtgggaagag ggcaatgtgc gggcagtccc gctcgtgcgc
     1801 tatccctatc tgatcttcta ccagatcaag aagcagcgaa ttgagatact tcatgtgcgc
     1861 catacgtcgc gcgcgccctg gaaagcaatg tgatctcagt gggtgtcact tgccgttgcc
     1921 tcgcaagccg atctcgatct cgccgagacc cgccttatcc gcctgctgct gccgcgccat
     1981 ctcctccagc gcgcggcgat actcgaccgg catcaccttg acgaatttgg gaaggtacgc
     2041 ggcccagtgg tcgaggatcg ccttcgcgcg cgccgagccg gtgtagtgga ggtggttctg
     2101 aacgagctga tgcaggcgct cttcgtcgaa gcgggtcatg tcggtgacgt cgacaaggcc
     2161 gtggctttcg agatccgcag tttggtggcg gctacgctcc atcacctggt tctcgctcgg
     2221 caccggttcg agatcgacca tggcgaggtt gcagcggcgc tcgaacgcgc catcctcgtc
     2281 gagaacatag gcgatgccgc ccgacatgcc ggcggcgaag ttgcggcccg ttggcccgat
     2341 caccacgacc acgcctccgg tcatgtactc gcagccatgg tcgcctgttc cttcgaccac
     2401 ggcgatggcg cctgagttgc gcacggcgaa gcgctcgccg gcgacgccgc ggaaatagca
     2461 ctcgccggca atcgcgccgt aaagcacggt attgccgacg atgatcgatt tttccggaac
     2521 gatgcctgaa tccgccggcg ggcggacgat gatgcggccg ccggagaggc ccttgccgac
     2581 atagtcgttg gcctctccga caagatcgag ggtgacgcca tgtgcgagcc aggcgccaaa
     2641 actctgcccc gcggcgcccg acacgctcac ccagatcgtg tcctcgggca gacctgtgtg
     2701 gccgtagcgc gtggcgatct cgcccgaaag catcgccccg gtggtgcggt cggtgttctt
     2761 gatcggcagg tcgatcttga ccggcttctt ctcctcgatt gcctcgcggg cgagcccgat
     2821 cagcttcaca tcaaggacct tgtcgaggcc gtggtcctgc cgctcggaat tgtagaccgc
     2881 gacctcgggc gggacttcgg gcttatggaa gatgcgggtg aaatcgaggc cgcgtgcctt
     2941 ccagtgatcg atcgcctggc ccttatcgag gatctccgtc tggccgatca tctcggagaa
     3001 tttgcggaag ccgagcgtgg ccatgtactc gcgcacctct tcggcgacga agaagaagaa
     3061 gttgatgacg tgctcgggct tgccctggaa gcgcgcccgc agcaccgggt cttgcgtggc
     3121 cacgccgacc gggcacgtat tcagatggca tttgcgcatc ataatgcagc ccgccgcgat
     3181 caagggcgcg gtggaaaagc cgaactcgtc ggcgccgagc aacgcgccca ccatcacgtc
     3241 gcggccggtg cggatgccgc cgtcgacttg gacggcgatg cgcccgcgca aacggttgag
     3301 gaccagcgtc tgctgggtct cggcgagccc gatttcccaa ggcgagccgg catgcttgat
     3361 cgaggtgagc ggcgaagcgc cggtgccgcc ctcgaagccc gcaatcgtca catggtcggc
     3421 catggctttg gcaacgcccg cggcgaccgt tccaaccccc acctcggcga cgagcttcac
     3481 cgagacatcg cccgtgggat tcacgttctt gaggtcgaag atgagctgct tgaggtcctc
     3541 gattgaatag atgtcgtgat gcggcggcgg cgagatcagt ccgacgcccg gcgtcgaatg
     3601 ccggaccttg gcgatggtgg cgtcgacctt atggccgggg agctgaccgc cttcgccggg
     3661 cttggctcct tgcgccatct tgatctgcat catgtcggca ttgatcagat attcggtggt
     3721 cacgccgaag cggcccgagg ccacctgctt gatcttcgag cgcatcgagt cgccgttcgg
     3781 cagcggccgg tagcggtccg attcctcgcc gccttcgccg gtgttcgact tgccgccgat
     3841 gcggttcatg gcaatggcaa gggtggtgtg cgcctcgcgg ctgatcgagc cgaacgacat
     3901 ggcgccggtc gagaagcgct tcacgatgtc cttggcgggc tcgacctcgt cgagggggat
     3961 ggggttccgg ccgatctcct cggcggtctt gatgcggaac atgccccgca acgtgaggag
     4021 ctgctcggtc tgctcgttga tctgcttggc gtaggagcga tacttcgccg gcaggctccc
     4081 gcgcacggcg tgctggaggt ccgcgaccac cgacggccgc cacatatgcg tttcgccgcg
     4141 cacgcgatag gcgtactcgc cgccgacgtc gagcgcctcg cggagcaccg gcgcggtcga
     4201 gaaggcgagc tcgtgacgct cgacggtttc ccgcgcgatt tcctccagac ccacgccctc
     4261 gacctggcta ttggtgccgg tgaaatattt ggcaacgaag tccgatctga gtccgacggc
     4321 atcgaagatc tgggcgccgc aataggactg ataggtggag atgcccatct tcgacatcac
     4381 cttcagtatg cccttgtcga tcgccttgat gtagcgcttg accgcttcct cggcggtaat
     4441 ctcctcgtcg agctcgggca gcatcgcctc gatggtctcg aaggcgagat agggattgat
     4501 cgcctcggcg ccgtagccgg caagggtgca gaattgatgc acctcatgcg cttcgcccgt
     4561 ctcgaccacg aggcccacgg aggtcctgag cccgcagcgg atcaggtgat ggtgcacggc
     4621 gctcgtggcg agcagcgagg ggatcggcac gcggtcggga cctgtggcgc ggtccgacag
     4681 gacgatgatg ttttcgccgg cacgaaccgc ctcctcggcg cggctgcaga gcgcaccgag
     4741 cgcctcggcc atgcccgtcg ccccgtgatc ggcgccatag gtgatgtcga gggtgacggt
     4801 acggaactgg ttgtcggcga cctcgccgat gccgcgaatg cgctcgaggt tctcgttggt
     4861 gagaatcgga tgcgacgcct cgagccgctt ctgtctggag gtgccttcga ggtcgagcag
     4921 gttcggccgc ggcccgatga aggtgacgag cgacatcacc agctcctcgc ggatcgggtc
     4981 gatcggcggg ttcgtcacct gggcgaaatt ctgcttgaaa taggtgtgca aaagcttggg
     5041 ccgcgacgac agcgccgaaa gcggcgtgtc ggtgcccatc gagcccaccg cctcctggcc
     5101 cgactcggcc atcggcatca tcaggaactt caggctttcc tgcgtgtagc cgaaggcctg
     5161 ttggcggtcg agcaggctga cattggtgcg cgggggcggg ggaggcgcgc ccggcagctc
     5221 atgcacgcga atctgggtgc ggtcgagcca ttgctgatag ggatgagctg atgcgagctc
     5281 cgccttgatc tcttcatcgg aaatgatgcg gcccttttcg aggtcgatga gcagcatctt
     5341 gccgggttgg agccgccatt tctcgacgat ggacttttcc gcgatcggca gaacgccggt
     5401 ctccgacgcc atgatgacga gcccctcgtc ggtgacgaga tagcgcgccg ggcgcagccc
     5461 gttgcgatcg agagtggcgc cgatctgtct gccgtcggtg aaggccatgg cggcgggacc
     5521 gtcccagggc tccatcagac aggcgtggta ttcgtaaaag gcgcggcgct tggcgtccat
     5581 gagcggattg ccggcccatg cctcggggat cagcatcatc gccgcatgcg ccaggctgta
     5641 tccgccctga accaggaact cgagcgcatt gtcgaagcag gcggtatcgg actgaccctc
     5701 gtaagaaatc ggccagagct tttcgatgtc gtcgccgaac aggggcgacg acacgctcgc
     5761 ctgccgcgcg gccatccagt tgaggttgcc tcgaagcgtg ttgatctcgc cgttatgggc
     5821 caccatgcgg taggggtggg cgagcttcca ggacgggaag gtgttggtcg agaagcgctg
     5881 atgcaccagc gcgagcgccg aggcgaagag cgggtgatgc aggtcgcggt aataggcgcc
     5941 gagctgatag gcgaggaaca tgcccttgta gacgagcgtg cggcatgaga gcgacacggt
     6001 gtagaagccg atctcccttc cccccgtctc gcccatgacg gtgttcgaga tcaccttcct
     6061 caggaggaag agcctgcgct cgaaagcggt ctcatcctcg atgccgggac cacggccgat
     6121 gaacacctgc cggtgcacag gttcggttgc gatcaccgac gcggaaaggc atgaattgtc
     6181 gcaaggaaca tcgcgccagc cgatgagggt gagcccttcc gcttcgatca cgcgctcgat
     6241 gacggactcg caataaatgc gctgggcctg attgcgcggc atgaacattt ggccgacgcc
     6301 gtaatggccg ggttcgggca gctcgatatt gagcggcgca caaaccgcac ggagaaaatc
     6361 atgcgggatc tgcacgag
//
LOCUS       DASUQP010000072         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_440077,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000072 DASUQP010000000
VERSION     DASUQP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440077"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFY74_03235"
     CDS             complement(<1..817)
                     /locus_tag="VFY74_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018116988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5930755.1"
                     /translation="MKRELLTVGLCLVVGAVFSPASIAQQVTGVPGAPSATSTIDGRQ
                     IPPPPGAFGGTINLDAQTSTPYWQPQVVPPKGAPNILLIITDDVGFAAPSTFGGVIPT
                     PTLDRIANMGVRYTQFHTTSLCSPTRAALITGRNHHSAGFGVISEAATGFPGYNSVIT
                     KDRSTIGQILKANGYATSWYGKNHNTPSYVTSQVGPFDQWPIGMGFEYFYGFNGGETN
                     QWQPDLYRNTTRIYPYAGNPNYNLTTDMADEAVAYLNQLNELDPKKPFFLLLRA"
     gene            complement(943..1809)
                     /locus_tag="VFY74_03240"
     CDS             complement(943..1809)
                     /locus_tag="VFY74_03240"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5930756.1"
                     /translation="MAKSRSDKIAFQGEPGANSHLAALDAYPRLEPLACPTFEDALAA
                     VKNGRAKLAMIPIENSVAGRVADIHHLLPHSRLHIVAEHFERVRHQLLTLPGVKLPEL
                     KTVHSHIMALGQCRGVIRALKLKPVAEADTAGAARHLRESGERSSAVIASKLAAKIYG
                     LKVARADIEDAEHNTTRFVVLATKPGRVKKSDGPVITSFVFRVRNVPAALYKALGGFA
                     TNGVTMTKLESYQLEGSFNATMFYADIEGHPDEPLVKLAMEDLNFYSSEVTVLGTYPA
                     SPFRAELKALAR"
     gene            complement(1835..2575)
                     /locus_tag="VFY74_03245"
     CDS             complement(1835..2575)
                     /locus_tag="VFY74_03245"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX5930757.1"
                     /translation="MTSCLIVIPARLKATRLPDKPLADIAGEPMIVHVWRRAVEANCG
                     SVVVATDAENVREAVAAAGGEALMTRKDHASGSDRVFEAVLRYDPGGKLETIVNLQGD
                     LPTLDPALVRACLRAKTEGGADIGTIAAEIVRHEERTDPNVVKVIGSPLRNGSALRAL
                     YFTRATAPYGDGPLYHHIGIYAYSRAALARFVSLPPSPLEQRERLEQLRALEAGMLIH
                     VGLVDTVPLGVDTPPDLERARELLRSRP"
BASE COUNT          471 a          765 c          900 g          472 t
ORIGIN      
        1 cggcgcgtaa tagaaggaag aacggcttct tgggatcgag ctcgttcagc tggttcaagt
       61 aggcgacagc ctcatcggcc atgtcggtgg tgaggttgta attcggattg ccggcgtaag
      121 gatagatgcg cgtggtgttg cggtagagat ccggttgcca ctgattggtc tcgccgccgt
      181 tgaagccata gaaatattcg aagcccatgc cgatcggcca ttggtcgaag gggccgacct
      241 ggctcgtcac ataagacggc gtgttgtggt tcttgccgta ccaggaggtc gcatagccgt
      301 tggccttgag gatctggccg atggtcgagc ggtccttggt gatcacgctg ttgtagccgg
      361 gaaagccggt cgcggcctcg ctgatcacgc cgaagccggc cgaatggtga ttgcgcccgg
      421 tgatgagggc cgcgcgcgtc ggggagcaga gcgaggtggt gtgaaactgc gtgtagcgca
      481 cgcccatatt ggcgatgcgg tcaagggtcg gcgtcggaat gacgccgccg aaggtgcttg
      541 gcgccgcgaa gcccacatcg tcggtgatga tgaggagaat gttgggagcg cctttcgggg
      601 gcactacttg gggctgccaa tagggcgtcg aggtctgcgc gtcgagattg atggtgccgc
      661 cgaaggcccc gggcggcggg ggaatctgcc gtccgtcgat cgtcgaggtg gcgctcggcg
      721 ctcccggaac accggtcacc tgttgggcga tggaagcggg cgagaaaacc gcgcccacga
      781 caagacagag accgaccgtc aacaactctc ttttcatttc tccctcccca aacgcgaccc
      841 ggttacgctc tgtctacgga atagcggaga tttttgcagc tgtcagcccc aggcgtccgc
      901 aggtgtcatg tcaggacatg acggcgcaaa aatcgacgcc catcatcgcg caagcgcctt
      961 caactcggcg cggaaggggc tcgccggata ggtgccgagc accgtcacct cgctcgagta
     1021 gaagttgagg tcctccatgg cgagcttgac cagcggctcg tcgggatgcc cctcgatatc
     1081 ggcatagaac atggtggcgt tgaacgagcc ttcgagctgg taggattcga gcttggtcat
     1141 ggtcacgccg ttggtggcga acccgccgag cgccttgtag agagccgccg gcacgttgcg
     1201 cacgcgaaac acgaagctgg taatgaccgg gccgtcgctc ttcttgacgc gcccgggctt
     1261 ggtggcgagc acgacgaagc gcgtggtgtt gtgctccgca tcctcgatat cggcacgtgc
     1321 cactttcaac ccgtaaatct tcgcggcgag tttcgaggcg atcaccgcag agctgcgctc
     1381 gccggattcg cggagatgcc gggctgcgcc ggcagtgtcg gcctcggcga caggtttcag
     1441 cttcagcgcg cggatgacac cgcggcattg gccgagcgcc atgatatggc tgtgcaccgt
     1501 tttcagctcc ggcagcttga cgccgggaag cgtgaggagc tgatggcgca cccgctcgaa
     1561 atgctcggcg acgatatgaa gacgcgaatg cggcagaagg tggtggatgt cggcgacgcg
     1621 ccccgccacc gaattctcga tcggaatcat ggcgagcttg gcccggccgt ttttcaccgc
     1681 ggcaagcgca tcctcgaagg ttggacaggc aagcggctcc aggcgcggat aggcgtcgag
     1741 cgcggcgaga tgcgagttgg cgccgggctc gccctggaag gcgattttgt ctgagcgcga
     1801 tttggccatg gtctgaggga agcgggcgaa ggctctaagg gcgggatcga agcagctcgc
     1861 gcgcccgctc aaggtcgggc ggagtatcga caccgagcgg gacagtgtca acgagaccga
     1921 catggatcag catgcctgct tccagcgccc gcagctgctc cagcctctct ctctgttcga
     1981 gcggcgacgg cggcaaggaa acgaagcgtg cgagcgccgc ccgcgaatag gcgtagatgc
     2041 cgatatggtg gtagagcggg ccatcgccgt aaggggcggt cgctcgcgtg aagtagagcg
     2101 cgcgcaacgc cgagccgttg cggagcggtg agccgatcac cttcaccacg ttcgggtcgg
     2161 tgcgctcctc gtggcggacg atctcggcgg cgatggttcc gatatcggcg ccgccctcgg
     2221 tcttggcgcg tagacaagcg cggacgagcg cgggatcgag ggtgggcaaa tcgccctgca
     2281 gattgacgat cgtctcgagc tttccccccg ggtcgtagcg gagaaccgcc tcgaagacgc
     2341 ggtcggaccc gctcgcatga tccttgcgcg tcatcaacgc ttcgccgccg gcggcggcaa
     2401 ccgcctcgcg aacattctcg gcgtcggttg caaccacgac cgagccacaa ttggcctcga
     2461 ccgcccgcct ccaaacgtgg acaatcatcg gctcgccggc gatgtccgcc aagggcttat
     2521 cgggcaaccg tgtcgctttc agccgcgcgg gtatgacgat gagacatgac gtcatgacat
     2581 gcgtcattag cacgaggaac ccggctcg
//
LOCUS       DASUQP010000073        13259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1393886, whole genome shotgun sequence.
ACCESSION   DASUQP010000073 DASUQP010000000
VERSION     DASUQP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393886"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFY74_03250"
     CDS             <1..276
                     /locus_tag="VFY74_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5930758.1"
                     /translation="WAYLVLGRFAEAVAALTQTEFNDAHLLLAIAYVRLGRLAEARAE
                     VGKMMKINPAITLQTWRQGYSFRDPAILERHTLDLVQSGLPETQRRS"
     gene            428..1810
                     /locus_tag="VFY74_03255"
     CDS             428..1810
                     /locus_tag="VFY74_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5930759.1"
                     /translation="MLNLKEDAGEQFRNAFRGNLYGPRDEGYEEARKPYNAMIDKHPG
                     LIARCADVADVICAVNLARDHHWTLAVRGGGHNVAGLATCDDGLVIDLSSMKGVRVDP
                     AKRTARVGGGCTWGEVDHATNIFGLSAPGGVVSTTGVGGLTLGGGIGHLSRRFGLSID
                     NLLEVDMVLANGEFIKASADENSDLFWAIRGGGGNFGIVTSFLFRLHPVSMVVAGPTL
                     WPLEMTADVMRFYDEFLNSAPDDLAGVFAFLKVPAGPPFPEALHRKTMCGVVWCYTGP
                     EGQAAALFDPIREFGPPALFGVQSMPFPVLQSAFDPLYPPGDQNYWRADFVNALSDDA
                     IALHVEHGSRLPTPQSGMHLYPIDGAVHRVGRADTAFSYRDARWAGVIFAVDPDPSKA
                     ELLRKWTIDYWEALHPYSAGGAYVNFMMEEGQSRVRATYRDNFERLSKIKNHYDPGNL
                     FGVNQNILPS"
     gene            2118..3374
                     /locus_tag="VFY74_03260"
     CDS             2118..3374
                     /locus_tag="VFY74_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX5930760.1"
                     /translation="MTPPELLKAMFQAAVDAALPSLCVPAHLPQRPKGRTIVIGAGKA
                     SGAMAKALEDAWTGPIEGLVVTRYGYRVPTERLEVVEAAHPVPDAAGREAAIRIFKLV
                     QGLTEDDLVLCLISGGGSALLALPPEGLTLEDKQAVNKALLKSGATISEMNTVRKHLS
                     AIKGGRLAAAAAPAKVVALMISDVPGDDPSIIASGPTVPDPSTNQDALAIIEKYKIDV
                     PPAVRERLRTADETPKPGDKRLARVENLMIATPQASLEAAASVAEKAGVKPVILGDSI
                     EGEARDVALVHAGIARQCALRGQPEKPPCVLISGGETTITLKGKGKGGRNTEFLLALA
                     IALDGMKKIHAIAGDTDGIDGSEDNAGALIDPDTLKRAAERGVNAKAMLADNDPWGFF
                     NALGDLLVTGPTLTNVNDFRAVLIEK"
     gene            complement(3377..3661)
                     /locus_tag="VFY74_03265"
     CDS             complement(3377..3661)
                     /locus_tag="VFY74_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930761.1"
                     /translation="MRDIRSDLQERAAMIGEHIKATQAQFETMLEQIKRDHEGKVRDF
                     RAELDAVNVLIGMEQRRHEGAGPAHAQGVPQPQAANAPRPLSGILMRRAG"
     gene            3913..4773
                     /locus_tag="VFY74_03270"
     CDS             3913..4773
                     /locus_tag="VFY74_03270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX5930762.1"
                     /translation="MEGPTLWGLAIAFAILFMAFRALELFRPRGRRLPILRKGLLTDG
                     AYWLFTPFVTKAITRVCVVGIAIPFALIAYGKFDRELILQGFGPLSRLPLWVQAVAIL
                     AIGDFVGYWMHRAFHGRRLWRFHAVHHSSVDLDWLSAVRVHPVNDAVMRIVGTLPVLA
                     LGFAPVAVAGIVPVLTLMAILVHANLDWDWGPFRAVIASPRFHRWHHTDEEQARDKNF
                     AGLLPLWDILFGTYYMPRAERPERFGTMTPVPGGLIGQMVFPFRRQASPVTSKSAALA
                     KPGVPRQAQV"
     gene            complement(4787..5590)
                     /locus_tag="VFY74_03275"
     CDS             complement(4787..5590)
                     /locus_tag="VFY74_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX5930763.1"
                     /translation="MMDWLRNVVQDRRTERFIMALIVLNAVTLGLETSASVMAKYGPV
                     LVVIDRIIIGIFVAEILARLAVQRASFFRDGWNIFDVIVVGIALAPATAAFSVLRALR
                     VLRLLRLITAVPALQRVVGGLIGALPGMGSIVLLIGLVFYVSAVMAVNLYGKQFPELF
                     GTLPTSLYTLFTIMTLEGWTNDVVNPIMERHPYAWLFFIPFIVITTFWVLNLFIGIIV
                     NAMQEEHAKAEAEMMHDETAPIIRELQELKTEIAALREEVGGRKRRSRR"
     gene            complement(5719..6222)
                     /locus_tag="VFY74_03280"
     CDS             complement(5719..6222)
                     /locus_tag="VFY74_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX5930764.1"
                     /translation="MPTFAKETTVMVGGAPMFPSKNIIQNAVNSKDHTTLVAAVKAAG
                     LVDTLSGKGPFTVFAPTNDAFAKLPAGTVDTLLKPENKGQLTQVLTYHVVPGRLSAKD
                     LMAAAEKGGGKAMLKTVEGEDLTVEIKDGAIWVWDAKGSTAKITIQNVMQSNGVIHVI
                     DTVLLPS"
     gene            complement(6519..7229)
                     /locus_tag="VFY74_03285"
     CDS             complement(6519..7229)
                     /locus_tag="VFY74_03285"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5930765.1"
                     /translation="MSDTGKKDSEGMGLTAAEYVLGVLNAAQRRAAERRIDRDPAFAR
                     EVAFWEERLGGLAQAVPEIAPPQRLWSRIEARLGRATNAEDERGGLWRSLSFWRGLGL
                     GASALAAASTAALIYVGAVGTRASPLIARLDAEGGQAGFVAAVNTGGGSLTIVPASLL
                     AAEQKAMELWLIPPGDKPHSLGLIDPNRPVTIKVPKDLLTRVNNEAVLAVSLEPPGGS
                     PTGQPTGPVIANGKLAQL"
     gene            complement(7226..7783)
                     /locus_tag="VFY74_03290"
     CDS             complement(7226..7783)
                     /locus_tag="VFY74_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5930766.1"
                     /translation="MDAEARRRQLAAALRRVAGGDRAALRHVYAETSAKLFGVCLRIL
                     NDRSEAEDVLQDVYLNVWRKARSFDEARASPITWLVAIARNRAIDRVRSGAGARSSAP
                     IDAASDVPDPTPLAAETIETAQENRRLYSCLEELETRQQAAIRAAYMDGLTYEELAER
                     SNVPLGTMKSWIRRGLAKLRACLER"
     gene            8134..9105
                     /locus_tag="VFY74_03295"
     CDS             8134..9105
                     /locus_tag="VFY74_03295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5930767.1"
                     /translation="MTLRRAGLGLIVGAVLMAPSAASAGPALVFEPFNGTVFYAEDPD
                     AAWFPASLTKLMTAYVAFQALKAGTVKLDSKVICSKKATEQAPSKLWLKEGEAITLEV
                     ALQALIVKSANDVAVMIAETVAGSEEAFVARMNEAAKRLNMTRTVFANPNGLPNESQV
                     TTARDLAKLARAIIIEFPEHADLFTAIQVQVGKKLLRTHNGLLVNYPGADGMKTGFIC
                     DSGFNVVASATREGRKLVAVILGEPSVASRRERAVDLLDNGFKRYFWKSLFGTSLDGL
                     AIQASLSGGPTHLRESVCGAGRPKSTRKKVVRKKKKKTRSTANSSAQ"
     gene            9102..9587
                     /locus_tag="VFY74_03300"
     CDS             9102..9587
                     /locus_tag="VFY74_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930768.1"
                     /translation="MSPFTRQSLILAAFALLAAPLTLAVAEAAEPPDNIVEALAEAAR
                     ACKDMDGKPNTDAVLRMEDLNGDGGEDWIADYAKMKCEGGINPLCSDAGCTIQIYLWD
                     GEASWDVAFEDLVRSYKFGTSGGKRMLYVTTSGIPCNKPVTETCKYNYRLDKDTVVPI
                     E"
     gene            complement(9602..9934)
                     /locus_tag="VFY74_03305"
     CDS             complement(9602..9934)
                     /locus_tag="VFY74_03305"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX5930769.1"
                     /translation="MFRDDPRAFATDRAALALAKRAIGEGIDMRLTPQQRAFIYMPFQ
                     HSEARDDQARSIELFTALGAPLNLDFARRHQAIIARFGRFPHRNSVLGRTSTAEERAF
                     LQEAGSSF"
     gene            complement(9984..10139)
                     /locus_tag="VFY74_03310"
                     /pseudo
     CDS             complement(9984..10139)
                     /locus_tag="VFY74_03310"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
     gene            complement(10156..10836)
                     /locus_tag="VFY74_03315"
     CDS             complement(10156..10836)
                     /locus_tag="VFY74_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5930770.1"
                     /translation="MSRRNERESAAPPAIYLVAATVAAIAGFGTVYLSFAPSDNGRSG
                     ETVPAPAVGPSATGEGRAASPLAGLNKGAMAPLVLRPEPIDLPILSFADKDGASRSLE
                     DFAGKVVLLNIWATWCVPCREEMPALDKLRSKLGGKDFAVVAVNIDKGGAEKAAAFLK
                     ETGATHLPLYTDPQGKIFATLKAVGMPTTLIIDRAGREIARLVGPADWASPEALAVIE
                     AAISAPRA"
     gene            10877..12274
                     /gene="argH"
                     /locus_tag="VFY74_03320"
     CDS             10877..12274
                     /gene="argH"
                     /locus_tag="VFY74_03320"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5930771.1"
                     /translation="MTNTMWGGRFAASPSEIMEEINASIDFDKRLWRHDIVASKAHVA
                     MLGATGIIEAKEADEIARGLDTIAAEIEAGTFPFSRSLEDIHLNVESRLGELIGPLAG
                     KLHTARSRNDQVATDFRLYVRDEIGAIDASLAALQLALAKKALQHAGALMPGFTHLQT
                     AQPITFGHHLLAYVEMLGRDRGRFADAAERLAECPLGAGALAGTSFPIDREATARALG
                     FARASANSLDAVSDRDFALETLAAAAIAAMHLSRLAEEIVNWASPQWGFVRVSDRFTS
                     GSSIMPQKRNPDAAELVRAKTGRIAGAFQALLMVMKGLPLAYAKDMQEDKEAVFDALD
                     ALKLGIAAMTGMVEDLEPDLRAMKRAASSGHATATDLADWLVLKLGLHFREAHHTAGK
                     IVALAESRGTDLKKLPLSELKSVEKRITEEVYATLDAGSSVKSRTSYGGTAPANVKRQ
                     AKRWIKRLEKDAETR"
     gene            12325..12510
                     /locus_tag="VFY74_03325"
     CDS             12325..12510
                     /locus_tag="VFY74_03325"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX5930772.1"
                     /translation="MGSALRVLLTLVLVLTIGLSACGRRGSLDRPKPNYPETQADPST
                     KMSKAPARRLWIDRLLE"
     gene            12526..>13259
                     /locus_tag="VFY74_03330"
     CDS             12526..>13259
                     /locus_tag="VFY74_03330"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5930773.1"
                     /translation="MHHFAYRRGVSHANVLHAEEVDLVDLAAAVGTPFYCYSTATLER
                     HYRVFADAFAGMQDALVCYSVKANSNLGVLATLARLGAGMDVVSEGELRRALAVKVPP
                     ERIVFSGVGKTREEMAFALDAGILAFNVESEPELTALSEIAAARGRTALIAFRVNPDV
                     DAKTHHKIATGKAENKFGVPFEEAPSLYALASRLPGVAPTGVHMHIGSQITELAPFRN
                     AFALLRELVGSLRQSGFAIEFVNLGGG"
BASE COUNT         2386 a         4195 c         4255 g         2423 t
ORIGIN      
        1 tgggcgtatt tggtactggg gagatttgca gaggcggtgg ctgctctgac gcaaaccgaa
       61 ttcaacgacg cccacctgct cttggcaatc gcctacgtgc gccttggccg cttggccgag
      121 gcccgtgcag aagtggggaa aatgatgaag atcaatcctg cgatcactct gcaaacgtgg
      181 cggcagggat attccttccg cgaccctgca atcctcgagc gccacaccct tgatctcgtg
      241 caatcaggct tgccggagac acagcgccgt tcgtaggtga aaccgtaaca tactgggact
      301 gatcggcagg gactggcatg cttaggctgc gggtcatggc gtggtgagct gttccttcag
      361 ggatgctccc aagtctcgct ttagatggcg gtccatgaag ccagaatcga acctccgggg
      421 taacgccatg ctgaatctca aggaagacgc gggcgagcag ttccgtaatg cttttcgagg
      481 aaatctctac ggcccaagag atgagggcta tgaagaggct cgaaaaccct acaacgcgat
      541 gatcgataag cacccagggc tcattgcgcg atgtgccgat gttgccgacg tcatctgcgc
      601 ggtaaacctt gcacgagatc accattggac tctggctgtg cgcgggggag gccataatgt
      661 cgctgggctt gcaacgtgtg atgacggcct cgtaatcgat ctgtcgtcga tgaagggggt
      721 acgcgtggat ccggcgaagc gaactgcacg tgttgggggc ggttgcacat ggggagaggt
      781 cgatcacgcc accaatatct ttgggctgtc tgcgccaggt ggagttgttt ccacaacggg
      841 tgtcggcgga ctgactttgg ggggagggat tggacatcta agccgcagat tcggcctctc
      901 gatcgacaat cttctcgaag tcgacatggt tctcgcgaat ggagaattca tcaaggcgag
      961 cgccgacgaa aatagtgatc tgttctgggc aatccgcggt gggggcggaa actttgggat
     1021 cgtcacctcc tttctgttcc gacttcatcc ggtcagcatg gttgttgctg gacccactct
     1081 ttggccgctt gagatgacgg ccgacgtcat gcgtttctat gacgagttcc tgaacagcgc
     1141 gccagacgat ttggcaggcg tattcgcgtt cctaaaggtg ccagccgggc caccctttcc
     1201 cgaagccctg catcggaaaa cgatgtgcgg tgtggtgtgg tgctacacgg gcccggaagg
     1261 gcaggccgca gccctctttg accccatccg tgaattcggt ccgccagcct tgtttggcgt
     1321 gcagtcgatg ccattccctg tgctccagag cgcgtttgat ccgctctacc cgccgggtga
     1381 tcaaaattac tggcgagccg attttgtcaa cgctctcagc gatgacgcga tcgctctgca
     1441 cgtggagcat ggctcccggc ttcccacgcc tcagtccggg atgcatcttt atcccatcga
     1501 tggggcggta catcgtgttg gaagggccga cacggccttc agctatcgcg acgcgcgctg
     1561 ggcgggagtg atctttgcgg tggaccccga tcccagcaaa gctgagctac tgagaaagtg
     1621 gacgatcgac tactgggaag ccctccaccc gtattctgcg ggaggtgcct acgtcaactt
     1681 catgatggaa gagggacaat cccgggtccg tgccacgtat cgtgacaact ttgaacgcct
     1741 ttcaaagatc aaaaaccact acgatcccgg caatctattt ggagtcaatc aaaacatcct
     1801 accgagttga tagctttcat gacctaacgc gctcaacggt gggccggttg tagccaagta
     1861 ggagcgccca aactcccgtt acgggggcaa tgccgcaaaa aagaaagttg accacagata
     1921 ccacgtcgag ttggcgtcca tgactccctg tttagcagaa ccgcgaagcg cgggtgccaa
     1981 tcgggtgcca gtttcacact ttgtttgacg aagactaggg gtttcccaag gagcctcgcg
     2041 ccaagaaatt cattcgcttg ccttcttgca atcgtctgtg cagggtcatc ggccgaaatg
     2101 ggagggcatc tcgacatatg accccgccag aactcctcaa ggccatgttc caagccgcgg
     2161 tcgacgcggc gcttccctcg ctttgcgtgc cggcccatct gccgcaaagg cccaaagggc
     2221 ggacgatcgt gatcggcgcc ggcaaagcct ccggcgcgat ggccaaggcg ctggaggatg
     2281 cctggacggg tccgatcgag gggctcgtcg tgacgcgata cggctatcgc gtgccgaccg
     2341 agcggcttga agtggtcgag gccgcccatc ccgtgcccga tgccgcgggc cgcgaggccg
     2401 caatccgcat tttcaagctc gtccaggggc tgaccgagga cgatcttgtg ctctgcttga
     2461 tctcgggcgg cggctcggcg ctgctcgcgc ttccccccga gggcttgacg ctcgaggaca
     2521 agcaggcggt gaacaaggcc ctgctcaaga gcggcgccac gatctcggag atgaacacgg
     2581 tgagaaagca tctctcggcc atcaagggcg gcaggctcgc cgcggcggcg gcccccgcca
     2641 aggtcgtcgc gctcatgatc tccgacgtgc cgggcgacga tccgtccatc atcgcttcgg
     2701 gcccgaccgt gccggacccc tccaccaacc aagacgcgct cgccatcatc gagaagtaca
     2761 agatcgacgt gcctccggcc gtgcgcgagc ggctcagaac ggccgacgag acgccgaagc
     2821 caggcgacaa gcgcctcgcc cgcgtcgaga acctgatgat cgccacgccg caagcctcgc
     2881 tcgaggccgc ggcttccgtg gcggagaagg cgggcgtcaa gccagtcatc ctcggcgact
     2941 cgatcgaagg cgaggcgcgc gacgtggcgc tcgttcatgc cgggatcgcg cggcaatgcg
     3001 cgctgcgcgg ccagcccgag aagccgcctt gcgtgctcat ctcgggcggc gaaacgacca
     3061 tcacgctgaa aggcaaaggc aaaggcggcc gcaacaccga gttcctgctc gcgctcgcca
     3121 tcgcgctcga cggcatgaag aagattcatg ccatcgccgg agacaccgac gggatcgacg
     3181 gctcggagga caatgccggc gccttgatcg atcccgatac gctcaagcgc gccgcggaaa
     3241 ggggcgtgaa cgccaaggcc atgctcgccg acaacgatcc gtggggtttc ttcaacgccc
     3301 tcggcgatct gctcgtgacc ggccccacgc tgaccaatgt caacgacttc cgcgcggtct
     3361 tgatcgagaa gtagcgctag cccgcgcggc gcatcaggat gcctgacagc gggcgcggcg
     3421 cgtttgctgc ttgcggttgc ggcacgcctt gcgcgtgcgc gggccctgcg ccttcgtggc
     3481 gacgctgctc catcccgatc aggacgttga ccgcgtccag ctcggcgcga aaatccctga
     3541 ccttcccctc atggtcccgt ttgatctgct ccagcatcgt ttcgaactga gcctgcgtcg
     3601 ccttgatgtg ctcgccgatc atggccgccc gctcttgcag gtcgctgcga atatcgcgca
     3661 tggatggtcc cccgtttggt taatgaggct caacccagat atggccgtgc cggttccccg
     3721 ccaacgcgcc tgacaagcgt gagtgtgaca ttctggccgc ttggccggtt agcgccggcc
     3781 ccaaccggag ggcacgggcg ccttggccgc tccttgaagc ggcaagtttg aagcgcccga
     3841 gcgtcatgaa ttcggcctaa accttgccac attctgttac cgctcgtccg aacggcacga
     3901 cgggagccac tcatggaagg cccgaccctt tgggggcttg ccattgcctt cgccattctg
     3961 ttcatggcct ttcgcgcgct cgaactgttc cggccgcgcg gccggcggct gccgatctta
     4021 cggaaaggtc tcctgaccga cggcgcttat tggctgttca cgccgttcgt caccaaggcc
     4081 atcacccgcg tgtgtgtggt tggcatcgcc atacccttcg ccctcatcgc ctacggcaag
     4141 ttcgatcgcg agctgatcct gcaagggttc ggtcctcttt cacggctgcc gctttgggtc
     4201 caggccgtcg ccattttggc gatcggcgat ttcgtcggct attggatgca tcgcgccttt
     4261 cacgggcgca ggctctggcg cttccatgcc gtccaccatt cttcggtcga tctcgattgg
     4321 ctgtcggccg tgcgcgttca tccggtcaac gatgccgtca tgcggattgt ggggacgctg
     4381 ccggtgctgg cactcggttt tgcgccggtg gcggtcgccg gaatcgtgcc ggtgctgacg
     4441 ctcatggcca ttctcgttca cgccaatctc gattgggact gggggccatt ccgcgcggtg
     4501 atcgcgtcgc cgcgctttca tcgctggcat cacaccgatg aagaacaggc gcgcgacaag
     4561 aatttcgccg gtctcctgcc gctgtgggac attctcttcg gcacctacta catgccgcgg
     4621 gccgagcggc cggagagatt cggcaccatg actcccgtgc cgggcggcct gatcgggcag
     4681 atggtgtttc cctttcgccg gcaggcttcg cccgtgacgt ccaaatccgc cgctttggcc
     4741 aagcctggcg tgccgcgcca ggcgcaggtc tgacgacctc cgcggctcag cgccgggagc
     4801 gccgcttgcg gccgcccact tcctcgcgca gcgccgcaat ctcggtcttc aattcctgaa
     4861 gctcacggat gatgggcgcc gtctcgtcgt gcatcatctc ggcttcggcc ttggcgtgct
     4921 cttcctgcat ggcattgacg atgatgccga tgaagaggtt cagcacccag aaagtggtga
     4981 tgacgatgaa gggaatgaag aacagccagg cgtaggggtg cctttccatg atcgggttga
     5041 cgacgtcgtt ggtccagcct tccagcgtca tgatggtgaa cagcgtgtag agcgaggtgg
     5101 gaagcgtgcc gaacagctcc ggaaattgct tgccgtagag attcacggcc atcaccgcgc
     5161 tgacatagaa gacaagcccg atcaggagca cgatcgagcc catgcccggc agagcgccga
     5221 tgagaccgcc cacgacacgc tgcaacgccg gaaccgccgt gatcagtctc aggagacgca
     5281 acacgcggag cgcgcgcagc accgagaacg ccgcggtcgc gggcgcgagc gcgatgccga
     5341 cgacaatgac gtcgaagatg ttccatccgt cgcgaaagaa gcttgcgcgt tgcaccgcga
     5401 ggcgggcgag tatttcggcg acgaagatgc cgatgatgat acggtcgatg acgacgagca
     5461 ccggaccgta tttcgccatc acgcttgcgc tggtctcgag cccgagggtc acggcgttga
     5521 gcacgatgag cgccatgatg aagcgctcgg tccgccggtc ctggaccacg ttacgcagcc
     5581 aatccatcat cgaagcgcct cgggctccct ggaatttccg ccccgatcta agcgttatcc
     5641 cgttctgaga caaggaaagc ccgccgcgcg ggagcacggc gggcttggct gatgcgcaag
     5701 cttggggggg tcgagtcttt agctcggcaa cagcacggta tcgatgacgt ggatgacgcc
     5761 gttcgactgc atgacgttct ggatggtgat cttggcggtg ctgccttttg catcccacac
     5821 ccagatcgcg ccgtccttga tttcgacggt gaggtcttca ccttcgacgg tcttgagcat
     5881 ggccttgccg ccgcccttct cggccgccgc catcaggtcc ttggcgctga gacgtccggg
     5941 aacgacgtgg taggtcagga cctgcgtcag ctggcccttg ttctcgggct tgagcagggt
     6001 gtcgaccgtg ccggccggga gcttggcgaa ggcgtcgttg gtcggcgcga acaccgtgaa
     6061 cgggcccttg cccgacagcg tgtcgacgag accggcggcc ttgacggcgg caaccagcgt
     6121 ggtgtggtcc ttcgagttga cggcgttctg gatgatgttc ttcgagggga acatcggggc
     6181 gccgcccacc atcaccgtcg tctccttggc gaaggtcggc atggcggcaa aagctgcgat
     6241 agcaagaccg gcggcagcaa caccggacag agcgaatttg gcgaagttca taacatcctc
     6301 ctgtatttgc gggttgtccc ttgggggggt acgggcggag ttacggagga ggcaggagct
     6361 gcggatgcag cggccgcgat gacgttttcg tgagcggccg ctctcgacgt catgcccgcg
     6421 caggcgggca ccagaccccc ggcgatcggg accagcgagc ggcggcggcc actggatcgt
     6481 ccgccttcgc ggacgatgac aagggcaaag tgctcgagct acagctgagc cagcttgccg
     6541 ttggcgatca cggggccggt gggctggccg gtgggcgagc cgccgggcgg ctcgagcgag
     6601 accgcaagca cggcctcgtt gttgacgcgg gtcaggaggt ccttcggcac cttgatcgtc
     6661 accggcctgt tcggatcgat cagccccagc gaatgcggct tgtcgccggg cgggatcagc
     6721 caaagctcca tggctttctg ctcggcggcc agcagcgagg ccggaacgat ggtgaggctg
     6781 cccccgccgg tgttgacggc ggcgacgaag cccgcctgcc cgccttcggc gtcgagcctg
     6841 gcaataagag gcgaagcccg tgttccgaca gcgccgacat agatcagagc ggcggttgag
     6901 gccgcggcaa gcgcgctcgc gccaagccca aggccgcgcc agaaactcaa gctccgccac
     6961 agcccgccac gctcgtcctc ggcatttgtg gcgcggccga gtcgagcctc gatcctcgac
     7021 cacaggcgtt gcggcggcgc gatctcgggg acggcttgcg caagtccgcc gagccgctct
     7081 tcccaaaagg cgacctcgcg cgcgaaggca gggtcgcggt cgatccttcg ctcggcagca
     7141 cgccgctgcg cggcgttgag cacgccgagc acatactcgg ccgcggtcag gcccatgcct
     7201 tcagagtctt tcttgccggt gtcgctcatc gctcaagaca cgctctcaac ttggcaagcc
     7261 cacgccggat ccagctcttc atcgtgccga gcggcacgtt tgagcgttcg gcgagttcct
     7321 cataggtcag tccgtccatg taggccgcgc gaatcgccgc ctgctgccgc gtctcgagtt
     7381 cctcgaggca ggaatagagg cggcggttct cctgggcggt ttcgatcgtc tcggcggcaa
     7441 gcggcgtggg gtccggaacg tcggaggccg catcgatcgg cgcgctcgat cgcgcgcccg
     7501 cgcccgaacg cacgcggtcg atggcgcgat tgcgcgcaat ggcgacgagc caggtgatcg
     7561 ggctcgcgcg cgcttcatcg aagcttcggg cctttcgcca tacgttgaga taaacgtcct
     7621 gtaacacgtc ctctgcctcg ctgcggtcat tcaagatacg gaggcagacg ccaaaaagct
     7681 tcgcagaggt ctcggcatag acgtggcgca aggcggcgcg gtcgccgccg gcgacccggc
     7741 ggagcgccgc ggcgagctgc cgtcgccttg cctctgcatc catgacgctg agcccgaacc
     7801 ggttgcaacg cgcccctctc ctaccgcgca ggaacccatt tgtctctaac cgccgggggc
     7861 ggaatttcgc agttgcgaat tagggggacc gcgttactct ccccggcgag accagggttc
     7921 gctaagcttt tcgcgattcg ggggtgacgg cgcgaggggc tttgcatccg tgggcgtcag
     7981 gtgggatgcg gtccggtcca gcgccacgga acagtcgaaa tttgaaccac tatgctatgg
     8041 caaacggcct agcgggaggg ccgcgagatc gtcgtctgag gcaagctttg tcctggtgct
     8101 gcgcggcggg cgagatggga tgaggaattg gggatgacgc tgcggcgtgc cggtcttggg
     8161 ctcatcgtcg gtgccgtgtt gatggcgccg agcgccgcct cggctggtcc cgcccttgtg
     8221 ttcgagccct ttaacggcac ggtattctac gccgaagacc ccgacgccgc ctggtttccc
     8281 gcctctctca ccaagctgat gacggcctat gtcgcctttc aggcgctgaa agccggcacc
     8341 gtcaagctcg acagcaaggt catctgctcc aagaaggcga cggagcaagc accgagtaaa
     8401 ctctggttga aagagggcga ggcgatcacg cttgaggtcg cgctccaagc cctgatcgtc
     8461 aaatcggcca atgacgtcgc ggtcatgatt gccgaaacgg tcgcgggcag cgaggaagcg
     8521 ttcgtggcgc gcatgaacga ggcggccaag cgactcaaca tgacgcgcac cgttttcgcc
     8581 aatccaaacg gacttccaaa cgagagccag gtcacaaccg ctcgagacct cgccaaactc
     8641 gcccgcgcca tcatcatcga atttcccgag catgccgatc tgttcacggc catccaagtc
     8701 caggtgggca agaagctcct gcgcacgcat aacggcctcc tcgtcaacta tccgggagcc
     8761 gacggcatga agaccgggtt catctgcgac tcaggcttca acgtggtcgc gagcgcgacc
     8821 cgcgaaggcc gcaagctcgt ggcggtcatt ctcggcgagc cgtcggtcgc ctcgcgccgc
     8881 gagcgtgcgg tcgatctgct cgataacgga ttcaagcgct atttctggaa atcgctgttc
     8941 ggcacgagtc tcgacggact cgccattcag gcctcgctga gcggcggccc cacgcatctt
     9001 cgcgaaagtg tctgcggcgc gggcagaccc aaatcaacgc ggaaaaaggt ggtccgcaag
     9061 aagaagaaaa agacgcggag caccgcaaac tctagcgctc agtgagcccg ttcacccgcc
     9121 aatcgctcat ccttgccgcg ttcgccctcc tcgccgcgcc tttgacgctc gcggtcgccg
     9181 aggcggccga gccgccagac aatatcgtcg aggctctcgc cgaggcggcg cgcgcctgca
     9241 aggacatgga cggcaagccg aacaccgatg ccgtgctccg catggaggat ttgaacggcg
     9301 acggcggcga ggactggatc gccgattacg ccaagatgaa atgcgagggc ggcatcaatc
     9361 cgctctgcag cgacgccggt tgcacgattc agatttatct gtgggacggc gaggcctcat
     9421 gggacgtcgc gttcgaggat ctcgtgcgga gctacaagtt cggcacgagc ggcggcaagc
     9481 gcatgctcta tgtgacgacc tcaggcatcc cctgcaacaa gccggtcacc gagacctgca
     9541 aatacaatta tcgcctcgac aaggacacgg tggtcccgat cgagtagagc ggaatcgtcg
     9601 gctagaagga cgagcccgcc tcctgcaaga aggcgcgttc ctctgcggtc gaggttcggc
     9661 cgagcacgct gttgcgatgg ggaaagcggc cgaagcgcgc gatgatcgcc tggtgcctcc
     9721 gggcaaagtc gagattcagc ggcgcgccga gcgccgtgaa cagctcgata gagcgtgctt
     9781 gatcgtcgcg cgcctccgaa tgctggaacg gcatgtagat gaaggcgcgc tgctggggcg
     9841 tgagccgcat atcgataccc tcgccgatcg cgcgcttggc aagcgcaagt gcggcccgat
     9901 ccgtggcgaa agcgcgcgga tcgtcgcgaa acatgttccg tgggaaggag gatgggggcg
     9961 agcatgccct tcggctcggc gatccactcg ggcggcacgc tatccttcag tgcctcatac
    10021 gtcgcaccga aacgtcggct gatctcggca tcgatcgccg gatccttgcg ataccagtcc
    10081 tcgggcttca gcgtctcgaa ccaaaaggag acaatctcgt cgatgatgcc ggcagtcatc
    10141 ggcgcttccc catcgctagg cgcgcggcgc ggagatcgcc gcctcgatga ccgcgagcgc
    10201 ttcgggcgag gcccagtcgg ccggaccgac gaggcgagcg atctcgcggc ccgcgcgatc
    10261 gatgatgagc gtggtcggca tgccgaccgc cttcagcgtg gcgaagattt tcccttgagg
    10321 gtcggtgtag aggggaagat gcgtggcgcc ggtctctttt agaaaggcgg ctgccttttc
    10381 cgcaccccct ttgtcgatat tgaccgcaac cacggcgaag tccttgccgc cgagcttcga
    10441 tcggagcttg tcgagcgcgg gcatctcctc gcggcagggc acgcaccacg tcgcccagat
    10501 gttcagcagc acgaccttcc ctgcgaagtc ttcgagcgac ctcgacgcgc cgtctttgtc
    10561 ggcgaaggaa aggataggta aatcaatcgg ttctgggcga aggacgagcg gcgccatggc
    10621 gcccttgttg agcccggcga gcggacttgc cgcgcgccct tcgcccgtcg ccgaggggcc
    10681 caccgcgggc gccgggaccg tctcgccgga gcggccattg tcagaggggg cgaaactcaa
    10741 atataccgtg ccgaatccgg cgatcgccgc aactgttgcg gcgacgagat agatggccgg
    10801 cggcgcggcg ctttcgcgtt cgttgcgtct gctcaaatct tcccgctcac ctcgtctggc
    10861 cctaggcgcg acagcgatga cgaataccat gtggggcggc cgctttgccg ccagtccttc
    10921 cgaaatcatg gaggagataa atgcctccat cgatttcgac aagcgcctgt ggcgtcacga
    10981 tatcgtggcg tcgaaggcgc atgtcgccat gctgggcgca accggcatca tcgaggcgaa
    11041 agaggccgat gagatcgcgc ggggtctcga cacgatcgcc gccgagatcg aggcgggcac
    11101 cttccccttc tcgcgttcgc tcgaggacat tcatctgaat gtcgagagcc ggttggggga
    11161 gctgatcggg cctctcgccg gcaagctcca tacggcgcgc tcgcgcaacg accaggtggc
    11221 gaccgatttc aggctctatg tgcgggacga aatcggagcg atcgacgcca gcctcgccgc
    11281 gctccagctc gcgctggcaa agaaggcgct gcaacatgcg ggcgcgctga tgccgggctt
    11341 tacccatctc cagactgcgc agccgatcac gttcggccac caccttctcg cttatgtcga
    11401 gatgctcggg cgggaccggg gtcgcttcgc cgacgccgca gagaggctcg ccgagtgtcc
    11461 cttgggagca ggcgcgctcg ccggcacctc ttttccgatc gaccgggagg cgacggcacg
    11521 cgcgctcggc tttgcgcggg cctccgcgaa ctcgctcgat gcggtctccg accgcgactt
    11581 cgcgctcgag acgttggcgg ctgccgccat cgccgccatg catctgtcgc ggctcgccga
    11641 ggagatcgtg aactgggcct cgccgcagtg gggcttcgtg cgtgtctccg acagattcac
    11701 gtccggttcg tcgattatgc cgcagaagcg taaccccgac gccgccgagc tggtgcgcgc
    11761 aaagaccggg cgcatcgcag gcgcgttcca ggcgcttctc atggtgatga agggcctgcc
    11821 tctcgcctat gccaaggaca tgcaggagga caaggaggcc gtgttcgatg cgctcgacgc
    11881 gctgaagctc gggatcgccg ccatgaccgg catggtcgag gacctcgagc cggacctcag
    11941 ggccatgaag cgcgccgcgt cctcgggcca tgcgaccgcg accgaccttg ccgattggct
    12001 tgtgctcaag ctcgggcttc acttcaggga ggcccaccac accgccggca agatcgtggc
    12061 gcttgccgaa tcacggggca ccgacctcaa aaaactgccg ctttccgagc taaaatcggt
    12121 cgaaaagcgg atcaccgagg aggtttacgc gacgctcgat gccggaagct cggtcaaaag
    12181 ccgcactagc tatgggggaa ccgcccccgc caacgtcaaa aggcaggcga aacggtggat
    12241 aaagcggctg gagaaggacg cggaaacacg ataaaaggat agtcgaagag ccgccaaaag
    12301 gcttgaggga acaggggcac gaccatggga agcgcgctgc gcgtcctttt aacgcttgtg
    12361 ctggtgctga caatcgggct ttcggcctgt ggacgccgag gctcgttgga ccggcccaag
    12421 cccaactatc ctgaaaccca ggccgacccg tccaccaaga tgtcgaaagc cccggcccgc
    12481 cgcctttgga tcgaccgcct gctcgaatag acccgccttt ctccgatgca ccatttcgcc
    12541 taccggcgcg gcgtgtccca cgcaaatgtg ctccacgccg aggaggtcga tcttgtcgat
    12601 ctcgccgccg cggtaggaac gcccttctat tgttattcga cggcgacgct cgagcgtcat
    12661 taccgcgtct tcgccgatgc cttcgctggc atgcaagacg cgctggtctg ctattcggtc
    12721 aaggccaact ccaatctcgg cgttctcgcc acgctcgccc ggctcggcgc aggcatggac
    12781 gtcgtgtcgg aaggcgagct tcgccgcgcg ctcgcggtca aggttccgcc cgagcggatc
    12841 gtgttctcgg gcgtgggcaa gacgcgcgag gagatggcgt tcgcgctcga tgccggcatc
    12901 ctcgccttca acgtcgagtc ggaacctgag ctcacggcgc tcagcgaaat cgccgcagcg
    12961 cgagggcgca ccgcgcttat cgcctttcgc gtaaaccccg acgtcgacgc caagacccac
    13021 cacaagatcg cgaccggaaa ggccgagaac aaattcggcg tgcctttcga ggaagcgcct
    13081 tcgctctacg cgcttgcgag ccggcttccc ggcgtcgcgc ccaccggcgt gcacatgcat
    13141 atcggcagcc agatcacgga gcttgcgccg ttccggaacg cgtttgccct gctgcgggag
    13201 ctcgtcggaa gtttgcgcca aagcggcttc gccatcgagt tcgtcaatct cggcggcgg
//
LOCUS       DASUQP010000074         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1667771, whole genome shotgun sequence.
ACCESSION   DASUQP010000074 DASUQP010000000
VERSION     DASUQP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667771"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1149)
                     /locus_tag="VFY74_03335"
     CDS             complement(184..1149)
                     /locus_tag="VFY74_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5930774.1"
                     /translation="MRKALLALLLLGVAGGLAFYLLTMPVRMPASALPAHMPDLAYGK
                     YMFTAGGCAECHAVPLEDCDDLKIKDAEILAGGRCLKTPFGTFNVPNISPDKDTGIGH
                     WTTADFVNAMKHGIAPNGTYLYPAFPYPSYQRMTYEDLIDLKAYLDSLPAVKSSVPPS
                     ELRFPYNVRRALGLWNRLYVDGKSFMPEPGASAEINRGAYLVRGPAHCSECHSSRNLL
                     GGIVKDTEFAGAPNLESDGSVPNITPSKDGIGDWSEEDIAYLLETGNTPDYDTIGETM
                     APVQKNMAKLTPEDRNAIAAYIKSLPPRPDAVPKSKKPADGEDRD"
     gene            complement(1193..1648)
                     /locus_tag="VFY74_03340"
     CDS             complement(1193..1648)
                     /locus_tag="VFY74_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX5930775.1"
                     /translation="MNSHAVRSIKLGAMFAAALAIGQASAPSPASAQVIERGIQGGVA
                     GAIIGGIIDGGKGAGRGAAIGAGIGVVTGAIERDNAQRAYAYGPAPYDLVYDTQVALY
                     ELGYDPGPPDGVYGRRTADAIAQYEYAARLPVTGRPSQALLEHMHRYGG"
     gene            complement(1780..2409)
                     /locus_tag="VFY74_03345"
     CDS             complement(1780..2409)
                     /locus_tag="VFY74_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5930776.1"
                     /translation="MIELYTWTTPNGWKASIMLEEVGLPYRVHAIDITKGDQFQPSFL
                     KIAPNNKIPAIVDTDNGLHLMESGAILLYLAEKTGKLWPQDFPAKWRVVEWLMWQMGG
                     PGPFLGQVHHFVKFNPGKSSYAEERYAKEAKRLWGVLDRRLGEVEYVAGDYSIADIAI
                     WPWISRFAWQTVDFADHPHVKRWYLTIAERPAVKRGWDVLKEGRAIPLP"
     gene            2700..>3293
                     /locus_tag="VFY74_03350"
     CDS             2700..>3293
                     /locus_tag="VFY74_03350"
                     /inference="COORDINATES: protein motif:HMM:NF018053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HEX5930777.1"
                     /translation="MPNSAHRDRQSPHAASSQTSFARYAILFTPRPGTALAAFGRSWF
                     GRANDGVTLQAFSDAGLSAASFARLAPMPGRYTGLHALIKAPFAPRAGIGLDAIKTRL
                     INFARRRNLLATGPLTLSRDGRFLVLRPVEATPGLDWLAAQCVASLEDFESSPAEAES
                     QYRFSITLTGPLEKAHLERVTQALWPVLEEICASGVTV"
BASE COUNT          575 a         1091 c         1009 g          618 t
ORIGIN      
        1 agcgagcgcg gtgaggccca aagccgcaac gacgagcccg aggatcaggc cccttcccgc
       61 tcccgatttc cccgccattg ggtttcccta atgcttcgcc gcaaccggct tcgccgcaag
      121 cgcattttgc cgcgcgccga atactagcac cattctcgcc ccgccgggac tgctgctgtc
      181 tcctcaatca cggtcctctc cgtccgccgg ttttttcgac tttggcacgg catcgggccg
      241 cggcggcagc gacttgatat aggcggcgat ggcgttccgg tcctctggcg tcagcttggc
      301 catgttcttc tgcaccggcg ccatagtctc gccgatcgta tcgtagtcag gcgtgtttcc
      361 cgtctcgagc agataggcga tgtcttcttc gctccagtcg ccgatgccgt ccttgctcgg
      421 cgtaatattg ggcacagagc cgtcgctctc gaggttcggc gccccggcga attcggtgtc
      481 cttgacgatg ccgcccaaga gattgcgcga agagtggcat tccgaacaat gcgcggggcc
      541 ccgcaccaga taggcgccgc ggttgatttc ggcgctcgcg ccaggctcgg gcatgaagct
      601 cttgccgtcg acatagagcc ggttccagag gccgagcgcg cggcggacat tatacgggaa
      661 gcggagttcc gacggcggca cgctgctctt caccgcggga aggctgtcga gataggcctt
      721 gaggtcgatc aggtcctcgt aggtcatgcg ctggtatgac ggatagggaa aggccggata
      781 gagataggtg ccgttaggcg cgatcccatg tttcatggcg ttaacgaagt cagccgtggt
      841 ccaatggccg atgccggtgt ccttgtcggg agaaatgttg ggcacgttga aggtgccgaa
      901 cggcgtcttc aggcagcggc cccctgcgag gatctcggca tccttgatct tgagatcgtc
      961 gcagtcctcg agcgggacgg cgtgacactc cgcgcagccg ccggcggtga acatgtactt
     1021 gccgtaggcg agatcgggca tgtgcgcggg gagcgccgat gccggcatcc tgaccggcat
     1081 ggtcaagagg tagaaggcaa ggccgccggc aacgccgagc aagagcaaag ccagcagcgc
     1141 cttacgcaac gctccactcc cgcagaccta acgtttcgtg cggcgccgag cctcagccgc
     1201 cataccggtg catgtgttcg agcaaggctt gcgacggacg ccccgtgacc ggcaggcgag
     1261 ccgcatattc atattgggcg atcgcgtcgg cggtacgccg gccataaacg ccatcggggg
     1321 gacccggatc ataaccgagt tcataaagag cgacctgcgt gtcgtagacg aggtcgtacg
     1381 gtgcggggcc ataagcatag gcacgctgcg cgttgtccct ctctatggcg ccggtcacga
     1441 ccccgatccc ggccccgatc gccgccccgc gacctgctcc cttgcccccg tcgataatgc
     1501 ctccgatgat cgcgcccgca acgccgccct ggattccgcg ctcgatcact tgcgcggaag
     1561 cggggctcgg cgcggacgcc tgaccgatcg ccagcgcggc agcaaacatc gcgccgagct
     1621 tgatcgagcg cacggcatgg ctgttcatgt tgggtctcct ctcgcatcag gcctcaaacg
     1681 gcgagcgctt taccccaacc cccgtcgggc gctcgccgcg gctcaagcgt catcctgcca
     1741 cagccgccgc gcaagccgcc agacagcttg accggaagtt catggcaggg gaatggcgcg
     1801 gccctctttc agcacatccc agccacgctt gaccgcaggc cgttcggcga tcgtcaaata
     1861 ccagcgcttc acatgcggat ggtcggcaaa atccaccgtt tgccaggcga aacgcgagat
     1921 ccagggccag atcgcaatat cggcgatcga atagtcgccg gcgacatatt ccacttcgcc
     1981 aaggcgccgg tcgagcaccc cccacagccg cttcgcctct ttggcatagc gctcctcggc
     2041 atagctgctc ttgcccgggt tgaacttcac gaaatgatgg acctgcccga gaaagggccc
     2101 cggtcccccc atttgccaca tcagccactc gaccacgcgc cacttcgccg gaaaatcctg
     2161 cggccacagc ttgccggtct tctccgcgag atagagcagg atcgcgcccg actccatcag
     2221 atggagtccg ttgtcggtgt cgacgatcgc ggggatcttg ttgttgggcg cgatcttgag
     2281 gaacgagggc tggaactgat cgcccttggt gatgtcgatg gcatgaacgc ggtatgggag
     2341 cccgacctcc tccagcatga tcgacgcctt ccaaccgttc ggtgtcgtcc aggtgtaaag
     2401 ctcgatcatg gtccctctct tgtgcgaagc gagaacgact cttgtgcgaa gcgagaatga
     2461 ctcttgtgcg aagcgagaat gacgggatgc ccgcgaaaag cgctcctgtc aaaggggcgc
     2521 gggccgattg cttcgcaggt gcgagatcag atgcgcgcaa tttgattcat cggcgcttca
     2581 ttctgcggcc tccctatcgc ccagaacctg tcgcgcagcc gccacacgac ggtgctacct
     2641 cctctggcaa taagcaggcg ctgacaccaa ccgtgcgcgc cgcccaaggg gaggaatgga
     2701 tgcccaattc ggctcaccgc gatcgtcaaa gtccgcacgc cgcgtcatcc cagacgagct
     2761 tcgcgcgcta cgcgattctc tttacgccgc ggcccggcac ggcactcgcg gccttcgggc
     2821 gctcgtggtt cggccgcgcc aatgacggcg tcaccttgca ggccttttcc gatgctggtc
     2881 tcagtgctgc gagcttcgcg cgactcgctc ccatgccggg tcgctacacc ggcctgcatg
     2941 ccctgatcaa agcgccgttt gccccgcgcg ccggaatcgg actcgatgcg atcaagacgc
     3001 ggctcatcaa cttcgcaagg cggcggaacc tattggcgac cggccccttg acgctgtcgc
     3061 gcgacggccg ctttctcgtg ctgcgtccgg tcgaggcgac ccccgggctc gactggcttg
     3121 ccgcccaatg cgtggcgtcg ttggaggatt tcgaatcatc cccggccgaa gccgaaagcc
     3181 aatatcgctt ttcgatcact ctgaccggtc cgctcgagaa agctcatctc gaacgcgtga
     3241 cgcaggcgct ttggcccgtg ctcgaggaga tttgcgcttc cggcgtcacc gtc
//
LOCUS       DASUQP010000075        15689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_851464,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000075 DASUQP010000000
VERSION     DASUQP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15689
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851464"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFY74_03355"
     CDS             complement(<1..390)
                     /locus_tag="VFY74_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX5930778.1"
                     /translation="MRTSHGGKGEGESSPAVARARALRSRMSDAERKLWRMLRDRQFV
                     GAKFRRQVPLGRYVADFLSFDRKVVIEVNGGQHSGKTSDVARDNWFGGQGFTVVRFWN
                     NDVLTNLDGVAVRLLELIDKNTPHPASQ"
     gene            complement(631..2046)
                     /gene="cysS"
                     /locus_tag="VFY74_03360"
     CDS             complement(631..2046)
                     /gene="cysS"
                     /locus_tag="VFY74_03360"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5930779.1"
                     /translation="MSVVLTLYNTLSRKKEAFRPLDAGNVRIYVCGPTVYDFAHIGNA
                     RPVIVFDVLYRLLRHLYGPEHVTYVRNITDVDDKINARAAQDYPDLPLNEAIRRVTAT
                     TERQFHEDMKALGALDPTVEPRATEHIAEMKAMIERLIANGCAYVAEDHVLFHVAAMP
                     DYGKLSGRSLDEMIAGARVEVAPFKKDPMDFVLWKPSKPGEPSWPSPCGIAVPGRPGW
                     HIECSAMAAAHLGKTFDIHGGGIDLVFPHHENEIAQSRCAHATPIMAQLWMHNGFLQV
                     EGEKMAKSLGNFVTVHELLTGWQGYAWPGEALRFNMLRTHYRQPLDWTLVGLDEAHKT
                     LWQWYGDLEGKVTSGGVPGPVLDALLDDLNTPKAMTEMHKLHSAGHWGALRAALGFLG
                     FSCDRAAIVRTASARVEAQLRVSAEAVGERIEARNAARKAKDFKEADRIRDELAAMGV
                     TLKDAKDPKTGELVTTWEVAR"
     gene            2308..3597
                     /locus_tag="VFY74_03365"
     CDS             2308..3597
                     /locus_tag="VFY74_03365"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEX5930780.1"
                     /translation="MSLRRDLWRAVASFVRPAGPTRFGVLALACAAFVAGAEPSLSQG
                     TVSEQQVHCMQLQQELAAAQGGGGANRADLGRIEQQIAQADRVFQGTQAAMEDAGCFD
                     SFFIFGRGLVRSPKCLGMNARVEDARRQLTQLQAQRQVLMGGGGNRRRQAELRDALAR
                     SGCGGQVRPARRGFFDFFGGRDEEEEELPRTPIYRSIDPNGRYRSVCVRLCDGFFYPI
                     HYSVYGSMVGPDAEACQASCAAPAELYVYRNPGQEIEQAVSLNGSAYMDLPVALRYRK
                     EYVKGCSCKHAEYNPTEIMAANKQAGIDTGPMPPAAAPQAPAAAAGAQPPSSLEITGT
                     NPPADPAAPPPAEAAVPPEQQTPAPPAAAAPPPPPASAAVEQAPRPAKPRPRPPAPPA
                     AAPPPPASDSEAGVPPEPAPKPRTAPAAPKASPFAQY"
     gene            complement(3600..4937)
                     /gene="gltX"
                     /locus_tag="VFY74_03370"
     CDS             complement(3600..4937)
                     /gene="gltX"
                     /locus_tag="VFY74_03370"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5930781.1"
                     /translation="MSVTVRFAPSPTGRLHAGNIRTALFNVLFAQARGGRFILRLDDT
                     DRERSTEEFASGIREDLAWLGLDWAVEVRQSDRFALYAKAVEHLKRKGRLYPAYETPE
                     ELELKRKRQLARGKPPVYDRAALKLTAEDRAKLEAGGRKPHWRFLLEQRDVVWDDLVR
                     GRQHVDAASLSDPVLIREDGTYLYTLPSVADDIDLGITHVIRGEDHVANTAPQIQLFE
                     ALDATPPAFGHHSLLVGADGQALSKRDRSLAIEGLRADGIEALAVASYVATLGTSDAV
                     APHASLEALASGFDLAKLSRAPARFDPAELRLLNARLLHALPYSAVTERLRAMGVGGG
                     VEFWEAVRGNLAVLADAAFWWQVVHGPLEPVIADPALCRQAAELLPPEPWDGNVWTVW
                     SGAVKEATGAKGRALFAPLRLALTGREDGPELKQLLPLIGRARASARLEGRTA"
     gene            complement(4941..6614)
                     /locus_tag="VFY74_03375"
     CDS             complement(4941..6614)
                     /locus_tag="VFY74_03375"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5930782.1"
                     /translation="MTKAITDTFKLALAQLNPVLGDIEGNLNKARAVRARAAEEGAGL
                     VAFTELFLTGYPPEDLVLKPAFQKAAREACEALARETNDDGPAVLMGLPWGEGPSVFN
                     AVTLLDGGRIEAVRYKYNLPNYGVFDEKRVFAQGPLPMPIPFRAVNLGVPICEDIWSG
                     DACASLAREGAELLIVPNGSPYWIGKQEFRYDVARLRVAETSLPLAYVNQVGGQDELV
                     FDGASFALNGDGTVAVQMTAWEEALGITEWRREGGQWRCIPGEIAEIERDEAANYLAC
                     VTGLRDYVDKNGFPGVVLGLSGGVDSALCAAMAVDALGAERVHGVMLPYKHTSNESLN
                     DAAATAEALGIQYGIMPIHATVEGLSKDLAKAFAGTTPDATEENLQSRARGTLLMAIS
                     NKFGPMVLTTGNKSEVSVGYATLYGDMNGGFNPIKDLYKGQVYALARYRNRARPKGCL
                     GPEGVVIPENVLTKAPTAELRPGQRDQDTLPPYEMLDDILYCLVEGEMPIRDIVARGH
                     APEIVKKVERMLYLAEYKRRQAAPGVKITARNFGRDRRYPITNRFRERL"
     gene            complement(6771..8162)
                     /locus_tag="VFY74_03380"
     CDS             complement(6771..8162)
                     /locus_tag="VFY74_03380"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX5930783.1"
                     /translation="MAPRWSPDSWRSKPIEQVPVYPDRGVLDQVEEQLAIFPPLVFAG
                     EARELTAKLARVAAGEGFLLQGGDCAESFAEHRADTIRDFFRVFLQMAVVLTYAGGSP
                     VVKVGRIAGQFAKPRSSPAETLDGKELPSYRGDIINGIEFTEAAREADPQRMLMAYRQ
                     SAATLNLLRAFAQGGYANLEHVHRWNLGFVKDSPAGHRYEELAQHIAGTLRFMRACGL
                     NADNTPQLRTTSFYTSHEALLLGYEQALTRVDSTSGEWYATSGHMLWLGDRTRQLDHA
                     HVEYLRGIKNPIGAKIGPGLAPEGLLTLIDVLNPNNEPGRLTLICRFGADKIDKHLPS
                     LIRAVEREGKRVVWACDPMHGNTIKAGSGYKTRPFDLILGEVESFFDIHRSEGTHAGG
                     IHVEMTGQNVTECVGGARAVTETDLSDRYHTHCDPRLNADQSLELAFIMAERLKRERE
                     GRPEPEISIAAGE"
     gene            8289..9029
                     /locus_tag="VFY74_03385"
     CDS             8289..9029
                     /locus_tag="VFY74_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX5930784.1"
                     /translation="MDPRHLEHGHHPRDIAKRLLQGPRVSYLRDWVYGGIDGTVTTFA
                     VVAGVVGAELSTKALLILGAANLFADGFSMAAGNYSGTKAEIEEYEQVRRMEERHVEF
                     APEGEREEIRQIFKAKGFSGDALDSAVDVITATRERWVDTMMTEEHGLPPVSRSPVRA
                     ALTTFLAFLLCGAIPLLPFALGAPGSIWLSTLMTGVTFFAIGSLRSRWSPSPWWRTGL
                     ETFLIGIIAAVVAYIVGVIVAGLVGQIP"
     gene            9071..10237
                     /locus_tag="VFY74_03390"
     CDS             9071..10237
                     /locus_tag="VFY74_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX5930785.1"
                     /translation="MAIKEPSFTIGIEEEYLLVDRNTRDLAQEPPAALLAKCEEALRG
                     QVSPEFLRSQIEVGTRVCTSVQEARDQLVHLRSTVGQIAAEFGLAPIASSTHPFAQWQ
                     SQHHTDKERYNVLAEDLQHVARRLVISGMHVHVGIEDEELRIDLLGQAAYFLPHLLAL
                     STSSPFWQGAQTGLKSYRLSVFDELPRTGIPHQFTSWSEYARTVELLIHAGLIQDATK
                     LWWDLRPSARFPTLEMRVTDICPLVDDSIAIAALYLCILRMLYRLRRQNQRWRHYPPL
                     LVRENRWRAQRYGIEQGMVDFGKGEVVPFAELLEELFELVAEDADHFGCAAEMAHART
                     IVARGTSADRQLARYEAVKALGGSEQAALMAVVDEIVEETMMPPTVRAPKHPSP"
     gene            complement(10553..10768)
                     /locus_tag="VFY74_03395"
     CDS             complement(10553..10768)
                     /locus_tag="VFY74_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930786.1"
                     /translation="MYAKVMRDQFHVQDESIVHAPTGAEFTPVAEESMVIWTGDIGRA
                     LPDGAVYCYAEVLAMMQRVWREMALAS"
     gene            10902..11297
                     /locus_tag="VFY74_03400"
     CDS             10902..11297
                     /locus_tag="VFY74_03400"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5930787.1"
                     /translation="MNTLSNPNGVHKPAGPYHHTVLVSDDARWLVISGQVGMDREGNI
                     ASGARAQSEQAFRNIAACLEANGMRKEDLVKFTVYLTDARFLADYRAARSAVIGDAIQ
                     PASTLLIVAGLASPDFLVEIEAWAAMPQR"
     gene            complement(11541..12296)
                     /locus_tag="VFY74_03405"
     CDS             complement(11541..12296)
                     /locus_tag="VFY74_03405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5930788.1"
                     /translation="MRRLEDRIALVTGAGAGIGRAIAETFAREGAVVIVADYDAEAAA
                     EVAGAIVKWNGTATAHTVDVTDTPQVKALMGEIGKAHGRLDVLVNNAGVGERSDFRHL
                     SDEAWDRVWKTNLDGTVRCAREAFDLLKASGKGAIINLSSIMAAKHTRQMAVYSATKG
                     AVSSLSRSLAVEYAPYGIRVNTLLPGYVETALIGRYISNPMIAKALLTQTPLRRFGTP
                     QDIANAALFLASDEAAYITGAALNVDGGMATTL"
     gene            complement(12283..13677)
                     /gene="gor"
                     /locus_tag="VFY74_03410"
     CDS             complement(12283..13677)
                     /gene="gor"
                     /locus_tag="VFY74_03410"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX5930789.1"
                     /translation="MARYDYDLFVIGAGSGGVRAARLAAELGARVAIAEEYRIGGTCV
                     IRGCVPKKLLVYGSRYGSELAVARGFGWTCEGSRFDWPTLIHNVRREVDRLNGVYTRT
                     LDQTGVDRFLTRASLKSPHEIALADRDGVLSAETILVATGSHPVIPDVPGKEHLISSN
                     ECFELEQLPASIVIIGAGYIGIEFASIFNGLGVAVTVLHRGDQILRDFDLDLRDGLAD
                     AMRNRGIDLRMGTEAASIETEGHGYRVHLARGGSIAADLVMAATGRAPNTSGFGLQHV
                     GVALGWNGHVVVDEFSKSSLDNIYAVGDVTDRKNLTPVAIHEATAFAETLFNAKPTPV
                     DHTSIPTAVFSQPELGTVGLAEDRARELYPKIDVYKQRFRPLKSTVSGRDENMLMKLV
                     IDADSDKILGCHVLGADAAEIVQMAAVALKLGVTKAQFDATMPLHPSAAEELVTMRHR
                     WEPPAPVAGSDAAA"
     gene            complement(13684..14388)
                     /gene="rpiA"
                     /locus_tag="VFY74_03415"
     CDS             complement(13684..14388)
                     /gene="rpiA"
                     /locus_tag="VFY74_03415"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5930790.1"
                     /translation="MVKTDELKEAAARAALDLVTEGMRLGLGTGSTASRFVAALGERV
                     AAGLDVLCVPTSEATREQAVRLGIPLTTLDETPRLDLTIDGADEIDDQLRMIKGGGGA
                     LLREKIVATASDRMVVVADDSKVVSVLGRFPLPVEVVRFGLMSTIGLIEAIAAETGCH
                     GAITLRPGKGEAPFVTDQGNLILDCAFGSIPEPEVLAFSLKRVPGVIEHGLFLGLADL
                     AIVAGKGGVRALRVGA"
     gene            14477..15199
                     /locus_tag="VFY74_03420"
     CDS             14477..15199
                     /locus_tag="VFY74_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX5930791.1"
                     /translation="MFADATIVFDPDGTLVDTAPDLTNALNDVLTRRGLEPVAIEAIR
                     GAVSHGARVMIEEALASAGARGDVDRMLAEFLVHYEANIARESRPYPGALAALDRLAA
                     HGATLAICTNKRERLSRLLLQELGIEHYFSALAGRDTFPVAKPDPGHLTGVIALARGD
                     PSRSVMIGDSDVDLRAAHAASIPAILVSFGYGACPADGVAPGAVIDHFDELEGRLAAL
                     LNGGRGPALTRLKRLNPAADRA"
     gene            15292..15576
                     /locus_tag="VFY74_03425"
     CDS             15292..15576
                     /locus_tag="VFY74_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930792.1"
                     /translation="MLRRVVTGVAIACLMGGVAYAQEQEPVPDSECAQQLTTAQEAVQ
                     DKVDANALSEADQEKIYALLDEADALCTEGKSDEASATLGTVNQMVGKAQ"
BASE COUNT         2658 a         5308 c         5048 g         2675 t
ORIGIN      
        1 ctgcgaagcc gggtgagggg tatttttgtc aatgagctcc agaagccgca cggcgacgcc
       61 atcgagattc gtcagaacgt cattgttcca gaagcgcaca acggtgaaac cctgaccccc
      121 gaaccagttg tcacgcgcga catccgacgt ttttcctgaa tgctgaccgc cattgacttc
      181 gatcaccact tttcgatcga aggagagaaa gtccgctaca tagcggccga gagggacctg
      241 ccgtcggaac ttggcaccaa cgaattgccg gtcgcgcagc atgcgccaaa gcttgcgttc
      301 ggcgtcggac atacggctgc gcagcgctct cgctcttgct accgctggcg aagactcccc
      361 ctcacccttt ccgccatgcg acgtgcgcag agcttcgctc gcgcaagatc gcggcgggaa
      421 gccctctccc acaacctgcc tgcgcgaggg gagagggttc tttgtggagt cgcggccgtt
      481 catcgcgcta cctttttttc ccctctcccc cctgccttcg ccgaagcgaa gctccggctt
      541 cgcgcaggca ggttgtggga gagggtggct tcgcagccgc acgcggctgc gaagccgggt
      601 gagggggtat ttttgtcggg tcgcagcggg tcatcgtgcg acctcccagg tggtcacgag
      661 ctcgcccgtc ttcgggtcct tggcgtcctt cagcgtgacg cccatcgcgg caagctcgtc
      721 gcggatgcgg tccgcctcct tgaagtcctt cgccttgcgc gccgcgttgc gcgcctcgat
      781 ccgttcgccg accgcctcgg cgcttacccg aagctgcgcc tcgaccctcg ccgacgcggt
      841 tcgcacgatc gcggcacggt cgcatgagaa gccgagaaag ccgagcgcgg cccgcaaggc
      901 cccccaatgt ccggcgctat gcagcttgtg catctcggtc atggccttgg gcgtgttgag
      961 atcgtcaagg agcgcatcga gaacgggtcc gggaacgccg cctgaagtca ccttgccttc
     1021 gagatcgcca taccactgcc agagcgtctt atgcgcctcg tcgaggccca ccagggtcca
     1081 gtcgagaggc tggcggtaat gcgtcctcag catgttgaag cggagcgcct cgcccggcca
     1141 ggcgtaaccc tgccagccag tcagcagctc gtgcacggtg acgaagttgc ccaacgactt
     1201 cgccatcttc tccccctcga cctggaggaa gccgttgtgc atccagagct gcgccattat
     1261 cggtgtcgca tgggcgcagc gcgattgcgc gatctcgttc tcgtggtgcg ggaagacgag
     1321 gtcgatgccg ccgccgtgaa tgtcgaaggt cttaccgaga tgggccgcgg ccatggccga
     1381 gcactcgata tgccagccgg gccggcccgg caccgcgatc ccgcacggcg agggccatga
     1441 cggctcgccc ggcttcgacg gcttccacag cacgaagtcc atcgggtcct tcttgaacgg
     1501 ggcgacctcg acccgcgcgc ccgcgatcat ctcgtcgagc gagcggcccg agagcttgcc
     1561 gtagtccggc atcgcggcga cgtggaacag gacatggtcc tcggccacgt aggcgcaccc
     1621 gttcgcgatc aggcgctcga tcatcgcttt catctcggcg atatgctcgg tcgcccgcgg
     1681 ctcgacagtg ggatcgagcg cgccgagtgc cttcatgtcc tcgtggaact ggcgctccgt
     1741 cgttgcggtc acgcgccgga tcgcctcgtt cagcggcaga tcgggatagt cctgcgccgc
     1801 ccgcgcattg atcttgtcgt cgacgtcggt gatgttgcgg acataggtga cgtgctcggg
     1861 gccatagagg tggcgcagca gacggtagag cacgtcgaac acgatcacgg gccgcgcgtt
     1921 gccgatatgg gcgaagtcgt aaaccgtggg cccgcacaca tagatgcgga cgttcccggc
     1981 atcgagcggc cggaaagcct ctttcttgcg gctcagcgtg ttgtaaagcg tcagcacgac
     2041 cgacaagccc cttcgcctcg aggctcgccc ggcggttcgc gtcactctga atgatggtaa
     2101 agggagaacg gcccaggcga gttgatttgc tagcccagaa tgatgccgcg ggaaatgata
     2161 aggatgggtt gcctttgcat catcttggga cgcttatggc cccaatcggc gaaagcgtca
     2221 agcaacccat cttattgatg ccgcattgac cgccgagtct ggccgggcat aatgcgtcgg
     2281 gttgccccct cggggatcgg gaacatcgtg agcctccgtc gtgatctgtg gcgcgctgtc
     2341 gcgtcatttg tgcggccggc cggcccgacg cgcttcggcg tgctggcgct cgcttgcgcc
     2401 gcctttgtcg ccggcgcgga acccagcctc tcgcaaggta cggtcagcga gcagcaagtc
     2461 cattgcatgc agctccagca ggagcttgcc gccgcccaag gcggtggcgg cgcgaaccgc
     2521 gcggacctcg gccgcatcga acagcagatc gctcaggccg atcgtgtgtt ccagggaacg
     2581 caagccgcca tggaggacgc cggctgcttc gacagcttct tcattttcgg ccgcgggctg
     2641 gtgcggagcc cgaaatgcct cggcatgaat gcccgggtcg aggacgcacg ccgccagctc
     2701 acccaacttc aggctcagcg ccaggtgctc atgggtggcg gcggcaaccg gcggcggcag
     2761 gccgagcttc gcgacgctct cgcccgcagc ggctgcggcg gtcaggttcg cccggcgcgc
     2821 cgcggtttct tcgacttttt cggcggccgg gatgaggaag aggaagagct gccgcggacg
     2881 ccgatctatc gcagcatcga cccgaatggc cgctaccggt ccgtttgcgt caggctgtgc
     2941 gacggcttct tctacccgat ccactactcg gtttacggca gcatggtcgg gcctgacgcg
     3001 gaagcgtgcc aggcgagctg cgcggcacct gccgaacttt acgtctatcg caatccgggt
     3061 caggagatcg agcaggcggt gtcgctgaac ggctccgcct acatggatct gccggtcgcg
     3121 ctgcgctacc gcaaggaata cgtgaaaggc tgctcctgca agcacgcgga gtacaatccg
     3181 accgagatca tggcggcgaa caagcaggcg ggaatcgata cgggcccgat gccgccggcg
     3241 gccgcgccgc aggcccctgc cgcagccgcg ggcgcgcagc cgccgtcgag cctcgaaatc
     3301 accggtacaa acccgccggc cgatccggcc gcgcctcctc cggcagaagc cgccgtgccg
     3361 cccgagcagc agacccctgc gcctcccgcc gccgctgctc ccccgccgcc tcccgcctcg
     3421 gcagccgtcg aacaggcgcc gcgccccgca aaaccgcggc cgaggccgcc ggctcctccg
     3481 gccgccgcgc ccccgccgcc ggcttccgat tcagaggcgg gcgtgccgcc cgagcccgcc
     3541 ccgaagcccc ggacagcccc tgccgcgcct aaagcaagcc ccttcgcgca atactgaact
     3601 caagccgttc tgccttcgag ccgcgccgat gcgcgcgcgc gcccgatcag cggcaggagc
     3661 tgcttgagct cgggcccatc ctcccggccg gtgagcgcaa gacggagcgg cgcgaacagg
     3721 gccctgccct ttgcgcctgt tgcctccttc accgcccccg accagacggt ccagacattg
     3781 ccgtcccacg gctcgggcgg gagcaattct gccgcttggc ggcatagcgc agggtcggcg
     3841 atgaccggct cgagcggccc gtgcaccacc tgccaccaga acgcggcatc ggcgaggaca
     3901 gcgaggtttc cgcgcacggc ttcccagaat tccactccgc ctccgacgcc catggcgcgg
     3961 aggcgttcgg tgacggcgct gtagggaagc gcgtgcagga gcctcgcgtt gagaaggcga
     4021 agctcggcgg gatcgaagcg cgccggtgcc cgcgacaact tggcgagatc gaaaccggac
     4081 gcgagcgcct cgaggctcgc atgcggcgcg accgcgtcgg aggttccaag cgttgcgaca
     4141 tagcttgcca cggcaagcgc ctcgatgccg tcggcgcgca agccttcgat ggcgagcgac
     4201 cggtcgcgct ttgagagcgc ctgcccgtcg gcgccgacga gaaggctgtg atgcccgaaa
     4261 gcggggggcg tcgcgtcgag cgcctcgaac aactggatct gcggggcggt attcgccacg
     4321 tggtcctcgc ccctgatgac atgggtgatg ccgagatcga tgtcgtcggc gacgctcggc
     4381 agcgtataga gataggtgcc gtcttccctg atcagcaccg ggtccgacag ggaggcggca
     4441 tcgacatgct gccgtccgcg cacgaggtcg tcccagacga cgtcgcgctg ctccaagaga
     4501 aagcgccaat ggggtttgcg ccccccggcc tccaactttg cccggtcttc cgccgtaagc
     4561 ttcaacgcgg cgcggtcata gacgggcggc ttcccgcgcg cgagctggcg cttgcgcttg
     4621 agctcaagct cctcgggggt ttcgtaggcc gggtagaggc ggcccttccg cttcagatgc
     4681 tcgaccgcct tcgcataaag agcgaagcga tcggactgac ggacctcaac tgcccagtcg
     4741 agcccgagcc acgcgaggtc ctcacggatc ccgctcgcga attcctccgt ggaccgctcc
     4801 cggtcggtgt cgtcgagacg gaggatgaag cggccgcccc gcgcctgcgc gaacagaacg
     4861 ttgaacagcg ccgtgcggat attgccggca tgaagccgcc ccgtcgggct cggcgcgaaa
     4921 cggaccgtga cgctcatcgt ctacagtctc tcgcggaatc tgttggtgat gggatagcgc
     4981 cggtcgcgcc cgaaattcct ggcggtgatc ttcacgcccg gcgccgcctg gcgccgcttg
     5041 tactcggcga gatagagcat gcgctcgact ttcttcacga tctccggggc gtgtccccgc
     5101 gccacgatat cgcggatcgg catctcgcct tcgaccaggc aatagagaat gtcgtcgagc
     5161 atctcgtagg gcggcagcgt gtcctgatcg cgctgtccgg gcctgagctc ggcggtcggc
     5221 gccttggtca gcacgttctc cgggatgacg acgccttccg gtccgaggca gccctttggc
     5281 cgcgcgcgat tgcgatagcg cgcgagcgca tagacctggc ccttgtagag gtccttgatc
     5341 ggattgaagc cgccgttcat gtcgccatag agcgtggcgt agccgaccga gacctcggac
     5401 ttgttgccgg tggtcagcac catcggcccg aacttgttgg agatggccat gagcagggtg
     5461 ccgcgcgcgc gcgactgcaa attctcctcg gtcgcgtcgg gagtggtgcc tgcgaacgcc
     5521 tttgccaggt ccttgctcaa cccctccacg gtcgcatgga tcggcatgat gccgtactgg
     5581 atgccgagcg cctcggccgt cgcggccgca tcgttcaagc tttcgttgct ggtatgcttg
     5641 taggggagca tgacgccatg gacgcgctcc gcgcccagcg catcgaccgc catcgccgcg
     5701 cagagcgccg agtcgacacc ccctgacagg ccgagcacga cgccgggaaa gccgttctta
     5761 tccacgtagt cgcgaagtcc cgtgacgcag gcgaggtagt tcgcggcctc gtcccgctcg
     5821 atctcggcga tctcgcccgg aatgcagcgc cactgtccgc cttcgcgccg ccattcggtg
     5881 atgccgagcg cctcctccca ggccgtcatt tgcacggcga ccgtcccatc gccgttcagc
     5941 gcgaacgagg cgccatcgaa gacgagctcg tcctggcccc cgacttggtt gacataagcg
     6001 agcggcaaac ttgtctcggc gacccgcaac cgcgcgacgt cgtagcgaaa ctcctgcttg
     6061 ccgatccaat agggggagcc gttgggcacg atcaggagct cggcgccttc gcgcgcaagc
     6121 gacgcgcatg catccccgct ccaaatatcc tcgcagatgg gcacgccgag gttcacggcc
     6181 ctgaacggga tcggcatcgg cagcggaccc tgcgcgaaga cccgcttctc gtcgaacaca
     6241 ccgtaattcg gaagattgta cttgtagcgc accgcctcga tgcggccccc gtcgagcagc
     6301 gtcacggcat taaagaccga tggcccctcg ccccacggca gccccatgag cactgccggg
     6361 ccatcgtcat tggtttcgcg cgcgagcgcc tcgcacgcct cccgtgccgc cttctgaaac
     6421 gcgggcttta gcacgagatc ctcgggcgga tagccggtga ggaagagctc ggtgaaggca
     6481 acgaggccgg cgccctcctc cgccgcccgc gcgcggaccg cgcgggcctt attcagattg
     6541 ccctcgatat cgcccagaac cgggttgagc tgcgcgagcg caagcttgaa cgtgtccgtg
     6601 atcgccttgg tcatgaccgg aacttagaac gaaaacgtcc caattgactc gtcttgcccg
     6661 ggcgaagtgc cgggcatcca cgaattttcg tctcaagatc aagacatgga tggccgggac
     6721 aagcccggcc atgacgaagt tgtgactggc gaaccgacca cccgcaaccg ctattcgcct
     6781 gcggcaatcg agatttcggg ctcgggccgc ccctcccgct cgcgcttcag cctctcggcc
     6841 atgatgaagg cgagttcgag cgactggtcg gcgttgagcc tcggatcgca gtgggtatgg
     6901 tagcggtccg atagatcggt ctcggtgacg gctctcgcgc cgcccacgca ttcggtgacg
     6961 ttctggccgg tcatctcgac atggatgccg cccgcgtgtg ttccttcgct gcgatggatg
     7021 tcgaagaagg attcgacttc ccccaggatg agatcgaagg gccgcgtctt gtagccgctt
     7081 cccgccttga tggtgttgcc gtgcatcgga tcgcacgccc acaccacgcg cttgccctcc
     7141 cgctcgaccg cgcggatgag actcggcaga tgcttgtcga tcttgtcggc accgaaacgg
     7201 cagatcagcg tgagccgtcc cggctcgttg ttcgggttga gcacgtcgat gagcgtgagc
     7261 agtccctccg gcgcgagacc ggggccgatc ttggcgccga tcggattctt gatgccgcgc
     7321 agatattcga catgggcatg gtcgagctgg cgggtgcgat cgccgagcca gagcatatgc
     7381 cccgaggtcg cgtaccattc tccggacgtc gaatcgaccc gcgtgagagc ctgctcatag
     7441 ccgagcagaa gcgcttcgtg gctggtatag aagctcgtgg tgcgaagctg cggcgtgttg
     7501 tcggcgttga gcccgcaagc gcgcatgaag cgcaaggtgc cggcgatatg ctgggccagc
     7561 tcctcgtaac ggtggcccgc cgggctgtcc ttgacgaagc cgagattcca ccgatgcaca
     7621 tgctcgagat tggcgtagcc tccttgcgcg aaggcccgca agagattgag cgtggcggcc
     7681 gactgccggt aggccatcag catgcgctgc gggtcggcct cgcgcgccgc ctcggtgaac
     7741 tcgatgccat tgatgatatc gccgcgatag ctcggcagct ccttgccgtc gagcgtttcg
     7801 gccggcgagg agcgcggctt tgcgaactgg ccggcgatgc ggccgacctt caccaccggc
     7861 gaaccgccgg cataggtcag caccaccgcc atctgcaaga acacccggaa gaaatcgcgg
     7921 atggtgtcgg cgcgatgctc ggcgaaactc tcggcgcaat cgcccccctg gaggagaaac
     7981 ccctcgcccg cggccacgcg cgcgagtttc gccgtgagtt cgcgcgcttc gcctgcgaaa
     8041 acgagcggcg ggaagatggc gagctgctcc tcgacctgat cgagcacccc ccggtcggga
     8101 tagaccggga cctgctcgat cggcttcgag cgccaactgt cgggactcca tcgaggcgcc
     8161 atcacggctc tcccttcaaa gtgatcgcta tataggcgat cggcggaacc ctggctagcc
     8221 gcggggcttg attgcctgcc tctttcgagc gtgccacata tcccttaacg cacggcaagg
     8281 gagctagcat ggacccacgg cacctcgaac acggacatca cccgcgcgat atcgccaaac
     8341 gcctcctgca aggcccgcgc gtcagttatc tgcgggattg ggtctatggc ggcatcgacg
     8401 gcacggtgac cactttcgcc gtggtggcag gggtggtcgg cgcggagctg tcgaccaagg
     8461 ccctgctcat cctcggcgcc gccaatctgt tcgccgacgg cttctcaatg gcggccggaa
     8521 actactccgg aacgaaagcc gagatcgagg aatacgagca ggtacgccgg atggaggagc
     8581 ggcatgtcga gtttgcgccg gaaggcgagc gcgaggagat ccgccagatc ttcaaggcca
     8641 agggtttctc cggcgacgcg ctcgacagcg ccgtggatgt cattaccgcc acgcgcgagc
     8701 gctgggtcga cacgatgatg acggaggagc acgggctgcc gccggtcagc cgttcgccgg
     8761 tgcgggcggc gctgacgacg tttcttgcct ttctcctctg cggcgcgatt ccgttgctgc
     8821 cattcgcgct cggcgcgccc ggttcgattt ggctctcgac tctgatgacc ggcgttacct
     8881 ttttcgcgat cggctcgctc agaagccgct ggtcgccgtc gccgtggtgg cggaccgggt
     8941 tggagacctt cctgatcggg atcatcgccg ccgtcgtcgc ctatatcgtc ggcgtaatcg
     9001 tcgccggtct ggtcggacag ataccgtaag ccctaccgcg ggcgctgttc ttcgtgccac
     9061 catggaagca atggcgatca aggaaccaag tttcaccatt ggcatcgagg aggaatacct
     9121 cctcgtcgat cgcaataccc gcgatctcgc ccaggagccg ccggcggcgc tgctcgccaa
     9181 atgcgaagaa gcgctgcgcg gacaggtcag ccccgagttc ctgcgctcgc agatcgaggt
     9241 cggcacgcgc gtatgcacat ccgtgcagga ggcgcgcgac cagctcgtcc acctgcgcag
     9301 cactgtcggc cagattgccg ccgagttcgg gcttgcgccg atcgcgtcct cgacgcaccc
     9361 cttcgcgcaa tggcagagcc agcatcacac cgacaaggaa cgttacaatg tgctggccga
     9421 ggatctccag catgtggcgc ggcggctcgt gatctccggc atgcacgtgc atgtcggcat
     9481 cgaggacgag gagctcagga tcgatctgct cggccaggcg gcctatttcc tgccgcatct
     9541 ccttgcgctc tcgacctcct cgccgttctg gcaaggcgcg cagaccggac tgaaatccta
     9601 tcgcctgtcg gtcttcgacg agctgccccg caccggcata ccgcatcaat tcacgagctg
     9661 gagcgagtat gcccgcacgg tcgagcttct gatccatgcc ggcttgatcc aggatgcgac
     9721 caagctctgg tgggacttgc ggccctccgc gcgttttccc acgctggaaa tgcgcgtcac
     9781 cgatatctgc ccgcttgtcg acgacagcat tgccatcgcc gcgctctatc tctgcatctt
     9841 gcgcatgctc tatcgcctgc gccggcagaa ccagcgctgg cgccactacc cgccgctgct
     9901 cgtgcgcgag aaccgctggc gcgcgcagcg ctacggcatc gagcagggca tggtcgattt
     9961 cggcaagggc gaagtggttc ccttcgccga gctgctcgaa gagctgttcg agctcgttgc
    10021 cgaggatgcc gaccatttcg gctgcgcggc cgagatggcg catgcccgca ccatcgtggc
    10081 gcgcggcacc agcgccgacc ggcaactcgc ccgttacgag gcggtgaaag cgctcggcgg
    10141 ctccgagcag gcggcgctga tggccgttgt cgacgagatc gtcgaggaga cgatgatgcc
    10201 gccgaccgtc cgagcaccga aacacccctc cccttgaagt gcgacgaact ttccctcccc
    10261 cccttgacgg ggagcacacg cgcccgtggc gcgtggaggg ggtgacgcag aagccattga
    10321 acaatggaga ggagtctttg cgttcgaccc ccctccctga ccctccccct caagggggag
    10381 ggaatgagtt caacggagag gtgacgggtt cgtgatttgt cataccggcg aaagccggta
    10441 tccagagcca cgaacgcggt gcatgccgcc tctggattcc ggcttgcgcc ggaatgacgg
    10501 cttaccctgc cacgcggagc tcatccctgg ccctgattgc agggcggcga gactaggagg
    10561 ccaaggccat ctcccgccac accctttgca tcatcgccag cacttcggcg tagcaataga
    10621 ccgcgccatc cggcagggcc ctgccgatgt ccccggtcca aatcaccatc gactcttcgg
    10681 cgaccggcgt aaactccgca ccggtcggcg cgtggacgat cgactcgtct tgcacgtgaa
    10741 attggtcccg catcaccttt gcgtacatca gattccctct cccctaagcg cgtgtgattc
    10801 gcacgagccc atcattgcag catgttagga ttaaggagcg attgcggcgg gaaatcgcaa
    10861 ccgaaaaatg cgtaaattca acaaggaacc aagagcaccc tatgaacacc ctctcgaacc
    10921 cgaacggcgt ccacaagccg gccggcccct atcatcatac ggtcttggta tccgatgacg
    10981 cccgctggct ggtgatctcc ggacaggtgg gaatggatcg cgagggcaat atcgcgagtg
    11041 gcgcaagagc gcagtccgag caggcgtttc gcaacatcgc cgcctgtctc gaggcgaacg
    11101 gcatgcgcaa ggaggacctc gtcaagttca cggtctatct caccgacgct cgcttcctgg
    11161 ccgactaccg ggcggcgcga tctgcggtca tcggcgacgc cattcaacct gcctcgacgc
    11221 ttctgatcgt cgccggcctt gcgagccccg atttccttgt cgagatcgag gcctgggccg
    11281 ccatgccgca acgctagatc ggcggtaggc cagtcgaagc gaatattcga gaagaacgcg
    11341 tcataccggc gaaagccggt ccgcatgccg cttcaaacac ccgctctccc cttgtgggag
    11401 agcacgcgct cgcgaggcga gcgcggtgag gggtaagcgg cgcagccgcg tcgaaacgaa
    11461 aacccctcac ccgccccggc ttttgccggg gcaccctctc ccacgggggg agagggattg
    11521 gcgttcgcgc gacgggatgg ctagagcgtg gtcgccatgc cgccatcgac attgagggcg
    11581 gcgcccgtga tgtaggccgc ctcgtcggag gcgaggaaca gcgcggcatt ggcgatgtcc
    11641 tgcggggtgc cgaagcgccg gagcggggtt tgcgtgagca gcgccttggc gatcatcggg
    11701 ttggagatat agcgcccgat cagcgccgtc tcgacatagc ccgggagcag cgtgttcacg
    11761 cggatgccgt agggggcgta ttcgaccgcg aggctccgcg acagcgacga caccgcgccc
    11821 ttcgtcgccg aatagaccgc catctggcgc gtatgcttgg ccgccatgat cgaggagaga
    11881 ttgatgatcg cgcccttgcc cgaagccttg agcagatcga acgcctcgcg cgcgcagcgc
    11941 accgtcccgt cgagattggt cttccacacc cggtcccagg cctcgtcgga gagatggcgg
    12001 aagtcggacc gctcgcccac gcccgcgttg ttgacgagca catcgagccg gccatgcgcc
    12061 ttgccgatct cgcccatgag cgccttcact tgcggcgtgt cggtgacatc caccgtgtgc
    12121 gcggttgccg tgccgttcca cttgacgatg gcgccggcga cctcggctgc cgcctccgcg
    12181 tcgtagtctg ccacgataac gacggcaccc tcgcgcgcga acgtctcggc aatggcgcgg
    12241 ccgatccctg cccctgcgcc ggtgacgagc gcgattctgt cctcaagccg ccgcatccga
    12301 ccccgccacc ggcgccggcg gctcccatct gtggcgcatc gtcacgagct cctcggcagc
    12361 gctcggatgg agcggcatgg tggcgtcgaa ctgcgccttg gtgacgccga gcttgagcgc
    12421 caccgcggcc atctgcacga tctccgcggc atccgctccc agcacgtggc agcccaatat
    12481 cttgtcggag tcggcgtcga tgacgagctt catcagcata ttttcgtcgc ggccgctcac
    12541 cgtggatttc agcggccgga accgctgctt gtagacatcg atcttgggat agagctcgcg
    12601 cgccctgtcc tccgcgagcc cgaccgtgcc gagctcaggc tgcgaaaaaa cggcggtggg
    12661 gatggaggta tgatctaccg gggtcggctt cgcgttgaac aaagtctcgg cgaaggcggt
    12721 cgcctcgtgg atggcgaccg gcgtcagatt cttacggtcg gtcacgtcgc cgacggcata
    12781 gatattgtcg agcgaggatt tcgagaactc atccaccacg acatggccgt tccagccgag
    12841 tgccacgcca acgtgctgaa gcccgaaccc tgacgtgttc ggcgcccgtc ccgtcgccgc
    12901 catgacgaga tcggccgcaa tcgagccgcc gcgggcaaga tgcacccgat agccgtgccc
    12961 ctccgtttcg atcgatgccg cttcggtgcc catgcggagg tcgatgccgc gattgcgcat
    13021 cgcatcggca agcccgtcgc gaaggtccag gtcgaagtcg cgcaagatct gatcgccgcg
    13081 gtggagcacg gtcacggcga cgccgagccc gttgaagatc gaggcgaact cgataccgat
    13141 atagccggcc ccgatgatga cgatgctcgc ggggagctgc tcgagctcga aacattcgtt
    13201 cgagctgatc agatgctctt ttcccggcac gtcgggaatg acgggatgac ttccagtggc
    13261 aacgaggatc gtttcggccg agaggacgcc gtcgcgatcg gcgagcgcaa tctcatgcgg
    13321 gcttttgagc gaggcgcggg tgagaaagcg atcgacgccc gtctggtcca acgttcgtgt
    13381 gtagacgccg ttcaggcggt cgacttcgcg ccgcacgttg tggatcaagg tcggccagtc
    13441 gaaacgcgat ccctcgcagg tccacccgaa cccgcgcgca accgcgagct cgctcccgta
    13501 acggctgcca tagacgagga gcttcttcgg cacgcagccg cggatcacgc aggtgccgcc
    13561 gatgcgatat tcctcggcga tggcgacgcg cgcgccgagt tcggcggcaa ggcgcgccgc
    13621 gcgcaccccg ccggagcccg cgccgatgac gaacaaatcg tagtcgtacc gagccacgtg
    13681 gcgtcaggct cccacgcgca gcgctcgaac gccgcctttg ccggccacga tagccaaatc
    13741 ggcgaggccg agaaacaggc catgctcgat cacgcccggc acacgcttca gcgagaaggc
    13801 gagcacctcc ggctccggga tgctgccgaa ggcgcagtcg aggatgaggt tgccctggtc
    13861 ggtgacgaac ggcgcctcgc cctttcccgg cctcagcgtg atcgcgccgt ggcacccggt
    13921 ctcggcggcg atggcctcga tcagcccgat cgtcgacatg agcccgaagc gcacgacctc
    13981 gaccggcaac gggaagcggc cgagcaccga gacgaccttg ctgtcgtcgg ccacgaccac
    14041 catgcgatcc gacgccgtcg ccacgatctt ctcgcggagc agcgcgccgc ccccgccctt
    14101 gatcattctg agctggtcgt cgatctcgtc ggcgccgtca atcgtaagat cgaggcgggg
    14161 cgtctcgtcg agcgtggtga gcgggatgcc gagccgcacc gcctgctcgc gcgtggcctc
    14221 cgaggtcggg acgcagagca catcgaggcc ggctgccacc cgctctccga gagccgcgac
    14281 gaagcgggaa gcggtcgaac cggtgccgag tccaagccgc atgccttcgg taacgagatc
    14341 gagcgcggcg cgggcggcgg cttccttgag ttcgtccgtc ttgaccaacg accctctccg
    14401 ccaacctgcg gcacgaggct caaccctcta cgctgccgct tgccaggccg caaggcgcgt
    14461 gtgacagagt gctgccatgt tcgccgacgc caccatcgtg ttcgatcccg acggcaccct
    14521 ggtcgacacg gcgccggacc tgaccaacgc gctgaacgac gtgctgaccc gccgcggtct
    14581 tgagccggtt gcgatcgagg ccatccgcgg cgccgtgagc cacggcgccc gggtcatgat
    14641 cgaggaagcg cttgcgagcg ccggcgctag aggcgacgtg gaccggatgc ttgccgagtt
    14701 cctagtccat tacgaggcca atatcgcccg cgagagccgc ccctatccgg gcgcgctcgc
    14761 cgcgctggac cgcctcgccg cgcatggggc aacgctcgcg atctgcacca acaagcgcga
    14821 gcgcctaagc cgccttctgc tccaagagct cggaattgaa cattatttca gcgcgctcgc
    14881 cggacgcgac acgtttcccg tggcaaagcc cgacccgggc catctcaccg gcgtcatcgc
    14941 acttgcccgc ggagacccct cacggtccgt catgatcggc gacagcgacg tcgatcttcg
    15001 ggcggcgcac gcggcctcca ttcctgcgat cctcgtcagc ttcggctatg gcgcctgccc
    15061 cgcagacggc gtggcacccg gagccgtcat cgatcatttc gacgagctcg aggggcgcct
    15121 cgcagccctc ctgaacgggg gccgcgggcc cgcgctcaca cggctgaaac gcctcaatcc
    15181 cgccgcagat cgggcctaac tccacccggc cgctctcaat gaagagccaa ggcgaaagag
    15241 ggtttcgcat gcccttccgg ggggacttgt tcagacgcga gggagaaaga gatgctgaga
    15301 cgtgtggtga ccggcgttgc catcgcctgc ctcatgggtg gggtggctta cgcgcaagaa
    15361 caggaacccg tacccgacag cgagtgcgcc cagcagctca ccaccgcgca agaggccgtg
    15421 caggacaagg tcgacgccaa cgccttgagc gaggccgacc aggagaagat ctacgcgctg
    15481 ctcgacgagg ccgacgcgct ttgcaccgag ggcaaatccg acgaagcgag cgcgacgctc
    15541 ggcaccgtca accagatggt cggcaaggcc caatagatgt tcgagcgttc ggcgcgccgg
    15601 cgcgggcggc gcgattcgga gagcatgatt tagcccgatt gaaccacgct cccgaccccc
    15661 accccggccc tccccccttg caggggggg
//
LOCUS       DASUQP010000076         6932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1396480, whole genome shotgun sequence.
ACCESSION   DASUQP010000076 DASUQP010000000
VERSION     DASUQP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396480"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFY74_03430"
     CDS             complement(<1..570)
                     /locus_tag="VFY74_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5930793.1"
                     /translation="MVSTGRPMIGLALGGGAARGWAHIGVLKSLVSAGLYPDIVAGTS
                     MGAVAGACYVTGRLGALEEFACSLTRRRLFGFLDFNFGGSGLISGQRLSARLEHHLRQ
                     FDIERLERKFVAVATELGTGHEVWLNKGSLVNALKASFALPGIFRPVQINGRWLIDGA
                     LVNPIPVSVCRALGARIVIAVNLSSDPFGR"
     gene            complement(686..1138)
                     /gene="hisI"
                     /locus_tag="VFY74_03435"
     CDS             complement(686..1138)
                     /gene="hisI"
                     /locus_tag="VFY74_03435"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX5930794.1"
                     /translation="MSKEAPFPAPDSKTASEEGTTFAPRFDKDGLLPVVTTSAKTGEL
                     LMLAYMNEEALARTIETGEAHYWSRSRGRLWRKGEESGNTQRVVEMRTDCDQDAVWLI
                     VEMGGAEAACHTGRNSCFYRALPVGTAPGGGLSLRFVDSKPRFDPGEG"
     gene            complement(1162..1806)
                     /gene="folE"
                     /locus_tag="VFY74_03440"
     CDS             complement(1162..1806)
                     /gene="folE"
                     /locus_tag="VFY74_03440"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5930795.1"
                     /translation="MDATVKKLRTAPQAQIGRETGIVRPSREEAEEAVRTLIAYAGDD
                     PDREGLRETPRRVTSAYDEFFAGYREEPEEVLSRTFDEVAGYDDIVMLRDIELHSHCE
                     HHMIPFVGKAHVAYFPTDRVVGISKLARVVEVFARRLQTQETMTAQIAETIDKALKPK
                     GVAVMIEAVHQCMSIRGVKKPDVATITTQFTGIFKEDPAQQARFMMLATERGRG"
     gene            2003..2476
                     /locus_tag="VFY74_03445"
     CDS             2003..2476
                     /locus_tag="VFY74_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX5930796.1"
                     /translation="MALPEDIYSQRILELAAALPRTARLAVPEATAEAYSKLCGSRVT
                     VDLTMQGDVVSDYGQSVKACLLGQSAASVMGREIVGSNAEELRGVGAAMRKMLKAGGP
                     PPQGRWADLSVLEPVRDYKARHASTLLVFDAVEEAIAKIEASRRESASRAHAEPA"
     gene            2500..2850
                     /gene="yidD"
                     /locus_tag="VFY74_03450"
     CDS             2500..2850
                     /gene="yidD"
                     /locus_tag="VFY74_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5930797.1"
                     /translation="MSSRDEERFMHKSRLDRVIKAPLLGVLAAYRYTVAPLLGVNCRH
                     LPSCSDYAREAIEKNGAWKGGWLTLARLCRCHPWGSHGFDPVPDLDGERHPFAPWRYG
                     RWRIYARDGTADSR"
     gene            2915..4864
                     /gene="thrS"
                     /locus_tag="VFY74_03455"
     CDS             2915..4864
                     /gene="thrS"
                     /locus_tag="VFY74_03455"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5930798.1"
                     /translation="MSQISLTFPDGSARDFKSGVSGRDVAEQISKSLAKKAVAVQLDG
                     KTRDLAEPIKVNARIRILTRDDAESLELIRHDAAHVMAEAVQALYPGTQVTIGPVIEN
                     GFYYDFARDQPFTPDDLPKIEAKMREIIARDSPFTCEVMDRKAAKKLFRDKGEIFKLE
                     LIDAIPEGEAIKIYSQEKWLDLCRGPHMTSTGKVGKGFKLLKIAGAYWRGDSSKPMLQ
                     RIYGTAWASEEDLKAYLHQLEEAEKRDHRRLGREMDLYHFQEEAPGAVFWHPKGWALF
                     TALIAYMRRRQQAWGYVEVNSPDMMERTLWEQSGHWEKFGDLMYVTETPDERVYCCKP
                     MNCPGHVQIFKHGLKSYRDLPLRIAEFGKVHRYEPSGALHGLLRVRHFTQDDAHIFCT
                     EDQITEECVKLNAQLLAIYRDFGFTDVRIKFADRPDKRVGADAVWDKAEAALKRAVEA
                     AGLTYEFNPGEGAFYGPKLEYALRDTIGRDWQCGTIQVDLNLPARLGAFYIGPDGEKR
                     VPVMIHRAMFGSLERFTGILIEHYAGHLPLWLAPIQAVVATIVSEADPYALEVLSALQ
                     GAGLRAEADLRNEKINYKVREHSLAKVPVLLVAGRREAEERTVSVRRLGSQEQQVMPL
                     DAAIRALSAEAVPPDLRESAESKAA"
     gene            4877..5470
                     /locus_tag="VFY74_03460"
     CDS             4877..5470
                     /locus_tag="VFY74_03460"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX5930799.1"
                     /translation="MMRTRPILTIGHSRHSWERFAALLAGAGIETLADIRSVPRSRFS
                     PHFNKDALTAALAALNVAYVFLGKELGGRPQDPQMYSGGVADYEKMAAAPEFRLGLTR
                     LVEAAAERSLAMMCSEADPLECHRCLLVGRALAEGGSDVRHILASGEIMTHAEAEDRL
                     LALENLIEADLLATSREERLAQAYRSRSRKSAYAQAQ"
     gene            complement(5849..>6932)
                     /locus_tag="VFY74_03465"
     CDS             complement(5849..>6932)
                     /locus_tag="VFY74_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930800.1"
                     /translation="RPPAKMALAPSAPEGGAPSAPVAPKPSTQSMAAQVQLPVPVIFK
                     ALVSEAGPRLQSGLTWRVYESKSLPGGVGYKLVSTHRDPMPTAALLPGEYLVNAAYGL
                     SNLTKKIKVESGRSLEETFVLNTGGLKLAALLPNGEPLPESAARYDILSDEEDQFGNR
                     PMVLRGAKPGIVIRLNAGAYRIECHYGDANATVKADITVEPGKVTEASLKQTGAKTTF
                     KLVQTLGDEALADTKWTILTSAGDVVKENAGALPTHILAPGSYAVVADHAGESFTRKF
                     SIEPGEAKQIEVVVEDGPSSAEEIKALTDPPEPPPAPAGGVIAGDGTTPSPNAGAAFD
                     GFSPNVPADPNAPLINPGILLRGPSR"
BASE COUNT         1202 a         2211 c         2292 g         1227 t
ORIGIN      
        1 tctgccgaag gggtcgctgc tcaagttgac ggcgatgacg atgcgggcgc caagcgcgcg
       61 gcacaccgaa accgggatcg ggttgacgag ggccccgtcg atcagccagc ggccgttgat
      121 ctggaccggg cggaagatgc caggcagcgc gaacgaagcc ttcagcgcat tgaccagact
      181 tcccttgttg agccagacct cgtgtccggt gccgagttcg gtggcgaccg ccacgaattt
      241 ccgctccaga cgttcgatgt cgaattgccg cagatggtgc tcgagccgcg cgctcagacg
      301 ctggccggag atcagccccg acccgccgaa attgaaatcg agaaaaccga acagccggcg
      361 gcgcgtcagg ctgcaggcga actcctcaag ggcgcccagc ctgccggtga cgtaacaggc
      421 gccggccacg gcccccatgg aggtgccggc gacgatgtcg ggataaagcc cggccgagac
      481 caggcttttc agcacgccga tatgggccca gccgcgggcc gccccgccac ccaaggcaag
      541 gccgatcatg ggtcgtcccg tgcttaccat ggctcagaat ccatgccgca tgccctgcgc
      601 tccccgcatg accgcctgcg agccaaaccc gggagaaggg tataccgaac tctttaaggt
      661 tccctttgcg gcgccagcat gacgcctaac cctcacccgg gtcgaatcgc ggcttggagt
      721 cgacgaagcg caaggataga ccgccgcccg gcgcggtccc aacaggcagt gcacggtaga
      781 aacaggagtt ccgccccgta tgacaagcgg cttcggcgcc ccccatctcg acgatgagcc
      841 agacggcgtc ctggtcgcag tcggtgcgca tctcgacgac gcgctgggtg ttgccgcttt
      901 cctcgccttt ccgccatagg cgcccgcgcg agcgcgacca gtaatgcgct tccccggttt
      961 cgatggtccg ggcgagagcc tcttcgttca tgtaggcgag catcagcagc tcgccggtct
     1021 tggcggaggt cgtcaccacg gggagaaggc catccttgtc gaatcgcgga gcgaaggtcg
     1081 tgccttcctc gctcgcggtc ttgctgtcgg gggcggggaa aggggcttcc ttgctcaccg
     1141 cgatatcctt gggcgagcgc gtcagccgcg gccgcgttcg gtcgcgagca tcatgaaacg
     1201 cgcctgctgg gcggggtctt ccttgaagat gccggtgaac tgcgtggtga tggtggcgac
     1261 gtcgggcttc ttcacgccgc ggatcgacat gcattgatgc acggcctcga tcatcaccgc
     1321 cacgcccttc ggcttcagtg ccttgtcgat ggtctcggcg atctgcgcgg tcatggtctc
     1381 ctgcgtctgc aagcggcgcg cgaacacctc gaccacgcgg gcgagcttgg agatccccac
     1441 cacgcggtcg gtcgggaaat aggccacgtg ggccttgccg acgaacggga tcatgtggtg
     1501 ctcgcaatgg gagtgcagct cgatgtcccg gagcatgacg atgtcgtcgt agccggcgac
     1561 ctcgtcgaag gtgcgcgaca gcacctcttc cggctcctcg cgatagccgg caaagaattc
     1621 gtcataggcg gaggtgaccc ggcgcggcgt ttcgcgcaag ccctcgcggt caggatcgtc
     1681 gccggcgtag gcgatcaagg tgcggacggc ctcctcggct tcctcgcgcg acgggcgcac
     1741 aatgccggtc tcgcgcccga tctgggcttg cggcgcggtc cgcagcttct tgacggtagc
     1801 gtccatgact cctcgggact ttccttgcct taccgccttt ttcggctggt tcaaccctat
     1861 tcagttgggg acagtatacc cggctcgtca acctttgatc gcgccgtcgt gccttcggac
     1921 ccctctgatc ctggggccgg gctgcatata taatgcggca gatatgcccg caaggcgagg
     1981 ctcataccga tcgaaggcac gcatggcgct tcccgaagac atctatagtc aacgcatcct
     2041 tgagcttgcg gcggcgctgc cgcggacggc acggctcgcc gtgcccgagg ccacggcgga
     2101 agcctattcc aaactctgcg gcagccgggt aacggtcgat ctgacgatgc aaggcgacgt
     2161 ggtgagcgac tatgggcaga gcgtgaaggc ttgcctcctg ggccagtcgg cggcgtcggt
     2221 catgggacgg gaaatcgtgg gctcgaacgc cgaggaattg cgcggggtcg gcgccgccat
     2281 gcgcaagatg ctgaaggcgg gaggaccgcc gccgcaaggg cgatgggctg atctttctgt
     2341 gcttgagcct gtgcgcgact acaaggcccg ccatgcctcg acccttctcg tgttcgacgc
     2401 ggtcgaagag gccattgcca agatcgaggc gagtcgccgg gagagcgcaa gccgcgctca
     2461 tgccgaaccg gcttgagcgc aaggcgcatc ttccaatcga tgagcagccg cgacgaggag
     2521 cgcttcatgc ataagagccg gctcgaccgc gtcatcaagg ctccgttgct tggcgtgctt
     2581 gccgcctatc gttacaccgt cgcgccgctg ctcggcgtca attgccggca tctcccctcc
     2641 tgctcggact acgcgcgcga ggcgatcgag aagaacggcg catggaaagg cggctggctc
     2701 acgctcgcac gactctgccg ctgccatcct tggggcagcc acggcttcga ccccgtgccc
     2761 gatctcgacg gcgagcgcca tcccttcgcg ccatggcgct atggaaggtg gcggatttac
     2821 gcgcgtgacg ggacagccga ttcccggtaa aagctctgta cctgatcacc agtctcttac
     2881 ctgcaccggt acgcaggagt gagaggagca tcgcatgtcg caaatctccc tgacatttcc
     2941 cgatggctcg gcccgcgatt tcaagtccgg ggtctcgggg cgcgacgttg ccgaacagat
     3001 ctcgaagtcg ctcgccaaga aggcggtcgc ggttcagctc gacggcaaga cgcgcgacct
     3061 cgccgaaccg atcaaggtca atgcgcgcat ccgcatcttg acgcgcgacg acgcggaatc
     3121 gctcgagctc atccgccacg acgcggcgca tgtgatggcg gaggccgtgc aggcgcttta
     3181 ccccggcacg caagtcacca tcggccccgt catcgagaac ggcttctatt acgacttcgc
     3241 gcgcgaccag cccttcacgc ccgacgacct gcccaagatc gaagccaaga tgcgcgagat
     3301 catcgcccgc gactcgccgt tcacctgcga ggtgatggac cgaaaagcgg cgaagaagct
     3361 gtttcgcgac aagggcgaga tcttcaagct cgagctgatc gacgccattc ccgagggcga
     3421 ggcgatcaag atctattcgc aagagaaatg gctcgacctc tgtcgcggac cgcatatgac
     3481 ctcgacgggg aaggtcggga agggcttcaa gctgctcaag atcgcgggcg cctactggcg
     3541 cggcgattcc tccaagccca tgctccagcg catctacggc acggcctggg cgagcgaaga
     3601 ggatctcaag gcctatctgc accagctcga agaggccgag aagcgcgacc atcgccgcct
     3661 gggacgcgag atggacctgt accatttcca ggaagaggcg ccgggcgccg tgttctggca
     3721 tcccaagggc tgggcgctgt tcacggcgct cattgcctat atgcgccgcc gccagcaggc
     3781 gtggggctat gtcgaggtca attcgcccga catgatggag cgcacgctgt gggaacagtc
     3841 gggccattgg gagaagttcg gcgacctcat gtacgtcacc gaaacgccgg acgagcgcgt
     3901 ctattgttgc aagccgatga actgccccgg ccatgtgcag atcttcaagc acggcctcaa
     3961 aagctatcgc gacctgcctt tgcgcattgc cgagttcggc aaggtgcatc gctacgagcc
     4021 gtcaggcgcg ctacacgggc ttctgcgcgt gcgccatttc acgcaagacg acgcgcatat
     4081 cttctgcacc gaggaccaga tcaccgagga atgcgtgaag ctgaacgcgc agctgcttgc
     4141 gatctatcgc gatttcggct tcaccgatgt ccgcatcaag ttcgccgaca ggcccgacaa
     4201 gcgtgtcggc gccgacgccg tatgggacaa ggcggaagcc gcgctgaagc gcgccgtcga
     4261 ggcggcgggg ctgacctacg agttcaatcc gggcgagggg gcgttctacg ggcccaagct
     4321 ggaatatgcg ttgcgcgaca cgatcggccg cgactggcaa tgcggcacga tccaggttga
     4381 cctcaatctg cccgcgcgcc tcggcgcctt ctatatcggg cctgacggag agaagcgggt
     4441 tccggtgatg atccaccgcg ccatgttcgg gtcgctggag cgcttcaccg gcattctcat
     4501 cgagcattac gcgggccatt tgccgctctg gcttgcgccg atccaggccg tggtcgcgac
     4561 catcgtgtcg gaggccgacc cctatgcctt ggaggtgctc agcgcgcttc agggggcagg
     4621 gctccgcgcc gaggccgatc tccgcaacga gaagatcaac tacaaggtcc gcgagcactc
     4681 gctcgccaag gttccggtgc tgctcgtcgc cggaaggcgc gaggcggaag agcgcaccgt
     4741 gtcggtgcga cggctcggca gccaggagca gcaggtgatg ccgctcgatg ccgcaatccg
     4801 cgccctctcg gccgaagcgg tgccgcccga tctgcgggag tcggccgaga gcaaggcggc
     4861 ttagtcctcc gccggcatga tgcggacccg accgatcctc acgattggcc actcgcggca
     4921 ttcctgggaa cggttcgcag cgctccttgc cggggcgggc atcgagacgc tcgccgatat
     4981 ccgttcggtg ccgcgctcgc gcttttcccc gcatttcaac aaggacgctt tgactgccgc
     5041 gctcgcggcg ctaaacgtcg cttacgtatt cctcggcaag gagcttggtg ggcgcccgca
     5101 agatccgcaa atgtattcag gcggcgtggc ggattacgag aagatggccg cggcgccgga
     5161 atttcgcctc gggctgacgc ggctcgttga ggcggcggcg gagcgcagtc ttgccatgat
     5221 gtgctcggag gccgatccgc tcgaatgtca tcgatgcctg cttgtcggtc gcgccttggc
     5281 ggagggcggt tccgatgtca ggcatattct cgcctccggc gaaatcatga cccacgcaga
     5341 ggcggaagac cggctgcttg cgctcgagaa tctgatcgag gcggatttgc tggcgacctc
     5401 gcgcgaagaa cgcctcgcgc aggcctaccg ctcccgctcc cgcaaatccg cctacgcaca
     5461 agcgcaatga ttcatttgct gctctcccct tgtgggagag cacgcgagcg tttttccgcg
     5521 ctcgcgggga ggggtaagcc gcgaagcggc gtcgaaaaga attcacccca cacccttccc
     5581 gccactcgcc gcgcatcgcg cgtcgggcgg gaagccctct ccctcgaggg gagaggggcg
     5641 cgtctctttt tcaaacctcc tccttgtggg gaggtccgac gctgggggtg gggcgtcacg
     5701 ctcaagcggc aaacgtgatc gcgaattgaa acacataccc gactgggcga agcttcagcg
     5761 gagcgaaagc tgggctggtc gtcgccactt gaaggtcagg ccacccggcg cgccaatttc
     5821 cccacgaggg ggaggtttaa aataggtttt agcgcgaggg gccgcggagc aggatgccgg
     5881 ggttgatcag cggcgcattg ggatcggccg ggacgttcgg cgagaagccg tcgaaggccg
     5941 cgcctgcgtt cggcgacggt gtcgtgccat cacctgcaat cacgccgcct gcgggcgccg
     6001 gcggcggttc gggcggatcg gtcagcgcct tgatctcttc cgccgaactc ggcccatcct
     6061 cgaccacgac ctcgatctgc ttggcctcgc ccggttcgat gctgaacttg cgcgtaaagc
     6121 tctcgcccgc atggtcggcg accacggcat agctgccggg ggcgaggata tgggtgggga
     6181 gcgcgccggc attctccttc accacgtcgc cggccgaggt gaggatggtc cacttggtgt
     6241 cggcgagagc ctcgtcgccg agcgtctgca cgagcttgaa ggtggtcttc gcgccggtct
     6301 gctttagcga agcctcggtc accttgccgg gctcgaccgt gatgtcggct ttgacggtgg
     6361 cattggcgtc gccatagtgg cactcgatgc gataggcccc ggcattgagc cggatcacga
     6421 tgccgggttt ggcgccacga agaaccatcg ggcggttgcc gaactggtcc tcttcgtcgg
     6481 agaggatgtc gtagcgggcg gcgctttcag gcaaaggttc gccgttcgga agcagcgccg
     6541 cgagcttgag gccgccggtg ttgagcacga aggtttcctc gagcgagcgg ccgctctcga
     6601 ctttgatctt cttcgtcaga ttggaaaggc cgtaggcggc attgacgaga tattcgccgg
     6661 ggagcagcgc cgccgtcggc atcggatcgc ggtgcgtgct gacgagtttg tagccaacgc
     6721 ctcccggcaa cgacttcgac tcgtagacac gccaggtgag ccccgattgc agccgcggcc
     6781 ccgcttccga gacgagagcc ttgaaaatga cggggacggg aagctgcacc tgtgccgcca
     6841 tcgattgcgt ggaaggcttc ggtgcgacgg gtgcggatgg agctcctcct tctggcgcgg
     6901 atggagcgag cgccatcttg gcgggaggac gc
//
LOCUS       DASUQP010000077        13339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1534152, whole genome shotgun sequence.
ACCESSION   DASUQP010000077 DASUQP010000000
VERSION     DASUQP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534152"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFY74_03470"
     CDS             <1..385
                     /locus_tag="VFY74_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5930801.1"
                     /translation="VRLAGNFLNEDGFASLEYAVKFLGAPLILVLGHTNCGAIDAAIK
                     VVKERIELPGHLPELIKSIEPAVIAAHGRHPSDLLAATTEENVRLNVNRLLDDAPILT
                     EAVNAKKLAVAGGIYDLATGKVNLI"
     gene            410..1432
                     /locus_tag="VFY74_03475"
     CDS             410..1432
                     /locus_tag="VFY74_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX5930802.1"
                     /translation="MAGSLERWRAEKTAAYLSRAVARRERDPAKGALFQKMAEAAEQQ
                     AGILAKDLGGVPRFHPSLRSRITAFLIGIFGARATRHVLSASKVRGVSVYRGKAAPQA
                     HPMPTSVEDVGRRHKSFGGGTLRAGVFGVNDGLVSNTCLVMGVAGAAVEPNVLILTGV
                     AGLLAGAFSMAAGEFVSMLSQKEMFEHQIAQEKDELERYPKEEAEELALIFAARGIPL
                     DEARDVATKLIANPEKALDTLAREELGLNPDDLGSPIGAAVSSFVMFSIGASLPLIPF
                     LLGLPRGIEIAAVISGVALFGVGAALSLFSGRSALLGGMRMLLIGSAAAAATYMIGSL
                     FDVSAV"
     gene            1456..2907
                     /locus_tag="VFY74_03480"
     CDS             1456..2907
                     /locus_tag="VFY74_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX5930803.1"
                     /translation="MLFGDILERAKRLLNLGPTLAPAPIRTARAQGAIPPGPLPGSRL
                     TPDFVRAIGRFAYLWGWPLVNIYNRHWTQGWVKTQTFLVGGVAPIAPINHLAMLTGYN
                     EPGQRYITCPSQDLIYGFGVLDLSREAVVVQVPDFGERFFVFQATDQRTDAFSAMGSM
                     YGTKPGFYLLAGPDWKGRAPSGINATFRASTNLGSIIPRVFQADDAADNAAVQPLLRK
                     IMSYPLSKFDGTMKEKDWSDILPLPWKKLDDEEWRWVKPASFFDTLPKVLDLCPPLPG
                     EEALYALLRSVLAAADEDRTLRETLNEAAAEADEVLVAPLLEFRNFGVVLAHHWTTVL
                     NSAAFGTDYYTRTAVAKSNIFINRPRETRYFYQDFDQNGARLDGSKRYTVTFAALPPV
                     KGFWSLTLYDQFHFLAPNALNRFSLGTKSRGLKFEADGSLVIHVQKERPEPDKVPNWL
                     PAPDGPFSLYIRAYWPLKPIAEGRWTPPAVVPV"
     gene            complement(2904..4208)
                     /locus_tag="VFY74_03485"
     CDS             complement(2904..4208)
                     /locus_tag="VFY74_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5930804.1"
                     /translation="MGADAARQSAIAKTVRRLIPFLVLCYAVNFLDRVNVGYAALAMN
                     ADLGFTPSIYGIGAGIFFVGYILFEIPSNLALKRFGARIWIARIMISWGLIATAMALV
                     SGERSFYVMRFLLGVAEAGFFPGIVLYLTYWFPARERARIVALFMAAVPIATMVGGPV
                     SGALLELHGFLGLKGWQWLFIIEGVPAILLGAIALRFLDDTPEQASWLGAEERQALSR
                     TLAAEADAARAIGYAELGQALTRPRVLALGLLYFCIVMGLYGLGFWMPQVIQTYGLGP
                     LAIGFATAIPYFFAAVAMVAWGAHSDRTGERIWHIALPLFVGGAAFAWSAAPLSLALM
                     LVALTLATSGTYAAIGTFWSLPTAILTGTGAAAGLALVNSLGNVSGLVGPPIIGALKQ
                     GTGAFTASLLFLAGMLILGAAIVLAFGAVERARAARSRLAAR"
     gene            4367..4939
                     /locus_tag="VFY74_03490"
     CDS             4367..4939
                     /locus_tag="VFY74_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX5930805.1"
                     /translation="MAIPLDAAAAAMREAMRETVKRYSLWYLLQGVLMVLAGVLALIY
                     PLLASVAMVYLLGWVLIVSGVLQAIGLIGARDVPHYWLALISVVLAIVIGVLLLRQPE
                     SGLLLMTVLLIVFFMVEGISKIIFALAIRPFPHWGWVLASGLVGIVLAVYLWANMPVS
                     AEWVLGFLLGIQLICEGAALAYLAWRVRTG"
     gene            complement(4983..6479)
                     /locus_tag="VFY74_03495"
     CDS             complement(4983..6479)
                     /locus_tag="VFY74_03495"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5930806.1"
                     /translation="MLSPSDIVVRSTNLTERQAAQSSGGSPATPEPTPTTMAERFAPE
                     AQKGLADDPVRPVVEPGVMPGFIVDRARARVSSTGQWLLVAAMLVSLIPTAVILALLW
                     KGVIAIPGESEAQKLPDRAPLAASEQAALVPAPVLQVAPEPEIVVAPKPEIAPKAEIA
                     LTAPSAIAAKSGEEVPFTIAIDSAEALPARSVIAIRAMPEGATFSQGRPYGTSEWNLT
                     PEEIGDLKLRLPETASGAADISVALMAADGTILANATTRLDIAPDPNKALILRPDEAG
                     RIADLIAHGHAMIDVGYFPGARAYFKRAAEAGSGDAALLLGATFDAEFIDKIGAHGIK
                     PDPREARGWYERARKLGVEDIDTKLKALKEDVTRRLPAVQAIEATAAPAEAPPPAPAE
                     PTPAAPAATPASQPTSAETEREVDPPPGATETSLPPGSEEWVEITNYANLRAEPSGTA
                     ETLRVAEKGAKLRVTGRKGNWVQVTDPATSEVGWVYGRYIETAQAPQH"
     gene            complement(6510..7658)
                     /locus_tag="VFY74_03500"
     CDS             complement(6510..7658)
                     /locus_tag="VFY74_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5930807.1"
                     /translation="MRIALACGLCLIAVVSVAAPSARDKANAEPNMLAAQNVRENFRA
                     RLNENVVTIMAGSPSGTDLAIVQDLADVLEDGDNLRVLPMVGKGPEHNIKDVMFLRNV
                     DMGVTQANLLKHFDRTGELGPNLKERIAYIAKLFNEEMHILVRSNVNDISELNGRYVN
                     FGAPGSGGAITGRLIFAALGVDAQEVNLNDKDAIEKLKAGDIDATIVVTGKPAPVFAN
                     LKDTAGLKLLGVPYAKTLEDEYYPATFTHDDYPALVPEDGRVDTVSVCAVLVSFNWPS
                     DSVRYKKIAKFVDRFFAKFDSFLVAPRHPKWREVNFAATLEGWRRSTLAQNWIDRAQA
                     KPMDSAARSNFDAFLAQASSTRGTPISDAERADLFRAFLEWNRGRKQN"
     gene            complement(8133..8507)
                     /locus_tag="VFY74_03505"
     CDS             complement(8133..8507)
                     /locus_tag="VFY74_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930808.1"
                     /translation="MRHVLMVSLIVLSAAFMPALAAASLQGSWRGSGVVTHRRGADSV
                     RCRVTFTKISAKSFAVSSQCTTKTGRYDVTGRVASSGANRYSGQLQGQGQGGRTVIVQ
                     NGNRLAVTVTSKRGSARLSLSR"
     gene            complement(8596..10413)
                     /gene="lepA"
                     /locus_tag="VFY74_03510"
     CDS             complement(8596..10413)
                     /gene="lepA"
                     /locus_tag="VFY74_03510"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5930809.1"
                     /translation="MTKFETTPLSRIRNFSIIAHIDHGKSTLADRLIQLCGGLSAREM
                     KEQVLDSMELERERGITIKAQTVRLDYRARDGALYELNLIDTPGHVDFAYEVNRSLAA
                     CEGSLLVVDATQGVEAQTLANVYQALDNDHEVVPVLNKIDLPAAEPGRVRQQIEDVIG
                     LDAHDALEISAKTGKGVEEVLEAIVQRLPHPKGDARAPLKALLIDSWYDSYLGVVVLV
                     RIFDGVLHKGQRIRLMSTGAAYQIDRAGIFRPKQEMRDRLGPGEIGFFTASIRQVADT
                     RVGDTVTDERKPADVPLSGFKPAQPVVFCGLFPVDASEFEDLREAVAKLRLNDASFSY
                     EMETSAALGFGFRCGFLGLLHLEIIRERLEREFDIDLIATAPSVIYRVHLTDGSMKEL
                     HNPADMPEPQKIAKIEEPWIEATILVPDEHLGAVLKLCEDRRGRQKNLSYAGARAMLV
                     YDLPLNEVVFDFYDRLKSVSRGYASFDYRIIGYEEGDLVKMALLVNNEPVDALSVIVN
                     RSRAETRGRAMVEKLKDLIPRHLFKIPIQAAIGGKVIARETISALRKDVTAKCYGGDI
                     TRKRKLLDKQKAGKKKMRSFGRVEIPQEAFIAALKMDAD"
     gene            10556..10759
                     /locus_tag="VFY74_03515"
     CDS             10556..10759
                     /locus_tag="VFY74_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930810.1"
                     /translation="MLKKVLFAVIATAFVAATALPIQVAPAAAAEGMSCKQAAKAKFP
                     GDRKARHAYKKECKAAYKASQKA"
     gene            10998..11207
                     /locus_tag="VFY74_03520"
     CDS             10998..11207
                     /locus_tag="VFY74_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930811.1"
                     /translation="MLKKVFLAAAVSLLLVGVTLTAAPTEVYAGKGHCLKKAKAKYAD
                     DRKARRAYRKACHAHHKAWKKAHKK"
     gene            complement(11261..12043)
                     /locus_tag="VFY74_03525"
     CDS             complement(11261..12043)
                     /locus_tag="VFY74_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX5930812.1"
                     /translation="MSGARKLDRRGFLARGLAAASAALLGGCEELSDQTWVKRALESG
                     EALTRVTQEALLSPQTLAREYGEADLSPEFRANGSTNPDDPAYVAHAKNGFADWKLSI
                     GGLVEAPLDLSLAELRAMPSRTQITRHDCVEGWSCIGKWTGVKLGALFERVRLKADAR
                     YIVFHCADMLGSTGTEADRYYESIGLADAFHEQTILAYEMNGQTLPIAHGAPLRLRVE
                     RQLGYKMAKYVMRIEAVESLDGIRGGRGGFWEDLGYEWYAGI"
     gene            complement(12040..12879)
                     /locus_tag="VFY74_03530"
     CDS             complement(12040..12879)
                     /locus_tag="VFY74_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5930813.1"
                     /translation="MEAEETNPKRKGEVIYRHSATVRMTHWVNALVLLVLLMSGLQIF
                     NAHPALYLGDKSDFANPILAMGTVEEGERLTGVTTIFGFAIPTHGVLGLSGDAESGYD
                     ERGFPWWSTLPGDRDLAMGRRWHFFFAWLFLLNGLAYLLWSLASGHLRRDLTPSRNEL
                     KHIGASILEHARPKFPKGDEAKRYNVLQKLAYLAVALVLLPLMLLTGLAMSPAMDAAF
                     PVLLDMFGGRQSARTIHFISATGIVLFVVVHLVMVLISGVWNNLRSMITGRYVIEPAE
                     GKS"
     gene            complement(12941..>13339)
                     /gene="msrA"
                     /locus_tag="VFY74_03535"
     CDS             complement(12941..>13339)
                     /gene="msrA"
                     /locus_tag="VFY74_03535"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5930814.1"
                     /translation="PNPNYGDHEGYVEAVKVTYDPAKVTYATLLDHFWRNIDPFDPNG
                     QFCDQGDAYRSVVFVGDDEEKTLAETTKTEIAERYKKPVATAIKPVTTFTDAEDYHQD
                     YHNQNPVSYKVYKWGCGRAQKLEELWGKPS"
BASE COUNT         2338 a         4277 c         4309 g         2415 t
ORIGIN      
        1 cgtgcgcctc gccgggaatt tcctcaacga agacggcttc gccagcctcg aatacgcggt
       61 caagttcttg ggcgcgccgc tcatcctggt ccttgggcac accaattgcg gcgcgattga
      121 cgcggcgatc aaggtggtga aggaacggat cgagctgccg ggccatcttc cggagctgat
      181 caaatccatc gagcccgcgg tgattgccgc gcatggccgc catccaagcg acttgcttgc
      241 cgcgaccacg gaggagaatg tgcgcctcaa tgtgaaccgg ctgctcgacg acgccccgat
      301 cctgaccgaa gccgtgaatg cgaagaagct cgcggttgcc ggcggcattt acgatctcgc
      361 gaccggcaag gtgaacttga tctgagtgaa ctcgggctgg tggcgctcaa tggcagggag
      421 tcttgagcgg tggcgcgcgg aaaagaccgc cgcctatctt tcgcgtgcgg ttgcgcgcag
      481 ggagcgtgac ccggccaagg gcgcgctgtt ccagaagatg gccgaagcgg cggagcagca
      541 ggccggaatt cttgccaaag atctcggcgg ggttccgcgc tttcacccct cgctcaggtc
      601 gcgcatcacc gcctttctca tcggcatctt cggtgcacgc gccacgcgcc acgtgttgtc
      661 ggcttcgaag gtgcgcgggg tctcggtcta tcgcggcaag gccgcgccgc aagcgcatcc
      721 catgcccacc tccgtcgagg atgtcggccg gcgtcataaa tccttcggcg gcggcacgct
      781 gagggccggc gtattcggcg tcaatgacgg gctcgtctcg aatacgtgtc tggtcatggg
      841 cgttgcgggc gccgcggtcg agccgaacgt tctgatcctc accggcgtgg ccgggcttct
      901 cgccggggcc ttttccatgg cggccggcga attcgtctcc atgctgtcgc agaaggagat
      961 gttcgagcat cagatcgcgc aagagaaaga cgagctcgag cgctatccca aggaagaagc
     1021 tgaggagctt gcgctgatct tcgccgcgcg cggcattccg ctcgacgagg cgcgcgatgt
     1081 cgccacgaag ctgatcgcaa accccgagaa ggcgctcgac acgctcgccc gcgaggagct
     1141 tggcctcaat cccgacgatc tcggctcgcc gatcggcgcg gccgtctcct ccttcgtcat
     1201 gttctcgatc ggcgcgagcc tgccgctcat tcctttcctg ctcgggcttc ctcgcggaat
     1261 tgaaatcgcg gccgtaatct cgggcgtggc gctgttcggg gtcggcgcgg ccttgagcct
     1321 gttctccggg cgaagcgcgc ttcttggcgg tatgcggatg ttgctgattg gaagtgcggc
     1381 ggcggccgcg acctatatga tcggttcgtt gttcgacgtc tcggcggttt gatcgaaagc
     1441 ggacgcgcgc gccccatgct gttcggcgac attcttgaac gcgccaagcg tctgctcaac
     1501 ctgggtccga ccctcgcacc cgccccgatc aggaccgccc gcgcgcaagg ggcgataccg
     1561 cccggtccct tgcccggctc aagactcacg cccgatttcg tccgcgcaat cggccgcttc
     1621 gcctatctct ggggctggcc gctcgtgaac atctacaacc gccattggac gcaaggctgg
     1681 gtcaagaccc agactttcct cgtcggcggc gtcgctccca tcgcgccgat caatcacctc
     1741 gccatgctga caggctataa cgagcccggc cagcgctaca tcacctgccc gagccaggac
     1801 ctcatctacg gcttcggcgt cctggatctg tcccgcgagg ccgtggtcgt ccaggtgccg
     1861 gatttcggcg agcgcttttt cgtcttccag gccaccgatc aaaggaccga cgccttcagc
     1921 gcgatggggt cgatgtacgg gacgaagccc ggcttctatc tgctggccgg ccccgactgg
     1981 aagggcaggg cgccctccgg catcaacgcc acgttccgcg cctcgaccaa tctcggctcg
     2041 atcattccgc gcgtgttcca ggccgacgac gctgccgaca acgccgcggt ccagccgctg
     2101 ctccgcaaga tcatgtccta tccgctgtcg aagttcgacg gcacgatgaa ggagaaggac
     2161 tggagcgaca tcctgcccct tccctggaaa aaactcgacg acgaggaatg gcggtgggtg
     2221 aagcccgcga gtttcttcga tacgctgccc aaggtgctcg atctctgccc gccgctgcca
     2281 ggcgaagaag cgctctatgc gctcctccgt tccgttcttg cagccgcgga cgaggaccgg
     2341 actttgcgcg aaacactgaa tgaggcggcg gctgaagccg atgaggtgct ggtcgcgccg
     2401 ctgctcgagt ttcgcaattt tggcgtggtc ctcgcccatc actggaccac ggtcctgaac
     2461 agcgccgcgt tcggcaccga ttattacacg cgcaccgcgg tggcgaagtc gaacatcttc
     2521 atcaaccggc cgcgcgagac ccgctatttc taccaggact tcgatcagaa cggcgcgagg
     2581 ctcgatggat cgaagcgcta caccgtgacc ttcgcggcgc tgcccccggt gaaaggcttc
     2641 tggtcgctca cgctctacga ccagttccac ttccttgcgc cgaacgcgct taaccgcttc
     2701 tcgctcggca ctaagagccg gggacttaag ttcgaggccg acggctctct cgtcatccat
     2761 gtgcaaaagg agcgccccga gcctgacaag gtgccgaact ggctgcccgc gcccgacggc
     2821 cccttttcgc tctacatccg cgcctactgg ccgcttaagc cgatcgcgga aggacgctgg
     2881 acgccgcccg ccgtcgtccc ggtctagcgg gccgcgaggc gggaccgcgc ggcgcgggcg
     2941 cgctccacag cgccgaaggc aagcacgatc gcggcgccga ggatgagcat gccggcgagg
     3001 aacaggaggc tcgcggtgaa cgcgcccgtc ccctgcttga gcgcgccgat gatcggaggg
     3061 ccaacgagcc cgctgacatt gccgagcgag ttgaccagcg caagtccggc cgcggcgccc
     3121 gtgccggtga ggatcgcggt ggggagcgac cagaacgtgc cgatggcggc gtaggtgccg
     3181 gacgtggcca gagtgagcgc gacaagcatg agcgcgagcg agagtggcgc ggccgaccag
     3241 gcgaaggccg cgccaccgac gaacagcggc agcgcgatat gccatatgcg ttcgccggtc
     3301 cggtcggaat gcgcgcccca cgcgaccatg gcgacggcgg cgaagaaata ggggatcgcg
     3361 gtggcaaaac cgatcgcgag cggaccgaga ccgtaggtct ggattacctg cggcatccag
     3421 aagccgaggc catagagccc catcacgatg cagaaataga ggagaccgag cgcaagcacg
     3481 cgggggcgtg tcagcgcctg gccgagttcg gcatagccta tcgcccgcgc cgcgtccgcc
     3541 tcggccgcaa gcgtgcgcga gagcgcctgc cgctcctccg cgccgagcca gctcgcctgt
     3601 tcgggcgtgt cgtcgagaaa acgaagcgcg atcgcgccga gcaagatcgc cggcacgcct
     3661 tcgatgatga acagccactg ccaacccttg agcccgagga agccgtgcag ctcgagcagc
     3721 gcccccgaga ccgggcctcc gaccatcgtc gcgatcggca ccgccgccat gaacagcgcc
     3781 acgatgcgcg cccgctcccg cgccgggaac caataggtga gataaagcac gatcccgggg
     3841 aagaagcccg cttcggccac gcccaggaga aagcgcatga cgtagaagct cctctccccg
     3901 ctcacgaggg ccatggcggt cgcgatcagc ccccagctga tcatgatgcg ggcgatccag
     3961 atgcgggcgc cgaagcgttt cagcgcgagg ttgctcggga tctcgaacag gatgtagccg
     4021 acgaagaaga tgccggcgcc gatgccgtag atcgagggcg taaagccgag atccgcgttc
     4081 atggcgagtg cggcgtagcc gacattcacc cggtcgagga aattcacggc gtagcagaga
     4141 acgagaaagg gaatgaggcg gcgcaccgtc ttggcgatcg ccgactgccg tgccgcgtca
     4201 gcacccaccc gcgcccctcc ctcgacccaa gagccgtgct gcgtccgccc tgaaatgaac
     4261 gagccgctcc catccggcaa gggcgaaaag ggagggctcg ccagccttca gagcgccgat
     4321 ggagtatcct ccgagcgcaa actgattcgc cttgaggagg agaaccatgg ctattccact
     4381 cgatgccgcc gccgcggcca tgcgcgaggc catgcgcgag acggtgaagc gctattcgct
     4441 ctggtacttg ctgcaaggcg tcttgatggt gctcgcaggc gtgcttgcgc tcatctatcc
     4501 cctgctcgcc tccgtcgcca tggtctatct gctcggctgg gtgctgatcg tcagcggcgt
     4561 gcttcaggcc atcgggctga tcggcgcccg cgacgtgccg cattactggc tggcgctgat
     4621 ctcggtcgtg ctcgccatcg tcatcggcgt gctacttctc cgccagcccg agagcgggct
     4681 tctgctcatg accgtcctcc tgatcgtgtt cttcatggtc gaggggatct ccaagatcat
     4741 cttcgcgctc gccatcaggc ccttccccca ttggggatgg gtgctcgcga gcgggctcgt
     4801 cggcatcgtg cttgcggtct atctgtgggc caacatgccg gtgagcgccg aatgggtgct
     4861 cggcttcctg ctcggcatcc agctcatctg cgagggcgca gcgctcgcct atctcgcctg
     4921 gcgggttcgc accggctgag gctcagccag cgcggggcgc gcgtccctgg gcgttgcggc
     4981 gactagtgct gaggcgcctg agccgtttcg atatagcgac cgtaaaccca gccgacctcc
     5041 gaggtggcgg gatcggtcac ctgcacccag ttccctttgc ggccggtcac gcggagcttt
     5101 gcgcccttct ccgccacccg caacgtttcg gcggttccag acggctcggc gcgaagattg
     5161 gcgtagttcg tgatctcgac ccattcttcg ctgcccgggg gaagcgatgt ctccgtggcg
     5221 ccgggaggcg gatccacctc cctctccgtc tctgcggagg ttggctggct cgcgggcgtg
     5281 gcggctgggg cggcaggcgt cggctccgca ggcgcgggcg gcggcgcctc ggccggtgcc
     5341 gcggtcgcct cgatcgcctg aacggcgggg aggcggcggg tcacgtcctc cttgagcgcc
     5401 ttgagcttcg tgtcgatatc ctcgacgccg agctttcttg cacgctcata ccagcctcgc
     5461 gcctcgcgcg ggtctggctt gatgccatga gcgccgatct tgtcgatgaa ctccgcatcg
     5521 aaagtggcgc cgagcagaag cgcggcatcg ccgcttccgg cctccgccgc gcgcttgaaa
     5581 taggcgcgcg cgcccgggaa atagccgaca tcgatcatcg cgtggccgtg agcgatcaga
     5641 tcggcgatgc gtccggcctc gtcgggccgg aggatcagag ccttgttcgg atcgggcgcg
     5701 atgtcgagcc tggtcgtggc gttggcaagg atcgtgccgt cggccgccat caacgcgacg
     5761 ctgatgtcgg cggcaccgct cgccgtctcc ggcagcctga gcttcaggtc gccgatctcc
     5821 tcgggcgtca agttccactc agaggtgcca tagggacgcc cttgcgagaa ggtggcgccc
     5881 tcgggcatgg cgcggatggc gatgacgctg cgcgcgggca gcgcctcggc cgaatcgatg
     5941 gcgatggtga agggaacctc ctcgcccgac ttcgccgcga tcgcgctcgg ggcggtgagc
     6001 gcgatctcgg ccttgggcgc gatctcgggc ttgggcgcga cgacgatctc cggttcgggc
     6061 gcgacctgga ggacgggcgc cggcacgagc gcggcctgct cgctcgcggc gaggggggcc
     6121 cgatcgggaa gcttttgcgc ctcgctctcg ccggggatcg cgatgacgcc cttccacaga
     6181 agcgcgagga tcaccgccgt cgggatcaag gagacgagca tggcggcaac cagaagccat
     6241 tgtccggttg aggaaacccg ggcgcgagcg cgatcgacaa tgaatccggg catgaccccg
     6301 ggctcgacca ccggccggac cggatcgtcg gccagaccct tctgcgcttc gggagcgaag
     6361 cgctccgcca ttgtggttgg cgtcggctca ggagttgctg gcgacccgcc tgaactttgg
     6421 gccgcctgcc gctcggtcag gttggtcgac ctgacgacga tgtcggacgg actgagcatg
     6481 gaaccctcct acacattaca aaaagagagt cagttctgtt tgcgccccct attccactcc
     6541 aggaacgcgc ggaacagatc ggcgcgctcc gcatcggaga tcggcgttcc gcgcgtgctg
     6601 ctcgcctggg cgagaaaggc gtcgaaattg ctgcgcgcgg cgctgtccat tggcttggcc
     6661 tgggcgcggt cgatccagtt ctgcgccagt gtcgaacgcc gccagccttc gagcgtcgcc
     6721 gcaaaattga cctcgcgcca ttttggatga cgcggcgcga caaggaagga atcgaacttc
     6781 gcgaagaagc gatcgacgaa cttggcgatc ttcttgtagc ggacggaatc gctcggccaa
     6841 ttgaacgaca ccagcacggc gcagaccgag acggtatcga cccgcccgtc ctcggggacg
     6901 agcgcgggat agtcgtcatg ggtgaacgtc gcggggtaat actcgtcttc gagcgtcttg
     6961 gcataaggca cgccaagaag cttgagtccc gcggtgtctt tgaggttggc gaagaccggc
     7021 gcgggcttcc ccgtcaccac gatagtggcg tcgatatcgc ccgcctttag cttttcgatc
     7081 gcatccttgt cgttgaggtt cacctcttgc gcatcgacgc cgagggccgc gaagatcaat
     7141 cgcccggtga tcgcgccgcc gctgcccggc gctccgaaat tcacatatct gccgttcagc
     7201 tcgctgatgt cgttcacgtt ggagcgcacg agaatatgca tctcctcgtt gaacagcttg
     7261 gcgatgtagg cgatccgctc cttcaggttc ggcccgagct cgccggtgcg gtcgaaatgc
     7321 ttgagcagat tggcttgcgt cacgcccata tcgacgttgc gcaggaacat cacgtccttg
     7381 atgttgtgct cgggtccttt gccgaccatg gggagcacgc gcagattgtc gccgtcttcg
     7441 agcacgtcgg cgagatcctg gacgatggcg aggtcggttc cgctcggact tccggccatg
     7501 atcgtcacca cgttctcgtt caggcgcgcc cggaaattct cgcgcacgtt ctgagcggcg
     7561 agcatgttgg gctcggcgtt tgccttgtcc ctggcgctcg gcgcggcaac gctaacaacg
     7621 gcgatcaggc aaagtccgca ggcaagggcg atacgcatga caagcccctc ttcttcgcgc
     7681 gctgatgttg acgccggcac gcctccgcct tggcgcgcat gcagggtggc cggttacgct
     7741 gggatgtctg tccgtcggga aaaaggggcg tggcctaggt cagcaccaca tttcgccgat
     7801 cgtgctgcga tccctcctgg ccaaaccaga tgtgatattt taactatagt caagtagtca
     7861 tcggccgtaa gcacgaaaat aacactttct cgccgcagtc tcgacagaaa gtaacttgaa
     7921 agcggccaga ctatggccaa gaaatatctt ggccaaagca acgaaaactt tgccgttaca
     7981 gcaactaagc tgtatcgatt gatatatcgt attgcgcagc caaaagtcgc gccgactgga
     8041 cgcaacgagt tcgcatctaa cgccggcggc tgattggagt tccccggggc gcggaaaaag
     8101 ggagcgttta tcgctcggcg gcggcgaggc cgctagcgcg acaagctcag tctggcggaa
     8161 cctctcttgc tcgtcactgt gaccgcgagg cgattgccgt tctgcacgat gacggttctg
     8221 ccgccttggc cctggccctg gagctggccg gagtagcgat tggcgccgga actggcgacg
     8281 cgtcctgtca cgtcgtagcg accggtcttg gtcgtgcatt gcgaagaaac cgcgaaggac
     8341 ttcgcgctta tttttgtgaa ggtcacccgg catcgcaccg aatcggcgcc gcggcgatgg
     8401 gtgacgacac ccgagcctcg ccacgacccc tgaagcgaag ccgccgcaag cgcaggcatg
     8461 aacgcggcgc tcaagacgat cagagaaacc atgagaacat ggcgcatgag acctcccctc
     8521 aacgcttgga ttgttgaagc gaaaaacgcg cgacggacct tcttgggtcc atcattcgcc
     8581 cttttcgagt caagcctagt ccgcgtccat cttcagcgcg gcgatgaagg cttcctgcgg
     8641 gatctcgacg cggccgaagc tccgcatctt tttcttcccg gccttctgct tgtcgagcag
     8701 cttgcgcttg cgcgtgatgt cgccgccata gcacttcgcg gtcacgtcct tgcgcagcgc
     8761 gctgatcgtc tcgcgcgcga tcacctttcc gccgatcgcc gcctggatcg gaatcttgaa
     8821 caggtgccgc gggatcaggt ccttgagttt ctccaccatg gcgcggcccc gcgtttcggc
     8881 gcgggaccgg ttgacgatca ccgacagcgc gtcgaccggc tcgttattga cgagcagcgc
     8941 catcttcacg aggtcgccct cctcgtagcc gatgatgcga taatcgaacg aggcgtagcc
     9001 gcgcgagacg gatttgagcc ggtcgtagaa atcgaacacg acttcgttga gcggcagatc
     9061 gtacaccagc atggcccgcg cgccggcata gctcaggttt ttctgccggc cgcgccggtc
     9121 ctcgcacagc ttgagcacgg cgccgagatg ctcgtccgga acgaggatgg tggcctcgat
     9181 ccacggctcc tcgatcttcg cgatcttctg cggctcgggc atgtcggcgg ggttgtgcag
     9241 ctccttcatc gagccgtcgg tcaggtgcac gcggtagatc accgaaggcg cggtggcgat
     9301 gagatcgata tcgaattcgc gctcgagcct ttcgcggatg atctcgagat ggagcaggcc
     9361 gagaaagcca cagcggaagc cgaagccgag cgcggccgat gtctccattt catagctgaa
     9421 gctcgcatcg ttgaggcgga gcttggccac ggcctcgcgc aggtcctcga actcgctggc
     9481 atcgacggga aagaggccgc agaacacgac gggctgcgcc ggcttgaagc cggaaagcgg
     9541 cacatccgca ggcttcctct cgtcggtgac agtgtcgccc acgcgcgtgt cggcaacctg
     9601 ccggatcgaa gcggtgaaga agccgatctc gcccggcccg agccggtcgc gcatctcctg
     9661 cttggggcgg aagatgccgg cgcgatcgat ctggtaggcg gcgcccgtcg acatcagcct
     9721 gatgcgctgg cctttgtgca gcacgccgtc gaagatgcgc accagcacca ccacgccgag
     9781 ataggaatcg taccagctgt cgatcagaag cgccttgagc ggcgcgcgcg cgtcgccctt
     9841 ggggtgcggc aggcgctgca cgatcgcttc cagcacctcc tcgacgccct tgccggtctt
     9901 cgccgaaatt tccagcgcat catgcgcgtc gagcccgatc acgtcctcga tctgctggcg
     9961 gacgcggccg ggctcggcgg cgggcaagtc gatcttgttg aggaccggca ccacttcgtg
    10021 atcgttgtcg agcgcctgat agacattggc gagcgtctgc gcctcgaccc cttgggtggc
    10081 gtcgaccacg agcagagagc cctcgcaggc cgcaagactc cggttcacct cataggcgaa
    10141 gtcgacatgg cccggcgtgt cgatcaggtt cagctcgtag agcgcgccgt cgcgcgcgcg
    10201 ataatcgaga cgcacggtct gcgccttgat ggtgatgccg cgctcgcgct ccagttccat
    10261 cgagtcgagc acctgctcct tcatctcccg cgcgctcaag ccgccgcaga gctggatcag
    10321 ccggtctgcc agcgtcgatt tgccgtggtc gatatgagcg atgatggaga agttgcggat
    10381 gcgggaaagc ggcgtcgtct cgaatttcgt catgcgcggc ttggtacggg agcgggaccc
    10441 gaagggtcaa gcaagcgttc agcaaatgcg gcgaaaaggt gagacgaagt gccctattgt
    10501 ggtagcggtt caggtttaga gagtgccgct tccgcaaacc aacggagata aactgatgct
    10561 gaaaaaggtg ttgtttgctg ttatcgccac cgcttttgtt gccgccactg cgctcccgat
    10621 ccaggtggcg ccggccgcgg ctgccgaggg aatgagctgc aagcaggcgg caaaggcgaa
    10681 attcccgggc gacagaaaag cccggcacgc ctacaagaag gagtgcaagg ctgcctataa
    10741 ggcctcgcaa aaggcttaaa gattagggtc tcgcgacccg actacgcttc agagaaaggg
    10801 gcccaggcgg cccctttctt aatgccttga acttgcatgg cccgacgaaa tgcccggttt
    10861 gggcgctacg agccaaccaa aaaccccaag aatgttagcc aaatcggcag atcggggacg
    10921 aagccggttt acgaaggcgt ccaaaacggc ccttactccg ccgcgtaagt cacgtcgtca
    10981 cgcacgggag taaccttatg cttaagaaag tgtttttggc tgcagcggtg tccctcctct
    11041 tggtcggcgt caccctgacc gccgcgccca ccgaagtgta tgccgggaag ggccattgct
    11101 tgaagaaggc gaaggcgaaa tacgccgacg accggaaggc gcgccgcgcc tacaggaagg
    11161 cctgccacgc gcatcacaag gcctggaaga aggcccacaa gaagtaggct cgctttgcga
    11221 ttaagggacc cgcctcggcg ggtcccttta ttttgccggg tcatatgccg gcgtaccact
    11281 cgtagccgag atcctcccag aaaccgccgc ggccgcccct gatgccgtcg aggctttcga
    11341 ccgcctcgat ccgcatcacg tatttcgcca tcttgtagcc gagctgccgc tcgacgcgga
    11401 gacgtagcgg ggcgccgtgc gcgatgggca gcgtctggcc gttcatctcg taggcgagaa
    11461 tcgtttgctc gtgaaaggcg tcggcaagcc cgatcgactc gtaatagcgg tcggcctcgg
    11521 tgccggtgct tccgagcata tcggcgcaat ggaaaacgat atagcgcgcg tccgccttga
    11581 gccggacgcg ttcgaacagg gcgccgagct tcacgcccgt ccacttgccg atgcagctcc
    11641 acccctcgac gcagtcgtgc cgggtgatct gggtgcggga gggcatggcg cgaagctcgg
    11701 cgagcgaaag atcgagcggc gcctcgacga gaccgccgat cgacagcttc cagtctgcga
    11761 atccgttctt cgcatgggcg acataggccg gatcgtcggg attggtcgag ccgttcgccc
    11821 tgaactcggg tgagagatcg gcctcgccat attcgcgcgc gagcgtctgc ggggagagca
    11881 aggcttcctg cgtgacgcgg gtgagggcct cgccggactc cagcgcgcgc ttgacccaag
    11941 tctggtcgga cagctcctcg cacccgccga ggagtgccgc gctcgcggcc gccaaccccc
    12001 gcgcgagaaa tccgcgccgg tcgagcttcc gcgccccgct catgacttgc cctccgcggg
    12061 ctcgatcacg tagcggccgg tgatcatgga acgaagattg ttccagacgc cggagatgag
    12121 gaccatgacg aggtgcacga cgacgaagag cacgatgccg gttgcgctga tgaaatggat
    12181 ggtgcgggcc gactgcctgc cgccgaacat gtcgagcaag acgggaaagg ccgcgtccat
    12241 cgcgggcgac atggcgagtc ccgtcagcag catcaggggc agcagcacga gcgccacggc
    12301 gagataggca agcttctgca gcacgttgta gcgcttggcc tcgtccccct tgggaaattt
    12361 gggccgcgca tgttcgagga tggaggcccc gatgtgcttt agctcgttgc gcgatggcgt
    12421 gagatcgcgc cgaagatgtc cgcttgcgag gctccacaag agataggcga gcccgttcag
    12481 gaggaacagc caggcgaaga agaaatgcca gcgccgcccc atggcgaggt cgcgatcgcc
    12541 gggcagcgtc gaccaccacg gaaagccgcg ctcgtcatat ccgctttcgg catccccgct
    12601 caggccgagc acgccgtgcg tcgggatggc gaagccgaag atcgtggtga cgcccgtaag
    12661 ccgctcgccc tcctcgacag ttcccatcgc caggatcggg ttcgcgaaat ccgatttgtc
    12721 gccgagatag agtgccggat gggcgttgaa aatctgcaag ccgctcatca acagcacgag
    12781 cagcacgagc gcgttcaccc aatgcgtcat ccgcaccgtc gcagaatggc ggtagatgac
    12841 ctcgccttta cgcttcggat tggtctcttc ggcttccatc gccgtgccga tcctctgggg
    12901 ttcaaaacga gctacgcgtc gcgcggcgat ctggatgcac tcagctcggc ttgccccaaa
    12961 gctcctcgag cttttgcgcg cgcccgcagc cccatttgta aaccttataa ctcaccggat
    13021 tctggttgtg ataatcctgg tgatagtctt ccgcgtcggt gaaggtcgtc accggcttga
    13081 tcgcggtcgc gaccggtttc ttgtagcgct cggcgatctc cgtcttcgtc gtctcggcga
    13141 gcgtcttctc ctcatcgtcg ccgacgaaca ccacgctgcg ataggcgtcg ccctggtcgc
    13201 agaactgtcc gttggggtcg aacggatcga tgttgcgcca gaaatgatcg agcagcgtgg
    13261 cgtaggtcac cttggcgggg tcgtaggtga ccttgaccgc ctccacgtaa ccctcgtggt
    13321 cgccgtagtt gggattggg
//
LOCUS       DASUQP010000078        10049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_716782,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000078 DASUQP010000000
VERSION     DASUQP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716782"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFY74_03540"
     CDS             complement(<1..822)
                     /locus_tag="VFY74_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003586473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX5930815.1"
                     /translation="MRTDLFDFHLPEALIALRPVVPRDAARLLLVEPSATPQIADRMV
                     PDLPALLQPGDALVFNDTKVIPAELDGVRRRDGEGARVAVTLVERLGPDRWKALARPA
                     KRLKPGDRIVFGKGDETCLSCSLAATIAARGEEGEVTLVLDLAGAALDQAIHMLGNMP
                     LPPYIASRRTPDAQDLSDYQTVFAREEGAVAAPTAGLHFTDRLLRALADKGISEHFVT
                     LHVGPGTFLPVRSADTEGHRMHAEHGFVTPETAEALNAVKAEGGRIVAVGTTSLRL"
     gene            complement(835..1290)
                     /locus_tag="VFY74_03545"
     CDS             complement(835..1290)
                     /locus_tag="VFY74_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5930816.1"
                     /translation="MSLQEFSLTLIALLAGGLIALQAPINAEAASRLGHPLTAAAMSF
                     LVGTAALLFLTLLFARNHAQLEAFKSLPLYMLIGGGLLGAGYVTVNIMLTPRIGVAAL
                     MALGIAGQLFTALLIDRFGLFGLAERGLTMGRVSGALLVLAGALMVRAL"
     gene            complement(1308..1775)
                     /locus_tag="VFY74_03550"
     CDS             complement(1308..1775)
                     /locus_tag="VFY74_03550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5930817.1"
                     /translation="MANPEDTLIIETTKGRVTVAMRPDLAPNHVARIKELARQGFYDG
                     VVFHRVIEGFMAQTGDPTGTGTGGSGKKLGAEFSAEPHTRGAVSMARAQAPDSADSQF
                     FICFDDASFLNRHYTVWGRVIEGMENVDKINRGEPPVSPDKILSARIASDLTS"
     gene            complement(1797..2444)
                     /locus_tag="VFY74_03555"
     CDS             complement(1797..2444)
                     /locus_tag="VFY74_03555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5930818.1"
                     /translation="MRLQVSFAFAAALALALVAWTSGAGAVNLSDPATLNDKAPAVYK
                     AKFDTSKGAFVVEVHRDWAPNGADRFYNLVKNGFYDNARFFRVIDGFMVQFGINGDPG
                     IASVWRDANIKDDPVKQSNRRGTITFATAGPNTRTTQVFINFGDNAPLDGQGFSPFGK
                     VVSGMEVVDSLYKGYGEGAPHGNGPDQGRIQQQGNAYLEKDFPELDFIKTATIEK"
     gene            complement(2478..2972)
                     /gene="coaD"
                     /locus_tag="VFY74_03560"
     CDS             complement(2478..2972)
                     /gene="coaD"
                     /locus_tag="VFY74_03560"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5930819.1"
                     /translation="MRVGLYPGSFDPVTFGHVDIVRRAAHLVDRLVIAIGTHHDKHPL
                     FTAEERVKLTSVVLDPVAKKAGLKLDVTTYGNLTVDAAKAAGASLVIRGLRNAGDFDY
                     EMQMAGMNQALAPEIDTVFLASSPDARHIAASLVRQIAAMGGDVSSFVPPVVAAALKK
                     KFAR"
     gene            complement(3153..3551)
                     /locus_tag="VFY74_03565"
     CDS             complement(3153..3551)
                     /locus_tag="VFY74_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX5930820.1"
                     /translation="MDFPAGPDPAIPLRHYGQLQALCRRGGSYIDFFDTGTGANVIGA
                     TKVDIDDAWGFTLQAGVDVSLGNGWSLNADVKKTWLDTTVTWRNAAAVGGVNVVGDAD
                     VDPWIFSAGLGYRFNLGSIFGHGAEAAPIK"
     gene            complement(3527..3883)
                     /locus_tag="VFY74_03570"
                     /pseudo
     CDS             complement(3527..3883)
                     /locus_tag="VFY74_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(4244..4525)
                     /locus_tag="VFY74_03575"
     CDS             complement(4244..4525)
                     /locus_tag="VFY74_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930821.1"
                     /translation="MTAGSSAASVSTVLKRMRKTSAATGATSVASASGGAKLDRIKSP
                     GKRSAATCRVELGKAIFRTSAPKRTKNAISPFSPVSASACPRRGRKTVA"
     gene            complement(4869..7595)
                     /gene="gyrA"
                     /locus_tag="VFY74_03580"
     CDS             complement(4869..7595)
                     /gene="gyrA"
                     /locus_tag="VFY74_03580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5930822.1"
                     /translation="MADDDKPEVEGPAPSDIRPISLIDEMKRSYLDYAMSVIVSRALP
                     DVRDGLKPVHRRILYSMNENNYTPDRPYNKSARVTGDTMGKYHPHGNQAIYDALVRLA
                     QDFSMRLPIVDGQGNFGSVDGDPPAAERYTEVRLARPAMALLDDLENDTVDFQPNYDG
                     RETEPVVLPAKFPNLLVNGAGGIAVGMATNIPPHNLGEVTDACIAYLDNPAIGIDELV
                     EIVPGPDFPTGGLILGRSGIRAAYHKGRGSILMRGRVTTETVRKEREALIITEIPYQV
                     NKAAMVEKIAELVREKRIEGIADIRDESDRQGMRVVVELKRDAMADVVLNQLYRFSPL
                     QSSFGCNMVALIGGKPEQLTLKDMIRAFTEFREEVIGRRVKFLLRKARDRAHVLVGLA
                     IAVANIDEVIAVIRKAPDPAAARERLMARDWPAQDVVGLVELIADPRHKVSAKGTYRL
                     SEEQARAILDLRLQRLTALGRDEVGGELKTLGEQITDYLDILRSRARIVSIIKDELTA
                     IKNEFNTPRRTQILEMEGEVEDEDLIQREDMVVTVSHKGYIKRVPLSAYRAQRRGGKG
                     RAGMATREEDFVTRIFIANTHTPVLFFSTRGMAYKMTVWRLPVAAPQARGKALVNLLP
                     LDKDETITTILPLPEDVASWEKLDAMFATNYGSVRRNKLSDFIEVRQNGKIAMRLDSG
                     DRIIGVEVCGEEDDVLLTSADGQAIRFPVADVRVFKGRESPGVRGIKLERGDQVISMA
                     ILGHVEASPAERTAYVRQANSIRRGSADTEDVEAETNGQETVVLSPERYAELGAHEQF
                     VLTVSENGYGKRTSAYEYRISGRGGKGIIAMVVNQRNGKLIASFPVEETDQIMLVTDG
                     GQLIRVPVQDISVMGRSTQGVIVFSTGEGERVVSVEHISEENGGEEEAGEPA"
     gene            7984..8670
                     /locus_tag="VFY74_03585"
     CDS             7984..8670
                     /locus_tag="VFY74_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5930823.1"
                     /translation="MNRFLTTTAVGLFLGLAPVIAQDLPPANDAQTPPAASQPSQSPQ
                     VSPDPAQPSDPAAPIPGDSSQVSPSPMEPSDDAAPPAQSSQAPESIEPAAPAAPKSAE
                     ASDSPQFLSKQASDDWLASNLIGQSVYNAQDEMVGDINDLVTDHSGKIVAVLVGSGGF
                     LGLGEKDVALRFEDLRLAREDNDSIKIVADVSKETLAAAPDYETLAEQEVTVGSTKDD
                     REDIGGETTN"
     gene            complement(8897..9373)
                     /locus_tag="VFY74_03590"
     CDS             complement(8897..9373)
                     /locus_tag="VFY74_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX5930824.1"
                     /translation="MAGSVNKVILVGNLGADPEVRQTQDGRPIVNLRLATSENWRDKA
                     TGERREKTEWHRVVIFNENLARVAEQYLKKGSKVYIEGQLQTRKWQDQSGQDRYSTEV
                     VLQQYRGELTMLDGKPRDGTGESDQRNGDFGRSGPFAGGGGKGSSFDKELDDEIPF"
     gene            9630..>10049
                     /locus_tag="VFY74_03595"
     CDS             9630..>10049
                     /locus_tag="VFY74_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930825.1"
                     /translation="MQRTVDQRHATKRRPSERRHPRSQFLGFTPRQWPFIFVLIGNWL
                     FAAAFFVLAKFLWDWVPTEWTVGDRIALVIKDAVAAILPAVVAICIVAAQRLDPSMFV
                     GRTPKPNSAVDINNRFILNTVEQFILYFIGNAGLALYC"
BASE COUNT         1726 a         3202 c         3277 g         1844 t
ORIGIN      
        1 aaggcggagc gaggtggtgc cgacggcgac gatccgccct ccctcggcct tcaccgcatt
       61 cagggcttcc gcggtttcgg gcgtgacgaa accgtgttcc gcatgcatgc gatgtccctc
      121 ggtgtcggcc gacctgaccg gcaggaacgt gccgggcccg acgtgaagtg tcacgaaatg
      181 ctcggagatg cctttgtcgg cgagtgcgcg cagcagacga tcggtgaaat gcagcccggc
      241 ggtcggcgcg gcgacggcgc cttcctcacg cgcgaacacg gtctggtaat cgctcaaatc
      301 ttgcgcatcg ggggtgcggc gcgaggcgat atagggcggg aggggcatgt tgcccagcat
      361 atgaatcgcc tggtcgagcg cggcgccggc gaggtcgagg acgagtgtca cttcgccctc
      421 ttcgccgcgc gcggcgatgg tcgcggcgag gctgcacgag agacaagtct cgtctccttt
      481 cccgaagacg atgcgatcgc cgggtttcag ccgtttcgcc ggccgcgcca gagccttcca
      541 gcgatcgggc cctagccttt cgaccagcgt gaccgcgacc ctggcgcctt ccccatcgcg
      601 ccttcggact ccgtcaagct cggcggggat caccttggta tcgttgaaaa cgagcgcgtc
      661 gccgggctga agcagcgccg ggagatcggg gaccatccgg tccgcgattt gcggggtggc
      721 cgaaggctcg acgaggagga gccgcgcggc gtcgcgcggg accacggggc gaagcgcgat
      781 cagcgcttcc ggcagatgaa agtcgaacag atcggtgcgc accgattgga caggttagag
      841 cgcgcgcacc atgagcgcgc ctgcgagcac gaggagtgcg ccgctgacgc ggcccatggt
      901 cagaccccgt tcagccagcc cgaacaggcc aaaacggtcg atcaggagcg cggtaaacaa
      961 ctgcccggca atgccgagcg ccatgagcgc tgccacgccg atacggggcg tcagcatgat
     1021 gttgaccgtc acgtaacccg cgccgagcag gccgccgccg ataagcatgt aaagcggaag
     1081 cgatttgaag gcttcaagtt gcgcgtggtt gcgggcgaag aggagcgtga gaaacaagag
     1141 cgccgcggtg ccgacaagga acgacatggc ggcggcggtc agagggtggc cgagccggga
     1201 ggccgcctcg gcgttgatgg gagcctggag cgcgatgagc ccaccggcaa gaagcgcgat
     1261 caaggtgagc gaaaactctt ggagcgacat ggatggccct cttggcgtca agacgtgaga
     1321 tccgaggcga tgcgcgccga aaggatcttg tcgggcgaga ccggcggctc gccgcgattg
     1381 atcttgtcga cattctccat gccctcgatc acccggcccc acaccgtgta gtgacggttg
     1441 aggaaggagg cgtcgtcgaa gcagatgaag aactggctgt cggcggaatc gggcgcttgc
     1501 gcgcgcgcca tcgatacggc gcctcttgta tggggctcgg cgctgaactc ggcgccaagc
     1561 ttcttccccg agccgcccgt gcccgtgccg gtcgggtcgc cggtctgggc catgaagcct
     1621 tcgatcacgc ggtggaacac cacgccgtca tagaagcctt ggcgggcgag ttccttgatg
     1681 cgggcgacgt gatttggggc aaggtcaggg cgcatcgcca ccgtcacgcg gccctttgtc
     1741 gtctcgatga tgagcgtgtc ttcagggttt gccatgatcg ccatcctcct cgtaggctac
     1801 ttttcgatgg tcgccgtttt gatgaaatca agctcgggaa agtccttttc cagataggcg
     1861 ttgccctgct gctgaatgcg gccctggtcg ggtccgttcc cgtgcggggc gccttcgcca
     1921 taccccttgt agagcgaatc cacgacctcc atgcccgaca ccactttgcc gaagggcgag
     1981 aagccctgcc cgtcgagcgg agcattgtcg ccgaaattga tgaacacctg agtcgtgcgc
     2041 gtgttcggcc cggcggtggc gaaggtgatc gtgccgcgcc gattgctctg cttgaccgga
     2101 tcgtccttga tgttggcgtc gcgccagacg ctggcgatgc cggggtcgcc gttgatgccg
     2161 aactgcacca tgaagccgtc gatcacgcgg aagaagcgcg cgttgtcgta gaagccgttc
     2221 ttgaccagat tgtagaaccg atcggcgccg ttcggcgccc agtcgcgatg cacctcgacc
     2281 acgaaggcgc ccttgctggt atcgaatttc gccttgtaga ccgcaggcgc cttgtcgttg
     2341 agggtcgcgg gatcggagag attgacggcg cccgcgccgc tcgtccaggc gacaagagcg
     2401 agcgcgagcg cggctgcaaa cgcaaaactg acctgaagtc tcatgatgcc tccttggtaa
     2461 ggcgtgcagg gataggacta gcgggcaaat ttcttcttga gggccgcggc cacgaccggc
     2521 ggcacgaagg acgaaacgtc gccgcccatt gcggcgatct ggcggacgag gctcgccgcg
     2581 atatggcgcg cgtcgggcga agaagccaga aacacggtat ctatctccgg cgcaagcgcc
     2641 tggttcatgc cggccatctg catctcgtag tcgaaatctc cggcgttgcg caggcccctg
     2701 atgacgaggc tggcgccggc agccttcgcg gcgtcaaccg tcagattgcc gtaggtcgtc
     2761 acgtcgagct tcaagccggc cttcttggcc acgggatcga gcaccaccga ggtcagtttc
     2821 acgcgttcct cggcagtgaa gagaggatgc ttgtcgtggt gggtgccgat ggcgatgacg
     2881 agcctgtcga cgagatgggc cgcccggcgc acgatgtcga cgtgtccgaa ggtcacggga
     2941 tcgaagcttc cggggtaaag gccgacgcgc ataccggcgg ttccagacgc gacggtcgcg
     3001 gcccttgttg ggtcgcagga ttgcccggcc gcgcgggcca tctttagccg cgcaggtcgg
     3061 cgatgggcaa ggcgttattc ggctcgccaa tccccgaatg cggcgaaggg tccgacgcag
     3121 gtgcgagggg cctgacccgc cgcccggagt cgctacttga tcggcgcggc ctcggcgcca
     3181 tggccgaaga tggacccgag attgaagcgg tagccgaggc cggcggagaa gatccacgga
     3241 tcgacgtcgg catcgccgac gacattgacg ccgccgacgg cggcggcatt gcgccacgtg
     3301 accgtggtgt cgagccaggt cttcttgacg tcggcgttga gcgaccaacc gttgccgagc
     3361 gacacgtcaa ctcccgcctg caaggtaaag ccccaggcgt cgtcgatatc gactttggtc
     3421 gcgccgatga cattggcgcc agtgccggtg tcgaagaaat cgatgtatga acccccgcgc
     3481 cgacataggg cttgaagctg cccataatgg cgaagtggta ttgcagggtc agggccggcg
     3541 ggaaaatcca tgtgtcggcg atctcgccga aagcggcgat ggttcccttg ccgtcgattt
     3601 cgtgcttggc gaagcaacag aaaagctcga ccgcgagatt gttgttgaaa aagtaggtca
     3661 aggtaagggc cgggataact tcggtgctga catcggcgtc agctccgggg atcacggcgc
     3721 cgcccgcgct caccgtcgcc tcgctgtccg gatcgaccac ggtgccgaga gcgcgcacca
     3781 tgaagttccc attgtcgctt ccggccatgg cgggaaccgc cccgccgatc acggcaagcc
     3841 ccaacccgac catggccatc gcgcttcgcg ccggaccagc catcccttgt ccccatcctt
     3901 gcgaaagtcg tcccgcattc ttgggatgct gcgggcttcg atcaagcgcc gaccggaccc
     3961 ggtttctgac tgaaatcaga tctcggcgct tcctttccgc cctgttgctg ccgcgccaca
     4021 gattgtttga ctcaggtcag gccccccgct tgatcgagat caaggcaagc gatccggcca
     4081 cagccgacag cttgtgctcg ggcgattccg atctgccggc gggaaagacc atgacgacga
     4141 tcgcgagcgt caagacactg ggcggcgtgg ctcaagccga tggttcgtct catcccggtt
     4201 tcaaaggcac gccttcagcc gccctgcccg gagcgctcgg cagtcatgcc accgtcttgc
     4261 ggccacgacg cggacacgcg ctcgcgctga cgggggagaa cggcgaaatg gcgttcttcg
     4321 tccgcttcgg ggcgctcgtt ctgaatatcg ccttgcccaa ttcgacgcgg caggtcgcgg
     4381 cgcttctctt tccgggcgat ttgatccggt cgagcttcgc gccgcccgac gccgaggcga
     4441 cgctcgtcgc tccggtcgcg gccgaagtct tgcgcatgcg cttcagcaca gtcgagacgc
     4501 tcgcggcgga cgatcccgcg gtcatgcgct ttctcaacga cagcgtcgcg agccgcgtca
     4561 ggcgccaggc cctccatgcc gcaacgctcg gtcagctcga ttgcgagcag cgcgtggcga
     4621 cgctcctggt cgaactcgcg ctgcggaccg gcgcgcgctc aacccaaggc gtggcgttcg
     4681 acttgccgtt caaccgtagg gatgtcgccg cctatctcgg gctcaatccc gatacgcttt
     4741 cgcggatcat gtcgcgcttc cgcggcgccc cacaccgatc gaaatcgcgt cgtcgtgcgc
     4801 gatttcgccg cgcttgccgc gcgcaccccg gcggcgaaat cgctgctggc tatgagcgcg
     4861 aacccaagct aggccggttc gcccgcttcc tcctcgccgc cgttctcctc ggaaatgtgc
     4921 tcgaccgaga cgacgcgttc gccttcgccc gtcgagaaca cgatcacgcc ttgggtcgaa
     4981 cgccccatga cgctgatgtc ctgcaccggc acgcggatca gctggccgcc atcggtgaca
     5041 agcatgatct gatcggtctc ctcgaccggg aaggacgcga tcagcttgcc gttgcgctgg
     5101 ttcacgacca tggcgatgat gcctttgccg ccccggcccg agatccggta ctcgtaagcc
     5161 gaggtgcgct tgccatagcc gttctccgac acggtcagca cgaattgttc atgcgcgccg
     5221 agctcggcgt agcgctcggg actgagaacc accgtctcct gaccgtttgt ttccgcttcg
     5281 acgtcctcgg tatcggcgct gccgcggcgg atgctgttcg cttgccgcac ataggcggtg
     5341 cgttcggcgg gagacgcttc cacgtggccg aggatcgcca tggagatgac ctggtcgccg
     5401 cgctcgagct tgatgccgcg cacgcccggc gactcgcggc ccttgaacac gcgtacatcg
     5461 gcgacgggga agcggatggc ctgaccgtcg gcggaggtga gaagcacgtc gtcctcctcg
     5521 ccgcacacct cgacgccgat gatgcgatcg cccgaatcga gccgcatggc gatcttgccg
     5581 ttctggcgca cttcgatgaa gtccgagagc ttgttgcgac gcacgctgcc gtaattggtg
     5641 gcaaacatcg cgtctagctt ctcccaggag gcgacgtcct cgggcagcgg caggatggtg
     5701 gtgatggttt cgtccttgtc gagcggcagg aggttgacca gggccttgcc gcgcgcctgg
     5761 ggcgccgcga ccggcaggcg ccacactgtc atcttgtagg ccatgccgcg cgtggaaaag
     5821 aacagaaccg gcgtgtgggt gttggcgatg aagatgcggg tgacgaaatc ctcctcgcgc
     5881 gtcgccatgc cggcgcggcc cttgccgccg cggcgctggg cgcgataggc cgagagcggc
     5941 acgcgcttga tgtagccctt gtggctgacg gtgacgacca tgtcctcgcg ctggatcagg
     6001 tcctcgtctt cgacctcgcc ctccatctcg aggatctggg tgcggcgcgg cgtgttgaac
     6061 tcattcttga tcgccgtcag ctcgtccttg atgatcgaga cgatgcgggc acgcgagcgc
     6121 aagatgtcga gatagtcggt gatctgctcg ccaagcgtct tcaattcgcc gcccacttcg
     6181 tcgcggccga gagcagtcag ccgctggagg cgaagatcga ggatggcgcg ggcctgctcc
     6241 tccgagagac gataggtccc cttggcggag accttgtggc gaggatcggc aatcagctcg
     6301 acgagcccca ccacgtcctg cgccggccag tcgcgggcca tcaaccgttc gcgcgccgcg
     6361 gccgggtcgg gcgccttgcg gatcacggcg atgacctcgt cgatattggc gacggcgatg
     6421 gccaggccga cgaggacatg ggcgcgatcg cgggccttgc gcagcaggaa cttgacccgg
     6481 cggccgatca cctcctcgcg gaactcggtg aaggcgcgga tcatgtcctt gagcgtgagc
     6541 tgctcgggct tgccgccgat cagcgccacc atgttgcagc cgaaggagga ttgcagcggc
     6601 gagaagcggt agagctggtt cagcaccaca tcggccatgg cgtcgcgctt cagctctacg
     6661 acgacgcgca tgccctgccg gtcggactca tcgcgaatat cggcgatgcc ttcgatcctc
     6721 ttctcgcgca ccagctcggc aatcttctcg accatcgccg ccttgttcac ctgatacggg
     6781 atctcggtaa tgatgagcgc ctcgcgctcc ttgcggaccg tctcggtcgt cacgcggccg
     6841 cgcatgagaa tggagccgcg gcccttgtga taggcggcac ggatgccgga gcggccgaga
     6901 atgagaccgc cggtcgggaa gtcggggccg gggacgatct ccaccagctc gtcgataccg
     6961 atggcggggt tgtcgagata ggcgatgcag gcgtcggtca cctcgccgag attgtggggc
     7021 ggaatgttgg tggccatgcc gacggcaatg ccgcctgccc cgttgaccaa gaggttggga
     7081 aatttggccg gcaggaccac cggctccgtc tcgcggccgt cgtagttcgg ctggaagtcg
     7141 acggtgtcgt tctcgagatc gtcgagcaac gccatggcgg ggcgcgccag cctcacctcc
     7201 gtatagcgct cggccgcggg cggatcgccg tcgaccgagc cgaaattgcc ctgcccgtcg
     7261 acgatcggca ggcgcatcga gaagtcctgc gcgagccgga ccagagcgtc gtagatcgcc
     7321 tggttgccat ggggatgata cttgcccatg gtgtcgccgg tcacgcgcgc cgatttgttg
     7381 tagggccggt ccggcgtgta gttgttctcg ttcatcgaat agaggatgcg gcggtgcacg
     7441 ggcttgagcc cgtcgcgcac gtcgggaagc gcgcgcgaca cgatcacgct catggcgtaa
     7501 tcgagatagg agcgcttcat ctcgtcgatg agcgagatgg gtctgatgtc ggagggtgcg
     7561 ggaccctcga cctctggttt gtcgtcgtca gccaaggaaa gagaagcctg ttcgcaagag
     7621 gtgaccggcg gtcacgaagc aggcggcagg acggaaagtt cctccccacg cgacgcccga
     7681 acgatgaccg aaagacgact ttcctatagc tgatttgggc gcgttttcgc aaccccgcag
     7741 acgtccctcg cggcgtcttg attcgtcaat tgtttcagag ttttagggtg gggttgacgg
     7801 gccggtttac cctttgtcat caagcgctat tcgtcagcct actgagagtc acccgaaggc
     7861 gaattcgagg gaacgaaccg gttgctgaga cgttgttcct gcacctggtc agaggcacat
     7921 agcctcggcc cgatcgcacc gcgatcacca tccaacccca tgagaaagat acggagcgaa
     7981 acaatgaacc gatttctcac aaccacggct gtgggcctgt tcttgggtct cgcgcctgtc
     8041 attgcgcagg atctgcctcc ggccaacgat gcgcagaccc cgccggcggc cagtcagccg
     8101 tcgcaaagtc ctcaggtgag ccccgatccg gcacagccat ctgatccggc ggcgccgatc
     8161 ccgggcgact cctcccaggt ctcgccttct cccatggagc cgagtgacga tgccgccccg
     8221 ccggcgcaat cttcacaggc accggagtcg atcgagccgg ccgcaccggc cgcgccgaag
     8281 tcagcggaag cttccgacag cccgcaattc ctgtcgaagc aagccagcga cgattggctc
     8341 gccagcaatc tgatcggcca atcggtctat aacgcgcagg acgagatggt cggcgacatc
     8401 aacgacctcg tcaccgacca cagcggcaag atcgtcgccg tgttggttgg tagcggcggc
     8461 tttctcggcc tcggcgaaaa agacgtggcc ttgcgcttcg aggatctgag gctggcgcgt
     8521 gaagataacg acagcatcaa gatcgtcgcc gacgtgagca aggagacgct cgccgcggcg
     8581 cccgattatg agactctcgc ggagcaggag gtcaccgtgg gttcgaccaa ggacgaccgc
     8641 gaggatatcg gtggagagac gacgaactaa gctctctcgc ctgtaagcgg taaagcgggg
     8701 cccggccgtt cctcggccga gccctttttt tgtgcggcca atgtgaagac cgccatgcac
     8761 ttggtcatgc ccggacttga tccgggcatc catgccgttt cgtttcagcg agatgaggcg
     8821 ccaccggaat ggattgccgg gtcaagcccg gcaatgacgg gcacaaacca actcgccgcg
     8881 cagacgggct gagccgtcag aatggaattt cgtcgtcaag ctccttgtcg aaggagcttc
     8941 ccttgccgcc gccaccggca aacgggccgg atcgcccgaa atcgccgttg cgctggtcgc
     9001 tctcacccgt gccgtcgcgc ggcttgccgt cgagcatggt gagctcgccg cgatattgtt
     9061 gcagcaccac ttcggtcgag tagcgatcct ggccggattg atcctgccat ttccgcgtct
     9121 gaagctggcc ctcgatgtaa accttcgacc ccttcttcag atattgctcg gccacgcgcg
     9181 cgaggttctc gttgaagatg acgacccggt gccactcggt cttctcgcga cgttcgccgg
     9241 tcgccttgtc gcgccagttt tcgctcgtgg cgaggcgaag gttgacgatc ggtcgcccat
     9301 cctgggtttg gcggacttca ggatcggcgc cgagattgcc gacgaggata accttgttca
     9361 ccgaaccggc catgggatga gacctccgcg cggggcttcg ctcatgtgag ccccggtcaa
     9421 atctagcgcc ctcgaggcgc ggcctgccag acggcaaaga tcctgtccac agcccaaggc
     9481 ctacccgggc tttgtacacg ttttgttctc atctgtccag tcttgcttaa cgccttccca
     9541 ttttgaggct tgcggcagcg cggcctatgc cttacccgca aacgttcggt gagcgtaggc
     9601 ctgactcttg gctcgtcggg aaggaagtaa tgcagcgcac cgtcgaccaa cggcatgcca
     9661 ccaagcgccg ccccagcgag cggcgccatc cgcgttcgca attcctcggc ttcacgccgc
     9721 ggcagtggcc tttcatcttc gtcctgatcg gcaattggct gttcgctgcc gccttcttcg
     9781 tcctggccaa gttcctgtgg gactgggttc cgaccgagtg gaccgtcggc gaccgcatcg
     9841 cgctcgtgat caaggacgcc gtcgccgcca ttctgccggc ggtggtcgcc atctgcatcg
     9901 tcgccgccca gcgcctcgac ccgtcgatgt tcgtcggccg cacccctaag cccaactccg
     9961 cggtcgacat caataaccga ttcattctca acacggtcga gcaattcatc ctctacttca
    10021 tcggtaatgc gggactcgcc ctctactgc
//
LOCUS       DASUQP010000079         8844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1806482, whole genome shotgun sequence.
ACCESSION   DASUQP010000079 DASUQP010000000
VERSION     DASUQP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8844
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806482"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFY74_03600"
     CDS             <1..869
                     /locus_tag="VFY74_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930826.1"
                     /translation="KSTKFSISTLPTKDPISVLLEYVVEKNFKLPALVYAILSVLLVL
                     VLIRTAIRYFVRSGKPAPLNAYFVFYLTAWAILPLVFVYITFFALGAERYMGRYFAFC
                     FPPLPILLVLAIEQAVESLNVARPWLRFSLSRHYLRYALLYALAICAVFALPRTYAGA
                     ISVKEPYRDIARSIVKLVEGTTGASFAVYEASHTTHPMLDFYMKRMSTRVRLIGTIGV
                     DKDGRDPLAGVDAVMSGKDYLVIAFTHKQPKHFPLMMRQLSKEYDLVASQLLRSGRGY
                     MMWTKRAADQAR"
     gene            complement(1103..1603)
                     /gene="msrB"
                     /locus_tag="VFY74_03605"
     CDS             complement(1103..1603)
                     /gene="msrB"
                     /locus_tag="VFY74_03605"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5930827.1"
                     /translation="MLTRRDILLGGAALATLAVAGAGALRWRSGGAGAATGPFEVTHT
                     PDEWRKLLTPEQYAILREADTERPFTSPLNNEKRKGVFTCAGCDLPLFSSETKFDSGT
                     GWPSFHAPLEGAVKQDTDYMFGLVREEVHCRRCGGHLGHVFDDGPKPTGLRYCINGLA
                     LKFVPA"
     gene            complement(1772..3187)
                     /locus_tag="VFY74_03610"
     CDS             complement(1772..3187)
                     /locus_tag="VFY74_03610"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX5930828.1"
                     /translation="MTSLLSIGGDGTLGLERLQLLSDALLALSFFAVAGALIFIYRNR
                     EGHSPGVTVLVALFALFMVSIGLAHFVSLAAIVMPGQLGVEPEPVLTAIGAVLAAAGA
                     ALLWWLAPRWLRLPSRDRLQSEVAAHLRTLDELQSSRHRLEERVDARTKELAEAKQRI
                     EIALRGSPISVFSQDRDMRFTWAHNAPAGFPEELTGKTDAEILPQHAAEKIMAAKRKT
                     METGTAEELETNFELFGRKRSFYLMIEALRDEHGNVLGTTSVAVDISERKANEDQLRL
                     LLRELTHRCKNLLAVIHAIARQTAARTRSVDDFLDSFSARLVAMGCSHDLLIADDWHG
                     ASLRMLVERQLGEHADRFGDNIAIEGEDVMLKPEAVQNLGLALHELAANAEKYGSLSA
                     PHGQVSIRWQFCEEASKLQLVWQEKGGPPVTPPERSGFGRAMIETVVGRALEGDVMLS
                     FPPKGVRCEIVIPSAQVISRG"
     gene            complement(3630..3974)
                     /locus_tag="VFY74_03615"
     CDS             complement(3630..3974)
                     /locus_tag="VFY74_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5930829.1"
                     /translation="MAASRILVVEDEFLIALDIAGVLERAGLTVIGPAANVADALDAI
                     ARETVEGALLDAHLGGEPASRIADALVARGIPFAFVSGSGRDHLPAPHRNVPLVKKPF
                     TGNDLLAAVARF"
     gene            4136..4291
                     /locus_tag="VFY74_03620"
     CDS             4136..4291
                     /locus_tag="VFY74_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX5930830.1"
                     /translation="MLTTILIIILILLLLGAFPAWPHSRGWGYGPSGVVGLVLVIVLI
                     LVLLGRI"
     gene            complement(4313..5122)
                     /locus_tag="VFY74_03625"
     CDS             complement(4313..5122)
                     /locus_tag="VFY74_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5930831.1"
                     /translation="MTTSKAIAPHLPYLRRFARALAGTQAAGDAYVAATLEVLIEDPA
                     MFDNSLDPRVALYRTFVGLWNSVDVNLKPESLTDVPTAVDRKLESITPLPRQAFLLCA
                     VEGFTPAEAAQVLGVDVGKVDELIDLAGREIAAQVATDVLIIEDEPMIAMDLESIVEG
                     LGHRVTGVARTHAEALSAVDQRKPGLVLADIQLADGSSGLDAVNEMLVSFAVPVIFIT
                     AYPDRLLTGERPEPAFLITKPYQPDTVKAIVSQALFFERRAQPRDRPQMSA"
     gene            5405..5614
                     /locus_tag="VFY74_03630"
     CDS             5405..5614
                     /locus_tag="VFY74_03630"
                     /inference="COORDINATES: protein motif:HMM:NF037848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEX5930832.1"
                     /translation="MQRVSDEMDHGAPKIAAGPEAAKAGSGLTPALQAHIGRHVRAVF
                     DEVAREPVPEHLLRLLKDLERSGDK"
     gene            5615..6148
                     /locus_tag="VFY74_03635"
     CDS             5615..6148
                     /locus_tag="VFY74_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5930833.1"
                     /translation="MRRKVSSSLREQLVTEIGSLRAFAVSLCGDKERADDLVQETLFK
                     AWNHLESFKEGTNLKAWLFTILRNTYFSERRKRRREVEDADGAYAARLATHAEQYGHM
                     DMQDFRAALTLLPDDQREALILVGAAGFSYEEAAEISSCAVGTIKSRVNRARNRLATV
                     LGLEPQESGVEGLIKTA"
     gene            6262..6519
                     /locus_tag="VFY74_03640"
     CDS             6262..6519
                     /locus_tag="VFY74_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930834.1"
                     /translation="MTRRAIDLDQAKLDPGSVFCTPEEVLGAEELSPEEKRVILARWE
                     ADAEALLRATEEGMPPSDNRSPAELLRAVHEAMLRLEPRAA"
     gene            6612..7211
                     /locus_tag="VFY74_03645"
     CDS             6612..7211
                     /locus_tag="VFY74_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930835.1"
                     /translation="MLVAKPNLYAVQTSAVSWSPPIFLLDPCSNGVRREMENEHPSEF
                     GTQSTTSENKAEKEWSEFERDLDRLGRQLAELQMHTAALGTHFVSSLEARFEEVRNRA
                     ESFRKEREVELELMRQRAAREAESAFADMRQRSSEAARQAWERSEPLRQGAKDVGEGL
                     VRAWGEIWASFGKAAGRLHTETRANGPTPVTTQDRHERT"
     gene            complement(7338..>8844)
                     /gene="pabB"
                     /locus_tag="VFY74_03650"
     CDS             complement(7338..>8844)
                     /gene="pabB"
                     /locus_tag="VFY74_03650"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEX5930836.1"
                     /translation="ISDLTLSWTRDDYDRAFAKVRDYIAAGDVYQINLTLKYRFAFAS
                     DPVALYAALRRQQRVAYGALIHTGELDVLSLSPELFFRREGKHISTRPMKGTAQRGRT
                     PREDARLKTWLAVDEKQRAENLMIVDLLRNDLGRVAKIGSVEVTDLFTVETYRSVHQM
                     TSGITAELRSDMGLKDLLRALFPCGSVTGAPKVHAMEIIRELEAEPRGVYTGAIGHIA
                     PSGDAQFNVAIRTVVLAGAKGEMGIGSGIVADSKAESEFDECLLKAHFLTKFDAPFEL
                     IETLRFEHAQGFHLLERHLARLQSSAAHFGYPFSRDAVIAALDAEAARVETPVALVRL
                     LLAEDGAITVTSSAITLPTKDTVWRFVISDRRLDEKDAFFYHKTTRRDFYEREMERQK
                     ALNACDEVVFLNSKGELTEGTRTNLFVELDGRLFTPALACGLLPGTLREELLDLPRAA
                     ASEAILTPQDLLAADRIYLGNSVRGLVRAELIGPVQEAERERTPAHAAAGS"
BASE COUNT         1686 a         2751 c         2833 g         1574 t
ORIGIN      
        1 aaaagagtac caaattttcg atttcgacct tgccgacgaa agatccgatc tccgtcctct
       61 tggaatacgt cgttgagaag aatttcaaat tgccggcact cgtttacgcg atcttgtccg
      121 tcctcctggt gctcgtcctg atccggacgg cgatccgtta ctttgtacgc tccggcaagc
      181 cggcgccgct caacgcctat ttcgtgtttt atctgaccgc ttgggcgata ttgccattgg
      241 ttttcgtcta catcacgttt ttcgccttgg gcgccgagcg ctatatgggg cgctatttcg
      301 ctttctgctt tccgccgctt ccgatcctgc tcgtgctcgc catcgagcaa gcggtcgaaa
      361 gtctcaatgt tgcccggccc tggctaaggt tttcgctcag tcggcactat cttcgttatg
      421 cgttgctcta tgcgctggcc atctgtgccg tcttcgcgct gccgcgcacc tacgcgggtg
      481 cgatttccgt caaggaaccg tatcgcgaca tcgcgcgctc gatcgtcaag ctcgtcgaag
      541 gcacgaccgg cgcaagcttc gcagtctacg aggcttccca tactacccac cccatgttgg
      601 acttttacat gaagcggatg agcacacgcg tgcgtctcat cggtacgatt ggcgtcgata
      661 aggacgggcg cgatcccctc gccggggttg atgccgtgat gtccggcaaa gattacctcg
      721 tgattgcttt tacgcataag cagccaaagc attttcccct gatgatgcgg cagctcagca
      781 aagaatatga cttggtcgcg agccaattgc tccggagcgg gcggggttac atgatgtgga
      841 cgaagcgtgc ggccgatcaa gcccgctgac acgggcgtgt gggcatctgg cgatcggcgg
      901 cacaaaaaga gcgaaagggt cggacccgac cctttcaccc ccgtacacgg ctgagcggga
      961 acgcaacaca aaccccgccg aacccgccta acgcccaggc gtccgacagg ttccacgcga
     1021 cctcaaggaa aatgcgggac gtccggaaat ggctcggaat ccgcgcggga gggccaaaga
     1081 accgtagccc ggattggcgc ggctaggcgg ggacgaattt cagggcgagc ccgttgatgc
     1141 agtagcgcag gcccgtgggc ttgggaccgt cgtcgaacac atggccgaga tggccaccac
     1201 agcgccggca atgcacctcc tcgcgcacga ggccgaacat gtaatcggtg tcctgcttca
     1261 ccgcgccttc gagcggcgca tggaagctcg gccagccggt gccgctgtcg aacttcgtct
     1321 ccgaggagaa gagcggcagg tcgcagcccg cgcaagtaaa gacacccttt cgcttctcgt
     1381 tgttcaaggg actggtgaag gggcgctcgg tgtcggcctc gcgcagaatc gcgtactgct
     1441 cgggcgtgag cagcttgcgc cattcatccg gcgtgtgcgt cacttcgaac gggccggtcg
     1501 cggcaccggc accgccgctg cgccatctga gcgcgccggc acccgccacc gcaagggtcg
     1561 cgagagcagc accgcccaag agaatgtcac gtcgcgtaag catcgtcgct ctttaggtcc
     1621 tctctggtct taagcccacg cacgcgccga tcggctcgcg tctccgtcac gaccggtccg
     1681 cataaatgta ggcgctcagg gccgcgccgc aattcaactt gcgcgcacgg aagatcacgt
     1741 cccggtgtcg cgcgcccccg gaacactcgg cctagccgcg ggagatgacc tgcgccgaag
     1801 ggatgacgat ctcgcagcgc acgcctttgg gcgggaagga cagcatgacg tcgccctcaa
     1861 gggcccggcc gaccacggtc tcgatcatgg cccggccgaa accggagcgc tccggcggcg
     1921 tcaccggtgg cccgcccttc tcctgccaga caagctgcag cttggaggct tcttcgcaga
     1981 attgccaccg gatgctgacc tggccgtgcg gcgcggacag agaaccatat ttttcggcgt
     2041 tggcggcgag ctcatgcagc gcaagtccga gattctgaac cgcctccggc ttcagcatca
     2101 cgtcctcgcc ttcgatggcg atgttgtcgc cgaagcggtc ggcatgctcg cccaattgcc
     2161 gttcgaccag catgcgcagg gacgcgccgt gccaatcgtc ggcgatgaga agatcgtgcg
     2221 agcagcccat agccacgagc cgggcgctga agctgtcgag gaagtcgtcg acggagcggg
     2281 tacgcgcggc ggtctgcctc gcgatggcat ggatgacggc gagcagattc ttgcagcgat
     2341 gggtaagctc tcgcagcaga aggcggagct ggtcctcgtt ggccttgcgc tcgctgatgt
     2401 cgacggcgac cgaagtggtg ccgagcacgt taccgtgctc gtccctcaac gcctcaatca
     2461 tgagatagaa agagcgcttg cggccgaaca gctcgaaatt ggtctcaagc tcctcggccg
     2521 taccagtttc catcgtcttg cgcttggccg ccatgatctt ctcggcggcg tgttgcggga
     2581 gaatttcggc atcggtcttg ccggtcagct cttcggggaa gccggccggg gcgttatgcg
     2641 cccaggtaaa gcgcatatcc ctgtcctggc tgaacacgga gatgggggag ccccgaagcg
     2701 cgatctcgat gcgctgtttg gcttcggcca actccttcgt gcgcgcgtcg acgcgctcct
     2761 cgagtcgatg ccgcgacgac tggagctcgt cgagggtccg caaatgagcg gcgacctcgg
     2821 attgcagccg gtcgcgcgag ggcagccgca accagcgcgg tgcaagccac cagaggagag
     2881 ctgcgcctgc ggcggcgagc acggccccaa tagccgtcag gaccggttcc ggttccacgc
     2941 cgagctgccc tggcatcaca atcgccgcca gagagacgaa atgggccaga ccaatcgaaa
     3001 ccatgaaaag ggcgaacagc gcgaccagga ccgtcacgcc gggactatgg ccctctcgat
     3061 ttcgataaat gaagatcaga gcgccagcga cggcgaagaa ggacagggca agcagggcgt
     3121 ccgacaggag ctgaagccgt tcgagcccca aagtcccgtc tccaccgatc gacagcaaag
     3181 aggtcatatc gccagccaat ccgccgcaag aatccgtcgc cgggagggaa tttggacccg
     3241 ccagcggcgc gacgcttcac ggacctcaaa aaactgggct gcttgggccg gttccgcaag
     3301 gcgcaaggga gcaactgccg ggaactcttg cgcgctactt gcgttgagtt gccgttcgag
     3361 ggcacgcgaa ccatcgtccc gcccctcccc tggtgcgctc gaactcatcg cccgcaaccg
     3421 ccctcccccg gttgcgggcg tttcctttat ggggagtttt ttagagaaaa gcggcaatca
     3481 gagtcaccag gaccatgaga cgattgggtt cgcgcccgaa aacggccttc agcctgaggc
     3541 tgaaggcgga gcaggctgaa ccgccatgcc taagtctaca acccacgttc tatcaaatgg
     3601 ttgcaggctt gcgttgcggc cgagcggtgt cagaagcgcg ccaccgcggc aagcaagtca
     3661 ttgcccgtga atggcttttt cacgagcgga acattgcggt ggggggccgg caaatgatcg
     3721 cgcccggagc cgctgacgaa ggcgaaaggg atgccgcgcg caacgagcgc atcggcgatg
     3781 cggcttgccg gttcgccccc gagatgggca tcgagcagcg cgccctccac ggtttcacgg
     3841 gcgatggcgt cgagcgcatc ggcgacattc gcggccgggc cgatcacggt gaggcccgcc
     3901 cgctcaagca caccggcaat atcgagtgcg atgaggaact cgtcttcaac cacgaggata
     3961 cgcgatgctg ccattccgct tggcgccttt ccgtggaggc aaagtccggt gaccgctagt
     4021 ggccacgcct ggaaaagagt agactcatca tgggctcggc gccaatccaa gatgtttcaa
     4081 gggcattcgc ggggaacccg ccagccctgg gcgcccggca aaaaaggggg gtacgatgct
     4141 cacaacgatt ctgattatca tcctgatctt gttgctgctc ggagcctttc cggcgtggcc
     4201 gcatagccgg ggctggggtt acggcccgtc tggcgtcgtg ggcctcgtgc tggtgatcgt
     4261 cctcatcctg gtcttgctcg gcaggatctg acgcgcaaga ccgcgctcgc gcctaggcac
     4321 tcatttgggg gcggtcgcgc ggttgtgccc ggcgctcgaa gaaaagcgct tgcgatacga
     4381 tcgccttcac ggtgtcgggc tgatacggct tggtgatgag gaaggccggc tcgggccgtt
     4441 cgccggtcaa aaggcggtca ggataggcgg tgatgaaaat caccggcacg gcgaaggaaa
     4501 cgagcatttc attcaccgca tcgaggcccg aactgccgtc ggcaagctga atgtcggcga
     4561 gcacgaggcc cggcttgcgc tgatcgacgg cgctaagcgc ttcggcatgg gtgcgggcga
     4621 cgccggtgac gcgatggccg aggccctcga caatgctttc gaggtccatg gcgatcatcg
     4681 gttcgtcttc gatgatgaga acgtcggtcg cgacttgcgc ggcgatttcg cgacccgcca
     4741 ggtcgatcag ctcgtcgacc ttgccgacat cgacgccgag gacttgcgcc gcttcagccg
     4801 gcgtgaagcc ttccaccgcg caaagtagaa aagcctgccg cggcagaggt gtgatcgact
     4861 ccagcttgcg atcgaccgcg gtcgggacgt ccgtcaggct ctccggcttg aggttgacgt
     4921 cgacggagtt ccataggccg acaaaggtgc ggtaaagcgc aacgcgcgga tcgagcgaat
     4981 tgtcgaacat cgcaggatcc tcgatcaaca cttcgagcgt tgccgccaca taggcgtcgc
     5041 cggcggcctg ggtgccggca agggcccggg cgaaacgccg cagataaggg agatgaggcg
     5101 cgatcgcttt cgacgtcgtc atccagctat gaccttgtga taagccctga acagttccca
     5161 tttctcacaa accgggcttg gcgtggcaac ttaaaaccgc atcaaagcgc gttcaccgca
     5221 ccgggcaaag cccgcgttaa acgcatcggg cggaaattcg gttccagaag atggaaccaa
     5281 ttgaaggcct tgggcgtttt tgtgccctta agctcagaga atccggtatt ttgggttcgc
     5341 cgacacggac gtattggaaa cggttgtgaa ccgcaacgcg aagatcaagg atcgccggcg
     5401 aaaaatgcag cgcgttagcg acgagatgga ccatggcgcg ccaaaaatcg cggccggacc
     5461 cgaagcggca aaggccggca gcggcctgac ccctgccctg caggctcaca tcggccgtca
     5521 cgtccgcgcg gtcttcgacg aggtcgcacg cgaacccgta ccggagcatc ttctgcgctt
     5581 gcttaaggat cttgagcgaa gcggagacaa gtagttgcgg cgcaaagtgt cttccagcct
     5641 tcgagaacag ctggttaccg agatcggcag cctgcgcgcg ttcgccgttt cgttgtgcgg
     5701 cgacaaggaa cgcgccgacg atctggtgca ggaaacgctg ttcaaggcct ggaatcatct
     5761 ggagtcgttc aaggaaggca cgaacctcaa ggcttggctg ttcaccatcc tgcgcaacac
     5821 gtatttctcc gagcggcgca agcggaggcg cgaggtcgag gacgccgacg gagcctatgc
     5881 cgcgcgcctt gcgacgcacg ccgaacagta cggtcatatg gacatgcagg actttcgcgc
     5941 ggcgcttaca ctgcttcccg acgaccagcg cgaagccctg atcctcgtgg gcgcggccgg
     6001 cttttcctac gaggaggcgg cggagatctc gagctgcgcc gtcggcacga tcaagagccg
     6061 ggtcaatcgc gcgcgaaatc ggctcgccac cgtactcggt cttgagcctc aagagagcgg
     6121 tgtggaaggg ctcatcaaga cggcctgaag aagacggcct gaagcggtag ttcttttgct
     6181 gcgtgtcgtg cccatgtgcg gctatgctat ccagcaacaa aacaccccgt tgcggcgttt
     6241 gacggatgga gcagagcgcc catgacacgc agggccatcg atcttgatca agcgaagctc
     6301 gaccccgggt cggtcttctg cacgcccgag gaggtcctcg gcgcggaaga gttgtcgccc
     6361 gaagaaaagc gagtgatcct tgcacgctgg gaagccgacg ccgaggcttt gctcagggcg
     6421 acggaggagg gcatgccgcc ttcggacaat cgcagcccgg cggaacttct gcgcgcggtg
     6481 cacgaggcga tgctccggct tgaaccccgc gccgcatagc cagccccgcg cgccttccgc
     6541 ggcgctgatc gggcaacccc tctggccggg ctctccccca atcctccaag gttaagcacg
     6601 ccccttgatc gttgctggtg gcgaaaccaa atctatatgc tgtccaaact tctgctgtaa
     6661 gctggtcacc ccctattttc cttttggacc cctgttccaa cggagtgagg cgagagatgg
     6721 aaaacgagca cccttcggaa ttcggcacgc aatcgacgac gagcgaaaac aaagcggaaa
     6781 aggagtggtc ggagttcgag cgcgatctcg atcgcctggg ccggcagctc gccgagctac
     6841 agatgcacac ggccgcgctc ggaacccatt tcgtgtccag tctcgaggcc cgctttgaag
     6901 aggttcgaaa tcgtgccgag agtttccgga aggaacgcga agtcgagctt gagctgatgc
     6961 gccaacgggc cgcgcgcgag gccgaaagcg cgttcgccga tatgcgtcag cgctcgagcg
     7021 aggccgcacg ccaagcatgg gagcgttcag agccgctgcg ccaaggcgcc aaggatgtcg
     7081 gcgagggctt ggtgcgggcc tggggcgaga tctgggcgtc gttcggcaag gccgccggcc
     7141 ggcttcatac ggagacgcgc gccaacggcc caaccccggt cacgacccaa gaccgccacg
     7201 agcgcacata acctcgtcgc gcatgcctgg cgatcgtgcc gggcctcgaa agttcacgca
     7261 acgaaccgcc atgaccggcc gctccggtcg tggcggttct tgttttcgtc attccgggtc
     7321 ttcccgacaa acaaagctta tgagccggcg gccgcatgcg cgggcgtgcg ctccctctcc
     7381 gcctcttgca ccggcccgat cagctcggcg cgaacgagcc cgcgcaccga gttgccgaga
     7441 tagatgcggt cggcggcaag caaatcttgc ggggtgagaa ttgcctcgct cgcggcggcc
     7501 cgcggcagat cgagaagctc ctcgcgcagc gtgccgggaa gaaggccgca agcgagcgcc
     7561 ggcgtgaaca gccgcccgtc gagctcgaca aagagattgg tgcgcgtgcc ctcggtcagc
     7621 tcgcccttgc tgttgaggaa gacaacctcg tcgcaggcat tgagcgcctt ttgccgctcc
     7681 atctcgcgct cgtagaaatc ccgtcgcgtg gtcttgtgat agaagaaagc gtctttctcg
     7741 tcgaggcgcc ggtcggagat tacgaaacgc cacaccgtat ccttggtcgg gagcgtgatg
     7801 gcgcttgagg tcacggtgat ggcgccgtcc tcggcaagca gcagccgcac cagagcgacc
     7861 ggcgtttcca cgcgggccgc ttcggcgtcg agcgcggcaa tcacggcgtc gcgcgagaac
     7921 ggatagccga aatgcgcggc cgaggattgc agccgggcga gatgccgctc cagcaggtga
     7981 aagccctgcg catgctcgaa ccgcagcgtc tcgatcagct cgaacggcgc gtcgaacttg
     8041 gtgagaaagt gcgccttcaa caggcactcg tcgaattcgg actcggcctt ggaatcggca
     8101 acgatgccgc tgccgatgcc catttcgccc ttcgcgcccg cgagtaccac ggtgcggatc
     8161 gcgacgttga actgggcgtc gcccgacggc gcgatgtggc cgatggcgcc ggtatagacc
     8221 ccgcgcggct ccgcctccag ttcgcgaatg atctccatgg cgtggacctt gggcgcgccg
     8281 gtgaccgagc cgcaaggaaa gagcgcgcgg agcaggtcct tcagccccat gtcggatctg
     8341 agctccgcgg tgatgccgga ggtcatctgg tgcaccgagc gataggtctc caccgtgaac
     8401 aggtcggtca cctcgaccga cccgatcttc gccacgcggc cgagatcgtt gcgcaggaga
     8461 tcgacgatca tgagattttc ggcgcgctgc ttttcgtcga cggcgagcca ggtcttgagt
     8521 cgggcatcct cgcgcggcgt gcgtcctctc tgcgccgtgc ctttcatcgg acgcgtcgag
     8581 atgtgcttgc cctcgcggcg aaagaacagc tcgggcgaaa gcgaaagaac gtcgagctcg
     8641 cccgtgtgga tgagcgcacc gtaggccacg cgctgctggc gccggagcgc cgcatagagc
     8701 gccacagggt cactggcgaa cgcgaagcga tatttcagcg tgagattgat ctgatagacg
     8761 tcgccagcgg cgatgtaatc ccgcaccttg gcgaaggcac ggtcgtagtc gtcccgagtc
     8821 caggagagcg tgaggtctga gatc
//
LOCUS       DASUQP010000080         5837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_35240,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000080 DASUQP010000000
VERSION     DASUQP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35240"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFY74_03655"
     CDS             complement(<1..1163)
                     /locus_tag="VFY74_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5930837.1"
                     /translation="MTAALEPSLDSFKCRRTLKADGVAYDYFSLKEAEANGLQGISSL
                     PFSLKVLLENLLRHEDGRTVTADDIRAVALWLRERKSDREIAFRPARVLMQDFTGVPA
                     VVDLAAMRDAMAALGGDPRKINPLAPVDLVIDHSVMVDAFGSGQAFQINVDKEYERNR
                     ERYAFLRWGAGAFDNFRVVPPGTGICHQVNLEYLAHTVWTKENGAGTIAFPDTLVGTD
                     SHTTMVNGLSVLGWGVGGIEAEAAMLGQPISMLIPEVVGFRLTGALKDGVTATDLVLT
                     VTEMLRRAGVVGKFVEFFGSGLGHLPLEDRATIANMAPEYGATCGFFPIDEETLTYLE
                     ATARKRNRIALVEAYARAQGLYRDGDTPDPAFTNTLHLDLSDVEPSIAGPKRP"
     gene            1410..2021
                     /gene="ccmA"
                     /locus_tag="VFY74_03660"
     CDS             1410..2021
                     /gene="ccmA"
                     /locus_tag="VFY74_03660"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX5930838.1"
                     /translation="MGLAALDISCQRGGRTIFRDVSFRLAPGEALLVTGPNGAGKTSL
                     LRQIAGLLPLAAGALRLEGGEPDAAIPELCHYVGHANAVKASLSVGENLAFWADFLGE
                     RRPDLEAALAAFGLAPLAEFPAGLLSAGQKRRLALSRLLAAIRPIWLLDEPQTSLDTA
                     SIELLDTALKDHLQAGGIAVVASHAALPTKHKKRLALGKERVS"
     gene            2018..2686
                     /gene="ccmB"
                     /locus_tag="VFY74_03665"
     CDS             2018..2686
                     /gene="ccmB"
                     /locus_tag="VFY74_03665"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5930839.1"
                     /translation="MSAGLALLARDVKLAFREGGAIGTALGFYLLVIAITPLGLGPDL
                     NLLARIAPGMLWVALLLAALLSADRIFHNDYEDGALDVLTTGPLPLPLVAAIKSFAHW
                     LTTGVPLALLAPLLGLLLNLPAQASPLLVLAMLAGTPAVSFIAAIGASLTLGLKRSGL
                     LLALLVLPLYVPVLIFGVATASAAITGPASPWPPFLMLCGISLASVVLAPLAAAAALR
                     TALR"
     gene            3141..3878
                     /locus_tag="VFY74_03670"
     CDS             3141..3878
                     /locus_tag="VFY74_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEX5930840.1"
                     /translation="MPSLANPTRFLDLAGRLIPWLAVLTAILFGIGLYLAFFVAPPDY
                     QQGETVRIMFVHVPAAWLAMSGYALIAVASLGTLIWRHPLADVAAKAAAPIGATFALI
                     ALVTGSLWGKPMWGTYWVWDARLTSMLVLFLLYLGLIALWQAIEEPGRAGRAAAILGL
                     VGAINIPIIKFSVDWWNTLHQPASIVRVGAPTIDPSMLGALAVMALAFTLLFVLLHLI
                     AMRAEILRRRVRAMQRTQVEHAALAET"
     gene            3890..4093
                     /gene="ccmD"
                     /locus_tag="VFY74_03675"
     CDS             3890..4093
                     /gene="ccmD"
                     /locus_tag="VFY74_03675"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX5930841.1"
                     /translation="MPDLGPHAIFIFAAYGVAFAAVAALAFAIVADDRKQRRLLADLE
                     SRGIRRRSAVTGRAGEPARGPRP"
     gene            4090..4689
                     /locus_tag="VFY74_03680"
     CDS             4090..4689
                     /locus_tag="VFY74_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX5930842.1"
                     /translation="MTEERRAAPAPFRRFGPALPLAIFAVLAGLFWYALQSGDPSRLP
                     SPLIGKKVPDFTLPPLEGLTAGGEQVPGFAASELAEGDPTLVNVFASWCLECQTEHPL
                     LLALGQSPGIRLYGIDYKDDPEAARRFIGRYGNPYARVGTDRSGRVAIDLGVYGVPET
                     YVVRGDGTIAYRHVGPLTEQVIKVKILPLLRSPTDKTQG"
     gene            complement(4686..5132)
                     /locus_tag="VFY74_03685"
     CDS             complement(4686..5132)
                     /locus_tag="VFY74_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5930843.1"
                     /translation="MAKPGKDLDRSVTHLLHRAGQCAAEIFASEARTPGLTPRQLAVM
                     TVIGDEEGLNQTELVERTGIDRSTMADLVARLSRRGLVQRRRTKEDARAYAIKLSPQG
                     ARLLRESQPAAAAADQRLLACLPAGKRQDFLDTLALIVSAAHKARR"
     gene            complement(5375..>5837)
                     /locus_tag="VFY74_03690"
     CDS             complement(5375..>5837)
                     /locus_tag="VFY74_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX5930844.1"
                     /translation="AFMAATLVSLAVARIRHQKVPVMLLVTGVIVLVFGGLTLFLRDE
                     TFIKLKPTIVYSMFALLLAAGLMLRKPLLELLFGSAFALTEEGWRKLTFRWIVFFVAM
                     AVVNELVWRNFSTDTWVSFKVFGFLPLTLLFALAQVPLLQRHGLAPQATKE"
BASE COUNT          943 a         1985 c         1846 g         1063 t
ORIGIN      
        1 tggggacgct tcggtcccgc gatcgacggc tcgacatcgc taagatcgag atgaagcgtg
       61 ttggtgaaag ccgggtcggg cgtgtcgccg tcgcgataga ggccttgcgc cctcgcatag
      121 gcctcgacca gcgcgatgcg attccgcttc cgcgcggtcg cctctagata ggtgagcgtc
      181 tcctcatcga tcgggaagaa gccgcaagta gcgccgtact caggcgccat attggcgatg
      241 gtggcgcggt cttcgagggg cagatgcccg aggccagagc cgaagaactc cacgaacttg
      301 ccgaccacgc cggcgcgccg cagcatctcg gtgacggtga ggacgaggtc ggtcgcggtg
      361 acgccgtcct tgagcgcgcc tgtcaggcga aagcccacca cctcggggat cagcatggaa
      421 atcggctggc cgagcatggc ggcctccgct tcgatgccgc cgacgcccca gccgagcacc
      481 gagagcccgt tgaccatggt ggtatggctg tcggtgccga ccagcgtgtc ggggaaggcg
      541 atcgtgcccg ccccgttctc cttggtccac accgtatgcg ccagatattc gagattgacc
      601 tggtggcaaa tgccggtgcc tggcggcacc acgcggaaat tgtcgaaagc gcccgcgccc
      661 cagcgcagga acgcgtaacg ctcccggttc cgctcgtatt ccttgtcgac attgatctgg
      721 aaggcctggc cagagccgaa ggcgtcgacc atgaccgaat ggtcgatgac gaggtcgacc
      781 ggcgcgagcg gattgatctt gcgcggatcg ccgccaagcg cggccatggc gtcgcgcatg
      841 gcggcaagat cgacgacggc gggaacgccg gtgaagtcct gcatgaggac gcgcgcggga
      901 cggaacgcga tctcgcgatc gctcttgcgc tccctgagcc acagcgcgac ggcgcgaatg
      961 tcgtcggcgg tgaccgtgcg cccatcctcg tgacgaagca ggttctcgag cagcaccttg
     1021 agcgagaagg gaaggctgct gatgccttga agcccgttgg cctcggcctc cttcaggctg
     1081 aaatagtcgt acgcgacccc gtcggccttc agcgtgcggc ggcatttgaa gctgtcgagc
     1141 gagggttcaa gggccgcggt caacgagcgc tccttcggcg acgatggcat ggagcaagcg
     1201 gaggcgcggc cagggctctg acgggacagc cccgctatcc cgcctcgaac ggctcccctt
     1261 ggttaaggct tgcgtgctca gggtctatat aaaagcgctt gcggcaacga gacaatgaca
     1321 cgaagagagc tttcgggcac gaattcctcc cggaacgctg ccttgccctg tcttcgactt
     1381 cgcgccaatt ccgagcaatg ccgcacgcga tcggcctcgc cgccctcgat attagctgcc
     1441 agcggggcgg caggaccatc ttccgggatg taagttttcg gcttgcgccg ggcgaggcgc
     1501 tcctcgtcac agggccgaac ggcgccggca agacgagcct gctccggcag atcgcggggc
     1561 tccttccgct cgccgcaggc gctctccgcc tggagggcgg agaacccgac gccgcaattc
     1621 ccgagctttg ccactatgtc ggccacgcga acgccgtgaa ggctagtctc agcgttgggg
     1681 aaaatctcgc cttctgggcc gatttcctcg gcgagcgtcg ccccgatctc gaagccgctc
     1741 ttgccgcgtt cggacttgcg cctttggccg agtttcccgc cggtctgctg tcggcggggc
     1801 agaagcggcg gcttgcgctg tcgcgcctcc ttgccgcgat ccgtccgatc tggttgctcg
     1861 atgagccgca aacctcgctc gacacggcat cgatcgagct gctcgacacc gcgctcaagg
     1921 atcatcttca ggcgggcggg atcgccgtcg tggcgagcca tgcggcgctg ccgacgaagc
     1981 ataagaagcg gctcgccctc ggaaaggagc gcgtttcgtg agcgcgggcc tcgctctcct
     2041 cgcacgcgac gtgaagctcg cctttcgcga aggcggcgcc atcggcacgg cgctcggttt
     2101 ctatcttctg gtgatcgcca tcacgccgct cgggcttggg cccgatctca atctgctcgc
     2161 ccgcatcgcg cccggcatgt tgtgggtggc gcttctcctt gcagcgctcc tctcagccga
     2221 ccgcatcttc cacaacgact acgaggacgg cgcgctcgac gtgcttacga ccggacccct
     2281 gcctctgccg ctcgtcgccg cgatcaagag ctttgcccac tggctcacga ccggcgtgcc
     2341 gctcgcgctg ctggcgccgc tcctcggact cctgctcaat cttcccgcgc aagcgagccc
     2401 gctcctcgtg ctcgccatgc tggccggcac gccggcggtg agcttcatcg ccgcgatagg
     2461 cgcaagcctc acgctcggac tgaagcgaag cgggctactt ctcgcgctgc tcgtgctgcc
     2521 gctctacgtg ccggtcctga tcttcggcgt ggcgacggcg tccgccgcaa tcaccggccc
     2581 cgcctccccc tggccgccct ttctcatgct gtgcggcata agcttggcga gcgtcgtgct
     2641 ggcgccgctt gccgcggccg cagccctccg caccgcgcta cgctgacccg ctcccgtttc
     2701 caaccaccgt gatggccggg cttgtcccgg ccatccacga ttttgacaac cccattcgta
     2761 gatcgattac aagaccccca ccccgacgcc gcgcaccgcg cgcgccggac ctccccacga
     2821 gggggaggtt taaaatagac acacgaccaa aactgcagat ccttcaccgc tccacataaa
     2881 atgcccgctc tccgccctgc cttcgccgaa gcgaagccgc ttcggcttcg cgcaggcagg
     2941 ttggggagag cacgcgcgcc attggcgcgc ggtgaggggt caattccgtc acaccgcgga
     3001 agcagactcg caggaatgcg tgggttgaag cgacccccca ccccgacgcc gcgaaacgcg
     3061 gcgccggacc tcccctcaag ggggaggtat gagaaggtgg cggattgcgc cctctaccta
     3121 aagccactag atcaggccag atgccgagcc tcgccaatcc gacgcgcttc ttggaccttg
     3181 ccggccgcct cattccgtgg ctcgcggttc tgaccgcgat cctgttcggc atcggccttt
     3241 atcttgcttt cttcgttgcg ccgcccgact accagcaggg cgagaccgtg cggatcatgt
     3301 tcgtccatgt tcccgccgcc tggcttgcca tgtccggcta tgcgctgatt gcggtcgcga
     3361 gcctcggcac gctgatctgg cgccaccctc tggccgatgt cgccgccaag gccgccgccc
     3421 cgatcggcgc taccttcgcc ctgatcgcgc tcgtcaccgg ctcgctctgg ggcaagccga
     3481 tgtggggcac ctattgggtg tgggacgcgc gtctgacatc gatgctcgtg ctgttcctgc
     3541 tctatctcgg actgatcgcg ctgtggcagg cgatcgagga gccgggccgc gcgggtcgtg
     3601 ccgccgcgat cctcgggctc gtcggcgcca tcaacattcc catcatcaag ttctcggtgg
     3661 actggtggaa cacgctgcac cagccggcga gcatcgtccg cgtcggcgcc cccaccatcg
     3721 acccctcgat gctcggcgct cttgccgtca tggcacttgc cttcactttg ctgttcgtgc
     3781 ttctgcatct catcgcgatg cgcgcggaaa tcttgcgccg gcgcgtccgg gcgatgcagc
     3841 ggacgcaagt cgagcatgcg gcgttggccg aaacgtaaat cggaaagcca tgcccgatct
     3901 cggtccgcac gcaatattca tcttcgccgc ctatggcgtg gcgttcgccg cggttgcggc
     3961 gcttgcgttc gccatcgtcg cggacgaccg caagcagcgc cggcttcttg ccgatctcga
     4021 atcgagaggg atcagacggc gttcggcagt tacggggcgc gccggagagc ctgcgcgagg
     4081 ccccaggcca tgaccgagga aaggcgcgcc gcgccagcgc ccttccggcg cttcggcccc
     4141 gctctaccgc ttgcgatctt cgccgtgctt gccggtctct tctggtacgc gctgcaaagc
     4201 ggcgatccct cgcgtttgcc ctcgccgctg atcggcaaga aggttccgga tttcacgctt
     4261 ccccctctcg aaggcctgac ggccgggggc gagcaggtgc cgggcttcgc cgcttccgag
     4321 ctcgccgaag gcgatcctac tctcgtcaat gtcttcgcgt cctggtgtct cgaatgccag
     4381 accgagcatc ctcttctcct cgctctcggc caaagtcccg gcatcaggct ctacggcatc
     4441 gattacaagg acgaccctga agcggcgcgg cgcttcatcg gccgctatgg caatccttac
     4501 gcgcgcgtcg gcaccgaccg ctcgggccgc gtcgccatcg atcttggcgt ctacggcgtg
     4561 cctgaaactt atgtggtgag gggcgacggc accattgcct accgccatgt cggcccgctc
     4621 accgaacaag tcatcaaggt aaagatcctg cccctgctcc ggagcccgac tgacaagacg
     4681 cagggctagc gccgcgcctt gtgagccgcg ctgacgatca gagcgagcgt gtcgaggaag
     4741 tcttgccgct tgccggcagg gaggcaagcg agcagtcgct ggtcggcggc ggcggcggcg
     4801 ggctgggact cgcggagcaa ccttgcccct tgggggctga gcttgatcgc gtaggctctg
     4861 gcatcctcct tggtgcggcg acgctggacg agacctctcc ggctgagccg cgcgacaagg
     4921 tcggccatcg tggagcgatc gatgcccgtg cgttcgacca gttcggtctg gttcagccct
     4981 tcctcatcgc cgatcaccgt catcacggcg agctggcgcg gcgtcagtcc gggggtgcgc
     5041 gcttccgaag cgaagatctc cgccgcgcac tgccccgcgc ggtgaagcag atgcgtcacc
     5101 gagcggtcga gatccttccc tggtttggcc atctcagcgc ctgcgtccgt atgacacgcc
     5161 cttcttcccg aaaaacgtgc accttccaaa tgttaacctt tgtgtcctga gaagtttatt
     5221 taatttacag ccaaacgggc gaacagcaaa tgtctgggtt gtcgcaagca cccgcgcggt
     5281 cgcggtagcc cacaatcggg aggctcgttt cgcctttgcc gggacgcggt cttggccgct
     5341 tctccaaagg gccgaatccg gctcttttcc ccgtttattc ctttgtcgct tgcggcgcca
     5401 ggccatggcg ctgcagcagc ggcacctgcg caagcgcgaa tagaagggtc agcggcagga
     5461 agccgaaaac cttgaagctg acccaggtgt cggtcgagaa attgcgccag acgagttcgt
     5521 taaccacagc catcgcgacg aagaagacga tccaccggaa agtgagcttg cgccaccctt
     5581 cctcggtcag cgcaaaggcc gagccgaaca gcagctcgag cagcggcttc ctcagcatga
     5641 ggccggccgc gagcagaaga gcgaacatcg agtagacgat cgtcggcttg agcttgatga
     5701 atgtctcgtc gcgcaggaag agcgtgagcc ctccgaacac gagcacgatc acgccggtga
     5761 cgagcagcat gaccgggact ttttggtgcc ggatgcgggc gacggcgagc gagacgagcg
     5821 tcgccgccat gaacgcc
//
LOCUS       DASUQP010000081        17330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1534870, whole genome shotgun sequence.
ACCESSION   DASUQP010000081 DASUQP010000000
VERSION     DASUQP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 17330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17330
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534870"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFY74_03695"
     CDS             <1..713
                     /locus_tag="VFY74_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930845.1"
                     /translation="VAVSFEAKLVVYGDPDDAPLPRKFLIKPENRLIVRDANGKTAVD
                     EPLCGNCTDIFDDKVSIVLPLAVAPLLRENKTIDMAVGLAGKEECRFKLDGESLRAGL
                     DWASERRTALAEARAEEKCTPPEGCFITSACCEAIGLDDNCFELRALRRYRDEVLAKR
                     PGGVNEIAAYYALAPSILGRVPRKGRKRLLRFLYVRYILPSALAATFGFDALAYRLYA
                     RMLRELTLAFAAEEMPSS"
     gene            1086..2162
                     /locus_tag="VFY74_03700"
     CDS             1086..2162
                     /locus_tag="VFY74_03700"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5930846.1"
                     /translation="MLRTVWVVGLVLAWLVGSGPVQGADNKSNADQAWLGLIAGSAAG
                     SETVLAADMASLFPQGDLGILPILGDAGAGNLARLEDLKVDIAFVSTDVLAEAAAKDD
                     SLRNRLELVTRLAPQEVHVLARADIGKLSDLSGREVNFGPPGSASAVTAAALFKALPV
                     EVEALSLDTTTAIERLKEGAIAAAVVVGGKPSPVVGAIPPNSGIHLLPVSFGATLATE
                     YLPTRLGSLDYPNLIEPGAEVATVATGLVLLATTPKDDPDSAARVSRFVETVFPRFAE
                     LQSQGRHPKWREVNLAASLPGFKRNAAAASWLLGEHREPKGPVTANADASQAPALPPS
                     PSMSEEQKEALFKRFIEWQRGSER"
     gene            2310..3785
                     /locus_tag="VFY74_03705"
     CDS             2310..3785
                     /locus_tag="VFY74_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930847.1"
                     /translation="MTGSTTPMSEQPDWHPVHRANALLREALQNTTRSFESLEARYSV
                     ESLSDAIESVLQGPVRITYPLPLAVRLHHLSIEEKAIPGLEALMRPSKHAPGDPLAGF
                     RHDGDDGLAAFGQDDGLTALPPEFSDDAEGCPDLDEFATLRADVPLDAEMGGRQVHMQ
                     TLMGEFHRRQRDASRLVGASIATAIVLTIGGVVLAVSLATPRPADAGQLDTERSTSIT
                     WQRPASGTAAGGVQPAVEAANRTAKGEPVSVPHDAGTSRPPVTAVSSASHILLAASGR
                     QIAFGPLLPPSNARYLMIRGLPPEVMLSAGRQSGGGAWLVKGEHVPGLTLTVGRAAAG
                     DHPVEVYILESGDGPQARRSFVLRIAPTDRTFAPAGTPNLISAAAPAAAATPAVEEPT
                     VPAETAVLRDRATRLLGEGDIAAARLLLLHLAERGDGEAAYDLARTFDREMLAELGAK
                     GMDGDAARARGWYERASEDGNVKAAERLKVLASLSGTGPSD"
     gene            complement(4150..6072)
                     /gene="cysN"
                     /locus_tag="VFY74_03710"
     CDS             complement(4150..6072)
                     /gene="cysN"
                     /locus_tag="VFY74_03710"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX5930848.1"
                     /translation="MARLSVVGGSAETDARARVEARIAAEEDRGLLRFLTCGSVDDGK
                     STLIGRLLYDSHLIFEDQIQAAEHGQRRAGAGRGGPLDLALLIDGLQAEREQKITIDV
                     AYRYFSTPRRKFIVADTPGHVQYTRNMATGASNCDFAVLLVDARGGILTQTRRHACIL
                     SLLGIDKVALTINKMDLVDFDQTRFEAIAAEFVAFARELGLAQVTAIPVSALKGDNVV
                     TPSAHMHWYRGPTLLGHLETVDAGPKVGERPFRFPVQWVNRPHLDFRGYAGTVASGRI
                     SPGEEVAIYPSGKRAHVARIVTADGDLETAEQGDAVTLTFAEEVDVSRGDVIALAANA
                     PSVADQMAARIIWFDEEPMLPGRAYTLKCGNQTTTATVLNLKYKLNVDNLDHVAGKTL
                     EINEVGSCNLNTAKPLVFDPYAENIDTGSFILIDRFTNATVAAGMIEFGLRRATNVRW
                     QELSIDKAARSGLKEQKPCVLWFTGLSGAGKSTIANALEKRLHAMGRHTYLLDGDNVR
                     HGLNKDLGFTDADRVENIRRVAEAARLFVDAGLIVMVSFISPFRSERRMARDLFDAGE
                     FIEIFVDTPLEVCEARDPKGLYVKARAGLIANFTGIDSVYEPPERAELKLDASKASSE
                     SLAEAVLAELRQRHLI"
     gene            complement(6072..6980)
                     /gene="cysD"
                     /locus_tag="VFY74_03715"
     CDS             complement(6072..6980)
                     /gene="cysD"
                     /locus_tag="VFY74_03715"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX5930849.1"
                     /translation="MKQRLSTYLRQLEAESIHIMREVAAEFRNPVMLYSIGKDSSVML
                     HLALKAFYPGKPPFPLLHIDTTWKFREMIAFRDREAQRLGVHLLVHINEQGVKDGISP
                     ITHGSALHTDIMKTEALKQALAAHGFDAAFGGARRDEEKSRAKERVFSFRNAAQNWDP
                     KRQRPELWHIANTKIGPGESMRVFPLSNWTELDVWTYIHLENIPVVPLYFAADRPVVE
                     RGGTLIMVDDERLPLDPGEKPEMVRVRFRTLGCYPLTGAIRSEAATVPEIIEELIAAR
                     YSERQGRVIDHDQTGSMEKKKQEGYF"
     gene            complement(6977..7861)
                     /gene="pssA"
                     /locus_tag="VFY74_03720"
     CDS             complement(6977..7861)
                     /gene="pssA"
                     /locus_tag="VFY74_03720"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX5930850.1"
                     /translation="MFPPFDPERDERKRRHTVFTRGQVPVRVLIPSIFTLVGLCAGLT
                     AIRMGIEHRWDLAVAALVFAALLDGIDGRVARLLKASSRFGAELDSLADFVNFGVAPA
                     IIMFNWALEDLHSLGWIAVLVFAVCAALRLARFNVSLDATDTPAWKSNYFVGVPAPAA
                     AIILLLPIYVQDLGLHLPSLTPLVLIYTLAIALLMVSKVPTFSGKLIGQRIAREYVPP
                     IFVLAAAFMAVLLTYPALTLAVGSVTYLAIIPVSAYRYFAEERKAAKLGKTQGPKDQA
                     EEQPARSDDQVIRLRTKP"
     gene            complement(7866..8603)
                     /locus_tag="VFY74_03725"
     CDS             complement(7866..8603)
                     /locus_tag="VFY74_03725"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX5930851.1"
                     /translation="MNTPHSLLDSILGAFVPVHADGFKFVAGAALATLILFALAPPLG
                     WLGALATAYCAYFFRDPPRVTPSRAGLIVSGADGKIVAIGPTVPPRELRLGGEPLARI
                     SVFLSVFDVHLVRAPIAGRIVTSAYTPGAFLNAELDKASEENERHALVIEAPTGERVG
                     IVLIAGLIARRIVTFAGEGRSVARGERIGLIRFGSRVDVYLPAGAETRVEIGQTAIGG
                     ETVLAEVAPAKEARPLSSVIGSSAAEA"
     gene            complement(8622..10556)
                     /locus_tag="VFY74_03730"
     CDS             complement(8622..10556)
                     /locus_tag="VFY74_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX5930852.1"
                     /translation="MQDRLPQSRTDDLLRARAGHWSVLRELLPYIWPDNRADLRWRVV
                     LALAALVVAKIVTLAVPIAYKTVVDILTGNASGETLPGLSAVGLAAIPAVLIIAYGVG
                     RVMMVLFAQLRDVWFTVVAQNAVRQLANRTFRHLHALSLRFHLERRTGGLNRIIERGV
                     GGVDTIVRMAVLNSIPTAVELVMIAGLVAYYFGWIYVVVVLLTVALYVWFTFWASERR
                     IVIRRDMNESDTEAHAKAVDSLLNYETVKYFGNEELEARRFDSSMARYERAAVRTYTS
                     LGLLNSGQAVIFSIGTVICMLLAARDVTRGALTVGGFVMINAILIQLYVPLNFMGMVY
                     REIKQGLIDIETMFALLHEPAEIVDRPDAKPLRVRKGEIKFENVAFAYDAERPILKHL
                     SFTVPAGNMVAIVGPSGAGKSTISRILFRFYELTGGRVLIDGQNIRDVTQASLRAAIG
                     MVPQDTVLFNDTIEYNIRYGKPDASPAEVREAARLAQIDDLIMTLPQGYDSLVGERGL
                     KLSGGEKQRVAIARTILKAPPILMLDEATSALDSHTEREIQDALERVARERTSLVIAH
                     RLSTVVHADNIIVLDRGEIVEQGKHLELLAKGGLYASLWARQRQADEARERLAHALEE
                     DALPPSRAAERLEDEALAST"
     gene            11167..12009
                     /locus_tag="VFY74_03735"
     CDS             11167..12009
                     /locus_tag="VFY74_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5930853.1"
                     /translation="MRVLAHHPGSRCTVGMAFRMTRGSHDLIGKVYAEDRSDVYQALE
                     RIKRAGFGPEAEYAIPDPIHYVAGLRLLLLEKAHGPLAQVLFLKGNEKDRVVASERCG
                     RWLARFQALAPKAGPVLELNRHLIRLERWSRRIAKFGEPSAGKAARLFERLEAAASKC
                     TGMEMTAGHASYSPSHIILGPARTVTFDWDGYDVAHPLRDVARFMIALRRLALGGLRS
                     IRALDAEAELFLSAYMDAKRAGEAADLPFFQAATCLQLAVYNLSHQGRERIDAMLDEG
                     LRIL"
     gene            complement(12169..13443)
                     /locus_tag="VFY74_03740"
     CDS             complement(12169..13443)
                     /locus_tag="VFY74_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5930854.1"
                     /translation="MVGRIAIMVAIGVGLLAAAAVFYLFSQGALPRFETANKTPAGTV
                     APAAPKASPPQTAALPEPGGAPKAKEEATGVTTPARPTVVPSFDVVVIEPSGEGVIAG
                     RAAPGWQVDVESGGTKVAGATADDQGEWSAVLERPLPAGDHALSLKITSPDGTRALSS
                     QERVRVEVGGTTRVAAAPPQAAASSDAFPGGAPAAPPAAEQNVAPQEQAPDLALAEPR
                     ATRAPDVPADETRATARAVSPDAEESKAVPSAPKLVFKTVDYNDKGTSSGSVSITGTS
                     DPGATIKVYSGDELLATVRADPEGSWSVMAEKTLPTGKHNFRAERIDTATGVPSAQAM
                     VAIERLVPKPPEVAAMQTKGAAPTAKDAASETALRSKDVYTVKRGDTLWAIAKRYFGS
                     GLRYPTIFQDNREIITDPDLIHPQQEVKVPPE"
     gene            complement(13481..13960)
                     /locus_tag="VFY74_03745"
     CDS             complement(13481..13960)
                     /locus_tag="VFY74_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930855.1"
                     /translation="MTCNCIRIVTVAIGLAALGLGAAFAGGGAGLPEGAASAAEGSET
                     ASPPPRSPLRLSTIDFQDRGAGSGKLTLAGVALPGSELHLFLDDEPFATVTPDGGGKW
                     SYEGAMTLGDGRHTLRADQYDQATNMLAARAMITIQRAKPGAGDAPPAAPAPTAASP"
     gene            14130..14744
                     /locus_tag="VFY74_03750"
     CDS             14130..14744
                     /locus_tag="VFY74_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5930856.1"
                     /translation="MNETHITPGLQNVCVYCGSGTGRNPAYVEAARSLGRILAAARIG
                     LVYGGGSLGLMGEIARSVLDHGGHVTGIIPGFLSEREHMLTEAQELIVVDDMHQRKHL
                     MFAKSDAFVALPGGLGTLEEFVEQLTWSQLGRHRKPIVLANIEGFWDPLLVQFEGMES
                     ESFIRPGLDLNLLVVERVEDIVPAIERAIGLPAARAEAEVAAKF"
     gene            complement(14780..15538)
                     /locus_tag="VFY74_03755"
     CDS             complement(14780..15538)
                     /locus_tag="VFY74_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930857.1"
                     /translation="MRRFLVFLLLLLWCTPALPQASEGSPYHGNSAPSDRANEGHALD
                     SGKAAQAQEAGANADCVRGEGVPGTAVYYACRARMIFFGSIERGLVVLTALLALSTLL
                     LWIYTRRVANAARAAAEHVPAVERAYVFGGPTDLFLLHDQATVRLAMQNYGKTPAVIR
                     EWLVEFLAQEPRGRKPAYDGAKRTLTNEILEPFKLYSPATVFRSEIPAPFYIVGYIVY
                     DDVFRRSHTTRFCVGVARDGKAAFGGHPEWNAYD"
     gene            complement(15654..17255)
                     /gene="cimA"
                     /locus_tag="VFY74_03760"
     CDS             complement(15654..17255)
                     /gene="cimA"
                     /locus_tag="VFY74_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX5930858.1"
                     /translation="MTRERLSLYDTTLRDGAQTHGVDFSVEDKWLIAEQLDALGIDYI
                     EAGYPGANPTDTRLFAEARPLGRANLTAFGMTKRPGRSIANDPGFQALLAAKVSSICL
                     VAKSWDFHVEVALGISLAENLAGIKESTEAIVASKREALIDCEHFFDGYKANSAYALA
                     CAQTAYDAGARWVVLCDTNGGTLPEEIERIVTEIVKHIPGDHLGIHVHNDTDNGVANS
                     LAAVRAGARQIQGTLNGLGERCGNANLTSIIPTLLLKPDYAARFETGVTADKLKTLSH
                     ASRVLDEVLNRAPNRRAPYVGESAFAHKGGIHVSAVQKDPRTYEHVPPEAVGNRRKLL
                     VSDQAGRSNILAELERIGIAVPKDDPRIARLLEEVKQREANGYAYEAAEASFELLARR
                     TLGNVPQFFEVDSFRVLVERRHNALGELVTISEATVKVRIDGETIMSVGEGNGPVNAL
                     DNALRKDLGRYQGFIEDLDLVDYKVRILTGGSDAVTRVLIESRDRVGKRWFTVGVSPN
                     IVDASFEALLDSIHYKLIRDAAPVP"
BASE COUNT         3007 a         5594 c         5641 g         3088 t
ORIGIN      
        1 aggtggcggt gagcttcgaa gccaagctgg tggtctatgg cgatcccgac gacgcgccgc
       61 tgccgaggaa gtttctcatc aagccggaga accggctcat cgtgcgcgat gcgaacggca
      121 agacggcggt cgacgaaccg ctctgcggca actgcaccga cattttcgac gacaaggtga
      181 gcatcgtgct cccgctcgcc gtcgcgcccc tcctccgcga gaacaagacc atcgacatgg
      241 cggtcgggct ggccggcaag gaggaatgcc gcttcaagct cgacggcgag agcttgcgcg
      301 ccgggcttga ctgggcatcc gaacgccgga cggcgctggc ggaagcccgt gcggaggaga
      361 aatgcactcc gcccgaaggc tgcttcatca cgtcggcctg ttgcgaggcg atcggactcg
      421 acgacaattg cttcgaactc agggctctca ggcgctatcg cgacgaggtg cttgcgaagc
      481 ggccgggggg cgtgaacgag atcgccgcct attacgcgct cgcaccgtcg atcttggggc
      541 gggtcccgcg caagggccgg aaacgcctgc tgcgtttcct ctatgtccgc tacatccttc
      601 cgagcgcgct ggcggcgacg tttggcttcg acgccctcgc ctataggctc tatgcgcgga
      661 tgctccgtga gctcactctg gcctttgcgg ccgaggagat gccgtcatcc tgaggtgcga
      721 gcgaagcgag cctcgaaggg tgacgccgtg caaaggccgg tccctcgagg gccgcctgcg
      781 gcggccacct caggatgacg tgccgcgcaa ccaggccccg gctttgccgc attcaggccg
      841 atttggcgcc ttactcgatc gttaaccttc acccaacaag aaaaaggcct tctgctccgg
      901 atttccgggg ccccctcgcg caaagcataa gaccgcatac caggagtttt tcgctcccgg
      961 cctcggcgat gaagacggcg actgtgtccg cgccaacgct tcatctcggg ctcggggcaa
     1021 aaaagtgttt cgcctcggga acttctcccg cacggcgtgc gtatttgcgg ggatacgggg
     1081 gtttcgtgtt gcgtaccgtt tgggttgttg gcctcgttct cgcatggctt gttgggagcg
     1141 gccccgttca gggtgccgac aacaagagca atgccgatca agcctggctt ggactgatcg
     1201 cgggctccgc cgccggttcg gagaccgtgc tcgccgccga tatggcgagc ctcttcccgc
     1261 aaggcgattt gggcatcctc ccgatcttgg gcgatgccgg cgccggcaat ctcgctcgtc
     1321 tcgaagacct taaggtcgat atcgcttttg tctcgaccga cgtcttggcg gaggcggcag
     1381 ccaaggacga cagcttgcgc aaccggctcg aactcgtgac gcgtcttgcc ccgcaggagg
     1441 ttcatgtgct ggcccgcgcg gatatcggca aactctccga cctgtcgggc cgtgaggtga
     1501 atttcggccc gccagggtcg gcgtcggcgg tgacagctgc cgcgctcttc aaggcgcttc
     1561 ccgtcgaggt cgaggctttg agcctggaca cgacaacggc aatcgagcgc ttgaaggaag
     1621 gcgccatcgc cgccgcggtc gtcgtcggcg gcaagccgtc gcccgtggtc ggcgccattc
     1681 cgcccaattc cggcatccat ctgcttcccg tctcgttcgg cgctaccttg gcaacggaat
     1741 atctgccaac ccggctcggg tctctcgact atcccaatct catcgaacct ggcgccgagg
     1801 tggcgacggt cgcgaccggt ctcgtcctgc tcgcgacaac tccgaaagac gatccagatt
     1861 ctgccgcgcg cgtttcccgt ttcgtcgaga cggtcttccc gcgcttcgcg gaactccagt
     1921 cgcaaggccg gcatcccaaa tggcgcgagg tgaatctcgc cgcgtcactg ccgggcttca
     1981 aacggaacgc cgccgccgct tcatggctcc ttggcgagca tcgtgaacca aaggggccgg
     2041 tgacggcaaa cgccgacgcg agccaggctc cggcgcttcc cccaagtccc tccatgagcg
     2101 aggagcaaaa ggaagcgttg ttcaagcggt tcattgaatg gcagcgaggc agcgagcgct
     2161 gacgcgcctt gagggagagg gaggaaagag aagaagcagt ttgcggttcg gtacatcggc
     2221 gctcgcgagg caggagcgcg tcttgacggg ggaaagtggg gcgtagcgcg cgcggaggta
     2281 ttcgcgcggg cgaatgagga gtaacgagta tgaccggttc cacgacgccc atgtcggagc
     2341 agcccgattg gcatccagtc catcgcgcca atgcgctgct acgcgaagct ttgcagaata
     2401 cgacgcggtc cttcgagtca ttggaggctc gctatagcgt cgagtcgctg tcggacgcga
     2461 tcgaaagcgt acttcaaggg ccggtgcgta ttacctatcc attgccgctg gcggtgcgcc
     2521 tccaccactt gtcgatcgaa gagaaagcga tcccgggact agaggctctc atgcgcccct
     2581 cgaagcatgc tcccggcgat cccttggccg gtttccggca tgacggcgat gacggcttgg
     2641 cggcgttcgg acaagacgac ggactcaccg ccctccctcc ggaattctct gatgatgcgg
     2701 agggctgccc ggaccttgac gagttcgcaa cgcttcgggc cgatgtgccg cttgacgccg
     2761 aaatgggcgg tcggcaggtc catatgcaga cgctgatggg agagttccat cgccgccagc
     2821 gcgatgcgag ccggctcgtt ggcgcgtcca tcgcgaccgc aattgtgctc accattgggg
     2881 gcgtcgtgct cgccgtgagc ctcgcaactc cccgtcccgc cgacgcgggc caactcgaca
     2941 cagagcgttc cacctcgatc acctggcaga gaccggcctc cggcacagcc gccggcggcg
     3001 ttcagcccgc ggtcgaggcg gccaatcgca ccgccaaggg cgaaccggtt tccgttccgc
     3061 acgacgccgg cacttcgcgc ccgccggtta cagccgtgtc atcggcgtcg cacatccttc
     3121 ttgccgcaag cggccggcag atcgcatttg gcccgcttct gccgccgagc aacgcccgct
     3181 atctcatgat ccgcggactt ccgcccgagg tgatgttgtc ggccgggcgc cagagcggag
     3241 gaggagcatg gctggtcaag ggtgagcatg tgcccggcct gaccctcacc gtcggccgcg
     3301 cggccgccgg cgaccatccg gtcgaagtct atattctcga gtcgggcgat gggccgcagg
     3361 cgcgccgaag cttcgtgttg cggatcgccc cgaccgaccg cacttttgcg ccggcgggaa
     3421 caccgaattt gatttctgcc gcagcccctg ccgcggcggc cactcccgcc gtcgaagagc
     3481 ccactgttcc ggccgaaacc gccgtgctgc gcgacagggc cacgcggctt ctcggcgagg
     3541 gcgacatcgc cgcggcgcgg ctgctgctgc tccacctcgc cgaacgcggc gacggcgagg
     3601 cggcctacga tcttgcgcgc accttcgacc gcgagatgct ggccgagctc ggcgccaagg
     3661 gaatggacgg cgatgccgca cgcgcccgcg gctggtatga gcgcgcctcc gaggatggca
     3721 acgtcaaggc tgccgaacgg ttgaaagtcc tcgctagtct ttcgggaacc ggtccctccg
     3781 actagcgcga ggagggccgc agccgctcat ccgagggaaa ccaatcgggg ggagtagagc
     3841 agttgcggcc cactcacttt aattttgagg caccgcactc gcgtccatct tcaaacctcc
     3901 ccctcgtggg gaggtccgac gacgccgtta cttatgcgct accgctccgc tgcttgagcg
     3961 caaggcggcg tcggggtgcg gggcgtcgct ctcaatcggc aaccacgtca ttccgggccg
     4021 aagccggaat ccagaagaaa catgcactgc gtggtgagcc tggataccgg ctttcgccgg
     4081 tatgacgaaa atcacctcac gcggctcggc cttcggcctc gccgccctcc ccatcaaggg
     4141 gagggtagat catatgaggt gacgctgcct gagctcggcg agcacggcct cggcgagcga
     4201 ttcggacgac gccttgctgg catcgagctt gagctccgcc cgctcgggcg gttcgtacac
     4261 ggaatcgatg ccggtgaaat tggcgatcag tccggcgcgt gccttgacgt agagaccctt
     4321 ggggtcgcgc gcctcgcaca cctccagcgg cgtatccacg aagatctcga tgaactcgcc
     4381 cgcgtcgaac aggtcgcgcg ccatgcgccg ctccgaacgg aagggcgaga tgaacgacac
     4441 catgacgatg agaccggcat cgacgaagag ccgcgcggct tcggccacgc ggcggatatt
     4501 ctccacacgg tctgcgtcgg tgaagccgag atccttgttc aggccgtggc ggacattgtc
     4561 gccatcgagc agataagtgt gtcggcccat ggcgtgcagc cgtttttcca gcgcattggc
     4621 gatggtcgat ttgccggcgc cggaaagccc ggtgaaccac aacacgcagg gcttctgctc
     4681 cttgagtccg gaccgcgccg ccttgtcgat ggacagctcc tgccagcgca cgttggtggc
     4741 gcggcgcagg ccgaactcga tcatccccgc cgcgacggtg gcgttggtga aacggtcgat
     4801 caggatgaac gagccggtat cgatgttctc ggcgtaggga tcgaacacca gcggcttggc
     4861 ggtgttgagg ttgcaggacc caacctcgtt gatctcgagc gttttgccgg cgacatggtc
     4921 gagattgtcg acgttgagct tgtatttcag gttgagcacg gtcgccgtcg tggtctggtt
     4981 cccgcatttg agcgtatagg cgcgcccggg cagcatcggc tcctcgtcga accagatgat
     5041 gcgcgcggcc atctgatcgg ccaccgaagg cgcgttcgcc gcgagcgcga tgacgtcgcc
     5101 gcggctgaca tcgacctcct cggcaaaggt caaggtcacg gcgtcgcctt gctccgccgt
     5161 ttcgaggtct ccgtcggcgg tgacgatccg cgccacatgg gcccgcttgc ctgagggata
     5221 gatcgcgacc tcctcgccgg gcgagatcct gccgcttgcc accgttccgg catagccgcg
     5281 aaaatcgaga tgcggccggt tcacccactg cacgggaaac cggaacggcc gctcgcccac
     5341 cttcggcccg gcgtccaccg tctcgagatg cccaagcagc gtcggcccgc gataccagtg
     5401 catatgcgcg ctgggcgtga cgacgttgtc gcccttgagc gcggacacag ggatagccgt
     5461 gacctgtgcg agaccgagct cccgcgcgaa cgcaacgaac tccgcggcga tcgcctcgaa
     5521 gcgggtctga tcgaaatcga cgagatccat cttgttgatg gtgagcgcga ccttgtcgat
     5581 gccgagaagc gataggatgc aggcatgccg ccgggtctgg gtgaggattc cgccgcgggc
     5641 atcgaccagg agcacggcga agtcgcaatt cgacgcacct gttgccatgt tgcgcgtgta
     5701 ctgcacgtgg cccggcgtat cggcgacgat gaattttcgc cgcggcgtcg agaaatagcg
     5761 gtaagccacg tcgatggtga tcttctgctc gcgctcggcc tgcaatccgt cgatcagaag
     5821 cgcaaggtcg agcggccctc cccttcccgc gccggcacgg cgctggccgt gctccgccgc
     5881 ctggatctga tcctcgaaga tcagatgcga atcgtaaagc aggcggccga tcagcgtgct
     5941 cttgccgtca tcgacgctgc cgcaggtgag aaagcgcaat aggccgcgat cctcctccgc
     6001 cgcgatccgg gcttcgaccc gcgcgcgcgc gtccgtctct gcgctgccgc cgacgacact
     6061 cagccgcgcc atcagaaata gccctcctgc ttcttcttct ccatcgatcc ggtctgatcg
     6121 tggtcgatga ctcggccttg gcgttccgaa tagcgcgcgg cgatcagctc ctcgatgatt
     6181 tccggcaccg tcgcggcctc cgaccggatc gccccggtga gagggtagca gccgagcgtg
     6241 cggaagcgga cacgcaccat ctcgggcttc tcgccgggat cgagcgggag tcgctcgtcg
     6301 tcgaccatga tcagcgtgcc gccccgctcg accacggggc gatcggccgc gaaatagagc
     6361 ggaacgacag gaatgttctc caggtggata taggtccaca catcgagctc ggtccaattg
     6421 gacagcggga acacccgcat gctttcgccg ggaccgatct tggtgttggc gatatgccac
     6481 aactccggcc gctgccgctt ggggtcccag ttctgcgccg cattgcggaa cgagaatacg
     6541 cgctctttcg cccgcgattt ctcctcgtcg cgccgcgccc cgccgaaggc cgcatcgaaa
     6601 ccgtgggcgg cgagcgcctg cttgagcgcc tccgtcttca tgatgtcggt atggagcgcg
     6661 ctgccatggg tgatgggcga gatgccgtcc ttgacccctt gctcgttgat atgcacgagg
     6721 agatggacgc cgagccgctg cgcctccctg tcgcgaaagg cgatcatctc gcggaacttc
     6781 cacgtggtgt cgatgtggag cagcggaaac ggcggcttcc ccggatagaa tgccttgagc
     6841 gcgagatgca gcatcaccga ggaatccttg ccgatcgagt acagcatcac gggattgcga
     6901 aactcggccg cgacctcgcg catgatatgg atgctctccg cctcgagctg gcggagatag
     6961 gtggacagcc gctgcttcat ggcttggttc tcagccggat cacttgatcg tcggaccgtg
     7021 cgggctgttc ctcggcctga tctttcgggc cttgtgtctt tcccaacttc gccgccttgc
     7081 gttcctcggc gaaatagcga taggcggaaa ccgggatgat cgcgagataa gtcaccgaac
     7141 cgaccgcgag cgtcagggcg ggatatgtca ggagcactgc catgaaagcc gccgcgagca
     7201 cgaagatcgg cggcacatac tcgcgcgcga tccgctgccc gatcagcttg ccggagaagg
     7261 tgggcacctt gctcaccatg agaagcgcga tcgcgagcgt atagatcagg acgagcgggg
     7321 tgaggctcgg caaatggagc ccaagatcct gcacgtagat gggcaacaag agaatgatgg
     7381 ccgcggccgg cgccggcacg ccgacgaagt agttgctctt ccaggcgggc gtgtcggtgg
     7441 cgtcgagcga cacgttgaaa cgcgcaagcc ggagcgcggc gcacaccgcg aacacgagga
     7501 ccgcgatcca accgaggctg tgcaggtctt ccagcgccca attgaacatg atgatggccg
     7561 gcgccacgcc gaaattgacg aagtcggcca agctgtcgag ctcggcgccg aaccgcgagg
     7621 aggccttgag caaacgcgcc acgcgcccgt cgatcccgtc gagcagagcc gcgaacacga
     7681 gcgccgccac cgccaggtcc cagcgatgct cgatgcccat gcgaattgcg gtcagcccgg
     7741 cgcagagccc gacgagagtg aagatgctcg ggatcagcac cctgaccggc acttggccac
     7801 gcgtgaagac cgtgtgccgc cgcttgcgtt cgtcgcgttc gggatcgaaa ggaggaaaca
     7861 tggctttaag cctccgccgc gctcgagcct atcacagaac tcaacggccg cgcctctttg
     7921 gcgggcgcga cttctgccag caccgtctct ccgccgatcg ccgtttgacc gatttcgacg
     7981 cgggtctcgg cgccggcggg caaatacaca tcgacccggc tgccgaagcg gatgagaccg
     8041 atgcgctcgc ctctggccac gctacgcccc tcgcccgcga aagtcacgat gcggcgcgcg
     8101 atcaatccgg cgatcagcac gattcccacc ctctcgccgg tcggcgcctc gatcacgaga
     8161 gcgtggcgct cgttctcctc gctcgccttg tcgagctcgg cgttgaggaa ggcgcccggc
     8221 gtataggcgc tggtcacgat gcggccggcg atgggagcgc gcacgagatg cacgtcgaag
     8281 accgagagga acactgagat gcgtgcgagc ggttcacctc cgagccgaag ctcgcgtggc
     8341 ggcaccgtgg ggccgatggc gacgatcttg ccgtcggcgc cgctcacgat caggcccgct
     8401 cgggacggcg tcacgcgcgg aggatcgcgg aagaaatagg cgcaataggc cgtggcgagg
     8461 gcgccgagcc agccgagtgg cggggcgagc gcgaagagaa tgagtgtcgc gagcgcggcg
     8521 cccgcgacaa atttgaagcc gtccgcatgc accgggacaa aggcgcccag gatgctgtcg
     8581 aggaggctgt gaggtgtgtt catgccggcg cgattcgcaa gttacgtcga ggcgagagcc
     8641 tcgtcctcga ggcgctcggc cgcgcgcgag ggcggcagcg cgtcctcttc gagcgcgtgc
     8701 gcgagccgct cgcgcgcctc gtcggcctgc cgctggcgcg cccacaggct ggcatagagg
     8761 ccgcctttgg cgagaagctc aaggtgcttg ccctgctcga cgatttctcc gcgatcgaga
     8821 acgatgatgt tgtcggcgtg cacgacggtt gagaggcgat gggcgatgac gaggctcgtg
     8881 cgctcccgcg cgacgcgctc gagcgcgtcc tggatctcgc gctcggtatg actgtcgagc
     8941 gccgaggtcg cctcgtcgag catcaggatc ggcggcgcct tcagaatcgt gcgggcgatg
     9001 gccacgcgct gcttctcgcc gccggagagt ttcaggccgc gttcgccgac cagtgaatcg
     9061 tagccttgcg gcagcgtcat gatcaaatcg tcgatctggg cgagccgcgc cgcctcgcgc
     9121 acctcggccg ggctcgcatc gggcttgccg tagcggatgt tgtactcgat cgtgtcgttg
     9181 aacagcacgg tgtcctgcgg caccatgccg atcgccgccc tgagcgaggc ttgcgtcacg
     9241 tctctgatgt tctggccgtc gatcagcacc cgcccgccgg tcagctcata gaaacggaag
     9301 agaatgcgcg agatggtgga tttacccgcg cccgaaggtc cgacgatggc gaccatgttt
     9361 cccgccggca ccgtgaagct caaatgcttc aggatcgggc gctcggcatc gtaggcaaag
     9421 gcaacgttct cgaatttgat ctcgcccttc ctcacgcgga gcggcttggc gtccggccgg
     9481 tcgacgatct cggccggctc gtgcaggagc gcgaacatgg tctcgatgtc gatcagcccc
     9541 tgcttgatct cgcgatagac catgcccatg aagttgagcg gcacgtaaag ctggatgagg
     9601 atcgcattga tcatgacgaa accgccgacg gtcagcgcgc cgcgcgtgac gtcgcgcgcc
     9661 gccagcagca tgcagatcac cgttccgatg gagaagatca cagcctgtcc ggaattgagc
     9721 aggccaagcg aggtataggt gcggaccgcc gcgcgctcgt agcgcgccat cgaggaatcg
     9781 aaccgccgcg cttcgagctc ctcgttgccg aaatatttta ccgtctcgta gttgagcaga
     9841 ctgtcgaccg ccttggcatg cgcctcggtg tcggactcgt tcatgtcgcg gcggatgacg
     9901 atgcgccgtt cgctcgccca gaaggtgaac cagacgtaga gggcgacggt gagcagcacg
     9961 accacgacgt agatccagcc gaaatagtag gcgaccagcc ccgcgatcat gacgagctcg
    10021 accgcggtcg ggatcgaatt caacaccgcc atgcgcacga tggtgtcgac gccgccgacg
    10081 ccgcgttcga tgatgcggtt caagcccccg gtgcggcgct cgagatgaaa gcgcaaggag
    10141 agcgcgtgca gatgccggaa ggtcctgttg gcgagctggc ggaccgcgtt ctgggcgacc
    10201 acggtgaacc atacgtcgcg gagctgggcg aacagcacca tcatcacgcg tccgacgcca
    10261 taggcgatga tgagcacggc cgggatggcg gcgagcccga ccgcagaaag accgggaagc
    10321 gtctcgccgc tcgcgtttcc tgtcagaata tcgacgaccg tcttgtatgc gatcgggacc
    10381 gcaagcgtga cgatcttggc gaccacgagc gccgccagcg ccaagacaac gcgccatcgg
    10441 agatcggcgc ggttgtccgg ccagatatag ggcaacagct cgcggagcac gctccagtgc
    10501 cccgcccgcg ctcgaagcag gtcgtcggtt ctggattgcg gcagccggtc ttgcataaga
    10561 cttcgtttct atcagcgcgc gctcggcgcg gcaaaaggag cagcgaccgg acgcagctaa
    10621 agcaggcacc ggttagccac ggctaaacct tttttgccat cctaaccgat tgtaaagtca
    10681 aaaataattg acgtagcctg cccccgttta gccccggctg accccgtatg cattggcagc
    10741 aaagccggga gttgaaccgg ttcaacatag gaggcaaaga tggctatcgt cgatgagttc
    10801 acacccgcct tgaggctcaa ggctgggagc atacgcgtca gcagcttttt tcgccgccgc
    10861 atttagccga accgaaatca cctctgcgac tggtttggaa caacccgagt acgcctgtat
    10921 tctcttctgg ggaggactcc atacgcctca ctggatagga atttagtgcc taagcggacc
    10981 gaataatgcg tcggcccgat tgttttcacg tcggctacga ggattgaggt ggcgtccaga
    11041 ccggtaccag ctgtcgacga ccccggaatt ccgactctgg cggcggtgct ccagcctcat
    11101 gaactaaacc ggcatcttcg ggcaactctg cccgttgaat gggggacatt ggaagaggtg
    11161 cggcgagtgc gggtgcttgc acatcatccc ggcagccgct gcaccgtcgg gatggctttt
    11221 cgaatgacac gtggctcgca tgatttgatc ggaaaggtct acgccgaaga ccgatcggac
    11281 gtttatcaag cgctagagcg aataaagcga gccggctttg gtcctgaggc agaatatgcc
    11341 atcccagatc cgatccacta tgttgccggc ttgaggctcc tgcttctgga aaaggcgcac
    11401 ggccctctag cgcaggtcct gtttctgaaa gggaacgaaa aggatcgcgt cgttgcctca
    11461 gaacgctgcg gccgatggct agctcggttc caggcactcg ctccaaaggc agggccggtg
    11521 cttgagctta accgtcacct gattcgtttg gagcgatggt cgaggcgtat agcgaaattc
    11581 ggcgaaccct ctgccggcaa agcagcccgc ttgttcgagc ggttagaagc tgccgcttcg
    11641 aaatgcaccg gaatggaaat gactgcgggg cacgcaagtt atagcccttc ccacatcatc
    11701 ctaggtccgg cacgtactgt tacgttcgat tgggacggtt acgatgtggc gcatcccctc
    11761 cgcgacgtgg cccgattcat gatcgctctc cggcgcctcg cgctgggcgg gctccggtcg
    11821 atccgtgcat tggacgccga agccgagttg tttctgtcgg cctatatgga cgcgaagcgc
    11881 gcgggcgagg cggcggacct tcctttcttt caggctgcca cgtgtctcca gctcgccgta
    11941 tacaacctct cgcaccaggg gagggagaga attgacgcca tgctggacga agggcttcgg
    12001 attttgtagt tgtaaccgtc tgcgggtggt gcccaccccg tcaaagaatg gcagcatcaa
    12061 taggtggatt gcgccccaag gcgtagcccg gttgctacgc ccgcgcccgt gatgtgacgc
    12121 ctcgcccctg ttcttcggac tatataggcg cccgccccgc caagcgcgtc attctggcgg
    12181 caccttcacc tcctgctgag gatggatcag gtcgggatcg gtgatgatct cgcggttgtc
    12241 ctggaaaatc gtggggtagc gcaggccgct gccgaaatag cgcttggcga tggcccacag
    12301 cgtatcgccg cgcttgacgg tatagacgtc cttcgaccgc aaagcggtct cggaagccgc
    12361 atctttggct gtgggcgccg ctcccttcgt ttgcatggcg gcgacctccg gcggcttcgg
    12421 gacgagacgc tcgatcgcga ccatcgcttg ggccgagggt acgcccgttg ccgtatcgat
    12481 gcgctcggcg cggaaattgt gtttcccggt gggaagcgtc ttctccgcca tgacgctcca
    12541 gcttccctcg ggatcggcgc gcaccgtggc gagcagttcg tcccccgaat agaccttgat
    12601 cgtcgccccc ggatcgctcg tgccggtgat cgacacgctg cccgaggatg tgcccttgtc
    12661 gttgtagtcg accgtcttga agacgagttt cggcgccgaa ggcacggcct tgctctcttc
    12721 ggcgtcggga gaaacggcgc gcgcggtcgc tcgcgtctcg tccgcgggca cgtccggcgc
    12781 ccgcgtggct ctcggctcgg ctagtgccag atccggcgcc tgctcttgcg gcgccacatt
    12841 ttgctcggcc gccggtggcg ctgccggcgc cccgcctggg aacgcatccg aactcgccgc
    12901 agcttgcgga ggggcggcgg cgaccctcgt cgtacctccc acttcaacgc gcacccgctc
    12961 ctgcgacgac agagcgcgcg ttccatccgg cgaggtgatt ttcaaggaga gcgcatgatc
    13021 gccggcggga agcggcctct ccaagactgc gctccactcg ccttgatcat cggccgttgc
    13081 ccccgccacc ttcgtgccgc cgctttcgac gtcaacttgc caacccggtg cggcacggcc
    13141 cgcgatgaca ccctccccgc ttggctcgat caccaccaca tcgaagctcg gcacgaccgt
    13201 cggtcgggcc ggtgtcgtga cgccggtcgc ctcctccttt gccttcggcg ccccgccagg
    13261 ctcgggtaaa gcggcagtct gcggcggcga cgccttcggt gccgctggcg cgacggttcc
    13321 cgcaggtgtc ttgttggcgg tctcgaagcg aggcaatgcg ccttggctga aaaggtagaa
    13381 cacagcggca gcggccagaa ggccaacgcc gatggctacc atgatggcga ttctaccgac
    13441 catccgtcat tccttcgccg gcggttcgcg atctcctccg ctagggtgag gcggctgtgg
    13501 gggccggcgc ggccggaggc gcgtcgcccg ctcctggttt ggcgcgctgg atcgtgatca
    13561 tggcccgcgc cgccagcata ttggtggcct ggtcgtattg gtccgcgcgc agagtatgac
    13621 gtccgtcgcc aagggtcatc gcgccctcat agctccattt gccgccgcca tccggcgtga
    13681 cggtggcaaa gggctcgtcg tcgagaaaca gatggagttc gcttccgggc agcgcaaccc
    13741 cggcaagcgt cagcttgccc gagcccgcgc ccctgtcctg aaaatcgatc gtgctgagcc
    13801 tgagcgggga ccgcggcggt ggcgatgccg tttcgctccc ctcggccgct gacgccgcac
    13861 cttcgggcaa cccagccccg ccgcccgcga acgccgcgcc aagcccaagg gccgccagcc
    13921 cgatcgcaac cgtcacgatg cggatacaat tgcacgtcat tcgatcattc ctcactcgac
    13981 tcatgttacg cgcccgccaa ggcaaaaaaa cgtcaaaccg ccccggatct tgccgaaact
    14041 atcgaattcg ccgggtcccc gccatcggaa tgacgggcgg ggcataggct tccttgacct
    14101 tcgcctgacc cgaatgccac atgacatcca tgaatgaaac gcacatcacg cccggcctgc
    14161 aaaacgtctg cgtctattgc gggtccggaa ccgggagaaa cccggcctat gtcgaagcgg
    14221 cgcgaagcct cgggcgcatc ctcgccgccg cgcgcatcgg gcttgtctat ggcggcggca
    14281 gccttggcct catgggcgag atcgcccgtt cggtgctcga tcatggcggc cacgtcaccg
    14341 gcatcattcc gggcttcctc agcgagcgcg agcacatgct gaccgaggcc caggaactca
    14401 tcgtcgtcga cgacatgcat cagcgcaagc acctgatgtt cgccaagtcg gacgccttcg
    14461 tggcccttcc gggcggtctc ggcacgctcg aagagttcgt cgagcagctc acctggtcgc
    14521 agctcggccg ccacaggaaa ccgatcgtgc tcgcgaatat cgagggcttt tgggaccctc
    14581 tcctcgtcca gttcgagggc atggagtccg aaagcttcat tcgccccgga cttgacctca
    14641 acctgctcgt ggtcgagcgc gtggaggaca tcgttccggc gatcgaacgg gcgatcggcc
    14701 ttcccgccgc gcgcgccgag gccgaagttg cggcgaagtt ctgagcctca agagcacgct
    14761 gagcgaagcg gcgcgcgcct cagtcgtaag cgttccactc gggatggcct ccaaaggcgg
    14821 ccttgccgtc gcgtgcgacg ccgacgcaga accgggtcgt gtgcgaacgc cggaacacat
    14881 cgtcatagac gatgtagccg acgatataga acggcgccgg gatctccgac cggaagaccg
    14941 tggccggaga gtacaatttg aacggctcga ggatctcgtt ggtgagggtt cgcttcgccc
    15001 cgtcataggc gggcttccgt ccgcgcggct cctgcgccag gaactcgacc agccattcgc
    15061 ggatcaccgc cggggtcttg ccgtaattct gcatggcgag ccgcaccgtc gcctggtcgt
    15121 gcaacagaaa gaggtcggtc ggaccgccaa agacataggc gcgctcgacg gcgggaacgt
    15181 gctccgcggc ggctctcgcg gcattggcaa cgcgccgcgt atagatccaa agcaagagcg
    15241 tcgaaagcgc aagcaacgcg gtgaggacga caaggccccg ctcgatcgag ccgaagaaaa
    15301 tcattctcgc ccggcaggcg taatagacgg ccgttcccgg cacaccttct cctctgacgc
    15361 aatcggcgtt cgcgccggcc tcttgcgctt gcgcggcctt gcccgaatcg agcgcgtggc
    15421 cttcgttcgc cctgtcagac ggcgcggaat tgccgtgata gggactgccc tcgctcgcct
    15481 gcgggagcgc gggcgtgcac cacaagagca gaagaagaaa gacgagaaac ctccgcatcg
    15541 cgcggtcgca gctcccagcg gtatcggcga ggcattctta tatctgaaat cgcgtgcggc
    15601 caaccgaaag cggcagcgtc gcggcccgct gttccttatt gggacagacg gccttagggc
    15661 accggcgcgg cgtcgcggat gagcttgtag tggatggagt cgagcagagc ctcgaaggag
    15721 gcatcgacga tgttcggcga cacgccgacg gtgaaccagc gctttccgac gcggtcgcgg
    15781 ctctcgatca ggacgcgagt cacggcgtcg ctgccgccgg tcaggatgcg gaccttgtag
    15841 tcgacgaggt cgaggtcctc gatgaagccc tgatagcggc cgaggtcctt cctgagcgcg
    15901 ttgtcgagcg cgttcaccgg gccgttgccc tcgcccacgg acatgatcgt ctcgccgtcg
    15961 atgcgcacct tgacggtcgc ctccgaaatc gtaacgagtt cgccgagcgc gttgtggcgc
    16021 cgctcgacca agacgcggaa cgaatccacc tcgaagaatt gaggcacatt gccgagcgtg
    16081 cgccgcgcga gcagctcgaa ggaggcttcg gccgcctcat aggcgtagcc gtttgcctcg
    16141 cgctgcttca cctcctcgag gaggcgcgcg atcctcgggt cgtccttggg aacggcaatg
    16201 ccgatgcgct cgagctcggc gaggatgttt gagcggccgg cctggtccga gaccaaaagc
    16261 ttgcgccggt tgccgaccgc ctccgggggc acatgctcgt aggtgcgcgg gtccttctgc
    16321 acggccgaga catggatgcc gcctttatgc gcgaaggccg actctcccac ataaggcgcg
    16381 cgacggttcg gcgcccggtt caagacctcg tcgagaacgc gcgaggcgtg gctgagcgtc
    16441 ttgagcttgt ccgccgtcac gcccgtctcg aaccgcgccg catagtcggg cttcagcaac
    16501 agcgtgggga tgatggaggt gaggttggcg ttgccgcagc gttcgccgag cccgttcagc
    16561 gtgccttgga tctgccgggc gcctgcgcgg accgcggcga gcgaattcgc gacgccattg
    16621 tcggtgtcgt tgtggacatg gatgccgaga tggtcgccgg ggatgtgctt gacgatctcg
    16681 gtcacgatcc tttcgatctc ctcgggcagc gtgccgccat tggtgtcgca gagcacgacc
    16741 cagcgcgcgc ccgcgtcata ggccgtctgg gcgcaagcga gcgcgtaagc cgaattggcc
    16801 ttgtagccgt cgaaaaaatg ctcgcagtcg atcagcgcct cgcgcttgct cgcgacgatg
    16861 gcctcggtcg actctttgat gccggcgaga ttctcggcga ggctgatacc gagcgcgacc
    16921 tcgacgtgaa agtcccaaga cttggcgacg aggcagatcg aggagacctt ggcggcgagc
    16981 agcgcctgga agccggggtc attggcgatg gagcggcccg gccgcttggt catgccgaag
    17041 gcggtgaggt tcgcgcggcc gagcgggcgc gcttcggcga agagcctcgt gtcggtcggg
    17101 ttcgcgccgg ggtagcccgc ctcgatatag tcgatcccga gcgcatcgag ctgctcggca
    17161 atcagccatt tgtcctcgac cgagaaatcg acgccatggg tctgcgcgcc gtcgcggagc
    17221 gttgtgtcgt aaagggagag acgttcgcgg gtcatgctct accccctgcc cctgccccct
    17281 ctccccttgt gagagagggt gcgcttcgca gccgcgcgcg gctgcgaagc
//
LOCUS       DASUQP010000082         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1398327, whole genome shotgun sequence.
ACCESSION   DASUQP010000082 DASUQP010000000
VERSION     DASUQP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398327"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            568..1746
                     /locus_tag="VFY74_03765"
     CDS             568..1746
                     /locus_tag="VFY74_03765"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX5930859.1"
                     /translation="MLFRLLSLALLLLPAPALAGAKEKVAALAPSGVVLVMDEKGNEL
                     VAQNADKPFVPASVTKIVTAWLAMEVLGGDYRFQTRFYLDGDRKLYVRGGGDPYLISE
                     ELAPLATQLVAAAGKEPLTGIVLDASYYPSDLRIPGIEDTDEAYDALNSALAVNFNTI
                     AAVRKGKTVRSAEQQTPITPLAISQFRSRGPNGRGRISLTQDPTVSLKYAGELLAAFI
                     ERAGGSVKGEITTGTVPESLEPVYVHRQSRTLSQILTGMLLGSNNYIANQVFLEIGGT
                     LGGHVSLEKSLKVANGMLAKHGLAESIHLEEGSGISRGNHFTARGLAQLLHLFEPHAA
                     LLKGGDRARYKTGTFSGVRTLAGYADTSKHGRVRFVISLRSNNGSLRFQLLRAIQSGL
                     "
BASE COUNT          380 a          668 c          620 g          381 t
ORIGIN      
        1 acggccgaac tcgctgtagc ggcctccttg agcgtgcccc agaggccgct tggatctgcc
       61 gccgatacca caattccgac gagcatcgtg ctttgcaaca ccttggtcca ctcttcgggc
      121 gtgaaattct gtttgttcgc catcgtcgtt tccctcagag tgactttgca agggtgtacg
      181 tatcccaaca tgaggccgat tgctaagacg cgcttacatc ctctgccatc cgagattggt
      241 ctagaacgga ggagattcgt gcctgtgcga gcgcctcttg cgcgcgatcg cggcgaaggc
      301 gacggcaatt aacgataaaa aagcccaggt cctcgatcgg atacgggacc aaaaggatgc
      361 ttgctccggc atgtcgcgac tcctgcaact ttcgtgcgag ttggatcgct ataggagacg
      421 cgcagtatag tcccgtcgga tttgtatgcc aatgcgaccg acgcccgctt gtgccagaag
      481 ggaaattttg cgatctggcc gctttatcgc ttgagtgtca ctggcatcgg cccacaagtc
      541 gcgtacgctg cgcagactgg agtcgcgatg ttgtttcgcc tgctcagcct cgccttgctc
      601 ctgctgcccg ccccggccct ggccggcgcc aaggagaagg tcgcggccct cgcgccgtcg
      661 ggagtggtgc tcgtgatgga cgaaaagggg aacgagctgg tcgcccagaa cgccgacaag
      721 cccttcgtcc cggcctccgt caccaagatc gtgaccgcat ggctggcgat ggaggtcctg
      781 ggcggcgact accgcttcca gacgcgcttc tacctggacg gcgaccggaa gctctacgtg
      841 cgcggtgggg gcgatcccta tctgatttcc gaggaactgg cgccgctcgc gacccagctg
      901 gtcgccgcgg ccggcaagga gccgctcacc ggtatcgtgc tcgatgcgag ctactacccg
      961 tctgacctcc gcatcccggg catcgaggac accgacgagg cctacgacgc gctgaactcc
     1021 gcgctcgcgg tgaacttcaa cacgatcgcc gccgtgcgca agggcaagac ggtccgctcc
     1081 gccgagcagc agaccccgat caccccgctt gcgatcagcc agttccggtc gcggggcccc
     1141 aacggtcgcg gccgcatcag cctgacccaa gaccccacgg tgagcctgaa atacgccggc
     1201 gagttgcttg ccgcgttcat cgagcgcgcc ggcggcagcg tcaagggcga gattacgacc
     1261 ggaaccgtcc ccgagagcct ggagccagtc tatgttcacc gccagtcgcg cacactttcc
     1321 cagattctca ccgggatgct cctcggctcg aacaactaca tcgccaacca ggtcttcctg
     1381 gagatcggcg ggaccctggg cggacacgtc agcctggaga agtcgcttaa ggtcgcgaac
     1441 gggatgcttg ccaagcacgg actcgccgag tcaatccatc tcgaggaagg ctcgggcatc
     1501 agccgcggca atcacttcac ggcccgcggc cttgcccagt tgctgcacct cttcgagccc
     1561 catgccgccc tgctcaaggg cggcgaccgc gcccgctaca agaccggcac cttttccggc
     1621 gtccgcacct tggcgggcta cgccgacacc tccaagcatg ggcgggtgcg cttcgtgatc
     1681 tcgctgcgga gcaacaacgg ctccctgcgc ttccaactgc tccgggcgat ccaatctggg
     1741 ctgtagtccg cgccaccgtg gcaaatgact gggcattgcg gcttaggcgc taacgcgacg
     1801 actatgcgat gagcgccaag cgcatgcata tgctgaaggg cgaggtgggc tgaagccagg
     1861 cgcattgctc gcttacggca attggtgcag gctgagaatc ggcctctgag atagaagttg
     1921 ataaccggcg gtttactcga ccggcatcac catcaggaca gctcgaattg acgggcagga
     1981 cacacgcatg tgaggacttc catgattggc aatcgcatgc gcggcttcgc actgttgacc
     2041 gcttttgca
//
LOCUS       DASUQP010000083         5870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1126433, whole genome shotgun sequence.
ACCESSION   DASUQP010000083 DASUQP010000000
VERSION     DASUQP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126433"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="VFY74_03770"
     CDS             complement(<1..100)
                     /locus_tag="VFY74_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX5930860.1"
                     /translation="MAELWVFAYGSLMWQPGFAFSERAPAALIGAHR"
     gene            complement(107..1033)
                     /locus_tag="VFY74_03775"
     CDS             complement(107..1033)
                     /locus_tag="VFY74_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEX5930861.1"
                     /translation="MSDPLFDKIAIIGMGLIGSSIARAARAKGGVRAIAATARSPKTR
                     QRVTELGLADKVVETNAEAVKGADLVIVCVPVGACGAVAKEIGPHLEPGATVSDVGSV
                     KRSVVRDMGAHLPKNIHFVPAHPVAGTEHSGPDAGFAELFEGRWCILTPDAGSDTQAV
                     DRLAAFWRLLGAKVEIMTAEHHDIVLAITSHVPHLIAYNIVGTAADMEEVTQSEVVKF
                     SAGGFRDFTRIAASDPVMWRDVFLNNKEAVLEMLGRFSEDLAALQRMIRWDDGAALEA
                     LFTRTRSIRRGIIEAGQDTEAADFGRPHGHDD"
     gene            complement(1030..2148)
                     /gene="hisC"
                     /locus_tag="VFY74_03780"
     CDS             complement(1030..2148)
                     /gene="hisC"
                     /locus_tag="VFY74_03780"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5930862.1"
                     /translation="MAAMRGPRPRPGVLDIAPYVPGTSALPGAEPVIKLSSNETPFGP
                     SPRAIEAYHAAAGALSRYPDGSARPLREAIGAFYGLDPARIVCGAGSDELLNLLASAY
                     LGPDDEAIYSAHGFLVYKIAIMARGATPVAAPETELTADVDAILACVSPRTRLVFIAN
                     PNNPTGTYLPFDEVKRLREALPDDVLLVLDAAYAEYVRKNDYESGIELVATSPNTVMT
                     RTFSKLYGLASLRIGWAYCPPSIADALNRIRGPFNVSGPAIAAGVAALGDQPWAQAAV
                     EHNEIWRGRLTEALAGIGLVVTPSAANFLLLHFPKGNGRSAPDADKFLHERRIILRRV
                     EEYGFPHALRMTIGTAEINATVVEALSAFMDANGAARR"
     gene            2349..3557
                     /locus_tag="VFY74_03785"
     CDS             2349..3557
                     /locus_tag="VFY74_03785"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX5930863.1"
                     /translation="MSETSTAQAFADHSLRLAQIANPQSEIVHFPAHEPLPLDCGAVL
                     APFSIAYQTYGVLNAAKSNLVLVCHALTGDQHVANTHPVTGKPGWWDTMVGPGKPIDT
                     NRYFVLCPNVIGGCMGSTGPSSINPATGRVYGLDFPVITVADMVRAQARLLDHLGIPD
                     VLSVLGGSMGGMQVLEWAATYKDRVFSAVPIACSARHSSQNIAFHEVGRQAVMADPDW
                     CQGRYLDEGRNPRKGLAVARMAAHITYMSDDALHTKFGRNLQDRDKVAFGFDADFQVE
                     SYLRHQGLSFVDRFDANSYLYITRAMDYFDLAADHGDVLANAFTGTKTRFCLISFTSD
                     WLFPTEESREIVHALNAVAANVSFVELKTANGHDSFLLHEPELFDTIRGFLGAAAAKR
                     GVAGAEGWLP"
     gene            3545..4195
                     /gene="metW"
                     /locus_tag="VFY74_03790"
     CDS             3545..4195
                     /gene="metW"
                     /locus_tag="VFY74_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX5930864.1"
                     /translation="MAAMNFNVPSPAIRAADTSRVDLLLIAEMVTHGARVLDIGCGDG
                     TLLRLLAEKRGVDGRGIELSQAGVNSCVAQGLSVIHGDADTDLVYYPDLAFDFAILSQ
                     TIQATYSPHHVLEQLLRIGRRVVVSFPNFGHWRVRAQLMFGGQMPQTDNLPDRWYDTP
                     NIHLCTIKDFLGLCKDVGARVERAVALNAYGRKLGVSMPLFAQNLFGQQAVFLLSR"
     gene            complement(4181..4924)
                     /locus_tag="VFY74_03795"
     CDS             complement(4181..4924)
                     /locus_tag="VFY74_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5930865.1"
                     /translation="MHLDVVDLKEFYACPLGLVVRRLLGARLRARWGDVKSCRTFGLG
                     FPAPFLATFRDEAEPLGALMPARLGAILWPENGRSLSVLVEDTELPLIDEGADRILLV
                     HMLEWSEKPLELLREVWRVLAPQGRLLLVVPNRRGLWARVDTTPFGYGSPFSRSQLTK
                     LLKKAMFSPDHWEYALHMPPFNWRILLKWPLFFERLGFLLWPTFSGVIVVEATKQVYA
                     AIPARETAKRGRRLVPVPAGVTPSTLPRE"
     gene            5120..>5870
                     /gene="gloB"
                     /locus_tag="VFY74_03800"
     CDS             5120..>5870
                     /gene="gloB"
                     /locus_tag="VFY74_03800"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX5930866.1"
                     /translation="MSRLQIHQFPTRSDNYGVLVHDPSSGATAAIDAPDAEELLAALH
                     EKGWSLSHILTTHHHHDHTAGNTIVKRMTGCTIVGPAKEASSIPDIDVAVKEGDTVEI
                     GSAKARVIETPGHTKGHVSYHFADDDLVFVGDTLFSVGCGKLLEGDAKTMWASLKKLA
                     ALPPETSLYCGHEYTNNNCRFALTVEPENDALRARAAQASALAEEGAPALPTTIAQEL
                     ATNPFLRPSSLAIQKRLGMEGRELWEIFGEIR"
BASE COUNT         1072 a         1902 c         1927 g          969 t
ORIGIN      
        1 accggtgcgc gccgatcagg gccgccggcg cccgctcact gaaggcgaaa cccggctgcc
       61 acatcagaga gccgtaggcg aacacccaaa gctcagccat gggccttcag tcgtcgtggc
      121 cgtgggggcg gccgaaatcg gcggcctcgg tgtcttggcc cgcctcgatg atcccgcgcc
      181 gaatggagcg cgtgcgggtg aatagcgctt cgagcgccgc accatcgtcc caccggatca
      241 tgcgctgaag cgcggcgaga tcctcggaga agcggccgag catttcgagc accgcctcct
      301 tgttgttgag gaacacgtcg cgccacatga cggggtcgga ggcggcgatg cgggtgaagt
      361 cgcgaaaacc gcctgccgag aacttgacga cttccgattg cgtgacctcc tccatgtcgg
      421 ctgcggtgcc gacgatattg taagcgatga gatgcggcac gtggctcgtg atcgcgagca
      481 cgatgtcatg atgctcggcc gtcatgatct cgaccttggc gccgagcagg cgccagaacg
      541 cggcgagcct gtccacggct tgcgtgtcgc tccccgcgtc cggcgtcaag atgcaccagc
      601 ggccttcgaa cagctcggcg aagcccgcgt cgggaccgga atgctcggtg cccgccacgg
      661 ggtgcgccgg aacgaaatga atgttcttcg ggagatgcgc gcccatgtcg cgcacaaccg
      721 agcgcttgac cgatcccaca tcggagaccg tggcaccggg ctcaaggtgc gggccgattt
      781 ccttagcgac cgcgccgcag gccccgacag gaacgcagac gatgacgagg tcggcgccct
      841 taaccgcttc ggcgttggtc tcgacgactt tgtcggcgag gccgagctcg gtcacgcgtt
      901 gccgcgtttt gggcgaacgc gccgtggctg cgatcgcgcg cacgccgcct tttgcccgcg
      961 cggcgcgggc gatcgaggag ccgatcaacc ccatgccgat gatggcgatc ttgtcgaaca
     1021 gcggatcgct catcgccgcg cagcaccgtt ggcgtccatg aaagccgaca gcgcctcgac
     1081 cacggtcgcg ttgatctccg ccgtgccgat ggtcatgcgc aaagcgtgcg gaaagccata
     1141 ttcctcgaca cgacgcaaga tgatgcgccg ctcgtgcagg aacttgtcgg cgtccggcgc
     1201 cgaccggccg ttccccttcg ggaaatgcaa caagaggaaa ttcgcagcac tcggcgtgac
     1261 gacaagcccg atgccggcaa gagcctcggt gaggcgcccg cgccagatct cgttgtgctc
     1321 gacggcagct tgcgcccaag gttgatcgcc gagcgccgcg acgcctgccg caatcgccgg
     1381 ccccgagaca ttgaacgggc ccctgatccg gttcagcgca tcggcgatcg agggcgggca
     1441 ataggcccag ccgatgcgga gcgaagcaag cccatagagt ttggagaagg tgcgggtcat
     1501 caccgtgttc ggcgaagtcg cgacaagttc gatgccggac tcgtaatcgt tctttcgcac
     1561 atattcggca taggccgcgt cgagtacgag cagcacatcg tcgggcaagg cttcgcgcag
     1621 gcgcttgacc tcgtcgaacg gcagataggt accggtcggg ttgttggggt tggcgatgaa
     1681 cacgagccgc gtccgcgggc tcacacaagc gaggatggcg tcgacatcgg ccgtgagctc
     1741 cgtctcgggc gcagcgacgg gtgtcgcgcc gcgcgccatg atcgcgatct tgtaaacgag
     1801 gaagccatga gcgctgtaga tcgcctcgtc gtcaggaccg agataggcgg aggcgagcag
     1861 attgagaagc tcatcagagc ccgcgccgca gacgatgcgc gcgggatcga ggccgtagaa
     1921 ggcgccgatc gcctcccgca aaggccgggc ggaaccgtcg ggatagcgcg agagggcgcc
     1981 ggccgcggcg tgataagcct cgatcgcgcg aggcgacggc ccgaacggtg tctcgttcga
     2041 ggagagcttg atgacgggct cagcgccagg gagcgcgctc gtgcctggca cgtaaggcgc
     2101 gatgtcgaga acgcctggac gggggcgcgg gccacgcatg gcggccatgg gatcttgctc
     2161 ctcggagggg gccggcgaag ggcctctcat aggcccaatc cgcgcggcaa agcaaagaaa
     2221 agattgactt tcacggtcaa gctcctagct aacgccgaat tgtgcagtct tggttaccat
     2281 tcggcgtcat accggcgaga agcgggcatc cagggcgaag cgcggcggcg tgagttgggc
     2341 ataggacgat gagcgagact tcgacggcgc aagcttttgc cgaccattcg ctgcggctcg
     2401 cgcaaatcgc aaatccgcaa agcgagatcg tgcactttcc ggcgcacgag cctctgccgc
     2461 tcgattgcgg agccgtgctt gcgccgttct ccatcgccta tcagacctac ggcgtgctca
     2521 acgcggcgaa gtcgaacctc gtgctcgtct gccatgcgct caccggcgat cagcacgtcg
     2581 ccaataccca ccccgtcacc ggcaagccgg gctggtggga caccatggtc ggccccggca
     2641 agccgatcga caccaaccgc tatttcgtgc tgtgccccaa cgtcatcggc gggtgcatgg
     2701 gctcgaccgg cccaagctcg atcaatcccg caacgggtcg cgtctacggt ctcgactttc
     2761 ccgtcatcac cgtcgccgac atggtgcggg cgcaggcgcg gctcctcgat catctcggta
     2821 ttcccgacgt gctctcggtg ctcggcggct ccatgggcgg catgcaggtg ttggaatggg
     2881 ccgcgaccta taaggaccgc gtgttctccg cggtgccgat cgcctgttcc gcgcgccact
     2941 cctcgcagaa catcgccttc cacgaggtcg gccgccaggc ggtgatggcc gatcccgatt
     3001 ggtgccaggg tcgctatctc gacgagggca ggaacccgcg caagggtctc gccgtggcgc
     3061 gcatggccgc gcacatcacc tatatgtccg acgacgccct gcacacgaag tttgggcgca
     3121 atctccagga ccgggacaag gtcgccttcg gcttcgacgc cgattttcag gtggagagct
     3181 atctgcgcca tcagggcttg agcttcgtcg accgtttcga tgccaactcc tatctctaca
     3241 tcacccgcgc catggactat ttcgacctcg ccgccgacca cggcgacgtg cttgccaacg
     3301 cctttaccgg gaccaaaacg cggttctgcc tgatctcctt cacgagcgat tggctgtttc
     3361 cgacggaaga gagccgcgag atcgtgcacg cgctgaatgc ggtcgccgcc aatgtcagct
     3421 tcgtcgagct gaagaccgcc aacggccacg actccttcct gctgcacgag cctgagctgt
     3481 tcgacacgat tcgcggtttc ctgggcgcgg cggcggcaaa gcgcggggtt gcaggagcgg
     3541 aaggatggct gccatgaact tcaacgttcc atcgccggcc atccgcgccg ccgacacgag
     3601 ccgcgtcgat cttctgctca tcgccgagat ggtgacacac ggcgcgcgcg tgctcgatat
     3661 cggctgcggc gacgggacgc tgttgcgcct cttagcggaa aagcgaggcg tcgacggccg
     3721 cggcatcgag ctgagccagg cgggggtgaa ttcttgcgtg gcgcaaggct tgtcggtgat
     3781 ccacggcgat gccgacaccg acctcgtcta ttatcccgat ctcgccttcg acttcgccat
     3841 cctctcgcag acgatccagg cgacctattc gccgcaccac gtgctcgagc agctgctgcg
     3901 tatcggcagg cgcgtcgtgg tgtcgtttcc gaatttcggc cattggcgcg tgagggcgca
     3961 gctcatgttc ggcggccaaa tgccgcaaac cgacaatctg cccgaccgct ggtacgacac
     4021 gcccaacatc catctctgca ccatcaagga ctttctcggg ctgtgcaaag atgtcggcgc
     4081 acgcgtcgag cgcgccgtgg cgctcaacgc ttatgggcgc aagctcggcg tctccatgcc
     4141 gcttttcgcg cagaacctgt tcggccagca ggcggtgttc ctactctcgc ggtagcgtgc
     4201 ttggcgtgac gccggcggga acggggacga gccggcggcc cctcttggcc gtctcgcgcg
     4261 ccggtatcgc ggcatagacc tgctttgtcg cttcgacgac gataacgccg gagaaggtcg
     4321 gccacagcaa gaagcccagc cgctcgaaga acagcggcca tttgagcagg atgcgccaat
     4381 tgaagggcgg catgtggagc gcgtactccc agtggtcggg agagaacatg gccttcttga
     4441 ggagcttggt cagctggctc cggctgaaag gactgccata gccgaacggc gtggtgtcga
     4501 cccgcgccca tagcccgcgg cgattgggga cgacgagcaa gagacgcccc tgcggcgcca
     4561 gcacgcgcca gacctcgcgc aggagttcga gcggcttctc ggaccattcg agcatgtgca
     4621 cgaggaggat gcggtcggcg ccctcgtcga tgagcggaag ctcggtgtcc tcgacaagca
     4681 cggagaggga tcggccgttc tcgggccaga gaatggcgcc cagccgcgcc ggcatcagcg
     4741 cacccagggg ctcggcctcg tcccggaacg tcgcgagaaa cggcgcgggg aagccgaggc
     4801 cgaaggtgcg gcaggatttg acgtcgcccc atctggccct aagccgcgcg ccgagaagcc
     4861 tgcgcaccac aagcccgagc gggcaggcgt agaactcctt gagatcgacc acgtcgagat
     4921 gcatgggcca tcatagcgcg tgaaggttaa gcgccgcacc ccagcctttt cacaaggcgc
     4981 ccgcgcatgc ggcatcggag gcactcgtaa cttccccctg gtggggaggt tagagattca
     5041 tgcggcgcag cggccgttca atgaggcgca caggaatgct agtgtcgttg ggcgaacgac
     5101 agagagcggc ggaggagcga tgtctcgcct gcaaatccac caattcccga cgcgaagcga
     5161 caattacggc gtgctggtcc acgacccatc gagcggggcg accgcggcaa tcgatgcgcc
     5221 cgatgccgag gagctgcttg ccgcgctgca cgagaagggc tggagcttaa gccacattct
     5281 cactacccat catcaccacg accacaccgc cggcaacacc atcgtcaagc gcatgaccgg
     5341 ctgcacgatc gtcggcccgg cgaaagaggc ttcctcgatc cccgacatcg atgtcgcggt
     5401 caaagagggc gacacggtgg aaatcggcag cgcgaaagcg cgcgtgatcg agacgccggg
     5461 ccataccaag ggtcacgtca gctaccactt tgccgacgac gatctcgtgt tcgtcggcga
     5521 tacgctgttc tcggtcggct gcggcaagct gctcgaaggc gacgccaaga ccatgtgggc
     5581 ctcgctgaag aagctcgcgg cccttccgcc ggagacgagc ctctattgcg gccatgaata
     5641 taccaacaac aattgccgct tcgcgcttac cgtggagccg gagaacgacg cgctccgcgc
     5701 gcgtgccgcg caagcctccg cccttgccga agagggcgcg ccggcgttgc cgacgacgat
     5761 cgcgcaggag ctcgccacca atcccttcct caggccttcg agcctcgcga tccagaagcg
     5821 gctcggcatg gaggggcgcg agctctggga aatctttggc gagatcaggc
//
LOCUS       DASUQP010000084        11834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1399568, whole genome shotgun sequence.
ACCESSION   DASUQP010000084 DASUQP010000000
VERSION     DASUQP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11834
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399568"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFY74_03805"
     CDS             <1..361
                     /locus_tag="VFY74_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930867.1"
                     /translation="AYVFIIIALARVVGTSWRGGDPEAETRTSRADRVCAGLLLLGFV
                     AATLAVAGSALAASQAERAPLPSWKRVGSRVERRRGEGAFDDGASRLTPHRAPNGARA
                     LPQGESGNGHAGENIAG"
     gene            complement(655..906)
                     /locus_tag="VFY74_03810"
     CDS             complement(655..906)
                     /locus_tag="VFY74_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930868.1"
                     /translation="MAFSALALCFAGAANADSYSKAVRKACGADYHKYCGEYGIESAA
                     LRVCMDKAGHNLSKACINALVHAGEVSQTEVNRRKAASR"
     gene            complement(2006..2980)
                     /locus_tag="VFY74_03815"
     CDS             complement(2006..2980)
                     /locus_tag="VFY74_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX5930869.1"
                     /translation="MSFTLIQQATPRLHRSELAVPGSNPSLFEKAAKGGADIIFLDLE
                     DAVAPDDKEQARKNIIQGLNDLDWGAKTMMIRINGLDTHYAYRDVVDVVENCPRLDMI
                     LIPKTGVPQDVYAIDMLVTQIEQYKKREKRIGFEVLIETALGMANVEAIAQSSKRLEA
                     MSFGVADYAASTRARTVGIGGPHPDYGVLTDKDAQGNRAYDWGDPWHFALSRMMVACR
                     AYGLRPIDGPFGDFSDPDGYLAAAKRAAVLGFEGKWAIHPSQVELANKVFTPSEAEVT
                     KAKRIVEAMQQAAKEGKGAVSLDGRLIDIASIRQAEGLLNKAKQCGMA"
     gene            complement(3206..5965)
                     /locus_tag="VFY74_03820"
     CDS             complement(3206..5965)
                     /locus_tag="VFY74_03820"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX5930870.1"
                     /translation="MNKVEPSPAESAPPKAIISGISPEGAADLLFRLLSEVVRRHQPE
                     IEPILVGGADITNFTPGLMARALQAQGIWFQLLSIAEQNAAMRRRRHLERTRGRESLR
                     GTFDHVLGEAARDGVAPEEVQTLLSSLRIRPVVTAHPTESKRVTVLEKYRKIYVLLAE
                     LEAARWTERERTSLIDELRDQIELVWMTGELHLEKPTVQHEVSRGLHFFDETLFEKAP
                     EMLSLVGEALAKHYPGVRFDLPPFLQFGSWIGGDRDGNPWVRTEVTAWTLRQNALAAL
                     RHYRQKIVDLTRSLSITERALPVPPNFRIELDRALARSGEGDHIKTRNPGEPYRQFLS
                     IVLRKLDARLARLKGQDVGGAGAAYETADELILDLRIVEQALEEANSPSIAADLVRPV
                     RRAVEIFRFSTVRLDLRENSTKTTAALQALWWATAGHRDDSPPEIGSREWRAWLYRAL
                     ARPLTGPHLIPDLPAEASEVIDIFAQAATARREFDRDAIGSFILSMTRSVDDILGVYL
                     LAKEGGAFLDASGTEVCALPIVPLFETIADLRAAPDIMRETLSVPLIRRSTHWQGGVQ
                     EVMIGYSDSNKDGGFIASNWELTKAQSQLTKVGTELGVPIAFFHGRGGSVSRGGAPTG
                     RAIAAQPAGSIRGRFRVTEQGEVVSYKYANRGTALYQIELLASSVFEHALKSEREEAL
                     KPKREFDDIIEALAGASRAAYTGLVSHPGLVQYFQAASPIEEISLLNIGSRPTRRFGA
                     KSLADLRAIPWVFAWAQNRHIITGWYGVGSGLHSLAQVRGQQGEALLPRLFHESRLFR
                     LIIDAVEKTLLLVDLDIARDYASLVEDEGVRELIFSMIEQEFKLTSEMVLNVSGGTAI
                     AERFPEYRRSLATRLPAVNAVSRMQVELLRRFRGAADEADRDAYKSALLLSINTVAAG
                     LGATG"
     gene            6155..6343
                     /locus_tag="VFY74_03825"
     CDS             6155..6343
                     /locus_tag="VFY74_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930871.1"
                     /translation="MTMRYAYPARLEPSDDGSILLSFRDLPEAMTFGGDMMEALASAT
                     ELPRCGLAHSAQARRARA"
     gene            6445..6585
                     /locus_tag="VFY74_03830"
     CDS             6445..6585
                     /locus_tag="VFY74_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930872.1"
                     /translation="MSRWLKSSETEIRRMLDPEHPTKLDRLNQALRALGRRLVLGDMR
                     AA"
     gene            complement(6660..7547)
                     /gene="sucD"
                     /locus_tag="VFY74_03835"
     CDS             complement(6660..7547)
                     /gene="sucD"
                     /locus_tag="VFY74_03835"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5930873.1"
                     /translation="MSILIDETTPVVVQGMTGDKGTFHAKEMIAYGTNVVGGVTPGKG
                     GQSHLDRPLFNTVKEAVKATGATTSITFVAPAFCADAIMEAADAGIRLICSITDGIPA
                     QDMMRVKRYMMRYPKDRRPMMVGPNCAGIISPGKAMLGIMPGHIYLQGKVGVISRSGT
                     LGYEAASQMKAKGIGITTSVGIGGDPINGSSFLDHLALFEEDPDTEAVLMIGEIGGPQ
                     EAEASAWIKDNMSKPVIGFVAGLTAPKGRRMGHAGAIISAAGDSAAEKAEIMRSCGLT
                     VAPNPSEFGSTVAGVLASR"
     gene            complement(7570..8745)
                     /locus_tag="VFY74_03840"
     CDS             complement(7570..8745)
                     /locus_tag="VFY74_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HEX5930874.1"
                     /translation="MDVHEYQAKELLASFGVPVPKGAVAFSPDQAVYAATELGGWNWA
                     VKAQIHAGARGKAGGIKLCKTYHEVRDAAAAMLGKRLVTIQTGPEGKPVQRVYIEVAE
                     PFERELYLGYVLDRKAERIRVIASQRGGMEIEEIAKNEPEAILQVVVEPAVGLQAFQA
                     RELAFQLGLSIKQAQSAVSTIMNAYRAFRDCDATMLEINPLVVAKNDRVLALDAKMSF
                     DDNALFRRHNVADMHDPSQQDPREAQAAEHNLNYIGLDGEIGCIVNGAGLAMATMDMI
                     KHAGGTPANFLDVGGGASPERIATALKLVLSDSRVKAVLVNIFAGINRCDWVAKGVVQ
                     AYETVDVKVPMVVRLAGTNVEEGRRILAESGLKLITAETLAEAAEKAVAACQGRLAA"
     gene            complement(8787..9419)
                     /locus_tag="VFY74_03845"
     CDS             complement(8787..9419)
                     /locus_tag="VFY74_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX5930875.1"
                     /translation="MTKFKDTAVEPFLDALASQSATPGGGSAAAIMGAMGAALVSMVC
                     NLTIGKKKYAEVEGEMRNVLAKSEALRVKLTGMIEDDVQAFDAVMAAYGMAKDSDAEK
                     AAREKAIQAALKQATDVPLRCCHAAREVIDLAAVAADKGNLNVISDAGVAVLAAYAAL
                     RSAALNVFTNARMITDKDFAEAKLKELHALLAGAESATEKAYGVVKDKLS"
     gene            complement(9419..10276)
                     /locus_tag="VFY74_03850"
     CDS             complement(9419..10276)
                     /locus_tag="VFY74_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent
                     methylenetetrahydromethanopterin/methylenetetrahydrofolate
                     dehydrogenase"
                     /protein_id="HEX5930876.1"
                     /translation="MKKLLFLLDTDPYASVFDTVVAYDGGADRVAGYANVTPDTVGPL
                     VDGTIFTRGGKDKQNTAIFVGGGDMTKGEALFTAVKKKFFGPFRVSAMLDSNGSNTTA
                     AAGVALLEKAGKLKGNKAVVLAGTGPVGMRAAAFFGQEGADVAITSRNQARANNAAKA
                     IEQRFGVKVKPIEAVDDAARAQAVKDADIVFATGAIGVQLLKAEDWQNNPKIVMLADV
                     NAQPPLGIQGVEATDKGKERSGKTAFGALGIGGLKLKLHRGCIGRLFETSESVLDAEE
                     IYALAKEMA"
     gene            complement(10281..11225)
                     /locus_tag="VFY74_03855"
     CDS             complement(10281..11225)
                     /locus_tag="VFY74_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5930877.1"
                     /translation="MQHAIVFLDRESVDAKVRKPNFPHSYQEYQSTWSAGDVVDRLKD
                     ATIAIINKVPMRADTLKQLPKLKLIAVAATGTDVVDKAYCKQNGIAVVNIRNYAFNTV
                     PEHVLALMFALRRNILAYVEDVRNGRWNKSQQFCFFDHPIRDIAGSTMGVVGYGAIGK
                     EVGRRAAALGMRVLPYDVVPQPGLVDFETLLKESDVITLHMPLTPQTKNMFGAKELKM
                     MKPTAILINTARGGLVDEAALAEALQDGTIAGAGFDVLTVEPPKDGNILLDLKLPNLI
                     VTPHVAWASQEAMQILADQLIDNVEAFVAGKPQNLVSE"
     gene            complement(11248..>11834)
                     /locus_tag="VFY74_03860"
     CDS             complement(11248..>11834)
                     /locus_tag="VFY74_03860"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX5930878.1"
                     /translation="HVENLRKYEVPVVVSVNRFSSDTDAEIALLKRLCSTMGVECVMA
                     DHWAQGGAGAEDLAHTVVRIIAEKPSDFRPLYPDQMSLLDKTRTIAQEIYGAEDITAD
                     KSVTDRFAELEKEGFGRFPICIAKTQYSFTTNPDAKGVPLGHVIPIREVRLSAGAEFV
                     VVICGDIMTMPGLPKVPAANNIELAADGRIAGLF"
BASE COUNT         1979 a         3852 c         3807 g         2196 t
ORIGIN      
        1 tgcctacgtg ttcatcatca ttgcgctcgc ccgcgtggtt ggaacctcgt ggcgcggcgg
       61 cgatcctgag gccgagacga gaacctcgcg cgccgaccgc gtttgcgccg gcctgctcct
      121 gttgggcttc gttgcggcca ccctggctgt ggccgggtcg gctctcgcgg cgtcccaggc
      181 tgagcgagcc cctctcccct cgtggaagag ggtggggagc cgcgttgagc ggcggcgcgg
      241 tgagggggct ttcgatgacg gcgcttcgcg ccttacccct caccgcgcgc cgaacggcgc
      301 gcgtgctctc ccacaagggg agagcggaaa cggacacgct ggagagaata ttgccggatg
      361 atcgcgacgc ggcggcgcct aaccggcggc ggcgtggacc gcgagccaga ccgggcgcaa
      421 gcaggtgtca ggcgtcaggt gtcaggtgtc aggctcgccc caacatctga cacccgaaac
      481 ctgacacctg acacctggtg agacgatccg ctcgatgcgc aagccccgac gccggtagag
      541 cgtctccgca agctccccgg caagtggctg cgcgggaagg ccgcggctga ggcttccggc
      601 cttcacctcc accgcctagg gcacctcgat gcgctcggtg gctagcgctc gcggctagcg
      661 actcgcggcc ttgcggcgat tgacttcggt ctggctgacc tcgcccgcgt ggaccagggc
      721 attgatgcaa gccttggaca ggttgtgccc cgccttgtcc atgcacacgc gcagcgccgc
      781 gctctcgatc ccgtactcgc cgcaatattt gtgatagtcc gcgccgcacg ccttccgaac
      841 ggccttgcta taggaatctg cattcgccgc cccggcgaag caaagcgcca gcgccgagaa
      901 cgccaagacg caagcgccaa gtgacctcgc aggtccaatc cgcatggaag cctcctcccc
      961 ggtttgattc acgggatgaa gcttaagctg aaatgcccgc gttcgttccg gctccacgcg
     1021 gcgtcagccc aaaaaactgt gcatattgtc tttcggccac agccgaagct tcacccggtc
     1081 tgcgagcgag cgccgcgcgc gctcgaacag accaaggaac agcacccgca tcggcgtgca
     1141 attcggcggg gatcccttcc gccgccgccc aagacggaaa gcagcgagtc gatagccact
     1201 cgctattggg aaaacgcgcg cggtccgtcc accttacgct tgttcgaacg gcactcctgg
     1261 ccaagcctct cggacgtcgg atcaaccggc cagatcttta aggagaaccc tgatgaagaa
     1321 gctcgttatc gccgttacgc tcgtgatcgc ctccgccagc cccgttctgg ccgacgcttt
     1381 cccgaacgtc tacatgaccc gctaattcgc tgccgtgagg cagtggtcga ggttccaatg
     1441 acaaacctct tcccacttcc ctgttgggca cccgccctca agcgggtgcc catccccctt
     1501 caagtcctcg acgaggcttt cattcgcgga attcaatttg ccgcgagcgc gctcgacaag
     1561 gcccgcgcct tggaccccgc gttcagcttt gctccctttg ccgctcccct ctcccaaaaa
     1621 ggagaggacg ccggccggtt cgcacccggc cagcaggggt gaggggttta accttcgcct
     1681 agcccctcgg ccggcacgct gtttatttgc gctaccgcta cgctgcttta gcgcaaggtc
     1741 cgtgccggcc tctccccttt gaaagaaata acatcgccac tgtttcagtc gtcatcgccg
     1801 ggcttgaccc gcctacgcgg gccgaagcct cgtggccact cccgccagcc atcgcttcgg
     1861 cgggcgaacg cccggcgatc cattccattg cctctcaacg cgctgaaccg taacggcatg
     1921 gatgcccgga caagctcgcg cccgggccgg cgaatccgga cccgggagtc cggacatgac
     1981 gccataagga tgcaggcgga gcgacttaag ccatgccgca ctgcttggcc ttgttgagga
     2041 gcccctcggc ctggcggatc gaggcaatat cgatcagccg gccgtcgagc gacacggcgc
     2101 ccttcccttc cttcgccgcc tgctgcatgg cctcgacgat gcgcttggcc ttggtgactt
     2161 ccgcctctga cggcgtgaac accttgttgg caagctcgac ctggctcggg tggatcgccc
     2221 atttgccctc gaagccgagc acggcggcgc gctttgccgc ggcgagatag ccatcgggat
     2281 cggagaagtc gccgaacggc ccgtcgatgg ggcgaagccc gtaggcgcgg caggcgacca
     2341 tcatgcgcga cagcgcgaaa tgccagggat cgccccaatc ataggcgcga ttgccttgag
     2401 cgtctttgtc ggtcagcacg ccatagtcgg gatgcggacc gccaatgccg acggtgcgcg
     2461 cgcgggtcga ggccgcatag tcggcgacgc cgaagctcat ggcctcgagc cgcttggagc
     2521 tttgggcgat cgcctcgaca ttggccatgc cgagcgcggt ctcgatcaga acctcgaagc
     2581 cgatccgctt ctcgcgcttc ttgtattgct cgatctgggt caccagcatg tcgatggcgt
     2641 aaacgtcctg gggaacgccg gtcttgggga tcaggatcat gtcgagacgc gggcaattct
     2701 caaccacatc gaccacgtcg cggtaggcgt agtgggtgtc gagaccgttg atgcggatca
     2761 tcatggtctt cgcgccccaa tcgagatcgt tgaggccctg gatgatgttc ttgcgcgcct
     2821 gctccttgtc gtcaggcgcg acggcatcct cgagatcgag gaagataatg tcggcgccgc
     2881 ctttggccgc cttctcgaac agcgaggggt tggaccccgg cacggcaagc tctgaacggt
     2941 gaagacgcgg agtcgcttgt tggatcaaag taaaactcat gtcactgctc tccctctctg
     3001 gatttggact gaagtggtgc gactattcgg acgagccccg cacaccctac ctccccctcg
     3061 tggggaggtc ggcttgcgta gcaagccggg tgggggatcg cgcggcgccg ctcaccccac
     3121 ccggctcggc ctgcggccga gccaccctcc ccatcaaggg gagggacgaa atctctctgt
     3181 cctcttctat acatcattct atttcttagc ctgtggcgcc aagaccggcg gcaacggtgt
     3241 tgatggataa aagcaaggcg gacttgtaag cgtcgcgatc ggcttcgtcc gcggcgccgc
     3301 ggaagcggcg caagagctcg acttgcatgc ggctcacggc attgacggca ggaaggcgag
     3361 tggcgagcga acggcggtac tcggggaagc gctcggcgat cgccgttccg ccgcttacgt
     3421 tcagcaccat ctcgctcgtc agcttgaact cctgctcgat catgctgaag atcagctcgc
     3481 gaacgccttc atcctcgacg aggctcgcat agtcgcgggc gatatcgaga tcgacgagga
     3541 gaagcgtctt ctccaccgcg tcgatgatga gacggaacag gcgcgactcg tggaagagcc
     3601 ggggcagcag cgcctcgccc tgctggccgc gtacctgggc gaggctatgc aggcccgagc
     3661 ccacgccgta ccagccggtg atgatgtggc ggttctgcgc ccaggcaaac acccagggaa
     3721 tggcacgaag gtcggcgagc gacttggcgc cgaaacggcg cgtcgggcgg gaaccgatat
     3781 taagcagcga gatttcctcg atcgggctcg ccgcctggaa gtattggacg aggcctggat
     3841 gcgatacgag gccggtatag gcggcacgcg aggcgcccgc gagcgcctcg atgatgtcgt
     3901 cgaactcacg cttcggtttc aacgcctcct cgcgctcgga tttgagcgca tgctcgaata
     3961 cgctggaggc aagcagctcg atctggtaaa gcgcggtgcc tctgttcgca tatttatagg
     4021 atacgacctc gccctgctcg gtgacgcgga agcgcccgcg gatcgagccg gcgggctggg
     4081 cggcgatcgc gcgccctgtc ggggcgccgc cgcgggagac cgagccgccg cggccgtgga
     4141 agaaggcgat tggaacgccg agctcggtgc cgactttggt gagctgagac tgggccttgg
     4201 tgagctccca gttcgaggcg atgaaaccgc catccttgtt cgaatcggaa tagccgatca
     4261 tcacttcctg cacgccgccc tgccaatggg tgctgcggcg gatcagcggc accgagagcg
     4321 tctcgcgcat gatgtcgggc gcggcgcgga ggtcggcgat ggtctcgaac agcggcacga
     4381 tgggaagcgc gcagacttcc gtcccggacg catccaaaaa cgcgccgcct tccttggcaa
     4441 ggagatagac gccgaggata tcgtcgaccg agcgggtcat cgacagaatg aacgagccga
     4501 tcgcgtcgcg atcgaactcg cgccgcgccg ttgcggcttg agcgaagata tcgatcacct
     4561 ctgacgcctc ggccgggaga tcggggatca ggtggggtcc ggtcagaggc cgcgccaatg
     4621 cgcggtaaag ccacgcgcgc cattcgcggc tgccgatttc aggcgggctg tcgtcgcggt
     4681 gacccgcagt cgcccaccag agcgcctgga gcgccgcggt cgtcttggtc gagttctcgc
     4741 gcagatccag gcgcacggtg gaaaagcgga agatctccac ggcgcgcctg accggcctca
     4801 ccagatcggc ggcgatcgaa gggctgttgg cctcttcgag cgcctgctcg acgatacgaa
     4861 ggtcgaggat cagctcgtcg gcggtctcgt aggcggcgcc ggcaccgccg acgtcctgtc
     4921 ccttgagccg ggcaagcctt gcgtcgagct tccgcagcac gatcgacagg aactgacggt
     4981 acggctcacc ggggttgcgg gtcttgatgt gatccccttc gccgctgcgg gcgagcgcgc
     5041 gatcgagctc gatgcggaag tttggcggca cgggaagggc gcgctcggtg atggagagcg
     5101 accgcgtcag atccacgatc ttctggcgat agtggcgcag cgcggcgagc gcgttctgac
     5161 gcaatgtcca ggccgtgacc tcggtgcgaa cccagggatt gccgtcgcgg tcgccgccga
     5221 tccacgatcc gaattggagg aagggcggca gatcgaaccg gactccggga tagtgcttgg
     5281 cgagcgcctc accaacgagg gagagcatct ccggcgcctt ctcgaacagg gtttcgtcga
     5341 agaagtgaag cccgcggctg acttcgtgct gcacggtcgg cttctccaga tgcagctctc
     5401 ccgtcatcca cacgagttcg atctggtcgc ggagctcgtc gatcagcgag gtccgctcac
     5461 gctcggtcca gcgcgcggct tccagctcgg ccaacagcac atagatcttg cggtacttct
     5521 ccagcacggt gacgcgcttc gactcggtcg gatgggcggt cacgaccggc ctgatgcgca
     5581 agctcgacag aagcgtctgg acttcctccg gcgcgacacc gtcgcgcgca gcctccccga
     5641 gcacgtggtc gaaggtgccg cgcagcgatt cgcgcccgcg cgtccgttcg aggtggcggc
     5701 gccggcgcat ggcggcgttc tgctcggcga tggacagaag ctggaaccag atgccttgcg
     5761 cctgaagcgc ccgcgccatc agaccggggg tgaagttggt aatgtcggcg ccgccgacga
     5821 ggataggttc gatctcgggc tggtgccggc gcacgacctc ggagaggagc cgaaacagga
     5881 gatcggccgc cccctccggc gagatgccgc tgatgatggc cttgggcggc gcggactcgg
     5941 cgggtgacgg ttccacctta ttcatgacgc ggcacagcac tccgaagcgc aacgtttgga
     6001 tataggttgg cgcgccgcaa acgcgcccct cctcatccgg ctctccttag aacatatctt
     6061 tccgggaagc gccgcacagt ttttggagtc atttggcaat cgcacatttt tgtacgattc
     6121 attaacctga aaagaaccaa gttgaagttg gagaatgacg atgcgctacg cctaccctgc
     6181 ccggcttgag ccgagcgatg acgggtcgat cctcctctcc ttccgcgacc tgcctgaggc
     6241 catgaccttc ggtggggata tgatggaagc gctcgccagc gcgacggaac tgcctcgatg
     6301 cggccttgct cattcggctc aagcgaggag agcccgtgcc tgagccgagc cgcacggagc
     6361 cgcacgagaa gatcgtcccg gcgagcctcg gcgttgcggc caaggcggca ttcgctcgtg
     6421 ctttcgctga gagcggcatg acacgtgtcg cgctggctga agagctccga gacggaaatt
     6481 cggcgcatgc tcgatccgga acaccccacc aagcttgacc ggctcaacca agccttgcgg
     6541 gcgcttgggc gccggctcgt gctaggcgac atgcgcgcgg cgtgacatgc gcccggaagt
     6601 cgtgcagagc agcctagaac tggttggctc agcggcgtca acacactcgc acggcgctat
     6661 caccgggagg caaggacccc tgcgaccgtc gagccgaatt cgctcggatt gggcgcaacc
     6721 gtgagcccgc aggagcgcat gatctcggcc ttttcggcgg cgctgtcgcc ggcagccgag
     6781 atgatggcgc cggcatggcc catgcggcgt cccttcggcg cggtcaggcc ggcgacaaac
     6841 ccgatcaccg gcttcgacat gttgtccttg atccaggccg aggcctcggc ctcctgaggg
     6901 ccgccgatct cgccgatcat cagcacagcc tcggtgtccg gatcctcctc gaacagagcg
     6961 aggtgatcga ggaaggacga gccgttgatg ggatcgccgc cgataccgac cgaggtcgtg
     7021 atgccgatgc ccttcgcctt catctgcgag gcagcctcgt agccgagcgt gcccgagcgc
     7081 gagatcacgc ccaccttgcc ttgcagatag atatgacccg gcatgatgcc gagcatggcc
     7141 ttgccggggc tgatgattcc ggcgcagttc ggcccgacca tcatcggccg gcggtccttg
     7201 ggatagcgca tcatgtagcg cttcacccgc atcatgtcct gcgcaggaat gccgtcggtg
     7261 atggagcaga tcagccggat gccggcatcg gccgcctcca tgatggcgtc ggcgcagaag
     7321 gcaggcgcca cgaaggtgat ggaggtggtg gcaccggtcg ccttcaccgc ctctttcacc
     7381 gtgttgaaca gagggcgatc gagatggctc tggccgcctt tgccgggcgt cacgccgccg
     7441 accacattgg tgccgtaggc gatcatctcc ttcgcgtgga aggtgccctt gtcgccggtc
     7501 atgccttgca cgacgaccgg cgtggtttca tcgatcagaa tgctcatatt tctctccgat
     7561 cctctcgcgc taagccgcaa gccggccctg acacgctgcc acggccttct ccgccgcttc
     7621 ggccaaggtc tcggcggtga tgagcttgag cccgctctcc gccagaatcc tccgcccctc
     7681 ttccacgttg gtgccggcca gccgcacgac catcggcacc ttgacgtcga cggtctcgta
     7741 ggcctgcacc acgcccttgg cgacccaatc gcagcggttg atgccggcga agatgttgac
     7801 gagcacggcc ttgaccctgg aatccgacaa gacgagcttg agcgcggttg cgatgcgctc
     7861 cggcgaggcg ccgccgccca cgtcgaggaa gtttgcgggt gtgccgccgg catgcttgat
     7921 catgtccatc gtcgccatgg cgagccccgc gccattgacg atgcagccga tctcgccgtc
     7981 gagcccgata taattgagat tgtgctcggc ggcttgcgcc tcgcgcgggt cctgctgcga
     8041 gggatcgtgc atgtcggcca cgttgtggcg gcggaacagc gcgttgtcgt cgaacgacat
     8101 cttggcgtcg agcgcgagca cgcggtcgtt cttggcgacg acgagcggat tgatctcgag
     8161 catggtcgcg tcgcaatcgc ggaaggcgcg ataggcgttc atgatcgtcg agaccgcgct
     8221 ctgcgcctgc ttgatgctga gcccgagctg gaaggcaagt tcgcgcgcct gaaaggcctg
     8281 gaggcctacg gccggctcga ccaccacttg gaggatggcc tctggctcgt tcttggcgat
     8341 ctcctcgatc tccatgccgc cgcgttgcga ggcgatgacg cggatgcgct cggccttccg
     8401 gtcgagcacg tagccgagat agagctcgcg ttcgaacggc tccgccacct cgatatagac
     8461 ccgctgcacg ggcttgcctt cgggcccggt ctggatggtg acgagccgct tgccgagcat
     8521 ggcggcggca gcgtcacgca cctcgtgata ggtcttgcac aatttgatgc cgccggcctt
     8581 gccgcgcgcg ccggcgtgaa tctgagcctt caccgcccag ttccaaccgc caagctcggt
     8641 cgcggcgtag accgcctgat ctgggctgaa ggcaaccgca cccttgggca ccggcacacc
     8701 gaaactcgcc agcaattcct tggcttgata ctcgtgaacg tccacgtctt tcctccctta
     8761 tccggtgctg ggggctagac ccaacctcag ctcagtttgt ccttgaccac gccgtaggcc
     8821 ttctcggtcg cgctctcggc tcccgccaac agcgcatgaa gttccttgag cttggcctcg
     8881 gcgaaatcct tatccgttat cattcttgca ttggtgaaaa cattcaaggc ggcgcttcgc
     8941 aatgcagcat aagcggcgag gacggcaacg cccgcatcgg aaatcacgtt gagattgccc
     9001 ttgtccgcgg cgacggcggc aaggtcgatc acttcgcgcg ccgcgtggca gcagcgcaag
     9061 gggacgtcgg tcgcctgctt cagcgcggcc tgaatggcct tctcgcgcgc ggccttttcg
     9121 gcgtcgctgt ccttggccat gccgtaggcc gccatcaccg cgtcgaaggc ctgcacgtcg
     9181 tcctcgatca tgccggtgag tttcacccgg agcgcctcgg acttggcgag cacgtttctc
     9241 atctcgccct cgacctcggc gtacttcttc ttgccgatgg tcaggttgca caccatgctg
     9301 acgagggccg cgcccatggc gcccatgatg gcggcggcgc tgccgcctcc gggcgtcgca
     9361 ctctggctcg ccagagcgtc gagaaatggc tcgaccgccg tgtccttgaa cttcgtcatc
     9421 aggccatctc tttcgccaag gcgtaaatct cctcggcatc gagcacgctt tctgaggttt
     9481 cgaacagccg gccgatgcag ccgcgatgca gcttcaattt gagtccgccg atgccgagcg
     9541 cgccgaaggc ggtcttgccg ctgcgctcct tgcccttgtc ggtggcttcg acgccctgga
     9601 tgccgagcgg gggctgcgcg ttgacgtcgg cgagcatcac gatcttgggg ttattctgcc
     9661 agtcttcggc cttcaagagc tgcacgccga tggcgccggt ggcgaagacg atgtcggcat
     9721 ccttcaccgc ctgggcgcgg gcggcatcgt ccacggcctc gatcggcttg accttcacgc
     9781 cgaaccgctg ctcgatcgcc ttggcggcgt tgttggcgcg cgcttggttg cgcgaggtga
     9841 tggcgacgtc ggcgccctcc tggccgaaga aggcggccgc gcgcatgccg acggggccgg
     9901 tgcccgccag caccacggcc ttgttgcctt tcagcttgcc cgccttctcg agcagcgcca
     9961 cgcccgccgc agcggtggtg ttggagccgt tcgaatcgag catggccgag acgcggaagg
    10021 gtccgaagaa ttttttcttc accgccgtga acagcgcctc gcccttggtc atgtcgccgc
    10081 cgccgacgaa gatcgccgtg ttctgcttgt ccttgccgcc gcgggtgaag atcgtgccgt
    10141 cgaccagggg accgacagtg tcgggcgtga cattggcgta gcccgcgacc cggtcggcgc
    10201 cgccgtcata ggcgaccacc gtatcgaaga cgctggcata agggtcggtg tcgagcagga
    10261 acaggagctt cttcatcggc tcactccgac acgagattct gcggcttgcc tgccacgaac
    10321 gcttcgacgt tgtcgataag ctgatcggcc aggatctgca tcgcctcctg gctcgcccag
    10381 gcgacgtggg gggtgacgat caggttgggc agcttgaggt cgagcaggat attgccgtcc
    10441 ttgggcggct caacggtcaa gacatcgaag cccgcgccgg cgatggtgcc gtcctgaagc
    10501 gcctcggcga gcgccgcctc gtccaccagc ccgccgcgcg cggtattgat gaggatggcg
    10561 gtcggcttca tcatcttgag ctccttggcg ccgaacatgt tcttcgtttg cggcgtgagc
    10621 ggcatatgca aggtgatgac gtcgctctcc ttcaggagcg tctcgaaatc gacgaggccc
    10681 ggctgcggca ccacatcgta gggaagcact cgcatgccga gcgccgcggc ccttctgccg
    10741 acctccttgc cgatggcgcc ataaccgacg acccccatgg tggagccggc gatgtcgcgg
    10801 atcgggtggt cgaagaaaca gaattgctgc gacttgttcc atctgccgtt gcgcacatcc
    10861 tcgacatagg cgaggatgtt ccgccgaagc gcgaacatca gcgcgagcac atgctcgggc
    10921 acggtgttga aggcgtagtt gcggatattg acgacggcga tgccgttttg cttgcaatag
    10981 gccttgtcca ccacgtcggt gccggtggcc gcgaccgcga tcagcttcag cttcgggagc
    11041 tgcttcagcg tgtcggcgcg catcggcacc ttgttgatga tggcgatggt cgcgtccttc
    11101 agccggtcga ccacgtcccc cgccgaccag gtcgactgat attcctggta gctgtgcggg
    11161 aaattcggct tgcggacctt ggcgtcgacc gactctcgat cgagaaacac gatcgcgtgc
    11221 tgcactgaac tcaactcctc tcaactccta aaacaggccg gcgatccgtc cgtcggccgc
    11281 aagctcgata ttgttggctg ccggcacctt gggcaggccc ggcatggtca tgatgtcgcc
    11341 gcagatcacc acgacgaact ccgcccccgc cgagagcctg acctcgcgga tcgggatgac
    11401 gtgcccgagc ggtacgccct tggcatcggg attggtggtg aagctgtatt gggttttggc
    11461 gatgcagatc gggaacctgc cgaaaccttc cttttcgagc tcggcaaagc ggtccgtcac
    11521 gctcttgtcg gcggtgatgt cttcggctcc gtagatctcc tgggcgatgg tgcgggtctt
    11581 gtccaagagc gacatctggt cgggatagag cggccggaag tcgctcggtt tctcggcgat
    11641 gatgcggacc acggtgtggg cgagatcttc ggcacccgcc cctccttgcg cccagtgatc
    11701 ggccatcacg cattccaccc ccatcgtgga gcagaggcgc ttgagcagcg cgatctcggc
    11761 atcggtatcg ctggagaagc ggttcaccga caccaccacc ggcacctcgt acttgcggag
    11821 attctcgacg tggc
//
LOCUS       DASUQP010000085         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1537282, whole genome shotgun sequence.
ACCESSION   DASUQP010000085 DASUQP010000000
VERSION     DASUQP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537282"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..770
                     /locus_tag="VFY74_03865"
     CDS             480..770
                     /locus_tag="VFY74_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930879.1"
                     /translation="MRDILADLRDRARFLEQEIHAENARFDQQVSGLTAERDGAIQHM
                     RAQLLLANKLIEFTAWHHKVRTVLAAHIAVAEIAENSIARSLQAGAELSRSS"
     gene            complement(865..2127)
                     /locus_tag="VFY74_03870"
     CDS             complement(865..2127)
                     /locus_tag="VFY74_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5930880.1"
                     /translation="MRALTRRQFGAGAGAALAAVAAPRLARAAAKPPVAVIGGGVGGA
                     TAARYLAREGLDVTLIEAKERYVTCFFSDLYLAGLRSLDSLTHGYDRLAQNGGIKIVH
                     ATATGIDPVSKIVSLNNGTKLSYERLVVSPGIAFKFGAIEGYDEAATAVMPHAWMAGP
                     QTELLRKQLEAMEDGGTFVIAIPPDPFRCPPGPYERASLAAHYFKQNKPHSKIIVLDA
                     KDAFKAQELFEEAWARRYPGMIEWLPMQFTGGVKAVDVKGGVVVAANARFPAAVANII
                     PAQMAGQPAEAAGLTDKSGWCPVDPVTFASKLQPDIHVIGDAAIGGDMPKSAFSANTQ
                     AKTCAFAIAAALNGTPPAAAHLFNSCYTFLGPDDAFTNAINFAPVDGKLKMTDFFISK
                     VNATTQERRRMAAEAGGWYQAFMRDVFG"
     gene            complement(2124..2477)
                     /locus_tag="VFY74_03875"
     CDS             complement(2124..2477)
                     /locus_tag="VFY74_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5930881.1"
                     /translation="MHTAFGRRGLVQISLAAVLAIGAAIAVSGALEGGAAEFDQGARL
                     ASICAACHDSDGKSDGIPPLTGLDENEITEALARYRASESPSIVMHAIALSLTAEEVR
                     SVARYLADKGKEARP"
     gene            complement(2507..2833)
                     /gene="soxZ"
                     /locus_tag="VFY74_03880"
     CDS             complement(2507..2833)
                     /gene="soxZ"
                     /locus_tag="VFY74_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX5930882.1"
                     /translation="MTTPVPRIVVPESVRKGEAFTVKTIISHPMETGLRKDEHGSVIP
                     RKIINRFACTYGGVLVFSVDLHEAVSANPFFEFELVATESGRLQFVWEEDGGALYGLS
                     HDLVVE"
     gene            complement(2857..3309)
                     /locus_tag="VFY74_03885"
     CDS             complement(2857..3309)
                     /locus_tag="VFY74_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX5930883.1"
                     /translation="MELTRRELLRSVCAGTAALAGAALPPVSSIAGDDALAFVEKTFD
                     RGATMSSRVQLTMPSVFPTGYTVPMDIAIDTAMTDNDRVRSVRVFAPGNPLLEVAGFN
                     FVAGRSVPRVSTRIRLAKPQFVVAIAEMNDGALLANKVWVDVATDGCA"
BASE COUNT          652 a         1126 c         1144 g          640 t
ORIGIN      
        1 tgtcttgaag tgtggtggca tgcctggcgc gggtcaacgt gctgcttccg cctgcctccc
       61 atccggcgcg gttaccccgg gcagaaggcc tcattcacca cgtcctcgtc atggccgggc
      121 gcagtcccgg ccatccacga atttcagcgc aacgaatcga aacgtatcct cagccaacat
      181 accaacgccg tcatgctgag gagcgcccgt gaggggcgcg tctcgaagca cgtctttagt
      241 atgcgccttt cgagatcggc ctccggccct cctgagagtg agaggagtca gggcctgaat
      301 ggccgggcca taggttcacc ttgccccggc ttgccaagga ggcgactgtg cataaccggg
      361 aaaacgagaa ttgagtcctt gacgaacccc ccgccgcttt cttggctgac aagattggac
      421 ggacactgtc gttacggacc cgcggggggc atcgggggcg ggagcagggg gcatcgctca
      481 tgcgtgacat tcttgccgac ttgcgcgacc gcgcccgttt cctcgagcag gaaatccatg
      541 cggagaacgc gcgttttgac caacaggtgt cggggctcac ggcagaacga gacggcgcga
      601 tccagcatat gagggctcag ctacttctgg cgaacaagct gatcgagttc acggcttggc
      661 accacaaggt ccgaaccgtc ctcgcagcgc atattgctgt ggctgaaatc gctgaaaact
      721 ccattgcaag gtcgcttcag gcaggcgccg agctttcccg ctcttcgtaa atccgtccgt
      781 gccaaagccc tcatgctgag gagcgcgcgt aagcgcgcat tctcgcgtgc ggcctaacga
      841 gcgcccgagg cgcgggtccg ccgatcagcc gaagacgtcg cgcatgaagg cttggtacca
      901 gcctcccgcc tcggccgcca tgcggcggcg ctcctgcgtg gtggcattga ccttgctgat
      961 gaagaaatcg gtcatcttca gcttgccgtc gaccggcgcg aaattgatcg cattggtgaa
     1021 ggcgtcgtca ggaccgagaa atgtgtagca ggaattgaac agatgcgccg ccgcgggcgg
     1081 cgttccgttc agcgcggcgg cgatggcgaa ggcgcacgtc ttggcctggg tgttggcgga
     1141 gaaggcggat ttcggcatgt cgccgccgat agcggcgtcg ccaatgacgt gaatgtcggg
     1201 ctgaagcttc gaggcgaagg tcacaggatc gaccgggcac cagcccgatt tgtccgtgag
     1261 gccagccgct tccgccggtt gccccgccat ctgcgccgga atgatattgg cgaccgcggc
     1321 cgggaagcgc gcattcgccg ccaccacgac tccgcctttc acgtcgaccg ccttgacgcc
     1381 gcctgtgaac tgcatcggca gccactcgat catgccggga tagcgccgcg cccacgcctc
     1441 ctcgaacagc tcctgcgcct tgaaggcatc cttggcgtcg agaacgataa tcttcgagtg
     1501 gggtttgttc tgtttgaagt aatgagcggc gagcgaagcg cgctcgtaag gcccgggcgg
     1561 gcagcggaac ggatcgggcg gaatggcgat gacgaaagtg ccgccgtcct ccatcgcctc
     1621 gagctgtttc ctgagcagct cggtctgcgg ccccgccatc cacgcatggg gcatgaccgc
     1681 cgtggccgct tcgtcgtaac cttcgatcgc gccgaatttg aaggcgatac ccggcgacac
     1741 cacaagccgc tcataagaga gcttggtgcc gttattgagt gaaacgatct tgctcaccgg
     1801 atcgatgccg gtcgccgtgg cgtggacaat tttgatcccg ccgttttgcg caagcctgtc
     1861 atagccgtgg gtcagcgagt cgagcgagcg caatccggcg agatagagat cgctgaagaa
     1921 acacgtgacg tagcgctcct tggcttcgat gagggtgacg tcgaggcctt cgcgcgcgag
     1981 atagcgggct gcggtggcgc cgccgacgcc gccgccgatc accgcgaccg gcggttttgc
     2041 cgcggctcgt gccaggcggg gcgcggccac ggcggcgagc gcggcaccgg cgccggcacc
     2101 gaattggcgg cgggtcaagg ctctcacggc ctcgcctcct tccccttatc ggcgagatag
     2161 cgcgccacgc ttctaacctc ctccgcggtg agcgagagtg cgatcgcatg catgacgatg
     2221 cttggggatt cgctggcacg atagcgcgcc agcgcctcgg tgatctcatt ctcgtcgaga
     2281 ccggtgagcg gcgggatgcc gtcgcttttt ccatccgaat cgtggcaggc ggcgcagatt
     2341 gaagcgagcc gcgcgccttg atcgaactcg gcagcaccgc cttctagggc acccgacacg
     2401 gcaattgcgg cgcctatcgc aaggaccgcg gccagcgaaa tttgaacaag accgcgccgg
     2461 ccgaacgcgg tatgcattgt tttacgcatc ccttgccggc gcaaagctat tcgaccacaa
     2521 gatcgtgcga gagcccgtaa agcgcgccgc catcctcttc ccaaacgaat tgcaacctgc
     2581 cgctctccgt ggccacaagc tcgaactcga aaaagggatt ggccgagacc gcctcgtgca
     2641 gatcgacgct gaacacgagc accccgccgt aagtgcaggc aaacctattg atgatcttgc
     2701 gcggaatgac gcttccgtgc tcgtccttgc gaagtcccgt ctccatcgga tggctgatga
     2761 tggtcttgac cgtgaaggct tcgcctttgc gcaccgactc cggtacgacg atgcgcggaa
     2821 cgggtgtggt cacgacttca agcctcctgc cagcggtcaa gcgcagccgt cggtggcgac
     2881 gtcaacccaa accttgttcg cgagcagcgc gccgtcgttc atctcggcga tggcgacgac
     2941 gaattgcggt ttggcgagcc tgatgcgcgt cgacacccgc ggtacactcc gccccgccac
     3001 gaaattgaaa ccggcgactt caaggagcgg gtttcccgga gcgaacacgc gcacgctcct
     3061 gacacgatcg ttgtcggtca tcgcggtgtc gatcgcgata tccatgggga cggtgtagcc
     3121 ggtcgggaat accgacggca tcgtgagctg cacgcggctc gacatcgtgg cgccgcggtc
     3181 gaaagttttt tcgacgaacg cgagcgcgtc gtcgccggcg attgaagaaa caggtggaag
     3241 cgccgcaccg gcgagtgcgg ctgtcccggc gcaaacgctc ctcaacagtt cgcggcgcgt
     3301 caattccatg cgtcaactcc acgacgccgg gctcgaaggc cccaggctcg cccccgccct
     3361 cttagtgtag caaagagcgc ccgaacgtaa atggccgtaa cggttactgc cacggcctcg
     3421 acagcggcgg tcgtggagcg tgactgggtc agaaatccac gtcgagatcg ccgtgatgat
     3481 ggtgccctgg gagttcgggc gggggcggcc ccggcacttc ggccaccatg gccccggtgg
     3541 tgatgaggag gccggccacc ga
//
LOCUS       DASUQP010000086         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1946916, whole genome shotgun sequence.
ACCESSION   DASUQP010000086 DASUQP010000000
VERSION     DASUQP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946916"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFY74_03890"
     CDS             complement(<1..1150)
                     /locus_tag="VFY74_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5930884.1"
                     /translation="MSEYRHVYESWKADPEAFWADAADAVDWIKPYDKVFDEGAGQYG
                     RWFPGALCNTAYNCLDRHVDSGQGAATALIYDSPVTNTKRKFTFSELSDEVATFAAVL
                     AGLGVAKGDRVVIYMPMVPEAVIAMLATARLGAIHSVVFGGFASNELAKRLDDAKPKL
                     VVSASCGVEPGRTVLYKPLLDKAIEIAAHKPESCIILQRPLAAAQLHEGRDHDWAGLV
                     AEAKARGLAADCVPVAATDPLYILYTSGSTGVPKGVVRDNGGHMVALTWTMKNLYGVK
                     PGEVFWSASDIGWVVGHSYIVYGPLLAGNTTILYEGKPVGTPDAGAFWRVASEHKVAA
                     LFTAPTALRAIKKEDPHGTHLAKYDLSNFRALFLAGERADPDTVKWAED"
     gene            1272..2039
                     /locus_tag="VFY74_03895"
     CDS             1272..2039
                     /locus_tag="VFY74_03895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016734610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5930885.1"
                     /translation="MPGRLAGKIALISGGATGMGGASSRLFAAEGAKVGVVDRNEEAG
                     RAVVAEIERAGGTAMFALADVANEAQVNTAVAAVTAAFGAPNVLFNHAGSIIIKPFLE
                     TTLAEWEWLMAVNVTSMFLMTKAVLPGMIASGGGSIVCTSSISAIAATPREVVYNTSK
                     GACHMFARSIAVEYRDQNIRCNAVCPGFVRTPHGLREVDALNKLGVDMSDAALAVQQG
                     RICEPEEVARAALFLASDEASFINGAHLFVDNCFTAV"
     gene            complement(2078..>2363)
                     /locus_tag="VFY74_03900"
     CDS             complement(2078..>2363)
                     /locus_tag="VFY74_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5930886.1"
                     /translation="SLLLRIAASFEAGKRRAVVFPADPSGEQRNPVLWPRRLFPDLMR
                     LSGRGGAKDLLRAHRAESIAVAVDDPALLDDVDTQEDLEAARALLDKTRP"
BASE COUNT          404 a          761 c          789 g          409 t
ORIGIN      
        1 gatcctcggc ccacttcacg gtgtccggat cggcacgctc gccggcgagg aagagtgcac
       61 ggaagttcga cagatcgtat ttcgcgagat gcgtgccatg cgggtcttcc ttcttgatcg
      121 cgcggagcgc cgtcggcgcc gtgaagaggg ctgcaacctt gtgctcgctt gcgacgcgcc
      181 aaaacgcgcc ggcgtccggc gttccgacgg gcttgccttc gtacaggatg gtcgtgttcc
      241 cggcgagaag cggcccatag acaatataag agtggccgac gacccagccg atgtccgatg
      301 ccgaccagaa cacctcgccc ggcttgacgc cgtagaggtt cttcatcgtc cacgtgagcg
      361 ccaccatgtg accgccattg tcgcgcacga cacctttcgg cacgcccgtg ctgccggagg
      421 tgtagaggat gtagagcggg tcggtcgcgg cgacgggcac gcagtccgcc gcaagtccac
      481 gtgctttggc ttcggcgaca aggcctgccc aatcgtggtc gcggccttcg tgcaattgcg
      541 cggccgcgag aggacgctgg aggatgatgc agctctcggg cttgtgggcc gcaatctcga
      601 tggccttgtc gagaagcggc ttgtagagga cggtgcggcc cggctccaca ccgcatgagg
      661 cggacaccac cagcttcggc ttggcgtcgt cgagccgttt ggcaagctcg ttgctggcga
      721 agccgccgaa cacgaccgag tggatcgcgc cgagccgtgc cgtggccagc atggcgatca
      781 cggcctcggg caccatcggc atatagatga cgacgcggtc gcctttcgcg acgccgagcc
      841 ccgccaaaac ggctgcgaac gttgccacct cgtcgctaag ctcggaaaag gtgaatttcc
      901 gcttggtgtt cgtgaccggg ctatcgtaga tgagcgcagt ggcagcgccc tgccccgaat
      961 cgacgtgccg gtcgaggcag ttgtaagccg tgttgcaaag ggcgcccgga aaccagcggc
     1021 catattgtcc ggcgccttcg tcgaacactt tgtcgtaagg cttgatccaa tcgacggcgt
     1081 cggcggcgtc agcccagaag gcttcggggt cggccttcca gctctcgtag acgtgccggt
     1141 attcgctcat cgctaacccc gcctgattgc cttgagacga gagcgcttcc cgtcccgcgc
     1201 gccactttcg gccgcgcccg atgccgttct agaatagcgc aaccgtgtct gaggagaagg
     1261 atgaacgcac catgcccggc agactggcag gcaagatcgc attgatttcg ggcggcgcaa
     1321 ctggcatggg cggcgcctcg tcgaggctgt tcgcggcgga aggcgccaag gtcggggtcg
     1381 tcgaccgcaa cgaggaggcg gggcgtgccg tcgtggccga gatcgaacga gctggcggca
     1441 cggccatgtt cgcgctcgca gacgtcgcca acgaggcgca agtcaacacg gcggtcgccg
     1501 ccgtcacggc ggcattcggc gcgccgaacg tgctcttcaa tcacgcgggc tcaatcatca
     1561 tcaagccctt cctcgaaacg acgctcgcgg agtgggaatg gctgatggcg gtcaacgtca
     1621 cgagcatgtt cctcatgacc aaggcggtgc tgccggggat gatcgcttcc ggcggcgggt
     1681 cgatcgtctg cacatcgtcg atctcggcca tcgcggccac gccgcgcgaa gtcgtctaca
     1741 atacgtccaa gggcgcctgc catatgttcg cgcgctcgat cgcggtcgag tatcgcgacc
     1801 agaacatccg ctgcaacgcc gtctgcccgg gcttcgtgcg cacgccgcac ggccttcgcg
     1861 aggtcgacgc actgaacaag ctcggcgtcg acatgtcgga tgcggcgctt gccgttcaac
     1921 agggccgcat ctgcgagccc gaggaggttg cgcgcgcggc gctctttctc gcaagcgacg
     1981 aggcgagttt catcaacggc gcgcatctct tcgtcgacaa ctgcttcacc gccgtgtgag
     2041 ccagcgcgcc tcttccagcg ctgcgcgaaa gcgcagtcta gggcctggtc ttgtcgagca
     2101 gagcgcgcgc cgcttcgagg tcctcttgcg tgtcgacatc gtccaggagc gcgggatcat
     2161 cgacggcgac ggctatcgat tcggcgcggt gcgcgcgcaa caaatccttt gctccgccgc
     2221 ggccggagag cctcatcaga tcggggaaca agcgccgggg ccacagcacc gggttccgct
     2281 gctctccgga cgggtcggcg ggaaagacga cggcgcggcg cttgcccgcc tcgaaggaag
     2341 cggcaatgcg gagcagcaag gac
//
LOCUS       DASUQP010000087         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1128776, whole genome shotgun sequence.
ACCESSION   DASUQP010000087 DASUQP010000000
VERSION     DASUQP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128776"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..589)
                     /gene="zapA"
                     /locus_tag="VFY74_03905"
     CDS             complement(197..589)
                     /gene="zapA"
                     /locus_tag="VFY74_03905"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX5930887.1"
                     /translation="MGQVSVVLNGRTYRLECGEGEESHLIALTEYLGTHVEDMKRKFG
                     QVGDDRLILMASLLITDELWELKREMEQLKSALAVARRDRSVADESAKSAKTDLAQRI
                     DAVADRLELLNERFGTEVKAPVKARRRP"
     gene            complement(624..914)
                     /locus_tag="VFY74_03910"
     CDS             complement(624..914)
                     /locus_tag="VFY74_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HEX5930888.1"
                     /translation="MSQSDALERAAARLDLAVDQLETFLRQAFAEAEDGVSLAALREQ
                     VRFLTEDRDRLLRELEAERARVRGLSAANEEVSGRLEAVIVTLKDLMPANPR"
     gene            1141..1770
                     /locus_tag="VFY74_03915"
     CDS             1141..1770
                     /locus_tag="VFY74_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5930889.1"
                     /translation="MAEIKPHCRLYLQFPMQPPANLISGLAHVAANTDAACVLLCSDP
                     SPSNENPGPLVEFVQGSGLACLIAEDAAFASKLGADGVHLAADSTRYTEARKILGPDA
                     SLGAGCGLSRHDAMQLAGMGADYVAFGPADRRAIDTIDQYADLISWWSEIFVVPCVAW
                     NVASAEEAARCAACGADFVAPALSLWRRDDGPRLIAEIDRAVRGSRRAA"
     gene            1767..2684
                     /locus_tag="VFY74_03920"
     CDS             1767..2684
                     /locus_tag="VFY74_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5930890.1"
                     /translation="MTAYRVCAAILAIALAHALAPTQAGAAENPSYAAYAQGRYLTAL
                     KLAEQEAAQGSKEAYTLIGEIYSGGLGVAQDYGKAADAYAKASDLGDANAQLLLGLMV
                     AEGIGVKKDPRLAAGLFEKAAENGNPAAQYNLALVYLDGKGRPADEAKAAQWMEKAAA
                     AGNPAAQYDLGALYQFGRGVPIDKMKAAEWTGRAADAGLADAQVEYAVMLFKGQGVAV
                     DEARAAKLFRLAAEQGNAVAQNRLARLYANGVAVKADPVQAAKWHLLARGAGVSDFSL
                     DIVLSKLTPQERAKAEDAAENWRGERLSE"
     gene            2769..>3110
                     /locus_tag="VFY74_03925"
     CDS             2769..>3110
                     /locus_tag="VFY74_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5930891.1"
                     /translation="MKINGNEIRPGNVIQHKGGLWVAVKTQHVKPGKGPAYAQVELKN
                     AIDGTKLNERFRSSETVERVRLEQKDYQFLFAEGDMLTFMDMDTYDQIQVPADFLEER
                     AAFLQDGMKVSI"
BASE COUNT          570 a         1003 c          969 g          568 t
ORIGIN      
        1 ggaagttgga atggccaggc cgcgatggcc gtcggagcga ggatcccggg tgtccctaca
       61 tcagtaggtg cgcgccaaat ggcggagacc acggtccccg tcagggtcgg ggcccgggga
      121 ttgtgctcct aacgcacctc gcagcctgcc gtttcgacta tagcgaagga aatgcgatcc
      181 cgcgacagga tcgaagctaa ggccgccttc ttgccttgac cggcgccttc acctcggtgc
      241 cgaaccgctc gttgagaagt tccagccggt cggcgacggc gtcgatgcgc tgagcgagat
      301 cagtcttggc cgacttcgcc gattcatcgg caaccgaccg gtcgcgtcgc gccacggcaa
      361 gcgcggattt cagttgctcc atctcgcgct tgagctccca caattcgtca gtgatgagca
      421 gcgatgccat gagtatcaga cgatcgtccc ccacctgccc aaattttcgt ttcatgtctt
      481 cgacatgggt gccgagatat tccgtcagcg cgatgaggtg gctttcctcg ccctcgccgc
      541 attcgagccg ataggttcgg ccgttcagaa cgacgctgac ttgacccatg acaccccccg
      601 gtacacgtgc tcccggcaat gatctacctg gggttggctg gcatcaagtc cttgagcgtc
      661 acaatcaccg cctcaagtct gccggaaacc tcttcgttgg cggccgacag tccgcgcacc
      721 cgggcccttt cggcctcgag ctcgcggagc aaccggtcgc ggtcttcggt gaggaaccgg
      781 acctgctcgc gcaacgccgc aagcgaaacg ccgtcctcgg cttcggcaaa ggcctgccgc
      841 aagaaggtct caagctgatc gacagccaag tcgagccgcg cggcagcccg ctccagggcg
      901 tcggattggc tcatcgctgt actctccccc tgaaacgccg ttcttcttgt cagcctgact
      961 caagctggtg accctccggc ttgatgtcaa tgttatggcg cccgacttaa acgacgaacc
     1021 tcgtgccttt gttccgcccg ctgacattga cagacaagcg agccccatga tttgagacgg
     1081 gctctgtgtt gagccttcga tcgattcgac gggagcgagg aagcgggagt ccagcggagg
     1141 atggccgaaa tcaagcctca ttgccggctt tatcttcagt ttcccatgca gcctccggcc
     1201 aacctcatca gcgggcttgc ccatgttgcc gccaacaccg acgcagcttg cgtcctactc
     1261 tgttccgacc cctcgccttc caatgagaac ccgggcccac tggttgaatt cgtgcaagga
     1321 agcgggctcg cctgcctcat cgcggaggat gccgcgtttg cctcaaaatt aggcgccgat
     1381 ggcgttcatc ttgccgccga ttcaactcgc tatactgagg cgcgcaaaat tctcggcccg
     1441 gatgcaagtc ttggcgcggg atgcggcttg agccgtcacg acgccatgca gcttgccggg
     1501 atgggcgccg attacgtcgc cttcggtcct gcggacagga gggctattga cacgatcgat
     1561 cagtatgctg atctaattag ttggtggtct gagatttttg tcgtgccgtg cgtcgcctgg
     1621 aatgtcgcaa gcgcagagga agcggcgcgg tgtgctgctt gcggcgctga tttcgttgca
     1681 cccgcgctca gcctctggcg gcgcgacgat ggtcctcggc tgatcgccga gatcgatcgt
     1741 gccgttcgcg ggtcgcggag ggccgcttga ccgcttatcg cgtatgcgct gccatccttg
     1801 caatcgcgct ggcccatgcg cttgccccga cgcaagccgg cgcggcggaa aacccttcat
     1861 acgcggccta cgcccaaggc cgctacctga cggcgctcaa gctcgcagaa caagaggcgg
     1921 cacaaggctc gaaggaagcc tacacgctca taggcgagat ctattccggg ggactcggcg
     1981 ttgcgcagga ctatggcaag gcggccgacg cctatgccaa ggcctccgat ctaggcgacg
     2041 ccaatgccca gctcttgctg gggcttatgg tcgccgaggg gatcggcgtg aagaaggacc
     2101 ctcgcctcgc cgccggcctg ttcgagaaag cggcggagaa cggcaatccc gccgcgcaat
     2161 ataatctcgc gctcgtctat ctcgatggca aggggcgccc cgccgacgaa gcgaaggccg
     2221 cccaatggat ggaaaaggcc gccgcggcgg gcaatcccgc cgcgcaatac gacctcggcg
     2281 cgctttacca attcggccgc ggcgtgccga tcgacaagat gaaggcggcg gagtggacgg
     2341 gccgcgcggc cgacgcgggc ctcgccgatg cccaggtcga gtacgccgtc atgctgttca
     2401 agggccaagg cgtggcggtg gatgaggccc gcgccgccaa gctcttccgg cttgccgccg
     2461 agcaaggcaa tgccgtcgcc cagaaccggc tcgcgcggct ctacgccaat ggcgtggccg
     2521 tcaaggccga tcccgtgcag gccgcgaaat ggcatttgct ggcgcgcggc gccggcgtca
     2581 gcgatttctc cctcgacatc gtgttgtcca agctcacgcc gcaggagcgc gccaaggcgg
     2641 aggacgcggc tgagaattgg cggggcgagc gcttgagcga gtagccgtcg ctccggccgg
     2701 tcgcacttga caccggttcg cccgcgcgtc ttaaaggcgc cgaccttccg atttcgccaa
     2761 ggcgtcccat gaagatcaat ggcaacgaga taaggcccgg caatgtgatc cagcacaaag
     2821 gcgggctctg ggtcgcggtg aagacgcagc atgtcaagcc cggcaaggga ccggcctacg
     2881 cgcaagtcga gctcaagaac gcgatcgacg gtaccaagct caacgagcgc ttccgctcct
     2941 ccgagacggt cgagcgcgtg aggctcgagc aaaaggacta ccagtttctc tttgccgaag
     3001 gcgacatgct gaccttcatg gacatggata cctacgacca gatccaggtc ccggccgact
     3061 tcctcgaaga gcgcgcggcc ttcctgcaag acggcatgaa ggtgtcgatc
//
LOCUS       DASUQP010000088         6250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1674260, whole genome shotgun sequence.
ACCESSION   DASUQP010000088 DASUQP010000000
VERSION     DASUQP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674260"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..607)
                     /locus_tag="VFY74_03930"
     CDS             complement(185..607)
                     /locus_tag="VFY74_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX5930892.1"
                     /translation="MAYDENNVFAKVLRGEIPCHKIYEDADTLAFLDIMPRADGHALV
                     VTKEKARDFFDISPVALAKLMAVVQKLAPKIKEAMGADGVLIQQFNGAAAGQTVFHLH
                     VHIIPRKEGEALKPHAGKMGDQTALAATAEKIRKTLKG"
     gene            complement(728..1957)
                     /locus_tag="VFY74_03935"
     CDS             complement(728..1957)
                     /locus_tag="VFY74_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5930893.1"
                     /translation="MGDIEQTPTRAESERASEAFSLRIAARIGDVAACDWDACAQGGA
                     ATESPATDRTNPFISHAFLMALEGSGSATRATGWLPHHLLLEDKSGRLIGCMPCYVKS
                     HSLGEYVFDHGWADAYARAGGRYYPKLQVSVPFTPVTGRRLLVRDGENRAAHEALLLQ
                     AAIQVTDRLGLSSLHITFPTREEWELAGEIGYLKRTDQQFHWRNEGYATLDDFLAALA
                     SRKRKVIRKERREALLDGIAIEWVTGSDLTEARLDAFFAFYMDTGSRKWGSPYLTRDC
                     FSLLAESMADRMVLILAKRSGRYVAGALNFLGEDALYGRYWGGIGHHPFLHFEVCYYQ
                     AIEYAIANKLARVEAGAQGAHKLARGYLPVETYSAHYIADAGLRRAVADYLKRERRAV
                     SREIALLATESPYRGKA"
     gene            complement(1962..2705)
                     /locus_tag="VFY74_03940"
     CDS             complement(1962..2705)
                     /locus_tag="VFY74_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5930894.1"
                     /translation="MAELYWLKRPIAHRGLHNAAKGIIENSVSAVSAAMGKGLAVEVD
                     VQCAAGRMPIVFHDATLDRLTAETGPVAARDVDTLSGIPLRNSDDRILSLPVLLSLVD
                     GHVPLLLEVKSTWTREGTFEKNIAAMLASYKGPVAVMSFDPYALASFGEVAPELPRGL
                     VAERFDKPGDWPHLTAKQRFTMRHLLSAAIARPNFIAYDIAALPAVAPLVARTLFGLP
                     LLTWTVRTEEERERAMRYADAMIFEGIKP"
     gene            complement(2728..3192)
                     /locus_tag="VFY74_03945"
     CDS             complement(2728..3192)
                     /locus_tag="VFY74_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5930895.1"
                     /translation="MAGEIEARLKALGLVLPEARAAIGSYAPFLHINGQLFISGQLPL
                     VDGKIAVAGKVGAELDLGRGQEAARLCALNILTQAKAALRDLDRIVQLVRLNGFVNAA
                     PDFADHPKVLNGASDLMVEILGNKGIHTRIAVGCASLPLNAAVEVDAIFAID"
     gene            3370..4236
                     /locus_tag="VFY74_03950"
     CDS             3370..4236
                     /locus_tag="VFY74_03950"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HEX5930896.1"
                     /translation="MGGFSQRFLLTLLVAGPLAGTPNGSAQAIAANEANKLVPHRAVY
                     EMKLDDTRSATGITGITGIDGRMVFEFTGSECDGYSLNMRMVTQMTDSQDQTNLTDLR
                     SSTWEQGDGRKFRFQSSQYVNEKQGDVTMGRAVRETPNEAVRIKLSQPAQAELKLSGP
                     VLFPTQHSLALIDKARAGQTLFQARVYDGSEKGRKVYETTAVIGKIVPPGADPKLEIV
                     AKDKGLGELSSWPVSIGYFEEQTGDLTPSYQIDFRLYENGVSRELLIDYGSFSVYGTL
                     TALEYLKASECH"
     gene            4470..4844
                     /locus_tag="VFY74_03955"
     CDS             4470..4844
                     /locus_tag="VFY74_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5930897.1"
                     /translation="MTKTILIVEDNELNMKLFHDLLDAHGYKTLQTRNGMEALALARE
                     HRPDLILMDIQLPEVSGLEVTKWLKEDDQLRKIPVVAVTAFAMKGDEERIREGGCEAY
                     ISKPISVSMFLDTIRQFIGEAR"
     gene            complement(5398..5565)
                     /gene="rpmG"
                     /locus_tag="VFY74_03960"
     CDS             complement(5398..5565)
                     /gene="rpmG"
                     /locus_tag="VFY74_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5930898.1"
                     /translation="MAKPVTLKIKLVSSAGTGYYYVTKKNPRNQTEKLVFKKYDPVAR
                     KHVEFKEAKIK"
     gene            complement(5659..>6250)
                     /locus_tag="VFY74_03965"
     CDS             complement(5659..>6250)
                     /locus_tag="VFY74_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5930899.1"
                     /translation="YAHFTSPIRRYADLIVHRALARALGMGNDGLSDTEMGELEEIAR
                     HISETERRAMAAERETADRLIAAYLADRIGAAFAARVSGLVRSGLFVRLVETGADGFV
                     PASSIGREYYFHDEVRHALVGEDTGLAYRLGDPVEVRLVEAIPTAGALRFEMLSEGRP
                     IRPRPKGARKPSDRGKAQRYKGPRGQGRGRGRKKRR"
BASE COUNT         1135 a         1937 c         2002 g         1176 t
ORIGIN      
        1 tgaggagcgc ccggcggctc gccaagggcg cgcctcgaag cacgcacaca atttcttgct
       61 gccgcggttc tcttttctaa cctcccccct cgtggggagg tccggcggcg cgcgaagcca
      121 caacgtggct acgcgcgacg ctggggtggg gcctgcgcgg gcatgacatt tgtgtggtgt
      181 aagattaccc tttcagtgtc ttccggattt tttccgcggt ggcggcgagc gccgtctggt
      241 cccccatttt gcccgcgtgc ggctttagcg cctcgccctc tttgcgcggg atgatgtgga
      301 cgtgcaggtg gaacaccgtc tggcctgcgg ccgcgccgtt gaattgctgg atcaggacgc
      361 cgtccgcgcc catggcctct ttgatcttcg gcgcgagctt ctgcacgacc gccatcagct
      421 tggcgagagc gactggcgaa atgtcgaaaa agtcgcgcgc cttctccttg gtgacgacca
      481 gagcgtgccc gtcggcccgc ggcatgatgt cgaggaaggc gagcgtgtcg gcgtcctcgt
      541 agatcttatg gcaggggatc tcgccgcgga gcaccttggc gaagacgttg ttctcatcgt
      601 aggccattgc agccttctag cccttcattg tcatcgcccg gtaggaccgg gcgatccagc
      661 aatgcgcgcg ttaggttata gcgaagcacc ggggttactg gatacaagct ttcgcctgta
      721 tgccgcctta tgccttgccg cggtagggcg attccgtggc aaggagcgcg atctcgcgcg
      781 agaccgcgcg gcgctcccgt ttcagatagt cggcgacggc gcggcgcaag ccggcatcgg
      841 cgatgtaatg cgcgctatag gtctcgaccg gcagatagcc tcgggcgagc ttatgcgcgc
      901 cttgagcgcc cgcctctacg cgggcgagct tgttagcgat ggcgtattcg atggcctgat
      961 aatagcagac ctcgaaatgg aggaaggggt ggtgcccgat gccgccccag taacggccgt
     1021 agagtgcatc ctcgcccagg aagttgagcg cgcccgcgac atagcgcccg gagcgcttgg
     1081 cgagaatgag caccatccgg tcggccatgc tctcggcgag caggctgaag cagtcccgcg
     1141 tcagataggg cgagccccat ttgcgcgagc ccgtgtccat gtagaaggca aagaaggcgt
     1201 cgagacgcgc ctcggtgagg tcgcttcccg tcacccattc gatggcgatg ccgtcaagaa
     1261 gcgcctcgcg ccgctccttt ctgatgacct tccgcttgcg cgaggcaagc gccgccagga
     1321 aatcgtcgag ggtcgcatag ccctcgttcc gccagtggaa ttgctggtcg gttcgcttga
     1381 ggtagccgat ctcgcccgca agctcccatt cctcccgcgt cgggaaggtg atgtgcagag
     1441 aagagaggcc gagccggtcc gtcacctgga tcgccgcctg cagcaggagc gcttcatgcg
     1501 cggctcggtt ctccccatcc ctgacaagca ggcgccgccc ggtgacgggc gtgaagggaa
     1561 ccgatacctg aagctttgga tagtagcggc cgccggcgcg cgcataagcg tcagcccagc
     1621 cgtgatcgaa cacgtactcc ccgagcgaat gcgatttgac gtagcagggc atgcagccga
     1681 tcagtctgcc cgacttgtct tcgaggagaa ggtgatgcgg cagccagccg gtcgctcggg
     1741 ttgccgaccc gctcccctct aaggccatca gaaaagcgtg tgaaatgaaa gggttagtcc
     1801 gatccgttgc gggcgattcc gttgcggcgc ccccttgcgc gcaggcgtcc caatcgcatg
     1861 cggcgacatc gccgatgcgc gcggcaatgc gcaaggaaaa ggcctcgctc gcgcgttcgg
     1921 attcggcacg ggtcggggtc tgttcgatat cgcccatcgc tttatggctt gatcccttca
     1981 aagatcatgg catctgcata gcgcatggcg cgttcgcgct cctcctcggt ccgcaccgtc
     2041 catgtcaaca gcggtaaccc gaacagggtt cgcgcaacga gcggcgcgac cgcgggcagc
     2101 gccgcgatat cgtaggcaat gaagttgggc cgggcgatgg cggcgctcag gagatggcgc
     2161 atggtaaagc gctgcttggc cgtgagatgc ggccaatcgc cgggcttgtc gaaccgctcg
     2221 gccaccagtc cgcgcggaag ctcgggggcg acctcgccaa acgacgccag cgcgtaaggg
     2281 tcgaacgaca tcacggccac gggccctttg taggaggcga gcatggcggc aatgttcttc
     2341 tcgaacgtgc cctcgcgcgt ccaggtgctc ttcacttcga gcagcagcgg cacgtggccg
     2401 tcgacgaggc tgagcaggac gggaagcgaa aggatgcggt catcgctgtt gcgcaaggga
     2461 atgccggaca gagtgtcgac gtcgcgcgcg gcgaccgggc cggtctcggc cgtgagccgg
     2521 tcgagtgtcg cgtcgtgaaa gacgatcggc atgcggcccg cggcgcattg cacgtcgact
     2581 tcaacggcca ggcccttgcc catggcggcg ctgacggccg agacgctgtt ctcgatgatg
     2641 cccttggccg cattgtgcaa gccgcgatgg gcaatgggcc gcttcaacca atagagctcg
     2701 gccatgagag acgctcggcc acggggctta gtctatcgca aaaatcgcat cgacctcgac
     2761 tgcggcgttg agcggcaggc tcgcgcaacc aacggcgatc cgggtatgga tccctttatt
     2821 gccgagaatc tcgaccatga ggtcggacgc gccgttgagc accttggggt ggtcggcgaa
     2881 atcgggagcg gcgttgacga agccgttgag gcgcacaagc tggacgatgc ggtcgagatc
     2941 gcgcagcgcg gctttcgctt gcgtcaggat gttgagagcg caaagccgcg ccgcctcctg
     3001 gccgcgtccg aggtcgagtt cggcaccgac cttgccggca acggcaatct tgccgtcgac
     3061 gaggggcagc tggccggaaa tgaaaagctg accgttgatg tgaagaaagg gagcatagct
     3121 gccgatcgcc gctcttgcct cgggaaggac gagcccgagt gccttcagcc gtgcttcgat
     3181 ctcacccgcc atggtcgtcc tcttcagcct tgagtgctct gccgcgaggg cctaaaccat
     3241 tcagcaaatc cgatcgccgc gcaagaagcg gtcatgacct cctcttgtcc gtcattgcgg
     3301 ttggtgcgaa gaatcgcttg gccacggggc aacgggctaa aaaccctggc caagggttgg
     3361 gaatagcata tgggcggttt cagccaacgg tttctactga cgttgctcgt ggcagggccg
     3421 ctcgccggaa cgcctaacgg ttccgctcag gcgatcgcgg caaacgaggc gaacaagctc
     3481 gtgccccatc gggccgtcta tgagatgaag ctcgacgata cgcgctcggc cacgggcatt
     3541 accggcatta ccggcatcga tggccgcatg gtgttcgagt tcaccggctc cgagtgcgac
     3601 ggctattcgc tcaatatgcg catggtcacg cagatgaccg attcccagga tcagaccaac
     3661 ctgaccgatc tccgctcctc gacttgggag cagggcgacg ggcggaagtt ccgttttcaa
     3721 tcgtcacaat acgtgaacga aaagcagggc gacgtgacca tggggcgcgc ggtgcgcgaa
     3781 acccccaacg aagcggtcag aatcaagctg agccagcccg cgcaggcgga gctcaagctt
     3841 tctggcccgg tgctgttccc gacgcagcat agccttgcct tgatcgataa ggcgcgcgcc
     3901 ggtcagacgc tgttccaggc gcgggtctac gacggctcgg agaagggaag aaaagtctac
     3961 gagaccacgg ccgtcatcgg aaagattgtg ccgcccggcg ccgaccccaa gctcgagatc
     4021 gtcgccaagg acaagggact gggcgagctt agctcgtggc ccgtgtcgat cggctatttc
     4081 gaggaacaga ccggcgatct cacgccgagc tatcagatcg attttcggct ctacgaaaac
     4141 ggcgtcagcc gcgagctcct gatcgactat ggcagcttct cggtctacgg gaccttgacc
     4201 gcgctcgagt atctcaaagc ctcagagtgt cactgagggg cctgagagac ccctcgcggc
     4261 cccggagccg cggcaccttg ccatgcccca aagcgtttgc cgcccatcga aagaaattcg
     4321 ttagaggttt ccggcgaaac tcgcccggcg cttgaaggac gggagcaggg accatgtttt
     4381 ggaggcttaa atcggctccg agcgccatgg cgcctttgca cgagatcgcc catcatgggg
     4441 ggaccgaatc gcagccgcgg ccgagcacca tgaccaagac cattctgatc gtcgaggaca
     4501 acgagctgaa catgaagctg tttcatgatc tgctcgatgc ccatggctac aagacactcc
     4561 agacgcggaa cggtatggaa gcactcgcgc ttgcccgcga gcaccgtccc gatcttatct
     4621 tgatggacat ccagctcccc gaggtctcgg ggcttgaagt gaccaaatgg ctgaaagagg
     4681 acgaccagct ccgcaaaatt ccggtcgtcg cggtcacggc cttcgccatg aagggcgatg
     4741 aggagcgcat ccgcgaaggc ggttgcgagg cctatatctc gaagccgatc tcggtctcga
     4801 tgttcctcga cacgatcagg cagttcatcg gcgaggcgcg gtaaggacaa caaccgcatg
     4861 cgtgctcttc ggcacaaaag ccgctgctaa gagccgccgc cgagagggaa cccaacggcc
     4921 gattccgtgg actgagcatc gcgcctaagt ttatgtgcaa cctgagcgct taaccttaac
     4981 actttacaaa gcttagcatt ttatcgtatt aagcgaggaa taagattgat ttcatcccgt
     5041 ctttgcctgt tttatgatca gactcgcagt cgaacgcgac gggaggcaat tggggcttcg
     5101 gatttgcggc gccaagttcc gagcagctct ggaccctcta ttcccctcgt cctaagagtt
     5161 aaccctgcgg aatgctcagg tccgccgcat ctcctgggtc cgtagggttc ggccggccgg
     5221 agcgcggttg agtggcctcc tcgttagcgc cgcgtttcgg ccggccacca gttcacacaa
     5281 cccttttcgt gggtgcccgt ggttgtcttg ccggccgttc gcacataagg ccgctcacag
     5341 cctcggccgg gccgtcgcgc tgtctcgcag atcgcaaggg ctaccctccg atcttgccta
     5401 cttgattttg gcttccttga attcgacgtg cttgcgcgca acggggtcgt atttcttgaa
     5461 tacgagcttc tcggtctgat tgcgcgggtt ctttttggtg acgtagtagt agcccgtccc
     5521 ggctgaactc acgagcttga tcttcagggt gacaggcttg gccatctcgt cctcgacaag
     5581 gctgggggca aatttggcgc gaccctactc gctcggagcc ggaagtcaag gcggcgcagg
     5641 ctatggtccg cgcgtaggct aacgtcgctt cttgcgaccg cgtccacgcc cttgcccgcg
     5701 cgggcccttg tagcgttgcg cctttcccct gtcgctcggc ttccgtgcgc ccttcggtcg
     5761 cgggcgtatg gggcgtcctt cgctcagcat ctcgaaccgc agcgcgccgg cagtcgggat
     5821 cgcctcgaca agcctgacct cgacgggatc gccgagcctg taggcgaggc ccgtatcctc
     5881 gccgacgagc gcgtgccgga cctcgtcatg aaagtagtat tcgcgcccga tcgacgaggc
     5941 cgggacgaaa ccgtcggcac ccgtctcgac gagcctgacg aacaggccgc tgcggacgag
     6001 gccagagacc cgcgccgcaa acgccgcccc gatccggtcg gcgagatagg ccgcgatgag
     6061 gcggtccgcg gtctcccgtt cggccgccat ggccctgcgc tcggtttcgg agatatgacg
     6121 cgcgatctcc tcaagctcgc ccatctcggt gtcgctcaag ccgtcgttcc ccatccccaa
     6181 ggcccgcgcc agcgcgcggt ggacgatgag atcggcatag cggcggatcg gcgaggtgaa
     6241 atgggcgtag
//
LOCUS       DASUQP010000089        20125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_856978,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000089 DASUQP010000000
VERSION     DASUQP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 20125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20125
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856978"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VFY74_03970"
     CDS             complement(<1..1210)
                     /locus_tag="VFY74_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5930900.1"
                     /translation="MHVRRALLAAVPMLAFVGLELALISASFAGATETAPAVANDARV
                     AGDRERTRFIADLSRKVDVHVFALGNPYRVIVDAPDVNFQMPDGIGKQKRGLVTAYRY
                     GLFAPGKARIVIDVAGPFLIDKAFVLEPRDDQPARLVVDLVPTDEKTFLAKQKEARAQ
                     AEKEMPIVAPQTPSRPPYAKPVVVLDAGHGGLDPGTKSASGITEKEVVLTFARALKAK
                     LEAAGRYEVYLTREDDRFLALRERVEFAQKKGAGLFLSIHADYFPQEIDKTTGATVYT
                     LSEKASDEEARALAAKENFSDALAGIELPSDSDEVLANILIDLAQRETQNRSAVFARS
                     IVGELAARGALHTRKIRSAGFRVLKAPDVPSVLLELGFLSNPDDEKLLTSEAWRDRTA
                     EAVVKSIDAYF"
     gene            1811..4402
                     /locus_tag="VFY74_03975"
     CDS             1811..4402
                     /locus_tag="VFY74_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5930901.1"
                     /translation="MASKMLIDAAHPEETRVVVVRGNRVEEFDYESANKKQLRGNIYL
                     AKVTRVEPSLQAAFVDYGGNRHGFLAFTEIHPDYYQIPVADRQALLEEEAAEESRASA
                     AEAAEDEAAAGRRHGRRRRGRGRHEGQGRRDAPSPSAAPAEPMPIAKLEHDDDDEIDT
                     TPREDPQVVAMEATDLIHKVSEPAEEPDAETEERHFAAEPEPGDAGEDEDEAEPGEIV
                     GQPVALKEGNGETAVEAVESVGSEDVLEEVPERARRRRGRPYKIQEVIRRRQIILVQV
                     VKEERGNKGAALTTYLSLAGRYTVLMPNTARGGGISRKITNPTDRKRLREIAGELEVP
                     EGMGLIIRTAGAQRTKTEIKRDYDYLLRLWENVRDLTLKSTAPALVYEEGSLIKRSIR
                     DLYNKDIDEVLVAGDDAYREAKDFMRMLMPSHAKNVKPYKEPEPVFIGYQVERQLAAM
                     FSPQVVLKSGGYIVINQTEALVSIDVNSGKATREHNIEDTALKTNLEAAEEIARQLRL
                     RDLAGLIVIDFIDMDERRNNRLVERRLKEALRHDRARIQVGRISHFGLLEMSRQRLRT
                     GMLEGSTKPCPMCQGTGMVRSVESVALDILRSIEDRLITDGVVPLVATTAVDVALYIL
                     NQKRIHLNDIQQRYRVAITVNADEDMHISQFVIERAAEGEIANGSVPVHMDWAHHQET
                     PARPAAAEAEAEPPRSRSRRRRRRRRSDGGEFHRPMHSAAAEPEEEMEERAEQPRTTG
                     AAAASGGEAKEEGGAQARKGPRRRGRRGGRRGRSGQRPQGAPGEEGPSDSRIGVRGER
                     AATSPVDASKNGIGGPRSDEEASAQEPAPVLETVTTASEREPPSGAHAETGGEPEPPR
                     RRGWWQR"
     gene            4663..6027
                     /locus_tag="VFY74_03980"
     CDS             4663..6027
                     /locus_tag="VFY74_03980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5930902.1"
                     /translation="MFVGALSAAQIVATTADAKGLIRDAEIEALIRIYAKPILNAAGI
                     GSQGIRIHLVNDLSFNAFVVDGHNIFMHAGGLTSAKTPNQVIGVLAHETGHITGGHLA
                     RLRTQVSRAKSAALMMQIIGLAAMVGGAFAGAPGMGQAGMGVATGGMDAAFRTLLAYR
                     QNEESSADQAAVTFLNATKQSGRGMIETFEFMGSKLVGVQGINPYLQSHPLPQQRITN
                     LRALVESSPYYNNVDPPELQFRHDLMKAKLFGFLDDPATVFNRYPQTDQSLPALYARA
                     IAVYRKSGMKAAAPLIDQLIAAKPDWPYFYELKGQFLFESGNPAAAVPPLREAVRLSP
                     NEPLLRVMLGQALLGANDRKLVDEAITNLRTALTREDNSAMGYRQLAAAYARKAEAAQ
                     AVGGKKQFMAQAALASAEAYFYEGQLKLAKQQAKRARDGFIDGTPNWIKADDILAFEV
                     PQTN"
     gene            6052..6870
                     /locus_tag="VFY74_03985"
     CDS             6052..6870
                     /locus_tag="VFY74_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5930903.1"
                     /translation="MTPKTGLATALVAAIVAALVTAATLLVVWRAAPGVVEVPVVKVP
                     RAALDEKTIIDVVRDYLTKNPEILVEMTTELDKRQQAAESAKQQKVIGENADTIFRSP
                     LSYAAGNANGDVTVVEFFDYNCGYCRRALPDVVKLIDTDDKVRVVFKELPIFGEESEW
                     AAKAALAAGKQGKYFEMHQKLYAAPGKSDKEKALRVAAEIGLDVPQLEKDMEDPSIKQ
                     ALEETKELATKLGLQGTPLYLIGDRMIPGAPDDLYDQLVAKVAEIREKGCKVTC"
     gene            7020..7472
                     /gene="aroQ"
                     /locus_tag="VFY74_03990"
     CDS             7020..7472
                     /gene="aroQ"
                     /locus_tag="VFY74_03990"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5930904.1"
                     /translation="MAKPIYVLNGPNLNLLGTREPEVYGRETLDDLRAQCERKAEALG
                     FSLVFRQSNHEGELVAWVQEARTEAAGLIVNAGAFTHTSIALLDALLACTVPAVEVHL
                     SNIFAREPFRHHSYISRAVKGVICGFGATGYELAIEALTATLAPAKPK"
     gene            7498..7992
                     /gene="accB"
                     /locus_tag="VFY74_03995"
     CDS             7498..7992
                     /gene="accB"
                     /locus_tag="VFY74_03995"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5930905.1"
                     /translation="MSKQGGSFDKDLIRELAALLEETGLTEIEVEHDGRRIRVARGVN
                     LTTTLTTDLPQAEPRESARAKGDGPKPAKRAAGQGEEFIVHPGTVTSPMVGTAYRAPE
                     PGAPPFIEIGSKVTAGQTLLIIEAMKTMNQIPSPRAGTVIAILVEDGQPVEYGEPLAV
                     IEQR"
     gene            8003..9358
                     /gene="accC"
                     /locus_tag="VFY74_04000"
     CDS             8003..9358
                     /gene="accC"
                     /locus_tag="VFY74_04000"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5930906.1"
                     /translation="MFDKILIANRGEIALRVERACKELGVATVAVHSTADADAMHVRL
                     ADESVCIGAPPAKDSYLNIPAIVAACEITGADAVHPGYGFLAENARFAEILEEHEITF
                     IGPTAEHVQLMGDKIQAKIAAQQIGIPVVPGSGPVDDFDEAKRAAREIGYPVLVKAAA
                     GGGGRGMKLALSEDELEPALLTARNEAKAAFGDSTVYLEKYLTRPRHIEVQVLGDGRG
                     NAIHLGERDCSLQRRHQKLWEEAPSPALNENERVRIGEIVAKAMQALSYRGVGTVEFL
                     YEAGAFHFIEMNTRLQVEHTVTEMITGIDLVIEQIRIAAGAPLSLKQEDIPFNGHAIE
                     CRINAENPQTFRPSPGTITHFHTPGGLGVRVDSGAYAGYTIPPYYDSLIGKLIVHART
                     RNECLMRLKRALGEFVVDGIETTIPLFTSLIAEPDIVNGNYDIHWLEKHLAKHGTRGK
                     R"
     gene            9358..9948
                     /gene="aat"
                     /locus_tag="VFY74_04005"
                     /pseudo
     CDS             9358..9948
                     /gene="aat"
                     /locus_tag="VFY74_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084429.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
     gene            complement(9924..10433)
                     /locus_tag="VFY74_04010"
     CDS             complement(9924..10433)
                     /locus_tag="VFY74_04010"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEX5930907.1"
                     /translation="MVRNPIAVFAALDKVTGRISRLEIPINNTVEFGALKVTPRVCDT
                     RPPTEQPHTASFVEVDEVKLTGEVQRIFTGWMLAESPGLHAVEHPVFDVWLTNCKTNA
                     PPAPSGNAANAADPGAAPAAEAPPGAPPAEAPPGEAPADAAAAPPPPEPAEPLPWAKP
                     PPNADSSNR"
     gene            10387..10563
                     /locus_tag="VFY74_04015"
     CDS             10387..10563
                     /locus_tag="VFY74_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930908.1"
                     /translation="MTLSRAAKTAIGFLTISAPAGPGAKPKARAKESKERGSQEAGRR
                     VIPMLARSAMPHTS"
     gene            complement(10696..11496)
                     /locus_tag="VFY74_04020"
     CDS             complement(10696..11496)
                     /locus_tag="VFY74_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5930909.1"
                     /translation="MAAQLTEPSALDAVPPKAVQRWSASDYAKNGRFVHELAGPVFGM
                     LAAKAGERILDLGCGDGALTAEIRAVGADVLGVDLSEELLAVARMKGLPVRKLDGHAL
                     DFVSEFDAVFSNAALHWMRKPKLVIEGVARALRPGGRFVGELGGHGNVAAIATAIRAV
                     GALRGGDPSVTAPWFFPTPEEYTRLLAEGGFSVKEIVLVPRPTLLKTGMEGWLRTFGR
                     SFFDQFPEPLRGEVVGEVVELLRPSLCDTEGRWTADHIRLRFHAVRRD"
     gene            complement(11481..11858)
                     /locus_tag="VFY74_04025"
     CDS             complement(11481..11858)
                     /locus_tag="VFY74_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEX5930910.1"
                     /translation="MGFVTELFAWWTGNTIGTRLYTWRKGERVGEDGLGNVYYRERGG
                     KRRWVIYRTLAEPSQVPPEWHAWLHHTVDVPPTEEDYTPRPWEQAHRPNLTGTYEAYR
                     PPGSTLRPGAAKPPLDYEPWQPN"
     gene            12239..15943
                     /locus_tag="VFY74_04030"
     CDS             12239..15943
                     /locus_tag="VFY74_04030"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX5930911.1"
                     /translation="MRIERRFTEAGKSPYDAIPFRESGSEIRNPDGSVVFSLARFQVP
                     ERWSQVAADILAQKYFRKAGVPAKAKRVEETSVPSWLWRSVPDEAALAALPEAERYGG
                     ETDARQVFDRLAGTWTYWGWKGGYFTSEEDGQAFFDELRYMLAMQMGAPNSPQWFNTG
                     LYWAYGIDGPSQGHYYVDFETGEVKASTSAYERPQPHACFIQSIDDDLVNEGGIMDLW
                     VREARLFKYGSGTGTNFSRLRGENEPLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVVVDIDHPDIEDYINWKVTEEQKVAALVTGSKILSERLNAILKACVNCEGDGGD
                     CFDPNRNPALKREVRAARKAKVPDNAIRRVIQFARQGYTAIQFPAYDTDWDSEAYRTV
                     SGQNSNNSVRVTDDFLRAVEADGDWDLTYRRNGAVAKTVKARALFEQIGYAAWACADP
                     GIQFHTTINDWHTCPEAGPIKASNPCSEYMFLDDTACNLASLNLMAFRTEDGSFDIEA
                     YEHAIRLWTVVLEIAVVMAQFPSRRIAQLSYDYRTLGLGYANIGGLLMAAGVPYDSPQ
                     GRALCGALSAIMTGISYATSAEMARELGPFPGYAANREAMLRVMRNHARAAAGKPAGY
                     EGLDTAPVPLDHASCPDPRLLAHATHAWEKAIELGRKHGYRNAQATVIAPTGTIGLVM
                     DCDTTGIEPDFALVKFKKLAGGGYFKIINRMVPEALRGLGYPDEAIKRIVEYAVGHGT
                     LAGAAGVNHEALAAKGFTPEKIAAVEKAVASAFDIKFVFNRWTLGEDFLTGTLGLSPE
                     RIDQPDFDLLAEIGFGKREIEAANEYACGAMTLEGAPGLNPEHLPVFDCASPCGRKGK
                     RFLSVESHIRMMAAAQPFISGAISKTINMPNDATVEDCKEAYLLSWRLALKANALYRD
                     GSKLSQPLNAQLLADEEDEQEEVLAAVVSDQPQVQRAAVIAERIVERVVERMRARAER
                     ERLPDRRKGYTQKAVVGGHKVYLRTGEYADGRLGEIFIDMHKEGAAFRSLMNNFAIAV
                     SVGLQYGVPLEEFVDAFTFTRFEPAGPVRGNEAIKNATSILDYLFRELAVSYLGRYEL
                     AHVDPSEIGSTTLGSGDAGEGKRSTEALVSPGYTRGRLSERKLHAPSKAQSTGAHARA
                     LQADARDDAQPRTTSFAVPSSQLPSAETTEQVARAVETLAERGATELVAEARIKGYEG
                     EACGECGNFTLVRNGTCLKCDTCGATSGCS"
     gene            15977..16315
                     /locus_tag="VFY74_04035"
     CDS             15977..16315
                     /locus_tag="VFY74_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930912.1"
                     /translation="MHYGSSEEKDSEEEAGPQDRGGEKVVGEEDRQRPAQEEIGLLRG
                     AGLVPAPPLFSFQIKRLFGPSGAPQSAALAGFLPRRLPPFLASRIDAPALRLPVHTHV
                     ALRDVNENER"
     gene            complement(16446..17213)
                     /locus_tag="VFY74_04040"
     CDS             complement(16446..17213)
                     /locus_tag="VFY74_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX5930913.1"
                     /translation="MTSEQNQRTVTAALLVIGEEILSGRTKDENIGYIAAYLTRIGIM
                     LREVRVVADRESEIAAAVNELRRRYTYVFTTGGIGPTHDDVTTDAVAMAFGVDVIVEP
                     EAVAAMRKRFSDEELTPARLRMARVPEGAALIDNAISRAPGFMLENVIVMAGVPRIMQ
                     VMLDAVAPRLAKGKPIMTRAVRLDVPEGDAAPGLADLQASHPEVQIGSYPFFENKRLG
                     TYVVLRSTDQDRLAAAVTDLWIFIDRQGFRAVATEDK"
     gene            17370..18191
                     /gene="map"
                     /locus_tag="VFY74_04045"
     CDS             17370..18191
                     /gene="map"
                     /locus_tag="VFY74_04045"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5930914.1"
                     /translation="MSNLEFARAQARDTKIKLHGADDFAGMAKAGRLVAGVLDLLVDE
                     VKPGVTTEALDRFVFEFAMDHGAVPAPLNYRGFPKSICTSLNHVVCHGIPGPRTLKDG
                     DILNIDVTLIVDGWHGDSSRMYAVGKIPRAAERLLEVTYDALMRGVAAVRPGATTGDI
                     GAAIQTYAEGERCSVVRDFCGHGLGRVFHDRPNILHYGEPGEGLVLKPGMLFTIEPMI
                     NLGKPHVKILSDGWTAVTRDRSLSAQFEHTVGVTETGCQVFTTSPRGLHRPPYRH"
     gene            18207..18897
                     /gene="radC"
                     /locus_tag="VFY74_04050"
                     /pseudo
     CDS             18207..18897
                     /gene="radC"
                     /locus_tag="VFY74_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            complement(18932..19927)
                     /locus_tag="VFY74_04055"
     CDS             complement(18932..19927)
                     /locus_tag="VFY74_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5930915.1"
                     /translation="MKRLKPLAILVLLVPLMIVARSVNAETGATPPIKVQALNDYLIY
                     FFDGRRPAERYAKDWNWFDDAAMKLGVGTYAIHSGDEAIVYDTFTSVPQAKFVRDYLE
                     KMGIRKFTVVHSHWHLDHVAGDAAYDDSDVIATTLTRDALQKQKADIESGKLWGPPPI
                     TPLRIPNVTFDDTKQLKVGDIDLELRRINIHSVDGCVIYIPKDKILLAGDTLEDSLTY
                     MIEVEKLAEHVKNLKDMRQWDVAKIFPNHGDPEVIMKGGYDKTLIDATASYVTKMLSK
                     SHDPDYLQGTMEDYIGDAAGKGWVHIFEPYRDVHTQNLKLVHDYWKDKPLPEIAP"
BASE COUNT         3778 a         6454 c         6496 g         3397 t
ORIGIN      
        1 cgaaataggc gtcgatcgat tttaccacgg cttcggcggt gcggtcgcgc caggcctcgg
       61 aggtgagcag cttctcgtcg tcgggattgc tcaagaaccc gagctcgagc agcaccgagg
      121 gcacgtcggg agcctttaag acgcggaatc cggccgatct tattttgcgg gtgtgcagcg
      181 ccccccgcgc ggcgagctct ccgacgatcg accgcgcaaa caccgcggag cggttctgcg
      241 tttcgcgttg cgccaagtcg atcaggatat tcgcaagaac ctcgtcggag tcgctcggca
      301 gctcgatgcc ggcgagcgcg tcggaaaagt tctccttggc ggcaagcgcg cgggcctctt
      361 cgtcggaggc cttctccgac agcgtataga ctgtggcccc cgtcgtcttg tcgatctctt
      421 gggggaagta gtcggcatgg atcgagagaa agaggccggc ccctttcttc tgcgcgaatt
      481 ccacgcgctc gcgcagcgcg aggaacctat cgtcctcgcg tgtgagatag acctcgtagc
      541 gaccggcagc ttcgagtttg gccttcaacg cgcgcgcaaa ggtgaggacg acctcctttt
      601 cggtgatgcc cgaggcgctc ttggtgccgg gatcgaggcc gccatggccg gcgtccaaca
      661 ccaccacggg cttggcgtag ggaggccttg agggcgtttg cggggcgacg atcggcatct
      721 ccttttccgc ctgcgctctt gcttccttct gcttggccag aaatgtcttc tcgtccgtcg
      781 gcacgagatc gacgacgaga cgcgcgggct gatcgtcgcg cggttcgagg acgaaggctt
      841 tgtcgatgag gaacggtccg gcgacatcga tgacgatgcg cgccttaccc ggcgcgaaca
      901 gtccgtagcg gtaggcagtg acaagtcccc gcttctgctt cccgatcccg tcgggcatct
      961 ggaagttcac gtcgggagcg tcgacgatga cgcggtaggg attgccgagc gcgaagacgt
     1021 gaacgtcgac cttcctggac aggtctgcga tgaagcgcgt gcgttcccga tcgcccgcaa
     1081 cgcgcgcgtc gttggcgacg gccggggcgg tttcagtcgc gcccgcgaac gacgcggaga
     1141 tcagcgccaa ttcaaggccg acgaaggcca gcatcggcac ggccgcaagc aaggcacgcc
     1201 gaacgtgcat cgtcccccac agtctaagcc ggccaacgaa tcgccccgcc gctccgcagg
     1261 gcgcgtgacc ggttttgcgc ctaaataggc tcggaaatcc gccaaaactt aaccacgcag
     1321 tcgttaagcg actgttgtgg cccttttttg gggctcccga cccgcgacgc ttgccaaatc
     1381 tcccgccgta cccgtaataa ggatcaggcc cgcaatgcgg gccagagccc ttggacgcca
     1441 aacggcgata cgggcggaat tgtttcccgc gtcgggcctc gttctgacgg ccaggacgct
     1501 agaaaaggcg gcaaggtgag agcaggccgc caaactctct cgagccgcgt tcaagacgcg
     1561 gcggtgagga ctgcgatgca agatcagtgc aatcctataa gtatcggaaa tgacaagact
     1621 tttacgatac ttccgattgc cttccggcca gtgatggccg cgcccttggg cccgaaagct
     1681 gggccgctcc tggggcgtcg cagcgaatca tggtctattg gtcattggcg cgcgcgaggc
     1741 tcggacgcga agatttcttg gcggacgtct gttccgccct ccgagaccag cgcggcggag
     1801 ataacacaac atggcaagca aaatgctgat cgatgcggcg catccggagg aaacccgggt
     1861 cgtcgtcgta aggggtaatc gcgtagagga attcgactac gaatcagcaa acaagaagca
     1921 actcagaggc aatatttatc ttgccaaagt cacgcgcgta gagccgtcgc tgcaggcggc
     1981 cttcgtcgat tacggcggca atcgccacgg cttcttggcc tttactgaaa ttcatcccga
     2041 ctattaccaa atccctgtcg cggatcgtca ggcgctcctt gaggaagagg ccgccgagga
     2101 aagccgcgcg tccgcggcgg aggccgcgga ggacgaggcc gcggccggac gccgccacgg
     2161 gcgccggcgg cgcggccgcg ggcgtcatga gggacaaggg cgccgcgacg ccccctcgcc
     2221 gagcgccgct ccggcggaac cgatgccgat tgcgaaattg gaacatgacg acgacgacga
     2281 gatcgatacc acgccgcgcg aggatccgca ggtcgtcgcc atggaggcga ccgatctcat
     2341 ccacaaagtg tccgagcccg ccgaggagcc cgacgcggaa accgaagaac gccatttcgc
     2401 cgcagagccg gagcccggcg atgccgggga agacgaggat gaggccgagc cgggcgagat
     2461 cgtcggccaa ccggtggcgt tgaaagaggg caatggcgag accgccgtcg aggcggtcga
     2521 atcggtcggc tctgaggacg tgctcgagga agtgcccgag cgggcaagac gccgccgcgg
     2581 gcgcccctac aagatccaag aggtaatccg ccgccgccag atcatcctgg tgcaggtggt
     2641 gaaggaggag cgcggcaata agggcgcggc tctgaccact tacctctcgc ttgccggccg
     2701 ctacaccgtg ctcatgccga atacggcgcg cggcggcgga atctcgcgca agatcaccaa
     2761 tcccaccgac cgcaagcgct tgcgcgagat cgcaggcgag ctcgaagtgc cggaaggcat
     2821 gggactgatc atccgcaccg ccggcgccca gcgcaccaag accgagatca agcgcgatta
     2881 cgactatctc ttgcggctat gggagaacgt gcgcgatctc accctgaaat cgacggcgcc
     2941 ggcgctcgtc tatgaggaag ggagcctgat caagcgctcc attcgcgacc tctacaacaa
     3001 ggacatcgac gaggtgctgg tggcgggcga cgacgcctat cgcgaagcca aggacttcat
     3061 gcgcatgctg atgccgagcc acgcgaagaa cgtgaagccg tacaaggagc ccgagcctgt
     3121 cttcatcgga taccaggtcg agcgccagct tgccgcgatg ttctcgccgc aggtcgtgct
     3181 gaaatccggc ggctatatcg tgataaatca gaccgaggcg ctggtttcga tcgacgtgaa
     3241 ttcgggcaag gcgacgcgcg agcacaatat cgaggacacc gcgctcaaga ccaatctgga
     3301 ggccgcggag gagatcgccc ggcagctgcg cttgcgcgac ctcgccggac tgatcgtgat
     3361 cgacttcatc gacatggacg agcgccgcaa caaccggctg gtggagcggc gcctcaagga
     3421 agcgttgcgt cacgatcgcg cccgcatcca ggtcggtcgc atcagccatt tcggcttgct
     3481 cgaaatgtcg cggcagcgcc tcaggaccgg catgctcgaa ggctcgacca agccttgccc
     3541 catgtgtcag ggaaccggca tggtgcgatc cgtcgagtcg gtcgcactcg atattctgcg
     3601 ctccatcgag gaccggctca taaccgacgg cgtggtgccg ctcgtcgcca ccaccgcggt
     3661 cgacgtcgcg ctctatatcc tcaatcagaa gcgcatccat ctgaacgaca ttcagcaacg
     3721 ctatcgcgtt gccatcacgg tgaatgcgga tgaggacatg cacatctcgc aattcgtgat
     3781 cgagcgggcg gcggaaggcg agatcgccaa cggctcggtc ccggtgcata tggattgggc
     3841 ccatcaccaa gaaacacccg ctcgcccggc ggcggcagaa gcggaagccg aaccgccgcg
     3901 atcccgttcg cgacggcggc ggcgacggcg gcgttcggac ggcggcgagt tccatcggcc
     3961 gatgcactcg gccgctgcgg agcccgagga ggagatggag gagagagccg agcagccgcg
     4021 gaccactggc gccgcggcag cgagcggcgg cgaagcgaaa gaggaaggcg gtgcgcaagc
     4081 ccgcaagggg ccgcgccgca gaggccgccg cggcggacgc cgcggccgtt ccggccagcg
     4141 tccccaggga gccccgggag aggagggacc gagcgacagc cggatcggcg ttcgcggcga
     4201 gagagccgca acctctcccg tcgacgcaag caagaacggg atcggtggcc cgcgatctga
     4261 tgaggaggca tctgcgcaag agcccgctcc cgtccttgag actgtcacaa cggccagcga
     4321 gcgcgaaccg ccaagtggtg cacatgcgga aactgggggc gagccggaac cgccccgccg
     4381 ccgcggctgg tggcagcgct agggaggtcg gggagcgcgg agggcggccg cgcttctgcc
     4441 gataaaggga ggggcttcgc ccgtcacgtt gtcgccgtat tcaaccgcca agcaaagacg
     4501 gcggcgaaga gacggcccaa ggcgacagca tatcgccagt actctaaagc atagagccgg
     4561 cggctccgcc gcggccctcg tcgagccaag cgaacaggcc gaacatccat gccgctagag
     4621 gctcatgtcc aaccccggtc aaaagcgcgg cggctccgcg cggtgtttgt cggggcgctt
     4681 tccgccgctc aaatcgtcgc gacgacggcg gatgcgaagg ggctcatccg tgacgccgag
     4741 atcgaagcgc tgatccgcat ttacgccaag cccatcctga acgcggccgg catcggctct
     4801 caaggcatcc gcattcacct cgtcaacgat ctgagcttca acgcctttgt cgtcgacggg
     4861 cacaacatct tcatgcatgc cgggggcctg acgagcgcca agacgcccaa tcaggtgatc
     4921 ggcgtcctcg cccatgagac cggacatatc accggcggtc atctcgcccg gctcaggacc
     4981 caggtctcgc gcgcgaaatc ggcggcgctc atgatgcaaa tcatcgggct tgctgccatg
     5041 gtcgggggcg ctttcgcagg agccccgggg atgggccagg ccggtatggg ggtggccaca
     5101 ggcggaatgg acgccgcctt tcgcacactg ctcgcctacc gccagaacga ggagtcgtcg
     5161 gccgaccagg ccgcggtcac gttcctcaac gccaccaagc aatcgggccg cggaatgatc
     5221 gagactttcg agttcatggg cagcaagctt gtcggggtcc aaggcatcaa cccttacctg
     5281 caatcgcacc cgttgccgca gcagcgcatc acgaatctca gggcgcttgt cgaatcgagc
     5341 ccctactaca acaacgtcga cccccccgag ctccagttcc gtcacgactt gatgaaggcg
     5401 aagctgttcg gctttctcga cgacccggcg actgtgttca accgctaccc gcagaccgac
     5461 cagagtcttc cggcgctcta cgcgcgagcc atcgccgtct accggaaatc cggcatgaag
     5521 gctgcggcgc cgctcatcga tcagctgatc gccgccaaac ccgattggcc ctatttctac
     5581 gagctcaaag gccagttttt gttcgagagc ggcaacccgg cggcggcggt tcctccgctt
     5641 cgcgaggcgg tacggctcag tcccaacgag ccgctgcttc gggtgatgct gggccaggcg
     5701 ctccttggcg ccaacgaccg caagctcgtc gacgaggcca tcaccaatct gcgaaccgcg
     5761 cttacacgcg aggacaactc ggccatgggg taccgccagc tcgccgcggc ttacgcccgg
     5821 aaagccgagg cggcacaagc agttggcggc aagaagcagt tcatggcgca agccgcgctt
     5881 gcatcggccg aggcgtattt ctatgaaggt cagctgaagc tcgccaaaca acaggccaag
     5941 cgcgccaggg acggcttcat cgacgggacg ccgaattgga tcaaggccga cgacatcctg
     6001 gctttcgaag tcccccaaac caactgacat aaagcgcctg gagatttcag catgaccccc
     6061 aagacaggct tggccacggc gctcgtggcc gcaatcgtgg ccgcgctcgt gaccgcggcc
     6121 accctcctcg tcgtctggcg cgcggcgccc ggcgtggtcg aggttcccgt ggtcaaggtt
     6181 ccccgggcgg cgctcgacga gaagaccatc atcgatgtcg tccgcgacta tctcaccaag
     6241 aaccccgaga tcttggtcga gatgacgacc gagctcgaca agcgccagca ggccgcggag
     6301 tcggcgaagc agcaaaaagt gatcggcgaa aatgccgaca ccatcttccg ctcgccgctt
     6361 tcctatgccg cgggcaacgc caacggcgat gtgaccgtgg tcgagttctt cgattacaat
     6421 tgcggctact gccgccgggc gctgcccgac gtggtcaagc tcattgatac cgacgacaag
     6481 gtgcgcgtcg tgttcaagga gctgcccatt ttcggcgagg agtccgagtg ggcggcgaag
     6541 gccgctctcg ccgccggcaa gcagggaaaa tatttcgaga tgcaccagaa gctctacgcg
     6601 gcgccgggca agtccgacaa agagaaggcg ctccgcgtcg ccgccgaaat cggcctcgac
     6661 gtgccgcagc tcgagaagga catggaagat ccctcgatca aacaagcact cgaagagacc
     6721 aaggagctcg ccacgaaact cggcttgcag ggaacgccgc tctacctgat cggcgatcgg
     6781 atgatccctg gcgctcctga cgacctttac gatcagttgg tggcaaaggt cgccgagatc
     6841 cgggaaaagg gctgcaaggt gacctgctga gcgcctcgtt aaggagacgt tcgccctcgg
     6901 gcgctccact cattttgccg aatggccgaa aagctggtaa aggggccggt ccctcatgcc
     6961 ggggccagcg caggggagcc cgcgcaccgc ccggccgtat caggcgtttc ctagccgcca
     7021 tggcgaagcc gatctacgtg ttgaacggtc ccaatctcaa cctgcttggg acccgcgagc
     7081 cagaggttta cggccgggag acgctcgatg acctgcgcgc ccagtgcgag cgcaaggcgg
     7141 aggcgcttgg attttcgctg gtgttccgcc agagcaatca cgagggcgag ttggtggcat
     7201 gggtccaaga ggcgagaacg gaggcggccg ggctgatcgt caatgccggg gccttcaccc
     7261 acacctcgat cgcgctcctc gatgcgttgc tggcttgcac ggtgccggcg gtggaggttc
     7321 acctgtcgaa tatcttcgcc cgcgaaccgt ttcgacacca ttcatacatt tccagggcgg
     7381 taaaaggcgt gatctgcggc ttcggggcga ccggttacga gcttgccatc gaggcgctga
     7441 cggcaacgct tgcgccggcg aagccaaagt gaccggggcg gtaaaaggaa gccacccatg
     7501 agtaaacaag ggggcagctt cgacaaggac ctgatccgcg agcttgccgc gcttctggag
     7561 gagaccggtc tcaccgagat cgaggtcgaa catgacgggc gacggatcag agtcgcgcgc
     7621 ggggtgaacc tcacgaccac gctgaccacc gaccttccgc aggccgaacc gcgcgagagc
     7681 gcccgcgcaa aaggcgacgg tccaaagccc gcgaaacggg cggcgggcca aggcgaggag
     7741 ttcatcgtac atcccggcac cgtgacatcg ccgatggtgg gcaccgccta tcgcgcgccc
     7801 gagcccggag cgccgccctt catcgaaatc ggatcgaagg tcacggcggg gcaaacgctc
     7861 ctcattatcg aggccatgaa gaccatgaac cagatcccct ccccccgcgc cggcaccgtc
     7921 atcgccatcc tggtcgagga cggacagccg gtcgaatatg gcgagccgct ggcggtgatc
     7981 gagcaacgct agccggggcg cgatgttcga caagatcctg atcgccaacc ggggagaaat
     8041 cgcgcttcgc gtcgaacgcg cctgcaagga gttgggcgtc gccaccgtgg cggtgcactc
     8101 gaccgccgac gccgatgcca tgcatgtgcg cctcgccgac gagagcgtgt gcatcggcgc
     8161 tcccccggcc aaggacagtt acctcaacat tccggccatc gttgccgcct gcgagatcac
     8221 gggcgccgac gcggtgcatc ccggctacgg cttcctggcc gaaaatgctc gcttcgccga
     8281 gattctcgag gagcacgaga tcaccttcat cggcccgacc gccgaacacg ttcagctcat
     8341 gggagacaag atccaggcca aaatcgcggc gcaacagatc ggcatcccgg tggtgccggg
     8401 ctcaggcccg gtcgatgact tcgatgaggc gaagcgtgcc gcgcgcgaga tcggctatcc
     8461 ggtgctggtc aaggcggcag cgggcggcgg cggacgcggc atgaagcttg ccctgtcgga
     8521 ggacgagctt gagcctgcgc ttctgaccgc gcgcaacgaa gcgaaggccg ccttcggcga
     8581 cagtaccgtc tatctcgaaa aatacctgac ccgtccccgc cacatcgaag tgcaggtcct
     8641 gggcgacgga agggggaatg ccatccatct cggcgagcgc gactgttcgc tgcagcgccg
     8701 ccatcaaaaa ctctgggagg aggcgccctc gcctgcgctg aacgaaaacg agcgcgtgcg
     8761 gatcggcgag atcgtagcca aagcgatgca agcgttaagc tatcgcggag tcggcaccgt
     8821 cgaattcctg tacgaggccg gcgcgttcca tttcatcgag atgaacacgc gcctccaggt
     8881 cgaacatacc gtcaccgaga tgatcaccgg gatcgatctc gtcatcgagc agatcaggat
     8941 cgcggcaggc gcgccgcttt cgctcaaaca ggaggacatt cccttcaacg gccatgccat
     9001 cgaatgccgc atcaacgccg agaacccgca gaccttcagg ccgtcgccgg ggacgatcac
     9061 gcatttccac acgccgggag ggctcggtgt cagggtcgat tcgggcgctt acgcgggcta
     9121 cactatcccg ccttactacg acagcctgat cggcaagctg atcgtgcacg caaggacccg
     9181 caacgagtgc ctgatgcggc tcaagcgggc acttggagaa ttcgtggttg atggcatcga
     9241 gaccaccata ccgttgttta cctcgcttat cgccgagccc gatatcgtca atggcaacta
     9301 cgatatccat tggcttgaga aacacctcgc caagcacggc acccgcggaa agcgctgatg
     9361 acggcgattg acgacgtcac catcgagatc acgccgcagg tgctgctcaa ggcctatgcg
     9421 tgcgggatct ttcccatggc cgagagcgcg gaggacaacg cgctctattg gatcgagccg
     9481 gagcggcgcg gcattcttcc gctcgaccgc atgcatgtgc cgaagcggct tgcccgcttg
     9541 atccgcagcg gcggcttcga ggtcaaggtc gaccgcaatt tcgaggccgt catcgacggg
     9601 tgtgccgcgc cccgctccgg ccggcgctcg acctggatca acggccgcat caggagcctt
     9661 taccgcgagc tgtttgcgct cggtcattgc cacacgatcg aagtctggca agacggcgtc
     9721 ctcaccggag ggctttacgg ggtgcatctc ggccgcgcgt tcttcggcga gagcatgttt
     9781 tcgagggcgc gcgatgcgag caaggtcgcg ctcgtctatc tcgtggcacg attgatctac
     9841 ggaggcttta cccttctcga cacgcaattc gtcaccgggc accttgcgcg cttcggcgcg
     9901 atcgaggtca gccgggacga gtttcagcga ttgctggagt ccgcgttggg cggtggcttg
     9961 gcccacggca gcggctcggc cggctcgggc ggcggtggcg ccgcagccgc gtcggcgggc
    10021 gcctcccccg gcggggcctc ggcaggaggt gcgccgggag gtgcctcggc ggccggcgcg
    10081 gcgccagggt ctgcggcgtt cgcggcgttg ccggaaggcg ccggcggcgc attcgtcttg
    10141 cagttggtca gccagacgtc gaagaccgga tgctcgaccg cgtgaaggcc ggggctttcg
    10201 gcgagcatcc agccggtgaa gatgcgctgc acctcgccgg tcagcttgac ctcatcgacc
    10261 tcgacaaaag acgcggtgtg gggctgctcg gtcggcgggc gcgtgtcgca aacccgcggc
    10321 gtcactttga gggcgccaaa ctcgaccgtg ttgttgatgg gaatctcgag acgggagatg
    10381 cgccccgtga ccttatcgag ggcggcgaag accgcgatcg ggttcctgac gatctcggcg
    10441 ccggccgggc ccggcgcgaa gccgaaggcg agagcgaagg agagcaagga gaggggcagc
    10501 caggaggctg gccggcgcgt catcccgatg ctcgctcgtt ccgccatgcc gcacacctcg
    10561 tgaacgggcc gtttgattgt cgctccgtct tggcttcggc cgcgagcatt actttctcgc
    10621 aaagcccacc acgtcgcatc atgacccgcg gaaaacgcct cgtccaggat tggggcatcg
    10681 atgtggcggg cccgctcagt cacggcgcac ggcatggaag cgaagccgaa tatggtcggc
    10741 ggtccagcgc ccttccgtgt cgcagagcga gggccgcaac agttcgacga cctcccccac
    10801 gacctcgccg cgcagaggct cgggaaattg gtcgaagaag gagcggccga aagtcctgag
    10861 ccatccctcc atgccggtct tgagcagcgt cggacgcggg acgagcacga tctctttgac
    10921 gctgaagccg ccttcggcga ggaggcgggt atattcttcc ggcgtgggaa agaaccaggg
    10981 cgcggtgacg gacggatcgc cgccgcggag cgcaccgacc gcgcggatgg cagtcgcgat
    11041 cgccgcgaca ttgccgtggc cgccaagctc gcccacgaag cggccgccgg ggcgaagggc
    11101 gcgcgcgaca ccttcgatca ccaatttcgg ctttcgcatc caatgcagcg ccgcgttgga
    11161 gaagaccgcg tcgaactcgg atacaaaatc caacgcgtgg ccatcgagct tgcggacagg
    11221 aagccccttc atgcgggcga cggccagcaa ctcctcgctt agatcgacgc cgaggacatc
    11281 ggcgccaacc gccctgatct ccgccgtcag tgcgccgtcg ccgcagccga gatcgaggat
    11341 gcgctcgccg gccttcgcgg cgagcatgcc gaagacaggg ccggcgagct cgtgcacgaa
    11401 gcggccgttc ttggcatagt cggacgccga ccagcgctgc accgccttgg gcggcacggc
    11461 atcgagcgcc gaaggctctg tcagttgggc tgccacggct cgtagtccag cggaggtttc
    11521 gcggcccccg gacgcagcgt cgagcccggc ggccgatagg cctcgtaggt tccggtcagg
    11581 ttcgggcgat gcgcctgttc ccagggccgc ggggtgtagt cttcctcggt tggcggcaca
    11641 tcgacggtat gatgcagcca ggcgtgccat tccggcggga cctgcgaggg ctcggcgagg
    11701 gtgcgataga tcacccagcg gcgcttcccg ccccgttccc ggtagtagac gttgcccagt
    11761 ccatcctccc ccacgcgctc ccctttccgc cacgtataga ggcgcgtgcc gatggtgttg
    11821 cccgtccacc acgcgaaaag ctcggtgaca aatcccatta ttgctttcgc cgcttcgttt
    11881 gcgcggggac tatggcctca aggcgccgcg cctgtccagc cggcccgtcc ggcgcgattc
    11941 gtggtgggat gatcggatcg gtcgcgcccg cgccatgacc gactcgccac atgccattat
    12001 ttaagtaagc gctatgtccg ccgccggccg gcgagatgtg agatccatgg agcgaaagcg
    12061 cctcccgagg cccgccttgt tattgtccct cgctgtccac gtaacgtccc agactccccc
    12121 acagccttta gtggtatagt aggaagtcca tacatatata ttgatggatt tgacgaatcg
    12181 ccccagtttc agcggttacg cgccaaatcg gcaaagagcg cgaagggggt acgacaggat
    12241 gcggatcgag agacgtttca ccgaggcggg caaatcgccc tatgacgcca tcccgttccg
    12301 ggagagcgga agcgagatcc gcaatcccga cgggtcggtc gtcttttcgc tcgcgcggtt
    12361 ccaggtcccc gaacgttgga gccaggtcgc tgccgacatc ctggcgcaga aatatttccg
    12421 caaggcgggc gtgccggcga aggcgaagcg ggtcgaggaa acttcggttc cctcctggct
    12481 gtggcgctcg gtccccgatg aggccgcgct cgcagcgctc cccgaggccg agcgctatgg
    12541 cggcgagacc gacgcgcggc aggtgttcga ccggctcgcc ggcacctgga cctattgggg
    12601 ctggaagggc ggctacttca ccagcgagga ggacggtcaa gccttcttcg acgagctccg
    12661 ctacatgctc gccatgcaga tgggcgcccc caacagcccg caatggttca ataccggcct
    12721 ctactgggcc tacggcatcg atggcccgag ccaaggtcat tattatgtgg atttcgaaac
    12781 cggcgaggtc aaagcctcga cctcggccta tgagcgcccc cagcctcatg cctgcttcat
    12841 ccagtcgatc gacgacgacc tcgtcaacga gggcggcatt atggatttgt gggtccgcga
    12901 ggcccggctg ttcaaatacg gctcgggcac cggcaccaat ttctcgcggc tcagaggcga
    12961 gaacgagccg ctctcgggcg gcggcaagtc ctccggcctg atgagcttcc tcaagatcgg
    13021 cgaccgggcg gcgggcgcca tcaaatcggg cggcaccacg cgccgcgcgg ccaagatggt
    13081 cgtggtcgat atcgatcacc ccgatatcga ggactacatc aactggaagg tcaccgagga
    13141 gcagaaggtg gcggcgctcg tcaccggctc gaagatcctg tcggagcggc tcaacgccat
    13201 cctcaaggct tgcgtcaatt gcgagggcga cggcggcgac tgcttcgacc cgaaccgcaa
    13261 cccggcgctg aagcgcgagg tgagagccgc ccgcaaggcc aaggtcccgg acaacgccat
    13321 ccgccgggtg atccagttcg cccgccaggg ctataccgcg atccaattcc cggcctatga
    13381 cacggactgg gattcggagg cctatcgaac cgtgtcgggc cagaactcca acaactcagt
    13441 gcgggtgaca gacgatttct tgcgcgcggt cgaggccgac ggcgactggg acctgactta
    13501 tcgcaggaac ggcgccgtgg ccaagacggt gaaggcgcgg gcgctgttcg agcagatcgg
    13561 ctacgccgcc tgggcctgcg ccgatcccgg catccagttc cacaccacca tcaacgattg
    13621 gcacacgtgc ccggaggccg ggccgatcaa ggcgtcgaac ccctgctcgg agtacatgtt
    13681 cctcgacgat acggcctgca acctcgcctc gctcaatctg atggcgttcc gaacagagga
    13741 cggcagcttc gacatcgagg cctatgagca cgcgatcagg ctttggaccg tcgtgctcga
    13801 gatcgcggtg gtcatggcgc aattcccgtc gcggcggatc gcgcaattgt cttacgacta
    13861 ccgcacgctc gggcttggct acgccaatat cggcggcctg ttgatggcgg caggcgttcc
    13921 ctacgactcg ccgcaagggc gcgcgctctg cggcgcgctc agcgccatca tgacggggat
    13981 ctcctatgcc acgagcgcgg agatggcgcg cgagcttggg cccttcccgg gttacgcggc
    14041 aaaccgcgaa gccatgcttc gggtcatgcg caatcacgcc cgggccgctg ccggcaagcc
    14101 tgcgggctat gagggcctcg acacggcgcc cgtgcctctc gaccacgcct cctgtcccga
    14161 tccgcggctg ctcgcccatg ccacgcatgc ctgggagaag gcgatcgagc tcggccgcaa
    14221 gcacggctac cgcaacgcgc aagcgacggt gatcgccccg accggaacca tcggtctcgt
    14281 catggactgc gataccaccg ggatcgagcc cgatttcgcg cttgtgaagt tcaagaagct
    14341 cgccggcggc ggctatttca agatcatcaa ccgcatggtg cccgaggcgc ttcgggggct
    14401 cggctatccg gacgaggcga tcaagcgcat cgtcgaatat gccgtgggtc atggcacgct
    14461 cgcaggcgcc gctggcgtca accacgaggc gcttgccgcc aaggggttca cgcccgagaa
    14521 gatcgccgcg gtcgagaagg cggtggcgtc ggccttcgac atcaaattcg tgttcaatcg
    14581 ctggacgctc ggagaggatt tcctgaccgg cacgctcggc ctctctcccg agcggatcga
    14641 ccagcccgat ttcgatctcc tggcggagat cggcttcggc aagcgcgaga tcgaggccgc
    14701 caacgaatat gcctgcgggg ccatgacgct ggaaggcgcg ccggggctca accccgagca
    14761 cctgccggtg ttcgattgcg cctcgccatg cgggcgcaag ggcaagcgct tcctgtcggt
    14821 cgagagccac atccgcatga tggcggcggc gcagcccttc atctccggcg ccatctccaa
    14881 gaccatcaac atgccgaacg acgccacggt cgaggactgc aaggaggcct atctcctctc
    14941 gtggaggctc gcgctcaagg ccaatgcgct ctatcgcgac ggctccaagc tgtcacagcc
    15001 gctcaacgcg cagctcctcg ccgacgagga ggacgagcag gaagaggtgc tggccgcagt
    15061 cgtgtcggat cagccgcagg tgcagcgtgc cgcggtcatc gccgagcgca tcgtcgagcg
    15121 cgtggtcgag cgcatgcggg cgcgcgccga acgcgaacgc ttgccagatc gtcgcaaggg
    15181 ttatacgcaa aaggcggtcg tcggcggtca taaggtctat ttaaggactg gagagtatgc
    15241 agacggtcgt ctgggcgaga tctttatcga catgcacaag gaaggcgccg ccttccgctc
    15301 gctcatgaac aactttgcga tcgcagtgtc cgtcggtttg cagtatggcg ttccgttgga
    15361 agagttcgtc gatgccttta ccttcacgcg cttcgagcca gcaggcccgg tgcgcggcaa
    15421 cgaggcgatc aagaacgcaa cttcgatcct tgactatttg ttcagagagc ttgcagtttc
    15481 ttacttgggg cgctacgagc ttgctcatgt tgatccaagt gagatcggca gcacaacact
    15541 cggctccggt gacgcgggcg aaggcaagcg ttccaccgaa gctctcgtct cgccaggcta
    15601 cacgcgtggt cgcctcagcg agcggaagct tcatgcgccg agcaaagcgc agagcactgg
    15661 tgcgcatgcg cgtgcgttgc aggcggacgc ccgcgacgac gcgcagccgc gcaccacaag
    15721 ctttgccgtc ccgtcctctc aactcccttc agccgagacg accgagcaag tcgcccgtgc
    15781 cgtcgaaacg ctcgctgaac gtggcgcaac agaacttgtg gccgaagcgc gcattaaggg
    15841 ttatgaagga gaggcatgtg gagagtgcgg gaacttcacc ctcgtacgca acggcacgtg
    15901 tcttaaatgc gatacgtgtg gagcgacgag cggatgtagc tagagtgaat taacagccag
    15961 ccagcccgaa ggagggatgc actatggcag cagcgaagag aaagacagcg aagaggaagc
    16021 cggcccgcaa gaccgcggcg gcgaaaaagt cgtcggcgaa gaagaccggc agcgcccggc
    16081 gcaagaagag ataggcttgc ttagaggcgc ggggctcgtc cccgcgcctc ctttattttc
    16141 cttccaaatc aaacgattgt tcggcccaag cggcgctccc cagagcgcgg cgcttgcggg
    16201 ctttttgccg cgacgcctgc cgccgtttct cgcctcccgc atcgatgcac cagcgctccg
    16261 cttgcccgtg cacacgcacg tcgcgttgcg cgatgtgaac gaaaacgaac gatgagcggg
    16321 agcagacatg cggctgtttc gaccgcgccc gcgcgcgggc atttccgcgt tgcgccatga
    16381 gccggccgcc gccggcgggc cgacccggtc aagaggcggg gtgattcgtt ttcgtcaagc
    16441 cgagctcatt tgtcttcggt ggcaacggcc cgaaatcctt gccggtcaat aaatatccac
    16501 agatctgtaa cggccgcggc cagccggtct tgatcggtcg agcgcagcac cacatacgtg
    16561 ccgagacgct tgttctcgaa gaagggatag ctgccgatct gcacctcggg atgggacgcc
    16621 tggaggtcgg cgaggccggg agcggcgtcg ccctcgggca cgtcgaggcg gaccgcgcgg
    16681 gtcataatcg gtttcccctt ggcaagacgc ggagccacgg cgtcgagcat gacctgcatg
    16741 atgcggggga cgccggccat gacgatgacg ttctccagca tgaagccggg cgcgcgggag
    16801 atggcattgt cgatcagcgc tgcgccttcg gggacgcggg ccatgcgcag gcgcgccggc
    16861 gtgagttcct cgtcgctgaa ccgcttgcgc atggcggcca cggcctcggg ctcgacgatg
    16921 acgtcaacgc caaaggccat ggcgacggcg tcggtggtca cgtcgtcatg ggtgggtccg
    16981 atcccgccag tggtgaacac gtaggtatac cgccggcgaa gctcgttcac cgcggcggcg
    17041 atctcgctct cgcgatcggc cacgaccctg acctcgcgca gcatgatgcc gatgcgggtc
    17101 aggtaggcgg cgatatagcc gatattctcg tccttggtgc ggcccgacag gatctcctcg
    17161 ccgatcacga ggagcgcggc cgtgacggtt cgctggttct gctcggaggt catggcgaac
    17221 agaaacgctt gccgaaacga aaagtccagc gcctcgccgt cgggttcatg catcgtgctt
    17281 tccgcgaaga caagaaaacc ggcgagcgcc ccgttgcaca ttgtcatatt tccccctaag
    17341 tgaacctcaa ttccctataa tctcatccca tgtccaatct cgaattcgcc cgcgcccagg
    17401 cgcgcgacac caagatcaag ctgcacggag ccgacgactt tgccggcatg gccaaggcag
    17461 gccggctcgt ggccggcgtt ctcgatctcc tcgtcgacga ggtgaaaccc ggggtgacga
    17521 ccgaagccct cgatcgcttc gtgttcgaat tcgccatgga ccatggcgcc gttcccgccc
    17581 cgctgaatta tcgcggcttt cccaagtcga tctgcacctc tctcaaccat gtggtttgtc
    17641 acggcatccc gggcccgcgc acgctcaagg acggcgacat tctcaatatc gacgtgaccc
    17701 ttatcgtcga cggctggcat ggggattcga gccgcatgta cgccgtgggc aagatccccc
    17761 gtgccgccga gcgcctgctc gaggtgacct acgatgcgct gatgcggggg gtggccgcgg
    17821 tgcggccggg cgccaccacc ggcgatatcg gcgccgccat ccagacctat gccgaaggcg
    17881 agcgctgcag cgtggtgcgc gacttctgcg gccacggcct ggggcgcgtt ttccacgacc
    17941 ggccgaacat cctgcactat ggcgagccgg gcgaagggct cgtcctcaag cccggcatgt
    18001 tgttcaccat cgagccgatg atcaatctcg gcaaaccgca tgtgaagatc ttgtcggacg
    18061 gctggacggc ggtgacccgt gaccggtcgc tgtcagcgca gttcgagcac accgtcgggg
    18121 tgaccgagac gggatgccaa gtgttcacaa catcgccgcg agggttgcat cgtcctcctt
    18181 acagacactg atcgggccaa ggctgaatgg gcttcaagga tgcgggcaag ccacattatc
    18241 tcggccatcg cgagaggctc aggcgccgct tccgcgaggg gggctccgac gccctgcccg
    18301 attacgagct gctcgaactg atcctgttcc gcgccgcgcc gaggcgcgac accaagccga
    18361 tggccaaagc gctgatctca cgcttcggta cctttgccga ggcggtgaac gcgcctgaag
    18421 agctgttgcg cgagtgtccg ggatcggcga ggcggccgtt gtcgagctca agctcgtgcg
    18481 cgcggcggcg ctgcgcctga tgcgcggcga aggctggaac ggcccgtgct gtcctcgtgg
    18541 gcgctcgtgc tcgactattg ccgggcgacc atggggttcg agaacaagga gcaattccgc
    18601 attttgttcc tcgacaagcg caaccagatc atcgccgacg aagtgcagca gaccggcacg
    18661 gtcgatcaca ccccggtcta tgtgcgcgag gtggtcaagc gggcgctgga gctgtcggcg
    18721 acggcgatcg tgctcgtgca caaccacccc tcgggcgatc cgacgccgtc gcgggccgat
    18781 atcgagatgg cgaagcagat cgtgtccgcc gggaagccgc tgggggtcct ggtgcacgac
    18841 catatcatcg tcggcaagag gggccatgcc agcttccgcg ggcttggcct gatctagcaa
    18901 cccctcgccg gcctcgtttg ccgcgccggc gttacggcgc gatctccggc agcggcttgt
    18961 ccttccaata gtcgtgcaca agcttgaggt tttgcgtgtg cacgtcgcga tacggctcga
    19021 agatatgaac ccagcccttc cccgccgcat cgccgatata gtcctccatg gtgccctgca
    19081 aatagtccgg atcatgggac ttgctcagca tcttcgtgac gtaggacgcg gtcgcatcga
    19141 tcagggtctt gtcatatcct cccttcatga tgacctccgg gtcgccgtga ttgggaaaga
    19201 tcttggccac atcccactgc cgcatgtcct tgaggttctt gacatgctcg gcgagttttt
    19261 ccacctcgat catgtaggtc aacgagtcct cgagcgtgtc ccccgcgagc aggatcttgt
    19321 ccttcgggat gtagatgacg cagccgtcga cgctatggat gttgatccgc cgaagctcaa
    19381 ggtcgatatc tcctactttg agttgcttcg tgtcgtcgaa ggtgacgttc gggattcgga
    19441 gcggcgtgat gggcggcggt ccccatagct ttcccgactc gatatcggcc ttctgcttct
    19501 ggagggcgtc acgggtcaat gtcgtggcga tgacgtcgct atcgtcgtaa gcggcgtcgc
    19561 cggcaacgtg gtcgaggtgc caatgactgt gcaccacggt gaactttctg atccccatct
    19621 tctccaggta gtcgcggacg aacttcgcct gcggcacgct ggtgaaggta tcgtagacga
    19681 tggcctcgtc gccgctgtgg atcgcgtacg tgccgacgcc aagtttcatg gccgcgtcgt
    19741 caaaccaatt ccagtccttg gcgtaacgct cggccggacg ccgtccgtcg aagaaataga
    19801 tcagataatc gttcagcgcc tgcactttga tcggtggcgt tgcaccggtc tccgcgttga
    19861 cactgcgggc aacgatcatg aggggcacca acagaacgag aattgccaaa ggctttaggc
    19921 gtttcatatc accctccgcg acttacatcc ctcccaattc ccccctactt tttcccccta
    19981 gaacgatcct accacgccgg agcgcccatg actttgtgcc gcccggccgc aggaccaggc
    20041 ctcctgacca atcctcaata aacagcgtcg atcctgcttt ccggggtgcc gatcttgtcg
    20101 gcgtaggtgg gatcgccaag tgtcg
//
LOCUS       DASUQP010000090         9914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1129279, whole genome shotgun sequence.
ACCESSION   DASUQP010000090 DASUQP010000000
VERSION     DASUQP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129279"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFY74_04060"
     CDS             <1..785
                     /locus_tag="VFY74_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5930916.1"
                     /translation="VRKFSFTGSTPVGKMLYRQCADTVKKLTLELGGNAPLIVFDDAD
                     LDPAVAGAIASKYRNAGQTCVCANRILVQSGIYDRFAKALVDAVKKLAVGSGLEAGTN
                     IGPLINGEAIAKVETLVGDAVKKGAKVLLGGKPDAAGPLFYRPSVVGDVTPEMSIVRE
                     EIFGPVAALVRFDTEAEAIDLANDTPFGLAAYLFSQNLSRAWRVAEKIDAGMVGVNEG
                     IFSNEVVPFGGVKESGLGREGAAEGLDEYLEVKYICLGAIDA"
     gene            933..2024
                     /locus_tag="VFY74_04065"
     CDS             933..2024
                     /locus_tag="VFY74_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5930917.1"
                     /translation="MTAVHAERYSSRRSILKGLGAAAIGITLSSNKKFSALAAEEPKL
                     NLYNWDTYIGETTLEDFKDASGIDVNMSLFATNDELFAKLRAGNPGYDVIVPGSEFVE
                     RMIQAGMLEPLDHAKIPNIKNVAPEFVKDAPFDPGRKYSMPYTWLVIGIGYRKSKVKE
                     VPNSWKWLFDSDVYKGRIAVMSDASELFRNCFKYLGHSLNDASPELLKQAEAMLTKQK
                     PNIKAFHEDNGQDLLLSGEVDVVMEYNGDIAQIMKEDDDVDFVVPKEGAMKQQDTLAI
                     PKGAPHPDNALKFINFILSPEAGAGITKTILYPSPNAAAVALMPASYKDNPVIFPPAD
                     VMAKCEYPNYLGPEFSRLLDEAMTRLRAS"
     gene            2071..2985
                     /locus_tag="VFY74_04070"
     CDS             2071..2985
                     /locus_tag="VFY74_04070"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5930918.1"
                     /translation="MATQTQERRLAFLATALPPIAWVVVFFIAPLAIVWAYSFGQNVS
                     LTEVDVSGTFANYARALEPLYLGIFAKSVFVAGLTTLLCLIIGFPVALSIVFASERWR
                     PWMLLLIMLPFWTNLLIRTYALMSVLRTEGYANFTLEWLWNQGSWLMTLVGLQPLGEF
                     TPLQLLYNNFAVVLGLVYVHLPFMVLPLYAALDRLDKSLIEASLDLGAGHLRTMFSIV
                     IPLAMPGIISGIIITFVPALGAYLTPDLLGGPDSQMIANVIERQFKKANDWPFGAALS
                     FLLMYATFAAIAFRAYFSRGKPAAEHGI"
     gene            2988..3821
                     /locus_tag="VFY74_04075"
     CDS             2988..3821
                     /locus_tag="VFY74_04075"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5930919.1"
                     /translation="MAERRAPLEYNRLWQMRAWLFAVFFFLYAPLVALIAFSFNDSKR
                     TTVWRGFTLRYYEKLFSNDSLLIAFANSLTIAFVATLISLFVGALAAVLLWRYRFPGR
                     LAVEGAFTLPLVVPEICMGVAMLLFFTRVLPWPNDLIWPFNLGSIIIAHVSFCFPFVA
                     LVVGARLASFNKEQEEAARDLGANDWQAFRDVLLPHIKPALIAGALLAFTLSLDDFVI
                     TFFTSGPDTVTFPVKVYSMLRFSVTPEVNAASTMLIVLTIVLMVIMMVMQSKTQKKAK
                     G"
     gene            3827..4978
                     /locus_tag="VFY74_04080"
     CDS             3827..4978
                     /locus_tag="VFY74_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5930920.1"
                     /translation="MDRALAEGTKTAVKPIISFRDISKRWGSTLAVDSVSLDIQPGEF
                     FALLGPSGCGKTTLLRILAGLEVPSEGHVLIDGRDMSEIPPNKRAVNMVFQSYAVFPH
                     MSVADNVAYGLKIDGVPQPERDKRVQEALDLVQLGHLAERMPDQLSGGQRQRVALSRA
                     LVKKPKVLLLDEPLSALDAKLREAMRFELSQLQQKVGITFIMVTHDQDEALAIASRIA
                     VMNKGSIAQLGTPSDLYEFPASRFVADFIGSVNMFEGTLLDDKPTEATVSCPELDPHR
                     VHVPHSVPGKAKAPVWVAIRPEKMTLHKDAAEPPAVPECPAAHNVAKGEIKEISYLGD
                     ISIYHVLLANGRLIRISRPNRSRWDQDNFTWGEKVWVSWAGSSPVVLQN"
     gene            5025..6320
                     /gene="gabT"
                     /locus_tag="VFY74_04085"
     CDS             5025..6320
                     /gene="gabT"
                     /locus_tag="VFY74_04085"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX5930921.1"
                     /translation="MSMSAELHERRKAAVARGVSHASAIYAKRAKNAEIWDEDGNRYI
                     DFGAGIAVVNTGHQHPKIVAAVEEQLKAFSHVCFQVTPYETYVRLAERLNAIAPVAGP
                     AKTMLVSTGAEAVENAVKVARAYTGRPGIVSFSGGFHGRTLMAMALTGKVVPYKKGFG
                     PFPAEIYNVAFPAVYHGVSSEQSLAALNALFKYTADPSSIAAVIIEPVQGEGGFYMAP
                     LDFLRALRALCDEHGILLIADEVQTGFARTGKMFALEHAGITADVVAMAKGLAGGLPL
                     AAITGRADVMDASNPGGLGGTYSGNPLACAAAHAVLEVIEEEDLCGRANAIGKIILDR
                     CRQMKDRSNLNCIGDVRGIGAMCAIELVKDVASGEPAPQLTQTLMKVANQNGLVLLSC
                     GTYGNVSRFLAPLTASDAIVREGMDIFEASLTEAVARAG"
     gene            6350..7672
                     /locus_tag="VFY74_04090"
     CDS             6350..7672
                     /locus_tag="VFY74_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5930922.1"
                     /translation="MNAKLATNDLEAFWMPFTANRQYKRAPRLLVSAEGMYYTAADGR
                     RVLDGTSGLWCVNAGHCRPKIVEAVRKQIGELDFAGSFQMGHPRAFECAAKLVNIAPP
                     GFNHVFFTNSGSESVETALKIALAYHRARGNAAKTRLIGRERGYHGVNFGGTSVGGIL
                     NNRKMFGTLLSGVDHIRHTHDLGRNAFSRGEPEYGAEFADDLERLIALHDASTIAAVI
                     VEPVACSTGVLIPAKGYLTRLAAICAKHDILLIFDEVITAFGRLGTPFGADYFGVSPD
                     IITTAKGVTNGTVPMGAVFVTGKVHDAFMNGPEEAIDFFHGYTYSGHPVACAASIATL
                     ELYKEEGLLTRAAELAPYWQEAVHSLKGLPHVIDVRNIGLIGAVEFDPVPGEPMKRAH
                     TRFVDAFERGLLVRATGDIIALSPPLIIQKKEIDQLIDIFSSVLKTAN"
     gene            7689..9185
                     /locus_tag="VFY74_04095"
     CDS             7689..9185
                     /locus_tag="VFY74_04095"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5930923.1"
                     /translation="MPTIPNAIDGRTVKSASPRVSPVYNPATGEQTAMLPLSTAEEVN
                     AAVAAAKTAASGWGATPPLKRIKFMFRFKELLDRNADEIARSISREHGKTHEDARGEL
                     ARGIDVVDFACGIPHLLKGEYSRNVGPEIDSWSDRQPLGVVAGITPFNFPAMVPMWMY
                     PVAIACGNTFVLKPSERDPSPSMLIWELFQEAGFPPGVLNVVHGDREAVDAILDHPDI
                     KAVSFVGSTPVAEHVYRRGTTSGKRVQALGGAKNHMIVMPDADMDQAVDALMGAGFGS
                     AGERCMAISVAVPVGEKTADRLVASLAPKVHALKVGPATDPESEMGPVITGEAKKRIT
                     DLIGQGVEEGAELVVDGRGLELQGYERGFFLGGSRFDRVTKDMTIYKTEIFGPVLCVV
                     RAGSYREAADLVNTHEYGNGTAIFTRDGDTARDFAASIEVGMVGVNVPIPVPVAYHSF
                     GGWKRSLFGDHSVYGPEGVRFYTRLKTVTARWPSGIRGGSEFNFPTLK"
     gene            complement(9232..9516)
                     /locus_tag="VFY74_04100"
     CDS             complement(9232..9516)
                     /locus_tag="VFY74_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="high potential iron sulfur protein"
                     /protein_id="HEX5930924.1"
                     /translation="MSNPSSLTKTQLCRRSLLKSVPMIAGAIISTSAVAETLLAQTKL
                     THEAAKYQDTPKDGQQCSACVQFVAPASCTVVEDPIAASGWCQLFVAKPA"
     gene            complement(9660..>9914)
                     /locus_tag="VFY74_04105"
     CDS             complement(9660..>9914)
                     /locus_tag="VFY74_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930925.1"
                     /translation="SAMTGDMAGAKRGAAEVIRLDPNWSVEKYLSDSGGYPDHVAMLF
                     IDAARKAGVKACVPEAELSSTPKLIHIKTCDAERAGEVAG"
BASE COUNT         1797 a         3232 c         3011 g         1874 t
ORIGIN      
        1 gggtgcgcaa gttctccttc accggctcga ccccggtcgg caagatgcta taccgccagt
       61 gcgccgacac ggttaagaag ctcaccctcg agctcggcgg caacgcgccc ctgatcgtgt
      121 tcgacgacgc cgatctcgac ccggcggtcg ccggcgccat cgcctcgaaa tatcgcaatg
      181 ccggccagac ttgcgtctgc gcgaaccgca tcctggtgca atcgggcatc tatgaccgct
      241 tcgccaaagc cctcgttgac gcggtcaaga agctcgccgt cggatcaggc ctcgaggccg
      301 gcaccaatat cggcccgttg atcaacggcg aggcgatcgc caaggtcgag acgcttgtcg
      361 gcgacgccgt caagaaaggc gcgaaggtgc tcctgggagg caagcccgac gccgccggac
      421 cgctcttcta ccggccttcg gtcgtgggcg acgtgacccc cgagatgagc atcgttcgcg
      481 aggagatttt cggtcccgtc gcggcgctcg tgcgcttcga caccgaggcc gaggcaatcg
      541 accttgccaa tgatacgccg ttcgggctcg ccgcctatct gttcagccag aatttgagcc
      601 gtgcctggcg cgtcgccgag aagatcgatg ccggcatggt cggcgtcaac gaaggcattt
      661 tttccaacga agtggtgccc ttcggcgggg tcaaggagtc gggtcttggc cgcgaaggcg
      721 ccgccgaagg cttggatgag tacctcgaag tcaaatacat ctgcctcgga gcgatcgatg
      781 cctgatcggt ctggatggac gcgaaaacga gccggcgaat gcgccttgcg cctccggcac
      841 aaccggccat actcgccggg acagacctta tcagcgaagc aagagggcaa gctcaggaac
      901 cgaccaaccg tcatgtctat aggagagcga gtatgaccgc agtacatgca gagcgttact
      961 cttcccgccg ctcgattctg aagggtcttg gcgccgccgc catcggcatc acgctctcga
     1021 gcaacaagaa attctccgct ctcgccgccg aagagccgaa gctcaatctc tacaactggg
     1081 acacgtatat cggcgagacc acgctcgaag acttcaagga cgcaagcggc atcgacgtga
     1141 acatgagcct gtttgcgacc aatgacgagc tcttcgccaa attgcgcgcc ggcaatcccg
     1201 gttacgacgt gatcgttccg ggcagcgagt tcgtcgagcg gatgatccag gccggcatgc
     1261 tcgagcccct cgaccacgcc aagattccga acatcaagaa cgtcgcgccc gagttcgtca
     1321 aagacgcgcc gtttgatcca gggcgtaagt acagcatgcc ctatacctgg ctcgtcatcg
     1381 gcatcggcta tcgcaagtcg aaggtcaagg aagtccccaa tagttggaaa tggctgttcg
     1441 attccgacgt ctacaaaggc cgcattgccg tgatgtccga cgcgagcgag ctgttccgca
     1501 actgcttcaa gtatctcggt cattctttga acgacgcttc gcccgagttg ctgaagcagg
     1561 ccgaggcgat gctcaccaag cagaagccca atatcaaggc cttccacgag gacaatggcc
     1621 aggatcttct gctctcgggc gaggtcgacg tggtgatgga atataacggc gatattgccc
     1681 agatcatgaa ggaggatgac gacgtcgact tcgtcgtgcc gaaagagggc gccatgaagc
     1741 agcaggacac gctcgccatc ccgaaaggcg caccccatcc ggacaatgcc ctcaagttca
     1801 tcaacttcat cctctcgccc gaggccgggg ccgggatcac caagacgatc ctttatccat
     1861 ctccgaatgc cgccgccgtc gcgctaatgc cggcgtccta caaggacaat ccggtgatct
     1921 tcccgccggc ggacgtcatg gcgaaatgcg agtatccgaa ctacctcggg ccggagtttt
     1981 cgcgtctcct cgacgaagcg atgacgcgac tgcgcgcgtc gtaggcaagc ggaaacgaca
     2041 tgatctagcg gggcgagagg aagcgccggg atggccactc aaacacagga gcgccggctc
     2101 gcctttttgg caacggcact gccgcccatc gcctgggtgg tggtgttctt catcgccccg
     2161 ctggccatcg tctgggccta cagtttcggc cagaacgtca gtctgaccga ggtcgatgtc
     2221 agcggcacct tcgccaacta cgcgcgggcg ctggagccgc tctatctcgg gattttcgcc
     2281 aaatcggtct tcgtggccgg gcttaccacg ctgctctgtc tcatcatcgg cttcccggtg
     2341 gcgctctcga tcgtctttgc gagcgagcgc tggcggccgt ggatgctgct tctgatcatg
     2401 ctgccgttct ggacgaacct gctcattcgc acctacgcgc tgatgtcggt gctgcgcacc
     2461 gagggctacg ccaattttac gctcgaatgg ctttggaatc agggcagctg gctcatgacg
     2521 ctggtgggct tgcagccgct cggcgagttc acgccgctgc agctcctcta caacaacttc
     2581 gccgtggtgt tggggctggt ctacgtgcat ctgccgttca tggtgctccc gctctacgcg
     2641 gcgcttgatc ggctggacaa atccctgatc gaggcgagcc tcgacctcgg cgcagggcat
     2701 ctgcgcacga tgttctcgat cgtgatcccg ctcgccatgc ccggcatcat ttccggcatc
     2761 atcatcacgt tcgttccggc gctcggtgcc tatctcaccc ccgatctcct cggcggaccc
     2821 gacagccaga tgatcgctaa tgtcatcgag cgacagttca agaaggccaa cgattggccg
     2881 ttcggcgccg cgctctcctt cctgttgatg tacgcgactt tcgcggccat tgcgtttcga
     2941 gcctatttca gccgcggcaa gcccgcggca gagcacggga tctagccatg gccgagcgcc
     3001 gcgcgcccct cgaatacaat cgtctgtggc agatgcgcgc ctggctgttt gccgttttct
     3061 tcttcctcta cgcgccgctc gttgcgctga tcgccttcag cttcaacgac tccaaacgca
     3121 ccaccgtctg gcgcggcttc accctcagat attacgagaa gctgttcagc aacgacagcc
     3181 tgctcatcgc cttcgccaac tcgctgacga tcgcgtttgt ggctacgctg atttcgctct
     3241 tcgttggtgc gctcgccgcc gtgctcctgt ggcgctaccg cttcccgggc aggctcgccg
     3301 tggagggcgc gttcacgctt ccgctcgtgg tgccggaaat ctgcatgggc gtcgccatgc
     3361 tgctgttctt cacgcgcgtc ctaccttggc ccaatgatct tatttggccg ttcaatctcg
     3421 gctcgatcat catcgcgcat gtctcgttct gcttcccctt cgtcgcgctg gtcgtgggcg
     3481 cgaggctcgc gagcttcaac aaggagcagg aggaagcggc gcgcgacctc ggcgccaacg
     3541 actggcaggc cttccgcgat gtccttctgc ctcacatcaa accggccctg atcgccggcg
     3601 ctctgctcgc cttcacgctg tcgcttgacg atttcgtcat cacctttttc acttcgggac
     3661 cggacacggt caccttcccg gtgaaggtct attcgatgct gcgattctcg gtgacgccgg
     3721 aggtgaacgc cgcgtcgacc atgctgatcg ttctcacgat cgtgctcatg gtcatcatga
     3781 tggtgatgca gagcaaaacg cagaagaagg ccaaaggatg agtaccatgg atcgagcgtt
     3841 ggccgaaggc accaagacgg ccgtgaagcc catcatcagc tttcgcgata tttccaagcg
     3901 atggggctcg acgctcgccg tcgacagtgt cagcctcgat atccagcccg gagagttctt
     3961 cgcgttgctt gggccttccg gctgcggcaa gaccacattg ctgcgcatcc tcgccggtct
     4021 cgaagtgccg tcggaaggcc atgtcctcat cgacggccgg gacatgagcg agatcccgcc
     4081 gaacaagcgc gccgtcaaca tggtgttcca gtcctacgcc gtgtttccgc atatgtctgt
     4141 cgccgacaat gtcgcctacg ggctgaagat cgatggcgtg ccgcagccgg aacgcgataa
     4201 gcgcgtccag gaagctcttg acctcgtgca actcggtcac ctggcagagc gcatgccgga
     4261 ccagctgtct ggaggtcaac gtcagcgggt agcgctttcc cgtgcgctcg tgaaaaagcc
     4321 caaggtactt ctgctcgacg agccgctctc ggcgctggac gccaaactgc gcgaggccat
     4381 gcgcttcgag ctgtctcagt tgcagcaaaa ggttgggatc acgttcatta tggttacgca
     4441 tgatcaggac gaggcgctgg cgatcgcctc gcgcatcgcg gtcatgaaca aaggatcgat
     4501 cgcccagctc ggcacgccct ccgacctcta cgagtttccc gcatcgcggt tcgtggccga
     4561 cttcatcggc tcggtgaaca tgttcgaggg caccctgctt gacgacaagc caaccgaggc
     4621 caccgtttca tgtcccgagc tcgacccgca tcgggtacac gtgccgcatt ccgtccccgg
     4681 caaggcgaag gcgccggttt gggtggcgat ccgtcccgaa aagatgacct tgcacaaaga
     4741 tgccgccgag ccccccgcgg tgcccgaatg tccggccgcg cacaacgttg ccaaaggcga
     4801 gatcaaggag atctcctatc tcggcgacat ctcgatctac cacgtccttc tcgccaacgg
     4861 ccggctgatc cgcatctcgc gtcccaatcg ctcgcgctgg gatcaggaca actttacttg
     4921 gggcgaaaag gtctgggtga gttgggccgg gtcgagcccc gttgtcctgc aaaactgacg
     4981 gctcgacgcc gagcgcgtca gcaagaaaga acgcgaacgt ccgcatgtcg atgtctgccg
     5041 aactgcatga acggaggaaa gccgcggttg cgcgcggcgt gagccacgcg agcgcgatct
     5101 acgccaagcg cgccaagaat gccgaaatct gggacgagga cggcaaccgc tatatcgact
     5161 tcggcgccgg catcgcggtg gtcaataccg gccatcagca tccgaagatc gtcgccgcag
     5221 tcgaggagca gctgaaggcg ttcagccatg tctgcttcca ggtcacgccc tacgaaacct
     5281 atgtgcgtct cgccgaacgg ctgaacgcga ttgcgccggt cgcgggccct gccaaaacca
     5341 tgctggtttc gaccggcgcc gaggccgtcg agaacgcggt caaagtcgcg cgcgcctaca
     5401 ccggccgtcc cggcatcgtg tccttcagcg gcggcttcca tggccgcacg ctcatggcca
     5461 tggcgctcac gggcaaggtg gttccgtaca agaagggctt cggcccgttc cccgccgaaa
     5521 tctacaacgt cgcttttccc gccgtttatc atggggttag cagcgagcag agccttgccg
     5581 cgctgaacgc cttgttcaag tataccgccg acccgtcaag catcgccgcc gtcatcatcg
     5641 agccggtgca gggcgagggc ggcttctaca tggcgccgct cgatttcctg cgcgccctta
     5701 gggcgctgtg cgacgagcat ggaatcctct tgatcgccga cgaggttcag acgggcttcg
     5761 cgcggacggg aaagatgttc gcgctcgagc atgccggcat cacggctgat gtcgtggcga
     5821 tggcgaaagg actggcggga gggcttccgc tcgcggcgat aaccggacgc gccgatgtga
     5881 tggacgcgtc aaatcccggc ggcctcggcg gcacatattc cggcaacccc ttggcgtgcg
     5941 ccgccgctca cgccgtgctc gaagtgatcg aagaggagga tctttgcggc cgcgccaatg
     6001 cgatcggcaa gatcattctc gaccgctgcc gccagatgaa ggaccgctcg aacctgaact
     6061 gcatcggaga tgtgagaggg atcggcgcca tgtgcgccat cgaattggtc aaggacgttg
     6121 cgagcggcga gcctgcgccg cagctcacgc agaccttgat gaaggtggca aaccagaatg
     6181 ggcttgttct tctgtcctgc ggcacgtacg gaaatgtcag tcgcttcctt gcgccgctga
     6241 cggcgagcga tgcgatcgtg cgcgagggca tggatatctt cgaggccagc ctcaccgaag
     6301 ccgtggcccg ggccgggtga ggcgcgcggc gttggacatt aggaccgata tgaacgcaaa
     6361 gcttgccacg aacgatctcg aagccttctg gatgccgttc acggcgaacc ggcaatataa
     6421 aagggcgccg cgcctgctcg tgagcgccga aggcatgtac tacaccgccg ccgacggacg
     6481 ccgcgtgctc gacggcacct cgggactgtg gtgcgtcaat gccggacatt gccggcccaa
     6541 gatcgtcgag gcggtgcgca agcagatcgg cgaactcgac ttcgccggca gcttccagat
     6601 gggtcatcca agagcgttcg agtgcgcggc aaagctcgtc aacatcgcgc cccctggctt
     6661 caaccatgtg ttcttcacca actctgggtc cgaatcggtc gagaccgcgc tcaagatcgc
     6721 gctcgcctat catcgggcgc gaggcaacgc cgcgaaaacg cggctgatcg gccgcgagcg
     6781 cggctatcac ggcgtcaatt tcggcggcac ctcggtcggc ggcatcctca acaatcgcaa
     6841 gatgttcggc acgctgctct ccggcgtcga ccatattcgc cacacgcacg atctcggccg
     6901 caatgccttt agccggggcg agcccgagta tggcgccgag ttcgccgacg atctcgagcg
     6961 tttgatcgcg ctgcacgatg cctcgaccat cgccgccgtc atcgtcgagc ccgtggcctg
     7021 ctcgaccggc gtcctgatcc cggccaaggg ctatctcacg cgcctcgccg cgatctgcgc
     7081 caagcacgac atcctcctca tcttcgatga agtcatcacc gccttcggcc gcctcggcac
     7141 gccgttcggc gccgactatt tcggcgtctc gcccgacatc atcaccacgg cgaagggcgt
     7201 caccaacggc accgttccga tgggcgcggt gtttgtgacg ggaaaggtac atgacgcctt
     7261 catgaacggc cccgaggagg cgatcgattt cttccacggc tatacctact cgggccaccc
     7321 ggtcgcctgc gccgcctcga tcgcgacgct cgagctctat aaggaggaag ggttgctcac
     7381 ccgcgcggcg gagcttgcgc cctactggca agaggcggtc cattcgctga aaggccttcc
     7441 gcacgtcatc gacgtccgca atatcgggct gatcggagcc gtcgagttcg atcccgttcc
     7501 cggcgagccg atgaagcgcg cgcacacgcg cttcgtcgac gccttcgagc gtggcctctt
     7561 ggtgcgcgcc accggcgaca tcatcgccct ttcgccgccg ctgattatcc aaaagaagga
     7621 aatcgatcag ctgatcgata tcttctcgag cgtgctgaag acggccaact gagcaacggt
     7681 acgccgccat gccgaccatc ccgaacgcga tcgacggccg cacggtcaag tcggcatcgc
     7741 cgcgcgtctc gcccgtctac aatcccgcga ccggcgagca aacggccatg ctcccgctct
     7801 cgaccgccga ggaagtgaac gcggccgtcg ccgccgccaa gacggcggcg tccggctggg
     7861 gcgcgacccc gccgctcaag cgaatcaaat tcatgtttcg gttcaaggag ttgctggatc
     7921 ggaacgcgga tgagatcgcc cgctccatct cgcgcgagca cggcaagact cacgaagatg
     7981 cgcgcggcga gctcgcccgc ggcatcgacg ttgtcgattt cgcctgcggc atcccgcatc
     8041 tgctcaaggg cgagtacagc cgcaatgtcg gtcccgagat cgacagctgg tccgaccgcc
     8101 agccgctcgg tgtcgtcgcc ggcatcacgc cgttcaactt tcccgccatg gtgccgatgt
     8161 ggatgtatcc ggtcgcgatc gcctgcggaa acaccttcgt gctgaagccg tcggagcgcg
     8221 atccgtcccc ctcgatgctg atctgggagc tgttccagga ggcgggcttt ccgccgggcg
     8281 tgctgaacgt tgtccatggc gaccgcgagg cggtcgatgc cattctcgac catcccgata
     8341 tcaaggcggt gagctttgtc ggctcgacgc cggtcgccga gcacgtctat cggcggggca
     8401 cgacgtcggg caagcgcgtg caggcgctcg gcggcgccaa gaatcacatg atcgtcatgc
     8461 ccgacgccga catggatcag gccgtggatg ccttgatggg agcgggtttc ggctcggcgg
     8521 gcgagcgctg catggcgatc tcggtcgcgg tcccggtcgg cgagaagacg gccgaccggc
     8581 tcgtcgcctc gctcgcgccc aaggtccatg ctctgaaagt gggcccggcg acggaccccg
     8641 agtccgagat ggggcccgtg atcacgggag aggccaagaa gcgcatcacc gatctcatcg
     8701 gccagggcgt cgaggagggc gccgaactcg tcgtcgatgg gcgcgggcta gagctgcaag
     8761 gctacgagcg cggattcttt ctcggtggtt cgcggtttga ccgcgtcacc aaggacatga
     8821 cgatctacaa gaccgagatc ttcgggccgg tcctctgcgt cgtgcgcgcg ggcagctatc
     8881 gggaggccgc cgatctcgtc aacacgcacg aatacggcaa cggcaccgcc atcttcaccc
     8941 gtgacggcga cacggcccgc gacttcgccg cttccatcga ggtcggcatg gtcggtgtca
     9001 atgtgccgat ccccgttccc gtcgcctatc actctttcgg cggctggaag cggtcgctct
     9061 tcggcgatca ttccgtctac ggccccgagg gcgtgcgctt ctatacgcgg ctcaagaccg
     9121 tgacggcgcg ctggccctca ggcatccgcg gcggctcgga gttcaatttc ccgaccctga
     9181 aataggtgcg tcagaaccgg ccgcgaacgt cgctcccgct tagagggatg ctcaggcagg
     9241 cttggcgacg aacagctggc accacccgct cgcggcgatc ggatcttcca ccaccgtgca
     9301 tgaagcgggc gccacgaact ggacgcaggc cgagcattgc tgcccgtcct ttggcgtgtc
     9361 ctgatacttg gcggcctcat gcgtcagctt ggtttgcgcc aacagggtct ctgcgacggc
     9421 gctcgtggag atgatggccc cggcaatcat cgggacgctc ttcaacagcg aacgccggca
     9481 aagctgtgtc ttggttaagc ttgatggatt cgacatgcga ggactccttg cccagaaagt
     9541 gagtctagcg cgggagaact tactctcctt gggaagtccc ggataacgcg tctccgccct
     9601 cggcgccgcg gcatgcggtc gcatgatccc attggttccg gtaggccacg ttaagggcac
     9661 tagccggcaa cctcgccggc cctctccgcg tcgcacgtct tgatatgaat gagcttgggc
     9721 gttgacgaaa gctcagcctc cggcacgcag gccttgacgc cggcctttct ggcggcgtcg
     9781 atgaacaaca tggcgacatg gtctgggtaa ccgccgctat cgctcagata cttctcgaca
     9841 ctccaattgg gatcgagacg aatgacttcc gctgcgcctc gcttcgcccc cgccatgtcg
     9901 cccgtcatgg cgct
//
LOCUS       DASUQP010000091         4968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_448333,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000091 DASUQP010000000
VERSION     DASUQP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448333"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..295)
                     /locus_tag="VFY74_04110"
     tRNA            complement(221..295)
                     /locus_tag="VFY74_04110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Thr,seq:ggt)
     gene            810..2099
                     /gene="murA"
                     /locus_tag="VFY74_04115"
     CDS             810..2099
                     /gene="murA"
                     /locus_tag="VFY74_04115"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5930926.1"
                     /translation="MDRIRIVGGRRLIGTIPISGAKNAALPLMIASLLTRETVTLHNV
                     PRLADVTLLGRILGNHGVDVTIAGKRSNQRSSDGQTFRLTATEIVDTTAPYEMVARMR
                     ASFWVLAPLVARCGRARVSLPGGCAIGTRPVDLHLTTLQALGAEIDLDGGYVNARAPR
                     GLTGNRIELSKISVGATHNALMAAVLARGESLIENAAREPEVADLANCLVRMGARIEG
                     IGTSTLWVQGRDTLHSAEHTVLPDRIETGTYAMAAAITGGDVLLDGAKPDLLREALDT
                     LRSTGVEIEERPTGIRVARNGSGLGPVSVETQPFPGFPTDLQAQLIALMSMADGTSQV
                     RETIFENRFMHVQELARLGASIELKGDTALVTGVSGLKGAPVMATDLRASVSLIIAGL
                     AAQGETTIGRVYHLDRGFERIEEKLGQCGAQIERLSN"
     gene            2180..2674
                     /locus_tag="VFY74_04120"
     CDS             2180..2674
                     /locus_tag="VFY74_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEX5930927.1"
                     /translation="MEPLKLIALDEEDLAVLSSLLQDAVLRVADMSYLPKQKRFAAAL
                     NRFEWEGAAKDAGQNYRRRRAALRFDRVFNAKLKNVKPQAGERVLSLLAVAFTPKDAP
                     SGTLTLTFSGDASIQLEVECIEAELKDLGPEWRTRSKPAHPGEEPDAGPKSGAGSAPG
                     AGGS"
     gene            2687..3985
                     /gene="hisD"
                     /locus_tag="VFY74_04125"
     CDS             2687..3985
                     /gene="hisD"
                     /locus_tag="VFY74_04125"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5930928.1"
                     /translation="MAAWLRTADRNFEDGFKALLGQKREQAEDVNETVASIIALVRAD
                     GDRALIDLTLKFDDLDLRKTGLRVSAAEIAAARNQVKKTTRDALQLAHDRILDHHKRQ
                     LPKDDRYRDSLGVELGTRWTPVASAGLYVPGGTASYPSSVLMNALPAKVAGVPRIVIA
                     VPAPKGVLNPLVLVAAELAGVTEIYRVGGAQAIAALAYGTETIAPVAKIVGPGNAYVA
                     AAKRQVYGQVGIDIVAGPSEVVVIADRDNDPAWIASDLLAQAEHDATAQAILITDDAD
                     FGRDVASAVEAELAVLPRAETARASWRDFGAIIVVDHLDDAPTLVDRLAPEHLEIAAA
                     DADALAARITNAGAIFIGRYTPEVIGDYVAGSNHVLPTARSARFASGLSVLDFMKRSS
                     ILKLDAEHLAALGPAAMTLAEAEGLDAHRRSVAIRLNVRD"
     gene            3989..4498
                     /locus_tag="VFY74_04130"
     CDS             3989..4498
                     /locus_tag="VFY74_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEX5930929.1"
                     /translation="MSGEHENEALKAWPEEARLVEVTLDEASIGRNTREVEHEREVAI
                     FDLLEKNCFALENHDSGPYTLKLSLAENRLVFTVGDEARTPIQHVMLSLSPFRRIVKD
                     YFLICDSYYQAIKTQPATRIEAIDMGRRGLHDEGSRLLQERLKGKIAVDIATARRLFT
                     LLCALHWKG"
     gene            4637..4855
                     /gene="infA"
                     /locus_tag="VFY74_04135"
     CDS             4637..4855
                     /gene="infA"
                     /locus_tag="VFY74_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5930930.1"
                     /translation="MAKEEMLEFPGVVTELLPNATFRVKLENEHEIIAHTAGRMRKNR
                     IRVLAGDKVLVEMTPYDLTKGRITYRFK"
BASE COUNT          955 a         1607 c         1601 g          805 t
ORIGIN      
        1 cctaaaattc gtaagcgacg agggctggta atgttccgtg agtaatccgt caaatggccc
       61 cctcccgacc gagggcgcaa cgctcaacgc actaaccgag cagaccgaag agtgcggcgg
      121 cggagaaggg gtgaggctga agaagtgggg ggctgttatc ccggcgttat cccagccggt
      181 tagacatttc caatgaaaac cacgaaagac ttggaaaacc tggtgccgcc agactggatt
      241 gaacagtcga ccccgtcatt accaatgacg tgctctacca ctgagctatg gcggctttag
      301 agaccacgcc ctagggccga ggccctatgg cttcgcaagg cggaagccat tccttgaagc
      361 ctcgcgcgcg accctgccat agcgccggaa cgcatgcaag gcggcgcacg ttctgcccca
      421 ggtcccaacc gaacggtctt ggcctatcgg ccggacgaag gagttggcca agcggcgccg
      481 ccccgccctt acggtccaga tgtaagcatg gcgctaagac tgttcgagta ggataagtcg
      541 atgaccagac cgccaaagca acaccctgcc ggaaaggcaa aatcggaggc gcgcaaggcc
      601 cggcttgcgg ccgaactcag ggcaaatttg ttgaaacgca aggctaaatc caaggaaacc
      661 caaagggatc ggaatgaggg gagacagcgt taagggcaaa ggagtagatt ggagtgttgg
      721 ccgaggcccg atcggggcct tgccagcgaa gacgggggtc ttcgccaaaa tgaaaagaac
      781 caggggcaag gacgcgaggg agtaacgtca tggaccgcat ccgcatcgtg ggcggcaggc
      841 gcttgatcgg gaccataccg atcagcggcg ccaagaacgc cgcattgcct ttgatgattg
      901 caagccttct cacccgggag acggtgacct tgcacaatgt gccgcgtctc gccgatgtga
      961 ccctgctcgg ccgcattctc ggtaatcacg gtgtcgatgt gactatcgcg ggaaagaggt
     1021 cgaaccagcg ctcgagcgac ggccagacgt tcaggctgac cgcgactgag atcgtcgata
     1081 cgaccgcgcc ttacgagatg gtggcacgca tgcgggcgag cttctgggtg ctcgcgccgc
     1141 ttgtcgcgcg ttgcggcagg gcgcgcgttt cactgcccgg cggctgcgct atcggcaccc
     1201 gccccgtcga cctgcacctc accacgctgc aagcgctcgg ggcagagatc gatctcgacg
     1261 gcggctatgt caatgcgcgg gcgccgcggg gcttgaccgg caaccgcatc gaactgtcga
     1321 agatctcggt gggcgccacc cataacgcgc tgatggcggc ggtgcttgcc cgcggcgaga
     1381 gcctgatcga gaacgcggcg cgcgagccgg aggtcgccga tctcgccaat tgcctggtgc
     1441 gcatgggagc gcggatcgaa gggatcggca cctcgacgct gtgggtgcag ggacgcgaca
     1501 cgctgcacag cgccgagcat actgtgcttc ccgaccgcat cgagacaggg acctacgcca
     1561 tggccgcagc gatcaccggc ggcgacgtcc tcctggatgg cgcaaagccc gacctcctgc
     1621 gcgaggcgct cgacacgctg cgctcgacag gagtggagat cgaggagcgt ccgaccggga
     1681 tcagggtggc gcggaatggc tcgggcttgg gtccggtgtc ggtggagaca cagcccttcc
     1741 cgggcttccc aaccgacctt caggcccagc tcatcgcact gatgtcgatg gccgacggca
     1801 cctcgcaagt gcgcgagacg atcttcgaga accgcttcat gcacgtccag gagctggcgc
     1861 gtctcggcgc cagcatcgag ctcaagggcg atactgctct cgtcaccggg gtcagcgggc
     1921 tgaagggcgc gccggtcatg gccacggact tgcgcgcttc ggtgtcgctc atcatcgccg
     1981 ggctcgcagc ccaaggggaa accaccatcg gcagggttta ccacctcgac cgcggctttg
     2041 agcggatcga ggagaagctc ggccaatgcg gcgcccagat cgaacggttg tcgaactagc
     2101 gccgccgcca caacttgacg gtttcctgcc ccgcgtaaag cgtggtaggt cgagtgcgaa
     2161 cgagccctca ccgttgccca tggaaccgtt gaaactgatc gcgcttgacg aggaagactt
     2221 ggctgtcctt tcctcgctgc tccaggacgc ggtgctccgc gttgccgata tgagctatct
     2281 gccgaagcag aagcgcttcg ccgcagcgct caaccggttc gagtgggaag gcgcggcaaa
     2341 ggacgccggc cagaactacc ggcggcggcg cgccgcgctt cgcttcgacc gcgtgttcaa
     2401 cgcgaagctc aagaatgtta agcctcaggc gggggagcgg gtgttgtcgc tgctcgccgt
     2461 cgccttcacg ccgaaggacg cgccttccgg aacgctgacg ctcaccttct caggcgacgc
     2521 ctccatccag ctcgaagtcg aatgcatcga ggccgagctc aaagatctcg gtcccgaatg
     2581 gcgcacgcgc tcaaaacctg cgcatcccgg tgaggagccc gatgccggcc ccaagtcggg
     2641 cgccggcagc gcgcccggcg ccggcggctc ttgagccgct tctcccatgg ccgcgtggct
     2701 cagaaccgcc gaccgcaatt tcgaggacgg cttcaaggcg ctgctcgggc aaaagcgcga
     2761 gcaggccgag gacgtcaacg aaacggtcgc ttccatcatc gcgcttgtgc gtgccgacgg
     2821 cgaccgcgcg ctcatcgatc tcacgctcaa gtttgacgat ctcgatctac ggaaaaccgg
     2881 tctcagagtg agcgcggccg agatcgccgc ggcgcggaac caggtgaaaa agaccacgcg
     2941 cgatgcgctt caactcgccc atgaccgcat cctcgaccac cataagaggc agctgccgaa
     3001 ggacgaccgc tatcgcgatt ccctcggggt cgagcttggc acacgctgga cgccggtcgc
     3061 ctccgccggg ctctatgtcc cgggcggcac tgcgagctac ccaagctcgg tgctgatgaa
     3121 tgcgctgccg gccaaggtcg cgggcgttcc ccgcatcgtg atagcagtgc cggcgccgaa
     3181 gggcgtgctc aaccctctgg tcctggtggc ggccgagctt gcgggcgtca ccgagatcta
     3241 ccgggtcggg ggagcgcagg cgattgccgc gctcgcttac ggcacggaga ccatagctcc
     3301 ggtggcaaag atcgtcggtc ccggcaatgc ctacgtggcc gcggccaagc gccaggtcta
     3361 cggccaggtc ggcatcgaca tagtggcggg cccctctgaa gtggtggtga tcgccgatcg
     3421 cgacaacgat ccggcctgga tcgcctccga ccttctcgct caggccgagc acgacgcgac
     3481 ggcccaagcg atcctcatca ccgacgacgc ggatttcggg cgcgacgtcg cttcagcggt
     3541 ggaagccgag ctggccgttc tgccccgcgc cgagaccgcg cgcgcgagct ggcgggattt
     3601 cggcgcgatc attgtcgtgg atcatctcga cgatgcgccc acgcttgtcg accgtcttgc
     3661 gcccgagcat ctcgagatcg cggcagccga cgccgatgcg ctcgccgcgc gcatcaccaa
     3721 cgccggcgcc atcttcatcg gccgctatac gccggaggtg atcggcgact atgtcgcggg
     3781 ctcaaaccac gtgcttccga cggcgcgaag cgcgcgcttc gcctccgggc tctccgtgct
     3841 cgacttcatg aagcgaagct cgatcctgaa gctcgacgcc gaacatctcg cagcgctcgg
     3901 tcccgcagcc atgacgttgg cggaggccga aggcctcgac gctcatcgcc gttccgtcgc
     3961 catcaggctc aatgtcagag actaggccat gagcggcgag cacgagaacg aggcgctcaa
     4021 agcctggcct gaggaggcgc ggctcgtcga ggtgacgctc gatgaggcct cgatcgggcg
     4081 caacacccgc gaagtcgagc atgagcgcga ggtcgcgatc ttcgatcttc tcgaaaagaa
     4141 ctgcttcgcg ctcgagaacc acgattccgg cccctatacg ctcaaactct cgctcgccga
     4201 gaaccgcctc gtcttcaccg tgggcgacga ggcgcggacg ccgatccaac acgtgatgct
     4261 gtcgctgtcg ccgtttcgcc gcatcgtcaa ggactatttc ctcatctgcg acagctacta
     4321 tcaggcgatc aagacgcagc ccgccacgcg catcgaggcg atcgacatgg gtcgccgggg
     4381 gctgcatgac gaagggagcc ggctcctgca ggagcgcctc aagggcaaga tcgccgtcga
     4441 tatcgccacc gcgaggcgcc tcttcacgct gctctgcgcg ctgcactgga agggctgagc
     4501 ggcgcgcttg cggctcgctg gacaccacac ccgcattctc gccgatgtcc cccacgcctc
     4561 aaggcacagg aatcgtcctt gattttgccg ccacagccga ccattctccg ccgaatttcg
     4621 attggaaagg agccttatgg cgaaggaaga aatgctcgaa tttccgggag tggtcacgga
     4681 actgctcccc aacgccacat tcagggtgaa gctcgagaac gaacacgaga tcatcgccca
     4741 tacggcggga agaatgcgca agaaccggat cagggtgctt gcgggcgata aggttctggt
     4801 cgagatgacg ccctatgatc tcactaaagg tcggatcact taccggttta agtagcaagc
     4861 tctggccgtg gcgatcgcct ttcgccaagg ccgggcaggc aaaacccgcg ccgcaggcgg
     4921 cctcccggcc ggcgaaggcc gggccgcaga ttgtgtcgcg cgcggtgc
//
LOCUS       DASUQP010000092         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_721068,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000092 DASUQP010000000
VERSION     DASUQP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721068"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFY74_04140"
     CDS             <1..1176
                     /locus_tag="VFY74_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5930931.1"
                     /translation="IAGGGIGGLAAAIALGRRGISCEILERSDFAEETGAGIQLGPNA
                     TRALAALGLLDAIESRAFKPDAIVIYDGVSGRKLSSLPLAKMAVARYGAPYLTLRRAD
                     LHAGLRAALNDLGSVTLRSGFDLAAIDAQAGDDVVARGRDGSHARGACLIGADGLWSA
                     VRAIISPAAALRFVGATAWRAQLQRANLPAPFAEPVVGLWLGPRAHLVHYPVGGGADL
                     NIVAVTAGGVTASSAARQGWNEPGSKADVLASFARWTKDAKSLLDQAEAWRGWSLYRL
                     AGLKSWTAGPVTLLGDAAHPVLPYLAQGAGLAIEDAAALAACLAAEPSDPVSAFRRYE
                     RVRKPRAARVQRTARRFGLLYHLGVPLAQARNLILARRSEATALRRFDWLYRRDEPE"
     gene            complement(1304..>2292)
                     /gene="nhaA"
                     /locus_tag="VFY74_04145"
     CDS             complement(1304..>2292)
                     /gene="nhaA"
                     /locus_tag="VFY74_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5930932.1"
                     /translation="NDGLMALFFLLVGLEIKRELRAGELDTWPRRALPLAAALGGMIA
                     PALIFLAINGRTPENWRGWAIPTATDIAFALGVLSLLGSRVPHSLKVFLTSLAIIDDL
                     GAILIIAIFYASSLSVPAVGAAIVIAAVLFGLNAVGVLRLAPYLALGVMLWVAMLFSG
                     IHATLAGVVLAMAIPLGAGAEEASGSPLQRLEDGLGSWVAFLVLPVFAFANAGVSLAG
                     ASADMLLAPLSLGIVAGLFFGKQVGIMAAVYAASRTGLGRLPEGASWRAIYGVALLCG
                     IGFTMSLFIGLLAFSDPEREATIKIAVLTGSLLSALAGVAVLLVLGRPTDAH"
BASE COUNT          429 a          758 c          746 g          359 t
ORIGIN      
        1 attgccggcg gcggcatcgg cggcctggct gcggccatcg cgctcgggcg ccggggaatc
       61 agttgcgaaa tcctcgagcg ctccgatttc gccgaggaga ccggcgccgg cattcagctc
      121 ggtcccaacg ccacgcgagc ccttgctgcg ctcggcctcc tcgacgcgat cgagtctcgc
      181 gcgttcaagc ccgatgcaat cgtcatctat gacggcgtct cggggcgcaa gctcagttcg
      241 cttccgctcg ccaagatggc ggtcgcgcga tatggcgcac cttatctcac actgcgtcgc
      301 gccgatcttc atgccggtct ccgcgcggcc ctaaacgatc tcggttccgt cactctgcga
      361 tcgggtttcg atcttgccgc gatcgacgcg caagcgggcg atgacgttgt cgcgcgcggg
      421 cgcgacggca gccacgccag gggcgcatgt ctgatcggcg ccgacggact gtggtcggcg
      481 gttcgcgcca tcatcagtcc ggcggcagcg ttgcgttttg tcggagctac ggcatggcgc
      541 gcgcaactgc agcgcgccaa tcttcccgcc ccgttcgcgg agcccgtggt cgggctctgg
      601 ctcgggccgc gtgcgcatct cgttcactat ccggtcggcg gcggcgccga tctcaacatc
      661 gtcgccgtca ccgccggcgg tgtcaccgca agcagcgcgg cccggcaggg ttggaacgag
      721 ccgggcagca aagcggatgt gctcgcaagc ttcgcgcgct ggaccaagga tgcgaaatcc
      781 ctgctcgacc aggctgaagc ttggcgcggc tggtcgcttt atcgcctcgc cggccttaag
      841 agctggacgg cgggtcccgt cacgctcctt ggcgacgccg cccatccggt gttgccgtat
      901 ctcgcgcaag gcgcgggtct cgccattgag gatgccgccg cgcttgccgc ctgtctcgcg
      961 gcagagccga gcgatccggt ttcagcgttt cgccgttatg agcgggtacg caagccacgt
     1021 gccgcgcgcg ttcaacgcac cgcgcggcgc ttcggcttgc tctaccatct tggcgtcccg
     1081 ctggcacaag cgcgcaacct catccttgcg cgaagaagtg aagcgaccgc gctgcggcgg
     1141 ttcgattggc tctaccggcg cgacgagccg gaataaggga agacggcttg gtccggaatg
     1201 acgccattac cccgtcatac cggcgaaagc cggtatccag tgcccacacc ccgtgcctgc
     1261 ccacaggatc ccggctcttg ccggaatgac gaaggtgcgc agatcagtga gcgtccgtcg
     1321 gccggcccaa aactaagagc actgcaacgc cggcaagggc cgacagcaaa gagccggtga
     1381 gaacggcaat cttgatcgtg gcttcgcgtt ccggatcgga aaacgcaagc aagccgatga
     1441 aaagactcat tgtgaagcca atgccgcaga ggagtgccac gccgtagatc gcgcgccagg
     1501 atgcgccctc gggaagccgg ccgagcccgg tcctgctcgc ggcatacacc gcggccatga
     1561 tgccgacctg cttgccgaaa aagagaccgg cgacgatacc gagcgacagc ggcgcgagca
     1621 gcatgtcagc gctcgctccc gcaagcgata cgccggcgtt ggcaaaggcg aagacgggca
     1681 gcacgagaaa ggcaacccaa gatccaagcc cgtcctcgag gcgctggagc ggagatcctg
     1741 acgcttcctc cgcgcccgcc ccgagcggta tagccatggc cagaacgaca cccgcgagcg
     1801 tggcgtggat gcccgagaac agcatcgcta cccagagcat gacgccaagc gcgagataag
     1861 gcgcgaggcg aaggacgcca acggcgttga gaccgaacag aaccgccgcg atgacgatcg
     1921 cagcgccaac ggctggtacc gagaggctcg aggcgtagaa gatcgcaatg ataagaatcg
     1981 cgccgagatc atcgataatc gcaagtgagg tcaggaagac tttgagcgag tgcggaaccc
     2041 tcgaccccaa cagcgacaag actccgagcg caaatgcgat gtcggtcgcg gtcggaattg
     2101 cccagcctcg ccaattctcc ggcgttctgc cgttgattgc taagaagatt agagccggag
     2161 cgatcatgcc cccgagcgcg gctgcgagtg ggagcgcgcg gcgcggccaa gtgtcgagct
     2221 cgccggcgcg aagctcgcgc ttgatttcca gtccgaccag caaaaagaac agcgccatca
     2281 gcccgtcgtt ga
//
LOCUS       DASUQP010000093         4476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_993704,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000093 DASUQP010000000
VERSION     DASUQP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993704"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFY74_04150"
     CDS             <1..604
                     /locus_tag="VFY74_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5930933.1"
                     /translation="DTFGAILAGAPGLSHTVLVQRLKQLERLGIVESAPKTDGRGRHY
                     ELTGAGHDLFAVCQSLGEWGARWLEIAPENLDPFVALWSMCNALRRDRLPNRRVVIRF
                     DFTGRPRRERYWLIIELGDTEICKTYPGLDEDLYITAEAEAFVKWHAGQLSWGQATRA
                     DRIRLDGSPSLVRAFPTWNARSMFAHIRPVSLTPGTSPAA"
     gene            757..906
                     /locus_tag="VFY74_04155"
     CDS             757..906
                     /locus_tag="VFY74_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930934.1"
                     /translation="MRKTEHLLFLVLMIPSILILVAAAVSMADHEIPVEPTYTIATAY
                     MAVEE"
     gene            complement(2225..2632)
                     /locus_tag="VFY74_04160"
     CDS             complement(2225..2632)
                     /locus_tag="VFY74_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5930935.1"
                     /translation="MEGPMLRGSCLCGSVRYEIRGPVGRTSHCHCAMCRKAHGAAFAT
                     YGRVERSDFAWVAGAADVASYRSSPGVTRTFCRQCGSNLQFISDKRPTTLALALGTLD
                     DDPGVQASLHIFVASKAPWFQITDELPQHAERS"
     gene            complement(2871..3824)
                     /locus_tag="VFY74_04165"
     CDS             complement(2871..3824)
                     /locus_tag="VFY74_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5930936.1"
                     /translation="MRALLATLSVAFALHAYGQDAWPTRPVRMILPFPPGGGTDILGR
                     LIAERLSAGIGQPVVTENRGGAGGNVGAEAAARSAPDGYTIVLVAPSLAISPTLYSKI
                     NYDPVKDFAPISLVATVPNVMVTQPSLPGQLQEFIAFVKAKPGALNFGSGGAGTSNHL
                     AGELFNIVIGARLVHVPYKGVNLAMQDVLAGNVHLVFIGIPAAAPHIKAGKLRALAIV
                     APQRSTALPDVPTVAEAGLKDFEVTTWYGILAPVGTPQSVVKRLNAELVKVMHAPEMK
                     EKLTATGTEPLTSTPEEFAAYIKREIVKWRDVIRRAGVKAD"
     gene            3874..>4476
                     /locus_tag="VFY74_04170"
     CDS             3874..>4476
                     /locus_tag="VFY74_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930937.1"
                     /translation="MKLTIATLSLLVSAAVQAQQKDMSFFITSVGLGKGADLGGLKGA
                     DAHCQALAKAAGAGNRQWRAYLSTDKENARDRIGKGPWRNAKGQEIAKDLNDLHKNPR
                     IDKQTGLDEKGQMVNGGGDSPNRHDILTGSTPDGRAIADKDMTCGNWTQSGEGAAMVG
                     HHDRVGLSDTEAARSWNSSHPSRGCSQDALRGSGGDGLFYC"
BASE COUNT          887 a         1390 c         1466 g          733 t
ORIGIN      
        1 tgacaccttc ggcgcgatcc tcgcgggcgc gccggggctc tcgcacaccg tgctcgtcca
       61 gcggctcaag cagctcgaac ggcttggtat cgtcgagtcg gcacccaaga ccgacgggcg
      121 cgggcgccac tatgagctca ccggcgcggg ccatgacctc ttcgcggtct gccagtcgct
      181 cggcgagtgg ggtgcgcgtt ggctcgagat cgcccccgag aacctcgacc ccttcgtggc
      241 cctctggtcc atgtgcaacg cactgcggcg agaccggctc ccgaatcgtc gcgtcgtcat
      301 ccgcttcgac ttcaccggcc gcccgcgccg cgagcgctat tggctgatca tcgagctcgg
      361 agacaccgag atctgcaaga cgtacccggg cctcgatgaa gacctctaca tcacggcgga
      421 agccgaagca ttcgtcaaat ggcacgccgg ccagctcagt tggggccaag ccacccgcgc
      481 ggaccgcatc cgactcgacg gctccccgtc gctggtaaga gcgtttccga cgtggaatgc
      541 ccgcagcatg ttcgcgcaca ttaggccggt ctccctaacg ccggggacga gccccgcggc
      601 ctgaaatgct cgttgcgggc tacttgcggg accttcgcgg aagactaccg cgggcgtcgg
      661 acgaggtgaa tacatcacca acttggcgcg ccgcacttcg ctgaccgacc cggcatcgag
      721 gcctacattg ggtccatctg atgcacggag gttgtcatga gaaagaccga gcacctgctg
      781 ttcctggtcc tgatgatccc gtcgatcctt atcctggtcg cggccgcggt atccatggcg
      841 gatcacgaaa tcccggtcga gccaacctac acgatagcga ccgcctacat ggcggtggag
      901 gagtaacgga ccggccgttg ccgacaactt caaaccgcca cgcggccaac atcaccgcgg
      961 cctcgttatg cggcgctttt cctaagcgct acgaatcgga acaactccgc gcgggccggg
     1021 tggacgttcc cggttggact tcgcctgcga tatgagaaag ccgtcgctcg agccgacaga
     1081 cttccgtcga gtttgcctcc aatcctctcc gcgtcttgta atacgcgcgc cgcgagagtc
     1141 tgagcggctc gctcattttc cgccgtatgt tttgcagccg gcttagataa agcattggtc
     1201 cgaccgtccg cactggtacc atcagtcgag gagagcaaca gatggcgaag gcgaaaaaga
     1261 aggttcggcg caagatttct cgcttaggac gtttggccac acgactggcg aagattgatg
     1321 cgaagctgcg cgccggcact ccgctaacta aaacggatgt cgcgctcttg cgattgaggc
     1381 tcgtttaagg agcggacgcc aacctgcagc gctggacagc accggccgtg acccggcgga
     1441 agccgtcggt caccaacgcg ctgttaggct gcatgcgacg agtcgagcag acgcggcgcg
     1501 acgacgtaga tcaccaacaa gccgcctgcg accagcagca tcaagtctgg cgttacacca
     1561 aggacgagag ccgccgagcc cgcaatgagc gaccacagga tggggataac cgcaagggct
     1621 cgcaattgac cgggaggagc catgagcaag cagcccgcgg ttagcaaagt agtcgggcac
     1681 ggaacggcga agagcggcag acgaggccat cgaaggccgg ttgcgacgac caagacgggg
     1741 taggccaaag cacaggtgat gagaccaatc ccaagaatct gacgcggcct cgcaccccac
     1801 tcgaatcgga gagctgatcg aactactccg aaccaaagga agagcacggc ctgtagtaag
     1861 aaggcaatcc cgaagacaac agcggccggg ttgatggggc ggaagtgata gagatgatag
     1921 gccacgccgg atgccagcca gtggatggcg agcagacccg cgactaaggt tgagatacgg
     1981 cgacgagatg ccagcagcca gtaccgtggc cgtgagcagc caaaggatga cttcaagagg
     2041 ccaaagaaga gtgttgtacg ccccgaaaac gtcgagaaac tcatcgtgag taaacggtag
     2101 tcgcatgagg ccctgggatg ccgcctaacg tccgcgtcgt ggcgcgctga cgaaggcgcg
     2161 aagcgccgct gcgcacgcgg cgctcaccaa caagcgctgt tagccgtcat ctgagctgtc
     2221 ttgattaact acgttcggca tgctgaggta gctcgtccgt aatttggaac caaggcgctt
     2281 tcgaagcaac gaagatatgc aacgatgcct gaacgcccgg atcgtcgtcg agggttccga
     2341 gcgcgagtgc aagtgtggtt ggccgtttgt cgctgatgaa ctggaggttc gagccgcact
     2401 gcctgcagaa tgtgcgagta acgccgggcg aagaccgata cgatgcaacg tcagctgcgc
     2461 cagctaccca agcgaaatcg ctgcgttcaa ctcggccata ggttgcgaag gcggcgccat
     2521 gcgcctttcg gcacatggcg cagtggcagt gactcgtgcg acccacggga ccacgaatct
     2581 cgtaacgaac gctgccgcaa aggcaactac ctcggagcat cggtccctcc atggatgacg
     2641 gctaacgtga aatcatccga acatttcggc ctatcttagc ggcggtctct tcgcagggcg
     2701 ccgtcaacga ttcctcggcc cacggcttaa gagcagtatg cagctcggtg ggattggagt
     2761 atccggatgc attcggggca aggtgcggag ccgtcgcatg aatcggcgag tggctcggac
     2821 aagaagaagc ggcaggtggt gccgccgacg cttgtgacag gagtcaatac ctaatccgcc
     2881 ttcactcctg ccctgcgaat gacatccctc cacttcacga tctcgcgctt gatgtaagcg
     2941 gcgaattctt caggcgtgct ggtgagcggc tcggtgccgg ttgcggtgag cttttccttc
     3001 atctccggcg cgtgcatgac cttcacgagc tcggcgttca gccgcttcac cacggactgc
     3061 ggcgtcccga cgggcgcgag gatgccgtac caggtggtta cttcgaagtc cttgagtccc
     3121 gcctcggcga ccgtcggaac atcaggaagc gcagtcgagc gctgcggcgc gacgatggcg
     3181 agggcgcgga gcttgccggc cttgatgtgc ggcgccgcgg cggggatgcc gatgaagacg
     3241 agatggacgt tgccggcgag cacgtcctgc atggcgaggt tcacgccctt gtaaggcaca
     3301 tgcaccagtc ttgcgccgat gacgatgttg aagagctcgc cggcgaggtg gttggaggtc
     3361 ccggcgccgc ccgagccgaa gttgagcgcg cccggtttgg ccttcacgaa cgcgatgaat
     3421 tcctgcagtt ggccggggag cgagggctgc gtgaccatca cgttcggcac ggtcgcgacc
     3481 aggctaatgg gggcgaagtc cttgaccggg tcgtagttga tcttcgagta gagcgtcggg
     3541 ctgatggcga gcgaaggcgc gacgaggacg atcgtgtagc cgtcgggcgc cgagcgcgcc
     3601 gccgcctccg cgccgacgtt gccgccggcg ccgccacggt tctcggtcac cacgggctgg
     3661 ccgatgcccg ccgagagccg ctcggcgatg agccggccga ggatgtccgt gccgccgccg
     3721 ggcgggaagg gcaggatcat gcgcaccggg cgcgttggcc acgcgtcctg gccgtacgca
     3781 tgcaaggcga aggcgacgct cagggtggct agcaatgcgc gcatggcatc ctcccttggg
     3841 aaatatctta gccgggccgg tgttcgccct gtcatgaaac tcaccatcgc caccctctcg
     3901 cttctcgtct ctgccgccgt gcaggcgcag caaaaggaca tgagcttctt catcaccagc
     3961 gtcggcctgg gcaagggcgc ggacctcggc ggcctgaagg gcgccgacgc gcattgccag
     4021 gcgctcgcta aggcggcggg cgcgggcaac cgccagtggc gcgcctacct cagcacggac
     4081 aaggagaacg cgcgcgatcg catcggcaag ggcccgtggc gcaacgccaa ggggcaggag
     4141 atcgcgaaag atctgaatga cctgcacaag aatccgcgca tcgacaagca gaccggcctg
     4201 gacgagaaag gccagatggt gaacggcggc ggcgacagtc cgaaccgcca cgacatcctc
     4261 accggctcga cgccggacgg gcgcgcgatc gccgataagg acatgacgtg cggcaattgg
     4321 acccagagcg gcgagggcgc ggcgatggtc ggccatcacg accgcgtggg cttgagcgac
     4381 acggaagcgg cgcgctcctg gaattcttcg catccttcgc gcggctgcag ccaggacgcg
     4441 ctgcgcggct cgggcggcga cggcctcttc tactgc
//
LOCUS       DASUQP010000094        10275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1130230, whole genome shotgun sequence.
ACCESSION   DASUQP010000094 DASUQP010000000
VERSION     DASUQP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130230"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFY74_04175"
     CDS             complement(<1..262)
                     /locus_tag="VFY74_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930938.1"
                     /translation="MRFVGYRLSARTISLALVAAFIALEAFLVLRTGAVGLPGLFLIR
                     VADERGTEAPRHAATPDGGESVIAKRHEPSIPSKPAGAEATGA"
     gene            982..1437
                     /locus_tag="VFY74_04180"
     CDS             982..1437
                     /locus_tag="VFY74_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930939.1"
                     /translation="MTGNGSKLPGTSARILAATLLVAASAACGTVDRVRGKDGAATEI
                     EARSVAAQDPLARPIQVGWTSARATHCGFVFNPDQLRSNFMAAEVRAGATPEQMQKIE
                     RAYDYTRESVLATIRDDLNYCNKDRTGAIRRDLNRYLAGDYAPSARMAR"
     gene            complement(1648..2457)
                     /locus_tag="VFY74_04185"
     CDS             complement(1648..2457)
                     /locus_tag="VFY74_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930940.1"
                     /translation="MTELSDELLVAYVDGQLGGKQSSAVEKVLAHDDVIARRVKALKK
                     AHRRLEAAFEAILAGEEADAASHPIPPSPGLLIPWGTVIKGAFAAAGIVVAAVLFIAG
                     LGLPLATPERNASGAADFDHVGSLARDWHEEVARAQALLGRASLEIGLDSQRNRDFIA
                     FQLGRAIGAALAPPDLGAKGYRFTRAQILRFGDEPLAQLLYLGATGAPLALYAKKGGA
                     DAAPAAKRYGALAGVAWSQAGVAYFLAGEGDEASLLELAEAIRKVGPQAER"
     gene            complement(2454..2990)
                     /locus_tag="VFY74_04190"
     CDS             complement(2454..2990)
                     /locus_tag="VFY74_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930941.1"
                     /translation="MQAEQLYDSIRTGLIAMLPRLMRFADILVGERRAGASLLGRALN
                     RMLAEEHRYQRGTALDLWAFAEIYRLWLEERHDHSGTSSRPEAGGARFAILFRNAKGH
                     PFDPVTTGFLRSLPPAQRLTLLLVYGERFDPIDAGRVLDVSGETISARLVRITGTFAD
                     RLSAGEERDAGAPREGMQ"
     gene            3164..4111
                     /locus_tag="VFY74_04195"
     CDS             3164..4111
                     /locus_tag="VFY74_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930942.1"
                     /translation="MSVAPQRSDKRKQQQAAAPAQQRAKIIASTAQIAERIAKLADET
                     AHQTKNITANAVAASKVGLARAVEIGRENVLPEIERASAKIKEHTRRDRLERDYRNYL
                     HKLHERVLDPAIESLFFKPTKDPVPLSALTIRGNNREHGHDYRPSPCRLVEWTIAGLD
                     YDLSRLTFVDHGAGKGRVLLLASQYPFNAIGGVEFAEELHDDATMNIAQFPRSRMKCR
                     NVECVLDDASALGPPEGEAVHYFFNPFSREVFAEVLDNIVVSYRSRPRRLYLILVDPV
                     AADLIDQSGVFQRYAMPQLERLKVKALSPYEVAFYRSLA"
     gene            complement(4126..5154)
                     /locus_tag="VFY74_04200"
     CDS             complement(4126..5154)
                     /locus_tag="VFY74_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5930943.1"
                     /translation="MSDNVITTDVVIVGAGPVGLFAVFELGLVDVKCHLVDILDRPGG
                     QCTELYPEKPIYDIPGLTVVTGQELTDRLMEQIKPFGAEFHFNQRVDSLERRNGSFRL
                     ATDAGTVLESKVVVIAAGGGSFTPKRPPLPGIEQYEGTSVFYSVRKMENFKGRDVLVV
                     GGGDSALDWTLNLQPVAKSLTLLHRRAEFRAAPASVQKMFQLVEQGDVSFKLGQVTAL
                     HGEGGQLRAVTVKGSDGATFELPCEVLLPFFGLTMKLGPVADWGLNLDENLIPVDTAK
                     FETSEKGIFAVGDINTYPGKLKLILSGFHEAALMAQQAHHYVYPDKKLLFQYTTSSTS
                     LQKKLGVK"
     gene            complement(5162..5482)
                     /locus_tag="VFY74_04205"
     CDS             complement(5162..5482)
                     /locus_tag="VFY74_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5930944.1"
                     /translation="MARITFIQNDGTERVVEADPGMTVMETAVKNGIAGIAAECGGAC
                     ACATCHVYVDEAWRETTGAPEQMEEDMLDFAFDVRPSSRLSCQIKVTEALDGLVVRIP
                     DKQF"
     gene            6155..9739
                     /locus_tag="VFY74_04210"
     CDS             6155..9739
                     /locus_tag="VFY74_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930945.1"
                     /translation="MAQEQAKSPPEAAAAGPKDKSQNPLEAMMTRSREEKPGDNPPAE
                     RGFDLPKRTSLAERLKAEQRKRDGGEPKREESAKAPEAAPKPPLEFSEPQATSASVPP
                     KQFVRTRRPAGPPPQRRLSAPANDDFPSIGGLIFALQQRPSRTPFLVALGASVVWFLI
                     GGFFAFGLISNQVSSAGVSGLVGSSSALTAALAIIVPIVIFWFLALLVWRAQELRLMA
                     SAMTEVAVRLAEPDRLAEQSVASVGQTIRRQVAAMNDAISRAIGRASELEAMVHSEVA
                     ALERSYGENEHRVRNLISELVTEREALANNSQRVSDALKGVGAQIARDITAASASIDK
                     KLAERGVQLTELLVSRSSEAAEQVHKAQARVADAVPALVERLSKEQVRLTQVIEGATQ
                     NLASLEGSIVQRTSALDKTMKERTETLQASLASRIEGLENSVAQGALLLDKTMKDRTD
                     AFTGLLSQGAVLFDNTLKMRTDDLTVAIAEGAEVLDKTMKDRTDGFTNLVSQGAVLFD
                     NTLKQRTDELTVAIGEGAGALDKTMKDRTDAFTNLVSQGAVLFDNTLKQRTDELTVAI
                     GEGAGALDRTLRDRTDSFMGLVSQGAVVLDKTLTDRTGFFNAMIEQKAQLMDKQLHDR
                     TTQFLTAINQGAIALDQTLADRAETFTTSLFQRVKALETAIGQQTATLDRTMTERAQA
                     VIVALAERLQAIDTTFSHRTNEADRMLGEHARATAETFGKQTAQLGQVLANNSAMIQQ
                     TANQVGAQSREAVSVLTSQTQTLREVSRGLLEQIHGLTQRFENQGQAILTAAKALDSS
                     NTKIDSILEGRHQSIIALLHTVNTKAQDLDGMMRSYAGMVENAMTQAEARAKQVGTAL
                     TRDTSGQAQAALAQIERLREEAQAHTARAVNDLKGSFETVITQIGRQLEQMRGQFDNT
                     SRGMREAAQQTASDLDSLRQEMQRRMEALPEHTAQATAAIRKALSEQLREIEAITPVL
                     TRPIPPQPAAPETFRQAQAPRPPQAQFDAGTFPHFDARGRQVAPPEAGEMGQVAGGLA
                     QQLAGASYTPRPQAQQGAPWPPADLAARVAQSADPAYAGGAQAPGARPAAPAQTGGQL
                     RLDEISRAIDLRTATEFWQRFRAGDRAALGRHIYNPDGQATFDEISRRYGRENEFRVT
                     VDRYIADFERLLGEAEASDPEGRMLQNYLTSESGRVYLLLSHASGRLR"
     gene            complement(9903..>10275)
                     /locus_tag="VFY74_04215"
     CDS             complement(9903..>10275)
                     /locus_tag="VFY74_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5930946.1"
                     /translation="DQVSYYKDVAWSDRLPRLVQARIIETFQNSGAVKAVSGSTGQFA
                     LVTDLRAFQIDVTSGKAYAEIEIFAKLVNTASGKVISTKGFSARVPATTDSPGDAIAA
                     LNQAFTEVLQDTTTWVATRKA"
BASE COUNT         1928 a         3402 c         3275 g         1670 t
ORIGIN      
        1 cggcccccgt tgcctccgcc ccagcgggct tcgacgggat ggacggctcg tgacgtttgg
       61 cgatcacgct ctcgccaccg tcaggcgttg ccgcgtgtcg cggcgcttcg gtaccgcgct
      121 catcggcaac gcggatgaga aagagtccgg gaagtccgac cgcgccggtg cgaagcacga
      181 gaaacgcttc caacgcaatg aacgccgcaa ccaacgcgag actgatcgtg cgggcactca
      241 ggcggtagcc cacgaaacgc atgatagatc caaaaaaaag aggccccaag agacgcgcgg
      301 aaacgccgcg catcctgagg ctattctagt gaaccgagaa ggcaaagaaa gggcttcgac
      361 tgcgagcgtc gttcttcgac ctgcccgtcc tgcaggcgcc cgagagcgat ccagtggata
      421 gcggggaaaa gggcgtgttt cggaatgcgc acgcttctgc ctcatgtatg gtttgtttgt
      481 gagcggactg gttatggaag gcgccggcgg agaaattcgg cggctccggg aagtagccgg
      541 aaagctcact gggcaggcaa gcccggggtt gcccattgcc atgggcagcc caaggcggag
      601 ggggcgtaag cgcaaagtgc cacggtctgg cggcctcgcc gcaataccgt gaggcggagt
      661 cgccggggta ttttgcgcca caatccttcc gctgattggc tttggccggg ccaaggcttg
      721 agccacgtcg tagcaacgcg acgctaaaca aggaccgtgg tcgggtcacg ccgccgttcc
      781 atgggagacc atggggccga gcgcgtgatc cagagaccga aaggtctcgc ggcgccgacc
      841 gatcgtcccg aacgggcgtg aggctcgaca tcgcacaggg aggcggggct ccaaggctcc
      901 gcctttcctg ttctttgcgc ctgctccacg cgtctcacgc ccctttagcg cttcacccct
      961 ccccgctaag atcgccccgc catgacgggg aatggttcga agctgccggg aacgagcgcg
     1021 cgcattcttg ccgcgacgct cctcgttgcc gcctctgcgg cctgcggcac ggtggatcgc
     1081 gtcaggggca aggacggcgc cgcgaccgaa atcgaagccc gtagcgtggc ggcgcaggat
     1141 ccgctggcgc ggcccattca ggtcggatgg acttcggccc gcgcgaccca ttgcggcttc
     1201 gtgttcaatc cggatcagct caggtcgaac ttcatggcgg cggaagtcag ggccggcgcg
     1261 acgcccgagc agatgcagaa gatcgagcgc gcctacgact acacgcgcga atccgtcctc
     1321 gccacgatca gggacgatct caactattgc aacaaggacc gcaccggcgc gatcaggcgc
     1381 gatctcaatc gttaccttgc cggcgactac gcgccttccg cgcgcatggc gcggtagaga
     1441 tctcctctcc cttgagggat agggcttccc gcccggcgcg cgaagcgcgg cgaatggcgg
     1501 gaagggtgag ggtccccctc accgctcgct gcgctcgcgt gctctcccta agggaagagc
     1561 gggagtttac cgcgccgtca agccgtcatc cttcgagggc cgcttcgcgg gccacctcag
     1621 gatgacgagc cccggtatcc gctggcgcta gcgctcagcc tgcggcccaa ctttccggat
     1681 cgcttcggcg agctccagga gcgaggcctc atcgccctcg ccggcgagaa aataggcaac
     1741 gcctgcttgc gaccacgcca ctccggccaa cgcgccgtag cgcttcgcgg ccggtgcggc
     1801 atcggctccg cctttcttgg cgtaaagcgc aagcggcgct ccggtcgcgc caaggtaaag
     1861 gagctgcgcc agcggttcgt cgccgaaacg caagatttgc gcgcgggtga agcgatagcc
     1921 tttcgcgccg aggtcgggcg gcgcgagcgc ggcgccgatg gcgcgcccga gctggaaggc
     1981 gatgaaatcc cgattcctct ggctatcgag cccaatctcg aggctggcgc ggccgagaag
     2041 cgcctgcgcg cgcgcaactt cctcatgcca atctcgcgcg aggcttccga catggtcgaa
     2101 gtcggcggcg cctgacgcat tgcgttccgg ggtcgcgagc ggcaaaccaa gtccggcaat
     2161 gaagaggact gcggccacga cgattcctgc ggcggcgaag gcgcctttga tgaccgtgcc
     2221 ccaggggatc aacagacccg gcgaaggcgg gatcggatgt gaagcggcgt cggcctcttc
     2281 tccggcgaga atggcctcga acgccgcttc gagcctgcga tgcgccttct tcagggcctt
     2341 gacgcggcgg gcgatgacgt cgtcgtgcgc cagcaccttc tccaccgcac tcgactgttt
     2401 gcccccaagt tgcccatcga cataggcgac aagaagttcg tcgctgagct cggtcattgc
     2461 attccctcgc ggggcgcgcc agcgtcgcgc tcctcgcccg cactgagccg gtcggcgaaa
     2521 gttccggtga tgcgcacaag acgcgcgctg atggtttcgc ccgacacatc gaggacgcgg
     2581 cccgcatcga tgggatcgaa gcgctcaccg tagacgagga gcagggtcaa gcgctgcgcc
     2641 ggcggaaggc tgcggagaaa tccggtggtg acgggatcga acggatgtcc cttcgcgttg
     2701 cggaacagaa ttgcaaagcg cgcaccgccg gcctctggcc ggctcgaggt accgctgtga
     2761 tcgtggcgct cctcgagcca gagccggtag atctcggcga aggcccagag atcgagcgcg
     2821 gtaccgcgct ggtaacggtg ctcttcggcg agcatgcgat tgagcgcgcg gcctagaagg
     2881 cttgcgcccg cgcgccgttc gccgacaaga atgtcggcaa aacgcatcag ccgcggcagc
     2941 atggcaatga gcccggttct aatcgaatcg tagagttgtt ccgcctgcac ctggccgttc
     3001 cccgtagctt gggtgcgtgc tggccccccg ggaatacccg gcgaatcaag aggatagcat
     3061 atttcagccg gcggcaggac ggccgcggaa aagcttcaga aaaagcactt gcgtcctgcc
     3121 cttaagctgc ccaatgcttg tgcaaaacgc ggggttcatg cgcatgtccg tcgcccccca
     3181 acgctccgat aagaggaagc agcagcaggc tgctgcgccc gcgcagcagc gcgccaagat
     3241 tattgcctcg accgcgcaga tcgccgagcg catcgccaag ctcgccgacg aaaccgccca
     3301 ccagaccaag aacatcacgg cgaatgccgt ggccgcgagc aaagtgggac ttgcccgcgc
     3361 ggtggagatc gggcgcgaga acgtgttgcc cgagatcgag cgcgccagcg ccaagatcaa
     3421 ggagcacaca cgccgcgacc ggctggagcg agactaccgc aactatctgc acaagctgca
     3481 tgagcgcgtg ctcgatcctg ccatcgagtc gctattcttc aagccgacca aggatcccgt
     3541 gccgctctcc gccctcacca tacgcggcaa caatcgcgag cacggccacg actaccggcc
     3601 gtcgccgtgc cgcctcgtcg aatggacgat cgccgggctc gactacgatc tgtcgcggct
     3661 gaccttcgtc gatcacggag cgggaaaggg acgcgtgctg ctgcttgcct cgcaataccc
     3721 cttcaatgcc atcggcggcg tcgagttcgc cgaggagctg catgacgacg cgaccatgaa
     3781 catcgcccag ttcccgcgca gccgcatgaa gtgccgcaac gtcgaatgcg tgcttgacga
     3841 tgcaagcgcg ctcgggccgc cggaaggcga ggccgtgcac tacttcttca acccgttctc
     3901 gcgcgaagtc ttcgccgagg tcctcgacaa tatcgtggtg tcgtaccgga gccggccgcg
     3961 ccggctctat ctcatcctcg tcgatccggt ggcggccgac ctcatcgatc agagcggcgt
     4021 gttccaacgc tacgccatgc cgcagctgga gcggctcaag gtgaaggcgc tcagcccgta
     4081 cgaggtcgca ttttaccgct cgctggccta gcgctcaggg cgttcctact tgacgccgag
     4141 cttcttctgc aggctcgtcg acgaggtggt gtactggaag agcagcttct tgtcgggata
     4201 gacgtagtgg tgcgcttgct gcgccatcag cgccgcctcg tgaaatcctg acaggatcaa
     4261 tttcagcttg cccggatagg tgttgatgtc gccgacggcg aagatgcctt tttcgctcgt
     4321 ctcgaatttc gccgtgtcca ccgggatcag attctcgtcg agattgaggc cccagtcggc
     4381 gacgggcccg agcttcatgg tgaggccgaa gaagggaagc aggacctcgc acggcagctc
     4441 gaaggtggcc ccgtccgagc ccttcaccgt aaccgcgcgg agctgcccgc cctcgccgtg
     4501 cagcgcggtc acctggccga gcttgaagct cacgtcgccc tgctccacga gttgaaacat
     4561 cttctgcacc gatgccggcg cggcgcggaa ctcggcgcgg cggtgaagca gcgtcagaga
     4621 cttggcgacg ggctgaaggt tcaaggtcca atcgagcgcg gaatcgccgc cgccgacgac
     4681 gagcacgtcg cggcccttga agttctccat cttgcgcacc gaatagaaga cggaggttcc
     4741 ttcgtattgc tcgatgcccg gaagcggcgg acgcttgggc gtgaacgatc cgccgcccgc
     4801 cgcgatcacc accaccttcg attcgagaac ggtgccggcg tcggttgcga gcctgaagct
     4861 gccgttccgc cgttccaggc tgtccaccct ttgattgaaa tgaaactcgg cgccgaaggg
     4921 cttgatctgc tccatgagcc gatcggtcag ctcctggccc gtgacgacgg tcaaacccgg
     4981 aatgtcgtag atcggcttct cgggatagag ctcggtgcat tgcccgccgg ggcggtcgag
     5041 gatgtcgacc aaatggcatt tgacgtcgac gagaccaagc tcgaagacgg cgaacagccc
     5101 gacgggcccc gcgcctacga tcacgacgtc tgttgtgatg acgttgtcgg acatttgcgg
     5161 ctcagaattg tttgtcggga atgcgcacga cgaggccgtc gagcgcctcg gtgaccttga
     5221 tctggcagga gagccggctc gacggcctca catcgaaggc gaagtcgagc atatcttcct
     5281 ccatctgctc aggggcgccg gtggtctcgc gccatgcctc gtccacatag acgtggcatg
     5341 tggcgcaggc gcaggctccc ccgcattcgg cggcgatgcc ggcgatccca ttcttcaccg
     5401 ctgtttccat cacggtcatg cccggatccg cttcgaccac gcgctcggtc ccgtcgttct
     5461 gaataaaggt aatcctggcc atgacattgg cgggcttcgg tgtcttccga tctcggggac
     5521 cgggcggcgg ttggctcgcg cccgaaaggt gaaggcgcga gaaggagccc ccgctcgcgg
     5581 cccgtgctct ataggacaat tgaatttgag actccagcgc cttcgcggcg tcaaatcttc
     5641 gcgctttcgt ggtgatcttg ccgcgggggc gcaccctaga tttgcgcatt gcgagcctga
     5701 aattcaatcg cgccgaaacg cccgctcgac gcagataggc tcggcgagaa cgtctctcac
     5761 acgccatcgg gcgatggcgt gtgtgcaaga ggcgcgaggg cggagagact tgagagcgca
     5821 ggatcgctgc cagaaaaacg ccgaggcaat gttaccttac tgcaaccagt ggcccctgcc
     5881 gggccggcgg tcgcccccca ggtcgtcgaa attaccaaca atctgaatgc cttatggtgc
     5941 gtcaaccatg gacaccgatt aaactttttt tagccctaaa aattcgttaa cgattacaac
     6001 acagggggag atgcgtcggt gcccttccgg ataaatcatg cgtccggaaa tatgggctgt
     6061 acgtctatga cgcttggcgc cgcagctggc gggtatcgtg tagcgaggcg caaaaattct
     6121 ctcatgagtt ttcgtcggtt gcgaggaacg cgtgatggcg caagagcaag cgaagtcgcc
     6181 gccggaagct gcggcagcgg ggcccaagga caagtcgcaa aacccacttg aagcgatgat
     6241 gacccggtca cgcgaggaaa aaccgggcga caatcccccc gccgagcggg gcttcgacct
     6301 cccaaaacgg acctctctgg cagaacggct caaggccgag cagcgcaagc gcgacggcgg
     6361 cgagccaaag cgcgaagagt cggcaaaggc gccggaagcg gctccgaagc cgcctttgga
     6421 gttctccgag ccacaagcca cgagcgcctc cgttccgccc aagcaattcg ttcgcacccg
     6481 gcgtccggcg gggccgccgc cgcaacgccg tctttccgcc cccgccaatg acgatttccc
     6541 ctcgatcggg ggcctgatct tcgcgctcca gcaacggcca tcacgcacgc cgttcctggt
     6601 ggcgctcggt gcatcggtcg tctggttcct catcggcggc ttcttcgcgt tcgggctgat
     6661 ctcgaaccaa gtgtcttcgg ccggcgtttc ggggctcgtc ggcagctcct ctgcactcac
     6721 cgcagcactt gccatcatcg tccccatcgt catcttctgg ttcctcgccc ttctcgtttg
     6781 gcgcgcgcag gaactgcgcc tcatggcttc ggccatgacc gaggtcgcgg tgcgtctcgc
     6841 cgagcccgac cggcttgcgg agcagtcggt cgcttcggtg ggacagacga tccggcggca
     6901 ggtcgccgcc atgaacgacg ccatctcccg cgccatcggc cgggcgagcg aacttgaggc
     6961 catggtgcat agcgaggtcg ccgcgctgga gcggtcctat ggcgagaacg agcaccgcgt
     7021 caggaacctg atcagcgaac ttgtcaccga gcgcgaggcg ctcgccaata acagccagcg
     7081 cgtgagcgat gcgctcaagg gcgtcggcgc ccagatcgcg cgcgacatca ccgcggcgtc
     7141 ggcctcgatc gacaagaaac tcgccgaacg cggcgttcaa ctgaccgagc ttctcgtctc
     7201 ccgttcgagc gaggccgccg agcaggtcca caaggcccag gcgcgcgtcg ccgacgccgt
     7261 gccagccctg gtcgagcgcc tgagcaagga gcaggtgcgc ctcacgcagg tgatcgaggg
     7321 cgcaacccag aatctcgcgt cgctcgaagg ctcgatcgtg cagcgcacaa gcgcgctcga
     7381 caagaccatg aaagagcgca ccgaaacgct gcaagcgtcg ctcgcgtccc gcatcgaggg
     7441 gcttgaaaac tccgtcgccc agggcgccct cctcctcgac aagacgatga aggatcggac
     7501 cgacgccttc accggtctcc tgtcgcaagg cgccgtgctg ttcgacaaca ctctgaagat
     7561 gcgcaccgac gacctgacgg tggcaatcgc cgagggcgcc gaggtcctcg acaagacgat
     7621 gaaggaccgg accgacggct tcaccaacct cgtctcccag ggcgcggtgc tgttcgacaa
     7681 caccctgaaa cagcgcacag acgagctgac cgtggcgatc ggcgaaggcg cgggcgcgct
     7741 cgacaagacg atgaaggacc ggacggacgc cttcaccaac ctcgtctccc agggcgccgt
     7801 gctgttcgac aacacgctaa agcagcgcac cgacgagttg accgtggcga tcggcgaagg
     7861 cgcgggcgcg ctcgacagga cgctgaggga tcgcaccgat tcgttcatgg gcctcgtatc
     7921 ccaaggcgcc gtcgtgctcg acaagacgct caccgaccgc accgggttct tcaacgccat
     7981 gatcgagcag aaggcgcagc tgatggacaa gcagctgcac gatcgcacga ctcagttctt
     8041 gaccgcgatc aatcagggcg ccatcgctct cgaccagacg ctggccgacc gggccgagac
     8101 tttcaccacc tcgctgttcc agcgggtgaa ggcgctcgag accgccatcg gtcagcagac
     8161 cgcaacgctc gacaggacca tgaccgagcg ggcgcaagcc gtgatcgttg cgcttgccga
     8221 gcggctccag gcgatcgaca ccactttcag ccatcgcacc aacgaggccg accggatgct
     8281 cggcgagcac gcgcgcgcaa ccgccgagac cttcggcaag cagacggcac agctcggtca
     8341 ggtgcttgcc aacaactcgg ccatgatcca gcagaccgca aatcaagtcg gcgcgcagtc
     8401 gcgcgaggcg gtctccgtcc tcaccagcca gacccagacg ctgcgcgagg tgtcgcgcgg
     8461 gctgctcgag cagattcacg gcctcaccca gcggttcgag aaccagggac aggccatcct
     8521 caccgccgcc aaggcgctcg attcctccaa cacaaagatc gactcgatcc tggaagggcg
     8581 gcaccagtcg atcatcgcac tcctgcacac ggtgaacaca aaggcgcaag acctcgacgg
     8641 catgatgcgt tcctacgcgg gcatggtcga gaacgccatg acccaggcgg aagcccgcgc
     8701 gaagcaagtc ggcaccgccc ttacccgcga tacgtcgggt caggcgcaag ccgcgctcgc
     8761 ccagatcgag cggctccgcg aggaagcgca ggctcacacc gcgcgcgcgg tcaacgacct
     8821 caagggcagc ttcgagacgg tcatcaccca gatcggccgg cagctcgaac agatgcgcgg
     8881 ccagttcgac aacacctcgc gcggcatgcg cgaagcggcg cagcagacgg cgagcgatct
     8941 cgacagtctg cgccaggaga tgcagcggcg catggaggcg ttgcccgagc atactgcgca
     9001 agcgacggcc gccattcgca aggcgctcag cgagcagctg cgcgaaatcg aggcgatcac
     9061 gccggtcctg acccgtccca tcccgccgca gcctgcggcg cccgagacct tccggcaggc
     9121 ccaggcgccc cgcccgcccc aggcccaatt cgatgcgggc accttccccc atttcgacgc
     9181 gaggggacgg caggtcgccc cgcccgaggc cggcgaaatg ggacaggtgg cgggcggtct
     9241 cgcccaacaa ctggccgggg cctcctacac gccgcgcccg caagcacaac agggcgctcc
     9301 atggccgccc gccgatctcg ccgcgcgggt cgcacagagc gcagatccgg cctacgccgg
     9361 cggagctcaa gcgcctggcg cccggcccgc ggcgcccgct caaaccggcg gtcagctccg
     9421 tctcgacgag atttcccgcg ccatcgacct ccgcacggcg acagagttct ggcagcgctt
     9481 cagggcgggc gaccgcgccg cgctcggacg ccacatctac aatcccgacg gacaggccac
     9541 cttcgatgaa atctcccgcc gctatggccg cgagaacgag ttccgcgtca ccgtcgaccg
     9601 ttacatcgcc gatttcgagc ggctgctcgg cgaagccgaa gcctcagacc ccgaggggcg
     9661 gatgttgcag aactatctga cctccgagtc gggccgcgtg tatctcttgc tttcgcatgc
     9721 gagcggacgg ctgcgttaaa cgaggcgcgc aaagctcctt taaggcgaag ttcccttagt
     9781 tttgggcgcg gatatttctc atcatgcgtt gtcatgcccg cggaagcggg catccagtaa
     9841 actaaagcct cggatgatag cgcgcgcaat gacatcaccg ggggagacag aaagagagcc
     9901 cactaggctt tgcgggtggc gacccacgtc gtggtgtctt gcagcacctc ggtgaaagcc
     9961 tggttcaagg cggcgatggc atcgcccggc gaatcggttg tggcggggac ccgcgcgctg
    10021 aatcccttgg tcgaaatcac tttgccgctg gcggtgttga ccagcttggc gaagatctcg
    10081 atctcggcat aggccttgcc gctcgtcacg tcgatctgga aggcgcggag atcggtgaca
    10141 agagcaaatt gaccggtcga gccgctgacg gccttcaccg ctccggaatt ctggaacgtc
    10201 tcgatgatgc gcgcctgcac caggcgcggc aggcgatcgc tccaggcgac gtccttgtag
    10261 taggagactt gatcg
//
LOCUS       DASUQP010000095         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_995655,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000095 DASUQP010000000
VERSION     DASUQP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995655"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..2983
                     /gene="topA"
                     /locus_tag="VFY74_04220"
     CDS             386..2983
                     /gene="topA"
                     /locus_tag="VFY74_04220"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5930947.1"
                     /translation="MNIVIVESPAKAKTVNKYLGPSYRVLASYGHVRDLPSKNGSVLP
                     DKDFEMHWDVEPKAAKRLDEIAKAVKGANKLILATDPDREGEAISWHVLQVLDRKKAL
                     AGVPVERVVFNAVTKEAVLDAMRHPRAIDRPLVNAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRLVCDRELEIEQFKSQEYWSILAELATAKSEIFQARLISADGKK
                     LEKFDIADEATALRLKAALEGGRFRVANIESKSQRRNPAAPFTTSTLQQEASRKLGFS
                     PRQTMQLAQRLYEGVDLGGEAEGLITYMRTDGVQIVHEALASIRKLVTKRYGDRYVPS
                     SPRQYESKAKNAQEAHEAIRPTDFSKDPDAVARYLEADAAKLYRLIWQRTLASQAASA
                     EIERTTADIDVAGKDGKRYGVRATGSVIRFDGFLKIYEEGIDDAMGEDGALPALAKGD
                     ALDPRKIEAKQHFTEPPPRYSEATLIKKMEELGIGRPSTYASTLNVLRDREYVRLDKK
                     RLYPEDKGRLVTAFLESFFSRYVEYDFTADLEEKLDLISAGEIEWKDVLRDFWREFIA
                     AVNDIGDLRIAEVLDALNDLLGPHIFPDQGAGADPRACPSCASGRLSLKVGKFGAFIG
                     CSNYPDCRYTRQLADTNGEMASLNEGKLLGTDPETGLGITLRTGRFGPYVQLGEHDGG
                     RGEKPKRASIPKGNDPQEVDLDRAIALLSLPRDVGLHPESGKSITAGFGRFGPYIQHD
                     GKYASLGGPEEVFEIGLNRAISLLAEKAASSRARRGANVIRDLGAHPALGGKVQVLTG
                     RYGPYVKHGKVNATLPRDREPDEVTLEEAVALIAAKSAKGASKKTTPKKPARKAGKSN
                     KEAVEETAG"
     gene            2993..>4694
                     /locus_tag="VFY74_04225"
     CDS             2993..>4694
                     /locus_tag="VFY74_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX5930948.1"
                     /translation="MAKLKGAPRKKTAGKLPSRQDILDFLATSSSAAGKREIARAFGV
                     KGGDRIALKNMLRQMADDGLIAGSRRKLMRPGVLPPVTVIEVTARDRDGEFIARPAVW
                     DDAQGPAPRILMVEGKREADPVAGIGERVLARITARSGDADYPYQARTIKRLARAARS
                     LLGIFRALPGGTGVIDPVDRKQLKEWPVPRGATGDAAHGELVRFEIARASRMGVQSAR
                     VTELLGNPEAEQMTSLIAVHAHGIPDRFPEAALAEAASAMEPDLKRREDLRHLPLVTI
                     DPSDARDHDDAVWAEADPNPANRGGWVVIVAIADVASYVRSGSALDKEARKRGNSVYF
                     PDRVVPMLPERISNDLCSLVAHEPRPCLAVRMVFDKDGTKIGHRFLRAVMRSAASLTY
                     EQAQAAIDGRLDEMTAPLLETVLRPLWGAYASLNFARTGRGPLELDLPERKILLDDAG
                     RVRGVATPPRLDAHRLIEEFMIAANVAAAETLEAKRSPLIYRVHDSPSKEKLASLGEF
                     LATIGIKLPKSGTLKPIHFNRVLTETRATPTAELDGEVILRRQAQAENAVGHFGQFGL
                     N"
BASE COUNT          864 a         1574 c         1505 g          751 t
ORIGIN      
        1 caccgccttg tttttgcgcc gattgggttc ttgcgccgat tgacgttcga ccggacccca
       61 gaagcctcct ctccggccgc ggcggtccct aaaaagcatc catttggctt ggccggggcc
      121 tcgccggcaa ctatgtcagt cacgcgttag cccactggtt caggtggcgg ggcgtttagg
      181 gtgctgtccg gccatggttc aatatggcct tgaaaagtcc cgattctggg gccttttgcg
      241 caaagccagg ccaagatagc gcaccgcctc gcccccgcca gatttgacag ccgcctcgca
      301 aaacaccatt ttccgggcca taggcggcgg ccgccggccc ctctgccctc agcccgacgc
      361 aatttgcgcg gctaggaacg tctcaatgaa cattgtcatc gtagaatcgc cagccaaggc
      421 aaagaccgtc aacaagtacc tggggcccag ctatcgcgtg ctggcctcct acggtcatgt
      481 gcgcgatctc ccctcgaaaa acggctcggt cctccccgac aaggatttcg agatgcattg
      541 ggacgtcgag cccaaggctg ccaagcggct ggatgagatc gcgaaggccg tgaagggcgc
      601 gaacaagctg atcctcgcga ccgaccccga ccgggagggc gaagccattt cctggcatgt
      661 tctgcaagtg ctcgaccgca aaaaggcctt ggccggcgtg ccggtcgagc gcgtggtctt
      721 caatgccgtg accaaggagg cggtgctcga cgcgatgcgc catccgcgcg cgatcgaccg
      781 gccgctcgtc aacgcctatc tcgcccgccg cgcgctcgac tatcttgtgg gcttcacgct
      841 gtctccggtc ttgtggcgga aattgccggg cgcgcgctcc gccggccggg tgcaatccgt
      901 ggcgcttcgg ctcgtctgcg atcgcgagct cgagatcgag cagttcaagt ctcaggaata
      961 ttggtcgatc ctggccgagc ttgccaccgc caagagcgag atcttccagg cgcggctcat
     1021 ctcggccgac ggcaagaagc tcgagaagtt cgatattgcc gacgaggcta ccgctcttcg
     1081 cctgaaggcc gcgcttgaag ggggacgctt cagggttgcc aacatcgaaa gcaagtctca
     1141 acgccgcaat cccgcagcgc cgtttaccac ctcgaccctc cagcaggagg catcgcgaaa
     1201 gctgggcttc tcgccgcgcc aaaccatgca gctcgcccag cgcctctacg agggtgtcga
     1261 tctcggcggc gaggccgagg gtctcatcac ctatatgcgg accgacggcg tgcagatcgt
     1321 gcacgaggcg ctcgcgagca ttcgcaagct ggtcaccaag cgctatggcg atcgctatgt
     1381 gccctcctct ccgcgccaat acgagagcaa ggccaagaac gcgcaagagg cgcatgaggc
     1441 catcaggccg accgacttca gcaaagatcc cgatgcggtt gcccgttatc tcgaggccga
     1501 cgccgccaag ctctatcggc tgatctggca gcggaccttg gcaagtcagg ccgcgagcgc
     1561 cgagatcgag cgcacgaccg ccgatatcga cgtcgccggc aaggacggca aacgctacgg
     1621 cgtgagagcc accggctcgg tcatccgctt cgacggtttc ctgaagatct acgaggaagg
     1681 gatcgacgac gccatgggcg aggacggcgc acttcccgcg ctcgccaagg gtgacgcgct
     1741 cgatccgcgc aagatcgagg cgaagcagca tttcaccgaa ccgccgcccc gctacagcga
     1801 ggcgaccctc atcaagaaga tggaggagct cggcatcggc aggccgtcca cctatgcctc
     1861 gacgctcaac gtgctccgcg accgcgaata tgtccgcctc gacaagaagc ggctctaccc
     1921 cgaggacaag gggcgcctgg tcacggcctt cctcgagagt ttcttcagcc gctatgtcga
     1981 gtacgacttc accgccgatc tcgaggagaa gctcgacctc atctccgccg gcgagatcga
     2041 atggaaggat gtgctacgcg acttctggcg cgagttcatc gcggcggtca acgacatcgg
     2101 cgacttgagg atcgcagagg tgctcgatgc gctgaacgac ctcctcggcc cgcacatctt
     2161 ccccgaccag ggcgcgggcg ccgaccccag agcctgcccc tcatgcgcgt cgggccgtct
     2221 cagcctcaag gtcggcaagt tcggcgcgtt catcggctgc tcgaattacc cagactgccg
     2281 ttacacgcgg cagctcgccg acaccaatgg cgagatggcc tctcttaacg aagggaaatt
     2341 gctcggcacc gatcccgaga ccggcctcgg catcacgctc cgcaccggaa ggttcggacc
     2401 ttacgttcag ctcggcgagc atgatggcgg ccgcggcgaa aagccgaagc gcgcctcgat
     2461 ccccaagggc aacgacccgc aagaggtcga tctcgaccgg gcgatcgcgc tcctctccct
     2521 cccgcgcgat gtgggattgc acccggagtc gggcaagtcg atcacggcgg gcttcgggcg
     2581 cttcggaccc tatatccagc acgacggcaa atatgccagc ctcggcggcc ccgaggaggt
     2641 gttcgagatc ggcctcaacc gcgccattag ccttctcgcc gagaaggcgg catcctcacg
     2701 cgcccgccgc ggcgcgaatg tcatcaggga tctcggcgcg cacccggcgc ttgggggcaa
     2761 ggtgcaagtc ctgaccggcc gctacgggcc ttacgtcaag cacggcaagg tcaatgcgac
     2821 gctgccgcgg gatcgcgagc cggacgaggt cacgctcgag gaggcggtcg ccttgatcgc
     2881 cgccaagagc gcaaagggcg cttccaagaa aacgactccc aagaagccgg cccgcaaggc
     2941 cggaaagagc aataaggagg ccgtcgagga gacggcgggc tgagcggcac tggtggcgaa
     3001 actcaagggg gcgccgcgca agaagacggc aggcaagcta ccttcgcggc aggacattct
     3061 cgattttctg gcgacgtcga gcagcgcggc cggcaagcgc gagatcgcgc gcgcctttgg
     3121 cgtcaagggc ggcgaccgga tcgcgttgaa aaacatgctg cgccagatgg ccgatgacgg
     3181 gctgatcgcg ggctcgcgcc gcaaattgat gcggcccggg gtgctgccgc ccgtgacggt
     3241 gatcgaggtg acggcccgcg accgcgacgg cgagtttatc gcccgccccg cggtgtggga
     3301 tgacgcgcaa ggtccggccc cgcgcatcct catggtcgag ggcaagcgcg aggcggatcc
     3361 ggtcgcgggg atcggcgagc gcgtgctcgc gcgcatcacg gcgcgctccg gcgatgccga
     3421 ttacccgtac caggcgcgga cgatcaagcg gctggcgcgc gccgcgcgaa gcctgctcgg
     3481 catcttccgc gcgctccccg gcggcacggg cgtaatcgat cccgtcgacc gcaagcagct
     3541 caaggaatgg ccggtgccgc gcggcgccac gggcgacgcg gcacatggcg aattggtgcg
     3601 cttcgagatc gcgcgcgcgt cgcgcatggg cgtgcagtcg gcccgcgtga ccgagcttct
     3661 cggcaatccc gaggcggagc agatgacgag cctcatcgcc gtccatgcac acggcattcc
     3721 cgatcgcttt ccagaagcgg cgcttgccga agcagcctcc gccatggagc cggatctgaa
     3781 gcgccgggag gatctccggc acttgcctct cgtcaccatc gacccgtcgg acgcgcgcga
     3841 ccatgacgat gcggtgtggg cggaagccga tcccaacccc gcaaatcgcg gcggctgggt
     3901 ggtgatcgtc gccatcgccg acgtcgcgtc ttacgtgcgg tcgggaagcg cgctggacaa
     3961 ggaagcgcgg aagcgcggca actcggtcta cttccccgac cgcgtcgtgc cgatgctgcc
     4021 cgagcggatc tccaacgatc tctgttctct cgtcgcgcac gagccaagac cctgtcttgc
     4081 ggtccgcatg gtgttcgaca aggacggaac gaagatcggg caccgcttct tgcgtgcggt
     4141 gatgcgatcg gccgcgagcc tgacctacga gcaggcgcaa gcggcaatcg atggacggct
     4201 cgacgagatg accgcgcccc tgctcgaaac cgtgctcagg ccgctgtggg gggcctatgc
     4261 cagcctcaac ttcgcgcgca ccgggcgcgg gccgctggag ctcgatctgc ccgaacggaa
     4321 gattctgctt gatgacgcgg gccgcgtgcg cggtgtcgcc acccctcccc ggctcgatgc
     4381 ccatcgtctg atcgaagagt tcatgatcgc cgccaatgtc gccgcggccg aaactctgga
     4441 ggccaagcga tcgccgctca tctatcgtgt ccatgacagc ccatcgaagg agaagctggc
     4501 gtcgctcggt gagtttctgg cgaccatcgg catcaagttg ccgaaatcgg gcacgctcaa
     4561 gcccatccac ttcaaccgcg ttctgaccga gacgcgcgcc acgcccaccg cggagctcga
     4621 tggcgaggtc attctgcgca gacaagcgca agccgagaac gccgtcggcc atttcggcca
     4681 attcggcttg aacc
//
LOCUS       DASUQP010000096         8084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1677426, whole genome shotgun sequence.
ACCESSION   DASUQP010000096 DASUQP010000000
VERSION     DASUQP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677426"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..263)
                     /locus_tag="VFY74_04230"
     CDS             complement(114..263)
                     /locus_tag="VFY74_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930949.1"
                     /translation="MPASNRWRRRAAINIVAGTYGRNVAIATGEWAAAIDRKLIELRR
                     AKKQC"
     gene            complement(557..1552)
                     /gene="hemH"
                     /locus_tag="VFY74_04235"
     CDS             complement(557..1552)
                     /gene="hemH"
                     /locus_tag="VFY74_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5930950.1"
                     /translation="MSKRERRVGVLLIALGTPDGTSYWPMRRYLNEFLSDRRVIEANR
                     ALWWFILNCIILVKRPVASGARYRSIWNEERNESPLRTITRSQAEKVSEILGPRGIKV
                     DWAMRYGNPSIAARIDALMAENCDRLLAFPLYPQYSASTTATANDKVFDALKALRAQP
                     ALRTVPPYPRDPAYIAALADSIRIHLRTLDFEPEAILASFHGIPQDYVDKGDPYQSHC
                     VETTEALREALGPLGEKVKLSFQSRFGRAEWLKPYTDKTVEGLAQAGVKRLAIITPGF
                     SADCLETLEEIAEENREIFLANGGERFSYIPCLNDSKGGIELLVHLIERELEGWV"
     gene            complement(1739..2152)
                     /locus_tag="VFY74_04240"
     CDS             complement(1739..2152)
                     /locus_tag="VFY74_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5930951.1"
                     /translation="MALPKTPALVTDCVIRDDEGRIVLVRRKSPPFKGAYALPGGFVD
                     IGETVEAACRREVLEETGLAIGTLRIVGIYSDPQRDPRGHTVSIAYTTLVAGTKRPRA
                     GSDADAAEWVADWRSLDLAFDHAQIVADAEKIELR"
     gene            complement(2176..2838)
                     /locus_tag="VFY74_04245"
     CDS             complement(2176..2838)
                     /locus_tag="VFY74_04245"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5930952.1"
                     /translation="MTLQLGETAPDFEADTTQGRIRFHEWIGDSWAVLFSHPKDFTPV
                     CTTELGYMARIKPEFDRRGVKIIGLSVDPVDRHSKWADDIKETQGFAPNYPMIGDPDL
                     KISKLYGMLPAAASGNAEARTPADNQTVRNVFVIGPDKKIKLILVYPMTTGRNFDEVL
                     RVIDSMQLTAKHRVATPVNWKDGEEVIIAGSVSDEEAKKIYPNGWKAPRPYLRIVPQP
                     RR"
     gene            complement(3129..3311)
                     /locus_tag="VFY74_04250"
     CDS             complement(3129..3311)
                     /locus_tag="VFY74_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX5930953.1"
                     /translation="MRTLLARFLKDESGSTAIEYTLIAAGIAIAILTAVNALGGSLAG
                     LFETIEGKIGAGAGGG"
     gene            4059..4718
                     /locus_tag="VFY74_04255"
     CDS             4059..4718
                     /locus_tag="VFY74_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930954.1"
                     /translation="MSKLVRGGLHEIKPEAYGDRPSALAFALAVIAQQAAAQGMRRDL
                     VLWCLTRHAAREWGRPYGPGLIASGLDPALLLIVLARNEDDAAWALEEGLKSRALIAA
                     LAATEIKTPLMARRLGLAAQGSCTPCLLLSDHRNANLPGTVTTWRIAARRSSVSFDAM
                     APGSPAWQLTLKRCRGEAPGRSFVVEFSHESFRLRLSAASSDRAAEAGESIERRGASS
                     G"
     gene            4624..6432
                     /locus_tag="VFY74_04260"
     CDS             4624..6432
                     /locus_tag="VFY74_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX5930955.1"
                     /translation="MSRFVCVFLPHLPIERLKRERASSGEAPLPADRPLALIGNEERG
                     LFLTALNEAALSEGLSAGLPLADARAICPRLLTLPAATKKDEAALRDLARWASCRYSP
                     TLNIDGADGLWLDVSGVPHLFGGERALLADLRRRLARAGFTARLALAETLGGAHALSR
                     FAHQSSVIVSDGEIGRALAPLPVEALRLAPEITRLLRRLGLKRIGQLYDLPRASLERR
                     FHAKETAEAVLLRLDQALSRREEPRILLLPQPDFAARLPLLEPLITPDGVLAGIDSLA
                     QALCGKLARAEQGCRRLALWVARADGSSTVIEAGLSAPSREPTHLLRLLKDRIETLDL
                     GFGVDFMSLQALVTETLPPAQTALTEANGKVRSETLIDRLVNRLGAHGVRRLGPRASH
                     LPELAQSLKSAFAGPSLWPEPASRKPMRPPLLFATPEPLTVLAEIPEGPPARFTWRRV
                     TRRVVKSEGPERIAPEWWRPLGVHNVTPHPPTACGGGPPLPQGERDFGGKPTNFNPSP
                     LVGEGDVPDFKSGTPGEGAVAQKQSGAPTSPPGGGLECVAPTRGRDESAANQARPRDY
                     YRIEDEDGHRYWVFREGLYQESELGPPCWYLHGVFG"
     gene            6443..>8084
                     /locus_tag="VFY74_04265"
     CDS             6443..>8084
                     /locus_tag="VFY74_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5930956.1"
                     /translation="MTSYAELEVTTNFTFLTGGSHPEELVATAKALGLAAIAVTDCNT
                     LAGAVRAHLAAKEAGIKFIVGSRLDLKDAPSLLAYPTDRAAYGRLCRLLTLGQRRAEK
                     GQCTLFLDDVAAHAEGLIFIVLPPQNYSPHPPTACSGGPLPLPQGERGRRGAASPSPS
                     PLAGEGGDPGFQSGAPGEGEHAKDFESELRRIKQALGVSARVYFAARHSYRGNDRARI
                     AALAALGRRVGVPLVATNAVLYHAPHRRALQDVLTCIREKCTIGEAGFRLKANAERHI
                     KSAKEMARLFAGHEDALRRSVEIARACAFSLDELKYEYPDEPVPDGKTPQSHLEDLTW
                     EGAAWRYPNGLPEKVRDTLHKELALIAELNYAPYFLTVHDIVRYARSQGILCQGRGSA
                     ANSAVCYCLAITNVDPTEIDLLFERFVAPERKEPPDIDVDFEHERREEVIQYIYARYG
                     RDRAGLAATVICYRGRLAVREVGKALGLSADTVAALATTIWGLSNSSLPEEYVRQAGL
                     DPSDPLLASVLELTQELIGFPRHLSQHVGGFVLTRGPLS"
BASE COUNT         1386 a         2722 c         2477 g         1499 t
ORIGIN      
        1 gtcacatagg cgaaggctcc cgcaccgccg gccgcattgt ccgtctcggc gatgacaggc
       61 tgcgtaacga gcagcccgat cagcaaggca atggcgaaag ccgcgcgcat ggttcaacac
      121 tgcttcttgg cgcgccggag ctcgatcagc ttgcggtcga tcgcagccgc ccattcgccg
      181 gtggcgatgg cgacgttgcg gccataggtg ccggccacga tgttgatcgc ggcgcgccgg
      241 cgccaccgat tggatgccgg caagaagcct ctggttgttc tccgccttct ctttctcgag
      301 cgctgcgatc tgcttgtcga tcttcgcgca gttcggcctg accgccttga tgtcgcgcat
      361 ggtcatcgcc gaggcgcaac cggccaggat caggatcgcg caagacagaa ccaatgcccg
      421 cgctcgcatc gccccccctc acgtcatcgc ccggcttgcc tgccttcgcc gaagcgaagc
      481 ttggcttcgc gcaggcagga ccgggcgacc ctactggatg cccgctttcg cgggcatgac
      541 aaagtcaaag cagcacctac acccagcctt caagctcccg ctcgatcaga tggacgagca
      601 actcgatccc gcctttgctg tcgttgaggc aaggaatgta gctgaaccgc tcgcccccat
      661 tggccaggaa tatctcccgg ttctcctcgg cgatctcctc gagcgtctcg agacaatcgg
      721 ccgaaaagcc cggcgttatg atggcaaggc gtttcacgcc cgcttgcgcg agcccttcca
      781 ccgtcttgtc ggtgtaaggc ttgagccact ccgcgcgtcc gaagcgcgac tggaagctga
      841 gcttgacttt ctcgccaagc ggtccgagcg cctcgcggag cgcctcggtc gtctcgacgc
      901 aatgactctg atagggatcg cccttgtcga cgtaatcctg cgggatgccg tggaacgagg
      961 cgaggatcgc ctcgggctcg aagtcgagcg tgcggagatg gattctgatc gagtcggcga
     1021 gcgcggcgat gtacgcaggg tcgcgggggt aaggcggcac ggtgcggagc gcgggctgcg
     1081 ccctgagtgc cttcagcgca tcaaacacct tgtcgttcgc ggtcgccgtc gtcgaagcgg
     1141 aatattgcgg atagagcggg aaggcgagca gccggtcgca attttccgcc atcagcgcgt
     1201 cgatgcgtgc ggcgatgctc ggattgccgt agcgcatggc ccagtcgacc ttgattcccc
     1261 ttgggcccag gatttcgctc actttctccg cctgacttcg cgtaatggtg cgaagcggcg
     1321 actcgttgcg ttcctcgttc caaatcgagc gatacctcgc tccgctcgcg acaggccgct
     1381 tcaccaggat gatgcagttc aagatgaacc accacagcgc ccggttcgcc tcgatcacgc
     1441 gccggtcgga aagaaactcg ttgaggtagc ggcgcatggg ccagtaggag gtcccgtccg
     1501 gcgtgccaag agcgatcagg agcacgccga cccgccgctc gcgctttgac acggacgctg
     1561 acttcgacga ctccgattgg cccgccaaac cgccgggacg aggcgcgtca ttcatcaatt
     1621 tcgctccgct cgagggtctt aagacccgtc gatagagcct ccccgacggg ccgcctagcc
     1681 tccagaccgg gcttccaaag ctttggcgcc acgtcccggc cttggcgctc ccgcaaccct
     1741 accgcagttc gatcttttcc gcatcggcaa cgatctgcgc gtgatcgaaa gcgaggtcga
     1801 ggctgcgcca gtccgcaacc cactccgcgg cgtcagcatc ggatccggcc cttgggcgct
     1861 tcgttcccgc cacaagcgtc gtataggcga tgctcacagt gtgtccgcgc ggatcgcgct
     1921 ggggatccga gtaaatgccg acgatgcgaa gtgtgccgat cgcaagcccg gtttcttcga
     1981 gaacttcgcg acggcatgcc gcttcgaccg tctcgccgat atcgacgaac ccgccgggaa
     2041 gggcgtaggc gcctttgaac ggcgggctct tgcgccggac gagaacgatg cgcccttcat
     2101 cgtcgcggat cacgcagtcg gtcacgagcg cgggtgtttt cggtaatgcc atggatcgcc
     2161 ggaagatcgc cccggtcagc ggcgcggctg tggcacaatg cggagataag gccgcggcgc
     2221 cttccagcca ttgggataga tcttcttcgc ctcttcgtcg gaaaccgacc cagcgatgat
     2281 cacctcctcg ccgtctttcc agttcacagg tgtcgccacg cgatgcttag ccgtgagctg
     2341 catggaatcg atcacgcgca gaacctcgtc gaagttgcgc ccggtggtca tgggatagac
     2401 gagaatgagc ttgatcttct tatcaggccc gatgacgaac acattgcgga cggtctggtt
     2461 atcggcgggc gttcgcgcct ccgcgttgcc cgacgcggcc gccggcagca tgccgtaaag
     2521 cttggagatc ttgaggtcgg gatcgccgat catggggtag ttcggagcaa agccttgcgt
     2581 ctccttgata tcgtccgccc actttgaatg gcggtccacg ggatcgacgc tcaagccgat
     2641 gatcttcacc ccgcgccggt caaactccgg cttgatcctc gccatgtagc cgagctcggt
     2701 ggtgcagacc ggcgtgaagt ccttgggatg ggagaacaac acagcccagg aatcgccgat
     2761 ccattcatgg aagcggatgc ggccttgcgt cgtgtccgcc tcgaagtctg gtgcggtttc
     2821 gccaagttgg agtgtcatgt taggtctcct cgtcggtagc gggcctcgag cctcgctgcc
     2881 cccgccccag acatttggga tgcgcggccc ggccagccaa tcgatccggc cgaaaaagaa
     2941 catcataccc tcgcgagagc aagcttcagc gcagaatttc tagcggcttt cgccgcggcg
     3001 cgccgcggcg cgacgaatcg caaaaaggga cgctcgccac gcggcggcaa gacctcatgc
     3061 tcctgtgaga aaggaaagcg acgtctgatc cgagcccggg acccggagca cttgcatgcg
     3121 atggggagtc agccgccgcc ggcaccggca ccgatcttgc cctcgatggt ctcgaacaag
     3181 ccggcgagcg agccgccaag ggcgttcacg gcggtcagga tggcaatggc gatgccggcg
     3241 gcgatcaagg tgtattcgat agccgtcgat cccgactcgt ccttgaggaa gcgcgcaagc
     3301 aaggtcctca ttttcggctc tcccgttaat tgctttgtcg gttatccccg gcctcggcta
     3361 cctgttgcgg cgtggccgga cccggcgcga gagctaacac cgccgcgtta aaactcacgt
     3421 taaagcaatc caacagaggc gagcggctta tggtgaatca agtgtaacaa atgcaaggac
     3481 tgaagcctaa aaaatcaagg atatgtgagg aaaatgactc gaaagctgtt ttcgattttt
     3541 accgaatgtt tacgactttg gcaaaaggcg gggctctaat cgtaaacaac gaggtggcag
     3601 tactacctgg gacctgcttg ggaccaccag ccttgactct gggtccgatt ccgtttagaa
     3661 caaaacatga acgagagtcg aggtgaggta gcgtgcgtag cgatgttcca ggcggtgcag
     3721 aaggtctcct cgttccgccg gctggcgttg caaaagcaga cggggcacgg gcgagggtcc
     3781 tgattgagca tctacgcgcg caagttcgcg cgctcgagca ggttcccgtg tctctggcca
     3841 atccccccgc actgggcgcg gcgcctcccc accccgcctg ctctttttct ccctccttcg
     3901 tcattgccgg gcctgacccg gcaatccacg ccaagagcct ctccccacgc cccgagccaa
     3961 cggcatggat gcccgaatcg caggctcgcc tgcgtcgtcc gggcatgacg agctccaccc
     4021 ccctccctcc tccccttcaa gggggcagga gttgtctttt gagcaaactc gtcagaggcg
     4081 ggcttcacga gatcaagccg gaagcttacg gcgataggcc ttcggcgctc gctttcgccc
     4141 ttgccgtcat cgctcagcaa gcggcggcgc aagggatgcg ccgggatctc gtgctgtggt
     4201 gcctgaccag acatgctgcg cgggaatggg gacgccccta tgggcctggc ctcatcgcct
     4261 caggcctcga ccccgctctt cttctcatcg tgctggcccg gaatgaagac gatgcggcct
     4321 gggctctgga agaaggtctg aagagtcgcg cgctgatcgc agcacttgcc gcgaccgaaa
     4381 tcaaaacgcc gctcatggcg cgccgcctcg gtctagccgc tcaaggctcg tgcacgccgt
     4441 gcctcctgct ttcggatcat cggaacgcaa accttcccgg aaccgtcaca acttggcgca
     4501 tcgccgcgcg aagaagctcc gtctccttcg acgcgatggc gccaggaagc cccgcttggc
     4561 agctcactct caagcgttgc cgtggcgaag cgccggggcg gagttttgtc gtggagttca
     4621 gtcatgagtc gtttcgtttg cgtctttctg ccgcatcttc cgatcgagcg gctgaagcgg
     4681 gagagagcat cgagcggcga ggcgcctctt ccggctgacc ggcccttggc gctgatcggc
     4741 aacgaagagc gggggctttt cctcaccgcg ctcaatgaag ctgccctgag cgaaggtcta
     4801 tccgccggtc ttcctcttgc cgatgcccgc gccatctgcc cgcgccttct cacgctgccc
     4861 gcagcgacca agaaggatga ggcagccctt cgcgatctcg cccgctgggc atcttgccgc
     4921 tatagcccca cgctgaatat cgacggcgcc gacgggcttt ggctcgatgt gagcggcgtg
     4981 ccccatctct tcgggggcga aagggcactt ctcgccgatc tcagacggcg gctcgcccgc
     5041 gcagggttca cggcgcggct tgctcttgcc gagaccttgg gcggcgcgca tgcgctttcc
     5101 cgtttcgcgc atcaatcttc cgtcatcgtt tccgatggag agatcgggcg cgcgctagcg
     5161 ccccttcccg tcgaggcctt gcgccttgcc cctgagatca cccgtctctt gcggcggctc
     5221 ggcttgaagc gcatcggcca gctctacgat ctgccgcgcg caagtctcga acggcggttc
     5281 cacgccaagg agacggccga ggccgtcctg ctccgtctcg atcaggcgct ttctcgccgc
     5341 gaggagccgc gcatcctgct tctgcctcag cccgatttcg cggcgcggct tccgctcctc
     5401 gagcctctca tcacccctga cggtgtgctg gccggcatcg acagtctcgc gcaggcactg
     5461 tgcggaaaac tcgcccgcgc cgagcaaggc tgccggcgtc ttgcgctttg ggtggcccgc
     5521 gccgacggct cctcgacggt gatcgaggcg ggcttgagcg ccccttcgcg cgagccaacg
     5581 catctcttgc gcctcctcaa ggacaggatc gagaccctcg acctcggctt cggcgtggac
     5641 ttcatgtcgc ttcaagcgct cgtcaccgag acgcttcctc ccgcgcaaac agctctgacc
     5701 gaagccaacg ggaaagtcag atccgagacc ctgatcgacc gcctggtcaa tcgcttggga
     5761 gcgcacgggg tgcgccgcct cggtccccgc gcaagtcatc ttcccgagct tgcgcaaagt
     5821 ctgaaaagcg cttttgccgg tccctcgctc tggcccgagc ctgcctcccg aaaaccgatg
     5881 cggccgccgc ttctcttcgc aacgcccgag cctctgacgg tgcttgccga aatccccgaa
     5941 gggccgccgg cgcgcttcac ctggcggcgc gtgacgcgcc gcgtggtcaa gtccgagggg
     6001 cctgagcgca tcgcgcccga atggtggcgt ccgctcgggg ttcataacgt tacccctcac
     6061 cctcccaccg cctgcggcgg cgggccacct ctcccgcaag gggagaggga ttttgggggc
     6121 aagcccacaa attttaatcc ctctcccctc gtgggagagg gtgacgtccc tgacttcaag
     6181 tcagggacgc cgggtgaggg agcggtagcg caaaaacaga gtggcgcgcc gacctcccca
     6241 ccagggggag gtttggagtg cgtcgctccc acgagaggaa gggacgaaag cgctgccaat
     6301 caggcaaggc cgcgcgacta ttaccgcatc gaggacgagg acggtcatcg ctactgggtg
     6361 ttccgcgagg gcctctatca ggagagcgag cttggaccgc cttgctggta cctgcatggc
     6421 gtcttcgggt gagcctttcg ccatgacaag ctacgccgag ctcgaggtca ccaccaactt
     6481 cacgttcctc accggcggct ctcatcccga ggagctggtg gcgacggcga aggccttggg
     6541 cctcgcggca atcgccgtca ccgactgcaa cacgcttgcc ggcgcggtgc gcgcccatct
     6601 cgccgccaag gaggccggca tcaagttcat cgtcggctca cgcctcgatc tgaaagatgc
     6661 gccaagcctt ctcgcctatc cgaccgaccg cgccgcttac ggccggcttt gccgccttct
     6721 gacgctcggc cagcgccgcg ccgagaaagg gcaatgcacg ctgtttctcg atgatgtcgc
     6781 ggcccatgcc gagggcctga tcttcatcgt cctccctccc cagaattatt cccctcaccc
     6841 tcccaccgct tgcagtggtg ggcccctccc tctcccacaa ggggagaggg gtcgccgtgg
     6901 agccgcaagt cccagcccct ctcccctcgc gggagagggt ggcgaccccg gctttcagtc
     6961 gggggcgccg ggtgaggggg agcacgccaa ggacttcgag agcgagctac ggcgcatcaa
     7021 gcaggcgctt ggggtcagcg cgcgcgtcta ttttgcggcg cggcattcct atcgcggcaa
     7081 cgatcgcgcg cgcatcgccg cgctggccgc gctcggcagg cgcgttggcg ttcccctcgt
     7141 cgccaccaat gcggtgctct atcacgcccc ccaccgccgc gcgctccagg acgtgctcac
     7201 ctgcatccgc gagaaatgca ccatcggaga ggccggcttc cgcctcaagg ccaatgccga
     7261 gcggcatatc aaatcggcga aggagatggc gcggctcttc gccggccatg aggatgcgct
     7321 ccgccgcagc gtcgagatcg cgcgagcttg cgccttctcg ctcgacgagc tgaaatacga
     7381 atatcccgac gagccggtgc ccgacggcaa gacgccccaa tcccatctcg aagatctgac
     7441 ctgggagggt gcggcctggc gctatccaaa cggtctccct gagaaagtgc gcgacacgct
     7501 ccacaaggag ctcgcgctga tcgccgagct taactacgcg ccctatttcc tcaccgtcca
     7561 cgacatcgtc cgctatgcgc gaagccaggg catcctctgc cagggaagag gctccgccgc
     7621 caactccgcc gtctgctact gcctcgccat caccaatgtc gatcccaccg agatcgacct
     7681 cctgttcgag cgcttcgtcg cgcccgagcg gaaggagccg cccgatatcg acgtcgattt
     7741 cgagcatgag cggcgcgagg aggtgatcca atatatctac gcccgctacg gccgcgaccg
     7801 cgccgggctt gccgccaccg tcatctgcta tcgcggacgg cttgccgtgc gcgaggtggg
     7861 caaggcgctg ggcttgagcg cggataccgt cgcagccctt gccaccacca tctggggctt
     7921 gagcaattca agcctgcccg aggaatatgt ccgccaagcc gggctcgatc cttcagatcc
     7981 gctgctcgcc agcgtgctcg agctcaccca ggagctgatc ggttttcccc gccatctctc
     8041 ccagcatgtc ggcggcttcg tgctgacgcg cggacccctc tccg
//
LOCUS       DASUQP010000097         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1132197, whole genome shotgun sequence.
ACCESSION   DASUQP010000097 DASUQP010000000
VERSION     DASUQP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132197"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFY74_04270"
     CDS             complement(<1..549)
                     /locus_tag="VFY74_04270"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5930957.1"
                     /translation="MMRLNTLIILAAGIAAGAAGSYWYAHRTETVTTPASAEQSGPAS
                     SKRKILYYRNPMGHPDTSPVPKKDPMGMDYIAVYADDEDDPNTVKVSLDKIQRSGVRT
                     EKVATRPLTRTVRGIGTVEHDESLLWIVTVRSDGYVEDLFVNKTGQHVREGEPLFRFY
                     SPQIQLAQADLVVAMRAEGASAK"
     gene            complement(1168..1629)
                     /locus_tag="VFY74_04275"
     CDS             complement(1168..1629)
                     /locus_tag="VFY74_04275"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX5930958.1"
                     /translation="MTEGVANPMKKAKLWYVIADGGRARFVTRDDKGAFRTVSSFVST
                     ELHARASDLGLDRPARVKESASPARHAIEPRRDLKEAAKEDFIKVVAEQLDAEHGRGQ
                     FDKLVLVAPPGVLTELKKALAKPLAKIVASDLQKDLTKIPDHDLTEHLALR"
     gene            1761..2030
                     /locus_tag="VFY74_04280"
     CDS             1761..2030
                     /locus_tag="VFY74_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930959.1"
                     /translation="MAVKDADMPKHHTLGEEVDAIAKHLASLRKDIEGLAGSIAQAGS
                     HQAERAQDAASEALSALETAVRRNPTQALGIALGIGFLVGVVLRR"
     gene            2174..2578
                     /locus_tag="VFY74_04285"
     CDS             2174..2578
                     /locus_tag="VFY74_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5930960.1"
                     /translation="MMHKGVEYVAPNAKLDVIAKKMQDHDVGAIPVCENGKTIGMVTD
                     RDIAIRALANGKDISTLEARDVMTRNVICCRDSDDAEDALRVMESNKIRRLPVLDDDD
                     KLVGMVSLGDISHALSRKLTGGVTKAVSAHHE"
     gene            2604..>2932
                     /locus_tag="VFY74_04290"
     CDS             2604..>2932
                     /locus_tag="VFY74_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX5930961.1"
                     /translation="MPIPLQIRFRNMDPSPAVEERIRKKAAKLERFHDRILSCTVVVE
                     APHRHHHQGKLYSVRVDISVPGKDVIVGRAKPIDHAHEDVYVAVRDAFVAVVRRLQDQ
                     TRKKRGSV"
BASE COUNT          568 a          894 c          949 g          521 t
ORIGIN      
        1 cttcgcggag gcgccttccg cgcgcatcgc cacgacgaga tcggcctgcg cgagctggat
       61 ttgcggactg tagaagcgga acagaggttc gccctcgcgg acatgctggc cggtcttgtt
      121 gacgaacaga tcctcgacgt agccgtcgga gcgcaccgtg acgatccaaa gcagcgactc
      181 gtcatgctcg acggtgccga tgccgcgaac ggtgcgggtg agcggccgcg tcgcgacctt
      241 ttcggtgcgc acgccagagc gctggatctt gtcgaggctg accttgaccg tgttgggatc
      301 gtcctcgtca tcggcgtaga cggcaatgta gtccatgccc atcgggtctt tcttcggcac
      361 cggcgaggtg tcgggatgcc ccatcggatt ccggtaatag agaatcttgc gcttgcttga
      421 ggcaggcccg gactgttcgg cggaggccgg cgttgtcact gtctcggtgc gatgcgcgta
      481 ccaatagctc ccggccgcgc cggccgcgat gccggcagcc agaatgatca gagtgttcag
      541 acgcatcatg acgcgtctcc cggaatcctt gttggtcggc ccccgccatg gagcggcaga
      601 ggatggcgtc tggctcacgc cgtgagcggg tgctcaaggc gcggcgacac gctttttgct
      661 caaggattca gacgcgggga ggcggcgaag ggggatttgg cgcgagcgat ccgcttgtcc
      721 cggtcacgag cgccaatctt ggcaacagat atgaaggatg gacgacgtcg atttcgccaa
      781 gagttaccgc tgaagcgctc gcgcatttta gggcgcatgc cgcgtccttg gtgcagtcgc
      841 gcttggcttg cttatcgcaa tcttggcccg gcgggcagca atccgcgtga gcggaaatcg
      901 gctcggtcga agacgcacga gcagacgcga cggcggccgg caacatggcc acggacaaag
      961 ccgtcacggc taccaagatg gcgcgggcga ttctcatgct tctccgcgat ttatcgaaaa
     1021 cactttccgt tcgccgtcaa gtgacagagc cgtgagtttg gaacccccga ccgccaaaga
     1081 agactaccac gacttggggc gacccggaaa tgccgggcgc taggccggcg cggccggcca
     1141 atcaagctac ccgaatcatg cccctctcta gcgcagggcg aggtgctctg tgaggtcgtg
     1201 gtccgggatc ttggtcagat ctttttgcag gtcgctggcg acgatcttgg caagcggctt
     1261 tgccaaggcc ttctttagct cggtcagaac gccgggtggg gcaaccagca cgagcttatc
     1321 gaattgcccg cggccatgct ccgcatcgag ctgctctgcc acgaccttga tgaagtcctc
     1381 cttggccgcc tccttgagat cgcggcgtgg ctcgatcgca tggcgcgccg gcgacgcgct
     1441 ctctttgacc cgcgccgggc ggtcaaggcc aaggtcgctc gcgcgcgcat gcagttcagt
     1501 cgagacaaag ctcgataccg tgcgaaaggc gcccttgtcg tcgcgcgtga cgaaacgtgc
     1561 cctgccgccg tcggcaatga cataccagag tttcgctttc ttcatgggat tggctactcc
     1621 ctcggtcacg cagactcctt tatatagaca gcgtatatag gcagcgaggt cacgaacttc
     1681 atctgcgtca aagcgatgac gccttgacga tccgcgataa agtatcccgc ttcgggaaga
     1741 gaccggctaa ggagagtgag atggccgtga aagacgccga tatgccaaaa caccacaccc
     1801 tcggcgaaga ggtcgacgcg attgccaagc atcttgcgtc cctgcgcaag gacatcgagg
     1861 gtctcgccgg ctcgatcgcg caggcgggga gccatcaggc cgagcgcgct caggatgcgg
     1921 cgagcgaagc cttgagcgcg ctcgagaccg ccgtccggcg caatccgacc caggcccttg
     1981 gcatcgctct tggcattggc ttcctggtgg gggtcgtatt gcggcgctag cgcgggccaa
     2041 ggcccgtccc ggacgccacg gtggatcaac accgggccgc ggggcctcaa tgaacctttg
     2101 tgccaacggc acgcattctg taacctgacc ggaggccgaa agtcaggagg tccgtatcat
     2161 gagagtgaga agcatgatgc acaagggcgt cgagtacgtc gccccgaacg cgaaactcga
     2221 tgtgatcgcc aagaagatgc aagaccacga tgtcggcgcc atcccggtct gcgagaacgg
     2281 caagaccatc ggcatggtga ccgatcgcga catcgccatc agggcgctcg ccaacggcaa
     2341 ggacatctcc acgctcgaag cgcgggacgt gatgacccgc aatgtcattt gctgccgtga
     2401 cagcgacgac gccgaggacg ctctgcgcgt catggagagc aacaagatcc gaaggctccc
     2461 cgtgctggac gacgacgaca agctcgtggg gatggtaagc ctcggcgaca tctcacatgc
     2521 gctatcgcgg aaactcaccg gcggggtgac gaaggcggtt tcagcccatc acgaataaac
     2581 gagaacgcgg aggttgtgcg ccgatgccga taccgttgca aatccgcttt cggaatatgg
     2641 acccctcccc cgccgtcgag gagcgcattc gcaagaaggc ggcgaagctc gaacgcttcc
     2701 atgaccgcat cctcagctgc accgtcgtgg tggaggcgcc ccaccgtcat catcatcagg
     2761 gcaagctcta cagcgtgcgg gtcgatatca gcgtgccggg gaaggacgtg atcgtgggcc
     2821 gggccaagcc catcgaccac gctcatgagg acgtgtatgt cgcggtgcgg gacgccttcg
     2881 tcgccgtggt acggcggctg caggatcaga cgcgcaagaa acgcggcagc gt
//
LOCUS       DASUQP010000098         7851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_588198,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000098 DASUQP010000000
VERSION     DASUQP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588198"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /gene="mltG"
                     /locus_tag="VFY74_04295"
     CDS             <1..1300
                     /gene="mltG"
                     /locus_tag="VFY74_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5930962.1"
                     /translation="DEPEPDPHPFLRMLDGLMTFVFLLACLGAGVFYWVKIKFDQPGP
                     LATSTVFAVPKGEGGSAVAERLEHEGIIADRRVFMTGILYFKYIKGQGSLKAGEYEIR
                     KHATMREVLDTVVEGKSIEHKVTLAEGLTSYQIVQKLMAHPKLRGKIAEIPPEGTLLP
                     DTYKFGRNDTRQDIIERMQAAQAKFLTKVWPTRDPDIVVTTPEEALILASIVEKETGR
                     ADERPRIARVFQNRLKKRMRLQSDPTIIYGLVGGKAVLDHPIQQEEVDRETAYNTYKI
                     NGLPPTPIANPGRAAIEAVLRPAKTNDHYFVADGTGGHVFAPTLDAHNKNVVEWRKIE
                     REIRAREAAEAAAKAAQEAAAGTAGAGAPVPEGAPAATPQDQAEPVAEGDSNATAADP
                     TVTSEILVDPSALGSTAHPFAMPEDAVEEAVPTPLRNPKR"
     gene            1389..2276
                     /locus_tag="VFY74_04300"
     CDS             1389..2276
                     /locus_tag="VFY74_04300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5930963.1"
                     /translation="MALNSMTGFARGDGVHGDTSWHWEARSVNGRTLDLRLRLPIGFE
                     GLEIRARGLAQEKLARGNCTINLWVKREAGRAEIRLNETALRQAEAVAERARDLTGLK
                     HARLDTLLGMRGVVETAEGEESEEAQAALSHAMLASLAATLDELVRARRGEGERLQAV
                     IEKQLVAVARLIDAAAKAAAQQPAAIAVRIREQIARLTDGTSTLDPERLHQEALLLAS
                     RADIQEELDRLRAHVGAAAELIASDQPVGRKFEFLAQEFNREANTVCSKAADIETTRT
                     GLDLKTVIDQLREQVQNIE"
     gene            2313..2918
                     /gene="gmk"
                     /locus_tag="VFY74_04305"
     CDS             2313..2918
                     /gene="gmk"
                     /locus_tag="VFY74_04305"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5930964.1"
                     /translation="MLVLSSPSGAGKTTLARRVIETEPEIAMSISHTTRAKRKGEEEG
                     VDYHFVDRDTFTRMRDHGAFLEWAVVFDNYYGTTRKPVEEALSQGRDVLFDVDWQGAA
                     SLRATAKDDVVTVFILPPTASELEQRLNVRAQDPPETVRRRMLGASNEIQHWQDYDYI
                     VINYDIAQSAAAVRSILAAERLRRSRLTGLKEFVQALQSEL"
     gene            complement(2915..3775)
                     /gene="rsmA"
                     /locus_tag="VFY74_04310"
     CDS             complement(2915..3775)
                     /gene="rsmA"
                     /locus_tag="VFY74_04310"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5930965.1"
                     /translation="MTTPDGLPPLREVIRALGLAAKRSLGQNFLLDFNLTRRIARAAG
                     PLEGVGVVEIGPGPGGLTRALLFEGAPRVVAIEKDARCLPALDAIAALYPGRLDVIAA
                     DAMELDYAALKLPSPARIVANLPYSVATPLLVSWLKSDPWPPWFDRMVLMFQREVAER
                     IVASPGGKDYGRLAVISQWRTSPRILFTLPAQAFTPRPKVDSALVEFVPKAKPQPSCD
                     VGSLERVTAAAFGQRRKMLRGALRRLVPDSETLLEAIGIDPKARAEELPVAAFCRLAN
                     ALAAARRSKP"
     gene            complement(3772..4794)
                     /gene="pdxA"
                     /locus_tag="VFY74_04315"
     CDS             complement(3772..4794)
                     /gene="pdxA"
                     /locus_tag="VFY74_04315"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5930966.1"
                     /translation="MASAERPLALTIGDPAGIGPDITLCAWSARAREAVPSFVLIGDP
                     AVLSARARALGLDVPIAELSAIEAGPSQFQRALPVLPIAGKGEIVAGRPDRCTAPLVT
                     QSIERAVGLLQQGAARAVVTNPIAKAVLYGAGFTFPGHTEYLAALASPEGQPLHPVMM
                     LAGPSLRVVPVTIHVPLKEVPGLLTRELIVKTIAIVAESLERYFGVKRPRLAVSGLNP
                     HAGEAGSLGREETETIAPAIEEARAMGLDVAGPYPADTMFHEAARATYDAAICMYHDQ
                     ALAPFKTLAFEEGVNVTLGLPFIRTSPDHGTAFDLAGTGKARPRSLIEALRMASAMST
                     RAEARA"
     gene            complement(4822..5838)
                     /locus_tag="VFY74_04320"
     CDS             complement(4822..5838)
                     /locus_tag="VFY74_04320"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX5930967.1"
                     /translation="MRSYHQRLRGIASVWIAAGGALPYRPGSVAVLLLAILAGGLVAV
                     GAQEVSIKILVNDDPISDYDIDQRERFLAITTQQQPSPALKKQAADMLIDERLQMQEG
                     RKASVTADEEDVTAILQDMAQKNNLNVEGLATALGKAGVNIKTLKDRIRAQLVWQQTI
                     QKKFRRDIQIADADVDKAMSEAGAEGGASEGGGAGEGGAAEPALQLRQVKFAIAAGSD
                     QRTIAAQLAAAESMRARFQSCAQLAKGVDGASVNTLQDHKTASLAQPARLLVMNAKVG
                     QMTPPTITQAAVELYAVCGKRSASGTNTAREQAQRQLMSQEMGLRAERRLRDVRAEAF
                     IEYR"
     gene            complement(5845..>7851)
                     /gene="lptD"
                     /locus_tag="VFY74_04325"
     CDS             complement(5845..>7851)
                     /gene="lptD"
                     /locus_tag="VFY74_04325"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX5930968.1"
                     /translation="AEGNVRIKDPDGAVITSDQITLTDDFRDGFIDALKLVTKDETRI
                     VASSASRQAGNVTVFEKGWFTPCKPCEENPNKPPTWRIRAGKITHKRDQATITYRNVV
                     FDFLGVPVAWVPWFQMADPTVKRKSGFLMPSYSHSNELGNTVQIPYYFALSDHYDFTF
                     APMVTEKAGTLLQGDWRHRLSSGAYRIQLAGVWDEGTFDSPTEGDFRGSIQTDGRFAL
                     NPYWSYGWDILGETDETFRRFYNLDSRLKTDRVSQIYLEGLHDRNYASMRFYNTQSLL
                     FTDEPFSDATVFPIIDYDYIVNRPILGGELSFNSNVMVFDNQEGISSNRLILEGNWRR
                     QMIDGIGQVYTPFARLRGDVYGVAGVDRGLNGSEEFATNETEGSSAILRGNAVGGIEY
                     RYPFVASTGNVTHVVEPIGQVIARPDTVGDQQEIPNEDALSLVFDDTLLFDIDKFSGY
                     DRIETGTRANVGVRYTAQLASGAYGRAVFGESYQIAGQNEFDTEFFRTTGLASDASDY
                     VGGLYIQAISNLGFSAQSRFDEQTFDIRRTDLASWASYGPARVKVNYADVTAEPGLAN
                     NEPREEIIVAGALAVTEAWSLLGNFRYDLATEQTITDGLGVRWQDDCFLMDVTYQRSF
                     IRDQDIEPDQRFLVNFSLKYLGSYQYSTSATSVFDASGSDSNDY"
BASE COUNT         1471 a         2428 c         2607 g         1345 t
ORIGIN      
        1 ggacgaaccc gagcccgatc cccacccgtt cttgcggatg ctcgacgggc tcatgacctt
       61 tgtcttcctg ctggcgtgcc tgggcgccgg cgtcttctat tgggtcaaaa tcaagttcga
      121 ccaacccggc cccctcgcca cgtcgaccgt cttcgccgtg cccaaaggcg aaggggggag
      181 tgccgtcgcc gagcgtctgg agcatgaagg aatcatcgcc gatcgccgcg tgttcatgac
      241 cggcatcttg tatttcaagt atatcaaggg gcaggggagc ctaaaggcgg gcgaatatga
      301 gatccgtaaa cacgcgacca tgcgagaggt gctcgatacg gtcgtcgagg ggaagtcgat
      361 cgagcacaag gtcacgctgg ccgaagggtt gaccagctac cagatcgtgc agaagctgat
      421 ggcccacccc aagcttcgcg gcaagatcgc cgaaataccg ccggaaggca ccctgctccc
      481 cgatacttat aagttcggcc gcaacgacac gcggcaagac attatcgaac gcatgcaggc
      541 cgcgcaggcc aagttcctca ccaaggtgtg gccgacgcgc gacccggata ttgtcgtcac
      601 gacgccggaa gaagcgctga tcctcgcctc catcgtggaa aaagaaaccg gacgggccga
      661 tgaacggccg cgcatcgcgc gcgtgttcca gaaccggctc aaaaaaagaa tgcggctaca
      721 gtccgacccc accatcatct acgggctcgt cggcggcaag gccgtgctcg accatcccat
      781 tcagcaggag gaagtcgacc gcgagacagc ttacaatact tataaaatca atggcttacc
      841 gcccacgccg atcgccaatc ccggccgcgc cgcgatcgag gcggtgctgc ggccggccaa
      901 gaccaacgat cactatttcg tcgccgacgg caccggcggc cacgtctttg cgccgacgct
      961 cgacgcgcat aataagaatg tcgtcgagtg gcggaagatc gagcgcgaga ttcgcgcgcg
     1021 cgaggccgcg gaggccgcgg ccaaggccgc tcaagaagcc gcggccggca ccgccggcgc
     1081 cggtgcgccc gtgccggaag gagcgcccgc ggccacgccg caagatcaag ccgaacctgt
     1141 cgccgaaggg gattcgaacg ccacagccgc cgatccgacc gttacgtcgg agatcctcgt
     1201 cgacccgtcg gcgctgggca gtaccgccca tccctttgca atgcctgagg acgctgtcga
     1261 ggaggcggtc cccacgccac tccgaaatcc gaagcgctag agggggcgca caagctgcct
     1321 ctcgcgcgcg actcgggcta taagcggcgt agtctggggt gcaggcaggc cggggggctt
     1381 acgatcggat ggcgctgaac agcatgaccg gctttgcccg tggcgacggc gtgcacggtg
     1441 ataccagctg gcactgggag gcgcgcagcg tcaacggccg cacgctcgat ctgcgtctgc
     1501 gtttgcccat aggtttcgag ggactcgaga tcagggcgcg cggccttgcc caggagaagc
     1561 tcgcgcgcgg caattgcacc atcaatcttt gggtgaagcg cgaagccggc cgcgcggaga
     1621 tcaggttgaa cgagaccgcg ctccggcagg ccgaggcggt ggcagaacgg gccagggatc
     1681 tcaccgggct taaacatgcc cgcctcgaca cgctgctcgg catgcgcggc gtggtggaaa
     1741 cggccgaggg ggaggagagc gaggaggcgc aagcggcgct cagccatgcc atgctcgcga
     1801 gccttgcggc gacgctcgat gagctcgtgc gcgcgcggcg tggcgagggc gagcggcttc
     1861 aagccgtgat cgaaaagcag cttgtagccg ttgcgagact gatcgatgcc gccgcaaaag
     1921 ccgcggcgca gcagcctgcg gcgattgcgg tacggattcg cgagcagatc gcccgcctca
     1981 ccgacggaac ctcgaccctc gatcccgaac gtctccacca ggaggctctg ctgttggcct
     2041 ctcgggccga catccaggaa gaactcgacc ggctgcgcgc ccacgttggg gcagcggccg
     2101 agcttattgc gagcgaccag ccggttgggc gcaagttcga gttcctcgcc caggagttca
     2161 atcgcgaggc caatacggtc tgctcgaagg ccgccgatat cgaaacgacg cggacggggc
     2221 tcgatctcaa gacggtcatc gaccagctgc gcgagcaggt ccagaacatc gagtgaggga
     2281 cgatggcgcg cggaatcgcg aggagggggg tcatgctggt tctgtcgtcg ccttccggcg
     2341 ccggcaagac cacgctggcg cgccgtgtga tcgagaccga gccggagatc gccatgtcga
     2401 tctcccatac cacccgggcg aaacgcaaag gcgaagagga gggcgtcgat taccacttcg
     2461 tcgaccgcga taccttcacg cgcatgcgcg accacggcgc gttcctggaa tgggcggttg
     2521 tgttcgacaa ttactacggc acgacgcgga agccggtcga agaggctttg tcacaagggc
     2581 gcgacgttct gttcgatgtc gattggcagg gtgcggcgtc cttgcgcgca acagccaagg
     2641 acgacgtcgt cacggttttc attcttcccc ccacggcgtc ggagctggag cagcgcctca
     2701 atgtgcgcgc gcaggatccg cctgagaccg tgcggcgcag gatgcttggc gcgagcaacg
     2761 agatccagca ttggcaagac tacgattata tcgtcatcaa ttacgacatc gcgcaatcgg
     2821 cggcggcggt gcgctcgatc ctcgcggcgg agcgactaag gcggtcgcgg ctgacgggcc
     2881 tcaaggagtt cgttcaggcc ctacaatccg agctctaagg cttgctccgc cgagcggccg
     2941 cgagcgcgtt ggcgaggcgg cagaacgcgg cgaccggcag ctcctcggcg cgcgccttgg
     3001 gatcgatgcc tatcgcttca aggagcgttt cgctgtcggg cacaagccgg cggagcgccc
     3061 cgcgcagcat cttgcgccgt tggccgaacg cggctgcagt gacgcgctcg aggctcccga
     3121 catcgcatga gggttgcggc ttggctttgg ggacgaactc gacgagggcc gagtcgacct
     3181 tgggccgcgg cgtgaaggcc tgggcgggga gcgtgaagag gatgcgcggc gaggtgcgcc
     3241 actgagagat cacggcaagg cgcccgtagt ccttgccgcc cggcgacgcg acgatccttt
     3301 ctgcaacctc gcgctgaaac atcagcacca tgcggtcgaa ccagggcggc cagggatcgc
     3361 tcttgagcca ggagacgagg agcggcgtcg cgacgctgta aggcaaattg gcgacgatgc
     3421 gggcagggga gggaagcttg agcgccgcgt aatcgagttc cattgcgtcg gcggcaataa
     3481 catcgagccg ccccggatag agcgcggcga tggcgtcgag cgccggcagg cagcgcgcgt
     3541 ccttctcgat ggcgaccacg cgcggggcgc cttcgaagag aagagcgcgc gtgagaccgc
     3601 cgggaccggg cccgatctcg acgacgccga ccccttcgag cgggcccgcg gcgcgggcga
     3661 tgcgccgggt caggttgaaa tcgagcagga agttctgccc gaggctcctc ttcgcagcca
     3721 agccgagcgc gcgaatgact tcgcgaaggg gcggcagtcc gtccggcgtg gtcacgcccg
     3781 cgcctcggcg cgcgtgctca tggcggaggc catgcgcagc gcttcgatga gactgcgcgg
     3841 gcgcgccttg ccggtgccgg caaggtcgaa tgccgtgccg tggtcggggg atgtgcggat
     3901 gaaggggagc ccgagggtca cgttgacgcc ttcctcgaat gcaagcgtct tgaacggcgc
     3961 gagcgcctga tcgtgataca tgcagattgc cgcgtcgtag gtcgcgcgcg ccgcctcatg
     4021 gaacattgta tcggcgggat agggaccggc gacgtcgagg cccatggccc gcgcctcctc
     4081 gatcgccggc gcaatggttt cggtctcctc gcggccgagg cttcccgcct ctccggcatg
     4141 gggattgagg ccggacacgg cgaggcgcgg acgcttcacg ccgaaatagc gctcaaggct
     4201 ttcggcgacg atggcgatgg tcttgacgat cagctcgcgc gtgagcaggc cggggacctc
     4261 tttgagagga acgtggatcg tgacagggac cacgcgcaag gagggaccgg cgagcatcat
     4321 gaccgggtgg agcggctgcc cctcgggcga ggcaagcgcg gcgagatatt ccgtgtgtcc
     4381 cgggaaagtg aagcccgcac cgtagagcac ggccttggcg atggggttgg tcacaacggc
     4441 gcgcgcggcg ccttgctgca ggaggcccac ggcccgctcg atcgattggg tgacgagagg
     4501 cgccgtgcag cgatcgggcc gtccggccac gatctcgcct ttgccagcaa tcgggagcac
     4561 cggcagagcg cgctgaaact ggctcgggcc cgcttcgatc gcggagagtt cggcaatcgg
     4621 cacgtcgagc ccgagcgccc gggcgcgcgc ggaaagcacg gcaggatccc cgatcaggac
     4681 gaacgagggg accgcctcgc gcgcgcgcgc agaccaggcg caaagcgtaa tgtcgggtcc
     4741 gatcccggcg ggatcgccaa ttgtgagcgc cagaggccgt tccgccgaag ccatgggctt
     4801 cggctagcat gaaacacgtc cctagcggta ctcgatgaac gcctccgcac gcacatcgcg
     4861 caaccgccgt tcggcgcgaa gccccatttc ctggctcatc aattgccgct gagcctgctc
     4921 gcgggcagta ttggtgcccg aggcggaacg cttgccgcag accgcgtaaa gctcgacggc
     4981 tgcctgcgtg atggtcgggg gcgtcatctg cccgaccttg gcgttcatga cgagaagccg
     5041 cgcgggctga gccagagagg ccgtcttgtg atcctgcagc gtgttcacgc tcgcgccatc
     5101 gacgcctttg gcgagctggg cgcaggattg aaaccgggcg cgcatgctct cggcggccgc
     5161 aagctgggcg gcaatggtcc tctggtcgga gcccgcggcg atcgcgaact tgacctggcg
     5221 caactgtagg gccggctctg ccgcgccacc ttccccagcc ccgcctccct cagacgcacc
     5281 tccctcagca cccgcctccg acatggcctt gtccacgtcg gcgtcggcga tctgaatatc
     5341 gcggcggaac ttcttctgta tcgtctgctg ccagacaagt tgcgcccgaa tacggtcctt
     5401 cagcgtcttg atgttgacgc cggccttgcc aagcgcggtc gcaaggccct cgacattgag
     5461 gttgttcttc tgcgccatgt cctgaaggat tgccgtcaca tcctcttcgt cggccgtcac
     5521 ggaggccttg cggccttcct gcatttgcaa ccgttcatcg atcagcatgt cggcggcctg
     5581 tttcttgaga gcgggcgagg gttgctgctg cgtggtaatg gcgagaaagc gttcgcgctg
     5641 atcgatgtcg tagtccgaga tcgggtcgtc gttgacgaga atcttgatcg atacttcctg
     5701 cgcgccgacg gccacgagac ctccggccag aatcgcaagc aggagcaccg ccaccgatcc
     5761 agggcggtag ggcaaggcac cgccggccgc aatccagacc gatgcgatac cccttagacg
     5821 ctgatggtaa ctccgcattg acctttagta gtcgttgctg tcagacccac ttgcgtcgaa
     5881 cacgctggtc gctgaggtcg agtattgata gctcccgaga tatttcagcg agaagtttac
     5941 caggaaacgc tggtccggct cgatgtcttg atcgcggatg aaagaacgct gataggtcac
     6001 atccatcagg aaacagtcgt cctgccaccg cacgcctaag ccgtccgtga tcgtttgctc
     6061 ggtcgcgagg tcgtaacgga agtttccgag caacgaccaa gcctcggtga ccgccagggc
     6121 gccggcgact atgatctcct cgcgcggctc gttatttgcg agtcccggtt cggcggtgac
     6181 atcggcatag ttcaccttta cccgcgccgg accgtagctc gcccatgagg caagatcggt
     6241 gcgccggatg tcgaaggtct gctcatcgaa gcgggactgc gccgagaagc cgagattaga
     6301 aatggcttga atgtaaagac cgccaacata atcggaggcg tcggaggcga ggccggtggt
     6361 acgaaagaat tcggtgtcga actcgttctg cccagcgatc tgatagctct cgccaaagac
     6421 ggcgcgccca tacgcgccgg aggccaactg ggcggtatag cgcacgccca cattggcgcg
     6481 tgtgccggtc tcgatccggt cgtagccgga gaatttgtcg atgtcgaaca gaagggtgtc
     6541 gtcgaacacc aggcttagag catcttcgtt cggaatttcc tgctgatcgc cgaccgtatc
     6601 gggccgggca atgacctggc cgatcggctc aactacgtgg gtgacgtttc cagtactggc
     6661 gacgaaagga tagcgatact cgatgccgcc tacggcattg ccgcgcagga tggcgctgct
     6721 cccttccgtc tcattagtgg cgaattcttc cgaaccgttc agccctctgt caactccggc
     6781 aacgccgtag acgtcgccgc gaagccgggc aaagggcgtg tagacctgac cgatgccgtc
     6841 gatcatctgg cgccgccaat ttccctccaa aatcagccgg ttggagctga tgccttcttg
     6901 gttgtcgaat accatgacgt tggagttgaa actcagttcg ccgccaagga tcggccgatt
     6961 gacgatgtag tcgtagtcga tgatcgggaa caccgtggcg tccgagaacg gctcgtcggt
     7021 gaacaacagg ctctgcgtgt tgtagaaccg catggaggcg taatttcggt cgtgcagacc
     7081 ttcgagatag atctgcgaaa cgcggtccgt cttgagccgg ctgtcgaggt tgtaaaaccg
     7141 ccggaaggtc tcatcggtct cgccgagaat atcccagccg taactccaat acgggttgag
     7201 cgcaaaccta ccgtcggtct ggatactgcc gcggaagtcc ccctcggtgg gactgtcgaa
     7261 ggtgccttca tcccaaacgc cggcaagttg aatacgatag gcaccactgg acagacggtg
     7321 ccgccaatcg ccctggagca gggtccccgc cttctcggtc accatcgggg cgaaggtgaa
     7381 atcgtaatgg tccgacaggg cgaagtagta ggggatctgc accgtattgc cgagctcgtt
     7441 ggaatggctg taactcggca tcaggaagcc ggacttgcgc ttcacggtcg ggtcggccat
     7501 ttggaaccag ggaacccaag ccaccggcac gcccaggaag tcgaagacca cattcctgta
     7561 ggtgatggtg gcctgatcac gcttatgcgt gatcttgccg gcacgaatgc gccaggtcgg
     7621 aggcttgttt ggattttcct cgcacggctt gcacggcgtg aaccaccctt tctcgaacac
     7681 cgtgacgttt ccggcctggc gcgaggccga ggaggcaacg atacgggtct cgtccttggt
     7741 aacgagcttc agcgcatcta tgaagccgtc gcggaagtca tcggtgagtg tgatttggtc
     7801 tgaggtgatc accgcgccgt cgggatcttt gatgcggaca ttgccctcgg c
//
LOCUS       DASUQP010000099         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1132702, whole genome shotgun sequence.
ACCESSION   DASUQP010000099 DASUQP010000000
VERSION     DASUQP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132702"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFY74_04330"
     CDS             <1..745
                     /locus_tag="VFY74_04330"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX5930969.1"
                     /translation="LVHEMRTRIVTSPVFGGDRILGAILFEGTMDRDIKGVPSPEYLW
                     KVKHVVPFLKIDEGLADAADGVQMMKPMPGLDQLLERAKAKGVFGTKARSVVKEANAK
                     GVAAIVRQQFDVAKQVLAHGLVPIIEPEIDIKCSDKAAAEKLLRKAILAALDELAPGQ
                     LVMLKLTLPEEDDFYRPCIEHPNVLKVVALSGGYSRDEANARLARNHGMVASFSRALV
                     EGLVAEQSAEEFNGALERSIASIFAASMT"
     gene            complement(963..2276)
                     /locus_tag="VFY74_04335"
                     /pseudo
     CDS             complement(963..2276)
                     /locus_tag="VFY74_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243213.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
     gene            complement(2380..2586)
                     /locus_tag="VFY74_04340"
                     /pseudo
     CDS             complement(2380..2586)
                     /locus_tag="VFY74_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          449 a          902 c          918 g          497 t
ORIGIN      
        1 cctggtccat gagatgcgaa cgcgcatcgt cacgagcccc gtgttcgggg gcgatcgcat
       61 cctcggagcg atcctgttcg agggcacgat ggaccgcgac atcaagggcg taccgagccc
      121 cgagtacctg tggaaggtca agcacgtggt cccgtttctc aagatcgacg aggggctcgc
      181 cgacgccgcc gacggcgtgc agatgatgaa gccgatgccc ggcctggatc agctcctcga
      241 gagggccaag gcgaagggtg tcttcgggac caaggcgcgc tcggtcgtga aggaagcaaa
      301 cgcgaagggc gtcgccgcca tcgtccgcca acagttcgat gtggcgaagc aggtcctcgc
      361 gcacggactc gtgccgatca ttgagcccga gattgacatc aagtgctccg acaaggcggc
      421 ggccgagaag ctcctgcgga aggcgatcct ggcggctctc gacgagctcg cgcccggcca
      481 gctcgtgatg ctcaagctca cgctccctga ggaggacgac ttctaccgtc cctgcatcga
      541 acaccccaac gtcctgaagg ttgtcgcgct ctccggtggc tactcacggg acgaggcgaa
      601 cgcccgtttg gcgcgcaacc acgggatggt cgccagcttc tcgcgggcgc tcgtcgaggg
      661 gcttgtcgct gagcagtccg ccgaagagtt caacggcgct ctggagcgct cgatcgcgag
      721 tatcttcgcg gcctcgatga cctgacgccc ggggccatca gtcggcgccc agctcgtaca
      781 cggtcgtgat gtcgatcgcg ctgtggatcc tccggtagac gaggcacctg tcggctgatg
      841 tccccagacc ggccgtcgaa acgctggaca tgggagatgc ggaacgcgtc gatatggccg
      901 ttgaagggat cggcgatgcc gctcacatgc tatcgaaggg cagcgaagcc ccacaatgcg
      961 ggctatctgc gcggcgggtt gtccatccta aaggcgtccg gacgtatccc gtgctcgact
     1021 tggcgccagc tcaccggggc cgcaatcggg aatccaggcc gcgcccgcgg cgaccaggca
     1081 ccgatcgccg ttgtgccgcg gccgttcctc agataatcga tgaagatgcg ccccgcgcgt
     1141 ttttctggcg ccgaggcgat cgtgttggtc cggtgccgct gcagcgaggc gccgcgcgat
     1201 ctgcttggcg tacactcgcg cctggtcgtg gtccatcgtt cggtcgagcg gcgccatcag
     1261 atgcaatccc ttgcccccgg tcagcttcgg ccacggctcc aggccctcat ccttcatcat
     1321 tcggcgcagc gtcagcgccg tctcgatcac gaatccccag gcaacgccct tgcccggatc
     1381 gagatcgaag acgagcatgt cgggatgctc tatgtcgtcg atgcttgcgt tccatgggtg
     1441 cagctcgacc gcgccgatct cgaccaggcc gagcaggccg gccaggtcat ccacccagag
     1501 ccgtgcgccc tcgccccctc ccgcttctcg atcgtgagcc tgtgaactgt ctccgggacc
     1561 ggcggcagcg tacccttgtg atagaatgtt gtgcctctcg tgtggcgcac gagcttgagc
     1621 ggccttcgcc cgacatgcgc gaaggcccgc ttccagacct tcctccagta cgcggcgagt
     1681 tcctctttcg atggcgccac ggcatccgga agaagttgca ggatgttctc gcgcggcacg
     1741 gcgatccgat ctcggcggcc ttgtgcgcgc gagggcgtgc cggcaggcgt gcgcgccggc
     1801 ggcgcttcgc gatcctcgcg caaccccttg aacacggctt cgcgcagcag ctggtgttca
     1861 gtcatggtgc tgtaggccac ttcggcctcc atgaccggct cgacccaggt cgccttcggc
     1921 ttcttgaccg gctcggagag cggggtgtct gtccgaatga acggatcgag ccgctcgcgc
     1981 agatcgcgcg cctccgcttc ggtgaagccg ccccgcacct tgccggcata gagcagccgg
     2041 tctccgtcac ggcgaccgat gtagaacgag gcgatcttgc gcggcttcgc cccgagcttc
     2101 tcgacgaagg ccacgatcgg aaacgcgtcg cgcttgccgc acttgacctt aatccagctt
     2161 tccacctggc cggagcgata cggcgcgtcc tgctgcttgc tgacgatgcc ttcgagaccc
     2221 atggcgcagg cgcgctctcg gatctcggcg ctgtccccct ccaggtgctc ggcgtagagg
     2281 tgtcaacggc ggagagaaaa tgcatcggcg tggcggcgca aagatgcatc aggtggcgtg
     2341 atgacaaggg ccccgcgcgg ggcccttgtc atttggcggt caggcgggct gcggttggct
     2401 ggcgtcggag gtgggcgagc gccggcggtg cttgctttgc cggagacgat agctgtcacc
     2461 gttcatctcg aggatgtgga cgtgatgggt gaggcggtcg aggagcgcac cggtcaggcg
     2521 ctcggagccg aacacgctgg tccactcgtc gaagggcaga ttgctggtga cgatgaccga
     2581 gccacgcaat cagacctcga acgccgacgc ttgcgcggct gatccttaaa caggtggtag
     2641 gtggcgagcg cggcgccgac ggagccgtcc gcatcacccg tggccggctg gacccagatc
     2701 cataacgtgt ggtctcgtcg ttgcgtgtcg tccgaactcc acgccagagg cagtttcaga
     2761 ttgact
//
LOCUS       DASUQP010000100        15119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_451665,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000100 DASUQP010000000
VERSION     DASUQP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15119
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451665"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..245)
                     /locus_tag="VFY74_04345"
     tRNA            complement(169..245)
                     /locus_tag="VFY74_04345"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Asp,seq:gtc)
     gene            1017..1268
                     /locus_tag="VFY74_04350"
     CDS             1017..1268
                     /locus_tag="VFY74_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930970.1"
                     /translation="MKYLGSALIGLLGTFVLTTTASAAIVCNDEGDCWKVKEKHKYPA
                     DVNIQVYEDDWRWPEGAKYRWRDAGPGRGYWRGGVWIDF"
     gene            complement(1288..1363)
                     /locus_tag="VFY74_04355"
     tRNA            complement(1288..1363)
                     /locus_tag="VFY74_04355"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1328..1330),aa:Val,seq:tac)
     gene            complement(1466..1741)
                     /locus_tag="VFY74_04360"
     CDS             complement(1466..1741)
                     /locus_tag="VFY74_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5930971.1"
                     /translation="MNKAELIAHVAKDSDLSKDAAEKAVDAMFRNIERALRGGDTVRI
                     VGFGNFQVAQRKASTGRNPRTGAEVHIPASRVPKFRAGKALKEACNS"
     gene            complement(1944..4373)
                     /gene="lon"
                     /locus_tag="VFY74_04365"
     CDS             complement(1944..4373)
                     /gene="lon"
                     /locus_tag="VFY74_04365"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5930972.1"
                     /translation="MTESKPSTVREGETELYPVLPLRDIVVFPYMIVPLFVGREKSIA
                     ALEEVMRSDKQILLVAQKNASDDEPSTDGIYTMGTLASVLQLLKLPDGTVKVLVEGTR
                     RAAIRRYTENESYFEAEVERVAETVGAEDEIEALARSVVSQFESYVKLNKKISPEVLG
                     TASQIEDYSKLADTIASHLAIKIAEKQQILEITSVSERLERVFTLMESEISVLQVERK
                     IRSRVKRQMEKTQREYYLNEQMKAIQKELGDNEEGRDDLAEIEQKIEKTKLSREAREK
                     ALGELKKLRQMSPMSAEATVVRNYLDWLLTIPWGVKGKIKRDLDEAQKILDEDHYGLE
                     KVKDRIVEYLAVQNRTNMLKGPILCLVGPPGVGKTSLGKSMARATGREFVRMSLGGVR
                     DEAEIRGHRRTYIGSMPGKVIQSMRKAKKINPLFLLDEVDKMGADFRGDPASALLEVL
                     DPEQNHTFNDHYLEVDYDLSNVMFVTTANTLNIPPALMDRMEIIRIAGYTEDEKTQIA
                     RRHLLPAMTKAHGLEAEEWGLDDDALKEIIRRYTREAGVRNLEREIAKIARKSVKELL
                     TTKKKSIRISLKNLEDYLGVQKFRYGEAELEDQVGVVTGLAWTEVGGELLTVEGVMMP
                     GKGKMTVTGNLRDVMKESIQAANAYVRSRAVDFGIEPPLFEKRDIHVHVPEGATPKDG
                     PSAGVAMVTAIISLMTGIPVRREVSMTGEITLRGRILPIGGLKEKLLAALRAGIKTVI
                     IPDENAKDLTEIPDELKNALDIRPVSRMEEVLKIAFTRPAQPIAWEEVAEAGAQVVPP
                     QDETQPGVTAH"
     gene            complement(4679..5944)
                     /gene="clpX"
                     /locus_tag="VFY74_04370"
     CDS             complement(4679..5944)
                     /gene="clpX"
                     /locus_tag="VFY74_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX5930973.1"
                     /translation="MSKVSGADSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREENKSSLVKSRDGVPTPMEICAVLDDYVIGQDYAKRVLSVAVHNHYKRLNHST
                     KNSDVELAKSNILLIGPTGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQAADYNVERAQRGIVYIDEVDKISRKSDNPSITRDVSGEGVQQALLKIMEGTI
                     ASVPPQGGRKHPQQEFLQVDTTNILFVCGGAFAGLEKIISARGRSTSIGFGAKVESVD
                     DRRTGDILKGVEPEDLLKFGLIPEFIGRVPVIATLDDLDEAALMRILTEPKNALVRQY
                     QRLFEMEDVQLSFAEEALRTISKKAIDRKTGARGLRSIMEGILLDTMFELPSLRGVEE
                     VVISSEVVDGKARPLRIYSDRHEELGTSA"
     gene            complement(6249..6899)
                     /gene="clpP"
                     /locus_tag="VFY74_04375"
     CDS             complement(6249..6899)
                     /gene="clpP"
                     /locus_tag="VFY74_04375"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX5930974.1"
                     /translation="MPDPADIYSSTLVPMVVEQTNRGERAYDIYSRLLKERIVFVTGP
                     IEDHVASVITAQLLFLEAENPKKEISMYINSPGGIVTSGMAIYDTMQFIRPAVATLCI
                     GQAASMGSLLLAAGEKGMRYALPNARILLHQPSGGFQGQASDIERHAEDIIKMKRRLN
                     EIYVRHTGQDYDTVDRTLDRDYFMSAEDARSFGLIDEVITKRHPLEEKVEKLAPVS"
     gene            complement(7001..8452)
                     /gene="tig"
                     /locus_tag="VFY74_04380"
     CDS             complement(7001..8452)
                     /gene="tig"
                     /locus_tag="VFY74_04380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5930975.1"
                     /translation="MDITETTTEGLRRQLKVVIGAEELERRLSARLDELKGKARLKGF
                     RPGHVPKEHLRKVFGRSVMAEVVQQAVAETSREALSQREERPAFQPTVALPQDAVEID
                     KIFAGRSDLAYTLSFEVLPQIELMDFGKLALDRPVAAVTAADIDKALERLRNANMRYK
                     AKDGAAESGDRLIIDFTGSIDGTPFEGGSTTDAPVVLGSGNFIPGFEEGLVGAHAGEE
                     REVDATFPVAYQEANLAGKAARFSVKVKEVGAPETPPLDDDFAKSLGVESVDKLRETI
                     KQRMENERGGVSRLKVKRALLDALSEGHSFELPPTLVDNEFNAIWTQVKADLERSKRS
                     FADEGTTEEKAEQEYRELAARRVRLGLILSEVGTRNKISVTDEEVSRALLERVRQFPG
                     QERKVYDHYRNNPELLAEFRAPIFEDKTVDFILELARVTEKKVSAEELYADPDEDGDH
                     AHHHHEHDHDHDHDHEHGHDHGKGPKKRGKKRK"
     gene            complement(8519..8603)
                     /locus_tag="VFY74_04385"
     tRNA            complement(8519..8603)
                     /locus_tag="VFY74_04385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8567..8569),aa:Leu,seq:tag)
     gene            8912..9460
                     /locus_tag="VFY74_04390"
     CDS             8912..9460
                     /locus_tag="VFY74_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX5930976.1"
                     /translation="MGRINKVQNEAEVISASGTEAALIGTQVRMKDELCTGADARLQV
                     TFRDNTVLTLGEQARVIIDRYVYDPERAIGETVLKAAAGAFRFAAGRMDSLKDKNILV
                     STPVAQIGVRGTEFWGGPIDGTYGVFLIEGEVTVTSQGGSVTLSGSGQGIDLPSALDP
                     PGPPSPWPAEKVARAVESVALH"
     gene            9618..10652
                     /locus_tag="VFY74_04395"
     CDS             9618..10652
                     /locus_tag="VFY74_04395"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5930977.1"
                     /translation="MTTRSGIIEAIGNTPLIKLKRASEETGCTILGKAEFMNPGQSVK
                     DRAALFIIRDALNAGKLQPGGTVVEGTAGNTGIGLALVGNALGLKTVIVIPDTQSDEK
                     KEMLRLTGAELIEVPAVPYKDPNNYVKYSGRLAEELSRSEPHGAVWANQFDNVANRQG
                     HIETTAEEIWNDLGGKVDGFICAVGTGGTLAGVSMGLKAHNPDVVIGLADPLGAALYN
                     YYKHGELKAEGSSITEGIGQGRITKNLEGAKIDEAYQIPDGEALPIIFDLLSEEGLCL
                     GGSSGINIAGAIRLARDLGPGHTIVTVLADYGTRYQSRLFNPKFLREKGLPVPGWLDQ
                     GQPACPRVFI"
     gene            10677..11531
                     /gene="sseA"
                     /locus_tag="VFY74_04400"
     CDS             10677..11531
                     /gene="sseA"
                     /locus_tag="VFY74_04400"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEX5930978.1"
                     /translation="MQKTKTWLTETEELEHELDAPDLVIIDASWHMPGEGAARAEYLA
                     EHVPGALFFDIDEIADTKSSLPHMLPPPEKFSSRMRSMGIGDGSRIVVYDSQGLYSAA
                     RVWWTFRVMGAEDVSVLNGGLPKWKREGRPLESGEPRTRTPRHFTARRNLDLVRDLSD
                     LKTLLKDRSTEIVDARSAERFAGTVPEPRPGLRSGHIPGARNLPYGRLLNKDGTLKSP
                     REIERLFEGVGVDLGKPVVTSCGSGITASVLALALAEIGHRKTAVYDGSWSEWGADQS
                     LPIETGKA"
     gene            11564..12733
                     /gene="metC"
                     /locus_tag="VFY74_04405"
     CDS             11564..12733
                     /gene="metC"
                     /locus_tag="VFY74_04405"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX5930979.1"
                     /translation="MSKRTRKQSDRTRLAHAGRAPSRQHGFVNMPVYRGSTVIFPTLA
                     CLEAEDQDFSYGRLGTPTVSALEEAIAELEGGYRTRLAPSGLSAIATALFAFVAAGDE
                     ILVSDSVYRPTRRFCDHVLKRLGVKTTFYDPLIGGAIERLITDKTRAVFAESPGSQTF
                     EVQDVPAIARAARKAGAVMILDNTWATPLYFKPFTHGVDVSIQAATKFIVGHADAMLG
                     TITATEQAFPAVFKTHEELGLCPGPEDVYLGLRGLRSLGVRLERHHKSALELARWLGE
                     RPEVERVIYPALPSDRGHALWRRDFTGAPGLFSIVLKPIPRAKLGAMLDGLSLFGMGY
                     SWGGFESLILPFDPRAYRTATKWEDKGQALRLHVGLEDVEDLKADLDAGFARLRG"
     gene            12770..13561
                     /locus_tag="VFY74_04410"
     CDS             12770..13561
                     /locus_tag="VFY74_04410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5930980.1"
                     /translation="MDFKLQGKRALVTGSTQGIGFAIAQGLAREGATVVVNGRDETKT
                     REAAKTIGAATKAPVTGIAADVGKAAEVDKLLKKLGPVDILINNAGIFEPTPFFDIAD
                     SEWLRFFEVNVMSGVRLSRALLPHMLKQNWGRIIFISSESGLNIPAEMIHYGMTKTAQ
                     LAVSRGLAEIVPGTGVTVNAVLPGPTLTEGVAGFLESLAKERGLSVKEMEVELIKSMR
                     PSSLIRRMATPEEVANPVVYLCSEAASATTGAAIRVDGGVVRTIG"
     gene            complement(13575..14456)
                     /locus_tag="VFY74_04415"
     CDS             complement(13575..14456)
                     /locus_tag="VFY74_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930981.1"
                     /translation="MRPVVVLLLASLTLCTSAAAFSTPAAAAPKLLWETKGLAQPESV
                     VQDPATGALYVSNIAGAVMQKDGNGFIAKLRPDGTVVTREWVKGLNAPTGLALHDRTL
                     YAADVDELLEINVASGEIVKRHAAKGAIFLNDVAVAQDGTVYVSDTPMNAIWRLKDGT
                     FEPWLANDALNGPNGLLVLGDKLIVASFGKLPDQGSPELGGLIAVDLKDQSVSKLRDD
                     AIGNLDGLQSLAPGIYLVTDWAAGGLYRVGKGKAERLLKLNKGSADFVYFPDQKTVLV
                     PIMLSNALVAYKLDGAD"
     gene            complement(14528..>15119)
                     /locus_tag="VFY74_04420"
     CDS             complement(14528..>15119)
                     /locus_tag="VFY74_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5930982.1"
                     /translation="LRGSPHVRVPVVSNASFSRVGSSAAFWPVESEWDKTRMILEPDA
                     GDLIDIEALEWVKRHWFKGGRLIRKSNEFNLAMQAFDQSSFARDPVLALLLLWSALET
                     LFSPGRSELRFRCSAYIATFLEPPGIGRLSLQRATAKLYDARSAAAHGRPKDAEQPLI
                     ATFALLKRVLLKIIGEDRVPTIADIESRLFGAENES"
BASE COUNT         2669 a         4825 c         4725 g         2900 t
ORIGIN      
        1 ggccaaaacg caagcaagaa gcttcgacga cgtaccgatc aaggcaacct cccaaatgtt
       61 ttgcgacggg cggcgaatgg ctgacccaag gtcgcgcacg ctatcggaat gggactaacg
      121 gttctctaaa gctgaggcgt tgccggcagg gccgttgagg cgaaatgctg gcgggagtga
      181 cgggactcga acccgcggcc tccggcgtga caggccggcg ctctaaccga ctgagctaca
      241 ccccctttta ggaccgcaaa cgagcagtca gaggcgaccc tccctgcatg tacgggttcg
      301 gattcggcga gtcaagccga ggcccagcgg gcgctttgcg ggaggcacgc gcgtatggcg
      361 ggctggccgt cgcgcgacgg agcgcggttt atggggacgt ggcttgcccg atcaatgcca
      421 aacgaaagcc ccgcaggggg acggggcttt cgcacttcag attcggcgag gatgtacacg
      481 agcaaaacag gggtgagaag gctcgccgaa ctcagagggc tgaacgcttg cgtcgggcgc
      541 gggttccaag cctatgcgga tctccgaggc gaaaccgcgc cgccagcggc tcgtagttta
      601 gcctggtttt cgccccactc atccagcagg gccagcgacg tttcgagatc gacggccaac
      661 cgcagccacg ttttcgccaa ctcctcaaaa cgctccctgg caatggcgga cgatgaggat
      721 gtcgccaatt ccgcgcaatg cttggcgtgc tcgcggcact ctttgggatc accgggcatg
      781 agagcctctg ccagccgcat atcccccgcg cctcaatcga ttgcaaatgc cggcgctgag
      841 ttcaagcccc ttgacgtgtg gcgcaaaaaa gcgcgcgggc tgtccgtgca aagacatctc
      901 caggccccgc agcggcaacg cgtccggccc ctcccgatga gaagcggggc gcggtggaaa
      961 caaatggtgg gaggggcgtt tagcgctaca ccactaaaga gtgaggagta agacacatga
     1021 aatatctcgg aagtgccctt atcggcctgc ttggcacctt tgttttgacc accactgcgt
     1081 ctgcggccat cgtttgcaat gacgagggcg attgctggaa ggttaaagaa aaacacaaat
     1141 atccggccga cgtgaacatc caggtctatg aggacgactg gaggtggccg gaaggcgcca
     1201 agtacagatg gcgtgacgcc ggacccggcc ggggttactg gcgcggcggc gtttggatcg
     1261 acttttagca cacctatcga ggcgggatgg tgggcggtga caggctcgaa ctgccgacct
     1321 cctgggtgta aaccaggcgc tcttccagct gagctaaccg cccgccggca agaagcgata
     1381 gatcaagcgc acgcctcccg ccaccgcaaa acaaaggcgg agccttgagc cagggctccg
     1441 cctcaaaaat cggcagggtc aaggcctagc tgttgcaggc ctctttcaga gctttgcccg
     1501 cgcggaattt cggcacgcgg gacgcgggaa tgtgcacctc ggcgccggtc cgcggattgc
     1561 ggccggtcga ggccttgcgc tgcgcgacct ggaaattgcc aaagccgacg atccgcacgg
     1621 tgtctccgcc gcgcagcgct ctctcgatgt tcctgaacat cgcgtccacg gccttctccg
     1681 ccgcgtcctt cgacagatca gaatctttcg ccacatgggc gatcagttca gccttgttca
     1741 cgggtcatca tcctttcgct cgggccccaa tcgagagggt gtcgccaccc tcaaggacct
     1801 tcggtacgct ccgattcaca aataagcggt gtatgactgg cgcaaaaaac ccggaaaatc
     1861 aacaaaaatg gtccgactcc gcgaccggag ccggaccacg cgactgagcc aattggcaga
     1921 cggaaacggc ggatttccgc ggcttaatgc gccgtcacgc cgggctgggt ttcatcctga
     1981 ggcggaacga cctgagcgcc cgcttcggcc acttcctccc aggcgatcgg ctgcgcaggc
     2041 cgggtgaagg cgattttaag cacctcctcc atgcgcgaca ccgggcgaat atcgagcgca
     2101 ttcttcagct catccggtat ctccgtgaga tccttggcgt tctcgtcggg gataatcacc
     2161 gtcttgatgc cggcccgaag cgcggcgagg agcttctcct tcagcccgcc gatgggcaaa
     2221 atgcgcccgc gcagggtgat ctcgccggtc atggaaacct cgcgcctcac cggtatcccg
     2281 gtcatgagcg agatgatcgc ggtcaccatg gccacgccgg ccgaggggcc gtccttgggc
     2341 gtcgcgccct cgggcacgtg cacgtggatg tcccgcttct cgaacagcgg cggctcgatg
     2401 ccgaaatcga ccgcgcgcga ccgcacatag gcgttggccg cctgaatcga ttccttcatc
     2461 acgtcgcgca aattgccggt cacggtcatc ttgcccttgc ccggcatcat cacgccctcg
     2521 accgtgagca attcgccgcc gacctcggtc cacgccagtc cggtgaccac cccgacctgg
     2581 tcctcgagct cggcctcgcc gtagcggaac ttctgcacgc cgagatagtc ctcgaggttc
     2641 ttgagcgaaa tccggatcga tttcttcttg gtggtgagca gctccttcac cgacttccgc
     2701 gcgatcttgg cgatctcgcg ctccagattg cggactcctg cctcccgcgt gtagcggcgg
     2761 atgatctcct tcagagcgtc gtcatcgaga ccccactcct ccgcctcaag gccatgcgcc
     2821 ttggtcatcg ccgggagcag gtggcggcgc gcgatctgcg tcttctcgtc ctcggtgtag
     2881 cccgcgatcc ggatgatctc catgcggtcc atcaatgccg gaggaatgtt gagcgtgttc
     2941 gccgtggtga cgaacatcac gttcgagagg tcgtagtcga cctcaagata gtgatcgttg
     3001 aaggtgtggt tctgctcggg gtcgagcact tcgagcagcg ccgacgccgg atcgccgcgg
     3061 aaatcggcgc ccatcttgtc gacctcgtcg agcaggaaga gcggattgat cttcttcgcc
     3121 ttccgcatcg actgaatcac cttgcccggc atcgagccga tataggtgcg gcggtggccc
     3181 ctgatctcgg cctcgtcgcg cacgccgccg aggctcatgc gcacgaattc acgccccgtg
     3241 gcgcgcgcca tcgacttgcc gagcgaggtc ttgcccacgc ccggaggacc gaccaggcag
     3301 agaatcgggc ctttcagcat gttggtgcgg ttctgcacgg cgagatattc gacgatgcgg
     3361 tccttgacct tctcgagacc gtaatggtcc tcgtcgagga tcttttgcgc ctcgtcgaga
     3421 tcgcgcttga tcttgccctt gacgccccac gggatcgtca gcagccagtc gagatagttg
     3481 cggaccacgg tggcctcggc cgacatcggg ctcatctggc ggagcttctt cagctcgccc
     3541 agcgccttct cgcgggcctc gcgcgacagc ttggtcttct cgatcttctg ctcgatctcg
     3601 gcaaggtcgt cgcggccttc ctcgttgtcg ccgagctcct tctggatcgc cttcatctgc
     3661 tcgttcaggt aatactcgcg ttgcgtcttc tccatctgac gcttgacgcg gctcctgatc
     3721 ttgcgttcga cctgaagcac cgagatctcg ctctccatca gggtgaagac gcgctccagc
     3781 cgctcggaga cggaagtgat ctcgaggatc tgctgcttct cggcgatctt gatggcgaga
     3841 tgcgatgcaa tcgtgtcggc aagcttcgag tagtcctcga tctggctcgc ggtgccgagc
     3901 acctcgggcg agatcttctt gttgagcttc acatagctct cgaactgcga cacgaccgag
     3961 cgcgccagcg cctcgatctc gtcctcggcg ccgacggtct cggcgacgcg ctcgacctcg
     4021 gcctcgaaat agctctcgtt ctcggtgtag cggcgaatgg cggcgcggcg cgtgccctcg
     4081 accagaacct tgaccgtgcc gtcgggcagc ttcagcaatt ggagcacgga agcgagcgtg
     4141 cccatcgtgt agatgccgtc ggtcgagggc tcgtcatcgc tcgcgttctt ctgcgcgacc
     4201 agcagaatct gcttgtcgga ccgcatcacc tcctcgaggg ccgcgatcga cttctcgcgg
     4261 ccgacgaaga gcggcacgat catgtagggg aaaacgacaa tgtcgcggag cggcaggacc
     4321 ggatagagct ctgtctcgcc ttccctgacc gtggacggtt ttgattcagt catggcagtt
     4381 ttgtcgcttt cttttttggg cggagcgtga cggcctctcc gctcttttag tcttttaccc
     4441 ccctgcccgg cgcccggtgc caccctcaaa tattatggtc aacggcgaat agtatggcca
     4501 acgagcgcgc cgggcttgtg gcaagttgcc ccgccacttt aaccaatatg cggcgataag
     4561 ctacgtggcg acccccgatg ccgccgtcaa gtggctaaga agacggcaca gaaccccgat
     4621 tttcgccatt ttgactggga ttcgcctcgg ctcgcaagca ggaatgagcc gggcgggctt
     4681 aagcgctcgt gccgagttcc tcgtggcggt cggagtagat gcgcaaagga cgcgccttgc
     4741 cgtccaccac ctccgagctg atcaccacct cttcgacgcc gcgcaaggag ggcagctcga
     4801 acatggtgtc gagcaggatg ccttccatga tcgatcggag gccccgggcg ccggttttgc
     4861 ggtcgatcgc cttcttcgag atcgtgcgca aggcctcctc ggcgaagctg agctgcacgt
     4921 cctccatctc gaacaggcgc tgatactggc gcaccaaggc gttcttcggc tcggtcagaa
     4981 tgcgcatcag cgccgcctcg tcgaggtcgt cgagcgtggc gatgaccgga acgcggccga
     5041 tgaactcggg gatcaaaccg aacttcagca gatcctcggg ctcgacccct ttcaatatgt
     5101 cgccggtacg gcggtcatcg accgattcga ccttggcgcc gaaaccgatc gaggtcgacc
     5161 ggccgcgcgc ggagatgatc ttctcgagcc cggcgaaggc gccgccgcac acgaacagaa
     5221 tattggtcgt gtcgacctgg aggaactcct gctgcgggtg cttgcgcccg ccttgcgggg
     5281 gcaccgaggc gatcgtgcct tccatgatct tgagcagcgc ctgctgcacg ccttcccccg
     5341 acacgtcgcg ggtgatcgac gggttgtcgg atttgcggct gatcttgtcc acttcgtcga
     5401 tgtagacgat gccgcgctgc gcccgctcga cgttgtagtc ggcagcctgc agcagcttga
     5461 ggatgatgtt ctccacgtcc tcgccgacat agccggcctc ggtgagcgtg gtggcgtcgg
     5521 ccatggtgaa gggcacgtcg aggatgcgcg caagggtctg cgcgagcagc gtcttgccgc
     5581 agcccgtcgg tccgatcagg agaatgttcg actttgccag ctcgacgtcg gaattcttgg
     5641 tcgagtgatt gagccgctta tagtgattgt gcacggcgac cgagagcacg cgcttggcat
     5701 agtcctggcc gatcacgtag tcgtcgagga cggcgcaaat ctccatcggc gtcggcacgc
     5761 cgtcgcgcga cttcaccagc gagctcttgt tctcctcgcg gatgatgtcc atgcaaagct
     5821 cgacgcattc atcgcagatg aacacggtcg gtccggcaat gagcttgcgg acttcgtgct
     5881 gactcttgcc gcagaaggag cagtagaggg tatttttgga gtcggctccg ctcaccttgc
     5941 tcattcgatc atccagctct cagtcgttcg gtggcgccgg ttcacttcgg cttggcgcca
     6001 tgggtcttct tgccgcctcg caagtgttga gccccttatc tccatcgaaa gccgcccgaa
     6061 gggcaatctc agcatgctag ggcggcacta tcaaggattg attaatcccc gattctttcg
     6121 cggaacaaag cccctcgatg cattatccgg agcttgcgtg gcaacgccgg cgccggcgca
     6181 cttggtcgcg ttttcgtgat tggcgctcat gccccggccg aacgtttcgg caacaaacgt
     6241 gctcgcgctc acgagaccgg cgcgagcttt tccactttct cttccagcgg gtgccgcttg
     6301 gtaatgacct cgtcgatgag gccaaaactc cgggcgtcct cggccgacat gaaatagtcg
     6361 cgatcgagag tccgatcgac cgtgtcgtag tcctgcccgg tatggcgcac gtagatttcg
     6421 ttcagccgcc gcttcatctt gataatgtcc tcggcgtggc gctcgatgtc gctggcctgg
     6481 ccctgaaagc cgcccgaggg ctgatgcaac aggatgcggg cattggggag cgcgtagcgc
     6541 atccccttct cgccggcggc gagcagcagc gaccccatcg acgcggcctg gccgatgcag
     6601 agcgtcgcga cggccggcct gatgaactgc atggtgtcgt agatcgccat gcccgaggtc
     6661 acgatgcccc ccggcgagtt gatatacatg gagatctctt tcttcggatt ctcggcctca
     6721 aggaacagga gctgcgccgt gatgaccgag gcgacatggt cctcgatcgg ccccgtcacg
     6781 aagacgatgc gctccttgag caaacgcgaa tagatgtcgt aggcgcgctc gccccgattg
     6841 gtctgttcga ccaccattgg cacaagcgtg ctggagtaga tatcggccgg gtctggcatt
     6901 gctgtccttg gcgcggaagc ctctcgcgcg aatctgaatt gatcgtccta ctacatatgg
     6961 ggtggttgcc caaaacaaaa ggggtgacgg gagaggcccc ctacttccgc ttcttgccgc
     7021 gcttcttcgg gcctttgccg tggtcgtggc cgtgctcgtg gtcatggtca tgatcgtgat
     7081 catgctcatg gtggtgatgg gcgtggtccc cgtcctcgtc agggtcggcg taaagttcct
     7141 cggcgctcac cttcttttcc gtcaccctgg ccagttcaag gatgaaatcg actgtcttgt
     7201 cctcgaagat cggcgccctg aactcggcga ggagctccgg gttgttgcgg tagtgatcgt
     7261 agaccttccg ctcctggccg ggaaactgcc tcacgcgctc gagcagcgcc cggctcacct
     7321 cctcgtcggt gacgctgatc ttgttgcggg tgcccacttc ggagaggatc agcccgagcc
     7381 ggacccgccg tgcggcaagc tcgcgatact cctgctccgc cttttcctcg gtcgtgcctt
     7441 cgtccgcgaa gctgcgcttg ctgcgttcga gatcggcctt cacttgcgtc caaatcgcgt
     7501 tgaactcgtt gtcgacgagg gtcggcggaa gctcgaagct gtggccctcg ctcagagcgt
     7561 cgagcaatgc gcgcttgacc ttgaggcgcg agacccctcc gcgctcgttc tccatcctct
     7621 gcttgatcgt ctcgcgcaac ttgtcgaccg attcgacccc taggctcttg gcgaaatcgt
     7681 catcgagcgg cggcgtttcc ggcgcgccga cttccttgac cttgacggag aaacgcgcgg
     7741 ccttgccggc aagattggct tcctggtagg cgacggggaa cgtcgcatcc acctcgcgct
     7801 cctcgccggc gtgcgcgcct acgagcccct cctcgaaacc cggaatgaag ttgccgctgc
     7861 cgagcacgac gggagcgtcc gtggtcgaac cgccttcgaa cggcgtgccg tcgatcgagc
     7921 cggtgaaatc gatgatgagc cggtcgccgc tctccgcggc gccgtccttc gccttgtagc
     7981 gcatgttggc attgcggagg cgttcgagcg ccttgtcgat gtcggcggcg gtcaccgcgg
     8041 cgacgggcct gtccaaggcg agcttgccga aatccatcag ctcgatttgg ggcaagacct
     8101 cgaaggagag cgtataggcg aggtcgcttc taccggcgaa gatcttgtcg atctccaccg
     8161 cgtcctgcgg gagcgcgacg gtcggctgaa aggccgggcg ctcctcgcgc tgcgacagcg
     8221 cctcgcggct cgtctcggcg accgcttgct gcaccacttc ggccatcacc gagcggccaa
     8281 agactttgcg caaatgctcc ttgggcacgt gaccgggtct gaaccccttg agccgggcct
     8341 tgcccttgag ctcgtcgagc cgggcggaga gccgccgttc aagctcctca gcgccgatca
     8401 ccaccttcaa ctggcggcgc aggccctcgg tcgtcgtctc ggtgatgtcc atgtccaact
     8461 cgccttgtct atcgccacgg tggcccgagc cgaattccgc ttcctggcct ctcatgcttg
     8521 gtgcgggcgg agggagttga acccccacgg ccgaagccac cagaacctaa atctggcgtg
     8581 tctaccagtt ccaccacgcc cgcgtgagag gtcgcacccg tccgcgccat gatcgaccgg
     8641 ggtctataac acggccgatt tctcggctgc taccccaagc cgctccctcg gtgcaacccc
     8701 cgtgccgtcc gttccgtatc tcagttatga tgccgccgcc attcctccaa cccaagctgc
     8761 cttctccgtc aagctcatga gatgcttttg ccaacgcggt caagcgccgg cggtccgtgt
     8821 cctcgccgct tgcctctgtg tcgcggccgc ccttgccatc cttatcgcct tccgcctgtc
     8881 gtgggccgcg gaagcggaca gcgccgatca cgtgggccgc ataaacaagg tgcagaacga
     8941 ggcggaagtg atttcggcgt cgggtaccga ggcggccctg atcggcacgc aagtgcggat
     9001 gaaggacgag ctttgcaccg gggccgacgc gcggctgcaa gtcacctttc gcgacaacac
     9061 ggtgctgacc ctcggcgagc aggcgcgcgt tatcatcgat cgctatgtct acgatcccga
     9121 gcgcgccatc ggcgaaaccg tgcttaaggc ggcggcgggg gcttttcgtt ttgccgccgg
     9181 ccgcatggat agcctcaaag acaagaacat tcttgtctcc acgccggtcg cccagatcgg
     9241 cgtgcgcggg accgagttct ggggcggacc catcgatggg acatacggcg tgttcctgat
     9301 cgagggcgag gtcaccgtca cgagccaggg tggaagcgtc actctctcag ggtctggaca
     9361 agggattgac ctgccctcgg cgctcgaccc gcccggcccc ccttcgccct ggccggccga
     9421 aaaggtcgcc cgcgccgtcg agagcgtggc gctgcattag agtcgggctc cccggttttg
     9481 ggatgcaaat tgctgcctca agtcgctcgg agaggagcgc aacccagcgg gggccttccc
     9541 tttgaaattt ccgtgccttg caggcgttct tcgcgctgat tagtttgcct ccacccaaga
     9601 agacctgaag gctccccgtg accacccgca gcggcatcat cgaagcgatc ggcaatactc
     9661 cgttgatcaa gctcaagcgt gcgtcggagg agacgggctg caccatcctc ggcaaggcgg
     9721 agttcatgaa tcccggccag tcggtcaagg accgcgcggc cctcttcatc atccgtgacg
     9781 cgctgaacgc ggggaagctc cagccgggcg gcaccgtggt cgagggaacg gcgggcaata
     9841 caggaattgg gctcgccctc gtcggcaacg cgctcgggct taagaccgtg atcgtcatcc
     9901 ccgacaccca gtccgacgaa aagaaggaga tgctccgcct gaccggcgcc gagctgatcg
     9961 aggtccccgc ggtcccgtac aaggacccca acaactacgt gaaatattct ggccggctcg
    10021 ccgaggagct cagccgctcc gagccgcatg gcgcggtttg ggccaaccag ttcgacaatg
    10081 tcgccaatcg ccaggggcat atcgagacga cggccgagga gatctggaac gatcttggcg
    10141 gcaaggtcga cggcttcatc tgtgcggtcg ggaccggcgg cactcttgcc ggcgtctcga
    10201 tgggtctcaa ggcgcacaac ccggacgtcg tgataggact ggccgatccg ttgggcgccg
    10261 cgctgtacaa ctactacaag cacggcgaac tcaaggccga gggcagctcc atcaccgagg
    10321 gcatcggcca ggggcgcatc accaagaacc tcgagggcgc aaagatcgac gaggcctatc
    10381 agatcccgga tggcgaggcg cttcccatca tcttcgatct cttgagcgaa gagggcctct
    10441 gtctcggcgg atcgagcggc atcaacatcg ccggcgccat ccgtctcgcc cgcgatctcg
    10501 gtccgggtca tacgattgtc acggtcctgg ccgattacgg cacccgctac caaagccggc
    10561 tgttcaaccc caaattcctt cgtgagaagg gcttgccggt gccgggatgg ctcgaccagg
    10621 gacagcctgc ctgccctcgc gtgttcatct aggcttgaga cgggaggttc gttttgttgc
    10681 agaagaccaa gacatggttg acggagaccg aagaactcga gcacgagctc gatgcgcccg
    10741 acctcgtcat catcgacgcg tcctggcata tgccgggcga aggcgccgcg cgcgccgaat
    10801 atctcgccga gcatgttcca ggcgccctct ttttcgacat cgacgagatc gccgatacca
    10861 agagcagcct gccgcacatg cttccgcccc cggagaaatt ctcgtcccgc atgcggagca
    10921 tgggcatcgg cgacggctcg cgcatcgtgg tctacgattc gcagggcctc tacagcgcgg
    10981 cgcgggtgtg gtggacattt cgggtcatgg gcgccgagga tgtgagcgtg ctcaacggcg
    11041 gattgccgaa gtggaagcgg gagggacggc cattggaaag cggcgaaccg agaactcgca
    11101 cgccccggca cttcacggca cgccgcaact tggatctcgt ccgcgacctc tccgacctca
    11161 agactttgct caaggatcgg tcgaccgaga tcgtcgatgc gcgttcggcc gaacgatttg
    11221 ccgggacggt gcccgagccg cgccccggac tccgttcggg gcatatcccc ggcgcgcgca
    11281 atcttcccta cggcaggctt ctcaacaagg acggcacgct gaagtcgccg cgcgagatcg
    11341 agcggctgtt cgagggcgtg ggggtcgacc tcggcaagcc ggtcgtgacg agttgcgggt
    11401 ccggcatcac cgcgagcgtg ctggcgcttg cccttgcaga gatcggacac cgcaaaacgg
    11461 ccgtctatga cgggtcgtgg tcggaatggg gcgccgatca gagccttccc atcgaaaccg
    11521 gcaaggctta aagcgatacg gccctctgcc tggccgggac cagatgagca aacggacaag
    11581 gaagcaatca gaccggaccc gccttgcgca tgccgggcgc gcgccctcgc gccagcatgg
    11641 cttcgtcaat atgccggtct accgcggctc cacggtgatc tttccgacgc tcgcgtgtct
    11701 tgaggccgaa gaccaggatt tcagctatgg ccgactcggc acgccgacgg tgagcgcgct
    11761 cgaggaggcg atcgccgaac tcgagggggg ataccgcacg cggctcgcgc cgtcgggcct
    11821 ttcagccatc gcgaccgctc tcttcgcctt cgtcgcggcc ggcgacgaga tcctcgtctc
    11881 ggacagcgtc taccggccga cgcggcgctt ctgcgatcac gtgctgaagc ggctcggggt
    11941 caagaccacg ttctatgatc ccctgatcgg gggcgcgatc gagaggctca tcaccgacaa
    12001 gacgcgcgcg gtgttcgccg agtcgcccgg ctcgcagacg ttcgaagtgc aggacgtgcc
    12061 ggccatcgcg agggcggcgc gcaaggccgg cgcggtgatg atcctggaca acacctgggc
    12121 gacgccgctt tatttcaagc cgttcacgca cggcgtcgac gtgtcgatcc aggccgccac
    12181 caagttcatc gtgggccatg ccgacgccat gctcggcacc atcacggcaa ccgaacaggc
    12241 cttccccgcc gttttcaaga cgcatgagga gcttgggctc tgccccgggc ccgaggatgt
    12301 ctatctcggc ttgcgcggct tgcgcagcct cggcgtgagg ctcgagcgcc atcacaagtc
    12361 cgcgctcgaa cttgcgcgct ggctcggcga aaggcccgag gtcgagcgcg tgatctatcc
    12421 ggcgctgccc tcggatcggg gccacgcgct gtggcgccgc gactttaccg gcgcgccggg
    12481 gctcttctcg atcgtgctca agccgatccc tcgcgcaaag ctcggcgcca tgctcgatgg
    12541 gttgagcctg ttcgggatgg gctattcctg gggcgggttc gagagcctga tccttccctt
    12601 cgatccgcgc gcataccgca ccgcgaccaa atgggaggat aagggtcagg cgctccgcct
    12661 gcatgtcggg ctcgaagacg tcgaggatct gaaggccgat ctcgatgcgg gcttcgcgcg
    12721 cctccgcggt taagcttgag agcttgacga cacggaagga ccatcgccga tggatttcaa
    12781 actccagggg aagcgggcgc tcgtcaccgg ctcgacgcaa ggcatcgggt tcgccatagc
    12841 ccaaggtttg gcgcgcgaag gcgcgaccgt cgtggtcaat gggcgagacg agaccaagac
    12901 acgcgaagcc gccaagacga ttggcgccgc gaccaaggcg ccggtgacgg gcatcgccgc
    12961 cgatgtcggc aaggctgccg aggtcgacaa attgctgaag aagctcgggc cggtcgacat
    13021 actcataaac aatgccggca tcttcgagcc gactcccttc ttcgacatcg ccgacagcga
    13081 atggctccgc ttcttcgagg tcaacgtgat gagcggcgtg cgcctgtcgc gcgcgctgct
    13141 gccgcacatg ctcaagcaga attggggccg aatcatcttc atctcgtcgg agtcggggct
    13201 gaacatcccc gcggagatga tccattacgg catgaccaag acggcgcagc ttgccgtctc
    13261 gcgtggcctc gccgagatcg ttcccggcac gggagtcacg gtgaacgccg tgcttcccgg
    13321 gccgacgctg accgaaggtg tcgcgggctt tctcgaaagc ctcgccaagg agcgcgggct
    13381 cagcgtgaag gagatggagg tcgagctgat caagtcgatg cgtccctcct cgctgatcag
    13441 gcgcatggca acgcctgagg aggttgccaa tcccgtcgtc tatctttgca gcgaggcggc
    13501 ttcggccacg acgggcgcgg cgatccgcgt cgatggcggc gtggtgcgca ccatcggcta
    13561 gggcgttcgc cggcctagtc tgcgccgtcc agcttgtaag ccacgagcgc gttgctcagc
    13621 atgatcggca cgagcaccgt cttctggtcg gggaagtaga cgaagtcggc gctgcccttg
    13681 ttgagcttga gaaggcgctc ggccttgccc ttgccgaccc tgtagaggcc gcctgccgcc
    13741 caatcggtga cgaggtagat gccgggcgcc agcgactgaa gcccgtcgag attgccgatc
    13801 gcgtcgtcgc ggagcttgct caccgactgg tctttgagat cgacggcgat aagtcccccg
    13861 agctccggcg acccctggtc cggcagcttg ccgaacgacg caacgatcag cttgtcgccc
    13921 agcacgagga ggccgttcgg tccgttcagg gcgtcgttcg ccagccacgg ttcgaaggtg
    13981 ccgtccttga ggcgccagat cgcgttcatc ggcgtgtcgg agacatagac ggtgccgtcc
    14041 tgagcgacgg cgacgtcgtt caggaagatc gcgcccttgg ccgcgtggcg cttgacgatc
    14101 tctcccgacg caacgttgat ctcgagcagc tcgtcgacgt cggcggcata gagcgtgcgg
    14161 tcatgcagcg cgagcccggt cggcgcgttc aagcccttga cccactcgcg cgtgacgacg
    14221 gtcccgtcgg ggcgcaattt ggcgatgaag ccgttgccgt ccttctgcat aacggcgccg
    14281 gcgatgttgc taacatagag agcgcccgtc gccggatcct ggaccaccga ttccggctgc
    14341 gccaggccct tcgtttccca cagaagcttg ggcgccgcgg cggctggcgt gctaaaggcg
    14401 gcggcggacg tgcacaaggt caaagacgcg agcaggagca cgacgacagg gcgcatgagt
    14461 ttccttacct gctgtggttg aatttcggct cttaagccta ccccactttc gatttgaggt
    14521 gtgcacctca tgactcattt tcagcaccga acaatctaga ttcaatgtcc gcgattgtgg
    14581 gcacccgatc ttcacctatg atcttgagta gcacacgctt gagaagtgca aatgttgcga
    14641 taagtggctg ttctgcatct ttaggccgcc catgtgcagc ggcggacctt gcatcgtaaa
    14701 gtttggctgt ggcgcgctgg agagatagtc gaccaatccc cggcggctca aggaacgtag
    14761 ctatgtaagc agagcatcgg aagcgcaact cggatcgtcc gggagagaag agcgtctcaa
    14821 gtgcgctcca tagaagtaag agcgcgagca caggatccct ggcgaaggag ctttggtcga
    14881 acgcttgcat agccaaatta aactcgtttg attttctgat caagcggcca cccttgaacc
    14941 agtggcgctt tacccattcg agcgcctcaa tgtcgatcaa atccccagcg tcgggttcga
    15001 ggatcatgcg ggtcttatcc cactccgatt caaccggcca aaacgcagcc gaactaccga
    15061 cacgcgaaaa tgatgcgttc gacacgaccg ggactcgcac atgaggcgat ccacggagt
//
LOCUS       DASUQP010000101         6926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_588943,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000101 DASUQP010000000
VERSION     DASUQP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588943"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFY74_04425"
     CDS             complement(<1..414)
                     /locus_tag="VFY74_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX5930983.1"
                     /translation="MVTGGLYGIYSHNDGSGALTIKAYGDVTGTDISGINARNYGTDL
                     GVTTGAGTTVAGDQYGIRARNYGTGALTVTANGDVTGTNRIGIFARNYGTDLGVTTGA
                     GTTVTGDDIGIYARNDGSGALTIKAYGDVTGTDISG"
     gene            974..2149
                     /locus_tag="VFY74_04430"
     CDS             974..2149
                     /locus_tag="VFY74_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HEX5930984.1"
                     /translation="MAQRNDGVPPTAMEEKPESGAALEGASALPEGPTKPEEKVAYSV
                     SPGFSAFLGSRRISLAMSSYQSGKFYLLGQNVDGGSWCMSASSARPWGICVPGKDTIL
                     LATLFQIVKFRNVLEPDQQINNLHDGCYVPREIFVTGEVDAHDIGQLADGRPVFVNTL
                     YNCLATTSGRHSFTPLWKPPFVSKIIKEDRCHLNGLAMEGGVPRYVTAVSKSDTIDGW
                     RDRRFDGGIVIDVQSGEIVAGGLSMPHTPRLHRGKLWILNSGTGEIGWLEPAGSALEA
                     KFQVLAFCPGFVRGLAFHGKYAFVGLSKPRYQRFEGLALDRKLAETDSEPWCGVQVID
                     LDSGACVHWFRIDGPVGELYDLGVVPGVVRPMALGFATNEILGLITHEPPPDRPPWS"
     gene            complement(2181..2732)
                     /gene="moaB"
                     /locus_tag="VFY74_04435"
     CDS             complement(2181..2732)
                     /gene="moaB"
                     /locus_tag="VFY74_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX5930985.1"
                     /translation="MSRIDETRPFIPVRIAVLTVSDTRTKKTDKSGPLLVRLIEEAGH
                     ELAEHAIVEDDPKAIRKQVKRWIKDPRVDVVITTGGTGFTGRDVTPDAIRPLFEKEID
                     GFSALFHMLSYQIVGTSTVQSRACGGVAKGTYIFCVPGSPGACRDAWDGILKAQLDNR
                     HRPCSFVEIMPRLMEKKKREPAR"
     gene            complement(2802..3707)
                     /locus_tag="VFY74_04440"
     CDS             complement(2802..3707)
                     /locus_tag="VFY74_04440"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5930986.1"
                     /translation="MNIQKRILLDTGFRRYDESGILDCPVKPGNDKYGGEGEDRDGRG
                     MTATERKAVQALLDWYAAMGADEAIGEAPVDCFAPVARHALRPQAQGQSALPLRVTVI
                     PPKAGPGPGAASSATTLAELEALVARFEGCALKRTAKSLCFARGRDGARVMLIGEAPG
                     RDEDLQGKPFVGPAGKLLDRMLDATGLSEDDVYITNTVYWRPPGNRTPTPEEVEACAP
                     FLARQIELNAPDVLVLLGNAAAKTILGVTEGIMRLRGKWLTYSSGTRDIAALATLHPA
                     YLLRNPHDKRLAWRDFLALKERLDT"
     gene            3814..5448
                     /locus_tag="VFY74_04445"
     CDS             3814..5448
                     /locus_tag="VFY74_04445"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX5930987.1"
                     /translation="MEFDVVIVGAGPAGLAAAIRLKQLANEKGSDVSVVVLEKGSEVG
                     AHILSGAVIDPVALDRLFPDWRNMGSPLQTKVSEDRFYYLWKTGGIRVPNLFMPPLMS
                     NHGCYTGSLGSLTRWLAEQAEGLGVEIYPGFAVTDVIFGANRAVEGVITGDMGVARDG
                     SHKDSYAPGIALRGKYTFFAEGARGSLSRMLIKKLGLDAESGPQKFGIGLKELWEVPE
                     GKHKPGLVQHTMGWPLDDKTGGGSFLYHFGERLVSVGFVVHLDYANPYSNPFKDFQRA
                     KTHPLIRSHLEGGKRIAYGARALTEGGFQSIPRLAFPGGALIGCAAGFMNVPRIKGSH
                     NAMLTGMMAAEAAFAALGEGRANDMLEAYPEAVKASAMAKELWRVRNVKPLWSRFGTR
                     LGVMLGGIDMWLNTLFPFLPHTLKHSKPDDATLKKASSAAPIAYEKPDGVITFDILSS
                     VFLSGTNHEENQPVHLKLADPSIPIAHNLPEYAEPAQRYCPALVYEVVTEGDRQRFQI
                     NAQNCVHCKTCDVKDPAQNITWVPPEGGGGPNYVNM"
     gene            5577..>6926
                     /locus_tag="VFY74_04450"
     CDS             5577..>6926
                     /locus_tag="VFY74_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5930988.1"
                     /translation="MGIIQSSALRRWAGRSILIAAALVAAPAAYSEIGSDGARDSGTL
                     LGNYLSGRMARGDHDLSTAAEYYARALAEDPKNEMILEQAFLLDAASARWDSAVKLAK
                     DLVKNDSTNRLGRFLLGCEAFKGGRYQEADEHFAEARQGPIADLTSTLARAWVHEAAG
                     KTKEALATLDSLSDADWAQFYQRYHRALIADHAGKRDVARQAYAQAFKKNPSTLRVAD
                     AYARHAVNANNRKLARQTLKAHVAKSASHPLSDALLAEIETGKTPPLLVVTPTQGLAE
                     VFYGIGDALAGEGGLEMGIIFLQFALYLEPDFPLAHAALAEAYESAKSYQLEIDALNR
                     INANSPLWLNVQIQRAFAFNSLERIDEAKALLEQLIAKHPKDIRALDALGNILRSRER
                     YAEAQDYYSRALTLTPAPVKDNWTLFYSRGVCNERLKNWPAAEADFKKALALAPDESL
                     "
BASE COUNT         1238 a         2257 c         2148 g         1283 t
ORIGIN      
        1 gccgctgatg tcggtgccgg tgacgtcacc ataggctttg atggtcagag cgccgctgcc
       61 gtcgttgcgg gcataaatgc cgatgtcgtc gccggtgacg gtcgtgccag cgccggtggt
      121 cacgccgaga tcggtgccgt agttccgcgc gaagatgcca atccggttgg tcccagtgac
      181 gtcgccattg gcggtgacag tgagagcgcc ggtgccgtag ttgcgggcgc gaatgccgta
      241 ttggtccccc gcaacagtag taccggcgcc ggtggtcacg ccgaggtcgg tgccatagtt
      301 ccgcgcatta atgccgctga tgtcggtgcc ggtgacgtca ccataggctt tgatggtcag
      361 agcgccgctg ccgtcgttgt gggaataaat gccgtaaagg ccgccggtga ccatggtgcc
      421 ggcgccggtg gttacgccga gatcggtgcc gttgaagttt cgcgcataga tgccgttggt
      481 agaggtgccg gtgacatcgc catcggcggt gacggtcaga gcgccgcacc cgtagttgcg
      541 ggcaaggatg ccgtgattgt tgccggtgac attggtacgc gaccgtgctc accgtagcat
      601 cgtcgagcgt tatggtctgc tggttggcat tcgcgccgga acagatgaag ttcggaggtg
      661 tgccggcgac gcaggcgcca taggcgcggc cgccatagcc cgttagcgcg cccaccagca
      721 tcgtggtcga cagcaagaat tgccgacgta gggccttggc gcccattccg tcccccgccc
      781 cgaatcttgc tatttatacg cgaggaggac cagattgcgc gcctcatcct tgcaacgacg
      841 ctttgcagaa tcgctttttg gcggcacctg ttgcaggaaa ggcacagggt ccttagcgcg
      901 aagcgaaacc gccgactttg cttcattacg tcccacccga ttgctacagt cctctcccgc
      961 gatgagggaa gccatggctc agcgcaacga cggcgtcccg cccaccgcca tggaggaaaa
     1021 gccggaatcg ggcgcggctt tagagggcgc gagcgcgctt ccagagggcc caacaaaacc
     1081 tgaggagaag gtcgcctact ccgtctcccc cggcttctcg gcctttctcg gatcacggcg
     1141 aatctccctt gccatgtcgt cctatcagtc cggcaagttc tatctgctcg gccagaacgt
     1201 ggatggcggc tcatggtgca tgagcgcttc ttccgcaagg ccatggggca tctgcgttcc
     1261 cggcaaggac accatccttc tcgccacgct gttccagatc gtcaaattca ggaacgtgct
     1321 cgagcccgac cagcagatca acaatctcca cgacggctgc tatgtgccgc gcgaaatctt
     1381 cgtcaccggc gaggtcgacg cccatgacat cggccagctt gccgacggcc gcccggtctt
     1441 cgtcaacacg ctctataact gcctcgctac cacttcgggg cgccacagct tcacgccgct
     1501 gtggaagccg cccttcgtgt cgaagatcat caaggaggat cgctgccacc tgaacgggct
     1561 cgccatggag gggggggtgc cccgctatgt caccgcggtg agcaaatccg acaccattga
     1621 cggctggcgc gaccgtagat tcgacggcgg catcgtcatc gacgtgcaat cgggcgaaat
     1681 cgtcgctggc gggctctcga tgccgcatac gccgcggctt catcgcggca aactctggat
     1741 cctcaattca ggcacgggcg agatcggctg gctcgagccg gccggctcag cgctcgaggc
     1801 caagttccag gtgctcgcct tctgtccggg cttcgtgcgc gggctcgcct tccacggcaa
     1861 atatgccttc gtcgggctgt cgaagccgcg ctatcagcgc ttcgagggct tggcgctcga
     1921 ccggaagctc gccgagaccg actcggagcc ctggtgcggg gtgcaggtga tcgacctcga
     1981 ctccggcgcc tgcgtccact ggttccgcat cgacggcccg gtcggcgagc tttacgatct
     2041 cggcgtggtg ccgggcgtag tgcggccgat ggcgctcggt ttcgccacca atgaaatttt
     2101 gggcctgatc acccacgaac ctcctcctga tcgaccaccc tggtcgtgat cgcggatcgt
     2161 gatctcagaa cgcggagcgc ctagcgtgcc ggctcccttt tctttttctc catgaggcgc
     2221 ggcatgatct cgacgaacga gcaaggcctg tgccgattgt cgagctgagc tttgaggatg
     2281 ccgtcccaag cgtccctgca agcgccgggc gagccgggca cgcagaagat atacgtgcct
     2341 tttgcgaccc ctccgcacgc gcgcgactga accgtcgaag tgcccactat ctggtagctc
     2401 agcatgtgaa acagcgcgga gaagccgtcg atctccttct cgaacagcgg ccttatcgcg
     2461 tccggcgtca cgtcgcggcc ggtgaaaccc gtgccgcccg tggtgatcac cacgtcgaca
     2521 cgcggatcct tgatccagcg cttcacctgt ttcctgattg ctttcgggtc gtcctcgacg
     2581 atcgcgtgct cggccaattc gtggccggcc tcctcgatca atctgacgag gaggggcccc
     2641 gatttgtcgg tcttcttggt gcgcgtatcc gaaaccgtga gcacggcgat gcgcaccggg
     2701 atgaagggcc gggtttcgtc gatgcggctc atatgcaact tacccatcaa cagtgtcatg
     2761 cccgcaagta ggcatggtca agccgggcga tgacaattga attaagtgtc gagcctctcc
     2821 ttcagcgcca ggaagtcgcg ccatgccagg cgcttgtcgt gcggattgcg caagagataa
     2881 gcggggtgga gggtggcaag ggcggcgatg tcgcgcgtcc cgctcgaata cgtcagccac
     2941 ttgcccctga gccgcatgat gccctccgtc acgccgagaa tggtcttggc ggcggcgttg
     3001 ccgaggagga cgagcacatc cggcgcgttg agctcgatct gcctggcgag gaagggcgcg
     3061 caagcttcga cctcttccgg ggtcggcgtg cggttgccgg ggggacgcca atagacggta
     3121 ttggtgatgt agacatcatc ctccgaaagc ccggtcgcat cgagcatgcg gtcgagcaac
     3181 ttgccggccg ggccgacgaa gggcttgcct tgcaaatcct cgtcgcggcc gggcgcttcg
     3241 ccgatcagca tgacacgtgc gccgtcccgc ccgcgggcga agcagaggct cttcgccgta
     3301 cgcttgagcg cgcagccttc gaagcgtgcg accagggctt cgagctcggc aagagtggtg
     3361 gcgctcgagg ctgcgcccgg gccgggtcct gctttgggcg ggataacggt gacgcggagg
     3421 ggcagcgcgc tttggccctg cgcttgaggg cgcaacgcgt ggcgcgcaac gggcgcgaag
     3481 cagtcgactg gcgcctcgcc gatcgcctcg tcggcgccca tcgccgcgta ccagtccaat
     3541 aaggcttgaa ccgccttccg ttccgttgca gtcattcctc gcccgtcgcg atcctcgcct
     3601 tcgcccccat atttgtcatt gcccggcttg accgggcaat ccagtatccc cgactcgtca
     3661 taccggcgaa agccggtatc cagcaaaatg cgcttctgaa tattcactgg attccggctt
     3721 gcgccggaat gacggagccg aagcggcccg agcagtgaag caaccacagg acgcggagct
     3781 tgagttttga acgacgtcac agaacgcgag agcatggagt tcgacgtggt catcgtcggc
     3841 gcaggcccgg cgggccttgc cgccgccatc aggctgaagc agctcgcgaa cgaaaagggc
     3901 agtgacgtct ccgtcgtcgt gctcgaaaag ggctccgagg tcggcgctca catcttgtcg
     3961 ggtgccgtga tcgaccccgt ggcgctcgac cgccttttcc ccgactggcg caacatgggc
     4021 tcgccgctcc agaccaaggt cagcgaagat cgcttctact atttgtggaa gaccggcggc
     4081 atccgcgtgc cgaatctctt catgccgccg ctcatgagca atcacggctg ctacaccggc
     4141 agtctcggca gcctcacccg ttggctcgcc gagcaggccg aaggtcttgg cgtcgagatc
     4201 tatcccggct tcgccgtcac cgacgtgatc ttcggcgcca atcgcgccgt cgaaggcgtc
     4261 atcaccggcg acatgggcgt cgcccgcgac ggcagccaca aggacagcta tgcgcccggc
     4321 attgcgctcc gcggcaagta cacgttcttc gccgaaggcg cccgcggctc gctgtcgagg
     4381 atgctcatca agaagctcgg ccttgatgcc gagtccggcc cacagaaatt cggcatcggt
     4441 ctcaaggagc tgtgggaggt gccggaaggg aagcacaagc ccggactcgt tcagcacacg
     4501 atgggctggc cgctcgacga caagaccggc ggcggctcgt tcctctatca ttttggcgag
     4561 aggctcgtgt cggtcggctt tgtcgtccat ctcgactacg cgaaccctta ctccaatccc
     4621 ttcaaggatt ttcagcgcgc caagacccat cccctgatcc gctcgcattt ggagggcggc
     4681 aagcgcatcg cctatggcgc ccgcgcgctc acggaaggag gcttccaatc catcccgcgc
     4741 cttgcctttc ctgggggcgc gctgatcggc tgcgcggcgg gtttcatgaa cgtgccgcgc
     4801 atcaagggct cgcacaacgc catgctcacc ggaatgatgg cggccgaagc ggccttcgct
     4861 gccctgggag aaggccgcgc gaatgacatg ctcgaagcct atcccgaggc cgtaaaggcg
     4921 agcgccatgg ccaaggagct ctggcgcgtg cggaacgtga agccgctatg gtcgcggttc
     4981 ggcacgcgtc ttggcgtcat gctcggcggc atcgacatgt ggctcaacac tctgttcccg
     5041 ttcttgcccc acacgctcaa acattcgaag cccgacgacg ccaccttgaa gaaggcctca
     5101 tcggcggccc cgatcgctta cgagaaaccc gacggggtca tcaccttcga catcctgtcg
     5161 tcggtgttct tgtcgggcac gaaccacgaa gaaaaccagc cggtgcacct gaagcttgcc
     5221 gatccctcga ttccgatcgc gcacaacctt cccgaatacg ccgagcccgc ccagcgctat
     5281 tgcccggcgc tcgtctatga ggtggtgacc gagggggacc gccagcggtt ccagatcaat
     5341 gcgcagaatt gcgtccattg caaaacttgc gacgtgaagg acccggccca gaacatcacc
     5401 tgggttccgc ccgaaggggg cggcggaccg aattacgtca atatgtaggc gacgttacgc
     5461 ctatccacgc ggccacaaac cagccgcctt tacccttcaa taaagctttt ggtttgtggg
     5521 aacggggaaa agcgcctaac cttgtcggtt gggagagagg aaccgcggga catcaaatgg
     5581 ggatcatcca gagttcggcc ctgcgccgat gggcagggcg gtccatcctt atagccgccg
     5641 cgctcgttgc cgcacccgct gcctattccg agatcgggtc tgacggcgcc cgcgacagcg
     5701 gcacgcttct cggcaattat ctttcgggcc ggatggcgcg cggcgaccat gacctctcca
     5761 ccgccgccga atactacgcc cgggcgctgg ccgaggatcc caagaacgag atgattctcg
     5821 agcaggcgtt cctgctcgac gcggcctccg cccgttggga cagcgccgtc aagcttgcca
     5881 aggacttggt caaaaatgat tccaccaacc ggctcggccg cttcctgctc ggttgcgaag
     5941 ccttcaaggg aggccgctac caggaggccg acgaacattt cgccgaggcg cgccagggac
     6001 cgatcgccga tctcacctcg acgctcgctc gcgcctgggt gcatgaggcc gcaggcaaga
     6061 ccaaggaggc gctggcgacg ctcgacagtc tgagcgatgc cgattgggcc cagttctatc
     6121 agcgctacca ccgcgcgctc atcgccgacc acgccggcaa gcgcgatgtc gcccgccagg
     6181 cttacgccca ggccttcaag aagaacccct cgaccctcag ggtcgccgac gcttacgcgc
     6241 gtcacgcggt gaacgccaac aaccgcaagc tcgcgcgcca gaccctgaag gcccatgtcg
     6301 ccaagtctgc gagccacccc ctttccgacg cgctgctcgc cgagattgag accggcaaga
     6361 ccccgccgct tctcgtggtc acgccgacac aagggctcgc cgaagtcttc tacggcatcg
     6421 gcgatgcgct cgccggcgaa ggcggcctcg aaatggggat catctttctg caatttgcgc
     6481 tctatctcga gcctgacttt ccgcttgcgc acgccgcgct tgccgaggct tacgagagcg
     6541 cgaagagcta ccagcttgag atcgatgcgc tgaacaggat caatgcgaat tcgccgcttt
     6601 ggctgaacgt gcagatccag cgggccttcg ccttcaactc tcttgagcgg atcgatgaag
     6661 ccaaagcgct gctcgaacag cttattgcca agcacccgaa agacatccgg gcgctcgatg
     6721 cgctcggcaa catcctgcgc tcgcgcgagc gctatgcgga agcccaagac tactattctc
     6781 gggcgctgac gctgacgcct gcgccggtca aggacaactg gaccttgttc tattcgcgcg
     6841 gcgtctgcaa cgagcgtctc aagaattggc ccgcggccga agccgatttc aagaaagcgc
     6901 tcgcgctcgc gcccgacgaa tccctc
//
LOCUS       DASUQP010000102         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1542910, whole genome shotgun sequence.
ACCESSION   DASUQP010000102 DASUQP010000000
VERSION     DASUQP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542910"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..793)
                     /locus_tag="VFY74_04455"
     CDS             complement(104..793)
                     /locus_tag="VFY74_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HEX5930989.1"
                     /translation="MPEIDLASTPLRELNAALHRLHPDTNERHWVIDHPAGRHALAAG
                     LNVPITVEINGPVGYYCAGMNKHATVLIHGSAGVGVAENMMSGFVHVEGDASQSAGAT
                     GRGGLLRIDGNASSRCGISMKGIDIVVKGSVGHMSAFMAQAGNLIVFGDAGDSLGDSI
                     YEARLYVRGRVKSLGADCIEKPMGDEHTRALHAVLERAGFDGQIDPGEFRRYGSARKL
                     YNFHVDHAGAY"
     gene            complement(796..1686)
                     /locus_tag="VFY74_04460"
     CDS             complement(796..1686)
                     /locus_tag="VFY74_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HEX5930990.1"
                     /translation="MCGIVGLFIKDPALEPELGKLTAQMLATMSDRGPDSAGFAVYGA
                     GKAGETKLTLRAPSAEAAKETGARIAKNFPGAATTMQDTHIVVALSDESVPALLDWLT
                     REAPGIETVGRGRRIEVYKEVGLPEQVAERFGLAGMQGTHAIGHTRMATESAVTTDGA
                     HPYSTGKDQCLVHNGSLSNHNNLRRVLKREGFTFDTENDTEVAAGYLSSRMHQGLSLG
                     DALQVSLDDLDGFYTFVVGTENGFGVLRDGIACKPAVMAETDRYVAFGSEYRALAGLP
                     GIENARVWEPEPATVYFWER"
     gene            complement(1779..2495)
                     /locus_tag="VFY74_04465"
     CDS             complement(1779..2495)
                     /locus_tag="VFY74_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5930991.1"
                     /translation="MATTRLSSKGLKARNSARRRGAAAPQVPGDGGSLKPETYATGSS
                     APTTGELQLEISIGRRVRLLRQRLQLTATELAAQSGLSPGMLSKIENGGTSPSLSTLK
                     ALARALNVPLTSFFADSEERRDCSYVRAGQGVLIERRGTKSGHRYELLGHSISGDIVV
                     EPYLITLSEDAEPYALFQHDGVEFIYMLTGKVIYRHADKLYPMSSGDALFFDAGAPHG
                     PEELLERPMTYLSIIIYPRA"
BASE COUNT          463 a          852 c          901 g          473 t
ORIGIN      
        1 cggatatcgt agatgccggt cgcggcggcg cggcggatct ccgaaagcga agacgcatcg
       61 aacgtggctg aggggcgcgg ggcggttcgc ggggtccagt ccatcaatag gctccggcgt
      121 ggtcgacgtg gaaattgtag agcttgcgcg ccgagccgta gcgtcggaac tcgcccgggt
      181 caatttgacc gtcgaacccg gcgcgctcga gcaccgcatg cagggcgcgc gtgtgctcgt
      241 cacccatcgg cttctcgatg cagtcggcgc cgaggctctt caccctgccg cgcacgtaga
      301 ggcgcgcctc gtagatcgaa tcgccgagcg agtcgcccgc gtccccgaac acgatcaagt
      361 tgccggcctg ggccatgaag gcgctcatat ggccgaccga acccttcacc acgatgtcga
      421 tccccttcat ggagatgccg cagcgcgaag aggcgttgcc gtcgatgcgc aacaacccgc
      481 cgcgcccggt ggcgcccgcc gactggctgg catcgccttc cacatggacg aagcccgaca
      541 tcatgttttc ggccactccg acgccggcgc tgccatggat cagaacggtc gcgtgcttgt
      601 tcatcccggc gcagtaatag ccgaccggtc cgttgatctc gacggtgatc gggacattca
      661 gtccggcggc gagggcgtga cggcccgcgg ggtggtcaat gacccagtgc cgctcgttgg
      721 tatcggggtg gaggcggtgg agcgccgcgt tcagctccct gagcggcgtt gacgcgagat
      781 cgatctcggg cacgatcaac gctcccagaa atagacggtg gccggctccg gctcccaaac
      841 gcgcgcattc tcgatgccgg gcagaccggc aagcgcccga tattccgagc cgaaggcgac
      901 atagcggtcg gtttcggcca tcacggccgg cttgcaggcg atgccgtcgc ggagcacgcc
      961 aaagccgttc tcggtgccaa ccacaaacgt gtagaagccg tcgagatcgt cgaggctcac
     1021 ttgcagcgca tcgccgagcg agagcccctg gtgcatcctc gagctgagat aaccggcggc
     1081 gacttcggtg tcgttctcgg tatcgaaggt aaagccttcg cgcttgagca cgcggcggag
     1141 attgttgtgg ttcgagagcg agccgttgtg cacgaggcac tggtccttgc cggtcgagta
     1201 agggtgagcg ccgtcggtgg tcacagccga ttccgtcgcc atgcgggtgt gcccgatggc
     1261 gtgggtccct tgcatgccgg caaggccgaa acgctcggcc acctgctcgg ggaggccgac
     1321 ctccttgtag acctcgatcc gccgcccgcg tccgacggtc tcgatgccgg gtgcctcacg
     1381 tgttagccag tcgagcagcg cgggaacgga ctcgtcggac agagcgacga cgatatgggt
     1441 gtcctgcatc gtcgtcgccg cgcctgggaa gttcttggcg atgcgcgcgc cagtttcctt
     1501 cgcagcttct gcggaagggg cgcgcagcgt gagcttggtt tcgcctgcct ttccggcgcc
     1561 atacaccgcg aagcccgcgc tgtcgggccc gcgatcagac attgtggcga gcatctgcgc
     1621 ggtaagcttg ccgagctccg gttcgagagc gggatccttg atgaacagtc cgacgatgcc
     1681 gcacattgca agaaatctcc gggttgcgcc gcgtgcacca tgctacggcg caagggcgcc
     1741 cgtcaaccca catgaaataa actttcctgc gagtgaattc aagcccgcgg gtagatgatg
     1801 atcgaaagat aggtcatcgg ccgctcgagc agctcttcgg gtccgtgcgg cgcgcccgcg
     1861 tcgaagaaca acgcatcgcc ggacgacatc ggatagagct tgtcggcgtg gcgataaatc
     1921 accttgccgg tcagcatata gatgaactcg acgccgtcat gctggaacag cgcataaggc
     1981 tcggcgtcct cgctcaaggt gatgagatac ggctcgacca cgatgtcgcc cgagatcgaa
     2041 tgtccgagga gctcgtagcg gtggcctgac ttggtgccgc gacgctcgat caacacgccc
     2101 tggcccgcgc gcacgtagga gcagtcgcgc cgctcctcgg aatcggcgaa gaagctcgtg
     2161 agcggcacgt tgagcgcgcg cgccagcgcc ttcaaggtgg acagcgacgg ggaggttccc
     2221 ccgttctcga tcttcgacag catgcccggc gagagcccgg actgtgcggc aagctccgtg
     2281 gcggtgagtt gcaggcgctg gcgaagcagc ctcaccctgc ggccgatcga aatttcgagc
     2341 tgaagctcgc cggtggtggg tgcgctggag ccggtggcat aggtctcggg cttgaggctg
     2401 ccgccatcgc cggggacctg aggcgctgcc gcgccgcggc ggcgcgcgga gttgcgcgcc
     2461 ttcagcccct tggacgaaag tctcgtcgtc gccatcccgc ctcctcagcc ctttctgcaa
     2521 gcgttcattc tccggcttta agccgaagct agcacgatcc ggcaaaaagg gaccacgttc
     2581 cagcagggcg gcttgaacct ctttggctcc cggcgcgtcg gatacggtgc ggaatcggaa
     2641 acgatggcca gggatattgg cccaagggcg cctccgcagc ttagctttg
//
LOCUS       DASUQP010000103         7930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1406688, whole genome shotgun sequence.
ACCESSION   DASUQP010000103 DASUQP010000000
VERSION     DASUQP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406688"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..947
                     /locus_tag="VFY74_04470"
     CDS             3..947
                     /locus_tag="VFY74_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5930992.1"
                     /translation="MTKPVIAAVAALSLLTAGAPALAEDSKDPLKLTLHDWTGQLITT
                     RLMGETLKNAGLNVEYVQADYLAQFAGLKTGDLHVAMEIWATTGQEAMDEATGTGKVE
                     NLGETGMVAIEEWWYPEYMKEKCPGLPDWEALKKCAEQFSTAETKPKGRYLGAPVTWG
                     GFDEERVAALDLPFEVVHAGTDAALFAELESAMQRQAPIILWIYAPHWAPLKYKGEWI
                     EFPKYEAACYKDPAWGINKTATHDCGKPRGPVWKVAWSGVKDKWPTAYAAVKKFNITN
                     DEMGALVTKVDLEGQKVEDVVAEWMKSNEARWKGWIGQ"
     gene            1037..2065
                     /locus_tag="VFY74_04475"
     CDS             1037..2065
                     /locus_tag="VFY74_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5930993.1"
                     /translation="MTDAALACRNIWKLYGPDPKGLLARLGPAPSEAALKDAGYITAV
                     ADATLAVARGEIFVIMGLSGSGKSTLVRCLARLIEPSAGEVIFAGRNLLDASRQELIE
                     LRRHKMGMVFQHFALLPHLTVLDNVAFPLEIQGVARKEREARAREMTTLVGLKGRERN
                     LPHELSGGQQQRVGIARSLATDPDIWFLDEPFSALDPLIRREMQNEFLRLQSVLQKTI
                     VFITHDFDEAIRLADRIAIMQDGRIVQIGTPEDLVLAPADAYVVEFTREAPRAKILSA
                     RAIARARRNGEDIAGSIPADSKVAEFAKEVEASSAAFAVHDADGKLVGVVDRACVMSV
                     LIGGTEAS"
     gene            2062..4065
                     /locus_tag="VFY74_04480"
     CDS             2062..4065
                     /locus_tag="VFY74_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5930994.1"
                     /translation="MTAATTFEERASGKTPSAALVVWIGFTVLAVLSFALPDGTVPGW
                     LLEIPPGWRLGLAPAISSFMNWLIEDATFGLFTFREATRAISAFLEVPLTAATAIFST
                     GLLRGSGSTAVQLAPPLPWIAVVGIAAALGYRFGGTRLALLAGGAFLYLAVFGQWASA
                     MTTLASIAIAVPLGVAGGILFGILGYRVPLARKIIEPILDLMQTVPIFAYLVPILFLF
                     GFGPVAALIATMIYAMPPMVRVTMTALEAVPREIVEYGTMAGTTGRQMLFKVMLPSAR
                     PQLLVGVNQVIMLSLNMVIIASMIGAGGLGYDVLAALRRLDIGAGLEAGLAIVVLAIA
                     LDRLSQAYASRRAAGHDQDRTFVIALLAFTAAIYVLGLFVPAVAIYPKTLQLSTAPWW
                     GEAVRWINVHFFDTMDAIKTALLLNVLIPVKRFMLTQPWPLLTAVLAFAGWRLGGTRL
                     ALLVAALTLFIALNGQWQNAMETVYLCGISVIVAAAIGIPLGIWGGLSERAWRVLEAI
                     IDTLQTLPSFVYLIPVVMLFRVGDFTAMIAIVLYALAPAIRYTAHGIRDVQPSLIEAG
                     VAAGCTPRQLLGKIRLPLAAPVILLGVNQTVMLAISMLVITALVGTRDLGQEVYIALT
                     KADTGRGIVAGLCVAAIAIIADRLIQTAARRLAANRGERMHDA"
     gene            4055..4957
                     /locus_tag="VFY74_04485"
     CDS             4055..4957
                     /locus_tag="VFY74_04485"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HEX5930995.1"
                     /translation="MTLETQLSRAAARVAALPIWSRRVDPVPLAGGLSNQNFRVEDRG
                     RRYVVRVGDDLPVHGVVRANEVAASRAAHLVGLSPEVVHAEPGILVLDFIDGRTFSCE
                     DVQNPANLERLVDVVRRCHRDVQNYLRGSAAMFWVFHVIRDYGHTLREGNSRHLPMLP
                     ELLARAERFEAVTGPIDIVFGHNDMLPANFIDDGKRIWVVDWEYAGFNTPLFDLGGLV
                     SNSELSREQADQVLGLYFGRPVDDRLRLKARAMTAASVLREAMWSMVAEIHSTLDADY
                     VAYTAVNLRRFETAYADFQAMDAA"
     gene            4954..7404
                     /locus_tag="VFY74_04490"
     CDS             4954..7404
                     /locus_tag="VFY74_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5930996.1"
                     /translation="MSELPASAKIVIIGGGIVGCSVAYHLGKMGLTDTLLLERGKLTC
                     GSTWHAAGLVGQLRTSANITQLLGYSVALYDKLEQETGLATGWKRNGGLRLACNPERW
                     TEVKRQATTATSFGLDMHLLTPTEAQDLWPLMQIDDVVGAAFLPTDGQASPSDITMSL
                     AKGARMAGVRIAEGVKVTGIEVEDGRVHAVLAGEARIACDKLVICAGQWSRALGRMAG
                     VNIPLVPVQHQYLITEPIEGVTPNLPTLRDPDRLTYWKEEVGGLVMGGYEPNPKPWAM
                     DGLPENFEFQLLDTDLDHFEPLLELAAGRVPAMQTAGIREFINGPESFTPDGNFILGE
                     APQTRGVFVGAGFNAFGIASGGGAGMVLAEWVAKGEPPYDLWPVDIRRFGRNHLDTSW
                     VRTRTLEAYAKHYTMAWPHEEYRSGRPLRRSPLYDRLKTQGACFGEKLGFERPNWFAD
                     LARGESAQDIYTYERPNWFEAVAREHKACRERVAVFDQTSFAKFLLTGRDAEKALSWI
                     ASNDVAKPPGQLVYTQMLNARGGIECDLTVARLSPDEYYIVTGTGFATHDFDWIARSI
                     PEDADARLTDVTSSNAVLSVMGPRSRDLLQGLSEHDLSNAGFPFGTVKRITVAGSPVL
                     ALRITYVGELGWELHIPVEFAASVYDALMEAGQSHGIANAGYRAIESLRLEKGYRAWG
                     ADIGPDHSPLVAGLGWAVKLKSDVGFQGRAALEAQAKTRLPRLLAGFSADPSILLLGR
                     ETIYRNGKRVGWLTSGGYGHTVGRAIGYGYIRDPKKGVRRDELLSGRYELEVATARVP
                     AEIFFDPPYDPPMSRVKS"
     gene            complement(7420..>7930)
                     /locus_tag="VFY74_04495"
     CDS             complement(7420..>7930)
                     /locus_tag="VFY74_04495"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5930997.1"
                     /translation="DLRLAVAPTARGESTLLVQLLAPDGSIIATTSTKLRTRSEPSAD
                     PGPYEITDAPQGLNIAAAEPEAGEPEANVEERMEITNTASIETVPLPDRRPQPQPSRP
                     PGAANWIRPTAWVNLRESPSPTAPVVTIVAKGSKLRVIARKRSWVQVSNPATAQSGWI
                     YAGNLAAAR"
BASE COUNT         1326 a         2613 c         2543 g         1448 t
ORIGIN      
        1 acatgaccaa accagtgatt gccgcagttg cggcgctttc gcttttgacc gccggcgcgc
       61 cggcgctcgc cgaggactca aaagaccccc taaagctcac gcttcacgat tggaccgggc
      121 agctcatcac gacccgtctc atgggtgaga cgctgaagaa tgccggactc aatgtcgaat
      181 acgtccaggc cgactacctg gcccagttcg ccggtctcaa gaccggcgat ttgcacgtcg
      241 ccatggagat ctgggcgacg acaggtcagg aagccatgga cgaggccacc ggtaccggca
      301 aggtcgagaa tttgggcgag accggcatgg ttgccatcga ggagtggtgg taccccgaat
      361 atatgaagga gaagtgcccg ggactgcccg actgggaagc gctgaagaaa tgcgccgagc
      421 aattctccac cgccgagacg aaacccaagg gccgttacct cggcgcgcct gtgacctggg
      481 gcggctttga cgaagaacgc gtggccgcac tcgatcttcc cttcgaagtg gtgcacgcgg
      541 gaacggacgc ggcgctcttt gccgagctcg aatccgcgat gcagcgccaa gcgcccatca
      601 tcctatggat ctacgcgccc cattgggcgc cgctcaagta caagggcgag tggatcgaat
      661 tcccgaaata tgaagccgcc tgctacaagg atccggcgtg gggcatcaac aagacagcca
      721 cccacgactg cggcaagcca cgcggacctg tttggaaggt ggcctggtcc ggcgtaaagg
      781 acaaatggcc gaccgcctac gcggcggtca agaagttcaa tatcacgaac gacgagatgg
      841 gcgcgctcgt caccaaggtc gatctcgagg gccagaaggt cgaggatgtc gtggccgagt
      901 ggatgaagtc caacgaggcc cgctggaaag gctggatcgg tcagtaaatg cgagagactc
      961 gattagggct cgcagcctgc taaagtacgg caggccgcga gccctcatcg catcccgggc
     1021 acgggggccc cgatcgatga cagacgccgc tctcgcctgc cgcaatatct ggaaactcta
     1081 cggacccgac ccgaagggtc tgctcgcgcg tcttgggccg gcgccgagcg aagcggcgtt
     1141 gaaggacgcg ggctacatca ccgccgtcgc cgatgcgacc ctcgcggtcg cgcgcggcga
     1201 gattttcgtc atcatgggac tttcaggctc cggcaaatcg acgctggtgc gatgcctcgc
     1261 gcgcctgatc gaaccgtccg ccggcgaggt catcttcgcc ggccgcaacc tgctcgacgc
     1321 ttcgcggcaa gagctgatcg agctcaggcg ccacaagatg ggcatggtgt ttcagcattt
     1381 cgcgctcctg ccgcatctca ccgtgctcga caatgtcgcc tttccgctcg agatccaggg
     1441 cgtcgcgcgc aaggagcgcg aggcccgcgc gcgcgagatg accactctcg tcgggctcaa
     1501 gggccgtgaa cgcaatctgc cgcacgagct ctccggcggc cagcagcagc gcgtcggcat
     1561 cgcccgctcg ctcgccaccg acccggacat ctggtttctc gacgagccgt tctcggcgct
     1621 cgatcctctg atccggcggg agatgcagaa cgaattcctg cgcctccaat cggtattgca
     1681 gaagacgatc gtgttcatca cccacgattt cgacgaggcg atccgcctcg ccgaccgcat
     1741 cgccatcatg caggacgggc gcatcgttca aatcggtacg ccggaggatc tcgttttggc
     1801 gccggccgac gcctatgtcg tcgagttcac ccgcgaggca ccgcgggcga aaatcctttc
     1861 ggcccgcgcc atcgcccgcg cccggcgcaa cggcgaagac atcgccggca gtatcccggc
     1921 cgacagcaag gttgccgagt ttgccaagga ggtcgaggca tcctccgccg ctttcgccgt
     1981 gcacgatgcc gacggcaagc tcgtaggtgt cgtcgatcgc gcttgcgtga tgtcggtgct
     2041 catcggcggg acggaggcat catgacggcg gcgacaacct tcgaggaacg ggcgtccggg
     2101 aaaacgcctt ccgccgctct cgtcgtctgg atcgggttca cggtgctcgc ggtcctcagc
     2161 ttcgcgcttc ccgacggcac agtcccgggc tggcttctcg agatcccgcc ggggtggcgc
     2221 ctcggattgg cgcccgcgat ctcgagtttc atgaattggc tgatcgagga tgcaaccttc
     2281 ggcctgttca ccttcaggga ggcgacgcgc gccatcagcg cgttcctcga agtgccgctc
     2341 accgccgcga ccgcgatctt ctcgacggga ttgttgcgcg gaagcggatc gacggcggtg
     2401 cagctcgcgc ccccgttgcc gtggatcgct gttgtcggga tcgctgcagc gctcggctat
     2461 cgcttcgggg gcacgcgtct cgctctgctc gccggcggcg cctttctgta tctcgcggtg
     2521 ttcggccagt gggcaagcgc catgacgacg cttgcctcga tcgcgattgc ggtgccgctc
     2581 ggggtggcgg gcggaatcct gttcggcatt ctcgggtacc gcgtgccgct tgcgcgaaag
     2641 atcatcgagc cgatcctcga tctcatgcag acggtgccga tcttcgccta tctcgtgccg
     2701 atcctgttcc tgttcggctt cgggccggtc gcggcgctga tcgcgaccat gatctatgcc
     2761 atgccgccca tggtccgcgt gaccatgacg gcgctcgaag ccgtgccgcg cgaaatcgtc
     2821 gaatacggga ccatggcggg gacgacaggg cggcagatgc tgttcaaggt catgcttccc
     2881 tccgcgcggc cgcagctcct cgtcggcgtc aatcaggtca tcatgctctc gctcaacatg
     2941 gtgatcatcg cctcgatgat cggggcaggg ggcctcggct acgacgtgct cgccgcgctg
     3001 cggcggctcg atatcggcgc ggggctcgag gcagggctcg ccatcgtcgt gctcgccatc
     3061 gcgctcgacc ggttgagcca ggcctatgct tcgcgacggg cggcgggcca cgaccaggac
     3121 cggacctttg tcatcgctct tcttgcgttc actgccgcaa tttatgtgct ggggctattc
     3181 gtccccgcag tggcgatcta tccgaagacg ctgcagcttt cgaccgcgcc gtggtggggc
     3241 gaggcggtca ggtggatcaa cgttcatttc ttcgacacca tggacgcgat caagactgcg
     3301 ctcctcctca atgttctcat cccggtcaag cgcttcatgc taacccagcc ttggccgctc
     3361 ctcacggcgg tgcttgcttt cgccggctgg cgtctcggcg ggacgcgtct tgcgcttctc
     3421 gtcgcggcgc tcaccctgtt catcgcgctc aacgggcagt ggcagaacgc gatggaaacc
     3481 gtctatctct gcggcatctc ggtcatcgtc gccgccgcca tcggcattcc gctgggcatc
     3541 tggggcggcc tttcagagcg cgcctggcgc gttctcgaag cgatcatcga tacgctccag
     3601 accttgccga gcttcgtcta tctcattcct gtcgtcatgc tgtttagggt cggcgacttc
     3661 accgccatga tcgcgatcgt gctctatgcg ctcgcgccag ccatacgcta cacggcgcac
     3721 ggcattcgtg acgtgcaacc ctcgctgatc gaggcgggtg tcgcggccgg ttgcacgccc
     3781 cgccagctcc taggcaagat ccgcctcccc ctcgcggcgc ccgtgattct tctcggcgtc
     3841 aaccagaccg tgatgctcgc gatctcgatg ctggtcatca ccgcgctcgt gggaacgcgc
     3901 gacctcggcc aggaagtcta catcgccctg accaaggccg acaccgggcg cggcatcgtg
     3961 gccgggctct gcgtcgcggc aattgccatc atcgccgatc gcctgatcca aaccgccgcg
     4021 cggagacttg cggccaatcg gggagagcgc atgcatgacg cttgagacgc agctgtcgcg
     4081 ggcggcggcg cgggtcgccg cgctgccgat ctggtcgcgg cgcgttgacc ctgtgccgct
     4141 cgccggcggc ctctccaacc agaacttcag ggtcgaggat cgcgggcgcc gctacgtggt
     4201 gcgggtcggc gacgatctgc cggttcacgg ggtggtgcgc gcgaacgagg ttgccgcaag
     4261 tcgcgcggcg catctcgtgg gcttgagccc ggaagtggtg cacgccgagc ccggcattct
     4321 cgttctcgat ttcatcgatg gccgcacctt cagctgcgag gacgtgcaaa accccgccaa
     4381 tctcgagcgt ctggtcgacg tggtccggcg atgtcaccgc gacgttcaaa actatctgcg
     4441 cgggtccgcc gcgatgttct gggtgttcca tgtcattcgc gactatgggc acaccttgcg
     4501 cgaagggaac agccgccacc ttccgatgct gcctgagctg ctcgcgcgcg cagagcgttt
     4561 cgaagcggtc accggcccga tcgatatcgt gttcggtcat aacgacatgc ttccggcgaa
     4621 cttcatcgat gacgggaagc gcatatgggt cgtggactgg gaatatgccg gattcaacac
     4681 gccactcttc gatctcggcg ggctcgtctc gaacagcgag ctttcccgcg agcaggcgga
     4741 tcaagtgctc ggcctctatt tcggcaggcc tgttgacgat cggttgcgcc tcaaggcccg
     4801 agccatgacc gccgcctctg tcttgcgcga ggccatgtgg agcatggtgg ccgagatcca
     4861 ctcgaccctc gacgccgact acgtcgccta tacggcggtg aacttgcggc gcttcgagac
     4921 ggcttatgcc gacttccaag ccatggacgc ggcatgagcg agcttccggc gtcggcgaag
     4981 atcgtcatca ttggcggcgg catcgtcggc tgttccgtgg cctatcatct cggcaagatg
     5041 ggcctcaccg acacgctcct tctggagcgc gggaagctga catgcggctc cacgtggcac
     5101 gccgcggggc tcgtcggaca gctccgcacg agcgcgaaca tcactcagct cctcggctac
     5161 tccgtcgcgc tttacgacaa gctcgagcag gagacggggc tggcaacggg atggaagcgg
     5221 aatggcggct tgcggcttgc ctgcaatccc gagcgctgga ccgaggttaa gcggcaggcg
     5281 accaccgcca cgtccttcgg gctcgacatg catctgctca cgccgacgga ggcacaagat
     5341 ttgtggccgc tgatgcagat cgacgacgtg gtcggcgcgg cttttcttcc gaccgacggc
     5401 caggcctcgc cctcggacat caccatgtcg ctcgccaagg gcgcacgcat ggcaggcgtc
     5461 agaatcgccg agggcgtcaa ggtcaccggc atcgaggtcg aggacggacg cgtgcacgcc
     5521 gttctcgccg gcgaggcgcg catcgcttgc gacaagctcg tgatctgcgc cggacaatgg
     5581 tcgcgcgcgc tcgggcgtat ggcaggcgtc aacattcctc tcgtcccggt gcagcaccaa
     5641 tatctcatca ccgagccgat cgagggggtg acgccgaacc tgccgacgct ccgcgatccg
     5701 gaccgtctca cctattggaa agaggaggtc ggcggcctcg tcatgggcgg ctacgagccg
     5761 aaccccaagc cgtgggcaat ggacggcttg ccggagaatt tcgagttcca gctcctcgac
     5821 accgatctcg accatttcga gccgctgctc gagcttgcgg ccggccgcgt gccagccatg
     5881 cagacggccg gcatcagaga gttcatcaac gggcccgaaa gcttcacgcc cgacggtaat
     5941 ttcatcctcg gcgaggcgcc acagacgcgc ggcgtgttcg tcggggccgg cttcaacgcc
     6001 ttcggcatcg catcgggcgg cggcgccggc atggtgctcg ccgaatgggt ggccaagggc
     6061 gaaccgcctt acgacctgtg gccggtcgac atccgccgct tcggccgcaa ccatctcgat
     6121 acgagctggg tacgcacgcg aacgcttgaa gcctacgcca agcactacac catggcctgg
     6181 ccgcacgagg agtaccgctc gggccgtccg ctccgccgct cgccgctcta cgaccggctc
     6241 aagacgcaag gcgcatgctt cggcgagaag ctcggcttcg agcggccgaa ctggttcgcc
     6301 gatcttgcgc gcggagaaag cgcgcaagac atctacacct acgagcggcc gaactggttc
     6361 gaggccgtgg cccgcgagca caaagcctgc cgcgagcggg tggcggtgtt cgaccagacc
     6421 tcgttcgcga agtttctcct caccggccgg gatgccgaga aggcgctctc ctggattgcc
     6481 tcaaacgacg tggcgaagcc cccgggtcaa ctcgtctata cgcagatgct gaatgcgcgc
     6541 ggcggcatcg agtgcgacct tacggtcgca aggctctcgc cggacgaata ttacatcgtg
     6601 accggcacgg gcttcgccac gcatgacttc gactggattg cgcgctcgat tccggaagat
     6661 gcggacgccc gcctgaccga tgtcacctcg tccaacgccg tgctgtcggt catgggtccg
     6721 cggtcgcgcg accttctcca agggctttcc gagcacgacc tctccaatgc cggcttcccc
     6781 ttcggcacag tgaagcggat tacggtcgcc ggctcaccgg tacttgcgct ccgtatcacc
     6841 tatgtcggtg agctcggatg ggagcttcac attccggtcg aatttgccgc gagcgtctac
     6901 gacgcgctga tggaagcggg acagagccac ggcatcgcca acgcggggta tcgtgccatc
     6961 gagtcgctgc gcctggaaaa aggctatcgc gcctggggcg ccgatatcgg acccgaccat
     7021 tcgcctctcg tcgcaggcct cggctgggcc gtgaagctca agtcggacgt gggcttccag
     7081 ggccgcgccg cgctcgaagc gcaagccaag acgcgcctgc cgcggttgct tgccggattc
     7141 agcgcggatc cctcgattct tttgcttggg cgcgagacca tctatcgcaa cggaaagcgc
     7201 gtaggttggc tcacaagcgg cggctatggc cacacggtcg ggcgtgcgat cggctacggc
     7261 tatatccgcg atcccaagaa aggcgttcgg cgcgacgaat tgctgtcggg ccgctacgag
     7321 ctcgaagtcg cgacggcgcg cgtgcccgcc gagatcttct tcgatccgcc ctacgatccg
     7381 ccgatgtcgc gcgtcaagag ctaggcctga cggagccgtt cagcgcgcgg cggcgagatt
     7441 gccggcataa atccaaccgc tctgcgcggt cgcggggttg ctgacctgca cccagctacg
     7501 cttccgcgcg atgaccctga gcttggatcc cttcgccacg atcgtgacca cgggtgcggt
     7561 cggtgaggga ctttcccgaa ggttcaccca ggccgtcggc ctgatccagt tcgctgcgcc
     7621 gggcggccga gacggctgtg gctgtggccg ccgatcgggc aacggcacgg tctcgatcga
     7681 agcggtgttg gtaatttcca tcctctcctc gacattcgcc tcaggctcgc ctgcttcagg
     7741 ctccgcggcc gcgatgttca agccttgcgg cgcatcggtg atttcgtaag gaccgggatc
     7801 ggcgcttggt tcggatcgcg tcctaagctt cgtcgaagtg gttgcgatga tgctgccgtc
     7861 gggcgcaagg agctgaacca aaagcgtcga ctcgccgcgc gcggtaggcg cgactgcgag
     7921 acgcaaatcc
//
LOCUS       DASUQP010000104         6776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1134516, whole genome shotgun sequence.
ACCESSION   DASUQP010000104 DASUQP010000000
VERSION     DASUQP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134516"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFY74_04500"
     CDS             <1..322
                     /locus_tag="VFY74_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5930998.1"
                     /translation="LEPGPTASLHPLTLARYGVKPGETLRVTTRRGSIELTARADLAI
                     QQGTIFIPFAYVEAAANILTNPQLDPIGKIPEYKYCAVKIERAHGKEERVPKPSLNVT
                     AAGL"
     gene            338..973
                     /gene="mobA"
                     /locus_tag="VFY74_04505"
     CDS             338..973
                     /gene="mobA"
                     /locus_tag="VFY74_04505"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEX5930999.1"
                     /translation="MSVSEPVVGILLAGGRSSRMGGGDKCLRMLGGRPILARIIERLK
                     PQVSDMVINANGEASRFARFGLPVVADSVSGFAGPLAGVHAGLEWVRANRPGISRAVT
                     VATDTPFFPTDLVRRFLAARPGAAALIVARSGEGTHPVIGLWPVSLAAGLEAALARGE
                     RKVGAWVVAQGAVEVSFPPVEIGGRSIDPFFNINEPQHLAEADALLKEETP"
     gene            970..1485
                     /gene="mobB"
                     /locus_tag="VFY74_04510"
     CDS             970..1485
                     /gene="mobB"
                     /locus_tag="VFY74_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX5931000.1"
                     /translation="MSPPLFGVTGWKNSGKTTLTARLIAELTKRGYAVSVIKHAHEGF
                     DIDHEGRDSFKLREAGAFEVVLSSPRRFALMRELGRRPEMPFGEIVAHAGPCDLLLVE
                     GFKREDFPKIEIRRDGAASREPLHGNFPGIVAIASDRPESEGETLPVFQLDDIAAMAD
                     FIAAHVGLEPR"
     gene            1482..2774
                     /gene="glp"
                     /locus_tag="VFY74_04515"
     CDS             1482..2774
                     /gene="glp"
                     /locus_tag="VFY74_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5931001.1"
                     /translation="MTARLADDCFVLDKDRLPHEEALAILKARVRPVVVIEEVPLGEA
                     AGRFLAESIIAPRPIPAHDNAAVDGYAFSYAAYDKEKGASLAVAGEARAGHPFAGVPP
                     AGSAVRIFTGAVMPAGFDTVAMQEDVGIERGDGSPRSGERTALIPPGLKQGANRRLAG
                     EDAKAGDVLAKLGARLRPQEVAAAAASGLERLSCFAPLKVAIVSTGDEIVPPGGAFAP
                     GKVYDANAPMLDGLVRAAGARPTNLGILPDKAEQVDAALSDASRNYDAVIVSGGASQG
                     AEDHVARSIDRLGKRHLWQIAIKPGRPMSFGQIGDSVVLGLPGNPVAVFVCFLIYVRP
                     VLMVLAGGAWPEPHRFPLPAAFSQKKKVGRREFWRARLIQGADGSPRLAKFPRDGSGL
                     ISSLREADGLIEIPEDVAEVREGDLVAFIPFTEFGLPS"
     gene            2842..3531
                     /locus_tag="VFY74_04520"
     CDS             2842..3531
                     /locus_tag="VFY74_04520"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX5931002.1"
                     /translation="MRELRYPNESREYRAARDSLLKDEQELVDKVKSVAERRRNLPRG
                     GQLKEDYVFQWANDGKVGKRVRFSELFGDKNTLLLYSWMFGPNWDNPCPSCTSLVDGF
                     DRAWYSVTRDAAFAAIAKAPADRINAWAKRRGWSQIVLVSGSDSPYQADYQCQGDSDD
                     MQWPVMHVFRKQDGRIFHFWGTETTANHLDTVWVYWNLMDFTPEGRPDISTPPQNFRS
                     EFLEKNYLDKE"
     gene            complement(3712..3927)
                     /locus_tag="VFY74_04525"
     CDS             complement(3712..3927)
                     /locus_tag="VFY74_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931003.1"
                     /translation="MKSALAIAALLALLAITIGWAIHAWNAVDVEMSVHGYIAMILGI
                     FFSLLVGFGLMALVFYSNRRGSTTRRT"
     gene            complement(4299..4694)
                     /locus_tag="VFY74_04530"
     CDS             complement(4299..4694)
                     /locus_tag="VFY74_04530"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX5931004.1"
                     /translation="MRLKPPLLLLTACLAVASAPAFAQQQDLSAEYAKCIEQSGGTDP
                     GMLDCMGAEEKRQDKRLNDAYKKLMGELNPERKKELQEAQRLWLKYVEANCNFDLDPD
                     GGTAARLAATECPVLAKAARAKELENLAP"
     gene            complement(4777..5151)
                     /locus_tag="VFY74_04535"
     CDS             complement(4777..5151)
                     /locus_tag="VFY74_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931005.1"
                     /translation="MDMFGPNPNRARVSLETTRLKLPPVELTESPTPLKLLGSQAAEA
                     DEADRGATDLLIRGLVDRLPKPNAVWSLEERARWLRTAASVFGLVYKDSDGEQREIGV
                     VCANRDPAVGQGLSPGTARESD"
     gene            complement(5223..5600)
                     /locus_tag="VFY74_04540"
     CDS             complement(5223..5600)
                     /locus_tag="VFY74_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931006.1"
                     /translation="MFGRDGSAAAMEIAGVMGEAAGKSGAEIRQHGPEHDIELVLLAS
                     AGAIQRLIAERNALRTRVEVQEHELNRLQRHVTLIHDSYRRLTTEFVTQFQLIESAVS
                     NFVREPGKPTVSLAEEQEPASDR"
     gene            complement(5943..>6776)
                     /locus_tag="VFY74_04545"
     CDS             complement(5943..>6776)
                     /locus_tag="VFY74_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX5931007.1"
                     /translation="GRGGANPTLECMGDWLLNLPLPWMALLVFLAIYLATAAIYFVVT
                     RLAVDARGRAFKAVSPGMLPPLGIIFGLLVGVAVQVWNDFDRAKAAVTTEASALRGIV
                     LLAESVPEDERQKLRQVVNRHIDEAMTHEWGSMAKRQMTLAPLPTHLIEGLKVTLAVN
                     PADESQKTAQREIINELRRALDARRQRIVVSQSSVSVVKWAAILLQGFCALIAIAMVH
                     SDNRLACVIALTIFATGVAVSVLLIAAYSRPFTGEISVGPELLKQVMATERALDRSPP
                     Q"
BASE COUNT         1237 a         2120 c         2196 g         1223 t
ORIGIN      
        1 gctggagccc gggccgaccg cgagcctcca tccgctgacg cttgcccgct acggggtcaa
       61 gcccggcgag accctgaggg tgaccacgcg cagaggctcg atcgagctca ccgcgcgcgc
      121 cgatctcgcc attcagcagg gcacgatctt catccccttc gcctatgtcg aggccgcggc
      181 caacatcctc accaatccgc aactcgatcc gatcggcaag attcccgagt acaagtactg
      241 cgccgtgaag atcgagcggg cgcacgggaa ggaagagcgc gtgccgaagc ctagtctcaa
      301 cgtgacggcg gcgggcctct agccctacag ttcggccatg agcgtgagcg agcctgtcgt
      361 cggcatcctg cttgccggcg gcagatcgag ccgcatgggc ggcggcgata aatgtttgcg
      421 gatgcttggg ggacggccga tcctcgcccg catcatcgag cgcctaaagc ctcaggtctc
      481 ggacatggtc atcaatgcca acggcgaggc gtcgcgcttt gcccgcttcg gtctgccggt
      541 cgtcgccgac agcgtctcag gctttgccgg tccgcttgcc ggcgtccatg ccggacttga
      601 atgggtcagg gcaaaccgtc ccggcatttc gcgtgccgtg accgtcgcga ccgacacgcc
      661 gttcttcccc accgacctgg tgcggcgctt tctcgccgcg cggcccggcg cggccgcgct
      721 catcgtggcg cgatcgggcg aaggcacgca tcccgtgatc gggctttggc ccgtgagctt
      781 ggcggccggg ctcgaagccg cgcttgcgcg cggcgaacgc aaggtgggag cgtgggttgt
      841 ggcgcagggt gcggtggagg tgagcttccc gccggtcgaa atcggagggc gatcgatcga
      901 tcccttcttc aacatcaacg agccgcagca cctcgccgaa gccgacgcac ttctgaaaga
      961 agaaacgcca tgagtccgcc actcttcggc gtgacggggt ggaagaattc gggcaagacc
     1021 acgctcaccg cccgcctcat tgccgagctg acgaagcgcg gctacgccgt ctccgtcatc
     1081 aagcatgccc atgaaggttt cgacatcgat cacgagggcc gcgactcctt caagctgcgc
     1141 gaggccggcg ccttcgaggt cgtgctctcg tcgccgcgcc gctttgccct gatgcgcgag
     1201 cttgggcgaa ggcccgagat gccgtttggc gaaattgtcg cccatgccgg tccgtgcgac
     1261 ctcctcctcg tcgagggctt caagcgcgag gatttcccca agatcgagat ccgcagagac
     1321 ggtgctgcct cgcgagagcc gctgcacgga aactttccag ggatcgttgc gatcgcctcc
     1381 gaccggcccg agagcgaagg cgagacgctg ccggtgttcc agcttgacga catcgccgcg
     1441 atggcagact tcatcgcggc ccatgtggga cttgagccgc gatgacggcg cgcctcgccg
     1501 acgactgctt cgtcctcgac aaggaccggc tcccgcacga ggaggcgctc gcgatcctca
     1561 aagcgcgggt gcgcccggtg gtggtgatcg aggaggtccc gttaggggag gcggcagggc
     1621 gttttctcgc cgagagcatc atcgcgccgc ggcccattcc cgctcacgac aacgccgccg
     1681 ttgacggtta cgccttttcc tacgccgcct acgacaagga gaagggggcg agcctcgcgg
     1741 tcgcgggcga ggcccgcgcc gggcatccat tcgcgggggt tcccccggcc ggcagcgcgg
     1801 tgcgcatctt taccggcgcg gtcatgccgg cgggcttcga cacggtggcg atgcaggagg
     1861 atgtgggcat cgagcggggc gacgggtcgc cacggagcgg tgagaggacg gcgctcattc
     1921 cccctggcct gaagcaaggc gccaatcgaa ggctcgcggg tgaggacgcg aaagcgggcg
     1981 acgtgctcgc aaagctcggc gcgcgattga gaccccagga ggtcgccgcg gccgccgctt
     2041 ccgggctcga gcggctttcc tgctttgcgc cgctcaaggt cgccatcgtc tcgaccggag
     2101 acgagatcgt tcctcccggc ggagccttcg cgccgggaaa agtctatgac gccaacgcac
     2161 cgatgcttga cgggcttgtc cgcgcggcgg gcgcgcgccc aaccaatctc ggcatcctcc
     2221 ccgacaaggc ggagcaggtg gacgccgctt tgagcgacgc aagccgcaac tacgacgccg
     2281 tcatcgtctc gggtggggcg agccagggcg ccgaagacca tgtcgcgcgc agtatcgatc
     2341 gtctcggcaa gcggcatctg tggcagatcg cgatcaagcc cgggcgcccg atgagcttcg
     2401 gccagatcgg cgacagcgtc gtgctcgggc ttcccggcaa tccggtcgcc gtcttcgtct
     2461 gtttcctgat ctatgtgcgc cccgttctga tggtgctcgc cggaggcgcg tggccggagc
     2521 cgcaccgctt cccgctgcct gccgccttta gccagaaaaa gaaggtgggg cggcgcgagt
     2581 tctggcgcgc gcgtctgatt cagggagctg acggaagccc gcgtctcgcg aaattcccgc
     2641 gggacggctc gggcctcatc tcctcgcttc gcgaagccga cggcctgatc gagatcccgg
     2701 aggatgtggc ggaggtgagg gaaggcgacc tcgtcgcatt cattcccttc accgagttcg
     2761 gcttgccgtc ctagcgcgcc tccctcacga acttaagcgg tttttgcacg ttagcggtct
     2821 gaatgccaac aggagccgaa catgagggaa ttgcggtatc caaacgaaag cagagagtat
     2881 cgcgctgcgc gcgattcatt gcttaaagac gaacaagagc ttgtcgacaa agtaaaatcg
     2941 gtggccgagc ggcgccgcaa tctccctcgc ggcgggcaat tgaaagagga ctatgtcttc
     3001 caatgggcca acgacgggaa agtagggaag cgcgtgagat tctccgagtt attcggcgat
     3061 aagaacaccc tgctgcttta ctcatggatg ttcggtccga actgggacaa tccgtgtcct
     3121 tcctgcacgt cgctggtcga tgggttcgat cgcgcgtggt actcggtcac tcgagacgcc
     3181 gcgtttgccg caatcgccaa agcgcccgcg gaccgaatca atgcgtgggc caagcgacgg
     3241 ggatggtcgc agattgtgct cgtatccgga tcggactctc cctatcaagc cgactaccaa
     3301 tgccagggag attccgatga catgcaatgg ccggtgatgc atgtgtttcg aaagcaggat
     3361 ggaaggattt ttcatttctg gggaaccgag acaacggcga atcatctcga cacggtgtgg
     3421 gtgtattgga atctcatgga ctttacgccg gagggtcggc cggacatttc cactcctcca
     3481 caaaatttcc ggtccgaatt cttggaaaaa aactatttgg acaaagagta gcttgaagcc
     3541 cgatcgcgcg gacgaatgct tgcgaagctc gcaatcagtt cgtggatggc cggcttcgct
     3601 tcggcgaagg caggcaagtc cggcaatgac aaaaaaacgg gccaagccct gccgtgactg
     3661 ggaagggggc cgatagcgaa ccctaggctg acaacctcga gcgtcagcgg ctcaagtccg
     3721 gcgggtcgtc gagccgcgcc ggttgctgta gaagaccaac gccatcaatc cgaagccgac
     3781 cagaagcgaa aagaagatac cgaggatcat ggcgatgtaa ccgtgaacgc tcatctccac
     3841 atcaaccgcg ttccacgcat ggatcgccca accgatggtg atcgccagga gcgcgaggag
     3901 cgcggcaatt gcgagagcgc ttttcatctc accaggggtg actcgcgtgc tgcagatgga
     3961 actcaaaaaa agggaacaag acgccggaaa attggttccg atcggcgcgc ctataagcat
     4021 agccgcatca tggccgggta tctacgccgg accggacatc acgaattttc gacgcgccgc
     4081 acccgggttc ggcttcgccg gcccgggcgc aagcttgtcc gggcatccat tccgatacgc
     4141 ctccgcggaa cgcggaaaag ccaatggaat ggatggccgg gtcctgcctg cgcgaagcca
     4201 agctttgctt cggcgaaagc aggcaagcgc ggccatgaca aaaaaacggg ccaagcgcgg
     4261 ccatgacgga tgaagatagg cggatggctt gaggcgaatt acggagcgag gttctcgagt
     4321 tccttggctc tagcggcctt ggcgagcacc ggacattcgg ttgcggccaa tcttgccgcc
     4381 gttccaccgt ccggatcgag atcgaaattg cagttcgcct cgacatattt gagccatagg
     4441 cgctgcgctt cttgcagctc tttcttccgc tcggggttga gttcgcccat gagcttcttg
     4501 taggcgtcgt tcagtctctt gtcctgacgc ttctcttcgg ctcccatgca gtcaagcatt
     4561 ccggggtccg tgccgccgga ttgctcgatg cacttcgcgt actcggcgct gagatcctgt
     4621 tgttgggcaa atgccggcgc tgacgccacg gcaagacatg ccgttagcag aagcagcgga
     4681 ggcttgagcc gcatcgctca cccgagattt gagaccaccg gccaaaacag ccccgacccg
     4741 gacgggcagg gctgcgacaa gtagccgtca gaagcttcaa tcgctctcgc gcgcggtgcc
     4801 gggcgatagg ccttgtccga cggcagggtc gcgattggcg cacacgacgc cgatctctct
     4861 ctgctcgccg tcgctgtcct tgtagacgag accgaagacg ctcgccgcgg tgcgaagcca
     4921 tctggcgcgc tcttcgagag accagaccgc gttgggcttg ggcagcctgt ccacgaggcc
     4981 tcggatcagg agatccgtgg cgccgcgatc ggcttcatcg gcctcggcgg cttgagagcc
     5041 gagaagcttg agcggggtcg ggctctcggt caattccacc ggtggcaatt tgaggcgtgt
     5101 cgtctccaga gagacgcgcg cgcgatttgg gttggggccg aacatatcca tctttaccgc
     5161 acctcacctg tgatcttgcg agtccgcttc ggtctgcctg ccgcaacgcg ttcgcttgcg
     5221 ccttacctgt cggacgcggg ctcctgttcc tcggcgagcg agaccgtcgg tttgccgggc
     5281 tcgcggacga agttgctgac ggcgctttcg atgagctgga actgagtgac gaactccgtc
     5341 gtcaatctcc ggtagctgtc gtggatcagc gtgacgtggc gctgaagacg gttaagctca
     5401 tgctcctgga cttcgacacg cgtacggagt gcattccgct cggcaatgag ccgctggatt
     5461 gcgcccgccg aggcgaggag cacgagctcg atatcgtgct cgggaccgtg ttggcggatc
     5521 tccgcgccgg acttcccggc cgcttcgccc atcacgccgg caatctccat ggccgctgcg
     5581 ctgccgtctc taccgaacat gtctgctcat tctaaagtac gcgaggcatg gaaaaatgac
     5641 ctcgcttgac gccgcttcct tatctgcctt gcagtttacc ataagccgcg cgcaacggcc
     5701 tttgccgggt ttaactatgg ttaacacctt gccaaggcgc aaccctctcc ccttgtggga
     5761 gagggtggcg agccgcaggc ggcgcgccgg gtgagtgggg ggttgatgac ggcgcttcgc
     5821 gcctaacccc tcaccgcgcc gcgagcggcg ctcgctctcc ctcaacctgc ctgcgcgaag
     5881 ccggggcttc gctccggcga aggcaggggg gagagcccaa gagggcaagg ctggtcgagg
     5941 cgtcactgtg gggggcttct atcgagcgcc cgctccgttg ccatgacctg cttgagcagc
     6001 tccggtccga ccgagatttc gcccgtgaac ggacggctgt aagcggcgat caagaggacg
     6061 gacacggcaa cgcccgtggc aaagatcgtg agcgcgatga cgcaggcgag cctgttgtcg
     6121 ctatgaacca tggcgatggc aatgagggcg caaaacccct ggagcaggat cgcggcccat
     6181 ttgaccacgc tgaccgacga ctggctgaca acgatgcgct gacgccttgc gtccagtgcc
     6241 cgacgcagct cgttgataat ctcgcgctgc gccgtcttct ggctctcatc ggccggattc
     6301 accgcaaggg tgaccttcag gccttcgatg aggtgggtcg ggagcggtgc gagggtcatt
     6361 tgccgcttcg ccatggagcc ccattcgtgc gtcatcgctt catcgatatg gcgattgacg
     6421 acctggcgca gcttttgcct ctcgtcttcc gggacgctct cggcgaggag aacgatgccg
     6481 cgaagggcgc tcgcttccgt cgtgacagcg gccttggccc ggtcaaaatc gttccagacc
     6541 tgaacggcga cgcccacgag gagaccgaag atgatgccaa gcggcggcag cattcccggc
     6601 gagacggcct tgaaggcgcg cccccgggca tcgacagcaa gccgggtcac cacgaaatag
     6661 atggcggcgg tggcaagata gatggcgaga aacacaagga gcgccatcca cggcaaaggc
     6721 agattgagaa gccagtcgcc catgcactcc agggtaggat tagcgccgcc ccgtcc
//
LOCUS       DASUQP010000105         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_592208,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000105 DASUQP010000000
VERSION     DASUQP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592208"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VFY74_04550"
     CDS             complement(<1..1356)
                     /locus_tag="VFY74_04550"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX5931008.1"
                     /translation="MPTEILMPALSPTMEEGKLAKWLVREGDAVRAGDVIAEIETDKA
                     TMEIEAVEDGRLGTILVPEGTEHVKVNRPIALLVADGEGVSAVPSSEKRPAAVFEDAA
                     APLARAEEPPPPDLGEVPPLVEEAPAGPQIAPAEPRPDAEVPPGTAMVKLTVREALRD
                     AMAEEMRADPAVFVIGEEVGEYQGAYKVTQGLLDEFGPARVVDTPITEYGFAGVGVGA
                     AFAGLKPIVEFMTWNFAMQAIDHIINSAAKTLYMSGGKMRCPIVFRGPNGAAARVAAQ
                     HSQDFSSWYAHVPGLKVIAPYTSADAKGLLKAAVRDPSPVVFLENEILYGQSFEVPDL
                     PDFVLPIGKARVARAGRHVTIVSFSRGMAYALDAAKRLAEEGIEAEVIDLRTLRPLDI
                     ETVLASVRKTNRLVTVEEGWPVGSIGSEICARVATDAFDYLDAPPLKITGADVPMPYA
                     AN"
     gene            complement(1376..>2044)
                     /locus_tag="VFY74_04555"
     CDS             complement(1376..>2044)
                     /locus_tag="VFY74_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5931009.1"
                     /translation="HMFSVEKNFYGGHGIVGAPAPLGTGLAFANKYRGNDRVCLTFFG
                     EGAANQGQVYESFNMASLWKLPVIYLIENNHYAMGTSVERASARAEELYHRGSVFGIP
                     GEQVDGMDVAAVKAAGDRAVAASRRGEGPKILELLTYRYRGHSMSDPAKYRTREEVQE
                     MREHRDPIELLRNRMLASGHASEADLKEIDREVRAIVTEAAEFAQSDPEPDPSELYTD
                     VTAN"
BASE COUNT          338 a          687 c          673 g          346 t
ORIGIN      
        1 attggccgcg tagggcatcg gcacgtcggc gccggtgatc ttgagcggcg gcgcgtcgag
       61 atagtcgaag gcgtccgtgg cgacgcgggc gcaaatctcg gagccgatgg agccgaccgg
      121 ccaaccctcc tccacggtga cgaggcgatt ggtcttgcga accgaagcaa gcaccgtctc
      181 gatgtcgagc ggccgcagcg tgcggaggtc gatcacctcg gcctcgatcc cctcctccgc
      241 gagccgcttg gccgcgtcga gcgcgtaagc catgccgcgc gagaaggaga cgatggtaac
      301 gtgccggcct gcgcgcgcca cgcgcgcctt cccgatcggc aagacgaagt ctggaaggtc
      361 ggggacctcg aagctctggc cgtacagaat ctcgttttcg aggaacacca cggggctcgg
      421 atcgcggacc gcggctttca acaggccctt ggcatcggct gaggtgtagg gcgcgatcac
      481 cttaagcccc ggcacgtggg cataccagct cgaaaaatcc tggctgtgct gggccgcgac
      541 gcgggcggcg gcgccgttag gcccgcggaa cacgatcgga cagcgcatct tgccgcccga
      601 catatagagc gtcttggcgg cagagttgat gatgtggtca atcgcctgca tggcgaagtt
      661 ccaggtcatg aactcgacga tgggcttgag cccggcgaag gccgcaccca ccccgacacc
      721 ggcgaagccg tattcggtga tcggcgtatc gacaacgcgc gcgggtccga actcgtcgag
      781 caggccttgg gtcactttgt aggcgccctg gtactcgccg acttcctcgc cgatgacgaa
      841 gacggcgggg tctgccctca tctcctcggc catggcgtcg cggagcgcct cgcgcacggt
      901 gagcttgacc atcgccgtcc ccggcggcac ttcggcgtcg gggcgcggct cggccggggc
      961 aatttgcgga ccggccggcg cctcctcaac gagcggcggg acctcgccga gatcgggagg
     1021 cggaggctcc tccgcacgcg caagcggtgc cgcggcgtcc tcgaacacgg ccgcaggccg
     1081 cttctccgaa gacggcaccg ccgagacccc ctcgccgtcg gcgacgagga gcgcgatcgg
     1141 ccggttgacc ttcacgtgct cggtgccctc gggaacgagg atcgtgccga gccgcccgtc
     1201 ctcgaccgcc tcgatttcca tcgtcgcctt gtcggtctcg atctcggcga tgacgtcgcc
     1261 cgcacgcacg gcgtcgccct cgcgcacgag ccacttcgcg agcttcccct cctccatggt
     1321 gggagacagc gccggcatga ggatctcggt tggcatcacg ctctgcgagg attgctcaat
     1381 tcgccgtcac gtcggtgtaa agctcggaag gatcaggctc gggatcgctt tgcgcaaact
     1441 cggcggcctc ggtgacgatc gcgcgcacct cgcggtcgat ctccttcaaa tcagcttcgc
     1501 tcgcatggcc cgaggcaagc atccgattgc gcaacagctc gatcgggtcg cgatgctcgc
     1561 gcatctcctg gacctcctcg cgggtccgat atttggcggg gtccgacatc gaatggccgc
     1621 gatagcgata ggtgagcaat tcgaggatct tcggcccctc cccgcgcctc gaagcggcca
     1681 cggcgcggtc gccggccgcc ttgaccgcgg cgacatccat gccatcgacc tgttcgccgg
     1741 gaatgccgaa caccgagccg cgatgataga gctcctcggc gcgcgcgctc gcgcgctcta
     1801 ccgacgttcc catggcgtag tggttattct cgatgagata gatcacggga agcttccaca
     1861 gcgacgccat gttgaagctc tcatagactt gtccctgatt ggccgccccc tcgccgaaaa
     1921 aggtgaggca gacacgatcg ttgccgcgat atttgttggc gaaggcaagg ccggtgccga
     1981 gcggcgccgg tgccccgacg atgccgtggc cgccatagaa attcttctcg accgagaaca
     2041 tgtg
//
LOCUS       DASUQP010000106        54909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1955821, whole genome shotgun sequence.
ACCESSION   DASUQP010000106 DASUQP010000000
VERSION     DASUQP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 54909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54909
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955821"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..321)
                     /locus_tag="VFY74_04560"
     CDS             complement(157..321)
                     /locus_tag="VFY74_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931010.1"
                     /translation="MMKTLISTFALALALGFAGPALAGDVSKASNAADCEKAGGTWDA
                     NTSKCEEKKM"
     gene            854..1051
                     /locus_tag="VFY74_04565"
     CDS             854..1051
                     /locus_tag="VFY74_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5931011.1"
                     /translation="MNWDRIEGNWKQFTGRVKQQWGKLTDDDLQQVEGRRDQLVGKIQ
                     ERYGIAKDEAERQIDSWLTNV"
     gene            1152..1304
                     /locus_tag="VFY74_04570"
     CDS             1152..1304
                     /locus_tag="VFY74_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX5931012.1"
                     /translation="MTTILIILLILILIGALPTWPHSRGWGYGPTGIVGILLIVLLVL
                     MLAGRV"
     gene            1354..1500
                     /locus_tag="VFY74_04575"
     CDS             1354..1500
                     /locus_tag="VFY74_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931013.1"
                     /translation="MPRWLTRTEARQGAGPRAMLWVLLASLALACAAGLALGLGWITL
                     PWTG"
     gene            1571..2575
                     /gene="glk"
                     /locus_tag="VFY74_04580"
     CDS             1571..2575
                     /gene="glk"
                     /locus_tag="VFY74_04580"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX5931014.1"
                     /translation="MDQALGLPEAILVADLGGTNARFAIAELASLTLSHVIQFPCQMH
                     RGPEAAIRAYLEQVDATPKYASIAIAAPVTGDDVVFTNSYWSFKIGALCRDLGFNDVR
                     VLNDFEAFALALPSLGSQEVLQIGGTEPQMRGTKVAIGVGTGTGMAALVWSPAGWVAV
                     PSEGGHASFACQTAEDFALAEELRMDRSHLSAERILSGPGVSALHQAVAALHGVEVEA
                     LEPNDVLTRGLAGADALAVEALRFFVRCLGRFAGDAALTFGARGGVYLGGGITPRILD
                     LLKAGPFRDAFEDKGRMRGYLAPIPIYAVLAEHATLRGAAIGARMNLASSSAFPLGLS
                     "
     gene            2597..4381
                     /locus_tag="VFY74_04585"
     CDS             2597..4381
                     /locus_tag="VFY74_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX5931015.1"
                     /translation="MSDFGLDLAVIGNCHTAALLEPTSRLVWWCYPRFDGDPVFSRLL
                     AGDDEKGFCDVVLENMVDFSSGYERNTAIVSTVLTDAQGAAVKITDFAPRFRQYGRVF
                     RPAQLIRIIEPITGLPRIAIRVRPTSDYGKPIETSALRSSHISYQGKNAAIRLTTDGP
                     LSYIDQESPFALTKPIHMVLGPDEPFAEDIATTCREFADRTHDFWMDWVRRLSIAYDW
                     QDPIIRSAITLKLSNFEETGAIVAALTTSIPEASGAGRNWDYRYCWLRDAYFVVKALN
                     RLGATETMESFISFILGIAAQDPLRPVYGIVPTEPLDEVIAPSLKGYRSDGPVRIGNA
                     AALQHQNDAFGSIVLAAMPMFFDRRLPRPGNEGLFHFLESLGSRAAEAAFTPDAGIWE
                     YRSRAHVHTYSAAMCWAAASRLAAIAAHLGLIDRATHWADTASKIQDALLEQAWNEKR
                     QAFTSAFGSEHLDASVLLLPDIGLIEPTDPRFTQTVAAVEKDLLRGKHVLRYANDDDF
                     GMPEVAFLACRYWLIDAWWSLGRREEARELFIDSLRYRNRYGLLAEDIHPATGELWGN
                     FPQTYSMAGLILTAMRLSRSWEDRYWRG"
     gene            4369..5832
                     /locus_tag="VFY74_04590"
     CDS             4369..5832
                     /locus_tag="VFY74_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX5931016.1"
                     /translation="MARLVLVSNRTGLPQGDGTQRAGGLEVALAPILTGISTLWFGWS
                     GKVVPPEKVRTETVDRNDATYVVTDLAQDDYEEYYNGFANRVLWPVLHYHIDLTEFTR
                     RDLSGYFRVNRHFANELSKILQPDDLVWVHDYHLIPIAEELRQRGHDNALGFFLHVPF
                     PAPEVMTVLPNHEQLLPLLLQYDLVGLQTETDVGNFVRYLIAERRGSDRRVIEATGGQ
                     FLTGGEFAFSSRGRQTRIGSFPVGIDGAEFERLARRAVKSTFVRDVVESLSARALLIG
                     VDRLDYSKGLVQRMDAIERFLDMYPAWRNKITYLQIAPKSRSEIPTYAELEQAVGAAV
                     GRINGRYGEVAWTPIRYLNRAFSRSNLAGLYRSARVAIVTPLRDGMNLVAKEYIAAQD
                     TEDPGVLVLSRFAGAAVECEAAVLVNPYDPESVARAIAQALDMALPERRKRHEANLAA
                     LARNRHNEWSTRFLQSLRNAPKAANRPAAAGRLDALF"
     gene            complement(5839..7992)
                     /locus_tag="VFY74_04595"
     CDS             complement(5839..7992)
                     /locus_tag="VFY74_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX5931017.1"
                     /translation="MLEPKPEPLFDIAVTSSSARPRYTMKQDDSFLVLDSHGDVGVSV
                     GGPDGLFYRDTRYLSRFELLINGVAPLLLGSTLSNDNCVLRVDLTNPDFRDRDSVVLS
                     KNLLHIARTLFISRETLHQRLTLHNYGADSVQLLLSLAFESDFADVFEVRGVVRRRRG
                     VERAEISETRATLTYAGRDGVMRRTHIAFDPTPLTLTEDCASYRLDLSPHSVASLYTR
                     VSCDSRSEETLFLRTMRRAHLERKKLMRVETKIHTSNHSFNEMLRRATADLAMLLTET
                     PEGYYPYAGIPWFSTTFGRDGLITALQCLWLDRRIPIAVLRRLAATQATAFDAASDAA
                     PGKILHEMRAGEMANLKEVPFGRYYGSVDSTPLFVLLAGQYVQSTGDVATLQELWPAI
                     ERALAWIDGPGDPDRDGFVEYDRQTDKGLINQGWKDSQDSIFHSDGRLATGPIAVAEV
                     QGYVYAAKRLAAWCAETLNLPDRARQLGSEADLLAERFEDSFWCGELGTYALALDGDK
                     VPCRIRSSNAGQVLLSGIARPDRARKVASGLMQPDSFSGWGIRTISTAEPRYNPMSYH
                     NGSVWPHDNALIALGFARYRAKGEVTRIFRALFEAASYLDLQRLPELFCGFPRLAGRG
                     PTSYPVACAPQAWSAGSFFALLQASLGIVQDPWKREIRFVKPTLPKFLDEVSLRGLRA
                     GEGAVDVLIRRYGERTALEVVRTWGHVDVKTESDD"
     gene            complement(8016..9134)
                     /locus_tag="VFY74_04600"
     CDS             complement(8016..9134)
                     /locus_tag="VFY74_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5931018.1"
                     /translation="MKIAQIAPLMESVPPRLYGGTERVVSYLTEELVGLGHDVTLFAS
                     GDSITEAAFVPCCKSALRLEAEAHDPIPHYIVMLHKLMRVAHHFDVLHFHIDYLHFPQ
                     IGGLESRAVTTLHGRQDLPANAPFYRYFRNTPLVSISHAQRAPISYANFIATIHHGLP
                     LDLHRAEFDPRGGYLAFLGRISPEKDPVQAIRIARELNMELKIAAKVDRADEHYFREE
                     VKPLLGLPGIEFVGELGEKEKGHFLRNASALLFPICWPEPFGLVMIEAMACGTPVLAF
                     DWGSVPEVIDDGVTGRVVTTFEEAVAKLPQIVKLDRRKVRQRFEKRFSARRMANDYLR
                     VYQSLVRPSRVGQPRQPVSGQTINLTGDAVHARIYAAE"
     gene            9813..10310
                     /locus_tag="VFY74_04605"
                     /pseudo
     CDS             9813..10310
                     /locus_tag="VFY74_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            complement(10702..11721)
                     /locus_tag="VFY74_04610"
     CDS             complement(10702..11721)
                     /locus_tag="VFY74_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5931019.1"
                     /translation="MSKRSPRKSASRGSFEREARATARHQRQIQREANRSDEPKTEGE
                     EEQDAMQAGTRDYPVPPLPKQHLKKPGSEADLDPEPMYDAPHYKGSEKLEGKVALITG
                     GDSGIGRSVAVLYAREGADIAIAYLEEHADAEETKRSVEKEGRRCLMLSGDVADPKFC
                     QHAVKQTLAEFGQLDILVNNAAFQQHVTSLEDLSDEQFDQTIKTNLYGYFYMTKAAVP
                     HMKEGSAILMTGSVTGILGSKDLLDYSMTKGGIHAFTRSLATHLVDRGIRVNAVAPGP
                     VWTPLNPADQSAEDVAEFGSSTPMKRPAQPEEIAPAFVFLAAPSCSSYITGEVLPIIG
                     GYSRG"
     gene            12075..13148
                     /locus_tag="VFY74_04615"
     CDS             12075..13148
                     /locus_tag="VFY74_04615"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX5931020.1"
                     /translation="MAGAVALAVPLLSGSPVLNPAALAAPAASTESFVQTVAMSDMFE
                     VQSGKLAADQAGNAKVKSFGQKMVDDHTKTTNELTQLIKDENITVDLPTALDRDHQAK
                     LDDLKGLSADAFDRAYIPTQVSAHEKAVDLFQGYADGGENADLKQWAQKTLPALKGHL
                     DEAKQLSSDIKMAANDKDGQATTKDRQAEVTVETNKDDDVRVEARQRKDRAASFEYVS
                     RQKPTDWTAQALIGRTVENDAGDNLGDINNVILNEKGDVVAVTIGVGGFLGIGEKNVG
                     VPFQALEFRTDEARADRATERNDADEKKESADKRREARFDTEHSNIRIVLDATKEQLE
                     AAPEFVWLDEQSTGSAREERAVQ"
     gene            13881..14150
                     /locus_tag="VFY74_04620"
     CDS             13881..14150
                     /locus_tag="VFY74_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931021.1"
                     /translation="MKKPPARSQKQQSTFDSNELEIIDRVYHAAWAKILTRHPGHDID
                     KAEQRQTALRKRLFILAARGPVEFDTLRDRVLVTMPETLMNPTRR"
     gene            14160..14402
                     /locus_tag="VFY74_04625"
     CDS             14160..14402
                     /locus_tag="VFY74_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931022.1"
                     /translation="MSRQTGSYVRALKLTAFLSALVGAILLQAVEPATAALKHVIIIE
                     AVDVGFPALIHEETPEHMEIAPANTISVEKEPAAAR"
     gene            complement(14416..15306)
                     /locus_tag="VFY74_04630"
     CDS             complement(14416..15306)
                     /locus_tag="VFY74_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5931023.1"
                     /translation="MVGAGAAGLSAALVLGRCRRSVSLFDAGRPRNRASRAVHCLLGQ
                     EGTPPAELLAKGRSELARYPTVTGVPDPVTEITPLDGRFLVAAASGDTVNVRKVLLAT
                     GVTDEIPQIDGIDTLYGRSVHHCPYCDGFEHRDQAIAVYGTGDKGAGLALMMKQWSRD
                     VMLFTGGASPISADLLERLGQQAIEVRTEPISRLEGTGDGALRRIHLQNGEAVARTAM
                     FFTTGCRQGSDLSTRLGCARDEKGGIVTDPVTEESSVKGVYVAGDASREVLLVAVAIA
                     EGAKAGVAINRALLKEEGLG"
     gene            15537..16475
                     /locus_tag="VFY74_04635"
     CDS             15537..16475
                     /locus_tag="VFY74_04635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009604.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HEX5931024.1"
                     /translation="MRLLILVNPGASRADAALEELAAWFQQNCDATFVTTRSRDHLTQ
                     ILLRHGHDVDRIVIGGGDGTISEALPCLLDLGKPLAVLPLGTANDFARSLGLPRDSFA
                     AARIALDGPTHKVDVGLVNGRPFINVASVGLAAQVSQVQSKELKRIWRLFSYAIGLLR
                     AVIHSRPFRVDLTIDQHWTWTGLAYQVSVANGRYHGGGLRVAEHAAIDDGKLNVYFVL
                     PGKVWQLVAAIIHLKFGLTKPEILQQKSGRHVTIRTRRPRPVNADGEIGTATPADITL
                     RPKALSVIVPPLGGGEESTLVTQTGQARPVDRDAPR"
     gene            complement(16487..16984)
                     /locus_tag="VFY74_04640"
     CDS             complement(16487..16984)
                     /locus_tag="VFY74_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5931025.1"
                     /translation="MEPDTADAALRLASAVLVGVLVGLNRDLAGKPMGMRTLALVSLG
                     AAVVSVATIHFQNLDDHPDALSRVVQGIIQGVMGGIGFIGAGVILRDHKAHTVEGLTT
                     ASTVWLTAALGIACGLAAWNVIAIAVIFALATLVGIYWLESAYLERLAGKKLRLRREK
                     RGRSG"
     gene            17211..18251
                     /locus_tag="VFY74_04645"
     CDS             17211..18251
                     /locus_tag="VFY74_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5931026.1"
                     /translation="MSPRPARPNDFDEFVKRSLFVAALVGLGILLWFLWGVLLLIFAG
                     ALLAIAIRDLAMPFARWTPLGTGGAIALVVILLLVVLSGTGLVLGTQLIAQLGELWTR
                     LPEFAGSARQFIGQAPIGRTVLQLLPDWETIFRGITAGSALTAATTTLGVVANVLIVI
                     FLALFFALTPRLYTRGLVALLPPAHRARGQEVLAETAKALRAWLRGQFVAMIAVGVVT
                     WIGLTLLGVPLALGLAFLAFLLEFIPVLGPIAASIPAILVGLSISPLLGLWVALLYIV
                     IQQVESYLLLPLIQRWAVNLPPALTLIGIVAFGVLFGWIGVLLATPLMVATIVWVREV
                     YVESILEPEKKQ"
     gene            18330..18713
                     /locus_tag="VFY74_04650"
     CDS             18330..18713
                     /locus_tag="VFY74_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX5931027.1"
                     /translation="MTKATAHPDHRLISSEDVHGTNVYGVGDEAIGEIDHLLIEKVSG
                     RVSYAVMSFGGFLGLGHSHYPIPWSALKYDTALGGYRTGITEGQLRDAPEFSDDSWED
                     RDWETRTHEHYGAPPYWGDGRREAA"
     gene            complement(18736..19182)
                     /locus_tag="VFY74_04655"
     CDS             complement(18736..19182)
                     /locus_tag="VFY74_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5931028.1"
                     /translation="MAAQRALIVENEPLIAIDLEAILRALGFDVCGLASNPREAVELA
                     TSRRPDLVLMDVYLDEGLQGIKAAKWLRDACDIPVLFVTGHSDPDTVARIREALPEVH
                     VCAKPVAPHRLAEAIAGATGWQVPSGPAPSWPEMKEARSSWGRASA"
     gene            19368..19730
                     /locus_tag="VFY74_04660"
     CDS             19368..19730
                     /locus_tag="VFY74_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931029.1"
                     /translation="MVAMHVLSRTAAALVAALLAAGLMVGAALAAASDFEGTWKTQDT
                     KGQAFEIILSADGKAKGSRADEGLNGTWKVDGDSALITWDSGWTTKIVKEGDKFKKLA
                     FEKGQSGDPTHTADAQKM"
     gene            19954..20154
                     /locus_tag="VFY74_04665"
     CDS             19954..20154
                     /locus_tag="VFY74_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931030.1"
                     /translation="MNTHVTDEDVSVGPPKPKDRRVLMTLIGTVAVIVLALIAFVALS
                     DFPGPSEGSADKATAPAEDVRR"
     gene            20214..20447
                     /locus_tag="VFY74_04670"
     CDS             20214..20447
                     /locus_tag="VFY74_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931031.1"
                     /translation="MARHIVMDPSGHSTFEFNPGSLADLAKAERRFNKLLNKGYVPAY
                     QRGEGTHRVPPQENRSFDPSAEETIFIPALRGG"
     gene            20465..20815
                     /locus_tag="VFY74_04675"
     CDS             20465..20815
                     /locus_tag="VFY74_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931032.1"
                     /translation="MVAIVVLSGLAMLVCSIRQRMAETKGLTLLVSNLSEEQRRQYEA
                     FGYFDAIGSITGKRYRIHHGNSRNVVQLGSGNLRSGRCFAPRGDLVPGDCMLAQKIAI
                     ENYEAEVLAKALPF"
     gene            21327..23096
                     /locus_tag="VFY74_04680"
     CDS             21327..23096
                     /locus_tag="VFY74_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5931033.1"
                     /translation="MTVDQGLAFAIVTIMMVLFAWGRWRYDLVAAMALLAAVTVGIVP
                     PDQAFKGFSDDIVIIVGSALVVSAAVARSGVIERVARSLGPRLVTIHRQIIALVGSVT
                     LLSAFVKNVGALAMTMPIAFQLARRSGTSPSSLLMPMSFGALLGGTMTLVGTSPNIIV
                     SRMREQLLGSPFGMFDFTPVGAGIVLAGFLFLVFGWRLLPRHRKGAAAIDAAFNLEAY
                     TTEVRVPEESPAAGVTVKALEDLGENEIEVITLLRGGTRRYEPAGNALIKAGDILILR
                     GEPSALERVIVLEKLKLTREGKRHAIDTPSDEIGVMEAVITPDSELVGSSAGQLQLFD
                     RHAINLLAVSRKGRHIHRLRAVKLRAGDVVVFGGNIAAMPETLGELRCLPLAGRDLRI
                     GRKASFLPLAVLAAAMALVAVNVLPVAIAFFGAAITLLLVRSLSLRQAYEAIEWPILI
                     MLGALIPVSDALRTTGGTDLIAAWLSSAASALPPMGALALLLVSAMAVTPFLNNAATV
                     LVMAPIAAGFAGRLGYSPDPFLMAVAIGAACDFLTPIGHQCNTLVMGPGGYRFGDYWR
                     LGLPLSLIVIAVGVPLIAFFWPF"
     gene            23206..24048
                     /locus_tag="VFY74_04685"
     CDS             23206..24048
                     /locus_tag="VFY74_04685"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HEX5931034.1"
                     /translation="MKRQTSTLITAAAFLVAFGLTAAQAGAQSDPMAPGGGPAGSIGA
                     GEGGGMAPASPGGGPNQPGNAPGASDMMDTGPGSAASKGSTAEAPPSAKEPKGAAGTQ
                     TGMPSDRKDSRPDGDKAGTTAEGQPDTKDGMSKDDKQGATPEGKDDKEGMSKDDKPGA
                     TAEGKDDKDGMSKDDKPGATAEGKDDAKSKGKSARLESKDISKVRSHFSQNKPNVKRI
                     DRDDFSVSIGIALPGSIVLYDLPPDVIVVSGPCPIQYFVWGDDIVLVDSCSREVVEII
                     AGVA"
     gene            24212..24556
                     /locus_tag="VFY74_04690"
     CDS             24212..24556
                     /locus_tag="VFY74_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX5931035.1"
                     /translation="MAIKHAIRQVLTRFGELAAHPLAFGIWLLYAVVWLILDRDSLDW
                     HGVATMVTWFMTLIIQRAEDRDTQALQAKLDELLRVIKPADDALTEIDTLEPEDIKRL
                     RKETHSSSGEKT"
     gene            complement(24553..25581)
                     /locus_tag="VFY74_04695"
     CDS             complement(24553..25581)
                     /locus_tag="VFY74_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX5931036.1"
                     /translation="MILETFERWRRGRNWGYGFAIIAFIVAFAVRYGLEDLLPPGLPF
                     LTFFPAVILTSFFAGVGPAVLVTALSVLAAWYFFLPQLQSFNIESDSVIALVLYVFIN
                     ALIILLVHTLNVAIERLREERGKARALADQSEAMFAELQHRISNNLQIVAALLNLDRE
                     RVTDRGATKALSDASRRLELIGKLHRKLYDPQGAAIDFGAFLKGLCDDIVKSWNALDV
                     DCLVKSVTVKLPADQAIPIALIATELISNSLEHGLAGRPRGTINIDLRQNGGDSLVLE
                     VADDGNGLPPGFDLAATKSLGLNIVQILTQQLAGRLEMQGGGGTISRIVFPARMVRSV
                     TIAASQSS"
     gene            25726..26106
                     /locus_tag="VFY74_04700"
     CDS             25726..26106
                     /locus_tag="VFY74_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5931037.1"
                     /translation="MVSSAPVILVVEDEYIIRAALVAELEQAGWKVLEARTGEAALAQ
                     LRGEQRIDVLVTDIQLAGYLSGWEVAEGFRAATPDIPVVYMSGKAVDKSRMVPGSVFL
                     SKPFAPQDVLAAARETMQMSQRTA"
     gene            complement(26150..27241)
                     /locus_tag="VFY74_04705"
     CDS             complement(26150..27241)
                     /locus_tag="VFY74_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX5931038.1"
                     /translation="MTEQASDAASQVNDIFDAVEIAGTVGFEEFKHFLDHVPIAVIVS
                     KSVRGEQRIVYANKAFAALTGHDVGKIKGRSWSILEAFRNEDDPQLTLGHALLTDEDS
                     VGTFQLQEPKPALVEVYAGVIQDDEGTEKYRIAALVDVTERERSQREEFARQIRDKDL
                     LLKELQHRVKNNLQLITALIRLEARSQRNGEETAFKRLAGRIESLQFLYHALSATPAG
                     QDIDLGQYLSQIAAGVMRTHAVEGIRLDVKVEIVPVSINVAMPIGLTVNELLTNAFKY
                     AFVGRDSGTITLECLREEPDGCRVIVADDGIGLPPGVTWPADEKLGALILQTLRENAK
                     TELDVTSVPGTGTRIAINFVADSLNLRAA"
     gene            complement(27592..27798)
                     /locus_tag="VFY74_04710"
     CDS             complement(27592..27798)
                     /locus_tag="VFY74_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEX5931039.1"
                     /translation="MPAKSRAQQKAAGAALAAKRGKQKVSALKGASRSMYDSMSEKEL
                     DEMASTKRKGKPEHVSSSTSKRSR"
     gene            28226..28525
                     /locus_tag="VFY74_04715"
     CDS             28226..28525
                     /locus_tag="VFY74_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HEX5931040.1"
                     /translation="MSSTDTPDMRPSAAKRPPSAAGSASRDASDLSDQIDAIRTDLQS
                     LSSTVGRIANRQLSRAQDKVSETAQDAEDAIRRNPLSAVAIAVGLGFLFGVFTRR"
     gene            complement(28649..31048)
                     /locus_tag="VFY74_04720"
     CDS             complement(28649..31048)
                     /locus_tag="VFY74_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5931041.1"
                     /translation="MRADRTDMPPRSRFPVLSAPSDARTAILQGLLIATLVIAALYFG
                     REVLLPLTVAIVLSFVLTRPLVLLRRIGLPRVIAVGIVVSCAFAIIFALGWFMSREIT
                     QLAADLPSYRHALTEKIKGLREGAPSSSVLKKAGDVLSDLQKELNKSGRESPPTPEVG
                     AEAERPADRPMPVEVRQPQPTSVEIFRQIAGTLLPPLATAGIVLLFVVFILLQREDLR
                     DRFIRLLGSSDLQRATTTLNDAATRLSKFFLRQLLINAAFGTFITVALWVIGIPTPLV
                     WGVLAMLMRFVPFIGGYIAAMPPLLLAAVVDPGWSTFLIVLALYVVSEVTMGQVVEPL
                     VFGHGTGVSPIAVVVSTVFWTWLWGPLGLLLAMPITVCLAVLGRNVEDLHVFEVLLGD
                     EPALTPDQSFYQRALSGDAAEATYQAELCLKDQGFVACFENVALAGLKLAERDARRGL
                     LDAEQTERISDTVKEMLDDLADFQPRRWFSKLRQKAEEPDETPTGLASLDAAENEGDE
                     TEAAPEIDRAELAPGWQVDEPVLCIGGRTALDEAAAALLAEVLKKRGLGAAALETETI
                     SAGHIASLAGTEAKFVCLCYLGLGAGPAHTRYLVRRLRRILPEGTAILVAFFGGEGDA
                     NAVKEVLAEAEADAYAASLQEAAEIAIAAAKGELKSEEPGEDDAPTAIRDSATVPASR
                     EPAKPQDHGRERANRSAAPRQKSRRKSDDEHGEGAHLLHGPSDAAVHEVHAFAGPGEH
                     DRLPDPRLLPQGGNDREDKESEREQHAGHDRRGLKPSEPVGGDEGLVSRKESKRRGRQ
                     K"
     gene            complement(31324..32346)
                     /locus_tag="VFY74_04725"
     CDS             complement(31324..32346)
                     /locus_tag="VFY74_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5931042.1"
                     /translation="MNRKPDPYGRDATLPYHIPLKGLWQVSERVWTESNRDNLSVVAA
                     GCAFFALLAIFPVLSAVISVYGLTASPDTVEQHFGILRSVLPPQASDMVMDQIQRVAA
                     ASGGRLGWGLAVSLGLALWSALAGTQAMMAALNIAYEEPERRSLFQYYLNALTFTIIG
                     MVGGVVMLLAIVYVPILFAVAGYSRALELVVGVGRWPFLALFVLFILSLLYRYGPCRR
                     SAKWHWVSIGSLFAMVAWLIASAGFSLYVSHFANYDRVYGSLGAVVILLFWLYMSFYI
                     VLLGAEINAELELQTAEDTTTGEPRPMGKRGAFVADHVAGGPGGEKRPVSPVTRDPAA
                     AKPGGS"
     gene            complement(32445..32603)
                     /locus_tag="VFY74_04730"
     CDS             complement(32445..32603)
                     /locus_tag="VFY74_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX5931043.1"
                     /translation="MDLTTILVIILILILIGVLPTWGHSRAWGYGPSGIIGVILVVVL
                     ILLLLGRL"
     gene            complement(33392..33676)
                     /locus_tag="VFY74_04735"
     CDS             complement(33392..33676)
                     /locus_tag="VFY74_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931044.1"
                     /translation="MTDSSGSGDPTRTRFLRVLLIDAAVLIGILAPTPSLANWWIVRS
                     SDEKCLVVDIEPTPGDEGVTRIGKEAYPTEEEAEADLKRLCRNSEAGFGR"
     gene            34185..35187
                     /locus_tag="VFY74_04740"
                     /pseudo
     CDS             34185..35187
                     /locus_tag="VFY74_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
     gene            35447..35881
                     /locus_tag="VFY74_04745"
     CDS             35447..35881
                     /locus_tag="VFY74_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5931045.1"
                     /translation="MSDWTLDDIAEKLRDIDFAMLLTQSESGEIAARPMSNNREVEYD
                     GDSYFFTLEETDMVAHIERNSKVALSYAGNKGLLGKPPMFIAMRGVAELIRDKDRFAA
                     HWSEDLERWFEDGVDTPGLVLIKVHADRIHYWDGEDDGELEV"
     gene            35990..36214
                     /locus_tag="VFY74_04750"
     CDS             35990..36214
                     /locus_tag="VFY74_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931046.1"
                     /translation="MGNKQRQKSDNPPQKHPEQVEQNPEHRDRDYKREETNIDRSERR
                     PGQQTEKQRRTGERDAVEGEGEAEDESAVS"
     gene            complement(36272..37582)
                     /locus_tag="VFY74_04755"
     CDS             complement(36272..37582)
                     /locus_tag="VFY74_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX5931047.1"
                     /translation="MRFLGIGDAADLGSLYLRLAAEGHEVKIYIGNPLCRDTLLGLVP
                     HVEDWQNELGWVREAGSEGCLLFENVGSGRGELQDRLRADGFNVIGSSAYGARLENDR
                     TYAQNVLADLGLPTAATFTFSQIEEARRFIAERPARYVVKSNGPDAATFVGSRPDGAD
                     VRSVLSSDPNLATASFVLMEFVEGIEMGVGAYFNGVEFLMPACLDWEHKRFFPHDMGE
                     LTGEMGTVVTYSGGKTFFERTLGKMRPLLEQNGYCGYINLNTIVNAQGIWPLEFTCRF
                     GYPGYAILDPLQRTGWGALFRAMLDRSATRFEVEPGFCVGIVMTTPPFPYSREHVLEP
                     KGLPISFKGELSTAERANLHYGEVGLSDGVLVTSGANGYTLVVTGTGDTITAARDAAH
                     ELAGKLVIPNARYRRDIGQKLIDGDFAKVQSWGLIDPQSIISSH"
     gene            complement(37600..38484)
                     /locus_tag="VFY74_04760"
     CDS             complement(37600..38484)
                     /locus_tag="VFY74_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5931048.1"
                     /translation="MSGPGFERFQTELSKYNGQQLGPRFPHQAWRDELKTYAEVLRAE
                     GEYIEAVRDEISPLLADVPSDVDDFIDWFEDLKETGPGQGDPLFPWLAEAATRDEMLW
                     FLTQEVAGEAGFDDLLALTQVKMPVTAKLEMARNYWDEMGRGRDTAMHGPLLERLASY
                     LDIDARPERVVPESLALGNAMLAMARSRRYCFQSIGALGAIEMTAPTRAGFVDAGLKR
                     LRVPTKKRLYFTLHAVLDIKHSECWNREVLRSLVAEDPRCAAAIGEGAVIRLWHGARC
                     FERYRAHFGLDVRQSQAA"
     gene            complement(38532..39278)
                     /locus_tag="VFY74_04765"
     CDS             complement(38532..39278)
                     /locus_tag="VFY74_04765"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5931049.1"
                     /translation="MFELLSEADALAPDNGRYRSRVRFATIGDMVFAHSAFPTIEPDA
                     VFFGPDTYRFVRLVRDSVADVASDRRLRLVDVGSGSGAGGLYAARCLPGEAEILLADI
                     NRKALVFSAVNAAINDVAHARVLHSDILDGLEGEADLIIANPPYLVDEERRLYRHGGG
                     DLGVAVAARIAAEGLARLAAGGRLILYTGTPIIAGGDPFFDSVEPFLKLYCSQFRYEE
                     VDPDVFGEELEKPSYAGVDRIAVVSLVARK"
     gene            complement(39504..41381)
                     /locus_tag="VFY74_04770"
     CDS             complement(39504..41381)
                     /locus_tag="VFY74_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5931050.1"
                     /translation="MTAVEKLNILLVDDQPSKLLSYEVILQDLGENLLKASSGTEALS
                     QLLQNEIAVVLVDVCMPDLDGFELAAMIRDHPRYQKTAIIFISAINISDVDRLRGYEM
                     GAVDYVPVPVVPAVLRAKVKIFAELYRKTRQLEALNRELEIRVSERTAELESYVHKLA
                     ESEQRRNLALAAGAMGSWDWDLINGDWMWDEGQCRIFGVDPQTFKPTLERVMSLVEPQ
                     DAERLQEMISDFSDGAKAYQTEFRVRRPDGERRWCFGTAAPSADEAGRLVRVSGVTID
                     ITDRKEAEERQAFLAKEVDHRARNVLAIVQSMLRLTKAESAEAYAAVVEGRIRALSRA
                     HMLLSESRWEGADLKRMLVEELDPYRAGDRVTINDDGPAVLLDAKRAQTVALGVHELA
                     TNAAKYGALSNAAGKLSVSWGIGANGLTIEWLEQGGPEVVPPKLKGYGTRVIKASLEQ
                     LGGRADFNWRRDGLLCQLLLPLGEMAEHRNRGGGQKLSNMPAKSSLEIDKGSAVMLVE
                     DESIVAMMMAEALTTLGFKVVGPCTNLGDAMSAAARQPIAAAILDIRLGEELVYPLAD
                     DLAARGIPFVFVSGYGLESLNPRYRTTPVLQKPVDVEALRNLFAVPAGETAAWRTASR
                     Y"
     gene            complement(41396..47578)
                     /locus_tag="VFY74_04775"
     CDS             complement(41396..47578)
                     /locus_tag="VFY74_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5931051.1"
                     /translation="MRVGDFAVRMPSDRSGIEGKIADAFNEIVGANQRLANQLERVGQ
                     VVGREGETRKRVKLGVSHGAWGEMETSINTLIDDLLWPTTEVTRAIAAVAQGDLLQTM
                     RLDVDGRPLRGEFLHSATIVNTMIKQLGLFTSEVTRVAREVGTEGKLGGQADVPEVTG
                     VWKDLTERVNSMASNLTAQVRNIADVTIAVANGDLSKKITVDVRGEILQLKEAINTMV
                     DQLRSFASEVTRVAREVGTDGKLGGQAIVPGVAGTWKDLTDSVNAMCGNLTAQVRSIA
                     QVTTAVARGDLSRKITVDVRGEILELKNTINTMVDQLNAFASEVTRVAREVGTEGKLG
                     GQAAVPGVAGTWKDLTDNVNFMASNLTAQVRNIAEVATAIAGGDLSKKITVNVSGEIL
                     QLKETINTMVDQLNAFAGEVTRVAREVGTEGRLGGQANVLGVAGTWKDLTDSVNSMAS
                     NLTAQVRNIAEVATAIASGDLSKQITVDVRGELLELKDTINTMVDQLNAFAGEVTRVA
                     REVGTEGKLGGQAMVRGVAGTWKDLTDSVNSMASNLTAQVRNIAEVATAVAEGDLSKK
                     ITVDVSGEILQLKETINTMVDQLNAFAAEVTRVAREVGTDGKLGGQAEVPGVAGTWKD
                     LTDSVNSMAGNLTGQVRNIAEVATAIANGDLSRKITVDVRGEILQLKETINTMVDQLN
                     AFAAEVTRVAREVGTEGRLGGQANVPGVAGTWEDLTDSVNSMAGNLTSQVRNIAEVTT
                     AVARGDLSRKITVDVKGEILELKNTINTMVDQLNAFASEVTRVAREVGTEGKLGGQAQ
                     VPEVAGTWADLTDNVNFMAGNLTAQVRNIAEVATAIASGDLSKKITVDVRGEILLLKE
                     TLNTMVDQLRSFAGEVTRVAREVGTEGRLGGQANVPGVAGTWKDLTDNVNLLAANLTT
                     QVRNIAEVTTAVARGDLSRKITVDVKGEILELKDTINTMVDQLNAFASEVTRVAREVG
                     TEGKLGGQAQVPGVAGTWKDLTDTVNVMAANLTEQVRGIVKVVTAVANGDLKQKLTVK
                     SKGEVAALAETINNMMDTLAIFADQVTTVAREVGVEGRLGGQANVPGTAGTWKDLTGN
                     VNLLAANLTNQVRAIAEVATAVTQGNLTRSIQVDARGEVAELKDNINTMIVNLRLTTD
                     RNTEQDWLKTNLAKFTNMLQGQRDLATVGRLLLSELAPLVNAQLGVIYQVDGSGDEPS
                     LKLLSSYADDDVNGHPPLLRFGKGLIGQCAADARSILISQMSDNAVTIGSALFKAAPR
                     NVIVLPILFENQVKAVIELASVEEFTDLEVTFLEQLTGGIGIVLNSIEATMQTESLLK
                     QSQQLATELQTQQRELQQTNDQLGQKAQQLAERNVEVERKNQEIEQARRALEEKAGEL
                     ALTSKYKSEFLANMSHELRTPLNSILILGQQLSENPEGNLAPRQVEFARTIYAAGTDL
                     LNLISDILDLSKIESGTVSVDAQQISFSSLVEAVARPFRHEAEARDLSFNVELDPNLG
                     HSITTDSKRLQQVLKNLLSNAVKFTEQGGVWLKVYPVTEGWSADHPILSQVSSVVAFE
                     VSDSGIGIPLDKQKIIFEAFQQADASTSRKYGGTGLGLAISRELSNLLGGEIGSKSTP
                     GMGSTFTLYLPMVYAGPVVPARPAPAGVGGPQMARPLMPTVVYEHSVERIPDDRDDIE
                     PGDAVLLIIEDDPHYARVLSDLARENGFKVVIAMRGREALELALEFRPTAISLDVFLP
                     DMLGWTVLSQLKQNPITRHIPVQILTMEEDRQHGLTRGAYSFLTKPASTESLESALSR
                     IKDFAEPHKKRLLVIEDNEAEQMSIAELLGHDDIEVVTTGTGAEALDLLNEERFDCVV
                     LDLRLPDMSGFDLLKELRADDSLSDIPVIVFTGRELSAEEDAQLHTMARSIVVKGVES
                     PERLLDETALFLHRLVTKLPPDKQQMIERLHRSDEHLKGKTALLIDDDARNIFALSSM
                     LERRGMRVLTAVNGREAIQLVKSTPDLAIVLMDIMMPEMDGYETIAAIREDPAFRRLP
                     IIALTAKAMKGDREKSLEAGASEYIAKPVNTEQLLSAMRSWLHR"
     gene            complement(48064..48249)
                     /locus_tag="VFY74_04780"
     CDS             complement(48064..48249)
                     /locus_tag="VFY74_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931052.1"
                     /translation="MRARYPLEEMALLLGVTRETYTKYEGRGASLMPHNLIVPFCLAC
                     RVSVEWLLTGIGPTRSR"
     gene            48677..50380
                     /locus_tag="VFY74_04785"
     CDS             48677..50380
                     /locus_tag="VFY74_04785"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5931053.1"
                     /translation="MLFIALAVDYDGTLAHDGRVDDAAMAALEKVKKSGRKLILVTGR
                     EIADLQRVFSGIEIFDLVVAENGALLFDPAQKKEEPLSPPPSDRFVALLRSRKVAPLS
                     VGRSIVATWEPNETIVLEAIRDLGLELHIIFNKGAVMVLPADINKASGLKEALKRLSL
                     SRYNVVGIGDAENDQAFLSACGCAVAVANALPSVKEKADFVVADHGAGVAELAEILTG
                     TDLAGHNTGLGKIQPVIGEDAGGKLRQLKPLDTVLVTGSSGGGKSTVVTALMEQMIDA
                     NLQFCVVDPEGDYAEFPAVAVGDAKHEPRIPEVMDLLAKPDTSVVVNLLAIDPGERPR
                     YLAGLLPELSKLRIETGRPHWIVLDEVHHCLPANWDPAPVSLPQELPAAIAVTVHPEE
                     VAAEFLKMVTTVVGVGEQAKKMIETFCGARGEACGTFPERPDAKHGLVWTATGGVQAV
                     ALRRPKVRQKRHIRKYAQGELGEDRSFYFRGPQGALNLRAQNLAIFLQLAEGVDDQTW
                     MHHLRAGEYSQWFDQAIKDEELAAEARIVEQDETLSVTETRTQIKKLVENKYTAPAKS
                     S"
     gene            complement(50831..51877)
                     /locus_tag="VFY74_04790"
     CDS             complement(50831..51877)
                     /locus_tag="VFY74_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX5931054.1"
                     /translation="MPREVKTLCSRYGLTYVETADLRLRRRRCGRGFGYIDGEGRTVR
                     DKSLKARIRQLAIPPAWTEVCIAEDDRAHIQAVGRDVEGRLQYRYHADWENARAETKL
                     HRLRRLGSALPRLRDTVRKALSAPELTRNNVIAAVVRLIDRALLRPGHDEYAQATGSR
                     GASTLLKSDVAVTGDKVVVEFKGKAGKPVRREVKDRLLARVTRRLLAGKGRRLFSAVD
                     DNGRVRPVTAREVNAFLAEASGTDVTAKDFRTFRASAEALAFLTERNGHETERLKKQT
                     IVQAADRASEILVNTRSVARSSYIHPVVIKAYESGKLGASLFRGRVRAGLNRMESALM
                     RFLETSTSFRNDRK"
     gene            complement(51990..52691)
                     /locus_tag="VFY74_04795"
     CDS             complement(51990..52691)
                     /locus_tag="VFY74_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5931055.1"
                     /translation="MKWVVLFLAFCGALLYVAGAFTPSPVEAPAPAKNSPLNGPEQAQ
                     RSSRPQSNLHAIDPAGPSTETGGLSVVEEGAGDAGQGYAPESQQDLQDSAPNGTASTQ
                     APSSLGTEQQATPAWGRLLRGAPVHSGPSVSSSRLGYATAGAEMRLLERNLGWVRIRD
                     PATSREGWIYEEHIAATEEPSVPGYGEDQAALADDDETGALEQQERSATAKKSKKKYS
                     KKRWRKRLRFVFRRF"
     gene            complement(52849..53097)
                     /locus_tag="VFY74_04800"
     CDS             complement(52849..53097)
                     /locus_tag="VFY74_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931056.1"
                     /translation="MPYFSGVLTGIALTILVVFLVDHVGPDSDKRDIVNWDYVGASLG
                     ASFEKVGEEVRQEVHEATAPEGEKASPPAAPAPATGQQ"
     gene            complement(53251..53430)
                     /locus_tag="VFY74_04805"
                     /pseudo
     CDS             complement(53251..53430)
                     /locus_tag="VFY74_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
     gene            complement(53466..53636)
                     /locus_tag="VFY74_04810"
     CDS             complement(53466..53636)
                     /locus_tag="VFY74_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX5931057.1"
                     /translation="MLYWALVFLIIALVAGALGFGGIAGASAGIAKILFFVFLVLLLI
                     SLVTHFVRGSAP"
     gene            54012..54506
                     /locus_tag="VFY74_04815"
     CDS             54012..54506
                     /locus_tag="VFY74_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5931058.1"
                     /translation="MNKQSHLDRVWEVVGKVGVCMMTTHFADGLRARPLEARADRDEN
                     VIWFLSDRRGAKDDEIEAYPSICLTFIDPKEKVYLSISGTASAARDPERAKNLWNKEQ
                     QAWWPKGPSDPNVLVIAFRPQRAEIWDGPASSAVASYEFAKARATGTKPNLGENRKST
                     VKMD"
BASE COUNT        10449 a        17091 c        16936 g        10433 t
ORIGIN      
        1 agagagccgc aagctgttcg acgcggcgct atcggggaat ctccccgccg cctaacccac
       61 cacgatgtgg ttgcggtcgt ggtgctgcgt caaaacaaaa aggggcgccg gtttaagccg
      121 gcgcccaatg cagcacgcag ctacatgttt acggtgctac atcttcttct cttcgcattt
      181 gcttgtgttg gcgtcccagg tgccgccggc cttctcgcag tcggccgcat tcgaggcctt
      241 cgacacgtcg cccgcgagag cggggccggc gaaaccaagc gcgagagcca gagcaaaggt
      301 tgaaatcagg gtcttcatca cgcgttactc ctctgttggt gcaggggtga gcggacaacg
      361 ccgcctcgtc cggatcgttc cggacttttt ttcaacgcgc ttcgagatgc aggcgtggac
      421 agagacgcca agctggaggg cgttgaaaca ggactttggc aagaagtgtg gggaactttg
      481 ttcgcatcat cgcgttcagg gcgatgaaac gtcggaccta aacctgtcct cttcagtgcg
      541 acggagattt aggccggctc ggcatcaatc ctcgccggaa catttcgcca gtctttgtcc
      601 ccctacgaaa ggagtcatca atgcgtggtg tcatactttg gctgctcggt gtcccgatca
      661 cgatcatcat tcttctttat ctgttcaaca ttctatagcg gcaataacgg cgctcttttc
      721 gctggcgatc gtctcggtca gccgtttcca attggacgcg gacaagtcgg gaaccgtcca
      781 gccggttgaa cgttgacctc aatatgcttt ggctgaccgc ggtgggaagc ctccaacaag
      841 caggagtcac gtgatgaact gggatcgtat cgaaggcaat tggaagcagt tcactgggcg
      901 ggtcaagcag cagtggggca agctcactga tgatgacctg cagcaagtcg aaggccgtcg
      961 cgatcagctc gtcggcaaga tccaggagcg gtatggcatc gccaaggacg aagccgagag
     1021 gcaaatcgat agctggctga ccaacgtcta gcggaaatgc ctacggcgcc gggcaattcc
     1081 ccggcgccgt taattctcct gccgacttat cccacctcaa cgacttatcc cacctctatg
     1141 aggtcgcaac cgtgaccacc attcttatca ttctcctcat actcatcctc atcggggcct
     1201 tgccgacttg gccgcacagc cgcggctggg gttatggacc gaccggtatc gtcggcatat
     1261 tgctcatcgt cttgctggtc ctgatgttgg cgggccgcgt gtgagatcga gcggctttcc
     1321 gatttgtctc gttcggaact gaaggagagc agagtgccgc gctggctgac ccgcaccgaa
     1381 gcccgccaag gcgcaggccc aagagcaatg ctctgggttc tgctcgcttc tcttgcgctc
     1441 gcctgcgccg cgggattggc actcgggctc ggatggatca ctctgccctg gaccggctag
     1501 tcgcgctgcg ataaaggcgg catcatcaca gggagaagcc atctcggacc ctccggagtt
     1561 ctcgctcggc atggatcagg cattagggct tcccgaagcc atcttggtcg cggatctcgg
     1621 cggaacgaat gcgcgtttcg ccatcgccga gctcgcaagc ctcacgctct cgcacgtgat
     1681 acagtttcct tgccaaatgc atcgcgggcc agaagcggcg atccgcgcgt atctcgagca
     1741 ggtcgacgcc acgccgaaat acgcgtccat cgccattgcc gcgcccgtca cgggtgacga
     1801 cgtcgtcttc acgaactcgt actggtcctt caagatcggc gcgctttgcc gcgacctcgg
     1861 cttcaacgac gttcgtgtgc tgaacgactt cgaggcgttc gcgctggctt tacccagtct
     1921 ggggtcgcag gaagtgctcc agatcggcgg caccgagccc caaatgcggg gcaccaaggt
     1981 cgcaatcggc gttggaaccg ggacgggcat ggcggcgctc gtttggagcc cggctggatg
     2041 ggtcgccgtg ccgagcgagg gcggccacgc gtccttcgcg tgtcaaaccg cagaggactt
     2101 tgcgcttgcc gaggagctcc gtatggaccg gtcccacctc tccgccgagc ggatcctctc
     2161 ggggcccggc gtcagcgcct tgcaccaggc tgttgccgca ttgcacggcg tggaggtcga
     2221 agcccttgag ccaaacgacg tgctgacgcg tgggctcgcc ggagccgacg ctcttgccgt
     2281 cgaggccttg cgctttttcg tgcgatgcct ggggcggttc gccggcgatg ccgcactgac
     2341 ttttggcgcc cgcggaggcg tttatctcgg cggcggcatt acgcccagaa ttctcgacct
     2401 cctaaaggcg ggaccgttcc gcgacgcctt cgaggacaag ggcagaatga gaggctatct
     2461 tgctccgatt ccgatttatg ccgtgcttgc cgaacacgcg acccttcgcg gtgccgccat
     2521 cggcgcaagg atgaatttgg cgtcgtcaag cgcatttccg ctcggcctgt cttaggcggc
     2581 tccgagagcg ggtgccatga gcgacttcgg actcgatctt gcggtcatcg gcaattgcca
     2641 cacggccgct ttgctcgaac ccacatcgcg tcttgtctgg tggtgctatc cgcgtttcga
     2701 cggtgacccc gtcttttcgc gtcttctcgc cggcgacgat gaaaagggct tttgcgacgt
     2761 cgtgctcgaa aacatggtgg acttttcgtc cggctacgag cgcaacacgg cgatcgtttc
     2821 caccgtcctc accgacgcgc aaggcgcagc cgtcaagatc accgatttcg cgccgcgctt
     2881 tcgccaatac ggccgggttt tccgtcctgc ccaactcatt cgcatcatcg agccgatcac
     2941 cggactgcca cgaatcgcca ttcgcgtgcg tccaaccagc gactacggca aaccaatcga
     3001 gaccagcgcg ttgcgcagca gccatatcag ctatcagggc aagaacgcgg cgatcaggct
     3061 caccaccgac ggtcccctgt cctacatcga tcaggagtcg ccgttcgctc tgaccaaacc
     3121 cattcacatg gtcctcgggc ctgacgagcc tttcgccgag gacattgcca cgacgtgccg
     3181 cgagtttgcc gatcgcacgc acgacttctg gatggattgg gtccgccgcc tctccatcgc
     3241 ttacgattgg caagacccca ttatccgctc cgcaatcacg ctcaaactct ccaatttcga
     3301 ggagaccggc gccatcgtcg cggcgctgac gacgtccatt ccggaagcct cgggcgccgg
     3361 gcgcaactgg gattaccgct actgttggct tcgagacgcc tatttcgtgg tgaaggcgct
     3421 caatcgcctc ggcgccaccg aaacgatgga aagtttcatt tccttcattc tcggcatcgc
     3481 ggcgcaggat cccttgcggc ccgtctacgg catcgtgccg accgaacctc tcgacgaggt
     3541 catcgcgccg agcctgaagg gctatcgcag cgacgggccg gtccgcatcg gcaacgccgc
     3601 ggcgttacag catcaaaacg atgccttcgg cagcattgtg ctcgcggcca tgccgatgtt
     3661 ttttgaccgg cgcttgcccc ggcccggcaa cgaaggtctg ttccattttc tcgagtctct
     3721 cggttcgagg gcagccgagg cagcattcac tcccgacgcc ggcatctggg aatatcgcag
     3781 ccgcgcgcac gtgcatactt actcggcggc aatgtgctgg gcggcggcga gccggcttgc
     3841 ggcgattgcg gcccatttgg gcttgatcga ccgcgctacc cattgggccg atacggcgag
     3901 caagattcag gacgctctgc tggagcaggc ttggaacgag aagcgacagg cctttacttc
     3961 cgcgtttgga tcggagcatc tcgatgcgag cgtcctgctc ttgccggata tcgggctgat
     4021 cgaaccgacc gatccgcgtt tcacgcagac cgtagccgcg gtggaaaaag acctgctccg
     4081 cggcaaacac gtgctgcgat acgcaaacga cgacgatttc ggcatgcctg aagtcgcttt
     4141 tctcgcctgc cgctattggc tcatcgacgc atggtggtcg ctggggcggc gcgaggaggc
     4201 acgcgaactg ttcatcgact ctctgcgtta tcgcaatagg tatgggcttt tggccgaaga
     4261 catacacccc gccacaggcg aactttgggg caattttccc caaacctact ccatggcggg
     4321 acttattctg acggcgatgc ggctgtcccg gagttgggag gatcgctatt ggcgcggcta
     4381 gttctcgttt ccaaccgcac gggccttcca cagggcgacg gcacgcagcg tgccggcggg
     4441 ctcgaagtcg cgcttgcgcc gattctcaca gggatttcaa cgctttggtt cggctggagt
     4501 ggcaaggtcg tcccgcccga aaaggtcagg accgagacgg tcgatcgcaa cgatgcaacc
     4561 tatgtcgtca ccgacctggc ccaggacgac tacgaagaat actacaatgg atttgccaac
     4621 cgcgttctgt ggccagtttt gcattaccac atcgatctga ccgaattcac gcggcgcgac
     4681 ctcagcggct attttcgcgt gaaccgccat ttcgccaacg agctctccaa aattctccaa
     4741 ccggacgatc ttgtgtgggt ccacgactat catttgatcc caattgccga ggagctgcgg
     4801 cagcggggac acgacaacgc gctcggattt ttccttcacg tgccgtttcc cgcgccagag
     4861 gtgatgacgg tgcttcccaa tcatgagcag ctgcttccgc tccttctgca atacgacctc
     4921 gtcggcctcc aaacggaaac cgacgtcgga aactttgtgc gctacctcat cgccgagcgt
     4981 cgcggttcgg accgcagagt catcgaggca actggcggtc aattcctgac cggcggtgaa
     5041 ttcgcgttct cctcgcgcgg ccgccagacg agaatcggca gttttcccgt cggcattgat
     5101 ggcgcggagt tcgagcggct cgcccgtcgc gctgtgaagt cgactttcgt gcgtgacgtg
     5161 gtcgagagcc tcagcgcgcg cgcgcttttg atcggcgtcg atcgtctcga ttactcgaag
     5221 ggtctggttc agcggatgga tgcgatcgaa cgatttctcg acatgtatcc cgcatggcgc
     5281 aacaagatca cttacctcca gattgccccg aagagccgat ccgagattcc cacttatgcg
     5341 gagctcgagc aggcggtcgg ggcagcggtc gggcggatca acggcaggta tggcgaggtc
     5401 gcctggacac ccattcgcta cctcaaccgc gcgttcagtc gttcgaacct ggcaggatta
     5461 taccgctcgg cgcgcgtcgc catcgtcacg ccgctccgcg atggcatgaa cctcgtggcg
     5521 aaagaatata tcgcggcgca ggacaccgag gatccgggtg tgctcgtcct ttcccgcttc
     5581 gccggagctg cggtcgaatg cgaggctgcg gtgctcgtca atccctacga tccggaatcg
     5641 gtggctcgcg ccattgccca ggcgctcgac atggctttgc ccgaacgccg caagcgccac
     5701 gaggcaaacc tggcggcgct cgcacgcaat cgccacaatg aatggagcac gcgcttccta
     5761 caatcgcttc ggaacgcacc caaggccgcg aatcgccccg cggcggccgg ccgcctggat
     5821 gcgctgttct aattccagtc agtcgtcgga ctcggtcttg acatcgacgt gaccccaagt
     5881 cctgacgacc tccaacgccg ttcgctcgcc gtagcgccgg atcaggacgt cgacggcgcc
     5941 ctctcccgcc ctcaaccctc tcagcgagac ctcgtccaga aactttggaa gcgtcggctt
     6001 gacgaagcgg atctcgcgct tccagggatc ctggacgatg cccaagctcg cctgaagcag
     6061 agcgaagaac gaacccgcag accaggcctg cggcgcgcag gcgaccgggt agctcgtcgg
     6121 tccgcggcca gccagacgcg gaaagccgca gaagagctcc ggcagtcgct gcaaatcgag
     6181 gtagctcgca gcctcgaaaa gagcgcgaaa aatcctcgtc acttcgccct tggcgcggta
     6241 gcgcgcgaac ccgagcgcga tcagcgcgtt gtcgtgaggc cagaccgagc cgttgtgata
     6301 ggacattggg ttgtaccgcg gctcggcggt ggatatcgtc ctgatccccc agccggaaaa
     6361 actgtcgggc tgcatcagtc ccgaggctac cttccgcgcc ctgtccggtc tggcgatacc
     6421 gctcaacaga acttgaccgg cgttggagga acgtatgcgg cacggcacct tgtcgccgtc
     6481 gagcgccaag gcgtaagtgc cgagttcccc gcaccagaaa gagtcttcga aacgttcggc
     6541 aagaaggtcg gcctcgcttc ccagctgccg agcccggtcc gggagattca gggtttcggc
     6601 gcaccaggcg gcaagcctct tggcggcata aacatagcct tgaacctctg ccacggcgat
     6661 cggccctgtc gcgagccggc catcggagtg gaaaatcgag tcctgagaat ccttccaacc
     6721 ctgattgatc agccccttgt cggtctgccg gtcatattcc acgaagccgt cacgatccgg
     6781 atcgccaggc ccatcgatcc atgcgagcgc cctttcgatc gcgggccaga gctcttgcag
     6841 cgtggcgaca tcgccggtcg actgcacata ttgtcccgcc agcaagacga agagcggcgt
     6901 ggaatcgacg ctgccatagt agcggccgaa gggcacctcc ttcagattgg ccatctcgcc
     6961 cgcgcgcatc tcgtgcagaa tcttgccggg cgcggcgtcg gaagcggcat cgaacgcggt
     7021 tgcttgcgtg gcagccaacc gcctcagcac ggcaatcgga atgcggcggt cgagccacag
     7081 gcattgaagc gcggtgatca gtccatcgcg gccgaaagtg gtcgaaaacc aagggatgcc
     7141 ggcataaggg taataccctt ccggcgtttc ggtgagcagc attgcgaggt cggccgtcgc
     7201 ccgccggagc atctcgttga aactgtgatt tgaggtgtgg attttcgttt ccaccctcat
     7261 cagcttcttt cgctcaagat gcgcgcgccg catggttctc aagaacagcg tctcttcgga
     7321 gcgcgagtcg caggacactc gcgtgtagag agacgcgacg ctgtgcgggc tcagatcaag
     7381 ccggtaggac gcacaatctt cggtcagcgt caaaggcgtg gggtcgaagg cgatatgggt
     7441 ccgccgcatc accccgtccc ggcccgcata agtcagagtc gcgcgggttt cgctgatttc
     7501 ggcgcgctcg accccgcgcc ggcgccgaac gacgccccgg acttcgaaaa catcggcgaa
     7561 gtcgctctcg aaggcgagcg acagcaggag ctgaaccgaa tccgctccat aattgtgcaa
     7621 ggtcaggcgc tggtgaagcg tctcacgcga gatgaacaac gtgcgcgcga tgtgcagcag
     7681 gttcttggac agaaccacgc tgtcgcgatc gcggaaatcg ggattggtca ggtcgactct
     7741 taagacacaa ttgtcgttcg atagcgtcga tccgagaagg agaggcgcga cgccattgat
     7801 gagcaactca aagcgggaaa ggtagcgcgt gtcgcggtaa aacaggccgt cgggtccacc
     7861 gactgagacc cccacgtcgc catggctgtc gaggacgagg aaactgtcgt cctgcttcat
     7921 cgtgtagcgc ggtcgcgcgg acgacgatgt gaccgcaatg tcgaacagcg gttccggctt
     7981 aggctcgagc acttcttacc aagcgtcccc gacgcttatt cggcggcata gattctcgca
     8041 tgtaccgcat cgcccgtcag attgatagtc tgaccggaga cgggctggcg aggctggcca
     8101 acgcgggatg gccgcacaag cgactgatac acccgcagat aatcgttggc catgcggcga
     8161 gccgagaacc gcttctcaaa gcgctggcgc actttacgac gatcgagctt gacgatttgc
     8221 ggcaacttgg cgactgcctc ctcgaatgtc gtgacgaccc ggccggtgac cccgtcgtcg
     8281 atgacttcag gcacggagcc ccaatcgaaa gcgaggacgg gcgtaccgca ggccatcgcc
     8341 tcgatcatca cgaggccgaa aggttccggc cagcagattg gaaagagcag cgccgacgca
     8401 ttgcgcagaa agtgaccctt ttccttttct ccgagttcgc cgacgaactc gataccgggg
     8461 agacctagca gtggtttcac ttcctcgcgg aaataatgct cgtcggcgcg atcgaccttg
     8521 gccgcgattt tcaactccat gttcaattcg cgcgcaatgc ggatggcctg aaccggatcc
     8581 ttttcgggcg aaatgcgtcc gagaaacgca agatagcccc ctcgcggatc aaattcggcg
     8641 cgatggagat cgaggggcag gccatgatgt atcgtcgcaa tgaaatttgc gtaagagatt
     8701 ggcgcgcgtt gtgcgtgcga gatggacaca agcggagtgt tgcggaaata gcgataaaag
     8761 ggagcgttcg ccggcagatc ctgtcggcca tgcaacgtcg tgacggcgcg gctctccaag
     8821 cccccaatct gcgggaaatg cagatagtcg atatggaagt gcaacacgtc gaagtgatgc
     8881 gccacccgca tgagcttgtg cagcatgacg atgtaatgag gaatggggtc atgagcctcg
     8941 gcttccagcc gcagcgccga cttgcagcag ggtacgaagg cggcttcggt gatcgaatcg
     9001 ccgctcgcga ataaggtgac gtcgtgaccg aggccgacaa gctcttccgt gagataagac
     9061 accacgcgct ccgttccccc atagagccga ggcggcacgc tttccatcaa aggcgcaatt
     9121 tgtgcgattt tcatgcggtc attccgggga tcttggcgaa ccgggatctt ggcgaattcg
     9181 cggggcgcgg cgtcgcctct agtataagac tcaggcggcc gcaaagttcc aaggtgcgca
     9241 gagagaaggc gaccccttcc gtttcgcgcg ccgcgtcgtc gtaggcgcgc tttcctgggt
     9301 caatcgacac cgaattgcgg agcgggcgcg gaacccgccg cgtgaccggt ggttacgctg
     9361 catcaaaacc ggacatgaaa ggaccgtgtc aatgacgaac gaacggaaca gagatcgcga
     9421 cacaccaggc ggcggcggcc gtccggacga gcgcgacgat cctgaccggc ccggctctcg
     9481 cccggagaag cgccttcccg gccaggatca gcaggaccgg ggatcgccgg cggatcgagg
     9541 agccgcatcg cccgggtcag ggtcgacgtg acagcgatca ggacgccgcc aacgaacagg
     9601 acgacgacga cggtcgcgct tagttggaag agcccgcgca agcgggcttt ttcatgaacc
     9661 ccgaaccgag tgagcaggtc tccgctggcc cccgagcagg aacatttttg gcttcaggca
     9721 attagtgtcg agggttgccg ccggcagcaa agctaactgg aacacaccga tgcgcgtcct
     9781 tggcgatgcg tgtccgcaat cggagaaaac agatgggcct ctttagcaaa gacatcaaat
     9841 ccatggacga cctcttcctg cataccctgc aggatatctt ttacgccgag aagcagatcg
     9901 tgaaatcgtt gccgaagttg atcgagaagg cgaccaaccg cgagctcgcc tccggccttc
     9961 gcaatcacct gcgcgagacc gaaggccaag tcgagcggtt ggagcaagtg ttcacgaaac
    10021 tcggccatac accgagaggc gtcgattgtc cggccatcga cggcctgatc aaggaggccg
    10081 acgaggtcgc tggtgaagtc gacgacacca aggtgctcga tgccgccatc atcggcagtg
    10141 cgcaagccgt cgagcactat gagatcgcgc gctacggcac tctgatcgcc tgggcgcagg
    10201 aactcggtca tgccaacgtc atcaacctcc tcaaggccaa tctcaaagaa gagaaggccg
    10261 cgaacctaaa gctcaacgcc ctggccgaag gaaccgtcaa tcgcagagca acagggcgta
    10321 ccgccgccgg ccgccccgga gcggaatcgc gcgcgggctc gacccgggcg gcggcacagg
    10381 ggttgacaag gaaacgcacg ggcaaggtga agcggacccg ccgcaagacg aaatagcccg
    10441 tgcggcgcgc caacgctgcg tcccgaatgc agcgcaattt gatgaccgca gcgtcatttc
    10501 aacgatggcg ggttgtggag agtacgggcc tgccatcccg atggacgatt gaaacagtcc
    10561 ctgcgcaaag agtcaggggt ctagatgggt tcccctcctc ccgcccacct ctgactcggc
    10621 cgaggcccgg ttcgacaaga gccgggcctc tctgccgaag ggcggcacaa agacggtttc
    10681 ggaaagcggg agccgctccc cctagcctcg agaatagccg ccgatgatcg gaagcacttc
    10741 gccggtgatg tagctcgagc aggagggcgc cgcgaggaac acgaaggcgg gcgcgatttc
    10801 ttcgggctgg gccggccgct tcatcggcgt ggagctgccg aattcggcga catcctcggc
    10861 cgattggtcg gcaggattca gaggcgtcca taccgggcct ggggcgacgg cgttgacccg
    10921 gatgccgcga tcgacgagat gagtcgcgag cgagcgcgtg aaggcgtgaa ttcccccttt
    10981 ggtcatcgaa tagtcgagca gatccttgct gcccaggatg ccggtcacgg accccgtcat
    11041 gaggatcgcg cttccctcct tcatgtgcgg caccgccgcc ttcgtcatgt agaaatagcc
    11101 atagagattg gtcttgatgg tctggtcgaa ttgctcatcg ctcagatcct cgaggcttgt
    11161 cacatgctgc tggaaggctg cgttgttcac caggatatcg agctggccaa actctgccag
    11221 cgtctgcttg accgcgtgtt ggcaaaactt cgggtcggcg acgtcgcccg agagcatgag
    11281 acaacggcgc ccctctttct ccactgagcg cttcgtttcc tccgcgtccg catgctcttc
    11341 gagataggcg atcgcgatgt cggctccctc gcgtgcgtaa agcaccgcga cagaacggcc
    11401 gatcccggag tcgcctccgg tgatcagagc gacttttccc tcaagcttct cggacccttt
    11461 gtaatggggc gcgtcataca ttggctcagg atcaaggtcg gcctcagagc ccggcttctt
    11521 gagatgctgc ttgggaagcg gcggaaccgg atagtcgcgg gtgccggcct gcatggcatc
    11581 ctgctcttcc tcgccttcag tcttgggctc gtcggaacga ttggcctctc gttgaatttg
    11641 ccgctgatgc cgtgccgtgg cgcgcgcttc gcgttcgaac gacccgcgtg aggctgattt
    11701 tctcggcgat cgcttggaca tggaagttgc tcctgagaat tttctgcgtg gaccggccgc
    11761 gagaagttga acccgcatcg atcgatggcg tctccgcgaa gacccgtgtc gcgagcgctc
    11821 tgccgacggg gtgcaacgct cagcgagcgc aaatttgcgc gatccccgcg ctgcgtgaac
    11881 gcgcgattgg atcgcgtcgc cgcactaacc ggccggccgc aaaaacgttc cagtgcccag
    11941 agtgattttc gcagcgggga ggaaccggct ttcgcctctt tcgttacgcc agcaatgcct
    12001 ctcctggcag ttgtcccaca cgctccgcgt cctgcggact ttggaaagga accttgatga
    12061 tgagaagagc cctcttggcg ggcgcggtcg cgttggccgt ccccctgctg tccggatcgc
    12121 cagtgctcaa tccggcagct ctggctgccc cggcggcctc gaccgagtca ttcgtccaga
    12181 ccgtcgccat gagcgacatg ttcgaagttc aatccggtaa gctcgcggcc gaccaggccg
    12241 ggaatgcgaa ggtcaagtcc ttcggtcaga agatggtcga tgaccatacc aagacgacga
    12301 atgagctgac gcagctcatc aaggacgaaa acatcaccgt cgatctgccc accgccttgg
    12361 accgggatca tcaggccaaa ctcgatgatc tgaagggtct ttcggccgat gctttcgacc
    12421 gggcgtatat cccgacgcag gtctccgctc atgaaaaggc ggtcgacctg ttccagggct
    12481 atgcggatgg cggcgagaac gccgatctga agcagtgggc gcaaaagaca ctgccggcgc
    12541 tcaaaggtca ccttgacgag gcgaagcagc tctcttccga catcaagatg gcggccaatg
    12601 acaaagacgg gcaggccacg accaaggatc ggcaggccga ggtcaccgtc gagacgaata
    12661 aggacgacga tgtccgtgtc gaagcgcggc agcggaagga tcgcgcggcc tcgttcgagt
    12721 atgtgtcccg ccagaagccg accgattgga ccgctcaggc cctgatcggc agaacggtcg
    12781 agaatgacgc cggcgacaat ctgggcgaca tcaacaacgt catcctcaat gaaaagggcg
    12841 acgtggttgc cgtcaccatc ggagtcggcg gttttctcgg tattggcgag aaaaatgtcg
    12901 gcgtgccttt ccaggcgctc gagttcagga cggacgaggc acgggcggat cgggccactg
    12961 agagaaacga cgctgacgag aagaaggagt cagccgataa gcgtcgcgaa gctcgcttcg
    13021 acaccgagca cagcaatatc cgcatcgtgc tggacgcgac caaggagcag ctcgaagccg
    13081 ctcccgagtt cgtgtggctg gacgagcaga gcactggcag cgcgcgcgag gaacgggccg
    13141 ttcaatagcg aagaactgcg cgttcaatag cgggcggctt tcgcaggctg ggtggcaacg
    13201 ctgcccagtc tgcgaattct tgcgccggcc tcgatcctgc tggggtgaaa gtgcggctgg
    13261 ttgtaaacaa ccgccgctca tgctcgcttc agacagtcaa gattggttcc ctaattgcgc
    13321 aaaaaagttt ctgggcggaa ctctatcccc gctagatcgt tgagttttca gcaatggaac
    13381 acgattaggt aggagatgtg attatgcgag gaacacttgt tgcagccctc ttcgccggtt
    13441 tggcggctat ggcctttgcc acgccagcgt tggcgttgcc atcttcgacg agcattcagc
    13501 tcggcgcacc caacccgggc gtcatccacg tcgggcggag agaccgctgg cggcactacg
    13561 atcggcgata ccgcgggtat tcgtactacc ggccgtatta caggccgtac ccctactact
    13621 actctcggcc gtacgcttat tacggctacg gcggatatcc gtactggtat gggcgacctg
    13681 gcatcagctt cggattttcg ttctgaggct ggctgatttc ggagaggggc gccctcgctc
    13741 ggcgcccctt ttcaactcgg tgagcggctt tcgcgccccc gagatggata gcaataagga
    13801 gggataacag gccgcggggg cggtcacgaa ggtgatggag accgcagatg ggctccttga
    13861 aacggcggca gacgggctcc ttgaaaaaac cgcctgcgcg ctcgcaaaaa caacagtcca
    13921 cgttcgattc gaacgagctt gagatcatcg atcgcgttta tcacgcagcg tgggcgaaga
    13981 ttctcacccg ccatcccgga catgacatcg ataaggctga acagcgccag acggcgcttc
    14041 gcaaacggtt gttcattctg gctgcccggg ggccggtcga attcgacacg ctccgcgacc
    14101 gcgtgctggt cacaatgcca gagactttga tgaaccccac gcgccgatag gaacccgcca
    14161 tgtctaggca aactggctca tatgtccgtg ccctgaagct gacagcgttc ttgtccgcgt
    14221 tggtaggcgc catccttctc caagctgtcg agcccgccac ggctgctctt aagcacgtta
    14281 ttatcatcga ggccgttgac gtcggctttc cggcgctcat ccatgaggag acgccggagc
    14341 acatggagat tgcgcccgcg aatacgatca gcgtcgagaa ggagcccgcg gccgcccgtt
    14401 gacatccccg cgcggctagc ccagaccctc ctctttcagc agagcgcgat tgatcgccac
    14461 tcccgccttg gctccttcgg caatggccac agccacgagc aggacttctc gggaggcgtc
    14521 tccggccacg taaacgccct tcacgctgct ttcttctgtg acgggatcgg tgacgatgcc
    14581 gcccttctcg tcgcgggcgc agccgagcct ggtcgaaaga tcggagccct ggcggcaccc
    14641 ggttgtaaag aacatggcgg tgcgggccac ggcttcgccg ttctgcaaat ggatcctgcg
    14701 caaggctccg tcgcccgtac cctcaagcct gctgatcggc tcggtccgca cctcgatcgc
    14761 ctgctgccca agccgctcca agaggtccgc cgagatgggt gacgcgccgc cggtgaacaa
    14821 catcacgtcg cgagaccact gcttcatcat cagggccaag ccggcgccct tgtcgccggt
    14881 gccatagacg gcgatagcct ggtcccggtg ctcgaacccg tcgcaatatg gacagtggtg
    14941 gacgctccgg ccatagaggg tgtcaatgcc gtcaatttgg ggaatctcgt ccgtcacgcc
    15001 cgtcgcgagc aagaccttgc gcacattcac ggtgtcgcca ctggcggcgg caaccaggaa
    15061 gcggccgtcc aatggggtta tctcggttac cgggtcgggg acgcccgtca cggtcggata
    15121 tcgtgcaagt tcggatctgc ctttagccaa caactcagcc ggcggcgtgc cctcttgtcc
    15181 gagcaggcag tgcacggcgc gcgacgcgcg atttcgcggt cgtcctgcgt cgaagagaga
    15241 cacgctccgc cggcagcgcc cgagcacgag ggctgcgctg agaccggcag cccccgcccc
    15301 tacgatggca acatcttgtg tttcgttcct agcttccctc tagccattga caccgagctg
    15361 cactaatgag tcccaccggc ccagacctac ggttcgcagg gtatacggaa cccttgcggc
    15421 gtgcggctgg ttgaaatccg gccgagagcg cggttggcgc aaaccgaggc gttcgaggca
    15481 tgcgcgccac gcgggcatcc cgacccgccg acaccgtcga gacaagcagg tttggaatgc
    15541 gcctcctcat tctcgtaaac ccgggggcgt cgcgggccga cgccgcgctc gaggagctgg
    15601 ccgcctggtt ccagcagaat tgcgacgcga cctttgtgac cacgcgctca cgtgatcatc
    15661 tcacgcagat cctgctgcgt cacggccatg acgtcgatcg catcgtgatc ggtggcggcg
    15721 acggcacgat atcggaagct ctgccgtgct tgcttgacct cggcaagccc ttggccgtgc
    15781 tcccccttgg aaccgccaac gactttgcca ggtcgctcgg cctgccgcgg gactcgtttg
    15841 ctgcggcgcg catcgccctc gacgggccga cgcacaaggt agatgtcggg ctcgtcaatg
    15901 gcaggccctt catcaacgtt gccagcgtgg ggcttgcagc acaggtcagc caggtccaat
    15961 cgaaggaatt gaagcgaatc tggcggctct tttcctatgc gatcggtctc ttgcgcgcgg
    16021 tgatccactc gcgccccttc cgcgtcgacc tgacgatcga tcagcactgg acctggaccg
    16081 gcttggcgta tcaggtcagc gttgcgaatg gacgttacca cggcggcggg ctgagagtgg
    16141 cggaacatgc cgccatcgac gacggaaagt tgaacgtcta tttcgttctg ccggggaagg
    16201 tctggcaact cgtcgcggcc atcatccacc tgaaattcgg tttgacgaag ccggagatcc
    16261 tgcagcagaa gagcggccgc cacgtcacga tccgcacccg gaggccgcgc ccggtcaacg
    16321 ccgacggcga gatcggcacg gcaacgcccg cggacatcac gctgcggccg aaggccctca
    16381 gcgtgatcgt gccgccgctc ggcggcgggg aggagtcgac cctcgtcacc cagaccgggc
    16441 aagcacgtcc cgttgataga gacgctccgc ggtgatccct gccgagtcag ccggagcgac
    16501 cgcgcttttc cctgcgtaac cgcagcttct tgccggctag gcgttcgagg taggccgact
    16561 ccaaccagta gatgccgacg agagtggcca acgcgaagat cacagcgatg gcgatcacgt
    16621 tccaggcggc cagcccacag gctatgccga gcgccgccgt aagccacaca gtcgaagcgg
    16681 tcgtcaatcc ttcgacggtg tgagccttat ggtcacgcag gatcacaccg gcgccgatga
    16741 agccgatgcc gcccatgacg ccttggatga tcccctgcac cacgcggctg agagcgtcgg
    16801 gatggtcatc gagattctgg aagtggatcg ttgcaactga gacgaccgcc gccccaagcg
    16861 agacgagcgc aagcgtccgc atgcccatgg gcttgcccgc aagatcgcga ttgagcccga
    16921 cgagcacgcc cacaaggacg gctgaggcca aacggagcgc ggcgtctgcg gtgtcaggtt
    16981 ccatgtgtca tagtaaattc acagaaccat agatggttca ttaacggggc catagagggt
    17041 tctgacgccc gcgtccggcg tccgcgcggc tgcggaacaa cttcatcgcg cagacattct
    17101 tgcttgagag gatggtcgct caaccgatat ccaagaccct tcggccgctc gatgaccaaa
    17161 acgggcctcg gccgcacgcc gcctgagagc ccgaccttag agacgccgtc atgagccccc
    17221 gccccgcgcg accgaacgat tttgacgagt ttgtaaaacg gagcctcttc gttgccgccc
    17281 tcgtgggtct cggcattctg ctctggttct tgtggggcgt gctgctcctg atcttcgccg
    17341 gcgctctctt ggctattgcc atccgcgacc ttgccatgcc gtttgcgcgc tggacgccgc
    17401 ttggaaccgg tggcgcaatt gcccttgtgg tgattctgct cctggtcgtc ttgtcgggca
    17461 ccgggcttgt gcttggaacg cagctcatcg ctcagctcgg cgagttgtgg acgaggctgc
    17521 ccgagttcgc aggaagcgct cgacagttca tcggccaggc gccgatcgga cggacggtgt
    17581 tgcagctgct ccccgattgg gaaacgatat tccgagggat caccgccgga agcgccttga
    17641 cggccgcgac aaccacgctt ggcgtcgtcg ccaacgttct gatcgtcatt ttcctcgctt
    17701 tgttctttgc cctcacgccg agactctaca cgcggggcct ggtcgcgcta ctccctcccg
    17761 ctcatcgggc tcgtgggcag gaagtgctgg cggaaaccgc caaggcgctg cgggcctggc
    17821 tgcgcgggca gttcgtggcg atgatcgccg taggggtcgt tacttggatc ggcctcacgc
    17881 tgctcggcgt tccacttgcc ctcggtctcg cgttcctcgc gttcctgctc gagttcattc
    17941 ccgtgcttgg accgatcgct gcgtctatcc ccgccatctt ggtgggcctg tcgatcagtc
    18001 cactgctcgg gctctgggtg gcgctgctct atatcgtgat ccagcaggtc gagagctacc
    18061 tcctgcttcc gcttatccag cgctgggcgg tcaatcttcc gccggcgctc accctgatcg
    18121 ggatcgtcgc cttcggcgtt ctcttcggtt ggatcggcgt gctgttggcc acgcccttga
    18181 tggtcgcgac catcgtgtgg gtcagggagg tgtacgtcga atcaattctg gagcccgaga
    18241 agaaacagta acggtcccat tccgcgagga tggaacgatt gcctccgcaa tttgttgtcc
    18301 ggccagcaac gcaaactcgg aggttcacca tgaccaaagc aactgcccac ccggatcatc
    18361 gcctcatttc aagcgaggac gttcacggca ccaacgtgta cggcgttggt gacgaagcta
    18421 tcggggagat cgaccacctg ctcatcgaga aggtttccgg tcgcgtctct tacgccgtca
    18481 tgagtttcgg aggatttctc ggcctcggac acagtcacta tccgatcccg tggtctgccc
    18541 tgaagtatga caccgcactc ggtggctatc gcacgggcat cacggaagga cagctgaggg
    18601 atgcgcccga gtttagtgac gactcctggg aggatcgcga ctgggagact cgcacgcatg
    18661 agcattatgg cgccccgccc tattgggggg atggccgcag agaagcagcg tagccttgcg
    18721 ctgtgaggcg caagcctacg cggaagcccg gccccaagag gaccgggctt ctttcatttc
    18781 aggccatgat ggcgccgggc cacttggcac ctgccaaccg gttgcaccgg caatggcttc
    18841 tgcgaggcga tgcggcgcca caggcttggc gcacacgtga acctcaggca gcgcttcgcg
    18901 aatgcgggcg acagtgtcgg gatcgctgtg gccggtgacg aacaacaccg ggatgtcaca
    18961 agcgtcccgc agccatttgg cggcctttat cccctgcaaa ccctcgtcga gatagacgtc
    19021 catcaacacg agatcgggcc ggcggctggt ggcaagctcg accgcctctc ttgggttcga
    19081 ggccagtcca caaacgtcga aaccgagcgc gcgaaggatc gcctccaagt cgatggctat
    19141 caggggctca ttctcgacaa tcaatgcgcg ctgggctgcc atcaggcttc ctttctcgaa
    19201 gtgcccttct cctaacgtct aggcgcgctt tttgtgccga tgttttcgcg caaaggcccc
    19261 gctttttcgg cgcaatttct caaggccgct ggggccaata tgccggaact ccgccgcgtt
    19321 cgggacgttc tagagcgcaa gagacgggac aacaggagat tatgaccatg gttgcgatgc
    19381 atgtcttgag cagaacggcc gcagcgctcg ttgccgcgct ccttgccgcc ggcttgatgg
    19441 tgggagccgc actggccgcc gccagcgatt tcgaaggcac gtggaagacc caggatacga
    19501 agggacaagc cttcgagatc atcctgtcgg cggatgggaa agccaagggc agtcgggccg
    19561 atgaaggctt gaacgggacc tggaaagtcg atggcgactc ggccctgatc acttgggact
    19621 cgggctggac gaccaagatc gtcaaggaag gcgacaagtt caagaagctc gcgttcgaaa
    19681 agggtcagag cggcgaccca acgcatacgg cagacgctca gaaaatgtaa cgggcgcttc
    19741 gcgcgcaccg gttgaccaca ccgctctctc cgctcgtttc ctcgccggct gctccgccgg
    19801 cgcggggacg agaggcttgg gcatgctgct tcgatcccga caattgtctg agcgaccgtt
    19861 ttccgagggc atccggtccg gccaatcgcg gggacgccag aacttggaac aggatcccgg
    19921 gtcgcgcgtt cccggccctg taaggagaag acgatgaaca cgcacgtcac tgacgaagac
    19981 gtttcagttg gtccacccaa gcccaaggac cgaagggttc tgatgacgct gatcggaacc
    20041 gtggccgtga tcgttcttgc gctgatagca ttcgtcgcct tgagcgactt ccctggcccg
    20101 tccgaaggca gcgcagacaa ggcgacggcg cctgcggagg acgtcaggcg ctaggaacat
    20161 ctgataaggt cgcgagttgg agccgcgtgg aggggtttgc aaaggagata tcaatggccc
    20221 gccacatcgt aatggacccc tccggtcact cgaccttcga gttcaatccg ggcagcctgg
    20281 ccgatcttgc caaggccgaa cgccgcttca acaagctttt gaacaaggga tacgtgcccg
    20341 cctatcaacg cggcgaaggc acgcatcgtg ttccgccgca agagaatagg tctttcgatc
    20401 cgtcggccga ggagaccatc ttcattccgg cgcttcgggg cggctaatga acatggtttg
    20461 gctgatggtt gctattgtcg ttcttagcgg cctcgccatg ctcgtttgtt ccatccgcca
    20521 gcgcatggca gagacaaaag gcctgacgct tcttgtgagc aatctcagtg aggagcagcg
    20581 gcgtcagtac gaagcctttg gctatttcga cgcgatcgga tccatcacgg gcaagcgcta
    20641 ccggatccat cacggcaatt cgcggaacgt cgtccagctg ggctccggca acttgcgttc
    20701 cggccgatgc tttgcgccgc gaggcgatct cgttccggga gattgcatgc ttgcgcaaaa
    20761 gatcgcgatt gagaattacg aagcggaagt gctcgccaaa gcgctacctt tttgaggcgc
    20821 tttccgtcgg gatctcggcc ggcggtcggg ccggcgcgtg gagaggaacc gtgatcgcgt
    20881 cagccgcgtt cttttgtcat gggtgcggat cggaagacaa agcgacggcc gcaacgagcc
    20941 cgcgggaagg ccgccaccgg cgaagcggct caaacgcacg ggaccaaatc cgccaagcgc
    21001 gtattggcgg gcgcgccgcc gccgcaaggt ttcccggcga cagcgcccgg agcgccatcc
    21061 cccgatgaaa gagcgccagg cacgcgcgaa cgtggatcgc cggaccagaa ggggatcgat
    21121 gaccgggaag ccagcagccg agaggcaaaa agctagcgca gagagatttc catctaccac
    21181 gcttggattt gtcaggccgc cagacgagcg gttagaaatg cctgccgaag cgagaggctg
    21241 cgcctggcag cggcccgacg cggcaacacg ggcgtcatag ggttctcgcg gcggcagcgt
    21301 gcaaatcacg acggcaaacg gcaagcatga cggtcgatca aggtttggcc ttcgccatcg
    21361 ttaccatcat gatggtcctc ttcgcctggg gccgctggcg ctatgacctc gtggcggcga
    21421 tggcgctgct tgcggccgtg accgtcggta tcgtgccgcc ggatcaagcg ttcaaaggtt
    21481 tcagcgacga cattgtcatc atcgtgggga gcgcgctcgt ggtgagcgcg gcggtcgcgc
    21541 gatcgggcgt gatcgagcgc gtcgcccgct cgctcggtcc ccgtctcgtc acgattcacc
    21601 gtcagatcat cgcgctggtc ggctcggtga ccctgctttc cgccttcgtg aagaacgtcg
    21661 gggcgctcgc catgaccatg ccgatcgcct tccagctcgc gcgccgcagc gggacctctc
    21721 cttcttcact gttgatgccc atgtcgttcg gggcgcttct cggcgggacg atgacgctcg
    21781 tcggcacatc gccaaacatc atcgtgtcgc gcatgaggga acagcttctc ggctcgccgt
    21841 tcggcatgtt cgatttcacg cccgtcggcg ccggcattgt cctggccggc tttctcttcc
    21901 tcgtattcgg ctggcgactc ctcccccgcc accgcaaggg cgccgccgcg atagacgccg
    21961 cgttcaatct cgaggcctat acgaccgagg tgagagtgcc cgaggagtcg cccgccgccg
    22021 gcgtgacggt gaaagcgctt gaggatcttg gcgagaacga gatcgaggtc attacccttc
    22081 tcagaggcgg gacgcgacgg tacgagccgg cgggcaacgc gctgatcaaa gccggcgaca
    22141 ttctcatttt gcggggggag ccgtcggcgc tcgaacgcgt catcgttctg gagaagctga
    22201 agctgacgcg cgaaggcaag cgtcacgcca tcgacacgcc cagcgacgag atcggcgtga
    22261 tggaagccgt tatcacaccg gactccgagc tggtgggctc ttccgcgggc cagctgcaat
    22321 tgttcgaccg tcacgcgatc aatttgctag ccgtcagccg caagggaagg cacatccatc
    22381 gcttgcgcgc ggtgaagctc cgggcaggcg acgtggtcgt gtttggcggc aatatcgctg
    22441 ccatgccgga gacgctcgga gagttgcggt gcctgccgct cgctgggcgc gatctgcgta
    22501 tcggtcgcaa agcaagtttc ctgccgctcg ccgttcttgc cgccgccatg gccctcgtcg
    22561 ccgtcaatgt cctgccggtg gccatcgcct tctttggcgc tgccataacc ctcttgctgg
    22621 tgcggtcgct gtccctgcgc caggcctatg aggccatcga atggccgatc ctcatcatgc
    22681 tcggggcgtt gatcccggtc agcgatgcgc tgcgcacgac cgggggcacc gatctgatcg
    22741 cagcctggct ctcgagcgcg gcgtccgcgc tgccgccgat gggagcgctc gcgctgctac
    22801 tcgtcagcgc catggcggtg acgcccttcc tcaacaacgc cgcaacggtt ctggtgatgg
    22861 cgccgattgc cgcgggcttc gccggcaggc tgggctatag ccctgatccc ttcctcatgg
    22921 cggttgcgat cggcgccgcc tgcgatttcc tcacgccgat cggccatcaa tgcaacacgc
    22981 tggtgatggg accgggcggc tatcgctttg gcgactattg gcggctcggc ctgccgcttt
    23041 cgctgatcgt gatcgccgtg ggagttcccc tcatcgcctt cttctggccg ttttaatttt
    23101 tgcctcgagc cagggaacgt tcaccgggag ggccggttag gcggtcaggt gcccggcctc
    23161 agccacgagc cgggcccgca ccaacaaacc gtaggagcaa gtcaaatgaa gcgtcaaaca
    23221 tcgactttga tcaccgcggc ggcgtttctc gtcgctttcg gtctcaccgc ggcacaggcc
    23281 ggagcccagt ccgacccgat ggcgccaggc ggcggaccgg ccggcagtat cggtgccggc
    23341 gaaggcggag gcatggcacc cgcctctccg ggaggcgggc ccaaccagcc cggcaacgcg
    23401 cccggagcct cggatatgat ggacaccggc ccgggtagcg ccgcctccaa gggctcgacc
    23461 gcggaagcgc cgccatcggc aaaagagcct aagggcgcgg ccggcacgca aaccggcatg
    23521 ccctcagata gaaaggatag caggccggac ggcgacaagg ccggcacgac cgcggaaggc
    23581 caacccgaca ccaaggacgg catgtccaag gacgacaagc aaggcgccac ccccgaaggc
    23641 aaagacgaca aagaaggcat gtccaaggac gataagccag gcgccaccgc cgaaggcaaa
    23701 gacgacaaag acggcatgtc caaggacgac aaaccaggcg ccacggccga aggcaaagac
    23761 gacgccaagt ccaagggcaa gagcgcgcgg cttgagtcga aggacatctc gaaggtgaga
    23821 agccatttca gccagaacaa gccgaacgtg aagcggatcg acagggacga cttctcagtg
    23881 tcgatcggca tcgcgttgcc gggctcgatc gtactgtacg atctgcctcc cgatgtgatc
    23941 gtggtcagcg gcccctgccc gatccaatat ttcgtctggg gcgacgatat cgtgctggtc
    24001 gattcctgct cgcgcgaagt cgtggaaatc attgccggcg tcgcatagtt cccgggagcg
    24061 aaatccacgc gaaagaagac ggccgctccg gcggccgtct ttttctgggc aggacgaccg
    24121 agcctttgtc atgatgccct caggcctctg attccacgtg ccgtgcggcc gttcggacga
    24181 cgggagctca cttagacggg agggctaaac catggctatc aagcatgcga tccggcaagt
    24241 actgacacga ttcggagaac tcgccgcgca tcctctcgcc ttcggcatct ggctgctcta
    24301 cgccgtggtc tggctcattc ttgatcggga ctcgctcgat tggcacgggg tggccacgat
    24361 ggtgacctgg ttcatgacgc tgatcattca gcgcgccgag gatcgggata cccaggcgct
    24421 gcaagcgaag ctcgacgagc ttctgcgcgt catcaaaccg gccgacgacg cactcaccga
    24481 gatcgacacg ctggagccgg aagacatcaa gcgtttgagg aaagagacac actcttcttc
    24541 cggcgagaaa acttaagacg attgacttgc cgcgatcgta accgagcgga ccatccgtgc
    24601 cggaaacacg atccgcgaga tggtgccgcc gccaccctgc atttcgaggc gtccggcaag
    24661 ttgctgagtc agaatctgca caatgttcag cccgaggctc tttgtcgccg cgaggtcgaa
    24721 gccgggcggc agtccgttgc catcatcggc aacctccaga acgagactgt ccccgccgtt
    24781 ctggcggaga tcgatattga tggttccccg tgggcgaccg gcaaggccgt gctccagcga
    24841 attgctgatg agctcggtcg cgatgagcgc gatcgggatc gcctggtctg ccgggagttt
    24901 taccgtcacg gactttacca gacagtcgac gtcgagggca ttccaggatt tcacgatgtc
    24961 atcgcacagc cccttcagga acgcgccaaa atcgatcgcg gcaccttgcg gatcgtagag
    25021 tttgcggtgg agcttcccga tcagctcgag cctgcgggag gcgtcagaca gggccttcgt
    25081 cgcgccccga tcggtgacgc gctccctgtc caaattgagc aacgccgcca cgatctggag
    25141 gttgttcgag attcgatgct gcagttcggc aaacatcgct tcgctttggt ccgccaaggc
    25201 ccgggctttc ccccgttctt cccgcaaccg ctcgatggcg acgttcaatg tatgaacgag
    25261 gagaatgatg agagcgttta tgaagacata aaggacaagc gcgatgacgc tgtcggattc
    25321 gatgttgaag gattggagct gcggcaaaaa gaaataccag gccgcaagca ctgacagagc
    25381 cgtgaccaga acggcaggcc cgacgccggc aaagaaactg gtgaggatga cggcgggaaa
    25441 gaacgtcaga aacggcaatc ccggtggaag caggtcttca aggccgtagc gaacggcgaa
    25501 cgcaacgata aacgcgatga tggcgaagcc ataaccccag ttgcggccgc gacgccagcg
    25561 ttcgaaagtt tcaaggatca tcgatcgcgc acgctacaac gagagcgccc ttttcgggac
    25621 cgtgacaaca ctttttgccg gtgcgaagga acactcgaca acgagccggg tttcccaatc
    25681 aagtaacatt cggggaagtt cttgttcaag aggtgcatcg tgaacatggt ctcgtccgca
    25741 cccgttatcc ttgttgtcga agacgagtac atcatacgtg ccgcccttgt cgccgaactg
    25801 gagcaagcag gttggaaggt tctcgaagca cgcacgggcg aagcggcgct tgctcagctc
    25861 cgtggcgaac agcggatcga cgttcttgtc acggatattc aactcgccgg atacctgagt
    25921 ggttgggagg tcgccgaggg ctttcgggcc gcaacgcccg acatacccgt cgtctacatg
    25981 tcgggcaaag ccgtcgataa atcgcgcatg gttccaggca gcgtttttct gagcaagccc
    26041 ttcgcgccgc aggacgtctt ggcggccgcg cgcgagacca tgcaaatgag ccaacggaca
    26101 gcctaggcga agatgtgcgt ttcgactttc ggcgagagaa cgcaacgtcc tacgcggctc
    26161 tgaggttcaa ggaatcggcg acgaagttga ttgcgatgcg cgtgccggtt ccgggaacag
    26221 acgtaacgtc aagctcggtt ttggcgtttt cgcgcagggt ttgcagaatc agcgcgccaa
    26281 gcttttcgtc cgccggccag gtgacgccgg gaggaaggcc gatcccgtca tcggcgacga
    26341 tgacgcggca gccatcaggt tcttcacgca ggcattcgag cgtaatggtt ccactgtcac
    26401 ggcccacaaa tgcatatttg aaggcgttgg tgaggagctc attgacggta aggccaatcg
    26461 gcatggcgac gttgatcgaa accggcacga tttccacctt gacgtcgagc ctgatcccct
    26521 cgaccgcgtg cgtgcgcatg acaccggcgg caatctgact cagatactgt ccgaggtcga
    26581 tgtcttgccc ggccggcgtc gccgacagcg cgtgataaag aaactggagg gattcgatgc
    26641 ggccggccag acgcttgaaa gcggtctcct ctccgttgcg ctgggaccgc gcttcgagcc
    26701 tgatgagcgc ggtgatgagc tggaggttgt tcttcacccg atgctggagc tctttgagca
    26761 agagatcctt gtcgcggatc tgacgcgcaa attcttcccg ctgactgcgt tcgcgctccg
    26821 tcacgtcgac aagggcggcg atgcggtatt tctccgtgcc ttcgtcgtct tggatcacgc
    26881 cggcatagac ctcgacgaga gcgggtttcg gctcctgcaa ctggaatgtg ccgaccgaat
    26941 cctcatctgt cagaagcgca tggccgagcg tgagctgggg atcgtcctcg ttgcggaacg
    27001 cttccaggat cgaccagctg cggcctttta ttttcccaac gtcgtggcct gtaagggctg
    27061 caaacgcctt attggcgtaa acgatccgct gctcgccacg cacggatttt gacacgatca
    27121 cggcgatggg aacgtgatcg aggaagtgct tgaactcttc aaagccgacg gtgcccgcga
    27181 tctcgaccgc gtcgaagatg tcgttgactt gcgacgccgc gtcggaggct tgttcagtca
    27241 tttcggaatg ctggatcgcc attggctctt cttggaccgg cttggacggg taggttcggg
    27301 gttgaaccgg gggccgctgc cacggccttt gcaacaatag ccttgctgtg accagattat
    27361 ggcgcaatcc cctgcggtgt gaagggccta aaccgggaag gcggcagggg gcctgacctc
    27421 ggcggctccg cgtgcctccg gccggcccga agtcgctcca cgcggccgac cgaggacttt
    27481 gtcatgacag ccggcggctc cagtgggccc cagccaacga gcttgcgagg tgacgttgga
    27541 gaggcggtgc gtcgggctcg gaggcggggg cccggacggg gggtcaagcg gctatctcga
    27601 cctttttgag gttgagctcg acacgtgctc gggcttgccc ttgcgtttgg tcgaggccat
    27661 ctcatcgagc tctttttcgc tcattgagtc gtacatgctt ctcgatgcgc ccttgagcgc
    27721 gctgaccttc tgtttgccgc gttttgcggc cagcgctgcg cccgccgcct tctgctgagc
    27781 ccgagatttc gctggcatga tgtctctcca caataagggc ctcgagaccc cccgcggccg
    27841 tctcctgagg cggagcacga aagccgtatg aacgcgtccg aagcgatttt gatccggctc
    27901 acggcgagcg gataagccct gaccaagatc ggtagtgttt ggaacaactt gtctctcgtc
    27961 gcgttgacgg ggcctaacga cagcagcggt aaccgcgagc ggtttaccgc aagcgtttac
    28021 cgcaagcggt tcgccgtgag cgtttgccgc ggccatacag tcgagaagaa gcccggggtc
    28081 tcctgcaccc tcccacctgc cccgggcttc ttttgccagg cggcttcgat gcatgctttc
    28141 gtgaagaggt ttggaacgca acgctgcgag ccgcgtttcc gatacgaacc gtagtcaccg
    28201 tgatccaagg aaaggaatcg aacccatgtc ctctaccgac acccccgata tgcgccccag
    28261 cgcggccaag cggccaccct cagccgcggg atcggcaagc cgtgatgcga gcgacctctc
    28321 cgatcagatc gacgcgatcc gaacggatct ccagagtttg agctccaccg tcgggcgtat
    28381 cgccaatcgg cagctgagcc gcgcccaaga caaggtttcc gagaccgcgc aggacgcgga
    28441 ggacgcgatc cgccgcaatc cgctctcggc ggtggccatt gccgtcggac tggggttcct
    28501 gttcggcgtg ttcacgcgcc gctagcgaca agcgtccatg cttcgcaccg cgctcgccct
    28561 gctttcatcc ttacaggtcg gtgcccgcat caaggagacg tacgaccgtt gggtccggca
    28621 agggatcgcc gtggcaatcg ccgccgtcct acttttggcg gccgcggcgt ttggactctt
    28681 ttctgcttac caggccctcg tcgcccccta cgggttcact cggcttgagg ccgcggcgat
    28741 catggccggc gtgctgctcg cgttcggact ccttgtcctc gcggtcgttc ccgccttggg
    28801 gaagaagcct cgggtcaggc agccggtcat gctcaccagg tccggcgaag gcatggacct
    28861 cgtggaccgc ggcgtcggac gggccatgca acaggtgggc cccctcaccg tgctcgtcat
    28921 cgcttttgcg gcgggacttt tggcggggcg ccgctgaacg gtttgcacgt tctcgtccgt
    28981 ggtcttgtgg ttttgcgggc tctctcgatg cgggcaccgt ggcgctgtca cggatcgcgg
    29041 tgggggcatc gtcttcgccc ggctcctcac tcttgagttc tcccttcgcg gcggcgatgg
    29101 cgatttccgc ggcctcttgc agggacgcgg cgtaggcgtc ggcttcggct tccgccagca
    29161 cttctttgac ggcgttcgca tccccctccc cgccgaagaa tgccacgagg atcgccgttc
    29221 cctccggcag gatgcggcgc agccggcgga caaggtagcg ggtatgagca gggccggcgc
    29281 cgaggccgag atagcatagg cagacaaatt tcgcttcggt gccggcgagc gaggcgatat
    29341 ggcctgccga gatcgtttcc gtctcaagcg ccgccgcgcc gagcccgcgc ttcttcaaca
    29401 cctcggcgag caaggcggca gcagcttcgt caagagccgt gcgtccgcca atgcagagaa
    29461 cgggctcgtc cacctgccat ccgggcgcaa gctcggcacg gtcgatctcg ggcgccgcct
    29521 ccgtctcatc tccttcattc tcggcagcgt cgagcgaggc gagaccggtc ggggtctcgt
    29581 cgggttcctc cgctttttgg cgcagcttgg agaaccagcg gcgcggttgg aaatcggcaa
    29641 gatcgtcgag catctccttg accgtatcgg aaatgcgctc cgtctgctcc gcatcgagaa
    29701 ggccgcggcg cgcgtcccgc tcggcaagct tgaggcctgc gagcgccacg ttctcgaaac
    29761 aggctacgaa accctgatcc ttgaggcaga gttcggcctg gtaggtggcc tcggccgcat
    29821 ccccggagag cgcgcgctga tagaagctct gatcgggagt cagcgccggc tcatcgccaa
    29881 gcagtacctc gaacacatgc aaatcctcga cattgcgacc gagcacggcg agacagaccg
    29941 tgatcggcat ggcaaggagc agcccaagcg gtccccacag ccaggtccag aacacggtcg
    30001 agacgacaac cgcgatcggc gacacgcccg tgccatgccc gaacacgaga ggctctacca
    30061 cttggcccat cgtcacttcc gaaacgacgt agagcgcaag cacgatcagg aaggtcgacc
    30121 aacccggatc gaccaccgcc gcgagcaaga gcggcggcat cgccgcgata taaccgccaa
    30181 taaagggcac gaaccgcatg agcatggcga gcacccccca tacgaggggg gttggaatgc
    30241 cgatcaccca cagcgcaacg gttatgaagg taccgaaggc ggcattgatc aaaagctgcc
    30301 gcaggaagaa cttgctcaag cgggtcgccg cgtcgttcag cgtcgtggtg gcgcgctgca
    30361 agtcggagga gcccagcaac ctgatgaagc ggtcgcgcag atcctcgcgc tggagcagga
    30421 tgaacaccac gaagaggagg acgatgcccg cggtcgcgag cggcggcagc aacgtgccgg
    30481 cgatttgccg gaagatttcg acggaggtcg gctgaggctg gcggacctcg accggcatgg
    30541 gacggtcggc cggtcgctcg gcctcggcac cgacttccgg tgtcggcggg gactccctcc
    30601 cggacttgtt cagctccttt tgcaggtcgg aaaggacgtc accggccttc ttcagtacgg
    30661 aggaactcgg cgctccctcg cgcaaacctt tgatcttttc cgtgagcgcg tggcggtagc
    30721 tcggcaagtc ggcggcaagc tgcgtgatct cgcgcgacat gaaccagccg agggcgaaga
    30781 tgatggcgaa cgcgcagctc accacgatgc cgacggcgat gacgcgcggc aagccgatcc
    30841 gcctgagcag gacgagcggc cgggtgagaa cgaaactcaa gacgatggca accgtgagcg
    30901 gaaggagcac ctcgcgcccg aaatagaggg cggcgatgac cagcgttgcg atgagcaggc
    30961 cttgcagaat cgcggtgcgg gcgtccgagg gagcggagag gacggggaaa cgcgatcgag
    31021 gcggcatgtc ggttctgtcc gcacgcaagg ttgtcagctg taagaggccg gcccttttgg
    31081 ttcctggctc aggcccccga cagtatcaga actgaaggca gcgcaacggc aagtcgcgaa
    31141 tggataacgg aaaggggatt ccgacgagcc gcaagagaac gccgcgcatc atggtttcct
    31201 cgaccggatg gctgttgtct tgcgcctagc aacgcgcgat gtgtcttgag gttccaagcc
    31261 ggccgcgacg ccgtcccctg tggcggcttc ggcttgaccc ggctcgcccg ctcgcgattt
    31321 cgttcaggaa ccgcccggct tcgcggctgc cgggtcgcgc gtcacggggc tcaccggccg
    31381 cttttcgccg ccgggtcccc cggccacgtg atcggcgacg aaggcgcccc gcttgcccat
    31441 cggacgcggc tcgcccgtgg tcgtgtcctc cgccgtctgc agctcgagct cggcattgat
    31501 ctccgcgccg agcaagacga tgtagaacga catgtaaagc cagaacaaca atatgacgac
    31561 ggcgccgagc gacccgtaga ccctgtcgta gttggcgaag tgcgacacgt agagagagaa
    31621 gcccgccgaa gcaatcagcc acgccaccat ggcgaacaga gagccgatgc tcacccaatg
    31681 ccatttggcg ctgcggcggc aagggccata acggtagaga agggacaaga tgaagaggac
    31741 gaagagcgcg aggaacggcc atcgtcccac gccgacgacc agctccagcg cgcgcgaata
    31801 gcccgcgacg gcaaacagaa tcggcacgta gacgatggcg agaagcatga ccacgccccc
    31861 gaccatgccg atgatcgtga aggtgagcgc gttcaaatag tattggaaca ggctcctgcg
    31921 ctcgggttct tcataggcga tattcagggc tgccatcatg gcctgcgtgc cggcaagggc
    31981 gctccacagg gcaaggccca ggctgacggc aagaccccag ccgagccttc ccccagatgc
    32041 cgcggcgacg cgttggatct gatccatcac catgtcgctg gcctgcggag gaagcacgga
    32101 ccgaagaatc ccgaaatgct gctccacagt gtccgggctc gccgttaggc catagacgga
    32161 gatcaccgcc gacagcacgg ggaaaatggc caggagagca aagaaggcgc agccggccgc
    32221 cacgacggag agattgtcgc ggttgctctc ggtccagacg cgctcagaga cctgccacaa
    32281 gcctttgagc ggaatgtggt agggaagtgt tgcgtcgcgg ccgtaaggat cgggcttgcg
    32341 gttcatgaat gaccgtgtcc gccgaggccg ggaatggcga tgacgcgcca gaacccgctc
    32401 ggccgacacg agcatactcg tctccccgag gcccgcctag gagtctagag ccgcccaagc
    32461 agaagcagga tgagcacgac gacgagaatg acgccaatga tgccggaagg cccgtatccc
    32521 cacgcccggc tatggcccca ggtgggaagg actccgatca aaatcaggat caatatgata
    32581 acgaggatgg tcgtaaggtc cataagactt cgtcctccac cgcatagttc ggccggcatc
    32641 gcgcgcggcg gcaaagcagt cgttttcgcc gagagacgcg agccttggct gaacttaatc
    32701 gattcgcggc ggaattgttc ctggagagac cttgcgaacg gccgaaaact ccgactttcc
    32761 cctggaaatt gcggctgccg gccctccgcc gacgggatgg gctcccaccc gccgagaatt
    32821 ggccccgcct gcaagacttg cgcgttatat gcgatcgttg ggcgaatatg cccctgtcat
    32881 acccctcatc atcggcgaag atggcacgaa aaacggaagc gcaacgcagg aaggaagcca
    32941 aggagcgagc ggaacgcgcc ctccgcaaaa ccaaggagag actgggcctc ggtcagcccg
    33001 gaccgctccg aggcgcagtc cccagtccgc ttgctgcgat cagcaagcga cgccatgcga
    33061 aattgccttc cacgtccgat cgcatcccag gatcagctcc ggcttcggac gtgctccatg
    33121 ggcataagtg gaagcgaggg gtcgaggaaa gcgacgcgac catgcgcgag atgcagcgaa
    33181 agtctatgcg aattgcgccg gcttacaata agggcgccct gcaatatctg cccaagggcg
    33241 tcgatcgcga cgaataaccg ggaagatcac cccagcgatg cgcgtgagcc tccgaaaccg
    33301 cgccgcaaac tacgccgcta gcggtggaac gcgatccggg agccgtgccc gcccatggaa
    33361 gtcctgatga agattccgct ttcgggcctg atcaacgtcc gaagcccgct tcggagtttc
    33421 ggcaaaggcg tttgaggtcg gcttcggcct cttcctctgt cggataggcc tccttgccga
    33481 ttctggtgac accctcgtca cccggggttg gctcgatatc caccaccagg cacttctcgt
    33541 cggaggagcg cacgatccac caatttgcaa ggctcggagt gggagcaagg atcccgatca
    33601 gaacagcagc atcgatcaag agaacgcgaa ggaaccgcgt acgtgtcgga tcgccactcc
    33661 cggatgaatc agtcatcgat ttttcgacct ccatgcacga cgtttggcgg cgcgcgttcg
    33721 gcgaatgatg cgaccgattt tcgcctgact gccgcaacca cgtccccgcg gggatgttcc
    33781 cagctcaacc acattttttt aagcatggaa ccgccgtctc ctgacatcgg tgccagctca
    33841 tttctgccct tggaaaaggg cgccgcaggt tcggtgagga tgcgaaagag caagaagcat
    33901 gatggttgcg ctccatccgt gaatagggct tgaacaacgg taccccgtcc ggcgcttctg
    33961 cagtgctttt ggcggaaaca acttggtttc cgcggcgttt tcataatgcc gcttcaaaac
    34021 ctcgttcgga gccaacacca tgcagatcgt taaccgcctt ggcgcagctc tcgcctttgt
    34081 gacctcggtt gcccataggg gccgcccgca gcgacgatgt cgtggctctg ccgtgggcag
    34141 acgccaaggt cgaaagcctc gcaactcttg ataatccgtg ggcgatggcg tttctccctg
    34201 atggccggct gctgatcacc gagaagcccg gcaatctgcg cgtctatgcc gacggcaagc
    34261 tttcaaagcc gatcggcggc gtgccgaagg tggaatatca cgagcagggc ggcctgctcg
    34321 gcacggccgt cgatccgaac ttcgccgaca attagctcgt ctatctgtcc tataccgagg
    34381 cggcgccgga gcagcctgcc gatgccaagg acgagggcga tccacgtctc ggcgaatatc
    34441 aggatctcaa ggatgtggtg ctgaagggcc ttgccgttgc ccgcgggcgg ctcgacggcg
    34501 atcaactcaa agacgtgcag gtcatttggc ggcaaacgcc caagacgatt ggacgcgggc
    34561 attttggggg acaactggta ttcgcccccg acggcacgct attcattacg tccggcgatc
    34621 gccagagatt tgacccggcc caggatctcg gctcgacact cggaaagatc gtgcggataa
    34681 acccggacgg ttcgatacct caagacaatc ccttcgcgaa ggagcccggc gcgctgcccg
    34741 aaatatggag cctcggtcac cgcaacccgc tcggcgcggc attcgagccc gggtcgggca
    34801 agctgtggca agaactacgg ctggccggtc gtgagcgagg gcagccacta caacgatgtg
    34861 ccgattccgc atcatgcgca gaaggccgag ttcatgccgc cactcaaatt ttggaatccc
    34921 tcggtttccc cgtcaggcct cttcttttac accggtgata cgttcaaggc gctgaagggc
    34981 aacgccttgc tcggcggctt gtcgtcggag gcattgatcg tgctcaccgt caaggacggc
    35041 aagccgctcg atgacaacat cgcaagggtc gggcaccgcg ttcgcgacgt tgccgagatg
    35101 ccggacggga gcttgctcct gctgactgat ggcgacgacg gcgagctcct tcacctcaca
    35161 ctctcgcgtg agaaggaagc cggttagagc ggagcacggt ggggcacgcg atagttcgat
    35221 cgccggaacg catgaagcgg caactcgttc cttcgtcagg cagcgtaaac cttccccagc
    35281 tggttaggcc cggtgccgtg tcattcctcc cttgtggcgg cagcggtgcc gggccgctcc
    35341 gcctccgagc cgcgatgaaa cttcacccca gcttgcgtgt tccttcttcg ccggacgttc
    35401 gaagcctgtc cgcaaatcaa accttctaaa agccaggagt gggctcgtga gcgattggac
    35461 cttggatgac attgcggaaa agctgcgcga tatcgacttt gcgatgttgc tgacgcagag
    35521 cgaaagcggc gagattgcgg cgcggccgat gagcaacaac cgcgaggtgg aatatgacgg
    35581 cgattcgtat ttcttcaccc tggaagagac ggacatggtc gcgcatatcg agcgcaattc
    35641 gaaagtcgcg ctctcctacg cgggaaacaa gggcctgctc ggcaaaccgc cgatgttcat
    35701 cgccatgcga ggcgtggccg agctgatacg cgacaaggat cggttcgcgg cgcattggag
    35761 cgaggatctc gagcgctggt tcgaggacgg tgtcgataca cccggcctcg tgctgatcaa
    35821 ggtccacgcc gaccgcatcc actattggga cggtgaggac gacggagagc tcgaggtctg
    35881 atcgcacctt cgctcttgac gcttgtcgtt ttgcgattgc ggagctgcgg aactcccgct
    35941 gtcgagcgtc gttgtgtcgg tagcagtcca aattggaaag gaagccctca tgggaaacaa
    36001 gcaacggcaa aagagcgaca atccacctca gaagcacccc gaacaggtgg agcaaaatcc
    36061 cgagcaccga gaccgggact ataagcggga ggagacgaat atcgatcgca gcgagcgtcg
    36121 cccgggtcag caaacagaaa aacaacggcg aacgggcgaa cgcgacgccg tcgagggcga
    36181 aggcgaggcc gaggacgaga gcgccgtctc ctagagcatt atcccatcga attgaatcat
    36241 gaccccctct tactcccccc gggagcgtgg ttcaatgcga cgaaataatg ctctgcggat
    36301 cgatcaatcc ccaggactgg accttggcga aatcgccgtc gatcagtttc tgcccgatgt
    36361 cacgccgata ccgggcatta ggaatcacca gcttgccggc caattcgtgc gccgcgtccc
    36421 gagccgccgt gatggtgtct ccggtgcccg tcaccacgag cgtgtaaccg tttgcgccgc
    36481 tggtcacgag gacgccgtcg ctcaggccca cttccccgta atggaggttg gcacgctcgg
    36541 cagtcgaaag ctcgcctttg aaactgatcg gcaatccctt cggctctaag acatgctcgc
    36601 gtgaataggg aaacggaggg gttgtcatca cgatgccgac gcagaacccc ggttcgacct
    36661 cgaacctggt ggcgctgcgg tccagcatcg cgcgaaacaa tgccccccat cccgttcgct
    36721 gcaacggatc gaggatggcg tagccgggat aaccgaagcg gcaagtgaac tcgagcggcc
    36781 agatgccttg cgcgttgacg atggtgttga ggttgatata gccgcaatag ccgttctgct
    36841 cgagaagcgg acgcattttg ccgagcgtgc gctcgaagaa cgtcttgccg ccgctgtagg
    36901 tcacgaccgt ccccatttca ccggtgagtt cgcccatgtc gtgcggaaaa aaccgtttat
    36961 gctcccagtc gagacaggca ggcatgagga actcgacacc gttgaaatag gcgccgacgc
    37021 ccatctcgat gccttcgacg aactccatca aaacaaaact ggcggttgcc aaattcggat
    37081 cggaggaaag cacggaccgc acatccgctc cgtcgggccg gctaccgaca aacgttgccg
    37141 cgtcgggccc gtttgatttc acgacatagc gcgcgggccg ttcggcaatg aaccggcgcg
    37201 cctcttcgat ctgcgaaaac gtgaatgtcg cagccgtcgg caggccgaga tcggcaagaa
    37261 cgttctgcgc gtaagtgcga tcgttctcca ggcgcgcgcc ataggcgctg ctgccgatga
    37321 cattgaaacc gtcggctctc agacggtcct ggagctctcc ccgacccgag ccgacattct
    37381 cgaacaagag gcagccctcg gatccggcct cgcgcaccca cccgagctcg ttctgccaat
    37441 cttccacgtg cggtacgaga ccgagaaggg tatcgcggca aagaggattg ccgatgtaga
    37501 tcttgacctc gtgtccctcg gcggcgaggc gaagataaag cgatccgaga tcggcagcgt
    37561 caccgatccc cagaaaacgc atccaccgat ctcccgaggc taagcggctt ggctctgccg
    37621 cacatcgagg ccgaagtgag cacgatagcg ctcaaagcaa cgggcgccgt gccacaatcg
    37681 gataaccgca ccttcgccga tcgccgcggc gcaacgaggg tcctcggcaa cgagcgaacg
    37741 cagcacctct cggttccagc actcggagtg cttgatgtcc aagacggcat gcaaggtgaa
    37801 atagaggcgt ttcttcgtcg gcacacgcag tcgcttcagc ccggcatcga caaagccggc
    37861 ccgcgttggc gccgtcatct cgatcgcgcc gagcgcgccg atcgattgaa aacagtatcg
    37921 ccggctacgg gccatcgcca acatggcgtt gcccaatgcc aaggattcgg gcaccactct
    37981 ttcgggccgc gcatcgatat cgagatagct ggcgagcctt tcgaggagcg gaccgtgcat
    38041 ggctgtgtcc cggccgcggc ccatctcgtc ccaatagttg cgcgccatct ccagcttggc
    38101 ggtgacgggc atcttcacct gcgtgagcgc cagcaggtcg tcgaatcccg cctcgccggc
    38161 aacttcctgc gtcaaaaacc agagcatttc atcgcgggtc gcggcttcgg ccagccaggg
    38221 aaatagggga tcgccctgcc ccggacccgt ctccttgagg tcctcgaacc agtcgatgaa
    38281 atcatcgaca tcgcttggga cgtcggccaa tagggggctg atctcgtcac gtaccgcttc
    38341 gatatattcg ccctcggcac gcaggacctc agcgtatgtc ttcagctcgt cacgccacgc
    38401 ctggtgaggg aaacggggtc ccagctgctg cccgttatat ttgctgagtt cggtctggaa
    38461 ccgttcgaag ccggggccgg acaactcgcc ggcgtctttc gtcgaaaagt aatacgtcac
    38521 ggaactagct ctcactttct tgctacgagg ctcacaacag cgatgcgatc gacgccggca
    38581 taggagggct tttcgagctc ctcgccaaag acgtcgggat cgacttcctc ataacgaaac
    38641 tgagagcaat agagtttcag gaacggctcg acagaatcga aaaaagggtc acctccggcg
    38701 ataatcgggg ttccggtgta gagaataaga cgaccgcccg cagctaaccg ggccaagcct
    38761 tccgctgcta ttcgcgcggc gaccgcgacc cccaaatccc cgccgccatg ccgatagagg
    38821 cgccgttctt catccaccag ataaggaggg ttggcgatga tgaggtcggc ttcgccttca
    38881 agtccgtcca gaatgtcgct gtgcaacacg cgcgcatgag caacgtcgtt gatggcagcg
    38941 ttgacggcgc tgaagaccaa tgccttgcgg ttgatgtcgg caagaaggat ttcagcctcg
    39001 cccggcaggc agcgcgccgc atagagccct ccagctccac tgccgcttcc gacatcgacg
    39061 agcctcaggc gtctgtccga ggccacatcg gcaaccgagt ctcggacgag tctcacaaac
    39121 cgataggtgt caggcccgaa aaacacggcg tcgggctcga tcgtggggaa ggccgagtgg
    39181 gcgaagacca tgtcgccgat cgtggcaaac ctcacccgcg atcgatagcg accgttgtca
    39241 ggtgccaagg cgtcggcctc cgacaagagc tcgaacatgg ctgttgcgat ttcatcgcgc
    39301 tgaaacgggc ggttccaccc gaatattgac cgtaacgtcg ttgacgcttc aggccgattg
    39361 aggacgaggc cgtgtgtggc cggcgttacc gctacaaatc ggtatccctc ttctttcagc
    39421 gaacggccca acgcgacgag ggcgagacac ttggcgtcca tacccggatg gcctgtagac
    39481 gatgcaccga tggccgagtg cagctagtat cgcgaggccg tacgccaggc cgcggtctcg
    39541 cccgccggaa cggcaaagag atttctcagt gcttcgacat ccaccggctt ctgcaaaacc
    39601 ggggttgtcc ggtaccgggg attgaggctc tccaagccgt aaccgctcac gaaaacgaag
    39661 ggtatgcccc gagcggcgag gtcgtcggcc aaaggataga ccaactcctc tccgagcctg
    39721 atgtcgagaa tggcggcagc gatgggctgt cgcgccgccg ccgacatcgc atcgcccaaa
    39781 ttggtacaag ggccaacgac tttgaacccc agagtcgtga gggcctcggc catcatcatt
    39841 gcgacgatgg actcatcttc cacgagcatc accgcgctgc ccttgtcgat ttcgagggac
    39901 gatttcgccg gcatgttcga tagcttctga ccgccgcccc ggttccggtg ttcggccatc
    39961 tcgccgagcg gcaacaacaa ctgacagagc aggccgtcgc gtcgccagtt aaagtcagcc
    40021 cggccgccaa gctgttcgag actcgccttg atgacacgcg tgccatagcc cttcaacttg
    40081 ggcggcacca cttcaggacc gccctgctcc aaccactcaa tcgtcaagcc gttcgcgcca
    40141 atcccccacg acacactcaa cttgccggcg gcattgctga gagcgccgta tttggccgca
    40201 ttcgtcgcga gctcatgcac ccccaacgcg accgtttgcg cacgtttggc atcgaggagc
    40261 acggcgggac cgtcgtcatt gatggtcacg cgatcgcccg cgcgataggg gtccaactcc
    40321 tcgaccagca tacgcttgag atccgcgccc tcccatcggc tctccgacaa gagcatgtgc
    40381 gcgcgggaga gggctctgat ccggccctcg acgaccgcgg cgtaggcttc ggcggactct
    40441 gccttggtca ggcgcagcat cgactgaacg atggccagca cgttccgcgc cctgtgatcc
    40501 acttccttgg ccagaaacgc ctgacgctcc tccgcctcct tgcgatccgt gatgtcgatg
    40561 gtgacgccgc tgacccgcac cagacgaccc gcctcgtcgg cgctcggcgc ggcggttcca
    40621 aaacaccatc gccgctcacc gtccggccgc cggacgcgga actccgtctg ataggctttg
    40681 gcgccgtcgg aaaagtcgga gatcatttcc tgcaggcgtt ccgcatcctg cggctcgacg
    40741 agcgacatca ccctttcaag ggtcggcttg aatgtctgcg gatcgacgcc aaaaatgcgg
    40801 cattgaccct catcccacat ccagtctccg ttgatgaggt cccaatccca ggagcccatg
    40861 gcaccggccg caagcgcaag atttcgtcgc tgctcgctct cggccagctt gtgcacgtat
    40921 gattcaagtt ccgccgttcg ctcggagacg cggatctcca gttcccggtt caaagcctcc
    40981 aattgccgcg ttttgcgata gagctcggcg aagatcttga ctttggcgcg caagaccgcc
    41041 ggcaccaccg gcaccgggac atagtcgact gcgcccatct cgtagccgcg caggcgatcg
    41101 acgtcggata tatttatggc cgagatgaaa ataattgccg tcttctgata gcgggggtgg
    41161 tcgcggatca tggccgcaag ttcgaatcca tcgaggtcgg gcatgcagac atcgacgaga
    41221 acaacggcaa tctcgttctg aagcagctga ctgagtgctt cagtcccgga cgatgccttc
    41281 aggagatttt cgccgagatc ctggaggatg acctcatagc tgagcagctt tgatggctgg
    41341 tcgtcgacca ggagaatgtt gagtttctcc accgccgtca ttgcttgcac gtcccttatc
    41401 gatgcagcca gctccgcatg gcggagagca gttgctccgt gttcaccggc ttggcgatgt
    41461 attccgaggc cccggcctca agcgatttct cgcggtcgcc cttcatcgcc ttcgcggtca
    41521 gcgcgatgat cggcaagcgg cgaaaggcgg gatcctcccg gatcgccgca attgtttcat
    41581 aaccatccat ttccggcatc atgatgtcca tgagcacgat cgcaaggtca ggcgtggatt
    41641 tgaccagttg tatcgcctca cggccattga ccgcggtgag gacccgcatg ccgcgccgct
    41701 caagcatgct gctgagagcg aagatgttgc gtgcgtcgtc atcgataagc agcgcggtct
    41761 tgcccttcag atgttcgtcc gagcgatgca gccgctcgat catctgctgc ttgtccggcg
    41821 gcaatttcgt caccaatcga tgcaggaaaa gggcagtctc gtccaagagg cgctcgggcg
    41881 actcgacgcc cttgacgaca atgctgcgcg ccatcgtgtg cagttgggcg tcctcctcgg
    41941 cggacaattc tcgtccggta aagacgatga ccgggatatc cgagagagag tcgtccgcgc
    42001 gcaattcctt cagcaggtcg aagccggaca tgtcgggcag acggagatcg agcacgacgc
    42061 aatcgaagcg ctcctcgtta aggagatcga gagcttcggc gccggtcccg gtcgtgacca
    42121 cttcgatgtc gtcgtgtcca agaagctcgg cgatgctcat ctgctcggct tcattgtcct
    42181 caatcaccag gaggcgtttc ttgtggggtt cggcgaaatc cttgatgcgg gagagagcgc
    42241 tttcgagact ctccgtcgag gccggcttgg taaggaaaga gtaggcgccg cgggtcagtc
    42301 cgtgctggcg gtcctcttcc atggtcagga tctgaaccgg gatgtgccgc gtgatcgggt
    42361 tctgcttcag ctggctgaga acggtccagc cgagcatgtc ggggagaaag acgtcgagcg
    42421 agatcgcggt cggcctgaat tccagcgcaa gctcgagtgc ctccctgccc cgcatggcaa
    42481 tgacaacctt gaatccgttc tcgcgcgcga gatcgctgag cacgcgcgca tagtgaggat
    42541 cgtcctcgat gatcagcagg acggcgtcgc ccggctcaat gtcatccctg tcgtcgggaa
    42601 tgcgttcgac cgagtgctcg tagacgaccg tcggcatgag gggccgagcc atctgcggtc
    42661 cgccgacacc ggccggcgcc ggccgcgccg gcaccaccgg tccggcgtag accatgggca
    42721 ggtaaagcgt gaaggtgctg cccatgccgg gcgtgctctt ggagccgatc tcgccgccga
    42781 gcaggttgga aagctcgcgg ctgattgcga gaccaagacc cgtgccgccg tattttcggc
    42841 tcgtgctcgc atcggcctgc tggaacgctt cgaagatgat cttttgctta tcgagcggga
    42901 ttccaatacc ggagtcggag acttcgaacg cgacaacgct ggacacctga ctgaggatcg
    42961 ggtggtcggc gctccatccc tcggtgaccg gatatacctt cagccagacg ccgccctgtt
    43021 cggtgaactt gaccgcattc gacagcaggt tcttgagcac ctgctgcagg cgtttggagt
    43081 cggtcgtgat gctgtgcccg agattgggat caagctcgac attgaaggaa agatcgcggg
    43141 cttccgcctc gtggcggaac gggcgtgcga ccgcctcgac caggctgctg aaggaaatct
    43201 gctgggcgtc gacgctgacg gttcccgatt cgatcttcga cagatcgaga atgtcgctga
    43261 tcagattcag caagtctgtg ccggcggcat aaatggtgcg ggcgaactcg acttgcctcg
    43321 gcgcgaggtt cccttccggg ttctcactca gttgctgacc gagaatgagg atgctgttga
    43381 gcggtgtcct gagctcatgc gacatgttcg ccaggaattc cgacttgtac ttggacgtga
    43441 gcgcgagttc gcccgctttt tcctcgagcg cgcgccgtgc ctgctcaatc tcctggttct
    43501 tgcgctcaac ctccacattg cgctcggcga gctgttgcgc tttttgccca agttgatcgt
    43561 tggtctgttg cagctcacgc tgctgggttt gcagttcggt cgccagctgt tgcgactgtt
    43621 tgagcaaaga ttccgtctgc atcgtggctt caatgctgtt cagcacgatg ccgatgccgc
    43681 cggtcaattg ctcgaggaag gtgacttcga ggtcggtgaa ctcctccacg gatgccaatt
    43741 cgatcacggc cttgacctgg ttctcgaaca ggatcggcaa gacgatcacg tttctcggcg
    43801 ccgccttgaa cagcgccgag ccgatagtga cggcgttgtc agacatctgc gaaatgagga
    43861 tgctgcgcgc atcggcggcg cactgcccga tcaggccttt gccaaaccgg agcaggggcg
    43921 gatgaccatt gacgtcgtcg tcagcatagg acgagagcag tttcaggctc ggctcatcgc
    43981 cgctgccgtc gacttggtag atcacgccaa gctgcgcatt gaccagcggc gccaactcgg
    44041 aaagcaggag gcgtcccacc gtcgcgaggt ctctctggcc ctgcagcatg ttggtgaact
    44101 tggcgagatt ggtcttgagc cagtcctgct ccgtgttgcg gtccgtcgtc agccgcagat
    44161 tgacgatcat cgtattgatg ttgtccttga gctcggccac ctcgccgcgc gcatcgacct
    44221 gaatggatcg ggtcagattg ccttgcgtca cggcggtcgc cacttcggcg atggcgcgga
    44281 cctgattggt cagattggcg gcaagaagat tgacgtttcc ggtcaagtct ttccacgttc
    44341 cggccgtgcc gggcacgttg gcctgcccgc caagacgccc ctcgacgccg acctcacgcg
    44401 cgacggtggt cacttggtcg gcgaaaatcg caagcgtatc catcatattg ttgatggttt
    44461 cggcgagcgc cgccacctcg cctttcgatt tgacggtcag cttctgcttc aaatcgccgt
    44521 tggcgacggc cgtcaccacc ttgacgatgc cgcgcacctg ctcggtgaga ttggcggcca
    44581 tcacgttcac ggtgtcggtc aggtctttcc acgttccggc gacgccgggc acctgagcct
    44641 gcccgccgag cttgccttcg gtgccgacct cgcgcgcgac gcgggtcact tccgaagcaa
    44701 acgcgttgag ctggtccacc atcgtattga tggtgtcctt tagctcgaga atctcgcctt
    44761 tcacgtccac ggtgatcttg cgggaaaggt cgccgcgcgc cacggccgtc gtcacctcgg
    44821 cgatgtttcg cacttgcgtg gtcaggttgg ccgcaaggag gttgacgttg tcggtcaggt
    44881 ccttccaggt cccggccacg ccgggcacgt tggcctgtcc gccaagccgc ccttcggtgc
    44941 cgacctcgcg cgccacgcgc gtcacctcgc cggcaaacga gcgcagctga tccaccatcg
    45001 tgttcagagt ctctttcagc agaaggattt cgccacgcac gtccacggtg atcttcttgg
    45061 aaagatcgcc gcttgcgatc gcggtcgcca cctcggcgat gttacgcacc tgcgccgtga
    45121 ggttgcccgc catgaaattc acgttgtcgg tcaagtccgc ccaagtgccg gcaacctcgg
    45181 gcacctgcgc ctgcccgccg agtttgcctt ccgtgccgac ttcgcgcgcg acgcgcgtca
    45241 cctcggaagc aaacgcattg agctgatcca ccatcgtgtt tatggtgttc ttgagctcaa
    45301 gaatctcgcc cttcacgtcc accgtgatct tgcgcgagag gtcgccgcgc gcgaccgccg
    45361 tcgtcacctc ggcgatattg cgaacttgcg aggtcaaatt gcccgccatc gagttcacgc
    45421 tgtcggtcag gtcctcccac gtgccggcca cgcccggcac attcgcctgg ccgccaagcc
    45481 ggccttccgt gcccacctcg cgcgccacgc gcgtcacctc ggcggcaaac gcattcagct
    45541 ggtcgaccat cgtattgatg gtctctttca gctggagaat ctcgccgcgc acgtccaccg
    45601 tgatcttgcg cgagaggtcg ccgttggcaa tcgcggtcgc cacctcggcg atattgcgca
    45661 cctggccggt gaggttgccg gccattgagt tcacgctgtc ggtgagatct ttccacgtgc
    45721 cggcgacgcc cggcacttcg gcctggccgc cgagcttgcc gtcggttccc acctcgcgtg
    45781 ccacgcgcgt cacctcggcc gcgaacgcat tcagctgatc gaccatcgta ttgatggtct
    45841 ctttcagctg tagaatctcg ccgctcacgt cgacggtgat cttcttggag agatcgcctt
    45901 cggccacggc ggtcgccacc tcggcgatat tgcgcacctg tgccgtgagg ttgctggcca
    45961 tggagttcac gctgtcggtc agatccttcc acgttccggc aacgccccgc accatggcct
    46021 gaccgccgag cttgccttcg gtgccgacct cgcgcgccac gcgcgtgacc tcgccggcga
    46081 aggcattgag ctgatccacc atcgtgttga tggtgtcctt gagctcgaga agctcgccgc
    46141 gcacgtccac cgtgatctgc tttgagaggt cgccgcttgc gatggccgtc gccacctcgg
    46201 cgatgttgcg cacctgggcc gtgaggttgc tggccatgga gttcacgctg tcggtcaaat
    46261 ccttccaggt gccggcgacg ccgagcacat tcgcctggcc gccgagccgc ccttccgtgc
    46321 cgacctcgcg cgccacgcgc gtgacctcgc cggcgaaggc attgagctga tcgaccatcg
    46381 tgttgatggt ctccttcagc tgcaaaatct cgccgctgac gttcacggtg atcttcttgg
    46441 agaggtcgcc gccggcgatg gcggtcgcga cttcggcaat gttgcggacc tgcgccgtga
    46501 ggttgctggc catgaagttg acgttgtccg tcaaatcttt ccacgtgccc gcgacacccg
    46561 gcacagccgc ctggccgccg agcttgcctt cggtgccgac ctcgcgcgcc acgcgcgtca
    46621 cttcggaggc aaaggcattc agctgatcca ccatcgtgtt gatggtgttc ttgagctcga
    46681 gaatctcgcc acgcacgtcg accgtgatct tgcgggagag gtctccgcgc gcgaccgccg
    46741 tcgtgacttg ggcgatgctg cgcacttgcg cggtcagatt gccgcacatg gcgttgaccg
    46801 agtcggtgag gtccttccaa gtcccggcca cgccgggcac gatggcctga ccgccgagct
    46861 ttccgtcggt gccgacctcg cgcgccacgc gcgtcacctc cgaggcgaag gaccgcaact
    46921 gatcgaccat ggtgttgatg gcctctttca gctgcagaat ctctccgcgc acgtccacgg
    46981 tgatcttctt cgacagatcg ccgttggcga cggcgatcgt cacgtcggcg atgttgcgca
    47041 cctgagcggt caggttgctt gccatcgagt tcacgcgctc ggtgaggtcc ttccacacgc
    47101 ccgtgacctc cggcacgtcg gcctggccgc cgagcttgcc ttcggtgccg acctcgcgcg
    47161 ccacgcgcgt cacctcggac gtaaacaggc cgagctgctt gatcatggtg ttgacgatcg
    47221 tggcagagtg caggaattcg ccccgcagcg gccggccgtc gacatcgagc cgcatggtct
    47281 ggaggagatc gccttgggcg acggcggcga tggcgcgggt cacttcggtg gtcggccaaa
    47341 gcagatcgtc gatcagcgtg ttgatcgacg tctccatctc accccaggcg ccgtgcgaga
    47401 cgccgagttt cacgcgcttg cgcgtttcgc cttcgcggcc gacgacctga cccacgcgct
    47461 cgagctgatt ggcgaggcgc tgattggcgc cgacgatttc gttgaacgca tcggcgatct
    47521 ttccttcaat tccggaccgg tcggagggca tgcgcacggc gaaatcgccg acgcgcatgg
    47581 cctgaagggc ctccagcaac tcatgaagga tcgcgccgga cttgccgttt aaagggggct
    47641 cagccgcgag cggcggggcc gccttctcgt ccgggccgtt gaggtcaact gactcgctgg
    47701 tcggctgcat gacgtcgctc ccgccgaagc agattgagat ggttcaggga tgcacagagg
    47761 aaggtgttga agccgccgaa cgtgtcgcaa ggcgctgctt caacattcaa aactggcaca
    47821 attgatgaaa cccgcgggtg ttcgcgaccc tcccacccca gcttcattat gtgagctccc
    47881 gtgaaacgcc cgtggtccag acgagaaacg ccttctgggc caaatggttc ctgccgaatt
    47941 ttattgttta acttaagacc gaggccaatt gggtctaggt cggcaagcac gatcttgatg
    48001 ccgccgcctg ggggtttcaa tacaaattta ccaaccggtc gacggccggc aggcggcagg
    48061 cccctagcgc gatcgggtgg gtccaatgcc ggtgagaagc cactcgacgg agacccggca
    48121 ggcgaggcag aacggcacga tcaaattgtg cggcatgagc gaggcgccgc ggccttcata
    48181 tttggtgtag gtctcccgcg tgacccccag caatagggcc atctcttcca ggggatagcg
    48241 ggccctcatc cgtgcctgcc tgacgcgcgc cgcaaacgca gcgcgatatt ccttcggcgt
    48301 tacgcggcgc cgttttgggg ctgtgattgc agctccgctg cgggttctaa ccattccaag
    48361 tcgtccataa aggccagcga agaaatacca agagacggcc gctcttgtcc tgcaaataag
    48421 gggttaacgg ccgcgcggcc gcagcaatgc tccgtccccc agcacctgcg ctcaagctaa
    48481 ggctgcgagg gcctaacgcg cgtcacggac aagcaacgac atgatttcgt agggaacatc
    48541 ccgctatacg ggaagttgtc tccgcacctc gacattcgaa caaggttggc cgtcgacata
    48601 cgaggacgcg gattcgcgct ccggagcccg cgtgaaacct gaatcctgcc cccttcccct
    48661 tggcgcatga agaacgatgc tttttatcgc ccttgcggtt gactatgacg gtacgctcgc
    48721 ccacgacgga cgggtggacg acgccgcgat ggcggccctg gaaaaagtca agaaatccgg
    48781 acgcaagctc atcctcgtca cgggcagaga gatcgccgac ctgcaacgcg tcttcagcgg
    48841 gatcgagatt ttcgatctcg tcgtcgccga gaatggcgcg cttctcttcg acccggcgca
    48901 aaagaaagag gagccgctct cgcctccgcc gtctgatcgt ttcgtggcgc tgttgcgaag
    48961 ccggaaagtc gcgccgctct ccgtcgggcg ttcgatcgtg gcgacctggg aaccgaatga
    49021 aacgatcgtg ctcgaagcga tccgcgacct cggcctcgag ctgcatatca tcttcaacaa
    49081 gggcgcggtc atggttcttc cggcggatat caacaaggcg tccggcctca aagaggcact
    49141 caagcggctc tcgttgtctc gctataatgt cgtcggcatc ggcgatgccg agaacgacca
    49201 ggcgttcttg agcgcttgcg gctgcgcggt cgcggtggcg aacgccttgc catcggtaaa
    49261 ggaaaaggcg gacttcgttg tcgccgacca cggcgccggg gtggccgaac tcgccgaaat
    49321 cctgactggg acggatctcg ccggccacaa taccggcctc ggcaagattc agcctgtcat
    49381 cggcgaggat gcgggcggga agctgcgcca actcaagcca ttggatacgg ttctcgtcac
    49441 gggatcctcg ggcggcggga agtcgaccgt cgtcacggcg ctgatggagc agatgatcga
    49501 cgccaacctt cagttctgcg tggttgatcc tgaaggcgac tatgcggagt tcccggccgt
    49561 ggcggtgggt gatgcgaaac acgagcctcg catccccgag gtcatggacc tcctggccaa
    49621 gccggatacg agcgtggtgg tcaatcttct cgccatcgat cccggagaga ggcctcgcta
    49681 ccttgccggc ttgttgccgg agctgtcgaa acttcgcatc gaaaccggcc gcccgcattg
    49741 gatcgtgctt gacgaggtgc atcattgcct gccggccaac tgggacccgg cgccggtgtc
    49801 gctgccgcaa gagctcccgg ccgcgatcgc tgtcaccgtc catccggaag aggttgcagc
    49861 cgagttcctc aagatggtga cgacggtggt gggcgtcggc gagcaggcga agaagatgat
    49921 cgagactttc tgcggcgcgc gcggcgaagc ttgcggcacc ttcccggaac ggccggatgc
    49981 aaagcacggc cttgtgtgga cggcgaccgg cggggtgcag gccgtcgccc taaggcggcc
    50041 gaaggtacgt cagaaacggc atattcgcaa atacgcgcaa ggcgagcttg gcgaggacag
    50101 aagcttctac tttcgcggcc cgcaaggcgc gctgaacctg cgcgcccaga acttggccat
    50161 ttttctccag ctggctgaag gggttgacga ccaaacctgg atgcaccatt tgcgcgccgg
    50221 cgaatattcg caatggttcg accaagccat caaggatgag gagttggcgg ccgaagctcg
    50281 tatcgtggag caggatgaaa cgctttcggt cacggaaacg cgcacgcaga tcaagaagtt
    50341 ggtcgagaac aaatatacgg cgccggccaa gagctcgtag ttcgacgttg aagcgttatt
    50401 ttgtggccgg tgatcgcgcg aaacgaacgc ccgtgtgtag gaatttgata tcgggtggaa
    50461 cagagtggtt cttctggcgt tatagatgta cggagtgggc tttgaaggga aaattgccat
    50521 gattaagaca ataggcggtg tcccggtgaa gacgcgcgta ccattagttg gatgggcttt
    50581 cgcgggactt ctaacggtcc tctcgatgac atcttcgctt gtgcagaata tttcgcgtcc
    50641 ggcggacgtt tcagcttcgg cgtttgggcc cttgacaacg tcggagcgag ttgcggcgcc
    50701 aacccgtcac gcgagtgcaa gcgcgagctg gtggtattga gaaaaaccat cgttgacgcc
    50761 gccggcagcg tctcgcgccc gatgggtttc ctgccgaact cagcgtcaag ttcgaagcat
    50821 cttccaagcg tcattttcga tcgttgcgga agctcgtgct tgtctcaaga aagcgcatga
    50881 gcgcgctttc catcctgttg aggccggcgc gcacgcggcc gcggaacaaa gatgctccta
    50941 gcttgccgct ctcataggcc ttgatcacca cgggatggat gtagctcgac cgcgcaacgc
    51001 tgcgcgtgtt gaccaatatc tcgctcgctc tatcggccgc ctgcacgatc gtctgctttt
    51061 tcagcctctc cgtttcgtga ccgttacgtt cggtcaggaa cgccagcgct tcggcggacg
    51121 cccggaaggt gcggaaatcc ttggccgtta cgtcggtgcc gcttgcttcg gcgagaaatg
    51181 cgttgacctc acgcgccgtg accggccgca cccgtccatt gtcgtcaact gcgctgaaga
    51241 gccgccgccc cttgcctgcc agcaggcgcc tcgtgacacg ggcgagcaac ctgtccttga
    51301 cttctctcct gaccggcttg ccggccttgc ccttgaattc caccacgact ttgtcgcctg
    51361 tgacagccac gtcgctcttg agcagggttg aggcgccacg cgagcccgtt gcctgcgcgt
    51421 attcgtcatg gcccggccgc agcagcgcac gatcgatcag cctcacgacg gccgcgataa
    51481 cgttgtttcg ggtcaactcc ggcgctgaca aggccttacg taccgtgtcg cgcagacgcg
    51541 ggagcgccga accgagtcgc cgcaatcgat gcagtttggt ttcagcgcga gcgttctccc
    51601 aatccgcgtg gtagcgatac tgcaagcgcc cttccacgtc gcggcccacg gcctgaatgt
    51661 gagcgcgatc atcctcggcg atgcagactt cggtccaagc cggcggaatc gcgagctgcc
    51721 tgatgcgcgc ttttagcgat ttgtcgcgca cggtacggcc ttcgccgtcg atgtaaccga
    51781 agcctctgcc acagcggcgg cgccgcaaac gaagatctgc cgtctcgacg taggtcaggc
    51841 cataacggct gcaaagagtt ttgacttcgc gtggcatgat caggcgaaac ccgccaagct
    51901 accggtaagt ttcagaaact gggaatttaa tctgccgtcg caagccctcg cttaagggtt
    51961 gccggaagca tctccatccc gcggctgcgt cagaatcgcc ggaagacgaa ccgtaagcgt
    52021 ttgcgccatc gcttttttga atacttcttc ttcgactttt ttgccgtggc ggaacgctcc
    52081 tgctgctcca gcgccccggt ttcgtcatcg tccgctagcg cggcctggtc ctcaccatat
    52141 ccgggaacgc tcggttcttc tgtggcggcg atgtgctctt catagatcca tccctcgcgc
    52201 gaggtcgcgg gatcgcgaat tctgacccag ccaaggtttc gctcgagaag ccgcatttcc
    52261 gcaccggccg tggcgtagcc gagcctggat gacgacaccg acggaccgct atgcaccggc
    52321 gccccgcgca ggaggcgacc ccaggcagga gtcgcttgtt gttcggtgcc taacgaggat
    52381 ggagcttgag tcgaggctgt cccattcggc gcggagtctt gcaggtcttg ctggctttcg
    52441 ggggcgtatc cttgaccggc atcgcctgcg ccttcctcga caacggatag accaccggtc
    52501 tcagtggatg gaccggccgg gtctatcgcg tgcaggttcg attgaggccg cgaacttcgc
    52561 tgcgcctgct cgggtccgtt caatggcgag tttttggccg gtgccggggc ttcgacgggg
    52621 gatggggtga aggcccccgc gacatagaga agagcgccac aaaacgccaa aaatagaacg
    52681 acccacttca tggtcagcac aacgcccgcc tagcaccccc gttcccaagc cgcgggcgtt
    52741 cagcgacgga tgcaacccgt ccccatgaag gaatcccggc atggcagccc ttcttgcccc
    52801 taccgtcatc gctgcgcccc gggccgaagt ccccgggcgg cccgccaact attgctggcc
    52861 agtggccggc gctggcgccg caggcgggct cgccttctcg ccctcaggcg cggtcgcttc
    52921 gtgaacctcc tggcgtacct cttcgccgac cttctcgaac gaggcgccaa gtgacgcgcc
    52981 gacatagtcc caattgacga tgtcccgctt gtcggagtcg ggaccgacat ggtcgacaag
    53041 aaagacgacg agtatggtga gggcgattcc cgtgagcacg cctgaaaaat aaggcatgtt
    53101 ggacctccac aattcccgcc tcagcaaaac gccttgcgac gcgcatagtt ccgggacgac
    53161 gcgtcgacgg cagggcgaca atccgtcgat cgccttagga gagcgcttcg cgtttgcagc
    53221 gcaagggcac gcgacggatc agctcgcctt ctttttcgga accttcttgc ccttcttgcg
    53281 cgcttcggaa agcccgatgg caatggcttg cttgcgactc ttgaccgtgc cgcctttgcc
    53341 gcccttaccg ctcttcaact tcccacgctt aaatttgcgc atttcgcgct caacgtcctt
    53401 agaagcgctg cgtgaatact tcctcgccat gccgatcctc cacgcacgtg cgaacgcgtt
    53461 acgacttacg gggcagatcc cctgacgaaa tgcgtcacca gcgagatcaa caggagcaca
    53521 aggaacacga agaacaagat cttggcgatt ccggccgatg ctcccgcgat gccgccaaat
    53581 cccaaggcac ccgccacgag tgcaatgatc aagaaaacca aggcccaata caacatggtc
    53641 aacctccact tccagtcgag cggagttcag tccgccaaaa gctgaccgtt aaaacacatg
    53701 caacccgccc aagttccgaa gccctaccgc attttccgtg aagcggttca cgcggcttaa
    53761 ccctgccggg ttttcacggc cagttgcgag ggtcgcatgc cttcaagccg gccggcctgg
    53821 ccaccgacat cgacgggcac gacagagacg ggccggttcg cgtgcgcggc atttgaccgt
    53881 tgggcgctgg aactgtgcgg tcacggcttc gttcccctca tataaaggag aacgccatgg
    53941 cttccatgca ggactgcggg caaatatgca tcaaggcggc ccgacggttc acgccttcgt
    54001 aaagccctgc catgaacaag caatcgcatt tggatcgggt ttgggaggtt gttggcaagg
    54061 tcggcgtgtg catgatgacc acgcactttg cggacggctt gcgcgcgcga cccctcgaag
    54121 cgagagcgga ccgagacgaa aacgtcattt ggtttttgag cgatcgccgg ggagccaaag
    54181 acgacgagat cgaagcctat ccttcgatct gcctgacctt catcgatcca aaggaaaagg
    54241 tttacctgtc gatttccggg acggcatcgg ctgcgcgcga tccagagcgt gcgaagaatc
    54301 tctggaacaa agaacagcaa gcgtggtggc caaaaggccc gagtgacccc aatgttctgg
    54361 tcatcgcgtt caggccgcaa agagccgaga tttgggacgg gccggcgagt tcagccgtcg
    54421 cgtcatatga atttgcgaaa gcgcgcgcga caggcacaaa gccgaatctc ggcgagaacc
    54481 gcaaaagcac ggtgaaaatg gactgagagc ttcgacattc gccgcatggt gaggcacgcg
    54541 gcgacgatcg gggcgcctgc gattagcgag gctcgacgtt cgggtccgct cccggggtgc
    54601 ccggcggagg aatgacttcc tcgtcatgac cggcctcggg gtttgggacg tcgtccgtat
    54661 agatttcctc atcgcctatc gggggaggcg tgatgacgcc gttgcgctct atcacggcgg
    54721 agccgcgtgg cgttcatcgc acgttcgtta atgccgatgc aatctcgcgc atgatgctcg
    54781 gacgccaggg tgcggtgcgc ttgtcgccag atgagacgtg accttcgggc ttggaatcac
    54841 cgaggcgtcg aggatgcgct cgcggtgcta ttggccgccg gcgctgttcc cgtttgggcg
    54901 gtcgcctct
//
LOCUS       DASUQP010000107         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_320816,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000107 DASUQP010000000
VERSION     DASUQP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320816"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /gene="acnA"
                     /locus_tag="VFY74_04820"
     CDS             <1..1663
                     /gene="acnA"
                     /locus_tag="VFY74_04820"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX5931059.1"
                     /translation="AYAREQGLFRTTGAPDPEYSDVLELDLATVEPSLAGPKRPQDRV
                     PLAHAAASFAEALDKEYGKAAEANLRVPVKGKNFDLGHGDVVIAAITSCTNTSNPSVM
                     VAAGLLARNAVERGLRVKPWVKTSLAPGSQVVTDYLAEAGLQTDLDALGFNLVGYGCT
                     TCIGNSGPLPDEISHTIHTHHLAVAAVLSGNRNFEGRVNHDVRANYLASPPLVVAYAL
                     AGSVARDLVNDPLGTSADGAPVYLRDIWPAAEDITKVVRKAVKKSMFETRYGDVFRGD
                     PEWRAIDVKGGLTYDWDPHSTYVQNPPYFAGMSLKPSPVTDIEDARILGLFLDSITTD
                     HISPAGGIRRDSPAGQYLIEHGVAVPDFNSYGARRGNHEVMMRGTFANTRIKNQMVPG
                     VEGGVTIHYPSGERMAIYDAAMRYHEEGVPLVVLAGKEYGTGSSRDWAAKGPRLLGVR
                     AVIAESFERIHRSNLIGMGVLPLVFAEGHSWRSLQLKGDERVSLEGLNALKPMQWIEA
                     EIVSANGEKRAVRLRPAIDTFDELDYFRNGGILPYVLRSLAARRA"
     gene            1734..2489
                     /locus_tag="VFY74_04825"
     CDS             1734..2489
                     /locus_tag="VFY74_04825"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX5931060.1"
                     /translation="MTRFIRFSAGAFLTLAAIAIATPMKAEPPAPASKAVLELFTSQG
                     CSSCPPADALLAQLGKNPDLVTLSYAVDYWNYLGWHDTLSSSANSERQRDYARGRGDG
                     KVYTPQLVVDGLTHVNGSNEAAIEMAMRSAAKRLADLRVAVNMHAEGDTLVVGIGAAP
                     EKSDKRAATVWLAIAKKTATVAITRGENRGRTLSYHHPVKELTPVGMWKGEPVTLRLP
                     LKDLKAMGGDCLVALLQVENAGPILGAAEYDLL"
BASE COUNT          516 a          944 c          916 g          517 t
ORIGIN      
        1 ggcgtatgct cgggagcagg ggctgttccg caccaccggg gcgcccgacc cggagtattc
       61 cgacgtgctg gagctggacc tggcgacagt cgagccgagc ctggccgggc cgaagcgtcc
      121 ccaggaccgc gtgccgcttg cccatgcggc ggcctccttc gccgaggcgc tcgacaagga
      181 gtacggcaaa gctgcagaag cgaacttgag ggtgccggtc aagggcaaga atttcgacct
      241 cggccacggc gatgtggtga tcgccgccat cacctcctgc accaacacgt cgaacccgtc
      301 ggtgatggtg gcggcggggc ttttggcgcg gaacgcggtg gagcgcggct tgcgggtcaa
      361 accctgggtc aagacctcgc tcgcgccggg ctcgcaggtg gtaaccgact atcttgcgga
      421 agccgggctc cagaccgacc tcgacgcgct cggcttcaat ctcgtcggtt acggctgcac
      481 cacctgcatc ggcaattccg gtccgctccc cgacgagatc tcgcacacga tccacaccca
      541 tcaccttgcc gtcgccgccg tgctctccgg caaccgcaat ttcgagggcc gcgtcaatca
      601 cgacgtgcgc gccaactatc tcgcctcgcc gcctctggtc gtggcctatg cgctcgcggg
      661 ctcagtggcg cgcgatctcg tcaacgatcc gctcggcacg agcgccgacg gcgcgcccgt
      721 ctatctcagg gatatttggc cggccgccga ggacatcacc aaggtggtgc gcaaggcggt
      781 gaagaaatcc atgttcgaga cgcgctacgg cgacgtgttc cgcggcgatc ccgagtggcg
      841 cgccatcgac gtcaagggcg ggctcaccta cgactgggac ccgcattcga cctacgtgca
      901 gaacccgccc tatttcgccg gcatgtcgct taagccttcg ccggtgaccg acatcgaaga
      961 cgcgcgcatt ctcggcctgt tcctcgactc gatcaccacc gaccatatct cgccggccgg
     1021 agggatcagg cgcgacagcc ccgccggcca atatctcatc gagcacggcg tcgccgttcc
     1081 cgacttcaat tcctacggcg cgcggcgcgg caatcacgaa gtgatgatgc gcggcacctt
     1141 cgccaacacc cgcatcaaga accagatggt gccgggcgtc gagggcggcg tgacgatcca
     1201 ttatccttcg ggcgagcgca tggcgatcta cgatgccgcc atgcgctacc acgaggaggg
     1261 cgtgccgctc gtcgtcctag ccggcaagga gtacggcacc ggttcttcgc gcgactgggc
     1321 ggccaagggt ccgcgactgc ttggcgtccg tgcggtgatc gcggagagct tcgagcgcat
     1381 ccatcgctcg aatctgatcg gaatgggagt gttgccgctc gtctttgccg agggtcactc
     1441 gtggcgttcg cttcagctta agggcgatga gcgggtgagc cttgagggcc tcaacgcgct
     1501 caagccgatg cagtggatcg aggcggagat cgtttctgcg aatggcgaga agcgcgccgt
     1561 tcgcctcagg ccggcgatcg atacgttcga tgaactcgac tatttccgca atggcggaat
     1621 tctcccttac gtgctgcgga gccttgctgc gcggcgggct tagtcactca cttgtgaccg
     1681 cgagcgaggc gttttggccg gaaatttcgg ctcgtttcct ccatgctagc gccatgaccc
     1741 gcttcatccg gttcagcgca ggcgcgtttc tcacgcttgc agccatcgcc atcgccacgc
     1801 cgatgaaagc cgaacctcct gcacccgcga gcaaggcggt gctcgagctc ttcacaagcc
     1861 agggctgctc ctcctgcccg cccgccgatg cgcttcttgc ccagctcgga aagaaccccg
     1921 acctcgtgac gctgtcctac gccgtcgatt attggaacta tctcggctgg cacgacacgc
     1981 tttcgagctc cgccaacagc gaacgtcagc gcgactatgc gcgcgggcgg ggcgacggca
     2041 aggtctatac gccacaactg gtcgttgacg gtcttaccca cgtgaacggc agcaacgagg
     2101 cggccatcga gatggcgatg cgaagcgcgg ccaagcgcct cgcggaccta agggtcgctg
     2161 tcaacatgca cgccgaaggc gacacgctgg tcgtcggcat cggcgcggca ccggaaaaat
     2221 cggataagcg cgcggccacg gtttggcttg cgattgccaa gaagacggcg acggtcgcga
     2281 tcacccgcgg cgaaaatcgc ggacgcacct tgagctacca ccatccggtc aaggaactga
     2341 ccccggtcgg catgtggaag ggcgagcccg tgacgctccg gctgccgctc aaggacctca
     2401 aggccatggg cggcgattgt ctggtcgcgc tgctccaggt cgagaatgcc ggtcccatcc
     2461 ttggcgcagc cgagtacgac ctgctctagc cgcaaaacga aacgggccgg caagccgacc
     2521 cgttcgacgc gttcctcttc gaaagtaatg gcccggtccg agagaagacc ccgggggggc
     2581 tggggggctg agatccgaag cctctccatt ggaccgggcc gagaggcaac gttgagcatc
     2641 atacgtttct ttggggcgtg gcaacggcgc aactggggcg gaactgtgat ttatgtttcg
     2701 ttatgttaca ctttgtgtaa cgcccgccat caacttgaat ttcgctaacg cccggctcat
     2761 catgaagctt ccgttacagg aggcgcaagt ttgagcgaaa cggaacccat agtgttggcg
     2821 ctggccctca aggccggcgg cgctgcaaac tttgccgagg ctggtcttcg acgcggcagt
     2881 atcgcgtttg acc
//
LOCUS       DASUQP010000108         9223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1820312, whole genome shotgun sequence.
ACCESSION   DASUQP010000108 DASUQP010000000
VERSION     DASUQP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820312"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..134
                     /locus_tag="VFY74_04830"
     CDS             15..134
                     /locus_tag="VFY74_04830"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931061.1"
                     /translation="MLGNILSCDGEWCNVSVEDYSGYVQQDRLWGVYRGEEVK"
     gene            complement(146..595)
                     /locus_tag="VFY74_04835"
     CDS             complement(146..595)
                     /locus_tag="VFY74_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5931062.1"
                     /translation="MHRSPSVLSRLPAEPTLGDIARILREAGLRPTRQRVALAQLLFA
                     GDDRHVTAEMLHEEALARKVRVSLATVYNTLHQFTAAGLLREVAVEGAKAYFDTNTSN
                     HYHFFCEHSGKLMDIATGSIRIEGLPEAPEGTAISRVDVLVRLVDKR"
     gene            850..1380
                     /gene="fabA"
                     /locus_tag="VFY74_04840"
     CDS             850..1380
                     /gene="fabA"
                     /locus_tag="VFY74_04840"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEX5931063.1"
                     /translation="MSERRDQFDYEDLLACARGELFGPGNARLPLPPMLMFDRICEVS
                     EDGGEHGRGRIVAELDVKPDLWFFPCHFKGDPVMPGCLGLDALWQLLGFFLGWLGAPG
                     KGRALSVGEVKFSGMVLPTMKKLEYIVELKRVIRRKFTLGTGDGWLKADGETIYTAKD
                     LRVGLFQEGEVAPAGA"
     gene            1426..2640
                     /gene="fabB"
                     /locus_tag="VFY74_04845"
     CDS             1426..2640
                     /gene="fabB"
                     /locus_tag="VFY74_04845"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEX5931064.1"
                     /translation="MKRVVVTGMGIVSSIGNSAQEVLASLREAKSGIVKAETYAELGF
                     RCQVHGAPKLDWEGMIDRKVRRFMGSGAAWNYLAMEQAVRDAGLEEGDISNERTGLIM
                     GSGGPSTRAIVQAADATRAKGAKKVGPFEVPKAMCSTNSATLATPYRIKGVSYSISSA
                     CSTSAHCIGNGAELIQLGKQDVVFAGGGEELDWTLSVLFDAMNAMSSDFNDRPEVASR
                     AYDADRDGFVIAGGAGVIVLEELEHAKARGARIYGEVAGYGATSDGHDMVQPSGEGAV
                     RCMRMAMRNVDGIDYINPHATSTPIGDVKEIEAIREVFGDKCPPISATKSLTGHSLGA
                     AGAHEAIYSLLMMNNGFICESANIETIDPAVADMPIVRKRQDNVSIDSVMSNSFGFGG
                     TNATLVFRRLDG"
     gene            2687..3571
                     /gene="fabI"
                     /locus_tag="VFY74_04850"
     CDS             2687..3571
                     /gene="fabI"
                     /locus_tag="VFY74_04850"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5931065.1"
                     /translation="MTTTRDIAKPAPLMAGKRGLVMGVANDRSIAWGIARVLHGQGAE
                     LAFSYQGGAFGRRAVPLAESIGSKIIVEADVEDLKSVERVFDAIGKTWGSLDFVVHAL
                     AFSDRNELKGRYCDTTRKNFEHTMVISCFSFTEICKLAAGLMPSGGALLTLTYGGSSR
                     VVPCYNVMGVAKAALEASVRYLASDFGPQGIRVNALSAGPMRTLAGAGISDARTLFHF
                     QQRNAPMRRSPTLDEVGGAALYLLSDLSGAVTGEVHFVDCGYSTIAMPNISALKTMEQ
                     AEEGERATEKVPPREAAQ"
     gene            3699..3896
                     /locus_tag="VFY74_04855"
     CDS             3699..3896
                     /locus_tag="VFY74_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931066.1"
                     /translation="MAELIVMVCLLATPEKCQEFTVPGASTGDVVSCIRQGGDKSTAW
                     QTQNSQYFVIGWRCVMNKTAQ"
     gene            complement(3961..4086)
                     /locus_tag="VFY74_04860"
     CDS             complement(3961..4086)
                     /locus_tag="VFY74_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931067.1"
                     /translation="MPLRVSALKRHWNGLPEPVPGLDPGIKPGNDEKGKFLIETG"
     gene            complement(4136..6256)
                     /gene="pnp"
                     /locus_tag="VFY74_04865"
     CDS             complement(4136..6256)
                     /gene="pnp"
                     /locus_tag="VFY74_04865"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5931068.1"
                     /translation="MFKRETVEIEWGNRPLKLETGRIARQADAAVLATYGETSVLATV
                     VAATEERPGIDFFPLTVNYQEKTFAAGKIPGGYFKREGRPTEKETLTSRLIDRPIRPL
                     FASGFRNETQIVATVLSHDMENDPDIVAMVATSAALTLSGVPFLGPIGAARVGYIGGQ
                     YVLNPMIDEMPESALDLVVAGTADAVLMVESEAKELPEEVMLGAVMYGQKHFQPVLDA
                     IVKLAERSAKEPWELKPKDEGELVTRVRELGESELREAYVIAGKHERRDRVAALKDKV
                     VAALVPADDDLTLAAKVASAFKEAEKEIVRSSILNSKTRIDGRDLTTVRPIVCEVGIL
                     PRTHGSALFTRGETQALAVATLGTGEDEQYVDALQGTYKETFMLHYNFPPYSVGEVGR
                     MTSPGRREIGHGKLAWRAIHPVLPDRESFPYTIRIVSEITESNGSSSMATVCASSLAM
                     MDAGIPLKRSVAGIAMGLIKEGEDFAVLSDILGDEDHLGDMDFKVAGTSEGVTSLQMD
                     IKITGITEEIMKVALDQARDGRMHILAEMNKALNTARPELGEYAPRIETIHIPVDKIR
                     EVIGSGGSVIREIVAESGAKIDISDDGTVKIASANAESIRAAINRIKGIAAEPEVGEI
                     YKGKVVKVMEFGAFVNFFGPRDGLVHISQLADGRPKTVNDVVKEGDEVWVKLLGFDDR
                     GKVRLSMKVVDQKTGKEIARAEAG"
     gene            complement(6372..6641)
                     /gene="rpsO"
                     /locus_tag="VFY74_04870"
     CDS             complement(6372..6641)
                     /gene="rpsO"
                     /locus_tag="VFY74_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5931069.1"
                     /translation="MSISAERKQELINEYATKSGDTGSPEVQVAILTERITNLTEHFK
                     THGKDNHSRRGLLKMVSQRRRLLDYVKSADEDRYQKLINRLGIRR"
     gene            complement(6889..7869)
                     /gene="truB"
                     /locus_tag="VFY74_04875"
     CDS             complement(6889..7869)
                     /gene="truB"
                     /locus_tag="VFY74_04875"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5931070.1"
                     /translation="MARQRKGLPVHGWLILDKPAGMTSTEAVSRIKRLYQAAKAGHAG
                     TLDPLATGVLPIALGEATKTVPFVVESCKEYRFTVRFGAETDTDDAEGKVVAQSDLRP
                     SCAEIEAGLADFTGEIQQVPPRYSALKVDGARAYDLARDEEIFALQPRRVSVDWLRLI
                     EHPDKDRALLEARCGKGTYVRALARDLGRAFGCYGHVEALRRTRVGPFAEDRAVALAA
                     LGARAEEGGREALMPALEPVETALEGMPALSVSAQDADRLRRGQAVLLRGRDAPIVEG
                     TVYATSRGTLVALGEVERGELHPRRIFNLPTGSRSPLVGEHARRLARGEG"
     gene            complement(7891..8280)
                     /gene="rbfA"
                     /locus_tag="VFY74_04880"
     CDS             complement(7891..8280)
                     /gene="rbfA"
                     /locus_tag="VFY74_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5931071.1"
                     /translation="MSHRDKAKPPSQRQLKVGEMIRHALAEIFARGAIADDVLNRHSL
                     TVPEVRMSPDLKLATVYVMTLGGGEAEEAVAHLDKHKRYLRGLLAKRISVKFMPELRF
                     KVDTSFEAASRIDELLASPKVARDLDG"
     gene            complement(8480..>9223)
                     /locus_tag="VFY74_04885"
     CDS             complement(8480..>9223)
                     /locus_tag="VFY74_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX5931072.1"
                     /translation="VVENEARAREITDYRVRERRKRLAVAGARGSLEQMMNQLKEAGI
                     AEFPLLVKGDVQGSVEAITTALAKVSTDEVRARLIHAGVGGITESDVSLAATSGAVVL
                     GFNVRANAQAREVAEREGVEIRYYAVIYDLVDDMKQAMSGLLAPTVRETFLGNAEILE
                     VFTISKVGKVAGCRVTEGKVERGAQVRLIRDNVVVHEGKLSTLKRFKDEVREVVAGQE
                     CGMAFEGYQDMRQGDVIECFQVEHVKRTL"
BASE COUNT         1599 a         2937 c         2967 g         1720 t
ORIGIN      
        1 cgcttcaggc gggcgtgctc ggcaacatcc tatcttgcga cggagagtgg tgcaacgtct
       61 cggtcgagga ctattccggc tacgtgcagc aggacaggct gtggggcgtc tatcgcggcg
      121 aagaagtgaa gtaggtcgcg agccgtcagc gcttgtcgac gaggcgcacc agcacatcga
      181 cccgcgagat ggccgtgccc tcgggcgcct cgggcaaccc ttcgatgcgg atcgatccgg
      241 ttgcgatatc catgagcttg ccggaatgct cgcagaagaa atgatagtga ttggatgtgt
      301 tggtgtcgaa ataggccttg gcaccttcca ccgccacctc ccgcagcaac ccggccgcgg
      361 taaactggtg cagcgtgttg tagacggttg cgagcgaaac gcgcaccttc cgcgccaagg
      421 cctcctcatg cagcatctcg gccgtgacgt gacgatcgtc accggcgaag agcagttggg
      481 cgagcgccac gcgctggcgc gtgggcctga ggcccgcctc ccgcaggatt ctggcaatgt
      541 ctcccagcgt tggctcggct ggcaatcgcg aaaggaccga aggcgaccga tgcatctaaa
      601 tgaccatgtc cgacaaacct tggaggaaat ataggcgcaa accccttccc gctgcaacgc
      661 acctgcgctg agctgcgcca acggggccaa atgggggccc ggagatgcgg ttaggggcgt
      721 caatcttagg gccacccgca tgcaatgtgc ccccaagcgc tcgaaatcct tgcgcgaggc
      781 gggcttttga agggcttccg tctgtgttag gaaaccctcc tttggcgggc cgggagtaag
      841 ccacgaccga tgtcagagcg gcgggaccaa ttcgactatg aggaccttct ggcctgcgcg
      901 cggggcgagc tgttcggccc cggcaacgcc cgcctgccgc tgccgcccat gctgatgttc
      961 gaccgcatct gcgaagtctc cgaagatggc ggcgagcatg gccggggccg gatcgtggcg
     1021 gagctcgacg tgaagcccga tctctggttc ttcccctgcc atttcaaggg cgaccccgtg
     1081 atgcccggct gcctcgggct cgacgcgttg tggcagctgc tcggtttctt tctcggctgg
     1141 ctcggcgcgc ccggaaaggg ccgcgctttg agcgtaggcg aggtcaagtt ctcagggatg
     1201 gtgctgccga ccatgaagaa gctcgaatat atcgtcgagc tgaaacgcgt catccggcgc
     1261 aaattcacgc tcggcaccgg cgacggctgg ctgaaggccg atggcgagac tatctacact
     1321 gccaaggacc ttcgggtcgg cctgttccag gaaggcgagg tcgcacccgc cggtgcctga
     1381 aatcttctgc tcgcaggggc gctcgcctcc taggatcttg atccgatgaa acgtgtggtc
     1441 gtgacgggga tgggcatcgt ctcctccatc ggcaacagcg cccaggaggt gctcgcctct
     1501 ttgcgcgaag caaaatccgg catcgtcaag gccgagacct atgccgagct cggcttccgc
     1561 tgccaggtgc atggcgcacc caagctcgac tgggagggga tgatcgaccg caaggtgcgc
     1621 cgcttcatgg gatcgggcgc agcctggaat tacctcgcca tggagcaggc ggtccgcgat
     1681 gcggggcttg aggagggcga catctcaaac gagcgcaccg gcctgatcat gggctcgggc
     1741 ggtccctcga cgcgcgccat cgtccaggcc gccgacgcca cccgcgccaa gggggccaag
     1801 aaagtcggac cgttcgaagt gccgaaggcc atgtgctcga caaactcggc cacgctcgcc
     1861 acgccctacc gcatcaaggg cgtgagctac tcgatctcct cggcctgctc gacttcggct
     1921 cactgcatcg gcaacggcgc cgagctgatc cagttgggca agcaggacgt cgtgttcgcc
     1981 ggcggcgggg aggagctcga ctggacgctt tccgtcctgt tcgacgccat gaacgccatg
     2041 tcctccgatt tcaacgaccg tcccgaagtc gcaagccgcg cctatgacgc cgaccgcgac
     2101 ggtttcgtca tcgccggagg tgcgggcgtg atcgtgctcg aggaactcga acacgccaag
     2161 gcgcgcggtg cccgcatcta tggcgaggtg gccggctatg gcgccacatc cgacggccat
     2221 gacatggtgc agccttcggg cgagggggcg gtgcgctgca tgcgcatggc catgcggaat
     2281 gtcgacggca tcgactatat caatccgcac gcgacctcga ctccaatcgg cgacgtcaag
     2341 gaaatcgaag ccatccgcga ggtgttcggc gacaagtgcc cgccgatcag cgccaccaag
     2401 tcgctcaccg gccattcgct tggcgccgcc ggcgcgcacg aggcgattta ctcgctgctg
     2461 atgatgaata acggtttcat ttgcgaatcc gccaatatcg agaccatcga ccctgcggtt
     2521 gccgatatgc cgatcgtgcg caaacgtcag gacaatgtct ccattgacag cgtgatgtcg
     2581 aacagcttcg gcttcggcgg caccaacgcc accctggtct ttcgccggct ggatggctga
     2641 gacgatagga gtgaggtggg tttaacccgt aaccatagta ggcatcatga caacaacaag
     2701 agatatcgct aagcctgcgc cgttgatggc agggaagagg ggactggtga tgggggtcgc
     2761 gaacgaccgc tcgatcgcct ggggcattgc gcgggtgctg cacgggcagg gcgccgagct
     2821 tgcgttttcc tatcagggcg gggcgttcgg acgacgcgcc gtcccgcttg ccgaatcgat
     2881 cggctccaag atcatcgtgg aagccgatgt cgaggatctg aagagcgtcg agcgtgtgtt
     2941 cgatgcgatc ggcaagacat ggggttcgct cgatttcgtc gtgcatgcgc tcgcgttttc
     3001 cgaccgcaat gagctgaagg gccgctactg cgacaccacg cgcaagaatt tcgagcacac
     3061 gatggtgatc tcgtgcttct ccttcaccga gatctgcaaa ttggccgccg gcttgatgcc
     3121 ctcgggcggc gcgcttctga cgctgaccta cggcggttcg tcccgtgtcg tgccctgcta
     3181 caacgtgatg ggggtggcca aggcggcgct cgaggcgagc gtccgctatc tcgcctccga
     3241 tttcggtccg caagggatca gggtcaacgc tctgtcggcc gggccgatgc gaaccctggc
     3301 cggtgcgggc atctcggatg cgcgcacgct gtttcatttc cagcagcgca acgccccgat
     3361 gcgccgctcg cccacgctgg acgaggtcgg cggcgccgcg ctttatctct tgtcggatct
     3421 gtcaggcgcg gttacgggcg aggtgcattt cgtcgattgc ggctattcga ccatcgccat
     3481 gccgaacatt tccgcgctca agacgatgga acaggccgag gagggagagc gcgcgaccga
     3541 gaaggtgccg ccgcgcgagg ccgctcagta acggtaacgg ttagtcgagc cgatattgac
     3601 agggcccggc cgcccaacaa tgttacgcgc tcgcagcaag atccggcctt ccggcgcgcg
     3661 ataagtgcga cgctcaaggc cgcaagaagg agaatggcat ggccgaactc attgtcatgg
     3721 tgtgtctcct ggccacgccc gagaagtgcc aggaattcac ggtgcccgga gcctcgaccg
     3781 gggacgtggt gagctgcatc aggcagggcg gcgacaaatc caccgcgtgg cagacgcaga
     3841 acagccaata cttcgtcatc ggctggcgct gcgtgatgaa caagactgcg cagtagaaat
     3901 cctacttcgg gacacgcatg gcgaagatgc ttgcagcgtg cttctctcgg gttcgaggtc
     3961 ttagccagtc tcgatcagga actttccctt ttcgtcattg ccgggcttga tccccggatc
     4021 aagtccgggg acaggctccg gcaatccatt ccagtgacgc ttcaacgcgc tgacgcgtaa
     4081 cggcatgtct gcccgggtca agcccgggca tgacgaggaa taagaaacgg ctggactacc
     4141 ccgcttcggc cctcgcgatc tcctttccgg tcttctgatc gacgaccttc atcgacaacc
     4201 tgaccttgcc gcgatcgtcg aagccgagca gcttcaccca gacctcgtcg ccttccttga
     4261 ccacgtcgtt gacggtcttt ggccggccgt cggcgagttg cgagatatga acgagcccgt
     4321 cgcgtgggcc gaagaaattc acgaaggcgc cgaactccat caccttgacg accttgccct
     4381 tgtagatctc gccgacttcg ggctcggcgg cgatcccctt gatgcggttg atcgcggccc
     4441 gaatcgactc ggcattggcc gaggcgatct tcaccgtgcc gtcgtccgag atgtcgatct
     4501 tggctcccga ttccgccacg atctcccgga tcaccgaacc gcccgaaccg atcacctccc
     4561 tgatcttatc gaccgggatg tggatcgtct cgatgcgcgg cgcgtactcg ccaagctctg
     4621 gtctcgccgt gttcagcgcc ttgttcatct cggcgaggat atgcatgcgg ccgtcgcgcg
     4681 cctgatcgag cgcgaccttc atgatctcct cggtgatgcc ggtgatcttg atgtccatct
     4741 gaagcgaggt gacgccctcg gaggtgccgg cgaccttgaa gtccatgtcg ccgagatggt
     4801 cctcgtcgcc gaggatgtcc gacaacacgg caaaatcctc gccttccttg atcaggccca
     4861 tggcgatgcc cgccaccgaa cgcttgagcg gaatgcccgc gtccatcatc gcgagcgatg
     4921 aggcgcacac cgttgccatc gaggaggagc cgttcgactc tgtgatctcc gacacgatgc
     4981 ggatggtgta gggaaagctc tcgcggtccg gcaaaacggg gtggatcgcc cgccaggcaa
     5041 gcttgccgtg gccgatctcg cgccgtcccg gagacgtcat tcgcccgact tcgccgaccg
     5101 agtagggcgg gaagttgtag tgcagcatga aggtctcttt ataggtgccc tggagcgcgt
     5161 cgacatattg ctcgtcctcg ccggtgccga gcgtggcgac ggcgagcgcc tgggtctcgc
     5221 cgcgggtgaa gagcgcgctg ccgtgcgtgc ggggcaaaat gccgacctcg cagacgatcg
     5281 gcctgaccgt ggtcaggtcg cgcccgtcga tgcgcgtctt gctgttgagg atgctcgagc
     5341 ggacgatctc cttctcggcc tccttgaagg cgcttgcgac cttggccgcg agcgtcagat
     5401 cgtcatcggc gggaacgagc gctgcgacca ccttgtcctt gagggcggcg actcgatcgc
     5461 ggcgttcgtg ctttccggct atcacatagg cctcgcgaag ctcgctttcg ccgagttcgc
     5521 ggacgcgcgt caccaactcg ccctcatcct tcggcttgag ttcccaaggc tcctttgcgg
     5581 agcgctcggc cagcttgacg atggcgtcga ggacaggttg gaagtgcttc tgcccgtaca
     5641 tcacggcgcc gagcatcacc tcttccggca actccttggc ctccgattcg accatcaaca
     5701 cggcgtcggc ggttccggcc accacgagat cgagcgcgct ctcgggcatc tcgtcgatca
     5761 tcggattcag cacgtactgg ccgccgatat agccgacgcg cgcagccccg atcggcccga
     5821 ggaaggggac gcccgaaagg gtgagcgccg cggaagtcgc caccatcgcc acgatatcgg
     5881 gatcgttctc catgtcgtgg gacagcacgg tcgccacgat ctgcgtctcg ttcctgaagc
     5941 ctgaggcgaa aagcggccgg atcggccggt cgatcaggcg agacgtcaat gtctctttct
     6001 cggtggggcg accttcgcgc ttgaaatagc cgcccggaat cttgcccgcg gcgaaggtct
     6061 tctcctggta gttcacggtc agcgggaaga agtcgatgcc gggcctctct tccgtggcgg
     6121 caacgacggt ggcgagaacg ctggtctcgc cataggtcgc gagcacggcg gcatcggcct
     6181 gacgggcgat gcggccggtc tcgagtttta gggggcgatt cccccattcg atctctacgg
     6241 tctctctctt gaacatggtg gtgtcccaaa tgcgtaggca ggggtcgagc ggctcctttt
     6301 gccaaggggc gaaagggctg ccagttcgga attcgcaagc tcgatcgcgg gctcacccgc
     6361 aagcgcgagt gtcaccggcg gatgcccaaa cggttgatga gtttctggta acggtcttcg
     6421 tcggccgact tgacgtaatc gagcaggcgc cgccgctggc tgaccatctt gagcagcccc
     6481 cggcgcgaat gattgtcttt gccgtgcgtc ttgaaatgct cggtgagatt ggtgatacgt
     6541 tcggtcagga ttgcgacctg gacttctgga gatccggtat cgccggattt ggtcgcgtat
     6601 tcgttgatga gctcttgctt gcgctcagcg ctgatcgaca tcggatgatt ccttgcggtt
     6661 ttacggttga cggtggccgg gccgggatgt cgtccagcac ggtcggacaa aagccgcttg
     6721 ggcgctttgc gcccgcgcga atggcggcaa tatagggata atccgtcacg aggccagcgg
     6781 aatttacccc tctcccctgt gggagagggc ttcccgcccg gcgcgcgaag cgcgacgaat
     6841 ggcggaaagg gtaaggggtg actcgtcttg acgcgggctc acgcccgctt agccctcacc
     6901 gcgcgccaaa cggcgcgcgt gctctcccac aaggggagag cgggaacctg ttggcagatt
     6961 gaagatgcgt ctcggatgga gctcgccacg ctcgacctcg ccgagcgcaa ccagcgtgcc
     7021 gcgcgaggtg gcatagacgg tgccttcgac gatcggcgca tcgcggccgc gtaacagaac
     7081 ggcttgtccc cgccgaagcc tgtcagcgtc ttgagcggaa accgaaagcg ccggcattcc
     7141 ttcgagcgcc gtctcgactg gctcgagcgc gggcataagc gcctcgcgac cgccctcttc
     7201 ggcgcgtgct ccgagcgccg cgagcgcaac ggcccgatcc tcggcgaagg ggccgacccg
     7261 ggttcggcgc aaggcctcga cgtgaccgta gcagccgaac gcgcggccga gatcgcgtgc
     7321 gagcgcccgc acataggtgc ccttgccgca acgcgcctca agcagggcgc ggtccttgtc
     7381 gggatgttcg atcagcctga gccagtcgac cgacacgcgc cgcggctgaa gcgcgaaaat
     7441 ctcctcgtcg cgggcaagat cgtaggcgcg ggcgccatcg accttgagcg cggagtagcg
     7501 cggaggtacc tgctggatct ccccggtgaa gtcggcaagc cccgcctcga tctcggcgca
     7561 cgatggtcgg agatcgcttt gcgcgacgac ctttccctcg gcgtcgtcgg tgtcggtctc
     7621 cgccccgaag cggacggtga agcgatactc cttgcagctc tcgaccacga aggggaccgt
     7681 cttggtcgcc tcgccgagcg caattgggag cacgcctgtc gcgagcggat cgagcgtgcc
     7741 ggcatgcccc gccttcgccg cctggtagag gcgcttgatc ctggaaacgg cctcggtcga
     7801 ggtcatgccc gcgggcttgt cgaggatgag ccagccatgg acgggaaggc ctttgcgctg
     7861 ccgcgccatt gctcctccat gcgctcccgg ctagccgtcg aggtcgcgag cgactttagg
     7921 cgaggcgaga agctcatcga tgcgggacgc cgcctcgaac gaggtgtcca ccttgaatct
     7981 gagctccggc atgaacttca cgctgatccg cttcgccagg agcccgcgca gatagcgctt
     8041 gtgcttgtcg agatgggcga ccgcctcctc cgcctcgccg ccgccaagcg tcatcacata
     8101 gaccgttgcg agtttgaggt cgggcgacat gcgcacctcg ggcaccgtga gcgagtgccg
     8161 gttcaacacg tcgtcggcga tcgcgccgcg ggcgaagatc tcggccagcg catgccggat
     8221 catctctccg actttcagct ggcgttgcga gggcggcttg gctttgtcgc gatggctcat
     8281 tctgctgtcg ttacttctca gcgtcattcc ccgcctgcct gcgcaaagcc ggagcgcctg
     8341 cgcactgaat ctccaccgag aaacggcttc gcttcgcgaa ggcaggaaag cgggaatcca
     8401 gtaaccacca aagctgcgat gaagcaacga acctcgccgt ttactggatg cccgccgccg
     8461 cgggcatgac aggaagggat cagagcgtgc gcttgacgtg ctcgacctgg aagcactcga
     8521 tgacatcgcc ctgccgcatg tcctgatagc cttcgaaggc catgccgcat tcctggcccg
     8581 ccaccacctc gcgcacctcg tccttgaagc gcttgagcgt ggagagcttg ccctcatgca
     8641 cgacgacgtt gtcgcggatg agcctgactt gcgcgccgcg ctcgaccttg ccctctgtca
     8701 cgcggcagcc cgccaccttg cccaccttgg agatggtgaa tacttcaagg atttcggcat
     8761 tgccgaggaa cgtctcccgc acggtcggcg cgagcagacc cgacatggcc tgcttcatgt
     8821 cgtcgaccag atcgtagatg acggcgtagt agcggatctc gacaccctcg cgctcggcca
     8881 cctcccgcgc ttgcgcgttg gcgcgcacat tgaaaccgag cacgaccgcg cccgaggtcg
     8941 ccgccagcga cacgtccgat tcggtaatgc cgccgacgcc tgcgtgaatg aggcgcgccc
     9001 tgacctcgtc ggtgctgacc ttggcgagcg ccgtggtgat agcttcgacc gagccctgca
     9061 cgtcgccttt gacgaggagg ggaaactccg cgatgccggc ctctttcagc tggttcatca
     9121 tttgctcaag gctgccgcgg gcgccggcga ccgcgagccg cttacgccgc tcgcgcacgc
     9181 ggtagtcggt aatctcacgg gcgcgggcct cgttctcgac cac
//
LOCUS       DASUQP010000109         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1958153, whole genome shotgun sequence.
ACCESSION   DASUQP010000109 DASUQP010000000
VERSION     DASUQP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958153"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            159..1892
                     /gene="thiC"
                     /locus_tag="VFY74_04890"
     CDS             159..1892
                     /gene="thiC"
                     /locus_tag="VFY74_04890"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX5931073.1"
                     /translation="MNKPVPPNDVASPELTTGPLPGSRKVYYSPDGHRGLRVPFREIA
                     LSAGSGEPPFRLYDTSGPYTDPDAAIDFERGLPKLRAPWIAARAAAGKPVTQIELARA
                     GIVTEEMVFIAHRENLGRASAAEATCARLAGGESFGASLPSFVTPEFVRSEVARGRAI
                     IPANINHPESEPMIIGRNFVTKINANIGNSAVTSSIAEEVEKMVWAIRWGADTVMDLS
                     TGRNIHATREWIIRNAPVPIGTVPIYQALETVGGDPVKLDFEVYKDTLIEQAEQGVDY
                     FTIHAGVRLGYIHLTANRVTGIVSRGGSIMAKWCLAHHRESFLYERFDEICEIMRRYD
                     VSFSLGDGLRPGSIADANDHAQFAELETLGELTRVAWDHGCQVMIEGPGHIPMHKIKV
                     NVEKQLAICGEAPFYTLGPLVTDIAPAYDHITSAIGAAMIGWFGTAMLCYVTPKEHLG
                     LPNRDDVKAGVIAYKIAAHAADLAKGHPTAVARDDALSRARFAFRWEDQFSLSLDPET
                     ARSFHDETLPKEAHKLAHFCSMCGPKFCSMEITQQVRDYAAKLAEKERGMEEMSAKFR
                     ASGAEVYVARD"
     gene            complement(2341..>2645)
                     /locus_tag="VFY74_04895"
     CDS             complement(2341..>2645)
                     /locus_tag="VFY74_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931074.1"
                     /translation="TAFETFDLRGSLAHEINRRILTYRGKLLGEADEMDLPAIGLDPT
                     GTSMRVPALLAHGVLSDAQFGSVKKFNSRGSHNRGRARNSPARGFAGADSAIGVLG"
BASE COUNT          496 a          845 c          819 g          485 t
ORIGIN      
        1 aggggagccc aggcaagggg ctgagagtcc gctgtctcaa accgaggccc ggcggagacc
       61 cttcgaacct gatccgggtc atgccggcga agggacggga ctttcctcat ctccgtttcg
      121 ccggcatctc cctcgcttga agccaaggga gcatgacgat gaacaagccg gttcccccga
      181 acgacgtggc ttcccccgaa ctgaccaccg ggcccctgcc ggggagccgg aaggtctatt
      241 actcacccga cggacaccgg ggcttgcgcg tgcccttccg cgagatcgcg ctctcggccg
      301 gaagcggcga gccgccgttc cgcctctacg acacgtcggg accgtacacc gaccccgatg
      361 ccgcgatcga tttcgagcgc ggccttccaa aactcagagc gccgtggatc gccgcgcgcg
      421 ccgctgccgg caagcctgtg acgcagatcg agcttgcccg cgccgggatc gtcaccgagg
      481 aaatggtctt catcgctcat cgcgagaatc tcgggcgcgc aagtgcagct gaggcaactt
      541 gcgcgcgcct tgccggcggc gagagtttcg gcgcgagcct gccctcgttt gtgacgcccg
      601 aattcgtgcg gagcgaagtc gcgcgcggcc gcgccatcat cccggccaat atcaatcacc
      661 ccgaatccga gccgatgatc atcgggcgaa acttcgtgac caagatcaac gccaatatcg
      721 gcaactcggc ggtgacttcc tcgatcgcgg aggaggtgga gaagatggtg tgggcgatcc
      781 gctggggcgc cgacacggtg atggacctct cgacggggcg caacatccat gcgacgcggg
      841 agtggatcat ccgaaatgcg ccggtgccaa tcggcacggt gccgatctac caggcgctcg
      901 agacggtcgg cggtgacccc gtgaagctcg acttcgaggt ctacaaggat acgctgatcg
      961 agcaggccga gcagggtgtg gactatttca ccatccatgc cggggtgcgg ctcggctaca
     1021 tccacctcac ggcgaaccgc gtgacgggaa tcgtgtcgcg cggcggatcg atcatggcca
     1081 aatggtgcct tgcccatcac agagagagct tcctctacga gcgcttcgat gagatctgcg
     1141 agatcatgcg ccgttacgac gtgtcgttct cgctcggcga cgggcttcgc ccgggctcga
     1201 tcgccgatgc caacgaccac gcgcagttcg ccgaactcga aacgctgggc gagctgacgc
     1261 gcgtcgcctg ggaccacggt tgccaggtga tgatcgaagg gccgggtcac atccccatgc
     1321 acaagatcaa ggtcaatgtg gagaagcagc tcgcgatctg cggcgaggca ccgttctaca
     1381 cgttaggccc gctcgtcacc gacatcgcgc ctgcctacga ccacatcacg agcgcgatcg
     1441 gcgcggccat gatcggctgg ttcggcacgg ccatgctttg ctacgtcacg ccgaaggagc
     1501 atttgggcct tcccaaccgc gacgacgtca aggcgggcgt catcgcctac aagatcgcag
     1561 cgcatgcggc cgacctcgcc aagggccacc cgacggcggt cgcccgcgac gacgcgctgt
     1621 cacgcgcgcg cttcgcgttc cgctgggagg accagttcag cctttctctc gatccggaga
     1681 cggcgcggag cttccatgac gagacgttgc ccaaggaggc gcacaagctc gcccatttct
     1741 gctcgatgtg cgggccgaaa ttctgctcga tggagatcac acaacaggtg cgcgactacg
     1801 ccgccaagct cgccgagaag gagaggggca tggaagagat gagcgcgaaa ttccgcgcga
     1861 gtggcgcaga ggtctatgtg gcgagggatt aggctgattt cgttcgttcc gcgggcgtgc
     1921 acttgccgga gagcccttta gctttcgtct aaagctaggt gccgatctct ggtgatccgc
     1981 tgggcccact ctttgtaaag agggtcgaaa agcgtatcga tctggaattc tagcgagccg
     2041 aaagtcacgt gactgtgctg ttttatacgg gggaaacttg tcaaccgacc ttacagaccc
     2101 cggctcgctt cggcaggcgc cctttgcttt tctcccatgg cgttgcccaa atgcgacact
     2161 ccaaggagcg tggttgtgtt atcaacaatc ttctgatggc caaatggtca gcagtagaaa
     2221 gccatctcac cgttccaact aagagccccg gtgcttcccc tccggggctt tcccctttgc
     2281 ggtggcccaa cggaaccctg cgcccactgc acgcgtttcg ctccctagat ttcggcgagc
     2341 tcaccccaga acgccaatcg ccgaatctgc ccctgcaaag cctcgcgccg gtgagttccg
     2401 ggccctcccc ctgttatggc tgccacggct gttgaatttc tttacgctgc cgaattgcgc
     2461 atcgctgagc acgccgtgcg caagcaaggc tggcacgcgc atggacgttc cggttggatc
     2521 aagcccgatg gcggggaggt ccatttcatc tgcttccccg agcagcttgc cgcgataggt
     2581 aaggatgcga cggtttattt cgtgggcgag cgatccccgc agatcgaacg tttcaaacgc
     2641 agttg
//
LOCUS       DASUQP010000110         3989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1548959, whole genome shotgun sequence.
ACCESSION   DASUQP010000110 DASUQP010000000
VERSION     DASUQP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548959"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFY74_04900"
     CDS             <1..704
                     /locus_tag="VFY74_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5931075.1"
                     /translation="RAEALEQEVLACQETVASETAIAGRVLVTATETLASAFVLRHLP
                     ALRSAYPSLRVELVRTEQTLNLSRREADIALRLARPHQLDLRVRRIARLDFGLYASPA
                     WIEQFGSPRESSDLKNCDVIDWVDKRPDYPAIRWFTQATDASRIIFRANSPSDRLTAA
                     REGIGVALGPCLVGDTDPGLVRLLPELELVGPEVWLLVHRELADLARVRVVLDVLAQC
                     ARADASRFAGRTRLS"
     gene            785..1318
                     /locus_tag="VFY74_04905"
     CDS             785..1318
                     /locus_tag="VFY74_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX5931076.1"
                     /translation="MRQRTIVIAAMILLSSTPAAAELRIPCLWNPFGGCSVTLVSPDY
                     AKRPVQPLNREAALAEVNAFRAANGRDALILDDRLCRAAAMQSETQAARSAIGHTGPG
                     GSKPMDRAARAGYRAKIASENVASGQKSFSDALRTWKGSFSHRTNMLRPNVSAIGVAM
                     AKNENGRAYWTLVLAGE"
     gene            complement(1397..1867)
                     /locus_tag="VFY74_04910"
     CDS             complement(1397..1867)
                     /locus_tag="VFY74_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931077.1"
                     /translation="MRKLSAVAVMASIGASLFACTERPPSQINSTAANSMAYERPVPV
                     TRTPLAPPAGYGQGYASPPPLGTSQPPPLYSNSPPPSPLYGNFPHDDADPHMAELGWR
                     ASPRWSAIEGDGCIEVEQGGETGYGAQMKIERCAKQAPRGKAPAPAETMAPPDY"
     gene            2965..3786
                     /locus_tag="VFY74_04915"
     CDS             2965..3786
                     /locus_tag="VFY74_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX5931078.1"
                     /translation="MELAEIHCYPVKSLRGHALKTATVEPIGLKGDRRWLVVDASGRF
                     RTIRELPKMVQIEVEATPLGIRMIHGEAGSCTVDAPHEGTDECDITIWRDTVRAAAAG
                     RDADVFLSRVLGTKVRLVYLADPRGRPLGAKSGAAKDHVNFADDFPVLLTSRSSLADL
                     CKRAGADIAMRRFRPNLVIAGAAPWEEDRWRAIRIGTVRFRVGAPCERCVITTRDPDT
                     GEQIDPYEPLRTLMRFHRARNGSAIFGQNLIPDNPGTVSIGDTVEVLSAGASNVL"
BASE COUNT          732 a         1193 c         1324 g          740 t
ORIGIN      
        1 ctcgtgcgga ggcgttggag caagaggtgc ttgcgtgtca agaaacagtc gcaagcgaaa
       61 cggccatcgc agggcgggtg ctggtcactg cgacggagac cctcgcaagc gcgtttgtgt
      121 tacggcatct tcccgcgctc agatcggctt atccatcact gcgcgtcgaa ctggttcgga
      181 cggagcagac gctcaatctg tcgcggcgcg aggcggatat tgcgctgcgg cttgcgaggc
      241 cgcatcaact cgatctgcgc gtgcgacgga tagcgcggct tgactttgga ctttacgcgt
      301 ctccggcttg gatcgagcag ttcggttcac cacgagagtc gagcgacctc aagaattgcg
      361 acgtgatcga ctgggttgat aaacgtcccg attatccagc gatccgatgg ttcacccagg
      421 cgacagacgc gagccggatt atctttcgtg caaactcgcc ctccgatcgc ctgactgccg
      481 cgcgggaggg gataggcgtc gcgcttggtc cttgcttggt aggtgataca gaccctggtc
      541 ttgtgcgcct gctaccggaa ttggagctcg tcgggccgga ggtctggcta ctggtccacc
      601 gcgagcttgc cgacttggcc agggtccgtg tggtcctgga tgttttggcc caatgcgccc
      661 gtgccgacgc gagccgcttt gcagggcgga cgaggctgtc ttgagacagg accctgttat
      721 tgtctcgcgg tgtttggaca ggggcgccgc cttggcgcta tgctgaatgt ttcacggggg
      781 acccatgcga cagcggacca tcgtcatcgc cgccatgatt ttgctttcgt cgacgccggc
      841 agcggccgaa ctgcggatac cgtgtctgtg gaacccgttc ggcggctgtt ccgtgaccct
      901 cgtctcgccg gactatgcca agcgtcccgt gcagccgctc aatcgggaag ccgcgctcgc
      961 ggaggtcaac gcgttccgtg ccgcgaacgg acgcgatgcg ctcattctcg atgatcgcct
     1021 ttgccgcgct gcggccatgc aatccgaaac tcaggccgcg cggagcgcga tcggtcacac
     1081 tgggcccggc gggtcgaaac ccatggatcg cgccgcgcgt gccggctatc gtgcgaagat
     1141 cgcctcggag aacgtcgcct ccgggcaaaa atcattcagc gacgcgctcc gcacctggaa
     1201 gggaagtttt agccatcgca ccaatatgtt gcgtcccaat gtcagcgcca tcggcgtggc
     1261 gatggcgaag aacgagaatg gccgtgccta ctggaccctc gttcttgccg gcgaatagac
     1321 gcgagtcaga tcttggcgag cttagcgcgg ggtgtcaggg cgcgaccgcg ggccggcgat
     1381 ccacgcagcc tcaacgctag taatcgggtg gcgccatggt ctcggcgggg gccggggcct
     1441 tgccgcgggg cgcctgtttc gcgcacctct caatcttcat ttgcgcgcca tacccggtct
     1501 ccccgccttg ctcgacctcg atgcagccat ctccttcgat cgccgaccag cgcggcgaag
     1561 cgcgccagcc gagctcagcc atatgcggat cggcgtcgtc atgggggaag ttgccataga
     1621 gcggcgaggg cggcggtgaa ttactataga gcggaggcgg ctgcgacgtg ccgagagggg
     1681 gcggcgacgc atagccctgc ccatagccag cagggggcgc cagaggcgtg cgcgtgacag
     1741 gaaccgggcg ctcgtaggcc atcgagtttg ccgccgtgga attgatttga gaaggcgggc
     1801 gctcggtgca ggcgaagagc gaagccccga tcgaggccat gaccgcgacg gcagagagtt
     1861 tccgcattcc gtccccccgg ggacttgcaa cctgaaccaa ggcgcaagcc taagccctag
     1921 gggttaccag aaggtcacgc tcggcttggc atcggcggga gcgtggcagc cagttcgtta
     1981 cgaaacctag ggccgtacgt cgcgcagagc gaaacccgct cttcggctaa gaacgcgggc
     2041 gatcgggacc agggggcgcg aaatcgacgc cgaaaatcaa gagtatggcg agggctttga
     2101 cgagatgggc catcatggcg tgtctcccca ggccggagaa acctccggta ggaaattgac
     2161 gtatggcgtc gcggcgtgaa gtacaaatca cattttccta tcagataatg ttttttactg
     2221 ctggtgaagc cacattgggc tccgtcggtt caagaaggtg gagggcgagg acgtgcgaaa
     2281 gccaaaaggc gattaaaaag cgagcctaat ctgtctcgtt ccaagacgat ttcgtcagcc
     2341 tgctgatagc cataggtcga gcgtgcgcta tctcgccatc ctgcacaggc tagagtcgcg
     2401 actgggcgga catcaccagg caatactgcc cggctgttgc ggtttcgacg ttcgcccaac
     2461 gtgaatagca gcggggcgat cctttgcctc ctgagcgcgc ctaaagattt cagaccagct
     2521 aggaaaaagg acaaggccgc ctcgccggcc ttgtccttga cgtgcatcgg cgcgacggtg
     2581 acggttaggg cggagtccgc gagtgcaagc ggccccacgc cgcggagccg cttgcgactt
     2641 ggttgcgcgc gtgcggggga ggcatgaccg cgccataaaa gatgcgccga acttcgtcgc
     2701 ccacgcatgc tgagcccata cgccgagcga gacacgcgcc aacgtgccgt catggtcgcg
     2761 tcgccgctct gcccgcatga tttgttccgc cgcaagcgct ccgcttgcct cgaccgtagc
     2821 ctcgtcgtct cctcttcctt agaacaacgg tgttgtccaa gatgcgcgtg tgaagcgccg
     2881 ccgggccgat aagctcggca ggggacggct tggccgcgat cagtcatgtt ggaatttgac
     2941 tcgcggcagg atgaggcatg acagatggag cttgccgaaa tccactgtta tccggtcaag
     3001 agcttgcgcg ggcatgcact caagacggca acggttgagc cgatcggcct caagggcgat
     3061 cgccgctggc tcgtcgtgga tgcttcgggg aggtttcgga cgatccgcga gctcccgaag
     3121 atggtgcaga tcgaagtcga agcgacgcct ttaggcatcc gcatgatcca tggcgaagcg
     3181 ggctcgtgta ccgtcgacgc tccgcatgaa ggcacggacg aatgcgatat cacgatttgg
     3241 cgcgacaccg tgcgggctgc ggcggcaggc cgtgatgcgg acgtgtttct ctcccgcgtg
     3301 ctgggcacga aggttcggct tgtttatctc gccgacccca gaggccgccc actcggtgca
     3361 aagtccggcg cggccaaaga ccacgtcaac ttcgccgacg acttccccgt gctgttgacg
     3421 tcgcgaagct ctcttgccga tctctgcaaa cgcgccggcg cggacatcgc gatgcgccgg
     3481 ttccggccaa acctcgtgat cgccggcgcc gcgccgtggg aggaggacag gtggcgcgca
     3541 atcaggatcg gaacggtccg gttccgggtt ggagcccctt gcgagaggtg cgtgatcacg
     3601 acgcgcgacc ctgacaccgg cgagcaaatc gatccttacg agcctttgcg cacgctcatg
     3661 cgatttcatc gcgcccggaa tggcagcgcg atcttcggcc agaacctgat ccctgacaat
     3721 ccgggcacgg tcagcatcgg cgacacggtc gaagtgctct cggcaggcgc ctcgaatgtg
     3781 ctgtgaggca gcaagtcggc ggacgcgggt gggtaagcgc ggcaattctt ctggcgcacg
     3841 ctcgcaaagt cgtggatggc cgggacaagc ccggccatga cgaagggggt ggatggcaga
     3901 cgtgatgtga caatgaagca atcgttttca atggcttagc gcgattcgat cgaaaatcct
     3961 tgcgcctcga aggcgggggc ggcctcggg
//
LOCUS       DASUQP010000111        13727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1004834, whole genome shotgun sequence.
ACCESSION   DASUQP010000111 DASUQP010000000
VERSION     DASUQP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13727
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004834"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFY74_04920"
     CDS             <1..559
                     /locus_tag="VFY74_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5931079.1"
                     /translation="EVLEAAEYFSEGQKGSSAMPHKRNPVLSENLTGLSRLVRGYVMP
                     ALENVALWHERDISHSSVERVIGPDATIALDFALTRMTSIIKDLEVYPERMQENLNRL
                     GGLIHSQRVLLALTEAGVPREEAYRLVQRNAMKVWKDGAEFLDALLADKDVTKALSPD
                     EIRAQFDLGYHLKHVDTIFKRVFGD"
     gene            complement(601..942)
                     /locus_tag="VFY74_04925"
     CDS             complement(601..942)
                     /locus_tag="VFY74_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931080.1"
                     /translation="MRIIFACALLLALAGFAQPAHADGYRGYREYIPVYPRYPYRSGH
                     YPKADWYYQLPPYAAIHPSFYRRRFFMMAQERNRYRYMPHIFHGYSEGDQIEDIDIQG
                     ISSTLRGTYDD"
     gene            complement(999..1760)
                     /locus_tag="VFY74_04930"
     CDS             complement(999..1760)
                     /locus_tag="VFY74_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5931081.1"
                     /translation="MREDRFRRAAKERRTLFNAWLTLNNPFLIELIGEAGWDCITIDQ
                     QHGLGGNEALVGCLTAARACGLPAIVRVAENSPGLIGRALDAGAQGIMCPLIGNVEDA
                     EAFVQAVKFPPRGNRSWGPYRARLDHPGADYFKIANDWTIACPQIETRGALDQLDEIL
                     ALPGVDMVCFGPNDLSVALTGRLDIWAAEVKDAMTLVLSKCRERSVIALIFANDVDYA
                     KPLVKDGWDVVAVGTDAGWFSKAAAEAKAAAKDNS"
     gene            complement(1769..2098)
                     /locus_tag="VFY74_04935"
     CDS             complement(1769..2098)
                     /locus_tag="VFY74_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEX5931082.1"
                     /translation="MTTFEERKKGFERKFAHDEELKFRATARRNRLLGLWAAGQMGVT
                     GDDATAYAREVVKADLAEPGDEDVFRKIRADFDAKGVGQSDHQIRRMMTELMGEAIAQ
                     IESESKG"
     gene            2407..3177
                     /gene="purC"
                     /locus_tag="VFY74_04940"
     CDS             2407..3177
                     /gene="purC"
                     /locus_tag="VFY74_04940"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5931083.1"
                     /translation="MNRRRRVYEGKAKILYEGPEPGTLVQHFKDDATAFNNKKHDKIE
                     GKGVLNNRISEYLFQRLGEIGVPTHFIKRLNMREQLIREVEIIPLEVVVRNVAAGSLS
                     TRLGMDEGTALPRSIVEFYYKNDQLGDPMVTEEHITAFGWATPQEIDDVMSLALRIND
                     FLSGLFLGVGIRLVDFKCEFGRQWENEQMRIVLADEISPDCCRLWDIETQNKLDKDRF
                     RRDMGGLVEAYQEVARRLGLHVDNPMRARTGPVLVRSK"
     gene            3195..3440
                     /gene="purS"
                     /locus_tag="VFY74_04945"
     CDS             3195..3440
                     /gene="purS"
                     /locus_tag="VFY74_04945"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5931084.1"
                     /translation="MKARITITLKPGVLDPQGKAIAGALHALGFGSVGSVRQGKYIEV
                     EVAESDPSRARAEVERMCEQLLANTIIENYAYELEAA"
     gene            3830..4519
                     /gene="purQ"
                     /locus_tag="VFY74_04950"
     CDS             3830..4519
                     /gene="purQ"
                     /locus_tag="VFY74_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5931085.1"
                     /translation="MKASVIVFPGSNCDRDAAVALEAATGHAPAMVWHGDSDIPPSDL
                     IVLPGGFSYGDYLRSGAMAAHSPVMREVAARARVGTPVLGICNGFQVLTEAGLLPGVL
                     MRNATLKFVCRDVFLRVERNDSIFTHRYAQGEVVRFPIAHKDGSYFADDATLDRLEGE
                     GLVAFRYCDAAGAITDPANPNGSRRNIAGIYNATGTVLGLMPHPERLADPALNGTDGA
                     KMFLSLVETLH"
     gene            4516..6741
                     /gene="purL"
                     /locus_tag="VFY74_04955"
     CDS             4516..6741
                     /gene="purL"
                     /locus_tag="VFY74_04955"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX5931086.1"
                     /translation="MNIAARAAKAAGRITPEIVAEHGLGQDEYKLMVAALGRDPSLTE
                     LGIFSVMWSEHCSYKSSKVWLRKLPTEGPQVIQGPGENAGVVDLGDGQAAVFKMESHN
                     HPSYIEPYQGAATGVGGIMRDVFTMGARPIANMNVLRFGAPDHPKTKHLVAGVVSGIG
                     DYGNCMGVPTVGGETNFDPRYNDNILVNAMCVGLADAGRIFRSAAKGIGHPVVYVGSK
                     TGRDGIHGATMASAEFDDASEEKRPTVQVGDPFTEKLLLEACLELMAEDAIVAIQDMG
                     AAGLTSSSVEMADKGGVGIELELDRVPTREDGMTAYEMMLSESQERMLMILKPGSEKI
                     AERVFRKWELDFSVIGYTTDTGHLVVRHRGAVEADVPLAALAHAAPVYERPWTKTEPQ
                     ETVVPEAVPAPNSQLGALECLMGSPALCSRRWIWQQYDHMVMADTVQRPGGDAAVVRV
                     HGTRKGLAISCDVTPRYCAADPFEGAKQAVAECWRNLTAVGADPLAITDCMNFGNPER
                     PEIMGEFAGAIEGMAEACTALAFPVVSGNVSLYNETNGVAIPPTPSIGGIGLIPDIAT
                     MATIALKRPGDVLMLLGAEAGHLGQSLYMQVMLNRTFGAPPPVDLDAERRTGDLVRGL
                     IRSGALSAAHDVSDGGLLVAIAEMALAGGLGVALDATPAPLPPHATWFGEDQARYVIA
                     ASPDKADAIHKAAAAAKVPLRLLGSVGGDAITLDGESALPLAALWAAHESWLPRFMAH
                     G"
     gene            6768..6998
                     /locus_tag="VFY74_04960"
     CDS             6768..6998
                     /locus_tag="VFY74_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX5931087.1"
                     /translation="MPMAADDIARLIKERFPDAEVRIEDLAGDGDHYAATIVSEAFRG
                     KTRVQQHQMVYDALKGNMGGALHALALRTAAP"
     gene            7028..7378
                     /gene="grxD"
                     /locus_tag="VFY74_04965"
     CDS             7028..7378
                     /gene="grxD"
                     /locus_tag="VFY74_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX5931088.1"
                     /translation="MSAQDVQDWLRKEIARSDVVLFMKGSKSMPQCGFSMQVAQILNH
                     LGVDFRDVNVLEDMGVREGIKAYSNWPTIPQLYVKGEFIGGCDIVREMFQAGELQELL
                     TSKGIAHNQQTMTA"
     gene            complement(7397..7615)
                     /locus_tag="VFY74_04970"
     CDS             complement(7397..7615)
                     /locus_tag="VFY74_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX5931089.1"
                     /translation="MADMKFSTGNQIKGKILEIRKGAVTAIVYVAVPGDSMLEVSVTI
                     PALETLKLYEGKPVVLMINPGDIIIGCE"
     gene            complement(7630..8832)
                     /locus_tag="VFY74_04975"
     CDS             complement(7630..8832)
                     /locus_tag="VFY74_04975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5931090.1"
                     /translation="MGLVARTLERVKPSPTLAITSKARELKAAGFDVIGLGAGEPDFD
                     TPDNIKQAAIEAIKRGETKYTAVDGIPELKRAVAEKFKRENGLAYKPSEITVGSGGKQ
                     VLYNALLATLDPGDEVIVPSPYWVSYPDIVLLAGAEPIVVETKLEDGFKLKPEALVRA
                     ITPRTKWFIFNSPSNPTGAAYTEPEVKALTGVLVKHEKVWVLSDDIYEHLVYDGFKFT
                     TPAAVEPRLKNRTLTLNGVSKAYSMTGWRIGYGGGPEPLIKAIATLQSQSTSNPSSIS
                     QWAAVEALEGPQNFLKDWVASFQERRDLVVAMLNQAKGLSCPKPEGAFYVYPSCAGTI
                     GKTTRGGKTVGNDEDFATALLEEEGVAVVHGAAFGLSPFFRISYATGIKELEEACRRI
                     QRFCGNLS"
     gene            9298..9915
                     /locus_tag="VFY74_04980"
     CDS             9298..9915
                     /locus_tag="VFY74_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931091.1"
                     /translation="MPLRGSAQLLLLFLRAHSKRGWIMGAIAGGAVAAGVSLGAGAVP
                     LPTAAELAQALRESAWDRALAEAPATSPWPWADVPAATTGKVARLGLSAAMVKEGRSH
                     SMRAVDEPLRPAAAAAPQKSGTMLSKLAVGDRITVTTASGDSRVYRVTGPKVVDPHLA
                     EGGSQAVSGDACVPLDPVLASSLGLVIQAITADPPAPEPEHEQKL"
     gene            complement(9932..10732)
                     /locus_tag="VFY74_04985"
     CDS             complement(9932..10732)
                     /locus_tag="VFY74_04985"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5931092.1"
                     /translation="MTRLVLGSAALAGIFAAVALGLPSHVTVARSVVINAPESAIFPY
                     LNNLHEFGEWSPWSVRDPQLAVSYGGPQTGQGARVTWTSKKPSIGTGSMEISESQPNR
                     HIDLAVDYNGLEGTSAYDIVPSGSGSKVTWTFLYESGSNPLKRWKALMLDGFVGAEYR
                     AGLDRLREKIEAERRPTMPTLSPSVSTQPQTPAAALPGAAALPGAPAPQGAALPPGQA
                     APQPGTAAPGASATPQAGTAPAAQPSTPPAAADATSTAPRKKKRRQQQ"
     gene            10876..11046
                     /locus_tag="VFY74_04990"
     CDS             10876..11046
                     /locus_tag="VFY74_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931093.1"
                     /translation="MTLSAPSTVVFIVSAILWALAVIGNFSQIPFVTENGFWVAVIAY
                     LILAVGNLFRGL"
     gene            complement(12227..13027)
                     /locus_tag="VFY74_04995"
     CDS             complement(12227..13027)
                     /locus_tag="VFY74_04995"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX5931094.1"
                     /translation="MNLLYLSNDVPLWLLTLLLVLVACGYAIGLMLGTRFRYGVDRLS
                     LNNEVAGFKFAVVGVFYAVLLAFVVVAVWEDFRQTEATVRDEAKAAVDLHRITFALPA
                     EAGDEIRKHLIAYTKDVREHEWPAMAVGEPSDVVVKDLDRLTKAIFTVNPATWQELAL
                     YQDALRLLAVMTDNRNERLDSASGSVPGILWIVLIVGGLITLGFPAFFASTNVGAQVL
                     MTASLAVLVAIALLLAIVFDYPFTGAVQVSSLPFEHALREMPSVWPPP"
BASE COUNT         2342 a         4518 c         4427 g         2440 t
ORIGIN      
        1 cgaggtgctc gaggccgcgg aatatttcag cgaagggcag aagggctcct cggccatgcc
       61 gcataagcgc aacccggtgc tgtcggagaa cctgaccggc ctttcgcgcc tcgtgcgggg
      121 ctacgtcatg ccggcgctcg agaacgtggc gctatggcat gagcgcgaca tctcgcattc
      181 ctcggtcgag cgggtgatcg gccccgacgc caccatcgcg ctcgatttcg cgctcacgcg
      241 catgacgtcg atcatcaagg acctcgaggt ttatccggag cgcatgcagg aaaatctcaa
      301 ccggctcggc ggattgatcc actcccagcg cgtgctgctg gcattgaccg aagccggcgt
      361 gccgcgcgag gaggcctacc gtctcgtgca gcgcaacgcc atgaaggtgt ggaaggacgg
      421 cgccgagttc ctcgatgcgc ttctcgccga caaggacgtg accaaggcgc tatcgcccga
      481 cgagattcgc gcccagttcg atctcggcta tcacttgaag cacgtcgata cgatcttcaa
      541 gcgcgtgttc ggcgactagc cctcgcgctg cgcgcgccga gccaagcccg gcattcgtgc
      601 tcagtcgtcg taggtgcccc gcagcgtgct gctgatgccc tgaatgtcga tgtcctcgat
      661 ctggtcgcct tcgctatagc cgtggaaaat atgcggcatg tagcggtagc ggttgcgctc
      721 ctgcgccatc atgaagaagc gccggcggta gaaggaggga tggatcgcgg cgtaaggcgg
      781 aagctgatag taccagtcgg ccttgggata atggcccgaa cgatacggat agcgcggata
      841 gaccgggatg tactcgcgat acccgcgata accgtcggca tgcgccggct gagcgaaccc
      901 tgccagcgcc aaaaggagtg cacaggcgaa gatgattcgc atggaaggcc cccctgcccc
      961 gatgacatat cttggcatag agcggcccga gcgtcagcct aagagttgtc ttttgccgct
     1021 gccttggcct cggcggcggc cttcgagaac cagcccgcat cggtgccgac ggcgacaacg
     1081 tcccacccgt ccttgacgag cggcttggca tagtcgacgt cgttggcgaa aatcaaagcg
     1141 attacagacc tctcccggca tttgctcagc accagcgtca tcgcgtcctt gacctcggcc
     1201 gcccagatgt cgagccgccc ggtgagcgcc accgaaaggt cgttcgggcc gaaacagacc
     1261 atgtcgacgc cgggaagcgc gaggatctcg tcgagctgat cgagcgcgcc cctggtctcg
     1321 atctgagggc aggcgatggt ccagtcattg gcgatcttga aatagtcggc gcccgggtga
     1381 tcgagccgcg cccggtaagg tccccagctc cgattgcccc gcggcggaaa tttcaccgcc
     1441 tgcacgaagg cctccgcgtc ctcaacattg ccgatcaagg ggcacatgat gccttgagcg
     1501 ccggcgtcga gcgcgcggcc gatgagcccg ggactgtttt cggcgacccg gacgatcgcc
     1561 ggcagcccgc aggcgcgcgc cgccgtaagg catccgacca gggcctcgtt gccgcccagg
     1621 ccatgctgct gatcgatggt gatgcagtcc cagcccgcct cgccaatcag ctcgatgagg
     1681 aaggggttgt tcagcgtaag ccaggcgttg aacagggtgc ggcgttcctt tgccgccctg
     1741 cggaagcggt cttcgcgcat cggcgcgttc aacccttcga ttcggattcg atctgcgcga
     1801 tggcttcgcc catcagctcc gtcatcatgc ggcgaatctg atggtcggac tggccgaccc
     1861 ccttggcatc gaagtcggcc ctgatcttgc gaaagacgtc ctcgtcgccg ggttcggcga
     1921 gatcggcctt gacgacctcg cgcgcgtagg ccgtcgcatc gtcgcccgtg acccccatct
     1981 gcccggcggc ccacagcccg agaagccggt ttcgccgcgc cgtcgctctg aacttcagct
     2041 cctcgtcatg ggcgaacttg cgctcgaagc ccttcttgcg ttcctcaaaa gtggtcattt
     2101 ggccttcgtc tccttgagcc ctttcgattc gggggcaagc cctcggaaat cctgggggaa
     2161 cccgacccct cgcggggccg agggataacg cccaaaagcc cggaaatcaa cacgttgcgg
     2221 ggacacaccg catccgtccc ggcagtcatt gtatgccccc tacccttacg ctaaggtcgg
     2281 cataagaccc tttaaatcga ttccggcaat ggcgatctgc aagttgcgcg cggcctgggt
     2341 gaggctctcg cccgcgcccg tggcgcttgt cggcagtctg agccgagagt gagtgaggaa
     2401 cccattatga atcgccgccg gcgcgtctat gaaggcaagg ccaagatcct gtacgagggt
     2461 cccgaacccg gcacgctcgt tcagcatttc aaggacgacg ccaccgcctt caacaacaag
     2521 aagcacgaca agatcgaggg caagggtgtc ctcaacaacc gtatctccga atacctgttc
     2581 cagcggctcg gcgaaatcgg cgttccgacc cacttcatca agcggctcaa catgcgcgag
     2641 cagctgatcc gcgaggtcga aatcatcccg ctcgaagtgg tggtgcgcaa cgtcgccgcg
     2701 ggctcgctct cgacccggct cggcatggac gaagggacgg cgctgccgcg ctcgatcgtc
     2761 gagttctatt acaagaacga ccagctcggc gatccgatgg tcaccgagga gcacatcacc
     2821 gccttcggct gggcgacgcc acaggagatc gacgacgtca tgtcgctcgc gctccgcatc
     2881 aacgactttc tctcggggct gttcctcggc gtcggcatca ggctcgtcga tttcaaatgc
     2941 gagtttggcc ggcagtggga aaacgagcag atgcgcatcg tgctcgccga cgagatctcg
     3001 cccgattgct gccggttgtg ggacatcgag acgcagaaca agctcgacaa ggaccgcttc
     3061 cgccgcgaca tgggcggcct tgttgaggcc tatcaggagg tcgcgcgccg gctcgggctt
     3121 catgtcgaca atccgatgcg ggcgcgcacg ggcccggtgc tggtgcgctc gaagtaaggt
     3181 cgcagcgagg gggcatgaag gcgcgcatca ccatcacgct caagcccggg gtgctcgacc
     3241 ctcaaggcaa ggccatcgcc ggcgcgctcc atgcgctcgg gttcggctcg gttggcagtg
     3301 tgcgccaggg caaatatatc gaggtcgagg tggccgagag cgatccttcg cgcgcccgcg
     3361 ccgaggtcga acgcatgtgc gagcagcttc tcgccaacac catcatcgag aactacgcct
     3421 atgaactcga ggcggcttga ggcatccctc tcccctggcg ggagaggatg gcgaagccga
     3481 gcaagagcga ggctgcgccg ggtgaggagt agttttagac gcgcgactgc cttgccgatt
     3541 tgcaatgcaa gccccccacc cggcacgcaa gagcgtgccg acctccccac gagggggaga
     3601 tttggcgctc ggacgcatgg taaagccact gaaattcgca tgccactctc cccttggggg
     3661 agagcacacg agcgcgtttt cgcgcttgtg gtgaggggaa agccgcgaag cggcgtcgaa
     3721 gagaagaacc ccctcgcccg cctcgccgcg ctttcgctcc tgcggagcta cggcggcctc
     3781 ggcaccctct cccgcgaggg gagagggatt ttcttcggag ccgtttgata tgaaagcgag
     3841 cgtcatcgtc tttcccggat cgaattgcga ccgcgacgcc gccgtggcgc tcgaagccgc
     3901 caccgggcat gcgcccgcga tggtctggca cggcgattcc gacatcccgc caagcgacct
     3961 gatcgtgctg ccgggcggat tctcctacgg cgattacctc cgctcgggcg ccatggcggc
     4021 gcattccccc gtcatgcgcg aagtggcggc gcgcgcccgc gtcggaaccc ccgtgctcgg
     4081 catctgcaac ggatttcagg tcctgaccga ggccggattg ctccccggcg tgctgatgcg
     4141 gaacgccacg ctcaaattcg tctgccgcga cgtgttcctg cgcgtggagc gcaatgactc
     4201 gatcttcacc caccgttacg cgcaaggcga agtggttcgt ttccccatcg cccacaagga
     4261 cggcagctat ttcgccgacg acgcgacgct ggatcgtctc gaaggcgaag gcctcgtcgc
     4321 gttccgctat tgcgacgctg cgggggcaat taccgacccc gccaatccca acggctcgcg
     4381 ccgcaacatc gcgggcatct acaacgcgac cgggaccgtc ctcgggctga tgccgcaccc
     4441 tgagcggctc gccgaccccg cgctcaacgg caccgacggc gccaagatgt tcctctcgct
     4501 ggtggagacg ctccattgaa catcgcggcg cgcgcggcga aagcggccgg gagaatcacg
     4561 cctgagatcg ttgccgaaca cggcctcggt caggacgaat acaagctgat ggtcgcggcg
     4621 ctcggccgcg acccgtcgct gaccgagctc ggcatcttct ccgtgatgtg gtcggagcac
     4681 tgctcctaca aatcctccaa ggtctggctc aggaagctcc ccaccgaagg cccgcaggtg
     4741 atccaggggc cgggcgagaa cgcgggcgtg gtcgatctcg gcgacggcca ggcggcggtg
     4801 ttcaagatgg agagccacaa ccacccgtct tacatcgagc cctatcaggg cgcggcgacc
     4861 ggcgttggcg gcatcatgcg cgacgtgttc accatgggcg cgcgcccgat cgccaatatg
     4921 aacgtgctca gatttggcgc gcccgaccat cccaagacca agcaccttgt cgccggcgtg
     4981 gtctcgggca tcggcgacta cggcaattgc atgggcgtgc cgacggtcgg cggcgagacc
     5041 aatttcgatc cgcgctacaa cgacaacatc ctggtcaacg ccatgtgcgt cgggctcgcg
     5101 gacgcgggtc gcatcttcag gtcggcggcc aagggcatcg gccatcccgt cgtctatgtc
     5161 ggctcgaaga cgggccgcga cggcattcac ggcgcgacca tggcttcggc cgagttcgac
     5221 gacgcgtctg aggagaagcg cccgaccgtg caggtgggcg accccttcac cgagaagctc
     5281 ctgctcgagg cgtgcctgga gctgatggcc gaggacgcca tcgtcgccat tcaggacatg
     5341 ggcgccgcgg gcctgacctc ctcgtcggtc gagatggccg acaaaggcgg cgtcggcatc
     5401 gagctcgaac tcgaccgcgt gccgacgcgc gaggacggca tgaccgccta cgagatgatg
     5461 ctctccgaga gccaagagcg catgctgatg atcctcaagc cgggctcgga gaagatcgcc
     5521 gagcgcgtgt tccgcaaatg ggagctcgac ttctcggtga tcggctacac caccgacact
     5581 gggcatctcg tggtgcgcca tcgtggcgcc gttgaggccg acgtccctct tgcggcgctc
     5641 gcccatgccg cgcccgtcta cgagcggccg tggaccaaga cggagccgca agagaccgtt
     5701 gtgcccgagg cggtgccggc cccgaacagc cagcttggcg cactcgagtg cttgatgggg
     5761 tctcccgcgc tctgctcccg ccgctggatc tggcagcaat acgaccacat ggtgatggcc
     5821 gacacggtgc agagaccggg cggcgacgcc gcggtggtgc gcgtgcacgg cacgaggaag
     5881 gggctggcca tctcttgcga cgtgaccccg cgttattgcg ccgccgatcc gttcgagggc
     5941 gccaagcaag cggtcgccga atgctggcgc aacctcaccg ccgtcggcgc cgatccgctg
     6001 gccatcaccg actgcatgaa tttcggcaat ccggagcggc ccgagatcat gggcgagttc
     6061 gcgggcgcga tcgaaggcat ggctgaggcc tgcacggcac ttgccttccc cgtcgtttcg
     6121 gggaacgtgt ccctgtacaa cgagaccaac ggcgtcgcca ttccgccgac accgtccatc
     6181 ggcggcatcg gcctcatccc cgacatcgcc acgatggcga ccatcgcgct gaagcgcccg
     6241 ggcgacgtgc tgatgctgct cggggccgag gccggccatc tcggccagtc gctctatatg
     6301 caggtgatgt tgaaccggac cttcggcgcg ccgccgccgg tcgacctcga cgccgagcgc
     6361 cgaaccggcg acctggtgag agggctgatc agaagcgggg cgttatcggc ggcgcacgac
     6421 gtgtcggatg gagggctgct ggtcgcgatc gccgagatgg ctcttgccgg gggtctcggc
     6481 gtcgcgctcg acgcgacccc cgctccgctg ccgccgcatg ccacctggtt cggcgaggac
     6541 caggcccgct atgtcatcgc cgcgtctccc gacaaggccg atgccatcca caaggcggcg
     6601 gccgcggcca aagtcccgct caggctgctc ggcagcgtgg gcggcgacgc gatcacgctc
     6661 gacggcgagt ccgcgctgcc cttggccgcg ctctgggccg cgcatgagag ctggttgccg
     6721 cgcttcatgg cgcacggcta ggatcgcagc agacaaggag caaagcgatg cccatggccg
     6781 ccgacgacat cgcgcgcctg atcaaggagc gcttcccgga cgcggaggtc cgcatcgagg
     6841 acctcgcggg cgacggcgac cattacgcgg cgaccatcgt ctcggaggcg ttccgcggca
     6901 agacccgcgt ccagcagcat cagatggtct acgatgcgct gaaaggaaat atgggcgggg
     6961 cgctccacgc tcttgcactc aggacggcgg cgccctagct accgggagaa ccgggaaagg
     7021 atcgacgatg agtgctcaag acgtccagga ctggctccgg aaagagatcg cccgcagcga
     7081 cgtggtgctg ttcatgaagg gcagcaagtc catgccgcaa tgcggcttct ccatgcaggt
     7141 cgcgcagatc ctcaaccatc tcggcgtcga tttcagagac gtcaacgtgc tcgaagacat
     7201 gggagtccgg gaagggatca aggcctattc gaactggccc accatccccc agctctatgt
     7261 caaaggcgag ttcattggcg gctgcgatat cgtgcgcgag atgtttcagg ccggcgagct
     7321 tcaggagctt ctgacctcca aaggcattgc tcataaccag cagacgatga cggcgtgatc
     7381 ggggcgcggg ccgcgtctac tcgcagccga tgatgatgtc gccggggttg atcatcagga
     7441 cgaccggctt gccctcgtag agcttcagcg tctccagtgc cgggatggtg acgctcactt
     7501 cgagcatgct gtcgccggga accgcgacat agacgatggc cgtcaccgcc cctttgcgga
     7561 tctccaggat cttgcccttg atctggttgc ccgtgctgaa tttcatgtcg gccatgcgtt
     7621 cctcccggct cagctcagat tgccgcaaaa gcgctgaatg cggcggcagg cttcttccag
     7681 ctctttgatc cccgtcgcat aggagatgcg gaagaagggc gagagcccga acgctgcgcc
     7741 atgcacgacg gcgacgcctt cctcttccaa cagcgcggtg gcgaaatcct cgtcattgcc
     7801 cacggtcttc ccgccgcgcg tggtcttgcc gatggttccg gcgcatgacg gatagacgta
     7861 gaacgcgccc tccggcttgg gacaggacaa tcccttggcc tggttcaaca tggcgacgac
     7921 gaggtcgcgc cgctcctgga acgaggcgac ccagtctttg agaaaattct gcggaccctc
     7981 gagcgcttcg accgcggccc attggctgat cgagctggga ttggaagtcg attgcgattg
     8041 cagcgtggcg atggccttga tcaaaggctc cggcccgccg ccatagccga tgcgccagcc
     8101 ggtcatcgaa taggcctttg acacgccgtt gagcgtcagc gtgcggttct tgagtcttgg
     8161 ctcgaccgcc gcgggcgtcg tgaacttgaa cccgtcatag acgagatgct cgtagatatc
     8221 gtcggagagc acccacacct tctcgtgctt gaccagcacg ccggtgagcg ccttgacctc
     8281 gggctcggta taggcggcgc ccgtcgggtt cgacggcgag ttgaagatga accacttggt
     8341 ccgcggcgtg atggcgcgca ccaacgcctc cggcttcaac ttgaagccgt cttcgagctt
     8401 ggtctcgacg acgatcggct cggccccggc aagcagaacg atgtcgggat agctcaccca
     8461 atagggcgag ggcacgatca cctcgtcgcc cggatcgagc gtggcgagca gcgcgttgta
     8521 gagcacctgc ttcccgccgc tgccgacggt gatttccgac ggcttatagg cgagcccgtt
     8581 ctcgcgtttg aacttctcgg caaccgcccg cttcagttcg ggaatgccgt cgacggcggt
     8641 gtatttcgtc tcgccgcgct tgatggcctc gatggccgcc tgcttgatgt tgtcgggcgt
     8701 gtcgaaatcc ggctcgccgg caccgaggcc gatcacgtcg aaacccgcag ccttcagctc
     8761 gcgcgccttg ctggtgatcg ccagggtcgg cgaaggtttg acccgttcga gcgtgcgggc
     8821 gaccagtccc atatcgatct cctggaaaat gcgaaaaacg aggcggccgg agcgggcgca
     8881 ccctaagcgg gggcgcgatt gcccgcaagc ctcgaccctc tttcgcgcgt cctgtgcata
     8941 accgcatttg cgccacagtt atggcaggga agcgcgcggg cgagccacaa tctggttaaa
     9001 gcaccgccta attagcatgg ttgattccgc ttggtgccga cagacgaggc tccagtcgat
     9061 gaccaagcga cgccaagctc tgtgcgcagg caagcccgcg gcaacttcgt tattccttgc
     9121 cctgatcccg atcgttctgt tcacgacgcc gagcgtcttg gcgccgagcg tcttggctgt
     9181 tgcgccagcc tcatccggcg ccggcctgct catgctgatg ggtgcattca cgctggtctt
     9241 ttggcattgc caccgccgcg acgatacctc cccgcggcga gtcgggagga ggatctgatg
     9301 ccgctacggg ggtcggcgca gctcctcctc ctctttctcc gcgcgcacag caaacgcggc
     9361 tggatcatgg gagcaattgc cggcggcgcg gtcgcggccg gcgtgtccct tggcgcaggc
     9421 gccgttccgc ttccgacagc ggccgagctg gcgcaggcgt tacgggagag cgcctgggac
     9481 cgggcgctcg cggaagcgcc ggcaacgtcg ccttggcctt gggcggatgt gcccgccgcc
     9541 acaacgggaa aggtcgcccg tcttggcctc agcgcagcaa tggtcaagga aggccggagc
     9601 cacagcatgc gcgccgtcga cgagcctcta cggccggctg ccgccgcggc cccgcagaaa
     9661 tccggcacga tgctgagcaa gcttgctgtc ggagaccgca tcacggtcac caccgccagc
     9721 ggagattcgc gcgtctatcg ggtgactggc ccgaaggtgg tcgatccgca tttggcggag
     9781 ggcggttctc aagccgtgag cggcgatgcc tgcgtgccgc tcgacccggt gcttgcgagc
     9841 tcgcttgggc tcgtcattca ggcgatcaca gccgatcccc cggcgcccga gccggaacac
     9901 gagcagaagc tctaaagatt tcgcttcagc cttattgctg ctgccgccgc ttctttttcc
     9961 tcggggctgt ggaggtcgca tccgccgcgg cgggcggcgt gctcggctgt gcggcgggcg
    10021 ccgtaccggc ctgcggcgtc gcagaggctc cgggcgcggc ggtcccgggt tgtggcgctg
    10081 cttgacccgg cggcagagcc gcgccttgag gcgcgggcgc gcccggtagc gccgccgccc
    10141 caggaagggc tgcggcgggc gtctggggct gagtggagac gctcgggctc agtgtcggca
    10201 tcgtcgggcg ccgctccgcc tcgatcttct ccctgagccg atcgaggccc gcgcgatact
    10261 cggcgccgac gaagccgtcg agcatcaaag ccttccaacg cttgagcggg tttgagcccg
    10321 actcgtagag aaaggtccag gtgactttgc tgccggaacc cgatggaacg atgtcgtagg
    10381 cgctcgtacc ttccaagccg ttgtagtcca cggcaaggtc gatgtgacgg ttgggctggc
    10441 tctcgctgat ctccatgctg ccggtcccga tcgaaggttt cttgctcgtc catgtgaccc
    10501 gcgcgccctg gccggtctgt ggcccgccat agctcaccgc caattgcgga tcgcgcacgc
    10561 tccagggcga ccattcgccg aattcgtgga gattgttgag atagggaaag atcgccgatt
    10621 ccggcgcgtt gatgaccacc gatcgcgcca cggtgacatg cgatggcaaa ccgagcgcga
    10681 cggcggcaaa aatgccggcg agcgcagcgc tccccaggac caaccgcgtc attggacgca
    10741 ttgcttcgcc tccttaattt cccgcaccca tgacggccgc tcgccccagc ccgtacgtcg
    10801 cgcccgacag agtaaccccc tcgcttttgc aaagaaaacg attacagtcc ggtcgtcaaa
    10861 caaaaggggg gagagatgac tctatcagca ccgtcgacgg tggtctttat cgtttcggcg
    10921 attttgtggg ctttggccgt aatcggcaat ttctcgcaaa ttcccttcgt cactgagaac
    10981 gggttctggg tcgcggtcat cgcctacttg atcctcgcgg tcggcaattt gttcagaggc
    11041 ctctagcgtt cggggtacgt cccagggcga gtgtgcccgc ccaccgcctc ctcaagttgc
    11101 cgcgaaccgg atcgagtctc gtgccgggcg catcagcccc gggggcctga tcgcgaaaca
    11161 caggcgcgac ccggcgcgga agcacgcagc cgattatcgt ctgccgtgtt gggcgagcga
    11221 gaggggagag ccggcggcct cggcgggccc tcccctgatc gctctacatg tcgtcgtcgt
    11281 cgcgcgccca ggacggccga taaggctgcg ggctcgggct cgatggcgtc ggcgttgccg
    11341 ggcgcggcgc cccgcccggt gcgggcgatc ctccgcccat ggggctcgcc ggtcgcggcg
    11401 gcatgccgct actgcccatg ccgggcgacg gtgccggccg gggtggcgcg gccgacggct
    11461 gcgccgcagg ccgcgagact gtcggcgcgg gcgctgccgg ctttggcgct gcgggtttcg
    11521 cggccgcggg cttacgggcc ggggctttgc gcgcgggctt ccgcgccgtc ttctttgcgg
    11581 tttttcgcgc ggcgctcttc cgagccgggg ccttccttgc agcggctcgc ttcgccgttt
    11641 ttttcttggc gcctgtcttc cgtttcgcgg cggtcttgcg tgcgctcgtt ttcctagccc
    11701 ccgcgctttt cttcctactc gttgtgcgct tcttcccact ctttttcgta gccatggctt
    11761 gtgctccgat cctgctctga tgccgcactc gaccgctcaa acggacgcgt acctgcacga
    11821 ctcacgcttc accttgccca ggcttgccgt cccgcgagac gagtcaaatc atttttttca
    11881 ttatacgatg atcgccgccg aaagtgacaa tttgggttca gtcatccgca acaacctgaa
    11941 cgcgccaatc gccccagccg tgcatggttg agggcaaaaa agcgggtttc cgcagggagc
    12001 tgctctcgcg aaatccgcgc ggaattcccg tctcttcgcc cacctcggcc actggcgagg
    12061 gcatgaagga agggcttcca tacccgtccg gtggcgaaga gtcgggtcga caagtcgcgc
    12121 tttgacccgg ccgtccttgc cgatcacgag ggtcgccggg atgggcaaga gccacccccg
    12181 tttccctgaa acgctccaga tcgatgccga acttcaaggt ggtgcctcaa ggcggaggcc
    12241 aaaccgacgg catttcgcgc aaggcgtgtt cgaaggggag cgaggagacc tggacggcgc
    12301 cggtgaaggg atagtcgaag acaatggcca gcagcagggc aatcgcgacc agcacggcca
    12361 atgacgcggt catgagaacc tgcgccccga cattggtcga ggcgaagaaa gcgggaaagc
    12421 cgagcgtgat caatccgccg acgatcagca cgatccacag gatccccggc accgagccgc
    12481 ttgcgctgtc gagccgttcg ttgcggttat cggtcatgac cgccagaagt ctcagcgcgt
    12541 cttgatatag ggccaactcc tgccacgttg cgggattgac ggtgaagatc gccttcgtca
    12601 gccgatcgag gtccttgacc acgacgtcgc tcggctcgcc cacggccatc gcgggccatt
    12661 cgtgttcgcg cacgtccttg gtgtaggcga tgagatgctt gcggatttcg tcgcctgctt
    12721 cggccggaag ggcgaaggtg atgcggtgca agtctacggc cgccttcgcc tcgtcgcgga
    12781 ctgtcgcctc ggtctggcga aagtcctccc acacggccac gaccacgaag gcgagcagca
    12841 cggcgtaaaa gacccctacg actgcgaatt tgaatcccgc gacctcgttg ttcaggctga
    12901 gccggtccac gccgtagcgg aaccgcgtcc cgagcatgag gccaatggcg taaccgcagg
    12961 cgaccaagac gagcagcaag gtcaagagcc acagcggaac gtcgttggag aggtaaagca
    13021 ggttcatccg tgagactatc ccgtccgcgg ttcgtagccc ctttctaccc gaaaccggtt
    13081 tacaagagtc ttgtcgcgca ccgttccctt ttcccacgcg accgccgagc aacctccccc
    13141 tcgtggggag gtcggcgcgc tcttgcgcgc cgggtggggg gctggctcta atcccaccca
    13201 gctcgtccgc tgagacttct caccgcgctc gcggtgcgag cacgcgctct ccccaagggg
    13261 agagcagccg aaaacgtcga ttcaagttgc gcgaccgccc caaatgcgcc gaagcgaagc
    13321 cgtcccttgg tggtgcttcc gtgcatcagg agctcaggca ggttcgctgc tctagcgcca
    13381 ggcggcgttg gggtgggggc tcgcgctgca actcacgacc ggtttaccat ttggaatctc
    13441 aaccccccac ccggcacgca atgcgtgccg acctccccac gagggggagg tttgaaatga
    13501 cgcattgccg aggtcggagt cggcgctcct catcaagggg agggtggctt cgcgcaaggg
    13561 gtagccaagc cccctccccg ccgccatatt cggcccatgc cccatcgcat cgccaggacc
    13621 ttcggctctc gcgccgagcc gcgcgccgtc acgggtgaat cggccgggct gccgctcggc
    13681 gccgaccgcc gtcctccggg tttcggcgaa acaccgcagc ctctgat
//
LOCUS       DASUQP010000112         5503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1686710, whole genome shotgun sequence.
ACCESSION   DASUQP010000112 DASUQP010000000
VERSION     DASUQP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686710"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFY74_05000"
     CDS             complement(<1..859)
                     /locus_tag="VFY74_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX5931095.1"
                     /translation="MDTLLRISQKKQDEEIRRSLKLGLEAADSVIDRNISLFSRGHQP
                     AFAGINTFMKAPYCEDIRKVGDYEAAFVGAPFDSATTYRPGTRFGPQAVRRISALYDG
                     YWFDGGVDIFEELDLCDAGDIFVIPGNIEKTFDQVTKAVSHLYTSGVFPIICGGDHSL
                     GFPNVRGIAPHIDGNVGIIHIDRHIDIQEKDMDERMHTTPWFWTTHEGTVTTHRDHSH
                     MHDVGLPNCHPKNLVQVGIGGWYGNRPGTAVAKRRGTSVLTMKDIEVHGARKAAEIAL
                     DMAWEGCKAV"
     gene            1315..2169
                     /locus_tag="VFY74_05005"
     CDS             1315..2169
                     /locus_tag="VFY74_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5931096.1"
                     /translation="MISALFGIASAFTLGAADFMARFSTRALGAVLTYTFVLLVGAIG
                     ATIWFFASGQVLVWSPLGVAIAVAHGISVAAMCVLLYIGIARGPIAVVAPIVAAHPAL
                     VLAVNVLMGVRPSAVQWAAMIVVIAGGVLIARSAVSEGETSDAAANRTTLLIAFSACL
                     AYVAIVLTAQAATPLIGAFQTMWIGRLAGLALLGAVLLVQQARITVPREWLPFMALQG
                     GLDTMGYLTFLAGSNSAAPYVTMVLASAFSVVTVLLARLILHEPISKLQWAAISLIAV
                     GAAVLSVS"
     gene            complement(2173..2802)
                     /locus_tag="VFY74_05010"
     CDS             complement(2173..2802)
                     /locus_tag="VFY74_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5931097.1"
                     /translation="MKAFVLSSSPRRNGNSALLAEAIREGLAEAGHEATLLFADDFLK
                     CFLRDCRKCRKPDGECSIEDGYRSVFFDHYLPAEGFIAATPIYWYGTSAQLKAFFDRT
                     FCYYAASYPKSAEVIEKMQGKRIGLALSSEETFPMVSGGIISQVQEFVRYTHCTFVGV
                     VHGYGNARGDVLKDPCQPIEAARRFGREFFTRHTSDYQIDSVRSGRVWG"
     gene            complement(2806..3816)
                     /locus_tag="VFY74_05015"
     CDS             complement(2806..3816)
                     /locus_tag="VFY74_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5931098.1"
                     /translation="MQGYDTYALGDVRLQSGQVLKDAQLAYATYGTLNSGADNAVLLP
                     TFYTGTHIRNEPLFGPGRAIDPARHFIVSVNLFGNGYSSSPSNAPDAQAGPRFPHVTL
                     YDNVTCQHRLLTERLGVRRIALVLGWSMAAMQAYQWGAQYPDMVEAILPYCGAARCSP
                     LNYAFLDSPKAALQADAAWNDGNYVKPPEKGLRAFGRVYVAWAYSHAFFRDGLYRTLG
                     FATLEDFVRDWEEDHLKWDANDLIAKIWTWQHGDIGDNEIYRGDFSLALQSITARAIV
                     MPCSTDMYFVPADNAAEVRKMQNAELRVFESPWGHCVASPGRVPEFQRSLDAAAAELL
                     RG"
     gene            complement(3857..5110)
                     /locus_tag="VFY74_05020"
     CDS             complement(3857..5110)
                     /locus_tag="VFY74_05020"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX5931099.1"
                     /translation="MAHAADITEANRLAFERLTKSDPVLADVRRVGDFVAGFTPNTIL
                     TSGPPMAWQDYTGGQREAIIGGALFEGLAKDREDAIAKLDAGEIVVGGCHDFGCVGSL
                     AGIYTASMPAFVVENRAFGNVACCNIYEGSNPRRLNYGVYDDGVRERLLLVQNEIVPV
                     IAEALRQTDGIPLKPIIKRALGMGDELHSRNTAAALLFLRELVPNLLTLASSGSERIV
                     RAVKAMTDDHYFFLRLSMAAAKATADAARNIERSSVVTALAFNCRGFALRVSGLGDTW
                     FKGPHASVEARLFEGHTEDEITWMGGESIIAETIGLGGFAQASAPSLQSYQGGSYETM
                     LARNKELYAITVGENPDYLIPALGFRGTPTGIDIFKVIDQKVLPVLDIGIAGRNGGQI
                     GAGVVRPPLACFETAAEAYRQRYSS"
     gene            5296..>5503
                     /locus_tag="VFY74_05025"
     CDS             5296..>5503
                     /locus_tag="VFY74_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX5931100.1"
                     /translation="MTEQPIRDLARGRRAGEDARKRSFARYRPSPGRVDSFLVASLEL
                     FAERNFASVTIKDIAKALDVNPALI"
BASE COUNT          993 a         1695 c         1759 g         1056 t
ORIGIN      
        1 agaccgcctt gcatccctcc catgccatgt cgagggcgat ctccgccgct ttgcgcgctc
       61 cgtgcacctc gatatccttc atggtgagaa cgctcgtgcc gcggcgtttt gcgaccgccg
      121 ttccgggccg gttgccgtac cagccgccga tccccacctg caccagattt ttgggatggc
      181 agttcggcag tccaacgtca tgcatatgac tgtggtcgcg atgtgtggtg acggtcccct
      241 catgggtcgt ccagaaccac ggcgtcgtat gcatccgctc atccatatcc ttctcctgga
      301 tatcgatatg gcggtcgata tggataatgc cgacattgcc gtcgatatgc ggcgcgatcc
      361 cgcggacatt gggaaagccg aggctgtggt cgccgccgca gatgattggg aagacgcccg
      421 aggtgtagag gtgcgaaacg gccttggtca cctggtcgaa ggtcttttcg atattgccgg
      481 ggatcacgaa gatgtcgccg gcatcgcaca ggtccagctc ctcgaaaatg tcgacgccgc
      541 cgtcgaacca atatccgtcg tagagcgcgg agatgcgccg cacggcctgc ggtccgaacc
      601 gcgtccccgg tcgataggtc gtcgccgaat cgaagggggc gccgacgaag gccgcttcat
      661 aatcgccgac cttgcggatg tcctcgcagt agggcgcctt catgaaggta ttgataccgg
      721 cgaaggcggg ctggtggccg cggctgaaca gggagatgtt gcgatcgatg accgagtctg
      781 cggcctcgag cccaagtttc agcgagcgcc ggatttcctc gtcttgtttc ttctgcgaga
      841 tgcgcaacag cgtgtcgatc gtcttggcgc cgtgggttgc gctccgcttc acgccatgcc
      901 tatgtctccg actatgcggc tgaccgcgac ttccgccctt tttgcctcct gccatgcgaa
      961 actcctaaac accgtacatt tgacgctctc gctcggggcg atcccaccaa atcagacccg
     1021 atccttcaat agccattatt gatcaactga tgctgcaccg ccagaagcgg ccctaagtcg
     1081 cgcgcgcctg gccaatggag tttgacggga aatgagaagc ggaaggaagc atggcggggc
     1141 acctctgatt gccggttcgg gggcgactga cggacagacg tgcaattcaa ctatgacttg
     1201 gcatcctttg caaaagcaca gtgagcgaca cgctcggctt gaaggggtcc ccccgcgcaa
     1261 gctgtcggcg aatctgacgc gccctgtttc caacgccacg cgaaagagcg ccgcatgatc
     1321 tcggcgcttt tcggtatcgc gagcgccttc accctcggtg ccgccgactt catggcgcgg
     1381 ttcagcacgc gcgcgctggg cgccgttttg acctacacgt tcgtcctgtt ggtcggggcg
     1441 atcggggcga cgatctggtt tttcgcaagc ggccaggtgc tcgtttggtc gccgctcggc
     1501 gtggcgatcg cggttgccca tggtatcagc gtggccgcga tgtgcgtgct gctctatatc
     1561 ggcatcgcgc gcgggccgat tgccgtggtc gcgcccatcg tcgccgcgca tccggcgctg
     1621 gtgcttgccg tgaacgttct catgggcgtg cgcccgagcg ccgtgcaatg ggcggcaatg
     1681 atcgtcgtca ttgccggagg cgttctcatc gcacggagcg ccgtgtcgga aggcgagacc
     1741 tccgatgccg cggccaaccg gacgacgctg ctcatcgcct ttagcgcgtg ccttgcctac
     1801 gtcgcgatcg tgctcaccgc gcaggccgcg acaccgctga tcggcgcgtt tcagaccatg
     1861 tggatcggcc gcctcgccgg cctcgccctc ctcggcgccg tcctcctcgt gcagcaagcg
     1921 cggatcacgg ttccgcggga gtggttgcct ttcatggcct tgcaaggcgg gctcgatacg
     1981 atgggctatt taacgttcct ggccggatcg aacagcgccg cgccctatgt caccatggtg
     2041 cttgcctcgg ccttcagcgt ggtgacggtc ctgctcgcgc ggctgatcct tcacgagccg
     2101 atcagcaaac tgcaatgggc cgcgatttca ctgatcgccg tcggtgccgc ggtcctgagc
     2161 gtgagctagg cgctatcccc agacccgtcc ggagcgcacc gagtcgatct ggtaatcgct
     2221 cgtgtggcgg gtgaagaact cgcggccgaa gcggcgtgcc gcctcgatcg gctggcacgg
     2281 gtctttcagg acatcgccgc gcgcgttgcc gtagccgtgc acgacgccga cgaaggtgca
     2341 gtgcgtgtag cgaacgaatt cctggacctg actgatgatc ccgcccgaga ccatgggaaa
     2401 cgtttcttcc gagctgagtg caagaccgat gcgctttcct tgcatcttct cgatgacttc
     2461 ggccgatttc ggataggacg cggcgtaata gcaaaaggtg cggtcgaaga aggctttgag
     2521 ctgcgccgat gtgccgtacc agtagatcgg ggttgccgcg atgaagcctt cggccggcag
     2581 atagtgatcg aaaaagaccg agcgatagcc gtcttcgatc gagcactcgc cgtccggctt
     2641 tcggcacttc cggcagtccc tgagaaagca tttcaggaaa tcgtcggcga aaaggagcgt
     2701 cgcctcgtga ccggcctcgg ccagcccctc gcgaatggcc tcggcgagaa gcgctgaatt
     2761 gccgttacgg cgcgggctcg atgacagcac aaatgccttc atatctcatc ctctcaacaa
     2821 ttcggcggcg gccgcgtcaa gagaccgctg gaactcaggc acgcgtcccg gcgaagcgac
     2881 gcaatggccc caaggcgact cgaaaacgcg caattcggca ttctgcatct tgcggacttc
     2941 ggccgcattg tccgccggca cgaaatacat gtcggtgctg cacggcatga cgatggcgcg
     3001 ggcggtgatc gactgcaatg cgagactgaa gtcgccgcgg taaatctcgt tgtcgccgat
     3061 atcgccgtgc tgccatgtcc agatcttggc gatcaggtcg ttcgcgtccc acttcaaatg
     3121 gtcctcctcc cagtcgcgca cgaagtcctc gagcgtggcg aagccgagcg tacggtagag
     3181 gccgtcgcgg aagaacgcgt gcgaataggc ccaggcgaca tagacgcggc cgaaggctct
     3241 caagcccttc tctggcggtt tgacgtaatt gccgtcgttc caggcagcgt cggcctgcaa
     3301 ggcagccttc ggactgtcga ggaaggcata gttcagtggc gagcaacgcg cggcaccgca
     3361 atagggcagg atcgcttcga ccatgtccgg atattgcgcg ccccattgat aggcctgcat
     3421 cgccgccatc gaccagccga gcacgagcgc gatacgccgg acgccgaggc gctccgtgag
     3481 caggcgatgc tggcaggtga cattgtcgta gagcgtgacg tgcgggaatc gaggaccggc
     3541 ctgcgcgtcg ggcgcattgc tcggcgacga ggaatagccg ttcccgaaca gattgaccga
     3601 aacgatgaag tggcgggcgg ggtcgatggc gcggcccggt ccgaacaagg gctcgttgcg
     3661 gatatgcgtg ccggtataga aggtgggaag gaggaccgcg ttgtccgccc cagagttgag
     3721 cgttccatag gtcgcgtagg cgagctgcgc gtctttcagc acttgacccg actggaggcg
     3781 aacgtcgcca agcgcgtaag tgtcgtagcc ttgcatcggc ggctcgtctc gaggtcctgc
     3841 tgctagagcc gaaatgtcat gagctatagc gctgacggta ggcctccgca gccgtctcga
     3901 aacatgcaag cgggggccgc acgacgcccg cgccgatctg tccgccgttc cggccggcaa
     3961 taccgatatc gaggacggga agcacctttt ggtcgatgac cttgaagata tcgatccccg
     4021 tcggtgtgcc gcgaaacccg agcgccggaa tgaggtagtc gggattctcc ccgacggtaa
     4081 tggcgtagag ttccttgttg cgcgcgagca tcgtctcata ggagccgccc tgatagcttt
     4141 gcagagaggg ggccgaagcc tgggcgaagc cgccgagccc gatcgtttcg gcgatgatgc
     4201 tctcgccccc catccaggta atctcgtcct ccgtatggcc ctcgaacagt ctcgcctcga
     4261 ccgaggcgtg gggtcccttg aaccaggtgt cgccaagccc gctcacgcgc aaggcaaagc
     4321 cgcggcaatt gaaggccaag gccgtcacga cgctcgagcg ctcgatattg cgcgcagcgt
     4381 ccgcggtcgc cttggccgcg gccatggaaa ggcgcaagaa gaagtagtgg tcgtcggtca
     4441 tggccttgac ggcgcggacg attctctcgc tccccgacga cgccagcgta agcaagttcg
     4501 gcacgagctc gcgcaggaac agaagcgccg cggctgtatt gcgggaatgc agctcatcgc
     4561 ccatgccgag cgcccgcttg atgatgggct tgaggggaat gccgtcggtt tgccgcaagg
     4621 cctcggcaat cacaggcacg atctcgttct gcacgagcag cagccgctcc ctgacgccgt
     4681 cgtcatagac gccgtagttc aagcggcgcg ggttgctgcc ctcgtaaatg ttgcagcagg
     4741 cgacgttgcc gaaggcgcgg ttttcgacca cgaaggccgg catggaagcg gtgtagattc
     4801 cggccaagga gcccacgcag ccgaaatcgt ggcagccgcc gacgacaatc tcgccggcat
     4861 cgagcttcgc gatcgcgtcc tcacggtcct tggcaagccc ctcgaacagg gcgccgccaa
     4921 tgatcgcttc gcgctgtccg ccggtataat cctgccaggc catcggcgga cccgaggtga
     4981 gaatggtatt gggggtgaag ccggcgacga aatcgccgac ccggcgcaca tcggcgagga
     5041 ccgggtcgga tttggtaagg cgttcgaacg cgagccgatt ggcctcggta atatccgccg
     5101 cgtgtgccat attcgcgcct cccagcgcta attagtcgtt taatgaggcg atcaagtgtt
     5161 gccttacgga ccgacctaat gcaagtagat gctatagtgc gatgaggcca tgccctcctg
     5221 cggcgagcgg atgatgcaag atttcgcttg cgtgcgcgtt catcggggca gccagcggcg
     5281 aaagctgagc aagagatgac tgagcagcca atccgggatc tcgcgcgcgg aaggcgcgcg
     5341 ggggaagatg cgcgcaagag aagctttgcg cgctatcggc caagccccgg ccgtgtcgac
     5401 agctttcttg tcgcctcgct cgagctcttt gccgagcgca acttcgcctc ggtcacgatc
     5461 aaggatattg ccaaagctct cgacgtcaat ccggcgctaa ttt
//
LOCUS       DASUQP010000113        12143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1551029, whole genome shotgun sequence.
ACCESSION   DASUQP010000113 DASUQP010000000
VERSION     DASUQP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551029"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFY74_05030"
     CDS             <1..345
                     /locus_tag="VFY74_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="H4MPT-linked C1 transfer pathway protein"
                     /protein_id="HEX5931101.1"
                     /translation="SASRLARMLGRDLSAGDLLAYVDVARHFADHQRAEIEAAALALE
                     AREGLPADAPVIGAGCGRFIAQHMAARLGRPYCDFAELFDCAPQARDMAGTCAPAVAV
                     GLLAAHELILDG"
     gene            complement(394..642)
                     /locus_tag="VFY74_05035"
     CDS             complement(394..642)
                     /locus_tag="VFY74_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5931102.1"
                     /translation="MGIIAWIVLGLIAGFIASKIYVGTGQGLLMDIVLGIVGAVVGGF
                     LFTALGATGVTGFNIWSMIVATIGAIIVLWLYHTLATR"
     gene            complement(687..1163)
                     /locus_tag="VFY74_05040"
     CDS             complement(687..1163)
                     /locus_tag="VFY74_05040"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEX5931103.1"
                     /translation="MVQNIFGSDRGPAVPGGSVAKPLVIALLALLASRYFGGKKKEEP
                     EADAPRLPEEAPQSTDASPGSIVDGLGGLIKQFQQKGLGDVVDSWVSTGKNKNVGSDQ
                     ISAALGGDVVDELSRRTGLTRDQVVAELARMLPNVVDNLTPDGRLPSRQEVMRLMS"
     gene            1317..1706
                     /locus_tag="VFY74_05045"
     CDS             1317..1706
                     /locus_tag="VFY74_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931104.1"
                     /translation="MKPWMLRVLTFAAVTACIGVGPSYEAAALPSPHVLNAVVAPTLV
                     EEGGWRRQWRRQGPGPIGVVPDTGVVVDPDVEIESEVDVEVDVEAGPPAVIFLPPPRP
                     ASCGQYRYWNGERCVDARYNDPYLGAR"
     gene            complement(1864..2442)
                     /locus_tag="VFY74_05050"
     CDS             complement(1864..2442)
                     /locus_tag="VFY74_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HEX5931105.1"
                     /translation="MPMIRECIVTTLSEEGKVHIAPLGLIAEEGGWIIAPFRPSTTLD
                     NLGVTPYAVANFTDDVLVFAGCLVGRRDWPTRPSTHVPGAVLEGVLAHAELAVTEVEA
                     DEQRPRFHCRVVHEASHAPFKGLNRAQGAVIEAAILASRLHMLPREKIERELGYLQIA
                     VEKTAGPREHEAWRLLVEKIEDHYKHAPPPPG"
     gene            complement(2481..3962)
                     /locus_tag="VFY74_05055"
     CDS             complement(2481..3962)
                     /locus_tag="VFY74_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HEX5931106.1"
                     /translation="MAESILFLTGHLARARLEAVLEAMTASFDWKVVDIGVKVAALMT
                     EDIITRRLQNPVAADKVMLPGRCRADLERLGERFGVPFLRGPDELKDIPLYFGRARKA
                     SDMSKYDIQIFAEIVDASALAVETILERARDYADAGANVIDLGCLPDTPFPHLEDAIS
                     ALRRQGYKVSVDSADAGELRRGGKAGADYLLSLNESTLAIADEVASIPVLIPKEHGDM
                     ASLYRAMDALDAKGRAYLVDPVLDPINFGFMQSLERYAQVRRERPDAEMLMGTGNLTE
                     LTDADTTGITAVLLGIASELHIKNVLVVQVSPHTRRTVEEHDLARRIMYASRTEGDLP
                     KDYADGLLTVHARRPFPQTPDEIAAAAREVRDKNYRIEIAEDGIHIYNRDGHHVAQDA
                     FDLFPKLSVENDGAHAFYLGAELVKAEIAFRLAKRYAQDEPLDWGVAADAPAEDKTEL
                     KAAGHTLVAKTGARYQVSGVRKTGAGYQVSGAKKGNDTDPTDT"
     gene            complement(4203..4880)
                     /locus_tag="VFY74_05060"
     CDS             complement(4203..4880)
                     /locus_tag="VFY74_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931107.1"
                     /translation="MARPLPRQLAQREPVAGEAALVVVKLGGSVVRSGELAAWLDAIA
                     AAPCPVVVVPGGGALADEVRSVQAALGFGDRAAHRMALLAMDQLAWAVAGLRQGFVVG
                     TTEPELRAALERGDVAVWAPYGLIAGRSDIEESWRLTSDSLALWLAARIGASRCFLVK
                     SVRRQGTSADAQELAQAGVVDAAFPSILKDARVPAVLLGRGDQQAFAAALAAKGTGRS
                     DFGAAIT"
     gene            complement(4884..5297)
                     /locus_tag="VFY74_05065"
     CDS             complement(4884..5297)
                     /locus_tag="VFY74_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5931108.1"
                     /translation="MAEPKLRQVAPNAAPDEAPALDRVFIRDLVLDVEIGVHNYEKGA
                     TQRVRFSIDVDLTRATRALDDDIARTFDYDTIIAGIKGIVARGHINLVETLAEEIARH
                     CLAHPRAARVAVRIEKLDKEPGAVGVEIVRAKGYV"
     gene            complement(5340..6071)
                     /locus_tag="VFY74_05070"
     CDS             complement(5340..6071)
                     /locus_tag="VFY74_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HEX5931109.1"
                     /translation="MTLFLASVRDAGEAEMAIGAGADIVDLKDPGAGALGALAPATVD
                     ACVRAIGGRVPVSATIGDVPLEQEGVRAAVLSMAARGVDYVKLGLFPGGDGERCLTML
                     ASLTPQIRLILVLFADALSEIDAIRLAARIGASGVMLDTFGKSGVSLPDLVSAGRLAA
                     LIESARARGLTIGLAGSLQARHVPELLVLGPDLVGFRGALCRGGKRGQPLDPVRLACI
                     RGLIPKNRDVAREANLPDRPQLTLC"
     gene            complement(6109..7815)
                     /locus_tag="VFY74_05075"
     CDS             complement(6109..7815)
                     /locus_tag="VFY74_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5931110.1"
                     /translation="MTRSRLRLYLLLCGLLAGAAPIAAMAQDAILLRAREGAAALMRG
                     QYDKAIAAYDEALAAPEIADFIKASIYSDRGVAKWRMNNTKEAIEDFNMSIQLSPENA
                     AVYNNRGNALMDLGHADEAVKDFDRAIALSPNYGAAYNNRGNARVALGQQNLAFQDFR
                     KAAELMPQSAVPFNGRGMAHAELKRYHAAVRDLSRAIGLNPKYVAAYRYRADAYLALG
                     MYREAVADATQVLTLEPDVPNPDILFLRAQAHTGDKKLNFALDDLNKVIEEKPDLVDA
                     YIERAVVFIQAQRTDDAIGDLNRAIELDPQNVRAYAMRASAKLQAEAKDDALYDVNQA
                     LQLATNDPLSLRVRGNIYEALERTDEAITDYRTALAQDPFQAESREALQRLKQEVPAE
                     EGRVLGEPVAGWVIKEPKPGRYIASNPKYPALRTELEMFGTGTPKILEWKLLKDALTG
                     IGLLKYYAGDFGDGIATNLEYVAIVDTRANKVVAVEPHAWGDATAQWNWQAVSVVVTD
                     PDGNANEIQLRKARRAPVARDDFWGFERPGGAPPESRRAARRGGGEGGGGGAGMFNWL
                     FR"
     gene            complement(7891..9417)
                     /locus_tag="VFY74_05080"
     CDS             complement(7891..9417)
                     /locus_tag="VFY74_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX5931111.1"
                     /translation="MLELLTPEEMGRADTLAIESGVAGATLMENAGRAVAEEVARRFP
                     DAGGVAILCGPGNNGGDGFVAARHLMERGYSVRLGFDGDEARLPSDAAAMAERYQGRR
                     EALDDGLIAGADVVVDGLFGAGLARPVEGKLARLIESVNASNLPVVAIDVPSGIDGTT
                     GEVMGAAIRATATVTFFRLKPGHLLLPGRTLCGEVRLADIGIPTSVLAAIAPKTFANE
                     PALWLAKFPWPQPESHKYARGHAVAVSGPVHSTGAARLAARGVLRVGAGLVTVASPRD
                     ALEVNAAQLTAVMVRECDDARGLTSLLADRRRNAILIGPGAGVGERTRELVFAALRSE
                     AAIVLDADALTSFAEDPAPLFAGIAARAAPVVLTPHDGEFSRLFPDLADAPKLERARM
                     GAARAGAILVLKGADTVVAAPDGFASINAMTSPFLASAGTGDVLAGMVLGLLAQAMPA
                     FEAASAAVWLHGAAAVAFGPGLIAEDLPETLPGVLRDLRKLAKSAGRGPIAVRRPITE
                     "
     gene            9704..10042
                     /locus_tag="VFY74_05085"
     CDS             9704..10042
                     /locus_tag="VFY74_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5931112.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEIGLQGITVTEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLELVLGDNMVEKAIDAIIASAKTGRIGDGKIFVSNVEEAVRI
                     RTGETGNDAI"
     gene            10143..11552
                     /gene="glnA"
                     /locus_tag="VFY74_05090"
     CDS             10143..11552
                     /gene="glnA"
                     /locus_tag="VFY74_05090"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX5931113.1"
                     /translation="MSDAKNATKKIKDEEIQFVDLRFTDPRGKMQHVTMDAKVMDEDA
                     FTDGIMFDGSSIAGWKVINESDMKLMPDASTAVKDPFFAMNTLAIFCDVLEPLTNEPY
                     ERDPRSIAGKAQAYLKQTGVGDTAVFGPEAEFFVFDDIRFAVEPFNCTYKIDSMELPT
                     NTDTKYEMGNLGHRPRTKGGYFPVPPVDSAQDIRSEMLATMADMGVITEKHHHEVGAA
                     QHELGIKFGPLVKTADAMQIYKYVIHQVAQAYGKTACFMPKPVYGDNGSGMHTHQSIW
                     KGSTPVFAGDKYADLSPTALHYIAGILKHAKALNAFTNPTTNSYKRLVPGYEAPVLLA
                     YSSRNRSASCRIPLGSGPKAKRVEVRFPDPAANPYLAFAAMLMAGLDGIESKLNPGDP
                     IDKNLYDLPPEELEKVPTVCGSLRQACDELDKDRAFLKKGNVFTDDAIDAYLELKMEE
                     VEKFEMFPHPIEFDMYYSC"
     gene            complement(11702..11998)
                     /locus_tag="VFY74_05095"
     CDS             complement(11702..11998)
                     /locus_tag="VFY74_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931114.1"
                     /translation="MVIRYATLAALAAVSIWALITVGASEGQWLLAGTDEAEGQRNSA
                     FWLLAAIAALMTFSLARFLVLGRPPIVDGWYEQHKPWIYVILGGGLLYAAYVLM"
BASE COUNT         2182 a         3962 c         3923 g         2076 t
ORIGIN      
        1 agcgcaagca ggctcgcccg catgctcggc cgcgacttaa gcgcgggcga cctgctcgcc
       61 tatgtcgatg ttgcgcgcca tttcgccgat catcagcgcg ccgagatcga ggcggcggcg
      121 ctcgcgcttg aggcccgcga gggcctcccc gccgatgctc cggtgattgg cgccggctgc
      181 gggcgtttca ttgcgcagca tatggcggcc cggctcggcc gcccgtattg cgatttcgcc
      241 gagcttttcg attgcgcgcc tcaagcgcgc gacatggcgg ggacgtgcgc gcccgccgtc
      301 gccgtcgggc tgctcgccgc gcatgaactt attctggatg gctgaaggcg gtcgaggtgg
      361 gcgcgctatc cgcgcccacc tcgaagccgc gaactagcgg gtcgccaggg tgtggtacag
      421 ccacaacacg atgatggcgc cgatcgtggc cacgatcatg ctccagatgt tgaagccggt
      481 gacgcccgtc gcgccgagcg ccgtgaacag aaagccgccc accaccgcgc cgacgatgcc
      541 gagcacgatg tccatcaaca ggccctggcc ggtgcccaca taaatcttgc tggcgatgaa
      601 gcccgcgatc agacccaaga caatccaggc aatgatcccc atgaaggacc cctcttggct
      661 gaactcggtt gaagttcgat acgccgttac gacatgagcc gcattacctc ctgccggctc
      721 ggaagtcgac cgtcaggggt aagattatcc accacattcg gcagcatgcg cgcgagttcc
      781 gcgacgactt ggtcgcgggt aagccccgtg cggcgggaca gctcgtcgac gacatccccg
      841 ccgagcgccg cggaaatctg gtccgagccg acgttcttgt tcttgccggt actcacccag
      901 gaatcgacca catcgccgag ccccttctgc tggaactgct tgatgaggcc gccgagccca
      961 tcgacgatgc tgccgggtga cgcatcggtg gattgcggcg cttcctcggg caaccttggc
     1021 gcatcggcct cgggctcctc tttctttttg ccgccgaaat agcgcgaggc gagcagcgct
     1081 agcagagcga tgaccagggg ctttgcgaca ctgccgcctg gaaccgcggg gcctctgtcg
     1141 gatccgaaga tgttctgcac cattttgacc tcctgtttag cacgttacat aaacgccttg
     1201 ccccggggat cgttgcacca ttgcccgagc ggccaaattg gatcactagg acttagggcg
     1261 acgtttcggt gtgtcaagca gcgcttcggc cacatttgaa gaggagcaag cggcagatga
     1321 agccttggat gcttcgcgtt ctgaccttcg cggccgtcac cgcatgcatc ggcgtcggcc
     1381 catcgtatga ggcagcggct ctaccgtctc cacacgtcct caacgccgtc gtggcgccaa
     1441 ctctcgtgga agagggtgga tggcgccgtc agtggcgccg tcagggacca gggccgattg
     1501 gggtcgttcc tgataccggc gtcgtggtcg atccggatgt cgagatcgag tccgaggtcg
     1561 acgttgaggt cgacgtcgag gccgggcccc ccgccgtcat tttcttgccg ccgccacgcc
     1621 cggcgagctg cggacaatac cgctactgga acggcgagcg ctgcgtcgat gcgcgctaca
     1681 acgatcccta tctcggagcg cgatagttca cttttccccg ctctcccctt ggggagagca
     1741 cgcgagcgaa gcgagcggag aggggtcagg cgcgaagcgt cgtcaccaaa agcccccctc
     1801 acccgcccgc cttcgccctt cgggcgatgg cggcaccctc tcccacgagg ggagagggga
     1861 agctcagccc ggcggcggcg gagcgtgctt gtaatggtcc tcgatcttct cgacgagcag
     1921 ccgccaggct tcgtgctcgc gcgggcccgc ggtcttctcc accgcgatct gcaaatagcc
     1981 gagctcgcgc tcgatcttct cgcgcggcag catatgcaag cggcttgcga ggatggcggc
     2041 ctcgatcacc gcgccttgcg cgcggttgag acccttgaac ggggcgtggc tcgcctcatg
     2101 cacgacgcgg cagtggaagc gcggcctttg ctcgtccgcc tcgacttccg tcaccgcaag
     2161 ctcggcatgg gcgagcacgc cttcgagcac ggcgccgggg acatgcgtcg agggccgcgt
     2221 cggccaatcg cgccggccga ccaggcatcc ggcaaagacg agaacgtcgt cggtgaaatt
     2281 cgcgaccgcg taaggcgtga cgccgagatt gtcgagcgtg gtcgagggac ggaatggcgc
     2341 gatgatccag ccgccctcct cggcgatgag accgagcggc gcgatatgaa ccttgccctc
     2401 ttcgctgagg gtggtgacga tgcattcgcg gatcatcggc atggttcagg tgccaggtat
     2461 cagatatcag gtatcagata tcaggtgtcg gtggggtcag tgtcatttcc ttttttggca
     2521 cctgacacct gatacccggc acctgtcttt ctgacacctg acacctgata cctggcacct
     2581 gtcttagcca ctagcgtatg gccggcggcc ttgagctcag tcttatcttc ggccggcgcg
     2641 tcggcggcaa caccccagtc gagcggctcg tcctgcgcgt agcgcttggc cagcctgaaa
     2701 gcaatttcgg ccttgacgag ttcggcgccg agatagaagg cgtgcgcccc gtcgttctcg
     2761 acgctaagct tagggaacag atcgaaggca tcttgcgcca cgtgatggcc gtcgcgattg
     2821 tagatatgaa ttccgtcctc ggcgatctcg atgcggtaat tcttgtcgcg cacctcgcga
     2881 gctgcggcgg cgatctcgtc cggcgtctgc gggaacggcc gccgcgcgtg aacggtcagg
     2941 agcccgtcgg cgtaatcctt gggcaggtcg ccttcggtcc ggctcgcata catgatgcgg
     3001 cgggcgaggt cgtgctcctc caccgtgcgg cgcgtatgcg ggcttacctg caccacgagc
     3061 acgttcttga tgtgcagctc cgaggcgatg ccgagcagaa ccgcggtgat gccggtggta
     3121 tcggcgtcgg tcagctcggt gaggttgccc gtgcccatca gcatctcggc atcggggcgc
     3181 tcgcgcctca cttgcgcgta gcgctccaag gattgcatga agccgaaatt gatgggatcg
     3241 agcacggggt cgacgagata ggcccggccc ttggcatcga gcgcgtccat ggcgcggtag
     3301 agagaagcca tgtcgccgtg ctcttttggg atgagcaccg ggatcgaggc gacctcgtcg
     3361 gcgatggcga gggtggattc gttgaggctc aagagataat cggcgcctgc cttgccgccg
     3421 cgcctgagct cgccagcgtc ggcggagtcg acgctgacct tgtagccttg gcgcctaagc
     3481 gccgaaatgg cgtcctcgag atggggaaac ggcgtatcgg gaagacagcc cagatcgatc
     3541 acgttcgcgc cggcgtccgc gtagtcgcgc gcgcgttcga ggatcgtctc gacggcaagc
     3601 gccgaggcat cgacgatctc ggcgaagatc tggatgtcat acttgctcat gtctgaggct
     3661 ttgcgcgcgc ggccgaaata gagcgggatg tccttgagct cgtcgggccc gcgcaggaag
     3721 ggcacgccga agcgttcgcc caggcgctcg agatcggcgc ggcagcggcc gggcagcatg
     3781 accttgtcgg ccgcgacggg attttgcagc cgccttgtga tgatgtcctc ggtcatcaac
     3841 gccgcaacct tcacgccgat gtccaccacc ttccaatcga agcttgccgt catggcttcg
     3901 agcacggctt cgagccgggc tcgcgccaag tgtccggtca ggaacaggat gctctcggcc
     3961 atgcgcgttt ccgccgttca accctgccgt tctaacccgc ccttggagcg gagaccacaa
     4021 gacgctggaa gtgatacctc gctctcccct tggggcgagc acgcgccgct tgcggcgcgg
     4081 tgagggggca gccggcgaag gcccagtcga aaaattcatc cccttcccgc cactcgccgc
     4141 gcttggcgcg ccgggcggga agccctctcc ctcaagggag agggatcggg attggctatg
     4201 gctcaagtga tcgcggcgcc gaaatcgctc ctgccggtgc ccttggcggc gagcgcggcg
     4261 gcgaaggcct gctggtcgcc gcgtccgagc aagacggccg gcacgcgggc atccttcaat
     4321 atcgaaggaa aggcggcatc gacgacgccg gcttgcgcga gctcctgcgc atccgcgctt
     4381 gtgccctggc gcctgacgga cttcaccaga aaacagcgtg acgcaccgat cctcgcggcg
     4441 agccacagcg caagactgtc ggaggtgaga cgccaggatt cctcgatgtc gctccgcccg
     4501 gcgatcaagc cgtaaggggc ccacacggca acgtctcccc gttcgagagc cgcgcgcagc
     4561 tcgggctcgg tcgtgccgac gacgaagccc tgccgcaagc ccgcaacggc ccaggcgagc
     4621 tgatccatgg cgagaagcgc catgcggtgc gccgcgcgat cgccgaagcc gagcgcggct
     4681 tggacgctgc gaacctcgtc ggcaagagcc cctcctcccg gaaccaccac aacgggacac
     4741 ggcgccgcgg cgatcgcgtc gagccaggcg gcaagctcgc ccgagcggac gacactgccg
     4801 ccgagcttca cgacgaccaa tgctgcttca ccggcgaccg gctcacgttg ggccagctgg
     4861 cgcggaagcg gccgcgccat ggctcagacg tatcccttgg cgcgcacgat ctcgacgccg
     4921 acggcgccgg gttccttgtc gagcttctcg atcctcaccg cgacacgggc cgcgcgggga
     4981 tgagcgaggc agtggcgcgc aatctcctcc gcgagcgtct cgaccaggtt gatatgcccg
     5041 cgggcgacga tgcccttgat gcccgcgatg atcgtgtcgt agtcgaacgt acgggcgata
     5101 tcgtcgtcga gcgctctcgt ggcccgcgtc aagtcgacat cgatcgagaa gcgcacgcgt
     5161 tgggtcgcgc ccttctcgta gttgtggacc ccgatctcga catcgagcac gagatcgcgg
     5221 atgaacacgc ggtcgagcgc cggcgcttca tcgggcgctg cgttcggcgc tacctgcctc
     5281 aatttcggct cggccatgac caggctccat tgcggcgcgt caatctcact gcgccatccc
     5341 taacataaag tgagttgggg acgatccggc aagttcgctt cgcgcgcaac gtctcgattt
     5401 ttcgggatca gccccctgat gcaagcgagc cgcaccggat cgagcggctg accccgcttg
     5461 cccccgcggc agagcgcgcc gcgaaagccg acaaggtcgg gcccaaggac aaggagttcc
     5521 ggcacgtggc gcgcttgaag cgaaccggca aggccgatcg tgagccctcg cgcccgcgcg
     5581 gattcgatca aggccgcaag cctgccggcg gaaacgaggt cgggcagcga gacgccgctc
     5641 ttgccaaaag tatccagcat cacgccggag gccccgatgc gcgcagcaag ccttatcgca
     5701 tcgatctccg aaagagcgtc ggcaaacaga accaggatca accggatttg gggcgtgagc
     5761 gacgcgagca tggtgaggca gcgttccccg tcccctcccg gaaaaagccc gagcttgacg
     5821 tagtcgacgc cccgcgccgc catcgacagc acggcggcgc gaaccccctc ctgctcaagc
     5881 ggcacatcgc cgatggttgc gctcaccggc acccggccgc cgatcgccct cacgcaagcg
     5941 tcgactgtcg caggcgcgag agcgccgaga gcgcccgcgc ccggatcctt gagatcgacg
     6001 atgtcggcgc cggcgccgat cgccatttcg gcctcgccgg cgtcgcgcac actcgccagg
     6061 aagagggtca tggcccggcg ttgtccggcc ggcagcccgg ctcaagcatc agcggaacag
     6121 ccaattgaac atgccggcgc cgccgccgcc gccttcgccg ccaccccgcc gcgcggcacg
     6181 ccggctctcg ggtggcgcgc cgcccggccg ctcgaagccc caaaaatcgt cgcgcgccac
     6241 cggcgcccgc cttgctttgc ggagctgaat ctcgttggcg ttcccgtccg ggtcggtcac
     6301 gaccacggac acggcctgcc agttccattg tgcggtcgcg tctccccagg catggggctc
     6361 cacggcgacg accttgttgg cgcgggtatc gacgatggcg acatattcga ggtttgttgc
     6421 tatgccgtcg ccgaaatcgc cggcatagta tttcaagaga ccgatgccgg tgagagcgtc
     6481 cttgagcagc ttccactcga gaatcttggg cgtgccggtg ccgaacattt caagctcggt
     6541 cctgagcgcc gggtatttcg gattgctcgc gatatagcgt ccgggcttcg gctccttgat
     6601 cacccaacct gcgaccggct cgccgagaac gcggccttcc tcggcgggca cctcttgctt
     6661 gaggcgctgg agcgcttcgc ggctttcggc ctggaatggg tcctgcgcaa gggcggtgcg
     6721 ataatcggta atcgcttcgt cagtgcgctc aagcgcctcg tagatgttgc ctcgcactct
     6781 gagagagagc ggatcgttgg tcgcgagctg aagggcctgg ttgacgtcat agagcgcgtc
     6841 gtccttggcc tcggcttgga gcttcgccga ggcgcgcatg gcgtaggccc gcacgttctg
     6901 aggatcgagc tcgatggcgc ggttcagatc gcctatggcg tcgtcggtcc gttgcgcctg
     6961 tatgaacacg acggcgcgtt cgatataggc atcgacgagg tcgggctttt cctcgatgac
     7021 cttgttcaga tcgtcgaggg cgaaattgag cttcttgtcg ccggtgtggg cctgggcgcg
     7081 caggaagaga atgtcggggt tgggaacatc gggttcgagc gtgaggacct gggtggcgtc
     7141 ggccacggct tcgcgataca tgccgagcgc gagataggcg tcggcgcggt agcgataggc
     7201 cgccacatat ttcggattga ggccgatggc gcgactgagg tcgcgcacgg cggcgtggta
     7261 gcgtttcaat tcggcgtggg ccatgccccg cccgttgaac ggcacggcgc tctgcggcat
     7321 cagctccgcg gccttgcgaa aatcctgaaa ggcgagattc tgttggccga gcgccacccg
     7381 ggcgttgccc cggttgttgt aggcggcgcc gtagttcggc gacagcgcga tggcccggtc
     7441 gaaatctttc accgcttcgt cggcatgacc cagatccatg agcgcattgc cgcgattgtt
     7501 gtagaccgcg gcgttctccg gcgaaagctg gatcgacatg ttgaaatctt cgatcgcctc
     7561 cttggtattg ttcatgcgcc acttcgcgac gccgcgatcg ctgtagatgc tggccttgat
     7621 gaaatcggcg atctcggggg cggcaagagc ctcgtcatag gcggcgatcg ccttgtcgta
     7681 ttgaccgcgc atgagcgccg cggccccttc gcgggcgcgc aacaggatgg cgtcctgcgc
     7741 catggcggcg atgggtgccg cgccggccag gaggccacaa agcagcaggt aaagtcttaa
     7801 ccgcgagcgg gtcatggcga tgatcaggcg gctgtccccc ggagcgtctg ctccatgtcg
     7861 gtcataaggc cggactatgt caacaacagg tcactcggtg attgggcgcc taacagcgat
     7921 tgggcccctt cctgcacttt tagccaattt ccgaaggtcg cgaagcaccc ctggcaaggt
     7981 ctccggcagg tcctccgcga tcaggccggg gccgaaagcg acagccgccg ccccatgcag
     8041 ccacacggcg gcgctcgccg cctcgaaggc gggcatggct tgggcaagaa gcccaagcac
     8101 catgccggcc agcacgtcgc cggtcccggc gctcgccagg aaggggctcg tcatggcgtt
     8161 gatcgaggcg aagccatcag gcgcggcgac gacggtgtcg gcgcccttca gcacgaggat
     8221 ggcgcccgca cgtgccgcgc ccatccgcgc ccgctcgagt ttgggcgcat cggccagatc
     8281 aggaaacagg cgcgaaaact ctccgtcatg aggcgtcagc acaaccgggg cggcgcgggc
     8341 ggcgatgcct gcaaagagcg gcgcgggatc ttcggcgaaa gacgtgagcg catcggcgtc
     8401 gagaacgatc gcggcctcgg agcgaagcgc cgcaaagacc agttccctgg tgcgctctcc
     8461 gacacccgcg ccagggccga tcaggatggc gttcctgcgc cggtcggcga gaaggctcgt
     8521 cagcccgcgg gcatcgtcgc attcccgcac catgacggcg gtcagctgcg cggcattgac
     8581 ttcgagcgcg tcgcgcggcg aggcgacggt gacgagcccg gcgccgacgc ggagcacgcc
     8641 gcgagccgcg agccgcgcgg ccccggtgct gtggaccggc cccgacacgg caaccgcgtg
     8701 tccccgcgca tatttgtggc tctcgggctg cggccaggga aactttgcaa gccatagcgc
     8761 cggctcattg gcgaaggtct tgggcgcgat ggcggcaaga acgctcgtcg gaatcccgat
     8821 atcggcgagc ctcacctccc cgcaaagcgt ccgtccaggt agaagcagat gacccggttt
     8881 caggcggaag aacgtcacgg tcgcggtggc gcgaattgcg gcgcccatca cctcccccgt
     8941 cgtcccgtcg atgccgctcg gcacgtcgat ggcaacgaca ggcaggttcg atgcgttgac
     9001 gctctcgatc aggcgggcga gcttgccctc gacggggcgg gcaaggcccg cgccgaacag
     9061 gccgtcgaca accacatccg caccggcaat caggccgtca tcgagcgctt ctcgcctgcc
     9121 ctgatagcgc tcggccatcg cggccgcgtc ggaaggcaag cgcgcctcgt caccatcgaa
     9181 tccgagcctc acgctatagc ctcgctccat gaggtggcgc gcggcaacga acccgtcgcc
     9241 gccgttattg ccgggaccgc acaagattgc gacgcccccg gcatcgggga agcggcgcgc
     9301 gacctcctcc gcgacggcgc ggccggcatt ctccatgagg gtcgcgccgg cgacaccgct
     9361 ctcgatggcg agagtgtcgg cgcgccccat ctcctccggc gtcagaagct cgagcatggt
     9421 ctatcgcggg tcatgagcgc ctcggtccag aacaacagtc tcaagacaaa gagttgcaaa
     9481 cctgcttaat ttttgtgcat agagtccatg ccttgagcat tcctccgttt cccgccacaa
     9541 acagccgcgg ctcaactccc gcaaggtatt gaaacgtaat acgaaacttg ccccttgagg
     9601 attggcatgg ggcatgcttt ggctgggcca cggtttgaga gacacagacg gaagcgcgca
     9661 ggctggaagc gtcctttgcg ttccggggag gttcatcgaa acaatgaaaa agatcgaagc
     9721 gatcatcaag cccttcaagc tcgatgaggt gaaagaggcc ctgcaggaga tcggcctcca
     9781 aggcattacc gtgaccgagg ccaagggctt cgggcgccag aagggccaca ccgagctcta
     9841 tcgcggcgcc gaatacgtcg tcgactttct gcccaaggtg aagctcgagc tcgtgctcgg
     9901 cgacaacatg gtggagaagg cgatcgacgc catcatcgcc tcggccaaga ccggccgcat
     9961 cggcgatgga aagatcttcg tatcgaacgt ggaagaagcc gtgcgcatcc gaaccggcga
    10021 aacggggaac gacgccatct gaggaggata atgaggcttc aggccggccg atgccggtga
    10081 gatcgagttc caccgccaca tgaaaccggc ggcatccatt acgccaatca caggggagaa
    10141 cgatgtcaga cgccaagaat gcgaccaaga agatcaagga cgaggaaatt cagttcgtgg
    10201 atttgcgctt caccgatccg cgcggaaaga tgcagcacgt gaccatggac gcgaaggtca
    10261 tggacgagga tgcgttcacc gacggcatca tgttcgacgg atcctcgatt gcgggctgga
    10321 aggtgatcaa cgagtcggac atgaagctca tgcccgacgc gagcaccgcg gtgaaggacc
    10381 cgttcttcgc catgaacacg cttgccatct tctgcgacgt gctcgagccc ttgaccaacg
    10441 agccctacga gcgcgatccg cgctcgatcg ccggcaaggc gcaagcctat ttgaagcaga
    10501 ccggcgtcgg cgacaccgca gtgttcgggc cggaagccga gttcttcgtg ttcgacgata
    10561 tccgtttcgc cgtcgagccg ttcaactgca cttacaagat cgactcgatg gagctgccga
    10621 ccaacaccga caccaagtac gagatgggga atctcgggca ccggccgcgc accaagggcg
    10681 ggtatttccc cgtgcccccg gtcgactcag cgcaggacat tcgctcggag atgctggcga
    10741 cgatggccga tatgggcgtc atcaccgaga agcatcacca cgaagtgggc gccgcccagc
    10801 acgagctcgg catcaagttc ggcccgctgg tcaagaccgc cgacgccatg cagatctaca
    10861 aatacgtcat ccaccaggtg gcgcaggcct acggcaagac cgcctgcttc atgccgaagc
    10921 cggtctatgg cgacaacggc tcgggcatgc atacccacca atcgatctgg aagggttcga
    10981 ccccggtctt tgccggcgac aaatatgccg acctctcgcc gaccgcgctg cactacatcg
    11041 ccggcatcct gaagcacgcc aaggcgctga acgccttcac caacccgacc accaactcgt
    11101 acaagcgtct ggtgccgggc tacgaggcgc cggtgctgct ggcctattcc tcgcgcaacc
    11161 gctcggcctc atgccgcatt ccgctcggct ccggaccgaa ggccaaacgc gtcgaggtgc
    11221 gcttccccga tcccgcagcc aatccctatc tcgccttcgc cgccatgctc atggccggcc
    11281 tcgacgggat cgagagcaag ctcaaccccg gcgatccgat cgacaagaat ctttacgact
    11341 tgccgcccga ggagcttgag aaggtgccga cggtgtgcgg ctcgctgcgg caggcttgcg
    11401 acgagctcga caaggaccgc gcctttctca agaagggcaa cgtcttcacc gacgacgcga
    11461 tcgacgccta tctcgagctc aagatggaag aggtggaaaa attcgaaatg ttccctcacc
    11521 ccatcgagtt cgacatgtat tacagctgct gacgccagat acgtttcgcc aaaaagttgg
    11581 ggcggccaac tcgcatgggt ggccgcccca ctttcgagcc cggcctgccg aaccttcctt
    11641 cgcggtcccc cgaggccgcc gcaagaaggt tctcgccagg ctcccccaaa acgcggatcg
    11701 cttacatcag cacgtacgcc gcatagagca gccctcctcc gaggatgacg tagatccacg
    11761 gcttgtgttg ttcgtaccag ccgtcgacga tcggcggccg ccccaggacg agaaatcgcg
    11821 cgagcgagaa ggtcatgagc gcggcgatgg cggccaggag ccagaacgcg ctgttgcgct
    11881 gaccctcggc ttcatcggtc ccggcgagaa gccattggcc ctcgcttgcg ccgacggtga
    11941 tgagggccca gatcgagacc gctgcgagcg ccgccaaagt ggcgtaacga ataaccatga
    12001 cgcgcaaaac tccgccgctt tgagacgaat tttaggaacc ggcgggtaaa gaaacgggaa
    12061 agcggcaggc ttaagcaggc gttaacgggc gtgacgcctc aggcggcctt ccatttgatg
    12121 gagcatccga tggacgcgat ctg
//
LOCUS       DASUQP010000114        23694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_869846,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000114 DASUQP010000000
VERSION     DASUQP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 23694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23694
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869846"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..2329
                     /locus_tag="VFY74_05100"
     CDS             143..2329
                     /locus_tag="VFY74_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5931115.1"
                     /translation="MLASVLPFSMLPLETAMAAGEAAALDARTLLTLGINLGIIAFAV
                     GTAIACLRATERARKAEAAAEREAERYRASEGMLDALLAAEPQALLAVSESGEAELVI
                     ATLPAALGVPNDTAELLNFAAWLDTASAKGLDEAIDGLVERGEAFNVMLRTQRDRYIE
                     ADGRAAGRTIVLKMRELPGQRLDLAELASRHRQLEEQVGSLRALLEEAKKPARIPVEP
                     KPHLEARFRSFDRLATAFAVFDRGQRLTHFNQAYVALWQLAPEWLATHPRDGEILDRL
                     RQARRLPEKADYRGWKHDWLAAYGKNVQAEDLWHLPDGRTLHVVADSEGEAGVTYLYE
                     NVTERLMLESRYNALIEVQRETLDTLREGVAVFAPSGSLRLYNRAFAAIWRLNPRQLD
                     GEPHIDEIIAWCRALYDEPEEWERIKAAVTAIVAARVAYEKQFDRTDGSVLACAVLPL
                     PDGGTLFTSVDVSDAKHAERALKERTEALEAADRLKTAFISHVSYELRTPLTNIIGFS
                     ELLASPAAGPLTDRQREYLGDIRLSGRTLLSIIDDILDLATIDAGTLELKLSPVNVSH
                     VIEQAAQGLAERIKLNDLGLDIDIEPGIDDFVADGKRVTQMLYNLLSNAIGFSDTGGK
                     IALRCGRRDSMIAFTVADQGVGIPEDYQQAVFDRFESRSYGSRHRGAGLGLSVVKSLA
                     ELHGGTVTLDSGPGRGTRVTLLLPYRLEPAGESSEPDAAHYTSRAG"
     gene            2326..3852
                     /gene="tsaE"
                     /locus_tag="VFY74_05105"
     CDS             2326..3852
                     /gene="tsaE"
                     /locus_tag="VFY74_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX5931116.1"
                     /translation="MTASVSLDDVDLARLDIIAGRLALMLKSGDVVALSGPLGAGKTT
                     LARALIAGLGGATEVPSPTFALMQRYELPHLTLTHCDFYRLESHELDELGLDDAVSEG
                     VVIVEWPERAATWLPHDRLDVAIEETAKPDARRIVLTGHGGWEKRLERLKALSDFLDG
                     TPYAEAPARYLLGDASTRSYARLALPGRPAILMNSPRQPDGPPIRGGKPYSALVHLAE
                     DVTPFVAVAGTLKEAGLSAPIVYAFDLDRGLMVLEDLGDAVFGSEVRRGRALSELWGP
                     AVDVLVALAENEPPRLLPIAGHAPYRLPRYDAGAMLIEASLLMDWFWPAVHGRAAPPS
                     LVSEFEALWRPLLSDAERDDPGWVLRDYHSPNLMWLPERQGIERIGILDFQDALRGPL
                     AYDLVSLLQDARLDVPEALERELLARYCAARGVGSKSFSRDRFVSLYAVLGAQRNSKI
                     LGIFARLAKRDGKRGYLAHIPRVARYLERDLAHPSLAELRRFYAREFPSAENLPPLVV
                     "
     gene            3865..4590
                     /locus_tag="VFY74_05110"
     CDS             3865..4590
                     /locus_tag="VFY74_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5931117.1"
                     /translation="MTEARTAMVLAAGLGERMRPLTLTMPKPLVRLAGKPLIDHVLDR
                     LADAGVKTAVVNVHYLPEQLEAHLASRRGRPPETLVSDERGVLLDTGGGTSRALPLLG
                     QGPFFIHNADSVWTEGASPALSRMLKKWNPALMDSLLLLAPVTTSVGYAARGDFAMSQ
                     DGRLARRGANEVVPFAFAGVSLCDATLFKNAPEGRYSLNLLWDRALAKGKLYGIRLDG
                     RWMHVGTPAALAEAETAFEREGA"
     gene            4583..7714
                     /gene="addB"
                     /locus_tag="VFY74_05115"
     CDS             4583..7714
                     /gene="addB"
                     /locus_tag="VFY74_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEX5931118.1"
                     /translation="MPETPPERPRLYSIPPSAPFLTTLAKAILQGELPSPGGPKPGPL
                     TLPLTTIYLPTRRAGRALREAFLAETEGEALLLPRIRALGDPDEDAAIIFGTGDFPDG
                     DGAMEAPAIGSLRRRLALMRLVLAFGERLGAEAAAESGDGAAPTFALTPGQASHLAAD
                     LARLMDFIESEEVALSALKEIVPEEFAGHWQLTVEFLKIVTEQWPRYLADNGLISPVA
                     RRTYLMAVETERLLKGSTYPVIAAGSTGTVPATARLLKAIASVPNGAVVLPGLDSFLD
                     DETWSRLADEPEHPQFGMAELLRKLGATRADVADVPGALPDPAGRARLHLASEALRPA
                     DSTDRWERFLAGDDLSAGGKASLGRASLANATSGIHLVVAPTAHDEAEAIALMLRSAV
                     ETPGKTAALVTPDRTLARRVAARLKRYDLAVDDSAGIPVAHTVPGAFLDLVLGAAETN
                     FGPPELMALLKHPLALLGRSPGEARSAARALERGTFRGVYVGEGLEGARAALHAAQSE
                     ERRGPVALSSSEHAGALRLVADLETAFAPLIALAGPLPQPAGRLAEVHAAVAEALARD
                     AAGSSSALWQGEAGEALSVLLAELIDAGRAVTLDPPDYPAFYRSLVAGDVVRPRAGLH
                     PRLFIWGPLEARLQQPELVILGSLNEGVWPRPEEAGPWLSRPMRASLGLPPPERRIGL
                     SAHDFAQALGAREVYLTRALKVDGVPTVPSRWLQRLLALVKAAGLEAKLAPAEPWVAW
                     ARERDTAPPFDPVTAPAPRPPLEARPRKLSVTRIERWIANPYEIFAKDILKLEPLNPL
                     SIEPDAALRGQIVHRALHEFSHAYPDRLPDDIEEALVAIADRHFAALAGSPVVKAFWR
                     PALRRFARWFAATEPARRSGVTKIVTEAYGTFDLAPSFRLTARADRIDIAEDGSAVIY
                     DYKTGKPPLQKYVDELFSPQLPLEALIAEEGGFPDLGKRAVSGLRYIHVTGRAEGGVE
                     CEAGKNAPADLASEARAKLTRLIARYADPAMPYEVKRRSAPAFRQIYKYDAYEHLARI
                     KEWLTLEAEEDFG"
     gene            7711..11211
                     /gene="addA"
                     /locus_tag="VFY74_05120"
     CDS             7711..11211
                     /gene="addA"
                     /locus_tag="VFY74_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HEX5931119.1"
                     /translation="MSSCAPLPPEADANQARASDPAASAWVSASAGTGKTEVLVKRAL
                     RLLLAGFKPESILCLAYTKTAAAEMQNRLLGELAGWATAKSEVLACKLNELTRQAPDA
                     ETLRLARRLFARALEAKGGLKIHTIHGFCERLLQRFPLEARVTPHFKVLDEREQASLR
                     RAAFDATLARAAEESEAPLGKALATVMAFTSGDYVRQVIDTVMAKRAALARIVDYHAG
                     IAGWAEAEALALKRLFGVGDETEETLLAQLASVLDDDAIHAAIAAFAAFCSVARTDKE
                     AEASLRQARASAGDRRVAALAEMFLTQDGKPLSQLCTTRFAAAEPALAASLKQAQAVF
                     AGLKDKLAHLRMAEASAAVLAIADAVRDAYERRKLPDVALDYDDLIDKAQNLLSRADA
                     ASWVLYKIDGGIDHILVDEAQDTNPAQWTIIENLAAEFFAGSGRSDRLRTLFAVGDEK
                     QSIYSFQGANPMRFGEVGRAFQKRATALSLDWHTVPLTLSFRSTEPILDAVDRVFARA
                     PATNGLIFTEGGAAITHQAFRKGEAGLVELWTIETEEEAEPADAFEPWNEELAGARAV
                     DALARRIAATIKSWIEQGEELPAQGRNVRAGDILILVRRRDPFTTPMIRELKRLNVPV
                     AGADRMKLTDQIAVQDLIALADVLLMPEDDLALAVVLKSPLFGFDDNDLFALAYRRPA
                     SLWSALKDKAKDDTRFAEAVKKLSSLLARADFLPSYEFFAELLGADGQRLRKRMLTRL
                     GPEAAEAIDEFLDRALAYDRDSPPSLQGFVNALRAGDVEIKRDMEQQRDEVRIMTVHG
                     AKGLQAPIVFLPDTCTRARTQGPRLYPFAREGASAEAVDHLVWPPTGHSKLATLDESK
                     SILERAERDEYHRLLYVAMTRARDRLYVCGWQGKQQRENNSWYDLIKDGLGSLAEHDA
                     PGGKKVGRMHSAQTKPVERVEEKREADATAPLPEWALAPAPLERTRPKLAPSRLALFG
                     EPRAEPFPDQTPLSPRRLGERSRFARGRLVHALLQHLPEIAPGEQERVARSFVAARGA
                     DLSPELREEIVAETLAIARDALFAPLFQPGSLAEVPVVARIGGYDLEGQIDRLAMVED
                     SLLIVDYKTNRPPPRTLDEVAPAYIAQLAGYRKALAEMFPGRRLRTALLWTDGPTLME
                     IPATSLDAAEERLFNPPVEP"
     gene            11290..11616
                     /gene="trxA"
                     /locus_tag="VFY74_05125"
     CDS             11290..11616
                     /gene="trxA"
                     /locus_tag="VFY74_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5931120.1"
                     /translation="MATEKVSDDTFEAEVLKSPTPVVVDFWAEWCGPCRQIAPALEEI
                     ARELNGKVKITKLNIDENPRTPSHYNVRAIPTLILFKDGEVAGTLKGAYPKNHLAAWI
                     NGTAGA"
     gene            complement(11633..12985)
                     /locus_tag="VFY74_05130"
     CDS             complement(11633..12985)
                     /locus_tag="VFY74_05130"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX5931121.1"
                     /translation="MTEFPHSAGRASPRPRSASSDSLLERLKALHPQSIDLSLGRIER
                     LLAALGHPEHKLPPVLHVAGTNGKGSLLAFARAIAEALGKRVHVYTSPHLIHFHERVV
                     LAGPHGSGPIAEDLLVDCLARAEAANDGELITLFEITTAAAFLAFAETPADYLLLETG
                     LGGRLDATNVVSRPALTAITPVSIDHVSFLGETLTEIAGEKAGILKPGVVCVVGRQEQ
                     EAFDVIEARAEQVGAPLQVAGRDFDTYEQHGRLIFSTPTRLIDLPLPRLKGRHQIDNA
                     GTAIAAAERLFGETLTTRALEQGMIHAEWRARLERLPAGSLHAYVADGTEIWLDGGHN
                     PAGGRAIAQALAELEERVPRTMHLVFGMMETKDAHAFIAPFKGLVERVYTVPIPNEPN
                     AFSAEALADIARAGGFDVTVASSVPDALLKSQAALPGAGRVLICGSLYLAGHVLKLHG
                     "
     gene            complement(12998..14017)
                     /gene="accD"
                     /locus_tag="VFY74_05135"
     CDS             complement(12998..14017)
                     /gene="accD"
                     /locus_tag="VFY74_05135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX5931122.1"
                     /translation="MNWINSVVRPKIRSILRKKDLPDNLWVKCPETGQMIYYKDLESN
                     QFVVPGSNYHMRMSATARLKSLFDNGQYETLPLPEVPIDPLRFRDERRYTDRLKDARN
                     KTGLSDAILSGAGRLEGQTVIAAVQDFDFLGGSLGTAAGEAMISVMRAAVKRETPFIL
                     FAASGGARMQEGILSLMQMPRTTVAVQELREARLPYIVVLTHPTTGGVTASYAMLGDV
                     HIAEPGALIGFAGPRVIEQTIREQLPNGFQRAEYLHEHGMVDMVVHRHQLRGTLSRLC
                     HLLTHRGRDLSLSEMKDGNLSRAAPPSTIALPGNGLDATHEEDADDLTDTAGDGATVN
                     PPRGV"
     gene            complement(14051..14887)
                     /gene="trpA"
                     /locus_tag="VFY74_05140"
     CDS             complement(14051..14887)
                     /gene="trpA"
                     /locus_tag="VFY74_05140"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5931123.1"
                     /translation="MTRIDERFAALKREGRAGLVAFVTAGDPDYETSLAILKGLPASG
                     ADIIELGMPFSDPMADGPAIQASSQRALKAGQTMKKTLQMVKAFRAADGATPLVLMGY
                     YNPIYVYPVDRFIADAAEAGADGLIVVDMPPEEDAELRPAAAAAGLNFIRLATPTTDA
                     RRMPAVLANTSGFVYYVSIAGITGTKAPDLNEVKGHVARIKAQTSLPIAVGFGVKTEG
                     QVSAIAGIAEGVVVGSALVTAIAQSLDQEGNATRATGPQVLGLVERLAAALRSPAEAA
                     VL"
     gene            complement(14890..16122)
                     /gene="trpB"
                     /locus_tag="VFY74_05145"
     CDS             complement(14890..16122)
                     /gene="trpB"
                     /locus_tag="VFY74_05145"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5931124.1"
                     /translation="MSVRDQPNPLNTYRGGPDERGHFGLYGGRYVAETLMPLILDLER
                     AYAEAKEDPAFHAELVDLLTHYAGRPSPLYFAERLTSQLGGAKIYFKRDELNHTGSHK
                     INNCLGQIQLARRMGKTRIIAETGAGQHGVAVATVAARFGLPCVIYMGATDIKRQRPN
                     VFRMKLLGAEVKPVTSGSGTLKDAMNDALRDWVANVHDTFYIIGTVAGPHPYPAMVRD
                     FQSVIGRETREQMQEREGRLPDTLVACIGGGSNAMGLFHPFLDEPSVAIYGVEAGGYG
                     LDVANGHAASMSGGEPGVLHGNRTYLLQNDDGQILEGHSISAGLDYPGVGPEHSWLRD
                     TGRVNYVAVTDKEALDAFQLCTRLEGIIPALEPSHALAYVKKLAPTLPRDNLLVMNMC
                     GRGDKDIFSVAEHLRMEM"
     gene            complement(16119..16784)
                     /locus_tag="VFY74_05150"
     CDS             complement(16119..16784)
                     /locus_tag="VFY74_05150"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5931125.1"
                     /translation="MPAKVKICGVRTLAIVETAVEAGADYVGFVFFAKSPRNLTLQAA
                     KVLALAARGRVGTVAVLVDADDAVIDDVVETVRPDLLQLHGGETPERVAAIKACTRLP
                     VMKAVAIAEASDVAAARAYAAIADCVLFDAKADAGATLPGGNGVAFDWRVLKGFGTPF
                     ALSGGLTPETVAMAISMTGAELVDVSSGVERSPGEKDAELVKRFIRAAKGAVSSKPRA
                     KAS"
     gene            complement(16882..17115)
                     /locus_tag="VFY74_05155"
     CDS             complement(16882..17115)
                     /locus_tag="VFY74_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931126.1"
                     /translation="MDALTLFGLLAVTAMLVFYALEDLSPWFILAFAAACGLASIYGF
                     LQGAWPFGVVEAIWAGVAVWRWRDRTFGRGPRR"
     gene            complement(17130..17477)
                     /locus_tag="VFY74_05160"
     CDS             complement(17130..17477)
                     /locus_tag="VFY74_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX5931127.1"
                     /translation="MWRKLVLALVVIPLGVVLVTLAIVNRKPALLVLDPFGGSEGGLS
                     LEAPLFLFLLGAFALGLVVGGIATWLRQGKWRKTAREGAREARELRRQADRLEKELEG
                     AGSAPARPRLAVD"
     gene            complement(17500..17790)
                     /gene="ihfB"
                     /locus_tag="VFY74_05165"
     CDS             complement(17500..17790)
                     /gene="ihfB"
                     /locus_tag="VFY74_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX5931128.1"
                     /translation="MIKSELIQKVSGANPHLFHRDVERIINIVLDEITGALARGDRVE
                     LRGFGAFTVKHRAARQGRNPRTGDTVFVEDKYVPFFKTGKELRERLNPGGTR"
     gene            complement(17845..18807)
                     /gene="sppA"
                     /locus_tag="VFY74_05170"
     CDS             complement(17845..18807)
                     /gene="sppA"
                     /locus_tag="VFY74_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX5931129.1"
                     /translation="MALDAESVIDRRRLKRRVTAWRIAAVALGLIALGLLLLGDRNVS
                     GSGSFLPHVARVTVSGIITDDRKMNELIDKVGKSDQVKAVIFNIDSPGGTTTGGEAMY
                     DSIRRLAEKKPVVAVCGTLATSAAYIAAIATDRIFVYGNTITGSVGVVFQWAEVTDLL
                     KTLGIKVEEVRSGPLKAIPSPFEPADEKARALTQEMVQEARVWFVDLVSKRRNIEAPS
                     IPGLTDGRIYSGRQAVELKLADEIGDEKTAMTWLTKERKVPPGLKVVDWKPKVESSSL
                     YGWFFESLAPMVGLLPERLAGIAAQISASLKLDGLVSVWHPASD"
     gene            complement(18854..20647)
                     /gene="rpsA"
                     /locus_tag="VFY74_05175"
     CDS             complement(18854..20647)
                     /gene="rpsA"
                     /locus_tag="VFY74_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX5931130.1"
                     /translation="MTEPQTSVSHPSRDEFAALLEESLTGTALQEGSVVKGTIVAIEK
                     DVAVIDVGLKTEGRIPLKEFGTSGRSPDLSVGDEVDVYLERVENSAGEAVLSREKARR
                     EESWTRLEEKYKSGERVEGVIFNRVKGGFTVDLDGAVAFLPGSQVDIRPIRDVGPLMN
                     VPQPFQILKMDRRRGNIVVSRRSVLEETRAEKRSEIVARLEEGQVIDGVVKNITDYGA
                     FIDLGGIDGLLHVTDMAWRRVNHPSEIVNVGDTVKVQIIRINPETQRISLGIKQLEAD
                     PWEGIAAKYPTGSRFRGTVTNITDYGAFVELEPGVEGLIHVSEMSWTKKNVHPGKIVS
                     TSQEVEVQVLEVDPDKRRISLGLKQTQENPWVAFTANHPPGATIEGQIRNITEFGLFI
                     GLDGGIDGMVHLSDLDWSKSGEDALKDYKKGDVAKAVVLEADSEKERISLGIKQLTPD
                     PFAAGAPSAKLKKGMRVTCEVLQVKDNGLEVKLVDHDLTTFIRRSDLSRDRSEQRPDR
                     FGVGEKFDAVVTQVDAKSRKVSVSIKALEIAEEKEAVAQYGSTDSGASLGDILGAALN
                     KAKGDEGDEPVKPKRKRPAKDDPEPSTAPEE"
     gene            complement(20842..21483)
                     /gene="cmk"
                     /locus_tag="VFY74_05180"
     CDS             complement(20842..21483)
                     /gene="cmk"
                     /locus_tag="VFY74_05180"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5931131.1"
                     /translation="MLIAIDGPAASGKGTLGRRIAAHYGLAYLDTGRLYRAVARDVLA
                     TGASLSDANAALAAAKALNPATLDDPTLMDGKLGEAASIVASRADVRGALLAYQRAFA
                     GKAPGAVLDGRDIGTVICPDADVKLFVTASAEVRAERRYRELRLAGHDMTEAEVLADI
                     ARRDRRDKSRAAAPLRRARDAYLLDTTNLAIDAAFKAAIALIDAAMGRAGQAN"
     gene            complement(21477..22802)
                     /gene="aroA"
                     /locus_tag="VFY74_05185"
     CDS             complement(21477..22802)
                     /gene="aroA"
                     /locus_tag="VFY74_05185"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5931132.1"
                     /translation="MKQPLTARSSARLSGRIRVPGDKSISHRALILGALATGTTRVSG
                     LLDAEDVAATAKAVSALGAEVERLNGGVAVKGQGVGGLRAPASVLDFGNSGTASRLML
                     GVIAGHDMQVELTGDASLRRRPMGRVLAPLAAMGLQAESESDRATLPLVVRGTSDLLP
                     IVYDLPVPSAQVKSAVLLAGLHAPGRTTVVEPLAARDHTERMLGFFGAEIVAEDRGEG
                     ARAVTVGGDAELHGASIAVPGDPSSAAFMIAAALIAPGSDIVVEGVLLNPTRTGFIET
                     LLEMGANIDILDRRDEGGEPVGDLRVRASALQGVRVPRERAPSMIDEYPILACLAAFA
                     EGETAMEGLAELKVKESDRLAATEQGLAACGVSVRVEGDDLFVQGEGRVKGGARVETK
                     MDHRIAMAFLTLGLGARAPVTVDDVGMIGTSFPGFVPLMTRLGANFASC"
     gene            22905..23306
                     /locus_tag="VFY74_05190"
     CDS             22905..23306
                     /locus_tag="VFY74_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX5931133.1"
                     /translation="MSKPARGTKRVCPSCGARFYDLNRTPITCPVCQAVYQLPTAPSR
                     RGERASAAGARKVEEPPPRPVEPVEVISMEEVEEAGAEATIAEEEEIVDLGDDAPEIP
                     PGDDDNAFLEEEEEGEADVTGIVGGDKGEEG"
     gene            23514..23589
                     /locus_tag="VFY74_05195"
     tRNA            23514..23589
                     /locus_tag="VFY74_05195"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23547..23549,aa:Ala,seq:ggc)
BASE COUNT         4002 a         7854 c         7773 g         4065 t
ORIGIN      
        1 tcgccttgag gctcgcgggg tcaatcggcc tgcgttcagt ctgtggcagc ggaagcgagg
       61 cgaggccatg ggcggggcgt gggagcgagg gaccatgcgg accagcgggt gtaagcttgc
      121 accttcatca aggtggccga cggtgcttgc ctccgtgctc cccttctcca tgcttcccct
      181 tgaaaccgcc atggccgcgg gcgaagcggc ggccctcgat gcccgcacgc tgctcacgct
      241 cggcatcaat ctcggcatca tcgccttcgc cgttgggacg gccatcgcct gtctccgcgc
      301 tacggaacgg gcgcgcaagg ccgaggccgc cgccgaacgc gaggccgagc gctatcgcgc
      361 cagcgagggc atgctcgatg cgctgcttgc ggcggagccg caggcgcttc ttgccgtgtc
      421 tgagtcgggc gaggcggagc ttgtgatcgc caccctgccg gcagcgcttg gcgtaccgaa
      481 cgacaccgcc gagctcctca atttcgccgc atggctcgat accgcctccg ccaagggcct
      541 cgacgaggcc atcgacggac tggtcgagcg gggcgaggct ttcaacgtga tgctgcggac
      601 gcagcgcgac cgctatatcg aggccgacgg ccgcgccgcc ggccgcacca tcgtgctcaa
      661 gatgcgcgaa ctgcccggac agcggctcga tcttgccgag cttgcgagcc gccaccggca
      721 gctcgaggag caggtgggct ccttgcgcgc gctgctcgag gaggcgaaaa agccggcacg
      781 catccccgtt gagccgaaac cccatcttga ggcgcgcttc cgaagcttcg accggcttgc
      841 gaccgccttt gccgtgttcg accgggggca aaggctcacc catttcaacc aggcctatgt
      901 cgcgctgtgg cagctcgctc ccgagtggct tgccacccac ccccgcgacg gtgaaatcct
      961 cgatcgcctt cgccaggcgc ggcggctgcc ggagaaggcc gattatcgcg gctggaagca
     1021 cgattggctt gccgcctacg ggaagaatgt tcaagccgag gatctgtggc acctgcccga
     1081 cggccgcacg ctgcatgtcg tcgccgacag cgagggcgag gccggggtga cctatctcta
     1141 cgagaacgtg accgaacggc tcatgctcga gagccgctac aacgccctga tcgaagtgca
     1201 acgcgaaaca ctcgatacgt tgcgcgaggg cgtcgcggtg ttcgccccga gcggttcttt
     1261 gcggctctac aaccgggcct ttgccgccat ctggcggctg aacccgcgcc agctcgacgg
     1321 cgagcctcat atcgatgaga tcatcgcatg gtgtcgggcg ctttatgatg agcccgaaga
     1381 gtgggagcgg atcaaggcgg cggtgacggc catcgtcgcc gcgcgggtgg cttacgagaa
     1441 gcaattcgac cggaccgacg gcagcgtgct tgcttgcgcc gtgcttccgc ttcccgatgg
     1501 cggcacgctg ttcacctcgg tcgacgtatc ggacgcaaag catgcggagc gcgcgttgaa
     1561 ggagcgcacc gaagctctcg aagcagccga ccgcctcaag accgcgttca tcagccacgt
     1621 gtcgtacgag ctgcgcacgc cgctcaccaa catcatcggt ttcagcgagc tgttggcgag
     1681 cccggccgca ggtcccctca ccgacaggca gcgcgaatat cttggcgaca tccgtctctc
     1741 gggccgcacc cttctttcca tcatcgacga cattctcgat cttgccacca tcgacgcggg
     1801 aacgctcgaa ctcaagcttt cgccggtgaa tgtgagccat gtgatcgagc aggccgcgca
     1861 gggccttgcg gagcggatca agctgaacga tctcggcctt gacatcgaca tcgagccggg
     1921 catcgatgat ttcgtcgccg acggaaagcg tgtcactcaa atgctgtaca atctgctgtc
     1981 aaatgccatc ggcttctccg acacgggcgg aaagatcgcg ctccgctgcg ggcggagaga
     2041 ctcgatgatc gcgttcaccg tcgccgatca gggcgtcggc atccccgagg actatcagca
     2101 ggcggtgttc gatcgcttcg agagccgctc gtacggctcg cgccatcgtg gcgccgggct
     2161 cggactttcg gtcgtcaaga gcctcgccga gcttcatggc ggcacggtca cgctcgactc
     2221 aggcccgggg cgcggcacgc gcgtgacgct tctcctgccc tatcggctcg agccggccgg
     2281 cgagagctcg gagcccgacg cggcgcacta cacgtctcgc gccggatgac cgcaagtgtg
     2341 agtctcgacg atgtcgacct cgcgcggctc gacatcatcg cgggccgtct cgccctcatg
     2401 ctgaaatcgg gggacgtcgt tgctctctcc ggaccgcttg gcgccggcaa gacgactctt
     2461 gcccgcgcgc tgatcgcggg gcttggcggc gcgaccgagg tgccgagccc gaccttcgcg
     2521 ctgatgcagc gctacgagct gccccatctc acgctcaccc attgcgattt ctatcgcctg
     2581 gagtcgcacg agctcgatga gctcggtctc gatgacgcgg taagcgaggg cgtggtgatc
     2641 gtcgagtggc cggagcgcgc cgcaacctgg ctgccgcacg atcgcctcga cgtcgccatc
     2701 gaggagacgg cgaagcccga cgcgcgccgc atcgtgctga cagggcacgg cggctgggaa
     2761 aagcggctcg agcgcctcaa agcgttgtcg gacttcctcg acggcacgcc ttatgccgag
     2821 gcgcccgccc gttacctcct tggcgatgct tcgacgcgat cctacgcgcg gcttgcgctt
     2881 cccgggcgcc cagccattct gatgaactcg ccgcgccaac ccgacgggcc gcccattcgc
     2941 ggcggcaaac cttacagcgc gctcgtgcat cttgccgagg acgtcacgcc cttcgtcgcg
     3001 gtggcgggaa cgctcaaaga ggccggactc tcggcgccca tcgtctacgc gttcgacctc
     3061 gatcgaggat tgatggtgtt ggaggatctc ggcgatgccg tcttcggcag cgaggttcgc
     3121 cgcggccgtg ccttgagcga gctttggggt cccgccgtcg acgttcttgt ggcactcgcg
     3181 gaaaacgagc cgcctcggct cctgcccatt gcgggccatg cgccttaccg gctcccgcgc
     3241 tatgacgcgg gcgcgatgct gattgaggcg tcgcttctca tggattggtt ctggccggcg
     3301 gtccatggga gagccgcgcc gccgtctctc gtaagtgaat tcgaggccct ttggcgcccg
     3361 cttctgtctg acgcggaacg ggacgatccg ggctgggtgc tgcgcgacta tcactcgccg
     3421 aatctgatgt ggcttcccga gcggcaaggc atcgaaagaa ttgggattct cgactttcag
     3481 gacgcgctgc ggggcccatt ggcttacgac cttgtgtccc tgctccagga tgcccgcctc
     3541 gacgtgccgg aagcgcttga acgggagctt ctcgctcgct actgtgcggc tcggggtgtg
     3601 gggagcaagt cgttttcgcg cgatcgtttc gtctcgcttt acgccgttct cggcgcccaa
     3661 cgcaattcta agatcctcgg catctttgcg cgattggcga aacgtgacgg caagcgcggt
     3721 tatctcgctc acatcccgcg ggtcgcgcgc tatctcgagc gcgacctggc gcatccgtcc
     3781 ttggcggaac tgcgccgctt ctacgcacgc gagtttccat cggccgaaaa cctgccgccg
     3841 ctcgtggtgt gagagaggct caccatgaca gaagcgagga cggcgatggt gctggccgcg
     3901 gggctaggcg agcgcatgcg ccccttgacc ttgaccatgc cgaagccgct ggtgcggctt
     3961 gcaggcaagc ctctcatcga tcatgtgctt gaccggttgg ctgacgccgg cgtcaagact
     4021 gccgtggtca atgtccatta cctccccgaa cagctcgaag cgcatcttgc gagccgccgc
     4081 ggccggccgc ccgagacgct tgtctccgac gagcgcggcg ttcttctcga cacgggaggc
     4141 ggaacgagca gggcattgcc tctgctcggc cagggtccct tcttcattca caatgcggat
     4201 tcggtttgga ccgagggcgc ttcgcccgcg ctgtcgcgca tgctgaagaa atggaatccc
     4261 gcgctgatgg atagtctgct cttgctcgcg ccggtcacga ccagcgtcgg ttatgcggcc
     4321 cgcggcgatt tcgccatgag ccaggatggg cggcttgccc gcaggggcgc gaacgaggtc
     4381 gtgccgtttg ccttcgccgg cgtgtcgctc tgcgatgcga cgctgttcaa gaatgctccg
     4441 gaagggcgct actcgctgaa cctcttgtgg gatcgggcgc ttgccaaggg caagctctac
     4501 gggatcaggc tcgacggacg ctggatgcat gtcgggacgc cggcagcctt ggccgaggcg
     4561 gaaaccgcat tcgagcgcga aggtgcctga gacccctcca gagaggcccc ggctctatag
     4621 catcccgcct tcggcgccgt tcctgacaac gcttgccaag gccattctcc aaggcgagct
     4681 cccctcgccc ggcggaccca agcccggtcc gctcactctc ccgctcacca cgatctatct
     4741 cccgacccgc cgcgccgggc gcgcgctgcg cgaggcattc ctcgccgaaa ccgagggcga
     4801 ggccctgctc ttgccgcgca tcagggcgct cggcgatccc gacgaggatg ccgccatcat
     4861 cttcgggacc ggagatttcc cggacggaga cggcgccatg gaggcgcccg ccatcggttc
     4921 cttgcgcagg cggttggcgc tgatgcggct cgtgctcgcc ttcggagagc gcttgggcgc
     4981 ggaggccgcg gccgagagtg gcgacggtgc tgccccgacc ttcgcgttga cgccggggca
     5041 ggcgtcccat cttgccgcag acttggcacg cctcatggat ttcatcgaga gcgaggaggt
     5101 cgcgctttcc gcgctcaagg aaatcgtgcc ggaggaattc gccgggcact ggcagctcac
     5161 cgtcgagttc ctcaagatcg tcaccgagca atggccgcgc tatctcgccg ataacgggct
     5221 catctcgccg gtcgcgcgcc gcacgtatct gatggcggtc gaaaccgagc gcctcctcaa
     5281 gggctcgacc tatccggtca tcgcagcggg atcgaccggc acggtgccgg cgacggcacg
     5341 gctgttgaag gcgatcgcct ctgtcccgaa cggcgcggtc gttctgcccg gtctcgactc
     5401 attcctcgac gacgagacct ggtcgcgcct cgccgatgag ccggagcacc cgcaattcgg
     5461 catggcggaa ttgttgcgca agctcggcgc gacccgcgcc gatgtcgccg acgttcctgg
     5521 agccttgcca gacccggccg gccgagcgcg gctccacctt gcaagcgagg cgttgcgccc
     5581 cgccgatagc accgatcgct gggagcgctt tcttgccggc gacgatcttt ccgccggcgg
     5641 caaggcgagc cttggcaggg cgagcctcgc caacgcgacg tcaggcatcc accttgttgt
     5701 ggcgccgacc gcgcatgacg aggccgaggc gatcgcgctc atgctccgat cggcggtgga
     5761 gacgccaggc aagacggcag ctctcgtcac gccggaccga acgctcgctc ggcgcgtggc
     5821 ggccaggctc aagcgctacg accttgccgt cgacgactcg gccggcatcc cggtcgcgca
     5881 caccgtgccc ggcgcctttc tcgatctcgt actcggcgcg gccgagacga acttcggccc
     5941 gccagagctc atggccctac tcaagcaccc tctggcgctc ctcggacgct ccccgggtga
     6001 ggcgcgatcg gccgcgcggg cgctcgaacg cggcaccttt cgcggcgtct acgtcggaga
     6061 gggtctcgaa ggcgcgcggg cagcgcttca cgccgcgcaa agcgaggagc ggcgcggacc
     6121 ggttgcgctc tcttcctcag agcacgcggg ggcgcttcgt ctggtggcgg atctcgaaac
     6181 cgccttcgcg ccgctcatcg ccttggcagg gccattgccg caacccgcgg ggcggcttgc
     6241 cgaagttcat gcggcggtgg cggaggcctt ggcgcgcgat gcggccggct cgtcatcggc
     6301 gctttggcaa ggcgaagcgg gggaagccct ttcggtgctg cttgccgagt tgatcgatgc
     6361 gggccgcgcc gtcacgctcg acccgcccga ctaccctgcg ttctatcgga gcctcgtcgc
     6421 tggagacgtg gtgcggccgc gcgcgggcct gcatccccgg cttttcatct ggggcccgct
     6481 cgaagcgcgg ttgcagcagc cggagctcgt catcctcggc agcctcaatg aaggggtatg
     6541 gccgcggccc gaggaagcgg gcccctggct cagccggccg atgcgcgcga gcctcgggct
     6601 cccgccgcct gagcgccgta tcggtctctc ggcgcatgac ttcgcccagg cgctcggcgc
     6661 gcgcgaggtc tatctcaccc gagcgctcaa agtcgatggc gtgccgaccg tgccgtcgcg
     6721 ctggctgcag cggctgctcg ctctcgtcaa ggcggcgggc cttgaagcca agctcgcccc
     6781 tgccgagcct tgggtcgcat gggcccgcga gcgcgatacc gctccgccat tcgatcccgt
     6841 gacggcgccg gcgccccgac cgcccttgga agcgcggccg cgcaaattga gcgtgacccg
     6901 catcgaacga tggatcgcaa atccctacga gatcttcgca aaagacatct tgaagctcga
     6961 acccttgaac ccgctcagca ttgagcccga cgccgctttg cgcggccaga tcgtgcatcg
     7021 tgctttgcat gaattctcgc acgcctaccc ggatcgtctc cctgacgata tcgaagaggc
     7081 gcttgtcgcg atcgccgacc ggcatttcgc ggcgcttgcc ggttcgcccg tggtgaaggc
     7141 attttggcgc cccgccctcc gccgcttcgc gcggtggttc gcggcgaccg agcccgcgcg
     7201 ccgatctggg gtgacgaaaa tcgtcaccga ggcttacggc accttcgatc ttgctccttc
     7261 cttccgactc accgcgcgcg ccgatcgcat cgatatcgcc gaggacggat cggcggtgat
     7321 ctacgactac aagaccggca agcctcccct gcaaaaatac gtcgacgaac tgttctcgcc
     7381 gcagctcccc ttggaagcgc tgattgccga ggaaggtggc tttcctgatc tcggcaagcg
     7441 cgccgtcagc ggacttcgct acattcacgt cacgggccgc gccgaaggcg gtgtagagtg
     7501 cgaagcgggc aagaacgctc ccgccgatct cgccagcgag gcgcgagcga agctgacgcg
     7561 gctcatcgcg cgctatgccg acccggccat gccctacgag gtgaagcgca ggtccgcgcc
     7621 ggccttccgc caaatataca agtacgacgc gtatgagcac ctcgcgcgca tcaaggagtg
     7681 gctgacgctg gaggccgagg aggatttcgg atgagttcct gcgcgccgct tccgccggag
     7741 gccgacgcca accaggcgcg cgcgtcagac ccggccgcct cggcctgggt gtcggcaagc
     7801 gccggaaccg gcaagaccga ggtcctggtc aagcgcgcgc tgcggcttct gcttgccggg
     7861 ttcaagcctg aatcgatcct ttgcctggcc tataccaaga ccgccgcggc cgagatgcag
     7921 aaccgcctct tgggcgagct tgccggatgg gcgacagcca aaagcgaggt gttggcctgc
     7981 aagcttaacg agctcacgcg ccaggcgccc gatgcggaga ccttgcgcct cgcgcggcgc
     8041 ctgttcgcgc gcgcgctcga agccaaaggc ggcctcaaga tccataccat ccacggcttc
     8101 tgcgagaggc tgttgcagcg cttcccgctg gaagcgcgcg tcacgccgca tttcaaggtg
     8161 ctcgacgagc gcgagcaggc aagcctgcgg cgggccgctt tcgacgcgac gcttgcccgc
     8221 gccgcagagg agagcgaagc cccgctcggc aaggctctcg ccacggtcat ggccttcacc
     8281 tcgggcgatt atgtccgcca ggtgatcgat acggtgatgg ccaaacgcgc cgcgcttgcg
     8341 cggatcgtcg actatcatgc cgggatcgcg ggttgggccg aggccgaagc cttggcgctc
     8401 aagcggctgt tcggtgtggg cgatgagact gaagagacgc tgctcgcgca gctcgcttct
     8461 gtgctcgacg atgacgcgat ccacgccgcc atcgcggcct ttgcagcttt ttgctcggtg
     8521 gccaggacgg acaaggaggc cgaagcaagc cttcgacagg caagagcaag cgcgggtgac
     8581 agacgcgtcg cggcactcgc ggagatgttc ctcacgcagg atggaaagcc gctaagccag
     8641 ctttgcacca cgcgcttcgc cgcggcggag cccgcgcttg ccgcgagcct caagcaagcc
     8701 caagccgtct tcgccggcct caaggacaag ctcgcgcatc tccgcatggc cgaagcgagc
     8761 gccgccgtcc tcgccatcgc cgacgccgtg cgggacgcgt acgaacggcg caagctcccg
     8821 gacgtcgcgc tcgactatga cgacctgatc gacaaggctc agaacctctt gtcgcgcgcg
     8881 gacgccgcgt cgtgggtgct ctacaagatc gacggcggga tcgaccacat tctggtcgac
     8941 gaggcgcagg acacgaatcc cgcgcaatgg accatcatcg agaatttggc ggcggaattt
     9001 tttgcgggca gcggccggag cgatagactg cgcacgctgt tcgccgtcgg cgacgagaag
     9061 cagtcgatct acagtttcca gggcgcgaac ccgatgcggt tcggcgaggt cggtcgcgcc
     9121 ttccagaaaa gagcaaccgc gctttcgctc gattggcaca ctgtgccgct cacgctgtcc
     9181 tttcgctcga ccgagccgat ccttgatgcg gtggatcgcg tcttcgcgcg ggcgccggcg
     9241 acaaacgggc tgatcttcac cgagggcggc gccgccatca cgcaccaggc gtttcgcaaa
     9301 ggcgaagcgg ggctcgtcga actttggaca atcgaaaccg aggaagaggc cgagcccgcg
     9361 gacgcgttcg agccctggaa cgaggagctg gcgggcgcgc gcgcggtcga cgcgctcgct
     9421 cgacgcatcg ccgcgacgat caagagttgg atcgagcagg gcgaggagct tccggcgcaa
     9481 ggccgcaatg tcagggcggg cgatatcctg atcctggtcc gccgccgcga tccgttcacc
     9541 acgccgatga tccgcgagct gaagcgcctc aatgtgccgg tcgcgggcgc cgaccgcatg
     9601 aagctgaccg atcagatcgc cgtacaagac ttgatcgcgc tcgccgacgt gctgctgatg
     9661 cccgaggacg atcttgctct cgccgtcgtg ctcaagagcc ccctcttcgg cttcgacgac
     9721 aacgacctct tcgcgctcgc ctatcggcgc cccgcatcgc tctggtcggc gctcaaggac
     9781 aaggccaagg atgacacgcg ctttgccgaa gccgtgaaga agctctcctc cttgctcgcg
     9841 cgcgccgact ttctgccgtc ttatgagttc ttcgccgagc tgctcggcgc cgatggacag
     9901 cgcttgcgca agcgcatgct cacgcgcctt gggccagagg ccgccgaggc gatcgatgag
     9961 tttctcgatc gtgccctcgc ctatgaccgg gattccccgc cttcactgca aggcttcgtc
    10021 aacgcgctgc gcgccggcga cgtcgagatc aagcgcgaca tggagcaaca gcgcgacgag
    10081 gtccgcatca tgacggtgca cggcgccaag ggcttgcagg cgccgatcgt gttcctgccc
    10141 gacacctgca cgcgggcgcg cacgcaaggg ccgaggctct atcccttcgc gcgggagggt
    10201 gcgtcggccg aggcggttga ccatctcgtg tggccgccga ccggtcattc gaagctcgcg
    10261 acgctggacg aaagcaagag catcctggag cgcgcggagc gcgacgaata ccaccgcctg
    10321 ctctacgtgg cgatgacacg ggcgcgagac cggctctatg tgtgcggttg gcagggaaag
    10381 cagcagcgcg agaacaactc ctggtacgac ctcatcaagg acgggctcgg ttcgctcgcc
    10441 gagcacgacg cccccggcgg caagaaggtc ggacggatgc attcggcgca gaccaagccg
    10501 gtcgagcgcg tcgaggaaaa acgcgaggca gacgcgacgg cacctttgcc ggaatgggcg
    10561 ttggcgccgg cgccgctcga gcgaacgcgc ccgaagcttg ccccctcgcg gcttgctctt
    10621 ttcggcgagc cgagagcaga gcctttcccc gatcagaccc cgctttcccc acggcgtctc
    10681 ggcgagcgga gccgttttgc caggggacgc cttgtccatg ccctcttaca gcatctgccc
    10741 gagatcgcgc ccggcgagca ggagcgtgtg gcgcgaagct tcgtcgccgc gcgtggcgcc
    10801 gatctttcgc ctgagcttcg agaggagatc gtcgccgaga cccttgccat cgcgcgcgac
    10861 gctctcttcg cgccgctctt ccagccggga agccttgccg aggtgccggt cgtggcgcgg
    10921 atcggaggtt acgatctcga aggccagatc gaccgcctgg ccatggtcga ggacagcctg
    10981 ctcatcgtcg actacaagac caaccggcct ccgccgagga ccttggacga ggtcgctccg
    11041 gcttatatcg cgcagctcgc cggctatcga aaggcgcttg ccgagatgtt tccggggcgg
    11101 cgcttgcgga ccgcgcttct gtggacagac ggtccgacgc ttatggaaat ccccgcaact
    11161 tctctcgatg ccgcggaaga gcggcttttc aacccacccg tcgagccttg acggccttcc
    11221 ggggcatacc taggttccct ccctggcccg agggaaggcc gctcaagccc cggcgatcag
    11281 gagacgcgaa tggcaacgga aaaagtatct gacgacacct tcgaagccga ggtgctgaaa
    11341 tccccgaccc cggtggtcgt cgacttctgg gccgaatggt gcggcccttg ccggcagatt
    11401 gcgcccgctc tcgaagagat cgccagagag ctgaacggca aggtcaagat caccaagctc
    11461 aatatcgacg agaatccacg cacgccgtcc cactacaacg tgcgcgcgat cccgaccttg
    11521 atcctgttca aggacggcga agtcgcggga acgctgaagg gcgcgtaccc gaagaatcac
    11581 ctcgccgctt ggatcaacgg cacggccggg gcctagatcc aagcgcccaa atctaaccgt
    11641 gcagcttgag cacgtggccc gcgagataga gcgagccgca aatcagcacg cgcccggcgc
    11701 ccggcagcgc ggcttgcgac ttcaagagag cgtccggcac gctcgaagcg accgtcacgt
    11761 cgaagcctcc ggcgcgcgcg atgtcggcca aggcctcggc gctgaacgcg ttcggttcgt
    11821 tcgggatcgg cacggtgtag acccgctcga cgagcccctt gaacggggcg atgaaggcgt
    11881 gcgcgtcctt cgtctccatc atgccgaaga cgagatgcat cgtgcgcggc acgcgctcct
    11941 cgagctcggc aagcgcttgc gcgatggccc tgccgccggc aggattgtgg ccgccatcga
    12001 gccagatctc ggtgccgtcg gcgacatagg cgtgaagact gccggcggga aggcgctcca
    12061 gccgcgcccg ccactcggcg tggatcatgc cctgttcgag ggcgcgcgtg gtaagcgtct
    12121 cgccgaatag acgttcagcc gcggcgatcg ccgttcccgc gttgtcgatc tggtggcgcc
    12181 ccttgagacg cggcagcggc agatcgatga ggcgggtcgg cgtgctgaag atgaggcggc
    12241 cgtgctgctc gtacgtgtca aaatcgcggc cggcgacctg gagcggcgcg ccgacctgtt
    12301 cggctcgcgc ctcgatcaca tcaaaggcct cctgctcctg ccggccaacc acgcacacga
    12361 caccgggctt gagaatgccc gccttctccc cggcgatctc cgtgagcgtc tcaccaagaa
    12421 aggagacatg gtcaatcgag acgggcgtga tcgcggtgag cgcggggcga gacacgacat
    12481 tggtggcatc gagccttccg cccaatccgg tttcgagcag caaatagtcg gccggggtct
    12541 cggcgaaggc gaggaaggcg gccgcggtcg tgatctcgaa cagcgtgatc agctcgccgt
    12601 cattggccgc ctcggcacgg gcgagacagt cgacgagcag atcttccgcg atcgggcccg
    12661 agccgtgcgg tccggcgagg acgacacgct catggaaatg gatgagatgc ggcgaagtat
    12721 aaacatgcac ccgcttgccg agcgcctcgg cgatggcccg ggcgaaagcg aggagcgagc
    12781 ccttgccgtt ggtgccggcg acatgcagca ccgggggaag cttgtgttcg ggatggccga
    12841 gcgcggcaag caggcgctcg atacgcccga gcgaaaggtc gatcgattgc ggatgcaacg
    12901 ccttcagccg ctcgagcagg ctatcgctcg aggcgctccg cggacgaggc gaagctcgcc
    12961 cggcgctgtg cggaaattca gtcaatccct cgtgccccta gacgccgcgc ggcggattaa
    13021 cagtcgcgcc atctccggcc gtgtccgtca agtcgtctgc gtcctcttcg tgcgtggcgt
    13081 cgaggccgtt gccggggagc gcgatcgtcg agggcggagc cgcgcgcgag aggttcccgt
    13141 ccttcatctc ggagagcgaa agatcgcgtc cccggtgggt caatagatgg cagaggcggg
    13201 agagcgtccc gcgcagctgg tggcgatgca ccaccatgtc caccatgccg tgttcatgga
    13261 gatattcggc gcgctggaag ccgttcggca gctgctcgcg aatggtttgc tcgatgaccc
    13321 gcggaccggc aaaaccgatc agggcgccgg gctcggcgat atggacgtcg ccgagcatgg
    13381 cataggacgc ggtcacccct cccgtggtcg ggtgcgtcaa gacgacgatg taaggcagcc
    13441 gcgcctcgcg cagttcctgg acggccaccg tcgtgcgcgg catctgcatc agcgagagga
    13501 tgccctcctg catgcgggcg ccgcccgagg ccgcgaacag aatgaacggc gtttcgcgct
    13561 tcaccgcggc acgcatgacg gagatcatcg cttcgcccgc cgccgtgccg agcgaaccgc
    13621 caaggaaatc gaaatcctgt acggcggcaa tgaccgtctg gccttcgagc cggccggcgc
    13681 cggagaggat cgcatcggag agaccggtct tgttgcgcgc gtccttcagc cggtcggtgt
    13741 agcggcgctc atcgcggaac ctgagtggat cgatggggac ttcgggcaga ggcagggttt
    13801 cgtattggcc gttatcgaac agcgatttca acctcgccgt ggccgacatg cgcatgtggt
    13861 agttcgagcc agggaccacg aactggttcg attcgaggtc cttgtaatag atcatctgcc
    13921 ccgtctcggg gcatttgacc cacagattgt ccggcaggtc cttcttgcga aggatgctgc
    13981 ggatcttggg cctgacgacg ctattgatcc agttcacggg tctatcgaac tctcaaaaag
    14041 cttacacgcg ttaaagcact gcggcctcgg ccggggagcg gagcgccgcc gcgaggcgct
    14101 cgacgagccc gagcacttgc gggccagtcg cgcgagtggc gttgccctct tggtcgaggc
    14161 tctgggcgat ggcggtcacg agggccgagc ccaccaccac gccttcggca atcccggcga
    14221 tcgcgctcac ttgtccttcc gtcttgacgc caaagccaac ggcgatcggc aggctcgtct
    14281 gcgccttgat gcgcgcgaca tggcccttga cctcgttcag gtccggcgcc ttcgtcccgg
    14341 tgatgcccgc gatcgaaacg taatagacga atccgctcgt gttggcgagc acggcgggca
    14401 tgcgcctcgc atccgtggtc ggcgtcgcga ggcgaatgaa attgagcccg gccgcagccg
    14461 cggcggggcg aagctcggca tcttcctctg gcggcatgtc aaccacgatc agaccgtccg
    14521 ctcccgcttc ggccgcgtcg gcaatgaagc ggtccaccgg atacacatat atggggttgt
    14581 aatagcccat tagtaccagg ggggtggcgc catcggcggc gcggaaggcc ttcaccattt
    14641 gcagcgtttt tttcattgtc tgcccggcct ttagcgcccg ctgcgaggag gcctggatcg
    14701 ccgggccgtc ggccatggga tcggagaacg gcatgccgag ctcgatgatg tcggcgccgg
    14761 aagccggcag ccccttgaga atggcgagcg aggtctcgta gtcggggtcg ccggcggtga
    14821 cgaacgccac gagccctgcc cgtccttcgc gcttcagcgc cgcaaatctc tcatcgatcc
    14881 gcgtcacgct cacatctcca tgcggaggtg ctcggcgacg gagaaaatgt ccttgtcgcc
    14941 gcgtccgcac atattcatga ccaggagatt gtcgcgcggc aaagtcggcg cgagcttctt
    15001 gacataggcg agcgcgtggg acggctcgag cgcggggatg atcccctcga gccgcgtgca
    15061 gagctggaag gcgtcgagcg cctccttgtc ggtcaccgca acgtaattca cccggccggt
    15121 gtccctgagc caggaatgtt caggcccgac gccgggataa tcgagtcctg ccgagatgga
    15181 gtggccttcc aggatctgac cgtcatcgtt ctggagaagg taggtgcgat tgccgtgcag
    15241 cacgccgggc tcgccaccgc tcatcgatgc ggcatgaccg tttgccacgt cgagcccata
    15301 gccgcccgcc tcgacgccat agatcgcgac cgacggctcg tcgaggaagg gatgaaacag
    15361 tcccatcgcg ttcgagccgc cgccgatgca ggcaacgagc gtgtcgggaa gccgcccctc
    15421 ccgctcctgc atctgctcgc gtgtctcgcg cccgatcacc gactggaaat cgcgcaccat
    15481 ggccggataa ggatgcgggc ccgcgacagt gccgatgatg tagaaggtgt cgtgcacatt
    15541 ggcgacccag tcgcgaagcg cgtcgttcat ggcgtccttc agcgtgccag accctgacgt
    15601 caccggcttc acctcggcgc cgagaagctt catgcgaaac acgttcggcc gctgccgctt
    15661 gatgtcggtc gcgcccatgt aaattacgca aggcagcccg aaacgggcgg cgaccgtcgc
    15721 caccgccacg ccgtgctggc cggcgccggt ctcggcgatg atgcgcgtct tgcccatgcg
    15781 gcgggcgagc tgaatctggc cgaggcagtt gttgatcttg tgcgagcccg tgtggttgag
    15841 ctcgtcgcgc ttgaagtaga tcttggcgcc gccgagttgg gaggtgaggc gctcggcgaa
    15901 atagagcggg ctcggccgcc cggcgtaatg ggtgaggagg tcgacgagct cggcatggaa
    15961 ggcgggatct tccttggcct cggcataggc gcgttcgaga tcgaggatca gcggcatcag
    16021 cgtctcggcg acgtagcgtc cgccatagag gccaaaatgg ccgcgctcgt cgggaccgcc
    16081 gcgataggtg ttgagcgggt tcggctggtc gcgcacgctc atgatgcctt tgcccttggc
    16141 ttagaagaga cggcgccctt tgccgccctg atgaagcgct tcacgagctc cgcatccttc
    16201 tcgccggggc tgcgctcgac acccgacgac acatcgacca actccgcgcc cgtcatgctg
    16261 atggccatgg cgaccgtctc gggggtgaga ccgcccgata gcgcgaaggg cgttccgaaa
    16321 cccttaagaa cgcgccagtc aaaggcgact ccgttaccgc cgggaagagt cgcgccggcg
    16381 tcagctttgg cgtcgaagag aacgcagtcg gcgatggcgg cataggcgcg ggccgcagcc
    16441 acgtcagaag cttcggcgat cgcgactgcc ttcatcaccg gcaaacgcgt gcaggccttg
    16501 atcgcggcca cgcgctcagg cgtttcgccg ccgtgaagct ggagcagatc ggggcgtacg
    16561 gtctcgacca cgtcatcgat caccgcatcg tcggcatcga cgagcacggc gaccgttccc
    16621 acacgcccgc gcgccgctag cgcaagcacc ttcgccgcct gaagcgtcag gttgcgcggg
    16681 ctctttgcga agaacacgaa ccccacgtag tccgcaccgg cttccaccgc cgtctcgacg
    16741 atggcgagcg tccgcacgcc gcagattttg accttagccg gcacgtttgg gctctcgggc
    16801 gcgtttttgg cgatggcgcc accctatgcc aatccagggt gccaagtcta ccgcactgca
    16861 acaattgggc ttgccagccg gttagcggcg cgggcctctg ccaaaagtcc tgtcgcgcca
    16921 gcgccaaacg gcgacccccg cccagatcgc ctcgacgacg ccgaagggcc aggcgccctg
    16981 gaggaaccca tagatcgaag caaggccgca ggcggcggcg aaggcgagga tgaaccaggg
    17041 gctcaggtcc tcgagcgcgt agaagaccag catggcggtg accgcaagca ggccgaaaag
    17101 ggtgagcgcg tccatggaag gctgaaggat caatccacgg cgagacgcgg gcgggcgggc
    17161 gcggatcccg cgccctcgag ctccttttcg agccggtcgg cctgccgacg caattcgcgc
    17221 gcctcgcggg ccccttcccg cgccgtcttc cgccatttgc cctggcggag ccaggtcgca
    17281 atcccgccca cgacgaggcc gagagcaaac gcccccagca ggaacaggaa cagcggcgcc
    17341 tcgagcgaaa ggcctccctc gcttcccccg aaaggatcga gaacgaggag cgcaggcttg
    17401 cgattgacaa tggcgagcgt gaccaggacc acgccaagcg gaatgacaac gagggcgagg
    17461 acgagcttac gccacattga aggctttcac gcgcacccgc taacgggtgc cgccggggtt
    17521 caagcgctcg cgcagttcct tgccggtctt gaagaagggg acgtatttgt cctcgacgaa
    17581 gaccgtgtcc cccgtgcgcg gattgcgccc ctggcgtgcc gcgcgatgct tcaccgtgaa
    17641 ggcgccgaag ccgcgcagtt ccacgcggtc gccgcgggcg agcgcgcccg tgatctcatc
    17701 gagcacgatg ttgatgatgc gctcgacgtc gcgatggaag agatgcggat tggcgccaga
    17761 cactttctga atcaattcag atttgatcat ggaaagcccc actcggccgg gcgccgcgat
    17821 gcggcgcggg ccatacgttc cccgctagtc cgatgcaggg tgccaaaccg aaacaagacc
    17881 gtcaagcttg aggctagcgg agatttgggc tgcgattccg gccaaacgct cgggcaagag
    17941 gccgaccata ggggccaggg actcgaaaaa ccaaccgtag aggcttgagc tttcgacctt
    18001 cggtttccag tcgacgacct tgagccccgg cgggaccttg cgctctttcg tcagccaggt
    18061 cattgccgtt ttctcgtcgc cgatctcgtc ggcgagcttc agctccacgg cctggcgccc
    18121 cgagtagatg cgtccgtcag tgagccccgg gatggatggg gcttcgatgt tgcggcgctt
    18181 gctcacgaga tcgacgaacc agaccctggc ctcctgcacc atttcctggg tcaaggcgcg
    18241 cgccttctcg tcggctggct cgaacgggct cgggatcgcc ttcaagggac cgcttctcac
    18301 ctcctcgacc ttgatgccaa gcgtcttaag gaggtccgtc acctcggccc attggaagac
    18361 gacgccgacc gagcccgtga tggtgttgcc gtaaacgaag atgcggtcgg tggcgatagc
    18421 cgcaatgtag gcggccgagg tggcaagcgt cccgcacacg gcgaccaccg gcttcttctc
    18481 ggcgagacgg cgaatcgagt cgtacatcgc ctcgccgccg gtcgtggtgc cgcccggact
    18541 gtcgatgttg aagatgacgg ccttgacctg atcggatttg ccgaccttgt cgatcagctc
    18601 gttcatcttg cggtcgtcgg tgatgatgcc cgacacggtc acccgggcga cgtggggcaa
    18661 gaagctcccc gaaccgctga cattgcgatc gcccaggaga agcagcccga gagcgatgag
    18721 accgagcgcg acagccgcaa tccgccatgc cgtcacccgc cgcttgagcc ggcggcgatc
    18781 gatcacgctt tcagcgtcga gagccattca tcctcgcgca cggagcctgt tggcaaggga
    18841 cacgcgcctc gtcttattcc tcaggcgcgg tactcggctc gggatcgtcc ttggcgggcc
    18901 gcttgcgctt gggcttgacc ggctcgtcgc cctcgtcgcc tttggccttg ttcagcgccg
    18961 cgccgaggat gtcgccaagc gatgcgccgg agtcggtcga gccgtattgg gcaaccgctt
    19021 ccttttcctc ggcaatctca agcgccttga tcgagacaga gaccttgcgc gacttggcgt
    19081 cgacctgggt cacaacagca tcgaacttct cgccgacgcc gaagcgatcg ggccgttgct
    19141 ccgaccggtc gcgcgacagg tccgatcgcc ggatgaacgt cgtgaggtca tgatcgacaa
    19201 gcttgacctc aagcccgttg tccttgacct ggagcacctc gcacgtcacg cgcatgccct
    19261 tctttagctt ggcggatggc gcgccggcag cgaaaggatc cggggtgagc tgcttgatgc
    19321 cgaggctgat gcgttccttc tcggaatcgg cctcgagcac gaccgccttg gccacatcgc
    19381 ccttcttgta gtccttgagc gcgtcttcgc cggacttgct ccaatcgaga tccgacagat
    19441 gcaccatgcc gtcgatgccg ccgtcgaggc cgataaacag gccgaactcg gtgatgttgc
    19501 ggatctggcc ttcaatcgtc gcgccggggg gatggttggc ggtgaaagcg acccacggat
    19561 tttcctgggt ctgtttcagc ccgagcgaaa tccggcgctt gtcgggatcg acctcaagga
    19621 cctgaacctc gacctcctgg ctcgtcgaga cgatcttgcc gggatggacg ttcttcttgg
    19681 tccagctcat ctccgagacg tggatcagac cctcgacgcc gggctcgagc tcgacgaagg
    19741 caccgtagtc ggtgatgttg gtgaccgtgc ccctgaagcg cgagcctgta ggatacttgg
    19801 cggcgatgcc ctcccacgga tcggcttcca attgcttgat gccgaggctg atgcgctgcg
    19861 tctccggatt gatgcggatg atctgcacct tcaccgtgtc gccgacattc acgatctcgc
    19921 tcggatgatt gacgcgcctc cacgccatgt cggtgacatg gaggagcccg tcgatgccgc
    19981 cgagatcgat gaaggcgccg taatcggtga tgttcttgac cacgccgtcg atgacctggc
    20041 cctcttcgag acgggccacg atctcgcttc gcttctcggc gcgagtttcc tccaacacgg
    20101 aacggcgcga gacgacgatg ttgccccgcc ggcgatccat cttcaggatc tggaagggct
    20161 gcggcacatt catcaaggga ccgacgtcgc ggatcggacg aatgtccacc tggcttcccg
    20221 gaaggaaggc cacggcgccg tcgagatcga cggtgaaccc gcccttgacg cggttgaaga
    20281 tcacgccttc gacccgctcg ccggacttgt atttctcctc gagcctggtc cagctctcct
    20341 cgcggcgcgc cttctcgcgc gacagaaccg cttctccggc cgagttctcg acccgttcga
    20401 gatagacgtc gacttcatcg ccgacggaga gatcgggcga ccggcccgat gtgccgaact
    20461 ctttcagggg aatgcggcct tcggtcttga ggccgacatc gatgaccgcg acgtccttct
    20521 cgattgcgac gatggtgccc ttgaccacac tgccttcctg gagcgcggtt ccggtaagcg
    20581 actcctcaag aagggccgcg aactcgtcgc ggctcggatg gcttacgctc gtctgcggct
    20641 cggtcatcta ggctcctact cggcgtcgca tcatactcgt ctcccgcctc gctagaggct
    20701 cgttcatccc ttgtctttcc cggcgcgaca ggatttgcga aagcttgcgg ggggctgcct
    20761 cctggccgcc ctctcctccg ctcgctaacc ctcgcggaaa tcatcgaaaa atgatgcgcc
    20821 aatgcaaaaa gggcaacggt ctcaattggc ctgcccagca cggcccatcg cagcatcgat
    20881 caaagcgatg gctgccttga acgcggcgtc tatagccaaa ttcgtggtgt cgagcaagta
    20941 cgcgtccctg gcccggcgca agggggcggc ggcgcggctc ttgtcgcgcc ggtcgcgccg
    21001 ggcgatatcg gcaaggacct cggcctcggt catgtcgtgg ccggcaaggc gcaactcgcg
    21061 atagcggcgc tccgcgcgca cttcggcgct cgcggtcacg aacagcttca catcggcatc
    21121 gggacagatg acggtgccga tatcgcggcc gtcgaggacc gcgccggggg ccttgccggc
    21181 gaaggcgcgc tgataggcaa gaagcgcccc tcgaacgtca gcacggctcg caacgatcga
    21241 ggcggcctcg ccgagcttgc catccatcag agtgggatcg tccagcgtgg cggggttaag
    21301 cgccttggcg gcggcgagcg cggcattcgc atcggagagg cttgcccccg ttgccaggac
    21361 gtcgcgcgcg acggcgcggt agagcctgcc ggtgtcgaga taggcgagac cgtaatgggc
    21421 ggcgatgcgc ctgccgagag tgcctttgcc cgaagcggcg ggaccgtcga tggcgatcag
    21481 catgaggcga aattcgctcc gagacgggtc atcaacggca cgaagccggg gaagctcgtc
    21541 ccgatcatgc cgacatcgtc gaccgtgacc ggcgcgcgcg cgccgagccc gagcgtgagg
    21601 aacgccatgg cgatgcggtg atccatcttc gtctcgacgc gagcgccgcc tttgacccgc
    21661 ccctcgcctt gcacaaagag atcgtcgccc tcgacgcgga ccgacacccc gcaagcggca
    21721 aggccttgtt cggtggcggc gagccggtcg ctctccttga ccttcagctc ggcaagcccc
    21781 tccatggccg tctcgccttc ggcgaaggcg gcgaggcagg cgaggatcgg atattcgtcg
    21841 atcatcgacg gcgcccgctc ccgcggcacg cgaacaccct gaagcgcgct tgcgcgcacg
    21901 cggagatcgc ccacaggctc gccgccttcg tcacggcggt cgaggatgtc gatattcgcg
    21961 cccatctcaa gcaacgtctc gatgaagccg gttcgcgtgg gattgaggag cacgccctcg
    22021 acgacaatgt ccgagcccgg agcgatcagc gccgcggcga tcatgaacgc ggccgagctc
    22081 ggatctcccg gcacggcgat gctcgcgccg tggagctcgg cgtctccgcc cacggtcacc
    22141 gcgcgggcgc cctcgccgcg atcctccgcc acgatctcgg cgccgaaaaa gccgagcatg
    22201 cgctcggtgt ggtcgcgcgc ggccaagggc tcgaccaccg ttgtccggcc cggcgcgtga
    22261 aggccggcaa ggagcacagc cgatttcacc tgcgccgacg gcaccggaag gtcgtaaacg
    22321 atgggcaaga gatccgaggt gccgcgcacg acaaggggaa gcgtcgcacg atcggattcg
    22381 gattcggctt gcaggcccat ggcggcaagg ggcgcgagca ctcggcccat gggccgccgc
    22441 cgcaacgacg catcgccggt cagctcgact tgcatatcgt gaccggcgat gacgccgagc
    22501 atgaggcggc tcgcggtgcc ggaattgccg aagtcgagca ccgaagcagg cgcgcgcaag
    22561 ccccccacgc cttgaccctt caccgcgacc ccgccgttca accgctccac ttcggcgccg
    22621 agcgccgaca cggccttcgc cgtcgccgcg acgtcctcgg catcgagcag cccgctgacg
    22681 cgggtcgtgc ccgtagcaag cgcccccagg atcagcgcgc gatgggaaat cgacttgtcg
    22741 cccggcaccc gaatccgccc cgaaaggcgt gcggacgagc gtgcggtcag gggctgcttc
    22801 atagggttct caggcgggga tttcgagggt tcgatagctt ttgacagcac agatgtggcg
    22861 tggcaatggg gaacgcaatc tgaggccatc atcgaggatc atcaatgtcg aaacccgcac
    22921 gggggaccaa gcgcgtttgc ccgagctgtg gagcgcggtt ttacgacttg aaccggacgc
    22981 ccatcacctg tcccgtctgc caggccgtgt accagctgcc gacggcgccg tcgcgccgcg
    23041 gcgaacgcgc atcggcggcc ggagcgcgaa aagtggaaga gcccccgcct cggcccgttg
    23101 aacccgtcga ggtgatcagc atggaggagg tcgaggaggc gggcgccgaa gcgacaatcg
    23161 ccgaggaaga ggaaatcgtc gatctcggcg acgacgctcc ggagattccg ccgggcgacg
    23221 acgacaacgc gttcctcgag gaggaggaag agggcgaggc cgacgtgaca ggcatcgtcg
    23281 gcggcgacaa gggcgaggaa ggctgagggc aggtgtcagg tgtcaggcat cagaaatcag
    23341 aaatcagaag tcagaagtca gaagccagaa ggactcgtgg ctggtgcagg tatgatgtgc
    23401 caggtgccag gtgccaggtg ccggggccgg gttcgactgc cttgttgatt tctgattcct
    23461 gatttctgat atctgatatc tgctttttga tacctgacac ctgacacctg ataggggcca
    23521 tagctcagtt gggagagcgc gtgaatggca ttcacgaggt cggcggttcg atcccgcctg
    23581 gctccaccat ttaaatcaac tacttgcccc tccttttgct gttctctgat tctccaaggt
    23641 aacactagca attggtcaaa gtggccgtat ccgtagcctt gcacgcgccc ggtc
//
LOCUS       DASUQP010000115         4820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_734726,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000115 DASUQP010000000
VERSION     DASUQP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734726"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VFY74_05200"
     CDS             complement(<1..1250)
                     /locus_tag="VFY74_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5931134.1"
                     /translation="MQRFCIIGAGACGLAVAKTFAERGIAFDCFEALSDVGGIWNPGS
                     PHVVYGSTYLNSSKKLSRYPDFEFPEEWAPYASRAQAQDYLRAYAKEFGLYPLIRFNS
                     RVTRAEPAEKGWLVTIEGEKAPRNYDGLIVANGHHWDQYVPTYPGHFTGEMLHSHDVK
                     SKEQLKGKRVLVVGAGNSAVDILSDAALEDSARAIHSMRRTYYFFPKTVFGKPTDVFV
                     DYTSRIPLPRWLMRLLYRYGMRIVVGPHRRFGLERPDHNLFEAHPTACNTYLDHIIHG
                     RITVKPAIERFEGKKVHFVDGSEEEVDFIVWATGFRPSFPFIDSSHILDAQGRSKLFI
                     HTFHRERDDLFVAGLFEPAEGGVWQIADYQARLIASFLLAQAKDPKRVAWFRALKARA
                     SPDIGHGIKWKDTTWHRFEIQHYR"
     gene            1487..2848
                     /locus_tag="VFY74_05205"
     CDS             1487..2848
                     /locus_tag="VFY74_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5931135.1"
                     /translation="MEIKQDVTVKTWDGTWTYHPKVVAVPESAEDLVEIMTDEVRFPS
                     PVRPAGSMHSTARINGDDGGTMVDMKAMNRILHFTGDTVTVEAGATFIVLAQALKERG
                     LQPYVTTEIGNVTMGAMATAASKDSSFPGGFGQVSSYVTGVRLVTPNGRLREINERDN
                     PDEMQIIRSSYGLFGIVYEVTLRVRPTTSLSVRHYSLSLENFRKHFPIYKARGYAVMY
                     YIFPYVKRVLVELRKDNPEAEPNSRYRWVYRNRFWRKYGPAVTLWIQKTTRNRPVRAA
                     ADNLHFFLLRQALVWVVRSDRTWPHAQIINYPRSPGANKYLFSMWAFREGGFFDILEE
                     YCNFCIAYEAKTGYRCDLPSVGYSIARDVEALLSYCWEGATLSIDPASTGGPEWEEFL
                     KVYNQFCSDRRGVPLFNQTPFLTRDQVRKAFGVRIQQFAARRKAVDPKDRMLDSYFRE
                     LLS"
     gene            complement(2918..3688)
                     /locus_tag="VFY74_05210"
     CDS             complement(2918..3688)
                     /locus_tag="VFY74_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5931136.1"
                     /translation="MAEEFPWKLAWITGASGAICGEIARRLASAGVTVAATARPGEKL
                     QAVAAANDRIKAFPADVLEPDALKETVGRIERDLGPIDLALLGAGMYAPFDIRAIDLD
                     GFRRSMALNVNGVINSIAAVLPPMLARNSGNIAIMGSLFGYAGWPGNGSYGASKAAVI
                     NLAESLKLELDGTGVAVTIINPGFVDTPLNASYDPKKKLYVMSKERCARKILERLPRK
                     PYEIAFPPQVELFLKLARSLPRALSFPLIRWFTRRMQG"
     gene            complement(3814..>4820)
                     /locus_tag="VFY74_05215"
     CDS             complement(3814..>4820)
                     /locus_tag="VFY74_05215"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018481713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5931137.1"
                     /translation="NLRIAENATLILPLHRELDSLREAEAGDGRIGTTKRGIGPAYED
                     KVGRRAIRVMDLQDLPSLKPKIERILAHHNPLRRGLGQPEISADELCAQLTEIAPKIL
                     PFVDTVWALLDAKRRQGKRILFEGAQGALLDIDHGTYPFVTSSNTVAAQAATGSGLGP
                     GAINYVLGITKAYTTRVGEGPFPTELKDATGKLIGERGAEFGTVTGRPRRCGWFDACL
                     VRQTRKVVGINGIALTKLDVLDQLPELKICVGYRLDGKELDHLPAAQGAQARVEPVYE
                     TMEGWTESTRGARSWAELPAQCIKYVRRLEELIGAPVALLSTSPKREDTILVHDPFVD
                     "
BASE COUNT          893 a         1521 c         1497 g          909 t
ORIGIN      
        1 aagcggtagt gttgaatttc gaagcggtgc caagtcgtgt ccttccactt gatgccatga
       61 ccgatatcgg gactggcgcg cgcctttagc gcccggaacc aggcgacgcg cttcggatcc
      121 ttggcttgag cgagcaggaa actcgcaatc aagcgcgctt gatagtcggc gatctgccac
      181 acgccgcctt cggccggctc gaacaggcct gcgacgaaca aatcgtctcg ctcgcggtgg
      241 aacgtatgga tgaaaagctt cgacctcccc tgcgcgtcga ggatgtgaga cgaatctata
      301 aaggggaagc tcggcctgaa cccggtcgcc caaacgatga aatcgacctc ctcttcgctt
      361 ccgtccacga aatgcacctt cttcccctcg aatctctcga tcgcgggttt caccgtgatg
      421 cgtccgtgaa tgatgtgatc gagataggtg ttgcaagcgg tgggatgcgc ctcgaacaga
      481 ttgtgatcgg gccgttcgag cccgaatctg cgatgcggcc cgaccacgat ccgcatgccg
      541 taccggtaca gcaggcgcat gagccagcgc ggcagcggga tgcggctcgt atagtcgacg
      601 aacacgtcgg tcggcttgcc gaacaccgtc ttgggaaaga agtagtaggt gcggcgcatc
      661 gaatgaatgg cgcgcgcgct atcctcgagc gcggcgtcag acagaatgtc aaccgccgaa
      721 tttccggcgc ccacgacaag cacgcgcttg cctttcagct gctctttcga tttcacatcg
      781 tgggaatgca gcatctcgcc ggtgaaatga cccggatagg tgggcacgta ctgatcccaa
      841 tggtggccgt tggcgacgat gaggccgtca tagttgcgcg gcgccttctc cccctcgatc
      901 gtcacaagcc agcctttttc ggcgggctcg gcgcgcgtca cgcgcgaatt gaagcggatc
      961 agcggataga gaccgaactc cttggcgtag gcgcgcagat aatcttgcgc ctgtgcccgg
     1021 ctggcatagg gcgcccactc ttcgggaaat tcaaagtcgg ggtagcggga cagcttcttg
     1081 gacgagttga gataggtcga gccatagacg acgtgcgggc ttccgggatt ccagatgccg
     1141 ccgacatcgg agagcgcctc gaagcagtcg aaggctatcc ctcgctcggc gaaggtcttg
     1201 gccacggcaa gcccgcaagc tcccgcaccg atgatacaaa aacgctgcac ggcccattaa
     1261 cctcgcgtgc taaatcttta ggctgccatc cggccacttt gcgccagacc taagcattca
     1321 agccccaggg gttcaaccca ttcggctccc cttctcccgc attgatttgc gtcaattccg
     1381 gggcgagagg acgcggatct gatagcccgc gggctgcgcc gcttctctcc tggattcgtt
     1441 ttcgctttgc tttaaggtca gccgccaaag ggcgccgggg aacctcatgg agatcaagca
     1501 ggacgtcacc gtcaagacct gggacggcac atggacttac catcccaagg tcgtggccgt
     1561 gccggagagt gcggaagact tggtcgagat catgaccgac gaggtgcgct ttccctctcc
     1621 ggtgcgcccc gccggctcca tgcattccac cgcccgcatc aacggcgacg atggcggcac
     1681 catggtcgac atgaaggcca tgaaccgcat cctgcacttc accggcgata ccgtgacggt
     1741 cgaagccgga gcgaccttta tcgtgctcgc acaagccctg aaagagcggg ggctccagcc
     1801 ttacgtcacg acagagatcg gcaatgtcac catgggcgcg atggcgaccg ccgccagcaa
     1861 ggacagctcc ttccccggcg gcttcggtca ggtcagctcc tacgtcacgg gggtgcgact
     1921 cgtcacgccc aatggccgct tgcgcgaaat caacgagcgc gacaatcccg atgagatgca
     1981 aatcattcgt tcgagctacg gactgttcgg catcgtctac gaggtgaccc tcagggtcag
     2041 gcccaccacg tctctctcgg tgcgtcacta ctcgctcagc ctggaaaatt tccgcaagca
     2101 tttcccgatc tacaaggcgc gcggctacgc cgtgatgtat tacatcttcc cttatgtgaa
     2161 acgcgtgctg gtcgagctgc gcaaggacaa tcccgaggcc gagccgaatt cgcgctaccg
     2221 ctgggtctat cgcaaccgct tctggcgcaa atacgggccc gccgtcacgc tgtggattca
     2281 aaagaccacg cgcaaccgcc cggtgcgggc ggccgccgac aacctgcact tcttcctgtt
     2341 gcgtcaggcg ctcgtatggg tggtgcgcag cgaccgcacc tggccgcacg cgcaaatcat
     2401 caactatccg cgctcgcccg gcgccaacaa atatctgttc agcatgtggg ccttccgcga
     2461 gggcggcttt ttcgacatcc tcgaggagta ttgcaacttc tgcatcgcct atgaagcgaa
     2521 gaccggctac cgctgcgatc tcccgagcgt cggctattcc attgcccgcg atgtcgaggc
     2581 cctgctctcc tattgctggg agggggcgac gctcagcatc gaccccgcct cgacgggcgg
     2641 gcccgagtgg gaggagttcc tgaaggtcta caatcaattc tgcagcgaca ggcgcggcgt
     2701 gccgctgttc aatcagacgc cgttcctgac ccgcgatcag gtgcgcaagg cctttggcgt
     2761 gaggatccag caattcgccg cgcgccggaa agccgttgat ccgaaggatc gcatgctcga
     2821 ttcctatttc cgcgagctgc tgagctagtg cttcggcgag ctttgtctgg atacagcacc
     2881 ttttcatccc cccttgatgg ggaggtcagg agaggagcta cccctgcatc ctgcgagtga
     2941 accagcggat cagcgggaaa gagagagcgc gcggcaagga gcgcgcgagc ttcaggaaca
     3001 gctcgacctg gggcggaaag gcgatctcgt aaggcttgcg cgggagccgc tcgagaatct
     3061 tccgcgcgca gcgctccttg ctcatcacgt agagcttctt cttcgggtcg taagaggcgt
     3121 tgagcggggt gtcgacgaag cccggattga tgatcgtcac ggcgacgccg gttccgtcca
     3181 attcgagctt aagactttcg gcgagattga tcaccgccgc cttgctcgcg ccatagctgc
     3241 cattgccggg ccacccggcg tagccgaaca gcgagcccat gatggcgatg ttgccgctgt
     3301 tccgtgccag catcggcggg agcacggcgg caatgctatt gatgacgcca ttgacattga
     3361 gcgccatcga gcggcgaaag ccgtcgagat cgatcgcacg gatgtcgaaa ggcgcgtaca
     3421 tgccggcgcc caacaaggcg agatcgatcg gcccaaggtc gcgctcgatc ctgcccaccg
     3481 tctccttcag cgcgtcgggc tcgagcacgt cggcggggaa agccttgatg cggtcattgg
     3541 cggcggcgac cgcctgaagc ttctcgcccg gccgcgccgt tgcggcgacc gtcacgccgg
     3601 cggaagcaag gcgccttgcg atctcgccgc agatcgcgcc cgaggcgccg gtgatccacg
     3661 cgagtttcca gggaaattct tccgccaaat ttcacctcac cgttgtcatg cccgggcgaa
     3721 gtcccgggca tctacgaatt ctgttcgagc agcaaaaacg tggatggccg ggacaagccc
     3781 ggccatgacg aaaaaggtca ggtcaaccat acctcaatcc acgaagggat cgtgcacgag
     3841 gatggtgtcc tcgcgcttcg ggctggtcga gagcagcgcg acaggcgcgc cgatcagctc
     3901 ctcgagccgc cgcacatatt tgatgcattg ggccggaagc tcggcccagg agcgcgcccc
     3961 gcgtgtcgat tccgtccagc cttccatcgt ctcgtagacc ggctcgacgc gggcttgcgc
     4021 cccttgcgcc gcaggcagat ggtcgagctc ttttccgtcg agcctgtatc ccacgcaaat
     4081 cttgagctcc ggcagctggt cgagcacgtc gagcttggtg agcgcaatgc cgttgatgcc
     4141 gaccactttg cgcgtctggc gcacgaggca cgcatcgaac cagccgcagc gtcgcgggcg
     4201 cccggtgacc gtgccgaact cggccccgcg ctcgccgatg agcttgcccg tcgcgtcctt
     4261 gagctcggtc ggaaacggcc cctcgcctac ccgcgtcgtg taggccttgg tgatgccgag
     4321 cacgtagttt atcgcgccag ggccgaggcc cgagccggtc gcggcctgag ccgcgaccgt
     4381 gttcgacgac gtaacgaagg gataggtccc gtgatcgatg tcgagcagag cgccttgggc
     4441 gccctcgaag aggatgcgct tgccctgccg ccgtttggcg tcgagcaacg cccataccgt
     4501 atcgacaaag gggaggatct tgggcgcgat ttcggtgagc tgcgcgcaga gttcgtctgc
     4561 ggaaatttca ggctggccca agccccttcg gagcggattg tgatgggcga ggatgcgctc
     4621 gatcttgggc ttgagcgacg ggaggtcctg gaggtccatc accctgatgg cgcgccgccc
     4681 gaccttgtcc tcgtaggccg ggccgatgcc gcgcttggtc gtgcctatgc gcccgtctcc
     4741 cgcctcggcc tcgcgtagcg aatcgagctc gcggtggagc ggcaggatga gcgtcgcatt
     4801 ctcggcgatg cggagattgt
//
LOCUS       DASUQP010000116         6822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1007771, whole genome shotgun sequence.
ACCESSION   DASUQP010000116 DASUQP010000000
VERSION     DASUQP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007771"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1252..1503)
                     /locus_tag="VFY74_05220"
     CDS             complement(1252..1503)
                     /locus_tag="VFY74_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931138.1"
                     /translation="MAKKQQARASGFVLFDVSYEDGTQTSNRKVPAADLGGLDGDLPA
                     RTFVEAQDQEIGAMSGRPRGRIKSITRSRAGSRVPARNN"
     gene            complement(1517..1678)
                     /locus_tag="VFY74_05225"
     CDS             complement(1517..1678)
                     /locus_tag="VFY74_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931139.1"
                     /translation="MPFKPNYNQQRADRNRSKAQKKQEKLKRLEEETARRKAARGEAP
                     GPDKTQEDA"
     gene            complement(1706..1951)
                     /locus_tag="VFY74_05230"
     CDS             complement(1706..1951)
                     /locus_tag="VFY74_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931140.1"
                     /translation="MSKFSSGGSSARRNAEALLNKTQKREASFKLEQAQEHEAMMLKT
                     ARLRELRLAKEEEDKRLQEAAPLPPKRPAKPRRSRQR"
     gene            complement(1948..3252)
                     /locus_tag="VFY74_05235"
     CDS             complement(1948..3252)
                     /locus_tag="VFY74_05235"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5931141.1"
                     /translation="MTSPSFDGLGLIEPLRRALKAENYLQPTPIQARTIPLLAAGNDL
                     LGIAETGTGKTAAFALPILQLLAKQRSPAESQPRLTGAPRALILAPTRELAGQIGERF
                     RAYGRGLPLHTAVIFGGVNQHHQVKALKAGIDILIATPGRLLDLCRQRHVRLDHVSIL
                     VLDEADRMLDMGFIHDMRKIVSLCPRDRQTLLFSATMPAPIAKLAHDILDRPVRVDIA
                     PAKVAVERIEQRVFFVGAKEKRALLEALLADQAMDRVLVFTRTKRGADRVCRNLVQAG
                     FAATALHGNKAQNARVRALDSFRSGRVPILVATDIAARGIDVPNISHVVNYELPNEPE
                     SYVHRIGRTARAGAGGAALSFCHHGERPYLQAIERLMRSTVAVADHRLASVAPVSHRQ
                     SGRGTTSASTKPHRRSRGRRFAGVRHRAPAPANGAGAIGELT"
     gene            complement(3500..3994)
                     /locus_tag="VFY74_05240"
     CDS             complement(3500..3994)
                     /locus_tag="VFY74_05240"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5931142.1"
                     /translation="MNDSANPQIAAEAPREANRNAAALFENFMLCAPVTKAMHALAEQ
                     HVVEIRRAYERSKTALETSMATVERTFDAVGKGAAALNLKVMDIAQRNIDTGFDLAKS
                     LAGARDLAEIVELQAAYWRKQFDVLGAQAEEVRSLSAQVSADATRSVWQPVARSQEEL
                     RKAS"
     gene            4702..6447
                     /locus_tag="VFY74_05245"
     CDS             4702..6447
                     /locus_tag="VFY74_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5931143.1"
                     /translation="MIKPSHYDDDGYPIPWVRSAIPSNTLACLFALAEDARRRQILGP
                     GVDLRLHTYDETNRRVRPGRIMRMIRKEGGKALIGLVGVQSNQFPRAVDLARPFLAAG
                     LPVCIGGFHVSGCISMLPEMPPEMREAQALGISFFAGEAEEKRLDQVLQDAWKGKLAP
                     LYNHMKDLPALAGEPPPILPRKHVRRTAGSLSSIDLGRGCPYQCSFCTIINVQGRKSR
                     FRSADDLEQLVRQNVAQGIHRFFITDDNFARNRDWEKLVDRLIELRGEGLNIGFTIQV
                     DTLCHRIPNFIEKTTAAGVRRVFIGLENINPDNLLAAKKRQNKITEYRYMLQKWREHG
                     ATTYAGYILGFPGDTKESILHDIEIIKRELPLDVLEFFFLTPLPGSEDHKVLLEKGAW
                     MDSDLNKYDLDHRVSHHSRMSDAEWEEAYRAAWLAYYTPEHVRAILRRVAANKRGRPA
                     TTLSTILWFYLMIAFEGVHPLEGGAFRMKFRRDRRHGLPRENPFAFYPRYLGEIVRKA
                     WGYWSVYRQHKKILNEVLEAQDRWSYSDLAIAASQEEELDTLELFKETRGGDAAVAKK
                     RKEDWARESLRAALP"
     gene            complement(6497..>6822)
                     /locus_tag="VFY74_05250"
     CDS             complement(6497..>6822)
                     /locus_tag="VFY74_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931144.1"
                     /translation="LANSLIIGALAISPAALWERQWPALAMLSAGLLVSAAWLGIATY
                     GWSAMQRHVEIAGTFASRYFEHLPNPFGETIYNRAQARMHHLILLVIAVFMFVYIGLG
                     FVRLS"
BASE COUNT         1203 a         2113 c         2204 g         1302 t
ORIGIN      
        1 tgaagggccg cgttccgctt tgcttaagcc ttggttgcgg cctctccggc gtcgctcacc
       61 gtggcgggcc aaccgtccag gctgcggctc tcggatcgcg gccgccgaac caccggaaca
      121 tcttccgctc gcgacgctcc actttcttgg tcaatccgtc acgccgcgca tctgacagcg
      181 tgacctgggg ttcgttcgct gcctgctgca cgggcttggt cgcctggcgc gccgatcgcg
      241 ctttttgaga cgccgttttg gagaccgctt tgggaggcct gggcgcggcg cttgcttgcg
      301 ttacgggttg agtggggctc ggtccatcga tcgctgccag atacttttca aagacaaagc
      361 cgcgctcttg cgtcaccggg tcctggagct cgagccaacc gctttcgcga ccgacgatct
      421 gcaactccgt gccggtggga tagaacctcg tgatggggga cgaaacggac gccgccctgt
      481 gcgccttcgc gccgaggatc agcctcgccc acgtgacatg ttccgctgcg gcaggggtcg
      541 atcgcgccgg ggcttgcgct gcgggagcca cgccggcgtc aggtggttgc tccgaagctt
      601 gatttgcgcc ggcgcgcccc ggccaatagg cggcctgttg ctggggtggg gaggacgtgt
      661 cggagtgtcg agagacggcc gctagacttg cgctgtcccg gcctagagac tggagggttg
      721 agccccaaga agaacgcagc tttcgctcgc cttgaggaat gggcttaggc ttcgcggtcg
      781 cgaccttcgc gcccgtaccc cattccggct cttcatttgt gggaatcaag gcgtaagccg
      841 cggcgccggc aaggcatacg caaatgagca accgcatcat ggccaggtcc tcctccctgg
      901 cccccgtctc tatgttgtgt tccgcgcagc ggatttcaat ctacgccctc ggcaaggatc
      961 agacaagcat ctggcttggg cgtcgcgtct cgcggtaatg aaatcctcaa cgtcgttggg
     1021 ggcccgcaat tatgacgcgg cgaagcgaga cgaggttcaa tctccggcga tcgtggccgg
     1081 ggcggtcggg gtaccgctga cggggcatgg atcccatccg ccgcgctttc cggcgggtcg
     1141 cactggcgca gcagtcctcg gggctcccat ccgtagtgat tgtcgctcgg gcgaagccca
     1201 acaactggat tgagcgccag cgggacctcg ccaaggccct cggacgtggg atcagttgtt
     1261 tctggcgggt acccgcgatc cggcccgtga gcgcgtgatc gacttgatgc ggccgcgcgg
     1321 ccgtcccgac atcgcgccga tctcctgatc ctgggcttcg acgaaggtgc gggccggtag
     1381 gtcgccgtcg agaccgccga gatcggcggc cggaaccttg cggttcgagg tctgcgtgcc
     1441 gtcctcatag gagacgtcaa aaagcacaaa gccggaagcg cgggcttgct gtttcttggc
     1501 catggttctg cttcgcctag gcgtcctctt gcgtcttgtc cggacccggc gcttcgccgc
     1561 gcgccgcctt gcgcctcgcc gtctcctctt cgagacgttt cagcttctcc tgcttctttt
     1621 gcgccttgga ccggttgcgg tccgcgcgct gctggttgta gttcggcttg aacggcatgt
     1681 tctcaactcc gtgtcgggga attccctagc gctgccgcga ccggcgcggc ttggcgggcc
     1741 gcttcggcgg tagcggtgcc gcttcctgca acctcttgtc ttcctcttcc ttggcgagac
     1801 gaagctcccg caacctcgcc gttttcaaca tcatggcctc atgctcctga gcttgctcga
     1861 gcttgaagct tgcctcacgc ttttgagtct tgttcagaag agcctccgcg ttgcgccgtg
     1921 ccgaggagcc gcctgatgaa aatttgctca tgtcaattct ccaatggcgc ccgcgccgtt
     1981 cgcaggcgcc ggggcgcgat gccgaacgcc ggcaaagcgc cggccacgcg agcgtctgtg
     2041 cggcttggta gaagcggatg tcgtgccgcg tccggattgg cgatgagaca caggtgcaac
     2101 gctggcaaga cgatggtccg cgacggcgac ggtcgaacgc attaaccgct cgatcgcctg
     2161 aaggtaaggc cgttcgccgt ggtggcaaaa ggagagcgcc gcgccgcccg ctcccgcgcg
     2221 cgcggtgcgg ccgatgcggt gcacgtagct ttccggctcg ttgggcagct cgtagttgac
     2281 cacatgcgag atgttgggga catcgatgcc gcgggcggca atatcggtgg caaccagaat
     2341 gggtacgcga ccgctgcgga acgaatcaag cgcccgcacg cgcgcgttct gcgccttgtt
     2401 gccgtggagt gccgtggcgg caaagccggc ctggacgaga ttgcggcaca cacgatccgc
     2461 gccgcgcttg gtgcgggtga agacgagcac ccggtccatg gcttgatcgg cgagcagagc
     2521 ttcgagcagc gcccgcttct ccttggcgcc gacgaagaac acgcgctgct cgatccgctc
     2581 gaccgcgacc ttggccgggg cgatgtcgac gcggacgggg cggtcgagaa tgtcatgtgc
     2641 gagcttggcg atgggcgcgg gcatggtggc ggagaacagc agcgtctgcc ggtcgcgcgg
     2701 acacagcgag acgatcttgc gcatgtcgtg gatgaatccc atgtcgagca tgcggtcggc
     2761 ctcgtcgaga acgaggatcg agacatggtc gagccggaca tgacgctgcc ggcagaggtc
     2821 gagcaggcgt ccgggcgtgg cgatcagaat atcgatcccg gctttcagcg cctttacctg
     2881 gtgatgctgg ttgacgccgc caaagatcac cgccgtgtgc aaggggagcc cgcgtccata
     2941 ggcgcggaag cgctccccga tctgaccggc gagctcgcgg gtgggcgcca gaatgagcgc
     3001 gcgcggtgcg ccggtgagcc gcggctggct ctcagcgggg gatcgctgct tggcgagaag
     3061 ctgcaggatt ggcagagcga aggctgccgt cttgcccgtt cccgtctcgg cgatgccgag
     3121 cagatcgttt cccgcggcga gcagcggaat ggtccgcgcc tggatcggcg tcggttgcaa
     3181 atagttttcg gccttcagcg cgcgccgtaa cggctcgatc aggccgaggc cgtcgaaact
     3241 gggtgatgtc acgaaatacc tcaggcgccg ccaccggacg ggcgcggatg cgcaagccgg
     3301 caacggcgtt tgccttaccc cacgcgctat ccggggccag acgatgaaag gaaattgtct
     3361 cgggatgaga caccgcactc tgcggcagct tcgcgcgatc gcgagcaatc aatgtgagcg
     3421 gaacatggcg acaacgcgcc gacatgtcaa gtctttgccg gtcttggcca aacaagactt
     3481 gggcctatga cgcgtgccgt cagctcgctt tgcgcaattc ttcttggctc cgcgccaccg
     3541 gctgccaaac cgaccgggtc gcatcggcgc taacctgcgc cgagagcgag cgcacctcct
     3601 cggcctgagc gccgaggaca tcgaactgct tgcgccaata ggctgcctgc aattcgacaa
     3661 tctcggcgag gtcccgcgct ccagccaggc tcttggcaag atcgaagccg gtatcgatgt
     3721 tgcgttgggc aatatccatg accttgagat tgagtgcggc agcgcccttg ccgaccgcgt
     3781 cgaacgtgcg ctccacagtc gccatcgagg tttcgagcgc cgtctttgag cgctcatagg
     3841 ctcggcgaat ctcaaccaca tgttgctcgg cgagcgcatg catggccttg gtgacgggag
     3901 cgcaaagcat gaaattttcg aacagggcgg ccgcgttgcg atttgcctcg cgcggggctt
     3961 cggcggcgat ttggggattt gcggaatcgt tcatagtgca cctcttttgg gggatagggc
     4021 gtcgggagcc tttcgcgggt aaggtaagca gcccgaagcc atagggctca gggttcgtgt
     4081 cgcggcgccg atgttctcgc cgctctgtca gcgcggaaag ctctcaagag cccattcgcg
     4141 aagctcaact ggatcggtac gccatcggaa gccagcacct taagcctccg gctcagcgct
     4201 gctctcagct ccgaatcccg ttccaaatca ggaaacgggt cgcgcgcctg tagcatcacc
     4261 caagtcgctt caaatgcctc tttcaaggca tcaggcgtct tggggtcgta aggatcgatt
     4321 gagcggctta gagagtacat ttctccccct ccttgcttct gcaggcggga gtcttaggct
     4381 ctcagccgtc ggtgcctgtt attgctctaa ccggcgattg tcgaaccgtg cgcctttcct
     4441 tggcgaatag cgagtaaaaa aagctttgcc ttgggtccca cggcagcgcc tcaattgagc
     4501 cgattgatcg acgccaaggc ttgcaattga ggctgaagag gcgcagcttg gccggaaggg
     4561 gggaggggtc atttctgacg ctcccgtatg cacgaactca gcccgtccgc agcggtcgca
     4621 ggagggcaga aatccccaac gccggccgaa tataaagggg tgacggtgaa ggcgacgaga
     4681 gtggaaccgt ttcatttcgt catgatcaag ccgtcgcatt acgacgacga cggctacccc
     4741 attccgtggg tgcgatcggc tattccctcc aacacgctcg cgtgtctttt tgcccttgcc
     4801 gaggatgcgc ggcgccgtca aatcctcggg cccggcgtcg acctccgcct ccacacctat
     4861 gacgagacca accggcgcgt ccgccccggc cggatcatgc gcatgatccg gaaagagggc
     4921 gggaaagcgc tgatcgggct ggtcggcgtg caatcgaacc agtttccacg cgccgtcgat
     4981 ctggcccggc cgttcctggc cgccggtctt ccggtctgca tcggcggctt tcacgtgtca
     5041 ggttgcattt cgatgctgcc ggagatgccg cccgagatgc gggaagccca ggcgcttggg
     5101 atttcctttt ttgccggcga ggccgaggaa aagcggctcg accaggtgct gcaagatgcc
     5161 tggaagggga agctcgcgcc cctttacaac cacatgaaag acttgcccgc acttgccggc
     5221 gagcccccgc ccattctgcc gcgcaagcat gtgcgccgca ccgccgggtc gctgtcgagc
     5281 atcgatctcg ggcgcggctg tccctatcaa tgctccttct gcaccatcat caacgtgcag
     5341 ggacgcaaga gccggttccg ctcggcggac gatctggagc agctggtgcg ccagaacgtc
     5401 gcgcaaggaa tccatcgttt cttcatcacc gatgacaatt ttgcccgcaa ccgcgattgg
     5461 gagaagctgg tcgaccgctt gatcgaactc aggggcgagg ggctgaatat cggctttacc
     5521 atccaggtcg acacgctctg ccaccgcatt cccaatttca tcgagaagac gaccgcggcg
     5581 ggggtgcggc gggtgtttat cgggctcgag aacatcaacc ccgacaatct gctggcggcg
     5641 aaaaagcggc agaacaagat caccgaatat cgctacatgc tccagaaatg gcgcgagcac
     5701 ggcgccacga cctatgccgg ttacattctc ggctttcccg gcgacaccaa ggaatcgatt
     5761 ctgcacgaca tcgaaatcat caagcgggaa ctgccgctcg atgtgctcga attcttcttc
     5821 ctcacaccgc tgcccggctc ggaggaccac aaggttctgc tggagaaggg cgcgtggatg
     5881 gattccgatc tcaacaaata cgacctcgat catcgcgtca gccatcactc gagaatgtcg
     5941 gatgcggaat gggaggaggc ttaccgcgcg gcgtggcttg cctattacac gcccgagcat
     6001 gtgcgcgcga tcctgcgccg ggtcgccgcc aacaagcggg ggaggccggc aacgacgctt
     6061 tcgaccatcc tctggttcta tctgatgatc gccttcgagg gcgtccaccc gctcgagggt
     6121 ggcgcgttcc gcatgaagtt tcgacgcgac cggcgccacg gcctgccgcg cgaaaatccg
     6181 tttgccttct atccgcgcta tctcggcgag atcgtgcgca aggcgtgggg ctattggtcg
     6241 gtctaccgcc agcacaagaa gatcttgaac gaagtccttg aggcgcagga tcgctggagc
     6301 tacagcgatc tcgccatcgc tgcctcgcaa gaggaggagc tcgatacgct cgagctcttt
     6361 aaggagacgc ggggcggcga cgcagcggtc gcgaaaaagc gcaaggagga ctgggcgcgc
     6421 gagagcctcc gcgccgccct gccataatat caaagaggca ccggtggatg cggtagctcc
     6481 tcggcagcat gcaggctcaa gagaggcgca cgaagccgag cccgatatac acgaacataa
     6541 agacggcaat cacgagcagg atcagatggt gcatccgcgc ttgcgcgcgg ttgtagatgg
     6601 tttcaccgaa cggattcggc aggtgttcaa agtagcgcga agcaaaggtc ccggcaattt
     6661 ctacatgacg ttgcatggcc gaccatccat aggtcgcgat cccgagccat gcggcgctga
     6721 caaggagccc ggcacttagc atggcgagcg caggccattg cctttcccaa agcgccgcgg
     6781 ggctgatggc aagcgcgccg ataatcagcg aattcgccag ga
//
LOCUS       DASUQP010000117         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_736180,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000117 DASUQP010000000
VERSION     DASUQP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736180"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..762
                     /gene="recR"
                     /locus_tag="VFY74_05255"
     CDS             157..762
                     /gene="recR"
                     /locus_tag="VFY74_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5931145.1"
                     /translation="MAKVAAGPEIERLIQLLARLPGLGPRSARRAALHLVKKKEQLLE
                     PLATALAEARDTILTCETCGNIDTKSPCTLCRDPARDRALICVVEEVGDLWALERAGV
                     LNGLYHVLGGTLSPLDGMGPDDLTIAGLIARARDANVKEVLLALNATVEGQSTAHYLM
                     DQLTCCNVTVSRLAHGVPIGGELDYLDEGTLAAAVKSRKPM"
     gene            complement(778..2118)
                     /locus_tag="VFY74_05260"
     CDS             complement(778..2118)
                     /locus_tag="VFY74_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEX5931146.1"
                     /translation="MTVTGEARQQPAQRSWTEILFTRKMLTCIFLGFSSGMPLYVLIS
                     LVPAWLRSNGVDLATIGLFALVGLPYTWKFLWSPLMDRFKLPFLGRRRGWAVLVQLGL
                     LVSIGVLGQFDPTSSLQLIVAVVFIVAVFGASQDIVIDAYRRELLADDELGTGTSIWV
                     NAYRLSGLVPGSLALILADYLPWTVVFWVTALFMLVGIVTTLMIKEVSDDKLAPRTLR
                     EAVVNPFVEFFSRDGIMAGLAILAFLFLYKLGDNMATALATPFYLDMGYSLSEIGSVA
                     KAAGLWASIAGGTLGGIIMLKVSINRALWLFGFVQMFTILPFVWLSQAGHTLAGLFVV
                     VSGEYLGIGLGAIALTAFMARETSKAFTATQFALFSSFIAVPRTLANASTGFIVEAVG
                     WTQFFVLCTLAAIPGMLLLFKVAPWSERPPRKQTRRLTEIEKKEQLEQLDADPG"
     gene            2296..2754
                     /locus_tag="VFY74_05265"
     CDS             2296..2754
                     /locus_tag="VFY74_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5931147.1"
                     /translation="MVRVVGIDHLVLSVGDFKASKAFYAPLMAFLGFKVEAEYRGMMG
                     WHNDKTLFWIAAADANGKKHKYRKGDIGFHHYAFRLRSRKDVDALEAFLEAQGATVVD
                     PAGEYYDDYYAVFFLDPDGMKLEGMRYGEHHEQAARKRAARKKTQGRKRN"
BASE COUNT          581 a          909 c          884 g          461 t
ORIGIN      
        1 gtcattccgg cgaaagccgg aatccagaag cggcatgcac cgcgcttgta accctggata
       61 ccggcttgcg cgggcatgac agattggtgt gcggtggcat tgttttcaaa gcaccgcctc
      121 tccgctcccc gcttgcaagg agatgaggct ctagccatgg cgaaagtcgc ggcaggacct
      181 gagatcgagc ggctgatcca gctgctcgcg aggctgccgg ggctcggccc ccgttcggcg
      241 cggcgcgcgg cgttgcatct cgtcaaaaag aaggagcagc tgctcgaacc gctcgccaca
      301 gcgctcgccg aggcccgcga caccatcctc acctgcgaga cttgcggcaa tatcgacaca
      361 aagagtccat gcacgctctg ccgcgatccg gcgcgcgacc gcgcgctcat ctgcgtggtc
      421 gaggaggtgg gcgatttgtg ggcgctcgag cgcgcgggcg tgctgaacgg gctctatcac
      481 gtgctcggcg gcacgctctc gccgctcgac ggcatgggcc ccgacgatct caccatcgcc
      541 ggactgatcg cacgcgcgcg cgatgcgaac gtgaaagagg tgctgctcgc gctcaacgcc
      601 acggtcgagg gacaatcgac ggcgcattat ctgatggacc agctcacctg ctgcaacgtc
      661 accgtgtcgc ggctcgccca cggcgtaccg atcggcggcg agctcgacta tctcgacgaa
      721 ggcacgctgg ccgccgccgt caaatcccgc aaaccgatgt aggtctcgaa ccgccaatca
      781 tcccggatcg gcgtcgagct gctcgagctg ctctttcttt tcaatctcag taaggcgccg
      841 cgtttgtttt cggggcggcc tctccgacca gggcgccacc ttgaacaaca acagcatgcc
      901 gggaatcgcg gcgagggtgc agaggacgaa gaactgtgtc caccccacgg cttccacgat
      961 gaaaccggtc gacgcattgg cgagcgtgcg cggaaccgcg atgaaactgc tgaacaaggc
     1021 aaattgggtc gccgtgaagg ccttgctggt ttccctggcc atgaaggccg tcagcgcgat
     1081 cgcgccaaga ccgattccca ggtactcccc gctgaccacg acgaacagcc ccgcgagcgt
     1141 atggcctgcc tggctcagcc atacgaacgg aaggatggtg aacatctgca cgaaaccgaa
     1201 gagccacagc gcccggttga tgctcacctt gagcatgatg atgccgccga gcgtgccgcc
     1261 ggcgatgctg gcccacaggc cggccgcctt ggccacggac ccgatctcgc tcaagctgta
     1321 gcccatatcc aggtagaagg gcgtcgccag cgccgtggcc atgttgtctc ccaacttgta
     1381 gagaaacaaa aaggccagga tggccagccc cgccatgatt ccgtcgcggc tgaaaaactc
     1441 cacgaaggga ttgacgacgg cctcccgcaa ggtgcgcggc gccaacttgt catcgctcac
     1501 ttccttgatc atcaaagtgg tcacgatgcc gaccagcatg aacagggcgg tgacccaaaa
     1561 caccaccgtc cagggcaggt agtcggccag gatcaatgcg agagaacccg gcaccaggcc
     1621 ggaaagccgg taggcattga cccaaatgga cgtgccggtt cccagttcgt cgtcggccag
     1681 cagctcacgc cgataggcat cgataacgat gtcctggctc gctccgaaca cggcgacaat
     1741 gaacacgacc gcgacgatca actggagcga gctggttgga tcgaactgtc ccaacacgcc
     1801 gatcgaaacg agcaacccta gctgcaccag gaccgcccag ccccgccggc ggccgaggaa
     1861 aggcagcttg aagcggtcca tcaaaggcga ccacagaaat ttccaggtgt agggcaggcc
     1921 aaccagggcg aacaggccga tcgtcgcaag gtccacaccg ttgctgcgca accaggcggg
     1981 caccagcgag atcaggacat agagcggcat tccggagctg aaccccagga aaatgcaggt
     2041 cagcatcttc cgggtgaaca ggatctcggt ccaactgcgc tgcgcaggtt gctgtcgtgc
     2101 ttcccccgtg acagtcatcg cctctgtgat agcaggctcg cgcccccgcg tctcccctct
     2161 ccccttgggg gagaggggtc caatcggcaa aaccccctca ctcggcgaaa gcctcgctat
     2221 gctcgccttt cgccaccctc tcccgcgagg ggagaggggt ttggcccagg cggcatggca
     2281 ataaaggaga gcgtgatggt gcgcgtggtt gggatcgatc acctggtact cagcgtcggc
     2341 gacttcaaag cgtcgaaagc cttttacgcg ccgctcatgg cgtttctcgg cttcaaggtc
     2401 gaggccgagt atcggggcat gatgggctgg cacaacgaca agacgctgtt ctggatcgcc
     2461 gcggccgacg cgaacggcaa gaagcacaaa taccgcaagg gcgatattgg ctttcaccac
     2521 tacgcgtttc gcttgagaag ccggaaggac gtcgatgcgc ttgaggcgtt tctcgaggcg
     2581 caaggtgcga ccgtcgtcga cccggcgggc gaatattacg acgactatta cgccgtcttc
     2641 ttcctcgatc ccgacggcat gaagctcgag ggcatgcgct acggcgagca ccacgagcag
     2701 gcggcgcgca agcgcgcggc aaggaagaag acgcagggtc gcaagagaaa ttaacctccc
     2761 cctcgtgggg aggtcggcgc gccgcttcgt ttgcgctacc gcgccgctgc ttgagcgcaa
     2821 ggcgcgccgg gtggg
//
LOCUS       DASUQP010000118         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_600626,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000118 DASUQP010000000
VERSION     DASUQP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600626"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..1946)
                     /locus_tag="VFY74_05270"
     CDS             complement(390..1946)
                     /locus_tag="VFY74_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX5931148.1"
                     /translation="MRIARAAIALAAPLLILAVPMPARAEQEPSQGELRAAKALDDIR
                     GDPLALYDFLKRLPKGADLHSHLHSAVFAETLIEDAIDDKLCVDVEARAFAKPQGAGD
                     GAPSCAEGTVPAATIYKNQQLYDALIDSFSMRGFVPSEGDTGHDHFFGAFKKFGGTDP
                     SHTGEWLDEVANRAARENTQYLELMATPTWNLLNSITKDVAWREDLGGLRRELLAKGL
                     AAEIPGARAFWDAAEAKRRERGRCGEQGEAAGCTVALRYIYEVFRNNPKELVFAQALF
                     GFELASADPRVVAINFVGEEDGHDAMTDYAEHMRMVGFLRTLYPNVHVSLHAGELAPG
                     LVPPEGLCCHIRLAVDQAGTDRIGHGVDAMYEDRPYALLKTMADKGVLVEINLTSNDM
                     ILGVSGNRHPFATYRKLGVPVALSTDDAGIERIDLTHEYVRAVADYALAYAELKDIVR
                     NSLEYSFLPGAGVWAGKGDYSRLGDDCLNDVPGGGEPSPRCASLLAGSEKAAQQWELE
                     RRFQQFEASF"
     gene            complement(2005..2427)
                     /gene="ndk"
                     /locus_tag="VFY74_05275"
     CDS             complement(2005..2427)
                     /gene="ndk"
                     /locus_tag="VFY74_05275"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5931149.1"
                     /translation="MAIERTLSIIKPDATARNITGQIISRLEGAGLRVIAQKRVWWTK
                     KDARKFYDIHKEQPFFKDLISFMTSGPIVLQVLEGENAIARNREVMGATNPVDAAPGS
                     IRKDFALNMQRNSVHGSDSAATARREIALCFKKSEIVG"
     gene            complement(2520..2984)
                     /locus_tag="VFY74_05280"
     CDS             complement(2520..2984)
                     /locus_tag="VFY74_05280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX5931150.1"
                     /translation="MGEGSATEVHFYQLERKTLDDVLPNLLERSLERGWRAAVQAASE
                     ERVAALDTLLWTYRQDSFLPHGTARDGRASAQPIYLTAGEDNPNAAHVRFLVDGATLA
                     DTSPYVRVAYIFDGRDQAAVSHAREAWQDAKARGDAVKYWQQDAEGRWQLQA"
     gene            complement(2977..>3140)
                     /locus_tag="VFY74_05285"
     CDS             complement(2977..>3140)
                     /locus_tag="VFY74_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5931151.1"
                     /translation="TFVLFCSRPKALPDSYARYLVNSLRDNFDMPGVPIRLNLRKGDN
                     PFVRGSRDG"
BASE COUNT          535 a         1018 c         1016 g          571 t
ORIGIN      
        1 gcttcgcgcg gcgggcggaa agccctctcc cacgagggca gaggggaaaa atcgggcgct
       61 cctcagcatg agggggttgg tttgttcccc gtcatggccg gcggagggcc gggcatgacg
      121 aggcattttg aacgacctca ccctgaggag ggccgaaggc ccgtctcgaa gggtgggact
      181 ctttcttccg ctctcccctt ggggagagca cgcgagcgca tgttcgcgct cgcggtgagg
      241 ggtaagccgc gaagcggcgt cgaagaaaga gccccctcac ccttcccgcc attcgccgcg
      301 ctccgcgcgt cgggcgagaa gccctctccc acgaggggag aggggaaaaa tcgagcgctc
      361 ctccgcgcgc gcctcggctt ctcttccact cagaagctcg cttcgaactg ctgaaagcgc
      421 cgttccagct cccattgttg cgccgccttc tcgctgcccg cgagcaagga cgcgcagcgc
      481 ggcgacggct cgcccccgcc cggcacgtcg ttcaggcagt cgtcgccgag gcgcgaatag
      541 tcgcccttgc cggcccacac tcccgcgccg ggcaggaaac tgtattcgag gctgttgcgc
      601 acgatgtcct tcagctcggc ataggcaagc gcatagtccg cgacggcgcg gacatattcg
      661 tgggtcaggt cgatgcgctc gatgccggcg tcgtcggtcg agagcgcaac cggcacgccg
      721 agcttgcgat aggtggcgaa cgggtgccga ttgccgctga cgccgagaat catgtcgttg
      781 ctggtgaggt tgatctcgac gagcacgccc ttgtcggcca tggtcttcaa caacgcgtaa
      841 ggacggtcct cgtacatcgc gtcgacgccg tgaccgatgc ggtcggtccc ggcctggtcc
      901 accgcaagcc tgatgtggca gcacagccct tccggcggca ccagcccggg agcaagctcg
      961 ccggcgtgaa ggctgacatg aacgttcgga tagagcgtgc gcaagaaccc gaccatgcgc
     1021 atatgctccg catagtcggt catggcgtcg tgcccatcct cctcgccgac aaaattgatg
     1081 gccacgacgc gcgggtcggc tgaagcgagc tcgaagccga acagggcttg cgcgaagacc
     1141 agctctttcg gattgttgcg gaagacctcg tagatgtagc ggagcgccac ggtgcagccg
     1201 gcggcttcgc cttgttcgcc gcagcgcccg cgctcccggc gtttggcctc ggccgcgtcc
     1261 cagaacgcgc gcgcgccggg aatctcggcg gcaagaccct tggcgagcag ctcgcgccgc
     1321 aggcccccca ggtcctcacg ccacgccaca tccttggtga tggagttgag gaggttccag
     1381 gtcggcgtcg ccatcaattc cagatactgg gtgttctcgc gggcggcgcg gttcgccacc
     1441 tcgtcgagcc attcgccggt atggctcgga tcggtgccgc cgaatttctt gaacgcgccg
     1501 aagaaatggt catgcccggt gtctccttca gaaggcacga acccgcgcat cgaaaaggaa
     1561 tcgatcaagg cgtcgtaaag ctgctggttc ttgtaaatgg tcgcggcggg aaccgtgccc
     1621 tccgcgcagg acggcgcgcc gtcgccggcc ccttgcggct tggcgaaggc gcgcgcctcc
     1681 acgtcgacgc agagcttgtc gtcgatggcg tcctcaatca gcgtctcggc gaacacggcg
     1741 ctgtgcaaat gactgtggag atcggcgccc ttcggcaggc gcttgaggaa atcgtagagc
     1801 gcgagcggat cgccgcgaat gtcgtcgagc gccttggcgg cgcgcagctc gccttggctc
     1861 ggctcctgct cggcgcgcgc cggcatcggc acggccaaga tcaagagagg ggcggcaagc
     1921 gcgatggcgg ctcgtgcgat cctcatggcc gtttctagca tgaagcccgc ctcgtccgcc
     1981 tccagggctt cgcgaccggc gcccctaccc cacgatttcg ctcttcttga agcagagcgc
     2041 gatctcgcgc ctggccgtcg cggcgctgtc ggagccatgc accgagttcc gttgcatgtt
     2101 cagggcgaaa tccttgcgga tcgagcctgg cgccgcatcg acgggattgg tcgcccccat
     2161 cacctcgcga tttctggcga tggcgttctc gccttcgagc acctgcagaa cgataggccc
     2221 cgacgtcatg aagctgatga ggtctttgaa aaagggctgc tccttatgga tgtcgtagaa
     2281 tttcctggcg tcctttttcg tccaccacac gcgtttttgc gcgatcacgc gaaggcccgc
     2341 gccttcgagc cgggatatga tctgcccggt aatgttccgc gcggtggcat cgggtttgat
     2401 gatcgaaagc gtgcgctcaa tggccatacg aaaaagtccc tcttatgtct tggaatctgc
     2461 ggcttatagc ggccgggcat cacagcgaca aggtgaatgg cgtgacgttt tgatgaacct
     2521 catgcctgca gctgccagcg cccttcggca tcttgctgcc aatacttgac ggcatcgcct
     2581 cgcgccttgg cgtcctgcca ggcctcccgc gcgtgcgaga cggcagcctg gtcgcggccg
     2641 tcgaaaatat aggcgaccct cacatagggc gaggtatcgg cgagcgtcgc gccatcgacc
     2701 aggaagcgca catgcgcggc gttcggattg tcctcgcccg cggtgagata aatgggctgg
     2761 gccgaggcgc gaccgtcgcg tgccgtgcca tgggggagaa agctgtcctg ccgatatgtc
     2821 cataacagcg tgtcgagcgc cgcgacgcgc tcttcgctcg cagcctgaac cgcggcgcgc
     2881 cagccgcgct cgagcgagcg ttccagcaag ttcggcagca cgtcatcgag cgtcttccgc
     2941 tccagctgat agaaatgcac ttccgtggcg cttccctcac ccatccctgg accctcgcac
     3001 gaacggatta tcgcctttgc gcaagttcag cctgatcggc acgcccggca tgtcgaagtt
     3061 gtcgcgcaag gaattgacca ggtagcgcgc ataagagtcg ggcagcgcct ttgggcgcga
     3121 gcagaaaaga acgaaggtcg
//
LOCUS       DASUQP010000119        13207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1011263, whole genome shotgun sequence.
ACCESSION   DASUQP010000119 DASUQP010000000
VERSION     DASUQP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13207
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011263"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFY74_05290"
     CDS             <1..685
                     /locus_tag="VFY74_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX5931152.1"
                     /translation="SLAGQGSIAVSDGAIEGVNLTEMIDQVGAGEIPDLRQGPGAKTA
                     FSDLGGSFTVKDGIAETNNLTATSPLMKVTASGTVNIVESRMDLLAKPEIVAGPEGER
                     GANDLAGLSVPVRIEGPLDRPTIRPEVKEMFADPEKAGKTVNQIGKAIQKKFKGKPVG
                     EAIGRFLGDIKIEQRGNGENGGSEAQADRRRSGKKETQQATPDAEAGAAEQDESAEPE
                     DPEIEDLLR"
     gene            complement(852..1700)
                     /locus_tag="VFY74_05295"
     CDS             complement(852..1700)
                     /locus_tag="VFY74_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5931153.1"
                     /translation="MADASHPSPSAEAGKPKTALVFAGGGSFGAVQVGMLRALVAHGL
                     KADMVVGSSVGAMNGAYFAGGLRRRDVFPVTARALLGLIRQRDFLLGSHGLFRLVHTH
                     LPFRNLEEARIPLHVVATDLLSGQPVVLSRGDAATAIVASTAIPAAFAPVKSGNLYLA
                     DGAITSCTPVKIAVMTGATRLIVLPAGYACARAMPPQGAMANALHALTLLISRQVKAE
                     LESLDQTIDFHVAPPLCPLTGSPYDFSRTDELIERGAKRTEEWLAEGGLERRKIPHEM
                     NAHKHH"
     gene            1810..3201
                     /gene="rimO"
                     /locus_tag="VFY74_05300"
     CDS             1810..3201
                     /gene="rimO"
                     /locus_tag="VFY74_05300"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX5931154.1"
                     /translation="MDDIPAKATAPRIGFVSLGCPKALVDSERIITRLRAEGYELSAS
                     YEGADAVVVNTCGFLNSAKEESLAAIGEALAANGRVIVTGCLGVEAERIREAHPQVLA
                     ITGPQQYEAVVAEVHKALPRPHDPFLDLVPPQGIRLTPRHYAYVKISEGCNNRCSFCI
                     IPSLRGRLKSRDLADVMREAERLVAAGVQELLVISQDTSAYGLDLGYAKSVWRDEAYE
                     TRFLALAQALGSLGVWVRLHYVYPYPHVDKVLPLMADGRVLPYLDIPFQHASPAILKA
                     MRRPAHREKTLERIARWRAICPDLAIRSTFIVGFPGESEDDFAELLDWLAEAKLSRVG
                     CFKYEDVAGAAANALPGQVPEEIKAERYDRLMRHQQAISAALLSSRVGKTIEVMVGRL
                     DADGAIARSHWDAPEIDGNVYVNGGQNLKPGDRLSVLVDDADEYDLHARPHRATAAAP
                     RRHGAARDVGAPV"
     gene            3554..4060
                     /locus_tag="VFY74_05305"
     CDS             3554..4060
                     /locus_tag="VFY74_05305"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX5931155.1"
                     /translation="MIKDLIILTIFPGAMALAAVTDLFTLTVPNRLVLALAALFFLAA
                     PVAGLGLHDIGIHVVLALAALGLGFILFSLGVVGGGDAKLFAASLLWIGPEAIVPFAV
                     YAALIGGALALLLLFWRSLPLPATLASQGWLVRLHSPEQGVPYGIALAAAGLLAYPAT
                     RFMAALGG"
     gene            4198..5502
                     /locus_tag="VFY74_05310"
     CDS             4198..5502
                     /locus_tag="VFY74_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtpF protein"
                     /protein_id="HEX5931156.1"
                     /translation="MIGDAAFSLGSAPPSEPVPDRESEVFKASRLRSLPRVNIQAFCE
                     DQDTAASIEKAAQDRRLARARVNVQMGGLDAACAFYRNAATPNLIIVETLLERARMLA
                     ELDRLAELCDPATKVLIVGHINDVFLNRELVRRGVSEYLVAPVGVLQIIESLSSIYSE
                     PDAKPAGQTIAFVGAKGGAGSSTVCHNTAFAIASALEREVVIADFDLPFGTAGLDFNQ
                     DPLKGIADALASPGRLDAHTLDRLLSRCSDQLSLFAAPATLDFAYDLDPRSCETVLDV
                     LRESAPWIAVDVPHLWTAWARDVVMRADHVVITAAPDIANLRNAKNLIDQIKTARPND
                     RLPTLVLNQVGLHKRPEVAIKDFGDAVALDPTVIIEFDAQLFGTAANNGQMIEEVSHK
                     SRAGEAFRKLAGILTDRSEQSAERPSILWSMLTRLGLARTGR"
     gene            complement(5522..6211)
                     /locus_tag="VFY74_05315"
     CDS             complement(5522..6211)
                     /locus_tag="VFY74_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5931157.1"
                     /translation="MTMAEGFSMTANGYTLVIGDKNLSSWSLRPWLALKQCGIHFAEA
                     RIRLRQPDSKAEILRHTPSGKVPALNTKWGVVWDSLAILEFLAERHPEYAFWPRKEEP
                     RAAARSISAEMHAGFATLRHEMPMDLLGRMPSPPIDAVLESEIRRVVAIWKDTRARFG
                     AGGPFLFGAFSNADAMYAPVATRFRTYGVDLARYGDDGTAARYAAALLALPAMAEWTE
                     GAEAETRSRTA"
     gene            6273..7700
                     /locus_tag="VFY74_05320"
     CDS             6273..7700
                     /locus_tag="VFY74_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX5931158.1"
                     /translation="MTSKATTLEETRRGLEDLLLPPGSEAIPVHFIASSEDPALEGLS
                     AAERSWVEAQDWNAKQGSVLILPDAHGGIGGVLLGTGGGDGAAQSPLLTGVLATSVPE
                     GDYRLASPPAGAELAVLGFLAGSYAFTRYKSASADKKKRLVLPEGVDRDGVLALAEAL
                     YFGRDLINTPANDMGPAELESAARELATAFGAGIKVTEGSGLLSDNFPMIHAVGRASD
                     RSPRLIDLRWGAERAPKVTIVGKGICFDTGGLDIKPSSAMALMKKDMGGAASALALAL
                     LVMRAKLPVRLRVLIPAAENSISGNAFRPGDVLPSRNGMTVEIGNTDAEGRLVLADAI
                     ALADEEAPDYLVTVATLTGAARIALGPDLPPLYTDDEEFAARLLASGLAVGDPLWRMP
                     FWAPYDKQLKSAVADVSHIAEGPFAGSIIAALFLRRFVRNAKRFAHLDIFGWVPSEQP
                     GRPKGGEPQGARALFHFFREELEGS"
     gene            7697..8590
                     /locus_tag="VFY74_05325"
     CDS             7697..8590
                     /locus_tag="VFY74_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5931159.1"
                     /translation="MIDTVLPDPRRHAYRGDLASEALRGRIEARRFVKGEPRQVVAPS
                     LPLRREPRFDATLDTEALRGETLTVYDESEGWAWVQLSRDGYVGYMPSEGLTRRMIAP
                     THRISALRTYVYPEPDAKTPPLALLSMNAGITAQRDEGKFLALGGGGYVAAIHAAPIG
                     APSPDFVAVAEAFLGTPYLWGGRTSIGLDCSGLVQLALEAAGHAAPRDADMQAQELGA
                     AIDWRPGAALRRGDFVFWEGHVGIMTSPADFLHANAFHMAVAAEPFAHARDRIKAAGY
                     EMTGVRRLAEVAAAGGARRGP"
     gene            complement(8719..10353)
                     /locus_tag="VFY74_05330"
     CDS             complement(8719..10353)
                     /locus_tag="VFY74_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5931160.1"
                     /translation="MSGNGPLISVEDLSVDFRAGDDVTHAVRRVSFDIGKAETVALVG
                     ESGSGKTVTALSILRLLPYPAASHPSGRIRFNGEDLLTLPSSALRAVRGNKISMIFQE
                     PMTSLNPLHTIEQQVGEVLKIHRRMSDKTARVRVLDLLDKVGIDDPKGRLQSYPHQLS
                     GGQRQRVMIAMALANEPDLLIADEPTTALDVTIQAQILDLLLKLKAEFHMAMLLITHD
                     LGIVRKMADRVCVMNSGEIVERGTTRDIFAAPKHPYTKHLIASEPKGSPPAADGTAKT
                     ILKTKDLRVWFPIRRGFLRQTVGHIKAVDGIDLEVKEGQTLGVVGESGSGKTTLGLAV
                     LRLVSSEGPIVYLGDRIDGYDSKRMRPLRRHMQIVFQDPYGSLSPRLSVAQIIEEGLA
                     IQNPGLTRAERDQRVARALKEVGLDPAFRDRYPHEFSGGQRQRIAIARALVLEPKFLI
                     LDEPTSALDVSVQAQIVDLLRELQRRHKLAYLFISHDLKVVRALANSIIVLRHGKVVE
                     QGPARTVFTSPKTDYTKALLAASFELEATRSGAVAT"
     gene            complement(10397..10615)
                     /locus_tag="VFY74_05335"
     CDS             complement(10397..10615)
                     /locus_tag="VFY74_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931161.1"
                     /translation="MNKIVKEHYPATKLPVELRERIDPAGHVTVTVVEEEKPPEHVMT
                     LEEIIAARRPPYLTATEIDEEIRDLRGR"
     gene            complement(10628..11482)
                     /locus_tag="VFY74_05340"
     CDS             complement(10628..11482)
                     /locus_tag="VFY74_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5931162.1"
                     /translation="MGLAAGLTGAFMFAEIVGGVVAGSLALLADAGHMLTDFASLALA
                     WFGFRLARRPADWKRTYGFDRFQVLVAFANGLALFAIAAWVVYEAIERLTTTPEVSGG
                     IMVVVAGLGLLVNIAAFALLQGADRENLNIRSAAIHVLGDLLGSVAALIAGAVILITG
                     WTPIDPLLSILVAAIIVRSGWRVVADAGHILLEGAPEELDTRAIGPDLIANVKGVEAV
                     HHVHVWSITQSRRMVTLHALICETEDSDRMVRSIKARLKERFGLDHATVETERGTCAD
                     ADPAKAKA"
     gene            complement(11566..12729)
                     /locus_tag="VFY74_05345"
     CDS             complement(11566..12729)
                     /locus_tag="VFY74_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5931163.1"
                     /translation="MDAPTDTAIVEEVTAQGLPRARPWLSVLQRRRWEVFKSNRRGFW
                     SLWIFLALFVISLFAEFIANDKPILVSYKGGLYMPIFRAYPETTFGGIFETEADYRDP
                     AVKAMIADGGGWTLWPPIRFSYNTQNKNPPMAFPVTPTWLLTDADCKLAVERGFHPCN
                     AVLEWNWLGTDDQGRDVVARAIYGFRISVLFGLVLTVFSSLIGVAAGAVQGYFGGWTD
                     LLFQRFIEIWTSVPQLYLLIIVAAVIEPNFWILLGILLAFSWVALVGVVRAEFLRARN
                     FEYVTAARALGLSNLRIIFKHLLPNAMVATLTFMPFILNGSITTLTSLDFLGFGLPPG
                     SASLGELLAQGKDNLQAPWLGLTAFFVIAIMLSLLIFVGEAVRDALDPRKTLT"
     gene            complement(12733..>13207)
                     /locus_tag="VFY74_05350"
     CDS             complement(12733..>13207)
                     /locus_tag="VFY74_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5931164.1"
                     /translation="DNWWELPWYLKIADYFWHLVLPLIAMALSAFATTTFLTKNSFLE
                     EIRKQYVMTARAKGLPERHVLYGHVFRNAMLIVIAGFPGAFIGAFFAGSLLIETIFSL
                     DGLGLLGFESIVNRDYPVVFATVYIFSLLGLLINLLSDLTYTWVDPRIDFETREV"
BASE COUNT         2360 a         4284 c         4326 g         2237 t
ORIGIN      
        1 ctctctcgcg ggccagggca gcattgcggt gagcgacggc gccatcgaag gcgtcaacct
       61 gaccgagatg atcgatcaag tcggcgcggg cgagatcccg gacctgcgcc agggaccggg
      121 cgccaagacc gcgttcagcg atctcggcgg cagtttcacc gtcaaggacg gaattgccga
      181 gaccaataat ctgacggcga cgagcccgct tatgaaggtc acggcctcag gcacggtgaa
      241 catcgtcgaa agcaggatgg acctcctcgc caaacccgag atcgtggcgg gacccgaggg
      301 agagcggggc gccaacgatc ttgccgggct ctcggtgccg gtgcgcatcg aggggccgct
      361 cgaccggccc accatcaggc cggaggtcaa ggagatgttc gccgatcccg aaaaggcggg
      421 aaagacggtg aaccagatcg gcaaggcgat tcagaagaag ttcaagggaa aaccggtcgg
      481 cgaggcgatc ggccggtttc tcggcgacat caagatcgaa cagcggggga acggcgagaa
      541 cggcgggagc gaggctcaag ccgatcgccg gcgctccggc aagaaagaga cgcagcaagc
      601 aacgcccgac gccgaggccg gcgcggccga gcaggacgag agcgcggagc ctgaagatcc
      661 cgagatcgaa gacttgctgc gctagagcat ggtccaatcg cattgaacca tgctgccgac
      721 cccaccccgg ccctcccact tacggggggg ggagagcgat acgccgttcc caattttttc
      781 tcctgaaagc ggggagtaag atgggggtca tgattcaatt cgatgggaca atgctctagg
      841 gcgcccgccc gtcaatgatg cttgtgggcg ttcatctcgt gagggatctt gcgccgttcg
      901 agcccgccct cggcgagcca ttcctccgtc cgtttggcgc ccctctcgat caattcgtcc
      961 gtgcgcgaga agtcgtacgg cgagccggtc aacgggcaga gcggcggcgc gacatggaag
     1021 tcgatcgtct ggtcaaggct ttccagctcg gccttgacct gccgggagat cagcagcgtc
     1081 agcgcatgga gcgcgttggc catggcgcct tgcggcggca tcgcgcgcgc gcaggcatag
     1141 cccgccggca gcacgatcag ccgcgtcgct cctgtcatga ccgcgatctt gaccggcgtg
     1201 cagctcgtaa tggcgccgtc ggcgagatag agatttccgg actttaccgg cgcgaaagcc
     1261 gcagggatgg cggtgctcgc cacgatcgcg gtcgcggcgt ctcccctcga tagcaccacg
     1321 ggttggccgg agagaaggtc cgtggcgacg acatgcaagg ggatgcgcgc ctcctccaga
     1381 ttgcggaagg gcagatgcgt atggaccagg cggaaaagcc cgtgcgagcc gagcaggaag
     1441 tcgcgctggc gaatgaggcc gagcagcgct ctcgccgtaa cgggaaaaac gtcgcggcgc
     1501 cgcaagcctc cggcgaaata ggcgccgttc atggcgccca cgctcgaccc caccaccatg
     1561 tcggctttca agccatgcgc cacgagcgcc cgcagcatgc cgacctggac ggcgccgaag
     1621 ctgccgcctc cggcaaacac gagcgccgtc ttcggcttgc ccgcttcggc cgagggggac
     1681 ggatgtgaag cgtccgccat tctgcccaac cccaactgat ggagcccata agaggcctat
     1741 ccgctttcgg cctgaatcga aactttccgc cggtcgacgg aaccgccccg ttcgcctata
     1801 agcgcgccaa tggacgacat cccggccaaa gcgacggcgc ccagaatcgg ctttgtgagc
     1861 ctcggctgcc ccaaggccct ggtcgattcc gagcgcatca tcacgcggct tcgcgccgaa
     1921 ggttacgagc tgtccgcgag ctatgagggc gccgacgcgg tcgtggtcaa cacctgcggc
     1981 ttcctcaaca gcgccaagga ggaatcgctt gcggcgatcg gcgaggcttt ggcggcgaac
     2041 ggccgcgtca tcgtgacggg ctgcctcggc gtcgaggccg agcgcattcg cgaggctcac
     2101 ccgcaagtgc tcgccatcac cggcccgcag caatatgagg ccgtggtcgc cgaggttcat
     2161 aaagcgctgc cgcgtccgca cgatccgttc ctcgatctcg tgccgccgca ggggatcagg
     2221 ctcacgcccc gccactacgc ctatgtgaag atctcggagg gctgcaacaa caggtgcagc
     2281 ttctgcatca tcccgagctt gcgcgggcgg ctcaagagcc gcgaccttgc cgatgtcatg
     2341 cgcgaggccg agcgtctcgt tgccgcgggc gtgcaggagc ttctcgtcat ctcgcaagac
     2401 acgagcgcct atgggctcga tctcggttac gccaagagcg tctggcggga tgaggcgtac
     2461 gagacccgct tcctcgctct cgcccaagcg cttggttcgc tcggggtctg ggtgcgcctt
     2521 cactatgtct atccttatcc gcatgtggac aaggtgctgc cattgatggc cgacgggcgc
     2581 gtgctgccct atctcgacat tccgttccag catgcgagcc ccgccatcct gaaggcgatg
     2641 cgccgcccgg cgcatcggga gaagaccttg gagcgcatcg cacgctggcg cgcgatctgt
     2701 cccgaccttg ccatccgctc gaccttcatc gtcggcttcc ccggcgagag cgaggacgat
     2761 tttgcagagc ttctcgattg gctcgccgag gcgaagctct cccgcgtggg ctgcttcaaa
     2821 tatgaagacg tcgccggcgc cgccgccaac gcgcttcccg gtcaagtccc cgaggagatc
     2881 aaggccgagc gctacgatcg cctcatgcgg catcagcagg cgatcagcgc ggcgctgctc
     2941 tcatcccgcg tcggcaagac tatcgaggtc atggtcggtc gcctcgacgc tgacggcgcg
     3001 atcgcccgct cgcattggga cgcgcccgaa atcgacggga atgtctatgt caatggcggt
     3061 caaaacttga aaccggggga ccgcctgagc gtgctggtcg acgacgccga cgaatatgac
     3121 ctccatgccc ggcctcatcg cgccacggcg gcggcacctc ggcggcacgg ggccgcacgc
     3181 gacgtgggcg ccccggtttg agtgccttca cccgcctgcg gcccgaagcc ttcggcggac
     3241 gggccggagc gaagcccggc ttcgcgcaga caggtccgct gcttcagcgc aaagcccccc
     3301 acccggcgcg cctcgcgctg aagcaacgga gcggtacgca aaatgtgcca gcgcgccgac
     3361 ctccccacga gggggaggtt agaaaaaaac caaggcgcgt gtagtgtgca tgtgagtctg
     3421 aataccggct tccccgcctt cgcgtcgacg aaggcaggca agccgggcaa tgacaatgac
     3481 agagaattaa cccggccttc agcgcgcctc cgtgacattc gcccatcacg aaacaggcag
     3541 ctaaaaccgg cggatgatca aggatctcat tattctgacg atctttcccg gcgccatggc
     3601 actcgccgcg gtcaccgatc tcttcaccct tacggttccg aaccggctcg ttcttgcgct
     3661 cgccgccctg ttcttcctgg cggcgccggt tgcgggcctc ggcttgcacg atatcggcat
     3721 ccatgtcgtc cttgcgctcg ccgcgcttgg gcttggcttc atcctcttct cgcttggtgt
     3781 ggttggcggc ggcgacgcca agctgtttgc cgcgagcttg ctctggatcg gtcctgaagc
     3841 gatcgtccct ttcgccgtct acgccgcctt gatcggtggg gcgcttgcgc tccttctcct
     3901 gttctggcgc agcctgccgc tgccggcgac gcttgcctcg caaggctggc tcgtgcggtt
     3961 gcacagtcct gaacagggcg tgccctatgg catcgcgctc gcggcagcag ggctcctggc
     4021 atatccggcg acccgcttca tggccgcgct cggcggctga gtcggcattc gctccaagac
     4081 acaaggcgtg acccggccgg ttttggcacg gccattaacg cggcgttgag gattggcccg
     4141 cacagtaacg ggagttggac ggcgtgccgt cacgaggtca gtcattggag cgagccagtg
     4201 atcggcgacg ccgcattttc gctgggatcc gcgccgccga gcgagcccgt tccggatcgc
     4261 gaaagcgagg tgttcaaggc ctcgcgcctg cgctccctcc cgcgtgtcaa tatccaggct
     4321 ttttgcgagg atcaggacac ggccgcgtcg atcgagaagg cggcgcaaga ccggcgcctg
     4381 gcgagagcac gcgtgaacgt gcagatggga ggccttgacg cggcttgcgc cttctatcgc
     4441 aacgccgcca cgccaaatct catcatcgtc gaaaccctgc ttgagcgcgc gcggatgctc
     4501 gccgaactcg accgtctggc cgagctctgc gatcccgcca ccaaggtcct gatcgtcggc
     4561 cacatcaacg atgtgtttct caatcgcgag ctcgtgcgcc gcggcgtaag cgaatatctg
     4621 gtggcgccgg taggcgtgct ccagatcatc gaaagcctat caagcatcta cagcgagccg
     4681 gacgcgaagc ccgccggcca gacgatcgcc tttgtcgggg caaagggcgg cgcgggctcg
     4741 agcaccgttt gccacaacac cgcctttgcc atcgcgtccg cgctcgagcg cgaggtcgtg
     4801 atcgccgatt tcgatctccc tttcggcacg gccgggctcg atttcaacca ggatccgcta
     4861 aagggcatcg ccgacgcgct cgcctcgccc ggccgtctcg acgcgcacac gctggaccgc
     4921 ctgctgtcgc gctgctcgga ccaactgagc ttgttcgccg caccggccac gctcgatttc
     4981 gcttacgatc tcgatcctcg atcctgcgaa accgtgctcg acgtgttgcg cgaaagcgcg
     5041 ccctggatcg ccgttgatgt gccgcatctg tggacggcct gggcacgaga cgtggtgatg
     5101 cgcgccgacc acgtcgtcat caccgcagcc cccgatatcg ccaatctgcg caatgccaag
     5161 aacctgatcg accagatcaa gaccgcgcgc ccaaacgacc gcctgcctac gctcgtgctc
     5221 aatcaggtcg gtctgcacaa gcgtcccgag gtcgcaatca aggatttcgg cgatgccgtc
     5281 gcgctcgacc ccacggtcat catcgagttc gatgcgcaac tgttcggcac cgcggccaat
     5341 aacggtcaaa tgatcgagga ggtgtcgcac aagtcgcggg cgggcgaggc gttccgcaag
     5401 cttgccggca ttctgacgga ccggtccgag cagagcgcgg agcgcccatc gattctgtgg
     5461 tcgatgttga cccggctcgg acttgcaaga acgggcaggt agcgccgaac gtcctcgcgc
     5521 ttcaggccgt gcggctcctc gtctccgcct ccgcgccttc ggtccattct gccatcgccg
     5581 gaagcgcgag gagggcggcc gcatagcgcg cggccgtgcc gtcatcgccg tagcgcgcga
     5641 gatcgacgcc gtaggtgcgg aaccgggttg cgaccggcgc gtacatggcg tcggcattgg
     5701 aaaaagcacc gaacaggaac ggtccgcccg cgccgaaacg cgcccgcgtg tccttccaga
     5761 tcgcaacgac ccgcctgatc tcggattcga gcacggcatc gatgggcggg gagggcattc
     5821 ggccgagaag gtccatgggc atttcatggc ggagcgtggc gaaacccgca tgcatctcgg
     5881 cggagatgct gcgcgcggcc gcgcgcggct cctctttgcg cggccaaaag gcatattcgg
     5941 gatggcgttc ggcaagaaat tcgagaatgg cgagactgtc ccacacgacc ccccatttgg
     6001 tattgagcgc gggcaccttt cccgagggcg tatggcgcaa gatttcggcc ttggagtcgg
     6061 gctgcctcag cctgatgcgc gcctccgcga agtgaatgcc gcattgcttc aacgcaagcc
     6121 aggggcgcaa actccaggac gacagattct tgtcgccgat cacgagcgtg tagccattcg
     6181 ccgtcatgct gaagccttcc gccattgtca tgccgaagct cccctgccat taatgcggcc
     6241 ctccatcaag tccaggtttc agacattcag caatgaccag caaagcgacg acgctcgagg
     6301 aaacgcgccg cggattggaa gacctgctcc ttccccctgg ttccgaggcc ataccggttc
     6361 atttcatcgc ctcaagcgag gatccggcac tcgaaggttt gagcgcggcc gagcggagct
     6421 gggtcgaggc gcaagactgg aacgccaagc aaggctcggt cctgatcctg ccggacgcgc
     6481 atggcggcat cggcggggtc ttgctcggga ccggaggcgg tgacggtgcg gcgcaatcgc
     6541 cgcttttgac cggcgtcctc gccacgtcgg tcccggaagg cgactatcgc ctggcctcgc
     6601 cgcctgccgg tgccgaactc gccgtcctgg gcttcctcgc cgggagctac gccttcactc
     6661 gttacaaatc ggcgagcgcg gacaagaaga agcgcctggt cctgcccgag ggcgtggaca
     6721 gggacggtgt gctcgcgctc gccgaggcgc tttatttcgg ccgcgatctc atcaacacgc
     6781 cggcgaacga catggggccc gccgagcttg agagcgccgc gcgcgagctt gcaaccgcct
     6841 tcggcgccgg catcaaggtc accgaaggct cagggcttct ctcggacaat ttcccgatga
     6901 tccacgccgt cggacgggca agcgatcgca gtccccggct catcgatctc cgctggggcg
     6961 cggagcgcgc tcccaaggtg acgatcgtcg gcaagggaat ctgcttcgac acggggggac
     7021 tggacatcaa gccatcgagc gccatggcac tgatgaagaa ggacatgggg ggcgcggcct
     7081 cggcgcttgc gcttgcgctc ctcgtcatgc gcgcgaagct gccggtcagg ctccgggtgc
     7141 tgatcccggc ggcagagaac agcatctccg gcaacgcctt caggcctggc gacgtgctgc
     7201 cgagccgaaa tggcatgacg gtcgagatcg gcaataccga tgccgagggg cgccttgtgc
     7261 tcgccgacgc catcgcgctc gccgacgaag aggcgccgga ctatctcgtc accgtcgcca
     7321 cgctcacggg cgcggcgcgg attgcgctcg gacccgacct gccgcccctc tataccgacg
     7381 acgaggagtt tgcggcgcgg ctgctcgcat cggggctggc cgtcggggat ccgctctggc
     7441 ggatgccttt ctgggcgcct tacgacaagc agctcaagag cgcggtcgcc gatgtgagcc
     7501 atatcgccga gggaccgttc gccggctcca tcattgccgc tctgttcctc aggcgcttcg
     7561 tccggaatgc caaacgcttc gcccatctcg acattttcgg ctgggtgccg agcgagcagc
     7621 ccggcaggcc gaagggcggc gaaccgcaag gcgcccgcgc gctgttccac ttcttccgcg
     7681 aagagcttga aggatcgtga tcgataccgt cctccccgac ccgcgccggc atgcctatcg
     7741 cggcgacctg gcgtcggaag cgttgcgcgg gcggatcgag gcccggcgct tcgtcaaggg
     7801 cgagccgcgt caagtcgtgg cgccgtccct gccgttgcgc cgcgagccgc gcttcgatgc
     7861 cacgctcgac accgaggctc tgcgcggcga gaccctgacc gtctacgacg agagcgaggg
     7921 ttgggcctgg gtgcagcttt cccgcgacgg ttatgtcggc tacatgccga gcgaaggcct
     7981 gacgcggcgc atgatcgcgc cgacgcaccg gatcagcgcg ctcaggacct atgtctatcc
     8041 cgagcccgat gccaagacgc cgcctcttgc gctgctcagc atgaacgcgg gcatcaccgc
     8101 tcaacgcgat gagggaaaat tcctcgctct aggcggcggc gggtatgtgg ccgccattca
     8161 tgccgctccg atcggcgcac cttcgcccga tttcgtcgcc gtggcagagg cttttctcgg
     8221 cacgccctat ctttggggcg gccgcacgag catcgggctt gactgctcgg ggctcgtgca
     8281 gctcgcgctc gaggcggcgg gacacgccgc cccgcgcgat gccgacatgc aggcgcagga
     8341 gcttggcgcg gccatcgact ggcgtccggg cgctgctctt cgccggggag acttcgtttt
     8401 ctgggagggt catgtcggga tcatgacgag cccggcggat ttccttcacg ccaatgcatt
     8461 tcacatggcg gtcgcggccg agcccttcgc gcatgcgcgg gatcgcatca aggcggccgg
     8521 ttacgagatg acaggcgtgc gccggctggc agaggtcgcg gccgccggcg gcgccagacg
     8581 aggaccatga atcgaagatc ggtgcctccg tcgatgatcg agatccgatc atcgacgctg
     8641 cgacaccgca tggtcctcgt ctttcagtac gggggtgtcc gcaccatcaa cgcccttcga
     8701 gggcgttgag ttccaacgtc aagtcgccac agcaccgctc cgcgttgcct cgagctcgaa
     8761 ggacgccgcg agcagcgcct tggtgtaatc ggtcttgggg ctcgtgaaca cggtgcgcgc
     8821 ggggccttgc tccaccacct tgccgtgacg aagcacgatg atggagttcg caagcgcccg
     8881 cacgaccttg agatcgtggc tgatgaaaag ataggcaagc ttgtggcggc gctggagctc
     8941 gcgcaggaga tcgacgatct gcgcctgcac cgagacatcg agcgcggagg tcggctcgtc
     9001 gaggatgagg aatttcggtt cgagcacgag cgcgcgcgct atcgcaatcc tctggcgctg
     9061 gccgcctgag aattcgtgcg gatagcggtc gcggaaggcg ggatcgagcc cgacctcctt
     9121 cagcgcgcgc gccacgcgct ggtcgcgctc ggcgcgggtg agccccggat tctggatcgc
     9181 caggccctcc tcgatgatct gcgcgaccga gaggcgcggc gacagcgagc cgtaagggtc
     9241 ttggaacacg atctgcatat ggcgcctgag cggccgcatg cgtttcgaat cgtagccgtc
     9301 gatgcggtcg ccgagataga cgatcggccc ttcgctcgac acgagcctca ggacggcaag
     9361 accgagcgtc gtcttgcccg accccgattc gccgacaacg cccagtgtct ggccctcttt
     9421 gacttcgaga tcgatgccgt cgaccgcttt gatgtggccg accgtctgcc tgagaaagcc
     9481 gcgccggatc ggaaaccaca cgcgcaagtc cttggtcttc aggatcgtct ttgccgtgcc
     9541 gtcggccgcg ggcggcgaac ctttgggctc cgacgcgatg agatgcttgg tgtagggatg
     9601 cttgggggcg gcgaagatat cgcgcgtcgt gccccgctcg acgatttccc cgctgttcat
     9661 gacgcagacc ctgtcggcca tcttccgtac gatgccgaga tcgtgggtga tgagcagcat
     9721 cgccatgtgg aactcggcct tgagcttgag cagcaggtcg aggatttgcg cctggatcgt
     9781 cacgtcgagc gccgtcgtcg gctcgtcggc gatcaggagg tcgggctcgt tggcgagcgc
     9841 catggcgatc atcacccgct gccgctgccc gcccgagagc tgatgcgggt aggactggag
     9901 ccggcccttc ggatcgtcga tgccgacctt gtcgaggaga tcgagcacgc gcacgcgtgc
     9961 ggtcttgtcg gacatcctgc ggtgaatctt caacacctcg cccacctgct gctcgatcgt
    10021 gtgcagcggg ttgagagagg tcatcggctc ctggaagatc atcgagatct tgttgcccct
    10081 gacggcgcgg agcgcactcg aagggagcgt caggaggtcc tcgccgttga agcggatgcg
    10141 gcccgaaggg tgagacgcgg cggggtaggg gaggagccgc aagatcgaca acgccgtcac
    10201 cgtcttgcct gagcccgatt cgcccacgag cgccacggtc tcggccttgc cgatatcgaa
    10261 ggacacgcgg cggacggcgt gggtgacatc gtctccggca cggaaatcga ccgacagatc
    10321 ctcgaccgaa atcagggggc cgtttccgct catgccaatt ccttgaggat atccgccacc
    10381 gagcccgagt cttgtctcac cgaccgcgca ggtcgcgaat ctcttcgtcg atctccgtgg
    10441 ccgtcaggta cggcggccgc cgcgccgcga taatctcttc gagcgtcatg acgtgctcgg
    10501 gtggtttttc ctcttcgacc acggtcacgg tgacatgtcc ggcagggtcg attctctccc
    10561 ggagctccac cggaagctta gtggctgggt agtgctcttt cacgatcttg ttcatgaaaa
    10621 cactctatca cgccttggct ttggcgggat cggcgtcggc gcaagtcccg cgctcggttt
    10681 cgacggtagc gtggtcgagg ccgaagcgct ccttgagccg cgccttgatg cttctcacca
    10741 tgcgatcgga atcctcggtc tcgcagatca gggcatgcag cgtgaccatg cgccggctct
    10801 gggtgatcga ccagacatgc acgtggtgca ccgcttccac gcctttgacg ttggcgatga
    10861 ggtcgggtcc gatcgcccgc gtgtcgagct cctccggcgc cccttccagc agaatatgcc
    10921 cggcatccgc caccacgcgc cagccgctcc gcacgatgat ggcggcgaca aggatggaga
    10981 gcaggggatc gatgggtgtc cagccggtga tgaggatgac ggcaccggcg atcagcgccg
    11041 ccacggagcc gagcaggtcg ccgagaacgt ggatggccgc gctgcggata ttgaggttct
    11101 cgcggtcggc cccttgcagg agcgcgaagg cggcgatgtt cacgagaagc ccgagcccgg
    11161 cgaccaccac catgatgccg cccgaaacct cgggcgtcgt cgtcagccgc tcgatcgcct
    11221 catagacgac ccacgccgcg atcgcgaaca gcgcaagacc gttggcgaac gcgaccagca
    11281 cctggaaccg gtcgaagccg taggttcgct tccagtcggc gggacgccgc gccagacgaa
    11341 atccgaacca ggcgagcgca agcgaagcga agtcggtgag catgtggccc gcgtcggcaa
    11401 ggagcgcgag cgagcccgcg acgacgccgc ccacgatttc ggcgaacatg aaagcgccag
    11461 tcagacccgc tgccaatccc atccgccgct cgttgttgac gctcgctccg ccatgcgggg
    11521 ggcttacgtg atcgtggcga tgggagtgcg atccgtgcat gtgtctcacg tcaaggtctt
    11581 acgcgggtcg agcgcgtcgc gcaccgcctc gccgacgaaa atgagaaggc tcagcatgat
    11641 ggcgatcacg aagaaggccg tgagcccgag ccagggcgcc tgtaggttgt ccttgccttg
    11701 cgccagcagc tcgccgagcg atgccgaacc cggcggaagc ccgaaaccga gaaaatcgag
    11761 cgaggtcagc gtcgtgatcg agccgttcaa gatgaagggc atgaaggtga gcgtcgccac
    11821 catggcgttc ggcaggagat gcttgaaaat gatgcggaga tttgacaggc cgagcgcccg
    11881 cgccgccgtc acatattcga agttgcgtgc gcgcaggaac tccgcccgca cgacccccac
    11941 cagcgccacc caggaaaagg cgagcaggat gccgagcaga atccagaaat tcggctcgat
    12001 caccgcggcg acgatgatga ggagataaag ctgcggcacg ctcgtccaga tctcgatgaa
    12061 gcgctggaac aggaggtcgg tccagccgcc gaaatagccc tgaaccgcgc cggcggcgac
    12121 cccgatcagc gaggagaaga cggtgagcac gaggccgaac aggaccgaga tgcggaagcc
    12181 gtagatggcg cgcgcgacga cgtcgcggcc ctggtcgtcg gttccgagcc agttccattc
    12241 gagaacggcg ttgcaggggt ggaagccgcg ttccaccgcg agcttgcagt ccgcatcggt
    12301 aagaagccaa gtcggcgtca ctggaaacgc catcggcggg ttcttgttct gcgtgttgta
    12361 ggagaagcgg atcggaggcc acagcgtcca gcccccgccg tcggcgatca tcgccttgac
    12421 ggcagggtcg cgataatcgg cctcggtctc gaaaatgccg ccgaaggtcg tttccggata
    12481 ggccctgaag atcggcatgt agaggccgcc cttgtacgag acgaggatcg gcttgtcgtt
    12541 ggcgatgaac tcggcaaaca ggctgatcac gaacagcgcg agaaaaatcc acaacgacca
    12601 gaagccgcgg cggttcgact tgaagacctc ccagcggcgc cgctggagca cggaaagcca
    12661 aggccgcgcc ctcggcaacc cttgcgccgt cacctcctcg acgattgcgg tgtcggtcgg
    12721 agcgtccata tctcacacct cgcgcgtctc gaaatcgatg cgcggatcga cccaggtata
    12781 ggtgaggtcg gagaggagat tgatgagaag gccgagcagc gagaagatgt agaccgtggc
    12841 gaacacgacc ggatagtcgc ggttcacgat cgattcgaag ccgagcagtc ccagcccgtc
    12901 gagcgagaag atcgtctcga tcagaagcga gccggcgaag aacgcgccga tgaacgcacc
    12961 ggggaagccc gcgatgacga tcagcatggc gttgcggaac acgtggccgt agagcacgtg
    13021 gcgctcggga aggcccttgg cccgcgccgt catcacatat tgctttcgga tctcctcgag
    13081 gaacgagttc ttggtgagga acgtcgtggt ggcgaaggcc gacagcgcca tcgcgatcag
    13141 cggcagaaca agatgccaga aataatcggc gatcttcagg taccagggca gctcccacca
    13201 attgtcg
//
LOCUS       DASUQP010000120        15720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1557271, whole genome shotgun sequence.
ACCESSION   DASUQP010000120 DASUQP010000000
VERSION     DASUQP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15720
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557271"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFY74_05355"
     CDS             complement(<1..600)
                     /locus_tag="VFY74_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5931165.1"
                     /translation="MTAERTPGFNTLAIHAGAQPDPATGARATPIYQTTSFVFDNVEH
                     AAALFNLETFGNIYTRIMNPTQAVLEERVAALEGGTAGLAVASGHAAQALVFHTLLEP
                     GDEFIAARQLYGGSINQFTHSFKKCGWEVRWADTTDPASFRAALTPKTKAIFVESIAN
                     PGGIITDIEAVARVAKEAGVPLIVDNTLASPYLIRPVDYG"
     gene            complement(645..1166)
                     /locus_tag="VFY74_05360"
     CDS             complement(645..1166)
                     /locus_tag="VFY74_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX5931166.1"
                     /translation="MNHDAYSDAYIRDILTKYRTIAMVGASPTTSRPSYFAMKYLKEK
                     GFRVIPVNAGQEGKTILGEKVYGSLAAIPEKVDIVDIFRSSKSALGIAREAIAIGASV
                     VWMQLGVRNDEAARLAEDAGLQVVMNRCPKIEYGRLSGELNWAGVNSRMLSSKRPLLG
                     AKGVQHRVIATKA"
     gene            complement(1204..2022)
                     /locus_tag="VFY74_05365"
     CDS             complement(1204..2022)
                     /locus_tag="VFY74_05365"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5931167.1"
                     /translation="MASIATQPLRDAQILLREDQGPIAVLTLNRPEARNSLSRELIDA
                     VTGAMREIGDADAIRAVVVTGAGSAFSSGHDLREMTAHRNDRDRGRAFFAETMSASAE
                     MMLAIVRSAKPVIAAVNGIATAAGCQLVASSDLAVASTEARFATPGVNIGLFCSTPMV
                     ALSRNVSRKAAMEMLLLGEMLGAEEAKSIGLVNRVVEPDHVLNEAIEFGRKIATKPKL
                     TLKTGKEAFYRQLDMTLEEAYRYASAVMVENMLDAEAEEGIGAFLDKRTPRWPR"
     gene            2234..2698
                     /gene="rplM"
                     /locus_tag="VFY74_05370"
     CDS             2234..2698
                     /gene="rplM"
                     /locus_tag="VFY74_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5931168.1"
                     /translation="MKTFSATPKDIEKTWTLIDADGLVVGRLAVLIANRLRGKHKPGY
                     TPHLDCGDNIVVINAGNIVLTGNKRADKVYHWYTGYPGGIKDRTAAKILEGKHPERVL
                     QKAVERMLPRGPLGRQQLRNLRIYPGPDHPHAAQSPVTLDVAALNAKNARSA"
     gene            2703..3182
                     /gene="rpsI"
                     /locus_tag="VFY74_05375"
     CDS             2703..3182
                     /gene="rpsI"
                     /locus_tag="VFY74_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5931169.1"
                     /translation="MSETMTLEDLAPLKPATNAPQARVYEKKVDAQGRAYATGKRKDA
                     VARVWVKRGPGKITINGKDHAAYFARPVLQMILKQPLVTSKREGQYDIVCTVSGGGLS
                     GQAGAVRHGISKALTRYEPELRSVLKKGGFLTRDARTVERKKYGKAKARRSFQFSKR"
     gene            3294..4343
                     /gene="argC"
                     /locus_tag="VFY74_05380"
     CDS             3294..4343
                     /gene="argC"
                     /locus_tag="VFY74_05380"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX5931170.1"
                     /translation="MAKTKTIRAAVLGASGYTGADMIRLAARHPAIEIGALIAKGHAG
                     MSLAQVFPHLSSLDLPALVSPDQADWDEIEVAFCGLPHGTAHGEIGKLPKRIKVIDMS
                     ADFRLHDASAYAHWYGNQHSAPGMLKDAVYGLTEHYREKIRGARLVACPGCYPTGVLL
                     ALLPLARTKLIETTDIIIDAKSGVSGAGRSLKQNILFSEAGEGLSPYGIGNHRHVPEI
                     EQELGIASGSPVTVNFTPHLAPMSRGELCTSYVRIAGKIRAADLRKALTAAYADSPFV
                     HVVAEDTIPATQHVRGSNFCHVGVFDDRIQGRAIIVSAIDNLVKGSAGQALQNFNVMY
                     GLAETTGLEQLPLFP"
     gene            complement(4349..6166)
                     /gene="phaC"
                     /locus_tag="VFY74_05385"
     CDS             complement(4349..6166)
                     /gene="phaC"
                     /locus_tag="VFY74_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX5931171.1"
                     /translation="MQEKPKDPGASQGEETEPSQFEALARNMVRLFDQGAKAASTLAE
                     RSQPNGQGPYSMAAEVGEAAKSLGEIARHWATDPAKLAAAQSVLFKSYADLWGRSVRR
                     FLGEDVEPVVEPEPGDNRFKDPDWSNTQIFDFWKQSYLITSRWAEDLTRNTEGIDEKT
                     RKKALFYLNQMLAALSPSNFPLTNPEVVRTTLQTNAENLVQGMSHFVHDLEHSKDLLR
                     ISQTDLSAFEIGRNLAVTPGKVVFQNDLIQLIQYAPATEEVYERPLLIVPPWINKYYI
                     LDLVPQKSFVKWAVEQGFTVFLVSWVNPDARLSQKTFEDYMHEGILAAIDAVVRQTGS
                     PKINALGYCVGGTLLAATLAYMAAKKDNRVVSASFLAAQVDFSEAGDLLVFIDDTQLK
                     ALEEMMAEQGYLDGSRMAAVFNMLRPRDLIWPYVVNNYLLGKKPFPFDLLYWNADSTR
                     LPAANHAFYLREFYHLNRLAKGEMQLGGVKLDLSRVKIPIYELFTKEDHIAPAASVFR
                     GSKLFGGPVRHAMAGSGHIAGVVNPPANNKYQYWSGGRAGSLEEWIATARETPGSWWP
                     DWTSWLASYSGPKVPARNPSNGPLWAIEDAPGSYVRNRG"
     gene            6321..6584
                     /locus_tag="VFY74_05390"
     CDS             6321..6584
                     /locus_tag="VFY74_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931172.1"
                     /translation="MLRAHFSALRLSALLLVFGLSACADTMAVEDSPPTSTQLQRHYD
                     KTLTKAEQQAVISDIQSATAKKRDEADKEDTGATGTEAGEKTN"
     gene            6684..7916
                     /locus_tag="VFY74_05395"
     CDS             6684..7916
                     /locus_tag="VFY74_05395"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX5931173.1"
                     /translation="MTSDFHRIKRLPPYVFEQVNKLKAEARARGDDIIDFGMGNPDMP
                     TPEHIVAKLNETLRDPRTSRYSASKGIGGLRRAQAAYYRRRFGVKLNPETQVIVTLGS
                     KEGFANTAQAITAPGDVILVPNPTYPIHEFGFIMSGGVIRHLPSRTDSEFLASLDRAI
                     RHSVPKPSVLVLNYPANPTAEVADLDFYREVVAFAKKHDLIVLSDLAYAEIYFEGEPP
                     PSILQVPGAIDIALEFTTLSKTYAMPGWRVGFAVGNERLIHALTRVKSYLDYGAFTPI
                     QVAAAAALNGPQDCVAEIRATYKRRRDCLVDAMKRTGWPVPSPPATMFAWVPLPEMFA
                     HLGSLEFSKLLLEKASVAVSPGVGFGEYGEGYVRIALVENEQRIRQAARNIRRFLADS
                     AKTLHNVIPIASKANRSF"
     gene            7926..9251
                     /locus_tag="VFY74_05400"
     CDS             7926..9251
                     /locus_tag="VFY74_05400"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5931174.1"
                     /translation="MMDGLKLGVAGVGTVGSGLLRLLAARKGDLAARAGRPIEVVAVS
                     ARNRQKNRDADLSGLRFVQDPVALAADPAIDVFVELIGGEDGAAKNAVEAALASGKHV
                     VTANKALLAHHGPSLAALAEKNGVALNFEAAVAGGIPVVKVIRESLQGNDISRVYGIL
                     NGTCNYILTLMEKEGRSFADVLKEAQAQGYAEADPSFDIGGFDTAHKLSLLTSLAFGT
                     RTSFESVYVEGIEQITPADIEAAGDLGYRIKLLGVALRTESGIEQRVHPTMVPKHRAI
                     AEVDGVSNCVAIDGDFVGDVMLVGPGAGAGPTASAVTSDLIDIARGHILPPFGTKAKA
                     LGPYKKAQMQAHEGGYYIRLSVYDRPGAFAAIAGRMADQGISIESIVQRRPGAELPGF
                     HRRDEGASPAFVVLITHQTTEAAIRKALAAIERDGHVDQKPQMIRIEKL"
     gene            9313..10302
                     /gene="glpX"
                     /locus_tag="VFY74_05405"
     CDS             9313..10302
                     /gene="glpX"
                     /locus_tag="VFY74_05405"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5931175.1"
                     /translation="MSKAPAKTELDRALALDIVRVTEATAVAAALWRGRGNEAAADEA
                     AAEAMYRELERLAIEGVVVVGEGEENETPLLYIGETLSSGGTPGLKVDLAVDPVEGST
                     LCAKALPNALSVLAVAEHGSLLKVPGIYMDKIAIGPGYPEGLVDLDATPADNVNALVL
                     AKNVPISDITVCILDRPRHAKLIEEVRATGAALRLIGDGDIAGVIHVTKPMETGTDIY
                     MGIGGAPEGVLAAAALACIGGQMQGRLVASNEQQRSRARAAGILDLNRKFHIHDMVTG
                     DLAFAATGITDGSLLEGVRFTKDAIFTHTLVMRASTRTVRWIKAEHPDISKFD"
     gene            10380..12209
                     /gene="recJ"
                     /locus_tag="VFY74_05410"
     CDS             10380..12209
                     /gene="recJ"
                     /locus_tag="VFY74_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5931176.1"
                     /translation="MTAALKFQANEIASAFLGVERSARGYRWVERLDPARALTAAAIS
                     QAHSLPELIGRVLAARGADIQTATRYLDPSLRTLLPDPARLEDMEKAAARFASAIAQN
                     EPIAVFGDYDVDGAASVALVERFLRAHGQSVATYIPDRLTEGYGPSPESLAGLAKDGA
                     RLILTVDCGTTAEIAIAAANAAGAEVIVIDHHQADEALPPAFAVLNPNRQDDLSGQGH
                     LAAAGVVFLFLVATTRALRREGFYGNRPEPDLLGLLDLVALASVCDVVPLRDVNRAFV
                     AQGLKVLRLRKNQGLRALADAARIDAAPTCYALGFVLGPRINAGGRVGASSLGARLLA
                     TDDDIEARALAAKLEAFNAERKAIEERMLEEAFASAETSLAADPGSPLLFLAAAGWHK
                     GLLGLVAGRVAERFRLPAFVSSLEPDGFATGSARSIASVDLGAAVRSALHEGLLAKGG
                     GHAMAAGFRFERRKEERLVAFLRARLAAPVAQAGTVRALAIDGALSAGGANKDLMDQL
                     DRAGPYGPGHPEPRFVFPAHRLARVRSIKDAHLRCTLKAPDGARLEACAFRIADTPLA
                     KLLLDGEGQTVHVAGHLRRTSWQGRESVELLIEDAAATHRSNQ"
     gene            12524..12598
                     /locus_tag="VFY74_05415"
     tRNA            12524..12598
                     /locus_tag="VFY74_05415"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12557..12559,aa:Glu,seq:ttc)
     gene            complement(12606..13025)
                     /locus_tag="VFY74_05420"
     CDS             complement(12606..13025)
                     /locus_tag="VFY74_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5931177.1"
                     /translation="MKYLHTMVRISDVDQSLDFYCNKLGLKEVRRMENPQGRYTLFYL
                     AAPEDAEAEIELTYNWDPEAYGEGRNFGHLAFAVDDIYATCDRLMKKGVVINRPPRDG
                     HMAFIRSPDNISIELLQRGKPKPKQEPWASMPNIGKW"
     gene            13494..14066
                     /locus_tag="VFY74_05425"
     CDS             13494..14066
                     /locus_tag="VFY74_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5931178.1"
                     /translation="MGAKEPPLEEGHTQRDEEATVDVFEVSGSIKWFDASKGYGFIVP
                     DEDLPDVLLHVTCLRRDGFQTAYEGARVVCEVLRRPKGLQAFRIRAMDDSTAIHPSQL
                     PQRTHVIVVPESDWEQATVKWFNRLRGFGFLTRGAQSLDIFVHMETLRRCGFTELRPG
                     QSLLVKYGRGPKGLMAAELKPDSAQGPSSH"
     gene            14099..14539
                     /locus_tag="VFY74_05430"
     CDS             14099..14539
                     /locus_tag="VFY74_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX5931179.1"
                     /translation="MAGFLFGGALLAQAVAFAASAGTIVLKTETGDHSFDIEVMTTDR
                     ERAHGLMYRRSLPERSGMLFLYDRPQGATMWMKNTFIPLDMVFIAPGGTVHRIERNAE
                     PFSTEVISSEGAIIAVLELNAGEADRIALKRGDKVIYPGLGESP"
     gene            14922..14998
                     /locus_tag="VFY74_05435"
     tRNA            14922..14998
                     /locus_tag="VFY74_05435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14956..14958,aa:Pro,seq:tgg)
     gene            15033..15335
                     /locus_tag="VFY74_05440"
     CDS             15033..15335
                     /locus_tag="VFY74_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEX5931180.1"
                     /translation="MAARIYKPAKTAMQQGMAPRDWVLEYEPDEAQVIEPLMGWTSSF
                     DTKSQVRLRFTTKEEAVAFAVRHGIAFRLEEPKETELRPKSYAENFKFGRPDRWTH"
     gene            15387..15462
                     /locus_tag="VFY74_05445"
     tRNA            15387..15462
                     /locus_tag="VFY74_05445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15420..15422,aa:Arg,seq:tct)
     gene            15484..>15720
                     /locus_tag="VFY74_05450"
     CDS             15484..>15720
                     /locus_tag="VFY74_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931181.1"
                     /translation="MLDGSEKWIGPFQVRKMLEHCLDDSVVPKALESGSAYLVTRKDW
                     RTAPTHESIPLYVGGNTGEAPRFRTRVGDLLADAF"
BASE COUNT         2803 a         5060 c         5017 g         2840 t
ORIGIN      
        1 accgtagtcc acaggccgga tgagataggg cgaagcaagc gtgttgtcga cgatgagcgg
       61 cacgcctgcc tccttggcga cgcgcgcgac ggcctcgatg tcggtgatga tgccgccggg
      121 attggcgatc gactcgacga agatcgcttt ggtcttggga gtaagcgcgg cgcggaacga
      181 ggccggatcg gtcgtatcgg cccagcgcac ctcccagccg cacttcttga acgagtgggt
      241 gaattggttg atcgacccgc catagagctg gcgcgcggcg atgaactcgt cgcccggctc
      301 gagcaaagta tggaatacga gcgcctgcgc cgcgtgaccc gaagccacgg caaggccggc
      361 ggtgccgcct tcgagtgccg ctacccgctc ctccagcacc gcctgagtcg ggttcatgat
      421 gcgggtgtag atgttgccga aggtctccag attgaacagc gccgccgcat gctcgacatt
      481 gtcgaacacg aaggaggtgg tctgatagat cggcgtggca cgggcgccgg tggcgggatc
      541 gggctgggcg ccggcgtgga tcgcaagcgt gttgaagccg ggcgttcgct ccgcggtcat
      601 cgtcatggtc ccttagggct aatggttggc gcgactatac cggctcaagc cttcgtggca
      661 atcacgcggt gctgcacgcc ttttgccccg agcaggggcc gcttgctcga cagcatgcgg
      721 gagttcaccc cggcccaatt cagttcgccg gacagccgtc catactcgat cttcgggcag
      781 cggttcatca ccacttgaag gcccgcgtcc tcggcgaggc gcgccgcctc gtcgttgcgg
      841 acgccgagtt gcatccacac cacggaagcg ccgattgcaa tcgcctcgcg agcgatgcca
      901 agcgcggact tcgagctgcg aaaaatgtcg acgatgtcga ccttctccgg gatcgcggcg
      961 agcgagccat acaccttctc gccaaggatt gtcttgcctt cttgccccgc attgaccggg
     1021 atgacgcgaa aacccttctc cttcaaatat ttcatggcga aatagctcgg gcgcgaggtg
     1081 gtcgggctcg cccccaccat ggcgatcgtg cggtactttg tcagaatgtc cctgatatag
     1141 gcgtcgctat aggcgtcgtg gttcatcgac tggtcgtctt tccgggcgaa gtgcggcgcg
     1201 ggcctagcgc ggccagcgcg gcgtgcgctt gtcgagaaac gcgccgatgc cttcctccgc
     1261 ctcggcgtcg agcatgttct cgaccatgac ggcggatgcg tagcggtagg cttcttcgag
     1321 cgtcatgtcg agctgacgat agaaggcctc cttgccggtc ttgagcgtca gcttgggctt
     1381 cgtcgcgatc ttgcggccga actcgatcgc ctcgttcaaa acgtggtcag gctcgaccac
     1441 acgattgacg aggccaatcg acttggcctc ctcggcgccc agcatctcgc cgaggagcag
     1501 catctccatc gccgccttgc gcgacacgtt gcggctgagc gcaaccatgg gcgtcgagca
     1561 gaagaggccg atattgacgc cgggcgtggc gaagcgcgct tccgtgctcg ccacggcaag
     1621 atctgagctc gcgacgagct ggcagccggc tgccgtggca atgccgttca cggccgcgat
     1681 caccggtttt gccgagcgga cgattgcgag catcatctcg gcggaggcgg acatcgtctc
     1741 ggcaaagaag gcgcggcccc tgtcgcggtc gttgcgatgc gccgtcatct ctctgaggtc
     1801 gtggccggaa gagaaggccg agcccgcgcc cgtcaccacg acggcgcgaa tcgcgtcggc
     1861 gtcgccgatc tcgcgcatgg cgccggtcac agcgtcaatc agctcgcgcg acagcgaatt
     1921 gcgcgcctcg ggccgattga gcgtgaggac ggcgatgggt ccttggtcct cacgcagcaa
     1981 gatctgcgcg tcgcgtaatg gctgtgttgc aatactggcc atgcgcctgc ctctcggaat
     2041 cggtgggagc cttatagcag agattctttt cgaccgacgc tttgccggcg ccgggcgcgg
     2101 gccgggggag ggggctcaaa tcagattatg ataccacatc actaaccggc tgaattcgat
     2161 taaggtctgt atcttcttga cggcggctga ggcggaagct agaagcgccg atcctgcaag
     2221 ccccggatca cccatgaaga ccttctccgc cacgccgaag gacattgaaa agacctggac
     2281 cctcatcgac gctgacggcc tcgtggtggg cagacttgcc gtgctgatcg cgaacaggct
     2341 cagagggaaa cataagccgg gctacacccc gcatctcgat tgcggcgaca acatcgtcgt
     2401 catcaatgcc ggcaatatcg tgctcaccgg caacaagcgc gccgacaagg tctatcactg
     2461 gtacacgggc tatcccggcg gcatcaagga ccggaccgcc gccaagatcc tcgaaggcaa
     2521 gcatcccgag cgggtgttgc agaaggccgt cgaacggatg ttgccgcgcg ggccgcttgg
     2581 ccgccaacag ctccggaatt tgcgtatcta tccggggccc gaccatcccc acgcggcgca
     2641 gagtccggtg acgctcgacg ttgccgccct caacgccaag aatgcaagga gcgcctgagg
     2701 ccatgagcga gaccatgaca cttgaagatc ttgctccgct gaagcccgcc acgaacgccc
     2761 cgcaggcccg cgtctacgag aagaaggtcg acgcgcaagg ccgcgcctat gccaccggca
     2821 agcgcaagga cgccgtcgcc cgtgtctggg tgaagcgggg tcccggcaag atcaccatca
     2881 acggcaagga ccatgccgcc tacttcgcgc ggcccgtgct ccagatgatc ctgaaacagc
     2941 ccttggtcac ctcaaagcgc gaaggccagt acgacatcgt ctgcaccgtc agcggcggcg
     3001 ggctctcggg ccaggccggc gccgtcagac acggcatctc caaggcgctc acccgctacg
     3061 agcccgaact ccgcagcgtc ttgaagaagg gcgggttcct gacccgcgac gcccgtaccg
     3121 tggagcgcaa gaaatacggc aaggccaaag cgcgacgaag cttccagttc tcgaagcgct
     3181 agccgcttcc tcccgcgcgg gatcgattgc cggacaccgg ctttcgccgg tatgtcgtgg
     3241 tcccgcgatt cgggctaaac cctctctcat cattcgtgtt tgaggcctcg accatggcca
     3301 agaccaaaac catcagagcc gccgtgctcg gtgcttccgg ctacaccggc gccgacatga
     3361 tcaggctcgc ggcacgccat cccgccatcg agatcggggc gctgatcgcc aagggccatg
     3421 cgggcatgag cctcgcccaa gtgttcccgc acctttcgag cctcgatctg ccggctcttg
     3481 tcagccccga tcaggccgat tgggacgaga tcgaggtcgc cttctgcggg ctgccgcacg
     3541 gcacggcgca tggcgagatc ggcaagcttc cgaagcggat caaggtcatc gacatgtcgg
     3601 ccgacttccg cctgcacgat gcgagcgcct atgcccactg gtatgggaac cagcacagcg
     3661 cgccggggat gctcaaagac gccgtctacg gcctcaccga gcattatcgg gaaaagatca
     3721 gaggcgcgcg gctcgtggcc tgtcccggct gctatccgac cggcgtgctg cttgcgctcc
     3781 tgccgcttgc aagaacgaag ctgatcgaaa ccaccgacat catcatcgac gcgaagtcgg
     3841 gcgtgtcggg ggccggccgg agcctgaagc agaacatcct cttctcggaa gcgggcgagg
     3901 ggctttcgcc ttacggcatc ggcaatcacc gtcacgtgcc tgagatcgag caggaattgg
     3961 gcatcgcttc cggttcaccc gtcaccgtca atttcacgcc gcaccttgcg ccgatgtcgc
     4021 gcggcgagtt gtgcacgtcc tatgtgcgga ttgccggcaa gatccgcgcc gccgatcttc
     4081 gcaaagcgct gaccgcggct tatgcggaca gccccttcgt gcatgtcgtg gcggaggaca
     4141 cgatccccgc gacccagcac gtgcgcggct ccaatttctg ccatgtcggc gtgttcgacg
     4201 accgcatcca agggcgcgcc atcatagtct cggcaatcga caatctggtg aagggttcgg
     4261 ccgggcaggc tctgcaaaat ttcaacgtga tgtacggcct cgccgagacg accggcctcg
     4321 agcagctccc gctcttcccc tgacgccgct agccgcggtt cctcacgtag gagcccggcg
     4381 cgtcctcgat cgcccagagc ggcccgttcg aggggttgcg ggcgggaacc ttgggccctg
     4441 aataggaggc aagccaggag gtccagtcgg gccaccaaga gcccggcgtt tcgcgtgccg
     4501 tcgctatcca ctcttcgagc gaccccgcac gtccgcccga ccagtactga tacttgttat
     4561 tggccggagg attgaccacg ccggcgatat ggcccgagcc cgccatcgca tggcgcaccg
     4621 gcccgccgaa caacttgctg ccccgaaaga ccgaggctgc cggcgcgatg tggtcttcct
     4681 tggtgaagag ctcgtagatc gggatcttca cgcgcgacag gtcgagcttg acaccgccga
     4741 gctgcatctc tcccttggcg agccggttca agtgatagaa ctcgcgcaag tagaaggcat
     4801 ggttggcggc gggtagccgg gtggagtcgg cgttccaata gagaagatcg aacgggaagg
     4861 gtttcttgcc gagcaggtaa ttgttgacga cgtaaggcca gatcaggtcg cgcggcctga
     4921 gcatgttgaa caccgccgcc atgcgcgatc cgtcgagata gccctgttcg gccatcattt
     4981 cctcgagcgc cttgagctgc gtgtcgtcga tgaacacgag caggtcgccg gcctcgctga
     5041 aatcgacctg agcggccagg aagctcgcgg agaccacgcg gttatctttc ttggctgcca
     5101 tgtaggcaag agtcgcggcc agcagggtac cgccgacaca gtagccaagc gcattgatct
     5161 tcgggctgcc ggtctggcgg accaccgcgt cgatcgcggc gaggatgcct tcgtgcatgt
     5221 agtcctcgaa agtcttttgc gataggcgcg cgtcgggatt gacccaagag acgaggaaca
     5281 cggtgaagcc ctgctccacc gcccatttta cgaaggactt ctgcggcacg agatcgagga
     5341 tgtagtactt gttgatccag ggcggcacga tgagaagggg ccgctcgtaa acctcctccg
     5401 tagcgggcgc gtattggatg agctggatga ggtcgttctg aaacacgacc ttgccgggcg
     5461 tcaccgcgag atttcgtccg atctcgaaag cggagaggtc cgtctggctg atccggagca
     5521 aatccttcga gtgttccagg tcgtgaacga agtgcgacat gccctgcacc aaattctcgg
     5581 cattggtttg cagcgtcgtg cgaaccactt ccggattggt gagcgggaag ttcgacggcg
     5641 atagcgccgc gagcatctgg ttcagataga agagcgcctt cttgcgcgtc ttctcgtcga
     5701 tgccctctgt gttgcgcgtg aggtcttcgg cccagcgcga ggtgatcagg taggactgtt
     5761 tccagaaatc gaagatttgc gtattggacc agtcgggatc cttgaagcga ttgtcgccgg
     5821 gttcgggttc gaccacgggc tcgacgtcct cgccgagaaa acggcggacg gaacggcccc
     5881 agagatcggc atagctcttg aagagcacgc tctgcgcggc ggcgagcttg gcggggtcgg
     5941 tcgcccaatg gcgcgcaatc tcgcccagcg acttagcggc ctcgccgacc tccgcggcca
     6001 tgctgtaggg cccctgccca ttcggctggc tgcgctcggc cagagtcgag gcggccttgg
     6061 ccccttgatc gaagaggcgc accatgttgc gggcaagcgc ttcgaactgg ctgggttcgg
     6121 tttcttcacc ctggcttgcg ccggggtcct tgggcttctc ctgcatcaaa aagtccttgc
     6181 tttaccccca cgtaacctcg ctggcgccaa tgcggtttcg tactccgcgg ctgtaccaga
     6241 caaggcatta ttgtgctatg caatatagac gacgattgca aatcgccaag ggaaaccacc
     6301 agaggcaacc gctgaattac atgctgcgcg cccacttttc agctttgcgc ctgtccgccc
     6361 tcctgctcgt tttcggactt tccgcctgcg ccgacacgat ggccgtcgag gattcgccgc
     6421 ccacttcgac ccaactccaa cggcactacg acaagaccct gaccaaggcc gagcagcaag
     6481 cggtgatcag cgatatccag agcgcgacgg ccaagaaacg ggacgaggcc gacaaggagg
     6541 acaccggcgc gaccggcacg gaagccggcg aaaagaccaa ctagagaccg gatttcgccg
     6601 gggcgatcgg cttgcccctc cgcccttttt tatggttcaa gtgagcgccc ttcagggtct
     6661 gaaccggggt agggaccctc gcagtgacca gcgatttcca cagaatcaag cgccttccgc
     6721 cctacgtctt cgagcaggtc aacaagctca aggccgaggc ccgcgcccgt ggcgacgaca
     6781 tcatcgattt cggcatgggc aatcccgaca tgccgacgcc cgagcatatt gtcgccaagc
     6841 tgaatgagac gctgcgcgac ccgcgcacga gccggtattc cgcgtcgaag ggcatcgggg
     6901 ggctccgtcg cgcccaagcc gcctattacc ggcgtcgctt cggcgtgaag ctcaatcccg
     6961 agacgcaggt catcgtcacg cttggctcca aggagggctt cgccaatacc gcccaggcga
     7021 tcacggcgcc gggcgacgtc atcctggtgc cgaacccgac ctatcccatt cacgaattcg
     7081 gcttcatcat gtcgggcggg gtcatcaggc atttgccgtc gcgcaccgat tccgagttct
     7141 tggcctcgct cgacagagcc atacgccact cggtgccgaa gccctcggtg ctcgtgctga
     7201 attatcccgc caatcccacc gccgaggtgg ccgacctcga cttctatcgg gaggtcgtgg
     7261 ccttcgccaa gaagcacgac ctgatcgtgc tctccgatct cgcttatgcc gagatctatt
     7321 tcgagggcga gccgccgccg tcgatccttc aggtgcccgg cgccatcgat atcgctctcg
     7381 agttcaccac gttgtcgaag acctacgcca tgcccggctg gcgcgttggc ttcgccgtcg
     7441 gcaacgagcg cctcattcat gctctcaccc gcgtaaaatc ctatctcgat tatggggcct
     7501 tcacgcccat ccaggtggcc gcggccgcag cgctcaacgg gccgcaggac tgcgtcgccg
     7561 agatccgcgc gacctataag cgccgccgcg attgcctcgt cgacgccatg aagcgcaccg
     7621 gctggccggt gccttcgccg cccgccacca tgttcgcctg ggtcccgctc cccgagatgt
     7681 tcgcccatct cggctcgctc gaattttcga aacttctgct ggaaaaggcc tcggtcgcgg
     7741 tgtctcccgg cgtcggattc ggcgagtatg gcgaaggcta tgttcgcatc gcgctcgtcg
     7801 agaacgagca gcgcatccgc caggccgcgc gcaatatccg gcggttcctc gccgactcgg
     7861 ccaagaccct gcacaacgtg attcccattg catccaaggc caatcggtcg ttctgatcct
     7921 ctcccatgat ggatggattg aagcttggtg tggccggcgt cggcaccgtc ggcagcggcc
     7981 tgttgcggct ccttgccgcg cgcaaggggg accttgccgc gcgggccggg cgccccatcg
     8041 aggtcgttgc cgtatcggcg cggaaccgtc agaaaaatcg cgacgccgat ctgtccggtc
     8101 tccgcttcgt gcaggatccg gtcgcgctcg ccgccgatcc cgcgatcgac gttttcgttg
     8161 agctgatcgg cggcgaagac ggcgcggcca aaaacgccgt cgaggcggcg ctcgcgtccg
     8221 gcaagcatgt cgtcaccgcc aacaaggcgc tgctcgccca tcacggaccg agccttgccg
     8281 cgctcgccga gaagaacggc gtggcgctga atttcgaggc ggcggtggcg ggcggcatcc
     8341 ccgtggtcaa ggtcatccgc gagagcctgc aaggcaacga catctcgcgc gtctacggca
     8401 tcctcaacgg cacctgcaat tacattttga cgctgatgga gaaggagggc aggagcttcg
     8461 ccgacgtgtt gaaggaggcg caagcccaag gctatgccga ggccgatccg agtttcgata
     8521 tcggcggctt cgataccgcg cacaagcttt cgcttctgac aagcctcgcg ttcggcacgc
     8581 gcacgagctt cgaatccgtc tatgtcgaag gcatcgagca gatcacgccc gccgacatcg
     8641 aggcggcggg cgacctcggc tatcgcatca agttgctggg cgtggcactc cgcaccgaga
     8701 gcgggatcga gcagcgcgtg cacccgacga tggtgccgaa gcatcgggcc atcgccgaag
     8761 tcgacggcgt atcgaactgc gtggcgatcg acggcgactt tgtcggcgac gtcatgctgg
     8821 tcggtccggg cgcgggcgca ggccccaccg cctcggccgt gacgagcgac ctgatcgaca
     8881 tcgcccgcgg ccacattctc ccgcccttcg gcaccaaggc caaggcgctc gggccctaca
     8941 agaaggcgca gatgcaggcc catgaaggcg gctattacat caggctctcg gtctacgacc
     9001 ggcccggcgc attcgcggca atcgccggcc gcatggcgga tcagggcatc tcgatcgaaa
     9061 gcatcgtgca gcgccgcccg ggcgctgagc ttcccggctt ccaccggcgg gacgagggtg
     9121 cgtcgccagc gtttgtcgtg ctgatcaccc atcagaccac cgaggccgcg atccgcaagg
     9181 ctttggccgc gatcgagcgc gacggtcatg tcgatcaaaa gccgcagatg atccgcatcg
     9241 aaaaactgtg atagtgagtg tgctaaccct ccaggcggcg ccgcgcgtgc ctgagggcgg
     9301 ggaggaacgt acgtgtcgaa ggctccggcg aagaccgagc tcgaccgcgc tttggcgctc
     9361 gatatcgtac gtgtgaccga ggccacggcg gtggctgccg cactgtggcg gggccgcggc
     9421 aacgaagcgg ccgcggatga ggccgccgcc gaagccatgt accgcgagct tgagcggctt
     9481 gcgatcgaag gggtcgtggt cgtcggcgag ggggaggaga acgagactcc gcttctctat
     9541 atcggcgaaa ccttgagcag cggcggcacg ccggggctca aggtggatct cgccgtcgat
     9601 cccgtcgaag gctcgacgct ctgcgccaag gcgctgccca atgcgctctc ggtgctcgcc
     9661 gtggccgagc atggcagcct gctcaaggtg ccggggatct atatggacaa gatcgccatc
     9721 ggccccggct atcccgaggg tctggtcgat ctcgacgcga cacccgccga caacgtcaac
     9781 gcgctggtgc tcgccaagaa cgtgccgatc agcgacatta ccgtctgcat cctcgatcgc
     9841 ccgcgccacg ccaaactcat cgaagaggtg cgcgcaacgg gcgcggcgct gcggctcatc
     9901 ggcgacggcg atatcgcagg cgtgattcac gtcaccaagc cgatggaaac cggcaccgac
     9961 atctatatgg gcatcggcgg cgcgcccgaa ggcgtgctgg cggcggcggc gctcgcctgc
    10021 atcggcggcc agatgcaggg gcggctcgtg gcttcgaacg agcagcaacg ctcgcgcgcc
    10081 cgcgccgccg gcatcctcga tctcaaccgc aagttccaca tccacgacat ggtgacgggc
    10141 gatctcgcct ttgccgccac cggcatcacc gacggatcgc tcctcgaagg cgtccgcttc
    10201 accaaggacg cgatctttac ccacactctg gtcatgcgcg cctcgacccg cacggtgcgc
    10261 tggatcaagg ccgagcatcc cgatatcagc aagttcgact gagggcttaa cggagaggcg
    10321 gggcgctccg cgaatcacct tacactccca cgccgagacg acagcgtgga tgacgtagcg
    10381 tgaccgcggc cctgaaattc caagccaatg agattgcgag cgccttcctc ggcgtggaac
    10441 gctcggcgcg cggctatcgc tgggtcgaac gcctggatcc cgcccgcgcg ctgaccgccg
    10501 ccgccatttc gcaagcccac tcgctccccg agcttatcgg ccgcgtgctt gccgcgcgcg
    10561 gcgccgacat ccagaccgct acccgctatc tcgatccgtc gctccgcacg cttcttcccg
    10621 atccggctcg ccttgaggac atggagaagg cggccgcgcg cttcgcttcc gcgatcgcgc
    10681 agaacgagcc gatcgccgtg ttcggcgatt acgatgtgga cggcgcggcg tccgtcgcgc
    10741 tggtggagcg cttcctgcgc gcgcacggcc aaagtgtcgc gacctacatt cccgaccggc
    10801 tcacggaagg ctacggccct tcgccggagt cgcttgccgg cctcgccaaa gacggggcgc
    10861 gtctcattct caccgtcgat tgcggcacga ccgccgagat cgccatcgct gccgcgaacg
    10921 ccgccggcgc cgaggtgatc gtcatcgacc atcaccaggc cgacgaggcc ctgccgcccg
    10981 cattcgccgt gctcaacccg aaccgacagg acgatctctc ggggcagggg catctcgccg
    11041 cggcgggcgt cgtattcctg ttcctggtgg cgacgacgcg cgcgcttcgc cgcgaaggct
    11101 tttacggcaa caggcccgag cccgatctcc tcgggcttct cgatctcgtg gcacttgcct
    11161 cggtatgcga cgtggtgccg ttgagggacg tgaaccgtgc tttcgtcgca cagggactca
    11221 aagtcttgcg cctcagaaag aatcaaggcc tgcgcgcgct cgccgatgcc gcgcggatcg
    11281 acgcggcgcc gacatgttat gcgctcggtt tcgtgctcgg gccccgcatc aatgccggcg
    11341 gaagggtcgg cgcatcgagt ctcggcgcga gactgcttgc gaccgacgac gacatcgagg
    11401 cgcgcgcgct tgcagccaag ctcgaagcgt tcaacgccga gcggaaggcc atagaagaac
    11461 gcatgctcga agaggcgttc gcgtccgcag agacatcgct tgccgccgat ccgggctcgc
    11521 cgttgctctt ccttgccgcc gcgggctggc acaaggggct gctcggtttg gtcgctggcc
    11581 gcgtcgccga gcgttttcgg ctcccggctt tcgtctcgtc gctcgagcct gacggtttcg
    11641 ccacgggttc ggcccgctcg attgcatctg tcgatctcgg ggccgcggtc aggagtgccc
    11701 tgcatgaagg gcttctcgcc aaaggcggcg gccacgccat ggcggcgggg ttcaggttcg
    11761 agcgccgcaa agaggagcgg ctcgtcgcat tcctaagggc tcggcttgcg gcgccggtgg
    11821 cgcaagcggg cacggtgcga gcgcttgcca tcgacggcgc gctctcggcc ggcggcgcca
    11881 acaaggacct catggatcag ctcgaccgcg ccggtcccta cgggccgggg caccccgagc
    11941 cgcgcttcgt cttcccggcg caccgtctcg cgcgcgtacg cagcatcaag gacgcgcacc
    12001 tccgctgcac gctgaaagcg cccgacggcg cgcggctgga ggcgtgcgcc tttcgcatcg
    12061 ccgacacgcc tctcgccaag ctcctcctcg acggggaggg gcagacggtc catgtcgcgg
    12121 gacatttgcg gcgcacgtct tggcagggcc gcgagagcgt cgagctcctg atcgaggacg
    12181 cggccgcgac ccaccgttcc aaccaatgac tcgtcatggc cgggcacccg ggtccggctt
    12241 cgccggcccg ggcgcaggcg aggagacgag caagacacat atcgccaagc acacctgccg
    12301 catcccctca tgctgaggag ggccggaagg cccgtctcga agcatgcacc cttacgcacc
    12361 tcgtcatgcc cggcttcatg ccgggcatcc acgatttggc aagtggaaaa agaggcaatc
    12421 gtggatggcc gggccaagcc cggccatgac gccactgagt taggggggcg acgcgcatcc
    12481 ttgcggcgat ttgcgaggcc ttctatatga cgaggctcgg tgcgccccct tcgtctaatg
    12541 gtcaggacgt caggttttca acctgaaaac aggagttcga tcctcctagg gggcgccagt
    12601 acccgctacc acttgccgat gttcggcatc gaggcccagg gctcttgctt cggcttcggc
    12661 ttgcccctct gcaacagctc gatcgagatg ttgtccggcg agcggatgaa ggccatgtgg
    12721 ccgtcgcgcg gcggccggtt gatgacgacg cccttcttca tcaggcggtc gcaggtcgcg
    12781 tagatatcgt cgaccgcgaa ggcgagatgg ccgaaatttc gaccctcgcc gtaagcttcc
    12841 ggatcccaat tataggtgag ctcgatctcg gcttctgcat cctcaggggc cgcgagatag
    12901 aacagggtat agcggccttg cgggttttcc atccggcgca cctctttcaa cccgagcttg
    12961 ttgcaataga agtcgagcga ttgatcgacg tcggagatgc gcaccatcgt gtgcagatac
    13021 ttcatgcttg tctctcttac gctgtagcga cgctgaggcg caccccttag cgcgccctgc
    13081 gcatctgttg caagcgcaac acgctccagt tttttccgat gcagattgtc ttccgattgt
    13141 tgcccggggt ccacagcgcg ccgcggatgc caattcgcgg tgcctgaagt gaaatttctc
    13201 tcgacggcag agccatttga gcgatcgcag catcaattgg accacgtttt ttgccaatgg
    13261 gatcgccaaa tggcgttttt ctgaaagatt cgctggccga tcagatggtt gcgctcctct
    13321 cttgcgggga ggtcgcgcaa atcccggggg attgtggaga aggaggaaac gtccggaaaa
    13381 ttccactggc ctcaaggggc ggcaatgcta tcttcgcaac gattgtgttc cggtcgattc
    13441 gtgtgtggcg cgtcgggtcg gccgcgtggg aagtgaagcg ggggaattgg ggcatggggg
    13501 cgaaagaacc gccactcgaa gagggccata cccaacgcga cgaggaagcc acagttgatg
    13561 tcttcgaagt gtcaggatcc atcaagtggt tcgatgcgtc aaagggttat ggattcatcg
    13621 ttccggacga agaccttccc gacgttctcc tgcatgtgac gtgcctgcgc cgcgacgggt
    13681 tccagacggc ctacgaagga gcgcgcgtcg tctgcgaggt gctgcgccgg ccgaagggtc
    13741 tccaggcctt tcgcattcgt gccatggacg attcgacggc gatccatccg tcgcaattgc
    13801 cgcagcgcac ccatgtcatt gtggtgccgg agagcgattg ggagcaggcc accgtcaagt
    13861 ggttcaaccg cttgcgcggc ttcggctttc tcacgcgcgg ggcccagagc ctcgacatct
    13921 tcgtgcatat ggagacgctg cgccgctgtg gcttcaccga gctcaggccc ggccagtcgc
    13981 tcctggtaaa gtatggccgc gggccgaaag gactgatggc ggcagagctc aaaccggaca
    14041 gcgcgcaagg tccttcgtct cactagcgca acggcgcatc ggccggctcg cccgcatcgt
    14101 ggcgggcttt ttgtttggcg gcgcgcttct tgcgcaggcg gtcgcgtttg ccgcgagcgc
    14161 gggcacgatc gtgctcaaga cggagacggg cgaccacagt ttcgacatcg aagtgatgac
    14221 caccgatcgt gagcgcgcgc acgggctcat gtatcgccgc tcgctgccgg aaagaagcgg
    14281 gatgctgttc ctctacgatc ggcctcaagg cgccaccatg tggatgaaga acacgttcat
    14341 cccgctcgac atggtgttca tcgcgccggg cgggaccgtg caccggatcg agaggaatgc
    14401 cgagcctttc tccaccgagg tgatctcctc cgagggtgcc atcatcgccg ttctcgagtt
    14461 gaatgcgggc gaggccgacc ggatcgcgct gaagcgcggc gataaagtga tctatccggg
    14521 cctcggcgag agtccctagg caaaaacctc ccccctgcct tcgccgaagc gaagctccgg
    14581 cttcgcgcag gcaggtcgtg gggagggtcg gcgcgcttta tctttgcgct atcgcgctgc
    14641 tacttgagcg caaagcgcgc cgggtggggg gcatgcgctt caaccggtaa accctcatcc
    14701 tggggagccg ccgaaggcgg cgtctcaaag gatgcgttcc gatcgccgtg cttcgagacg
    14761 cgctccttgg gagcgcacct cagcatgggg ggagaggaag agggttctat taggggtcgc
    14821 ttagggctta agggctagcc tcggaggcgc gcgcgactta tgtatcttcc cgcgggtcca
    14881 aaggccgttt ccggccgggg tcccgcgcga gagcgattga tcggggcata gcgcagtctg
    14941 gtagcgcacc tgctttggga gcagggggtc gcaagttcga atcttgctgc cccgaccatc
    15001 tcccggctct cggcattttt ggaggaggcc gcatggccgc acgaatttac aagccggcga
    15061 agacggcgat gcagcagggc atggcgcccc gcgactgggt gctggaatac gagccggacg
    15121 aggcacaggt catcgagccg ttgatgggct ggaccagctc cttcgacacg aagtcgcagg
    15181 tgcggctccg cttcaccacc aaggaggaag cggtggcgtt cgccgtgcgc cacggcatcg
    15241 ccttccgtct cgaagagccg aaggagacgg agctcagacc gaaatcctat gccgagaatt
    15301 tcaagttcgg ccgccccgac cgctggacgc actgacgcgc cgcttcgggt cttttcgcgt
    15361 tatagtccaa gccggatttg caaaacggtc ccgtagctca agggatagag catctgcctt
    15421 ctaagcagag ggttcaaggt tcgaatcctt gcgggaccgc cacttaattt gggggagagc
    15481 tccatgctcg acggtagcga gaagtggatt ggcccttttc aagtacggaa gatgctcgag
    15541 cactgtttgg acgattcagt tgtgcccaaa gcactggagt ccggtagcgc ctaccttgtg
    15601 acgcggaagg attggcggac tgctcccacc catgaaagca taccgctgta cgtcggtgga
    15661 aacacaggcg aagcaccgcg ctttagaacg cgcgtcggtg acttattggc ggacgccttc
//
LOCUS       DASUQP010000121         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1966937, whole genome shotgun sequence.
ACCESSION   DASUQP010000121 DASUQP010000000
VERSION     DASUQP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966937"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1300)
                     /locus_tag="VFY74_05455"
     CDS             complement(68..1300)
                     /locus_tag="VFY74_05455"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5931182.1"
                     /translation="MISVPAEDPVHALVPHTLTEALAGSAEGPLAGLSFMVKDLFAVA
                     GRKVGNGHPTHYAQSAPASKTAPAIIRLLEAGASLTDITICDEFFYSVLGSNAHYGQP
                     VNPRAPKRVTGGSSCGAAAAVAAAMCDFALGSDTGGSVRVPASFTGLYGLRPSFGRIA
                     TEGATPMAPSYDAIGFLARDAELFRNVGRVLLEGERVAAKVDRLILAQDLFVHEEAEA
                     EEALWSVLAKIGPALPVPEHRDIAGDELEDWRNAFRLIQGFEIQSTLLPFIQSNGVSL
                     GPGIKERFAMAAGITFDEAEAARRLRGEIAERLHAMARPGTLIVLPTTPTLPPERGIP
                     DGAAFAAFRTRILGYTCLAGHACLPQISIPAAEAAGCPIGLSFIGWKGGDEAMLDLAV
                     TIANTFPQASSANQGVHL"
     gene            1462..2004
                     /locus_tag="VFY74_05460"
                     /pseudo
     CDS             1462..2004
                     /locus_tag="VFY74_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2117..2608
                     /locus_tag="VFY74_05465"
     CDS             2117..2608
                     /locus_tag="VFY74_05465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5931183.1"
                     /translation="MESGLALAWAPAVIAVMSLVFAASAYPAGRLQDRIGGRLLLLLG
                     LATLIAADLVLAYAPSILAVFVGIGLWGLHMGLTQGVLAALIAATAPERLRGTAFGLF
                     GLIAGLATFIASVLAGLLWDRVGAGATFLAGAAFAAVALLAFLPITRIPGGAGGSGAA
                     PPI"
     gene            2625..3485
                     /gene="dapF"
                     /locus_tag="VFY74_05470"
     CDS             2625..3485
                     /gene="dapF"
                     /locus_tag="VFY74_05470"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5931184.1"
                     /translation="MQAETIQFRKMNGLGNDFVVLDARQRALPLSSDAVRAIADREEG
                     IGCDQIVALEPSAKADVFMRIWNADGGEVGACGNAARCVAALVAAERDVANVSIETES
                     GVIGASVNADRSVTIDMGAPRFAWNEIPLSKPFDDTRAIDIGPINDPALVSPSAVNVG
                     NPHCLFFVDDVEAHDLARLGPALEHHPLFPERANISLVQVLGPSHLKLRTWERGSGLT
                     RACGTAACAAAVAAARRELSGRKVSVSLPGGDLRIEWRETDGHILMTGPYALDYEGTL
                     PAALSQPARV"
BASE COUNT          550 a         1167 c         1228 g          652 t
ORIGIN      
        1 agggccgacg tcccgatgac ctcgaatgtg atcgcggcgg caaggtagag ataaggcaaa
       61 gcgagagtca cagatgaact ccttggtttg cggaacttgc ttgcgggaat gtgttggcaa
      121 ttgtgacggc gagatctaac atcgcctcgt cgcccccttt ccagccgatg aaagagaggc
      181 cgatgggaca gccggctgcc tcggcggcgg gaatggaaat ctgcggcagg caggcgtgtc
      241 cggcgaggca ggtatagccg aggatccggg tgcggaacgc ggcgaaggcc gcgccatcgg
      301 ggatgccgcg ctcaggcggc agggtcggtg tcgtcggcag cacgatcagc gttccgggcc
      361 ttgccatcgc gtggaggcgt tcggcgatct cgccgcgcag gcgacgcgcc gcttcggcct
      421 cgtcgaaggt gatcccagcc gccatcgcga accgctcctt gatgccgggt ccgagactaa
      481 ccccgttcga ctgaatgaac ggtaacagtg tcgattgaat ctcgaaaccc tggatcaggc
      541 gaaaggcatt gcgccaatct tcgagctcgt cgccagcaat gtcccggtgc tctggaacag
      601 gcagcgccgg tccgatcttc gcaagaacgc tccacagcgc ctcctcggct tccgcctctt
      661 catggacgaa caggtcctgg gcaaggatca ggcggtcgac ttttgccgcg acgcgctctc
      721 cttcgagcag cacgcgtcca acgttgcgga acagctcggc gtcgcgcgcg agaaagccga
      781 tcgcatcgta gctcggcgcc atcggcgtcg cgccctcggt cgcgatgcgg ccgaagcttg
      841 gcctgagacc gtagagcccg gtgaaggacg caggcaccct gaccgagccg ccggtgtcgc
      901 tgccgagcgc gaagtcgcac attgccgccg cgacggccgc ggccgcgccg caagaagacc
      961 cgccggtcac gcgcttcggc gcgcgcggat tgacgggctg gccgtaatga gcgttagagc
     1021 cgagcacgct gtagaaaaac tcgtcgcaga tggtgatgtc ggtcaatgac gccccggcct
     1081 ccagcagccg gatgatcgcc ggcgcggtct ttgaggccgg agctgattgc gcgtaatggg
     1141 tcggatgccc gttcccgact tttcggcccg cgacggcgaa caggtccttc accatgaacg
     1201 agagacccgc gagcggcccc tcggcgcttc cggcaagcgc ctcggtcaga gtatgaggga
     1261 cgagcgcgtg aacagggtct tcggccggaa cggagatcat gtccagtggc gacgcctcgg
     1321 tcagtagagc gacaggtcgc cttctatatc acaggcgatt gagcgatcgc cgggacgaag
     1381 gagcagtccg tgagagcggg agaagcgagc ggacagaacc tgccgcgagc ggtgtgggcg
     1441 ctcggcctga cctcgttgct catggatacc tcgtcggagc tcatccacgg actgctcccg
     1501 gtttttctcg tcgtcacgct cggcgcgagc gcgaccgcgc tcggtgtggt cgaagggatc
     1561 gccgaggcga cggcacaggt cacgcgcgtg ttttccggct ggctctccga tgcgctcggg
     1621 cggcgcaagg cactcgccgt gctcggctat agcctcgcgg cggcgacgaa gccgctgttc
     1681 ccgctcgcga actcgatcgg gctcgtgctc gtggcgcgtt tcctcgatcg catcggcaag
     1741 ggaatccgcg gcgcgccccg cgatgcgctg gtcgcggacc tcacgccctc cggcatgagg
     1801 ggcgcggcgt tcgggcttcg ccagtcgctc gacacgatcg gcgccgcgct cgggccggcc
     1861 ttggcgatcg gcttgatgca tctgttcgac gacgacattc gcgccgtgct gtggttcgcg
     1921 gtgatccccg cggtcttggc cgtcgccgtc ctcgtctttg gcgtcgatga gccggggacc
     1981 gcgcaagcga aagcgcgggc gccgctaagg cttgcggagg tcggcgcttt gggctccgcc
     2041 tattggcttg tcgccgccgc aggcgccgtc ttcgccttgg cacgtttcag cgaggctttt
     2101 ctcgtcatcc gcgcgattgg agagcgggct tgcgctcgcc tgggcgcctg cggtcatcgc
     2161 cgtgatgagc ctcgtctttg cggcaagcgc ctatccggcg ggacggctcc aggacaggat
     2221 cggcggccgc ctgctcctcc tcttgggcct cgccacgctc atcgccgcag acctcgtcct
     2281 ggcctacgcc ccttcgatcc ttgccgtctt tgtcggcatt gggctttggg gcctgcatat
     2341 gggcctcacg caaggggtgc tggcggcgtt gatcgcagca acagcgccgg agcgcctgcg
     2401 cggcacggcc ttcgggctgt tcggtctcat cgccggactt gccaccttca tcgcgagcgt
     2461 gctcgccggg ctcctgtggg acagggtcgg ggcgggagcg acgttcctcg ccggggctgc
     2521 cttcgcggcg gtcgccctct tggcgttcct accgattacg cgaataccgg gcggcgctgg
     2581 cggaagcggg gccgcgcccc ctatatagcg gtacgcgagg cgccatgcag gctgagacga
     2641 tacagttccg caagatgaac gggctcggca acgacttcgt cgtgctcgat gcacgtcagc
     2701 gcgcgcttcc gctctcgtct gacgcggtcc gtgccattgc cgaccgcgaa gagggcatcg
     2761 gctgcgacca gatcgtggca ctcgagcctt cggcaaaggc cgacgtgttc atgcgcatct
     2821 ggaacgccga tggcggcgag gtcggcgctt gcggcaacgc tgcgcgctgc gtggcggcgc
     2881 tggtcgcggc cgagcgcgac gttgcgaatg tcagcatcga gaccgagagc ggggtcatcg
     2941 gcgcttcggt taatgccgat cgcagcgtca ccatcgacat gggcgcgcct cgttttgcct
     3001 ggaacgaaat tccactctcg aaaccgttcg atgatacgag ggcgatcgat atcggtccga
     3061 taaacgatcc tgcgctcgtt tcgccgtcgg ctgtcaatgt cggcaatccg cattgcctgt
     3121 tcttcgtcga tgacgtcgag gcgcacgacc tcgcccggct tggacccgcg ctcgaacatc
     3181 atccgctctt ccccgagcgc gccaatatct cgctcgtcca ggttctcggc ccctcgcatt
     3241 tgaagctgcg cacctgggag cggggctcag gcctgacgcg ggcctgcggc acggccgctt
     3301 gcgccgccgc cgtcgccgcg gcgcgacgcg agctttcagg gcgcaaggta agcgtaagcc
     3361 tgccgggcgg cgatcttcgc attgagtggc gcgagacgga cggccatatc ctgatgaccg
     3421 gtccttatgc cctcgactat gagggcacgc tgcccgcagc gctgtctcag ccggcccgtg
     3481 tttgaggttc gtcattccgg cgtaagcccg aattttcctc tcccattgag ggcgagggct
     3541 tttccgcccg gcgcgcgtag cgcgacgcaa agcgggaagg gtgagggcga aatcttt
//
LOCUS       DASUQP010000122         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1693579, whole genome shotgun sequence.
ACCESSION   DASUQP010000122 DASUQP010000000
VERSION     DASUQP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693579"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..951
                     /locus_tag="VFY74_05475"
     CDS             328..951
                     /locus_tag="VFY74_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931185.1"
                     /translation="MRVVIDPERSRDLREIELRRHLQMMARSIDRAEAGFTLTMILRS
                     AASDPAQALVGVKGALQRAGLRAKVILTRVDPEDELRQLFASLSELAPQVPAPELIRW
                     ARNPRLLEAHEQVVYGKTMCWSGDAMRRDADRRNALTLFDEESADKVHAGRLAFAALW
                     AASSPVPERHLTGSLTSRPYGAFEQPVDAPVLSPTAPGPQGWPLLRH"
     gene            1029..1433
                     /locus_tag="VFY74_05480"
     CDS             1029..1433
                     /locus_tag="VFY74_05480"
                     /inference="COORDINATES: protein motif:HMM:NF020395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family c-type lysozyme inhibitor"
                     /protein_id="HEX5931186.1"
                     /translation="MHAVLRVFLLAASCFSAFAADAPKGPNLSDLMKQPAHRAAWYGM
                     FAGETPPSWVEDYAKTLDGPPTPSIAVAANGETYTLAFTCKANECGDNQLFVLFSPGG
                     AKAWGLLIAGSEQIWFGAPDEAIRKAILSGLD"
     gene            1575..2414
                     /locus_tag="VFY74_05485"
     CDS             1575..2414
                     /locus_tag="VFY74_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931187.1"
                     /translation="MNRRKFLVSSASAGLAAMTAGTTFAAAPVPAPEPMLHRCGTPPP
                     TPSEDIQANRVMDALREHRVTFRGKTVIPIRFHIIHVGAQGYLPHRRLKAQVALINRI
                     FAPAQIAFRIAEARLHENKAWFTHEPGSRAEIAMKTELGQDTAYALNIYTAEPGGGLS
                     GYATFPWSQAGAPQRDGIVLHHASLPDAADSSVNESSSFDSGMAAVHQIGHWAGGFDH
                     SVEDGCEASGDATESLEDTCLTPFTPQQYERVRDMVGYYRYKMSPLASRSALLAQIRE
                     SIE"
     gene            2435..2575
                     /locus_tag="VFY74_05490"
     CDS             2435..2575
                     /locus_tag="VFY74_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931188.1"
                     /translation="MKKTPDKRRPKPQKETRKPKISRNERLAQQTRKLLGKHYASKYR
                     VR"
     gene            2596..3594
                     /locus_tag="VFY74_05495"
     CDS             2596..3594
                     /locus_tag="VFY74_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5931189.1"
                     /translation="MQAMLFDAPGKPLRAAELEVPSPGPAQILIEVLACGVCRTDLHI
                     FDGELPHPKLPLILGHEIVGRVARLGDGAEGFAVGERVGVPWLGGTDGTCRYCREGRE
                     NLCDNPRFTGYQIDGGYAGYTIADARYVFKLPEAYSDAEAAPLLCAGLIGYRSLKLAG
                     TPLAGEVSRLGIYGLGAAAHIVAQVAKAQGREVFAFTRPGDRPAQDFALSIGAVWARG
                     SDEKPPEALDAAILFAPVGALVPEALRAVRKGGVVVCGGIHMSDIPSFPYDILWEERV
                     LRSVANLTRDDAREFLALAPQVPVNTHVSPMPLRAVNEALERLRRGELTGAAVLIP"
     gene            complement(3613..4308)
                     /locus_tag="VFY74_05500"
     CDS             complement(3613..4308)
                     /locus_tag="VFY74_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5931190.1"
                     /translation="MRRAAGVFKTFGLASALLLAPGLAKATDLPPMSPVAQPVIEEPP
                     TWQGFYLGGNLGAAFDPYNLSIKDLSAAQDLTLKFSNDTEFIGGVHGGYNWQAGSLLL
                     GVEGDIAFASDIDYLASVRGRVGWASPNWLVYGTAGVAFIDTSTNFTVVSADNGPFGF
                     NLGNTDTGFVGGGGIEYRLGENLSLGVEGLYYDFGSDTQNLTAGAGEPFVLKDELDFA
                     VARGRLTYHFNGF"
BASE COUNT          771 a         1464 c         1425 g          779 t
ORIGIN      
        1 agatcggggc atgccgccgt ggcactggcg gcgctggggc tgtgtagagg gtcctagtcg
       61 gttcgagtaa cgcgtaatgc tccgtcttgc gtaccggctc ggcacgaaca ccgagtcggg
      121 tcagtttgag tttgatgcag ccgccctaag cgcggtctcg ctccgtggat tcgcatctgc
      181 gccgtttgca gtcgggcgca ccctggcggt tccgaaacgg cacagccctc aaatcttcct
      241 taaccctgtt ctggcaattc ttagcgaggc gagaggcggc accatagcgt ctgccctcgg
      301 ccgattctgc gagatgcgag ggggaatatg cgcgttgtga tcgatccgga gcgttcgaga
      361 gacctaagag agatcgagct ccgccggcat ctgcagatga tggcgcgatc gatcgaccgc
      421 gccgaagcgg gcttcacgct taccatgatc ctgcgctcgg ccgcgtccga cccggcccag
      481 gccctggtcg gggtcaaggg cgcattgcag cgcgccggcc ttcgcgccaa ggtgatcctg
      541 acgcgggtcg atcccgagga cgagttgcgg cagttgttcg cgagcctctc ggagcttgcg
      601 ccgcaggttc cggctcccga gctgatccgc tgggcgcgca atccacgcct cctcgaagcg
      661 cacgaacagg tcgtttacgg caagaccatg tgctggtcgg gcgacgccat gcgccgcgat
      721 gccgacagac gcaatgcttt gaccctgttc gacgaagagt cggccgataa ggtgcatgcc
      781 gggcggcttg ccttcgccgc gttgtgggcc gcatcttcgc ccgtgcctga gcggcatctg
      841 accggctctc tcacgagccg tccctacggc gccttcgagc agcccgtcga tgcgccggtg
      901 ctctcgccca ccgcgccggg cccgcaaggc tggcctctcc ttcggcacta gcctcttttt
      961 tcggatataa cctcctcgct gacggcaggg tgcggttcac cgcagccccc gccccgacgg
     1021 agtgatcgat gcacgcagtc ctgcgcgtct tcctgctcgc cgcaagctgc ttttccgctt
     1081 tcgccgccga tgcgcccaaa ggtcccaatc tttccgacct gatgaagcag ccggcgcatc
     1141 gcgccgcctg gtacggcatg ttcgcgggcg agaccccgcc gtcatgggtc gaggactatg
     1201 ccaagaccct tgacgggccg ccgacgccct cgatcgcggt tgcggcgaac ggcgagacct
     1261 acacgctcgc cttcacctgc aaggcgaatg aatgcggcga caaccagctt ttcgtcctgt
     1321 tctcgccggg cggggcgaag gcctggggcc tgctgatcgc cgggagcgag cagatatggt
     1381 tcggcgctcc ggacgaggcc attcggaaag cgatcctgag cgggctcgat tagcggcgag
     1441 aagaggtccg accgaaggcg tggcacagcc ggctcgcgca atctattctt gccgatcgga
     1501 caaaaagtgg ttcggtttgg atgagctcaa tcggctacgc ctctgcaagg aatgaacgtc
     1561 aaggagcgca agtcgtgaac cggcggaaat tcctcgtctc ttctgcgtcg gccggtttgg
     1621 cagccatgac ggcaggcaca accttcgcgg cggcgcccgt gcccgcgcct gagccgatgc
     1681 tccatcgctg cggaacgccg ccgccgacgc cgagcgaaga cattcaggcc aatcgcgtga
     1741 tggatgcgct cagagagcac cgcgtgacct tccgcggcaa gacggtgatc ccgatccgct
     1801 tccacatcat ccacgtgggg gcgcaaggct atctgccgca ccgccggctc aaggcccaag
     1861 tggcgctcat caatcgtatc ttcgcgcccg cccagatcgc cttcagaatc gccgaggcga
     1921 gactgcacga gaacaaggcc tggttcaccc atgagccggg aagccgcgcc gagatcgcca
     1981 tgaaaaccga gcttgggcaa gacacggcgt acgcgctcaa tatctacacg gcggagccgg
     2041 gcggcgggct ctcgggctat gccaccttcc cctggtcgca ggcgggagcc ccccagcgcg
     2101 acggcatcgt cctccatcac gcgagcctgc ccgacgccgc ggactcctcg gtgaatgagt
     2161 cctcgtcatt cgattccggc atggcggccg tgcatcagat cggtcattgg gccggcgggt
     2221 tcgatcacag cgttgaggat ggatgcgagg cgtccggcga cgccacagaa tcactcgaag
     2281 acacctgcct gactccgttc acgccgcaac aatatgagcg ggtgagggat atggtgggct
     2341 attaccgtta taagatgagc cctctggcca gccggtccgc gctgctcgcg cagatccgag
     2401 agagcatcga gtaggcggcg tgagggtcaa aaggatgaag aagacccctg acaagcgaag
     2461 gcccaaaccg caaaaggaaa ccaggaagcc gaaaatctcg cgcaacgaac gcttggcaca
     2521 gcagacgcgc aagctgttgg gcaagcacta cgcaagcaaa taccgcgtgc ggtagcttcg
     2581 atgcctcctt ccgtcatgca ggcgatgctg ttcgatgcgc caggcaagcc gctccgtgcc
     2641 gctgaattgg aggttccgag tccgggaccg gcacagatcc tgatcgaggt tttggcttgc
     2701 ggcgtttgcc gcaccgacct tcacatcttc gatggcgagc ttccgcatcc aaagctgccg
     2761 ctcattctcg gccatgagat cgtgggccgc gtggcgaggc tcggcgacgg cgctgagggc
     2821 ttcgccgtcg gcgaacgcgt cggcgtgccg tggctcggtg ggaccgacgg cacctgtcgc
     2881 tactgccgcg aaggccgtga gaatctctgc gacaatccgc gcttcaccgg ctatcagatc
     2941 gacggcggct atgccggcta tacgatcgcc gatgcgcgct acgtcttcaa gctgcctgaa
     3001 gcctacagcg acgccgaggc ggcgccgctg ctctgcgccg ggctgatcgg ctaccgttcg
     3061 ctcaagctcg ccggcacgcc gctcgccgga gaggtgtcgc gtctcggcat ctatggcctc
     3121 ggtgccgccg cccatatcgt ggcgcaagtg gccaaggccc aaggccgcga ggtcttcgcc
     3181 ttcacgcggc ctggcgaccg gcccgcgcag gattttgccc tttcgattgg cgcggtttgg
     3241 gcgcgaggct ccgacgaaaa gcccccggaa gcgctcgacg ccgccatcct gtttgcgccc
     3301 gtcggcgccc tggtgccgga ggctcttaga gcggtgcgca agggcggcgt cgtggtctgc
     3361 ggcggcatcc atatgagcga catcccgagc ttcccgtatg acattctctg ggaggagcgc
     3421 gtgttgcgct cggtcgccaa tctcactcgc gacgatgcgc gcgagtttct ggcgctcgcg
     3481 ccgcaagtgc cggtcaacac gcacgtcagc cccatgccgc tccgcgccgt caacgaggcg
     3541 ctcgagcgcc tgcgccgggg cgagctgacc ggcgccgccg tgctcattcc ctgacgccgc
     3601 ttttggggac ggttagaagc cgttgaagtg gtaggtcagc ctgcctcggg cgaccgcaaa
     3661 atcgagctcg tccttcaaga cgaacggctc gcccgcgccg gcggtcaagt tctgcgtgtc
     3721 gctgccgaaa tcgtaataga gcccctcgac gccgagactg agattctcgc ccaggcgata
     3781 ctcgatcccg ccaccgccga caaaacccgt gtcggtgttg ccgagattga atccgaacgg
     3841 cccgttatcg gcggagacca cggtgaaatt ggtgctcgta tcgatgaacg ccacgccggc
     3901 ggtgccgtag accagccaat tgggggaggc ccagccgacc cggccgcgga cgctggcgag
     3961 gtaatcgatg tcgctggcga aggcgatatc gccttcgacg ccaaggagaa ggctgcccgc
     4021 ctgccagtta tagccgccat gcacgccgcc gatgaactcg gtgtcgttcg agaacttcag
     4081 cgtgaggtcc tgtgccgcgc tcagatcctt gatgctgaga ttgtagggat cgaaagcggc
     4141 accgagattg cctccgaggt aaaagccctg ccacgtgggc ggctcctcga tgaccggctg
     4201 cgccacagga ctcatgggcg gcaagtcggt cgccttggcg agaccgggcg cgagcagcaa
     4261 agcgctcgcc aagccgaacg tcttgaatac tcccgcagca cgtcgcatcg ctatctccct
     4321 ccggggcgga tcgcgtggca tctccggcca gcattgctcc acctcgctgc gggataacct
     4381 ccgcgattaa tcgagcgaaa ctgcggccgc gcgcgccaca aagtcgcccc tgatgcggc
//
LOCUS       DASUQP010000123        12538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1829651, whole genome shotgun sequence.
ACCESSION   DASUQP010000123 DASUQP010000000
VERSION     DASUQP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12538
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829651"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VFY74_05505"
     CDS             <1..1100
                     /locus_tag="VFY74_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5931191.1"
                     /translation="LATYVGWRLHGTLGALAAGLLFVLPGALIVLGLSIAYAYYGQVP
                     AVEAAFVGVKAAVLVIVIEALVRVAKRALRGPHDVGIAAAAFVAIFFFAAPFPLIIAT
                     AAAIGFLLARAPPRPAAAMPANSEAIPLSRTLKTAGLWLAIWILPLLLIAAIFGRSHV
                     TTDISLFFSKLAVVTFGGAYSVLAYMAQQAVETYGWLTAGEMLDGLGLAETTPGPLIL
                     VTEFVGFLAGFRQGGEPKLAFGLLGAAVTLWATFAPCFLWIFAGAPYVERLSANPRLA
                     GALAGVTAAVVGVILNLSLWFALHVVFGSVTAVWHGPVQLWTPDLASLNVEALTLAIL
                     AAILLFGFHFGIVSTLAIAAAAALAWSQLGF"
     gene            complement(1137..1442)
                     /locus_tag="VFY74_05510"
     CDS             complement(1137..1442)
                     /locus_tag="VFY74_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931192.1"
                     /translation="MNIRSLILAFAAASFAILVGKAAPASAAPGLVATPEAGAALVQE
                     AGYYKRRWRRNGTYVRAPFADVDARRETWVAAPFARVYNGRWGTWVRAPFVNLWVPR"
     gene            complement(1525..2496)
                     /locus_tag="VFY74_05515"
     CDS             complement(1525..2496)
                     /locus_tag="VFY74_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX5931193.1"
                     /translation="MLTIYENRSGALERQKGKPRITEEALWVDLLNPTPEDEKKVERA
                     LKFNVPTREEQQEIEASSRLYQENGAYFMTATLLYHSDQGEPRTTPVTFILAGQRLVT
                     VRYAQPRAFSIYVERCNRSEAELKSGSAVLIGLLETIIDRLADFIERLQAEVEGLSHS
                     IFEIKGGAASRQRRFDVLLRAIGREGEITSKARESAHSLGRLLTFLIHAANERKEVKP
                     LQARIRTAARDVASLTDHATFLSGKIIFLLDATLGMINIQQADIIKIFSVAAVVFLPP
                     TLIASIYGMNFQFMPELSWWVGYPLALALMILAAVVPYLYFKRRGWL"
     gene            complement(2616..3542)
                     /locus_tag="VFY74_05520"
     CDS             complement(2616..3542)
                     /locus_tag="VFY74_05520"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5931194.1"
                     /translation="MLRRLRAASAAASFLLAASLLAASSAAAENAASSVETGVKEAGV
                     ALAGPKPFVDIVCPIMQQQAESRGLPPMPFVRLIWKESRFNPQAVSPKGAQGIAQFMP
                     GTAEERGLQDPFEPKSAIVHSASFLADLHREFGNFGLAAAAYNAGAERVRAWLSGSSG
                     LPWETQNYVQFITGRAAEEWKLEDTELPEPLRTAGAAVLESCRALAPLVVRAVYETEP
                     LTASGAWRPWGVHVSTSFSKAKALAQFGRLKRVHQALLGDREPFVLPDRNLSRGRRSL
                     YMVQIGADSRADAEKLCKLLRSQGGACIVQKN"
     gene            complement(3665..4252)
                     /locus_tag="VFY74_05525"
     CDS             complement(3665..4252)
                     /locus_tag="VFY74_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5931195.1"
                     /translation="MAIGRPRAFDADRALDRALDVFWRKGYEGATICDLTEAMGINPP
                     SLYAAFGNKEGLFRKALDRYWRLRTSLLDEALAQPTARRVAERLLHGWAKFLSDPCHP
                     KGCLTVHGALVSGEEADCIRGELEKRRATSQAGIRERLKRAKREGDLSADADPPALAR
                     FLATVMEGMAVQAASGASRKELDRVAATALRAFDA"
     gene            4402..5100
                     /locus_tag="VFY74_05530"
     CDS             4402..5100
                     /locus_tag="VFY74_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX5931196.1"
                     /translation="MIERRPFEALGGADHGWLKAKHHFSFARYYDPERMGHGALRVWN
                     DDEIAANTGFPPHPHADMEIITYVRAGAITHEDSLGNKGRTEAGDVQVMSAGSGVRHA
                     EYNRESVPTTLFQIWIETGTRGGAPSWGTKPFPKTGRSGRFVALASGFAGDTDALPIR
                     AEARVLGATLKSGESAAYEFAPGRHAYLVPAKGNIEVNGVRIDTRDGAAITGESVLVV
                     KALDDAEIVLVDAA"
     gene            complement(5133..5381)
                     /locus_tag="VFY74_05535"
     CDS             complement(5133..5381)
                     /locus_tag="VFY74_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX5931197.1"
                     /translation="MAKLVYRIVKHEDGWAYKVRGTFSETFPSHDEALAAARTAAGEQ
                     RVPGETVGIQYETADGVWREEVDPGTDRPQTVVEDEEE"
     gene            complement(5443..5874)
                     /locus_tag="VFY74_05540"
     CDS             complement(5443..5874)
                     /locus_tag="VFY74_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5931198.1"
                     /translation="MVQSYVLWALLGMAGYSLMTLFVKLAERSGAASTHMVLAVTTTI
                     VPIFAIAVVALRGELKPLLLELERPPLLWAIAGGIALTIAVNALFHALSLGPANVVVP
                     IYGMFIVGGSLLGVLFLGEPMTWNKIAGLVAAVIGVILIAL"
     gene            complement(6122..8068)
                     /locus_tag="VFY74_05545"
     CDS             complement(6122..8068)
                     /locus_tag="VFY74_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931199.1"
                     /translation="MTAVAIAALLIACTSIVFPSRAADPSKLCENEAGLTMLSSPIAP
                     WKGAALRVVFAAEKPLEGELSLIAPDGKVAAASRARTGGPPYVWFAEVAAPEAGTWQA
                     KLAREGAAPECGNVSREITVQRKEPPRPRASKSVWPINNTWNRATENLYSAWIEKLFD
                     APLEEAPSWKALHEVLRDPSRNILFDHLGLREDQKGHVIQPDCADVPYFLRAYFAFKM
                     GLPFGYAKCTRGDGGRPPRCQQWWNIVKEEPPPDPLLVQQEQTARMSAGVFGLFGPAA
                     PPPRVTRVARVPRKPQGLVPGFGYYLRTTLADAVHSGNGRTTADDNDTDYYPVPLTTE
                     TLRPGTVYADPYGHILVLVKRVPQSGDGAGVFLAIDGQPDGTVARKRYWRGNFLFAQD
                     PALGGPGFKRFRPIVREKNGTLRRLTNDEIANNADYGDFSLDQSKLQVEDFYDRMDDV
                     MSPAPLDPKRAMTEAITALEEQVKARVTSVENGRKYQVEGGREVAMPDGASIFETTGA
                     WEDFATPSRDLRLLIAMDVVRGFPDRVARRPDRYAMPKEKNVAEVKTELESMLASELA
                     ARTFSYTRTDGSQWTLALKDALDRMPALEMAYNVNDCVELRWGAAEGSEEATPCKRRA
                     PQSQRDKMTEYRAWFSGRRRPPRG"
     gene            8512..9564
                     /locus_tag="VFY74_05550"
     CDS             8512..9564
                     /locus_tag="VFY74_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX5931200.1"
                     /translation="MNQDVSTNTPQRAKGRAATYRWVALLVVLAATIGGVLLYWRYAE
                     LYPSTDNAYTGANIVRVAPQVSGSVANVYVRDDDKVAAGDPLFDIDSTLYDAALRNAR
                     AQFDAAASAAGTAADALKGAATKLEEKRVALEDAINKYREAETAQTEGEAPSAAFTDA
                     LKAWHDAVGAYNAAAGEFDAEQDKNLTVTTPTVQLRAASAALDKATHERVRTHVTAPA
                     SGWVSNVNLRPGSVVQAGTPALALVEDGNWWVDANFKETDLGRVKPGQKARIKLDLYP
                     GVMLDGIVESISAGSGSTFSVLPPENATGNWVKVTQRFPVRIRITSEPDPDRPLRLGA
                     SATVRIDTTEPAQGGK"
     gene            9561..11147
                     /locus_tag="VFY74_05555"
     CDS             9561..11147
                     /locus_tag="VFY74_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5931201.1"
                     /translation="MSPAKRVGGKGGKRQPSPEPPRPSLAELHPERPPIPLVIAVVLT
                     AVLEVLDITIVNVSIPNMLGAFAATPDQITWVLTSYMVAAAVVMPLTGYLSTWLGRRR
                     LLLLAITGFIVSSALCGMAWNLESMVLFRLAQGVFGAPLVPLSQAILLDAFPRERHGQ
                     ALAIFGLGIMVAPVIGPALGGYLTETYSWRMVFYINIPIGLVALLLSVGYLPRRPVAE
                     IKTDWVGMGLLVLAVGALQYVLDQGQTRDWLNSKAIVAGSIIAIFAGAAFFIRGWNRA
                     GNIIDLSLLKDRNFVAGTLAITAYGISLFGWMAIMPLFSQRLLDYPPATAGTLFIPRG
                     IVSAITLAITGSILIRLFDARHLVIAGLVMTALGTLPMAYFSLYVDYWGIATPGMIAG
                     IGSGLFFVPLTAVAFASIPREQYDEAAGVYALMRGIGGSAGIAIVSWLFVRQAHIHFN
                     ELTAHITPYNSDLVPYLGQHGLSPYSPEAAPLVVHEIARQAQMLAFNDIFWFIAMVTF
                     AIIPLIFLMKRPERVSLVPA"
     gene            complement(11248..11616)
                     /locus_tag="VFY74_05560"
     CDS             complement(11248..11616)
                     /locus_tag="VFY74_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931202.1"
                     /translation="MRSYMRVLGLSLIVAALCTVQGAPANAVCIGFSGTADGFDKVTA
                     VTRAQAAVAQAITDYKQQKRIRAVSVSPMRATPQPYWRRSVAQELFHKPDIVKSDSYT
                     TCWSGVVSPYVCTSGAKACW"
     gene            11825..>12538
                     /locus_tag="VFY74_05565"
     CDS             11825..>12538
                     /locus_tag="VFY74_05565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5931203.1"
                     /translation="MASKLVVVTGASSGIGEATATRYGASGVDVVLLARSADRLDAVA
                     KSIRAAGGNATTYPVDLADSRATEETAARIKRDLGTPDILVNNAGAGPWLPMAETTAE
                     EALRMIEVPYLAGFNLTRTFLPAMLARRSGAIAFITSPASYLAWPRASAYIAARRAVA
                     GLAETLQSELKGTGVAVTLVVLGTVETPYWQHNPGSRENMPKTNPRLVPVLTPDEAAS
                     AIVDGIAQGRAFVVKPGIYR"
BASE COUNT         2145 a         4200 c         3964 g         2229 t
ORIGIN      
        1 agctcgccac ctatgtcgga tggcggcttc acggcacgct tggcgctctc gccgccggtc
       61 ttcttttcgt gctgcccggc gccttgatcg tgctcgggct cagcatcgcc tacgcctatt
      121 acggccaagt gccggcggtg gaggcggcct ttgtcggcgt caaggccgcc gttcttgtga
      181 tcgtcatcga ggcgcttgtc cgcgtcgcaa agcgcgcgct gcgcggacct cacgacgtcg
      241 gcatcgcggc cgccgcgttc gtcgccatct tcttcttcgc cgcgccgttc ccgctcatta
      301 tcgcgacggc cgccgcgatc ggctttctgc ttgcgcgcgc cccgccgcgg ccggccgccg
      361 cgatgccggc gaattccgag gcaatcccgc tctcccgcac gctgaagacc gccggcctct
      421 ggcttgcaat ctggattttg cccctgctcc tgatcgccgc gatcttcggc cgctcgcatg
      481 tcaccacgga tatttcgcta ttcttctcga agcttgcggt cgtcacgttc ggcggcgcct
      541 actcggtgct ggcctatatg gcgcagcagg cggtcgagac ctacggctgg ctcaccgccg
      601 gcgagatgct cgacgggctc ggtttggccg agaccacgcc aggcccgctc attctcgtga
      661 ccgagttcgt gggatttctc gcgggcttcc gccagggcgg cgagcctaag ctcgcgttcg
      721 gcctgctcgg cgcagccgtc acgctgtggg ccaccttcgc gccctgcttc ctctggatct
      781 tcgccggcgc gccctatgtc gagcgcctaa gcgcgaaccc gcggcttgcc ggcgcgctcg
      841 ccggcgtcac cgcggcggtg gtcggcgtga tcctcaattt gagtctctgg ttcgcgctcc
      901 acgtcgtgtt cggcagcgtg accgcggtgt ggcacggtcc ggtgcagctc tggacgccgg
      961 atctcgcgag cctcaatgtc gaggctctca cgctcgcgat tctcgccgcg atccttttgt
     1021 tcggctttca cttcggcatc gtgagcacgc tcgcgatcgc cgccgccgcg gcgcttgcgt
     1081 ggtcgcagct cggattctag cgctcgctaa agttccagag gacgggatgc tgcgcgctag
     1141 cgcggcaccc agagattgac gaagggcgcc ctcacccacg tgccccagcg gccgttatag
     1201 acacgggcaa aaggcgccgc cacccatgtc tcgcggcggg cgtccacatc cgcgaagggc
     1261 gcacgcacgt aagtgccatt gcggcgccag cgtcgtttgt aatagccggc ctcttgcacc
     1321 aaggcggcgc cggcttctgg cgtagccact aggccgggag cggctgaagc gggtgcggcc
     1381 ttgccgacaa ggatcgcaaa gcttgcggcc gcgaaggcga gaattaggga gcgaatgttc
     1441 atgatgaatg tctccttaac caatccggtc ggccgacgcg gctgccgtac ttgccttcaa
     1501 gtatgacggc gaggcaagcc gaaattacag ccagcctctg cgcttgaaat agagataagg
     1561 cacgaccgcc gccagaatca tcagcgcgag cgcgagcgga taaccgaccc accacgacag
     1621 ctccggcatg aattggaaat tcatgccgta gatcgacgcg atcagcgtcg gcggcaggaa
     1681 gacgacggcg gcgaccgaga agatcttgat gatgtcggcc tgctggatgt tgatcatgcc
     1741 cagcgtcgca tcgagcagga agatgatctt gccggagagg aaggtggcat gatcggtcag
     1801 cgaagcgacg tcgcgtgccg cggtgcggat gcgtgcctgg agcggcttga cctctttccg
     1861 ttcgttggcg gcatgaatga ggaaggtcag gagccgcccc aacgagtggg cgctttcacg
     1921 agccttcgag gtaatctcgc cctcgcgtcc gatcgcccgc aacaacacgt cgaatcgcct
     1981 ttgtcgcgag gcggctccgc ctttgatttc gaagatcgag tgggacaggc cctcgacctc
     2041 ggcctgaagg cgctcgatga aatcggcgag gcggtcgatg atcgtctcca acagcccgat
     2101 gagaacggcg ctgccgctct tgagttcggc ctcggaacga ttgcatcgct ccacatagat
     2161 ggagaaagcc cggggctgcg catagcgcac ggtgacaagc cgctggccgg caaggatgaa
     2221 cgtgaccggc gtcgtccggg gctcgccctg atcggagtgg tagagcaggg tcgcggtcat
     2281 gaaataggcg ccgttctcct gatagaggcg gctcgacgcc tcgatttcct gctgctcctc
     2341 gcgcgtcggc acgttgaact tgagcgcccg ttcgaccttc ttctcgtcct cgggcgtggg
     2401 attgaggaga tcgacccaga gcgcttcttc ggtgatccgc ggcttgccct tctgacgctc
     2461 gagcgcgccg gagcggtttt catagatggt gagcatcgga cctcccgttt ccgagcccca
     2521 aggccctcag gccggatgag accttatacc agcacgttga tattgccact gagacaatgc
     2581 ggcccaccaa aggccgaaga tcgcaacaaa gagcctcagt tcttctgcac gatgcaggcg
     2641 cctccttgcg agcgcaggag cttgcagagc ttctcggcat ctgcccggct gtcggcgcca
     2701 atctgcacca tgtagagcga acggcggcct cggctcagat tcctatccgg cagcacgaaa
     2761 ggttcgcggt cgccgaggag cgcctgatgc acccgcttca atctgccgaa ctgggcgagc
     2821 gccttggcct ttgagaatga ggtcgagacg tggactcccc aaggacgcca ggctccgctt
     2881 gccgtcagcg gttcagtctc atacaccgcg cgcaccacga gcggcgcaag cgcgcggcag
     2941 gattccagaa ccgcggcgcc cgcggtcctg agcggctcgg gtaattcggt gtcctcaagc
     3001 ttccactcct ccgccgcgcg tccggtgatg aattgaacgt agttctgcgt ctcccacggg
     3061 agaccgctcg agcctgagag ccacgcccgc acgcgctcgg cgcctgcgtt ataggcggcg
     3121 gccgccagtc cgaaattgcc gaactcccgg tggaggtccg cgaggaagct cgccgaatgg
     3181 acgattgccg atttgggttc gaaaggatct tggagcccgc gctcctcagc cgtgcccggc
     3241 atgaattggg cgatgccttg cgcgcccttc gggctcaccg cttgcgggtt gaagcggctc
     3301 tccttccaga tgagccgcac gaacggcatc ggcggcaggc ctctcgactc tgcctgctgt
     3361 tgcatgatcg gacagacgat atcgacgaag ggctttggtc cggcgagcgc gacgcccgcc
     3421 tccttgaccc ccgtctcgac gctggacgcg gcgttctccg cggcagccga cgatgccgca
     3481 agcagcgaag ccgccagcag aaacgaagcc gcggccgagg ccgcccgcaa ccgtcgcagc
     3541 atgccgtcag tccatccttg ccgggcgact caacttgggg cggagcgtcg cagcaattca
     3601 ccttttgctc aagccgccgg gagccgagca ccagtgcggc gcgggccaca aagaacttcg
     3661 cggactaggc atcgaaggcg cggagcgccg tcgcggcaac gcggtcaagc tccttccgcg
     3721 acgcgccgct tgcggcctgt accgccatgc cctccatgac ggttgcgaga aaccgggcaa
     3781 gcgccggcgg atcggcatcg gccgacaggt ctccctcccg ttttgcccgc ttcaggcgtt
     3841 cgcggatccc ggcttggctc gtcgcgcggc gcttctccag ctcgccgcga atgcagtcgg
     3901 cctcttcacc cgagacaagc gcgccatgca ccgtaaggca tcccttgggg tgacagggat
     3961 cgctcaagaa tttggcccag ccatgcaaaa gcctctcggc gacccggcgc gctgttggct
     4021 gggcgagcgc ctcgtccaac aaggacgtgc gcaaacgcca gtagcggtcc agcgccttgc
     4081 ggaacaggcc ctccttgttg ccgaaggcgg catagaggct cggcggattg atgcccatgg
     4141 cctcggtcag gtcgcagatt gtggcgccct cgtagccttt gcgccagaac acgtcgagcg
     4201 cccggtcgag cgcccggtcg gcatcgaaag cccggggacg gccgatcgcc acggccttgt
     4261 gtcctgatat gcttttgatt tctcacgcct ccttatctgt agcgcttgct atataacccc
     4321 cttgaaatgc gggttcaacg atacatatgt agcatatact acataaccga tgaaatgaaa
     4381 ccgcgtgaag gagcatcgcc gatgatcgaa cggagaccat tcgaggcgct tggcggcgcc
     4441 gaccatggtt ggctcaaggc caagcaccat ttctcgtttg cgcgctacta cgaccccgag
     4501 cgcatggggc acggggcctt gcgcgtgtgg aacgacgacg agatcgctgc caacacgggc
     4561 tttccgcccc acccgcatgc cgacatggag atcatcacct atgtccgcgc gggcgcgatc
     4621 acccatgagg acagccttgg caacaagggc cgcaccgagg ccggcgacgt ccaggtgatg
     4681 agcgcgggct ccggcgttcg ccacgcggag tataaccgcg aatccgtccc cacgacgctg
     4741 ttccagatct ggatcgagac cggcacgcga ggcggcgcgc cgtcctgggg caccaagccc
     4801 tttcccaaga cgggccgctc ggggcgcttc gttgcgctgg cgagcggctt cgcaggcgac
     4861 accgacgcgt tgccaatccg cgccgaggcg cgcgttcttg gcgcgacgct caaatccggc
     4921 gagagcgcgg cgtatgagtt cgcgccaggc cgtcacgcct atctcgttcc ggccaagggc
     4981 aacatcgaag tcaacggtgt ccgcatcgac acgcgcgatg gcgccgcaat caccggcgag
     5041 agcgtgctgg tggtcaaggc cctcgacgac gccgagatcg tcctcgtcga cgcggcatag
     5101 atcgcccgcc gcggtgcgag cgccgcggcg ggttactctt cctcgtcctc gacaacggtc
     5161 tgcggcctgt cggttccagg atcgacctct tcgcgccata cgccgtcggc ggtttcgtat
     5221 tggatgccga cggtctctcc tggaacccgc tgctcgccgg cggcggtcct tgccgcggcg
     5281 agagcttcat cgtggctcgg aaaagtctca gagaacgtgc cgcggacctt ataggcccag
     5341 ccatcctcat gcttgacgat tctgtagacg agcttggcca tggcgctcct cgaaagcgat
     5401 gctattccaa tctcgcacaa ccggcgtcga gcgccaaggt tcctagagcg cgatcaggat
     5461 caccccgatc acggcggcga cgagcccggc gatcttgttc caagtcatcg gttcgccgag
     5521 gaagaggacg ccgagcagcg agccgccgac gatgaacatg ccgtaaatcg gcaccaccac
     5581 attggcgggc ccgagcgaca gcgcatggaa caacgcattc acggcgatcg tgagcgcgat
     5641 gccgccggcg atggcccaaa gcagcggtgg ccgctccagc tcgagcagaa gcggcttgag
     5701 ctcacccctg agcgccacga cggcgatggc gaagatgggg acaatggtgg tggtgacggc
     5761 gagcaccatg tgagtcgagg ccgcgcccga gcgctcggcg agcttcacga acagggtcat
     5821 aagggagtac ccggccatgc cgagcaaggc ccagagcaca taggattgaa ccattcgcgc
     5881 cctccccctc gtagccgtgc gttgctttgc gcacgccgct gcaactctcc cctgctctcc
     5941 ccttgtggga gagcacgcgc cgcttgcggc gcggtgaggg gtcagcggtg aagccgcgtc
     6001 agaaaagttt tacccctcac ccttcccgcc attcgccgcg cttcgcgcgt cgggcgagaa
     6061 gccctctccc tcaaggggag aggggaagat ttcgtgagcc catatcccgc gcggcgatgc
     6121 ctcacccgcg cggcgggcgg cggcgcccgc tgaaccaggc gcgatactcc gtcatcttgt
     6181 cgcgctgcga ttgaggcgcg cgccgcttgc agggcgtcgc ttcctcgctg ccctcggccg
     6241 cgccccatct gagctcgacg cagtcgttga cgttgtatgc catctccagc gccggcatgc
     6301 ggtcgagcgc gtccttgagc gcgagcgtcc attgcgaccc gtcggtgcgc gtataggaga
     6361 acgtgcgagc ggcaagttcc gacgcgagca tgctctccaa ttccgtcttg acctcggcga
     6421 cattcttttc cttgggcatc gcgtagcgat cggggcgccg cgcgacccga tccgggaagc
     6481 cgcgcacgac gtccatggcg atcagcaggc gcaaatcgcg agacggcgtt gcgaaatcct
     6541 cccacgcccc ggtcgtctcg aagatcgacg cgccgtccgg catggccact tcgcggccgc
     6601 cttcgacctg atactttcgt ccgttctcca ccgacgtcac gcgggccttc acctgctcct
     6661 cgagcgccgt gatcgcttcc gtcatcgcgc gcttcggatc gagcggcgcc ggggacatca
     6721 cgtcgtccat gcgatcgtag aagtcttcga cctgaagctt cgattgatcg agagaaaagt
     6781 cgccgtagtc cgcgttattc gcgatctcgt cgttggtcag gcgccgcaag gtcccgttct
     6841 tctcgcgcac gatcgggcgg aagcgcttga accccggacc gccgagcgcg ggatcctgcg
     6901 caaagaggaa gttgccgcgc caataacgct tccgcgcgac ggtcccgtcg ggctgtccgt
     6961 cgattgcgag gaagacgccg gcgccgtcgc ccgattgcgg cacgcgtttg acgaggacaa
     7021 gaatgtgccc gtaaggatcg gcgtagacgg tgccggggcg caacgtctcg gtcgtcagcg
     7081 gcacgggata gtagtcggtg tcgttgtcgt cggccgtcgt tcgcccgttg cccgaatgca
     7141 cggcgtcggc aagcgtcgtc cggagataat atccgaagcc cggcacgagc ccttgcggct
     7201 tccgcggaac ccttgcgact cgcgttacac gcggtggcgg cgctgccggt ccgaacagcc
     7261 cgaacacgcc ggcggacatt ctggccgttt gttcctgctg cacgagcagc gggtccggcg
     7321 gcggttcctc ctttacgatg ttccaccact gctggcaccg cggcggccgg ccgccgtcgc
     7381 cgcgcgtgca cttcgcgtaa ccgaatggca atcccatttt gaaggcgaaa taggcgcgca
     7441 ggaagtaagg cacgtcggcg cagtcgggtt gaatgacgtg ccccttctga tcctcgcgca
     7501 ggccgagatg gtcgaagaga atgttgcgcg acggatcgcg cagcacctcg tgcagcgcct
     7561 tccaggacgg ggcttcctca aggggcgcgt cgaagagctt ctcgatccac gccgaataca
     7621 ggttttcggt cgcccgattc caggtgttgt tgatcggcca aacgctcttg cttgcgcgcg
     7681 ggcgcggcgg ctccttgcgc tgcaccgtga tctcgcgcga aacgtttccg cattccggcg
     7741 cggcaccctc gcgcgcaagc ttcgcctgcc atgtccccgc ttccggcgcc gcgacctcgg
     7801 cgaaccagac gtaaggcggg ccgcccgtgc gagcgcgcga tgcggccgcg accttcccgt
     7861 caggcgcaat cagcgaaagc tcgccttcga gcggcttctc cgcggcgaac accacgcgca
     7921 aggccgcgcc cttccacggc gcgatcggcg aggagagcat cgtaagcccg gcctcgtttt
     7981 cgcaaagctt ggagggatcc gcggcgcggg aggggaagac aatagacgtg caggcgatca
     8041 gcagggctgc gatcgcaaca gcggtcattg agcgttgagg tgacaatcga gctcccccgt
     8101 tcgattccct cggtcgcacc taaaaatacc ccgaaacgag ggccgcaaaa accgcaaatt
     8161 gcacgcccgg cgagcggaaa aagcgggctt aatcccccgc cacatagacc tcgccgccgc
     8221 ttgcgcggaa tttggcgctc atctcggcga tgcccctctc tttctgggcg agcctggcgg
     8281 cagcagcgcg caatgaccgg tgatggcgaa cagaccgtcc ccggccgcgt tgcaggacgt
     8341 actcgccgcg gcggcaattt gacctaaccc ttaacaaatc aagtgattct tgcccccagc
     8401 gcgcatccag tctaagcctc gtcatcattc tgccgtgctt caccttgatt tgcgggcccc
     8461 agggcctcaa ggaggccggc cacccccgcc gcaacaaagg tcgacagggc cgtgaatcaa
     8521 gacgtttcca ccaacacgcc gcagcgcgcc aagggcagag ccgcgaccta tcgttgggtc
     8581 gcgcttctcg tcgtcctggc cgcaaccatc ggcggcgtgc tcctctattg gcgctacgcc
     8641 gagctctatc cctcgaccga caacgcttat accggcgcca atatcgtgcg cgtcgccccc
     8701 caggtctcag gttcggtcgc gaacgtttac gtcagggacg acgataaggt cgccgcgggc
     8761 gatcccctgt tcgatatcga ttccaccctt tacgacgcgg cactccggaa cgcgcgcgcg
     8821 caattcgacg ccgcggcgtc cgccgcaggg accgctgctg acgctctcaa gggcgcggca
     8881 accaagctcg aagagaagcg ggtcgcgctc gaggacgcga taaacaaata ccgcgaagcc
     8941 gagactgcgc agaccgaggg cgaggcgccc tcggcggcgt tcaccgacgc tttgaaggcg
     9001 tggcacgacg cggtaggggc ttacaacgcc gcggcggggg agttcgacgc cgagcaggac
     9061 aagaacctga ccgtcaccac gccgaccgtg cagcttcgcg ccgcttccgc cgcgctcgac
     9121 aaggcaacgc atgagcgcgt gcggacccac gtcaccgcgc ctgcgagcgg ctgggtctca
     9181 aacgtcaatc tcaggcccgg cagcgtcgtc caggccggca cgccagccct tgccttggtc
     9241 gaggacggca attggtgggt cgacgccaac ttcaaggaga cggatctcgg ccgcgtgaaa
     9301 cccgggcaga aggccaggat caaactcgat ctgtatcccg gcgtgatgct cgacggcatc
     9361 gtcgagagca tcagcgcggg atcaggatcg actttctccg tgcttccgcc cgagaacgcc
     9421 acgggcaact gggtcaaggt gacccagcgc ttccccgtca ggatcaggat cacgagcgag
     9481 ccggatcccg acaggccctt gcggctcgga gcgagcgcca cggtgaggat cgacaccacc
     9541 gaacctgctc aaggcggaaa atgagcccag ccaagcgcgt tggcggaaag ggaggcaagc
     9601 gccagccgtc gcccgaaccg cctcgcccga gcctcgccga gctccatccc gagcgcccgc
     9661 cgatcccgct cgtcatcgcc gtggtcctga ccgcggtgct cgaagtgctc gacatcacga
     9721 tcgtcaacgt gtcgatcccc aacatgctcg gcgcctttgc cgcgacgccc gatcagatca
     9781 cctgggttct cacgtcctat atggttgcag ccgccgtggt gatgccgctc accggctatc
     9841 tctccacctg gcttggccgg agaaggctct tgctgctcgc aatcacgggc ttcatcgtct
     9901 cctcggcctt gtgcggcatg gcgtggaacc tcgagagcat ggtgctgttt cgcctggccc
     9961 agggcgtgtt cggcgcgccg ctcgtccccc tgagccaggc gatcctgctc gacgccttcc
    10021 cgcgcgagcg gcacggccag gcactcgcca ttttcggtct cggcatcatg gtcgcgcccg
    10081 tcattggtcc ggcgctcggc ggctacctca ccgagaccta ttcctggcgg atggtgttct
    10141 acatcaacat cccgatcggt ctcgtggcgc tcctgctctc ggtgggctac ttgccgcgcc
    10201 ggcccgtcgc ggaaatcaag accgactggg tgggcatggg acttttggtg ctggccgtgg
    10261 gcgccctgca atatgtgttg gaccagggcc agacccgcga ctggttaaac tccaaagcca
    10321 tcgtcgccgg cagcattatc gccatctttg ccggcgcggc gttcttcatc cgcggctgga
    10381 accgggccgg caacatcatc gacctgtcgc tgctcaagga ccggaacttc gtggcgggaa
    10441 cactcgccat taccgcctac ggcatcagcc tgttcggctg gatggcgatc atgccgctgt
    10501 tcagccagag gcttctcgac tacccaccgg ccaccgcagg caccctgttc attccgcgcg
    10561 gcatcgtcag tgccatcacg ctcgccatca ccggaagtat cctcattcgc ctgttcgacg
    10621 cgcggcatct cgtgatcgcg ggcctggtga tgacagcgct tggaacgctg ccgatggctt
    10681 atttctccct gtatgtggac tattggggca tcgccacgcc gggcatgatc gcgggcattg
    10741 gctcaggcct ctttttcgtg ccgctgaccg cggttgcgtt cgccagcatc ccaagagagc
    10801 agtatgacga ggcggccggc gtctacgcgc tgatgcgggg gatcggcggc tcagccggta
    10861 ttgcgatcgt gagctggctg ttcgtgcggc aggcgcacat tcatttcaac gagctcaccg
    10921 cgcacatcac gccctataac agcgacctcg ttccctatct cggccagcac gggctctcgc
    10981 cctactcgcc cgaggcggcg ccgctcgtcg ttcacgagat tgcgcgccaa gcccagatgc
    11041 tcgccttcaa cgacatcttc tggttcatcg ccatggtgac cttcgccatc attccgctga
    11101 tcttcctgat gaagaggccg gaacgggttt cgctggtgcc ggcttgagcc gcgggcgacg
    11161 gcggcaagag gtcgcgaatg ggatatgccc cgcggggact tgccgcaagg ggcttgcccg
    11221 tcaggcgcga tgcagggccc ttcgaaacta ccagcaggcc ttggcgccgg aggtgcagac
    11281 gtaaggcgag accacgccgg accagcaggt ggtgtaggag tccgacttca cgatatccgg
    11341 cttgtggaaa agttcctggg ccacgcttct gcgccagtaa ggctgaggcg tggcgcgcat
    11401 gggcgatacg gagacggcgc gaattctctt ctgctgcttg taatcggtga tcgcctgagc
    11461 gaccgcggcc tgcgcgcggg tgaccgccgt caccttgtcg aatccgtcag ccgtcccgga
    11521 gaacccgata cacacggcat tggccggtgc gccctgaacc gtgcagagtg ccgcaacaat
    11581 cagtgacagc cccaagacac gcatatacga cctcatcggg aacgcctcct tcggaaagga
    11641 cgaagatgag ctaagattat cccgagcgtg caggcgcacc agtccatttg cggccgcagc
    11701 acgccggtta ccggccgtct gtagcctttg tgcaacagtg gtgacctttt gcgacgaaag
    11761 gaaaaagtcg cgcgatacga gaggcttacg cgcccccaaa tccggcgacc gagaaagggt
    11821 cttcatggcg tcgaagcttg tggtcgttac cggagcgtcg agcgggatcg gagaggcgac
    11881 cgcgacgcgc tacggcgcaa gcggagttga tgttgtcttg cttgcacgga gcgctgaccg
    11941 cctcgacgct gtggcgaaat ctatccgtgc ggcggggggc aacgccacga cctaccccgt
    12001 ggacctggca gacagccgcg ccaccgaaga aacagccgcg cgcatcaagc gcgatttggg
    12061 cacgcctgat attctcgtca acaacgctgg ggccgggccc tggcttccca tggccgagac
    12121 cacggcggag gaagctctcc gcatgatcga ggtgccttat ctcgccggct tcaatctcac
    12181 ccgcaccttt ctccctgcga tgctcgcgcg ccggagcggc gcgatcgcct tcatcacctc
    12241 gcccgcgtcc tacctcgcct ggccgagggc gtcggcctat atcgccgcgc gccgtgccgt
    12301 ggcggggttg gccgagacct tgcagagcga gctcaagggc acgggcgtcg cggtgacgct
    12361 cgtcgtgctc gggacggtgg agacacccta ctggcagcac aaccccggca gccgcgagaa
    12421 catgccgaag accaacccga ggctagtacc ggttctgacg ccggacgaag cggcgagcgc
    12481 catcgtcgat ggcatcgccc agggaagagc gttcgtagtg aaacccggaa tctatcgc
//
LOCUS       DASUQP010000124        10281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_196049,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000124 DASUQP010000000
VERSION     DASUQP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10281
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196049"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VFY74_05570"
     CDS             <1..866
                     /locus_tag="VFY74_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5931204.1"
                     /translation="FEAAISPRTKAIFVESIANPGGIITDLEGVAAVAKEAGVPLIVD
                     NTLATPYLVRPIEHGADIIVHSLTKFLGGHGNSIGGAIVDCGTFDWLASGRYPTLSAP
                     TASYHGITLAETFGNFAFAIACRVLSLRDLGPAISPFNAFLILTGIETLPLRMQRHCD
                     NTLAVATWLSGHDKVAWVNYAGLPGNSFHALAKRYSPKGAGAVFTFGLRGGYEAGIAL
                     VSNVKLFSHLANIGDTRSLIIHPASTTHRQLTDEQRQRAGAGPEVVRLSIGLENVEDL
                     IADLDQALAAA"
     gene            complement(845..1954)
                     /locus_tag="VFY74_05575"
     CDS             complement(845..1954)
                     /locus_tag="VFY74_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX5931205.1"
                     /translation="MSTTAALAGSGSEAAIPARHRRAQIVWLASLAALIVAMILVGGA
                     TRLTDSGLSITEWQPIVGAVPPLSESDWDEAFAAYKTIPEYTELKRGMSLGDFKTIYW
                     WEWTHRFLGRLIAVVFLVPFAVFWIAGFIPPSLLPRLLGLFVLGALQGALGWYMVKSG
                     LTERTDVSQYRLAAHFGLALAILGYTLWLLFGLGGARGKPGAWPARSAAWVAGVMLAL
                     IFVQMLAGALVAGLDAGMGYNTWPLIDGAFVPPGLAAASPWYLNLFENPLTAQFNHRM
                     LGYAVVLVALAQLSWLALKGAPPPLLGSALTLALFALLQAALGVWTLLLFVPVVLGLA
                     HQAGAIAVFGVALYHFWLARSDSFAAVKAQAAASA"
     gene            2393..2596
                     /locus_tag="VFY74_05580"
     CDS             2393..2596
                     /locus_tag="VFY74_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEX5931206.1"
                     /translation="MRGRSRKLFGTIVLLLFLAFYAAIAALIGSGRIAQAPQWVQFAF
                     FLTTGLAWVIPAGLLIRWMQRPD"
     gene            complement(2636..3832)
                     /locus_tag="VFY74_05585"
     CDS             complement(2636..3832)
                     /locus_tag="VFY74_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931207.1"
                     /translation="MTTFGSAGPAAGDAGRSRLERALWRAKAGILALAVGAGLVTLIA
                     LPSRGPLSLLVGLGLAFLAIVYFVARDLIGTPREVETSVGQVRRTSFLPTHRFRPKQE
                     PVAARLQPSAQSAAGFLERFRESLALAESEAEEAARDLGRGGFTGGRRSGNTARENAG
                     LRGGNELRYYLNKRLAAPVSGASRTGIVSKSTADATVLKSRDAVLDHVQAAARSSAPR
                     TILVGGASAMVDAAAEAISIARALVTASDQAVLLDLAHGPRSVSGALGLPRSPGLTDL
                     SAGRAQFEDIIRIDAETPLQLIAAGNPRFAASAEENARLIAIFEALTATYDSVVIHAD
                     RDALRRFAPALRFELSVVVAVLPARNGGAGTTDLGDFSALGCHVIAYEQSGKESRPRF
                     GWAAAV"
     gene            4121..5251
                     /locus_tag="VFY74_05590"
     CDS             4121..5251
                     /locus_tag="VFY74_05590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5931208.1"
                     /translation="MSTLRILHCLRAPAGGLFRHVHDLALGQAQLGAEVGIVCDSLTE
                     GDPVALGRLADNCVLGLTRIPMPRNVGFGDWRVTRKVARLAAKLDVDVLHGHGAKGGA
                     YARLAGRKLKRKGKKVKSIYTPHGGSMHFSPSRPMGRFYLGAERRMLPMSDGLLFESY
                     FAAHRYVELVGQPQCPARIVPNGLHRYEFYDPMLADDAVDFVFVGELKYVKGVDILLE
                     ALAAHQSVFPGRALIVGSGPDEARLRRLARRLGLNSRVAFAGAQSARSAFARARCVVV
                     PSRAESFPYIVLEAAAAQMPLIATDVGGIPEIVGTVDMPLIPSGDVPALAGQLRAFLA
                     TPKIFLERAAALQKIVAERFTVENMTHEIIDFYISEVGAGLS"
     gene            5410..6820
                     /locus_tag="VFY74_05595"
                     /pseudo
     CDS             5410..6820
                     /locus_tag="VFY74_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
     gene            6914..7192
                     /locus_tag="VFY74_05600"
     CDS             6914..7192
                     /locus_tag="VFY74_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931209.1"
                     /translation="MMLSTWIRLVWQSSLLALEAQRVMALRMAALAAGGSRAQAEIQR
                     MFFEKIVASMRVGAMLTMGKPATSIVRHYRSRIRANERRLLARKTRVR"
     gene            complement(7570..8193)
                     /locus_tag="VFY74_05605"
     CDS             complement(7570..8193)
                     /locus_tag="VFY74_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931210.1"
                     /translation="MVDLEAVHGPHHLDFTGAGVGLHRRILYGDQAPPDRLHRLDHAC
                     HGRAVSIFSAYGGNQYGPCYYFEAWPFAVLTVLKVIDPILFGVQRPAAAPWIASGLVA
                     SLMFECAYLPARLEREHRVVVERQDVYRQAEKEGLDNAIVIVASKVGTIRPMPPGDLI
                     RNGLHIGEQKVLYARNRGARNQELLAHFPGREVYFYSNGRLQAATDR"
     gene            complement(8252..9259)
                     /locus_tag="VFY74_05610"
     CDS             complement(8252..9259)
                     /locus_tag="VFY74_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931211.1"
                     /translation="MKERFTDRAEATLGLCRLGLRVTRVDSALTRTAVVLSVGVLLLA
                     MGLHARYQWPRFEEGSEKGTEPRLLVWLVLMAGAVIWLARPGACAMVDVVVAPVRRFG
                     HRAVVAFVVLALAAFLIVANHVLDGFPNSADELAYVMQAQTYAEGRLSIDPPPVYESF
                     SQIHFVALGDKWVSRYAPGWAVILMPAAVLGLPLWTVTPFVGAATLLAFYVLSRRFVS
                     RESAWIGVLTIGLSSFFILNSSSFFNHSLTAFYGVVFAIFGTRYIARGGIWCALAAGL
                     CIGLMGITRTQNAVIFALPYVIALAMTPGRRMGLIWFGLAARPSSRHCSHTITLLLAI
                     P"
BASE COUNT         1966 a         3288 c         3242 g         1785 t
ORIGIN      
        1 gcttcgaagc ggcgatctcg ccgcgcacca aggcgatctt cgtcgagtcg atcgccaatc
       61 ccggcggaat catcaccgat ctcgaaggtg tggccgcggt cgccaaggag gctggcgtgc
      121 cgctcatcgt cgacaatacg ctggcgacgc cctatctcgt gcgccccatc gagcacggcg
      181 ccgacatcat cgtgcactcg ctgaccaagt tcctcggcgg ccacggcaac tcgatcggcg
      241 gcgcgatcgt cgattgcggc acgttcgact ggttggcaag cggccgttat ccgacgcttt
      301 cggctccgac cgcctcctat cacggcatca cgctcgccga gacgttcgga aatttcgcct
      361 ttgcaatcgc ctgtcgcgtc ttgagcctgc gcgatctcgg tccggccatc tcccccttca
      421 acgccttcct catcctcacg gggatcgaga ccttgccgct tcgcatgcag cgccactgcg
      481 acaacacgct tgccgtcgca acctggcttt cgggtcacga caaggtcgcg tgggtgaact
      541 atgcgggtct ccccggcaat tccttccatg cattggcaaa gcgctattcg ccaaaggggg
      601 cgggcgcggt gttcaccttc gggctgagag ggggttacga ggcgggcatc gctcttgtga
      661 gcaacgtcaa gctgttcagc catctcgcca atatcggcga tacgcggagc ctgatcattc
      721 accccgcatc gaccacgcac cgccagctca ccgacgagca acggcagcgc gccggcgccg
      781 gccccgaggt ggtgcggctg tcgatcgggc tcgagaacgt cgaggacctc attgccgatc
      841 tcgatcaggc gcttgccgcg gcctgagcct tcaccgcggc gaacgagtcg ctgcgcgcaa
      901 gccagaagtg atagagcgcg accccgaaga cggcgatggc cccggcctga tgggcaaggc
      961 cgagcacgac ggggacgaaa agaagcaacg tccacacgcc gagcgccgct tgcagcaggg
     1021 cgaagagcgc aagcgtcaac gcgcttccca ggagcggcgg cggagcgccc ttcagcgcaa
     1081 gccatgacaa ttgggcgagc gccacgagca cgaccgcgta gccgagcatg cggtggttga
     1141 actgggcggt gagcggattc tcgaacaggt tgagatacca gggcgaggcc gcggcgaggc
     1201 ccggcggcac gaaggcgccg tcgatgagcg gccaggtgtt gtagcccatg ccggcatcga
     1261 ggcccgccac gagcgctccc gccagcatct gcacgaagat cagtgcgagc atgacacccg
     1321 cgacccacgc tgcgctccgc gcgggccatg cgcccggttt tcctctcgcg ccgccaagtc
     1381 cgaacaggag ccagagcgtg tagccgagga tcgcaagggc aagcccgaaa tgcgcggcga
     1441 gccggtactg gctcacatcg gtacgctccg tgagcccgct cttcaccatg taccagccga
     1501 gggcgccctg gagagcgcca agaacgaaca ggccgaggag ccggggaagg agcgaaggcg
     1561 ggataaagcc cgcgatccag aacacggcaa aaggcacgag gaacacgacg gcgatcagcc
     1621 gcccgaggaa acgatgcgtc cactcccacc aatagatggt tttgaaatcg ccgaggctca
     1681 tgccgcgctt gagctcggta tattccggga tggtcttgta ggcggcgaaa gcctcgtccc
     1741 agtccgactc cgacaaaggc gggacggcgc cgacgatcgg ctgccactcg gtgatggaga
     1801 gacctgaatc ggtgagccgg gtggcgccac caacgaggat catggcgaca atcagcgccg
     1861 caaggctcgc aagccaaaca atctgggcgc ggcgatggcg cgccgggatc gcggcctcgc
     1921 ttccagagcc ggcaagggct gcggtcgtgg acatgaggtc tgatagcggc cggagcgcat
     1981 gaactcaact ccccgccgcc ggcccgtgtg tcgcaacctg cggagttgtt gcaaactttc
     2041 ctttgtgcga acacccaacg ggcgtcaaac ttttgacgaa gaaggcgacc tagatcgaac
     2101 acttcacgag cgaggactag tcgtcccccc accccggcgt cgcaaaggcg acgtcgtccc
     2161 tccccacgag ggggaggggt aaagataagt cacccgacgg catcgagtcg cagtctctcg
     2221 caacctcccc ctggaggtcg gcgcgccgct ctatttgcgc tacctctccg ctgcttgagc
     2281 attcggcgcg ccgggtgggg ggattgtata gtcgacggcc tagcttatga ctcggcgcgt
     2341 aagtcgcgca agcgcaagac ccgtgatgaa ttcagactct ggctgcatct agatgcgagg
     2401 gcgttcgcgg aaactgttcg gcacgatcgt gctgcttctt ttccttgcct tctatgcggc
     2461 aatcgccgcc ctcatcggct cgggccgcat cgcccaggcg ccgcaatggg tgcagttcgc
     2521 cttcttcttg acgacggggc tcgcctgggt gatcccggcg ggacttctca tccgctggat
     2581 gcagcggccg gactgagcgc gcggcgccta ttgacgggcg tgtggctggc ggccgctaga
     2641 ccgctgcggc ccagccgaac cggggacggc tttccttgcc gctctgctca taggcgatga
     2701 catggcagcc aagcgccgaa aaatcgccga ggtcggtggt gccagccccg ccgttgcgcg
     2761 ccggaagcac ggcgacaacg acggaaagtt cgaatctgag cgcaggcgca aaccggcgca
     2821 gcgcgtcgcg gtcggcgtgg atcacgacgc tgtcataggt cgcggtcaac gcctcgaata
     2881 tggcgatcag ccgcgcattc tcctctgctg acgcggcaaa cctcgggttc cccgccgcga
     2941 tcagctggag gggcgtctcg gcatcgatcc tgatgatgtc ctcgaattgg gcccgcccgg
     3001 ccgagaggtc ggtgagaccg ggagagcgcg gcagtccgag cgcgccggaa acggaacgcg
     3061 gcccgtgggc gaggtcgagc agaacggcct ggtcgctcgc ggtgacgagc gcgcgcgcga
     3121 tcgaaatcgc ctctgccgcc gcgtcgacca tggccgaagc gccgcccaca agaatcgtgc
     3181 gcggcgcaga acttcgggcg gcggcttgga catggtcgag cacggcatcg cgggatttca
     3241 gcacggtggc atccgcggtg cttttggaca caatgccggt cctgctcgcg cccgagacgg
     3301 gcgcggcaag tctcttgttc agatagtaac ggagttcatt gccgccgcgc agtccggcgt
     3361 tttcgcgtgc agtgttgccg gaccggcgac cgcccgtgaa tccccctcgc cccaagtctc
     3421 gcgccgcctc ctccgcttcg ctttccgcga gtgcgagact ttcccgaaag cgctcgagga
     3481 agccggcggc cgactgggcg ctcggttgca gcctcgcggc aaccggctct tgctttggcc
     3541 tgaagcgatg cgtcggcaaa aaggatgtcc gtctcacctg ccctacggag gtctcaactt
     3601 cgcgcggcgt gccgatgaga tcgcgcgcaa cgaaatagac gatggcgaga aaggccaagc
     3661 cgagccccac gagcagactg aggggaccgc ggcttggcag ggcgataagg gtcaccagtc
     3721 cggcgccgac ggcaagcgca aggatcccgg ccttggcacg ccaaagggcg cgctcaagcc
     3781 gggagcggcc ggcatcgcct gcggccgggc cagcactgcc gaacgtcgtc aaaagacgga
     3841 actcccacga gacgcgcctt attacagccg cttgctggta accaaatgct taagggccgg
     3901 cccgcatcga aacgggcgca taggctttgt taagggtgca gaccgtccta cggagagtga
     3961 ctgtgccgag ggtgtcacag cgcgcccgtc agctgtgcgc aacacgatac ccatgcccac
     4021 gggcgacatc gatcggcgga gttcggcgct atttaccttg acggatgctt cttctaaagc
     4081 tcacggcgtc ccgtcagagg gtcttggaca cggggtccag ttgagcacgc ttcgaattct
     4141 gcattgtctg cgcgcgcccg cagggggcct gttccggcac gtccacgatc tcgcgctggg
     4201 ccaggctcag cttggggccg aggtcggcat cgtctgcgat tccctgaccg aaggcgatcc
     4261 ggtcgcactc ggccgcctcg ccgacaattg cgtgctcggg ctgacccgta ttcccatgcc
     4321 ccgcaatgtc ggtttcggcg actggcgcgt cacaagaaag gtcgctcgcc tcgccgccaa
     4381 gctcgacgtc gacgtcctgc acggtcacgg cgccaaaggc ggagcctacg cgcggcttgc
     4441 cggccgcaag ctgaagcgga aaggcaagaa ggtgaaatcc atctacacgc cgcatggggg
     4501 cagcatgcac ttctcgccgt cgcggcccat gggccggttt tacctcggag cggaacgcag
     4561 gatgctgccg atgagcgacg gattgctgtt cgagagctac ttcgcggctc atcgctatgt
     4621 cgagctggtc gggcagccgc aatgcccggc gcggatcgtg ccgaacgggc tgcaccgcta
     4681 cgagttctat gatccgatgc tggcggacga cgcggtcgat ttcgtcttcg tcggcgagct
     4741 gaaatacgtg aaaggcgtcg acattctgct cgaagcgctt gccgcccatc agtcggtctt
     4801 tcccggacgc gcgctcatcg tcggcagcgg tcccgacgag gcacggctga ggcggctcgc
     4861 ccgcaggctc ggcctcaaca gccgggtcgc cttcgccggc gcgcagtcgg cgcgcagcgc
     4921 cttcgcgcgt gcgcgttgcg ttgtggtccc gtcgcgcgcg gagtcgtttc cctatatcgt
     4981 gctcgaggcc gcggcggcgc agatgccgtt gatcgccacc gatgtcggcg ggatccccga
     5041 gatcgtcggc accgtcgata tgccgctcat tccgtctggc gacgtgcctg cgcttgcggg
     5101 ccagctgcgc gcttttcttg cgacaccgaa gatatttctc gagcgagccg cggcattgca
     5161 aaagatcgtc gccgagcggt ttaccgtgga aaacatgacg cacgagatca tcgatttcta
     5221 tatctccgaa gtcggggccg gcctctccta gcgggaaaca tcgccgcggg agcgcgatcc
     5281 gcctcgaccg atggccacga ggctaccccc tttgccccgt cggtgcatcc gagcgatggc
     5341 ctaaacgttg cccctgaccc cctgaaatgc caatttgggg tttctacgct cggcggagag
     5401 gccgtctcga tgtccgttcc acagatcacc cccgtgcttc tcgccggcgg ttccggcacg
     5461 aggctctggc cggtctcgcg cgacggcatg ccgaagcagt tcctgccgct cgcaggcgag
     5521 ctttccacct atcagcaggc tctcgcgcgg gtcaaagcgt ccggaggccc gccgatcgtc
     5581 gtcacggcca acgattttcg cttcttcgcc cgccaccagg ccgaggagct tggtatcgag
     5641 gccgacatcg tgctcgaacc ggaagcccgc gattccggtc cggccatcgc cgccggcgtg
     5701 gcgcttgcag ccaagcgcga tccgaacgcc gtcgttctcg cgcttgccgc cgaccatgtc
     5761 attctcgatc aggatcgttt ccaagacgcc tgcgaagagg gacgcgaggt tgcgagcggc
     5821 gactggctcg tgaccttcgg catcaagccg accgagccgc gcacgagcta cggctatatc
     5881 aagcccggcg ttccgctcaa caccaagcat gcgcgcgcgg tcgagcgctt cgtcgagaag
     5941 cccgactccg ctacggccgc gcgctatgtc tccgaaggct atctgtggaa ttcgggcaac
     6001 tttctgtttc gtgccgacgc cttgctcgcc gagctcggcc accatgagcc tgccatggcg
     6061 gaagccacga aagcggcggt ggcgaatgcg gaacgcgatc tcggctttct caggctcgat
     6121 ggcaaagcat tcgccgcggc cccgcggaaa tcgatcgact atgccgtcat ggagaagacc
     6181 gaccgtgccg cggtgatcga agcccgtttc cgctggtccg acatcggaag ttgggacgct
     6241 gttcgacatc ggcgagacgg acaaagccgg caatattctg catgggcctg ccgtcgcgat
     6301 cgattcggaa aattcgctgg tccatgccga tcagcacctg accgcggtcg tgggtgtgaa
     6361 ggatctggtc gtagtgacaa cgcccgatgc ggtgctcgtg ctgccgcggg cgcgcgtcca
     6421 ggaggtgaaa gacctcgtgc gagcgctgaa gagcggcggc cacccggaag cgagaaacca
     6481 ccggcgcaat catcgccctt ggggctccta tgaggcggtc gatgtcggcg accggttcca
     6541 ggtgaagcgc ttgatcgtcc gtccgggcgc ggcgctgtcc ttgcagaagc atatgcaccg
     6601 ggccgagcat tgggtggtcg tgcgcggcac cgccgcggtg atgatcaacg gcgaagagcg
     6661 cctcgtgcat gaaaacgaat cgacctacat cccgctgggc gcggttcatc gccttgccaa
     6721 tcctggcagg atcccgctcg agctgatcga ggtgcagacc ggcagctatc tcggcgagga
     6781 cgacattgtc cgcctcgagg acgtgtacgg ccggggctga gtacggaccg gcggttaggt
     6841 tgaacacgta tcaaagtgct cgacctgacg cggcccaagg ctgtaagtcg ctaaagcctg
     6901 cccgggggaa acgatgatgc tcagcacgtg gatacggctc gtttggcaat cgagtttgct
     6961 cgcactggaa gcccagcgcg tcatggcgct caggatggcc gcgctggccg ctggcggttc
     7021 tcgagcgcag gcagaaatcc agcggatgtt cttcgaaaag atcgttgcgt cgatgcgagt
     7081 cggcgccatg ctgacgatgg gcaaaccggc cacttcaatc gtccgccact accggtctcg
     7141 tatacgggcg aatgagcgcc gcttgttggc aagaaagacc cgcgtgcgct gagctctggc
     7201 acatctcgcg aggatgccat ggaaagttgg tcggagcggg gagatcgagc acccgtatca
     7261 cgccttcaac ctgcgtcgga aaaatgagca tcaccacacg cttcaggcgg tcgaagtcac
     7321 agctgatgga tctctatttt gctgtggccc tgctgacatt tctgttccag ctctgggtcc
     7381 gatcctatca gtgttctgga ttccaggagt gcggtctgag tttcgccaag gcgttcgcct
     7441 ggtctgtcat ctggcccgtt tcgtgggcgg tctacgcggc aggtctgctc tgagagcctg
     7501 tcgtcctttc caccccggta aaagcggaga cgaagccttc cctccactgt gaatgggtcg
     7561 aggcggttgc tagcgatcgg tagccgcctg gagacgcccg ttggaataga aatatacttc
     7621 ccgcccagga aaatgcgcca acagctcttg attccgtgcc ccacgattgc gcgcgtagag
     7681 aaccttctgt tcgccaatgt gaagcccgtt gcgaatgaga tcccccggcg gcatcggccg
     7741 gatcgtaccg actttgctcg ccacgatcac aatcgcgttg tccagtcctt ccttttccgc
     7801 ctgcctgtaa acgtcctgcc gctccacgac gacgcgatgc tcgcgttcaa gtcttgcggg
     7861 taggtaggcg cattcaaaca tcaggctcgc aaccagcccc gacgcaatcc agggtgcggc
     7921 tgccgggcgc tgaacgccga acaggatcgg atcgataact ttaaggacgg ttagaacagc
     7981 gaagggccat gcttcgaaat agtaacacgg tccatactga ttgccgccgt aagcagaaaa
     8041 aatagaaaca gcacgaccgt ggcaggcatg atccaatcgg tgaagtcggt ctggcggcgc
     8101 ttgatcgccg taaagaatgc gacgatgtag cccaacgcca gcaccggtga agtccagatg
     8161 atgaggtccg tgaaccgcct ccaggtcaac caaagtgatt tcgcgctggg ggctccgaac
     8221 ggttcatgcc tggtcgtgac cgctacggca gtcaggggat ggccagtaat aacgtaattg
     8281 tatgcgagca atgccgcgag gaagggcgcg ccgccaagcc aaaccatatc aatcccattc
     8341 ggcgacccgg tgtcatcgcg agtgcgatga cgtacggaag cgcgaagatc acggcgtttt
     8401 gggtgcgcgt gatccccatc agaccaatgc atagtcctgc ggcaagcgcg caccagattc
     8461 ccccgcgcgc gatgtagcgt gtgccaaaaa ttgcgaacac gacaccgtag aaggccgtca
     8521 ggctgtgatt gaaaaaggag cttgaattga gaatgaaaaa agacgagagg ccgattgtaa
     8581 gcacgccaat ccaggcgctt tcccggctga cgaagcgtcg ggagagaacg tagaatgcca
     8641 gcagtgttgc tgcgccgaca aaaggggtca cggtccacag cggcaaacca aggacggcag
     8701 ccggcatcag tatcacggcc catccgggag catatcggct aacccacttg tcgcctaaag
     8761 cgacgaagtg aatttgagag aaggattcat aaacgggcgg cgggtcgatc gagagacggc
     8821 cttcggcata ggtctgagcc tgcatcacat acgcgagttc gtctgcggaa ttggggaatc
     8881 cgtccaacac atggttggca acgatgagaa acgcagccaa ggcaagcaca acgaacgcaa
     8941 ccacggcgcg atgaccaaat cgccgaaccg gtgcgacaac tacgtccacc atcgcgcagg
     9001 cgccgggtcg cgccagccag atcacagcac ccgccatcag aacgagccaa acgagcaacc
     9061 gaggttcggt gcctttttcg ctaccctctt cgaaccgcgg ccattggtag cgcgcgtgca
     9121 gtcccatcgc gaggagcaaa acaccaacgc tcaatacgac tgcggtgcgc gtgagagcag
     9181 aatcaacgcg agtcacgcgc agccccaggc gacataaacc gagtgtcgct tccgcacggt
     9241 ccgtgaacct ctctttcacg actgacctaa acaccccggt gcgagccctt cgagcttgtc
     9301 gtctcgtcta catctccgag gtcggcgcgg gcctgatcct ggcggcaacg cgtcagcact
     9361 ttagctggcg ctgagaaatg gttggagcgg ggagatttga cacccgaccc ccggtcctcc
     9421 agcccgagag gcgcacaaaa atgcgcgtta gcatgacggc ggtgggcgcc cgcttcggcg
     9481 ggcggccacc tcagtcgtcg tcgtcatgtg tcgcttagta acgccagcgc caagggcgcc
     9541 aaggacgcca ataggggcca ccccggtaga caccaccgca gggtctgcac cagcaaccag
     9601 cgggaccaca agcccagaac cgccccgttg ggcatcggcc agggccacca caaccgatgg
     9661 tctccacagg cgagtaattc ggcattggcg gggccgatgg cagcgcctcc gcattcgagc
     9721 cagtgaaagc catcagggct aacaccgtga aaccgattgc cactttatgc atcgacacct
     9781 ccgttgcctt tccgagaaag cgagagagta gcaccgccgc ggctcgtccg cagcccctca
     9841 cctccggcgc ttcagcggcg cctctcggcg ctatcccttc acccggcacg acggagggat
     9901 cgagatttcg aggcccatca tcaacacgct tgccctcgtc aacctcgagg gcgcctacgt
     9961 caccggggag aattctggct ggccgctgga gcgtcagctc tattcgggtc gacgcttttt
    10021 tcgtttgaat tgaacatggt cgcgccaagc acgagcagga tcgcaagtcc agccgcgagc
    10081 accacgccga tcagtacggt attttcggtc ccggttctgc gggtactgcc atctacgggt
    10141 ctatcaggca tagttcgtct ccacgcgccc cgtctagacg catgcacaac cgttcccacc
    10201 tctcggggtt ccaagattag agccatcaaa gcctgtcgga cgacgcggtt gaggccgatc
    10261 gcgccgactc tgcgacggtc a
//
LOCUS       DASUQP010000125        18981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_331570,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000125 DASUQP010000000
VERSION     DASUQP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18981
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331570"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFY74_05615"
     CDS             complement(<1..335)
                     /locus_tag="VFY74_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5931212.1"
                     /translation="MTASGAVLALLALRAVHQGDWKMMFVWLGVALLVDAVDGPLARA
                     LKVETRASRISGERLDLIVDYLTYVAVPAFALTEATLLPEAARLPAAAAMLVSGLFHV
                     ADLTSKTEEG"
     gene            complement(408..746)
                     /locus_tag="VFY74_05620"
     CDS             complement(408..746)
                     /locus_tag="VFY74_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931213.1"
                     /translation="MRSSAVIAGLMALSLGSLPALAFQEMPEPPPADIPAATPQPGAL
                     QLGTPAGETAPATEEGGLNLFGYNVLPKFDFGLDVHYGQDQQQLELQGPDTLDENGDV
                     TVLGKVKRRF"
     gene            complement(910..1110)
                     /locus_tag="VFY74_05625"
     CDS             complement(910..1110)
                     /locus_tag="VFY74_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931214.1"
                     /translation="MRRAETWEVVLNWWLNDYTGLQFQYSQSDISGGPQLFTNAAGVV
                     ASANVNNNAKIEGFGTRLRFDW"
     gene            complement(1065..2237)
                     /locus_tag="VFY74_05630"
     CDS             complement(1065..2237)
                     /locus_tag="VFY74_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5931215.1"
                     /translation="MKVKWKGAPELSSADGKFSFKVRGRLQTDYNAIDQDFDITGRPD
                     VNAAELRRARLGVEGVLFYNVAYKFEVDFANDVTAIKDAYVEYDGLADGLSLRAGNFK
                     TPNSLDEMTSSRFLTFLERAAFVEAWGLDRQIGAGVIYAQDHFTLSAGIFGPEPFDDE
                     IWLKDVKTGAARATFAPINREVNGVHQVIHLGASWRGREGAEDRRSPPSGTSISNPFN
                     DELFRYRARGADLHLADRFVSTPQVFNEDQFWGVEGAVIWGPVHVVGEYTQLEGDIAR
                     ARFPGTPPLLCCNGKDPTYDGWYVEAGWFLTGETRSYKEGEFGRIKVKNPVLGGGKGG
                     GWGAWQIAGRYDVIDLTDQATTIQGNPTSGTFPSSLSANCTQCGEQKPGRLSSIGG"
     gene            2855..4264
                     /locus_tag="VFY74_05635"
     CDS             2855..4264
                     /locus_tag="VFY74_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5931216.1"
                     /translation="MRQGLGAGASAAILLATVSFAVGASPLIKSRSPSESLSSRISPF
                     RQALIYAKEGSPKLVDGRWNLSQSESKLGIKFSADETKQLMACTGQIVCETPGVHDED
                     EDRVRVKVTATAVSVLRPDLLVTAKHVFFKGKRAVVPFGSCSFRSYLRRKVAIPLLVE
                     TDQRKGYVLNNEDFIVVRLKRELEGCSAFAINESDSSLREGEQILAVTGHQVRALNKV
                     SGREPVLAKGKIRRVLDGVLGGPPFYYADIDFDVGGSGGAVFALRDRRPRFPHAGHPQ
                     ATPVLDDEGRLVLRGISVAYGPRAKNNRPYSEERNYTIIIGLQAEFRDLVKGKAHKPA
                     AVELAPCLQAGEAKIDVISESVPLTQPETLAPFLQAYACSREAKPGRKAGKANANCTE
                     FAKGLKRAASQQVKEQHEFRLKNATNCAVCFTYDRCNDYGCWDEAVRLSGDSTLFAGV
                     RVRAPMIKNPQFCKYQFQG"
     gene            4485..5729
                     /locus_tag="VFY74_05640"
     CDS             4485..5729
                     /locus_tag="VFY74_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5931217.1"
                     /translation="MSRALDADRAECPFDVAVVGTGLSGLAAALALAHVGARVAIIGP
                     APAAWRDGTLETRTAALLTSSVDLLKTLGVWARLASEAAPLKAIRMIDASGSPLRAPD
                     ISFKASELGLDAFGHNIANTALVEALYARAQETLPAVIAASVTGVACEAEKAVLSLSE
                     GAPVSGRLVAGADGRHSICRPSARIGVTERHYDQSALATNFRHTLPHRDVSIELHREH
                     GSVTTVPLPDPHASSLIWVGAPAEIAQLTQSSEADFIEALAGRLGDLLGVVSGPGART
                     AFPVTGLTADRLAARRTVLLGEAAHILPPIGAQGLNLGFRDAGALADCVADALARGRD
                     PGGAETLQAYAEARRLDIMTRTLGVDLMNRSLLTSLPPLQAARGILLHGLNALAPLRR
                     AVMRIGLTPPTELPRLMRPGAG"
     gene            5827..6744
                     /locus_tag="VFY74_05645"
     CDS             5827..6744
                     /locus_tag="VFY74_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX5931218.1"
                     /translation="MAQRSTLPLLRDLRYRLEYLVLRFLIGLVRLFPMELAATVSAKA
                     WRLIAPYNRRHKRALLNLERAFPDKTREERERIARAAWENLGRVMVETMNIDRILDDP
                     GRFHVTNGHWIERYKDKMGPVLIVTMHMGNWEIGIWPVTRAGVRPAGVYRQVKNPYVD
                     RYLRAQRQTLYPGGLFGRGRAAGQDETQKTARLIMDYVRQGGRLGFISDLYDANGIAV
                     PFFGYPAKSMPIAAMIARRVGARIWIGRCVRIGNRSCFDVNVNELRIPRTANQAEDIR
                     SITAAIQRHFETWIRETPEQFMWSNRRWS"
     gene            6819..7148
                     /gene="hspQ"
                     /locus_tag="VFY74_05650"
     CDS             6819..7148
                     /gene="hspQ"
                     /locus_tag="VFY74_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEX5931219.1"
                     /translation="MFSRSAKFQIGQVVRHRVYPFRGVIFDVDPTFSNTEEWYRSIPK
                     DVRPDKDQPFYHLLAENADTEYVAYVSEQNLEPDASDEPLRHPHVSELFEDLKNGIFR
                     FRFLQEH"
     gene            complement(7213..8082)
                     /locus_tag="VFY74_05655"
     CDS             complement(7213..8082)
                     /locus_tag="VFY74_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX5931220.1"
                     /translation="MMIGSKYLWNSLRRGAARLALCGGAAALILALAAPVATAQQEGQ
                     VQPKSTQPKSAPPAGAAGGAPAGAPGAAPKAAADAPPQDAWVKLCMKNEQTQNKEICL
                     INHEGLEPNSGMVLITAMVRKVEGEDKQQLIIRLPTAYALVIPAGVQAKIDEGKPIDL
                     QYMICFPAGCQAQTDLTADVFDSMRKGKQMLVAAVNFQQKTMGFPVPLSGFNKAYDGP
                     PADPVKYEESRKQLMEMFKKRQAELAAKAAQAEQKKGTDPTGAMPGAMPPAGQPPAPG
                     MTAPKKPVAPSAQ"
     gene            8382..10163
                     /locus_tag="VFY74_05660"
     CDS             8382..10163
                     /locus_tag="VFY74_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5931221.1"
                     /translation="MSGTAAGAPEAGLAMHGEPREAAGFAHFPYANPKAPKGGRAIFA
                     VPGSFDSVNPLIVKGVAAEGVREYVYESLLARAYDEPFSLYGLIAESVETPDDRSFVE
                     FTLRPEAKFSDGAPVTVADVIFSHAILRDHGRPNHRSYYKKVTKVEQTGPNKVKFTFD
                     SSGDREMPLIIGLMPVLPRHLIDPKLFEKTTLATPVGSGPYKVAKIDPGKGISFKRDP
                     AYWGRDLPVNRGHYNFDEIQFDYYRDAGSMFEAFKSGLVRLRKEEDPTRWTEGYTFPA
                     FRDGRVIKDELPVATPAGMSALVFNTRRPIFADQRVREALIKLFDFEWVNRTLYHGQY
                     ARTESYFARSDLSAHGRPADAAERALLAPFAREVKPAIMDGSFSFPVSDGTGENRDGR
                     QEALQLLEAAGYRLTEGKLINTTTGEPFTFEILAATRAQERLLLTYARALEQVGIEAR
                     IRQIDSAQYQRRKQTYDFDMIQYFWPVSLSPGNEQSFRWGSEGAATEGTLNFPGVKSA
                     GVDAMIEALLKAKDRPSFVAAVRALDRLLLSGDYVIPLFHLPRQWVAHWNDLKRPETT
                     PLYGYQIDTWWIEPERSAADGKEAGKP"
     gene            10171..11781
                     /locus_tag="VFY74_05665"
     CDS             10171..11781
                     /locus_tag="VFY74_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX5931222.1"
                     /translation="MSTTPAETIDGTENGSLPRAELLLRRRAEHKPNVIALSDPPNRP
                     AFGLGNPRSLTYREADVAVDALAAFFAGRDLSPGDVIAVQLPNFAFSPLTLLGAWRAG
                     LSVALLPLLWRGHEIGRASAEIGAKALVGVSCFGDETPIETLSAVAAAQSSIRFVLGF
                     GPHLPDGIGSLDEAINGGREVRQVEAPARTGPAMITFTARAGSPLVTVARSEEELLAQ
                     GAMTVLALDLDGSDVILNPYPLTGPAGLSLGFLPWLISGATLAQHHPFDYNVFAEQLI
                     ATGATVTALPSPVLDELAKDGVPQLPQCRLRRVGAVWTGLEQSGPPAVPFGGEAAFFD
                     LYPLGDLAGIVIKRERAENPAPVPLGEIQIAGGGDDAVFVETKLAPLRDSEGYAEILL
                     RGPVVPDEQAEGPLAPDGDGFIGTGLRGMRSSSTGMDLQLKGDPELLRHGGVAIAASE
                     FDDLYRSFPAFLDAACFVLPDPEAGDRVVAAVVPRPGALVALEALNRFLEERKVAPYK
                     YPDKIVIVRQIPRDADGRVLREQILSQV"
     gene            complement(11787..15311)
                     /gene="mfd"
                     /locus_tag="VFY74_05670"
     CDS             complement(11787..15311)
                     /gene="mfd"
                     /locus_tag="VFY74_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5931223.1"
                     /translation="MASSPRLQHLPVERLLEGPRAVAGGVPEGLDALLLGELARHEAG
                     TGVPILHVARDGTRLATFEEALCFFAPDVEILSFPAWDGVPYDRVAPNAETIARRIAT
                     LAALVGRAPDAQAPLVVLTTVNAIVQRVPPRSFITAASLTLQPGNVIAMQELVERLEI
                     SGYARAGTVTDPGQYAVRGGILDLYPPGREPIRLDFFGDTLEQLRAFDPSTQRTESRR
                     ESLHLLPMSELALTPDVRSSFRQRYVALFGPVTGDDPLYESISAGRQHQGMEHWLPLF
                     HDRLSTLFEYLPGAAVTLDPLVDDARAKRLEQVADHYDARAQALERKAFGAPPYHPVP
                     PETLFLSEAEWQEALSGRHVIVFDPFERPGKPDEAVIVSFGGRQGRSFAAERQTEGAN
                     VFDAVAAHARRLRGEGKRLIVACWSKGARERLATLLSEHGAGETTRVSTFADVLQAPP
                     DSIAVAVLPLEAGFEAPGLAVIGEQDILGDRLVRRTRGTRGSDVLTEASSLTVGDLVV
                     HSDHGIGRFAGLATIEAAGKPHDCLEVHYQGGDKLYLPVENIELLSRYGSEEAGAQLD
                     RLGGVAWQTRKATLKKRIRDIAEKLIKVAALRALHRAPVLTPADGLYNEFSARFPYEE
                     TEDQETSIGAVLDDLSSGRPMDRLICGDVGFGKTEVALRASLIAVLAGKQVAVVVPTT
                     LLARQHFHTFTERFRGFPVKIAHASRLVGARERAEVKKGLKSGDIDIVIGTHALLGKG
                     MEFADLGLLIVDEEQHFGVQHKERLKQLREDVHVLTLTATPIPRTLQLALSGVREMSL
                     ISTPPVDRLAVRTYVMPFDPVILREALLRERFRGGQSFYVVPRIADLDDAAAFLEEHA
                     PELKVACAHGQIGSRELDQVMNAFYDRQYDVLLSTSIVESGLDIPSANTLIVHRADMF
                     GLAQLYQLRGRVGRSKVRAYAYFTIPADARLTPAAEKRLKVLQSLDTLGAGFVLASHD
                     LDIRGAGNLLGEEQSGHIREVGYELYQSMLEEAVTALKGGEGAVIEDQWSPRINLGTA
                     VLIPEDYVPDLQVRLGLYRRLSGVERHEAIEAFAAELIDRFGPLPEEVRHLLDIVEIK
                     GLCREAGIEQIDAGPKGAVIAFRNKSFANPEGLIAFIREEGHRVKLQPDHRLIYYANW
                     QTPEARLAGTRDLLKRLVKIAQAVKQAA"
     gene            complement(15323..15598)
                     /locus_tag="VFY74_05675"
     CDS             complement(15323..15598)
                     /locus_tag="VFY74_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX5931224.1"
                     /translation="MSGNDLDMRRRRALWRATHRGSKEMDVLLGRFAEQAVAGMNAAE
                     IAVFERLIDVPDPAIETCVLEGHSFGEHDLDELIDRVRRFHGLTPAR"
     gene            15712..17856
                     /gene="recG"
                     /locus_tag="VFY74_05680"
     CDS             15712..17856
                     /gene="recG"
                     /locus_tag="VFY74_05680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5931225.1"
                     /translation="MRPAKLAPLFATVRALKGVGPKVEGLLNKLVAPRHEGAHARVVD
                     LVWHLPVGVIDRAIAPRIAEAKIGELATLEVTVTEHRPGGGSKWRSRGPYRVLVEDES
                     GGALQLVYFNADPTYIKRLLPLGSRRLISGKLESYDGWLQMPHPDHVMAIDSEDGAAL
                     PLHEPVYPLTAGLTNTTLRKAIANAIEQVPDLPEWIDTGFLAQTAWPRFGEAVARLHA
                     PETEADLLPSALPRARLAYDELLANQLALAVIRARLKRRAGRRLAAQGKLRKAILAAL
                     PFSLTGAQKRALAEIDADLASPHRMLRLLQGDVGSGKTVVALLAMAAAVEAGLQGALM
                     APTEILARQHLQTISGFASSGFDAAAGLRIALLTGRERGRERAEIMEALKAGRIDILI
                     GTHALFQEQIAFHDLGLVVIDEQHRFGVHQRLALQAKGAGTGAELLVMTATPIPRTLL
                     LTSYGDMDVSRLDEKPPGRKPIATRTLPLERLAEVVSGIARAIAGGAQVYWVCPLVAE
                     SEVLDIAAAEARYAELRARFGESVGLVHGRLAGKDKDRVMAAFASGALSILVSTTVIE
                     VGVDVPKASIMIIEHAERFGLAQLHQLRGRVGRGAQESSCILLYRQPLSETARERLNV
                     MRRTEDGFVIAEEDLRLRGGGELLGTRQSGLPMFRVATLPEHQALLEAARDQARLTLG
                     RDPELKGQGSEALRLLLYLFERDDAIKLMRAG"
     gene            complement(17914..18102)
                     /locus_tag="VFY74_05685"
     CDS             complement(17914..18102)
                     /locus_tag="VFY74_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931226.1"
                     /translation="MTEQPNLIKDAKEWSKQELFSLQNRIEHGRTIAHIAAFLGRTEA
                     EVREKAAALKLKIPDSPI"
     gene            complement(18360..18602)
                     /locus_tag="VFY74_05690"
     CDS             complement(18360..18602)
                     /locus_tag="VFY74_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931227.1"
                     /translation="MASQKTPKSQDQITLVALEQALRDVCQVLKAHDPDRDWDTDAEV
                     QQALAEKLMDLADAGVRDPIELCSRMLEGFGLDAPH"
     gene            complement(18801..>18981)
                     /locus_tag="VFY74_05695"
     CDS             complement(18801..>18981)
                     /locus_tag="VFY74_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931228.1"
                     /translation="AMIIERPFSVEGATRNILNQFAISLKNGPVGMRGIARSYPAART
                     TKFFDVREAPPPAVR"
BASE COUNT         3330 a         6071 c         6027 g         3553 t
ORIGIN      
        1 ccttcttcgg tcttgctggt gagatcggcg acatggaaca agcccgatac cagcatggcg
       61 gcggccgcag gcagacgtgc cgcctcgggc aagagcgtcg cttcggtcag cgcaaaggcc
      121 ggtaccgcca cataggtgag atagtccacg atcagatcga gcctctcgcc gctgatgcgg
      181 gaagcacggg tctcgacctt gagcgcgcgc gcgagcggac cgtcgacggc gtcgaccaag
      241 agagcgacgc ccagccacac gaacatcatc ttccagtcgc cctggtgcac ggcgcgaagc
      301 gcaaggagag cgagcacggc accgcttgcc gtcaagagat gcaccgatgg acctggctca
      361 cagacggggg attggaggtg gctaaggcgg cgttagaaac tatgcgccta gaagcggcgc
      421 ttgaccttgc cgaggacggt gacgtcgcca ttctcgtcca acgtgtccgg accctgcagt
      481 tccaattgct gctggtcttg cccgtagtgc acgtcaaggc cgaaatcgaa cttgggcagc
      541 acattgtagc cgaacaggtt gagcccgccc tcttcggtgg ccggggcagt ctcgccggcg
      601 ggcgtcccga gctggagcgc tccgggttgc ggcgtggcgg ccggaatgtc cgcaggtggc
      661 ggctccggca tctcctgaaa ggcaagcgcg ggcaggctgc cgagcgacag cgccatcaat
      721 ccggctatta cagcagagct acgcatggca gtgatcccac ccgataagat ggctttggta
      781 acggcacaat gcaaggttag ggtgaaccgc cggttaaggc cttgtcgcgg ctcgtaaaaa
      841 acctcgaaaa aaaaccgccg cccttggctc aagggcggtc tggcatgcgg aggggactat
      901 cgatccgctt taccagtcaa aacggagacg cgtgccgaag ccttcgatct tggcgttgtt
      961 gttcacgttg gcggaagcaa cgacgccggc agcgttggtg aacagctgcg ggccgccact
     1021 gatatcggac tggctgtact ggaactgcag gccggtgtag tcgttcagcc accaattgag
     1081 gacaacctcc caggtttctg ctcgccgcac tgagtgcagt tcgcagacaa cgatgaggga
     1141 aaagtcccac tcgtcggatt tccctgtatg gtcgttgcct ggtcggtgag gtcgatgacg
     1201 tcgtagcgtc cggcaatctg ccacgcgccc caaccgccgc ccttgcctcc tccgagcacg
     1261 gggttcttga ccttgatgcg gccgaactcc ccttccttgt aactgcgcgt ctcgccggtg
     1321 aggaaccaac cagcctccac gtaccagcca tcgtacgtgg ggtccttgcc gttgcagcac
     1381 aggagcggcg gcgtcccggg gaagcgcgcg cgggcgatgt cgccctcgag ctgggtatat
     1441 tcgccaacca catgcacagg gccccagatc accgcgccct caacacccca gaactggtcc
     1501 tcgttgaaga cttgcggcgt cgagacgaag cgatcggcga ggtgcagatc ggcgccgcgc
     1561 gcacggtagc ggaacagctc atcattgaac ggattcgaaa tgctcgtccc gctcggcggg
     1621 ctgcgccggt cttcggcacc ctcgcgtcca cgccagctcg cgccgagatg tatgacctgg
     1681 tgcacaccgt tgacctcgcg gttgatcggc gcaaacgtgg cacgcgcggc gccggtcttg
     1741 acatccttga gccaaatctc gtcgtcaaag ggctcgggac cgaaaattcc agccgacaaa
     1801 gtaaagtgat cctgcgcata gatcacgccg gcaccaatct ggcgatcaag accccaggcc
     1861 tcgacgaagg cggcccgctc caagaaagtg aggaaacgcg agcttgtcat ctcatcgagc
     1921 gagttgggcg tcttgaagtt tccggcccgc aagctcaagc cgtcggcgag gccgtcatat
     1981 tcgacatacg cgtccttgat cgcggtgacg tcattggcga agtcgacttc gaacttgtag
     2041 gcaacgttgt agaagaggac gccttccacg ccgagacgcg cacggcggag ttcggccgcg
     2101 ttgacgtccg gtctgccggt gatgtcgaag tcctgatcga tggcgttgta gtcggtctga
     2161 aggcggccgc ggactttgaa cgagaatttg ccgtccgcgc ttgagagttc cggcgcgccc
     2221 ttccacttga ctttcaagtc gagatcgccg ccgccggatt tgccgcggcg gcgtttgccg
     2281 cggccgcttg ggcttgggcg tcggcgacct gcttttgcag cgccctgatc gtggcctgca
     2341 tttcctccat ctgcgcttgg atctgttgca gatcctgcgc tttcgcctcg ggcgcagatg
     2401 ccgataacgc ttgcgcccgc gagcagataa ccgctgagtt tcgttcgtcc ccctgtcatg
     2461 cccgtctcca ctgtcaaaga agttgttccg ctgactcgct cagaacttcg ggaaactctt
     2521 gtcttcttgt gctttggcca ctttttcccg tcgctaccgt tctatttccc gcctaatgac
     2581 gggcttgtga cactcaaacg atggcgcgtt ttttggagag ggctgttgct catcggcaac
     2641 gctggcgcga ccggacgcgg gggcccgcgg gttctgtttg gtgccgaacc ttcaacctga
     2701 agcttacggc ggcctccttg cgcttcctcg acaatggccg ggctttcgtg tctattcgca
     2761 tccacccccc aaaaaggcgg gccgagggaa acggggcttt tcgtcaatgc gaagtcgtag
     2821 ctaattcacg gtcgagcacc tgacgggcag aaccttgaga cagggcctcg gcgctggcgc
     2881 atcggcagcc atattgctgg caaccgtttc attcgcggtg ggagcttcgc cgctcatcaa
     2941 gagccgctcc ccgtccgaat ctctttcttc tcgaatttca ccgttccgtc aggccctcat
     3001 ttacgccaag gaaggaagtc caaagctcgt cgacgggcgc tggaacctca gccagagcga
     3061 atccaagctc gggataaagt tctcagccga cgagacgaaa caactgatgg cgtgcacggg
     3121 gcagatcgtg tgtgagacgc ctggagttca cgacgaggac gaggacagag ttcgtgtgaa
     3181 agttaccgcg accgcggtga gtgtgctcag accggacctg ctggtgacgg cgaagcacgt
     3241 attcttcaaa ggcaaaagag cagtggtgcc ctttggaagc tgtagcttcc gcagctattt
     3301 gcgtcgaaag gtcgctattc cacttctcgt cgagacggac caaaggaagg gatacgttct
     3361 taacaacgag gattttatcg tcgttcgcct caagcgcgaa ttggagggct gcagcgcgtt
     3421 tgcgataaac gagtccgatt catctctgcg ggagggcgaa cagatccttg cagtgaccgg
     3481 tcaccaggtt cgtgccttga ataaagtttc gggccgcgaa ccggtgctcg cgaagggaaa
     3541 gattagaagg gtccttgacg gagtccttgg gggacccccg ttctactacg ccgatattga
     3601 tttcgacgtc ggcggttccg ggggcgcggt tttcgctttg agggatcgac gcccacgatt
     3661 tccgcacgcc ggtcacccgc aagcgactcc ggttttagat gacgaaggtc gcctcgtcct
     3721 gaggggaata tcggtggcct acggaccgcg agcgaagaac aacaggccgt acagcgaaga
     3781 gcgaaactat acgatcatca ttggccttca ggcggaattt agagatctgg tcaagggcaa
     3841 agctcataag ccggctgctg tggaactggc gccctgtctc caagctgggg aggctaagat
     3901 cgatgtgatt tcagaatctg ttccgttgac tcagccggaa acgcttgcac cttttctcca
     3961 ggcatacgcg tgcagccgcg aagcgaagcc cggccgcaaa gctggaaagg cgaacgccaa
     4021 ttgcacggag ttcgcaaagg gattgaaacg agccgcatcg caacaagtaa aagaacaaca
     4081 tgaattcagg ctgaagaacg ccacgaattg tgcggtctgc ttcacctatg atcgttgcaa
     4141 cgattacgga tgttgggacg aagcggtgcg gttaagcggc gactcaacac ttttcgccgg
     4201 cgtgagagtc cgggcaccca tgatcaagaa cccgcaattc tgcaagtatc agtttcaggg
     4261 ctgaacacgc attggacacc catcgccacg ctcgagcgtt gcctcgcggc gccacgaccc
     4321 gctcttttga cctgtgtggt gctcatcggc aacggctccg cggcgcgcac ggtcctcaag
     4381 tcgcgccggc aatctccgcc tttgtcttgt ttgtgaatgc aatagaatgg caccgcgatc
     4441 ggcgccctgc cccggggcga ccgaccaagg cctatgagcg gagaatgagc cgagcgctgg
     4501 atgcggacag ggccgaatgc cctttcgacg ttgccgtggt cgggacgggc ctttcggggc
     4561 ttgctgctgc gcttgcgctc gcccatgtcg gcgcccgcgt cgcgatcatc ggtccggcgc
     4621 cggcggcctg gcgtgacggt acgctcgaga cgaggaccgc cgcacttctc acaagctccg
     4681 tcgatctgtt gaagacgctc ggcgtctggg cgcggctggc aagcgaggcg gcaccgctca
     4741 aagcgatccg gatgatcgat gcgtcgggca gccctttgcg cgctccggac atcagtttca
     4801 aggcgagcga gctcgggctc gacgccttcg gccacaacat cgccaacacc gcgctggtcg
     4861 aggcgctcta cgcgcgcgcg caagaaactt tgccggccgt cattgccgcg agcgtcaccg
     4921 gtgttgcttg cgaggcggag aaggcggtct tgagcttgag cgagggtgca cccgtctcgg
     4981 ggcgtctcgt tgcgggagcc gacggtaggc actcgatctg ccgcccgagc gcacgaatcg
     5041 gcgtgacgga gcggcattac gatcaatcgg cactcgccac gaattttcgc cacacccttc
     5101 cccatcgaga cgtctcgatc gagctgcaca gggagcacgg gtcggtcact acggttcccc
     5161 tgcccgatcc gcatgcctcg agcctgattt gggtcggggc gcccgcggag atcgcccaac
     5221 tgacgcaatc gagcgaggcg gatttcatcg aagcgctcgc cggacgcctt ggcgatcttc
     5281 tcggcgtggt gtccgggccc ggcgcacgga ccgcattccc ggtgacgggg cttacggccg
     5341 accggcttgc ggcgagacgc accgtgctcc tcggcgaagc cgcgcatatc ctgccgccga
     5401 tcggcgctca gggtctcaat ctcggcttcc gggacgccgg cgcgcttgcc gattgcgtcg
     5461 ccgatgcgct ggcgcgaggg cgtgacccag gcggcgcgga gacgcttcag gcctatgccg
     5521 aggcccgccg gctcgacatc atgacccgaa cgctcggggt cgatctcatg aaccgttcgc
     5581 tgctgacctc gctcccgcct ctgcaagcgg cgcgcggcat ccttctgcac ggactgaatg
     5641 ctcttgcgcc gctgcgccgc gccgtcatgc ggatcgggct caccccgccg acggagctgc
     5701 cccgtctcat gcgcccgggg gctggctgag ttgacgcaag tcttgacgct ccacgccgta
     5761 tttgccacga gaaagccttc accccgcttg catccttcca actgaaacaa cacgctcgcg
     5821 ccgcccgtgg cccaacgctc aacactcccg ctcttgcgcg acttgcgcta ccggctcgaa
     5881 tatttggttc tgcgctttct gatcggtctc gtccggctgt tccccatgga actcgcggca
     5941 accgtctcgg cgaaggcttg gcgtctcatc gccccctaca atcgccgtca caagcgcgcg
     6001 cttctcaatc tcgaacgtgc atttccggac aagacgcggg aggagcggga acgcatcgcc
     6061 agagccgcat gggagaatct cggtcgcgtc atggtcgaga ccatgaatat cgaccgcatt
     6121 ctcgacgatc cggggcgctt ccacgtaacc aacgggcatt ggatcgagcg ctacaaggac
     6181 aagatgggac cggtcctgat cgtgaccatg cacatgggca attgggagat cggcatatgg
     6241 ccggtgacgc gcgcgggcgt gcgcccggcc ggcgtttacc ggcaagtgaa gaacccatac
     6301 gtcgaccgct atctccgtgc ccagcgccag acgctttatc cgggagggct gttcggacgc
     6361 ggccgggccg cgggccagga cgagacgcag aagaccgccc gcctgatcat ggactacgtg
     6421 cgtcagggag gacggctcgg cttcatctcc gatctctacg acgcgaacgg gatcgcggtg
     6481 cccttcttcg gctatcccgc caagagcatg ccaattgcgg ccatgatcgc gcgccgggtc
     6541 ggggcccgca tctggatcgg ccgctgtgtc aggatcggca accgcagctg cttcgacgtg
     6601 aatgtcaacg agctcaggat cccgcgcacg gcaaatcagg cggaggatat ccgctccatc
     6661 accgccgcca tccagcgcca tttcgagacc tggatcagag agacgccgga gcaattcatg
     6721 tggtcaaacc ggcgctggtc ctaggtcccg cccccacggc cgttgacagc accgtcaccc
     6781 ttggctctta tcgtcttgag gactcacagg ttgccgtcat gttttcccgg agcgccaaat
     6841 tccagatcgg ccaggttgtc cgccatcggg tctacccgtt tcgtggcgtg atcttcgatg
     6901 tggacccaac cttcagcaac accgaggagt ggtaccgttc catccccaag gacgtgaggc
     6961 cggacaagga tcagcccttc taccatctgc tcgccgaaaa tgccgacacc gagtacgtcg
     7021 cctacgtgtc ggagcagaac ctcgagccgg acgcttccga cgagccgctg cggcatcccc
     7081 atgtgtcgga actgttcgag gacttgaaga acggcatctt tcgcttccgc tttctgcaag
     7141 agcactagcg cggccaataa agcgaaaggc cgccccggtg ggcggccttt gaatagtgca
     7201 ggagtcgcgc gtttattgtg cagacggcgc aaccggcttc ttgggcgccg tcattccggg
     7261 agcaggaggc tgaccggccg ggggcattgc ccccggcatc gcgcccgtcg ggtccgttcc
     7321 cttcttctgc tccgcttggg ccgcctttgc cgcgagttca gcctggcgct ttttgaacat
     7381 ctccatcagc tgcttgcgcg actcctcgta tttcacgggg tcggccggcg ggccgtcata
     7441 agccttgttg aatccgctca aaggaaccgg aaagcccatc gtcttctgct ggaagttcac
     7501 ggccgcaacg agcatctgct tgcccttgcg catgctgtcg aacacgtccg ccgtcagatc
     7561 ggtctgggcc tgacagcctg cgggaaagca gatcatgtat tgcagatcga tcggcttgcc
     7621 ctcgtcgatc ttggcctgca caccggcggg gatcacgagc gcgtaggcgg tcggcaagcg
     7681 aatgatgagc tgctgcttgt cctcgccttc aaccttgcgc accattgcgg tgatcaacac
     7741 catgccggaa ttgggctcga gcccttcgtg attgatgagg cagatttcct tgttttgggt
     7801 ctgctcgttc ttcatgcaaa gcttgaccca ggcgtcctgg ggcggagcgt cggcagccgc
     7861 cttcggcgcg gcgcccggag caccggcggg cgccccgcct gccgctcccg ctgggggtgc
     7921 tgatttgggc tgcgttgatt tgggctgaac ctgaccctct tgttgggctg tcgccacagg
     7981 cgccgcgagc gccaaaatga gtgccgccgc gcctccgcac agcgccaatc gagccgcgcc
     8041 gcggcgcagc gaattccaaa ggtatttcga cccgatcatc atcgaccctt cctcttttcg
     8101 cgggctggtt caggtcttcg cgatgaccct tgttcgccca ccgtcccgtc gtagttggtc
     8161 aatacctgaa atgaggcata ggcgtggctg agaccgcctc tcgaggcgat cgcagtcaac
     8221 ccgtatgggg ccgcaacccc cgaccgccgc actgacagcg ccaattgcgt tccgggcgat
     8281 gttaccctta actccgtgct gcaatgcgaa atgcatctat agcccacagg cgcgcgctct
     8341 tgaaggcccc aatttcattg ctctttttga gcctcgtcct gatgtccggc actgccgcgg
     8401 gcgcaccgga ggccggactt gccatgcatg gcgaaccgcg cgaggcggcc ggatttgccc
     8461 attttcccta tgccaatccc aaggctccca aaggcgggcg cgcgatcttc gcggtgccgg
     8521 gctccttcga cagcgtcaat cccctcatcg tcaaaggcgt ggcagccgag ggtgtgcgcg
     8581 aatacgtcta tgagagcctg ctggcccggg cctatgacga gcctttcagc ctttacggcc
     8641 tcatcgccga gagcgtggaa acgcccgacg accggagctt cgtcgagttc accctgcgcc
     8701 cggaggcgaa gttctccgac ggcgcccccg tcaccgtcgc cgatgtgatt ttctcgcacg
     8761 ccatcttgcg cgatcacggt aggcccaatc accgctccta ctacaagaag gtgaccaagg
     8821 tggagcagac cggaccgaac aaggtgaaat tcaccttcga ctcgagcggc gatcgcgaga
     8881 tgccgctgat catcggattg atgccggtcc tgccgcgcca tctcatcgat cccaagctgt
     8941 tcgagaagac cacgctcgcc actcccgtcg gcagcggccc ctacaaggtg gccaagatcg
     9001 atcccggcaa gggcatctcc ttcaagcgcg atcccgccta ttggggccgc gatctgccgg
     9061 tcaatcgcgg gcactacaat ttcgatgaga tccagttcga ttactaccgc gacgccggca
     9121 gcatgttcga ggccttcaag tcggggctcg tccgtctgcg caaggaagaa gatccgaccc
     9181 gttggaccga gggctacacc tttcccgcct ttcgcgatgg gcgcgtcatc aaggatgaac
     9241 tgccggttgc aactccggcc ggcatgtcgg cgctcgtctt caacacgcgg cggccgatct
     9301 tcgccgatca gcgcgtgcgc gaggcgctga tcaaactgtt cgacttcgaa tgggtcaacc
     9361 gcacgctcta tcacggtcaa tatgcacgca cggagagcta ctttgcccgc tcggacttga
     9421 gcgcgcacgg gcgccccgcc gatgccgccg aacgggccct gcttgcgcct ttcgcgcgtg
     9481 aggtgaaacc ggccatcatg gatggaagct tctcctttcc ggtcagcgac gggaccggcg
     9541 agaaccgcga cggccggcag gaagcgcttc agcttttgga agcggccggc tatcggttga
     9601 ccgaaggcaa gctcatcaac acgacgacgg gagaaccgtt cacgttcgag atcctcgcgg
     9661 ccacgcgcgc ccaggagcgg ctcctgctga cttatgcgcg cgcccttgag caggtgggca
     9721 tagaggcacg aatccgccag attgattccg ctcaatacca gcggcgaaag cagacgtacg
     9781 acttcgacat gatccagtat ttctggccgg tgtccctgtc gcctggaaat gagcagtcgt
     9841 tccggtgggg gagcgagggc gcggcgacgg aaggcacgct caactttccc ggggtgaaga
     9901 gcgcgggcgt cgacgccatg atcgaggcgc tgctcaaggc caaggaccgc ccgagcttcg
     9961 tcgccgcggt gcgcgctctc gatcgcctcc tgctctccgg cgactatgtc attccgttgt
    10021 tccatctgcc gcgccagtgg gtcgcgcatt ggaacgattt gaagcggccg gagacgaccc
    10081 cgctctacgg ctatcagatc gacacgtggt ggatcgagcc tgagcgcagt gcggccgacg
    10141 gcaaagaggc ggggaaaccg tgaccggacc atgagcacaa cccctgctga aacgatcgac
    10201 gggaccgaga acggaagcct gcccagggcc gaactgctgc tccggcgccg ggccgagcac
    10261 aagccgaacg tcatcgcgct ttccgatccg cccaatcgtc cggcctttgg tctcggcaac
    10321 ccgcggtcct tgacctatcg cgaggccgac gtggcggtcg acgcgctcgc cgccttcttc
    10381 gccggtcgtg atctcagccc cggcgatgtc attgccgtgc agctgccgaa tttcgcgttc
    10441 tcgccgctga cgctcctggg tgcttggcgc gccgggctct ccgtcgcgct ccttccgctg
    10501 ctttggcggg gacacgagat cggcagagct tccgccgaaa tcggggccaa ggcgcttgtc
    10561 ggtgtctcct gtttcgggga cgagacgccg atcgagacac tctccgcggt ggccgccgct
    10621 cagtcatcga tccgcttcgt gctcggtttc ggtcctcatc ttccagatgg catcggctcc
    10681 ctcgatgagg cgataaacgg aggccgggag gtgaggcagg tcgaggcccc ggcacggacg
    10741 ggaccggcta tgatcacatt tacggcccgc gcaggttcgc ctctcgttac ggtcgcgcgc
    10801 agcgaggagg agcttctggc gcaaggggcg atgacggttc tcgcgctcga cctcgacggg
    10861 agcgacgtca tcctcaatcc ctatcccctg accggaccgg ccgggctttc cttggggttt
    10921 ctgccgtggc tgatcagcgg tgcgaccctc gcccagcatc atcccttcga ttacaacgtc
    10981 ttcgccgagc agctgatcgc cacaggggcg acggtgacgg cgctcccctc gcctgtcctt
    11041 gacgaactcg ccaaagacgg cgtgccgcag ctgccccagt gccgcttgcg gcgtgttgga
    11101 gctgtctgga ccgggctcga gcaatcaggc ccgccggcgg tcccctttgg gggcgaagcg
    11161 gcgttcttcg atctctatcc gctcggcgat ctcgccggca tcgtgattaa gcgcgagaga
    11221 gcagaaaatc ccgctccggt tcctttgggc gagatccaaa tcgccggtgg cggcgacgac
    11281 gcggtcttcg tcgaaacgaa gctcgccccg ctccgcgact cggaaggcta cgccgagatc
    11341 cttctgcgcg gaccggtcgt tcccgacgaa caggcggagg ggccgcttgc ccccgacggc
    11401 gacggcttca tcggcaccgg gctgcgcggg atgcgcagca gctcgacagg catggatctg
    11461 cagctcaagg gcgatcccga gcttctccgt cacggcggcg tcgcgattgc ggcttccgag
    11521 ttcgatgatc tctaccggag cttccccgct ttcctcgacg ccgcatgttt cgtgctcccc
    11581 gatcctgaag ccggagaccg cgtcgttgcc gccgtggtgc cgcgacccgg cgcgcttgtc
    11641 gcgctggagg cgctcaatcg gtttctcgag gagcggaagg tcgctcccta caaatacccc
    11701 gacaagattg tcatcgttcg gcagatcccg cgcgacgccg acggccgcgt gctgcgcgag
    11761 cagatcctgt cgcaggtcta ggcgggtcag gccgcctgct tcaccgcctg ggcgatcttg
    11821 acgagccgct tcaacagatc gcgcgttccg gcgagccgcg cctccggcgt ctgccaattg
    11881 gcatagtaga ttagccggtg atcgggctgc agcttcaccc tgtggccctc ctcgcggatg
    11941 aaggcgatca ggccttcagg attggcgaag ctcttgttgc ggaaggcgat caccgctccc
    12001 tttggcccag cgtcgatctg ctcgatgccg gcctcgcggc acagtccctt gatctcgacg
    12061 atatcgagga ggtggcgcac ctcttcgggg agcgggccga agcggtcgat cagctcggcg
    12121 gcaaaggcct cgatcgcctc gtggcgctcc acacctgaga gcctgcgata gagcccgagc
    12181 ctcacttgca gatcgggcac gtagtcctcg gggatgagca ccgcggtgcc gagattgatg
    12241 cgcggcgacc attgatcctc gatcacggcc ccctcgccgc ccttgagcgc ggtcacggcc
    12301 tcctccagca tcgactggta gagctcgtag cccacttccc ggatatgtcc cgactgttcc
    12361 tcgccgagaa gattgcccgc gccccggata tcgagatcgt ggctcgcaag gacgaagccg
    12421 gcgccgagcg tgtccagcga ttgcagcacc ttgaggcgct tctcggcggc gggcgtgagc
    12481 ctcgcatcgg cgggtatggt gaaataggcg taagccctga ccttggaacg acccacccgt
    12541 cctcgcagct gatagagctg cgccagcccg aacatatcgg cgcggtgcac gatcagcgtg
    12601 ttggccgaag gaatgtcgag gcccgattcc acgatcgagg tggacaagag cacgtcgtat
    12661 tggcggtcgt agaaggcatt catcacctgg tcgagctcgc gcgatccgat ctgtccgtgg
    12721 gcgcacgcca ccttcagctc cggagcatgt tcttcaagga aagcggcggc gtcatcgaga
    12781 tcggcaatgc gcggcacgac atagaagctc tggccgccgc ggaagcgctc gcgcaagagc
    12841 gcctcgcgca gaatgacggg atcgaacggc atgacataag tccgcacggc gagcctgtcg
    12901 accggaggcg tcgagatcag cgacatctcg cgcacgccgg acagcgcaag ctggagcgtg
    12961 cgggggatgg gcgtggcggt cagcgtcagc acatgcacgt cctcgcggag ttgcttgagg
    13021 cgttccttgt gctgcacgcc gaaatgctgc tcctcatcga cgatcaggag cccgagatcg
    13081 gcgaactcca tccccttgcc gagcagtgca tgggtgccga tgacgatgtc gatatcgccc
    13141 gatttcaagc ccttcttcac ctcggcgcgc tcccttgcgc cgaccaggcg tgaagcgtgg
    13201 gcgatcttca cgggaaagcc gcggaagcgc tcggtgaagg tatggaagtg ctggcgagcg
    13261 agcagcgtgg tcggcaccac gaccgcgacc tgcttgccgg caagcacggc gatgaggctc
    13321 gcgcggagag cgacttccgt cttgccgaac ccgacatcgc cgcagatcag tcggtccatg
    13381 ggacgcccgc tcgaaagatc gtcgagcacc gcgccgatgc tggtctcctg gtcctccgtc
    13441 tcttcgtatg ggaaccgcgc cgaaaactcg ttatagaggc cgtcggccgg ggtcaggacc
    13501 ggggcgcgat gcaaagcgcg cagcgctgcg accttgatca gcttttccgc aatgtcgcgg
    13561 atgcgcttct tgagggtcgc tttgcgtgtc tgccaggcga cgccgccgag cctgtcgagc
    13621 tgggcgccgg cctcctcgga gccgtagcgg ctgagaagct cgatattctc gaccggcaga
    13681 tagagcttgt cgccgccttg gtaatgcact tcgagacaat cgtgcggctt gccggcggcc
    13741 tcgatggtgg ctaaccctgc gaagcggccg atgccgtgat cggaatggac cacgagatcg
    13801 ccgacggtca agctcgacgc ttcggtcagg acgtcgctgc cgcgggttcc tcgcgtgcgc
    13861 cgcaccagcc tgtcccccaa gatgtcctgt tcgccgatca cggcaagccc gggcgcttcg
    13921 aagccagctt ccagcggcag caccgccacc gcaatggaat cgggcggcgc ctgaagcacg
    13981 tcggcgaacg tgctgacgcg cgtcgtctcg ccggcgccgt gctccgacag cagcgttgcc
    14041 agccgctcgc gcgcgccctt gctccaacag gcgacgatca agcgctttcc ctcgcccctc
    14101 aacctcctgg catgagcggc cacggcgtcg aagacgttgg cgccctcggt ctggcgctcg
    14161 gcggcgaaag agcgtccctg cctcccgcca aaggagacga tgaccgcttc atcaggcttc
    14221 ccagggcgct cgaacggatc gaagacgata acgtggcgcc ccgagagcgc ctcctgccat
    14281 tcggcctcgc tcaggaacag ggtctccggc ggcaccgggt ggtaaggcgg cgcgccgaag
    14341 gccttgcgct ccagcgcctg cgcgcgcgcg tcgtagtggt cggcaacctg ctccaggcgc
    14401 tttgcgcgcg cgtcgtcgac aagcggatcg agcgtgacgg cggcgcccgg cagatattcg
    14461 aacagcgtgg aaaggcggtc atggaagagc gggagccaat gctccatgcc ctggtgctgg
    14521 cgcccggcag agatcgactc gtaaagcgga tcgtcgccgg tgacgggacc gaacagcgcg
    14581 acataacgct ggcggaagga gctgcgcacg tcgggcgtca gcgccagctc gctcatcgga
    14641 agaagatgga gactttcgcg ccggctctcg gtccgctgcg tacttggatc gaaggcgcgc
    14701 agctgctcga gcgtatcgcc gaagaaatcg aggcggatcg gctcgcggcc cggaggatag
    14761 agatcgagaa tgccgccgcg caccgcatat tgcccggggt cggtcacggt gccggcgcgc
    14821 gcatagccgg agatctccag gcgctcgacc agctcttgca tggcgatgac gttgccgggc
    14881 tgaagcgtga ggctcgcggc cgtgatgaag ctccgtggcg gcaccctctg cacgatggca
    14941 ttgacggtcg tcagaacgac gaggggtgcc tgcgcgtcgg gcgctcgacc gacgagcgca
    15001 gcaagcgtgg cgatccgccg cgcgatagtt tccgcgtttg gcgcgacccg gtcgtaaggg
    15061 acgccgtccc aggcagggaa gctcaaaatc tcgacgtcag gcgcaaagaa gcagagcgcc
    15121 tcttcgaagg tggcaagccg cgtgccgtcg cgcgcgacat gcaggatcgg gaccccggtc
    15181 cccgcttcgt ggcgggcgag ttccccgaga agaagcgcat cgagcccctc cggcacgcct
    15241 ccggcgacgg cgcgcggccc ctccaacagg cgctcgacgg gcaaatgctg aagtctaggc
    15301 gaagatgcca tggcttggtc ggtcagcgcg ccggggtcag accgtggaaa cgtctcacgc
    15361 ggtcgatcag ttcgtcgagg tcgtgttcgc cgaaggaatg tccttcgagc acgcaagtct
    15421 cgatagcggg gtcgggcacg tcgatcaggc gctcgaacac ggcgatttcg gcggcattca
    15481 ttccagcaac ggcttgctcg gcaaagcggc cgagcaggac atccatctcc ttcgagccgc
    15541 gatgcgtcgc ccgccacagc gcccggcggc ggcgcatgtc aagatcattg ccggacatga
    15601 taatgctttc ctgagccgca aggggtgggt tagagcgagc gcgccgagcc tgtcaaggtc
    15661 gcgcgccctc gtcaaagccg tcacgccaaa tgcacgtttc cctagaagac catgcgaccc
    15721 gctaagttgg cgcccctctt cgctaccgtg cgcgccttga agggcgtggg gccgaaggtc
    15781 gaagggttgc tgaacaagct cgtggcgccg cgccatgagg gggcgcatgc gcgcgtcgtc
    15841 gacctcgttt ggcatcttcc cgtcggcgtc atcgaccgcg cgattgcacc gcgcattgcc
    15901 gaggccaaga tcggcgaact cgcgacgctc gaagtcacag tgaccgagca caggccgggc
    15961 ggtggaagca agtggagatc gcgcgggccc taccgcgtgc tcgtcgagga tgaatcgggt
    16021 ggcgcgctcc agctcgtcta tttcaacgcc gatccgactt acatcaagcg cctcctgccg
    16081 ctcggcagcc gacgcctgat ctccggaaaa ctagagtctt atgacgggtg gctccagatg
    16141 ccgcatcccg atcatgtgat ggcgatcgat tctgaggatg gcgcggcgct tccgctgcac
    16201 gagccggtct atccgttgac tgcggggctc accaacacga cgctgcgcaa ggcgatcgca
    16261 aacgcgatcg aacaggtgcc tgatttgccg gaatggatcg acaccggctt tctcgctcag
    16321 actgcctggc ctcgtttcgg cgaggcggtt gcgcgtctcc atgccccgga gaccgaggcc
    16381 gatcttctgc cttcggcgct cccacgggcc cggctcgcct acgacgagct tctcgccaac
    16441 cagcttgcgc ttgccgtgat ccgcgcgcgg ctcaagcggc gtgccggacg gcgtcttgca
    16501 gcgcagggga agttgcgcaa ggcgatccta gccgcgctcc cattttctct cacgggggcg
    16561 cagaagcgcg ccttggccga gatcgacgcc gatcttgcga gcccccaccg catgcttcgc
    16621 ctgctccagg gcgatgtcgg cagcggcaag accgtcgtcg ccctgctcgc catggcggcg
    16681 gccgtcgagg cgggcttgca aggcgcactg atggcgccga ccgagattct ggcgcgacag
    16741 catctgcaaa cgatcagcgg attcgcctct agcggctttg acgccgctgc gggcctgcgg
    16801 attgcgcttc tcaccggccg cgagcgcgga cgcgagcgcg cggagatcat ggaggctctc
    16861 aaggcggggc gcatcgatat cctgatcggc acccatgcgc tgttccagga gcagattgcg
    16921 tttcacgatc tcggcctggt cgtcatcgac gagcagcatc gattcggcgt gcatcagcgg
    16981 cttgcgctgc aggccaaggg cgcagggaca ggcgccgagc ttctggtgat gacggctacg
    17041 ccgatcccgc gcacgcttct gcttacgagc tatggcgaca tggatgtgtc gcggctcgac
    17101 gaaaagcctc cggggcggaa gccgatcgcg acccgcaccc tgccgctcga acggctcgcc
    17161 gaagtcgtct cgggaatcgc gcgggccatc gcgggcggcg cccaggttta ttgggtgtgc
    17221 cccctcgtcg ctgagtcgga agtgctcgat atcgccgcgg ccgaggcccg ctacgccgag
    17281 ctccgcgcca ggttcggcga gtcggtcggg ctggtccatg ggcggcttgc cggcaaggac
    17341 aaggaccgcg tcatggcggc gttcgcttct ggggcgctgt ccatcctcgt ttccaccacc
    17401 gtgatcgagg tcggcgtcga cgtgccgaag gcctcgatca tgatcatcga acatgccgag
    17461 cgctttgggc ttgcccagct gcatcagctg cgtggcaggg tcgggcgcgg cgcgcaagag
    17521 tcgtcgtgca ttttgcttta ccgccagccc ctgtccgaga cggcgcgtga gcgactgaac
    17581 gtgatgcgtc ggaccgagga cggtttcgtg atcgcggaag aggatttgcg gctccgcggc
    17641 ggcggcgaat tgctcggcac gcggcaaagc ggattgccga tgttccgcgt tgccaccctg
    17701 cccgagcatc aggcgctgct cgaggcggcg cgcgaccagg ccagactcac gctcgggcgc
    17761 gaccccgagc tgaagggtca ggggtccgag gcgttgcggc tcctgcttta tctcttcgag
    17821 cgtgacgacg cgatcaagct gatgcgcgcg ggctgacccg ctcgcgagcc tacgacgaat
    17881 cggctcgtta gaggtcgatg agggacacgc ccgctagatt ggcgagtcag gaatcttgag
    17941 cttcagcgcg gctgccttct cgcggacctc ggcttcggtg cgccccagaa aggcggcaat
    18001 gtgggcgata gtacgcccgt gctcgatcct gttttgtagg gagaacagtt cctgtttgga
    18061 ccactctttg gcgtccttga tgagattagg ctgttcggtc atgcgaccca ccccaactcc
    18121 cgggccttcg cgttcgcaag tctccacgct tgcgcgagaa actccgcctg agtcattggc
    18181 acgctactat catccttgct cgggaattta cgggcaagga gctggggaga gttatccggc
    18241 ttgtagtatg tcaccgtgta gctcgtgaag ggaacggtca cgacgatttc gtccgcattc
    18301 acgcttaagt cgaagtccga gcgcgacatt atcgcttcct catcccctgc ccgcgggcct
    18361 caatgcggtg catctagacc gaaaccctct agcatccggc tgcaaagctc tattgggtct
    18421 ctcacgccgg catcggccaa gtccatcagc ttttctgcca gggcttgctg cacttcggca
    18481 tctgtatccc aatcgcgatc gggatcgtgc gccttgagaa cttggcaaac gtctctcagc
    18541 gcttgttcaa gcgcgaccag ggttatctga tcctgacttt tcggcgtttt ctgagaagcc
    18601 attggctccc tcattgcgtt gcaggcggga gcgcgcgtta gctctcagtc agcggtgcct
    18661 gtcgtaatct ttgaccggtg atgagacgag aatggcacgt ctgttgccgc gagtctgcag
    18721 ccaagtcgac tccgtcgagt tggggcgcac tgctctctcc atgtgttcca ggcgggagcg
    18781 cgacctaaca ggcggtggtc ctaccgcacc gccggaggag gagcttcgcg cacatcaaaa
    18841 aatttggtag tcctggcagc aggataggat cgagcgatgc ccctcatacc gacggggccg
    18901 tttttgaggc ttatcgcgaa ctgattgagg atgttgcgag tggcgccttc gacgctgaag
    18961 ggcctttcga tgatcatggc a
//
LOCUS       DASUQP010000126        10915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1558462, whole genome shotgun sequence.
ACCESSION   DASUQP010000126 DASUQP010000000
VERSION     DASUQP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558462"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..643
                     /gene="ruvA"
                     /locus_tag="VFY74_05700"
     CDS             29..643
                     /gene="ruvA"
                     /locus_tag="VFY74_05700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5931229.1"
                     /translation="MIGKLTGRLECVSPSAAIVDVGGVGYEVTVGARTLAALPPPGEI
                     VTLAIDTHVREDEIRLYGFATEHERAWFRALQTVQGVGAKIALAVLGTLSAADLANAV
                     ALQDKAQVGRAPGVGPKVAARIVAELKDKMPSLAPAIRPGRGFAPVAVLPEGLAARDA
                     VSALTNLGYAHGEAAAAVTAAIGKAGREARAEELIRLGLRELAQ"
     gene            746..1789
                     /gene="ruvB"
                     /locus_tag="VFY74_05705"
     CDS             746..1789
                     /gene="ruvB"
                     /locus_tag="VFY74_05705"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041800114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5931230.1"
                     /translation="MSMTERMLQAVRRDEDGGETSLRPQTLSEFIGQAKVCSNLKVFI
                     DAARSRREALDHVLFAGPPGLGKTTLAQIVARELGVNFRATSGPVIAKAGDLAALLTN
                     LEDRDVLFIDEIHRLNPAVEEILYPAMEDYQLDLIIGEGPAARSVRIDLARFTLIGAT
                     TRTGLLTTPLRERFGIPIRLEFYGVEELEEIVRRGARLLGLSLTGEGAHEIARRARGT
                     PRVAGRLLRRVRDFACVEGVGAVDAGTADRALSKLEVDGRGLDALDHRYLNLIAVNYG
                     GGPVGVETIAAGLSEPRDAIEEIIEPYLLQQGFIARTPRGRVLTAQAFAHLGLPPPAQ
                     SAAQFALFNGSEE"
     gene            complement(1805..2206)
                     /locus_tag="VFY74_05710"
     CDS             complement(1805..2206)
                     /locus_tag="VFY74_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5931231.1"
                     /translation="MIDPRSLSNWTPYALAILRIVAGLMFLEHGTQKLFGFPPGERAF
                     VEAFTLSWWAGVLELILGALITVGLFTRAAAFVAAGEMAFAYWLAHAPQNFFPVNNGG
                     DAAILYCFVFLLLVFAGPGKWSLDGWREAET"
     gene            2296..2796
                     /gene="ybgC"
                     /locus_tag="VFY74_05715"
     CDS             2296..2796
                     /gene="ybgC"
                     /locus_tag="VFY74_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX5931232.1"
                     /translation="MSKTSESMRPSGEARWPDIAGRIEGETHVLPIRVYFEDTDCAGV
                     VYHANFLKFCERARSDFIRLLGIDAKSLAEPEHGEPTAFVVRRVEIDYLKPGHMDDLL
                     EIVTSCAEIGSASLVLQQDVRRDGAPLVRARVTVVLVSRAGKPQRLGALVRTALQRFV
                     NQQRET"
     gene            3024..3728
                     /gene="tolQ"
                     /locus_tag="VFY74_05720"
     CDS             3024..3728
                     /gene="tolQ"
                     /locus_tag="VFY74_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX5931233.1"
                     /translation="MNPPDVVVDLSILELFKNAHIVVKLVILGLLFASVWSWAIILEK
                     LFLYAKTRKETDKFEQVFWSGQSLDELYDALKQRRNTGMAAVFVAAMREWKRSTERES
                     GDRAAKPMPGVQLRVEKVMDVTVSREVERLERRLTFLATVGSTAPFVGLFGTVWGIMT
                     SFQAIAVSKNTSLAVVAPGIAEALFATALGLLAAIPAVIFYNKFNSEVARHAARLESF
                     ADEFGAILSRQIDKTT"
     gene            3738..4211
                     /locus_tag="VFY74_05725"
     CDS             3738..4211
                     /locus_tag="VFY74_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEX5931234.1"
                     /translation="MGAGLQPGGALSNRRARRRSASPMSEINVTPMVDVMLVLLIIFM
                     VTAPLLTVGVDVESKGTLPKVELPKTEAGAVEPERAPLSVTVDPQGKIFLQNTEIGFD
                     ELVPKLAAIRDAGGGSKVWVRGDKATAYANVAKVLARIGQAGFPVTIVTDQMDQP"
     gene            4238..5089
                     /locus_tag="VFY74_05730"
     CDS             4238..5089
                     /locus_tag="VFY74_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX5931235.1"
                     /translation="MGRSTGISAALHAAILFFALVAFPPAKMERAPLIAIPVDLATPS
                     EVTKIKAGSAGAKDEAPLTGKPKEKKPEAVKEAKKPSEQAAELAPAAEPQPKPEPKAK
                     TESSKSAAKKPDPKPAKPKQAAAPKKPAAPTGKKRDFDTDRIAALLNKIPDEANEPAP
                     VLASEEAPSQKVRGQSNGKDAAMSVNEIDALRARIAQCWSPPPGGLGAEEIVVKLRLK
                     LNEDGTLVGYPTVANNGASPFFQAAADSAVRAVYQCQPYTLPSDKYALWRDMILNFDP
                     SEMYRAG"
     gene            5122..6468
                     /gene="tolB"
                     /locus_tag="VFY74_05735"
     CDS             5122..6468
                     /gene="tolB"
                     /locus_tag="VFY74_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX5931236.1"
                     /translation="MTSTQNANPYRHCRFARFILVLLALGALSPSPAHAVVELNITQG
                     TIQPLPIAIADFVGDGSVAPDVAREVSGVVANDLRLSGLFIPIDQAAFIEKGVSPDHL
                     PRFEDWRVLNAQALVVGRIGASDGKLRAEFRLWDVFSGAQLAGEQFFARPKDARRIGH
                     IIADAIYERITGEKGYFDTRIVFVDESGPKNKRMKRLAIMDQDGFNVRLLSSGQNLVL
                     TPRFSPSNQEITYTSFEGETPKVYLLNIETGQKEIVGAFPGMTFAPRFSPDGQRIIMS
                     LERDGNSNLFVMDLRSRRKVQLTNNNAIDTAPCYAPDGRRIVFESDRGGSRQLYVMGA
                     DGSAPRRISQGGGSYSTPVWSPRGDLIAFTKMSGGQFLIGVMKPDGSGERILTEGFHN
                     EGPTWAPNGRVLMFFREQPGAAGGPRLFTIDLTGYNERQVATPAFGSDPAWSPLIN"
     gene            7115..7627
                     /gene="pal"
                     /locus_tag="VFY74_05740"
     CDS             7115..7627
                     /gene="pal"
                     /locus_tag="VFY74_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX5931237.1"
                     /translation="MRWSCGVARACKIAGVVALAIVLAACSRQAQPRQYGLGGEGPAT
                     PGTERDFAVNVGDVVRFEVDSSTLTGEAQGILQNQARWLNDYPQYGITIEGHADERGT
                     REYNLALGAKRAIAVRSFLASQGVRAPRIKTISYGKERPVAVCDDISCWSQNRRAQTV
                     LNSGAVAGNY"
     gene            7878..8873
                     /gene="ybgF"
                     /locus_tag="VFY74_05745"
     CDS             7878..8873
                     /gene="ybgF"
                     /locus_tag="VFY74_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX5931238.1"
                     /translation="MAGFLVAALPMPGLAQATQSGDSIGARLQRLEAKVQSLQVTIGT
                     LQSLSAARSAAPHPQDAPQPLAPPRPGSGDLGPRIEALEMQIAALTSHIEQIGKRMSA
                     LEAKLAGADAPAALTAPSVASPPAGQVEAPKSGLRAFFSSSEDSSDEPGDAGDSSGQR
                     SYGAEPGDDEVAKLLEDEAASQQARAGGDATPGAPQNLTPSLPGAEAETLYRQGHAAL
                     IQKDYAGAESAFARLIEANPNDPLAGKAQFWIGEAYYARSEYKGAADAFLKAYKNDES
                     AEKAPDALLKLGMALAGLGQKDAACSTFQELKTKFPGAPAPIPTEADSWRKKTGC"
     gene            8875..10194
                     /gene="tilS"
                     /locus_tag="VFY74_05750"
     CDS             8875..10194
                     /gene="tilS"
                     /locus_tag="VFY74_05750"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5931239.1"
                     /translation="MNASPISRLEAEKLFAPLAYFPRLALAVSGGPDSLALMLLVARW
                     REAREARPELSVLTVDHGLRASSREEAERVERMASSLGLGHAILTWDKAELPSARLQA
                     RARTARYDLMAAHCHAHGIPALVTAHTQDDQAETLLMRLKRGSGLDGLAAIPEKGSWA
                     GIAILRPLLDVPKARLLATLDDAGLHFISDPSNADPRFERARMRERTGALEALGLTPE
                     ALALSARRLRRSREALDAASRDFLARHSAMSETGYATLDRAALATAPHDIALRALAHL
                     IAAVGGGETPVQLAKLEKLLAALEASPDKAHTLGRCRLEPVSGRLGLFREVRAAGLPV
                     ARVRPGERALWDNRFTIELGANEPEAVTVRALGEPGLRELRDRLPLISSVPRLAGRTL
                     PSCWRGEVLLGLPALGPRAWSAPAGLDCRARFVGSGSDAWALERPRA"
     gene            10351..>10915
                     /locus_tag="VFY74_05755"
     CDS             10351..>10915
                     /locus_tag="VFY74_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX5931240.1"
                     /translation="MNSNFRNFAIWVFIGLLLVALFNLFQSPGVQVRGNEISFSQLLS
                     EVESGNVQDVTIAGNQITGHFSDGRNFQTYAPNDPNLVDKLNQKGVKITAKPSEDDVP
                     SLLGVLVSWFPMLLLIAVWVFFMRQMQTGGGRAMGFGKSKAKLLTEAHGRVTFDDVAG
                     VDEAKEEVAELVDFLRDPSKFQKLGGRI"
BASE COUNT         1977 a         3527 c         3535 g         1876 t
ORIGIN      
        1 cgatcgccgc acaggagggg gccttgtcat gatcggcaag ctcaccgggc ggctcgagtg
       61 cgtctcgccg agcgcggcga tcgtcgatgt cggcggtgtg ggctacgagg tcacggtcgg
      121 cgcccgcacg ctcgccgccc tgccgccgcc gggcgagatc gtgacgctcg ctatcgacac
      181 ccatgtgcgg gaggatgaga tcaggctcta cggctttgcc accgagcatg agcgtgcctg
      241 gtttagggcg ctccagacgg tgcagggggt gggcgcgaag atcgcgctcg ccgtgctcgg
      301 cacgctttcg gccgcggacc tcgccaatgc cgtggccctt caggacaagg cgcaggtcgg
      361 ccgggcgccc ggcgtcggcc ccaaggttgc agcccggatc gtggcggagc tgaaagacaa
      421 gatgccgtcg cttgcgccgg cgatcaggcc ggggcgggga ttcgctcccg ttgcggttct
      481 gcccgaaggg ctcgccgcgc gcgacgcggt gtcggcgctc accaatctcg gctatgccca
      541 tggcgaggcc gcggccgccg tcaccgcggc gatcggcaag gccgggcgcg aagcccgcgc
      601 cgaggagctg atccgcctcg gccttaggga gcttgcgcaa tgaacggccg gagcttccca
      661 gagattgcgc ccgcccctcc tatccctccc cacgttgggg gagggatgaa gcacgcggca
      721 aggcacagcg acatggcccg gaggcatttc catgactgag aggatgctcc aggcggtgcg
      781 gcgcgacgag gatggcggcg agacctcgct ccggccgcag acgctctccg agttcatcgg
      841 acaggccaag gtctgctcca acctcaaagt gttcatcgat gcggcccgct cgcgccgcga
      901 ggcgctcgac catgtgctgt tcgcagggcc ccccggtctc ggcaagacca cgctcgctca
      961 gatcgtggcg cgcgagcttg gcgtcaattt ccgcgcgacc tcgggccccg tcatcgccaa
     1021 ggccggcgat ctcgcggcac ttctgaccaa tctcgaagac cgcgatgtcc tcttcatcga
     1081 cgagatccac cggctcaatc ccgcggtcga agaaatcctc tatccggcga tggaggacta
     1141 ccagctcgac ctcatcatcg gcgaaggacc cgccgcgcgc tcggtgcgga tcgatcttgc
     1201 ccgcttcacc ctgatcggcg ccacaacgcg cacaggactt ctcaccacgc cgctcaggga
     1261 gcggttcggc attccgatca ggctcgaatt ctatggcgtc gaggagctcg aggagatcgt
     1321 gcggcgcggc gcccggctgc ttgggctttc gctgaccggc gagggcgcgc acgagatcgc
     1381 gcggcgcgcg cggggcacgc cgcgggtcgc gggcaggctg cttcgccgcg tgcgcgactt
     1441 cgcctgcgtc gaaggagtag gcgcggtcga tgccggaacc gccgaccgcg cgctgtcgaa
     1501 gcttgaggtg gacggcagag gattagatgc gctggaccac cgctacctta atttgatcgc
     1561 ggtcaactac gggggcggtc cggtcggggt cgagaccatc gccgccggcc tctcggagcc
     1621 gcgcgacgcc atcgaggaga tcatcgagcc ttatctgttg cagcagggct tcatcgcgcg
     1681 caccccgcgg ggccgagtgc tgacggcgca ggctttcgcc catctcggcc tgccgcctcc
     1741 ggctcaatcg gcggcgcagt ttgccctgtt caacggtagc gaggaatagg cgtcgttggc
     1801 ttgcctatgt ctctgcttca cgccagccgt ccaagctcca ttttccaggg cccgcgaaca
     1861 cgagcaacag gaacacgaag caatagagaa tggccgcgtc accgccattg tttacgggga
     1921 agaaattttg cggcgcgtgc gcgagccaat aggcgaaggc catctcgccc gccgcgacaa
     1981 aggcagcggc tcgcgtgaag agcccgacag tgatcaaggc ccccaagatg agttcgagga
     2041 cgcccgccca ccacgacagt gtgaacgcct cgacgaaagc ccgttcgccc ggagggaatc
     2101 cgaagagctt ctgcgtcccg tgctccaaaa acataagccc ggcgacgatt ctcagaatgg
     2161 caagagcgta aggggtccag ttggacagac ttctgggatc gatcatattt cctcccgtgt
     2221 gccagcgtgc cagcaatcct cggagtttgc acgatgcgtg gcaagcgtcg gcttttggaa
     2281 ggtatttgaa tccgcatgag caagacatcc gagagcatga ggccaagcgg cgaggcgcga
     2341 tggcccgata tcgccggccg catcgagggc gagacccacg tgctgccaat cagagtctat
     2401 ttcgaggaca ccgactgcgc cggcgtcgtc tatcacgcca acttcctcaa attctgcgag
     2461 cgcgcacgct ccgatttcat caggctgctc ggcatcgacg cgaaaagcct cgccgaacca
     2521 gagcatggcg agccgacagc gttcgtcgtg cgccgggtcg agatcgacta tctcaagccc
     2581 ggccacatgg acgatctgct cgagatcgtc acgtcttgcg ccgaaatcgg cagcgcgagc
     2641 ctcgtgctgc agcaggacgt gcggcgggat ggggctccgc tcgtccgcgc cagagtgacc
     2701 gtcgttctcg tctcgcgggc gggcaagccg cagcggttgg gggcgctggt gcgcaccgcg
     2761 cttcaacgat tcgttaacca acagcgtgaa acgtagggga tgccggtaaa gcgtcgtttt
     2821 gcttgagcgc cgtgcgcgcc gcgcgtgagg gtaaacgggt ccaagtttcg tcaccatcgg
     2881 ctcgcaaaag gccggtgcgc gagcataacg cacgctctcc ccggcgatgt cgtaaggctt
     2941 gcggcgaaag ccgcgggcca aagcgcttgc ctcatggctg atgccggagg ctgcgatctt
     3001 ggagctcatg ccacgaggcc gtcatgaatc cgccggatgt cgtcgtcgat ctgtccattc
     3061 ttgagctgtt caagaacgcg catatcgtcg tcaagctcgt catcctcggg cttctgttcg
     3121 cctcggtctg gagctgggcg atcatcctcg agaagctgtt cctctacgcc aagacgcgaa
     3181 aagagaccga caagttcgag caggtgttct ggtccggaca atcgctcgat gagctctacg
     3241 acgcgctgaa gcagcgccgg aacacgggaa tggcggccgt gttcgtggcg gcgatgcgcg
     3301 aatggaagcg ctcgaccgag cgcgagagcg gcgaccgtgc cgcaaagccc atgcccggcg
     3361 tccagctcag ggtcgagaag gtcatggacg tgaccgtctc ccgagaggtg gaacggctcg
     3421 agcgccgctt gaccttcctc gccacggtcg gctcgaccgc ccccttcgtc ggcctgttcg
     3481 ggacggtgtg gggcatcatg acgagcttcc aggcgattgc cgtgagcaag aacacgagcc
     3541 tcgccgtggt cgcgccgggc atcgccgaag ccctgtttgc cactgcgctc ggcctgctcg
     3601 ccgccattcc ggccgtaatt ttctacaaca agttcaatag cgaagttgcg cgccacgcgg
     3661 cccgtctcga aagtttcgcc gatgagttcg gggcgatcct ttcgcggcaa atcgacaaga
     3721 cgacgtgagg ccaagccatg ggagcaggtt tacagccagg aggcgcgctc tcgaatcgga
     3781 gagcgcggcg ccgctcggct tcgccgatga gcgagatcaa cgtcacgccc atggtcgacg
     3841 tcatgctcgt gttgctcatc atcttcatgg tgacggcgcc gcttctcacc gtcggtgtcg
     3901 acgtcgagtc gaagggaacg ttgcccaagg tcgagctgcc gaagaccgag gccggcgcgg
     3961 tcgagccgga aagggcgcca ttgagcgtga ccgtggatcc ccaagggaag atcttcctgc
     4021 aaaacaccga gatcggcttt gacgaactcg tgcccaaact cgccgcgatc cgggacgcgg
     4081 gcggcggcag caaggtttgg gtgcgcgggg acaaggccac cgcttacgcc aatgtcgcga
     4141 aagtgctggc aaggatcggg caagcaggtt tccccgtgac gatcgtcacc gaccagatgg
     4201 atcagcctta agagggggtg gcggaagcaa ggcgatcatg ggcaggagca caggcatatc
     4261 ggcggcgctg catgcggcga tcctgttctt tgcgctcgtc gcctttccgc ctgccaagat
     4321 ggagcgggcg ccgctcatcg ccatccccgt ggatctcgca acgccctccg aggtgaccaa
     4381 gatcaaggcg ggaagcgccg gcgccaagga cgaggcgccg ctcacaggca agcccaaaga
     4441 gaagaagccg gaagcggtga aggaggcgaa gaagccttcc gagcaggccg cggaactcgc
     4501 tccggcagcg gagcctcagc cgaagcccga gccgaaagcg aagaccgagt cttctaagtc
     4561 cgcggccaag aagcccgatc caaaaccggc gaagccgaag caggccgccg cgccgaaaaa
     4621 gccggcagcg cccaccggaa agaagcgcga cttcgacacc gaccggattg ccgccttgct
     4681 gaacaagatt cccgacgagg cgaacgagcc ggcaccggtg ctggcctccg aagaggcgcc
     4741 aagccagaag gtgcggggac agtcgaacgg caaagacgcc gccatgtcgg tcaatgagat
     4801 cgacgcgctc agggcccgca tcgcccaatg ctggagcccg ccgccgggtg gcctcggcgc
     4861 cgaagagatc gtggtgaagc taaggctcaa gctcaacgag gacggcacgc tcgtcggcta
     4921 cccgaccgtc gccaataacg gcgcgtcgcc cttcttccag gccgcggccg atagcgcggt
     4981 gcgagccgtc tatcaatgcc agccctacac gctgccaagc gacaaatatg cgctctggcg
     5041 cgacatgatc ctcaatttcg acccaagcga gatgtatcgc gcgggctagc aagcctccgc
     5101 cttgccgatc gaagatccgc catgacctcg actcaaaacg ccaacccgta ccgacactgc
     5161 cgcttcgcgc gtttcatcct tgtcctcctc gcgctcggcg cgctgtcgcc aagcccggcg
     5221 cacgcggtcg tcgagctcaa catcacgcaa ggcacgatcc agccgctccc gatcgccatt
     5281 gccgactttg tcggcgacgg ctccgtggcc cccgacgtcg cgcgcgaggt ttccggcgtc
     5341 gtggcgaacg atctcagatt atccggcctg ttcattccga tcgaccaagc ggctttcatc
     5401 gagaaagggg ttagccccga tcatctgccg cgtttcgagg attggcgcgt gctcaacgcg
     5461 caagctcttg tcgtcggccg catcggtgca agcgacggca agctcagggc cgagttccgt
     5521 ttgtgggatg tcttctcagg cgcgcagctc gcgggcgagc agttcttcgc ccggcccaag
     5581 gatgcgcgcc gcatcggcca tatcattgcc gacgccatct acgagcggat cacgggcgag
     5641 aagggctact tcgacacccg catcgtgttc gtggacgagt cgggccccaa gaacaagcgg
     5701 atgaagcgcc tcgcgatcat ggatcaggac ggcttcaatg tgaggctgct gagctcggga
     5761 cagaatctcg tgctgacgcc gcgcttcagc ccctcgaacc aggaaatcac ctacacctcc
     5821 ttcgagggcg aaacgccgaa ggtctacttg ctcaatatcg agacgggcca gaaggagatt
     5881 gtcggcgcgt ttccgggcat gacgttcgcg ccgcgcttct cgcccgacgg tcaacgcatc
     5941 atcatgagcc tggagcgaga cgggaattcc aacctgttcg tcatggatct gcgcagccgg
     6001 cgcaaggtcc agctcaccaa taataatgcg atcgacacgg caccctgcta tgcgcccgat
     6061 ggacgccgga tcgtgtttga gagcgaccgc ggcggttcgc gccagcttta cgtcatgggc
     6121 gccgacggct ccgctccgcg ccgcatcagc caaggcgggg gaagctactc gacgcccgtc
     6181 tggtctccgc gcggcgacct gatcgccttc acgaagatga gcggggggca attcctgatc
     6241 ggggtgatga agccggacgg ctccggcgag cgaatattga ccgaggggtt ccacaatgaa
     6301 ggccccacat gggccccgaa cggcagggtg ctgatgttct tccgggagca gcccggggct
     6361 gcgggcggcc cgaggctctt caccatcgat ctcaccggat acaacgagcg gcaagtggcc
     6421 acgcccgctt tcggctccga cccagcctgg tcgcccctca tcaattgagc gcggaagttg
     6481 cgaaaatcaa atggtctcaa cgtttacccc aaccctcgcg ctcgcttaca gtttgttaag
     6541 cataacgcat cagactgtta accgatcttg attggcccct gaaccggggc taaatttggt
     6601 ggcagggact tcgggttggc gtggcatttt ggcaccaatc gcccggaaat cggtcgaggg
     6661 ccaagagtcc gttcttgatc tgcaaacctc ccgccggtac atgcaaagac ggctattgcc
     6721 gttttaacca aaggagagaa gaccatgcag ggtttgaagg gcgccatcat cgtcgccatg
     6781 atcgtgacca caagcatcgt gcttggtggc tgctggggac accacgagaa agccgtcgtt
     6841 gctgagcccc tgaagctcgg ctaagccgac accgtggtcg agcaggttgt tcgcgtttaa
     6901 gcttcggccg atagatagcg gaagcgctta gagccttccg cgttatggaa attggatgcc
     6961 gggacttcgc cgcgcaagcg gggtcgtccc aggcatcctt tccttttttg ggcgtttgcc
     7021 ttcccaattc gctcgatcgc gcccgatact aggacatcgt taagaactcc cgctctacga
     7081 tttcctaaaa ttcgacgagc gggggtaacc agtcatgcgt tggagttgtg gggttgcacg
     7141 cgcctgcaag atcgcgggcg tcgtcgcatt ggcgatcgtg ctggcggcct gttcgcgtca
     7201 agcgcagcca agacaatacg ggctcggcgg ggaagggccg gcaacgccgg gaacggaacg
     7261 ggattttgcc gtcaatgtcg gcgacgtggt gcgtttcgag gtggacagtt cgaccttgac
     7321 gggcgaggcg caagggatcc tgcaaaacca agcgcgttgg cttaacgact atccgcaata
     7381 cggcatcacg atcgaaggtc atgccgatga gcgcggcaca cgcgaatata acctcgcgct
     7441 cggtgccaag cgggccatag cggtgagaag cttcttggcg agccaggggg tcagggcacc
     7501 gcgcatcaag accatctctt atggcaagga gcggcctgtc gcggtctgtg acgacatctc
     7561 ctgctggtcg cagaatcggc gcgcgcaaac cgtgctgaac agcggcgccg tcgccggcaa
     7621 ttactagccg tcacgcgact gctggggatt tcccggcggt tcttccggcg cccgtctgcg
     7681 ctctcgggag cttgcctgtt tgagaaaggc cgcagtttgg ccccaaaccg ccgcccaaat
     7741 gggccagcac cgggtcgccc ctggacggcc cggctcgcaa tgctaaccca catcgttgcg
     7801 gcactcacgg cgaggtcctc ctcgtgtcat cttcggtttt cacgcggcgc aagcgatggc
     7861 gcccgacttg cgttttgatg gcagggtttc ttgttgccgc cctgccgatg cctggcttgg
     7921 cgcaagcgac ccaaagcggc gattcgatcg gtgcccggct ccagcgcctc gaagccaagg
     7981 tccagagcct gcaagtcacg atcggcacgc tccaatcgtt gagcgcggca agatccgccg
     8041 caccgcatcc gcaagacgcg cctcaacctc tcgcgccgcc tcgacccgga tcgggcgatc
     8101 tcggaccgcg catcgaggcg ctcgaaatgc agatcgcggc gctcacaagc catatcgagc
     8161 agatcggaaa gcggatgagc gcgctcgagg cgaagctcgc cggggcggat gcccccgccg
     8221 cgctgacggc gccgagcgtg gcatcgccgc ccgcaggaca ggttgaggcg ccgaaatcgg
     8281 gtctcagagc gttcttctcc tcttccgaag attcttcgga cgaacccggc gatgcgggcg
     8341 atagctcagg acagcgctcg tacggtgctg agcctggcga cgacgaggtg gcgaagctgc
     8401 tcgaagatga ggccgcatcg cagcaggcgc gagcgggagg ggatgcaact cccggtgctc
     8461 cgcaaaacct cacgccgtcg ctgccaggcg ccgaagcgga gacgctctac cgtcaagggc
     8521 atgccgcgct catccagaag gattatgctg gcgccgaaag cgccttcgcc cggttgatcg
     8581 aagcaaatcc gaacgatccg ttggcgggga aggcgcaatt ctggatcggc gaggcgtact
     8641 acgcgcgcag cgagtacaag ggcgccgccg atgccttcct caaggcctat aagaacgacg
     8701 aatcggcgga gaaggcgccg gacgcgttgc tcaagctcgg catggcgctc gccgggttag
     8761 gccagaagga cgcggcctgc tcgacatttc aggaattgaa gaccaaattc ccgggtgcgc
     8821 cggcgccgat ccccacggag gccgacagct ggcgcaagaa gaccggctgc tgacatgaac
     8881 gcttcaccca tctcgcgctt ggaggccgaa aagctcttcg cgcccttggc gtactttccg
     8941 cgccttgcgc tcgccgtgtc gggcggaccg gacagcctcg cgctcatgct cctcgtggcg
     9001 cgctggcgtg aagcacgcga agcacgcccc gagctcagcg tgctcaccgt cgaccacggg
     9061 cttcgcgcaa gctcgcgcga agaggcggaa cgcgtcgagc gtatggcttc gtcgcttggc
     9121 ctcggccacg cgatcttgac ttgggacaag gctgagcttc cgtctgcgcg ccttcaggcg
     9181 cgggcgcgga ccgcgcgcta cgatctcatg gcggcgcatt gccacgccca cggcattccg
     9241 gccctcgtca ccgcccacac gcaagacgat caggccgaga ccttgctgat gcggctcaag
     9301 cgcggcagtg gcctcgatgg tttggcggca atcccggaga aaggctcatg ggcgggcatt
     9361 gcgatcttgc ggcccttgct cgacgtgccg aaagcacggc tcctcgccac gcttgatgat
     9421 gcgggcctcc actttatctc agatccgagc aatgccgatc cgcgcttcga gcgtgcgcgg
     9481 atgcgggagc gcaccggcgc gctcgaagct ctcggattga cgccggaagc gcttgcgctg
     9541 tcggcgcgcc gcttgcggcg atcccgcgag gcgctcgatg cggcctcgcg cgatttcctc
     9601 gcgcgccaca gcgccatgag cgagacaggc tacgccacgc tcgatcgcgc ggcgctcgcg
     9661 acggcgccgc acgacatcgc gcttcgcgcg ctcgcgcatc tgatcgccgc ggtcggcggc
     9721 ggcgagacgc cggtgcaact tgccaagctc gagaagctcc ttgccgcgtt ggaggcaagc
     9781 ccggacaagg cgcacacgct cggacgctgc cggctcgagc ccgtctccgg ccgcctcggc
     9841 ctcttccgcg aggtgagggc tgcgggactt cccgtcgccc gcgttcgtcc cggtgagcgg
     9901 gcgctgtggg acaaccgttt cacgatcgag cttggcgcaa acgagccgga agccgtcacc
     9961 gtgagggccc tgggcgagcc gggcttgcgt gagctccgcg accgcttgcc cctgatttcg
    10021 tcggtgccgc gcctcgccgg ccgcacgctt ccctcttgtt ggcggggcga ggtgcttctc
    10081 gggcttccgg cccttgggcc tcgagcctgg agcgcacccg ccggcctcga ttgccgggcg
    10141 cgcttcgtcg gttcagggtc tgacgcctgg gcgctggaac ggccacgcgc ataagccgtt
    10201 gacaaagcgt gcacctcttt cggttacttc gcttggcaaa gcggctcaag gttcttatgt
    10261 taccaaggct tagcctcaat ccgcccttat gctgcgaggc gaggctgccc ccatggtagc
    10321 cgcgatcaag tcgcgtcgtc aggacagtca atgaattcga atttccgcaa cttcgccatc
    10381 tgggtgttca tcggccttct tctggtcgcg ctgttcaacc tgttccagag cccgggcgtt
    10441 caggtccgcg gcaacgagat cagcttctcc cagcttcttt ccgaggtcga gagcggcaac
    10501 gttcaggatg tgaccatcgc cggcaatcag atcaccggac atttctccga tggccgcaac
    10561 ttccagacct acgcgccgaa cgatccgaac ctggtcgaca agttgaacca gaagggcgtg
    10621 aagatcacgg cgaagccgtc cgaggacgat gttccgtctc tgctcggcgt gctggtgtcg
    10681 tggttcccga tgctgctcct catcgccgtg tgggtcttct tcatgcgcca gatgcaaacg
    10741 ggcggcggcc gcgccatggg cttcggcaag tccaaggcga agcttctcac ggaggcccac
    10801 ggccgcgtga cgttcgatga cgtcgcgggc gtggacgagg ccaaggagga agtggccgag
    10861 ctggtcgact tcctgcgcga tccgtcgaag ttccagaagc tcggtggccg catcc
//
LOCUS       DASUQP010000127         7409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_60332,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000127 DASUQP010000000
VERSION     DASUQP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60332"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..786
                     /locus_tag="VFY74_05760"
     CDS             97..786
                     /locus_tag="VFY74_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX5931241.1"
                     /translation="MNKADLGYRASLAFQSGPDESRSGLPAAGISCSSQPSAAMINPA
                     IALLLTRRSVSANSLTDPGPNAEELELMLRAATRVPDHKKLLPWRFLLFQGEARAAFG
                     KALAEICREEQNDTGAFRLENEEKRFLRAPLVIAVISRVVKNPGAPEWEQILSVGAAC
                     QNLILAATALGYGANWITEWYGYSEGVRRLLRLAPNERVAGFVYVGTAREKPDERERP
                     NLADIIAAWCP"
     gene            complement(1174..1944)
                     /locus_tag="VFY74_05765"
     CDS             complement(1174..1944)
                     /locus_tag="VFY74_05765"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX5931242.1"
                     /translation="MPRFDKIAFVASETKEAQEALQALNKRYGSAPAAKADVIVALGG
                     DGLMLQTMHRHLNSRIPIYGMNRGSVGFLLNDYHEDRLGERLEAAETTVIHPLRMTAH
                     DRHGATHEALAINEVSLFRQIYQAAKLRIAVDGKVRMDELICDGVLVSTPAGSTAYNL
                     SAHGPILPINAPLLAMTPISPFRPRRWRGALLSNDAKVVIEVLEPEKRPVSAVADHTE
                     FRDVVRVEVVEESGIDIFMMFDPGHTLAERILSEQFGY"
     gene            2048..2389
                     /gene="cutA"
                     /locus_tag="VFY74_05770"
     CDS             2048..2389
                     /gene="cutA"
                     /locus_tag="VFY74_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX5931243.1"
                     /translation="MANEDNPVLIYTTFPGLDDARRIGEALVAARLAACVNMFPGMIS
                     IFEWKGAREEASEVAMIVKTRRGLTEAVLAEVKRLHPYELPALLVLPTEGGSAAYCGW
                     IASETASAGRK"
     gene            2386..3897
                     /gene="glpK"
                     /locus_tag="VFY74_05775"
     CDS             2386..3897
                     /gene="glpK"
                     /locus_tag="VFY74_05775"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX5931244.1"
                     /translation="MSGEAFVLAIDQGTTSTRAIVFDREGRPRASAQVPLTQIYPAPG
                     QVEHDPEEIWRSVLHVGRAALGDIEPNRIAGVGITNQRETTVVWERATGRPLADAIVW
                     QDRRTAALCEALAGEGWGDHVAQATGLVIDPYFSATKLAWLLQNVPGLEKRARAGEVC
                     FGTVDSFLLFRLTGGGLHATDATNAARTMLYDIRSGEWDGRLLGRLGVPRAMLPEVRD
                     TQGDFGPTVPEHFGAAIPIRGIAGDQQAAAYGQACFAPGMLKATFGTGCFVLTNTGAE
                     KVASATRMLSTIFHQISGKRTYALEGAIFMAGATVQWLRDNLGLIASAAESEVLAREA
                     DPNARVYLIPAFQGLGAPFWDAGARGAILGLSRAASKADLVAAGLEAVAFQTRDLLSA
                     MRQDMEARGIASPSPLRVDGGMTANSWFLQRLADILGETVELASNPETTALGAAYHAG
                     QATGFFGDAGTLAKAWVPARAFAPIMSDTEREDRYGGWLDAVARVRSGGADIP"
     gene            4000..4365
                     /locus_tag="VFY74_05780"
     CDS             4000..4365
                     /locus_tag="VFY74_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5931245.1"
                     /translation="MTMAAIDFQLLANAGFPPVTFNPGDVIFKTGDKGDKMYVVRAGE
                     VEIERDGKIVETVSSGGIFGEMALIDGAPRAATARAKTACEVAPVSEKTFLFLVHETP
                     YFAISVMRTLADRLRRSQP"
     gene            4534..6684
                     /locus_tag="VFY74_05785"
     CDS             4534..6684
                     /locus_tag="VFY74_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5931246.1"
                     /translation="MAGVISAVVQIAYCISFAALIFTGDLAGGFPLGLAGLIMGTVVT
                     CVVIAITSTFSPVIGGPDSPAVAVMSVLAASIATALAAKGASTDQIIVNVLVALSVST
                     LLTGILLCGVGTLRLGQWLRFIPYPVVGGFLAASGLLLITGGMEVVTQTDLTLLPSSW
                     ALLYSPLYAPQIVVAMLFAVAIPVMARFVPAYLALPLVFFAFLIVLNAGLFGVVGDDG
                     VRGAWFLPELGELTLWWPINAMIGDQIDWGVIARSSAEIGSFCGVMAIALLLDVSSLE
                     VARQKSGDLDQEFRSNGLANLLASVLGGFGGSLSMNACLLLDESGATTRWAGAIVGLV
                     CALILFSGLDVGSIVPKAILAGMLIYLGAVILIELWNAPAESSWMEWGLTGIMTLVII
                     NLGYFMGVVLGVIGACLMFALSYSRIGVIRRHLTRQEFSSNVERSPEQSRLLREEGKR
                     VHVFWLSGFIFFGSSNGLFERIKRVIEAQREKPVAFVVLDFGGVPGLDTSAVLSLVKL
                     RNFCEEHDVTLGFSGLSESMKASFERAGFFGSTRAHQVFGSRNEAVEWCEDMLLMHHE
                     VGEMSTHSFETWLESEFGGTVDFARIASFMERQELNNGAFLFRQGEAADSIVLQASGS
                     VAITIIDEHGRPIRLRRMVGHTVVGEMGFYRGVPRTASVIAEGPTVIYRLTRKAFDRM
                     QEEDPAAAAALHKLIIRLLSDRLEFANREISALL"
     gene            complement(6681..7073)
                     /locus_tag="VFY74_05790"
     CDS             complement(6681..7073)
                     /locus_tag="VFY74_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEX5931247.1"
                     /translation="MPDLALVALAAVAVLLVKHAVADFYLQTPYQYLNKGIYGHPGGL
                     LHSGIHVALTPLVYLVLVPGSLLVAGAIAVSEFLLHYHIDWLKEQVIQRNGLTAQDPG
                     FWHALGTDQLIHGLTYLAIVAALLATAP"
     gene            complement(7079..>7409)
                     /locus_tag="VFY74_05795"
     CDS             complement(7079..>7409)
                     /locus_tag="VFY74_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5931248.1"
                     /translation="VRAPDFAFLELDLLKVKGKTEATRVFALLGDSAFKQSQDFIRLA
                     ERHGAFLARYRAKEWDAAEALSRECEKLNTSRLDKLYALYRERIAYFRVNPPPPQWDG
                     TAEALSK"
BASE COUNT         1225 a         2369 c         2462 g         1353 t
ORIGIN      
        1 gagtccacgt atgccgcggc gcgtcccccg cattccctcg ctccgatccg acggccgcag
       61 agctgttata ccccgatttg agggcgaaaa tcctttgtga ataaggccga tttggggtac
      121 cgggcctctc tcgccttcca atccggtccg gacgagtcgc gttccgggct gcccgccgcc
      181 gggatcagct gctcctccca accctccgcc gccatgatca accctgccat cgcgctcctg
      241 ctgacccgtc gttcggtctc cgccaacagc ctcaccgatc ccggtcccaa tgctgaggaa
      301 cttgaattga tgctgagggc ggccacgcgc gtgcccgacc acaagaagct cctgccctgg
      361 cgcttcctcc tgtttcaggg cgaggcgcgc gccgcgttcg gaaaggctct cgccgaaatc
      421 tgccgtgaag agcagaatga cacgggggcc ttccggctcg aaaacgaaga gaagcgcttc
      481 ttgcgcgcgc cgcttgtcat cgcggtgatc tcgcgcgtgg tgaagaaccc gggggcgccc
      541 gagtgggagc aaatcctttc ggtcggcgcc gcctgccaga atctcattct tgccgccacc
      601 gcgctcggct acggcgcgaa ttggatcacc gagtggtacg gctatagcga gggcgtgcgc
      661 aggctccttc ggctggcccc gaacgagcgc gtggcgggct tcgtctatgt cggcacggcg
      721 agagaaaagc ccgacgagcg ggagcggccg aacctcgccg acatcatcgc ggcttggtgt
      781 ccataggatg cgtgggaaac gacgtgaatg gcggctttct tgtttcgacg cgggcttcgc
      841 ccgcttcccc ctcaccgcgc tgacgcgcgc gtgctctccc caaggggaga gcggaagaag
      901 cggtgcaaac tacaaccctc atgctgagga gcgcgcggcg gcccgccgcc agcgcgcgtc
      961 tcgaagcacg cacttggctt taagccatcc cctcgagacc gacgcgttcc cgcgccctcc
     1021 tcaaggtgag gacataaaga acgcaagaac gcggggcaca caaaacccgt catgctgagg
     1081 agcgcccgca gggcgcctcg aagcacgcgc gcccttcgag gggccttcgg ccctcctcag
     1141 ggtgacgatg cccagttcgt tcgggttgga agatcagtag ccgaactgct cggagagaat
     1201 gcgctcggcg agcgtatgac ccggatcgaa catcatgaaa atgtcgatgc cgctctcctc
     1261 gaccacctcg accctgacca catcgcgaaa ctcggtgtgg tcggcgaccg cgctcacggg
     1321 gcgcttctcg ggctcgagca cctcgatgac gaccttggcg tcattggaga ggagggcgcc
     1381 gcgccagcgc cgcgggcgga acgggctgat cggcgtcatg gccagcagcg gcgcgttgat
     1441 cggaaggatc ggaccgtgag cggacaggtt ataggcggtg ctgcctgccg gcgtcgaaac
     1501 gagcacgccg tcgcagatga gctcgtccat gcggaccttg ccgtccaccg cgatcctgag
     1561 cttggcggcc tgatagatct gccggaacag cgagacttcg ttgatcgcga gcgcttcgtg
     1621 cgtcgcgccg tggcggtcgt gggcggtcat acgcagaggg tgaatgacgg ttgtctcggc
     1681 cgcttccagc cgctcgccaa gcctgtcctc atgatagtcg ttgagcagaa agccgacgga
     1741 gccgcggttc atgccgtaga tcggaatgcg gctgttgaga tgacggtgca tggtttgcag
     1801 catcagcccg tcgccgccga gcgccacgat gacgtcggcc ttcgccgccg gagcgctgcc
     1861 gtagcgtttg ttgagcgcct gaagcgcctc ttgtgcctct ttggtctcgc tggcaacgaa
     1921 ggcgattttg tcgaagcggg gcatgatcgg gtcgaggcgc ttggagcggg gtcaagaagc
     1981 gggcactata aacgcgagcg cgccccgcaa aagcaagtgc ttagaaatga cagcgcatgg
     2041 tgaattcatg gcgaacgaag acaaccccgt cctgatctat acgaccttcc ccggcctcga
     2101 cgacgcaaga cggatcgggg aggcgctggt ggcggcccgg cttgccgcgt gcgtcaacat
     2161 gttcccgggc atgatctcca tcttcgagtg gaagggggct cgggaggagg cgagcgaggt
     2221 cgccatgatc gtcaagacgc ggcgcggatt gaccgaggcc gtgctcgcgg aggtgaagcg
     2281 gctccatccg tacgagctgc cggcgctgct cgtgctgccg acggaaggcg gcagcgccgc
     2341 atattgcggc tggatcgcaa gcgaaacggc aagcgcaggg cgcaaatgag cggggaggcc
     2401 ttcgtcctcg ccatcgatca gggaacgacg agcacccgtg ccatcgtgtt cgaccgcgag
     2461 gggcgccccc gggcaagcgc gcaagtgccg ctcacgcaga tctatccggc gccgggccaa
     2521 gtcgagcacg atcccgagga gatctggcgg tccgtgcttc acgtcggccg cgccgcgctc
     2581 ggcgacatcg agccgaacag gatcgcggga gtcggcatca ccaatcagcg cgagacgacg
     2641 gtcgtctggg agcgcgcgac ggggaggccg ctcgccgatg ccatcgtgtg gcaggaccgc
     2701 cgcaccgccg cgctttgcga ggcgcttgcg ggcgaggggt ggggcgatca tgtcgcgcaa
     2761 gcgaccggac tcgtgatcga cccttacttc tccgcgacca agctcgcctg gctgcttcag
     2821 aacgtaccgg ggctcgaaaa gcgggcgcgt gccggcgaag tgtgtttcgg aacggtcgac
     2881 agctttctcc tgttcaggct gaccggcggc gggttgcacg cgaccgacgc gaccaacgcc
     2941 gcccgcacca tgctctatga catcagatcg ggagagtggg atggaaggct gctcggccgg
     3001 ctcggcgtgc cgcgcgcgat gctgccggag gtgcgcgaca cgcaaggcga tttcggtccg
     3061 acggtacctg agcatttcgg cgccgcgatc ccgatcaggg gcatcgccgg cgaccagcag
     3121 gcggccgcct acggacaggc gtgcttcgcg cccggcatgc tcaaggcgac ctttggcacc
     3181 ggctgcttcg tgctcaccaa caccggcgcg gagaaggtcg cgtcggccac gcgcatgctc
     3241 tcgacgatct ttcatcagat ttctgggaag cggacctatg cgcttgaggg cgcgatcttc
     3301 atggcgggcg ccacggtgca gtggctcagg gacaatctcg gcctgatcgc gtcggcggcc
     3361 gagagcgagg tcttggcgcg cgaggccgat ccaaacgcgc gcgtgtatct catcccggcc
     3421 ttccagggac tgggcgcgcc gttctgggat gccggcgcgc gcggcgcgat cctcggattg
     3481 agccgcgccg cgtcgaaggc cgatctcgtg gcggcgggcc tcgaggccgt cgccttccag
     3541 acccgcgatc tcctttccgc catgcgccaa gacatggagg cgcggggcat cgcttcgccc
     3601 tcgcccttgc gggtcgatgg gggcatgacg gcgaattcgt ggtttctgca aaggcttgcc
     3661 gacattcttg gcgagaccgt cgagctcgcg tcaaaccccg agaccacggc gctcggtgcc
     3721 gcttaccacg cgggacaggc gaccggcttt ttcggcgatg ccggaacgct ggcgaaggcg
     3781 tgggtgccgg cgcgcgcgtt cgcgccgatc atgtccgaca ccgagcgcga ggatcgttac
     3841 ggcggctggc tcgacgcggt cgcccgcgtc aggtccggcg gcgccgacat tccctgagca
     3901 ctcccgccgt cggctcgttt gcctgggcgc aaagcctcat agtactgtca tcaaaccatc
     3961 atagagttgc cggagatcgc gaacggagag ggcacgtcca tgacgatggc tgcaatcgac
     4021 ttccaacttc tcgccaacgc gggatttcct cccgtgacct tcaaccccgg cgacgtcatc
     4081 ttcaagaccg gtgacaaggg cgacaagatg tacgtggtgc gcgcaggcga ggtcgaaatc
     4141 gaacgcgacg gcaaaattgt cgaaacggtg tcgtccgggg gaattttcgg cgagatggct
     4201 ctgatcgatg gagcgccgcg cgccgccacg gcacgcgcca agacggcctg cgaggtcgcc
     4261 cctgtcagcg agaaaacctt cctgttcctc gtgcacgaga caccatattt cgcaatctcg
     4321 gtgatgcgca cgcttgccga ccgtctgcgg cgttcgcagc cgtaagactg gactgttacc
     4381 cactctttag cctcccggct tgccatcatg gctgacgcca gccatgatcg agtcccgtca
     4441 accccaagct gcgttaggcc cttgtctctg ctcgcgaatc tcgcgctccg ccgcccgcgc
     4501 aatatcgacg cgtctacgtt tgggcgggac gccatggccg gtgtcatctc cgccgtggtg
     4561 cagatcgcct attgcatctc gtttgccgcg ctcattttca ccggcgacct ggcaggcggc
     4621 tttccgctcg gccttgccgg gttgatcatg gggaccgtgg tcacctgcgt cgtgatcgcg
     4681 atcacctcga ccttctcccc cgtcatcggc ggaccggact cgccggccgt ggcggtgatg
     4741 agcgtgctcg ccgcctcgat cgccaccgca cttgcggcca agggcgccag caccgatcag
     4801 atcatcgtca atgtgctggt cgcgctctcg gtcagcacgc tgctcaccgg aatcctgctt
     4861 tgcggggtcg gcacgcttcg ccttgggcaa tggctccgct tcatcccgta tcccgtggtt
     4921 ggcggcttcc tcgccgcttc agggcttttg ctcatcaccg gcggcatgga agtcgtgacg
     4981 cagaccgatc tcacgctcct gccttcaagt tgggcgctgc tctattcccc gctctacgct
     5041 ccacagattg tcgtcgcaat gctgtttgcc gtcgccatcc ccgtgatggc gcgattcgtg
     5101 ccggcctacc ttgccctgcc gctggtgttc ttcgcttttc tcatcgtcct caacgccggg
     5161 ctgttcggcg ttgtgggtga cgatggcgtg cgcggcgcgt ggttcctgcc cgagcttggc
     5221 gagctcaccc tgtggtggcc catcaacgcc atgatcgggg accaaatcga ttggggcgtg
     5281 atcgcgcgga gcagcgccga gatcggctcc ttctgcggcg tcatggcgat tgcgcttctg
     5341 ctcgacgtat cgagcctcga agtggcgcgc cagaaatccg gcgacctcga ccaggagttt
     5401 cgctcgaacg gcctcgccaa cctgcttgcc tcggtgctcg gcggcttcgg cgggtcgttg
     5461 tcgatgaatg cctgtctctt gctcgatgag tccggcgcca ccacgcgctg ggcgggcgcc
     5521 attgtcggcc ttgtctgcgc gctgatcctg ttttccggcc tcgatgtcgg gagcatcgtg
     5581 ccgaaggcca ttctcgccgg gatgctcatc tatctcggcg ccgtgatcct gatcgagttg
     5641 tggaacgcgc ccgccgaaag ctcatggatg gaatgggggc tcaccgggat catgacgctc
     5701 gtcatcatca atctcggtta tttcatgggc gtggtcctgg gggtcatcgg cgcctgcctg
     5761 atgttcgcgc tgagctatag ccgcatcggc gtgatcaggc gtcacctcac gcgccaggag
     5821 ttctcgagca atgtcgagcg ctcgccggag cagtcgcgcc tcttgcgcga ggagggcaag
     5881 cgcgtccacg tcttctggct ctcgggcttc atcttcttcg ggtcgtccaa cggtctgttc
     5941 gagcgcatca agcgggtgat tgaggcgcag cgggagaaac ccgtcgcctt tgtggtgctc
     6001 gatttcggcg gggtgcccgg gctcgatact tcggcggtgc tgagcctcgt caagctccgc
     6061 aacttttgcg aggagcacga cgtcacgctc gggttctcgg gcttgagcga aagcatgaag
     6121 gcgagcttcg agcgagcggg gtttttcggc agcacccggg cgcatcaggt cttcgggagc
     6181 cgcaacgagg ccgtcgaatg gtgcgaggac atgctgctca tgcatcacga ggtcggcgag
     6241 atgtcgaccc acagcttcga gacgtggctc gaatccgaat tcggcggcac ggtcgatttc
     6301 gcgcgcatcg cgtccttcat ggagcgccag gagctgaaca atggcgcgtt cctgttccgg
     6361 caaggggagg cggccgattc gatcgtgctg caagcctcgg gcagcgtcgc catcaccatc
     6421 atcgacgagc acgggcgccc gatcaggctc cggcgcatgg tcgggcacac ggtggtgggc
     6481 gagatgggct tctatcgcgg cgtgccgcgc acggcgagcg tgatcgccga ggggccgacc
     6541 gtgatctacc ggctcacgcg caaggcgttc gaccgcatgc aagaagaaga cccggcggcc
     6601 gccgcggccc tgcacaagct catcatcagg ctcctgtcgg accggctcga attcgccaat
     6661 cgtgaaatct cggccctgct ttaaggagcc gtcgccagca atgccgcgac gatcgcgaga
     6721 taggtcagcc catggatcag ctgatcggtg ccgagcgcat gccagaagcc ggggtcctgc
     6781 gccgtcaggc cattacgctg aatgacctgc tccttcagcc agtcgatatg atagtggagc
     6841 aggaattcgc taacggcgat cgcgcccgct acgagaaggg agccgggaac cagcaccaga
     6901 tagacgagcg gggtcagcgc gacgtggatg ccggaatgga ggagaccgcc gggatggcca
     6961 tagattcctt tgttcagata ctgatacggg gtctgcaggt agaaatcggc gaccgcgtgc
     7021 ttgaccagga gcacggcgac ggcggcaagc gcgacgaggg cgaggtccgg catcggcgct
     7081 acttcgagag agcctcggcg gtcccgtccc attgcggcgg gggcgggttc acccggaaat
     7141 aggcgatgcg ctcgcggtaa agtgcgtaga gcttgtcgag gcgcgaggtg ttcagtttct
     7201 cgcattcgcg actgagggcc tcggcggcat cccactcttt cgcgcgatag cgggcgagga
     7261 acgcgccatg ccgctcggcg agcctgatga aatcctgcga ttgcttgaag gcgctgtcgc
     7321 cgaggagcgc gaagacccgc gtcgcctcgg tcttgccttt gaccttgagg aggtcgagct
     7381 cgagaaaggc gaaatccggc gctcgcacg
//
LOCUS       DASUQP010000128         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1559647, whole genome shotgun sequence.
ACCESSION   DASUQP010000128 DASUQP010000000
VERSION     DASUQP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559647"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /gene="nusA"
                     /locus_tag="VFY74_05800"
     CDS             <1..1293
                     /gene="nusA"
                     /locus_tag="VFY74_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX5931249.1"
                     /translation="IVQKVREAERERQYDEYKDRVGEIVNGIVKRVEYGNVIVDLGRA
                     EAIVRRDETLPRENFRYGDRIRAYVYDVRREQRGPQIFLSRTHPEFMAKLFAQEVPEV
                     YESIVTIKSVARDPGSRAKIAVVSRDASIDPVGACVGMRGSRVQAVVNELQGEKIDII
                     QWSQDAATFIVNALAPAEVVKVVLDEDAERIEVVVPDQQLSLAIGRKGQNVRLASQLT
                     GWDIDIMTEASESERRQAEFAERSQTFMEALDVDEVIAQLLASEGFSSVEEVAFVEPS
                     EIASIEGFDENTAAEIQTRAREHLEKIEAELEAKRKALGVADQLAEVPGITTAMLVML
                     GENNIKTVEDLADCATDDLAGWSERKDKETIRHGGILDSFGLSKDQVEDLILAARVKA
                     GWITEADLAPPPPPEGVEGTAESGEPGGAEASNGASPS"
     gene            1313..1987
                     /locus_tag="VFY74_05805"
     CDS             1313..1987
                     /locus_tag="VFY74_05805"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5931250.1"
                     /translation="MRQAAAERGRKSRDDADPTGRRCALSRAQRPRDELIRFVLAPDG
                     TVVPDLKERLPGRGMWLTASRDTVADAVKRKVFARALKSEAKVPEELPAQVERLLAEA
                     ALSALALANKAGEAAFGFAKVEEAIARGRVLALIHAREAAEDGCRKLDGKARGASFGR
                     TIPAIRAFGTDELSLATGRPNVIHAALIQGGAAERVLAAASRLERYRKGRAAFASQNE
                     VDTDKE"
     gene            1984..>3678
                     /gene="infB"
                     /locus_tag="VFY74_05810"
     CDS             1984..>3678
                     /gene="infB"
                     /locus_tag="VFY74_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5931251.1"
                     /translation="MSETNETEGKTARGARKMTLNVRRTVESGHVRQSFSHGRSKSVL
                     VEKKKRRAIGPGGAAEPVAEEAKPAAAARPAEKARPKAEEAQANRVRVVLPQLSEEEK
                     DARMRALTESRQREAAEREKVKETAALQVVTEEQRAKERREADERKQKEDERHEAEAK
                     ARKVGEETARKHLLKEEADEAARPADARHKKSAVAPRRGEERRVRGRLTIINALDEEQ
                     RQRSLASLKRRQERQKKQITGPQAQAKIQRDVVIPEVITIQELANRMAERGVDVIKYL
                     MKDGEMRKITDVIDADTAQLIAEEFGHSVRRVSEADVEEGFIGAEDQAEDRQPRAPVV
                     TIMGHVDHGKTSLLDAIRATNVVSQEAGGITQHIGAYQVETQAGQKITFIDTPGHEAF
                     TAMRARGAKVTDIVILVVAADDGVKPQTVEAINHAKVARVPLIVAINKIDKPGADPQR
                     VRTDLLSHEIVVESLGGDTLEIEVSALKKTGLDKLLEAVLLQAEVLELTANADRPAEG
                     VIVEAKLERGRGPVGTALIQRGTLKLGDLIVAGQAWGRVRALINDVGEQVKEAGPSA"
BASE COUNT          733 a         1167 c         1282 g          496 t
ORIGIN      
        1 atcgtgcaga aggtgcgcga ggccgagcgc gagcgccaat atgacgagta caaggaccgc
       61 gtcggcgaaa tcgtcaacgg catcgtgaag cgcgtcgagt acggcaatgt gatcgtcgat
      121 ctcggccgcg cagaggcgat cgtgcgccgc gacgagaccc tgccgcgcga gaatttccgc
      181 tatggcgatc gcatccgcgc ctatgtctac gacgtgcgcc gcgagcaaag gggcccgcag
      241 atcttcctct cgcgcaccca tccggagttc atggccaagc tcttcgccca agaggtgccc
      301 gaggtctatg agagcatcgt caccatcaaa tctgtggcgc gcgatccagg cagccgtgcc
      361 aagatcgccg tggtgtcgcg cgacgcctcg atcgatccgg tcggcgcctg cgtcggcatg
      421 cggggcagcc gcgtgcaggc cgtggtgaac gagcttcagg gcgagaagat cgacattatc
      481 cagtggtcgc aagatgccgc gactttcatc gtcaacgcgc tggcgccggc cgaagttgtc
      541 aaggtcgtgc tcgacgagga tgccgagcgc atcgaggtgg tcgtgcccga tcagcagctg
      601 tcgctggcca tcggccgcaa gggccagaac gtgcggctcg cctcccagct caccgggtgg
      661 gacatcgaca tcatgacgga ggcttcggaa tcggagcgtc gccaggcgga attcgccgag
      721 cgttcgcaga ccttcatgga ggcgctcgac gtcgacgagg tcatcgcgca gttgctggcc
      781 tccgaagggt tcagctcggt cgaggaagtc gccttcgtcg agccgtcgga aatcgcctcg
      841 atcgagggct tcgacgagaa cacggcggcc gagattcaga cgcgcgcgcg cgagcacctg
      901 gagaagatcg aggccgagct cgaagccaag cgcaaggcgc tcggcgtcgc cgaccagctt
      961 gccgaggtcc cggggatcac gaccgcgatg ctcgtcatgc ttggcgagaa caatatcaag
     1021 acggtcgagg acctcgccga ttgcgccacc gacgacctcg ccgggtggag cgagcgcaag
     1081 gacaaggaga ccattcgcca tgggggcatc ctcgacagct tcggtctgag caaggaccag
     1141 gtcgaggact tgatcctggc ggcgcgcgtc aaggcgggat ggatcacgga ggcggatctt
     1201 gcgcctcctc ctcctccgga gggtgtggag gggacagccg agagcggcga gcccggcggc
     1261 gctgaagcgt cgaacggcgc gagcccttca tgagcctgcg aggagaccat cgttgcggca
     1321 ggcggcggct gagcggggac gcaagtcccg agacgacgcg gacccgaccg gtcgccgctg
     1381 tgctctctcc cgcgcgcaga ggccaaggga cgagctgatc cgcttcgtcc tcgcacccga
     1441 cggcacggtc gtaccggatc tcaaggaaag gctgccgggc cgcggcatgt ggctgacggc
     1501 gtcgcgcgat acggtcgccg acgcggtcaa acgcaaggtc ttcgcccgcg ccctgaaaag
     1561 cgaggccaag gttcccgaag agctcccggc acaggtcgag cgtcttctcg ccgaggcggc
     1621 cttgtcggcg ttggcccttg ccaacaaggc cggggaggcg gcattcggct ttgccaaggt
     1681 ggaggaagcc atcgccaggg gtcgcgtttt ggccctgatc catgcccggg aagcggccga
     1741 ggatggttgc cgcaagctcg acggcaaggc ccgtggcgca agcttcgggc gcacgattcc
     1801 ggccattcgc gccttcggca cggacgaatt gagtttggca acgggccggc caaatgtgat
     1861 acatgctgcc ctcatccagg gcggagcggc agagagagtc ctcgcggcag cctcgcgctt
     1921 agagcgctac cgcaagggcc gcgccgcatt tgcaagtcag aacgaagtgg atacggacaa
     1981 ggaatgagtg aaacgaacga gaccgaaggc aagaccgccc ggggcgcgcg caagatgacg
     2041 cttaatgtgc gccggacggt cgagtccggt catgtccggc agagtttcag ccacggtcgc
     2101 tcgaaatccg ttctggtcga gaagaagaag cgccgtgcca tcggcccagg cggcgcggca
     2161 gagcccgtcg cagaggaggc gaaaccggcg gccgccgccc ggccggccga aaaggcaagg
     2221 ccgaaggccg aagaggcaca agcgaaccga gttcgcgtcg tgctgccgca actgtccgag
     2281 gaagaaaaag acgcgcgcat gcgcgctctg accgaatcgc gccaacgcga ggcggccgag
     2341 cgcgaaaagg tcaaggagac cgcggccctt caggtcgtca cggaagagca gcgggcgaaa
     2401 gagcgcagag aagccgacga gcgcaagcaa aaagaggatg aacggcacga ggccgaggcc
     2461 aaggcgcgca aggtcggcga agagaccgcg cgcaagcatc ttctcaagga ggaggccgat
     2521 gaggccgcgc gcccggccga cgcgcgtcac aagaagagcg cggttgcccc gcggcgcggc
     2581 gaagagcgca gggtcagggg ccggctcacc atcatcaacg cgctcgacga ggagcagcgg
     2641 cagcgcagcc tcgcctcgct gaaacgccgc caggagcgtc agaagaagca gatcaccggc
     2701 ccgcaggcgc aggcgaagat ccagcgcgac gtcgtgatcc ccgaggtcat caccattcag
     2761 gagctcgcca accgcatggc cgagcggggc gtcgacgtca tcaagtacct gatgaaagac
     2821 ggcgaaatgc gcaagatcac cgatgtgatc gacgccgaca ccgcgcagct catcgccgag
     2881 gagttcggcc atagcgtgcg gcgcgtctcg gaggccgacg tcgaagaggg cttcatcggc
     2941 gcggaagatc aagccgaaga tcgccagccg cgcgcgcccg tggtcaccat catgggccat
     3001 gtcgaccacg gcaagacgtc actgctcgac gccatccgcg cgaccaacgt cgtctcgcag
     3061 gaggccggcg gcatcaccca gcatatcggc gcctaccagg tcgagactca ggccgggcag
     3121 aagattacct tcatcgatac gccgggccac gaggccttca ccgccatgcg cgcccgcggc
     3181 gccaaggtga ccgatatcgt gatcctggtg gtcgccgccg atgacggggt gaagccgcag
     3241 acggtcgagg cgatcaatca cgcgaaagtc gcccgcgttc ccctgatcgt cgccatcaac
     3301 aagatcgaca agccgggcgc ggatccgcag agggttcgca ccgacctctt gagccacgag
     3361 atcgtggtcg agagccttgg cggggatacg ctcgagatcg aagtctcggc gctcaagaag
     3421 acggggctcg acaagctgct tgaggcggtc ctgctccagg ccgaggtgct cgagctcacc
     3481 gccaatgccg atcgtcccgc cgaaggcgtg atcgtcgagg ccaagctcga gcgcggccga
     3541 ggcccggtcg gcacggcgct gatccagcgc ggcacgctca agctcggcga cctcatcgtc
     3601 gcgggccagg cctggggccg cgtgcgggcg ctgatcaacg atgtcggcga gcaggtgaag
     3661 gaggccggcc cctcggcc
//
LOCUS       DASUQP010000129        15424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1970729, whole genome shotgun sequence.
ACCESSION   DASUQP010000129 DASUQP010000000
VERSION     DASUQP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15424
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970729"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFY74_05815"
     CDS             <1..256
                     /locus_tag="VFY74_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931252.1"
                     /translation="KMKSKSGVRVEQWPEEVLSALRSAWGEIAKEESDQDYFFRIVLE
                     DIAKFRGAAQKKSAPAPSAAAPQAAPEPRPDAAAKTKPAP"
     gene            complement(266..727)
                     /locus_tag="VFY74_05820"
     CDS             complement(266..727)
                     /locus_tag="VFY74_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX5931253.1"
                     /translation="MAAEELLYNRPQGHKQGTILRRSEMSRFQFVGVAAVSFFMLSGT
                     ALAQGDAAKGKEAYAKCGICHQVGPTAQNTVGPELNKIVGAKAASKPGFAYSAGMKKL
                     GEEGFVWTPENIDKWIADPKAMLPDSPMSQLFPGMPDAQERANIIAYLQTQ"
     gene            929..2365
                     /locus_tag="VFY74_05825"
     CDS             929..2365
                     /locus_tag="VFY74_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5931254.1"
                     /translation="MSNIAMPSPDHAILQRRNELLKQLAKLVPEAALITDREGRRTFE
                     TDALTAYRCLPLAVVLPGSTEEVSAILRFCHYNHIKVVPRGAGTSLCGGALPLEDAIV
                     LCLSRMSKVLDIDLENRAARVEAGMTNSGITDAVAGDGFFYAPDPSSQIACTIGGNVA
                     TNSGGAHCLKYGVTTNNVLGVRMVLMNGEVIDIGGTSLDAPGYDFLALIVGSEGQFGV
                     VTEVTVRLLKAAEGARPMLIGFGSNESAGACVAAIIAAGIVPVALEFMDRPAIHVCEH
                     FVAAGYPLDVEALLIVEVQGSEDEIDTLLERIGEIAQDFDPITIRRSQSAEESARIWK
                     GRKAAFGAIGQLSDYYCMDGVIPLSKLPKALDEVGWICRRYRFDVANIFHAGDGNLHP
                     LILYNANDPLQLERVELCGAEILKLCVELGGCLTGEHGVGIEKRDLMRVQFSDADLAQ
                     QLRIKAAFDPDWLLNPGKVFPLDQRGTQ"
     gene            2362..3597
                     /gene="glcE"
                     /locus_tag="VFY74_05830"
     CDS             2362..3597
                     /gene="glcE"
                     /locus_tag="VFY74_05830"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX5931255.1"
                     /translation="MTGHMRVGFHAPGTVEELAYLIAEAADTRTPIEVMGRGTKREVG
                     RPTQHGAVLSTENLVGVPLYEPSELVLVVRAGTPLAQIEQLLAENEQELPFEPVDLGH
                     MLGFGPGQGTIGAVIATNLSGSRRIHSGSARDHVLGIQAVNGQGETIRSGGRVMKNVT
                     GYDLARGLAGSWGTLAVMTEIALKVLPAQREMRTVLCFGLADPTAVQVLSQALGTPYE
                     VSGTVHIHAGLVERFSDQEIANAGASVTAIRVENFPASARYRSSRLKQMLQAYAPAHE
                     LDTMRSRVFWNEVRAMRMFQKSSRPLWRISTPPSTAAKLVRAVTRKIDVRVFYDWSGG
                     LIWLETPPISDAGAVEIRRTLAEFGGHATLIRAEAQARAVIDVFQPLDPPLMALTQSL
                     KRAFDPVGILNPGRMYAGI"
     gene            3607..4968
                     /gene="glcF"
                     /locus_tag="VFY74_05835"
     CDS             3607..4968
                     /gene="glcF"
                     /locus_tag="VFY74_05835"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX5931256.1"
                     /translation="MQTSFNEVQLADPTIARADAILKRCVHCGLCIATCPTYVLTGDE
                     RDSPRGRIYLMKQMFEERQVTASMTHHIDRCLSCFSCMTTCPSGVDYMHLVDLARVRI
                     EAKGQRSPREQGLRWILAKVLPNPRRFRLALMLGWLARPFRGLMVRFGLEGPASALAL
                     VPSRLPETKLARPRSEIGTNPKAKRVALMLGCVQEVLAPAISRAAIRLLRRHGVDVVV
                     VKDETCCGALVHHLGKEEEARGYARRNIDAWTAVIREKPLDAIVVTTSGCGTMVKDYG
                     NLLARDRGYAERAAYVAGLARDITEFIDEIGLMPPMMWTGLRIAYHAACSLQHGQKLD
                     ALPLSLLEQAGFSILPIPEGHLCCGSAGTYNLLEPELSGQLRARKLANIAKVDPDVIV
                     TANIGCMMQLKGGTSAPFVHTVEMLDWATGGPAPEALGKLKDAGHPIESLVELAKQTA
                     LVD"
     gene            5219..6694
                     /gene="hisS"
                     /locus_tag="VFY74_05840"
     CDS             5219..6694
                     /gene="hisS"
                     /locus_tag="VFY74_05840"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5931257.1"
                     /translation="MSKKTEPARPKTRLPKGLSDISASEIRATEEMLGTIRRVFESYG
                     FEALDTPAIEYSDALGKFLPDQDRPNEGVFSFKDDDEEWLSLRYDLTAPLARYVAENY
                     QRLPKPFRRYQVGPVWRNEKPGPGRFRQFTQFDADTVGASSVAADAELCMLAADTLEA
                     LGVSRGQYVVKVNNRKVLDGVLEACNVKAAPQRLAVLRAIDKLDRLGLDGVKALLGEG
                     RKDESGDVTKGAGLKPTQIEPVLNMIDPDQDEEDRIASMDFSVGKSASGQAGVAETEE
                     IKRLIAATGYSDRVKVDRTVVRGLEYYTGPVFEAELLAPIKDERGVPIRLGSVVSGGR
                     YDGLIERFTGEKVPATGVSIGVSRLRFGLQRLGKYGESAARGPVIVLVLDRDGVADYQ
                     NMVQRLRSAGIRAELYLGDGGLKAQMKYADRRGAPCVVIQGGDEKAKGAIQVKDLILG
                     ATLTGIEDRELYLKQQAEAQALVPEAKLVETVRAVLARHKA"
     gene            6698..7867
                     /locus_tag="VFY74_05845"
     CDS             6698..7867
                     /locus_tag="VFY74_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5931258.1"
                     /translation="MAAESAKEFEALEAQAATLLDTFRNAGYEQVAPAILQPADVFLD
                     RIGEQIRSRTYVFTDLAGDELCLRPDLTVPVSRLYLERHPEARAEARYCYNGPAFRFQ
                     PQGDNRARPREFRQAGIECFGDLDKESADVEIVLLAVEAVRRAGLKDFELRFGDIAMF
                     YALLDALALPERWRLKLMHYFWRPASFHALLSRLALGERPNGDAPVIAFAGALAGATQ
                     DRAEDRVAAYLEEQGLPLAGNRTLGEIAARVLDQAADSSAGPLRKEVAAVIDSYLAIS
                     GVPKTAAARVAALAKDAGIDLSPALASAARRFERLERSGIDLARATFATEFGRDLEYY
                     SGLVFQIESPGLKDAIAGGGRYDGLLADLGAPREVPAIGSAIHTERLLAAVRGTS"
     gene            7867..8559
                     /gene="hisG"
                     /locus_tag="VFY74_05850"
                     /pseudo
     CDS             7867..8559
                     /gene="hisG"
                     /locus_tag="VFY74_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
     gene            8767..9522
                     /locus_tag="VFY74_05855"
     CDS             8767..9522
                     /locus_tag="VFY74_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5931259.1"
                     /translation="MLIRLALALVLAVSASSALAAPSRIIILRHGETMDPWNLCEIGA
                     QRAKALALTYLGRDALKSLFTDGEEPAFFFAVTPNTAATASPSVAGWNKPVILYSVVP
                     QAGQSEDDFTAALSRRTQEAARNVLANPTLQGRTVVMVWERAHIASEALEAKHENEAV
                     TLRQLLKLDILPGVPKTWPADNHDYFWIVDFPPNSNVPSKFEMAKQEFGASFPRVPAN
                     DWDTPNGLDAASGCKPESKTERNPRRREDRPAF"
     gene            complement(9491..11263)
                     /locus_tag="VFY74_05860"
     CDS             complement(9491..11263)
                     /locus_tag="VFY74_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX5931260.1"
                     /translation="MANLKKTFKDFAGIAIPYYQSEDRWAGRVLLSAVIGLQLFQVWL
                     NVQFNAWYNTFYTSLQNKDWDTFIWQIGVFSLLAAFFIVSAVYQLYLQQWLQIRWRNW
                     LTQRYLGRWLGQGTHYRMRLKGDQADNPDQRIADDIREFVDGTLTIGIALLGSIVTLV
                     SFIVILWNLSATTPLMIGSKAFEIPGYLVWAALIYAILGTWVTHLVGRPLIKLDFDQQ
                     RYEADFRFSLVRLRENAEEVTLLAGEEAEEARLRDRFGSVIRNWYEIMRRRKRLTFLT
                     AGYSQVAIIFPFIVVSPVYFFGSMTLGGLMQIASAFGQVQGALSFFVTAYTAIANWKA
                     VLNRLTGFEASIAWAQSLDRTAPRLEFLSDGGNALIVEELAVRLPDGQEIVRLAEFSI
                     EPGERVLVTGASGAGKTTLFRTLGGVWPFGSGSIRVPQGASVLVLPQRSYMPLGTLRG
                     ALAYPGAPDAFTPDEIEEVLKAVGLESFSSELDETAYWADKLSGGEQQRLSIARALLQ
                     KPQWLFLDEATGALDEASEAALYRLLIERLPGTAIVSIGHRASLAQFHQRFFELKPDE
                     TGRHHLREIKGKPKAKKPVDLPVA"
     gene            complement(11755..13392)
                     /gene="groL"
                     /locus_tag="VFY74_05865"
     CDS             complement(11755..13392)
                     /gene="groL"
                     /locus_tag="VFY74_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401309.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5931261.1"
                     /translation="MAAKDVKFSAEARERMMRGVDILSNAVKITLGPKGRNVILEKSF
                     GAPRSTKDGVTVAKEIELEDKFENMGAQMLREVASKTNDTAGDGTTTATVLAQAIVRE
                     GLKSVAAGMNPMDLKRGIDLAVAEVVKDIARQAKKVKSSDEIAQVGTVAANGEKAIGD
                     MIAEAMQKVGNEGVITVEEAKSLDSELEVVEGMQFDRGYISPYFITNAEKMMAELESP
                     YILIYEKKLSGLQALLPLLEAVVQSGHPLLVIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLQDIAVLTGGELISEDLGIKLENVTVNMLGKAKKVVITKDDTTIVD
                     GAGKKPDIQARIAQIKQQIEDTTSDYDKEKLQERLAKLAGGVAVIKVGGATEVEVKEK
                     KDRVDDALNATRAAVEEGIVPGGGVSLLWAAKALKTKGDNQDQDAGINIVRRALQAPI
                     RQIAENAGVEGSIVVGKVTDQKSASFGYDAQNNEYGDLISKGIIDPAKVVRTALQDAA
                     SVAGLLVTTEAGVAESPKKKDEHSHMPGGGMGGMDMM"
     gene            complement(13445..13732)
                     /locus_tag="VFY74_05870"
     CDS             complement(13445..13732)
                     /locus_tag="VFY74_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5931262.1"
                     /translation="MKFKPLHDRVVVRRLEEEQKTKGGIIIPDTAAEKPQTGEVIAAG
                     PGARGEDGKLQPMGVKAGDKVLFGKWSGSEVKIDGEELLIMKESDIMGILG"
     gene            complement(13879..14868)
                     /locus_tag="VFY74_05875"
     CDS             complement(13879..14868)
                     /locus_tag="VFY74_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HEX5931263.1"
                     /translation="MKASFVALGGAAALLGLHLFAAAETVRAADPTPADLVDALNGVF
                     GKPQGTRSAHAKGFCLTGQFTPAPDAAKLSKAAHFAKPVPITARFSLGGGNANAPDNV
                     KGNVRGLAIRFDLGGGAFTDLVMISAPVFFVTTPALFVEFLQTVGSGDKAKLDAFFAA
                     HPESTRQAAWLNSHPVPASYAGVGYFGVHAFTFTNADGEKALVKYKVVPDAGELGLSD
                     EEAKSKGDNFYEEEIKERLGKGPVSFELQAVRGRDGDQTSDPTQRWEDEDGRETTPLG
                     KISIEALAPASACDAFSFLPGNVTDGIAGPADDPIFQIRSADYIVSFGRRRAQ"
     gene            15097..>15424
                     /locus_tag="VFY74_05880"
     CDS             15097..>15424
                     /locus_tag="VFY74_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931264.1"
                     /translation="MARTTAMATGRSEISGGLAMLDVIVLIAMAVTAAAFAVGVILHS
                     GIAQIPALIAAAALYMVMAASYLMVARSRPSGGSGARLGDFEEALEIIDRDLQRIDRL
                     EEEISRL"
BASE COUNT         2725 a         5044 c         4851 g         2804 t
ORIGIN      
        1 caagatgaag tcgaagagcg gcgttcgcgt cgagcagtgg ccggaggagg tgctttcggc
       61 gcttcgctcg gcttggggcg agatcgccaa ggaggagagc gaccaggact atttcttcag
      121 gatcgttctg gaggacatcg ccaagttccg cggcgcagcg cagaagaaat ctgcgccggc
      181 gccgtcggcc gccgcgccac aggccgcgcc ggagcctcgg cccgatgcgg cggccaaaac
      241 gaaacccgcc ccttaagccg cgtgcctatt gcgtctgaag ataggcgatg atgttggccc
      301 gctcctgagc gtcaggcatt cctgggaaga gctgagacat gggcgaatcc ggaagcatcg
      361 cctttggatc ggcgatccat ttgtcgatat tctccggggt ccaaacgaaa ccctcttcgc
      421 cgagcttctt catgcccgcg gaataggcaa aaccgggttt gctcgcggcc tttgccccga
      481 cgatcttgtt gagctccggc cccaccgtgt tctgggcggt cgggccgacc tgatgacaga
      541 tgccgcactt cgcgtaggcc tctttgcctt tggctgcgtc gccctgggcc aacgcggttc
      601 cactcaacat aaagaaagat acagcagcaa ctccaacaaa ctggaaacgg ctcatctcgc
      661 ttctccttag gatcgtgcct tgtttatgtc cttgcggacg attgtagagc aactcttccg
      721 cggccaattc atctagaacc ggcgaaatgt agcagcaaga gcgaaatccc tcaaaacggg
      781 cgagcggtca tccctaacgc attgaggagg aagggcggtt ggtatttccg gccggctccc
      841 ctgggcgtat tgcccgcaag cccttaagca tgcgaaacac acggatcggc gaaccttcct
      901 tgagagacaa aagatcggac ctgctcggat gtcgaatatc gccatgccca gcccggatca
      961 tgcgatcctc cagcgccgca acgagctgtt gaagcagctc gcgaagctcg tgcccgaagc
     1021 cgcgctgatc accgaccggg aagggcgccg cacattcgag accgatgccc tgaccgccta
     1081 tcgctgcctg ccgcttgcgg tcgtgctgcc gggcagcacc gaagaggtga gcgcgatcct
     1141 gcgcttctgc cactacaatc atatcaaagt cgtgccgcgg ggcgccggca catctctctg
     1201 cggcggcgcc ttgccgctcg aggacgcaat cgtgctttgc ctgtcgcgca tgagcaaggt
     1261 gctggacatc gatcttgaga accgcgcggc gcgcgtcgag gccggcatga ccaattccgg
     1321 cattaccgat gcggtcgcgg gcgacggctt cttttacgcg ccggacccgt cgagccagat
     1381 cgcctgcacc atcggcggca acgtcgcgac caattcgggg ggcgcccatt gcctgaaata
     1441 cggcgtcacc accaacaacg tgctcggcgt ccgcatggtc ttgatgaacg gcgaggtcat
     1501 cgatatcggc ggcacgagcc tcgacgcgcc cggctacgac tttctcgctt tgatcgtcgg
     1561 ctctgaaggt cagttcggcg tcgtcaccga ggtcacggtg cggctgctga aggccgccga
     1621 aggggcccgc ccgatgctga tcggtttcgg ctccaacgag tcggccggag cctgcgtcgc
     1681 cgcgatcatc gcggcaggca tcgttccggt ggcgctcgag ttcatggacc ggcccgcgat
     1741 ccatgtgtgc gagcatttcg tcgccgcggg ctatccgctc gacgtcgagg ccctcctcat
     1801 cgtcgaggtg caaggctccg aggacgagat cgacacgctt ctcgaaagga tcggcgagat
     1861 cgcgcaggac ttcgatccga tcacgatccg ccggagccaa agcgccgagg aaagcgccag
     1921 gatctggaag ggacgcaagg cggcgttcgg agccatcggc cagctctccg actattactg
     1981 catggacggg gtgatcccgc tgtcgaagct ccccaaagcg ctcgacgagg tcggctggat
     2041 ctgccggcgc taccgcttcg acgtggccaa tatctttcat gccggcgacg gcaatctgca
     2101 cccgctgatc ctctacaacg ccaacgatcc gctccagctc gagcgcgtcg agctttgcgg
     2161 ggccgagatc ttgaagttgt gcgttgagct cggaggctgt ctcaccggcg agcatggcgt
     2221 gggcatcgag aagcgcgatc tgatgcgggt gcaattttcc gacgcggact tggctcagca
     2281 attgcgcatc aaggccgcct tcgacccgga ttggttgctc aatcccggca aggtgtttcc
     2341 gctcgaccaa cgaggtaccc agtgaccggg catatgcgtg tcggtttcca cgctcccggc
     2401 acggtcgagg agctggccta tctcatcgcg gaggccgccg acacgcgaac gcccatcgag
     2461 gtgatggggc gcggcaccaa gcgcgaggtc ggccgtccga cccagcacgg cgcggtgctg
     2521 agcacggaga atctggtcgg cgtcccgctc tacgagccga gcgagcttgt tctcgttgtc
     2581 cgggccggga cgccgctcgc tcaaatcgaa cagctcctcg ccgaaaacga acaggagctc
     2641 cccttcgagc cggtcgatct cggccatatg cttggcttcg gtcccggaca gggcacgatc
     2701 ggggccgtca tcgccaccaa tctctccggc tccaggcgca tccattcggg ctcagcgcgc
     2761 gaccatgtgc tcggcatcca ggccgtcaac ggacaggggg agacgatcag atccggcggg
     2821 cgggtgatga agaatgtcac cggctatgat ctcgcccggg gccttgccgg ctcgtggggc
     2881 acgcttgccg tgatgaccga gattgcgctc aaagtgcttc cggcccagcg cgaaatgcgc
     2941 accgtcctct gtttcggtct tgccgatccg acggccgtcc aggttttgag ccaggccctt
     3001 ggcaccccct acgaggtctc cggcaccgtg catattcatg ccggactcgt agagcgcttc
     3061 tccgatcagg aaatcgccaa tgccggcgct tccgtcacgg ccatcagggt cgagaatttt
     3121 ccggcctccg cccgctaccg ctccagccgc ttgaagcaga tgctccaggc ctacgcgccc
     3181 gcgcatgagc tcgacaccat gcggagccgc gtcttctgga acgaagtcag agctatgcgg
     3241 atgttccaga aatcgtcgcg cccgctgtgg cgcatctcca cgccgccttc gaccgcagcg
     3301 aagcttgtca gagcggtgac gcgcaagatc gacgtgcggg tcttctacga ttggtccggc
     3361 ggcctcatct ggctcgaaac gccgcccatc tccgatgccg gcgcggtcga gatccgccgc
     3421 acccttgccg aattcggcgg tcatgccaca ctgatccggg ccgaggcgca ggccagggcc
     3481 gtgatcgacg tgttccagcc gctcgatccc ccgctcatgg cgctgaccca atcgctcaag
     3541 cgggctttcg atccagtggg aatcctcaat cccggccgca tgtacgcggg aatctaggcc
     3601 tgaaccatgc aaaccagttt caacgaggtc cagctcgccg atccgaccat cgcccgcgcc
     3661 gatgcgatcc tgaagcgctg cgttcattgc ggtctctgca tcgcgacctg cccgacctat
     3721 gtgctcacag gcgacgagcg cgacagcccg cgcggccgca tctacctgat gaagcagatg
     3781 ttcgaggagc gccaggtaac ggcgtcgatg acccatcaca tcgaccgctg cctctcctgc
     3841 ttctcgtgca tgaccacctg tccgagcggc gtcgactaca tgcatctcgt ggatctcgcc
     3901 cgggtgcgga tcgaggcgaa gggccaacgg tcgccgcgcg agcagggctt gcgctggatc
     3961 ttggccaagg tgctgcccaa tccgcgccgc ttccggctcg cgctgatgct gggctggctc
     4021 gcccggccgt tccgcgggct tatggtgcgc tttggactcg agggtccggc gtccgcgctt
     4081 gcgctcgtgc cgtcgcgcct gccggagacg aagctcgcgc ggccgcgctc ggagatcggc
     4141 acgaacccca aggccaagcg cgtcgcgctg atgctcggtt gcgtgcagga ggtgctggcc
     4201 cccgcgatca gccgcgccgc catcaggctg ctcaggcgcc acggcgtcga cgtcgtcgtg
     4261 gtcaaggacg agacttgctg cggcgcgctc gttcatcatc tcggcaagga agaggaggcg
     4321 cgcggctatg cccgccgcaa catcgacgcc tggaccgccg tcatccgcga gaagccgctc
     4381 gacgccatcg tcgtcaccac ctcgggctgc ggcacgatgg tgaaggacta cggcaatctg
     4441 cttgcccgcg accggggcta tgccgagcgc gcggcctatg tcgccgggct cgcccgcgac
     4501 atcaccgagt tcatcgacga gatcggattg atgccgccga tgatgtggac gggcctcagg
     4561 atcgcctatc acgccgcctg ctcgctccag cacgggcaga agctcgatgc cttgccgctt
     4621 tcgctgctcg agcaggccgg tttcagcatc ctgccgattc cggagggcca tctctgctgc
     4681 ggctcggccg ggacctacaa tttgctggag cccgagctct cgggccagct gcgcgcccgc
     4741 aagctcgcca acatcgccaa ggtcgatccg gacgtgatcg tcaccgccaa tatcggctgc
     4801 atgatgcagc tgaagggggg cacgtccgct cccttcgtcc acaccgtgga gatgctcgac
     4861 tgggcgacgg gcggccctgc gccggaggcg ctcggcaagc tgaaggatgc cggccatccg
     4921 atcgagtccc tggtcgaact cgccaagcag accgcgctcg tcgattgaaa cagcaaattt
     4981 tgactgggct taccccccca cccggcgcgc tatgcgcgcc gacctcccca cgagggggag
     5041 gttacgacac ctgatacctg atatctgata tctgatatct gatttctgat acctgacacc
     5101 tgacacctga ttaagacaca cgatacctga tatctgatat ctgatttccg acatctgatt
     5161 tctgacacct gatatctgat atctgacacc tgacaccctg acacccgaca cctgacaaat
     5221 gagcaagaag accgagcccg cgcggccaaa gacccgcctg ccgaagggcc tttccgacat
     5281 atccgcgagc gagatccgcg ccaccgagga gatgctcggg acgatcaggc gcgtgttcga
     5341 gtcctacggc ttcgaggcgc tcgacacgcc tgcgatcgaa tattccgacg cgctcggcaa
     5401 gttccttccc gatcaggacc ggccgaacga gggcgtgttc tcgttcaagg acgacgacga
     5461 agagtggctg tccctccgct acgatctgac cgcgccgctc gcccgctacg ttgcggagaa
     5521 ctatcagcgc ctgccgaagc cattccgccg ctatcaggtg gggccggtat ggcggaacga
     5581 gaagccgggc cccggacgct tccgccagtt cacccagttc gacgccgaca cggtcggcgc
     5641 gtcctcggtc gcggcggacg ccgagctctg catgctcgcc gccgacacac tcgaagcgct
     5701 cggcgtttcg cgcgggcaat acgtggtcaa ggtcaacaac cgcaaagttt tggacggcgt
     5761 cttggaggct tgcaacgtga aagcagcgcc gcagcgcctc gccgtccttc gcgccatcga
     5821 caagctcgac cgcctcggcc ttgatggcgt aaaggccctc ttgggcgaag gccgcaagga
     5881 cgagtccggc gacgtcacca aaggcgctgg attaaagccg acgcaaattg agccggtcct
     5941 caacatgatt gaccctgatc aagacgagga agatcggatt gcttcgatgg attttagcgt
     6001 cggcaaatcc gcgagtgggc aagccggcgt cgcggagacg gaagagatca agagattgat
     6061 cgcggccacg ggctattccg acagggtcaa ggttgaccgc acggtcgtgc gcggattgga
     6121 gtactacacc ggacccgtgt tcgaggccga actactcgcg cccatcaagg acgagcgcgg
     6181 ggtacccatc aggctcggct cggttgtgag cggcgggcgc tatgacgggc tgatcgagcg
     6241 cttcaccggc gagaaggtcc ctgcgaccgg cgtctccatc ggcgtatcgc gcctccggtt
     6301 cggcctccag cggctcggca agtatggaga aagcgcggcg cgaggaccgg tcatcgtgct
     6361 cgtgctcgac cgcgacggtg tcgccgacta tcaaaacatg gtccaacgac tgcgaagcgc
     6421 cggcattcgt gccgagctct atctcggcga cggcggcttg aaggcgcaga tgaaatacgc
     6481 ggaccgccgc ggagcccctt gcgtcgtcat ccaaggaggc gacgagaagg ccaagggcgc
     6541 cattcaggtc aaggatctca tcctcggcgc cacactcacg ggaatcgagg atcgggagct
     6601 atatctcaag cagcaggccg aagctcaagc tctcgtgccg gaagcaaaac tcgtcgagac
     6661 cgttcgggcc gtgctcgccc gtcacaaggc ttgagccatg gccgccgaat cggcgaaaga
     6721 attcgaggct ctcgaagccc aggccgcgac cctgctcgac acgttccgaa acgcaggtta
     6781 cgagcaggtt gcgcccgcga tcctccagcc ggcggacgtc tttctcgaca ggatcggcga
     6841 gcagatcagg agccgcacct atgtcttcac cgacctcgcg ggcgatgagc tttgcctccg
     6901 gcccgacctg acggtgccgg tgtcgcgcct ctatctcgag cgtcaccccg aagcccgcgc
     6961 ggaggcccgc tattgctaca acggccccgc cttccgcttt cagccgcaag gagacaatcg
     7021 ggcgcggccg cgcgagttcc gccaggcggg catcgaatgc ttcggcgatc tcgacaagga
     7081 gagcgccgat gtcgagatcg tgctgcttgc ggtcgaggcg gtgcgccgcg ccgggctgaa
     7141 ggacttcgag ctccgcttcg gtgacatcgc catgttctat gcgctgctcg atgcgttggc
     7201 gcttccggag cgctggcggc tgaagctcat gcactatttc tggcgtcccg cgtccttcca
     7261 cgcgctgctg tcgcgtcttg ccctcggcga gcggccgaac ggcgatgcac ccgtcattgc
     7321 gttcgccggc gcgcttgccg gcgcgaccca agaccgtgcc gaggatcgcg tcgccgccta
     7381 cctcgaggag caaggacttc cacttgcggg caaccgcacg cttggcgaga tcgcggcgag
     7441 ggtgctcgat caggccgccg attcgagcgc gggacctttg cgcaaagagg tcgccgccgt
     7501 gatcgactct taccttgcca tctcgggggt gcccaagacg gcagcggcgc gcgtggccgc
     7561 cctcgccaag gacgccggca tcgatctttc acctgcgctc gcgtccgccg cgcgccgctt
     7621 cgagcgcctc gagcggagcg gcatcgatct tgcgcgcgcg accttcgcca ccgagttcgg
     7681 acgggacctc gaatactatt cgggcctcgt gttccagatc gaatcccccg gcctcaagga
     7741 cgccatcgcc ggcggcggcc gttacgacgg cctgctcgcc gatctcgggg cgccgcgcga
     7801 ggtaccggca atcggctctg ccatccacac cgagcgcctg cttgccgcgg tgaggggcac
     7861 ctcctgatgg cagcgaagct cctgatcggt atcccctcga aggggcggct caaggatgcc
     7921 gcggccgatc tcttcgcgcg cgccggtctt tccttgcgca acaagggaca cgagcggcgc
     7981 taccgcggcg gcatctccgg cctcgacgat gtcgaagtcg tctacgcctc ggcggccgag
     8041 atcgtcgacc agctcaggac ggggcacgtc catctgggcg tgaccggcga ggatctggtc
     8101 cgcgagacga tcggcgatgt cgacggcgcg gtcgcgttcc ttaagccgct tggcttcggc
     8161 cacgcggacg tggttgtggc cgttcccgat tgctggatcg acgtcgtccg catggccgac
     8221 ctcgaagacg tggccgcgca atttgccggc gtgcacggca ggcgcctgcg tgtcgcgacc
     8281 aaatatatga atctgacgcg ccgcttcttc gcaagccacg gcgtcgccgg ctatcgcatc
     8341 gtcgagagcg ccggcgccac cgaagccacg ccagcggcgg ggaccgccga gctgatcgtc
     8401 gatatcacca ccaccggcac gacgctccgc gccaatcacc tgaaggtgct tgaggacgga
     8461 ttgatcttga ggtcgcaggc gcatctcttc gcctcgcgcg ccgccgcctg ggataggggt
     8521 gccgagaaga cccgcgccgc cattgttgag cgattgtcgc gaccgtcatc gtcatcctga
     8581 gggttcgact gaagcgagcc tcgaaggatg attgaagagt actgcgtcct tcgagggccg
     8641 cttcgcggcc tcctcaggat gacggaggtt ggagaagcgg cggtccgtcg agggccgctt
     8701 cgcggcctcg gtatgacggt gaattgaatg gcggcgctga gaaccggccg gtagtctccg
     8761 ccgcccatgt tgatccgcct cgccctcgcg ctcgtcctcg cggtctcggc ctcctccgcg
     8821 cttgccgcgc cttcgcgcat catcatcttg cgccacggcg agacgatgga cccttggaat
     8881 ctgtgcgaga tcggcgcgca aagggccaag gcgctcgcct tgacctatct tggccgcgac
     8941 gccctcaagt ctctgttcac ggacggcgag gagcccgcct tcttcttcgc cgtcacgccc
     9001 aacacggccg caaccgcctc gccttcggtc gcgggctgga acaagccggt catcctctat
     9061 tcggtcgtgc cgcaggcggg acagagcgag gacgacttca ccgcagcgct cagccgccgc
     9121 acgcaagaag cggcccgcaa cgtgctcgcc aatccgacgc tccaaggcag gacggtggtc
     9181 atggtgtggg agcgcgcgca catcgcaagc gaggcgctcg aggccaagca cgagaacgag
     9241 gcggtgacgc tccgccaatt gctcaagctc gacatcctgc cgggcgtgcc gaagacttgg
     9301 cctgccgaca atcacgatta cttctggatc gtggacttcc cgccgaactc caacgtgccc
     9361 tccaagttcg aaatggcgaa gcaggagttc ggcgcgtcct tcccgcgcgt gccggcgaac
     9421 gactgggaca cgcctaacgg gctcgacgcg gcaagcggct gcaagcctga atcaaagact
     9481 gagaggaacc ctaggcgacg ggaagatcga ccggcttttt agcttttggc ttgcctttga
     9541 tttcgcgcaa atgatgccgg ccagtctcgt cgggtttcaa ctcgaagaag cgctggtgga
     9601 attgcgcaag gctcgcgcga tggccgatcg acacgatggc cgtgccgggg agccgctcga
     9661 tcaagaggcg atagagcgcc gcctcggacg cctcgtcgag cgcgccggtc gcctcgtcga
     9721 ggaagagcca ttgcggcttc tgcaggagcg cacgcgcaat cgagagccgc tgctgctcgc
     9781 cgcccgaaag cttgtcggcc cagtaggcgg tctcgtcgag ctccgagctg aaggactcaa
     9841 gtccgaccgc cttcagcacc tcttcgatct cgtccggcgt gaaggcatcc ggcgctccag
     9901 gataggcgag ggcccccctc aacgtgccga gcggcatgta ggaccgctgc ggcaggacaa
     9961 gcacgcttgc gccttgcggc acgcggatcg agcccgagcc gaacggccac accccgccga
    10021 gcgtcctgaa cagggttgtc ttgccggcgc ctgacgctcc cgtcaccagc acgcgctcgc
    10081 ccggctcgat cgagaactcg gcgagccgca cgatctcctg cccgtcggga agcctgacgg
    10141 cgagttcctc gacgatgaga gcgttcccac cgtcactgag gaattccagc ctcggagcgg
    10201 tgcgatcaag gctctgcgcc catgcgatcg atgcctcgaa tccggtcaaa cggttcaaca
    10261 cggccttcca gtttgcgatc gcggtatagg ccgtgacgaa gaaggaaagc gcgccctgca
    10321 cctgaccgaa ggccgaggcg atctgcatca gccccccaag cgtcatgctc ccaaagaaat
    10381 agaccgggct caccacgatg aaaggaaaga tgatggcgac ctgcgagtag ccggcagtga
    10441 ggaacgtgag ccgcttccga cgccgcatga tctcgtacca gttgcggatc accgatccga
    10501 agcggtcgcg gagccgcgcc tcttcggcct cttcgcccgc gagcagcgtg acttcctccg
    10561 cattctcgcg caagcgcacc agcgagaagc ggaaatcggc ctcatagcgc tgctgatcga
    10621 aatcgagctt gatcaggggg cgcccgacga gatgggtcac ccaggtgccg aggatggcgt
    10681 agatcagcgc cgcccagacc aaatagcccg ggatctcgaa cgccttcgac ccgatcatca
    10741 gcggcgtcgt ggctgacagg ttccacaaga tcacgatgaa ggacacgagc gtgacgatcg
    10801 agccgagcag agcgataccg atagtgagcg tgccgtcgac aaactcgcgg atgtcgtcgg
    10861 cgatgcgctg gtcggggtta tcagcctgat cccctttgag ccgcatgcgg taatgcgtgc
    10921 cttggccgag ccagcgcccg agatagcgct gcgtaagcca gttgcgccac cggatctgga
    10981 gccattgctg caggtagagc tgatagacgg cgcttacgat gaagaaagcg gcaagcaggc
    11041 tgaacacgcc gatctgccag atgaaggtgt cccagtcctt gttctgcagc gaggtgtaga
    11101 aggtgttgta ccaggcgttg aattggacgt tcagccagac ctggaacagc tggagcccga
    11161 tgacggcgga cagcagcacg cgtcccgccc agcgatcctc ggattggtaa taagggatgg
    11221 cgatgccggc gaagtctttg aacgtttttt tgagattggc cattcgctaa gggttccacg
    11281 agggccgggc cggcgacaat ctgccgacga tacggccaaa accgggcgct gaggaaatta
    11341 cgtttatgta aggctcgggt gattggaagg cctcatctga cccggtcaag gcaagggccg
    11401 agtcgaaaaa agaccccctc accgcgcgca agggcgcgcg tggtctcccc tagggaggag
    11461 cgggaagacg aggtaagcgc cgcacgacat ttccctctcc ctttttcagg gagagggctt
    11521 cccgtccggc gcgcgaagcg cggcgaatgg cgggaagggt gagggtgaac cttttgacgt
    11581 cgagaggggc cgagcccgcc gtagctccga aggagcgaaa gcgcggcgag gcgggggtga
    11641 gggtggtttg ttgaaccccg aactgtcttt ggtacctgtc agcggcggct gcgcaaaagg
    11701 ggcggcccga aggccgcccc ttgcgaatgt taaccgggtt cgcgatcgaa cggactacat
    11761 catgtccatg ccgcccatgc cgccacccgg catatgcgaa tgctcgtcct tcttcttcgg
    11821 cgactcggcc acgcccgcct cggtggtcac gagcagcccc gccacggagg ctgcgtcctg
    11881 caacgccgtg cgcacgacct tggccgggtc gatgatgccc ttggagatca aatcgccgta
    11941 ctcgttgttc tgagcgtcgt agccgaagga ggccgacttc tggtcggtca ccttgccgac
    12001 cacgatcgag ccctccacgc cggcattctc ggcgatctgg cggatcggag cttgcagcgc
    12061 gcgcctgacg atattgatgc cggcgtcctg gtcctggttg tcgcccttgg tcttgagggc
    12121 cttcgcggcc cagagcagcg aaacgccgcc gcccggcacg atgccttcct cgaccgccgc
    12181 gcgggtcgcg ttcaaggcat cgtcgacgcg atccttcttc tccttcacct cgacttcggt
    12241 cgcgccgccg accttgatca cggcaacgcc gccggcgagc ttggccagcc gctcctgcag
    12301 cttctccttg tcgtaatcgg aagtggtgtc ctcgatctgc tgcttgatct gggcgatgcg
    12361 ggcttggatg tcgggcttct tgccggcacc gtcgacgatg gtggtgtcgt ccttcgtgat
    12421 cacaactttc ttcgccttgc ccagcatgtt gacggtcacg ttctcgagct tgatgccgag
    12481 atcctcggag atcagctcgc cgccggtgag cacggcgatg tcctggagca tggccttgcg
    12541 gcggtcgccg aagcccggag ccttcaccgc cgcaaccttg agcccgccgc gcagcttgtt
    12601 gaccacgagc gtggcgagcg cctcgccctc gacgtcctcg gcaatcacca gcaacggatg
    12661 tccggactgc actaccgcct cgagcagtgg gagaagcgcc tgtaggcccg agagcttctt
    12721 ctcgtagatc agaatgtagg ggctctcgag ctccgccatc atcttctcgg cattggtgat
    12781 gaagtagggg gaaatgtagc cgcggtcgaa ctgcatgccc tcgaccactt cgagctcgct
    12841 atcgaggctc ttcgcttcct cgaccgttat cacgccctca ttgccgactt tctgcatggc
    12901 ctcggcgatc atgtcgccga tcgccttttc accattcgcg gcgacggtgc cgacctgtgc
    12961 gatctcgtcc gacgacttga ccttcttggc ctgccgcgca atgtccttga cgacctcggc
    13021 taccgccaaa tcgataccgc gcttcagatc catcgggttc atgcccgcgg cgaccgactt
    13081 caggccctcg cggacgatcg cctgcgccag cacggttgcc gtcgtggtgc cgtcgccggc
    13141 ggtgtcgttg gtcttcgagg cgacctcgcg cagcatctgc gcgcccatgt tctcgaactt
    13201 gtcctcgagc tcgatctcct tggctacggt cacgccgtcc ttggtcgagc gcggcgcgcc
    13261 gaacgacttt tccagaatca cgttgcggcc cttgggcccc aacgtgatct tcaccgcatt
    13321 cgagaggata tcgacgccgc gcatcatccg ctcgcgcgct tcggccgaaa acttcacatc
    13381 tttggctgcc atttcacttc gctccgatct tgtctcttga gttgctgttg agggtggtgc
    13441 ggcgctagcc gagaattccc atgatgtcgg actccttcat gatgagaagt tcctcgccgt
    13501 cgatcttgac ctcgctgccg gaccacttgc cgaacagcac cttgtcgccg gccttgaccc
    13561 ccatgggctg aagcttgccg tcttcgccgc gggcgccggg gccggccgcg atcacctcgc
    13621 cggtctgcgg cttctccgcc gcggtgtcgg gaatgatgat cccgcctttg gtcttctgct
    13681 cctcctcgag ccggcgcacg accacgcgat cgtgcaaggg tttgaatttc atggattttg
    13741 ctccgcgaac ttgcctggga aaattaagaa atgtgccgga ttggcactcg ccaacaacga
    13801 gtgccaacag atggcgagga gatactgtcg cgcccgcact ctgtcaagcc gggcaacctc
    13861 agctttgccg gctttgtctc actgcgcccg gcggcgcccg aaggagacga tgtagtccgc
    13921 cgaacggatc tggaagatcg ggtcgtccgc aggtcccgca atgccatcgg tcacattccc
    13981 gggaaggaag gaaaaggcat cgcacgccga ggccggcgcc agcgcctcga tgctgatctt
    14041 accgagcggc gtcgtttcgc gcccgtcctc gtcctcccaa cgttgcgtgg gatcgctggt
    14101 ctgatcgccg tcacgcccgc gaacggcctg aagctcgaag gagacagggc ctttgccgag
    14161 ccgttccttg atctcctctt cgtagaaatt gtcgcctttg ctcttggcct cttcgtccga
    14221 aagtccaagt tcgcccgcgt ccggtacgac cttgtatttg acgagggcct tctcgccatc
    14281 tgcattggtg aacgtgaagg catgcacgcc gaaatagccg acgccggcat agcttgcggg
    14341 aacgggatga ctgttgagcc atgccgcttg tcgcgtcgat tccgggtgcg cagcgaagaa
    14401 ggcatcgagc ttcgccttgt cgcccgaacc gaccgtctgc aggaactcga cgaacaaggc
    14461 gggagtcgtg acgaaaaaga caggcgccga gatcatcacg agatcggtga aggcgccgcc
    14521 gccgaggtcg aagcggatcg ccaacccgcg aacattgccc ttgacgttgt ccggcgcatt
    14581 cgcatttccg ccgccgagcg aaaagcgtgc cgtgatcggg acgggcttgg cgaaatgcgc
    14641 tgctttggag agcttggcgg catcgggcgc gggcgtgaac tggcccgtca ggcaaaatcc
    14701 tttggcgtga gcgctgcgcg ttccttgggg cttcccgaac acgccgttga gcgcgtcgac
    14761 cagatcggca ggggttggat ccgccgcgcg gacagtctcc gcggcggcga acaggtgcag
    14821 gccgagcagg gcggctgctc cgccaagagc aacgaaactg gctttcatgg tacgcgacct
    14881 catcgaatat atgtccggat gcgagagctt agcccaaaag tatgacgagg gggcatacgc
    14941 gttgggtcgc cacgtgatcg gcggcgcgta aaaccttcaa aaagccgcgg cttggtttcg
    15001 ccgcaccttg ctgctatcaa cattcaaagc ggcggcgaaa ttgctgttgc cttgaagggg
    15061 gctgccgccg catgattcta acgcgagggt gacaatttgg ctcgcacgac agccatggcc
    15121 acggggcgga gcgaaatctc gggaggcctt gccatgctcg acgtgatcgt gctcatcgcc
    15181 atggccgtca cggccgctgc gttcgccgtg ggcgtcattc tccattccgg aatcgcccag
    15241 atcccggccc tcatcgccgc cgcggcgctc tacatggtga tggcggcctc ctatctcatg
    15301 gtcgcgcgtt cgcgcccgag cggcgggagc ggcgcgcgcc ttggcgattt cgaggaggcg
    15361 ctggaaatca tcgacagaga tctgcagcgc atcgaccgcc tggaggagga gatctcccgt
    15421 ctcg
//
LOCUS       DASUQP010000130         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_880208,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000130 DASUQP010000000
VERSION     DASUQP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880208"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFY74_05885"
     CDS             <1..298
                     /locus_tag="VFY74_05885"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX5931265.1"
                     /translation="ADGAPRLLLKGTNFQIKVWSALLRLQPGQLISYGELARKIGQPK
                     AAQAVGRALSANPLGFIVPCHRVIRATGALGDYRWGKARRRAILAWETADQDAA"
     gene            730..1131
                     /locus_tag="VFY74_05890"
     CDS             730..1131
                     /locus_tag="VFY74_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5931266.1"
                     /translation="MSKMNPVVHFEMPYDDRERMAKFYQNAFGWQTQMLGEEMGNYVL
                     ATTTETDGSRPKEPGAINGGFFPKRPDLPAQHPSVVIAVDDIQAAMTKVAEAGGKVLG
                     EPMQIPGVGQYVSFFDTEGNRVSMLQPNPRD"
     gene            1187..2212
                     /locus_tag="VFY74_05895"
     CDS             1187..2212
                     /locus_tag="VFY74_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5931267.1"
                     /translation="MPVQELPPPFVLFRMVTGYYVSRAIHVAAKLGIADLLAEGARGH
                     DELARATSTHPDSLRRVLRLLAGAGVFTEEEDGKFALTPTGACLRSGVPGSMRAATLL
                     FGGITQEAWGDLLHSVETGEPAFRRVLGMDSFAYIAEHPDEAANFDAAMAGFTKRIAT
                     AVAEAYDFSPFHHIVDIGGGNGLLLAGILTAYPALAGIIFDLPHVAARAEEQIRELDL
                     ADRCEIVGGDFFKEVPSGGDAYLLKHVIHDWNDDRATEILRNCRRAMGAEATLLIVEG
                     VYPSRIDQSEESRGAAANDVNMLVCTGGRQRSEAEFRGLFDAAGFRLMRIVPDQAPVK
                     VIEGVCA"
BASE COUNT          399 a          763 c          753 g          387 t
ORIGIN      
        1 cgccgacggt gcgccgcggc tcctgctcaa gggcaccaac ttccagatca aggtctggtc
       61 cgcgctgctg cgcctgcagc caggtcaact tatctcctat ggcgagctcg cccggaagat
      121 cggtcagccc aaggcggcgc aggcggtcgg ccgggcgctc tcggccaacc ccttaggttt
      181 catcgtcccc tgccaccggg tgatccgggc aaccggggcg ctcggcgact accgctgggg
      241 caaagcgcgc cggcgcgcga tcctggcctg ggagacagcc gaccaggatg ccgcctgagc
      301 ggcgaagctt ccgatcgccc gccaaccgcc cgccgccgac ttccccggcc gggcggaagg
      361 cagcgcggag cgcgcgagtg gctccgccgt ggccgtcatg ccaaggcgcc gacccgcctg
      421 cacttcgcgc gatgtcagcc gttccagctg aacatcgcag ccataaacgc tgggaccatg
      481 aatctttgcg ctcaccgggg cccgctcagg cgatgtccga agatcagccg gtgtcggtcg
      541 acgccgtctc gtgcgtcctc tgactgagcg gaagctggga tcaggtgccg agcgggaagg
      601 ggtcgtcgac gcgctgccgc ttttgttcaa ggtgtggtcc ggcccgaaag acatggcgca
      661 ttggtggggc caaacggcgg cgcgccatcg ttcggcaaac gtctcgagcc atcggtaagg
      721 aggaccgtca tgagcaaaat gaaccccgtc gttcactttg aaatgccata cgacgaccgc
      781 gagcgcatgg cgaagttcta ccaaaatgcg ttcggctggc agacgcagat gcttggcgaa
      841 gagatgggca actatgtcct cgccacgacg accgagaccg acgggagccg ccccaaggag
      901 ccaggcgcga tcaacggcgg cttcttcccg aaaaggcccg acttgccggc gcaacatccc
      961 tccgtggtca tcgcggtcga tgacattcag gctgcgatga cgaaagtggc cgaagccggc
     1021 ggcaaggtcc tgggcgagcc gatgcagata ccaggcgtcg gtcagtacgt ctcattcttc
     1081 gacaccgagg gcaaccgcgt gagcatgctt cagcccaatc cgcgcgatta gttgggccga
     1141 agccgaaatg ggaaggagcg catagcccga acgggaggat ccttcgatgc ccgtgcagga
     1201 actgccgccg cccttcgtgc tctttcggat ggtcaccggc tattacgtct cgcgggcgat
     1261 ccacgttgcc gctaagctcg ggatcgctga tctgctcgcc gagggcgcgc gcggccatga
     1321 cgagctggcc agggcgacgt caacgcaccc ggactcgctt cgtcgggtcc ttcgactcct
     1381 cgccggcgcg ggcgtgttca ccgaggagga ggacgggaag ttcgcgctga cgccgaccgg
     1441 cgcctgcctg cgctcgggcg tgcccggttc gatgcgtgct gccacgctcc tgttcggcgg
     1501 catcacgcag gaggcctggg gcgatctgct ccacagcgtc gagaccggcg aacccgcctt
     1561 tcgccgtgtc ttgggcatgg attccttcgc ctacatcgcg gagcacccgg acgaagccgc
     1621 caacttcgat gccgcgatgg ccggctttac caaacgcatc gccaccgccg tagccgaggc
     1681 gtatgacttt tcgcccttcc accacatcgt cgatatcggc ggcggcaacg gcttgctcct
     1741 cgccggcatc ctcaccgctt acccggctct cgccggcatc atcttcgatc ttccccatgt
     1801 cgccgctcgg gctgaggagc agatccgcga gctcgatttg gccgatcgct gcgagatcgt
     1861 gggcggtgat ttcttcaagg aagtgcccag cggcggcgat gcctacctcc taaaacatgt
     1921 catccacgac tggaacgacg atcgcgccac cgaaattctg aggaactgcc ggcgtgcgat
     1981 gggcgccgag gccacgttgc tgatcgtcga aggcgtctac ccgtcgcgta tcgaccagtc
     2041 ggaggagagc cgcggcgccg ccgctaacga tgtcaacatg ctggtctgca cgggcgggcg
     2101 ccaaagatcg gaagcggagt tccgcggtct cttcgacgca gcaggcttcc gtctgatgcg
     2161 gatcgttccg gaccaagcgc cggtcaaggt gatcgaagga gtatgcgcct agcccgacgt
     2221 cggggagtct cgaaagccgc gcgggctgca agtgcctgtt ccgcaaaggt tgggatcggg
     2281 cgccgatgcg cgatgccgga cc
//
LOCUS       DASUQP010000131        10626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1833539, whole genome shotgun sequence.
ACCESSION   DASUQP010000131 DASUQP010000000
VERSION     DASUQP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833539"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(700..1857)
                     /locus_tag="VFY74_05900"
     CDS             complement(700..1857)
                     /locus_tag="VFY74_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX5931268.1"
                     /translation="MQRSYINPFPNGDRYRVIVIGDSLGEGLWSGLYRAFEEDKNLEF
                     IQRSKSNSGFARTDYYDWNEELAKLLKADTYQIAIVMFGGNDAQAVRSGKEWLKVGSE
                     GWREIYGKRVEAFIKQLRAAHIAVYWVGLPIMRSATQNADAEALNDVFREKAFINGAK
                     FVETWNGFTDEAGRYSPYGPDMTGQVKRLRADDGVHFTMRGYLKLAHFVEKDLRSDLN
                     LAKIERNIPLAGNEEEQAKVMGRDIVPSPSDPTQAQAQAPPPSLDPSAAEAGAATEQA
                     AGGETPQLQQSNVGEVSVVRPAIDQTLQAAQSMNPQGAPAAGPDTEMITSELGNGLTA
                     VATLSSVSDPSLASSRPRLPLSQRPYYKVLIKGEQLKPKAGRADDFAWPPS"
     gene            complement(2067..3413)
                     /locus_tag="VFY74_05905"
     CDS             complement(2067..3413)
                     /locus_tag="VFY74_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5931269.1"
                     /translation="MRIDQHAKSLILRAGFAALILIAVPGAGLADSKCVESLWPAAKG
                     AGVTRATFDRAFQGYAPDPEVIEAANYQPEYVKPIGEYIDRAVSDKRVATGTEKLAEY
                     QDLLAALEKRYGVDRHIIVAIWGVESNYGVQPGDKNVIRSLGTLICSGTKAKFARPQL
                     VSALKILQRGDITFEAMNGSWAGAMGHTQFIPTTYTAYAVDHDGDGKRDIWGNIPDAL
                     ASTASYLRVSKWRPGETWGYEVKLPNGFNPKRVNENTLKPLGEWQQMGVVRVNGEPFP
                     RASDKASLFAPEGTRGPSFLVLNNFRSILRYNVAKSYALAVGHLGDRLKGYGRFVQQW
                     PTDETHLSLAQRTELQQLLVARGIMTGEPDGVIGPATLEAVRTYQRAKSLPVDGFPSL
                     TILKMLRAEAPPPAPAGEGQTGSIGEAQAPAGGEAPPASAGEGLPAGGQPADVGQN"
     gene            complement(3461..4819)
                     /locus_tag="VFY74_05910"
     CDS             complement(3461..4819)
                     /locus_tag="VFY74_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5931270.1"
                     /translation="MATSALKGARLGLVLVAALLLPLGVEAAHDDVAAYKRAVEKSFG
                     QWLEALRPDAEAAGVSGETFDANVKGVKLDWSLPHLVLPNPAGPDGPPLPQALAATVK
                     PRHQPEFDIPANYFNKNTLNVLAGTGRGKMQQWAETLGAMQKQYGVPASIVVAIWGRE
                     TGFGKVDMPFDALSAIATQGFMGRRPEVFRKEVIAALKILQEGHASRAQMRSSWAGAM
                     GYTQFLPSDFEKYAVDFDGDGRRDIWNSVPDALGSTANSLHSQGWDGKQSWGYEVTLP
                     DGFDCTAQGPDKARPVAEWVKLGLARVKGRAFPEEKLQDPAFLVLPAGLKGPAFLATN
                     NFSVLKLYNNSDVYAIFVGHLADMIASGAPVAFAGEWKAVERLPRDRVQQFQEALVAK
                     GHDVGKVDGLAGFKTRRTIGLEEQRLGMKLTCYPSAALVDAVLKDARAEGAAAKAERP
                     AE"
     gene            complement(4910..5902)
                     /gene="galE"
                     /locus_tag="VFY74_05915"
     CDS             complement(4910..5902)
                     /gene="galE"
                     /locus_tag="VFY74_05915"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX5931271.1"
                     /translation="MSVLVTGGAGYIGSHMVLALLDAGEDVVVLDNLSTGFRWAVPSQ
                     TTFVAGDAGNRDLLLRLFLIHRIDAIIHFAGSIVVPDSIADPLGYYGNNTCKSRTLIA
                     AAVEAKIPHFIFSSSAAVYGMPQNNPVAEDARLEPISPYGTSKLMTELMLRDTAAAHD
                     LRYVALRYFNVAGADPEGRSGQSTARATHLVKVGCETALGERPYLEVFGTDYPTPDGT
                     CIRDYIHVADLARAHLDALRYLRAGGSSAVLNCGYGSGYSVLDVIASVKRVARSDFPV
                     HMGPRRAGDPAALVAKAERIGEVLGWEPRLNRLDTIVGHALAWEKRLIEYRAAS"
     gene            6018..6893
                     /gene="galU"
                     /locus_tag="VFY74_05920"
     CDS             6018..6893
                     /gene="galU"
                     /locus_tag="VFY74_05920"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX5931272.1"
                     /translation="MAARVRKAVFPVAGLGTRFLPATKAMPKEMLTVVDRPLIQHVVD
                     EARDAGIEHFIFVTGRNKSVIEDHFDRQFELEQVLAARGKTAELQAVTTDLPDAGNTS
                     FTRQQEPLGLGHAVWCARELVGNEPFALLLPDVLVQTPGKGCLAQMLDAYAEHGGNIL
                     AVEPVPDDQVKNYGVVSLGGKGDGRLLPIDGMVEKPKPGTEPSNLTILGRYILQPEIF
                     AILGAQEKGSGGEIQLTDAMLALLERQPFYALRYDGRAFDCGSRLGFLSANIAYALAR
                     PDLAKSVLAEIKKLL"
     gene            7055..8062
                     /locus_tag="VFY74_05925"
     CDS             7055..8062
                     /locus_tag="VFY74_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX5931273.1"
                     /translation="MTAGLRRDRLFRDDRGEAVASATRTLQLETEGLSALKAALAGEL
                     VDNFAEAVERLRNCRGRIIVTGMGKSGHVAQKTAATLSSTGTPSYFVHPSEASHGDLG
                     MITRDDVILAFSWSGETVELGNIVSYSRRFAVPLIAVTSNPGSTLAEAAELVLAMPQA
                     KEACPHGLAPTTSTVMQLALGDCLAIALLESKGFTARDFKTLHPGGQLGARLKFVGDL
                     MHKGDRLPLAQADAVMAEAIVVMTEKALGCLGVVDSDGLLRGIITDGDLRRHMGDGLL
                     ARHAHEIMTPAPKTVSPDLLASAALELINASKITTLFVVEDGRPVGIIHVHDLLRAGV
                     A"
     gene            8065..9567
                     /gene="glpD"
                     /locus_tag="VFY74_05930"
     CDS             8065..9567
                     /gene="glpD"
                     /locus_tag="VFY74_05930"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX5931274.1"
                     /translation="MADQSFDLLVIGGGIHGAAVARDAAGRGLRVMLAEKGDYACATS
                     SSSSKLIHGGLRYLERLEFGLVRESLVERSHLLNAAPHLVTPLRFLLPIYRWQRRQAL
                     TLRAGLALYDALSFGDGLPASGRLDDDDITQLPRLRQEELVSVLHYCDCMADDARLTL
                     AELLDARARGADIRNRRAVTAIAALDNGYRVELDERGHKRHVEARFIVNATGPFVASI
                     DAMTGVSPPARALTLVRGSHIVLPMPAPSHADAYTLQDEEERVVFVLPWLDQRFLIVG
                     TTDIPHQAEPGEPRCSAEEMAYLLAAYNRYFASPAGPVTHADIVHSWSGVRALHDEEG
                     VAPSRISRSPALSSAANGTGGFVTLYGGKLTTHRALAEDVLEALLRLGASAGGAWTKD
                     VPLHGGRLSLPELLDLAALGPNEISEATRRRWTLTYGDYLQALFGRIAAEPASAQEIA
                     PGVPRAELEYAVEVEDAMTAEDFLLRRTKLHLTLDQRERNAVARWFQSGA"
     gene            complement(9564..10016)
                     /locus_tag="VFY74_05935"
     CDS             complement(9564..10016)
                     /locus_tag="VFY74_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5931275.1"
                     /translation="MQTILALGAWNWLILAAILFALELLSPGIFLIWFGLAAAVTGLI
                     AFSSDISWQWQLLWFCALSLISVLLAAKYLRQHPLWSERPLLNQRALQLIGQSFDLVD
                     PIVNGRGSIKAGDTIWRVEGPELPKGARITVIGADGTLLKVTPATAGT"
     gene            complement(10037..10626)
                     /locus_tag="VFY74_05940"
                     /pseudo
     CDS             complement(10037..10626)
                     /locus_tag="VFY74_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341081.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
BASE COUNT         1873 a         3488 c         3314 g         1951 t
ORIGIN      
        1 ggttggtcgg attgtcgtct gcagcgccgt gcaccggatt gtcgtctgcg gcgccatcca
       61 ccatcggctt atgttcacgc ggtggcacat gttcacgtgg tgcaagccag ttcaccagac
      121 gcgcggcgac ggtatcgccc ggcagcgcct ctacaacagg agtcaaataa gagttgttgt
      181 aatgctcggt cgtgattgta tagccgccga cctgaacggg tcccgtattt gcggtcaggt
      241 tgatcttgac cttctggccg ccaccttaaa gcggcgtgac ttcgcaaatc ccctccagcg
      301 ccggaatctt gcctacagtc gcagcagagc aaatcggcgg ctgaatgagt tcgggcgaat
      361 cttcagcgcg caacgatccc ggctgtgcaa ggaatgcggc ggcgaacgcg agaatggcgg
      421 tccaatggac cggggcactc gcacaaccca actgcgatcc ggaagcagcg ctccgccgca
      481 cggaacgtct ccacaggagg tggacgttac cgcccatcaa acctcgacaa ccatcggttg
      541 tcaatacggc accctgacct tcagcacacg tcactggtaa tcggcaggcg tcgctggcgc
      601 actatcgacg tatcggcatt ggaaattccg aggttctaga agtgtgcagc tgcgggtggc
      661 cccaacaacc ggtaggcccg attgggcgcg ctttagcgct cagcttggcg gccaggcgaa
      721 atcgtcggcg cggccggcct tgggcttgag ttgctcgccc ttgatgagga ccttgtaata
      781 gggccgctgc gacagcggca gccgcggccg cgacgaggca agcgaagggt cggacacgga
      841 ggagagcgtg gcgacggcgg tcaacccgtt gccgagctcg ctcgtgatca tctcggtgtc
      901 gggtccggcc gcaggcgcgc cttgcgggtt catgctttgc gcggcctgga gcgtctgatc
      961 gatcgcaggc cttaccacgc tcacctcgcc gacattgctc tgctgcagct gaggcgtctc
     1021 tccgcccgcc gcctgttccg ttgcggcccc cgcttcggcg gccgaaggat cgagcgaagg
     1081 cggcggcgct tgcgcttgcg cttgcgtggg atcggacgga cttgggacga tatcgcgccc
     1141 catgaccttg gcctgctcct cttcattgcc ggctagcggg atattgcgct cgatcttggc
     1201 gaggttcaga tcgctgcgca ggtccttctc cacgaaatgg gcgagcttga ggtagccgcg
     1261 catggtgaag tggacgccgt catcggctct gagccgcttg acctgcccgg tcatatcggg
     1321 cccgtagggg ctatagcgtc cggcctcgtc ggtgaagccg ttccacgtct cgacgaactt
     1381 ggcgccgttg atgaacgcct tctcgcggaa cacgtcgttc agcgcctcgg catcggcgtt
     1441 ctgggtggca ctgcgcatga tgggaagacc cacccaatag acggcgatat gggcggcgcg
     1501 gagctgcttg atgaaggctt cgaccctctt gccgtagatc tcgcgccagc cttcgctccc
     1561 gaccttcaac cattccttgc cgctgcgaac ggcctgcgcg tcgttgccgc cgaacatgac
     1621 gattgcgatc tggtaggtgt cggccttgag cagcttcgcg agctcctcgt tccagtcgta
     1681 atagtcagtg cgcgcaaatc cgctattgga cttcgagcgc tggatgaatt cgaggttttt
     1741 gtcctcctcg aaggcgcgat agaggcccga ccacagaccc tcgccgagcg agtcgccgat
     1801 cacgatgacg cgatagcggt cgccattggg gaaagggttg atatagctgc gctgcacggg
     1861 gggcgcttgg gcgaggacag ggacaacccc aaagatcagg ccagccaaaa cggcggccac
     1921 aaggctccgg ccgcccgcgc ggcttgagcc gaaccgagaa accttaccaa gcattgtcgc
     1981 cagggtccgt caccggaccc accctcaacc cgcccccagc ccccacactg gtagtccctt
     2041 ccgggttaaa cgccaagccc ctcgcctcag ttctggccga catcggccgg ctgcccgccg
     2101 gccggcagcc cctctcccgc ggaggccggc ggcgcttcgc cgccagcggg tgcttgcgcc
     2161 tcgcctatgc tcccagtctg gccctcgccg gcgggcgcgg ggggaggcgc ttcggcccgg
     2221 agcattttca gaatggtcag gctcgggaag ccgtccacag ggagcgactt cgcgcgctga
     2281 taggtcctga ccgcctcgag cgtcgcgggg ccgatgaccc cgtcgggctc tccggtcatg
     2341 atcccgcgcg cgaccaggag ctgctgtagc tcggtcctct gtgccagcga aagatgagtc
     2401 tcgtcggtcg gccattgctg cacgaaccgc ccgtatccct tgagccgatc accgagatgg
     2461 cccacggcaa gcgcatagga cttggccacg ttgtagcgga ggatcgaacg aaaattattg
     2521 agcacgagga acgaaggccc tcgcgttccc tccggcgcga acagcgaagc cttgtcggac
     2581 gcccgcggga acggctcgcc gttcactctg acgacgccca tctgctgcca ttcgccgagc
     2641 ggcttcagcg tgttctcgtt gacgcgcttg ggattgaagc cgttcggcag cttgacctca
     2701 tacccccagg tttcgcccgg ccgccacttc gacaccctga gatagctcgc cgtcgaagcg
     2761 agcgcatcgg ggatgtttcc ccagatgtcg cgcttgccgt cgccgtcatg atccacggcg
     2821 taagcggtat acgtggtggg gatgaattgg gtatggccca tggcgcccgc ccacgagccg
     2881 ttcatggctt cgaaggtgat gtcgccccgt tgcagaatct tcagagccga tacgagctgg
     2941 ggtctggcga atttggcctt ggtgcccgaa cagatcagcg tgccgagcga acggatcacg
     3001 ttcttgtccc cgggctgcac gccgtagttc gattcgaccc cccaaatcgc gacgatgatg
     3061 tggcggtcca cgccgtagcg cttctcgagc gcggccagca gatcctgata ctcggcgagc
     3121 ttttccgtgc cggtcgcgac ccgcttatcc gagacggccc gatcgatata ttcgccgatc
     3181 ggcttcacat attcgggctg gtagttggcg gcctcgatca cttccgggtc gggcgcgtat
     3241 ccctggaagg cgcgatcgaa tgtggcgcgg gtgacgccgg cgcctttggc cgcgggccac
     3301 aagctctcca cgcatttgct gtcggcgagg cccgcgccgg gcacggcgat gaggatcagg
     3361 gcggcaaatc ccgcccgcag gatcaatgac ttagcgtgtt gatcgattcg catgcagcga
     3421 cactaacaag aggggcgggc aaaacctagg caattgggct ttattcagct gggcgctcgg
     3481 ccttggcggc ggcgccctcg gcgcgagcgt ccttcagcac ggcatcgacg agggccgcgc
     3541 tcggatagca agtcagtttc atgccgagtc tctgttcctc gagcccgatc gtcctccgcg
     3601 tcttgaagcc ggcgaggcca tcgaccttgc ccacgtcatg gcccttcgcc accagcgcct
     3661 cctggaactg ctggacgcgg tcgcgcggca agcgctcgac cgccttccat tcgccggcga
     3721 acgcgaccgg cgctccggac gcgatcatgt cggcgagatg gccgacgaaa atcgcgtaga
     3781 cgtcggagtt gttgtagagc ttcaacacgg agaaattgtt ggtggccagg aacgctggcc
     3841 ccttgagccc cgccggcaga acgaggaatg ccgggtcttg aagcttctct tcaggaaagg
     3901 cgcggccctt cacccgcgcc aggccaagct tcacccattc ggcaaccggt cgcgctttgt
     3961 caggcccctg cgccgtgcaa tcgaaaccgt cgggcagcgt gacctcatag ccccaactct
     4021 gcttgccgtc ccatccctgg ctgtgcagcg aattcgcagt cgagccgagc gcatcgggca
     4081 ccgaattcca gatgtcgcgc cgaccgtcgc cgtcgaaatc gacggcatat ttttcgaaat
     4141 cggatggcag gaactgggtg tagcccattg cgcccgccca ggagctacgc atctgggcgc
     4201 ggctggcgtg tccctcttgg aggatcttga gcgcggcgat cacctctttc cggaacactt
     4261 cgggtcggcg acccatgaag ccctgcgtgg caatggccga aagcgcatcg aacggcatgt
     4321 ccactttgcc gaaacccgtc tcgcgccccc agatggcgac gacgatgctt gccggcacgc
     4381 cgtattgctt ctgcatggcg ccgagcgttt ccgcccattg ctgcatcttg ccgcgacccg
     4441 tgccggcgag aacgttgagc gtgttcttgt tgaaatagtt ggcggggata tcgaactcgg
     4501 gctgatgcct cggcttcacg gtcgcagcaa gggcttgcgg caaaggcgga ccgtcaggcc
     4561 ctgccgggtt gggcaggacg agatgcggca gcgaccagtc gagcttcact cccttgacgt
     4621 ttgcatcgaa ggtttcgccc gagacccccg cggcttcggc atcgggccgg agcgcttcga
     4681 gccactggcc aaagctcttc tcgaccgcgc gcttataggc ggcaacatcg tcgtgagccg
     4741 cctctactcc caagggcaac aacaaggcag ccacgaggac aaggccgagc ctcgcgccct
     4801 tcaatgccga tgtcgccatg ccactccccc gagttccccc aaaagattca ccgacctcac
     4861 cctttctctc tcgcgccttt gcggcgcggc aaaggcaaaa tgacaagggt caagaggcgg
     4921 cacgatattc gatcagccgc ttttcccagg cgagcgcatg gcccacgatc gtgtcgaggc
     4981 ggttgagcct gggctcccag ccgagcacct cgccgatgcg ctcggccttc gccacgagcg
     5041 ccgccggatc ccccgcgcgg cgcggcccca tgtgaacggg aaaatcgctt cgcgcgactc
     5101 gcttgaccga tgcgatcacg tcgagcaccg agtagccgct gccgtagccg cagttgagta
     5161 ccgcgctgct tccccccgcg cggagatagc gcagcgcatc gagatgggca cgggcgagat
     5221 ccgccacgtg aatataatcg cggatgcagg tgccgtccgg cgtcggataa tcggtgccga
     5281 acacttcgag atagggcctc tcgccgagag cggtttcgca gccgaccttc acgagatggg
     5341 tagcgcgcgc cgtcgattgt ccgctccgcc cctccggatc ggcgcccgcc acattgaaat
     5401 agcgcagtgc cacatagcgc aaatcatgcg cggccgccgt gtcacgcagc atgagctcgg
     5461 tcatgagctt ggacgtgccg tagggcgaga tcggctcaag tcgcgcatcc tcggcgacgg
     5521 gattgttctg cggcatgccg tagaccgccg cactcgagga gaagatgaaa tgcggaatct
     5581 tggcctcgac ggcggcagcg atgagcgtgc gcgatttgca cgtgttgttg ccgtaatagc
     5641 cgagcggatc ggcgatcgag tccggcacga cgatcgatcc ggcgaaatgg atgattgcgt
     5701 cgatgcgatg gatcaagaac aaccggagca gaagatcgcg gttgccggca tcgcccgcga
     5761 caaacgtcgt ctggcttggc accgcccaac ggaacccggt cgacagattg tcgagaacca
     5821 cgacgtcctc accggcgtcc agcagcgcga gcaccatatg gctcccgatg tagcctgctc
     5881 cgcccgtgac cagaacactc atggaacaac cttattacac ggcaattcgt ggcttgctat
     5941 cggatcgcgg ttgagagagt tctaactttc gccgtttaac cccgctccac cggcgagatc
     6001 gatttaacga ggaccggatg gctgctcgcg tccgcaaggc cgtgttcccc gtggcagggc
     6061 tcggcacccg cttcctgccc gcgaccaagg caatgccgaa ggagatgctg accgttgtcg
     6121 accggccgct catccagcat gtcgtcgacg aggcaaggga cgcaggcatc gagcatttta
     6181 ttttcgtcac gggccgcaac aagagcgtca tcgaggacca tttcgaccgg caattcgaac
     6241 ttgagcaggt gcttgccgcg cgcggcaaga ccgccgagct tcaagcggtg accaccgatc
     6301 ttccggacgc tgggaacacg agcttcaccc gccagcagga gccgctcgga ctgggccacg
     6361 cggtctggtg cgcgcgcgag ctggtcggca acgagccttt cgcgcttctc ctgcccgatg
     6421 tcctggtgca aacacccggc aagggctgcc tcgcccagat gctcgacgcc tatgccgaac
     6481 atggcggcaa tatccttgcg gtcgagccgg tacccgacga ccaggtgaag aattacggcg
     6541 tggtcagtct cggcggaaaa ggcgacggtc gcctgcttcc catcgacggc atggtcgaga
     6601 agcccaagcc cggaaccgag ccatccaacc tcaccattct cggccgctac atccttcagc
     6661 ccgagatctt cgcgattctc ggggcgcagg aaaaaggctc gggcggcgag atccagctta
     6721 ccgacgcgat gctggcgctg ctcgagcgtc aacctttcta cgcgctccgg tatgacggac
     6781 gcgccttcga ttgcggttcc aggcttgggt ttctcagtgc caatatcgcc tatgcccttg
     6841 ctcggcccga ccttgccaag tccgtcctgg ccgagatcaa gaagctgctg taaccgccca
     6901 aaacctccgc gcttcatccc gctcgtggcg cctctctctg accttattgg agagctagag
     6961 aacgggcggt aaagggccgt ttcgcccttt gtcgaaaggt cctcccaagg accggttccg
     7021 tgctaaagtc tggcgcggat gacgggggaa caatatgaca gccggtctta ggcgggatcg
     7081 cctgttccgc gacgatcgcg gcgaagcggt tgcctcggcg acgcgcacgc tccagctcga
     7141 gaccgaaggc ttgagtgcac tcaaggccgc gctcgccggc gagctcgtgg acaatttcgc
     7201 cgaggcggtc gaaagattgc gcaattgccg cggccggatc atcgtcaccg gcatgggcaa
     7261 gagcggccat gtggcgcaga agacggccgc cacgctctct tcgaccggca cgccctccta
     7321 tttcgtgcat ccaagcgaag caagccacgg cgatctcggc atgatcacgc gcgacgacgt
     7381 gatcttggcc ttctcctggt cgggcgagac ggtcgagctc ggcaatatcg tctcctattc
     7441 gcggcgcttc gccgttcctc tcatcgcggt cacgtcgaac cccggctcca cgctggcgga
     7501 ggccgccgag ctcgttctcg cgatgccgca ggccaaggaa gcctgtcccc acgggcttgc
     7561 gcccaccacc tcgacggtga tgcagcttgc gctcggcgac tgtctcgcca tcgctctcct
     7621 cgagagcaag gggttcaccg cgcgcgactt caagaccttg caccccggcg ggcagctcgg
     7681 ggcgaggctg aaattcgtcg gcgacctgat gcacaagggc gatcgcctgc cgctcgcgca
     7741 ggccgacgcg gtcatggccg aggccatcgt cgtcatgacc gaaaaggcct tgggctgcct
     7801 cggggtggtc gatagcgacg gcttgctcag gggcatcatt accgacggcg atctccgccg
     7861 ccatatgggc gacggtctgc tggcccgcca cgcccatgag atcatgacgc ccgcaccgaa
     7921 gacggtgtca cccgatcttc tggcaagcgc cgcgctcgaa ttgatcaacg cctcgaagat
     7981 caccacgttg ttcgtggtcg aggacggacg ccccgtcggc atcatccacg ttcacgatct
     8041 gcttcgcgcc ggcgtcgcct agcgttggca gatcaatcct tcgatcttct cgtcatcggc
     8101 ggcggaattc acggggcggc ggtcgcacgc gacgccgcgg gccgcgggct aagagtgatg
     8161 ctcgccgaga agggcgacta cgcctgcgcc acctcctcct catcgtccaa gctcattcac
     8221 ggcggcctgc gctatctcga gaggctggaa ttcggtcttg ttcgcgaatc tttggtcgag
     8281 cggtcgcact tgctcaacgc cgcgccccat ctcgtcacgc ctttgcggtt tctgctcccc
     8341 atctatcgct ggcagcggcg gcaggcgctc acgctgagag ccgggctcgc tctttacgat
     8401 gccctgtcct ttggcgacgg gttgccggca agcggcaggc tcgatgacga cgacatcacg
     8461 cagcttccga ggcttcgcca agaggagctc gtctcggtcc tgcactattg cgactgcatg
     8521 gccgacgacg cgagactgac gctcgccgag cttctcgatg cacgcgcacg cggcgccgac
     8581 atacggaacc gtcgcgccgt gaccgcgatc gcggccctcg acaatggtta tagggtggaa
     8641 ctcgatgagc gcggccacaa gcgccacgtc gaggcacgct tcatcgtcaa cgcgacgggc
     8701 ccgttcgttg caagcatcga tgccatgaca ggtgtaagcc cgcccgcccg cgctctgacg
     8761 ctcgtgcgcg gcagtcacat cgttctgccc atgcccgcac cttctcacgc cgacgcctat
     8821 acgctccagg acgaagagga acgcgtcgtg ttcgtcctgc cttggctcga ccagcggttc
     8881 ttgatcgtcg gcacgactga catcccccat caggccgagc cgggtgagcc gcgctgctct
     8941 gccgaggaga tggcctatct gctcgccgcc tataaccgct atttcgcgag ccccgccggg
     9001 ccggtcaccc acgccgatat cgtccatagc tggtctggcg tgcgcgccct ccatgacgag
     9061 gaaggcgtag cgccaagccg catcagccgg agccctgcgc tgtcttcggc cgccaatggc
     9121 acgggcgggt tcgtcacact ctatggcggc aagctgacca cgcaccgcgc tctggccgaa
     9181 gacgtgctcg aggccctgct tcgcctgggg gcaagcgccg gaggcgcctg gaccaaggac
     9241 gttcccctgc acggcgggag gctgtcgcta ccggagctgc tcgatcttgc cgcgttgggc
     9301 ccgaacgaga tttcagaggc gacgcggcgc cgctggacgc tcacctatgg cgattatctc
     9361 caggctctat tcggtcgcat tgccgcagag ccagcatcag cgcaggaaat cgcgcccggc
     9421 gttccgcgcg ccgagcttga atacgcggtt gaagtcgagg atgccatgac cgccgaagac
     9481 ttcctgcttc gccgcaccaa gctccacctg acgctcgacc agagggagcg caacgcggtg
     9541 gcacgctggt ttcaaagcgg cgcttaagtc ccggccgtgg ccggcgttac cttaagcagc
     9601 gtgccgtcgg cgccaatcac ggtgatgcgt gcgcccttgg gcagttctgg gccttccacc
     9661 cgccagatcg tatcgcccgc cttgatgctt cctctcccat tgacgatggg atcgacgaga
     9721 tcgaaagact ggccgatgag ctgcaatgca cgctgattga gcaacggccg ctcgctccac
     9781 agcggatgct gacgcagata cttcgccgcg agaagcaccg agatcagcga caatgcgcag
     9841 aaccagagga gctgccactg ccaggagata tcggaagaga aggcgatgag gccggtcacc
     9901 gccgcggcga ggccaaacca aatgaggaaa ataccgggcg acaacagctc gagtgcgaac
     9961 aagatcgcgg cgaggatcag ccaattccac gcaccgaggg cgaggatcgt ttgcatgggt
    10021 cgcgcctcgc ctttgcttag acggccggga cggagccgcc cgcgcgaggt cgcgatttgg
    10081 cctgacccgg ctgcggggct gagcccgaat tgccgccacc tgcaaaggct tcgcgggcga
    10141 tctcggagac gccggcgatg gcgccgatca ccgaggaagc ttcgagcggg agaatcagca
    10201 ctttctggtt cttggcctcg gcgagcgcct tcagggcacc aacataattg ttggccacga
    10261 agtagttgat cgcctgcgcc tgcttcgcgc cttccgcaat caggatcgcc gactgccgct
    10321 cgccttcggc cacgagaacc gccgccctct tgtcgcgctc ggccttcatt tgccgcgcca
    10381 tggcgtcgac cagatcgcgc ggcggctcga tatctttgat ccgatacggg tgaccttgac
    10441 gccccaatta tgggtggcgt cgtccaccac tttgagcagg cggtgattga tctcatcgcg
    10501 ctcggacaga agcttgtcga gatccatcga tcccatcacc gtgcggatat tggtcatggt
    10561 caagttgagg atggcgaggt tgagattggc gacttcatag cttgcctgcg ccgcgtcgag
    10621 cacttg
//
LOCUS       DASUQP010000132         8264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1833822, whole genome shotgun sequence.
ACCESSION   DASUQP010000132 DASUQP010000000
VERSION     DASUQP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833822"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFY74_05945"
     CDS             complement(<1..454)
                     /locus_tag="VFY74_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931276.1"
                     /translation="MSLISRAMWSPLFLPATANVRNQDSVVSTRHFFVIAPGDSENTE
                     YVKICNGYARSGATRRRIGARQNPPKLKRFLKFAFFPHSSRDDIVFDSMTQIRSTALQ
                     TAAEPPSDEGPPPSTSERAMRRRPGHYLDALNPEQREAVETLDGPLLVL"
     gene            471..836
                     /locus_tag="VFY74_05950"
     CDS             471..836
                     /locus_tag="VFY74_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931277.1"
                     /translation="MVLAALGFAVLAGGAIEPAQAACQLIRATHSAGSKADAARSSQA
                     LVLQSAAELKRARRWSYITLYAYRVKGDPFWKAVRPQGVPRQAQLKPDIVTARFYTTC
                     FTGVVVPFVCTTGSSVCGQ"
     gene            1015..1674
                     /locus_tag="VFY74_05955"
     CDS             1015..1674
                     /locus_tag="VFY74_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX5931278.1"
                     /translation="MLKSCVSFPKYMLSERVADGCLHAVGVAASLVAFTVLLVIGVKS
                     DTALWIVSLTIYGLALVATFSFSAGYHLVALHRPRLKEWLRRFDHAAIFLMIAASYTP
                     FVLIEMNTAFGHSLLAVVWAIAAVGIVLQLFAPRYLEGLSVALYLVQGWAVLAALEPL
                     MSALPGRVLTLLMAGGVLYTAGVVFHLWERLPYQNAIWHGFVLVAASCHFAAVIVVVA
                     L"
     gene            1730..3130
                     /locus_tag="VFY74_05960"
     CDS             1730..3130
                     /locus_tag="VFY74_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5931279.1"
                     /translation="MTAVMAEGQDAKRALFALLRAAFGARFSEGEALCEQHANTLTWL
                     AREPPDAVLFAESEAEVADCVKHCAEARVPVIPFGTGTSLEGHVNAPFGGVSLDLSRM
                     NRILAVNEADLDCTVEPGVTRKALNDHLRGCGLFFPVDPGADASLGGMAATRASGTNA
                     VRYGTMRDAVLGLNAVLADGSVIRTGGRARKSSAGYDLTRLIVGSEGTLGIITSLTLK
                     LYGIPETIVSAVCPFASIEGACNATIAALQIGLPLARIELLDEVQMRACNAYSRLTLR
                     ETPTLFLEFHGTPASAREQVEAFAAIARAEGGGEFAYAERQEERSKLWQARHDAYWAA
                     RGLRPGAEVLSTDVCVPISALANCVTQTRQDIDRFGLLAPIVGHVGDGNFHVLPLIDP
                     ADAEERRKVQALLDRLVERALAAGGTCSGEHGIGQGKIAYLAAEHGAGVKAMIAIKKA
                     LDPLNILNPGKIFALP"
     gene            3206..7312
                     /locus_tag="VFY74_05965"
     CDS             3206..7312
                     /locus_tag="VFY74_05965"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5931280.1"
                     /translation="MNSLLLTLAALLILVLSALFAAPLVIDWNNYRAVFETQAANLLG
                     RDVKVGGKVHLILLPAPELRFDDIKVADQDGRLDRPFLEAHSFEAWLNIGALLTGTIE
                     ARKITIVGPILRLDLKADGTGNWSDVGRRGVALPFAPKDVMLDEVSVSGGRIEITRQD
                     QPQFAVENINGQASAQSLSGPYKVSANYAFKGRPQELRFSTSAPDPAGLFRVKSALRD
                     LDRDTSYILDGGVTGLGAVPAFDGKIVVRTAKVLTDDEEGEAANSESVELSDKASREP
                     ASRFELKGPVKATPDRAELPGFDLTLHAKGHPQIFKGKLTLDFGERIEGAAELAAGFI
                     DLDALFGVPGAEERPSPATVLYEFADEVLAQAAALGDGTLALVIEQAGLGGDLVGAIN
                     MTLAAKDGVVTIGQLKATLPGENRIEASGSLKRGDFGPVFAGPVKVEGSGLRPLTRWA
                     AGDRDVSGQASAGDFSVMANAVVGDGALMLSDASGELSGTKFRGALRLKGGERRLIEI
                     DLDSDRLDLREVIDEGPLWRSWLTASQPAAPAAASNGATAAIDGGGATGTEDAAAMGQ
                     DLFKRLRGDDMRVTLRIGELLLPDIPAGKLDARFALQGGTLAVEQLDFAAADALTLNG
                     KGRIERLDEAPSGGVDFALRAADASSLKIAAGLFGLPEKVSGSEHLSVLAPLDLNVKL
                     KATREGDLTNAAVELGGKAGTSDIVLTARALGDPAKLGEAKIDVSAKVLGEKPQALLV
                     LLFPDLPLERIATPAGSKGRLIVKFAGVPNAKVTGKAALETEPIEVAFVGQGSLQPNG
                     LSLEGKAAAVSKDASAALTLIGLESPPSAAGVPLALRLDVAKSGATIDLNGITGSIAG
                     ENVTGSVQLDRSGAKTRFSLKGSAGSVSLPSLLGVLMAWHRTPSTEEMLGTISTNTSD
                     VWPSRGFSLSLLEEVEGAISLDAKTLALGSAVKVDEATLAASIGKDGLDVTALTGRLF
                     GGELAASGSLAPRGNGAELRARAEVKGGKLEALAKSLTGSALARGPFDLAFNVQGEGL
                     SPPGVVAGLSGEGTLTLGPGALQSFSAAPLRGVAATAAKKSIKADKEEIEAEAESVRE
                     RITKGTYKFAPVELAFDIKNGTLRLAPATLSSAGAETKINGFVELASLKLDSEWAMGL
                     TGDGVKDVPPVNLVFAGALNKAGAISPAIDTGAIEAYLTMRRMQEDVERLETLDVSGR
                     TAPAEAEPEGLAITLPDDSPAEASSPSTPELAEPSAWSAETETPEEPASQPSETSTTA
                     LPSALELLGTSEEAAVETETPATGEPAALSEPQAAPEAPASATLGPAAEPAPAPAEAA
                     TASPEAQPSPPAAATEKPPRKRSASKPRRQKQEAPDAWRKNVPIFGGG"
     gene            complement(7309..7545)
                     /locus_tag="VFY74_05970"
     CDS             complement(7309..7545)
                     /locus_tag="VFY74_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX5931281.1"
                     /translation="MPPSRKKRSLTIARHRTSVTVEEPFWDALGEIARAEGKSVAAIV
                     GEIDRRRSATDREAVSLSAAIRLYVLERTKRRGL"
     gene            complement(7563..>8264)
                     /locus_tag="VFY74_05975"
     CDS             complement(7563..>8264)
                     /locus_tag="VFY74_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5931282.1"
                     /translation="VGTGLNTHPGFAELFASKIAGMTKLPFVTARNKFEALASHDAYV
                     FAHGALTASAVGLFKIANDIRLLGSGPRSGFGELLLPENEPGSSIMPGKVNPTQCEAV
                     TMVAAQVIGNNATIAFAGSQGHLELNVFKPVLAYAMLQSIRLLADVADSFTENCVIGI
                     EANHPRVKEHMQRSLMLVTALAPHIGYDKASEIAKSAHKRGTTLREEAIRLGHVNAED
                     FDRLVRPEDMTGPKR"
BASE COUNT         1392 a         2707 c         2726 g         1439 t
ORIGIN      
        1 cgagaacgag aagcgggccg tcgagcgtct cgaccgcctc gcgctgctcg ggattgagcg
       61 cgtcgagata atggccgggg cgcctgcgca tggcgcgttc cgaggtggag ggcggcggcc
      121 cctcgtcgga aggcggctcg gcggcggttt ggagcgcggt ggagcgaatc tgtgtcatcg
      181 agtcgaaaac tatatcatcg cgtgagctgt gaggaaaaaa cgcgaacttc agaaagcgct
      241 tgagcttggg cgggttctgc cgggctccga tccttcggcg ggtcgcgccg gaacgcgcgt
      301 agccgttaca gattttgaca tattctgtat tctccgaatc accaggcgcg atgacgaaaa
      361 aatgccgcgt ggaaacgaca gaatcttggt tgcggacgtt cgccgtggct ggcaaaaaca
      421 agggactcca catagctcgg gagatgaggg acatgcttca aggcttgagg atggtattag
      481 ctgcgcttgg ctttgcggtc cttgcgggcg gcgcgatcga gccggcgcaa gccgcatgcc
      541 agttgatcag ggccacccat agcgccgggt cgaaggccga cgcggcgcgg agctcgcagg
      601 cgctcgtcct acaaagcgct gccgagctga agcgcgccag gaggtggtct tacattaccc
      661 tctatgcgta cagggtgaag ggcgacccgt tctggaaagc cgtccgtccc caaggcgtgc
      721 cgaggcaagc ccagctcaag cccgacattg tgacggcgcg cttctacacg acctgcttca
      781 ccggcgtcgt tgtcccgttc gtttgcacca cgggttcgag cgtctgcggc cagtagccgc
      841 attccttgat ggccgaagag gcgtcccggg cgcgcgcctc gcgcgcccga tacgctggcg
      901 cttttggcgc ttgaacattc ggcgcggtga gaaattgtcg ccttatcctt gccacagggc
      961 gtggtaagct cgcggtcgaa catgtgtgct gcgataactg gagccggtgc ggtgatgctc
     1021 aagagttgcg tttcgtttcc caaatatatg ctcagcgagc gcgtggctga cggctgcctt
     1081 catgccgtcg gcgtcgcggc cagtctcgtc gcgttcaccg tgctcctcgt catcggcgtg
     1141 aagtcggaca cggccttgtg gatcgtgagc ctgaccattt acgggctcgc attggtcgcg
     1201 accttcagct tttccgccgg ctaccatctc gtcgcgctcc accgcccaag gctcaaggag
     1261 tggttgcggc ggttcgacca cgccgccatc ttcctgatga tcgcggccag ttacacgccc
     1321 ttcgtcctca tcgaaatgaa cacggccttc gggcactcgc ttcttgccgt ggtgtgggcg
     1381 atcgccgccg tcggcatcgt gcttcagctt ttcgcgccgc gctatctcga gggactgtcc
     1441 gttgccctct atctggtgca gggctgggcc gtgcttgcgg ccttggagcc gctcatgtcg
     1501 gcgctgccgg ggcgcgtgct caccctgctc atggccggtg gcgtgctcta cactgccggc
     1561 gtggtgttcc atctctggga gcgcttgccc tatcagaacg ccatctggca cggtttcgtg
     1621 ctcgtcgcgg caagctgcca tttcgcggcc gtgatcgtcg tcgtcgcgct ctgacctttc
     1681 gccctgaaaa gcctaagcat ccaaggccga agcaccctag aatagcgcca tgaccgcagt
     1741 catggccgaa ggacaggacg ccaagcgcgc gctcttcgcc ctgcttcgcg cggccttcgg
     1801 cgcgcgcttc tcggaagggg aggcgctctg cgagcagcat gccaatacgc tcacctggct
     1861 cgcgcgcgag ccgccggacg ccgtcctgtt cgccgagagc gaggccgagg tggcggactg
     1921 cgtgaagcac tgcgccgaag cccgcgtgcc ggtcatcccg ttcggcaccg gcacctcgct
     1981 cgaaggccat gtcaacgccc cgttcggcgg cgtctcgctc gatctgtcgc gcatgaaccg
     2041 catcctcgcc gtgaacgagg ccgatctcga ctgcaccgtc gagcccggcg tcacccgcaa
     2101 agcgctcaac gatcatttgc gcggatgcgg tctgttcttc cccgtggatc ccggcgccga
     2161 tgcgagcctc ggcggcatgg cggcgactcg cgcctcgggc accaacgccg tgcgctacgg
     2221 caccatgcgc gacgccgttc tcggactgaa cgcggtgctc gccgacggga gcgtgatcag
     2281 gaccgggggc cgcgcgcgaa aatcttcggc cggctatgac ctcacccgac tcatcgtcgg
     2341 ctctgaaggc acgctcggca tcatcaccag cctcacgctc aagctctacg gcatccccga
     2401 gacgatcgtc tcggcggtct gtccgttcgc ctcgatcgaa ggtgcgtgca acgccacgat
     2461 cgccgcgctt cagatcggtc tcccgcttgc ccgcatcgag cttctcgacg aggtgcagat
     2521 gcgcgcctgc aacgcctatt cgcgcctcac cttgcgcgag acgccgacac tgttcctcga
     2581 gtttcacggc acccccgcct cggcgcgcga gcaggtcgag gccttcgcgg cgatcgcgcg
     2641 tgccgagggg ggcggggagt ttgcctatgc cgagcggcag gaggagcgga gcaaactctg
     2701 gcaggcgcgc cacgatgcct attgggcggc gcgagggttg aggcccggcg ccgaagtgct
     2761 atccaccgat gtctgcgtgc cgatttcggc gcttgcaaat tgcgtcacgc aaacgcgcca
     2821 ggacatcgac cgcttcggcc tgcttgcccc gatcgtcggc catgtcggcg acggcaattt
     2881 tcatgttctg ccgctgatcg atcccgccga cgcggaggag cggcggaagg tacaggcctt
     2941 gctcgaccgt ctggtcgagc gggcgctcgc cgcgggcggg acctgtagcg gcgagcacgg
     3001 gatcggccag ggcaagatcg cctacctcgc cgccgagcat ggcgcgggcg ttaaggcgat
     3061 gattgccata aaaaaggcgc tcgatccgtt gaatatcctc aatcctggca agatttttgc
     3121 gttaccatga cgcgcgtcgt gggtgaccga atcttcggcg cgaaattgcg cggttagcgc
     3181 gcccgagcct tgggtctaga cggcggtgaa ctcactcctg ctgactttgg cggcattgtt
     3241 gattctggtc ttgagcgcgc tctttgctgc gccgctcgtc atcgactgga acaactaccg
     3301 cgcggtgttc gagacccaag cggccaacct gctcggacgc gacgtgaaag tcggcggcaa
     3361 ggttcacctc attctcctgc cggcgccaga gctccgattc gacgacatca aggtggccga
     3421 tcaggatgga cgcctcgacc ggccgttcct cgaagcccat tcgtttgaag cctggctcaa
     3481 tatcggcgcg cttctcaccg gcacgatcga agcgcgcaag atcaccatcg tcggcccgat
     3541 cttgcgcctc gatctcaagg cagacggcac gggcaattgg agcgatgtgg gacgccgcgg
     3601 cgttgcgctt cccttcgccc caaaagacgt gatgctcgac gaggtcagcg tcagcggcgg
     3661 caggatcgag atcacaaggc aagatcagcc gcaattcgcc gtggagaaca tcaacggcca
     3721 agcgagcgcc caatcgttgt cggggcctta caaagtctcc gccaactatg cgttcaaggg
     3781 acgaccgcag gagcttcgct tctcgaccag cgcgcccgac ccggccggac tgttccgcgt
     3841 caagtcggcg ctgcgcgacc tcgatcgcga cacgagctac atccttgacg gcggtgtgac
     3901 ggggctcggc gccgttcctg ccttcgacgg taagatcgtc gtgcgtacgg cgaaggtcct
     3961 gacggacgac gaggagggag aggccgccaa ttcagagtcg gtcgaactgt cggacaaggc
     4021 ctcgcgcgaa ccggcgtcgc gcttcgagct gaagggtccc gtcaaggcca ctcccgatcg
     4081 cgccgagttg cctgggttcg atctcacgct ccatgcgaag ggtcacccgc agatcttcaa
     4141 gggcaagctc acgctcgact tcggcgagcg catcgaaggc gcggccgagc ttgcggcagg
     4201 ctttatcgat ctcgacgcgc tgttcggggt gccgggcgcc gaagagcggc cttcgcccgc
     4261 aaccgtcctt tatgagtttg ccgacgaggt cctcgcccag gcggcggcgc tcggcgacgg
     4321 cacactggca ttggttatcg aacaggcggg acttggcggc gatctggtcg gcgccatcaa
     4381 catgactctc gccgccaagg acggcgtcgt gaccatcggc caactcaagg caacgcttcc
     4441 cggagagaac cgcatcgagg cctcaggcag cctcaagcgc ggcgatttcg gcccggtctt
     4501 cgccgggccc gtcaaggtcg agggatccgg attgcggccg ctcacccgct gggccgcagg
     4561 cgatcgcgac gtgtcgggac aagcgtctgc cggcgacttc agcgtgatgg cgaatgccgt
     4621 cgtcggcgac ggcgcgctca tgctttccga tgcgtcgggc gagctgagcg ggacgaagtt
     4681 ccgcggggcc ttgcgcctta aggggggaga acgccggctg atcgagattg atctcgacag
     4741 cgaccggctc gatctgcgcg aggtgatcga cgagggaccg ctgtggcggt cttggctgac
     4801 cgcctctcaa cctgctgcgc cggcagcggc cagcaacgga gccacggccg caatcgacgg
     4861 gggcggcgcg accgggacgg aagatgccgc cgcgatgggg caggatctct tcaagcgcct
     4921 gcgtggcgac gacatgcgcg tgacgttgcg catcggcgag ctcctgctgc ccgacatccc
     4981 cgccggcaag ctcgatgcgc gctttgccct ccaaggcggc acgctcgccg tcgagcagct
     5041 cgatttcgcc gcggctgacg cgctcacgct caacgggaaa ggccgcatcg aacggctcga
     5101 cgaagcgccg tcgggcgggg tggattttgc ccttcgcgcc gcagatgcca gcagcctaaa
     5161 gattgccgcc gggttgttcg gcctgcccga gaaggtgagt ggctccgaac atctctcggt
     5221 cctcgctccc ctcgacctca acgtcaagct gaaggcgacc cgcgagggcg acttgaccaa
     5281 cgccgccgtc gagctcggcg gcaaggccgg cacatcggac atcgttctca ccgcgcgtgc
     5341 tctcggcgat cccgctaagc tcggtgaggc gaagatcgat gtctccgcca aggtcctcgg
     5401 cgaaaagccg caagcgttgc tcgtcctgct ctttcccgat ctgccgctcg agcgcatcgc
     5461 caccccggcc gggagcaagg gccggctgat cgtcaagttc gccggcgttc cgaacgccaa
     5521 ggtgaccggc aaggccgcgc tcgaaacgga gccgatcgag gtcgcctttg tcggccaggg
     5581 atcgctgcag ccaaacggcc tttcgcttga gggcaaggcc gcggcggtga gcaaggatgc
     5641 gagtgccgcg ctgacgctca tcggtttgga gtcgcccccg agcgccgcag gcgtccccct
     5701 cgcgcttcgc ctcgacgtcg caaagagcgg ggccacgatc gatcttaacg gcatcaccgg
     5761 gagcattgcg ggtgagaacg tcaccggctc cgtccaactg gaccggagcg gcgccaagac
     5821 gcgtttctcg ctgaagggaa gtgccggaag cgtgtcgctg ccgtcactgc tcggcgtgct
     5881 gatggcgtgg caccgcacgc cctcgaccga ggagatgctg ggcaccatca gcaccaatac
     5941 gtccgatgtt tggccatcgc gggggttctc gctttcgctg cttgaggagg tggaaggcgc
     6001 gatctcactc gacgcgaaga cgcttgccct cggttctgcc gtgaaggttg acgaggccac
     6061 tctcgccgct tccatcggca aggacgggct cgacgtcact gccctcacag gccgtctgtt
     6121 cgggggcgag cttgccgcat cgggcagcct cgcgccgcgc ggcaacggcg ccgagctgag
     6181 ggcgcgagcc gaggtgaaag ggggcaagct tgaagccctc gccaagagcc tgaccggatc
     6241 ggctctcgcc agaggaccct tcgaccttgc ctttaatgtt caaggcgagg ggttgagccc
     6301 tccaggcgtg gtggcgggac tgagcgggga ggggacgctc acccttggtc cgggcgccct
     6361 ccaatccttc tccgccgccc ccttgcgcgg cgtggccgca acggcggcca agaagagcat
     6421 caaggccgac aaggaggaaa ttgaggctga agcggagagc gtgcgtgaga ggatcaccaa
     6481 ggggacctac aaatttgcgc ctgtcgaact cgccttcgac atcaagaacg gcacgttgcg
     6541 tctcgctccg gcaacgctat cgagcgcagg cgccgaaacg aagatcaacg gtttcgtcga
     6601 gctcgcgagc ctcaagctcg acagcgaatg ggccatgggt ctgaccggag acggcgtcaa
     6661 ggatgtgccg ccggtgaacc tcgtcttcgc cggtgcgctg aacaaggcgg gcgcgatttc
     6721 gcccgcgatc gataccgggg ccatcgaggc ctatctcacc atgcggcgca tgcaagagga
     6781 cgttgagcgt cttgagactc tcgacgtgag cggcaggacc gcgcccgccg aggccgaacc
     6841 cgaggggtta gccataaccc taccggacga ttcgccggcc gaggcatcat ctccttccac
     6901 gccggaactt gccgaaccat cggcgtggtc cgccgagacc gagacgccgg aggagcccgc
     6961 ctcgcagcca tccgagacct cgacgacggc attgccgtcg gcgctcgagc ttcttgggac
     7021 gagcgaggag gctgcggtcg agaccgagac ccccgcaacg ggcgaacctg cagccttgtc
     7081 cgaacctcag gccgcgcccg aggcaccggc gtccgccacg cttggacccg cggcggaacc
     7141 ggcgccagcc ccggcggaag cggcaacggc gagccccgaa gcgcaaccaa gcccgcccgc
     7201 cgcggcgacc gaaaagccgc cccgcaagcg ttcggcaagc aagccccgcc ggcagaagca
     7261 agaagcgccc gatgcctgga ggaagaacgt cccgattttc ggcgggggtt agagccctcg
     7321 gcgcttcgtc cgctccagca cgtagagcct gatggctgcc gaaaggctca cagcctcgcg
     7381 gtccgtcgcg ctccttcgcc ggtcaatctc gcccacgatc gcggcaaccg acttgccttc
     7441 ggcgcgcgca atttcgccaa gcgcatccca gaagggctcc tccaccgtca cgctggtgcg
     7501 gtgccgcgcg atggtaagcg agcgcttttt gcgtgaaggc ggcatgcgcg aagcgggcgg
     7561 cctcaacgct tcgggccggt catgtcctct gggcgcacga gcctatcgaa gtcctcggcg
     7621 ttgacgtggc cgagccggat cgcctcttcg cgcaaggtgg tgccgcgctt atgcgcggac
     7681 ttggcgatct cgctcgcctt gtcgtagccg atatgcggcg cgagcgccgt gacgagcatc
     7741 agcgagcgct gcatgtgctc cttgacgcgc ggatgattgg cttcgatgcc gatcacgcaa
     7801 ttctcggtga agctatccgc gacgtcggcc agcaggcgga tcgactgaag cattgcgtag
     7861 gcgagcacag gcttgaacac gttcagctcg agatgcccct ggctgccggc aaaggcgatc
     7921 gtggcgttgt tgccgatgac ctgcgccgcc accatggtca ccgcctcgca ttgggttggg
     7981 ttgaccttcc cgggcatgat cgaggagccc ggctcgttct caggcagcag gagctccccg
     8041 aagcccgagc gggggccgga gcctagcagc ctgatatcgt tggcgatctt aaacagcccc
     8101 acggcgctgg cggtcagcgc cccatgcgcg aacacatagg cgtcgtggct cgcgagcgcc
     8161 tcgaacttat ttcgagctgt aacgaaagga agcttggtca tccccgcgat tttgctcgcg
     8221 aaaagctcgg cgaagcctgg gtgggtgttg agccccgtgc cgac
//
LOCUS       DASUQP010000133         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1562950, whole genome shotgun sequence.
ACCESSION   DASUQP010000133 DASUQP010000000
VERSION     DASUQP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562950"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..997)
                     /locus_tag="VFY74_05980"
     CDS             complement(80..997)
                     /locus_tag="VFY74_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5931283.1"
                     /translation="MESAPGVVGRIADILGRPVPPIDILDIGAMLEEGGDHYDPLLTQ
                     GLARVTGFEPNPAEFARLVNRPGPYRYLPVFLGDGGTAKFHLTRYPGCSSLLEPDPAV
                     INLFETMGCEEDGNFRVVRTEQVQTSRLDDLPADIKTDYIKIDIQGAELMVLQNGPRL
                     LAKTLVVETEAEFIPLYRGQPLFGDLQCFLRDYGFVLHKFVDFGGRPFRPLRPRNMFA
                     PISQAMWADAVFVRDFSRLDRYSDDDLIKAATILHVSILRSRGSLRARIRSRQAGGLW
                     RRYSSWLTTCDLPNAAVTFATQPTFARKA"
     gene            1187..1846
                     /locus_tag="VFY74_05985"
     CDS             1187..1846
                     /locus_tag="VFY74_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX5931284.1"
                     /translation="MRIWFLSLAFAFGLIGNLVGHSTQTFAALEDPGSCSLHSPAEGK
                     LRFARVNAPLPLSEPALPAIDNEGRDYLIRTLVFEAGGETEIGKAAVAHVILNRARLG
                     KWGDTVKEVVMRPWQFEPWMTRRGEMERMAPHEARYRDAARIADAVLLGEMPDPTAGA
                     THFLNPVIVRERRGGTLPKWARREGLPIGRHTFYALDGRLPAHAEHEPFLRFRPPPPS
                     C"
     gene            1988..2725
                     /locus_tag="VFY74_05990"
     CDS             1988..2725
                     /locus_tag="VFY74_05990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5931285.1"
                     /translation="MFPISDDNPRMGTPIVTWSIIGVCVLVFLWQLSLGEEQGQVAVF
                     QYGMIPARLFGAAELRPELAAVPAWSTVLTSMFMHGGVLHLALNMLFLWIFGDNVEDS
                     MGHLRYLVFYLVCGAAAAMTQALIHPDSTIPMVGASGAISGVLGAYLLLHPYATVRVL
                     IFIFLFVTIVHVPAMFVLGLWFLTQIASAAFAPADGPGVAFWAHVGGFVAGLALVPAF
                     RRNDVTLFQPAHSRPFAVERKARRPWG"
     gene            2848..3111
                     /locus_tag="VFY74_05995"
     CDS             2848..3111
                     /locus_tag="VFY74_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5931286.1"
                     /translation="MDIIAVLVTLAIGAIAGWLAGVLVEGAGFGLIGNILVGIAGAFI
                     AALLFPRLGLGLTLGGGLVGAIVTSALGAIVLLVIVNLLQRLF"
     gene            complement(3206..3676)
                     /locus_tag="VFY74_06000"
     CDS             complement(3206..3676)
                     /locus_tag="VFY74_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 domain-containing protein"
                     /protein_id="HEX5931287.1"
                     /translation="MQALLAAVEATSIAQYLRASRWGYAAVSFTHILGFALLIGAIVP
                     LNLRLLGLWPAIRRSTLMRVLVPAAAFGLMLAVPAGLLLFSVKAQDYSSIGFLQVKLA
                     LIGAGILSAVTAHLRYGLALEGASETRLRLHALISLGCWLGALCLGRLIAFALD"
     gene            complement(3680..4102)
                     /locus_tag="VFY74_06005"
     CDS             complement(3680..4102)
                     /locus_tag="VFY74_06005"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX5931288.1"
                     /translation="MAATVITVNIMSTVMDTLKTICLLSLLLALGLGLRPPASLAHHG
                     WAWTTGENMTLEGVIKEVKLGNPHGVLKVDSGGTLWTVEVGQPWRNERAGLKDGDLVA
                     GVTIRVIGEPASDSSDKRLKAERLFLGDREYVLYPDRD"
     gene            complement(4241..>4693)
                     /locus_tag="VFY74_06010"
     CDS             complement(4241..>4693)
                     /locus_tag="VFY74_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5931289.1"
                     /translation="VVIGDAGQDFDFARLNRAFRELIGGARFIALAKNRSFKDDDGAL
                     SLDAGAFVTALEFASGREALVLGKPSPAFFQAALASMDCRPESGVMVGDDAEADIAGA
                     LKAGLSAALLVRTGKYRAGDETRFSPRPTATVADLSAAARWIIAQRAP"
BASE COUNT          830 a         1470 c         1510 g          883 t
ORIGIN      
        1 gaagatgcgg gtaaaaaagc tgacgacggg atcgagcatg gcgacactat agcttcggtc
       61 gccaagtctg tcttgccgtt caggccttgc gcgcgaatgt gggctgggtg gcgaatgtca
      121 ctgccgcgtt cggaaggtcg cacgtcgtca accaggatga atagcgtcgc cacagcccgc
      181 cggcctggcg cgatcgaatt cgcgcacgta aactcccgcg agatcgtagg atcgagacat
      241 ggaggatcgt tgcggctttg atcaggtcgt cgtcgctgta gcgatcgagg cgcgaaaagt
      301 cgcgcacgaa gacggcatcc gcccacatgg cctggctgat cggcgcaaac atgtttctcg
      361 ggcgcaacgg gcggaacggg cgcccgccga aatcgacaaa cttgtgcaag acgaaaccat
      421 aatcgcgcag gaagcattgc aggtcgccga acaggggttg gccgcgatag agcgggatga
      481 actcggcttc ggtttccacg accagagtct tggcgagcag gcgcgggccg ttttgcaaaa
      541 ccatgagttc ggcgccctgg atgtcgatct tgatatagtc cgttttgatg tcggccggca
      601 gatcgtcgag ccgcgatgtc tgcacctgct ccgtcctgac gacgcgaaag ttcccgtctt
      661 cctcgcagcc catcgtctcg aacagattga tgacggcggg atcgggctca agcagggagg
      721 aacagcccgg atagcgggtg agatggaatt ttgcggtccc gccatcgccg agaaacacag
      781 gcaggtagcg ataaggtccg ggacggttga cgagccgggc gaactcggcc gggttcggct
      841 caaagcccgt gacccgcgcc aagccctgcg tcagcaaagg gtcgtaatga tctcccccct
      901 cctccagcat ggcgccgatg tcgaggatat cgatcggcgg gacaggacgg ccgagaatat
      961 cggcaatcct cccaacaacg cccggcgcgc tttccatgga cgtatcactg tgccgtggcc
     1021 gggcggcggc gcttgttgag cacaccgatc gccgcgccta gacctgtaaa ggcttgccgc
     1081 aattgttccc aagttcgcta ccgtatcgtc ataaaatgat cgcctttttc cagcgaatcc
     1141 gcacccttcg acccgatcca attgcagtaa ttgggggaga gttgcgttgc ggatttggtt
     1201 cttgtccttg gcctttgcgt ttggcctcat cggaaacctc gtgggacatt cgacgcagac
     1261 tttcgcggcg ctcgaagatc ccggaagttg ttcgctgcat tcaccggcgg aaggcaaatt
     1321 gcgatttgcc cgcgtcaatg cgcccctccc cttgtccgag ccggcgcttc cggcgatcga
     1381 caacgaagga cgggattacc tcattcgaac gctcgtcttt gaagcggggg gcgagaccga
     1441 gatcggcaag gcggcggtgg cgcatgtcat cctcaatcgc gcgcgcctcg gcaaatgggg
     1501 cgataccgtc aaggaagtgg tcatgcgtcc ctggcagttc gagccgtgga tgacgcggcg
     1561 gggcgagatg gagcgcatgg cgccgcacga ggcgcgctac cgcgatgcgg cgcgcatcgc
     1621 cgacgccgtt ctcctcggcg agatgcccga tcccacggcc ggcgccacgc atttcctgaa
     1681 cccggtgatc gtgcgggaga ggcgcggcgg gaccttgccc aaatgggcgc gccgcgaagg
     1741 gctcccgatc ggcaggcata cgttctatgc gctcgacggc agattgcctg ctcatgccga
     1801 gcatgagccc tttttgcgct tccgcccccc tccgccttcg tgttgaggcg ttcgattcgc
     1861 gtcgcaccaa aagcccgcgc cagcgcgcga tgagaggggt ggccggtatc gccagccctc
     1921 ctcactcggc ggcggcatca tgtcttgcgg aacttttgcc gagcgatcat agtcccgccc
     1981 gcaacgcatg tttcccatct ccgacgacaa tccgcgcatg gggacgccga tcgtcacctg
     2041 gtcgatcatc ggcgtctgcg tactggtatt tctttggcaa ctctccctcg gggaagagca
     2101 agggcaagtc gcagtctttc agtacgggat gatccccgcg cgcctgtttg gtgcggccga
     2161 attacgcccc gagcttgccg ccgtccccgc ctggtccact gttctcacct ccatgttcat
     2221 gcatggaggc gttctgcatc ttgcgctgaa catgctgttc ctgtggatct tcggcgacaa
     2281 tgtcgaggac tccatgggcc atcttcgcta tctcgtcttt tatcttgtct gcggcgccgc
     2341 cgcggcgatg acccaggccc tgattcatcc cgattcgacc ataccgatgg tcggcgctag
     2401 cggcgccatt tccggcgttc tcggtgccta tcttctcctg cacccctacg ccaccgtgcg
     2461 cgtcttgatc ttcatcttcc tgttcgtcac catcgtgcat gtgccggcga tgttcgtgct
     2521 cggtctttgg ttcctgacgc agatcgccag cgcggcattc gcgccggctg acggaccggg
     2581 cgttgccttc tgggcgcatg tcgggggatt cgtcgcaggc ctggcgcttg tgccggcctt
     2641 caggcgaaac gacgtgacgc tgtttcagcc ggcgcattca cgccccttcg ccgtcgagcg
     2701 caaggcgcgc cgcccttggg gctgacgggc tcgcgggcct cgcaggggcg cgttccgaag
     2761 cggctggtgc cgcttgccgc aatgcgctaa atcttggggg tctcgttcgc gccgagcgcg
     2821 cgggaccaaa cttaaggggg gtaaatcatg gacattattg cagtgcttgt gactctggcg
     2881 attggtgcga tcgctggctg gcttgcaggc gtcttggttg agggtgctgg gttcggcctt
     2941 attggcaata ttcttgtcgg catcgccggc gcctttatcg ccgccttgct gtttccgagg
     3001 ctcggtctcg gcctcactct gggcggaggc cttgtcggcg ccattgtgac ctcggccctt
     3061 ggcgcgatcg ttctgctcgt gatcgtcaat ctgctgcagc ggttgttcta ggcatcacgt
     3121 tcgccgaaac tttcgtggcc ggacaggcgc gccggatcgg cgcgcctgac accggccatg
     3181 acgcgcggtg atcaagcgcg ggggctcagt ccagagcgaa ggcgatgagg cgccccaggc
     3241 aaagcgcgcc gagccagcag ccaagcgaga tgagtgcatg gagcctgagc cgcgtctcgc
     3301 ttgcaccttc cagcgccagt ccatagcgaa gatgcgccgt taccgccgat aggatgccgg
     3361 ccccgatcag agcgagcttc acctggagaa agcctataga gctgtaatcc tgcgccttga
     3421 ccgagaacag gagaaggccc gcgggcacgg cgagcatgag gccaaaggcc gccgcaggga
     3481 cgagcacgcg catgagggtt gagcgccgga tcgccggcca cagcccgagc agacgcaagt
     3541 tgagcggcac gatcgcgccg atgaggagag cgaagccgag gatgtgcgtg aagcttaccg
     3601 cagcgtagcc ccagcgcgag gcgcgcagat actgcgcgat gctggtcgct tcgaccgccg
     3661 cgagcaaagc ctgcatggcc tagtcgcgat cggggtagag cacgtactcg cggtcgccga
     3721 ggaacagccg ctcggccttg agccgcttgt ccgatgagtc cgaagcaggc tcgccgatca
     3781 cacggatggt gacgcccgcg acgagatccc cgtccttgag ccccgcgcgc tcgttgcgcc
     3841 aaggctggcc gacttcgacc gtccacagcg tgccgccgga atcgactttg agaacgccgt
     3901 gcggattgcc gagcttcacc tccttgatga cgccctcgag cgtcatgttc tcgccggtgg
     3961 tccaggccca gccgtgatgg gcgagagaag caggcgggcg aaggccaaga ccaagcgcga
     4021 gaagcagaga gagcaggcag atcgtcttga gcgtatccat gacggtactc atgatgttca
     4081 ccgtgatcac agttgcagcc aaatgcgttc atctttgaga tggtgacgaa acgccgggat
     4141 gcgcaagacc gtttcccgca atgcaccaat accctcacct tgaggagggg gccgaaggcc
     4201 cgtctcgaag ggttagacga cagtctgatc ggcgcgaggc tcaaggcgca cgctgcgcga
     4261 tgatccaacg cgcggcggcg gacagatcgg cgaccgtggc cgtgggccgg ggggaaaacc
     4321 gcgtctcatc acccgcgcgg tatttcccag tgcgcaccag cagggcggcc gagagtcccg
     4381 ctttcagcgc gccggcgata tcggcctcgg cgtcgtcgcc gaccatcacg ccactctccg
     4441 gccggcagtc catgcttgcg agcgccgcct ggaagaacgc cggcgaaggt ttccctaaga
     4501 ccagcgcctc gcgcccgctt gcgaactcga gcgcggtgac gaaagccccg gcatcgaggc
     4561 tcaaggcccc gtcgtcgtcc ttgaaggagc ggttcttcgc cagcgcgatg aagcgagcgc
     4621 cgccgatcaa ctcccggaac gcacgattga ggcgggcgaa gtcgaaatcc tgtccggcgt
     4681 cgccgatcac gac
//
LOCUS       DASUQP010000134         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1562996, whole genome shotgun sequence.
ACCESSION   DASUQP010000134 DASUQP010000000
VERSION     DASUQP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562996"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFY74_06015"
     CDS             complement(<1..210)
                     /locus_tag="VFY74_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX5931290.1"
                     /translation="MFETLSERLGAILDKLTRKGALAEADVTEAMREVRRALLEADVA
                     LDVVRDFTEAVKAKAVGQEVVRSVTP"
     gene            complement(515..1942)
                     /locus_tag="VFY74_06020"
     CDS             complement(515..1942)
                     /locus_tag="VFY74_06020"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX5931291.1"
                     /translation="MPGRSSFRYGKGFRSLTAETREPVRLAVSGTLPAWLEGTLLRTG
                     PAKFEVGARTLNHWFDGFAMLYRFAIADGGVTYANRFLKSKAFSAAEETGKISYSEFA
                     TDPCRTLFGRVAAVFAPKPTDNCNVNVSDYARETVAFTETLMPIRFSPETLETLGVFA
                     YGEALGGQLSTAHPHYDAKRACHYNYVTAFGMESLYRICRIGDDGAQQQIAEIAVERP
                     SYMHSFGMSEDHLILAEFPLVVSPIDLRMSGKPFIRNYRWEPERGIVFHVVAKDSGEI
                     VRTGKTDATFAFHHVNAFADDGGLAVDVVAYPDASIIDQLYLSRLRANAPITATGRLV
                     RFQVPLADGAPITRKILAAAPLEVPRINYERCAGKPYRLVWGAGIEVEGDFLDSVVKI
                     DTKTGTVATWHQAGLYPGEPVFVPSPAAKAEDEGVLLSVVIDIVKDRSFLLVLDAASL
                     AELARADAPHAIPFHFHGAFFASTA"
     gene            complement(1978..2307)
                     /locus_tag="VFY74_06025"
     CDS             complement(1978..2307)
                     /locus_tag="VFY74_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX5931292.1"
                     /translation="MPYLYLAAAITFEVIGTSALKASDTFTRLVPSLVTLAAYAVSFL
                     FLALSLRTIPVGIAYAMWAGLGIVLIALVGWLWFKQPLDGPAIIGLALIIGGVVVVNA
                     FSQSLPH"
BASE COUNT          479 a          703 c          788 g          419 t
ORIGIN      
        1 gggagtgacc gaacgcacca cctcctggcc gaccgctttc gctttcacgg cctcggtgaa
       61 gtcgcggacc acgtcgagcg cgacatcggc ttcgagcagc gcgcggcgca cctcgcgcat
      121 ggcctcggtc acgtcggcct cggccagagc ccctttgcgg gtaagcttgt cgaggatggc
      181 gccgaggcgt tccgataagg tctcgaacat cgtctctcct tcttcctcat gctgaggagc
      241 ggcgaagccg cgtctcgaag cagaggtcac ccttcgagac gggccttcgg ccctcctcag
      301 ggtgaggtat gtcaaaacat caaacgcacc cgagggcgca tcgcgctgtc gggtgttgac
      361 ccccctgcct ccttattttc gcgctaccgc gctttgcgct acttgagcgc gatgggcgat
      421 agccagtagc gcaaaaatca tgtgcagggc ggcggctcgc gttacggagc ggccttgaat
      481 ttcgccggga atgtcggcga ggccgcgcct taagtcaagc cgtgctcgcg aagaaggcgc
      541 cgtggaaatg gaagggaatg gcgtgagggg cgtcggcccg cgccaattct gccaggctcg
      601 ccgcatcgag cacgagaagg aaagagcggt ccttgacgat atcgatcacg acggacagca
      661 agacgccctc atcctcggcc ttggcggcag gcgagggcac gaataccggc tcgccgggat
      721 agaggcccgc ctggtgccag gtggcgaccg tcccggtttt cgtgtcgatc ttgacgacgg
      781 agtcgaggaa atcgccttcg acttcgatgc ctgcgcccca aacaaggcga tagggcttgc
      841 cagcgcatcg ctcgtagttg atgcgcggca cctcaagcgg tgcggcggcc aagatctttc
      901 gcgtgatggg cgccccgtct gccagcggaa cctgaaagcg gacgagcctg ccggtggcgg
      961 tgatcggcgc gttggcgcga agccgcgaaa ggtaaagctg atcgatgatc gacgcgtcgg
     1021 gatacgcgac cacatcgacc gcaaggccgc cgtcatcggc gaaggcattg acgtgatgga
     1081 aggcgaacgt cgcatcggtc tttccggtgc gcacgatctc gccgctgtcc ttggcgacga
     1141 catggaagac gatgcctcgc tcaggctccc agcgatagtt ccgaataaaa ggcttgcctg
     1201 acatcctgag atcgatcggg ctcacgacca gcgggaactc ggccaggatc agatggtcct
     1261 cgctcatgcc gaagctgtgc atgtaggagg gccgctcgac ggcaatctcg gcaatttgtt
     1321 gttgagcgcc gtcatcgccg atgcggcaaa tgcgatagag gctctccatg ccgaaagccg
     1381 tcacgtagtt atagtggcac gcgcgcttcg cgtcgtaatg cggatgcgcg gtcgagagct
     1441 ggccgccaag cgcttcgcca taggcgaaga cgccgagtgt ctcgagggtc tcgggcgaaa
     1501 agcgaattgg catcagcgtc tcggtgaagg cgaccgtctc gcgcgcgtag tccgagacat
     1561 tgacgttgca attgtcggtc ggcttcggcg cgaacaccgc agctacacgg ccgaacagcg
     1621 tgcggcacgg gtcggtcgcg aattcgctgt aggagatctt gccggtttct tccgcggcgg
     1681 agaacgcctt gctcttgagg aaacggttgg cgtaggtgac gccgccatcg gcaattgcga
     1741 agcggtacag catcgcaaat ccgtcgaacc aatgattgag ggtgcgcgcg ccgacctcga
     1801 acttggcggg cccggtgcgg aggagcgtgc cttcgagcca ggctggaagc gtgccgctga
     1861 ccgcgagccg aaccggctcg cgcgtctcgg ccgtcaggga gcggaaccct ttgccatagc
     1921 gaaaggacga tcggcccggc atcacgcccc tcaaacaacg gaattcagtt cattccgcta
     1981 gtgcggaagc gactgtgaga acgcatttac gacgaccacc ccgccgataa taagggcaag
     2041 cccaatgatt gccggcccat cgagcggctg tttgaaccaa agccatccga ccagggcaat
     2101 gagcacgatg ccaaggccgg cccacatggc gtaggcgatg ccgaccggga ttgttcttag
     2161 gctgagcgcg aggaacagga aggacacagc ataggcggcc aaggtcacga ggctcggaac
     2221 aagacgtgtg aaggtgtcgg acgccttcag ggccgacgtc ccgatgacct cgaatgtgat
     2281 cgcggcggca aggtagagat aaggcaaagc gagagtcaca gatgaactcc ttggtttgcg
     2341 gaacttgctt gcgggaatgt gttggcaatt gtgacggcga gatctaaca
//
LOCUS       DASUQP010000135         8166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_474072,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000135 DASUQP010000000
VERSION     DASUQP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474072"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="VFY74_06030"
     CDS             complement(<1..2022)
                     /locus_tag="VFY74_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5931293.1"
                     /translation="MHPRADGWRDDPYIPLIMAITKSRTAAPAGREVARGDHVYLIDG
                     SGYIFRAYHALPPLTRPSDGLPVGAVHGFCAMLWKLLRETGELAPPTHIAVVFDYSAK
                     TFRSDLFDGYKANRPEPPDDLIPQFPLVRDAVRAFNVACIEQEGYEADDIIATYALQA
                     MAAGAEVTIVSSDKDLMQLVQPGIRMYDTMKNKVIGEAEVEERFGVPPSKVVEVQALI
                     GDSSDNVPGVPGIGVKTAALLINEFGDLETLLGRASEIKQDKRRENLISFADQARLSK
                     SLVILDTHVPLEVALSETAVRQPDTAALTSFMRKLEFTTLLRRVAEGLGAPSPLAGEG
                     GRERSDRPGEAEPLASKGRAGPKLAYDHPLRRTPRGETGPPRERKVEGGSPAQLAVER
                     ASRLEAMPFDRANYVMVTDATRLADLIEAARYQGHFAFRVKLNGPDPMRGEIAGVVLA
                     LVPGRAAYVPLAHRAGDGLDLGGEAIPQIPLREALDLLKPILEDASVLKIVQNAKFDM
                     VALARYGIALASIDDPCLMSYALDAGRAEHLPEELAGRLLGYTCLTEKEVLGSGRNAV
                     AFDRVALARATEYAGEETDIGLRLWMILKPRLAAESVTTVYETLERPLAPVLANMERH
                     GVKVERGTLSSLSLSFAQTIARLEDEIRELAGEDFNIGSPKQLGEILFDK"
     gene            complement(2329..4002)
                     /locus_tag="VFY74_06035"
     CDS             complement(2329..4002)
                     /locus_tag="VFY74_06035"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5931294.1"
                     /translation="MAQAADRKKVLVIGGGFGGVFAAKALERLGRGTLEVELINRNNY
                     FVFQPLLPEVAASTIHSADAVVPLRQLLRRLQVRLAEVMGIDFAKKTVTVVQGTPRIP
                     VDLAYDELVIALGTGVDLKRFPGLPEHALTMKDLADAHRLRTHVIGCLETADVTDDPE
                     VKRQLLTFVVVGAGFSGVETVAEVRELVRYALKYYPNIRPEEIRFYLIEYANRILPTF
                     PADLAAYATRQLDKSGIEVLTGVGTKTATGTAAELTDGRVISTSTIVATIGNGPHMLV
                     EALGLDLKWGRIKTDRFMRVPGLDGVWALGDSALIPLVDTPQEDPAHYAPQTAQFAVR
                     EGRQLAANVVAKIAGLDLKPFAYTSKGSLASLGMSKAVADVYGIKLSGLLAWLLWRGF
                     YLSFLPGFAAKFRVGLSWLLNSVMPPNIVQIRSTPPATRYLYYRKGDRVFEPGMLIDG
                     FYTVTKGAFRLTIDNPETGEHFEKVFRTGDHFGERLLLRSALRTGLVVALEDSILLYI
                     AQKDFTRLARAFPVLDSYFKDYIERTFGGADKAFAPGSTEQKTELETLP"
     gene            complement(4073..4984)
                     /locus_tag="VFY74_06040"
     CDS             complement(4073..4984)
                     /locus_tag="VFY74_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018070933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5931295.1"
                     /translation="MSKSLEGRIALVAGATRGAGRGIAAELGAAGATVYVTGRSTRTQ
                     KSEMNRPETIEETAELVDRNGGKGIPIRVDHLKPDEVRALVERIKRDHGALDILVNDI
                     WGATKMEWNKSVWESDLEYGLKTLRLAVDTHAITSHFALPLLIEKRGGLVVELTDGTS
                     EYNATNYRVSFFYDLAKAAVNRMAFSLAHELQPHGATAVALTPGWLRSEVMLDAYRVT
                     EANWRDATKISPHFAISESPAFAGRAVVALASDPDAMRWSGQVLSSGQLSRHYGFTDI
                     DGSQPDAWRYVPEVRDTGKPADTSGYR"
     gene            5419..5495
                     /locus_tag="VFY74_06045"
     tRNA            5419..5495
                     /locus_tag="VFY74_06045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5453..5455,aa:Arg,seq:ccg)
     gene            5590..5781
                     /locus_tag="VFY74_06050"
     CDS             5590..5781
                     /locus_tag="VFY74_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931296.1"
                     /translation="MRKVLLVAAAAAIALVGLSANTCGGSEEKAAEPPAPAAAPEPPA
                     AAPEPATEPAEPPATPPPQ"
     gene            complement(5877..6344)
                     /gene="bcp"
                     /locus_tag="VFY74_06055"
     CDS             complement(5877..6344)
                     /gene="bcp"
                     /locus_tag="VFY74_06055"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX5931297.1"
                     /translation="MSVEVGDKAPDFSLPSDNGGKASLKALGGKTVVLYFYPKDDTSG
                     CTAEACAFRDTLPDFSKVKAEILGISRDSVASHAKFKTKFDLAFPLASDEDGKVCEAY
                     GVWVEKSMYGRKYMGIERSTFLIDGKGVIRNVWRKVKVDGHAEEVLKAAAAIK"
     gene            complement(6381..7193)
                     /locus_tag="VFY74_06060"
     CDS             complement(6381..7193)
                     /locus_tag="VFY74_06060"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5931298.1"
                     /translation="MRHTLLSVLVLGWLGSLCALSPSPASSQEIDLSASRVIDLTHPF
                     DKDTVYWPTSPSGFELKPLHKGLTAGGFYYSAYAFCAPEHGGTHLDAPVHFAEGVWSG
                     SDIPVERFIGPAFVIDVSEKAKENRDYTLTVEDITKFEAEHGKIASGAIVLLRTGWGS
                     RWPDRLAYLGDNKPGDASNLHFPSYGPEAVEFLIRERRVGTIGVDTASIDPGTSQDFP
                     VHRLAAANNVSGLENLANLNELPPVGATVIALPMKIGGGSGGPVRAIALAPK"
     gene            complement(7241..>8166)
                     /locus_tag="VFY74_06065"
     CDS             complement(7241..>8166)
                     /locus_tag="VFY74_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HEX5931299.1"
                     /translation="SGLSSGAFMAVQIQVAHASDIAGAGIVAGGPFACAESAASRAFP
                     FWPIAVTQNTAQALNKCMKTDWGEPDPALSVERAKELADAGAIDPIAGLKPDNVYLFS
                     GNEDETVTRPVVRAAAEFYKKAGVDPGNISLVEKEGGHAFLTEQGGAACGISEAPFVS
                     DCNYNQAQAILAWIYGPLSAPSPEPSGRFIVFDQGDFSEPGDGFADEGVVYVPKACAD
                     RPGCRIHIALHGCDQSREEIGDTFIKDSGFAEIADQNRLIVLFPQVKASTVNRHGCWD
                     WWGYTGLDYLGKDAPQIKAIWAMAEKLAAAP"
BASE COUNT         1398 a         2585 c         2714 g         1469 t
ORIGIN      
        1 cttgtcgaac aggatctcgc cgagctgctt cggcgagccg atattgaaat cctcgccggc
       61 aagctccctg atctcgtcct cgagccgcgc gatggtctgg gcgaaagaga gcgacaggct
      121 cgacagcgtc ccccgctcga ccttcacccc gtggcgctcc atgtttgcga gaaccggcgc
      181 gagcggccgc tccagcgtct cgtagacggt ggtgacggac tccgccgcaa gccgcggctt
      241 aaggatcatc cagagccgaa gcccgatgtc ggtttcttcg cccgcatatt cggtggcccg
      301 cgcaagcgcg acccgatcga acgcgacggc gttgcggccc gaaccgagca cctccttctc
      361 ggtgaggcag gtatagccaa ggagccggcc tgcgagctcc tcgggcagat gctcggcgcg
      421 gcccgcgtcg agcgcgtagg acatgaggca gggatcgtcg atgctcgcca gcgcgatgcc
      481 atagcgcgcg agcgcaacca tgtcgaattt cgcgttctgc acgatcttca gcacgctcgc
      541 gtcttccaag atcggcttca ggagatcgag cgcctcgcgc agtggaatct gtgggatggc
      601 ctcgccgccg agatcgagcc cgtcgccggc gcggtgcgca agcggcacgt aggcggcgcg
      661 acccggcaca agcgcgagca cgacgccggc gatctcgccg cgcatcggat caggcccatt
      721 gagcttcacc ctgaaagcga aatggccctg gtagcgggcg gcctcgatca ggtccgcgag
      781 ccgcgtcgcg tccgtcacca tcacgtaatt ggcgcgatcg aacggcatgg cttcgagacg
      841 ggaggcgcgc tcgacggcga gctgcgcggg cgagccgccc tcgacctttc gctcgcgcgg
      901 cgggcccgtc tcgccgcggg gcgtgcgccg caggggatgg tcgtaagcga gtttcggtcc
      961 agctctcccc ttgctcgcga gtggctccgc ctcacccggc cggtcgcttc gctcccggcc
     1021 accctctccc gcgaggggag agggagcgcc aagcccttcg gccacgcggc gaaggagcgt
     1081 cgtgaattcg agcttccgca tgaagcttgt gagcgcggcg gtgtcgggct ggcgaacggc
     1141 ggtttcggag agcgccactt cgagcggcac gtgagtgtcg aggatcacga gcgatttcga
     1201 cagccgcgcc tggtcggcga agctgatgag attctcgcgc cgcttgtcct gcttgatctc
     1261 ggacgcgcgg ccgagaagcg tctcgagatc gccgaactcg ttgatgagga gcgccgcggt
     1321 cttcaccccg atcccgggaa cgcccggaac gttgtcggag gaatcgccga tgagcgcctg
     1381 cacttcgacc accttcgacg gcggcacgcc gaagcgttcc tccacctcgg cctcgccgat
     1441 caccttgttc ttcatggtgt cgtacatgcg gatgccgggc tgcacgagtt gcatcaggtc
     1501 cttatcggag gagacgatgg tcacctctgc gccggctgcc attgcctgaa gcgcataggt
     1561 ggcgatgatg tcgtcggcct cgtatccctc ctgctcgatg caggcgacgt tgaaggcgcg
     1621 taccgcatcg cgcacgagcg ggaattgggg aatgagatcg tccggcggct cgggccggtt
     1681 cgccttgtaa ccgtcgaaca ggtcgctgcg gaatgtcttg gcggaatagt cgaagacgac
     1741 cgcgatatgg gtgggcgggg caagctcccc cgtctcccgg agcagcttcc acagcatggc
     1801 gcagaagccg tgcacggcgc cgacgggcag cccgtcggag gggcgcgtta gcggcgggag
     1861 cgcgtggtag gcgcggaaga tgtagcccga accgtcgatc agatagacgt gatcgccccg
     1921 cgcgacctcc ctgccggcgg gggcggcggt cctgctcttc gtgatggcca tgatgagggg
     1981 aatataggga tcgtcgcgcc acccgtcagc acggggatgc acctatcccc agccgtcatg
     2041 cccggacacc cgggtccggc ttcgccggcc cgggcgcagg cttgtccggg catccatgcc
     2101 gtttcgtttc cgccagatga agcgcaatgg aatggattgc cgggccttcg cccgccgaag
     2161 cgatgcctga cgagcgtgac cacgaggctt cggcccgcgt aggcgggtca agcccggcaa
     2221 tgacgaagat aagcggggct agctcgtcct gctgcctgct gcgtcctcat gctgaggagg
     2281 gccgaaggcc cgtctcgaag cacggacagg gggccgccgg tatggcgttc agggaagcgt
     2341 ctcgagctcg gttttttgct cggtgctgcc gggcgcgaag gctttgtcgg cgccgccgaa
     2401 cgtccgctcg atatagtcct tgaaatagga gtcgagcacg gggaaggcgc gggcgagccg
     2461 cgtaaaatcc ttctgcgcga tgtagaggag gatgctgtcc tcgagcgcga cgacaagccc
     2521 ggtgcggagc gccgagcgga gcaagaggcg ttcgccgaaa tgatcccccg tacggaacac
     2581 cttctcgaaa tgctcgccgg tctcgggatt gtcgatggtg agccggaacg cgcccttggt
     2641 cacggtatag aagccgtcga tcagcatgcc gggctcgaac accctgtcgc ccttgcggta
     2701 gtagaggtag cgcgtcgccg gcggtgtcga ccggatctgc acgatgttgg gcggcatgac
     2761 gctgttgagg agccagctca agccgacgcg gaacttggcg gcgaagcccg gcaagaaaga
     2821 caggtagaag ccgcgccaca agagccaagc gagcaagccc gacagcttga tgccgtagac
     2881 atcggccacc gccttgctca tgccgagcga ggcaagcgat cctttcgagg tgtaggcgaa
     2941 cggcttgagg tccaagcccg cgatcttggc gacgacattg gcggccagct gccgtccctc
     3001 gcgcacggcg aactgcgccg tctgcggcgc gtaatgggcc gggtcctctt gcggcgtatc
     3061 gacaagaggg atgagcgccg agtcgcctag cgcccagacg ccgtcgagac cgggcacgcg
     3121 catgaagcga tcggtcttga tccgccccca tttcaagtcg aggccgagcg cctcgacaag
     3181 catgtgcggc ccgttgccga tggtggcgac gatggtcgag gtcgagatca cgcgcccgtc
     3241 ggtcagctcg gccgccgtcc ccgtcgccgt cttggtgccc actccggtca agacctcgat
     3301 gccgctctta tccagctgcc gggtggcgta tgcggcaaga tcggcgggaa aggtcggcag
     3361 gatcctgttc gcgtattcga tcaggtagaa gcgaatctcc tcgggtcgga tgttcgggta
     3421 gtacttcaac gcgtagcgga cgagctctcg cacctccgcg acggtctcga cgccggagaa
     3481 gccggccccg acaaccacga aggtgaggag ctggcgcttg acctcgggat cgtcggtcac
     3541 atccgccgtc tccaagcagc cgatgacgtg ggtccggagc cggtgggcgt ccgcgagatc
     3601 cttcatggtg agcgcgtgct cgggcagtcc ggggaagcgc ttgagatcga cgccggtgcc
     3661 gagcgcaatc acgagctcgt cgtacgcaag atcgacgggg atgcgcgggg tgccctgcac
     3721 cacggtcacg gtcttcttgg cgaagtcgat ccccatgacc tcggcgagcc ggacctggag
     3781 ccggcgcagg agctggcgaa ggggcacgac ggcgtcagcc gaatgaatgg tcgaggcggc
     3841 aacctcgggc agcagcggct ggaacacgaa gtagttgttg cggttgatga gttcgacctc
     3901 gagcgtgccc cggcccaggc gctcgagcgc tttggcggcg aacacgccgc cgaaaccgcc
     3961 gccgatcacg agcaccttct tcctgtcagc cgcttgcgcc atgggtttcg ttcaccttgt
     4021 ggctttcagc cccataccta cgccgatggg attgcgcgtc aagccgcgtc gatcagcggt
     4081 aaccggacgt gtcagcgggc tttcccgtgt cccgcacttc gggcacgtag cgccaagcat
     4141 cgggctgact gccgtctatg tcagtgaacc catagtgacg cgacagctgg ccgctcgaaa
     4201 ggacctggcc ggaccagcgc atcgcatcgg gatcgcttgc gagtgccacg actgcgcggc
     4261 cagcgaaggc cggactttcg gagatggcga agtgcggcga gattttcgtc gcatcacgcc
     4321 agttggcctc cgtgacacga taggcatcca acatcacttc cgatcgcagc cagccgggcg
     4381 tcagtgcgac ggcagtcgca ccgtggggtt gaagctcatg cgcgagagag aaagccatcc
     4441 ggttcactgc cgccttggcc aagtcataaa agaaagaaac ccggtagttg gtcgcgttgt
     4501 attcactcgt tccgtcggtg agttcgacca ccaatccgcc cctcttttca atgagcagcg
     4561 gaagcgcgaa gtggctcgtg atcgcatgcg tgtcgaccgc aaggcgaagc gtcttgagcc
     4621 cgtactccag gtcagactcc cagacggact tgttccactc catcttcgta gcaccccaga
     4681 tgtcgttgac gaggatatcg agcgctccgt ggtcgcgctt tatgcgctcg accagcgccc
     4741 ggacttcgtc cggcttcagg tgatcgacgc ggatcggaat gcctttgccg ccgttgcgat
     4801 cgaccaattc ggccgtctct tcgatcgtct ccggccggtt catctcggac ttttgcgtgc
     4861 gcgtggaacg gccagttaca tagactgtcg cgcccgctgc gccgagttcg gcggcaatcc
     4921 cccggcctgc gcctcgcgtg gcgccggcga ccaatgcgat tcttccctca agtgactttg
     4981 acatggtgag ccctcatttc gagcggactt gcaatatgtt tattaccgcg taggcgcggg
     5041 gcgcgctcgc tccgcgccga tcacaaccat ggtgtcggcc gaccggtcat ggaccacctc
     5101 gtgccctccg tgccaagcgc ggcgaatctc gcgaggagat ctgtccggtc cgtcacgcgc
     5161 gggccagcct cggcgggcgc ctggaactga acgagagcga ctgtcaccag cgggcaagat
     5221 caaccaggac aggttcattc ccaagccttt gcttggcttc gtgacttctt taggtgccat
     5281 ccgggggctc acttggcgcc ttgcccgagg aacggaaagg gagccttaac cgttgttttc
     5341 cgcgcaggag tctgatttgt ggacggggcc agccgccgtt gcttcaaaca taccctttgg
     5401 ctaggttcgg gcggcgaggc acccgtagct cagttggata gagcgttgcc ctccgaaggc
     5461 agaggtcaca ggttcgaatc ctgtcgggtg cgccaaatgt ggcagcaaaa agcctgaaac
     5521 tggcgtataa gctgggatcg ctgagggctt caagggtcgt aacctgaact taggaatgga
     5581 gagaatcaca tgaggaaagt tcttctggtc gcagcggccg cggcgatcgc gctcgtcggt
     5641 ttgtcggcca acacctgcgg cggtagcgag gaaaaggcgg ccgagccgcc ggcgcccgcg
     5701 gcggctcctg agccccccgc ggcggcgcct gagccagcga ccgagccagc cgagccgcct
     5761 gcaacgcctc cgccgcagta atttaagcta aagggtggcc gcccctgttg cggcctccgt
     5821 tacgggacgg ctccggccag tgcttctggc cggcgccgtc ctttttgttg agagacctat
     5881 ttgatcgccg ccgcggcctt gagcacctct tcggcatgcc cgtcgacctt gactttgcgc
     5941 cacacgttgc ggatgacgcc cttcccgtcg atgaggaagg tcgaccgctc gatgcccatg
     6001 tacttcctgc cgtacatgct cttttcaacc cagacgccgt aggcctcgca gaccttgccg
     6061 tcttcgtcgg aggcaagcgg aaaggcgagg tcgaacttgg tcttgaactt ggcgtggctc
     6121 gcgacggagt cgcgtgagat ccccaagatt tcggccttca ccttcgagaa atcgggaaga
     6181 gtgtcgcgga aggcgcaagc ctctgccgtg cagccagacg tatcgtcctt cggatagaaa
     6241 tagagcacga ccgtcttgcc cccgagcgcc ttcagcgatg ccttgccgcc attgtccgac
     6301 ggcaggctga aatccggcgc cttgtctccg acctcgacgc tcatcgcccg cctccttgct
     6361 tctccaattg cgtggcttcg ctatttcggc gcgagcgcga tggcgcgcac agggccgccg
     6421 gagccgcctc caattttcat cggcagcgcg atgacggtcg caccgacggg cggcagctca
     6481 ttcaggttgg cgaggttctc gagccccgaa acgttgttcg ccgcagcgag ccgatgcacg
     6541 ggaaagtctt gcgaggtgcc cgggtcgatg cttgccgtgt cgaccccgat cgtgccgacg
     6601 cggcgttcgc ggatgagaaa ctccaccgcc tcgggcccat aagaggggaa gtgcagattg
     6661 gaggcatcgc caggcttgtt gtcgccgaga taggcgaggc gatccggcca gcgcgagccc
     6721 cagcccgtgc gcaaaagcac gatcgcgccc gacgcgatct tgccgtgctc ggcttcgaat
     6781 ttcgttatgt cctcgacggt caaggtgtag tcgcggttct ccttggcctt ttcgctgacg
     6841 tcgatgacga aggcggggcc gatgaagcgc tccacgggga tgtccgatcc gctccacacg
     6901 ccctctgcga agtggacagg cgcgtcgaga tgggttcctc catgctcggg cgcgcagaac
     6961 gcgtaggccg aatagtagaa gccgccggcg gtcaggccct tatgcagcgg cttaagctcg
     7021 aagccggacg gtgacgtcgg ccagtaaacg gtatccttgt cgaaggggtg ggtgagatcg
     7081 atgacgcggg aggccgaaag gtcgatctcc tggcttgagg ccggcgacgg ggagagcgcg
     7141 caaagggagc caagccatcc aaggaccagg acggagagaa gtgtgtggcg catcgcgttc
     7201 ctccttgggg ttcaagtgaa cgtcttcttc gtgccgtggc ttatggcgcc gcggcgagtt
     7261 tttcggccat ggcccagatc gccttgatct ggggcgcatc cttgccgaga tagtcgagcc
     7321 cggtatagcc ccaccaatcc caacagccat gccggttgac cgtgctcgcc ttcacctgtg
     7381 ggaacaggac gatgagccgg ttctggtcgg cgatctcggc gaagcccgag tccttgatga
     7441 aggtatcgcc gatttcctcg cgcgactggt cgcagccatg gagcgcgatg tggatccggc
     7501 agccgggccg atcggcgcag gccttaggga cataaaccac cccttcatct gcgaacccgt
     7561 cgccgggctc ggaaaaatcg ccttggtcga acacgatgaa gcggccggaa ggctccggcg
     7621 agggggccga tagcgggcca tagatccagg caaggatcgc ttgcgcttgg ttgtagttgc
     7681 agtcgctgac gaatggcgcc tcagaaatgc cgcaagccgc gccgccctgc tcggtcagga
     7741 aggcgtggcc gccctccttc tcgacgaggc tgatattgcc gggatcgacg ccggcttttt
     7801 tgtagaactc cgccgccgcc cgcaccaccg ggcgtgtgac cgtctcatct tcgttgcccg
     7861 agaagagata gacgttgtcg ggcttgaggc cggcgatggg gtcgatggcg cccgcgtcgg
     7921 ccagctcttt cgcccgttcg accgagaggg ccgggtccgg ctcgccccag tccgtcttca
     7981 tgcatttgtt gagcgcttgc gccgtgttct gggtcacggc gatcggccag aacgggaacg
     8041 cccggctcgc ggcactctcg gcgcaggcaa aggggccgcc cgcgacgatg ccggcgcccg
     8101 cgatgtcgct cgcgtgcgcc acctggatct gcacagccat gaaggcgccc gatgacaggc
     8161 ccgaga
//
LOCUS       DASUQP010000136        13286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_204744,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000136 DASUQP010000000
VERSION     DASUQP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204744"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..298)
                     /locus_tag="VFY74_06070"
     CDS             complement(128..298)
                     /locus_tag="VFY74_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931300.1"
                     /translation="MTGIEIVARVAELQKQYDDAHRPTRSLDAYRERHARRHPKRDYD
                     GEWHVSQYRPRG"
     gene            472..1206
                     /locus_tag="VFY74_06075"
     CDS             472..1206
                     /locus_tag="VFY74_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931301.1"
                     /translation="MTLFAASVVLGALLVASYGTRRIWLAGLSIALSAAVVAGVIFMA
                     TTETPAEGRAKLAWLAEKVPVVFDRQMLDTVTASLAYISTWAVQDSVSGGPPSVESSS
                     APPQPMQTASTTTSWFGADSAPKAKANSPVVWFLDDPNTPVVSPVEDGFAISGVNTSV
                     QDLSQVHATLKPDGSRREIALALKVEGRAAEGKAVIPAGARFSFGSETPEGAGAILTF
                     RYVYAGQQKAAILYLSADTIARFASR"
     gene            complement(1407..1955)
                     /locus_tag="VFY74_06080"
     CDS             complement(1407..1955)
                     /locus_tag="VFY74_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5931302.1"
                     /translation="MTREPIDETIPLRLGAPRRACALAIALLYGLATLSLLFAHAVAG
                     EATIIGERDGKDLVTVRVTEATTSKQALPNFLGISGKNAGAKSLSLLKVVIPPGGSAE
                     AHVHKGFESAIYLVQGRVETRYGEGLKKSVVNQAGDFIFVPPDVPHQPFNLSDTEPAI
                     AIVARSDPNEQEHTILYGGQTD"
     gene            complement(2000..2377)
                     /locus_tag="VFY74_06085"
     CDS             complement(2000..2377)
                     /locus_tag="VFY74_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEX5931303.1"
                     /translation="MGQEEQASPRIVHLSWGRIEVEGFRLFKDAKIFPGGAREWDWRE
                     TGTRHVPGIQPADIDELIAHGAKAVVLTRGIWKRLQVCSETLKALAEAGIETEVLQTE
                     AAVKRFNELRDILPTAGLFHSTC"
     gene            complement(2669..3397)
                     /locus_tag="VFY74_06090"
     CDS             complement(2669..3397)
                     /locus_tag="VFY74_06090"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX5931304.1"
                     /translation="MVKRANEAREMQPEIHITKNGPYLVMWPLSLTQVMIGTNDSGES
                     VRWESGRRFPPRPPMSLCRCGGSASKPYCDGTHARIGFDGTETASRKPYRELAKTIPG
                     PTASLMDAESLCAFARFCDPNGQVWTLVGETNDEAAKRDFVRQTCDCPSGRLVAVDNA
                     TGKPIEPHYEPSLALIEDPAEGCSGPLWVRGGVRLVGSDGFQYEPRNRVTLCRCGASK
                     NKPFCDGSHAAIKFRDGIAPEPNP"
     gene            3967..5508
                     /locus_tag="VFY74_06095"
     CDS             3967..5508
                     /locus_tag="VFY74_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5931305.1"
                     /translation="MADKENEGGVSYRSIDVGYFDDRELRRHAGVFYLWALGVAAVIS
                     GDFSGWNLGFAVGGWGGMFLGTVLITLMYLGLTYSIAEMSPALPHTGGAYSFARTAFG
                     PWGGYITGVAENVEYVLTPAVIVFFIGAYLTAIFGTPAAYQPVWWAVGYAFFVGLNVR
                     GVELSFTVTAVVTLLAIAVLVVFFVSAIPFIDFARYALNIGVDPSTGKGVELPGGNGP
                     FLPFGLYGVLAAMPFAVWLFLAIEQLPLAAEESVDPRRDMPKGIMLGMFTLIVLGFLV
                     LVINPAIPIERQVTDAGGATTTVHGAFALGTSAEPILDGFRAIFGGEAAGALALAAVI
                     GLVASFHAIIFASGRQIYSLSRAGYFPHVLSLTHGEHKTPNVAIVAGALVGFAVMLVV
                     WFGMGGERAGAFIGGVLLNMAVFGAMLSYLLQGLTFIQLRRHFPNIERPYRSPLGIPG
                     AAMAVIIALVTIGFQLTDPTYRMGVLGVAIWYALAIGYFLLYARKRLVLSPEEAFAVR
                     HRQDT"
     gene            5670..5858
                     /locus_tag="VFY74_06100"
     CDS             5670..5858
                     /locus_tag="VFY74_06100"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX5931306.1"
                     /translation="MGSFHSPKRYGSKTRIDLNQSYQVAYWKDRFGVTEEELSEAVHA
                     AGALARKVEKYLKAKVDR"
     gene            complement(5921..6058)
                     /locus_tag="VFY74_06105"
     CDS             complement(5921..6058)
                     /locus_tag="VFY74_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931307.1"
                     /translation="MFEWINSVSIVTMITAIVLLDQPFQGAVRVSPDAYELVYDRMMK
                     Q"
     gene            complement(6301..7086)
                     /locus_tag="VFY74_06110"
     CDS             complement(6301..7086)
                     /locus_tag="VFY74_06110"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX5931308.1"
                     /translation="MLDWVYSTHPAMVVGAIVLVFVAMCWLGIFALRPVVKHLVQQQP
                     GLNETVGDFLQYFGVIYGLLLGLLAVATYQNLSDVEKTVGNEAAALGALYRDVSAYPE
                     PARSEVQAMIRDYTRYVIDEAWPLQQKGIVPPGAVAKAARIFERLVAFEPQSKSQEAL
                     HQETLRQFNTFFHHRRERLHSVTFAIPPLLWYTVAVGAVVNMLLMWLFDLRIGIHLLL
                     GGTLSFFLATMISLILLMDQPFRGDVSVSSSAFELIYDQLMTN"
     gene            7674..8069
                     /locus_tag="VFY74_06115"
     CDS             7674..8069
                     /locus_tag="VFY74_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931309.1"
                     /translation="MRGDAVVRGFSGTVVPDPLPAGDPLDETFIDLDGASAPIFRLEP
                     GAPPSAQVISAPPVFQVKARDVGQVFAIGLDGNLVPTPDPPNIYLGATSAFGLQIVIP
                     DADGDGRPFICEEGIFPEIKSEKARKCEV"
     gene            8237..9592
                     /locus_tag="VFY74_06120"
     CDS             8237..9592
                     /locus_tag="VFY74_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5931310.1"
                     /translation="MIEKRNRNGGKDRDRDDEQDEDAGSPAPDSGPVARKEENAPSRA
                     APPMVAPPSRLGGAFARLILLLLVIAAAALLYRYWGFWQAEPAAQAAPPAPPVTVAKP
                     LVNEMEEWNDFTGQFEAQEAVEVRARVSGYLESVNFTDGQLVKKGDLLLVIEPRPFEL
                     ALETSKAQLAQAEAQLQLAQAQLDRTAQLRKNDYATKETYDERVSQVAIAAASRDAAI
                     AAVNQAQLNLDYTRVTAPVSGRTGRHEVSVGNLVTGGTAGSTTLLTTIVSLDPIWLVF
                     NVSEGDGMTYKRLVQKGEIKSARDNVIDVQGQLMDERDWPLKGMIDFVDNQYNRTSGT
                     IRVRAEFPNADLFITPGQFGRVRVPMSKTRPTMLVPDSAVVTDQSVKVLFTVTADDTV
                     VPKMVELGPVTDDGLRIIRSGITPDDRVIINGLLRAQPGGKVKPEEGTISAPGAPPQP
                     S"
     gene            9589..12774
                     /locus_tag="VFY74_06125"
     CDS             9589..12774
                     /locus_tag="VFY74_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX5931311.1"
                     /translation="MNFSHFFIDRPIFAAVISIIITLIGGVAYFNLPVAQYPDIAPPS
                     ISVNASYPGASAEIVAKTVATPLEQEVNGVDNMLYMTSQSTSDGSMSLTVTFKLGTNL
                     DTAQVLVQNRVTTALPRVPEEVRAIGVTVTKQSPDLMLVIHLSSPDGSRDLLYISNYA
                     TLHVKDVLARIEGVGNVTIFGARDYSMRIWLDPDRLAVRNLTAGDVVTALRGQNVQVA
                     AGVINQPPVPKPGAFQLNVETQGRLTTTEAFGNIIVKSESDGRVVRIRDIGRVELGAA
                     DYNRSGYLDERVAIPLGIFQRPGSNALATAAQVQKAMAQLAQSFPYGLRYDIVYNPTV
                     FISQSVEAVITTMVEAVVLVVVVIILFLQTWRASIIPIVAIPVSLIGTFAMLSALGFS
                     LNNLSLFGLVLAIGIVVDDAIVVVENVERNIREGLSPRDAAYKTMEEVGGALVAIALV
                     LSAVFIPAAFITGITGQFFRQFAVTIATATAISLLVSLTLSPALCALLFKPHEAEPAK
                     RSIFTRPIRAFFKGFNRAFEWISSRYGALTGRLVRISLLMLLVYGGLIGLTVYQFIKA
                     PTGFIPPQDQGYLINIIQLPPGASLARTDEVARKVTKLVLDTPGIAHAVPIVGLDGAT
                     FTTAPNAAVVFTPLDPFEERAKKGQSAQSLILALNTKYAAVQDAFIVSVSPPPVRGIG
                     TTGGFKMQVQDVGGGEPAQLEGVAQDVVAGAAQEPGLANVFTTFNTRTPKVYADIDRV
                     RAEMLGVNPDDVFQTLEVYLGSQYVNDFNFLGRTYRVTAQADGDFRQDLHAIGQLKMR
                     NAEGQMVPLSSVASFRDITGPYRIERFNLFPSAAVQGGTKPGYSTGYGLAAMERIARE
                     VLPAGYAYSWTEIAFQEKLAGNTAIYVFLASVVFVFLLLAAQYESWTLPIAVILIVPM
                     CLLAAVSGLLARGMDVNILAQIGFVVLIGLAAKNAILIVEFAKQNEEHGEDRFQAATE
                     AARVRLRPILMTSFAFILGVLPLVIATGAGAEMRQSLGTAVFYGMIGVTFFGLIFTPV
                     FYVVMRKFARNSRFQGTTTTRKRALPLSMRW"
     gene            complement(12720..>13286)
                     /locus_tag="VFY74_06130"
     CDS             complement(12720..>13286)
                     /locus_tag="VFY74_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5931312.1"
                     /translation="AGITTFNALRNAGARAGDVVAIQGIGGLGHLGVQFANRMGFHTV
                     AIARGKDKEKLATRELGAHVYIDSETQDPAKEMVALGGAKVILATATSGKSMTPLIGG
                     LGVDGTLIVVGASPEPIEVNPYYVIGDRRGVRGWPSGTSVDSEDTMRFSALTGVAAMI
                     ETYPLTRAAEAYQRMLSGKARFRVVVVP"
BASE COUNT         2365 a         4258 c         4131 g         2532 t
ORIGIN      
        1 gctcgcccac ctctcccacg acctggctgc gcgaagccgg agcttcgttc cggcgaaagt
       61 aggggagggg aaaagcaatg cgccgttgtc cgctctggat tccggattgc cccgaaggat
      121 gaaggtttca gccccgcggc cggtattggg agacgtgcca ctcgccgtcg tagtcgcgct
      181 tggggtggcg cctggcgtgg cgctcgcgat acgcatcgag ggaccgcgtg ggcctgtgcg
      241 catcgtcgta ttgcttctga agctcggcca ctctggcgac gatttcgatg ccggtcattg
      301 ccgaatcctc cgctcaaatc gcccattctt gagggttcga cgccgcggcg gcgcaaaagg
      361 ttcaagccct tgaggccggc tgaaagtagg caaattttca ttcatttccc ccaattggcc
      421 gtgctgccgc cacggttttg tgccgtgatg ggcggaccta cgggggaaga attgaccctt
      481 ttcgctgcca gcgtcgttct cggagcgctg ctggtcgcgt cctatggaac gcgacgcatc
      541 tggcttgcgg gcctttcgat cgcgctatcg gccgctgtcg tggccggggt catcttcatg
      601 gcaaccaccg aaactcccgc cgagggccgc gcgaagctcg cctggctggc ggagaaggtt
      661 ccggtcgtct tcgaccggca aatgctcgat acggtcactg cgtccctcgc atatatctcg
      721 acgtgggcgg tgcaagacag cgtcagcggc ggtccgccaa gcgtggaatc gtcttcggcc
      781 ccccctcaac ccatgcagac ggcgtcgacg acgacgagct ggttcggcgc tgacagcgcg
      841 ccgaaggcca aggcgaattc gccggtcgtc tggtttcttg acgatccgaa cacgcctgtg
      901 gtctcgccgg tcgaagatgg gttcgcgatc agcggggtga acacgtccgt tcaagacttg
      961 agccaagtgc acgcgacgct caagcctgac ggaagccggc gcgagatcgc tcttgctctc
     1021 aaggtcgagg gacgcgcggc cgaaggcaag gccgtcattc cggcgggtgc gcgcttcagc
     1081 ttcggctccg aaacgcccga gggcgccggc gccatcctga cgttccgcta cgtctatgcc
     1141 ggacagcaaa aagcggcgat cctctatctc agcgcagaca cgatcgcgcg gttcgcaagc
     1201 cgctgatcat tcgccctcat aacttggctt gagtcagata tctccttggt catggccggc
     1261 ttgtcccggt catccacgtt tgggtttgcc gctccaaaaa tcgtggatgg ccggcacttc
     1321 ggccggccat gacgaagatc ggagccatcg gctacggctt gcagccccgg taatgccgcg
     1381 acgcgggttc cgggaatgac gggcgttcag tccgtctggc cgccatagag aatcgtgtgt
     1441 tcctgctcgt tcggatccga gcgcgccacg atggcgatgg cgggttcggt gtcgctcaga
     1501 ttgaacggct gatgcggcac gtcaggcgga acgaaaatga aatcgccggc ttgattgacg
     1561 acgcttttct tcaagccctc gccgtaccgg gtctcgacgc ggccttgcac gagatagatc
     1621 gccgactcaa agcccttgtg cacatgggcc tcggccgagc cgcccggcgg aatgacgacc
     1681 ttcaacaggg agaggctctt tgcgccggcg ttcttgcccg agatcccgag gaagttcgga
     1741 agcgcctgct tcgatgtcgt ggcttcggtc acgcgcaccg tgacgagatc tttgccgtcg
     1801 cgttcgccga tgatggtcgc ctcgcccgcc acggcatgcg cgaacaagag agagagcgtg
     1861 gcaagaccat agaggagggc gatcgccaag gcgcatgcgc ggcgcggcgc accgaggcgt
     1921 agggggatcg tctcgtcaat cggttcgcgg gtcatgcgct ttgctgcctt cttgccatgg
     1981 gtggcgcccg cgcgtttcat caacaggtcg agtgaaacag gccggcggta ggaagaatgt
     2041 cgcggagttc gttgaagcgc ttcaccgcag cctcggtctg caagacttcg gtctcgatcc
     2101 cggcctcagc cagggccttc aaagtttcgg aacagacctg aagccgcttc caaattccgc
     2161 gcgtaagcac caccgccttg gcgccgtgcg cgatcaactc gtcaatgtcg gctggctgaa
     2221 tgccgggcac atggcgtgtt ccggtttcgc gccagtccca ttcgcgcgct ccgccgggaa
     2281 agattttcgc gtccttgaag agccgaaagc cttcgacctc gatgcggccc catgacaggt
     2341 gcacgatccg cggggaagcc tgctcctctt ggcccatgtt gtgtcccgag aagggagcgt
     2401 agcccacatc aaaagcaaaa aggggaaagc caagccttcc cggccaggct tcccccgtct
     2461 cttgcgcttt cccccgaaat gctacgcgcg aaatcgcctt aacaagaatg catgagtcgg
     2521 gcgattatcg ggcaagtttg cggcacgcga agaattgaga tggaccgttg cgcaaatgtc
     2581 acgcctttag agaaccggcc gtttgcttca attccgccgc gacaggagct ctgcccaagc
     2641 accgcgagcc cgcgacttca ccccgcgttc acgggtttgg ctccggcgca atcccgtccc
     2701 tgaacttgat cgccgcatgc gatccatcgc agaacggctt gttcttcgaa gcgccgcagc
     2761 ggcacagagt gacgcgattg cgaggctcgt attggaagcc gtccgagccc accaggcgaa
     2821 cgcctccccg cacccagagc ggaccggaac agccctcggc cgggtcctcg atgagagcaa
     2881 gcgatggctc gtaatgcggc tcgatcggct tgcccgtggc attgtccacg gcaaccagac
     2941 ggcccgaggg acagtcgcag gtctgacgca cgaaatcgcg ctttgccgct tcgtcgttcg
     3001 tctcgccgac gagggtccag acctgaccgt tggggtcgca gaagcgcgca aaggcgcaga
     3061 ggctctcggc atccatcagc gaggcggtcg gacccggtat cgtcttggca agctcgcgat
     3121 agggcttgcg gctcgcggtc tcggtgccat cgaagccgat tctggcgtgc gtgccgtcgc
     3181 aataaggctt gctcgcgctt ccgccgcacc ggcaaagcga cattggcggc cgcggcggaa
     3241 atctacggcc cgattcccag cgcacggact cgcccgagtc attcgtgccg atcatgacct
     3301 gggtcaggga caaaggccac atgacgagat agggaccgtt tttcgtgatg tgaatttccg
     3361 gttgcatctc gcgtgcttcg ttggcgcgct tcaccacgct ggcattcggt caaaaggtga
     3421 acccagcgtc attccggcgc aagctggaat taaggggctt gctcaccgtt gcccgccgcc
     3481 gatgagcgcc aagcaccgtc tccaacggcc ggaggagctt ggatcggctc gcgttatgga
     3541 ggagaggcgt cgcgccgcat gtctgccggc cgtgcggtgt cgcaatcctg cctacgatcg
     3601 cagcggaccg ggagacaaag catgtgcaag tgacggtggt acacgcgctc gcggccgaaa
     3661 tccttgagtg ggccgcaggc gcaagaatag tggcaaccat cgacgtcatg tctgaggcgg
     3721 aattgggcgc tgtccttgaa gcccgcgcct ctcgggtcgg catttgccgc aggcggagcc
     3781 aagacaaggt gaagagcgaa agcggcagcg aggagaaggg caggcttgct cggtctcatt
     3841 tcgcacggtc ggaagcgatt gaggctcgcc gattgtcgcc cacaagcgct ttccacgcta
     3901 tggtctagga cctccagggc tcggcccggc cataaatctg ccggggtgac gggcaagaga
     3961 agcactatgg ccgacaagga aaatgagggc ggcgtttcct atcgcagcat cgacgtaggc
     4021 tatttcgacg accgggagct caggcgccat gccggcgtct tttatttatg ggcgctcggc
     4081 gtggccgccg tcatctcggg agacttctcc ggctggaacc tgggcttcgc cgtcggcggc
     4141 tggggcggca tgttcctcgg caccgtcctc atcaccctca tgtatctcgg cctcacctat
     4201 tccatcgcgg aaatgagccc ggcactgccg cataccggcg gcgcctactc ctttgccagg
     4261 acggcgttcg gaccatgggg cggctatatc accggcgtcg ccgagaatgt cgaatacgtg
     4321 ttgacgccgg ctgtaatcgt cttctttatc ggcgcctacc tgaccgcgat cttcggcacg
     4381 cctgccgcct atcagcccgt ctggtgggcc gtcggctacg ccttctttgt cgggctgaac
     4441 gtccgcggag tcgagctgtc gttcaccgtc accgccgtcg tgaccctgct tgcgattgcc
     4501 gtcctcgtcg tcttttttgt cagcgcgatt cccttcatcg attttgcgcg gtacgcgctc
     4561 aacatcggcg tcgatccgtc aacggggaaa ggcgtcgagc tgcccggggg aaatggtcca
     4621 ttcctgccct tcggccttta cggggtcctc gccgcgatgc cgtttgccgt gtggctgttc
     4681 cttgccatcg agcagcttcc gctcgccgcg gaggaatcgg tcgatccgcg ccgcgacatg
     4741 ccaaagggca tcatgctcgg catgttcacg ctcatcgtgt tgggatttct ggtgctcgtc
     4801 atcaacccgg cgattcccat cgagcggcaa gtcaccgacg ccggcggcgc gacgacgacg
     4861 gtccatggcg cctttgcgct cggaacctcg gccgagccga ttctcgacgg cttccgcgcc
     4921 attttcggag gggaggcggc gggcgcgctc gcgctcgccg cggtgatcgg cctcgttgcc
     4981 agcttccatg ccatcatctt cgcctctggc cgccagatct attcgctgtc acgcgcaggt
     5041 tatttcccgc acgtcctctc gctcacccat ggcgagcaca agacgccgaa cgtcgccatc
     5101 gtcgcagggg cgttggtcgg ttttgccgtg atgctggtcg tctggttcgg catgggcggc
     5161 gaacgggcag gagcgtttat cggcggcgtt ctgctcaaca tggcggtgtt cggcgcgatg
     5221 ttgtcctatc ttctccaggg cctgaccttc atccagctgc ggcggcactt tccgaatatc
     5281 gagcgtccct acaggagccc cctgggcatc cccggcgccg cgatggccgt gatcattgcg
     5341 ctcgtgacga tcggattcca actcaccgat cccacctatc ggatgggagt cctcggcgtt
     5401 gcgatctggt acgcgctggc gatcggctac tttcttctct acgcgcgcaa gcgtttggtg
     5461 ttgtcgccgg aagaagcgtt cgccgtcagg catcgtcagg acacgtaggc gaaggtctac
     5521 caccctcggg ggcacaaaag gtcttggcgg ccgaaggcgt gttagggcat gctgaggcaa
     5581 acccgcctga accccgacgg agcgcgaggg agcgccggct gggcttgggc cacggacatg
     5641 acactgcgtg cgccacagga gaagccgaga tgggcagttt ccactcaccg aagcgctacg
     5701 gcagcaagac ccgcatcgac ctgaaccagt cctatcaggt ggcctattgg aaggatcggt
     5761 tcggcgtgac ggaagaagaa ctcagcgaag cggtgcacgc cgcgggcgcg ttggcaagaa
     5821 aggtcgaaaa atacctcaaa gccaaggtcg atcgctagct tcgccggccg cgccggcgcg
     5881 gaccgcctga cggcaaacct gcttcggcag aacggcggac ttactgcttc atcattcgat
     5941 cgtagacgag ttcataggcg tccggcgaca cgcggaccgc gccttggaac ggctggtcca
     6001 gcaacacgat cgccgtgatc attgtgacga tggagacgct gttgatccat tcgaacatgg
     6061 ctggctccga ttgcgtgcct cgccatgcgt agcgccacgg ggtgaacagc ttcctaagag
     6121 acgtccaccc gctaccagcg gcttgccgcc cgcgctgatt gcgtcccgtt gtggcggacg
     6181 aagaccccat cggacgcgtt gggatggcga gagaggccgg cccgggccct tcttcgatgt
     6241 ggaagggacg gattgcagcc ggcattgacc tcgcaacccg gttcgaattc gagggcggtc
     6301 tcagttcgtc atcagctggt cgtagatcaa ctcaaaggcg gatgacgata cgctcacatc
     6361 tccgcggaac ggctggtcca tcaagaggat cagcgaaatc atcgtcgcaa ggaagaagga
     6421 gagggtgcct ccgagcagca agtggattcc gatgcggaga tcgaacaacc acatgagcag
     6481 catgttcacc accgctccga ccgcaaccgt gtaccagagg aggggtggaa tggcgaatgt
     6541 cacgctgtga aggcgctcgc ggcgatgatg aaagaatgtg ttgaattgcc gcaaagtctc
     6601 ttggtgcagc gcttcctgtg atttcgactg tggctcgaag gcgacgagcc gctcgaatat
     6661 gcgggcggct tttgcgaccg cccctggggg gacgatgccc ttctgctgaa ggggccaagc
     6721 ttcatcgata acataacggg tgtagtcgcg gatcatggcc tggacttccg agcgggccgg
     6781 ctcggggtag gcgctgacat cgcggtacag agcgccaagc gccgccgcct cattgccgac
     6841 cgtcttctct acatcagaaa gattctgata ggtcgctacc gcgaggagac ccagaaggag
     6901 gccatagatc acgccgaaat attgcaggaa atcgccgacc gtctcgttga gacccggctg
     6961 ctgttgaacc aagtgtttga cgacaggtct gagcgcgaaa atgcccaacc agcacatcgc
     7021 aacaaaaacg agcacgatgg ccccgacgac catcgcggga tgggtgctgt aaacccaatc
     7081 gagcatgacg tccccccttc gagtatcggc gcctctccct cgcacaacgc taatcggccg
     7141 gcagcatcgg cctgaccgac aacgagacaa taaagagtgc tggcgcgaag agataagcgg
     7201 ccaacttggc gcgatggcgc gatcgtgccg ccgctggatg cctcccccct gccactcttt
     7261 tgctgaactg gcgcgccgcc aaggctgccg ttcccccgca gccaagctag cagcggcgcc
     7321 tggaagcgac aagcgaacgc tctgggttcg gctaccgtcg gaaattctca gtccaggcct
     7381 cgagattcat cgaaatccgt ttgaaccctt caccgggcgg aggcgaccaa cacggcagaa
     7441 cggcctggtg tcgtctcgta gtgatggaga gctgcgatga atgctggtaa actcttccgt
     7501 atcggctcgg cagcgcgcga cccgagcgtc ggcgctatgg ccaggaatgc gacgatgacg
     7561 cgtcccgtcc tgtccgcgtt cccgtgttgg cgcctcctgc tcggggcgac gctctccctc
     7621 ttcgtgacct tggccgccct tccgctcaat gcccaggacg ccgtgatttt cgggtgcggg
     7681 gtgacgcggt ggtcaggggc ttctccggga cggtggtccc cgatccgctg ccggctggag
     7741 accctctcga tgagaccttc atcgatctcg atggcgcgtc ggcgccaatt ttcaggctcg
     7801 aacctggcgc accgccttcg gcccaggtca tttccgcgcc gcccgtcttt caggtcaagg
     7861 cgcgcgacgt cggccaggtg ttcgcaatcg gattagacgg aaatcttgtc ccgactcccg
     7921 atccgcccaa catctatctt ggcgcgacct ctgccttcgg tcttcagatc gtcattcccg
     7981 atgccgacgg cgacggccgc cccttcattt gcgaggaggg catttttccg gagataaagt
     8041 ccgaaaaagc acggaaatgt gaggtttgag ccaaggcgcc aggcttgccc gcaagggcat
     8101 tcggaggcga gactggggcc ggatgattgg cgattccttg aggctggcgg ccaatcaagc
     8161 tcgccttggc cgccgccttt ggatagcgtc cccttatgcg aggcgtccca agccttcatc
     8221 agcatgagat gcggttatga ttgagaagcg aaaccggaac ggcggcaagg accgcgaccg
     8281 ggacgacgaa caagacgagg atgccggttc gcccgctccc gatagcgggc ctgtcgcgcg
     8341 gaaggaagag aacgcgccat cgcgcgccgc gccgcctatg gtagccccgc cgtcgcgcct
     8401 gggcggcgcc ttcgcccgcc tcattcttct cctgctcgtc atagccgccg cggcgctgct
     8461 ctatcgctat tggggcttct ggcaagccga gccggcagcc caggccgcgc cgccggcgcc
     8521 gcccgtcacc gtcgccaagc cgctggtcaa cgagatggag gagtggaacg atttcacagg
     8581 acagttcgag gcgcaggaag cggtcgaagt gcgcgcgcgg gtcagcggtt atctggagtc
     8641 cgtaaatttc accgacggcc agcttgtcaa gaagggcgac cttctcttgg tcatcgagcc
     8701 gcgcccattc gaactcgcgc tggagaccag caaggcgcag ctcgcccagg ccgaggccca
     8761 gctgcagctg gcgcaagccc agctcgatcg caccgcccag ctgcgcaaga acgactacgc
     8821 caccaaggaa acctatgacg agcgggtgtc gcaggtcgcc atcgccgcgg catcgcgcga
     8881 tgcggcgatt gccgcggtca accaggcgca gctcaatctc gattatacca gggtcaccgc
     8941 tccggtctcg ggccgcacgg gccgtcatga agtcagcgtc ggcaatctgg tcacgggcgg
     9001 aaccgccggc agcaccacgc tgctcaccac catcgtttcg ctcgatccga tctggctcgt
     9061 gttcaatgtc agcgaaggcg acggcatgac ctataagcgc ctggtgcaga agggggagat
     9121 caagtccgcc cgggacaatg tcatcgacgt gcaaggccag ttgatggacg aacgcgattg
     9181 gccgctcaag ggcatgatcg acttcgtcga caaccaatat aaccgcacgt cgggaacgat
     9241 ccgcgtgcgg gccgaattcc ccaatgccga cctgttcatc acccccggtc agtttggccg
     9301 tgtgcgcgtg ccgatgtcga agacgcgccc gaccatgctg gtgccggatt ccgcggtggt
     9361 caccgaccag tcggtcaagg tcctgttcac ggtcaccgcc gacgacaccg tcgtgccgaa
     9421 gatggtcgag ctcgggccgg tcaccgatga cggcttgcga atcatccgca gcgggatcac
     9481 cccggacgat agggtcatta tcaatgggct cttgcgggcg cagcccggcg gaaaggtgaa
     9541 gcccgaggaa ggcacgatca gcgcgcctgg cgcgccgccg caaccctcat gaatttctcg
     9601 catttcttca tcgaccggcc aattttcgcg gcggtcatct ccattatcat cacgctgatc
     9661 ggcggcgttg cctatttcaa cctgccggtg gcgcaatatc ccgacatcgc gccaccctcg
     9721 atctcggtga atgcgagcta tcccggcgcc tcggccgaga tcgtggccaa gacggtcgca
     9781 acgccgctcg agcaggaggt caacggcgtc gacaacatgc tctacatgac gtcgcagtcg
     9841 acgagcgacg gcagcatgtc gctcacggtc accttcaagc tcgggaccaa tctcgatacg
     9901 gcgcaggtgc tggtgcagaa ccgcgtgacc acggcgctgc cccgcgtccc cgaggaggtg
     9961 cgcgcaatcg gcgtgaccgt caccaagcaa tcgcccgacc tgatgttggt gatccaccta
    10021 agctcacccg acggctcgcg cgacctgctc tacatctcga actacgccac cctgcacgtg
    10081 aaggacgtgc tcgcgcgtat cgagggggtt ggcaacgtca cgatcttcgg cgcccgtgac
    10141 tactcgatgc gcatctggct cgatcccgac aggctggcag tgcgcaatct caccgcaggc
    10201 gacgtggtta ctgcgctgag gggacagaac gtgcaggttg cggccggggt catcaatcag
    10261 ccgccggtgc cgaagccggg cgccttccag ctcaacgtcg agactcaagg acggctgacc
    10321 accaccgagg ccttcggcaa tatcatcgtc aagtcggagt ccgacgggcg ggtggttcgc
    10381 attcgcgata tcggccgcgt cgagctcggc gccgccgact acaaccgcag cggctatctc
    10441 gacgagcggg tcgcgattcc cttgggcatt ttccagcggc cgggctccaa cgcgctggcc
    10501 accgcggccc aggtccagaa ggccatggct cagctcgcac agtccttccc gtacggactg
    10561 cgctacgaca tcgtctacaa tccgaccgtc ttcatctcgc agtcggtcga agcggtgatt
    10621 accaccatgg tcgaggccgt ggtcctcgtg gtggtggtga tcattctgtt cttgcagacc
    10681 tggcgcgcgt ccatcattcc catcgtggcc attccggtct cgctgatagg gaccttcgcc
    10741 atgctgtcgg cgctcggctt ctcgctcaac aatctttcac tgttcggtct cgtgctcgcg
    10801 atcggcatcg tggtcgatga tgccatcgtc gtggtggaga atgtcgagcg caacattcgc
    10861 gaaggcttga gcccgcgcga cgccgcctac aagaccatgg aagaagtcgg cggcgcgctc
    10921 gtggcgatcg cgctcgtatt gtcggccgtt tttattcccg cggccttcat caccggcatc
    10981 accggccagt tcttccgcca gttcgcggtg acgatcgcca cggcgacggc aatctcgctg
    11041 ctcgtctcgc taacgctcag tccggcgctc tgcgcgcttc tgttcaagcc gcacgaagcg
    11101 gagccggcga agcgatcgat cttcacccgg ccgatcagag ccttcttcaa agggttcaac
    11161 cgcgcgttcg aatggatctc ttcccgttat ggcgcactca ccgggcggtt ggtccggatc
    11221 tcgctcctca tgctcctcgt ctatggcgga ctgatcggac ttaccgtcta tcagttcatc
    11281 aaggcgccga ccggtttcat cccgccgcag gaccagggct acctgatcaa tatcatccag
    11341 cttccaccgg gcgcctcgct cgcgcgcacc gacgaggtgg cgcgcaaggt gacgaagctc
    11401 gtgctcgaca ccccgggcat tgcgcacgcc gtgcccatcg tcggtctcga cggcgccact
    11461 ttcaccaccg cgcccaacgc cgcggtggta ttcacgccgc tggacccgtt cgaggagcgc
    11521 gcaaagaaag gccagagcgc gcagtccctc atcctcgccc tgaacacgaa atacgccgcg
    11581 gtccaggacg ccttcattgt tagcgtctcg ccgccccctg tgcgcggaat cggcactacc
    11641 ggcgggttca agatgcaggt gcaggacgtc ggcgggggcg agcctgctca gctcgaaggc
    11701 gtggcgcagg atgtcgtcgc cggcgccgcg caagagccgg gcctcgccaa cgtgttcacg
    11761 accttcaaca cgcgcacgcc gaaggtctac gccgatatcg accgcgtccg ggccgaaatg
    11821 ctcggcgtca accccgacga tgtgtttcaa actctggagg tctatctcgg atcacaatat
    11881 gtcaacgatt tcaatttctt gggacgcacg taccgggtca cggcccaggc cgacggcgac
    11941 tttcgtcagg atctccacgc catcggccag ttgaagatgc ggaatgccga aggccagatg
    12001 gtgcccttgt catcagtcgc ctctttccgc gacatcactg ggccttaccg catcgaacgt
    12061 ttcaacctgt tcccgtcggc ggcggttcag ggcggcacca agccgggcta ctccacaggc
    12121 tatgggctcg ccgccatgga gcggatcgcg cgagaggttc tcccagcggg ctacgcctat
    12181 tcgtggaccg agattgcctt ccaggagaag ctcgcgggca atacggcgat ctatgtcttc
    12241 ctcgcttccg tcgtgttcgt gtttctgctg cttgcggcac aatacgaaag ctggacgctg
    12301 ccgatcgcgg tcatcctcat tgtgccgatg tgtctgctcg cggcggtgag cgggctgctc
    12361 gcccgcggta tggacgtaaa tattctcgcc cagatcggtt tcgtcgtgct gatcggactt
    12421 gccgccaaga acgcgattct gatcgtcgaa ttcgcaaagc agaacgagga gcatggcgag
    12481 gaccgcttcc aggccgcgac cgaagcggca cgcgtgcggc ttcgcccgat cctcatgacc
    12541 tccttcgcct ttattctggg cgtgctgcct ctggtgatcg ccaccggcgc aggcgcggag
    12601 atgcgtcagt cgctcggcac cgccgtgttc tacggcatga tcggcgtcac ctttttcggc
    12661 ctgatcttta cgcccgtatt ctatgtggtg atgcggaaat tcgcgcggaa tagccggttt
    12721 caaggaacga cgacgacgcg gaagcgggcc ttgccgctca gcatgcgctg gtaggcttcc
    12781 gccgcgcgcg tcagcggata ggtctcgatc atcgccgcga cgccggtcag cgcgctgaat
    12841 cgcatcgtgt cctccgaatc gaccgaggta cccgacggcc agccgcgcac gccgcgccga
    12901 tcgccgatca cgtaataagg attgacctcg atcggctcgg gcgatgcgcc gaccacgatc
    12961 agcgtgccgt cgacgccgag cccgccgatc agaggcgtca tcgacttgcc gctcgttgcc
    13021 gtggcgagga tcactttcgc cccgccgagc gccaccatct ccttggcggg atcttgcgtc
    13081 tcgctgtcga tataaacatg ggcgcccagc tcgcgcgtcg ccagcttctc cttgtccttg
    13141 ccgcgcgcga tcgccacggt atggaaaccc atccggttcg caaattgcac gccgagatgg
    13201 ccgagaccgc cgataccctg gatcgccacc acatcgccgg cgcgcgcgcc cgcgttgcgg
    13261 agcgcgttga aggtggtgat gccggc
//
LOCUS       DASUQP010000137         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1158709, whole genome shotgun sequence.
ACCESSION   DASUQP010000137 DASUQP010000000
VERSION     DASUQP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158709"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFY74_06135"
     CDS             complement(<1..657)
                     /locus_tag="VFY74_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5931313.1"
                     /translation="MRVVQVANPNAPLELVERPVPEPGPREARIKIEACGICHSDSYV
                     VTGTYPGVRYPAVPGHEIAGRIDAVGPGVTTWRVGQRVGVGWFGGVCWTCPRCRRGDF
                     ITCRNLRVPGIHYDGGYADYMIAPIEALASIPDDLDAAAAAPLLCAGITTFNALRNAG
                     ARAGDLVAIQGIGGLGHLGVQFANRMGFHTVAIGRGKDKEKLARDLGAHVYIDSETQD
                     P"
     gene            complement(685..1329)
                     /locus_tag="VFY74_06140"
     CDS             complement(685..1329)
                     /locus_tag="VFY74_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX5931314.1"
                     /translation="MIETFLTAFTTFFAVIGPIDTAVLLFSLIPNMTGAERRAISVKA
                     VFIATIIILLFALVGQPVLTQFGVSLAALQTAGGIILFMIALEMTLARRPGPAAALSP
                     RESEEVEDKAERHAEIAVFPLATPLLAGPGAMTSAIVLAAGTKGDISLIGAVIAAIIA
                     VMAVTLVLLLLAQEIHQVIGVTPRKVIVRVFGVLLAALAVQSIFNGLSQADIFG"
     gene            complement(1538..>2173)
                     /locus_tag="VFY74_06145"
     CDS             complement(1538..>2173)
                     /locus_tag="VFY74_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5931315.1"
                     /translation="QLVVSGNIGPRGDGYDPGAVMSPWEAEAFHAWQIGVFADAGADL
                     VSAFTLTNVNEAIGVARAAKAAGMPCVISFTLETDGKLPTGEGLAAAIASVDRETNQG
                     PAYYMINCAHPDHFSDALDQDAAWTKRLRGLRANASRKSHAELDNATELDAGDPHELG
                     HLYAELLRRFPHINVLGGCCGTDHRHMACISAACRDSAHRVDARPAPQAAE"
BASE COUNT          406 a          677 c          748 g          342 t
ORIGIN      
        1 aggatcttgc gtctcgctgt cgatataaac atgggcaccg agatcgcgcg cgagcttctc
       61 cttgtccttc ccgcggccga tcgcaacggt atggaatccc atccggttcg caaactgcac
      121 gccgagatgg ccgagcccgc cgatgccctg gatcgccacg agatcgccgg cgcgcgcacc
      181 cgcattgcgg agcgcgttga aggtggtgat gccggcgcac agaagcggcg ccgcagcggc
      241 ggcatcgagg tcgtcgggaa tcgacgcgag agcctcgatc ggcgcgatca tgtaatcggc
      301 atagccgccg tcatagtgaa tgccggggac tctgaggttg cggcaggtga tgaagtcgcc
      361 gcgccggcag cgcggacaag tccagcagac gccgccaaac cagccgacgc cgacgcgctg
      421 gcccacgcgc caggtggtga cgccgggtcc gaccgcatcg atgcggccgg cgatctcgtg
      481 gcccggaacg gccggatatc tgacaccggg gtaagtgcct gtcaccacat aggaatcgct
      541 gtggcagatg ccgcaagcct cgatcttgat gcgcgcctcg cgcggacccg gttcgggcac
      601 cggccgttcc accagctcga gcggcgcatt gggatttgcg acctggacca cgcgcatgac
      661 agactcctcc tacctcaact cggctcagcc aaagatatcg gcctgagaga ggccgttgaa
      721 gatcgattgc acggcgagcg cggcaagcag cacgccgaac acccgcacga tcaccttgcg
      781 cggcgtcacg ccgatcacct gatgaatctc ttgcgcaagc aggagcagca cgagcgtgac
      841 ggccatcacc gcgatgatcg cggcgatgac ggcgccaatg aggctgatgt cccccttggt
      901 accggcggcg agcacgatgg cggaagtcat ggcgcctggg ccggcgagca gcggcgtggc
      961 gagaggaaag acggcgatct cggcatgtcg ctcggccttg tcctccacct cctcgctctc
     1021 tcggggcgag agggcggcgg cgggaccggg acgccgcgcg agtgtcatct cgagagcgat
     1081 catgaacagg atgatgccgc ccgcggtctg aagggccgcg agcgagacgc cgaactgggt
     1141 caagaccggc tgcccgacaa gcgcgaagag aaggatgatg atggtggcga tgaacaccgc
     1201 cttgaccgag atggcgcgtc gctcggcccc ggtcatattg gggatgagcg aaaaaagcag
     1261 cacggccgtg tcgatggggc cgatgacggc aaaaaacgtg gtgaaggcgg tgagaaacgt
     1321 ctcgatcatg gagcggtgag ggggcttggg gcgcgctcgc ttctaccatt tttcgcaaag
     1381 gaggatcggc caccatgtga ccccttgcga cttcccggag acaggcgcga cctgggtcgc
     1441 gctcgggcgc gaagcaggat tcgcgaacgc gcgcgagctg ttatgcgacc cgacaggctt
     1501 ctaccgcatc catcgctaag agcgctgaag ggggacgtta ctcggcggct tgcggcgcgg
     1561 gccgcgcgtc cacgcgatgc gcagagtcgc gacacgccgc ggaaatgcac gccatatgcc
     1621 tgtgatcggt gccgcagcag ccgcccagca cgttgatgtg cgggaagcgg cggagcagtt
     1681 cggcataaag atgaccaagt tcgtgcggat cgccggcgtc gagctcggtc gcattgtcga
     1741 gctcggcatg gctcttgcgc gaggcgttcg cgcggagccc gcgcagccgt ttcgtccacg
     1801 cggcatcctg gtccaaggcg tcgctgaaat gatcagggtg cgcgcaattg atcatgtaat
     1861 aggcgggccc ttgattcgtc tcccggtcca cggacgcaat ggctgcggcg aggccctcgc
     1921 cggtcggaag cttgccgtcg gtttcgagcg taaaagaaat cacgcatggc atcccggcgg
     1981 ctttggctgc acgggcgacg ccgatggcct cgttgacatt ggtcagcgtg aaagccgaga
     2041 cgagatcggc gcccgcgtcg gcgaacacac cgatttgcca ggcgtgaaac gcttcggcct
     2101 cccatgggct catcaccgcg ccgggatcgt agccgtcgcc gcggggaccg atgttgccgc
     2161 tgaccacaag ctg
//
LOCUS       DASUQP010000138         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1022657, whole genome shotgun sequence.
ACCESSION   DASUQP010000138 DASUQP010000000
VERSION     DASUQP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022657"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1950
                     /gene="yidC"
                     /locus_tag="VFY74_06150"
     CDS             142..1950
                     /gene="yidC"
                     /locus_tag="VFY74_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX5931316.1"
                     /translation="MDEHNRNFLLAIVLSIGVLFAWQFFFMPTPPQVPGTKPAEQQQQ
                     TEIGPPQPQGGGAPAEGALQPGAAAVALTRDEALAKSPRIAIDTPSLKGSIALKGGRI
                     DDLTLKDYRETVDPSSPQVVLLSPEGGPHAYYAESGFVGGAGGEGLALPAADTLWTAA
                     SQAPLTPDSPVTLTYDNGKGLKFTRTIAIDDKYMFTIDDQVTNSGPEPVALYPYAFVR
                     RHETPPIQGFFILHEGLIGIVDDAGLQEITYPNAIESPEPTRSKSKSGWLGITDKYWA
                     TAVVPEQGKPFDATFLGTKKDGSDLFQTDYVLSTPLSIASGQTEQSQGRVFAGAKEVN
                     VVDGYAETYAIPRFDRLIDWGWFYFLTKPLFFALDYFYKLVGNFGVSILIVTVLIKLL
                     LFPLANKSYVAMSKMKKLAPEMQRIKERYGDDRMRQQQAMMELYKKEKVNPASGCLPI
                     LVQIPIFFALYKVLFITIEMRHAPFFGWIKDLSAPDPTSIFNLFGLIPWEPPAFLLIG
                     IWPLIMGVTMWVQMKLNPAPPDPVQAKIFAWMPVFFTFLLASFPAGLVIYWAWNNLLS
                     VIQQSVIMARQGVEIPLLENLGIKNNKSKAKGEAEG"
     gene            2172..2819
                     /gene="yihA"
                     /locus_tag="VFY74_06155"
     CDS             2172..2819
                     /gene="yihA"
                     /locus_tag="VFY74_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX5931317.1"
                     /translation="MNASPDFTSADLARGEALFRGPCTFVKGVTRVADLPKDGRAEVA
                     FAGRSNVGKSSLINALTGRSSLARVSVTPGRTRELNFFALGKDGALYLVDMPGYGYAR
                     ASKAQAKAWTRLIGDYLKGRRELKRAFLLIDARHGIKPNDTDTMKLLDEAAVSYQVVL
                     TKADKPKASELAEIEAKVASDLAKHPAAYPQILTTSVRTGLGIEALRAAIAALAP"
     gene            complement(2956..>3298)
                     /locus_tag="VFY74_06160"
     CDS             complement(2956..>3298)
                     /locus_tag="VFY74_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931318.1"
                     /translation="PRLFAGIAKSMSQKPEKEGLTTSAAGGPPPARSALPGRGGLARI
                     SAAFWNSLRGLQEGLSTESAIKQEVAIACVLLPLSFFIAKDRYARADFPSPLVGEGFP
                     PGARSAQVAQR"
BASE COUNT          634 a         1088 c          991 g          585 t
ORIGIN      
        1 ttcactgcgt taatcaagga cctcgaacgc gcgtttgcca aggcgcacgc gcccacgcga
       61 gcaaagagac actgaacgcc cacaaatccg actgcacctc ccgcgcgagg ggacaatcgc
      121 gggccttgag ctgaaagcga catggacgag cataaccgca acttcctcct cgccattgtg
      181 ctgtcgatcg gcgtcttgtt cgcctggcag ttcttcttca tgcccacgcc gccgcaggtt
      241 cccggcacga agccggcgga acaacagcag cagaccgaaa tcggaccgcc gcaaccgcaa
      301 ggcggaggcg cgccggccga aggcgcgctg cagccgggtg cggccgctgt tgcgttgacc
      361 cgcgacgagg cgcttgccaa aagcccgcgc atcgccatcg acacgccgag cctcaaaggc
      421 tcgatcgcgc tcaagggcgg gcgcatcgac gatctgacac tcaaggatta ccgcgagacg
      481 gtcgatcctt cgagcccgca agtcgtgctg ctctcgcccg agggcggtcc gcatgcctat
      541 tacgcagagt cgggcttcgt cggcggcgcc ggaggcgaag gcctcgccct gcccgcggcc
      601 gacacgctgt ggacggctgc gagccaagcg ccgctgacgc ccgactcgcc ggtgacgctc
      661 acctacgaca acggcaaggg gctcaaattc acccgcacca tcgcgatcga cgacaaatac
      721 atgttcacga tcgacgacca ggtgaccaat agcgggccag agccggtcgc gctctatcca
      781 tacgcctttg tccggcgcca cgagacccca cccatccagg gtttcttcat cctgcacgag
      841 ggcttgatcg gcattgtcga cgacgccggc ctccaggaga tcacttaccc gaacgcgatc
      901 gagagcccgg agccgacgcg ttccaagagc aagagcggat ggctcgggat caccgacaaa
      961 tactgggcga ccgccgtcgt tccggagcag ggcaagccct tcgacgccac gttcttgggc
     1021 accaagaagg atggcagcga cctgttccag acggattacg ttctgtccac gcccctctcg
     1081 atcgcgtcgg gacaaaccga gcagtcgcaa gggcgcgtgt ttgccggcgc caaggaggtc
     1141 aatgtcgtcg acggctacgc cgagacctac gccatcccga gattcgaccg cctgatcgac
     1201 tggggctggt tctacttcct caccaagccg ctcttcttcg cgctcgacta tttctacaag
     1261 ctggtcggca atttcggcgt gtcgatcctc atcgtcacgg ttctcatcaa gcttcttctg
     1321 ttcccgctcg ccaacaaatc ctacgtggcg atgagcaaga tgaagaagct tgcgcccgag
     1381 atgcagcgca tcaaggagcg ctacggcgac gaccgcatgc gccagcagca ggcaatgatg
     1441 gagctctaca agaaggaaaa ggtgaacccc gcatcgggct gcctgcccat cctggtgcag
     1501 atcccgatct tcttcgcgct ctacaaggtg ctgttcatca ccatcgagat gcggcacgcg
     1561 ccgttcttcg gctggatcaa ggatttgtcg gcgcccgatc ccaccagcat cttcaatctg
     1621 ttcggactca ttccgtggga gccgcccgcg ttcctcctca tcggcatttg gccgctcatc
     1681 atgggcgtca ccatgtgggt gcagatgaag ctcaatccgg cgccgcctga tccggtgcaa
     1741 gcgaagatct tcgcgtggat gccggtgttc ttcaccttcc tgctcgcctc cttccccgcc
     1801 gggcttgtga tctactgggc gtggaacaat ctcctgtcgg tgatccagca atcggtgatc
     1861 atggcgcggc agggcgtgga gattccgctg ctggaaaatc tcggcatcaa gaacaacaag
     1921 agcaaggcaa agggcgaggc cgagggatag cgccgtcgcc ggcgtctctt cacaacccta
     1981 accgagacca actcttcctc gtcatggccg ggcttggccc ggccatccac gacttgaaca
     2041 gcgcaaccct actttccatt ggagttcgcg gcacggatca aaagtgttaa gcccattcgg
     2101 cgcgcttgtg tttaagtctg aaatcgtgga tggccgggcc aagcccggcc atgacaggac
     2161 cgggaatggg tttgaacgcg tcacccgatt tcacatcagc cgatctcgcg agaggcgagg
     2221 cgctgttcag aggcccttgc accttcgtca agggcgtgac gcgcgtcgcc gacttgccga
     2281 aggatggccg cgccgaggtg gccttcgcgg gccgctccaa tgtcggcaag tcgagcctga
     2341 tcaacgcgct caccgggcgc agcagtctcg cgcgggtctc ggtgaccccg ggacggacgc
     2401 gcgagctcaa tttcttcgcg ctcggaaagg acggcgcgct ctatctggtc gacatgccgg
     2461 gctacggcta tgcgcgggcc tcgaaggcgc aagccaaggc gtggacgcgg ttgatcggcg
     2521 attatctcaa aggacgccgc gagctgaaac gcgccttcct tctgatcgac gcgcgccacg
     2581 gaataaagcc caacgacacg gacacgatga agctgctcga cgaggccgcc gtctcttatc
     2641 aggtcgtgct caccaaggcc gacaagccca aagcttccga gcttgcggag attgaagcga
     2701 aggtggcaag cgaccttgcc aagcacccgg cagcctatcc gcaaattctc acgacctcgg
     2761 tgcgcacggg tttgggcatc gaggcgttgc gcgcggcaat cgccgcgctt gcgccctgac
     2821 ccctcctctt tccgctctcc ccttggggag agcacgcgcg cgcaggcgcg cggtgagggg
     2881 ggtaggcgcg gagcggcgtc gaagagaaga acccctcacc tttcccgcca ttcgtcgcgc
     2941 gctcgacttt ttgcgctacc gctgcgctac ttgtgcgctg cgcgcgccgg gcgggaagcc
     3001 ctctcccacg aggggagagg ggaagtccgc acgggcgtac cgatccttcg cgatgaagaa
     3061 cgacagcggc aggaggacgc aggcgatcgc gacttcctgc ttgatcgccg attccgtcga
     3121 aagcccttcc tgaagccccc ggagactgtt ccaaaaggcg gcagaaattc gtgctaaccc
     3181 ccctcgccct ggaagtgccg atcgcgcggg cggtggaccg cccgcagcgg atgtcgtgag
     3241 cccctctttt tctggctttt gactcatcga tttggcaatc cccgcaaaga gtctcgga
//
LOCUS       DASUQP010000139         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_751185,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000139 DASUQP010000000
VERSION     DASUQP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751185"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..724)
                     /locus_tag="VFY74_06165"
     CDS             complement(155..724)
                     /locus_tag="VFY74_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HEX5931319.1"
                     /translation="MTLSRSQSAWIVGAIIAAAGLALYLIGHPLICKCGYLKLWHFDV
                     MSAENSQHLIDWYTPSHLIHGFLFYWGLWLVSRLLPLPFATRLILAVAIEAAWEVVEN
                     TDFVINHYREMTISLDYYGDSVINSVSDILVMVLGFFMAARLPVWLTVVIAVSLELFV
                     GAMIRDNLTLNVLMFVWPLDAVLHWQQGR"
     gene            1090..>2165
                     /locus_tag="VFY74_06170"
     CDS             1090..>2165
                     /locus_tag="VFY74_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5931320.1"
                     /translation="MTQDEALALLKTGASIFLTGQPGSGKTHTVNRYLQWLKQRGIAY
                     AYTASTGIAATHGHGVTIHAWSGIGVRENLSARDFDLLASNRRLTTRIEKTRVLVIDE
                     ISMLPARSLGLVDTVCRHVRGVPSPFGGLQIVLVGDFFQLPPVVRREAQVAMLPHADG
                     EDPFGAEFAHASSAWRDLAPIVCYLSEQHRQSDKPFLDVLAAIRANDCAGIHRQQLAS
                     RQIGQGAPPENCTRLFTHNAAVDEINQQRLAKLSGETHAFSMAAKGEASLVQALKRGC
                     LSPETLHLKQGAAVMFTKNDQGGRYVNGTLGMVESFDAEEGFPVVATRSGKRVVAEPS
                     TWKIDDAGKERASITQVPLRLAWA"
BASE COUNT          410 a          690 c          688 g          377 t
ORIGIN      
        1 ttctgggtcg gctatcacta caatccttat gtgcgcgcct tcggcttcgg tatcaccttc
       61 tatccgaccg tggcggtcta tttctggctc atcctcagga tggtgttcgg catcgagatc
      121 cggatttgaa ccgctctgcc gttccacgcg cggcctatcg tccctgctgc cagtgcagaa
      181 ccgcatcgag cggccacacg aacatcaaga cgttgagcgt gagattgtcc cggatcatgg
      241 cgccgacgaa gagctcgagg ctcaccgcga tcacgacggt gagccacacc ggcagacgcg
      301 ccgccatgaa gaagccgagc accatgacga gaatgtcgct caccgaattg atgacgctgt
      361 cgccgtagta gtcgagggag attgtcatct cgcgatagtg attgatgacg aaatcggtgt
      421 tctcgaccac ctcccacgcc gcttcgatgg cgacggccaa gatgagccgc gtggcgaagg
      481 ggagcggcag aaggcgcgaa acgagccaga gcccccagta gaacagaaag ccgtgaatca
      541 ggtgggaggg cgtgtaccag tcgatcaggt gctgggagtt ctccgcgctc atgacgtcga
      601 aatgccagag cttgagatag ccgcatttgc agatgagcgg atggccgatc aggtagaggg
      661 cgagccccgc agcggcgatg atggcgccga cgatccacgc tgactgagag cgggagagag
      721 tcatgcgggg cattaaggcc ccagaatcgc tccgccgtcc ccttttttgt catcgcccgg
      781 cttgcctgcc ttcgccgaag cgaagctcgg cttcgcgcag gcaggaccgg gcgatccagt
      841 aactccggcc ctccccgtta tgcggcggcg gttttttctg gatgccctga gttcacaaac
      901 gaagtgcaac accccccacc cggcgcgcaa gaggcgcgcc gacctcccca cgagggggag
      961 gtttggagga agatcatctg acgagtgaca agtcggcctg cgggcacgac aactatcgtg
     1021 ttccacttgc tgtctatgac gccggcgact taggctcgct tcggcgccga gccaggggca
     1081 tttgaaagca tgacacagga cgaagcgctc gctctgctca aaaccggcgc gtcgatcttc
     1141 ctgaccgggc agcccggcag cggtaagacg cacaccgtca accgctatct gcaatggctg
     1201 aagcagcgag gcatcgccta cgcctatacc gcctcgaccg gcatcgccgc gacccacggc
     1261 catggcgtga ccatccatgc ctggagtggg atcggcgtgc gggagaacct gagcgcgcgc
     1321 gacttcgacc tgctcgcgtc caatcgccgg ctcacgaccc gcatcgagaa gacgcgcgtg
     1381 ctggtgatcg acgagatctc gatgcttccg gcgcggagcc ttggcttggt cgatacggtt
     1441 tgccggcatg tcaggggcgt cccctcgccg ttcggcggac tgcagatcgt gctggtcggc
     1501 gatttcttcc agcttccgcc ggtcgtgcgg cgtgaggcgc aagtcgcgat gctgccgcat
     1561 gcagacggcg aggatccgtt cggcgcggag ttcgcgcatg cgtcctccgc ctggcgcgac
     1621 ctcgcgccca tcgtctgcta tctgtccgag cagcaccggc agagcgacaa gccgttcctc
     1681 gacgtcctcg ctgcgattcg cgccaacgat tgcgccggca tccatcgcca gcagctcgcc
     1741 tcccgccaga tcgggcaagg cgcgccgccc gaaaattgca cgcggctctt cacccacaac
     1801 gccgcggtcg acgagatcaa tcagcagcgt ctggcgaagc tcagcggcga gacgcatgcg
     1861 ttcagcatgg ccgccaaggg agaggcgtct ctcgtgcaag cgctgaagcg gggctgcctg
     1921 tcgcccgaga cgcttcacct caagcagggc gccgcggtga tgttcaccaa gaacgatcag
     1981 ggcggccgtt atgtcaacgg cacgctcggc atggtcgaga gtttcgatgc agaggaaggc
     2041 tttcctgtgg tcgccacacg gagcgggaag cgcgtcgtcg cagagccctc gacttggaag
     2101 atcgacgacg ccggcaagga gcgggccagc atcacgcaag tgccgctccg cctcgcctgg
     2161 gcaat
//
LOCUS       DASUQP010000140         5408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1704713, whole genome shotgun sequence.
ACCESSION   DASUQP010000140 DASUQP010000000
VERSION     DASUQP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704713"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFY74_06175"
     CDS             <1..832
                     /locus_tag="VFY74_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX5931321.1"
                     /translation="PAAAQDRGESASGIRLAQAEGPMVITPPPYSGQPGSYPSPQQYS
                     GQPGSYPPPQQYSDEPGSYPPPKPYSGQPGSYPPSPSGYDRGGPGGPYEPAPQGGAYV
                     PRSGGYSSQAAGDVSTFSPDEIKNAGHRFFGRVSMGFARAVEYAFQRSGRPNGYILGE
                     EAGGAFVAGLRYGEGTLHTKDAGTHKVYWQGPSVGYDFGAEGSKTMVLVYNLRSPAEI
                     FMTYPGIGGSAYFIGGVGITFLARDHVTLAPIRSGVGLRLGANIGYLKYTREPTWNPF
                     "
     gene            968..2083
                     /locus_tag="VFY74_06180"
     CDS             968..2083
                     /locus_tag="VFY74_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931322.1"
                     /translation="MINALLFGALGFLLGCLLALALAPPLWNRAVKLTTRRLEATMPM
                     SLNDIQADKDQLRAEFAIELRKVEVALDKAREKAARELIESNKRRAEIATLNTDLAGA
                     RGKLQENESANRVLQQTIKRRLPDLDSRLKSAKKALAELESVNAELRTTIASQSDALK
                     TARTTLHNQRSDIDRLRTALESGTGAAIRGLGKPDARTLAESQRLSAELSKAQEELAH
                     ARTSAQESSLLRRELSRLSSQILAVARAQGLAQAGTPQPVVEVAEVFVEQSAVEAPQS
                     EQDWQRAQKGNGAGPVAEANETGDRVDSVAAGREPPGHREQEAADQADEAEDARDESK
                     SFARRFVARREARRARKAGGTTLRDRLRGLAAERPEN"
     gene            complement(2098..2847)
                     /locus_tag="VFY74_06185"
     CDS             complement(2098..2847)
                     /locus_tag="VFY74_06185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5931323.1"
                     /translation="MPLSVPSDLDHPALPEGQLLYAVGDIHGRLDLMRVLLARIEEDA
                     RTRSHAKRRSLVFLGDYVDRGPDSRGVVADLLARQFQGFDTHFLKGNHEAILLDFLDH
                     PLHLDHWLLNGGQATMESYGVDTEALAEARAHPQTWRETFAAALPETHLRFFRTLNLS
                     VTFGDYLFVHAGVRPNVPLAAQSEDDLVWIRRPFLDHAGPFGKIVVHGHTPTALPVVR
                     PNRIGIDTGAVFSDRLTALRLEDRARDFLHT"
     gene            complement(2919..3359)
                     /locus_tag="VFY74_06190"
     CDS             complement(2919..3359)
                     /locus_tag="VFY74_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931324.1"
                     /translation="MPVSPRVGAGLALAGVLIGLAAIQAYSRKLEPVVQSYGTTYYDG
                     AGPAFAPVACKPDSEILSAIEQVHAKYDQFEGASLASFEKRATLLKGFPPLNVEKLYV
                     ITEDDQLRHGEMVLFIGLKHGCVKTVFGLPTKVYREILSAPAGA"
     gene            complement(3396..3824)
                     /locus_tag="VFY74_06195"
     CDS             complement(3396..3824)
                     /locus_tag="VFY74_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931325.1"
                     /translation="MGVHIPPILVVIACKTVLAGPPDQNAAFTNYENREWATEHSMMV
                     CRRHEIQLFDPAEGVQLGPSHDPAPALNPNFALATQCARAGIRLATDWDQSHRNTPWR
                     VWRIGCPSPIVDLRTGTVIGYKLPECGHTDTVICETDSVI"
     gene            4584..>5408
                     /locus_tag="VFY74_06200"
     CDS             4584..>5408
                     /locus_tag="VFY74_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931326.1"
                     /translation="MLRDVSTRRKLFLLCSAFVVSIAVAIYISVAEKRIAIDFASKEL
                     AGVGHIEQFRDIYAALLPDPLSRTSPPPFPEAPVKSRADPKSASQDSLQTAALEESLA
                     RTLRRLWSGQVKGDAKRTLAAEALAKAERVILRIGDDSNLALDPELDSYHLQDILVRQ
                     MPKLLSQLGNVWSLESSSASATTPEEKIRLLTRAAIVRTSTQELARSLTSAYRGNVSG
                     GLRRGTEATMAVMLSSVNAYLAGVDESLAQPGTFDRASLEPLWNSAAEDALTAWTGG"
BASE COUNT         1013 a         1724 c         1734 g          937 t
ORIGIN      
        1 tccggcggcg gcccaagatc gcggcgagtc agcatcgggc attcgcctgg cacaggccga
       61 aggcccgatg gtgattacgc cgccgcccta ttccggccag cccggttcct atccgtcccc
      121 gcagcaatat tccggccagc ccggttccta tccgcccccg cagcaatatt ccgacgaacc
      181 cggttcctat ccgcctccga agccctattc cggccagccc ggttcctacc cgccttcccc
      241 gagcggttat gacagaggcg gtccgggggg tccttacgag ccggcgccgc aaggtggagc
      301 ctatgtgccg aggagcggcg gctacagctc gcaagcagcc ggagacgtga gcacgttctc
      361 cccggatgag atcaaaaacg cggggcatcg cttcttcggc cgggtgtcga tgggatttgc
      421 ccgcgccgtc gaatatgcct tccagcgctc gggacgtccc aacggctaca tcctgggcga
      481 ggaggccggc ggcgccttcg tcgcggggct acgctatggc gagggcacgc tccacaccaa
      541 ggatgccggc acccataagg tctactggca gggcccctct gtcggctacg atttcggtgc
      601 cgagggttcc aagaccatgg tcttggtcta caatctccgc agtcccgcag agatcttcat
      661 gacttatccc ggtatcggtg gttcggccta tttcatcggc ggggtgggca taaccttcct
      721 cgcccgcgat cacgtcacgc tggcgcccat ccgctcgggt gtcggcttga ggctgggggc
      781 caatatcggc tatttgaaat atacgcgtga gccgacctgg aatccgttct agtttggcgt
      841 taagaagcct ggcggaggcc cctttttagc ggttcgcgcc gaattggcgc caattttccg
      901 cgcaattctg ccgaacacgc gccggcttgg tgacgcgcaa cgggcgcgaa tcgggagcgc
      961 tgacaagatg atcaatgcgt tgctatttgg agcgcttggc ttcctgttgg gatgcctgct
     1021 ggcgcttgcg ctcgccccgc cattgtggaa ccgcgccgtc aagctcacca cgcgacggct
     1081 cgaggccacc atgccgatgt ccttgaacga catccaggcg gacaaggacc agctgcgcgc
     1141 cgagttcgcc atcgagttgc gcaaagtcga ggttgcgctc gacaaggcga gagagaaggc
     1201 tgcgcgagag ctgatcgagt ccaataagcg ccgggccgaa atcgccacgc tcaacaccga
     1261 ccttgccggc gcccggggga agttgcagga gaacgagagc gcgaaccgcg tgctgcagca
     1321 gaccatcaag cgccgccttc ccgatctcga cagccggctc aagtccgcca agaaggccct
     1381 ggcggagctc gaatcggtca atgccgagtt gcgcaccacg attgcgtccc agtccgacgc
     1441 gctcaagacc gcgcgcacaa ccctgcacaa tcagcgctcc gacatcgatc gtttgcgcac
     1501 ggcgctggaa agcgggactg gcgccgccat tcgcggattg ggcaagcccg acgcgcgcac
     1561 gctggccgag agccagcgct tgagcgcgga gctgtcaaag gcgcaagagg agctggcaca
     1621 cgcccggacg agcgcgcaag agagcagcct tctccgccgc gagctgagcc ggctctcctc
     1681 gcagatcctt gccgtcgcgc gggcgcaggg tctggcgcaa gcaggaaccc cgcagcccgt
     1741 cgtcgaggtc gccgaggtct tcgtcgagca aagcgcggtc gaggcgccgc aatccgaaca
     1801 agactggcag cgcgcccaga aaggcaacgg cgccggaccc gtggccgaag ctaacgaaac
     1861 cggagatcgt gtcgactccg tagcggccgg gagggagccc ccgggtcacc gtgaacagga
     1921 ggccgcagac caggccgacg aggccgagga cgcgcgcgac gagtcgaaga gttttgcgcg
     1981 aagattcgtc gcgcgaagag aggcacgccg cgcgcgcaaa gcaggcggca cgaccctgcg
     2041 cgataggttg cgaggcctcg cggccgagcg cccggagaat tgaggtcgat aaatccctca
     2101 ggtgtgaaga aagtcccgcg cgcggtcttc tagtctgagc gccgtcaacc ggtcgctgaa
     2161 gacggcaccc gtgtcgatgc cgatgcggtt cggcctgacg accgggagcg ccgtcggcgt
     2221 atgcccgtgc accacgatct tgccgaaggg cccggcgtga tcgaggaagg gacgcctgat
     2281 ccaaaccaga tcgtcttcgg attgcgccgc cagaggtacg ttcggccgca cgccggcatg
     2341 gacgaagaga tagtcgccga aggtgacgct gagattcaaa gtcctgaaaa accgaagatg
     2401 cgtctcgggc agcgccgccg cgaaagtctc gcgccaggtc tgaggatgag cgcgcgcctc
     2461 cgcgagcgcc tccgtatcga cgccgtagga ttccatcgtc gcctgcccgc cattcaacag
     2521 ccaatgatcg agatgcagcg ggtggtcgag gaagtcgagc aggatggcct cgtgattgcc
     2581 cttgaggaaa tgggtgtcga agccttgaaa ctgtcgggcg aggaggtccg cgaccacgcc
     2641 gcggctatcg ggcccgcgat cgacatagtc gccgaggaac acgagggagc gccgcttcgc
     2701 gtgcgagcgc gtgcgcgcat cctcctcgat ccgcgcgagc aagacgcgca tcaagtcgag
     2761 ccgcccgtgg atatcgccca cggcgtagag gagctggcct tccggcagcg ccggatggtc
     2821 caaatctgac gggaccgaga gcggcattgc tgccgccact ctagtccaaa gcggcgcggg
     2881 caagcgtgca atttaggccg ggaggcggtc tccctcgctt aagcgcccgc gggcgcgctc
     2941 aggatctcgc ggtacacctt ggtcgggagc ccgaacaccg tcttcacgca gccatgtttg
     3001 agcccgatga aaagaaccat ctccccgtgg cggagctggt catcctcggt tatgacgtag
     3061 agcttctcga cgttgagggg aggaaaccct ttgagcagcg tggcacgctt ctcgaacgaa
     3121 gcgagagagg cgccttcgaa ttggtcgtat ttggcatgga cctgctcgat ggcggagagg
     3181 atctcgctgt cgggcttgca agccacgggc gcgaaggccg gtcccgcgcc gtcatagtag
     3241 gtcgtgccgt aggactgaac gaccggctcc agcttgcgcg agtaggcttg gatcgcggca
     3301 aggccgatca gcacgccggc aagcgcaagg cctgcgccaa ctctcggcga gacaggcatg
     3361 tcacgtactc cgcccgagat cggaatccgt ctcggctaga tcacggaatc cgtttcgcag
     3421 attacggtgt ctgtgtggcc gcactccggc agcttatagc caattaccgt ccctgtcctc
     3481 agatccacga tcggggacgg gcaaccgatg cgccagaccc gccaaggcgt gttccggtgg
     3541 ctctggtccc aatcggtcgc aaggcgaatt cccgcgcgcg cgcattgcgt ggcgagcgcg
     3601 aaattgggat tgagcgccgg ggcaggatcg tgagaggggc cgagctgcac accctcggcc
     3661 ggatcgaaga gttggatctc gtggcgccgg cacaccatca tggaatgctc ggtcgcccat
     3721 tcgcgattct cgtaattggt gaaggcggcg ttttggtccg gaggaccggc cagtaccgtc
     3781 ttgcaggcaa tgaccacgag aatgggaggg atgtgcacgc ccatggctag gccctccctt
     3841 caattgagat gttgagaagc ttaggcggcg ctgtcggcgg ttgcctcttg gcggtaatgg
     3901 gcgggctcgc cgcgccggca aggatgaggg tcttcagcat cttcagggcc tccaaatgga
     3961 gttgatcggt aagcaccgct tacttctccg gcccttcctc cggaccggac tcttgccctt
     4021 gacgcatcct ccatttcgca acgcgaattt gatcgcttgg ttcagccgac tcggaacgct
     4081 tcaagtttca gtcgactctg aacgcttcaa accctcgcac cggcgatatt gcaatgcaaa
     4141 atccgaatca cgagtccttc taacgtatgc cttaccgcgc aagcaggata gagcagcgcg
     4201 gcaacagtcc taggactttt tgcagcgcgg cgcccatcct ctcggccttt ttttgctgcg
     4261 cttcgcccct ccccttggac catttgccgc actccgctat cgcccgtgac gcgcgcacgg
     4321 cccccggtgc ggctgcccgg ccgcaagagg cacgaagccc acgctcgcgg cggcgaattg
     4381 aagaatctca gcttttcggc cgatttcctt tcgtcgccca cggaattacc gccctatgcc
     4441 gaccgccgac agccgtctag aattcaggga cgggaggcca gatcggggtt cgagggtacc
     4501 agacgtatga agccgtttag ggagcaagca gaggctgcga cctctgccct gctgcgcagt
     4561 ctggccgtgg attcggccaa gtcatgctga gagacgttag cacgcgcaga aagctgttcc
     4621 tgctttgcag cgcgttcgtc gtctctatcg ccgtggcgat ttacatctcc gttgctgaaa
     4681 agcgcattgc catcgatttt gccagcaagg agctcgccgg cgtcggtcat atcgagcagt
     4741 ttcgcgacat ctatgccgcg cttctccccg atcctctcag ccgcacgtcg ccgccgccat
     4801 ttcccgaagc gccagtaaag tcacgcgccg accccaaatc agcgtcacag gacagtctgc
     4861 agaccgcggc actcgaggag tcgctggcga ggacgctgcg cagactttgg tcgggccagg
     4921 taaagggtga tgcaaagcgg acccttgccg ccgaagccct ggccaaggcc gagcgcgtga
     4981 tcttgcgcat cggagacgat tccaatctcg cgctcgatcc cgagctggac agctaccatc
     5041 tgcaagacat cttggtgcgg caaatgccca agctgctcag ccagctcggc aacgtgtgga
     5101 gcctggagag cagttctgcg tcggcgacga cgccggaaga aaagattcgc ctcctgaccc
     5161 gcgccgcgat cgtccgaacg tcgacgcagg agcttgcccg aagtttgacc tccgcctacc
     5221 ggggcaatgt aagcggagga ttgcggcggg gcaccgaagc gacgatggcc gtgatgctgt
     5281 ccagcgtgaa cgcttatctc gccggcgtcg atgagagcct ggcccaaccg ggcacgttcg
     5341 atcgcgcctc gctcgagccg ctgtggaaca gcgcggcgga ggacgcgctg acggcctgga
     5401 cgggcgga
//
LOCUS       DASUQP010000141         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_480358,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000141 DASUQP010000000
VERSION     DASUQP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480358"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFY74_06205"
     CDS             <1..894
                     /locus_tag="VFY74_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HEX5931327.1"
                     /translation="EPTAPPAGGIVTTDEAAQHAAPPQATPTCRRSVIVDATSDLVDF
                     NVNQNAWMSSMLLYKLGLFGIDWDRTFFFDNNAAFQRGVHQAVRRTSIELVDTLGRVR
                     GTSQIDASLQNARGNLQFNEYTWYFSLKPFGPKTPTPSFYRAAIPDFKDFNMRLEKCD
                     AVFDARADNLLQFVDRIANDIGATSAILRDRSEKYNSGWFDPRADDRFWFAYGQLYGY
                     YGILTTAHADFRDVIAQRGLTPIWAELEEQLRASLNIQPAIISNGAESGWLMPAHLAT
                     LGFYVLRVRSNLVEVRLVLDR"
     gene            1047..2168
                     /locus_tag="VFY74_06210"
     CDS             1047..2168
                     /locus_tag="VFY74_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX5931328.1"
                     /translation="MTGWWRWIELSRRSVLGAGGLALAAGAVGIARGDYGDPVPHAPV
                     KTVPAGVASPHHAAHGGMLTVGEVDPARNGFDPTRILTDWDRGRASTLADGRTLREFD
                     IIAEDKEIEIAPGIVFPAWTYNGRVPGPTMRVTEGDRVRIRFANAGSHPHSMHFHGIH
                     AARMDGVPGAGEAVPGESFVYEFDAFPFGCHLYHCHSVPLKRHIHKGLYGVFIVDPDP
                     ARHPEMVEAASARLLASADNAAWQEFVMVMNGFDTNFDEENEVYAANTIAHAYAHKPI
                     PIDRARKVRIYLVNVTEFDPINSFHLHANFFDYYDTGTTLTPTLKTVDTIMQCQAQRG
                     ILEFSYAEHEPGLYMFHAHQSEFVELGWMGFFDVRDAVA"
     gene            2165..3319
                     /locus_tag="VFY74_06215"
     CDS             2165..3319
                     /locus_tag="VFY74_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HEX5931329.1"
                     /translation="MMRAIAQIGGPVAAILMLIAIFFAYEPLRRPFGGTPPPVEALTV
                     ERAVLDENGIALKVRADGTEPVTIAQVQVDGAYWQFSQDPPGARPRLASAWIKIPYPW
                     VEGETHKIVLVTRNGLTFEHEIAVAWPTPKVGATELGRLTLGGLFVGVLPVALGMLFY
                     PALRAGGPLAFRFALALTIGLLAFLLIDMLEEALELAGRAASGLNTALLVWAVAGLTC
                     LLLFGLARAGGAPLEGTALAVSIALAIGLHNLGEGLAIGSAFATGAAALGGYLLLGFT
                     LHNVTEGIGIVAPLLDRRPGLLLLAALAALAGLPAVLGMWLGAFAFSSHFAALALAIG
                     AGAILQVMIEVGLLLGRDLAQGAARFFPITLAGLASGVALMYVTALLVPV"
     gene            3420..3752
                     /locus_tag="VFY74_06220"
     CDS             3420..3752
                     /locus_tag="VFY74_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931330.1"
                     /translation="MVSEAAGGVGARTGEHSAEREIERLLLDAAGALQRLVAERDSLK
                     ARVDAQERELARLQEHVALFHDSYRRLTSEFITQSQLIDSAVSRFVGAKPAENEAFKG
                     EPEARSEG"
     gene            3847..4299
                     /locus_tag="VFY74_06225"
     CDS             3847..4299
                     /locus_tag="VFY74_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931331.1"
                     /translation="MNILGPNPQRGYGPVDWNRLSQKRAEAGEVTPLNLLGVRAEGEE
                     EAPANGGSSDLLIRGLVERLPHTDDVWPLEDRVRWLRTAASIFALVYRTGEGEAGEIG
                     VSLTRVAGSAKAAEAPPTKTPDDAPAKTRDAALTKAPADFSVLGDFSS"
BASE COUNT          744 a         1523 c         1437 g          840 t
ORIGIN      
        1 gagcccaccg ctccgccggc gggcggcatc gtgacgactg acgaggcggc gcagcacgcg
       61 gcgccgccgc aagccacgcc aacctgccgc cgctcggtga tcgtcgatgc gacgagcgac
      121 ctggtcgact tcaacgtcaa ccagaacgct tggatgtcct cgatgctgct ttacaagctc
      181 ggcctgttcg gcatcgactg ggaccgcacc ttcttcttcg acaacaatgc cgcgttccag
      241 cgcggcgtgc accaggcggt gcggcgcacc tcgatcgagc tcgtcgacac gcttggccgc
      301 gtgcgcggca cgtcgcagat cgacgcaagc ctgcagaacg cgcgcggcaa tctgcaattc
      361 aatgaataca cctggtattt cagcctgaag cccttcggcc ccaagacgcc gacgccgagc
      421 ttctatcgcg ccgccatccc ggatttcaaa gacttcaaca tgcggctcga gaaatgcgat
      481 gcggtgttcg atgcacgcgc cgacaatctg ctgcagttcg tcgaccgcat cgccaacgat
      541 atcggcgcga cctcggcgat tctccgggac cggtccgaga aatacaacag cggctggttc
      601 gacccccgcg ccgacgaccg cttctggttc gcttacggcc aactctacgg ctattacggc
      661 atcctcacca ctgcgcatgc cgatttccgc gacgtgatcg cgcagcgcgg gctgactccc
      721 atttgggccg agcttgaaga gcagcttcgc gcctcgctca acattcagcc tgcgatcatc
      781 tcaaacggcg ccgaatcggg ctggctgatg cccgcccatc tcgccacgct cggcttctat
      841 gtcttgcggg tgcgctcaaa cctggtcgag gtccgcctcg tgctggaccg ttagggtcgc
      901 gcgacccgcg gcgtgccggc catcgggctt gcgggcttat gctggaatga cggtcggcta
      961 gcggctttgg cctcgacaga gcctgtcggc tgaattagca tgcaaatgac aatgacttgc
     1021 atctggtttc cgggagtgtc gaggcgatga ccggttggtg gcgctggatc gaactgtcgc
     1081 gtcgctccgt gctgggagcg ggtgggctcg cgcttgccgc cggtgccgtc ggaattgccc
     1141 gtggcgatta tggcgatcca gtcccgcatg cgccggtcaa gaccgttccg gcaggcgtcg
     1201 cttcgcccca ccacgctgcc cacggcggca tgctcacggt gggcgaggtc gaccccgccc
     1261 gcaatggctt cgatccaacc cgcatcttga ccgactggga ccgcgggcgc gcgagcacgc
     1321 tcgccgacgg ccgcaccctc agagagttcg atatcatcgc cgaggacaaa gagatcgaga
     1381 ttgcgcccgg catcgtcttt cccgcatgga cctataacgg ccgcgttccg ggcccgacca
     1441 tgagggtcac agagggcgac cgcgtccgca tccgcttcgc caatgccggc tcgcatccgc
     1501 acagcatgca cttccacggc atccacgccg cacgcatgga cggcgtgccg ggcgcgggcg
     1561 aggccgtacc gggagagagc ttcgtctacg aattcgatgc cttccccttc ggctgccacc
     1621 tttatcattg ccactcggtg ccgctgaagc ggcacatcca taaggggctg tacggcgtct
     1681 tcatcgtcga tcccgatccg gcgcgtcatc ccgagatggt ggaggccgcc tccgcgcggc
     1741 tcttggcctc ggcggacaat gcagcgtggc aggaattcgt catggtgatg aacggcttcg
     1801 acaccaattt cgacgaggag aacgaagtct atgccgccaa taccatcgct catgcctacg
     1861 cgcataagcc gattcccatc gaccgcgcgc gcaaggtgcg catctatctc gtcaacgtca
     1921 ccgagttcga tccgattaac tcgttccatc tgcatgccaa tttcttcgat tattacgaca
     1981 ctggcacgac gcttaccccg acgctgaaga cggtcgatac gatcatgcag tgccaggctc
     2041 agcgcggcat cctcgagttc agctatgccg agcacgagcc cggcctgtac atgttccatg
     2101 cgcatcaatc ggagtttgtc gaactcggct ggatgggatt cttcgacgtc agggacgccg
     2161 tggcatgatg cgcgcgatcg cgcaaatcgg tggaccggtc gccgcgatcc ttatgctcat
     2221 cgccatcttc ttcgcctatg agcctctgcg ccggcccttc ggcggcacgc cgccgccagt
     2281 cgaggcgctg acggtcgagc gagccgtgct cgacgaaaac ggcatcgcgc tcaaagtccg
     2341 tgccgacggg accgagccgg tgacgatcgc gcaagtgcag gtcgatggcg cctattggca
     2401 attctcgcag gatccgccgg gcgcgcgccc tcgtctcgcc agcgcctgga tcaagattcc
     2461 ctacccctgg gtggagggcg agacccacaa gatcgtgctc gtcacccgca acgggctgac
     2521 cttcgagcac gaaatcgcgg tcgcctggcc gacgccgaag gtcggcgcca cggagctcgg
     2581 ccggctgacg ctgggcggcc tgttcgtggg cgtgctcccg gtcgcgctcg gcatgctgtt
     2641 ctatcccgcc ttgcgcgcgg gcggcccgct tgccttccgt tttgcgctgg cgctcaccat
     2701 cggactgctc gccttcctcc tgatcgacat gctcgaagag gcgctcgagc tcgcgggccg
     2761 cgccgcctcc ggtctcaata ccgccctgct ggtctgggcc gtggccgggc tcacctgcct
     2821 ccttctgttc ggattggcgc gggccggcgg cgcgccgctt gagggcacgg cgctcgccgt
     2881 ctcgatcgcg ctcgccatcg ggctccataa tctcggcgag ggactcgcca tcggctcggc
     2941 cttcgccacc ggcgccgccg cgctcggcgg ttatctcctg ctcggcttca ccttgcacaa
     3001 cgtgaccgaa gggatcggca tcgtggcacc cttgctcgac cgccgcccgg ggcttctcct
     3061 gctcgccgct ctcgccgcgc tcgccggcct gccggcggtg ctcggcatgt ggctcggtgc
     3121 gtttgccttc tcgagccatt ttgccgcgct cgctcttgcc attggcgcgg gcgcgatcct
     3181 ccaagtcatg atcgaggtcg gccttctgct gggacgcgat ctcgcccaag gcgctgcgcg
     3241 cttctttccc attacgctcg ccggcctcgc gtcgggcgtg gcgctcatgt acgtcacggc
     3301 gcttctggtc ccggtctagc cgggaccctc gcgcggcgtt aaccagtcat cgcgcggctg
     3361 tgcgcgccgt ggtaaactaa atgcgagcca gacacccgca cagtgaggat gttatcgcca
     3421 tggtgagtga agcggcggga ggagttgggg cgcgaaccgg cgagcacagc gccgagcgtg
     3481 agatcgaacg ccttctgctc gatgcggccg gcgcccttca gcgcctcgtc gccgagcgcg
     3541 actcgctcaa agcccgcgtc gacgcccagg agcgagagct tgcccggctc caggagcacg
     3601 tcgccctgtt ccacgatagc taccgccggc tcacaagcga attcatcact cagtcgcagc
     3661 tcatcgacag cgccgtgagc cgcttcgttg gggcgaaacc ggcggaaaat gaggccttca
     3721 agggggaacc ggaagcccgc tccgagggtt aaggaaaatg cggcagctca gggaggcggg
     3781 gtcgacgtcc gattcccgat cggctaaaac cgcgtgcggg taaccttggc gcggaggggg
     3841 aatcgaatga atattctcgg tccgaaccca caacgtggct atggccctgt ggactggaac
     3901 cgtctcagcc agaagcgggc cgaggcgggt gaagtgacac cgctcaacct tctcggcgtg
     3961 cgcgccgagg gcgaagagga agcgcccgcc aatggcggga gctcggatct tcttattcgc
     4021 gggctggtgg aacgtctgcc gcacactgac gacgtctggc cgctcgagga tcgggtcagg
     4081 tggttgcgaa cggcggcctc gatcttcgcc ctggtctatc ggacaggcga gggcgaagcg
     4141 ggcgagatcg gcgtgtctct gacgagggtg gccggttctg cgaaggcggc ggaggctccg
     4201 ccaacgaaga cgccggacga cgcgccggcg aagacacgtg acgccgcgct caccaaagct
     4261 cctgcggatt tttcggtctt gggcgatttt tcttcgtagc gagacgcccc gccctaatag
     4321 gccagagcag cagcaccgtc cttcttgcct ggtgatcgtc cgaccgattc ttcgccgccg
     4381 gcgatcggct cgcccatagg tgtgcgggag ggttgcactt agtcccgtgt acgattcata
     4441 gtcttccgag agagggcagg gagatggctg agaaagtcac cagcctgaag ctgaccggct
     4501 tcgaaatcga ggtcgaagac gatcccgtga ccttgctgtt gcag
//
LOCUS       DASUQP010000142         5405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1705811, whole genome shotgun sequence.
ACCESSION   DASUQP010000142 DASUQP010000000
VERSION     DASUQP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705811"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFY74_06230"
     CDS             <1..374
                     /locus_tag="VFY74_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5931332.1"
                     /translation="MKRAAIVPHGSARARFILRLAALFIGSSQLALAQAEEGLTNPII
                     GQPAAISAGRDIYRCKCLVCHHSAGARGPNIFATKLSDHQFAETVIHGRKGTQMPGFG
                     HRLSLEEVWQVHAFVKSTDHY"
     gene            548..1789
                     /locus_tag="VFY74_06235"
     CDS             548..1789
                     /locus_tag="VFY74_06235"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX5931333.1"
                     /translation="MLYMGRRAREAAATLALASTEAKNAALLAMAEHLERSASKILEA
                     NSQDLEAARGKGRDAAFLDRLALTASGVASMANGLRDIAAVADPVGQVTARWTRPNGL
                     EISRIRVPLGVVGIIYESRPNVTADAAGLCLKSGNAAILRSGSESFLTSLVIANVLGE
                     ALAAAGLPEASIQLVPTVDRSAVGFMLGGLAGAIDVIVPRGGKDLVGRVQAEARLPVF
                     AHLEGVCHVYVHGSADPDMAKKIVLNAKMRRTGICGAAETLLVDRAAAPQQLAPLVKM
                     LIGAGCEVRGDPATQAVDTRVRPATEADWPKEYLDAIIAVKVVDSLDEAVAHIARYGS
                     QHTDAIVTEDRKAAEEFLARVDSAIVLHNASTQFADGGEFGMGAEIGIATGRFHARGP
                     VGVEQLTIFKYAVRGQGQTRP"
     gene            1837..2442
                     /locus_tag="VFY74_06240"
     CDS             1837..2442
                     /locus_tag="VFY74_06240"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5931334.1"
                     /translation="MAAPGMRIGLLGGSFNPPHEAHRAISLAALKRLGLDRVWWVVSP
                     PNPLKDPSTLPSLEARLESARKIARHPRIEVTGFSGGSAYTVDLLVALKRRFPGVQFV
                     WLMGADNLAELHRWHAWEEIFARVPIAVFDRPGYRLKARASRAAQRFLDYRVDETDAG
                     GLALLIPPAWTMLTHPLSGLSSTALRASATAKKAEKKTKSH"
     gene            2581..2991
                     /gene="rsfS"
                     /locus_tag="VFY74_06245"
     CDS             2581..2991
                     /gene="rsfS"
                     /locus_tag="VFY74_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5931335.1"
                     /translation="MRSAREGAQRGTPPNGFAPFRAHPNRLLNVVLDTLDAAKAEDVA
                     VIDLRGKTSIGDQMVIASGRSQRHVGAVADHLIRKLKDEGFGRARVEGMPHCDWVLID
                     SGDVIVHIFRPEVRTFYNLEKMWSADRPMERLVI"
     gene            3044..3526
                     /gene="rlmH"
                     /locus_tag="VFY74_06250"
     CDS             3044..3526
                     /gene="rlmH"
                     /locus_tag="VFY74_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5931336.1"
                     /translation="MRLLIAAVGKLKQGPERELFAHYLGRAEAAGRKLHLSPIAVVEV
                     PESKAATARARKEAEAQALLVKVPSSHRLIALDREGEALSSEAFAALLAKLRDAGAEG
                     VAFLVGGADGLSPEVPAKAAKAISFGALTLPHGLVRVILAEQIYRAATILAGHPYHRG
                     "
     gene            3756..5030
                     /locus_tag="VFY74_06255"
     CDS             3756..5030
                     /locus_tag="VFY74_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX5931337.1"
                     /translation="MALAQDGLSRDEAKQRLDQTEQSLQSNRAKEEGLAKELATLAVE
                     RAKLNSELIAAGGRVQASEAKLSETEAKLGDLTEQVNVIQSSITERKEAIVKMLSAMQ
                     RIGRTPPPALVTRRDDAVAVVRSAMLLAEVFPELKYQADNLSRELEGLVGLESGIREQ
                     RDAEKRETERLTAERAALDRLLEEKSRATAQGEAELADVRRVAVEQAQAVTDLNELIQ
                     QLDASIAKVEIAQYDAEIATERALRSRAQQQALATPGNESVVEIKPQSTRVAFASPDR
                     LKPALPFEAAKGSLRLPAQGQKVKRFGETDAAGATLKGISLQTRVEARITAPADGWVV
                     YAGAFRSYGQLLIINAGGGYHILLAGMSRIDVGLGQFVLAGEPIAEMGNAAGSSRGND
                     NSRPVLYVEFRKDGTPIDPDPWWAEASEKVQG"
     gene            5030..>5405
                     /locus_tag="VFY74_06260"
     CDS             5030..>5405
                     /locus_tag="VFY74_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEX5931338.1"
                     /translation="MRKSEYALWALLVLAVLAAGSTMVSLARSQSASAANSEIYRQLD
                     LFGEVLERVRGDYVEKPEDPKLIEAAINGMLTALDPHSAYLNPKHFRDMQVQTRGEFG
                     GLGIEVTMEDGLIKVVSPIDDTP"
BASE COUNT          992 a         1758 c         1752 g          903 t
ORIGIN      
        1 tcatgaagcg agccgcgatt gtgcctcatg ggtcagcgcg cgctcgcttc atcctccgcc
       61 tcgcagcttt gttcatcggc tcgtcgcagc ttgcgctcgc gcaggcagag gaggggctga
      121 ccaatcccat catcggccag cctgcggcca tctcggccgg tcgcgatatc tatcgatgca
      181 agtgcctggt ctgtcatcac agcgccggtg cccgcggacc gaacatcttc gcgacgaaac
      241 tcagcgatca ccaattcgcc gaaacggtga tccacggccg gaaagggacg cagatgcccg
      301 gctttgggca tcgattgtct ctcgaagagg tttggcaggt gcacgccttc gtcaagtcca
      361 ccgaccatta ttaggggtcc ctgccctgat gcccacgagc aaggagccag tggcgaggct
      421 ggcgcgcagg aacgagtctc tgataacact gtgactgtca aacgcattgg cgatcggtga
      481 gccggacgca accacactgg cacaaaaatt gaaccaggat agagacttag ccacgatcga
      541 gccgatgatg ctctatatgg ggcgccgtgc gcgggaagcc gcggcgacgc ttgcgctcgc
      601 ctcgaccgag gccaagaacg ccgcgctcct cgccatggcc gagcatctcg agcgatccgc
      661 ctccaaaatt ctcgaagcga actcccaaga cctcgaggcg gcgcgtggaa aagggcggga
      721 cgccgcgttc ctcgaccgcc ttgccctgac ggcgtccggc gtcgcttcca tggcgaacgg
      781 cttgcgcgac atcgccgcgg tcgccgatcc cgtcggccaa gtgacggcgc gctggacccg
      841 acccaacggt ctggaaattt cgcgcatccg cgtaccgctc ggcgtggtcg gcatcatcta
      901 cgagtcgcgt cccaacgtga cggcggatgc cgctggactt tgcttgaagt cgggcaacgc
      961 cgcgatcctc aggagcggga gcgagagctt cctcacctcg ctcgtgatcg ccaatgtctt
     1021 gggcgaggcg ctcgctgctg cgggactgcc tgaagcttcg atccagctcg tgccgacggt
     1081 cgaccgctcg gccgtcggct tcatgctcgg tgggctcgcg ggcgcgatcg acgtgatcgt
     1141 gccgcgcggc ggcaaggatc tcgtcgggcg cgtgcaggcc gaagcgcggc tccccgtctt
     1201 cgcgcatctc gaaggcgtgt gccatgtcta tgtgcacgga agcgccgatc ccgacatggc
     1261 aaagaagatc gtgctcaacg ccaagatgcg tcgcaccgga atttgcgggg cggccgagac
     1321 gcttctcgtc gatcgcgccg cggcacccca gcagctcgcg ccgctcgtca agatgctgat
     1381 cggagcgggg tgcgaggtga ggggcgatcc cgcgacgcaa gcggtcgaca cccgcgtgag
     1441 gcctgcaacc gaagccgact ggccgaagga atatctcgac gccatcatcg cggtgaaggt
     1501 tgtcgatagc ctcgacgagg cggtcgcgca tatcgcccgc tacggctcgc aacacaccga
     1561 cgcgatcgtg accgaggacc gcaaagcggc ggaggaattt ctcgcccgcg tcgacagcgc
     1621 gatcgtcctg cacaacgcct cgacccagtt cgccgatggc ggcgagttcg gcatgggggc
     1681 cgagatcggc atcgccaccg gccgtttcca cgcgcgagga ccggtcggcg tcgagcagct
     1741 cacgatcttc aaatatgccg tgcgcgggca gggccagacc cgcccttgag aagcggcgcg
     1801 acaaagacac ggaaaagaga gagcgggcgg ctgccgatgg cggcgcccgg catgcgcatc
     1861 gggcttctcg gcggctcgtt caatccgccg catgaggccc atcgcgcgat cagtctcgcg
     1921 gcgctgaagc gcctcggcct cgaccgggtc tggtgggtgg tcagcccgcc aaacccgctg
     1981 aaagacccgt ccacgctccc gagcctcgaa gcccgcctcg aatccgcgcg caagatcgcg
     2041 cgtcatccgc gcatcgaggt gaccggcttc agcggcggct cggcctacac ggtcgatctt
     2101 ctcgtcgcgc tcaagcgccg cttcccggga gtccagttcg tgtggctcat gggcgccgac
     2161 aatcttgcgg agctccatcg ctggcacgca tgggaggaga ttttcgcacg cgtgccgatt
     2221 gccgtgttcg accggccggg ctatcggctg aaggctcggg cgagccgggc ggcgcagcgt
     2281 ttcctcgact atcgggtcga cgagacggac gcgggcggct tggccctcct cattcccccc
     2341 gcctggacca tgctcaccca tcccctgtcg ggcctgtcct ccaccgctct ccgcgcgagc
     2401 gccacagcaa aaaaagccga gaaaaaaacc aagagccact agccctattg aatctcctat
     2461 ggcgcccata ctatttttat gtcgcgaggc agcgtccgac tcaccccgtc atgcgtttag
     2521 catgcgtatc ggctaggctg tttcgccgag gagcaatggc agaaggaccg tcaccacaac
     2581 atgcgctcag ctagagaggg tgcccagcgc ggcacgcctc ctaacggatt cgcgccattt
     2641 cgcgcgcatc cgaaccgtct cctcaatgtc gtgctcgaca cgctcgacgc cgccaaggcg
     2701 gaggatgtcg cggtcatcga cttgagaggc aagacctcga tcggcgatca gatggtgatc
     2761 gcctcgggcc gctcgcaaag gcatgtcggg gccgtcgccg accacctgat cagaaagctg
     2821 aaggatgagg gctttggccg cgcccgcgtc gagggtatgc cccattgcga ttgggtgctg
     2881 atcgactcgg gcgacgtcat cgtccatatc tttcgccccg aggttcgcac gttctataac
     2941 ctcgagaaga tgtggtcagc cgaccggccc atggagcggc tggtgatctg atctctcgtt
     3001 ccgggcgcct cgctgtcaag gcgagccttt agggcgcata cgcatgcggc tcctgatcgc
     3061 tgccgtcggc aagctgaagc aggggccgga gcgcgagctg ttcgcgcatt atctcggccg
     3121 cgccgaggcc gccggccgga agctccatct ctcgccgatc gccgtggtcg aagtgccgga
     3181 gtcgaaggcc gcgacggccc gggcccgtaa agaagctgaa gcgcaagcgc ttctcgtcaa
     3241 agtcccgtca tcgcatcggc tcatcgcgct cgaccgggag ggcgaggcgt tgtcgagcga
     3301 ggcgttcgcc gcgctcctgg caaaactgcg cgacgcgggc gcagaagggg tggcctttct
     3361 tgtcggtggc gccgacggct tgtccccaga ggtgccggcc aaggccgcga aggcgatttc
     3421 gtttggcgct ctgacgctac cccatgggct tgttcgcgtt atactcgccg agcagatcta
     3481 ccgcgcggcg acgattctcg cgggccaccc ctatcaccgc ggttgagcat gatcgccgag
     3541 acgcgtgaat tttcgggaca gcgaaccgta tgaaaaattt aagactttga ggcactcttt
     3601 tgccttccga tgccttgatt ccgccgtacc gctccgttgt cgttaaagcg gctgaatggc
     3661 caaggcgcat ggcgcatcga ttcgatttgg cttccttacg acaaaggttt cgatccgcgc
     3721 tgcttgcggc tggcctgatc ggcgcttcgc cgggtttggc tctggcacag gacggtcttt
     3781 cacgcgacga ggcgaagcaa aggctcgacc agaccgagca aagcctccag tcgaaccggg
     3841 ccaaggaaga aggcctcgcc aaggaattgg caaccttggc ggtggagagg gcaaagctaa
     3901 acagcgagct gattgcggcg ggcggacgag tgcaggcgag cgaggccaag ctgtcggaga
     3961 cggaagccaa gcttggcgat ttgaccgagc aggtcaacgt catccagagc tcgatcaccg
     4021 agcggaagga agccatcgtc aagatgctga gcgccatgca gaggatcggc cgcacgccgc
     4081 cgcccgcgct cgtgacccgc agggacgacg ccgtcgccgt cgtgcgcagc gccatgctgc
     4141 tcgccgaagt gttccccgag ctcaaatacc aggccgacaa tctctcgcgc gaactcgagg
     4201 ggctggttgg cctcgagagc ggcattcgcg agcagcgcga cgccgaaaaa cgtgagaccg
     4261 agcggctcac cgccgagcgc gcggcgctgg accggctgtt ggaggagaag agccgcgcga
     4321 cggcgcaagg cgaggccgaa cttgccgatg tccgccgggt cgctgtcgag caagcgcaag
     4381 ccgtcaccga tctgaacgaa ctcatccagc agctcgatgc cagcatcgcc aaggtcgaga
     4441 tcgcccaata tgacgcggag atcgccaccg agcgggcgct caggtcgcgg gcacagcagc
     4501 aggccctcgc cactcccggc aacgaaagcg tcgtcgaaat caagccgcaa tcgaccaggg
     4561 tggcgtttgc gagccccgat cggctcaagc cggcgctccc cttcgaggcg gccaaaggca
     4621 gcctgcgcct gccggcccag gggcagaaag tgaagcgttt cggcgaaacc gacgcggccg
     4681 gcgccacgct caaggggatc tcgctgcaga cgcgcgtcga ggcgcggatt accgcccccg
     4741 ccgatggctg ggtggtctac gccggcgcct tccgctcgta cgggcagcta ttgatcatca
     4801 acgcgggcgg aggctatcat attctgcttg cgggaatgag ccgcatagac gttggcctcg
     4861 gtcaattcgt cctcgccgga gaacccatcg ccgagatggg caatgcggcg gggtcgagcc
     4921 gcggcaatga caattcgcgc cccgtcctct atgtcgagtt cagaaaggac ggcacgccaa
     4981 tagaccctga cccatggtgg gccgaggctt ccgaaaaggt gcaaggatga tgcggaaatc
     5041 cgaatacgcg ctttgggcgc tcctggtgct ggccgtgctc gctgccggct cgacgatggt
     5101 gagcctggcg cgcagccagt cggcctcggc cgccaactcg gagatctacc ggcagctcga
     5161 cctgttcggt gaggtgcttg agcgcgtccg cggcgactac gtcgagaagc ccgaagatcc
     5221 caagctgatc gaggcggcga tcaacggcat gctgaccgcg ctcgatcccc attcggccta
     5281 cctcaatccg aagcatttcc gcgacatgca ggtgcagacc cgcggcgaat tcggcggtct
     5341 cggcatcgag gtcacgatgg aagacggcct gatcaaggtg gtctcgccga tcgacgatac
     5401 cccgg
//
LOCUS       DASUQP010000143        20280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_71159,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000143 DASUQP010000000
VERSION     DASUQP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 20280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20280
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71159"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..575)
                     /locus_tag="VFY74_06265"
     CDS             complement(408..575)
                     /locus_tag="VFY74_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931339.1"
                     /translation="MPATSGISKGAQASTTPLVGWAQAITSRPSSGAGPGGMKMAPET
                     AIGSPFSPVER"
     gene            574..1395
                     /locus_tag="VFY74_06270"
     CDS             574..1395
                     /locus_tag="VFY74_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5931340.1"
                     /translation="MIWGLEDMLGQGHVVVATDYPGLGVAGQIHPYLIGVSEGRSVID
                     SVRAARDLPRSGASNRFVVWGHSQGGHAALYAGELAASYAPDLKLLGVATAAPATYLA
                     ELFEADISTPIGKEMTAMALYSWTKLYNKPASTLIESAAMAPFQELAHECVESVLELA
                     ALGRAEKPLEWTRFLKVDPTEIAYWREIMERNTPGQAPAGAPVFIAQGTVDTTVRPDV
                     TKKFAAALCRQGERVRLISLNGVGHIFAAKHSVGQALAWMGARFRGLPAPSDCES"
     gene            complement(1423..1848)
                     /locus_tag="VFY74_06275"
     CDS             complement(1423..1848)
                     /locus_tag="VFY74_06275"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5931341.1"
                     /translation="MRRTVLLAVLFGFILAVPTLAEAQIVTKINLSSQRMAVIVDGKA
                     RYNWPISTARRGYRTPTGTFKPTALVRYHRSTLYDGSPMPHSIFFLRGYAIHGSYEIK
                     HLGRPASHGCIRLHPANAAALYSLVQRRGTGNTLIKIGY"
     gene            2090..3247
                     /gene="mepA"
                     /locus_tag="VFY74_06280"
     CDS             2090..3247
                     /gene="mepA"
                     /locus_tag="VFY74_06280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX5931342.1"
                     /translation="MLRLFFGLLLIIYGGAAAAQQSLAPQTVAAPKPAKPSTKQTAKK
                     KPPKPKSPIARELFGAASEPAPLAARSIGGYAKGCLAGGVSLPINGPDWQVMRLSRNR
                     NWGNPRLVDYLERLASDAHALDGWPGLLLGDMSQPRGGPMLTGHTSHQVGLDADIWLT
                     PMPDRILTPDEREDMTAVSMLKDPFSVDPAIFTLLHVKLIKRAASYPQVARIFVHPAI
                     KKALCQEAPKAGKDLAWLGKVRPWWNHHYHFHVRLSCPPGAEGCDNQKPIAGDNGCGQ
                     ELTNWYAMLKKAAIARGTSPKPGAKPWTGKPPLTMAQLPKECGVVLRAGGFEPPIPAE
                     DGTTEKPPAALAALATKDAGPPIPILTEAQLKALTGENAGGMPLPDRNPLR"
     gene            3287..3682
                     /locus_tag="VFY74_06285"
     CDS             3287..3682
                     /locus_tag="VFY74_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEX5931343.1"
                     /translation="MQERRQEPLAGQRNVYGEPLKACSERPLTGFFRTGCCHTGPEDL
                     GLHTVCVEVTAEFLAFSKARGNDLSTPQPGFGFPGLMPGDRWCLCAARWREAFEAGRA
                     PRVVLGATHEATLEIVDFEDLKRYAIDLA"
     gene            3740..4315
                     /locus_tag="VFY74_06290"
     CDS             3740..4315
                     /locus_tag="VFY74_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5931344.1"
                     /translation="MPKKKPKKKPRASTKQSEPATQVSARKKRQIFAEGHKRKFLVVV
                     DETPECESALAFAASRAQRTGGQLALLYVIEPDVDFQWLAVEDVAREEGHSKAKAVFR
                     LFGRKLKAMGFEALVPEEIVREGMKSEEITRAIEEDEDIGVLVLGASKDPSGPGPLVT
                     DLAGSGLGATFPVPITVVPGHLSTEEILALA"
     gene            4425..6194
                     /locus_tag="VFY74_06295"
     CDS             4425..6194
                     /locus_tag="VFY74_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5931345.1"
                     /translation="MRIAALGLLAAACFSVPAFAAEPNGPAGLITPAEGLRITVQGLA
                     SAKSNSPQRDALVQYYGAPDRNLLWVDDNGLTERAKLVMAEIGKADDYGLRASDYPLP
                     DAASFNPSDPGARDWLAQSEMKVSYAVLDYAKDARGGRIEPVRVSKNLDPNLALPNPS
                     EVLEVIAIRSDPAAYLRSFQPAQPQFEALRQKLIELRGGKVEEVKPNAVVIPAGPVLK
                     FGMEHEQVALLRKRLDVPAGSNENKFDEAVLDAVRQFQSGNGTRPDGAVGAGTRRLLN
                     HGQQPRAVGSEARIRQVLVNMERWRWLPNDLGPFYVNVNIPEFTLRVAEDGKPIHTAR
                     VVVGKPRTQTPVISDELEVIVFNPYWNVPNSIKNEEIAPYMGAGGGFFGGGWDTSVLK
                     RHGLRIKYGNRDIDPDSIDWSRNDIRNFDLVQPPGPSNVLGRVKFLFPNSHDVYMHDT
                     TQKFLFNNAVRAESHGCMRVQNPNLLAQAILKRDKGWSEARVESTFDAGDDHQVALDQ
                     KIPVYVTYFTLRVNEDGTFTSFNDLYGHDSRMTAALEGRGYQSDPVYEEEQVFAGEWR
                     QNQRRVGGRGSFGSDFTRSLFGF"
     gene            6317..6877
                     /locus_tag="VFY74_06300"
     CDS             6317..6877
                     /locus_tag="VFY74_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX5931346.1"
                     /translation="MFIQTEATPNPATLKFIPGRPVLAQGTLEFLDPETSKASPLAAR
                     LFEIEGVKGVFFGTDFISVTKGEGEWQHLKPAILGVIMEHYLSGAAVTDAPEEAEAGE
                     PNEFFAREDEETVKTIKELLETRVRPAVAQDGGDITFQGFRDGVVYLHMRGACSGCPS
                     STATLRHGIENLLRHFVPEVQEVRPV"
     gene            6874..7464
                     /locus_tag="VFY74_06305"
     CDS             6874..7464
                     /locus_tag="VFY74_06305"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX5931347.1"
                     /translation="MTDQLPDSCLDQLFRTARTHNAWRERDVPDALLHEIVDLAKLGP
                     TSANCSPARLLFVKSRAAKERLRPHLSEGNRDKTMKAPVCVIIGYDLDFYIHLPKLFP
                     HTDAKSWFEGKKEKIFDNAFRNGTLQGAYLIMAARALGLDCGPMSGFDNKGVDKEFFA
                     GTNIKSNFLCNLGYGDASVLFPRSPRFEFDEMAQIL"
     gene            7474..8166
                     /gene="tsaB"
                     /locus_tag="VFY74_06310"
     CDS             7474..8166
                     /gene="tsaB"
                     /locus_tag="VFY74_06310"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX5931348.1"
                     /translation="MNILALETSMGACSAALFRRDGGACTVFARTEKMARGHAEALMP
                     MVAQIMSESGTAFAALDRIVATIGPGSFTGVRIAISAARGLALVTGAALFGIDSLTVM
                     AKEALTLGVAGAAPFAVAVDARRGMLYLGLYDGAARRREGPLLIAPDDAANLLPSGLR
                     TAVGSGAARLAEAAVSHGVHIEAKLLDLEPSASSLAALAEESGETALTLRPLYLRPPD
                     ARPQTQASVARC"
     gene            8160..8621
                     /locus_tag="VFY74_06315"
     CDS             8160..8621
                     /locus_tag="VFY74_06315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5931349.1"
                     /translation="MLTTVELKVRQAGPLDADVLAALHRDCFARAWDEAAMIKFAASP
                     ETLCLIGAVAAGASSVAGGLLIARKAGDEADILTFGVVPSYRNRGLGRALLGTATERL
                     RASGTKRLFLEVEESNDAALRLYRSFGGEPVGRRQGYYDDGADAVIFSLAL"
     gene            8675..9112
                     /locus_tag="VFY74_06320"
     CDS             8675..9112
                     /locus_tag="VFY74_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5931350.1"
                     /translation="MRKRAMRESRLERLCAEKGMRMTGQRRTIAKVLSEADDHPDVEE
                     VYRRASARDPHISLSTVYRTVRLFEGAGILERHDFGDGRARYEPAGPGHHDHLINVKT
                     GEVIEFSNEQIERLQENVARELGFRLIGHRLELFGMPLKNGKS"
     gene            9150..9962
                     /locus_tag="VFY74_06325"
     CDS             9150..9962
                     /locus_tag="VFY74_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5931351.1"
                     /translation="MRSLRGFLVLGVFFAFTLPLMPLQLLFLSIGSRHARTFPHWYHR
                     QVCKIVGIRLNIEGKIAEGHGVLLISNHVSWIDISVLSAVAPVSFVAKQEVASWPFVS
                     WLAKLQRSVFIDRTRRSEVGEKANEILNRLAAGDHIVLFAEGTSSDGNSVIPFKTSLF
                     AAAKPPGGAPMGAKVSAQTLALTYNKVYGLPLCRRDRHLIAWYGEMDLATHAWRLLGL
                     GPIDAHIRIGPPMPLDQFPDRKALARYAEDKVRNDVAELLRGRKDAEAAPSH"
     gene            complement(10027..10617)
                     /locus_tag="VFY74_06330"
     CDS             complement(10027..10617)
                     /locus_tag="VFY74_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931352.1"
                     /translation="MRDIRGDLQDRATLLEGEINAQESQFDRLYEQLKQEHETRLEEL
                     KAELEAVNRLMELERRRLEASAAGQRPLVHEAASQAVHEPHQHVQPQPQPQPLHQHVQ
                     PQPQPQPQPPAPRQPQQPLADFLIRKLNEAGAMSRDDLRRFALQEGYFVDAETADRGV
                     HATLINVVKAGLIRQLPNGNFAPATVLDTIRLRRAM"
     gene            complement(10978..12213)
                     /locus_tag="VFY74_06335"
     CDS             complement(10978..12213)
                     /locus_tag="VFY74_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5931353.1"
                     /translation="MAIKTGETTGASAAQQTAIAVLAAISICHLLNDVIQSLIVALYP
                     MIKDSLGLDFRQIGLITFTFTFTASVLQPLVGFVTDRYPTPYSLVAGMGCSLLGLLIA
                     AFASTYGLVLVAVGLIGMGSSVFHPESSRVARLASGGQHGLAQSVFQVGGNFGTALGP
                     LLAAFIVLPFGQQSLAWFSIVALIGMVMLAAIGSWYGRTLKFNSTAHPSEREAIHELT
                     HGKVRKSVAILMALTFSKDFYLVSITSFYTFYLIHTFGLSVQNAQLYLFLFLFAVAAG
                     TVIGGPIGDRIGTRRVIWWSILGVLPFTLALPYVGLLGTAILSVIIGLILASAHPAII
                     VYAQTLIPGRVGMVAGLFFGLAFGVAGIGAAFLGWLADATSITLVYHVCAFLPAIGLL
                     AAFLPEMAKPKRATARHRR"
     gene            complement(12317..13048)
                     /locus_tag="VFY74_06340"
     CDS             complement(12317..13048)
                     /locus_tag="VFY74_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5931354.1"
                     /translation="MITLYGFGPAFGLPDPSPFVMKAEILLKMAGVPYAVQTGGLSKA
                     PKGKLPYVVDDGEPIGDSTFIRWHLEKKYNIDFDRGLNAEQRAVAWAFEKMLEDNLYW
                     AVVHDRWMDDANFAKGPRNFFRAVPAPIRPLVVAMIRRQVRQRLHGQGMGRHSSEEIT
                     ALATRSIDATADFLGTKPYMMGAEPTGVDATSFAFVAGVLCPVFETPIRLAAERHDNL
                     RGYADRMMARFYPGLSLPVGRQTAA"
     gene            13341..13694
                     /locus_tag="VFY74_06345"
     CDS             13341..13694
                     /locus_tag="VFY74_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931355.1"
                     /translation="MAVEDAAKPDPSATGAEAGQLQTQSGVSEDAAKKATEPAEGSAD
                     TSGGAMERSSAPPSSDAAATAKEDASKEAQSSDTSGGAKEQSSAPEGSSAADKSKPNP
                     TIGSETPKEDDTSKE"
     gene            complement(13821..13910)
                     /locus_tag="VFY74_06350"
     tRNA            complement(13821..13910)
                     /locus_tag="VFY74_06350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13874..13876),aa:Ser,seq:gga)
     gene            14088..14405
                     /locus_tag="VFY74_06355"
     CDS             14088..14405
                     /locus_tag="VFY74_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931356.1"
                     /translation="MRTLLRIVGVWLLLLAMVAAVIDATKSLAGGGAWVVTPMGQQWQ
                     ALSPETLRDAKAAIESKVSPFLWDPIMATILDAPTWVVFGILGVLLYWLGQKRKPVEV
                     FIN"
     gene            14446..15105
                     /gene="msrA"
                     /locus_tag="VFY74_06360"
     CDS             14446..15105
                     /gene="msrA"
                     /locus_tag="VFY74_06360"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5931357.1"
                     /translation="MFTLKRKPLSMPSPAEALPGREKPIATAKTHFVNGAALKGPYPQ
                     GFETALFGLGCFWGAERTFWQLPGVHVTAVGYAGGLTPNPTYEEVCSGRTGHNEAVLV
                     VYDPKRVSYDELLKVFWESHDPTQGMRQGNDVGTQYRSGIYYYSEPQRSAAEASKTAY
                     EAALKARGLGPITTEIVPAPEFYFAEDYHQQYLAKNPHGYCGLGGTGVSCPIGTGVGA
                     A"
     gene            complement(15399..16442)
                     /gene="trpS"
                     /locus_tag="VFY74_06365"
     CDS             complement(15399..16442)
                     /gene="trpS"
                     /locus_tag="VFY74_06365"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5931358.1"
                     /translation="MQRFKTKTRVFSGIQPTGNLHLGNYLGAITKFVALQAAHDCIYC
                     VVDLHAITVFQDPEELPKNTREVTAAFIASGIDPARHIVFNQSQVSGHAELAWIFNCV
                     TRLGWLNRMTQFKEKAGKDRENASSGLYVYPNLMAADILLYHATHVPVGDDQKQHLEL
                     ARDVAQKFNNDFAATIAREGYPGGFFPLPEPLITGPATRVMSLRDGTRKMSKSDESDM
                     TRINLTDDEDAIAKKIKKAKTDPEPLPSEVKGLAERPEADNLVGIYAGLADTTKEAVL
                     RDFGNAPFSRFKEALAALAVARLTPIGAEMRRLMSDPVEIDRILADGARRARAIADPI
                     LAKTKDIVGFIRS"
     gene            complement(16511..18064)
                     /gene="murJ"
                     /locus_tag="VFY74_06370"
     CDS             complement(16511..18064)
                     /gene="murJ"
                     /locus_tag="VFY74_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5931359.1"
                     /translation="MQLVRGFATVGTITIASRILGFVRDILIAAVLGASTVADAFFVA
                     FRIPNMFRRLFAEGAFDSAFIPLFSKRIHADPEAARAFAGQALSGLAVVLVAFTLLGE
                     IAMPWLMFILAPGFASDPEKFAAAVLFARIALPYLVFMSVVALYAGILNAFGRFAIAA
                     FTPTLLNVVLIVVLLAFVAAGGSNQSAAGIALAWGVAAAGLLQVLVVAIAAAKIGMRL
                     PWQRPQLSPEMRRLVALAVPGMLAGGMAQLAIVISTIIATLQDRVVSWLYYADRLFQL
                     PLGVIGVAIGVVLLPTLSHKLRSGDHAAVLESENRALEFALLLTLPAAIALYLAAEPI
                     MRVLFERGAFTAVDTKATASMLAALALGLPAYVLVKVLHPSFFAREDAKTPMLYAGIS
                     LAANVVLSLMLFLLIGATGIALATALSGWINVALLVAELTRRESFALDPIFRRRFLGI
                     LAACAVMGAVVFLLVKVLARGFAADSGLIVQGASLVVLVGAGLVTYLGAAHLFGAARF
                     RDLIKDARP"
     gene            complement(18064..>20280)
                     /locus_tag="VFY74_06375"
     CDS             complement(18064..>20280)
                     /locus_tag="VFY74_06375"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF003467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX5931360.1"
                     /translation="LFADFRRRFRNEVLGGNPRPFIEAKLAEQNARHARAGASRYVVE
                     PNIKDGKGGLRDLHTLHWLAKHLYFDKAEADFVEAGVFTAHEYRSFGRCESFLWSVRC
                     ELHFLTGRPEERLSFDLQQAMAERLGYRGHGGLSAVERFMRHYFLVAKEVGELTGIVC
                     SALEMKQLKSLPTLDTLLAPFSWRRRAKLRRTSDFRIENGRISAVDKNAFVKDPVNFI
                     RLFTFADEHQASFSPEVLRQIRSNFRLIDDKLRANPSANRLFMRLLTDARDPEGSLRK
                     MNEAGVLGRFIPDFGRVVSMMQFNMYHHFTVDEHLIRTMGFLSGIERGRFAAEHPVSS
                     EIIRTIDNRRALYVAAFVHDIAKGRDEDHSIVGARIARELGPRLGLNPSETETAAWLV
                     EHHLAMSHFAQSRDLNDPKTIRDFAALVQSRERLKLLLVLTVADIRAVGPGVWTGWKG
                     QLLRTLYFETEPLLGGGYTTLTRSERVARAQEALRARLTDWPEAELERFINRHAPSYW
                     LRMDLDVVAKHARLTRDAEARSMPIATHIATDAFRGVTEITLLAPNHPRLLAMVAGAC
                     TGAGANIVDAQISTTRDGMALDTISLEREFDQTEDEERRAERITSTIERLLNGNARLS
                     EVIATRRKPKPRLSAFTVEPQVVIDNTLSDALTVIEVNGLDRPGLLYDVTREISDLNL
                     DIASAHIATFGEKAVDVFYVTDLTGKKITSKSRESAIRERLLKALQARQEQAALPA"
BASE COUNT         3758 a         6408 c         6675 g         3439 t
ORIGIN      
        1 tttgccgcgg agaacggggg aaaagagggg gacccgtcgt aacccttcgg ccgttaaccc
       61 ttagggccgc aagttcaggc taaatccgct tgtgtccgct ccttggacca aatggaacag
      121 cggaatgcgg cggattgcag cctgctttcg ccttgtggcc gtgacctggc ctaaatggaa
      181 aagcggaatc gggcgggttg cagccctctt cagccttgtg gccgtgacct ggcttgcgct
      241 tggcttcagc cttgggtttt ggcctgagcg aatgcccttt tggcctgaca gactgcccgt
      301 ctacgacgtg gagctgggcg aaactgcggt cgagccccgg gtcgatcatc agggtatcgc
      361 cgctcgaagg gcggggggcc gggcgggggc gatgcgttcc gcatcctcta ccgctcgacc
      421 gggctgaacg gcgagccgat cgcggtctcc ggcgccatct tcatcccgcc ggggcccgcg
      481 cctgagcttg gccgcgacgt gatcgcctgg gcccatccga cgagcggcgt ggtcgaggcc
      541 tgcgcgccct tgctgatgcc cgatgttgcc ggcatgatct ggggtctgga ggacatgctc
      601 gggcaaggcc acgtcgtggt cgccaccgac tatcccggac tcggcgttgc gggccagatc
      661 cacccctatc tgatcggcgt gagcgagggg cgctccgtga tcgattcggt acgcgccgca
      721 cgcgatctgc cgcgctcggg cgcctccaat cgtttcgtgg tgtggggcca cagccaaggc
      781 ggccatgccg ccctctatgc cggcgagctt gccgcaagct atgcgcccga cttgaagctc
      841 ctgggcgtgg cgacggcggc gcctgccact tatctcgccg agttgttcga agccgacata
      901 tcgacaccga tcggcaagga gatgaccgcc atggccctct actcgtggac gaagctctac
      961 aacaagccgg cctcaaccct gatcgagtcc gccgccatgg cacccttcca ggaactggcg
     1021 catgaatgcg tcgagtcggt cctcgaactg gcggccctcg gtcgggcgga gaaaccgctc
     1081 gagtggaccc ggttcctgaa agtcgatccc accgagatcg catactggcg cgaaatcatg
     1141 gagcgcaaca caccggggca ggcgcccgcc ggcgcgccgg tctttatcgc ccaaggtacc
     1201 gtcgacacga ccgtgaggcc ggacgtcacc aagaaattcg ccgcggcgct gtgccggcaa
     1261 ggggagcggg tgcgcctcat ctctctcaat ggcgtgggcc acattttcgc ggccaagcac
     1321 agcgtcggcc aagcgctcgc ctggatgggc gcccgcttcc gcgggctgcc cgcgccgtcg
     1381 gactgcgaaa gctagcgcta gcctcgtcgc gtggcggcgc cgtcagtagc cgatcttgat
     1441 cagcgtgttt cccgtcccac gacgctgcac cagcgaatag agcgccgcgg cgtttgcggg
     1501 atgcagtctg atacagccat gcgaggccgg ccgcccgaga tgcttgatct cgtatgaccc
     1561 gtggatggcg taaccgcgca ggaagaagat cgaatgcggc atgggcgagc cgtcatagag
     1621 cgtggagcgg tggtaacgga cgagcgcggt cggtttgaag gtgccggtgg gcgtccggta
     1681 gccgcggcgc gccgtcgata tcggccagtt gtagcgcgcc ttgccatcga cgatgacggc
     1741 catgcgctgg ctcgacaagt tgattttggt gacgatctgc gcctcggcga gggttggaac
     1801 agcaaggatg aagccgaaga ggacggccag gaggacggtg cgccgcatgt cgtaactccc
     1861 tctaagccgg cctccgcggc ggctcttgtt gatatgaatc ccgggagcct gaggctccaa
     1921 agcaagacta ttcggcatct tttaacacca cgtccgtgaa catcggctga atgcgcttgg
     1981 caccgaaggt gcgcacccgc cgcggggcct gcgagacctt ccaattgccg ccatcatttg
     2041 tgctaggctg agcccgcttg agagccctgg ggggacggaa cgagggccga tgctaaggct
     2101 tttctttggc ctcctgctga tcatttatgg cggcgcggcc gcggctcagc agtcgctcgc
     2161 gccccaaacc gtcgccgcgc ccaagccggc gaagccttcg accaagcaaa ccgccaagaa
     2221 gaagccgccc aagcccaaat ctccgatcgc gcgcgaactg ttcggggctg cgagcgagcc
     2281 cgcccctctc gccgcccgct cgatcggcgg ctacgccaaa ggctgtctcg ccggcggcgt
     2341 gtcgttgccg atcaacgggc ccgactggca ggtgatgcgg ctctcgcgca accgcaattg
     2401 gggcaacccg cgccttgtgg actatctcga acgactggca agcgacgcgc atgcgctcga
     2461 cggctggccg ggcctcctcc tcggcgacat gtcgcagccg cgcggcgggc cgatgttgac
     2521 cggccatacc agccatcagg tcggactcga cgccgatatc tggctgaccc ccatgccgga
     2581 ccgcatcctc acgccggacg agcgcgagga catgactgcg gtctcgatgc tcaaggatcc
     2641 attcagcgtc gatcccgcga tcttcaccct cctccatgtg aagctcatca agcgcgccgc
     2701 atcgtacccg caagtcgcgc gcatcttcgt gcatcccgcc atcaagaagg cgctgtgcca
     2761 ggaggcgccg aaagccggca aagacttagc ttggctcggc aaggtcaggc cgtggtggaa
     2821 tcatcactac catttccacg tgcggctctc ctgcccgccg ggagcggaag gctgcgacaa
     2881 tcaaaagccg atcgccggcg acaatggctg cggtcaggag ttgaccaatt ggtacgccat
     2941 gttgaagaag gcggcgatcg cgagggggac gtcgccaaag cctggggcca agccctggac
     3001 cggcaagccc ccgctcacca tggcgcagtt gccgaaagag tgcggcgtgg tgctccgggc
     3061 cggcgggttc gaaccgccca tcccggcaga agacggcacg accgagaagc cgcccgccgc
     3121 gcttgccgcg ctcgcaacca aggatgcagg cccgcccatt ccgattctga ccgaggcgca
     3181 gctcaaggcc ctgaccggtg agaacgcggg cggcatgccg cttcccgacc gcaatcccct
     3241 ccgttgaggc ttcacgcgag gcgcgccatc gggcagtatc ccggccatgc aggagcgacg
     3301 tcaggagccg ctagcggggc agcgcaatgt ctatggcgag cccctcaagg cctgctcgga
     3361 gcggccgctc acggggttct tccgcaccgg ttgctgccat accgggcccg aggatctcgg
     3421 gctgcatacg gtctgcgtcg aggtgacggc ggagttcctc gccttctcca aggcgcgcgg
     3481 caacgatctc agcacgccgc aacccggctt cggtttcccg gggcttatgc ccggcgatcg
     3541 ctggtgcctg tgcgcggcgc gctggcgcga agccttcgag gccgggcgcg cgccgcgtgt
     3601 cgtgctcggc gcgacccacg aggcgacgct cgagatcgtc gatttcgaag atctgaagcg
     3661 ctacgccatc gatctcgcgt gaaggagttg cgcttatcgg gcctggacac cgttccgccg
     3721 gcgatggcag catgacgcga tgccgaaaaa aaagccgaaa aagaagccgc gcgcctcgac
     3781 caaacagagc gaacccgcaa cacaggtcag cgcgcgcaaa aagcgccaga tcttcgccga
     3841 gggccacaag cgcaagttcc tcgtggtcgt cgacgagacg cccgaatgcg agagcgcgct
     3901 tgcctttgcc gcaagccgcg cgcagcggac cggcggccag ctcgcgctgc tctacgtcat
     3961 cgagcccgac gtcgatttcc agtggctcgc ggtggaagat gtcgcgcgcg aggagggaca
     4021 cagcaaagcg aaggccgtgt tccgcctgtt cgggcgcaag ctgaaagcca tgggtttcga
     4081 ggcgctcgtt ccggaggaga tcgtgcgcga gggcatgaaa tcggaggaaa tcacgcgggc
     4141 gatcgaggag gacgaggata tcggggtcct cgtgctcgga gcctcgaaag acccttcggg
     4201 acccgggccc ctggtcacgg accttgccgg cagcgggctc ggagcgacct ttccggtccc
     4261 gatcacggtc gtgccggggc atctttctac tgaagaaatc ctcgctttgg cttgatatgg
     4321 ggcttttctg ccccgacatt atcagccaaa atttgagcgg ttttcagtgc tagcggaatg
     4381 ggacggtttc aaggggcgcc attcgaggct tgagggagcc aaccatgaga atagcggcat
     4441 tgggcctgct cgcggcggcg tgcttcagcg ttcccgcatt tgcggccgag cctaacggac
     4501 cggccggtct catcacgccg gccgaaggtt tgcgcattac cgttcaaggc ctcgcctccg
     4561 ccaagtccaa ctcgccgcaa agggacgcgc tggtccaata ttacggcgcg cccgaccgga
     4621 acctcctttg ggtcgatgac aacgggctca ccgagcgcgc caagctcgtc atggccgaga
     4681 tcggaaaggc cgatgattac ggcttgcgag cctccgatta tccgctgccc gatgccgcct
     4741 catttaaccc ctccgatccc ggggcgaggg actggctcgc ccaatcggag atgaaggtca
     4801 gctacgcggt gctcgactat gccaaggacg cccgcggcgg ccgcatcgaa ccggtgcgcg
     4861 tcagcaagaa tctcgatccc aaccttgccc tccccaatcc ctccgaggtg cttgaggtga
     4921 tcgccatccg ttccgatccg gccgcctatt tgcggagctt tcagcctgcc cagccgcaat
     4981 tcgaggctct caggcagaag ttgatcgagc tcagaggcgg caaggtcgaa gaggtcaagc
     5041 ccaacgccgt cgtcattccc gccggtccgg tgctcaagtt cggcatggag cacgagcagg
     5101 tcgcgctctt gcgcaagcgg ctcgacgttc ccgccggctc aaacgagaac aagttcgacg
     5161 aggccgtgct cgacgcggtg cggcagttcc aatccggcaa cggcacgcgc cccgacggcg
     5221 ccgtgggcgc gggcacgcgg cgtctgctca atcatggcca acagccgcgg gccgtgggca
     5281 gcgaggcgcg gatcaggcag gttctcgtga atatggagcg ttggcgctgg ctgcccaacg
     5341 atctcgggcc gttctacgtg aacgtcaaca tcccggagtt cacgctacgc gtggccgaag
     5401 acggcaaacc catccacacg gcgcgcgtgg tcgtcggcaa acccaggacg cagacgcccg
     5461 tgatttccga tgagttggag gtgatcgtct ttaatcccta ttggaatgtc ccgaactcca
     5521 tcaagaacga agagatcgcc ccttacatgg gagcgggcgg cggctttttc ggcggcggct
     5581 gggacacttc cgttctcaag cgtcacggtt tgcgcatcaa gtacggcaac cgcgatatcg
     5641 accccgattc gatcgattgg agccgcaatg acatccgcaa tttcgatctc gttcaaccgc
     5701 ccggccccag caacgtgctc ggccgcgtga agttcttgtt ccccaattcg cacgacgtct
     5761 acatgcacga cacgacgcag aagttcctgt tcaacaacgc cgtgcgcgcc gagagtcacg
     5821 gctgcatgcg cgtgcagaat cccaatctgc tggcgcaggc gatcttgaag cgcgacaagg
     5881 gttggagcga ggcgcgagtc gaatccacgt tcgatgccgg cgacgaccat caggtcgccc
     5941 tcgaccagaa gattcccgtc tacgtcacct atttcacctt gagggtgaac gaggacggca
     6001 cgttcacctc gttcaacgat ctctacggtc acgactcgag aatgacggcg gcgctcgagg
     6061 ggcggggcta ccagagcgat ccggtgtacg aggaggaaca ggtgttcgcc ggagagtggc
     6121 ggcaaaacca gcggcgggtc ggagggagag ggtccttcgg cagcgatttt acccggtctc
     6181 tgtttggatt ttagcggcct ttcgcgctgg cacggcgcgg cggcgagcag ggcgtgcgcc
     6241 ttgactttcg cgcgcgtggg accaagtata agctagaatc gttctaagat cagcggcgcc
     6301 ttgccgccgg agagccatgt tcattcagac cgaagcgacc cccaacccgg cgaccctgaa
     6361 gttcatcccg ggccgccccg tgctcgccca gggcacgttg gagttcctcg atcccgagac
     6421 gagcaaagcc tcgccgctgg cggcacggct gttcgagatc gagggcgtca agggggtgtt
     6481 cttcggcacc gacttcatca gcgtgaccaa gggcgagggc gagtggcagc acctgaagcc
     6541 tgccattctc ggcgtcatta tggagcacta tctctcgggc gccgccgtga ccgacgcgcc
     6601 ggaggaggcc gaagcgggcg aaccgaacga gttcttcgct cgggaagacg aagagacggt
     6661 caagaccatc aaggagctgc ttgagacccg cgtccgcccg gccgtggccc aggacggcgg
     6721 cgacatcacc ttccagggct tccgcgacgg cgtggtctat ctccacatgc gcggcgcctg
     6781 ctcgggttgc ccgagctcca ccgcgacgtt gcgtcacggc atcgagaatt tgctcaggca
     6841 cttcgttccc gaggtgcagg aggtccgtcc ggtttgacgg accagctccc cgattcctgt
     6901 ctcgaccagc tgttcagaac cgcgcgcacg cataacgcgt ggcgcgagcg ggacgtcccc
     6961 gacgcgctgc tgcacgagat cgtcgatctc gccaagctcg ggccgaccag cgccaattgc
     7021 agcccggcgc ggctcctctt cgtgaagtcg cgcgccgcca aggagcggct ccgcccgcat
     7081 ctctccgaag gcaatcgcga caagacgatg aaggcgccgg tctgcgtcat catcggctac
     7141 gatctcgact tctatatcca tctgcccaag ctctttcccc ataccgatgc caagagctgg
     7201 ttcgagggca agaaggagaa gatcttcgac aacgcctttc gcaacggcac gctgcaaggc
     7261 gcctatctca tcatggcggc gcgcgcgctc gggctcgatt gcgggccgat gtcgggcttc
     7321 gacaacaaag gcgtcgataa ggagttcttc gccgggacca acatcaaatc gaattttctc
     7381 tgcaatctcg gctacggcga tgcaagcgtc ctgttcccgc gctcgccgcg cttcgaattc
     7441 gacgagatgg cgcaaatcct ctaagcaagc gccatgaata tcctcgcgct cgagaccagc
     7501 atgggcgcct gctcggccgc gctgtttagg cgtgacggcg gcgcgtgcac cgtgttcgcg
     7561 cggactgaga agatggcgcg aggccacgcc gaagcgctga tgcccatggt ggcgcaaatc
     7621 atgagcgaga gcggtacggc gttcgccgcg ctcgatcgaa tcgtggcgac gatcggcccg
     7681 ggcagcttta ccggtgtgcg gatcgcaatc tcggcggcgc gcggtcttgc gcttgtgacg
     7741 ggagccgcgc tttttggcat cgacagtctg acggtgatgg cgaaagaggc gctcaccttg
     7801 ggcgttgcgg gggccgcgcc atttgccgtc gccgtcgatg cgaggcgcgg catgctctat
     7861 ctcgggctct atgacggtgc cgcgcgaaga cgcgagggcc cgctcctgat cgcgccggat
     7921 gatgccgcga atttgctccc gagcggactt cgcacggccg tcggcagcgg ggccgcgcgg
     7981 ctcgccgagg cggccgtcag ccacggcgtc cacatcgagg cgaagctgct cgatcttgag
     8041 ccgagcgcct catcccttgc cgcgctcgcc gaggagagcg gcgagacggc gctcacgctc
     8101 cggccgcttt acttgcggcc ccccgacgcg aggccccaaa cgcaggcgag tgtggcgcga
     8161 tgctgacgac cgtcgagctc aaagtgcgcc aggcgggtcc cctcgacgcg gacgtgctgg
     8221 ccgctctcca ccgcgattgc ttcgcgcggg cctgggacga ggccgcgatg ataaagttcg
     8281 ccgcgtcgcc cgagacgcta tgcctgatcg gcgcggtcgc agccggcgct tcaagcgtcg
     8341 ccggaggttt gctgatcgcg cgaaaggccg gagatgaggc cgacattctg accttcggcg
     8401 tcgtgccgtc gtaccgcaac aggggtcttg ggcgggcgct ccttggcacc gcgactgagc
     8461 gcttgcgcgc aagcggcacg aagcggttgt ttctcgaagt ggaggaaagc aacgacgcgg
     8521 cgcttcggct ctatcgctct ttcggcggtg agccggtcgg ccggcggcaa ggctactatg
     8581 acgacggcgc cgacgccgtg atcttcagcc ttgccctttg acgccccctt gccgatgatg
     8641 tcgcgacgcg ggcgagaggc ggcaaggaag ccacatgcgc aagagggcca tgagggaaag
     8701 caggctcgaa cggctctgcg ccgaaaaggg catgcggatg acgggtcagc gacgaaccat
     8761 tgccaaggtg ctgtcggagg ccgacgatca tcccgacgtc gaggaggttt accgccgtgc
     8821 aagcgcccgg gacccgcata tctcgctctc gacggtctat cgcacggtgc gcctgttcga
     8881 gggcgccggc atcctcgagc ggcatgattt cggcgacggc cgcgcccgtt atgagcccgc
     8941 gggccccggc catcacgatc acctgatcaa cgtcaagacc ggcgaggtca tcgagttcag
     9001 caacgagcag atcgaacggc tgcaggagaa cgtggcgcgc gaactcggct tccgccttat
     9061 cggccaccgt ctcgaacttt tcggcatgcc gctcaagaac ggcaaatcgt agaccggcgc
     9121 gtgcgcgagg cgatcttagc ggcaacgtca tgagaagctt gcgtgggttc ctcgttcttg
     9181 gcgtcttctt tgccttcaca ctgccgctga tgccgcttca gctcctgttt ctcagcatcg
     9241 gctcgcgcca cgcccgcact tttccgcatt ggtaccatcg gcaggtgtgc aagatcgtcg
     9301 gcatccgcct caatatcgaa ggcaagatcg ccgaggggca cggagtgctg ctgatctcga
     9361 accacgtctc gtggatcgat atcagcgtgc tctctgcggt ggcgccggtc tcgttcgtcg
     9421 ccaagcaaga ggttgcctcc tggccgttcg tgagctggct cgcgaaattg caacgctcgg
     9481 tgttcatcga ccgcactcgg cggagcgagg tcggcgagaa agcgaatgag attctgaacc
     9541 ggctcgccgc aggcgaccac atcgtgctgt tcgcggaagg cacttcaagc gacggcaata
     9601 gcgtgatccc gttcaagacc tcgctgttcg cggcggccaa gccgcccggg ggcgcgccca
     9661 tgggcgccaa ggtttcggcg cagacgctcg cgctcaccta caacaaagtc tacggcttgc
     9721 cgctctgccg gcgcgaccgt cacctcatcg cctggtatgg cgaaatggac ctcgccacgc
     9781 atgcgtggag gctgttaggg cttggtccta tcgacgcgca tatccgcatc gggccgccga
     9841 tgcccctcga tcaatttccc gaccgcaagg cgctcgcgcg ctacgcagag gacaaggtgc
     9901 gcaatgacgt ggccgaactc ctgcgcggac gcaaagacgc ggaggcggcg ccatcgcact
     9961 gacggtgcga gcgaagactg gagcgccgca gccgggacgg cgacagcgct ccagactcaa
    10021 gaaagatcac atcgcccgtc ttagcctgat cgtgtcgagc acggtcgcgg gcgcgaagtt
    10081 cccgttcggc aattgccgaa tgagaccggc cttcaccaca ttgatcagag tggcatgcac
    10141 accccgatcg gcggtctcgg cgtcgacgaa atagccctcc tgcaaagcga accgccggag
    10201 gtcgtcccgc gacatggcgc ccgcttcgtt gagcttgcgg atgaggaagt cggccaacgg
    10261 ctgctgcggt tgacgcggcg ccggcggctg tggctgtggc tgtggctgtg gctgcacgtg
    10321 ttgatgcaga ggctgaggct gaggctgagg ctggacgtgt tgatgcggct cgtgcaccgc
    10381 ttggctcgcg gcctcgtgca ccagcggcct ttgtcccgcc gctgacgcct ccagtcgccg
    10441 gcgttcgagc tccatcagcc ggttcacagc ctcgagttcg gcctttaatt cctcgagcct
    10501 cgtttcgtgc tcttgcttga gctgctcgta tagtcggtcg aattgggatt cctgggcgtt
    10561 gatctctcct tccaagagag tggcccggtc ttgcaggtcc ccgcgaatgt cacgcattgc
    10621 tgcccctcgt tcatggtgtt ggctccaacg acccgacggg gctggcaggt tcacgcaaga
    10681 ccaaacgaac catgcctggc cgccgcgccg ggcaagtctt gcaaggcccc gggattgcct
    10741 cggcaggggc cctactgcct catcttaacc acaagggaag gccgctgtct cggctgagct
    10801 tcccggattg acatacacca agctttcagc gtgtggacag acccatgcgc caagccttgg
    10861 cgaacgcggc ttcggccgtg gcgaagacga gaccaagctt tcaagtgaat tcagacgtcg
    10921 ccttgcggca aatgccgtgc cttattcttg gcaaccgtcg atcgaagcgg cggcaattca
    10981 gcgcctatgt cttgccgtcg ctcttttcgg ctttgccatc tcgggcagaa acgccgcaag
    11041 caacccgatc gccggcagaa aagcgcacac atggtagacc aaggtgatgc tggtcgcgtc
    11101 cgcaagccag ccaagaaaag cggcaccaat gccggcgacg ccgaaagcaa ggccaaagaa
    11161 caagcctgcc accatgccga cccgacccgg gatcagcgtc tgggcataga cgatgatcgc
    11221 agggtgcgcc gaggcgagga tcagcccgat gatcacgctc aagatcgcgg tgcccagcaa
    11281 acccacatag ggaagcgcaa gcgtgaaggg cagcacgccg agtatcgacc accagatcac
    11341 gcgccttgtg ccgatgcgat cgccgatcgg accgccgatc acggtgcctg cggcgacggc
    11401 gaacagaaac aggaacaggt agagctgcgc gttctgcacc gacagcccga aggtgtggat
    11461 cagatagaag gtgtagaagc tcgtgatgct gacgaggtag aagtccttgg agaaggtgag
    11521 cgccatcaag atggcgaccg acttccgcac cttgccgtgg gtcagctcgt ggatcgcttc
    11581 gcgctcgctc ggatgcgcag tgctgttgaa cttcagcgtc ctgccatacc aggagccgat
    11641 cgcggcaagc atgaccatgc cgatcagcgc cacgatcgag aaccaggcga ggctttgctg
    11701 cccgaacggc agcacgatga aggccgcgag cagcggaccg agcgcggtgc cgaaattgcc
    11761 gccgacctga aacacggatt gcgcaagccc atgctgtccg cccgaggcaa ggcgcgccac
    11821 gcgcgaggat tcggggtgaa acaccgagga gcccatgccg atcaggccta cggcgacgag
    11881 cacgaggccg taggtcgagg caaaggcggc gatgaggagg ccgagaagcg aacagcccat
    11941 gccggcaacg agagaatagg gcgtggggta acggtcggtg acaaaaccga ccaggggctg
    12001 aagcacggac gccgtgaacg tgaaggtgaa ggtgatgagc ccgatctgac ggaagtcgag
    12061 cccgaggctg tccttgatca tgggatagag cgccacgatc agcgactgga tcacgtcgtt
    12121 caagaggtgg cagatgctga tggccgcaag caccgcaatc gcggtctgct gcgcggccga
    12181 tgcgcccgtg gtctcgccgg tcttgatcgc catgcttgag cctgaaatgc ttcttgtcgg
    12241 gcggcgcaga attggggcga acccagtctc aaaagcaagg gctgatttcc gctcggtgca
    12301 gcggtcggcg caaccgttac gccgcggtct ggcgcccgac cggtaggctc aagccaggat
    12361 agaaccgggc catcatccgg tccgcgtagc ctctgagatt gtcgtgccgc tccgcggcga
    12421 ggcgaatcgg cgtctcgaat accggacaca gcacgccggc gacaaacgca aaagacgtgg
    12481 cgtcgacgcc cgtcggttcc gctcccatca tgtaaggctt ggtccccaag aaatccgccg
    12541 tcgcgtcgat cgatcgcgtc gcaagcgccg tgatctcctc ggaggaatgg cgccccatgc
    12601 cttgtccatg cagcctttgg cgaacttgcc tccggatcat ggccaccacg agaggccgga
    12661 tcggtgccgg tacggcgcgg aagaaattgc gcggtccctt cgcgaagttc gcgtcatcca
    12721 tccagcggtc atgcacaacc gcccaataga gattgtcttc gagcatcttc tcgaacgccc
    12781 aggcaacggc gcgctgctct gcgttaaggc cgcggtcgaa atcgatgttg tacttcttct
    12841 cgagatgcca gcggatgaac gtcgagtcgc cgatgggctc gccgtcatcg accacgtagg
    12901 gcagcttgcc cttgggcgcc ttgctcaagc cacccgtctg aacggcgtag ggcacgcccg
    12961 ccatcttgag gagaatctcg gccttcatca cgaaggggct cggatcggga aggccgaagg
    13021 cgggtccaaa gccgtaaagc gttatcatct gtcatccctc atggcatgga cgcggggctc
    13081 ggctgtcatt ctatcctacc gcacggacgc gcaggcacgc cctttccatg cgcgcgcgat
    13141 gagggcggcg caggccgtta acaacacatc aaagaccgcc gaaatccacc ggaacgaacg
    13201 ctgcgggcaa acgtttcgtt catgaggggc taaccgccaa gcccagttcc gggctgggcg
    13261 ccgttgaatt caaggagacg cctatgaacc gccttttgac gatacctgcc agccttttga
    13321 ttttaggttt ggcaccggca ttggcagtcg aagacgccgc caaaccggat ccgtccgcga
    13381 cgggggcgga agcgggccag cttcagactc agtcgggagt gagcgaggac gcggccaaga
    13441 aagccaccga gccggccgag ggttcggccg atacctctgg tggcgcaatg gagcgtagct
    13501 ctgcgcctcc gtcatccgac gccgcggcta ccgcgaagga ggacgcctcg aaggaagccc
    13561 agtcgtccga tacgtcgggc ggcgccaagg agcaaagctc ggctccggag ggctcgtctg
    13621 cggccgacaa gtcgaagccg aaccccacca ttgggtccga gacgcccaag gaggatgaca
    13681 cgtccaagga gtaatcaatt acttcttgga aagtgacagc aaggggccgc gttgaaagac
    13741 gcggcccctt cgcttttgcg cacgcctcac cgagccgcag ccgggagctc accatgcaat
    13801 tttttcaagc cgagcgacga tggcggagag ggtgggattc gaacccacga gacggttgcc
    13861 cgcctacacg ctttccaagc gtgcgccttc gaccactcgg ccacctctcc tctcggcaaa
    13921 gcgcctctcg ccgggcgccg cactatagcg gagcaccccc gaggttcaag caagcgggag
    13981 gtttgctcgc gcgccgctca cgcaatgctg acccggcgcc catttgaaca tgagccggcg
    14041 atggcttatg taggggtcat cgcttccatc cggtagcata tcggcacatg cgaacattgt
    14101 tgcgcattgt tggcgtctgg ctgctcttgc tcgccatggt cgccgccgtc atcgacgcca
    14161 ccaagagcct ggcggggggc ggcgcgtggg tggtgacgcc gatgggccag cagtggcagg
    14221 cgctgagccc ggagacgctt cgcgacgcca aggccgcgat cgagagcaag gtcagcccat
    14281 ttttgtggga cccgatcatg gcgacgatcc tcgacgcgcc gacctgggtc gtgttcggca
    14341 ttctcggggt cctgctttat tggctcgggc agaagcgcaa accggtcgaa gtgttcatca
    14401 attaacgggg gaacggatca gcagctgcgc caaagggagt ccacgatgtt cacgctgaag
    14461 cgcaagcctc tgtcgatgcc ctcgcccgcc gaggcgttgc ccgggcgcga aaagcccatt
    14521 gccaccgcaa agacccattt cgtcaacggc gccgcgctga aaggtcccta cccgcaaggc
    14581 ttcgagaccg cgctgttcgg gctcggctgc ttctggggcg ccgagcggac gttctggcag
    14641 ctccccggcg ttcacgtcac tgccgtcggc tatgccggcg ggctgacgcc gaacccgacc
    14701 tacgaggagg tctgttcggg gcgcaccggt cacaacgagg cggtgctcgt cgtctacgac
    14761 cccaagcgcg tgagctacga tgagctcctc aaggtgttct gggagagcca tgatccgacg
    14821 caaggcatgc ggcagggaaa cgacgtgggc acgcaatacc gctccggcat ctactattat
    14881 tcggagccac agcgcagcgc ggctgaggcg tcgaagacgg cctacgaggc ggcgctcaag
    14941 gcacgcgggc tcgggcccat cacgaccgag atcgtgccgg cgcccgagtt ctatttcgcc
    15001 gaggactatc accagcaata tctcgccaag aacccgcacg gctattgcgg gctcggcggc
    15061 acgggcgtct cctgcccgat cggcaccggc gtcggcgccg cctaaccatt gtccgcctcg
    15121 aggaggccct ttcggcgctt ccttgctcgt catgcccgga cgtctcagcc agttgaagcg
    15181 ccaatggaat ggattgccgc gtcaagcccg gcaatgaccg ggaggatgga gcgtggcgag
    15241 cggtaaccac gtcattccgg cgaaagccgg aatcccagaa gcggcgtgca ctgcgttcgt
    15301 taattctgga taccggcttc cgccggcatg acgaccatca ccccacccgg ctcggccttt
    15361 ggccgagcca ccctcccctt cagggaggga tagttgcgtc agctgcggat gaagccgacg
    15421 atgtccttgg tcttggcgag gatcggatcg gcgatggcgc gagcgcggcg cgcgccgtcg
    15481 gcgaggatgc ggtcgatctc gaccggatcg gacatgaggc ggcgcatctc cgcaccgatc
    15541 ggtgtcaatc gcgccaccgc aagcgcggcc agagcctcct tgaagcgcga gaagggcgcg
    15601 ttgccgaaat cgcgcaacac cgcttccttg gtcgtgtcgg cgaggcccgc atagatgccg
    15661 acgagattgt cggcctcggg cctctccgca agccccttca cctccgacgg cagcggctcg
    15721 ggatcggtct tggccttttt gatcttcttc gcgatcgcat cttcatcgtc ggtcagattg
    15781 atgcgcgtca tgtcggactc gtcggacttc gacatcttgc gcgtgccgtc gcggaggctc
    15841 atcacgcggg tggcaggacc ggtgatgaga ggctcgggca gcgggaagaa gccgccggga
    15901 tagccttcgc gcgcgatcgt cgcggcgaag tcgttgttga atttctgggc gacgtcgcgc
    15961 gcaagctcca gatgctgctt ctggtcgtcg ccgaccggga cgtgcgtcgc gtggtagagc
    16021 agaatgtcgg ccgccatcag gttcggatag acatagagcc ccgaagaggc gttctcgcgg
    16081 tccttgcccg ccttctcctt gaactgcgtc atgcggttca gccagccgag ccgcgtcacg
    16141 caattgaaga tccaggcaag ctcggcgtgg cctgagacct ggctctggtt gaacacgatg
    16201 tgtcgggcgg gatcgatgcc cgaggcgatg aaggcggccg tcacctcgcg ggtgttcttc
    16261 ggcaactcct cggggtcctg gaatacggtg atggcgtgga gatcgaccac gcaataaatg
    16321 cagtcatgcg cggcctgaag cgccacgaat ttggtgatgg cgccgagata gttgccgaga
    16381 tggagattgc cggtcggctg gatgccggag aagacgcggg tcttggtctt gaatctttgc
    16441 atgtcttggc cctaacgctg gcgggttatg gcttggcaag cggcccggcg caagcatgcc
    16501 agccgccggc tcaaggacgg gcgtccttga tgagatcgcg aaaccgcgcc gcgccgaaga
    16561 gatgggccgc cccgagatag gttacgagtc ccgcgccgac gaggacgacg agcgaggctc
    16621 cctgcacgat cagcccgctg tcggcggcaa agccccgggc caagaccttg accagcaaaa
    16681 agacaaccgc tcccatcacg gcgcaagccg cgaggatgcc gagaaaacgc cgccgaaaga
    16741 tcgggtcgag ggcgaagctt tcgcggcggg tgagctcggc cacgagcagg gcgacgttga
    16801 tccaaccgct aagcgcggtc gcgagcgcga ttcccgtggc gccgatgaga aggaacagca
    16861 tgaggctcaa gacaacattc gccgcgaggc tgatgccggc atagagcatc ggcgtcttgg
    16921 cgtcctcgcg ggcgaagaag ctcggatgca gcaccttgac taggacataa gccgggaggc
    16981 cgagggcgag cgctgcgagc atggacgcag tggccttggt atcgaccgcg gtgaaggcgc
    17041 cccgctcgaa cagcacgcgc atgatcggct ccgccgcaag gtagagcgca atggcggcgg
    17101 gcagggtgag gaggagcgcg aattcgagcg cccgattttc gctctcgagc acggcggcat
    17161 ggtcgcccga gcggagcttg tggctgaggg tgggcaagag gaccacgccg attgcaaccc
    17221 cgatcacgcc gagcggcagc tggaacagtc ggtcggcata atagagccaa gacacgaccc
    17281 gatcctgaag ggtggcgatg atcgtcgaga tcacgatggc gagctgggcc atgccgccgg
    17341 cgagcatgcc gggcaccgcg agcgctacga ggcgccgcat ctccggcgaa agttgcggcc
    17401 gctgccatgg gaggcgcatt ccgatctttg cggcggcgat cgcgacgacc agaacctgca
    17461 acaggcccgc ggcggcaacg ccccaggcga gcgcaatgcc ggccgcgctc tgatttgacc
    17521 cgccggccgc gacaaaggcg agcagcacaa cgatgagcac gacattgagg agcgtggggg
    17581 tgaaggcggc gatggcgaag cggccgaacg cgttgaggat gccggcatag agcgccacga
    17641 ccgacatgaa cacgagataa gggagcgcga tgcgcgcgaa gagaacggca gctgcgaatt
    17701 tctccggatc gctggcaaaa cccggcgcga gaatgaacat cagccacggc atggcgatct
    17761 cgccaaggag cgtgaaggca acgagaacga cggcgaggcc ggagagcgcc tgtccggcga
    17821 aggcacgtgc cgcttccgga tcggcgtgga tgcgcttgga gaagagcggg atgaaggcgg
    17881 aatcgaaggc gccctcggca aacagtcggc gaaacatatt ggggatgcgg aacgccacga
    17941 aaaacgcgtc ggccacggtg gatgcgccga gaaccgcggc aatcagaatg tcgcggacga
    18001 agccgagaat gcggctcgcg atggtgatag tgccgacggt ggcgaagccg cggacgagct
    18061 gcatcaggca ggaagcgccg cctgctcttg acgcgcttgc agcgccttca ggagccgctc
    18121 gcggatcgcg ctctcgcggc tcttgctcgt gatcttcttg ccggtgagat cggtcacgta
    18181 gaacacgtcg accgccttct cgccgaacgt ggcgatgtgg gccgaggcaa tgtcgagatt
    18241 gagatcggaa atctcccggg tgacgtcgta gagaaggccc ggacggtcga gcccgttgac
    18301 ctcgatcacg gtcagcgcat ccgacaacgt gttgtcgatg acgacttgcg gctcgacggt
    18361 gaaggccgag agccgcggct tgggcttgcg cctggtggcg atcacttccg acaaccgcgc
    18421 attcccattg agcagacgct cgatcgtcga cgtgatcctc tcggcgcgcc gctcttcatc
    18481 ctcggtctgg tcgaactcgc gctcgagcga gatggtgtcg agcgccatcc cgtcccgcgt
    18541 cgtggagatc tgcgcatcga cgatgttggc gccggccccg gtgcaggcgc cggcgaccat
    18601 ggcaagtagt cgcgggtggt tcggtgcgag cagcgtgatc tcggtgacgc cgcggaaagc
    18661 atcggtcgcg atatgcgtgg cgatgggcat cgatcgcgcc tccgcgtcgc gagtcagcct
    18721 cgcgtgcttg gccaccacat cgagatccat ccggagccaa taggacggag cgtgccgatt
    18781 gatgaagcgc tcgagctccg cttccggcca gtccgttagc ctcgcgcgca aggcctcttg
    18841 cgcgcgcgca acgcgctcgc tgcgcgtcaa ggtcgtatat ccgccgccga gcagaggctc
    18901 ggtctcgaaa tagagcgtgc gcaggagctg gcccttccat ccggtccata cgcccggccc
    18961 gaccgcgcgg atatcggcga cggtcagcac gagcaggagc ttcaatctct cgcggctctg
    19021 caccaacgcg gcaaaatcgc gaatggtctt gggatcgttg aggtcgcggc tctgggcgaa
    19081 gtggctcatg gcgaggtgat gctcgaccag ccaggcggcg gtctcggtct cggacgggtt
    19141 gagaccaagg cgcgggccga gctcgcgggc gatgcgcgcg cctacgatgg agtgatcctc
    19201 gtcccgcccc ttggcgatgt cgtgaacgaa ggcggccacg tagagcgcac ggcgattgtc
    19261 gatggtgcgg atgatctcgc tcgataccgg atgctcggcg gcgaatcgcc cgcgctcgat
    19321 gcccgataga aatcccatgg tgcggatcag atgctcgtcc acggtgaagt ggtggtacat
    19381 gttgaactgc atcatcgaga cgacgcgccc gaagtccggg atgaagcgtc cgagcacgcc
    19441 cgcctcgttc atcttgcgca aggagccctc gggatcgcgc gcgtcggtga ggagacgcat
    19501 gaacaggcga ttggccgagg ggttggcgcg aagcttgtcg tcgatcaggc gaaagttcga
    19561 gcgaatttga cgcaacactt ccggcgaaaa cgacgcctga tgctcgtcgg cgaaggtgaa
    19621 caggcggatg aaattcaccg gatccttgac gaaggcgttc ttgtcgacgg cagaaatgcg
    19681 gccgttctcg atgcggaaat ccgacgttcg cctgagcttg gcgcggcggc gccagctgaa
    19741 gggggcgagc agcgtgtcga gggtcggcag cgatttcagc tgcttcatct cgagcgccga
    19801 gcagacgatg ccggtcaact cgccgacctc cttcgccacc aggaaatagt gacgcatgaa
    19861 ccgctcgacc gcgctcaagc cgccatgccc gcgatagccg agccgttcgg ccatggcttg
    19921 ctgcaggtcg aaggagagac gctcctcggg gcgcccggtg agaaagtgca gctcgcagcg
    19981 cacgctccag agaaagctct cgcagcggcc gaagctccga tactcatggg cggtaaagac
    20041 gccggcctcg acgaagtccg cttccgcctt gtcgaaataa aggtgcttgg ccagccaatg
    20101 cagcgtgtgc agatcgcgca accccccctt gccgtccttg atgttgggct cgacgacata
    20161 gcgcgaggct ccggcgcggg catggcgcgc gttctgctcg gcgagcttcg cctcgatgaa
    20221 gggccgggga ttgccgccaa gcacctcgtt gcggaaacgg cggcgaaaat cggcgaaaag
//
LOCUS       DASUQP010000144        12653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_890344,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000144 DASUQP010000000
VERSION     DASUQP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12653
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890344"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFY74_06380"
     CDS             <1..531
                     /locus_tag="VFY74_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5931361.1"
                     /translation="EMLTFRSLLDQTSDRRERKAMLAQKVEDMINTVVRQIAFYSFER
                     KVHTERRDGELTSDRLGEVWLDVQRESLGPAIDLKTGYETFWCYIPHFIHAPFYVYAY
                     AFGDCLVNSLYAVYEKADKGFAERYLDMLAAGGTKHHKELLAPFGLDASDPNFWSMGL
                     KVIEGLIDRLETIDRG"
     gene            675..899
                     /locus_tag="VFY74_06385"
     CDS             675..899
                     /locus_tag="VFY74_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX5931362.1"
                     /translation="MKIEIKRIGNSEGFILPRELMQRLGLKRGQTLHVTELAGGGFQA
                     LPYDPDFEKTMEIVDEIIDEYRDTLSALAK"
     gene            896..1294
                     /locus_tag="VFY74_06390"
     CDS             896..1294
                     /locus_tag="VFY74_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX5931363.1"
                     /translation="MTEPKWFTYEQAIAAHSAQLIRYGGASGLRDEGALRSALDRPVN
                     KWHYERAGLPELAAAYAFGLAKNHPFVDGNKRISFIAMVTFLRKNGVAFRPEQAQSTA
                     IILAVAAGEVSEESLTRWIRDNWPKGSSSP"
     gene            complement(1427..1873)
                     /locus_tag="VFY74_06395"
     CDS             complement(1427..1873)
                     /locus_tag="VFY74_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEX5931364.1"
                     /translation="MTPRSVLAFFLAITWIWPDLAAAEGRAEVWKSPSCECCGAWVEH
                     LQTNGFDVKVNAAIPSMLDRIKREAGIGEQLAACHTAKIGAYVIEGHVPAADIERLLA
                     EQPDALGLTVPGMPVGSPGMEQDGKTEPYDVLLIKKDGTTEIFARH"
     gene            2003..3382
                     /locus_tag="VFY74_06400"
     CDS             2003..3382
                     /locus_tag="VFY74_06400"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX5931365.1"
                     /translation="MNDRQSDKEKNRFSARAIRYGRVGANVGTVAARIAARRLLGLDP
                     ERDRDAAALAAALGGLKGPIMKVAQLLATIPEALPAEYAAELGQLQSQAPPMGRSFVR
                     RRMAAELGPDWETKFASFEQEAAASASLGPVHRAVADGRALACKLQYPDMQSAEEADL
                     VQLAVLFSIHARMSPAIDTSEMLKEISARLREELDYDLEARHTRLYGVIFERDPLIRV
                     PEVLAELSTKRLLTMTWLEGRRLLDYTSRPLEERNQIARAMFRAWWYPFSHYGVIHGD
                     PHLGNYTIFEDGEGRTAGINLLDYGCMRTFAPKFIQGVVDLYQGLLRKDRALIVHAYE
                     TWGFTGLSNELIDILNIWANFIYGPMLEDRVRTIAEKVSPGSYGRQEAFKVARGLRER
                     GPVKVPREFVFMDRAAIGLGGVFLHLNAELNYHRLFNDTIAGSDLADVAGRQRAAFGA
                     AGVPLPEES"
     gene            3412..4470
                     /locus_tag="VFY74_06405"
     CDS             3412..4470
                     /locus_tag="VFY74_06405"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX5931366.1"
                     /translation="MRKAKAYSAASATSSLASDTIPRRDPTERDVQIEILFCGICHSD
                     LHQVRNEWSGVMPTVYPCVPGHEIVGRVTGVGAAVSKYKPGDLVAVGCMVDSDRTCPH
                     CREGLEQFCPSMILTYNSPDKHLGGVTYGGYSESIVVDEHFVLRVPSNLDLAGAAPLL
                     CAGITTYSPMRHWGVSAGKKVGVVGLGGLGHMGVKFAHAFGAHVVVFTTSPGKKDAAL
                     GLGADEVVVSRNADEMKKHAGSFDFILDTVSADHDINAYLNLLRRDGNMVLVGAPEEF
                     LGVLAFSLLVGRRSLSGSIIGGIAETQEMLDFCGEHNITADVEVIPIQKVNEAYERLL
                     KSDVKYRFSIDMASLKSG"
     gene            4741..4884
                     /locus_tag="VFY74_06410"
     CDS             4741..4884
                     /locus_tag="VFY74_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX5931367.1"
                     /translation="MRIDPKEGSPAMDYAEHMKTYKLFCKLTAFAILGAVVVMVLLAL
                     FVA"
     gene            4997..6217
                     /locus_tag="VFY74_06415"
     CDS             4997..6217
                     /locus_tag="VFY74_06415"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX5931368.1"
                     /translation="MIAIGVPAESEDEPRIGLTPETVKKLVKAGASVAIRTGAGLRSH
                     FSDEDYKAAGAKIARTDAEAVADADIVLTVGRPRAEIVASMKKGAAVIGMLDPFADAD
                     GLDKLAKAGVALFAMELMPRISRAQNMDVLSSQSNLAGYKAVVDAAAAFERAVPMMMT
                     AAGTVPAARIFVMGVGVAGLQAIATARRLGAIVTATDVRPAVKEQVASLGAKFIAVED
                     EEFKQAETAGGYAKEMSDAYKKKQAALIAEHVKSQDIVITTALIPGRPAPKLISKEMI
                     ESMKPGSIIVDLAVERGGNAELAKPGEIVTHKGVRIFGKLNLPGEVPVNASSLYARNL
                     QAFVEPLIDKEKKTLAINWDDELVKGTLIARDGAIVNAMIAERLSAKRLGAEQADAPA
                     KSSGTKRQAKEAKS"
     gene            6214..6630
                     /locus_tag="VFY74_06420"
     CDS             6214..6630
                     /locus_tag="VFY74_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEX5931369.1"
                     /translation="MRINSRGVCLGALVLAVAATWPFAGTALAAEAASGGEQDFVFRV
                     SIFVLAIFVGYYVVWSVTPALHTPLMSVTNAISSVIVVGALLAVAVEAADAAGSSGHV
                     LAKLLGLAALIMASINIFGGFLVTQRMLGMYQKKKK"
     gene            6627..8021
                     /locus_tag="VFY74_06425"
     CDS             6627..8021
                     /locus_tag="VFY74_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5931370.1"
                     /translation="MSPNLVALLYLVAGVLFILALRGLSSPETSRRGNYLGMAGMAIA
                     VLTTLAVAQPKDPLSWAMILGGFAIGGGIGAAIARRIPMTAMPELVAAFHSLVGMAAV
                     LVAAAAFYAPEAFGIGTRGALRTASLIEMSLGAAIGAVTFTGSVIAFLKLSGRMSGKP
                     ILLPARHLINIALAVLLVVSVVMFCREQSAFYFWAIAALSFLLGVLIIVPIGGADMPV
                     VISMLNSYSGWAAAGIGFTLGNSALIITGALVGSSGAILSYIMCKGMNRSFISVILGG
                     FGGEVAGPAAGAEQKPVKLGSAEDAAFLMKNAGKVIVVPGYGMAVAQAQHALREMADG
                     LKQAGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFPQADVVYVIGA
                     NDVTNPSAEDDPTSPIYGMPVLEVWKAGTVLFNKRSLASGYAGIDNPLFYRDNTMMVL
                     GDAKKMTEEIAKSL"
     gene            8409..9278
                     /locus_tag="VFY74_06430"
     CDS             8409..9278
                     /locus_tag="VFY74_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5931371.1"
                     /translation="MVNLILALAGIFALIVGIGRLLHRYFIYLPDRARIDPKDAGLSG
                     VEEVVFKATDGTKLIAWYRPARGSKPTLLYFTGNSGNAASRAGKIRAITADDYGVFML
                     NYRRYGGSEGWPSETRITADALSAYDCLRGLGVAPHDIVAYGESLGTAVATRLSLQRH
                     VEALVLEAPFTSIVDVGRLMWKGFPLSLIMADKYRTIDRIGAVKVPLLIVHGGRDAVI
                     PLDQARHVFHAANEPKSMSVVPQAGHNDLFDRGAWERVRGFIDTLRPVPVKVRARGGR
                     RVEIAAAASGEAR"
     gene            complement(9428..9685)
                     /gene="rpsU"
                     /locus_tag="VFY74_06435"
     CDS             complement(9428..9685)
                     /gene="rpsU"
                     /locus_tag="VFY74_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5931372.1"
                     /translation="MQVVVRDNNVDQALRALKKKMQREGIFREMKLRNYYEKPSEKRA
                     REKAEAIRRARKLARKKAQREGGGGVGAAAAARPRAPRRSF"
     gene            complement(9828..10913)
                     /locus_tag="VFY74_06440"
     CDS             complement(9828..10913)
                     /locus_tag="VFY74_06440"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX5931373.1"
                     /translation="MPDSPLPPGSTIGILGGGQLGRMLAVAAARLGLKTHIYADETDA
                     PAFDVAAAQSVGSYADADAIARFAKSVDVVTCEFENVPAAALEAAAAAVPVFPQAKSF
                     AIAQDRLAEKDFVTGLGIAVAPYAPVGSREDLVSGLARVGLPALLKTRRLGYDGKGQV
                     PIRTGQDASAAFASLGAVPALVEGIVPFEREISVIVVRGQRGEMQFYDPVENTHQGGI
                     LATSRAPARIAPALSVEARTIAGKIVEALSHVGVICVEMFARSGGRPELRVNEFAPRV
                     HNSGHWTLDACITSQFENHVRAISGWPLGETARHSNALMTNLIGADIEKWRELAAEPD
                     TAVHLYGKAQAREGRKMGHVTRLYPKS"
     gene            complement(10906..11403)
                     /gene="purE"
                     /locus_tag="VFY74_06445"
     CDS             complement(10906..11403)
                     /gene="purE"
                     /locus_tag="VFY74_06445"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5931374.1"
                     /translation="MSDDKPVVGIIMGSRSDWPTLKAAAAVLDALKVAYETKVVSAHR
                     TPQRLYAYASSARERGLKVIIAGAGGAAHLPGMAASMTELPVLGVPVESRTLKGLDSL
                     LSIAQMPAGVPVGTLAIGEAGATNAGLLAAQIIALGDTRLAARVMAWRAAQTESVGEA
                     VEDNA"
     gene            complement(11498..11704)
                     /locus_tag="VFY74_06450"
     CDS             complement(11498..11704)
                     /locus_tag="VFY74_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX5931375.1"
                     /translation="MESEEIRELRATLLRLKQEHRDLDSAINALEESGRADALQLKRL
                     KKKKLFLKDEIARIEDQLLPDIIA"
     gene            11913..12089
                     /locus_tag="VFY74_06455"
     CDS             11913..12089
                     /locus_tag="VFY74_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX5931376.1"
                     /translation="MALDARLEELSEKHRALDRKIEEELARPTSDDLQIAEWKRQKLR
                     LKDEMERLKHMLSH"
     gene            12222..12581
                     /locus_tag="VFY74_06460"
     CDS             12222..12581
                     /locus_tag="VFY74_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5931377.1"
                     /translation="MRSDRSYQPLLGACLALVWLGLPAEAAEKDMVDRGEVLVKDNCS
                     RCHAIGREGDSPHKEAPPFRTLSAKYPVEELAESLAEGIVSGHPDMPIFVFGPQDVGA
                     IIDYLDSIQDKPAAEPQ"
BASE COUNT         2232 a         4022 c         4024 g         2375 t
ORIGIN      
        1 gagatgctga ccttccgttc gcttctcgac cagacctccg accgccgcga gcgcaaggcg
       61 atgctggcgc agaaagtcga ggacatgatc aacacggtgg tccgccagat cgccttttac
      121 agtttcgagc gcaaggtcca caccgagcgg cgcgacggcg agctgacgag cgaccgtctg
      181 ggcgaggtct ggctcgacgt gcagcgcgag agcctggggc cggccatcga tctcaagacg
      241 ggctacgaga cgttctggtg ctacatcccg catttcatcc acgccccgtt ttatgtctac
      301 gcctatgcct tcggcgattg cctggtgaac tcgctctatg cggtctacga gaaggccgac
      361 aagggcttcg ccgagcgcta tctcgacatg ctcgcggcgg gcggcaccaa gcatcacaag
      421 gagctgcttg cgccgttcgg gctcgatgcc tcggacccca atttctggtc gatgggtcta
      481 aaggtgatcg agggcttgat cgaccggctg gaaaccatcg accgcggcta aacgcgcctt
      541 tgccgctccc ccacccggcg ctcaagagcg cgcccagctt taggtatgaa gtttcgcgtc
      601 gacaagggct gagcgccgtc ctgttgtaca ttctgtataa ccatgttata cagaacgtct
      661 aacggagagc ggccatgaaa atcgagatca agcgtatcgg caattcggag ggcttcatcc
      721 tgccgcgcga gctgatgcag cgcctcgggc tgaagcgcgg gcagacgctg cacgtcactg
      781 agttggcggg aggcgggttc caagcgctcc cctatgatcc ggatttcgaa aagacgatgg
      841 agatcgtcga cgagatcatc gacgagtatc gcgacacgct ctcggcgctc gccaagtgac
      901 cgagcccaaa tggttcacgt atgaacaagc catcgccgcc catagcgctc agcttatccg
      961 ttatggcggc gcctctggcc tcagggacga gggcgcattg cgctcggcgt tggaccgtcc
     1021 cgtaaacaaa tggcactacg aacgcgctgg cttgcccgag cttgcggcgg cctacgcatt
     1081 tggtcttgcc aagaaccacc cctttgtcga cggcaacaag cgcatttcgt tcattgccat
     1141 ggtgacgttc ctccgcaaga acggcgtcgc gtttcgaccc gagcaggcgc agtccacggc
     1201 aatcattctc gctgtcgcgg ccggcgaggt gagcgaagaa agtttgacgc gctggattcg
     1261 cgataactgg ccgaagggct cttcatcgcc ttgacgcaag tcgcggttgt cgccagcgac
     1321 gctcgcgctc gaagccgagc tggaaatggt gcacaaggcg atgtgggcgt atcgcaacgc
     1381 cttaagcgag ctcggcaaac actagggctg catttgggct tgcggctcag tgcctggcaa
     1441 agatctcagt cgtgccgtct ttcttgatga gcagcacgtc gtagggctcg gtctttccat
     1501 cctgctccat tcccggggag ccgaccggca ttcccggcac ggtgagaccg agcgcgtcgg
     1561 gctgctcggc aagaagccgc tcgatgtccg ctgcgggcac atggccctcg atgacgtagg
     1621 cgccgatctt ggcggtgtga caggccgcaa gctgctcgcc gatcccggcc tcgcgcttga
     1681 tgcggtcgag catcgacggg atggcggcgt tgaccttaac gtcgaatccg ttcgtctgga
     1741 gatgctcgac ccacgcgccg cagcattcgc aactgggcga cttccacact tcggcccgcc
     1801 cttcggccgc ggcaaggtcc ggccaaatcc acgttatcgc cagaaagaac gcgagcactg
     1861 atcttggggt cataggctta tccttccggc gccgagaatg actctattgg caagcaggcg
     1921 caattcgccg caccggggtc agttatggtg ttggcatcgc aggcccctat atgaggagag
     1981 accacctcag ccgcgatccg tcatgaacga ccgccagtcc gataaggaaa agaaccggtt
     2041 cagcgcccgc gcgatccgtt atggcagggt cggcgccaat gtcggcaccg tcgcggcccg
     2101 cattgccgcg cggcgccttc tcgggctcga ccctgagcgc gaccgcgacg ccgcagcgct
     2161 tgccgccgct cttggcggcc tgaagggtcc gatcatgaag gtggctcagc tgctggccac
     2221 cattcccgag gcccttccgg ccgaatacgc cgccgagctt ggccagcttc agagccaagc
     2281 cccgccgatg ggccggtcct tcgtgcgccg gcgcatggcg gcggagcttg ggcccgactg
     2341 ggaaacgaaa ttcgcctcct ttgagcagga ggccgcggcg tcggcctcgc tcggcccagt
     2401 gcatcgcgcg gtcgctgacg gccgtgcgct tgcttgcaag cttcaatatc cggacatgca
     2461 gtcggcggag gaggccgatc tcgtccagct cgccgtcctc ttttccattc atgcgcgcat
     2521 gagccccgcc atcgacacct cggagatgct gaaggaaatc tcggcgcgct tacgcgagga
     2581 gctcgactac gatctcgaag cgcggcacac gcggctctac ggcgtcatct tcgaacgcga
     2641 cccgctcatt cgcgtacccg aagtgctcgc cgaactctcg accaagcggc tcctcaccat
     2701 gacctggctc gaaggaaggc ggctcctcga ctatacgtca cgcccgctcg aagagcgcaa
     2761 ccagatcgcc cgcgcaatgt tccgcgcctg gtggtacccg ttcagccatt acggagtgat
     2821 ccatggcgat cctcatctcg gcaattacac catcttcgag gacggcgagg gacgcacggc
     2881 cgggatcaac ctgctcgact atggctgcat gcgcaccttc gcccccaaat tcatccaagg
     2941 cgtggtcgac ctttaccagg ggctgctccg aaaagaccgt gcgctgatcg tgcatgccta
     3001 cgagacctgg ggcttcaccg gcctctccaa tgagctgatc gacatcctca atatctgggc
     3061 gaacttcatc tacggtccga tgctcgagga tcgcgtccgg accatcgccg aaaaagtgag
     3121 ccctggcagc tacggaaggc aagaagcctt caaggtcgct cgtggcttgc gggagagggg
     3181 gccggtgaag gtgccgcggg agttcgtgtt catggaccgg gctgcgatcg ggctcggcgg
     3241 ggtgttcctg catctcaatg cggaactcaa ctaccaccgc ctgttcaacg acacgatcgc
     3301 ggggagcgat ctcgccgatg tggccgggcg acagcgcgcg gccttcgggg cggccggcgt
     3361 gcctctgccc gaagaatcct aaaatccgag tagctttagg agcacaaatc catgcgtaaa
     3421 gcgaaggcat actctgctgc cagtgcgaca tcgtcactgg cctcggacac gattccacga
     3481 cgcgatccga ccgaacgcga cgtgcagatc gagattcttt tctgcggcat atgccattcc
     3541 gatctgcatc aagtccgcaa cgagtggagc ggcgtgatgc cgacggtcta cccttgcgtc
     3601 cctgggcatg agatcgtcgg ccgcgtcacc ggggtcggcg cggcggttag caaatacaag
     3661 cctggcgacc tcgtggcggt cggctgcatg gtcgattccg accgcacctg cccgcattgc
     3721 cgggagggcc ttgagcagtt ctgcccgagc atgatcctca cctacaattc gcccgacaag
     3781 catctcggag gcgtgaccta cggcggctac tccgagagca ttgtcgtcga cgagcatttc
     3841 gtcttgcgcg tcccttccaa tctcgatctc gccggggccg cgcccctgct ctgcgcaggg
     3901 atcacgacat actctcccat gcgccactgg ggcgttagcg cgggcaaaaa ggttggcgtg
     3961 gtcggtctcg gcggattggg tcatatgggc gtgaagttcg ctcacgcctt cggagcccac
     4021 gtcgtcgtct tcaccacgtc ccctgggaag aaggacgccg cgctcggcct cggcgccgac
     4081 gaagtggtcg tctcgcgcaa cgccgacgag atgaagaagc acgccggcag cttcgacttc
     4141 atcctcgaca ccgtttccgc cgaccacgac atcaacgcct acctcaacct gctgcggcgc
     4201 gacggcaata tggtgcttgt cggcgctccc gaggaatttc tcggcgtgtt ggcgttcagc
     4261 ctgcttgttg ggcgccgcag tctgtccggc tccatcatcg gcggcatcgc cgaaacgcag
     4321 gagatgctcg acttttgtgg agaacacaac atcacggccg atgtcgaagt cattcctatc
     4381 cagaaggtca acgaagccta cgagaggctg ctcaagtcag acgtgaagta ccgcttctcc
     4441 atcgatatgg cgtcgctcaa atccgggtaa ctgccgcggc gggcgtgcct cggtcctgaa
     4501 ggttcgtagc gtggcttctg tcaaacctcc ccctcgtggg gaggtcggcg cgctattcca
     4561 tttttgcgct accactccgc tgcttgagcg catagcgcgc cgggtggggg gtgtttttct
     4621 cggatcatcc ggcccggagc ttcaggccaa tgccgttacg cggcgagtgc cgattattgc
     4681 ccgcccgcgg ccctttatgc tattggcgca gggagagcca taatgcgtca ggggagtgca
     4741 atgcggatcg atccgaaaga gggcagcccg gcaatggatt atgccgagca catgaagacc
     4801 tacaaattgt tttgcaagct cacggccttc gccatcctgg gcgcggttgt ggtcatggtg
     4861 cttcttgcct tgtttgtggc ctgagcggcc agatctttcg cctggcggcg atgttgcccc
     4921 gccgcttctt tcagcccctc gacatcgacc gcaccggcgc ttacgcccgg cgcattcttg
     4981 agcgtctagg accacgatga tcgcaattgg cgtgcccgcc gaaagtgaag acgagccgcg
     5041 catcgggctc acgccggaaa cggtcaagaa gctcgtcaag gccggcgcga gcgtcgccat
     5101 ccgcacaggc gccgggttgc gttcccactt ttccgacgag gactacaagg cggccggcgc
     5161 caagatcgcc aggaccgacg ccgaggctgt cgccgatgcc gacatcgtat tgaccgtcgg
     5221 gcgtccgcgc gcggaaatcg tcgccagcat gaagaaaggc gcggcggtga tcggcatgct
     5281 cgatccgttc gccgatgccg acggtctcga taagctcgcg aaggcgggcg tcgctctctt
     5341 cgccatggag ttgatgccgc gcatctcccg cgcccagaac atggacgtgc tgtcgtcgca
     5401 atcgaacctc gcgggctaca aggccgtggt ggatgcggcc gccgcattcg agcgcgcggt
     5461 gccgatgatg atgacggccg cgggcacggt gcccgccgcg cgcatcttcg tcatgggcgt
     5521 cggcgtcgcc ggcctccagg ccatcgccac ggcgcgccgg ctcggggcca tcgtcaccgc
     5581 caccgacgtg cgcccggcgg tcaaggaaca ggtggcctcg ctcggcgcca agttcatcgc
     5641 ggtggaggac gaggagttca agcaggccga gaccgcaggc ggctacgcca aggaaatgtc
     5701 ggacgcctac aagaagaagc aggcggcgct cattgccgag cacgtcaaga gccaggacat
     5761 cgtcatcacg acggcgctga ttccgggccg gcctgcgccc aagctcatct ccaaggagat
     5821 gatcgagagc atgaagcccg gctcgatcat cgtcgatctc gcggtcgagc gcggaggaaa
     5881 cgccgagctc gcaaagcctg gcgagatcgt cacgcacaag ggcgtgcgca tttttggcaa
     5941 gctcaatctt ccgggcgagg tgccggtcaa cgcatcgagc ctttacgcgc gcaaccttca
     6001 ggccttcgtc gagcctctga tcgacaagga gaagaagacg ctcgccatca attgggacga
     6061 cgagctggtc aagggcacgc tcatcgcgcg cgacggtgcc atcgtaaacg ccatgatcgc
     6121 cgagcgtctc agcgcgaagc gtctcggcgc ggaacaggcg gatgccccgg caaagtcaag
     6181 tggcaccaag cgccaagcga aggaggccaa gtcatgagga tcaattcgcg cggcgtttgt
     6241 ctcggcgcac ttgtcttggc cgtggccgcc acatggcctt ttgccgggac ggcactggcg
     6301 gcggaggcag cgtcaggggg cgaacaggat ttcgtctttc gcgtctcgat tttcgttctc
     6361 gccatcttcg tcggctatta cgtggtgtgg agcgtgacgc ccgccctgca cacgccgctg
     6421 atgtcggtga ccaacgccat ctcgtcggtg atcgtcgtcg gcgcgctcct tgccgtggcg
     6481 gtcgaggcgg ccgatgcggc cggctcctcg ggtcatgtcc ttgccaagct gctcggtctt
     6541 gcggcgctga tcatggcgag catcaatata ttcggcggct tcctcgtcac ccagcgcatg
     6601 ctcggcatgt atcagaagaa aaagaaatga gcccgaacct cgtcgcgctg ctctatctcg
     6661 tcgcgggcgt cctcttcatc ctcgccctgc gcggactgtc ctcgccggaa acatcgcggc
     6721 gcgggaatta tctcggcatg gccgggatgg ccattgccgt gctcaccacg ctcgccgtgg
     6781 cccagccgaa agaccctctg agctgggcca tgatcctggg tggctttgcc atcggcggcg
     6841 gcatcggggc tgcgatcgcg cggcgcatcc ccatgacggc gatgcccgag cttgtcgccg
     6901 cgttccactc gctcgtcggc atggcggcgg tgctggtcgc ggccgcggcc ttttacgcgc
     6961 ccgaggcgtt cggcatcggg acgcggggcg ccctccgcac ggcgagcctg atcgagatgt
     7021 cgctcggcgc ggccatcggg gccgtcacct tcaccggatc ggtcatcgcg ttcttgaaac
     7081 tgtcggggcg catgtcgggc aagcccatcc ttctccctgc gcgtcatctc atcaatatcg
     7141 cgcttgcggt cttgctcgtc gtctctgtcg tcatgttctg ccgcgagcag agcgcgttct
     7201 acttctgggc gatcgcggcg ttgtccttcc tgctcggcgt cctcatcatc gttcccatcg
     7261 gcggcgccga tatgccggtc gtcatctcga tgctgaactc ctattcgggc tgggcggcgg
     7321 ccggcatcgg cttcacgctc ggcaattcgg cgctcatcat caccggcgcg ctcgtcggct
     7381 cctctggcgc gatcctctcc tacatcatgt gcaaggggat gaaccgctcc ttcatcagcg
     7441 tgatcctcgg cgggttcggc ggcgaggtgg cggggcccgc cgcgggcgcc gagcagaagc
     7501 cggtgaagct cggctcggcc gaagatgcgg ccttcctcat gaagaacgcc ggcaaagtca
     7561 tcgttgtgcc gggctacggc atggcggttg cgcaggcgca gcacgcgttg cgcgagatgg
     7621 ccgacgggct gaagcaggcg ggtgtcgagg tgaaatatgc aattcatccg gtggcggggc
     7681 gcatgcccgg ccacatgaat gtgctgctcg ccgaagcgaa tgtaccctat gacgaggtgt
     7741 ttgagctcga ggacatcaat tcggaatttc cgcaggccga cgtcgtctac gtcatcggcg
     7801 ccaatgacgt caccaatccc tccgccgaag acgatccgac ctcgccgatc tacggcatgc
     7861 cggtgctcga agtctggaag gcggggaccg tgctgttcaa caagcggtcg cttgcctcgg
     7921 gctatgccgg catcgacaat ccgttgttct atcgcgacaa caccatgatg gtgctcggcg
     7981 acgccaagaa gatgaccgag gaaatcgcca agtcattgtg atgggcccct gcggtcaatc
     8041 gccgcaaatc aatcgaagaa tcgcccaatt gcggctcgat tcggccgtta ggaccgctcc
     8101 cgcacggaga ggatatctcc gtaagtcttg ccttaagagc cggagcaggg gatacgtccc
     8161 gcaccgtgaa cttttcttaa gcatacggct tctataggtg cagggccggt gggtcccgcc
     8221 ggttcatgac gcgccggatt tggcgcaagg gccttggtgc tccgcacctt cccatcgtcg
     8281 agatcagccg caaatcgaga ggagtttgag cctatatgaa agtgtcattc aaacggcgtt
     8341 catgaatgtg aacaattaag tccaagcgca gctttaaacg aaacgtctcg aagggtattg
     8401 cttgaaccat ggtgaacctc attctcgcac ttgccggcat cttcgcgttg atcgtcggca
     8461 tcggccggct gctgcaccgt tatttcatct atttgccgga ccgcgcgcgc atcgatccaa
     8521 aggacgcggg gctttctggt gtcgaagagg tggtcttcaa ggcgaccgac ggcaccaagc
     8581 tgatcgcctg gtatcggccg gcacgcgggt cgaagccgac acttctctac tttacgggca
     8641 atagcgggaa cgcggccagt cgcgccggca agatcagggc gatcacggcc gacgattacg
     8701 gtgtgttcat gctgaactat cgccgctatg ggggttccga gggttggccg agcgagacaa
     8761 gaatcacggc cgacgcgctc agcgcctatg attgcttgcg cgggcttggt gtggccccgc
     8821 acgacatcgt cgcctatggc gaatcgctcg gcacggcggt cgcaaccagg ctatccctcc
     8881 agcgccatgt cgaagcgctc gtgctcgaag cgccgttcac ctcgatcgtc gatgtcggga
     8941 gactcatgtg gaagggattc ccgctcagcc tgatcatggc cgacaagtat cgcaccatcg
     9001 atcggatcgg cgctgtcaag gtgccgctct tgatcgttca tggcggccgc gacgcggtca
     9061 tccctctcga tcaggcgcgg cacgtgtttc atgccgccaa cgaaccgaag agcatgtcgg
     9121 tcgtgccgca ggctggccat aacgacctgt tcgatcgggg cgcctgggag cgagtgcgcg
     9181 gctttatcga cactctgcgg cccgtgccgg tcaaggtcag ggcgaggggc ggacgtcggg
     9241 tcgagatcgc ggcggcggca agcggcgaag cccgctagga acgccaacaa tccgtcgggc
     9301 gtgttggcga ccgccgctga tcctcaaaaa cgctaatggc cggggcctgc ctacgcgaag
     9361 ccacgcttcg ctctggcaag gcaggcgggc ccggccatgt cggcaatcta aggcagtttg
     9421 gaggctgcta gaacgaccgg cgcggggcgc gtggtctcgc ggcagccgca gcgccaacac
     9481 cgccgccgcc ctcgcgctgc gccttcttgc gcgcgagctt ccgcgcgcgg cggatggcct
     9541 cggccttctc gcgggcgcgc ttctctgacg gcttttcgta gtagttccgg agcttcatct
     9601 cgcggaagat gccttcgcgc tgcatctttt tcttcagggc cctcaaggcc tgatcgacat
     9661 tgttatcgcg aacgacgact tgcacgagct tttctctctc cgcttggccg aaggattgtc
     9721 aggatggggg cgggaggcca aatcagcccc ggccggaacc ggtcagggtc cccgcgtccg
     9781 caggtgctct aacagaagcc ccgcgcattg tccatatcgg gaaggcgcta actcttcggg
     9841 tagagccggg tgacgtggcc catcttgcgg ccctcgcgcg cttgcgcctt gccgtagaga
     9901 tggacggcgg tgtcgggctc ggcggcaagc tcgcgccatt tctcgatatc ggcgccgatc
     9961 agattggtca tcagcgcgtt gctgtggcgc gcggtctcgc cgagaggcca gcccgagatt
    10021 gcgcggacgt ggttctcgaa ttggctggtg atgcaggcat cgagcgtcca atgccccgaa
    10081 ttgtgcaccc gcggggcgaa ctcgttgacg cgcaactcgg gccgcccgcc actgcgggcg
    10141 aacatctcga cgcagatcac cccgacatgt gagagcgcct cgacaatctt gcccgcgatg
    10201 gtcctcgcct cgacgctcaa ggccggcgcg atgcgcgccg gtgcccggct cgtggcgaga
    10261 atgcccccct gatgggtgtt ctcgaccgga tcgtagaact gcatctcgcc cctctgtccc
    10321 ctgacgacga tcaccgaaat ctcgcgctcg aagggcacga ttccttcgac cagcgccggc
    10381 acggcgccga ggcttgcgaa tgcggctgag gcgtcctgcc cggtgcggat tggaacctgc
    10441 cccttgccat catatccgag gcgcctggtc ttgagcaggg cagggagccc cacgcgcgcg
    10501 agcccgctca cgagatcctc gcgcgaaccg accggggcat agggagccac cgcaatgccg
    10561 agcccggtta cgaagtcttt ctcggccagc cggtcctggg cgatggcgaa cgacttcgcc
    10621 tgaggaaaga cgggaacggc tgctgcggcg gcttcgagcg cggccgccgg cacgttctcg
    10681 aactcgcacg tgaccacgtc gacgcttttc gcgaagcgtg cgatcgcatc ggcgtccgca
    10741 taggagccga ccgactgcgc cgccgccaca tcaaaggccg gcgcatcggt ttcatcggca
    10801 tagatatgag tcttgaggcc gagtctggcg gcagccacgg cgagcatgcg gccgagctgc
    10861 ccgcctccga ggatgccgat cgtgctgccg ggcggcagcg ggctgtcagg cattatcctc
    10921 gaccgcctcg ccaactgatt cggtttgcgc cgcgcgccag gccatgacgc gggcggcaag
    10981 acgcgtatcg ccaagcgcga tgatctgggc cgcgagcagc cccgcgtttg tcgcgccggc
    11041 ctcgccgatc gcaagcgttc cgaccggcac gccggcgggc atctgggcga tggagaggag
    11101 actatcgagg cccttcaacg tgcggctctc gaccgggacg ccgaggaccg gcagctcggt
    11161 catcgaggca gccatgccgg gcaggtgggc ggcgccgccc gcgccggcga tgatcacttt
    11221 cagcccgcgc tcgcgcgcgc tcgaggcgta ggcgtaaagc cgctgcggcg tgcggtgcgc
    11281 ggagacgacc ttggtctcgt aggcgacctt gagcgcatcg agcacggcgg ccgcggcctt
    11341 cagtgtcggc cagtcggagc ggctccccat gatgatgccg acgacgggct tatcatcgct
    11401 catgtgcgat tctctcggaa aaaacgcggc ttggcggcgg aaagcgcggg attataggga
    11461 cggccctctc cttggcaagg cacgttctcc ggtctggtca ggcgatgatg tcggggagga
    11521 gctggtcctc gatgcgagcg atctcgtcct tcaggaagag cttcttcttc ttgaggcgct
    11581 tgagctgaag ggcgtcggcg cggcccgatt cctccagcgc gttgatggcg ctgtcgaggt
    11641 cgcgatgctc ttgcttcagc ctgagcagcg tcgcccggag ttcgcgaatc tcctcgctct
    11701 ccatcggacg tcccctgctc cttgcctctt tccgggacgg cagaaagtat cgcggcgaac
    11761 cgggaaccgc gcaaggcctt ggcgcgattt gccaagggtt taagcggttc gaccgtctcg
    11821 cgcgtaacgc gcgagtgagg accacaatta gacaaatcgc tgcgaatttg tcacaatgga
    11881 caaaccgaga ccaactgtca tggaggtccg atatggcgct agacgcacgc ctcgaggagt
    11941 tgtccgaaaa gcatcgtgca ctggatcgaa aaatcgaaga agagttggcg cggccaacct
    12001 cggacgacct gcagatcgcg gaatggaagc gtcaaaaatt gcgacttaag gatgagatgg
    12061 agcgtctcaa gcatatgttg agccattagg gctcgcgctc aagagtttcg aaggccgcgg
    12121 gggcgcaagc ttcgcttgcg ccgaagcggc ctttcatttt cggtatttca tgcccgatcc
    12181 ggtgccggcc gcgctataac tccccgggga ggggccgcga aatgcgaagc gatcgatcgt
    12241 accagccgct ccttggcgct tgcctcgcgc tcgtttggct tggtctcccg gctgaggcgg
    12301 ccgagaaaga catggtcgac cggggcgagg tccttgtcaa agacaactgc tcgcgctgcc
    12361 atgccatcgg cagggagggc gacagtccgc acaaggaagc gccgccgttc cggacccttt
    12421 cagccaagta cccggtcgaa gaactggccg agtcactcgc cgagggcatc gtctcgggcc
    12481 acccggacat gccgatcttc gtgttcggcc cacaagacgt cggggcgatc atcgactatc
    12541 tcgattcgat ccaggacaag cctgccgccg agccgcaata ggcgcggttg cgccgccggg
    12601 cagagcgctc ccgttgggaa aaacgagcgt gaccgtgttg acttatctct tgt
//
LOCUS       DASUQP010000145         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_210040,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000145 DASUQP010000000
VERSION     DASUQP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210040"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..297)
                     /gene="gatC"
                     /locus_tag="VFY74_06465"
     CDS             complement(10..297)
                     /gene="gatC"
                     /locus_tag="VFY74_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5931378.1"
                     /translation="MSVDRATVRRIARLARLAITDEEESRLEQELSGILDWVAQLDEI
                     DTSAVAPMTRVAAMTMKMREDKVTDGFCAADILKNAPQVDDGYFVVPKIVE"
     gene            complement(374..1075)
                     /locus_tag="VFY74_06470"
     CDS             complement(374..1075)
                     /locus_tag="VFY74_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5931379.1"
                     /translation="MQITWFGHSCFRIDTGASRILIDPFLGGNATFEKAGIPVHKAID
                     GVTHVALTHGHEDHTGDTLRVCKETGAELLAVHELATYLAGQGVEKIDAGNTGGTLHH
                     GDFDLTFVPALHSSSHMVDGRPVYLGACCGLVIAPRGERTVYHMGDTAIFSDMGLVGE
                     IHAPQIGFVPIGDRYTMGGKAAALACKRFFRFEMIVPCHYGTFPVLDQTADQFIAAFG
                     SDRVLVPEVGVAFEA"
     gene            1103..1588
                     /gene="ruvX"
                     /locus_tag="VFY74_06475"
     CDS             1103..1588
                     /gene="ruvX"
                     /locus_tag="VFY74_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5931380.1"
                     /translation="MTVAPLVAIEDLPGWLAPDARLLCLDLGTKTIGLALSDVTRSVA
                     TPYRTLRRTKFTGDAKAIAAMIAENGVGALVIGLPINLDGSEGPRAQATRAFARNLAS
                     FIAVPILLWDERLSTAAVERHLIEAGASRKRRAEVIDRMAAAYILQGALDRLKRLGRR
                     S"
     gene            complement(1585..2124)
                     /locus_tag="VFY74_06480"
     CDS             complement(1585..2124)
                     /locus_tag="VFY74_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX5931381.1"
                     /translation="MPSDLSRLLNALGLVAIDTVLALALIDQLWFRDLPCPICILQRV
                     AFIAAGYGIALNLLFGPRPSHYGMTIIGAAVGGAIALRQILVHIVPGSGSYGNAVFGM
                     HLYTWAFICFTLIIVGTAVLLFYERQFTRSEPWSSRLDVLALTTLATFILLAVVNIFS
                     TLLLCGFGLCPDAPTGYLI"
     gene            complement(2140..>2331)
                     /locus_tag="VFY74_06485"
     CDS             complement(2140..>2331)
                     /locus_tag="VFY74_06485"
                     /inference="COORDINATES: protein motif:HMM:NF039621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5993 family protein"
                     /protein_id="HEX5931382.1"
                     /translation="CLRSPACWRSRTMMSLPFILFACGLGAAWLRQRGAAVGFWVAGV
                     VVLLVLFRAHSGDALNIAL"
BASE COUNT          462 a          740 c          758 g          371 t
ORIGIN      
        1 tgctccggtc tactcgacga tcttgggcac cacgaaatag ccgtcgtcga cttggggggc
       61 gttcttcaag atatcggcgg cacagaagcc gtcggtgact ttgtcttcac gcatcttcat
      121 ggtcatggcc gccacccgcg tcatcggcgc aacggcgctc gtatcgatct cgtcgagctg
      181 ggccacccaa tcgaggatgc cggagagttc ctgctcaagc cggctctcct cctcgtcggt
      241 aatcgcaagc cgggcaaggc gagcaatgcg gcggacggtg gcgcgatcga cagacatggg
      301 acccaaggct tcggctggtt tcgttgagcg ctcgtctagc accgtgccgc ccaagcggca
      361 acttgggtcg aggtcatgcc tcgaacgcga cgcccacctc aggcaccaac accctgtcgc
      421 ttccgaacgc ggcgatgaat tggtcggcgg tctggtccag caccgggaag gtcccgtaat
      481 ggcagggaac gatcatctcg aaacgaaaga agcgcttgca ggcgagcgcc gccgcctttc
      541 cccccatggt gtagcggtcg ccgatcggca cgaagccgat ctgcggcgca tggatttcgc
      601 caacgagccc catgtcggag aagatcgccg tatcgcccat gtggtagacg gtcctttccc
      661 ctctcggggc gatgacgagc ccgcaacagg cgccgagata gaccggacgc ccatcgacca
      721 tatgcgagga agaatgcagg gccggcacga aggtcaggtc gaaatcgccg tggtggagcg
      781 tgccgccggt gttaccggca tcgattttct ccactccttg gccggcgaga taggtcgcaa
      841 gctcgtgcac ggcgaggagc tcggcgcccg tctccttgca gacgcgcagc gtatccccgg
      901 tatggtcctc gtgcccgtga gtgagcgcca catgggtcac gccgtcgatc gccttgtgga
      961 cggggatccc cgccttctcg aaggtggcgt tgccgccaag aaacggatcg atcaggatgc
     1021 ggcttgcgcc ggtatcgatc cggaagcagg aatggccgaa ccaggtaatc tgcatgcgtc
     1081 gtagccctcc gatttcattc tcgtgactgt agcacctctc gttgccatcg aagacctgcc
     1141 cggctggctc gcgcctgacg cgcggctgct ctgtctcgac ctcggcacca agaccatcgg
     1201 cctggcgtta tcggacgtga cgcgctccgt cgcaacgccc taccggacgc tgcgacggac
     1261 caagttcacg ggcgatgcca aggcgatcgc cgccatgatc gcggagaacg gggtgggcgc
     1321 cctcgtgatc ggccttccga tcaacctcga cggctctgag ggacccaggg cgcaggcgac
     1381 gcgcgcattc gcgcgcaacc tcgccagctt catcgccgtg ccgattctcc tctgggacga
     1441 gcggctgtcg accgcggccg tcgaacggca tctgatcgag gccggcgcct cccgcaagcg
     1501 gcgcgccgaa gtcatcgacc gcatggcggc ggcctacatc cttcaaggcg cgctcgatcg
     1561 gctgaagcgg ctcgggcgca gaagctagat cagatagccc gtcggtgcat ccgggcagag
     1621 gccgaagccg cagagcaaca gagtcgagaa gatattgacg accgcaagca gaatgaatgt
     1681 ggcgagagtc gtgagcgcca ggacgtcgag tcggctcgac cacggttcgg agcgggtgaa
     1741 ttgccgctcg tagaacagga ggacggcggt gccgacgatg atcagcgtaa agcagatgaa
     1801 cgcccaggta taaaggtgca tgccaaagac ggcatttccg tatgagccgc tgcccgggac
     1861 gatgtggacc aggatctggc gtaacgcgat agcgccgccg accgccgcgc ctatgatcgt
     1921 catgccgtaa tggctcggcc gcgggccaaa caacaggttg agcgcaatgc cgtagcccgc
     1981 cgcgatgaag gcgacccgtt gcagaatgca gatcgggcac ggcaagtcgc ggaaccagag
     2041 ctggtcgatg agggcaaggg cgagcaccgt gtcgatcgcg acgagcccca aggcattgag
     2101 aagccgcgac aggtcactcg gcactgtgtt cccccggcct caaagcgcga tgttgagagc
     2161 gtcgccggaa tgggcgcgga acaagacgag aaggacgacg acgcccgcga cccaaaaacc
     2221 gacagccgcc ccgcgctgcc gaagccaggc cgcgccaagt ccgcacgcaa agaggataaa
     2281 cggtaagctc atcatggtgc gactccgcca gcaggccggt gaccggagac a
//
LOCUS       DASUQP010000146         5651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_210493,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000146 DASUQP010000000
VERSION     DASUQP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210493"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..618)
                     /locus_tag="VFY74_06490"
     CDS             complement(82..618)
                     /locus_tag="VFY74_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931383.1"
                     /translation="MSRIELLRLVAGLVLLFVAGCGLRPSGFGGAWLGSDSSIKTANE
                     STSAVAAAAPGRMPIAVLKPVDAYVLLGGRIKSCWFNAVDPLLPNHVYQADVSPSGRM
                     VQIVIHEKLALGRAGNATYAVDFKEVDGYTVVRTFNRKMPPVLAAKMQYDIDRWKAGE
                     TNCSRVMPKVAAAAPAAQ"
     gene            complement(636..1121)
                     /locus_tag="VFY74_06495"
     CDS             complement(636..1121)
                     /locus_tag="VFY74_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5931384.1"
                     /translation="MPTFDVVSKTDLAEVDNALANMTREMSQRYDFKNSIARIERNGA
                     EITLNADDDLKLKQMHELLQGHLARRKVDARVIDYKPIEKAAGQAVRQKAVIRQGIDR
                     ELAKRLVKEVKDGKFKVQVTVQGDELRVSGKKRDDLQSVIQHLKGLSVEQPLQYVNFR
                     D"
     gene            complement(1177..1581)
                     /locus_tag="VFY74_06500"
     CDS             complement(1177..1581)
                     /locus_tag="VFY74_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX5931385.1"
                     /translation="MKSRDALIRLKRFEVEEKRRKVVEIETMIGDFNHMVSDLDRQIA
                     IEQERAGVSDVKHYAYPTFAKAAIQRRDNLAVSAANLAAKLAAARGDLDDACEELKKI
                     ELLEERDAERVRLEQARTAPDLDVAAIQRAAS"
     gene            2005..2700
                     /locus_tag="VFY74_06505"
     CDS             2005..2700
                     /locus_tag="VFY74_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5931386.1"
                     /translation="MRVLLIEDDSATAQSIELMLQSEGFNVYKTDLGEEGVDLGKIYD
                     YDIILLDLNLPDMSGYEVLKTLRVSKVQTPILILSGMAGIEDKVRGLGFGADDYMTKP
                     FHKDELVARIHAIVRRSKGHAQSVIQTGGLKVNLDTKTVDVNGQRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAAATGGKHYIETVWGRG
                     YVLRNPEDESEAA"
     gene            complement(3064..3429)
                     /locus_tag="VFY74_06510"
     CDS             complement(3064..3429)
                     /locus_tag="VFY74_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5931387.1"
                     /translation="MKHCLVVDDSAVIRKVARRILEELSFTASEAENGRDALDQCRAS
                     MPDVVLLDWNMPVMDGIAFLVALRRERGGSAPKVIFCTTENDAAHIARAINAGANEYI
                     MKPFDRDIVEAKFHQVGLI"
     gene            complement(3455..4093)
                     /locus_tag="VFY74_06515"
     CDS             complement(3455..4093)
                     /locus_tag="VFY74_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEX5931388.1"
                     /translation="MTEPAALSDLELAALVSSRICHDVINPVAAIANGLEMLGDEPDE
                     AMREAAMDLIRKSAAQASAKLQFARLAYGAAGSAGAEIDLRDAEKVARDFVRWTGKHQ
                     VNWRGPAATLPKNKVKLLLNLVALGLVALPRGGTIDVEIGGTPPAASFLVKATGDAAR
                     LADQVRSLLSGADSVTIDTHSIQPYYAKRVAVASGMAVTAEAREGEVAFSAA"
     gene            4241..5095
                     /gene="cysQ"
                     /locus_tag="VFY74_06520"
     CDS             4241..5095
                     /gene="cysQ"
                     /locus_tag="VFY74_06520"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX5931389.1"
                     /translation="MLPPRETTARAKALGSLGFDFGRVASRLTDAAVRAGAAIMRHCH
                     GDPEVQLKGDASPVTRADRNSEAIILAALAQFAPEVKVICEETFGGASGPLPHRFFLV
                     DPLDGTKEFIQKRSDFTINIALIEGGRPRFGLVYAPARALLAVTIEAGKAVEVELPPN
                     EAGADLDRLNPKPIHARVAENSGLTALVSHSHLDPETEAFLAKLNIAERVGVGSSIKF
                     VAIARGEADVYPRFGPTMEWDTAAGQAVLEAAGGRVVDTNDEPLRYGKTEQSLRNPSF
                     VAWGRGSS"
     gene            5249..>5651
                     /locus_tag="VFY74_06525"
     CDS             5249..>5651
                     /locus_tag="VFY74_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931390.1"
                     /translation="MHLRVSASAVLIALVSVLAPAAAQDRGESGSGIRLAQAEGPMVI
                     TPQPYSGQPGSYPPPQQYSDQPGSYPPPQPYSGQPGSYPPPQPYSGQPGSYPPPPNGY
                     DAGGPGAPYEPAPQGGAYVPRSGGYSSQAAGD"
BASE COUNT          991 a         1844 c         1761 g         1055 t
ORIGIN      
        1 aagcacggcc tccccggctt gcgcgcaacc cgcattcagg aatatcgcgg cagctagtcc
       61 gctaggccgc ggctgctccg tttactgcgc ggcgggagcc gccgcagcca ctttgggcat
      121 caccctgctg caattcgttt cgccggcctt ccagcggtcg atgtcgtatt gcatcttggc
      181 tgcaaggacg ggcggcatct tccggttaaa ggtccggacc accgtgtagc cgtccacctc
      241 cttgaaatcg acggcgtagg tcgcgtttcc ggcgcggccg agcgcgagct tctcgtggat
      301 cacgatctgc accatgcggc cgctcgggct gacatcggcc tggtagacat ggttcggcag
      361 aaggggatcg acggcattga accagcagct cttgatgcgg ccgccgagga gaacgtaggc
      421 atcgaccggc ttcaacaccg cgatcggcat ccgccccggc gccgcggccg cgaccgcgct
      481 cgtgctttcg ttcgcggttt tgatcgagga atcgcttccg agccaggcac cgccgaagcc
      541 gctcggacgc aagccgcatc cggcgacgaa gaggagcacg agacccgcga caaggcgcaa
      601 taactcgata cgtgacaagg tccccgtcct accctctagt cgcggaagtt cacatattgc
      661 aggggctgct cgacagagag ccctttcagg tgctggatga cggactgcag atcgtcgcgc
      721 ttcttgcccg acaccctgag ctcgtcgccc tgcacggtca cctgcacctt gaacttgccg
      781 tccttgacct ctttcaccag ccgcttggcc agctcccgat cgatcccctg tcggatcaca
      841 gccttctgcc gcaccgcctg cccggcggcc ttctcaatcg gcttatagtc gatcacgcgg
      901 gcgtcgacct tgcggcgggc gagatgacct tgcaggagct cgtgcatctg cttgagcttg
      961 agatcgtcgt cggcgttgag ggtgatttcc gccccgtttc gctcgatccg cgcgatcgaa
     1021 ttcttgaagt catagcgctg gctcatctcg cgcgtcatgt tggcgagcgc attgtcgacc
     1081 tcggcgagat cggtcttcga aaccacgtcg aaagtgggca ttgttagcct ccgcggccct
     1141 ccaagccgga gagccgaggc ccctgagcct aggctctcag cttgccgcgc gctggatcgc
     1201 ggccacgtcg aggtcaggcg cggtccgggc ctgctcgagc cgcacccgct cggcgtcgcg
     1261 ctcctcgagg agctcgatct tcttgagctc ttcgcaggca tcatcgagat cgccgcgggc
     1321 ggcggcaagc ttcgccgcga ggttcgcggc agacaccgcc aggttgtcgc gccgctggat
     1381 cgcggccttg gcaaaggtag gataggcgta gtgcttgacg tcagagacgc ccgcgcgctc
     1441 ctgctcgatg gcgatctgcc ggtcgagatc ggagaccatg tgattgaagt cgccgatcat
     1501 agtttcgatc tcgacgactt tccggcgctt ctcctcgact tcgaagcgct tcaggcgaat
     1561 caatgcgtcg cgtgacttca taactcggta ccctcgaaca tttcacaacc cgttctcgca
     1621 aaactcaata cccatctcgg gttagccgcc tccggccgtt gggctggcat cccgattccg
     1681 ggcgacggcg gctcttccgg gacggaatgt acgcctcgga aagggtcaca gccctgggca
     1741 ggaccggggg cccctgccct caaggccgat catcgccaat aggacttaag ggccggtaaa
     1801 ccatatggct cgcatttaac cgatgccgga gacgggcgcc gcgacctgat ggttagcggt
     1861 gcctgaacgg atggatggca ctcgccatcg cgatagcttt tgttaactat tggccactaa
     1921 tttgttgatt cggattaacc aatcggcgtg gcgtttcagg gtcggggaac aagccgggac
     1981 tgcgaggaga cgggggattt cactatgcgg gttcttctga tcgaggacga cagcgcgacc
     2041 gcgcaaagca ttgagctgat gctgcagtcg gaaggcttca acgtctacaa gactgacctc
     2101 ggggaagagg gcgtcgacct cggcaagatc tacgactacg acattattct acttgacctg
     2161 aacttgccgg acatgagcgg atacgaagtg ctcaagacgc tccgggtcag caaggtgcag
     2221 acgccgatcc tgatcctttc gggcatggcc gggatcgagg acaaggtgcg cggcctcggc
     2281 ttcggcgccg acgactatat gaccaagccc ttccacaagg acgagctggt cgcccgcatc
     2341 cacgccatcg tgcgccgctc aaagggccat gcccagtcgg tgatccagac gggcgggctc
     2401 aaggtcaatc tggataccaa gaccgtcgac gtgaacggcc agcgcgtgca tctgaccggc
     2461 aaggaatatc agatgctgga gctgctttcg ctgcgcaagg gcacgacgct gaccaaggag
     2521 atgttcctga accatctcta tggcggcatg gacgagccgg agctcaagat catcgacgtc
     2581 ttcatctgca agctgaggaa gaagctcgcg gccgcgaccg gcggcaagca ctatatcgag
     2641 acggtctggg gccgcggcta cgtgctgcgc aaccccgagg acgagtccga agccgcctga
     2701 cgcctctctc gttcccagaa caagaacgcg atggccgggg tccctccccg gccatttgct
     2761 tttttttccg ctctcccctt gagggagagc acgcgagcgc attttcgcgc tcgcggtaag
     2821 gggtcagccg cgaagcggcg tcaaaaaaat cacccctcac ccttcccgcc attcgccgcg
     2881 cttcgccacg tcgtggcttc gcgcggcggg cggaaagccc tctccctcat ggggagaggg
     2941 aaattgagtg catgcttcga gacgcgcttc gggcgctcct cagcatgagg gccgaggtgt
     3001 ggtgatgagc ctcaccctga ggagggccga aggcccgttt cgaaggcgca ctattttttt
     3061 ccgctagatc agcccgactt gatggaactt ggcttcgacg atgtcgcggt cgaacggctt
     3121 catgatgtat tcgttggcgc cggcattgat cgcgcgggca atatgcgcgg cgtcgttctc
     3181 ggtggtgcag aagatcacct tcggcgcgct cccgccacgc tcccggcgga gagcgacgag
     3241 aaaggcgatg ccgtccatca ccggcatatt ccagtcgagc agcaccacgt cgggcatcga
     3301 cgcgcggcac tgatcgagcg catcgcgccc gttctcggcc tccgaggccg tgaaggaaag
     3361 ctcctccaag atgcgtcgtg ccaccttgcg gatgacggcg ctgtcgtcga ccaccaagca
     3421 gtgtttcatg ccggcagcct tgctctcatc ggggtcaggc cgcgctgaac gcgacttcgc
     3481 cctcgcgcgc ctcggcggtg acggccatgc ccgaagcgac agcgacccgt ttggcgtagt
     3541 agggctgaat cgaatgggta tcgattgtca cgctatcggc ccctgacaag aggctccgca
     3601 cctgatcggc aaggcgcgcc gcatcgcccg tagccttgac aagaaagctg gccgcgggcg
     3661 gcgttccacc gatctcgaca tcgatggtgc ccccgcgcgg cagggccacg aggccgagcg
     3721 cgacgaggtt gagaagaagt ttgaccttgt tcttgggcag agtggccgcg ggcccgcgcc
     3781 aattgacctg atgctttccg gtccagcgca cgaagtcgcg ggccaccttc tcggcgtcgc
     3841 ggaggtcgat ctcggcaccc gccgatcccg cagcgccata ggcaaggcgg gcgaattgaa
     3901 gcttcgcgga cgcttgtgcc gcgctcttgc ggatgagatc catggcggct tcccgcatgg
     3961 cctcgtccgg ctcgtccccc agcatctcca gcccattggc aatggccgcc acgggattga
     4021 tcacgtcgtg gcaaatgcgg ctcgagacga gcgcagcaag ctcgagatcg ctgagtgccg
     4081 caggctcagt catatggcct ccccctcgct ttccgctcct tgcggcggag acgtggctca
     4141 cacccgtttt tctgttacat ggggacccgc gcctccacgc ccccaaaagc cgcatcgaga
     4201 ggcgcggaac gaatcaatct cgcaacggag ccattttgcc ttgttacccc cgagagagac
     4261 aacagcacgg gcgaaggcgt tagggtctct cggcttcgac ttcggccgcg tcgcgtcgcg
     4321 tctgaccgat gccgccgtgc gtgccggcgc cgccatcatg cggcattgtc acggggatcc
     4381 ggaggtccag ctcaaaggcg acgcgagtcc ggtcacgcgc gccgaccgga actcggaggc
     4441 gatcatcctg gcggcgctcg cacagtttgc ccccgaggtg aaagtcatct gcgaggaaac
     4501 cttcggcggg gcgtccgggc cgcttccgca ccgcttcttc cttgtcgatc ctctggacgg
     4561 cactaaggag ttcatccaga agcggagcga tttcaccatc aacattgccc tgatcgaggg
     4621 cgggcgtccg cgttttggtc tcgtctacgc cccggcgcgc gcgcttctcg cggtgaccat
     4681 agaagccggc aaggcggtgg aggtcgaact tcctccgaat gaggcaggcg ccgatctcga
     4741 tcgtctaaat ccgaagccga ttcatgcccg tgttgccgag aacagcggct tgaccgcgct
     4801 cgtgagccac tcccacctcg atcccgagac cgaagccttt cttgccaaac tcaacattgc
     4861 cgagcgcgtt ggcgtcggct cgtcgatcaa gtttgttgcc atcgccagag gagaggccga
     4921 cgtctatccg cgcttcggcc ccaccatgga atgggatacc gcggcgggcc aggccgtgct
     4981 ggaggcggcc ggcggtcggg tcgtcgacac aaacgacgaa ccgctccgtt atggcaaaac
     5041 cgagcagagc ttgcgcaatc cgagcttcgt cgcgtggggg cgaggcagtt cctgagcccc
     5101 atcgcgggtc gcgggcgcgc gtcgggccca cgcggggaac caagccacgc tttggtaacg
     5161 gtttaagcaa attataggga tcgcgagggg cggcgggcgg gcgcgatccg gtgtcgcgcc
     5221 gcgcgtgttc ggacccggag ccatggtcat gcatctcaga gtttccgctt ctgcagtcct
     5281 gattgccctc gtgtccgtcc tcgccccggc ggcggctcaa gatcgcggcg agtcaggatc
     5341 gggcattcgc ctggcacagg ccgaaggccc catggtgatt acgccgcagc cttattccgg
     5401 ccagcccggt tcctatccgc ccccgcagca atattccgac cagcccggtt cctatccgcc
     5461 tccgcagccc tattccggcc agcccggttc ctatccgccc ccgcagccct attccggcca
     5521 gcccggttcc tacccgcctc ccccgaacgg ttatgacgca ggcggtcctg gggctcctta
     5581 cgagccggcg ccgcaaggtg gagcctatgt gccgaggagc ggcggctaca gctcgcaagc
     5641 agccggagac g
//
LOCUS       DASUQP010000147        16975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_74778,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000147 DASUQP010000000
VERSION     DASUQP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 16975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16975
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74778"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..375
                     /locus_tag="VFY74_06530"
     tRNA            291..375
                     /locus_tag="VFY74_06530"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:325..327,aa:Tyr,seq:gta)
     gene            404..477
                     /locus_tag="VFY74_06535"
     tRNA            404..477
                     /locus_tag="VFY74_06535"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:436..438,aa:Gly,seq:tcc)
     gene            611..687
                     /locus_tag="VFY74_06540"
     tRNA            611..687
                     /locus_tag="VFY74_06540"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            752..949
                     /gene="secE"
                     /locus_tag="VFY74_06545"
     CDS             752..949
                     /gene="secE"
                     /locus_tag="VFY74_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX5931391.1"
                     /translation="MARTNPFEFLQQVRTEVSKVTWPTRRETVITTAMVLLMSVAAAL
                     FFLAADWVLSTVVTFVLGLGR"
     gene            967..1497
                     /gene="nusG"
                     /locus_tag="VFY74_06550"
     CDS             967..1497
                     /gene="nusG"
                     /locus_tag="VFY74_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX5931392.1"
                     /translation="MAMRWYIVHAYSNFERKVAESIKERAVAAGLSDMFEEVLVPMEE
                     VVEMRRGRKVSSERKFFPGYVLVKMELNDQTYHLIKSTPKVTGFLGTENKPIPITEEE
                     AGRILQQVQEGVERPKPSVTFEIGEQVRVADGPFASFNGLVEEVDEERARLKVAVSIF
                     GRPTPVELEYAQVEKL"
     gene            1533..1958
                     /gene="rplK"
                     /locus_tag="VFY74_06555"
     CDS             1533..1958
                     /gene="rplK"
                     /locus_tag="VFY74_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX5931393.1"
                     /translation="MKKIEGYIKLQVPAGQANPSPPIGPALGQRGVNIPEFCKQFNAA
                     TQKLEPGSPIPTVITVFADRSFTFEMRTPPASYLLKKAAKIGKGSKEPGRAGAGTVTQ
                     AQLREIAEVKMKDLNASSIEQAMKIIAGSARSMGLQVQE"
     gene            1962..2669
                     /gene="rplA"
                     /locus_tag="VFY74_06560"
     CDS             1962..2669
                     /gene="rplA"
                     /locus_tag="VFY74_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5931394.1"
                     /translation="MSKPGKRFREAAAKVNREKLYSLEDAVKLVKGGAKAKFDETIEV
                     AMNLGVDPRHADQMVRGVCQLPHGSGRKLRVGVFAKGAKAEEAKKAGADVVGAEDLVE
                     QVQKGVIDFDRCIATPDMMPLVGRLGKVLGPRGLMPNPKVGTVTTDIAPAVRAAKGGA
                     VEFRVEKAGLIHAGVGKASFSEQALVENIRAFADAVMKAKPSGAKGTFLKRVAVTSTM
                     GPGVKIEPASLAGGAAG"
     gene            3012..3527
                     /gene="rplJ"
                     /locus_tag="VFY74_06565"
     CDS             3012..3527
                     /gene="rplJ"
                     /locus_tag="VFY74_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5931395.1"
                     /translation="MERAEKREVVTALHDVFAKTGVVVVAHYAGLTVAQMTRLRSEMR
                     NAGGRVKVAKNRLVKLALEGTDAKGIADLLKGPTCLAFSADPIAAPKIAVKFAKANDK
                     FVILGGTMGATVLDAKGVTSLADLPSLDELRGRLIGLIQAPASKIARTLNEPGAQLAR
                     VFGAYGKSQAA"
     gene            3577..3954
                     /gene="rplL"
                     /locus_tag="VFY74_06570"
     CDS             3577..3954
                     /gene="rplL"
                     /locus_tag="VFY74_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX5931396.1"
                     /translation="MADLQKIVDTLSGLTVLEAAELAKLLEEKWGVSAAAPVAMMAAG
                     GGAAAAAPAEEQTEFTVVLAAAGAQKINVIKEVRVITSLGLKEAKDLVEGAPKPVKEG
                     VSKDEAEKIKKQLEGAGATVELK"
     gene            4112..8248
                     /gene="rpoB"
                     /locus_tag="VFY74_06575"
     CDS             4112..8248
                     /gene="rpoB"
                     /locus_tag="VFY74_06575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5931397.1"
                     /translation="MAQQTSSGPKRLRKMFGKIHEVAEMPNLIEVQKQSYDQFLMVEE
                     PEGGRGDEGLQGVFRSVFPIKDFGERSQLEFVRYAFEPPKYDVDECQQRGMTYAAPLK
                     VTLRLIVFDVNEETGARSVKDIKEQDVYMGDMPLMTSNGTFVINGTERVIVSQMHRSP
                     GVFFDHDRGKTHSSGKLLFAARIIPYRGSWLDFEFDAKDIVFVRIDRRRKLPVTTLLY
                     ALGLEDEEILSTFYDRIALKATKEGWRMPFRSDRLRGIKPTSDLIDAKTGRVAIEAGR
                     KVTARLAKKLADEGLKELLVSDEDLHGRYLAQDIVNMETGEIYGEAGEELDAKLLETL
                     KGLGLKEIPILDIDHVNIGAFIRNTLAIDKNSNPEEALLDIYRVIRPGEPPTPEAAAT
                     LFNGIFFDSERYDLSAVGRVKMNMRLDLDAPDTMRTLRKEDILAVIKTLVDLRDGKGE
                     IDDIDHLGNRRVRSVGELMENQYRVGLLRMERAIKERMSSVDIDTVMPQDLINAKPAA
                     AAVREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGR
                     ICPIETPEGPNIGLINSLATYARVNKYGFIESPYRRVVKAKVTDEVVYLSAMEEARYI
                     IAQANAKLAPNGTFIEDLIVCRHAGDVELMPPEKVDLIDVSPKQLVSVAAALIPFLEN
                     DDANRALMGSNMQRQAVPLITAEAPLVGTGMEEVVARDSGAAIGARRTGVVDQVDATR
                     IVVRATEETDPSKSGVDIYNLRKFQRSNQNTCINQRPRVRVGDHVQAGEIIADGPSTD
                     LGELALGRNVLVAFMPWMGYNFEDSILISERVVRDDVFTSIHIEEFEVMARDTKLGPE
                     EITRDIPNVSEEALKNLDEAGIVYIGAEVNPGDILVGKITPKGESPMTPEEKLLRAIF
                     GEKASDVRDTSLKLPPGVSGTVVEVRVFNRHGVEKDERAMAIEREEIERLAKDRDDEL
                     AILDRNVYGRLKDVLMHKVVAKGPKGIKKDAKITEALLEDVARSHWWEIALKNEKAMA
                     EVEAIRRQYEEAKKRLEQRFVDKVDKLQRGDELPPGVMKMVKVFVAVKRKIQPGDKMA
                     GRHGNKGVISQIVPIEDMPYLEDGTSIDIVLNPLGVPSRMNVGQILETHLGWACRGLG
                     RKIGEALEAYYQSHKAKPLKEMLKTIYGDDKAVAALKEDQVIEASESLKTGIPVATPV
                     FDGAHEGDVTQLLEAAGLDSSGQVTLYDGRTGEAFERKVTVGYIYMLKLHHLVDDKIH
                     ARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVAGRT
                     KVYEAIVRGDDTFEAGIPESFNVLVKEMRALGLNVELVQPRPDEQPARARLPRPEAAE
                     "
     gene            8330..12523
                     /gene="rpoC"
                     /locus_tag="VFY74_06580"
     CDS             8330..12523
                     /gene="rpoC"
                     /locus_tag="VFY74_06580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5931398.1"
                     /translation="MNQEIVNLFSTQTQQQNFDQIRIGIASPEKILSWSYGEIKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRMKYKGVICEKCGVEVTLAKVRRER
                     MGHIELAAPVAHIWFLKSLPSRIGLLIDMTLKDLERVLYFEHYIVLEPGLTALKPLQL
                     LSEEDYLKAQEEYGEDSFTAAIGAEAIRELLKGLDLEKIAADLRVEIAESTSELKPKK
                     LAKRLKVVEAFIQSGNRPEWMILTVVPVIPPELRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLMELRAPDIIIRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLADMLK
                     GKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYARLDAKGH
                     AATVKQAKKLVEKEKPEVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNILHPANGSPIIVPSQDI
                     VLGLYYLSLAMDGEPGEGMMFGNVAEIHHALDAKALTLHANIKGRFVTVDAKGKPVSR
                     IYDTTPGRMLLGELLPKHTEVPFELVNRLLTKKDISNMIDVVYRHCGQKETVIFCDRI
                     MGLGFVYACQAGISFGKDDMVIPESKDAIVQKTSDLVREYEQQYNDGLITRGEKYNKV
                     VDTWTECTEQVAKEMMKRISAVVRDPKTGREKPINSIYMMSHSGARGSPAQMKQLAGM
                     RGLMTKPSGEIIETPIISNFKEGLSVLEYFNSTHGARKGLADTALKTANSGYLTRRLV
                     DVAQDCIVTEEDCGTKEGITARAILEAGEVTVSLGQRVLGRTTAETVKNPSTGKVVIK
                     AGELIEEEEVDALEEARVQEVRIRSVLTCETRNGVCAKCYGRDLARGTPVNLGEAVGV
                     IAAQSIGEPGTQLTMRTFHLGGVASAKVVDQSSLESNFDGTVKIKQRNVVTDSQGRLM
                     VMGRNTALVVLDEDGTERAVHRLLYGTHLRVDDGVRVKRGDRLAEWDPYTRPVLTEME
                     GTVDFEDLVEGVSVSERRDESTGITNSVIIDWRASPRGADLRPAMVIKDKKGKVAKLS
                     RGAEARYLLPVDAILSVEPGHQVMAGDVLARIPTESAKTRDITGGLPRVAELFEARRP
                     KDHAIIAEVSGTVQLGRDYKNKRRITIVPEGGSQEPVEYLIPKGRHLAVQDGDRIEKG
                     EFLLDGHPAPHDILAIKGVEELANYLVNEIQEVYRLQGVTINDKHIEVIVRQMLQKIE
                     VNDPGTSEFLKGEQVDRIDFEDANAAVEAAGGKPATGQPVLLGITKASLQTRSFISAA
                     SFQETTRVLTDAAVNGKADTLEGLKENVIVGRLIPAGTGGTLSRLNRIAAHRDELILE
                     ERNRVSEAQIEEAAEQDAS"
     gene            12713..13555
                     /locus_tag="VFY74_06585"
     CDS             12713..13555
                     /locus_tag="VFY74_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931399.1"
                     /translation="MPSALWAWATVGFVFCGSKDELVAVLPGLRPRYSDPRLSPYDAL
                     SSVGKDEAADPYSFGQSLVRRIKQYDRQELGTHTLSHFYCLEEGGTPDAFAADLDAAK
                     AMAATSGVTLKSIVFPRNQICRDHLRVARARGLIAFRGNPRVWFHRACPEAEQTIPKR
                     ALRLADTYVPAAGHHVHEPEEAEGMVDVPASRFLRPVRGVWYERFQFDRIVGEMTAAA
                     KARKLFHLWWHPHNFGADPDANLSFLKRILDVYASLQDRFGMRSLNMSEVAEGVLRCA
                     KPSS"
     gene            13649..14293
                     /locus_tag="VFY74_06590"
     CDS             13649..14293
                     /locus_tag="VFY74_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HEX5931400.1"
                     /translation="MALLKGRAKRLGPAAAAGQVAFMAFQRLQRRYSSGRIEAIKREF
                     RLDDGPPQLGVERVDDVNGEACRMLLRERKPGVVLVMGTRLIAQATLLAAEAPFVNYH
                     AGITPKYRGVHGAYWARATCDAANCGVTVHVIDAGIDTGAILYQERISPGPADNFSTY
                     PYLQLAAGLPLLARAGKDALEGDLAPLTVDLPSKLWSHPTLWGYFAAGFGRGVW"
     gene            14618..14989
                     /gene="rpsL"
                     /locus_tag="VFY74_06595"
     CDS             14618..14989
                     /gene="rpsL"
                     /locus_tag="VFY74_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX5931401.1"
                     /translation="MPTIQQLIRKPRRMPVKRNKVPAMQSCPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNQLEVTSYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGVL
                     DTQGVGDRRQRRSKYGAKRPK"
     gene            15028..15498
                     /gene="rpsG"
                     /locus_tag="VFY74_06600"
     CDS             15028..15498
                     /gene="rpsG"
                     /locus_tag="VFY74_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX5931402.1"
                     /translation="MSRRRRADKRDVIPDPKFGDIVITKFMNSIMKEGKKSAAERIVY
                     GALDQMEKKAKQNPVELFHEALRNVMPALEVRSRRVGGATYQVPVEVRTDRRQALAIR
                     WIIAAARDRNENTMVERLSGELLDAANNRGTAVKKREDTHRMADANRAFSHYRW"
     gene            15510..>16975
                     /gene="fusA"
                     /locus_tag="VFY74_06605"
     CDS             15510..>16975
                     /gene="fusA"
                     /locus_tag="VFY74_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5931403.1"
                     /translation="MPRATPLEDYRNIGIMAHIDAGKTTTTERILYYTGKSHKLGEVH
                     DGAATMDWMEQEQERGITITSAATTAYWNDKRINIIDTPGHVDFTIEVERSLRVLDGA
                     VALLDANQGVEPQTETVWRQADKYGVPRMIFVNKMDKIGADFFVSVQMVKERLGTNPI
                     VLQLPLGAEASFAGIIDLIRMKAVKWDDESLGAKFHDEDIPASLLDQAKKYREMLVEQ
                     AVELDEAATEAYLEGKEPAEPELKALIRKGTVSSHFVPILCGSAFKNKGVQPLLDAVV
                     DFLPSPLDVPPVTGIDVKTGEEGTREPRDDAPLSMLAFKIMNDPFVGSLTFARIYSGK
                     VESGSTLLNTVKDKKERIGRMLLMHANHREDIKEAFTGDIVALAGLKDVTTGDTLSDP
                     AKPIVLERMEFPEPVIEVAVEPKTKGDQEKLGVALNRLAQEDPSFRVSVDHESGQTII
                     KGMGELHLDIIVERMRREFKVEANVGAPQVAFRETVSK"
BASE COUNT         3373 a         5225 c         5571 g         2806 t
ORIGIN      
        1 aacagtctca cccgctcggt gccgtcgcgg cggggcgctt tgccaggagt ccaagcggcc
       61 tgtcttgccg gtttattggg cctcgacctc tgccgccgca tcgtgagccg tgacgtccta
      121 gagtttgggg tctgaacccc cggggaaggg gaaattcgcc ctaaatttct gatttacagt
      181 tgacagcgcc ggacgcgcta cccataaacc cgtcaatggc ggaccctgcc ggccgacggc
      241 ggggcgtttt tgcttggtta aaatcaccag tcaatccatc ttcgcttcaa ggaggggtgc
      301 ccgagtggct aaaggggacg ggctgtaaac ccgttggcta tgcctacgta ggttcgaatc
      361 ctaccccctc caccaaccac tctttgacga gtcgagatgg aatgcgggtg tagctcaatg
      421 gtagagcagc agccttccaa gctgaggacg agggttcgat tcccttcacc cgctccaggg
      481 aacagtaatc agagatcaga aatcagatcg caaagcagga aaaaggcgag gacgcgcaac
      541 cgaaacgggc acagacgtca gcattgatga gagcttcttg agttctgatt tcgatttctg
      601 atccctgaat aggagtgtag ctcaactggc tagagcaccg gtctccaaaa ccgggggttg
      661 ggggttcgag tccctccact cctgccatga attggggcgc gagctggaag gcatgaggcg
      721 cggcgcggag cttccgcggc aaggattaag gatggcgagg actaatccat tcgagtttct
      781 gcagcaggtg cgaaccgagg tctccaaggt cacctggccg acgcgccggg agaccgtgat
      841 caccacggcg atggtgctct tgatgtcggt cgccgccgcg ctgttctttc tcgccgccga
      901 ttgggtcttg agcacggtcg tcacgttcgt cctgggtttg ggccggtagg ggtcaaagtt
      961 cgtcacatgg caatgcgctg gtacatcgtt cacgcctatt cgaatttcga gcgcaaggtc
     1021 gccgagtcga tcaaggagcg tgccgtagcg gccgggcttt ccgacatgtt cgaggaggtg
     1081 ctggtgccga tggaggaggt cgtggagatg cgccgcggcc ggaaggtcag ctccgaacgg
     1141 aagttctttc ccggctacgt gctcgtcaag atggagctca acgatcagac ctatcacctc
     1201 atcaagtcga cgccgaaagt gacaggcttt ctcggcaccg agaacaagcc cattcccatc
     1261 accgaggagg aggccgggcg catcctccag caggtgcagg agggcgtgga gcggccgaag
     1321 ccgtccgtca ccttcgagat cggcgagcag gtgcgcgtgg ccgacggccc gttcgcctcg
     1381 ttcaacggac tggtcgagga ggtcgacgag gagcgggcgc ggctgaaagt ggcggtgtcg
     1441 attttcggcc ggccgactcc ggtcgagctc gaatatgcgc aggtcgagaa gctctaggcg
     1501 ggtcaggcgg acgacgcggg aagagtcgag gaatgaagaa gatcgaagga tacatcaagc
     1561 tgcaggtgcc ggcagggcag gccaatccct cgccgccgat cgggcccgcg ctcggccagc
     1621 gcggcgtcaa catcccggag ttctgcaagc agttcaacgc ggcgacccag aagctcgagc
     1681 ccggctcgcc gatcccgacg gtgatcaccg tctttgccga ccggtccttc accttcgaga
     1741 tgcggacccc gccggcgtct tacctcctga agaaggccgc gaagatcggc aaaggatcga
     1801 aggaaccggg ccgcgccggc gcaggcacgg tcacgcaggc gcagttgcgc gagatcgccg
     1861 aggtcaagat gaaggatttg aacgcgagct cgatcgagca ggcgatgaag atcatcgctg
     1921 gctccgcccg ctcgatgggc ctacaggtgc aggagtaagt catgagcaag cccggcaaac
     1981 ggttccgcga ggcggcggcg aaggtgaacc gcgagaagct ctattcgctc gaggacgcgg
     2041 tgaagctcgt caaaggcggg gccaaggcga agttcgacga gacgatcgag gtggcgatga
     2101 atctcggcgt cgatccgcgt catgccgacc agatggtgcg cggagtatgc cagttgccgc
     2161 atggttcggg gcgcaagctc agagtgggcg tcttcgccaa gggcgcaaag gccgaggaag
     2221 cgaagaaggc gggcgccgac gtcgttggcg cggaggatct cgtcgagcag gtgcagaagg
     2281 gcgtgatcga tttcgaccgc tgcatcgcca cgcccgacat gatgccgctc gtcgggcgtc
     2341 tcgggaaggt gctcggccct cgcgggctga tgcccaatcc caaggtcggg acggtcacga
     2401 ccgatatcgc cccggccgtg cgcgcggcaa agggcggcgc cgtcgaattc cgggtcgaga
     2461 aggccggcct catccatgcc ggcgtcggca aagcgagctt cagcgagcag gcgcttgtcg
     2521 agaatatccg cgccttcgcc gatgcggtga tgaaggccaa gccctccggc gccaagggca
     2581 cgttcttgaa gcgggtcgcg gtcacttcga ccatgggccc gggcgtgaag atcgagcctg
     2641 caagcctcgc cggaggcgct gcgggctaga gtagagtttc ggggccgagc gatcggctcc
     2701 gaaggatcgc ttgctagccg caagcggtcc gaacctgtcc gagattgcag gtgcgggcga
     2761 ccgcttaagc ctttcgggcc tgcatgagac gggagacgaa cggttcggag gcggcgggtc
     2821 tgcccgcaac ctcaagatcg gtttgaacct cgtaaggagc tcttgcgccg tttggcgtgc
     2881 gggcggacag gctagagaag cgccgcttga agacgcgggc aggctcttag ccttgccctc
     2941 ttaattctcg cggcacggtg tggccgaaag ggcagggcct tttgcctcgc ccgcaacaga
     3001 cggagtgagc attggagaga gcagagaagc gcgaagtcgt gacggcgctt cacgatgtct
     3061 tcgccaagac cggcgtcgtc gtcgtcgctc actatgcggg cctgaccgtc gcccagatga
     3121 ccagattgcg cagcgagatg agaaacgctg gcggccgggt caaagtggcg aagaaccggc
     3181 tcgtcaaact cgcgctcgaa ggcaccgacg ctaagggcat cgcggatctg ctcaaggggc
     3241 cgacgtgcct cgcattttcc gcggacccga tcgcggcacc gaagatcgcc gtcaaattcg
     3301 ccaaggcgaa cgacaaattc gtgatcctcg gcggcacgat gggcgccacg gtgctcgatg
     3361 ccaagggcgt gacttcgctt gccgacttgc cttcgctcga cgagctcaga ggcaggctga
     3421 tcggtcttat tcaggcgccc gcgagcaaga tcgcgcgcac cctgaacgag cccggcgccc
     3481 agcttgcacg cgtgttcggc gcttacggca agtcgcaggc ggcgtgacgg cggcctgaaa
     3541 cctttacgag agagtcaaac ctacaaggat aagtcgatgg cagatttgca gaaaatcgtg
     3601 gatacgcttt cgggcctgac cgtgctcgaa gccgcagagc ttgcgaaatt gctcgaagag
     3661 aaatggggcg tctcggctgc cgctccggtc gccatgatgg ccgccggcgg gggggccgcg
     3721 gccgcggccc cggccgagga gcagacggag ttcaccgtcg tgctggcggc cgccggagcc
     3781 cagaagatca atgttatcaa agaggtaagg gtgatcacca gtctcggcct gaaagaggcc
     3841 aaagacttgg tcgaaggtgc gcccaagccc gtgaaggaag gcgtttccaa ggacgaggcg
     3901 gagaaaatca agaagcagct cgaaggcgcc ggcgcgactg tcgagctgaa atagtggtgg
     3961 gttgaaagct gcccgggcgg gcacaatata taggtgttcg tccgggcaag aacccaccaa
     4021 aacaacgcgt gacctgacgt ttcgccagac gctccgcttt catcgaagcg gggagtccgg
     4081 cgagccgtcg cgacgagcct aagaggaccc aatggcgcag cagacttcga gtggccctaa
     4141 gcgtttgcgg aagatgttcg gcaagatcca cgaagtggcc gagatgccga acctcatcga
     4201 ggttcagaag cagtcctacg atcaatttct catggtggaa gagcccgagg gcggccgcgg
     4261 ggacgaaggc ttgcagggcg tgttccgctc cgtgttcccc atcaaggatt tcggcgagcg
     4321 ctcgcagctc gaattcgtgc gctatgcgtt cgagccgccg aaatacgacg tcgatgagtg
     4381 ccagcagcgg ggcatgacct atgccgcgcc gctcaaagtg acgctcaggc tgatcgtgtt
     4441 cgacgtcaac gaagagacgg gcgcgcgctc ggtcaaggac atcaaggaac aggacgtcta
     4501 catgggcgac atgccgctca tgacgtcgaa cggcaccttt gtcatcaacg gcaccgagcg
     4561 cgtgatcgtc tcgcaaatgc accgctcgcc cggcgtcttc ttcgaccacg accggggcaa
     4621 gacccattcc tcgggcaagc ttctgttcgc ggcgcgcatc attccctatc gcggctcgtg
     4681 gctcgatttc gagttcgacg ccaaggatat cgtctttgtc cgcatcgacc ggcgccgcaa
     4741 gctgccggtg acgaccctgc tctatgcgct cggcctcgag gacgaggaga ttctgtccac
     4801 gttctacgac cgcatcgcgc tgaaggcgac caaggaggga tggcgcatgc cgttccgctc
     4861 cgaccgtttg cgcgggatca agccgaccag cgacctgatc gacgccaaga ccggcagggt
     4921 cgcgatcgaa gccggccgca aggtcaccgc gcgtctcgcc aagaaactcg ccgatgaggg
     4981 cctgaaggag ctactcgtga gcgacgagga cctccacggc cgctacctcg cgcaggacat
     5041 cgtcaacatg gagacgggcg agatctacgg cgaggccggc gaagagctcg acgccaagct
     5101 gctcgagacg ctcaagggtc tcggtctcaa ggagatcccg atcctcgaca tcgaccatgt
     5161 caatatcggc gcgttcatcc gcaacacgct cgccatcgac aagaactcga atcccgagga
     5221 ggcgctgctc gacatatacc gcgtgattcg gccgggcgag ccgccgacgc ctgaagccgc
     5281 ggcaacgctg ttcaacggca tcttcttcga ttcggagcgc tacgacctgt ccgccgtcgg
     5341 tcgcgtcaag atgaacatgc gtctcgacct cgatgcgccc gacacgatgc gcacgctgcg
     5401 caaggaggac atcctcgccg tgatcaagac gctggtcgat ctccgcgacg gcaagggcga
     5461 gatcgacgat atcgaccatc tcggcaatcg ccgggtgcgt tcggtcggcg agttgatgga
     5521 gaatcagtac cgcgtcggcc ttctgcgcat ggagcgcgcg atcaaggagc gcatgagttc
     5581 ggtcgatatc gacacggtca tgccgcagga tctgatcaac gccaagcccg ccgccgcggc
     5641 cgtgcgcgag ttcttcggct cgtcccagct ctcccagttc atggaccaga ccaatccgct
     5701 gtcggagatc acccacaagc ggcgcctgtc ggcgctcgga ccgggcgggc tgacgcgcga
     5761 gcgcgcgggc ttcgaggtgc gcgacgtgca tccgacccat tacggccgca tctgcccgat
     5821 cgagacgccg gagggaccga atatcggcct catcaactcg ctcgccacgt atgcacgggt
     5881 gaacaagtac ggcttcatcg agagccctta tcgccgcgtg gtcaaagcta aggtcaccga
     5941 cgaggtggtc tatctctcgg ccatggagga ggcgcgctac atcatcgcgc aggctaacgc
     6001 caagcttgcg ccgaacggca ccttcatcga ggacctgatc gtctgccgcc atgccggcga
     6061 cgtcgagctc atgcccccgg agaaagtgga cctcatcgac gtgtcgccga agcagctcgt
     6121 gtcggtggcg gccgcgctca ttccgttcct cgagaatgac gacgcgaacc gcgcgctgat
     6181 gggctcgaac atgcagcgcc aggcggtgcc gctcatcacc gccgaagcgc cgctcgtcgg
     6241 caccggcatg gaagaggtcg tggcgcgcga ttccggcgcc gccatcggcg cccgccgcac
     6301 cggggtggtc gaccaggtgg acgcgacccg tatcgtcgtc cgcgccaccg aggagaccga
     6361 tccgtcgaag tcgggcgtgg acatctataa tttgcgcaag ttccagcgct cgaaccagaa
     6421 cacctgcatc aatcaacggc cgcgggtgcg cgtgggcgat cacgtccagg cgggcgagat
     6481 catcgccgac ggtccgtcga ccgatctcgg cgagctggcg cttggccgga acgtgctcgt
     6541 cgccttcatg ccgtggatgg gctacaactt cgaggactcc atcctcatct ccgagcgcgt
     6601 cgtccgcgac gacgtgttca cctcgatcca tatcgaggaa ttcgaggtga tggcgcgcga
     6661 caccaagctc gggccggagg aaatcacccg cgacattccc aacgtctcgg aagaggcgct
     6721 caagaatctg gacgaagcgg gcatcgtcta tatcggtgcc gaggtcaatc cgggcgacat
     6781 tctcgtgggc aagatcacgc ccaagggcga aagcccgatg acgccggagg agaagctgct
     6841 ccgcgccatt ttcggcgaga aggcctccga cgtgcgcgac acctcgctca aattgccgcc
     6901 cggcgtctcc ggcaccgtgg ttgaagtgcg cgtgttcaac cgacacggcg tcgagaagga
     6961 cgagcgcgcc atggccatcg agcgcgagga gatcgagcgg ctcgccaagg accgcgacga
     7021 cgagctcgcg atcctcgacc gcaacgtcta tggccgcctc aaggacgtgc tgatgcacaa
     7081 ggtcgtggca aaaggaccga agggcatcaa gaaagacgcc aagatcaccg aggctctgct
     7141 cgaggacgtt gcgcgcagcc attggtggga gatcgccctc aagaacgaga aggccatggc
     7201 cgaggtcgag gcgatccgcc gccaatacga agaggccaag aagaggctcg agcagcgctt
     7261 cgtcgacaag gtcgacaagc tccagcgcgg cgatgagttg ccgccgggcg tgatgaagat
     7321 ggtcaaggtc ttcgtggcgg tgaagcgcaa gattcagccc ggcgacaaga tggccggccg
     7381 ccatggcaac aagggcgtga tcagccagat cgtgccgatc gaggacatgc cctatctgga
     7441 ggacggcacc tcgatcgaca tcgtgctcaa tccgctcggc gtgccgagcc gcatgaatgt
     7501 gggccagatc cttgagaccc atctcggctg ggcttgccgg gggctgggcc gcaagatcgg
     7561 cgaggcgctc gaagcctact atcaaagcca taaggcaaag ccgctcaagg agatgctgaa
     7621 gacgatctac ggcgacgaca aggccgtcgc ggcgctcaag gaggatcaag tcatcgaggc
     7681 gagcgagagc ctgaagaccg ggattccggt ggcgacgccc gtgttcgacg gcgcgcatga
     7741 aggcgacgtc acgcaactcc tcgaagccgc gggcctcgac agctcgggac aggtcacgct
     7801 ctatgacggg cgcaccggcg aggcgttcga gcgcaaggtg acggtgggct acatctatat
     7861 gttgaagctc caccacctcg tcgacgacaa gattcatgcc cgctcgatcg gaccctatag
     7921 cctcgtgacc cagcagccgc tcggcggcaa ggcgcaattt ggcggccagc gtttcggcga
     7981 gatggaggtg tgggcgctcg aagcgtatgg cgccgcctac acgctgcagg agatgctcac
     8041 cgtcaaatcc gacgacgtgg cgggccgcac caaagtttac gaagccatcg tacgcggcga
     8101 cgacacattc gaggccggca tccccgagtc gttcaacgtt ctcgttaagg agatgagggc
     8161 gctgggcttg aacgtcgagc tggttcagcc gcggcctgac gaacagccgg cccgggcgcg
     8221 actgccgcgc ccggaggcgg ccgagtgatt gcggccccgc gtggggccat tgaccaacga
     8281 cgacgaccaa ccgacaggcg gcaaggccga ccggtaggag accaagagaa tgaatcagga
     8341 aatcgtcaat ctcttcagca cgcagaccca gcagcagaat ttcgatcaga tccggatcgg
     8401 catcgcgagc ccggaaaaga ttctgtcgtg gtcgtatggg gagatcaaga agcccgagac
     8461 aatcaactac cgcaccttca agccggagcg tgacgggctc ttctgcgcgc gcatcttcgg
     8521 cccggtgaag gactacgagt gcttgtgcgg caagtacaag cgcatgaagt acaagggcgt
     8581 catctgcgag aaatgcggcg tcgaggtgac gctcgccaag gtccggcgcg agcgcatggg
     8641 ccatatcgag ctcgccgcac cggtcgcgca tatctggttc ctgaagtcgc tgccgtcgcg
     8701 catcgggctc ctgatagaca tgacgctcaa ggaccttgag cgggtgctct atttcgagca
     8761 ctacatcgtg ctcgagccgg gtctgaccgc gctgaagccg cttcagctct tgagcgagga
     8821 ggactatctc aaggcgcaag aagagtacgg cgaggattct ttcaccgccg cgatcggcgc
     8881 cgaagccatc cgcgagctgc tgaagggcct cgatctcgag aagatcgccg ccgatctccg
     8941 cgtcgagatc gccgagtcga cctccgagct caagccgaag aagctggcga agcgcttgaa
     9001 ggtggtcgag gccttcatcc agtcgggcaa ccggcccgag tggatgatcc tgaccgtggt
     9061 tccggtcatt ccgccggagc tgcggccgct ggtgccgctc gacggcgggc gcttcgccac
     9121 ctcggacctg aacgatctct accgtcgcgt catcaaccgc aacaaccgtt tgaagcggct
     9181 gatggagctg cgtgcgccgg acatcatcat ccgcaacgag aagcgcatgc tgcaggaagc
     9241 ggtcgatgcg ctgttcgaca acggccggcg cggccgcgtc atcaccggcg cgaacaagcg
     9301 gccgctgaag tcgctcgccg acatgctcaa gggcaagcag gggcgcttcc gccagaacct
     9361 gctcggcaag cgcgtcgact attcgggccg ttcggtgatc gtggtcgggc cagagctcaa
     9421 gctgcatcaa tgcggcctgc ccaagaagat ggcgctcgag ctgttcaagc cgttcatcta
     9481 tgcgcggctc gacgccaagg gccatgccgc gacggtgaaa caggcaaaga agctggtcga
     9541 gaaggagaag ccggaggtct gggacatcct cgatgaggtg atccgcgagc atccggtgct
     9601 actcaaccgc gcgcctacgc tccaccgcct cggcatccag gcgttcgagc cggtgctgat
     9661 cgagggcaag gcgatccagc ttcacccgct cgtctgcgcc gccttcaacg ccgatttcga
     9721 cggcgaccag atggcggttc acgtgccgct gtcgctcgag gcgcagctcg aggcccgcgt
     9781 gctgatgatg tcgaccaaca acatcctgca tccggccaat ggctcgccga tcatcgtgcc
     9841 gagccaggac atcgtgctcg ggctctatta cttgtcgctg gccatggacg gagagcctgg
     9901 cgaggggatg atgttcggca acgtggccga gatccatcat gcgctcgacg ccaaggccct
     9961 gaccttgcac gccaacatca agggccgttt cgtcacggtc gacgccaagg gcaagccggt
    10021 gtcgcggatc tacgacacga ccccggggcg catgctcctc ggcgagcttc tgccgaagca
    10081 cacggaggtg cccttcgaac tcgtcaaccg gctgctcacc aagaaggaca tctccaacat
    10141 gatcgacgtg gtctatcgcc actgcggtca gaaggagacg gtcatcttct gcgacaggat
    10201 catggggctg ggcttcgtct atgcctgcca ggccggaatt tcattcggca aggacgacat
    10261 ggtgatcccg gagagcaagg acgccatcgt gcagaagacg tccgatctcg tgcgcgagta
    10321 cgagcagcaa tataacgacg gcctgatcac gcgcggcgag aaatacaaca aggtggtcga
    10381 cacctggacg gagtgcaccg agcaggtcgc caaggagatg atgaagcgca tctcggcggt
    10441 cgtgcgcgat ccgaagaccg gccgggagaa gccgatcaac tccatctata tgatgtcgca
    10501 ttcgggcgcg cgcggttcgc cggcccagat gaagcagctt gccggcatgc gcggcctgat
    10561 gaccaagccg tcgggcgaga tcatcgagac gccgatcatc tccaacttca aggaggggct
    10621 gtcggtgctc gaatatttca actcgaccca cggcgcgcgg aaaggtcttg ccgatacggc
    10681 gctaaaaacc gcgaactccg gctatctcac ccggcgtctc gtcgacgtgg cgcaggactg
    10741 catcgtcacc gaagaggact gcggcaccaa ggaaggcatc accgcgcgcg ccattctcga
    10801 ggcgggcgag gttaccgtgt cgctcggaca gcgcgtgctc ggccgcacca cggccgagac
    10861 cgtcaagaat ccttcgaccg gcaaggtggt gatcaaggcc ggcgagctga tcgaggaaga
    10921 agaggtcgat gcgctggagg aggcgcgcgt gcaggaggtc cgcatccgct cggtgctgac
    10981 ctgcgaaacc cgcaacggcg tttgcgccaa gtgctacggg cgcgatctcg cccgcggaac
    11041 gccggtcaat ctcggcgagg ccgtcggcgt catcgccgcg cagtcgatcg gcgaacccgg
    11101 cacgcagctc accatgcgga ccttccacct tggcggcgtg gcgtcggcca aggtcgtcga
    11161 tcagtcttcc ctggagtcca atttcgacgg tacggtgaag atcaagcagc gcaacgtggt
    11221 cacggattcg caaggccggc tcatggtcat gggacgcaac acggccctcg tcgtcctcga
    11281 cgaggacggc accgagcgtg ccgtgcatcg cctgctttac ggcacgcatc ttcgggtcga
    11341 tgacggcgtc agggtcaagc gcggcgaccg gcttgccgag tgggatccct atacccgtcc
    11401 cgtgctcacg gaaatggagg gcacggtcga cttcgaggat ctggtcgaag gcgtgtcggt
    11461 cagcgagcgg cgcgacgagt cgaccggcat caccaatagc gtgatcatcg actggcgcgc
    11521 atcgccgcgc ggtgccgatc ttcggcccgc gatggtgatc aaggacaaga agggcaaggt
    11581 cgccaagctg tcgcgcggcg ccgaggcccg ctacctgctg cccgtggatg cgatcctctc
    11641 ggtcgagccc ggccatcagg tcatggccgg cgacgtgctc gcgcgcattc cgacggagag
    11701 cgcaaagacc cgcgacatca ccggcggtct cccgcgcgtt gccgagctgt tcgaggcgcg
    11761 gcggcccaag gaccacgcca tcatcgccga ggtgtcgggc acggtgcagc taggccgcga
    11821 ctacaagaac aagcggcgca tcaccatcgt gccggagggc ggcagccagg agccggtcga
    11881 gtatctcatc ccgaagggac ggcatctcgc cgtgcaggac ggcgaccgca tcgagaaggg
    11941 cgagttcctg ctcgacggcc atcctgcgcc gcacgacatc cttgccatca agggcgtcga
    12001 ggagctggcc aactacctcg tgaacgagat ccaggaggtc taccggctgc agggcgtcac
    12061 catcaacgac aagcatatcg aggtgatcgt caggcagatg ctgcaaaaga tcgaggtcaa
    12121 cgatccgggg acaagcgagt tcctcaaggg cgagcaggtg gaccggatcg acttcgagga
    12181 cgccaatgcg gcggtcgaag cggccggcgg caagcccgcc accggacagc cggtgctgct
    12241 cggaatcacc aaggcctcgc tccagacccg ctcgttcatc tcggcggcgt cgttccagga
    12301 gacgacacgc gtgctcaccg atgccgccgt gaacggcaag gccgacacgc tcgagggcct
    12361 caaggagaac gtgatcgtcg ggcggctcat tccggccgga acgggcggca ccttgagccg
    12421 gctcaaccgg atcgcggccc atcgtgacga gctgattctc gaggaacgga accgggtctc
    12481 ggaggctcaa atcgaggagg cggccgaaca ggacgcaagc tgacgcgcga gagttccccg
    12541 gcgggatgac ccgccgggga gaccaaggat tgcagtgggg tggaattcgg cgcgctggtc
    12601 atatcgctcg atttcgagct ttattggggg gtgcgcgaca agcgctcgct cgcggcatac
    12661 gggcgcaaca tcctcggcgg ccgccgagcg attccaagga tgctcgatct gtttgccgag
    12721 cgcgctgtgg gcctgggcaa cggtcgggtt cgtgttctgc ggatccaagg acgagttggt
    12781 tgccgtgctg ccggggctaa ggccgcgcta ttccgatccg cggctgtcgc cgtatgacgc
    12841 gctttcctcc gtcggcaagg acgaagccgc cgatccctat tctttcggcc agtcgcttgt
    12901 gcggcggatc aagcagtacg accggcagga gttgggcacg cacacgctct cgcacttcta
    12961 ttgtctggag gagggaggca cgccggacgc cttcgcagca gaccttgacg cagccaaggc
    13021 gatggccgcg acgagcggtg tgacgctgaa gagcatcgtg ttcccgcgca accaaatctg
    13081 ccgcgatcat ctgcgcgtcg cacgtgcgcg ggggctcatc gcctttcgcg gcaatccgcg
    13141 cgtctggttt caccgtgcct gtcccgaggc ggagcagacc attccgaagc gcgccctgcg
    13201 gcttgccgac acttacgtgc ccgcggcagg ccaccatgtg catgagccgg aagaggcgga
    13261 aggcatggtc gacgtgccgg cgagccgctt cctgcggccc gtgcgcgggg tgtggtacga
    13321 gcggtttcag ttcgatcgca tcgtggggga gatgaccgcc gccgccaagg ccaggaaact
    13381 gttccacctt tggtggcacc cgcacaattt cggcgccgat cccgatgcca atctgtcgtt
    13441 cttgaaacgc attctcgacg tgtacgcatc gctgcaagac cggttcggca tgcggtccct
    13501 gaacatgtcg gaagtggccg aaggcgttct gcgatgcgcc aagccgtcct cctgacctgc
    13561 gagggcgagt tcggccgtat cgccgcaaaa ctatcttgcg ccacattttc cggggctcct
    13621 caccatcgtg gatggaccgc agtcgcgctt ggcgctgctg aagggccgcg ccaagcgcct
    13681 ggggcctgcg gcagccgcgg gtcaagtggc gttcatggcc ttccagcggc tccagcggcg
    13741 ctattcgagc ggccggatcg aagccatcaa gcgggaattt cggctcgacg atggacctcc
    13801 gcagctcgga gtggaacgcg tcgacgatgt gaacggcgag gcctgccgca tgctcttaag
    13861 ggaacgaaag ccgggcgtcg tgctggtcat ggggacacgg ctgatcgcgc aagcgaccct
    13921 gcttgcggcg gaggcgccct tcgtcaacta tcatgctgga attacgccca aatatcgcgg
    13981 cgtgcacggc gcctattggg cacgcgccac gtgcgatgcg gcaaattgcg gtgtcacagt
    14041 tcatgtgatc gacgcaggca tcgacacggg ggcgatcctc tatcaggaga gaatctcgcc
    14101 tgggccggcc gacaatttct cgacttatcc ctatttgcag ttggcggcag ggcttccgct
    14161 ccttgcgcgc gcgggcaagg acgcgcttga gggcgatttg gcgccgctta ccgtcgactt
    14221 gccatcgaag ctctggtcgc atccgacgct ttgggggtat ttcgccgcag ggttcggacg
    14281 cggcgtgtgg taggtcgcgg agaggagaat tggcgaaaaa ctgcgtgttt ctctattgac
    14341 gatggaagaa tcggcctgtt acaacgcgcg cactttcgcg gcaggcggtg gcccaatttt
    14401 tcaggttgcc ttcgggcgcg ggttagacgc tgccggacta accgggatta catcggggtc
    14461 atgatcttgg aggcgtaggt cctccgaggt cctctgcgct gacaggccga aggtgaaggg
    14521 acccgtcccg cacttcggct ttggattgta tgggcttttg cccacgcttt ggatgacgcg
    14581 ggctccgccc gcagggacga ttggacgaag gcttacgatg ccgacgatac aacagctgat
    14641 ccgcaagccg cggcgaatgc cggtcaagcg gaacaaggtg ccggcgatgc agtcctgccc
    14701 gcagaagcgg ggggtctgta cgcgcgtcta caccacgacg ccgaagaaac ccaactcggc
    14761 gctccgcaag gtcgcgcgcg tccgcctcac caatcagctc gaagtgacga gctacatccc
    14821 cggcgagggc cacaatctcc aggagcactc ggtggtgctg atcaggggcg ggcgcgtcaa
    14881 ggaccttccc ggcgtccgct accacatcat tcgtggcgtg ctcgacaccc aaggcgtagg
    14941 cgaccgccgg cagcggcgct cgaaatacgg agccaagcga cccaaataat gggtcgtttt
    15001 gcgttttaac aaagcggacc agacgatatg tcgaggcggc gtagggcaga taagcgcgat
    15061 gtgatccccg accccaagtt cggggacatc gtcattacca aattcatgaa cagcatcatg
    15121 aaggaaggca agaaatccgc cgccgagcgg atcgtctatg gcgcgctcga ccagatggag
    15181 aagaaggcca agcaaaaccc cgtcgagctg ttccacgagg cgcttcgcaa cgtcatgccc
    15241 gcgctcgagg taaggtcgcg gcgcgtcggc ggagccacct atcaggtccc ggtcgaggtg
    15301 cgtaccgacc ggcgccaggc tctggctatc cgctggatca tcgccgccgc ccgcgaccgc
    15361 aacgagaaca ccatggtcga gcgcctctcc ggcgagctgc tcgatgccgc caacaatcgt
    15421 gggaccgcgg tgaagaagcg cgaggatacc caccgcatgg cggatgccaa ccgcgccttc
    15481 tcgcattacc gctggtaacg gagtcgacga tgccgcgagc cacccccctc gaggactacc
    15541 gcaatatcgg aatcatggcg catatcgacg ccggcaagac cacgacgacg gagcggatcc
    15601 tctactacac cggtaagagc cacaagctcg gcgaggtgca cgacggcgcc gccaccatgg
    15661 actggatgga acaggagcag gagcgcggca tcaccatcac ttcggccgcg accaccgctt
    15721 attggaacga caagcgcatc aacatcatcg acacgccggg ccacgtcgac ttcaccatcg
    15781 aagtcgagcg gtcgttgcgc gtgctcgacg gcgccgtcgc gctgctcgac gccaatcagg
    15841 gcgttgagcc gcagacggaa actgtctggc gtcaggccga caaatacggc gtgccgcgca
    15901 tgatcttcgt caacaagatg gacaagatcg gcgccgattt cttcgtgtcc gtgcagatgg
    15961 tgaaggagcg cctcggcacg aacccgatcg tgcttcagct cccgctcggc gcggaagcga
    16021 gcttcgccgg cattatcgac ctcatccgca tgaaggcggt gaagtgggac gacgagtcgc
    16081 tgggcgcaaa gttccacgac gaggacatcc ccgccagcct cctcgatcag gccaagaaat
    16141 atcgcgagat gctcgtggag caggcggtcg agctcgacga ggccgcgacc gaggcctatc
    16201 tcgaaggcaa ggagccggcg gaacccgagc tcaaggcgct gatccgcaaa ggcacggtga
    16261 gcagccattt cgtgccgatc ctgtgcggct ccgccttcaa gaacaagggc gtgcagccgc
    16321 tccttgatgc ggtggtggat tttctgccgt cgccgctcga cgtgcccccg gtcaccggca
    16381 tcgacgtcaa gaccggcgag gaggggacac gcgagccgcg cgacgacgcg ccgctctcca
    16441 tgctcgcctt caagatcatg aacgatccgt tcgtaggttc gctcaccttc gcccgcatct
    16501 attcgggcaa ggtggagtcc ggctcgacgc tgctcaacac ggtgaaggac aagaaagagc
    16561 gcatcggccg catgctgctg atgcacgcca atcaccgcga ggacatcaag gaagcgttca
    16621 ccggcgatat cgtggcgctt gccgggctga aggacgtgac gacgggcgat acgctgtccg
    16681 atccggccaa gcccatcgtg ctcgagcgca tggagttccc cgagcccgtg atcgaggtcg
    16741 cggtcgagcc gaagaccaag ggcgaccagg aaaagctcgg cgtggcgctc aatcggctgg
    16801 cccaggaaga tccctcgttc cgcgtgtcgg tcgaccatga atccggccag accatcatca
    16861 agggcatggg cgagctgcac ctcgacatca tcgtcgaacg catgcggcgc gagttcaagg
    16921 tcgaggccaa tgtcggcgcg ccgcaagtgg cgttccgcga gaccgtgtcg aaaat
//
LOCUS       DASUQP010000148         6272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1437009, whole genome shotgun sequence.
ACCESSION   DASUQP010000148 DASUQP010000000
VERSION     DASUQP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437009"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFY74_06610"
     CDS             complement(<1..274)
                     /locus_tag="VFY74_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5931404.1"
                     /translation="MMLGAFLGASLLAAATSAPHADDKFIVVQSTTSTQNSGLFDHIL
                     PLFTKKTGIEVRVVAVGTGQALKNAENGDGDVVLVHSKPDEEKFVAD"
     gene            complement(316..1086)
                     /locus_tag="VFY74_06615"
     CDS             complement(316..1086)
                     /locus_tag="VFY74_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEX5931405.1"
                     /translation="MLDPTASPRAFTTALAGRGGLLPLELRRLTYEANGKRVIDNVDL
                     ALQQPGITVIMGPNGAGKSVLLRLMHGLIAPTRGEILWAGRRMDREIALRQAMVFQKP
                     VLLRRSAAANIRHALGVRGIDKGERAIRVDGALKLAGLEGRAAMPARVLSGGEQQRLC
                     LARALSLEPEVLFLDEPTASVDPASTLAIERLLIEAQHRGIKIIVVTHDVGQARRLAQ
                     DVVFLHHGQVVEHGLAKRFFTEPRSEAARCFLAGGLVL"
     gene            complement(1079..1786)
                     /locus_tag="VFY74_06620"
     CDS             complement(1079..1786)
                     /locus_tag="VFY74_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5931406.1"
                     /translation="MTELTEAFHTALQLIVTLDSKLLGIVLLSLKVSLTAVAIASLIG
                     FALGGALAVYRFPGRGTLAAILSALMGLPPVVAGLFVYLLLSNTGPFGVLQLLYTPTA
                     MIVAQVVIVTPIVAALTRQACEDLLEEYDEQLRSLGASSAAIVGTLLWDGRYRLVTAV
                     LAGFGRAIAEVGAVMIVGGNIDHVTRTMTTTIALETSKGNIALALALGIVLLVIAFAV
                     NAALMSVRRFAARFAYA"
     gene            1996..3765
                     /locus_tag="VFY74_06625"
     CDS             1996..3765
                     /locus_tag="VFY74_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5931407.1"
                     /translation="MTALSTGRTFGVDEKTRGNGGPDRPARHVGSRKKARAFPKGRQL
                     DIEALEEIRALLGDRPRRHDLLIEYLHLIQDRYHAISAPHLKALCEELRLPQAAAYEV
                     ATFYAHFDVLKEGETPPPPTTIRVCDSITCAIKGAEALFEALKSGTDRKDVRVLYAPC
                     MGRCDTAPVAEVGHFHVDNATPKDVMDVVTGGNREPAIPAYETLAEYRAKGGYAVLEN
                     CRAGKLSFDSIIKEMTDAGLRGLGGAGFPAGRKWQIVRSYPGPRLMTINGDEGEPGTF
                     KDRFYLERDPHQMFEGALIAAWAVEAERIYLYMRDEYPAVLHILTHEIAALEEAGIIK
                     KGDIELRRGAGAYICGEESAMIESIEGKRGYPRNRPPYIAELGLFGRPTLNHNVETLY
                     WVPEILKKGAKWFADHGVNGAKGLRSWSVSGRVKKAGVILAPAGITAHELVALAGGME
                     EGESFKAYLPGGASGGILPASMADIPLDFGTLDRYGAFVGSHAIVVFSERDKVADVAI
                     NLLRFFKDESCGQCTPCRVGCDKAVALLERDDWDKELLTELAQTMRDASICGLGQAAP
                     NVFISAMQFFLDEPRPTDVAGRA"
     gene            3815..>6272
                     /gene="fdhF"
                     /locus_tag="VFY74_06630"
     CDS             3815..>6272
                     /gene="fdhF"
                     /locus_tag="VFY74_06630"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX5931408.1"
                     /translation="MTDQLLFTLDGKVVGARPGETIWQVAKREGIDIPHLCWLPQPGY
                     RADGNCRACMVEVEGERVLAASCIRKPTPNMVVKTATERAVKARRMVMELLLADQPSR
                     EDSPDRVSPFWYWAEAQAIESSRFPAHDIIKPDLSHPAMAVNLDACIDCGLCVRACRE
                     VQVNDVIGMAFRGIGARPVFDFDDPMGLSTCVACGECVQACPTGALMPKSIVDPITQH
                     GSREVDREVHSVCPYCGVGCQISYQIKDEKIAFVQGRNGYSNENRLCVKGRFGFDYVT
                     NPQRLMKPLIRKPSLPKGVNIDPANPYTHFREASWEEALDFAASGLARVAETHGNEAI
                     AGFGSAKCTNEEAYLFQKLIRTRFRNNNVDHCTRLCHASSVAALIDGIGSGAVTATFN
                     EVEHADVVMIIGANPTENHPVAATYFKQAAKRGAKLIVIDPRGQAMRDYATYMLQFKP
                     ASDVALLNGIMHVIVEEGLYDVQYLQAYTENFERLKEHLAAYSPESVADITGIKAETI
                     REVARTFGRAERAIIFWGMGISQHVHGTDNARCLIALALMCGQVGRPGTGLHPLRGQN
                     NVQGASDAGLIPMFYPDYADVSAAPVHDKFEALWKTKLSPKPGLTVVEIIHAANKGEI
                     RGMYIQGENPAMSDPDLDHARAGIAKLEHLVVQDIFLTETAMMADVILPSSAWPEKTG
                     TVTNTNRQVQMGRKALALPGDTRDDLWIIIELAKRLGLDWSYTHPSQVYAEMELAMPS
                     LDNITWERLERENSIIYPCPEPGHPGEVVVFGDGFPTESRRAKLVPADILPPAEVPDA
                     EYPLVLMTGRQLEHWHTGAMT"
BASE COUNT         1186 a         2060 c         1980 g         1046 t
ORIGIN      
        1 catcggcgac gaatttctct tcgtcgggct ttgagtgaac gagcaccacg tcgccgtcgc
       61 cgttctcggc attcttgagc gcttgccccg tgccgacggc cacgacccgc acctcgattc
      121 cggttttctt ggtgaagagc ggcaggatgt gatcgaatag gcccgagttc tgcgtcgagg
      181 tggtcgactg cacgacaatg aatttatcgt cggcatgagg tgcgctcgtc gccgctgcaa
      241 gcaggctcgc gccgaggaaa gcccctagca tcattcttcg gctaagcatg aacgacatct
      301 gatcccatcc ttcggttaga gcacaagacc gccggcgagg aagcagcggg cggcctcgct
      361 tcgcggctcg gtgaaaaagc gctttgccaa tccatgttcg acgacctgcc cgtgatgcag
      421 gaacacgaca tcctgcgcaa gcctccgtgc ctggccgaca tcgtgcgtga cgacgatgat
      481 cttgatgccg cgatgctgcg cttcgatcag gagccgctcg atcgcgagcg tcgaggctgg
      541 atcgacgctt gccgtgggtt catccaggaa caggacctca ggttcgaggc tgagcgcgcg
      601 ggcaagacag agccgctgct gctcgccgcc ggagagcacg cgggcgggca tcgcggcgcg
      661 gccttcgaga ccggcaagct tcaaggcccc gtcgacccga attgcgcgct cgcccttgtc
      721 gatgccccgc acgccaagcg catgccggat attggcggcg gcggagcggc gaagcagcac
      781 gggcttctgg aacaccatcg cctgtcggag cgcgatctcg cggtccatgc gacgtcccgc
      841 ccacaggatc tcgccgcgcg tgggcgcgat cagcccatgc atgagcctga gcagcacgct
      901 cttgcccgcg ccgttcggtc ccatgatgac ggtgatgccc ggctgctgaa gcgcgagatc
      961 gacgttgtcg atgacgcgct tgccattcgc ctcataggtg aggcgccgaa gctcgagcgg
     1021 caggagtcca cctctcccgg cgagggcggt cgtgaaagcg cgaggcgatg cggttgggtc
     1081 aagcataggc aaaccgtgcc gcaaaccggc gcacgctcat cagcgccgcg ttgacggcga
     1141 aagcaatgac gaggaggaca atgccgagcg cgagagcaag cgcgatgttt cctttcgacg
     1201 tctcgagcgc gatcgtggtc gtcatcgtcc gcgtgacgtg atcgatattg ccgcccacga
     1261 tcatcacggc gcccacctcg gcgatggcac ggccgaagcc ggcaagcacg gcggtgacaa
     1321 ggcgatatct cccgtcccac agcaacgtcc cgacgatggc ggctgacgag gcgccaagcg
     1381 agcgcagctg ctcgtcatat tcctcgagca ggtcctcgca agcctggcgc gtcagcgcgg
     1441 caacaatagg cgtgacgatg accacttggg ccacaatcat ggcggtcggc gtgtagagca
     1501 gctggagcac gccaaacggg ccggtatttg agagcaggag gtaaacaaag aggccggcca
     1561 caacgggggg gaggcccatc agcgcgctca gaatggccgc aagcgtcccg cgccccggaa
     1621 accgatagac cgcaagtgcg ccgccgagcg caaatccgat caagcttgcg attgcaacgg
     1681 ccgtgaggct cactttgagc gagagcagca cgatcccgag caatttggaa tcgagcgtga
     1741 cgataagctg caaagccgtg tggaatgcct cggtaagttc ggtcatgcga tggcggttct
     1801 tgttttgggc gtggcgacag ggctacaggt tatgtagccc gggactttcg caaaaaaaag
     1861 cgccaagggg gcgtgatccg ccggcggagg cttccgacca ctttggctct tgcggcccgg
     1921 cttgccgatc accattagtc gcggtatcac caagccccat tttgccgccc cctgcctggc
     1981 cctgctggac ggcagatgac ggcgctttcc accgggagaa cgttcggcgt ggacgaaaaa
     2041 acgcgaggca acggcggtcc cgatcggcct gcgcggcatg tgggctcgcg caagaaggcg
     2101 cgcgccttcc cgaagggacg ccagctcgat atcgaagcgc tcgaagagat ccgcgccctt
     2161 ctcggcgacc ggccgcgccg ccacgacctc ctcatcgaat atctgcacct gattcaggac
     2221 cgctatcacg ccatctcggc gccgcacctg aaagcgctct gcgaggagct tcgcttgccg
     2281 caggccgcgg cctacgaggt cgcgaccttc tatgcccatt tcgacgtgct gaaggagggc
     2341 gagacgccgc cgccgcccac gaccatcaga gtgtgcgatt cgatcacctg cgccatcaag
     2401 ggcgccgagg cgctgttcga ggcgctcaaa tcaggtaccg atcgcaaaga cgtgcgcgtt
     2461 ctctacgcac cttgcatggg ccgctgcgac accgcgccgg tcgccgaggt gggacatttc
     2521 catgtcgaca acgccacgcc caaggacgtc atggacgtgg tcacgggcgg caaccgcgag
     2581 ccagcaattc cggcctacga gacgctcgcc gaataccgcg ccaagggcgg ctacgcggtg
     2641 ctcgaaaatt gccgcgccgg aaaactgagc ttcgattcca tcatcaagga aatgaccgac
     2701 gccggcttgc gcggacttgg cggcgcgggc ttccccgcag ggcgcaaatg gcagatcgtg
     2761 cggtcctatc cgggccccag actcatgacc atcaacggcg acgagggcga gcccggcacc
     2821 ttcaaggacc gcttctatct cgagcgagat ccgcaccaga tgttcgaggg cgcgctgatc
     2881 gccgcctggg cggtcgaagc cgaacgcatc tacctttata tgcgcgacga gtatccggcg
     2941 gtgctgcaca ttctcacgca tgagatcgca gcgctcgagg aggcgggcat tatcaagaag
     3001 ggcgacatcg agcttcgccg cggcgccggc gcctatatct gcggcgagga gtcggcgatg
     3061 atcgaatcga tcgagggcaa gcgcggctac ccgcgcaatc gtccgcccta tatcgccgaa
     3121 ctcggcctgt tcggccgccc cacgctcaac cataatgtcg agacacttta ttgggtgccc
     3181 gagatcctga agaagggcgc caaatggttc gccgatcacg gcgtgaacgg cgccaagggc
     3241 ttgcgctcgt ggtcggtgtc ggggcgtgtg aagaaggcgg gcgtgatcct cgcgcccgcc
     3301 gggatcacgg cgcacgagct ggtcgctctg gccggcggca tggaagaggg ggaaagcttc
     3361 aaggcctatt tgccgggcgg cgcctcgggc ggcatcctcc ccgccagcat ggccgacatc
     3421 ccgctcgatt tcggcacgct cgacagatat ggcgccttcg tcggcagcca tgcgatcgtc
     3481 gtcttttccg agcgcgacaa ggtcgccgac gtcgccatca acctgcttcg cttcttcaag
     3541 gatgaatcgt gcggacaatg cacgccgtgc cgggtcggct gcgacaaggc tgtggcgctt
     3601 ctggaacggg acgactggga caaggagctt ctgaccgagc tcgcccagac aatgcgcgac
     3661 gcatcgattt gcgggctggg acaggcggcg cccaacgtct tcatctcggc catgcagttc
     3721 ttcctggatg agccccggcc gaccgatgtg gcgggacgag cctgacggac ttatatgaag
     3781 caaggcggcc tttcggggtc gaggacgagg cagaatgaca gaccagttgc tctttaccct
     3841 ggacgggaaa gtggtgggcg cgaggcccgg cgaaaccatc tggcaggtcg ccaagcgcga
     3901 gggcatcgat atcccccatc tctgctggct gccgcagcca ggctatcggg cagacggcaa
     3961 ttgccgcgcc tgcatggtcg aggtcgaagg cgagcgcgtt ctggccgcct cctgcatccg
     4021 caagcccacg cccaacatgg tggtgaagac ggcgaccgag cgcgcggtca aggcgcggcg
     4081 catggtgatg gagctcctgc tcgccgatca gccctcgcgt gaggattcgc ccgatcgcgt
     4141 ctcgccgttt tggtactggg ccgaggcgca agccatcgag tcatctcgct tccccgccca
     4201 cgacattatc aaacccgacc tgtcgcatcc cgccatggcc gtcaatctag acgcctgcat
     4261 cgattgcggg ctctgcgtcc gcgcctgccg cgaggtgcag gtcaacgacg tgatcggcat
     4321 ggccttccgc ggcatcggcg cgaggcccgt gttcgatttc gacgatccca tggggctctc
     4381 gacctgcgtc gcttgcggcg aatgcgtgca ggcctgcccg accggcgcgc tgatgccgaa
     4441 atcgatcgtc gatccgatca cccagcacgg cagccgcgag gtcgatcgcg aggtccactc
     4501 ggtctgcccg tattgcggcg tcggctgtca gatcagctat cagatcaagg acgagaagat
     4561 cgcgttcgta caagggcgca acggctactc gaacgagaac cggctctgcg tcaaaggccg
     4621 cttcggcttc gactacgtga ccaatccgca gcggctgatg aagccgctca tccgcaagcc
     4681 gagcctgccc aagggcgtga atatcgatcc cgccaacccc tatacccatt tccgcgaggc
     4741 cagctgggaa gaagcgctcg actttgccgc ctcaggcctt gcccgcgtgg cggagaccca
     4801 cggcaacgaa gcgatcgccg gttttggctc ggcgaagtgc accaatgaag aggcctatct
     4861 gttccagaag ctgatccgca cgcgcttccg caacaacaat gtcgatcatt gcacgcggct
     4921 ctgtcacgct tcctcggtgg cggcgctgat cgacggcatc ggctccggcg ccgtcaccgc
     4981 gaccttcaac gaggtcgagc acgccgacgt ggtcatgatc atcggcgcca atcccaccga
     5041 gaatcatccg gtagccgcca cctacttcaa acaggcggcg aaacgcggcg ccaagctcat
     5101 cgtcatcgat ccgcgcggcc aggcaatgcg cgactacgcg acctacatgc tgcaattcaa
     5161 gccggcgagc gacgtggcgc tgctcaacgg gatcatgcac gtcatcgtcg aggagggcct
     5221 ctacgatgtg caatatctcc aggcatacac cgagaatttc gagcgcctga aggagcacct
     5281 cgccgcctac agccccgaat ccgtcgccga catcaccggc atcaaggcag agaccatccg
     5341 cgaggtcgcg cgcactttcg gccgcgccga gcgggccatc atcttctggg gcatgggcat
     5401 ctcacagcac gtgcacggca ccgacaacgc gcgttgcctc atcgcgctcg cgctgatgtg
     5461 cggccaagtc ggccgccccg gcacagggct ccacccctta cgcggtcaga acaacgtgca
     5521 gggcgcttcc gacgccggcc tgatcccgat gttctaccct gactatgccg acgtgagcgc
     5581 cgctcccgtc cacgacaagt tcgaggcgtt gtggaagacg aagctgtcgc cgaagcccgg
     5641 gctcaccgtg gtcgagatca tccacgccgc gaacaagggc gagatcaggg gcatgtatat
     5701 ccagggcgag aacccggcca tgtccgatcc cgacctcgat catgcccgcg ccggcatcgc
     5761 caagctcgag catctcgtgg tgcaggacat cttcctgacc gagacggcga tgatggccga
     5821 cgttatcctg ccgtcctcgg cctggcccga aaagacgggc accgtcacca acaccaaccg
     5881 ccaggtgcag atgggccgca aggcgctcgc gctccccggg gacacccgcg acgatctgtg
     5941 gatcatcatc gagcttgcca agcgcctcgg actggattgg agctacaccc acccgtcgca
     6001 agtgtatgcc gagatggagc tcgccatgcc ctctctcgac aacatcacct gggagcgatt
     6061 ggagcgcgaa aactcgatca tctatccctg tcccgagccc ggccatccgg gcgaggtggt
     6121 ggtgttcggc gacggcttcc ccaccgagtc gcgccgcgcc aagttggtgc ccgccgatat
     6181 tctgcccccg gccgaggttc ccgacgccga atatccgctg gtgctgatga ccgggcggca
     6241 gctcgagcat tggcacacgg gcgccatgac gc
//
LOCUS       DASUQP010000149         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_484397,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000149 DASUQP010000000
VERSION     DASUQP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484397"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..336)
                     /locus_tag="VFY74_06635"
     CDS             complement(55..336)
                     /locus_tag="VFY74_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5931409.1"
                     /translation="MDEVRAEIDRIDIILVDYIGERFTYVDRAWQLKNAPAEAWVPWR
                     IQQVIDRVRARAVENGLPPELAEALWRQMIGWFVQYEEEKLRQQLEGKE"
     gene            complement(382..792)
                     /locus_tag="VFY74_06640"
     CDS             complement(382..792)
                     /locus_tag="VFY74_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5931410.1"
                     /translation="MTETTSKRLLISSGSPYEPIIGFSRAVRVGNIVAIGGTAACSGG
                     KPVAIGDPAAQTRAILAIIAKALEDAGAGLKDVIRTRTYLVDIAHWEAVGRVHGEVFG
                     DIRPVSSMLQVTGFINPDWLVEIEADAVLPANSA"
     gene            complement(789..2276)
                     /gene="gatA"
                     /locus_tag="VFY74_06645"
     CDS             complement(789..2276)
                     /gene="gatA"
                     /locus_tag="VFY74_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5931411.1"
                     /translation="MSKLIELTIAEAREGLRKKSFSSRELTEAHIEAIEKGNDALNAF
                     VLPTPERALEMAKASDARLAKGESGALEGIPLGIKDLFCTRGVRTTACSHILGDFMPT
                     YESTVTANLWAQGAVMLGKLNNDEFAMGSSNETSYYGPVINPWRRKGSNAKLVPGGSS
                     GGSSAAVAARLCMGATGTDTGGSIRQPAAVTGTVGIKPTYGRCSRFGIVAFASSLDQA
                     GPIARTVRDAAILLRAMASHDPKDTTSVDLPVPDYEAAVERGVKGLTIGIPAEYRVPG
                     MAPEIEALWQKGVEWLKAAGAKVKDLSLPHTKYALPSYYIVAPAEASSNLARYDGVRY
                     GLRAKGADVIAMYENTRAAGFGPEVKRRILIGTYVLSAGYYDAYYLKAQKVRTLIKAD
                     FDKAFETVDAILAPTTPGPAFALGEKSGDPVEMYLNDIFTVTVNMAGLPGMSVPAGLS
                     SEGTPLGLQLIGKAFDEETLFRTGRIMEEAAGPLAKPHDWWSIAA"
BASE COUNT          391 a          779 c          701 g          414 t
ORIGIN      
        1 caggggagag ggggagctag cggctaggcc tcacaacgag tgagtagccc gcaactactc
       61 tttcccctcg agctgctggc ggagcttctc ctcctcatac tggacaaacc agccgatcat
      121 ctggcgccac agcgcctcgg caagctcggg cggcagaccg ttctcgaccg cccgcgcccg
      181 cacccggtcg atcacctgct gaatgcgcca ggggacccac gcctcggccg gcgcgttctt
      241 gagctgccag gcccgatcga cataggtaaa gcgctcgccg atatagtcca ccaggataat
      301 atcgatgcgg tcgatctccg ccctcacctc atccattgtc ctgcagtcct ttgccgctcg
      361 cggtgccatg gcgaaactcc cttaggccga gtttgcaggc agaacggcgt ctgcctcgat
      421 ctcgaccagc caatccggat tgatgaatcc cgtcacctgc agcatgctcg aaaccgggcg
      481 gatatcgccg aagacctctc catgcacgcg ccccaccgcc tcccaatgag cgatgtcgac
      541 gaggtaagtc ctggtgcgga tcacgtcctt caggccagcg cccgcatcct caagagcctt
      601 ggcgataatc gccagaattg cgcgggtctg cgccgcggga tcgccgattg caaccggttt
      661 gccgcccgag caggcggcgg tgccgccgat tgcgacgata ttgccgacgc gcacagcgcg
      721 ggaaaagccg atgatcggct cataggggct gcccgaggat atcagaaggc gcttgctcgt
      781 cgtctcggtc atgcggctat gctccaccaa tcgtgcggct tggcgagagg acccgccgcc
      841 tcttccataa tgcggcccgt gcggaacagc gtctcttcgt cgaaagcctt gccgataagc
      901 tggaggccga gcggcgtccc ttcggacgaa agccccgcgg gaacgctcat tcccggcaag
      961 cccgccatat tcaccgtcac cgtaaagatg tcgttgagat acatctcgac cgggtcgccc
     1021 gacttctcgc caagcgcgaa cgcggggccg ggcgtcgtcg gcgcaaggat cgcgtcgacc
     1081 gtctcgaacg ctttgtcgaa gtcggccttg atcaaggttc gtaccttctg cgccttgagg
     1141 tagtaggcgt cgtagtagcc cgccgacagc acataggtcc cgatcagaat gcggcgcttg
     1201 acctccgggc cgaatcccgc ggcacgcgta ttctcgtaca tcgcgatcac gtcggcaccc
     1261 tttgcgcgca acccatagcg tacgccgtcg tagcgggcga ggttcgagga ggcctcggcc
     1321 ggcgccacga tataataaga cggcagcgcg tatttcgtat gcggcaggct taggtccttg
     1381 accttggcgc ccgccgcttt cagccattcc acgcccttct gccacagcgc ctcgatctcc
     1441 ggcgccatgc cggggacgcg atactctgcg gggatgccga tggtgagccc cttcacaccc
     1501 ctctccacgg ccgcctcgta gtctggcaca gggagatcga cgctcgtcgt atccttgggg
     1561 tcgtggctcg ccatcgcccg caacagaatg gcggcgtccc gaacggtacg ggcgatcggc
     1621 ccggcctggt cgagcgagga cgcgaacgcc acgatgccga agcgcgagca gcgcccgtag
     1681 gtcggcttga tgccgacggt gccggtcacg gccgccggct gacggatgga gccgcccgtg
     1741 tcggtccccg tcgctcccat gcagagccgc gcggccaccg ccgccgacga cccgcccgag
     1801 gagccgcccg gcacgagctt cgcgttcgat cctttgcgcc gccaagggtt gatgacggga
     1861 ccgtaatagc tcgtctcgtt cgacgagccc atggcgaact cgtcattgtt gagcttgccg
     1921 agcatcacgg cgccttgcgc ccagagattt gccgtcaccg tcgattcata agtcggcatg
     1981 aagtcgccga ggatgtgcga acaggcggtg gtgcggacgc cgcgcgtgca gaacaggtcc
     2041 ttgatgccga gcgggatgcc ttcgagcgcg cccgactcgc ccttggcgag gcgcgcatcg
     2101 cttgccttcg ccatctcaag cgcgcgctcg ggcgtcggca gcacgaaagc gttgagcgcg
     2161 tcgtttccct tctcgatcgc ctcgatatgg gcttccgtca attcgcgcga agagaacgac
     2221 ttcttgcgca agccttcacg cgcttcggcg atggtcagtt cgatcagctt gctcacgggt
     2281 cccct
//
LOCUS       DASUQP010000150         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_620792,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000150 DASUQP010000000
VERSION     DASUQP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620792"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFY74_06650"
     CDS             <1..727
                     /locus_tag="VFY74_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX5931412.1"
                     /translation="GFDRATGRPIYEQEEMDYDPMPHIVVIVDEMADLMMVAGKDIEA
                     AVQRLAQMARAAGIHLITATQRPSVDVITGTIKANFPTRVSFQVTSKIDSRTILGEQG
                     AEQLLGQGDMLYMAGGGRIMRVHGPFVAEDEVEKVVRHLKKQGTPDYLDAVTDEEESE
                     DEAEEGDELGSSGDDLYDEAVAVVLRDRKASTSYVQRRLGIGYNRAASLVERMENEGL
                     IGPANHAGKREILVGGDEAERPY"
     gene            complement(789..1139)
                     /locus_tag="VFY74_06655"
     CDS             complement(789..1139)
                     /locus_tag="VFY74_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEX5931413.1"
                     /translation="MLYAITKAILSGTIIAAASEVAKRSPALAAMILSLPLVSLLAFV
                     WLWRDTADKEAIAALSQSTFWFMLPTLPMFLVLPALLRNGLNFWPALAIACVLTTGLY
                     LLAVWLLPRLGLVF"
     gene            complement(1224..1562)
                     /locus_tag="VFY74_06660"
     CDS             complement(1224..1562)
                     /locus_tag="VFY74_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX5931414.1"
                     /translation="MLVHLAVLYALLSVGDLTFATAQLVAALCAMTFNFALNNITTYR
                     TARLVGRHFVTGLLIFTVICGAGLLANVGIASFLFRSGEQWWVAGLIGALVGSVWNYA
                     MSRLFVWGRH"
     gene            complement(1511..2368)
                     /locus_tag="VFY74_06665"
     CDS             complement(1511..2368)
                     /locus_tag="VFY74_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX5931415.1"
                     /translation="MKNGEISGESASRRVSAPTGAADISIVVPTLNEADNIKPFIAAI
                     KRVLEPFNWEIVFVDDDSPDGTWKTVQHEAAHDRRIRGIRRVGRHGLASAAIEGFLSS
                     ASPVLVLMDGDLQHEEAILPQMISLIADDHADLVVGSRYLPGGSAAGLSQRHRSWLSF
                     LGLKLAQIGLRTGISDPMSGFFAVKRSVVDVAAPNLSGQGFKILADILASAPGPMRIK
                     EIPYVFRQRAAGESKLEPSSSFNSQSLSWTRPLAATSPRPSFFFRLSAQSECSFTSPY
                     CTRCSLSEI"
     gene            2665..3522
                     /locus_tag="VFY74_06670"
     CDS             2665..3522
                     /locus_tag="VFY74_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX5931416.1"
                     /translation="MISRRTAGLLFALPLTLLLAIGTAPAQEGTSTITKTPPAPSPAA
                     TPPAKPQGLTEPEDAPGPPPGEATGTTGTPAPSEEMAPAAVDEGAGWEAAVEAAQSST
                     PLIGEEQTRAVERINAYFNGITNLQGSFEQVDSTNKTSSGRFYVQRPGKIRFDYAPPS
                     ALRIVADGSHLAIEDSDLKTVEKYPIQSTPFKLLLSEAVDLARDARVVGVESQDGTLA
                     VTLEDKSGDAAGSIRLVFESSSELQLKEWSITDAQGLTTRVTVRDVVPGRKVAADFFT
                     AKEGFQPFR"
BASE COUNT          763 a         1097 c         1152 g          592 t
ORIGIN      
        1 gggcttcgac cgcgccacgg ggcgccccat ctacgagcag gaggagatgg actatgatcc
       61 tatgccccat atcgtggtga tcgtggacga gatggccgac ctgatgatgg tggcgggcaa
      121 ggacatcgaa gccgcggtgc agcgcctcgc tcagatggcg cgcgcggccg gcatccatct
      181 catcaccgcc acgcagcgcc cgagcgtgga cgtcatcacc ggcaccatca aggcgaactt
      241 tccgacgcgg gtgagcttcc aagtcacctc gaagatcgac agccgcacca tcctcggcga
      301 gcagggcgcc gagcagctct tgggtcaagg cgacatgctg tacatggcgg gcggcgggag
      361 gatcatgcgc gtgcacggcc cgttcgtggc cgaggacgag gtggagaaag tggtgcgcca
      421 cctgaagaag cagggcacgc ccgactatct cgatgccgtc accgacgaag aggagagcga
      481 ggacgaagcc gaggagggcg atgagctcgg ctcctcgggc gacgacctct acgatgaggc
      541 cgtggccgtc gtgctccgcg accgcaaggc ctcaaccagc tacgtgcagc gccggctcgg
      601 catcggctat aaccgcgccg cctctctcgt cgagcgcatg gaaaacgagg gcctgatcgg
      661 tcccgccaac catgccggaa agcgcgagat tctcgttggc ggcgacgagg ctgaacggcc
      721 ctattagccg cgagccaggg cgcgcgcgtt ttggccgcag acagctggcc tgcttggccg
      781 ttcgcacctc aaaaaacaag tcccaatcgg ggaagaagcc agacggccag gaggtagagt
      841 cccgtcgtca ggacgcatgc gatcgcgagt gccggccaga aattgaggcc attccgcagc
      901 agtgcgggca gcacgaggaa catcggcagc gtcggtaaca tgaaccagaa cgtcgattgc
      961 gataacgcag cgatggcttc tttgtcggcc gtgtctcgcc acagccacac gaaggcgaga
     1021 agcgacacga gaggtagaga caggatcatt gcagcgagtg ccgggctccg tttcgcaact
     1081 tcggaggcgg ctgcgatgat cgtgccggat aagatcgctt tggtgatggc gtagagcatc
     1141 cggtttcccc ctgtgccaac gatcagagcc aaggattaca cgagcgcatt ggaagcctaa
     1201 tcgacatccg gtgctggctc tactcaatgt ctcccccata cgaacaggcg ggacattgcg
     1261 tagttccaca cggagccgac aagcgcgcca atcagcccgg caacccacca ttgctctcct
     1321 gatcgaaaca gaaaactggc gatgccaaca ttggccaaca agccggcacc gcagatcacg
     1381 gtgaatatca ggaggccggt gacgaagtgg cgtcctacga gccgcgccgt gcgataggtc
     1441 gtgatgttgt tcagcgcaaa gttgaacgtc atggcgcaga gcgccgccac aagctgggcc
     1501 gtcgcgaatg tcaaatctcc gacagagagc agcgcgtaca gtacggcgag gtgaacgagc
     1561 actccgactg cgccgacaat cgaaaaaaga atgaagggcg tggggatgta gcggccaatg
     1621 gtcttgtcca agacaagctc tgcgaattga aggacgacga cggttcgagt ttgctttcgc
     1681 ctgccgcgcg ttggcgaaag acatagggga tctccttgat cctcatcggc cctggggcgg
     1741 aggcaagaat gtcggcgaga atcttgaagc cctgtccgga caggtttggc gccgcgacat
     1801 ccacgaccga gcgcttgacg gcaaagaagc cgctcatggg atcggagata cctgtccgca
     1861 agccaatctg ggcgagcttc aggcccagaa acgacagcca cgagcggtgc cgttgcgaga
     1921 ggccggcggc ggatccgccc ggaagatacc tgctgccaac caccagatcg gcgtgatcgt
     1981 cggcgatgag cgagatcatc tgcggaagga tggcctcctc gtgctggagg tccccgtcca
     2041 tcaggacgag cacgggagag gcagaggaga ggaagccttc aatggcggcc gaggcgagac
     2101 cgtgcctgcc gacgcgccga atacctcgaa tacggcggtc gtgcgcggcc tcatgctgca
     2161 ccgttttcca tgttccgtcg ggcgagtcgt catcgacgaa aacgatctcc cagttgaagg
     2221 gctccaggac tcgcttgatg gcggcgatga acggcttgat attgtcggct tcgttcagcg
     2281 tgggcacgac gattgaaata tcggcggcgc cggtaggtgc agacacgcgc cgtgacgcgc
     2341 tctcgccgct tatctcgccg ttcttcatcg aggcttccgt agccgccttc gcggtgtgga
     2401 cggcaaagcc gcaaaaacag gccgaggcaa tctcacagcg ggttctggaa caattgacac
     2461 cggatttggc accgaacatg cgtaaaccac gagtgaacac gggtcatcaa tctcccgccc
     2521 tgacctcgga aacgcctgga atccggtcat cggggcctcg ctgaaacggc cggtgatgcc
     2581 gtcaagagat tgaccagatt tgcctgaatc atctatcggt tgcctcttag tttcgagcca
     2641 cgccggccgc cggaaggggg gttgatgatt agccgtagaa ccgccgggct gttgtttgcg
     2701 ctgccgctca cccttcttct cgcgatcggg accgcgcctg cccaagaggg cacgagcacg
     2761 atcaccaaga cgcctcccgc gccgtctccc gccgcaacgc ctcccgccaa gccgcaaggc
     2821 ctgaccgaac cggaagacgc gccgggacct ccgcccggtg aagcgacggg tacgaccgga
     2881 actccggcgc catcggaaga gatggctccc gccgctgtcg atgagggggc cggctgggaa
     2941 gcggcggtcg aggcggcgca gtcgtccacc ccgctcatcg gagaggagca gaccagggcg
     3001 gtcgagcgta tcaacgccta tttcaacggc atcaccaatc tccagggcag cttcgagcag
     3061 gtcgattcca ccaacaagac gtcctccgga cgcttctatg tgcaaaggcc gggcaagatc
     3121 agattcgact acgcaccgcc gagcgcgctc cgcattgtgg ccgatgggag tcatctcgcc
     3181 atcgaggact ccgacctgaa gacggtggag aagtatccga tccagtccac gccattcaaa
     3241 ttgctgctgt ccgaggcggt cgatctcgcg cgtgatgccc gggtcgtcgg cgtcgagagc
     3301 caggacggga cgctcgccgt cacgctcgag gacaagtcgg gtgacgcggc aggcagcatc
     3361 aggctggtgt tcgagagcag ttccgagctt cagctcaaag agtggagcat caccgacgcc
     3421 caaggcttga ccacccgggt caccgtcagg gacgtggtac cgggacgcaa agtggcggcc
     3481 gatttcttta ccgcaaagga aggtttccag ccgttccgct aggcgaaacc tttgcgccgc
     3541 aagccatttc acggccattt gacctcgcgg cgcgccgttt gattcatcaa gcgaatcaaa
     3601 tgct
//
LOCUS       DASUQP010000151         3971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_757336,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000151 DASUQP010000000
VERSION     DASUQP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757336"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..2565
                     /locus_tag="VFY74_06675"
     CDS             4..2565
                     /locus_tag="VFY74_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX5931417.1"
                     /translation="MIDFIAVAGAAAFVLWKVSSELPDYEVLAKYEPPVMTRIHANNG
                     ALIAEFSRERRIYVPITAVPSCIIDAFISAEDKNFYQHGGLDVQGIIRAVVTNMSQMQ
                     SGRRSVGASTITQQVAKNFLLSSDQTIERKLKEAILAIRIERAFTKEQILELYLNEIY
                     LGVGAYGVAAAAQSYWDKALNELTLADCAYLATLPKAPSNYDPFKFADRAIARRNWVI
                     DRIVENGFATKEEGEAAKAAPLGVIKRTTGPKIFASEYFAEEVRREILDRFGEDKLYG
                     GGLSVRTTLDPRLQRAARKALIDGFVAFDRRRGGWRGPVKQIELGGDWGTTLAAIQVW
                     PDIAPWRLAVVLEVDKDKAVVGIRPGRSPTGQLLKERETGIVPFEEVKWARPKLERGL
                     GGAPGSVTAVLKPGNVIYVSPREPDLADDGTPKQTPEELKGQWSLQQVPQIGGALVAM
                     DPHSGRVLAIAGGFSFAQSQFDRATQAQRQPGSSFKPLIYTVALDNGYTPSSIIVDGA
                     ICLDQGAGMPQWCPKNYDAGSAAGPSTLRFGIEKSRNLMTVRLARDMGMPIIAEYSRR
                     FGVYDNLMPALSMALGAGETTLLRMVTAYSMLANGGKQVKASFIDRVQDRYGRTIWRH
                     DGRDCSTCAAREWSGQAEPELVDNRTQVIDPMSAFQMTSIMEGVVQSGTAQRLKVLKR
                     PIAGKTGTTNDYKDAWFVGYTPDLAVGVYIGFDQPVSMGHGETGGSLAAPVVRDFLRE
                     ALKDVPPVPFRAPPGVKLVRVNHKSGLPASPGDKSAIVEAFKPGQEPAGSVAEEVMED
                     GQMTQDGYAPPPPDGMAPPPPSGYGPPPPPPPPPPFGRGAAPGDRSLTSGTGGLY"
     gene            2659..3772
                     /gene="prfB"
                     /locus_tag="VFY74_06680"
     CDS             join(2659..2724,2726..3772)
                     /gene="prfB"
                     /locus_tag="VFY74_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_122381344.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5931418.1"
                     /translation="MRAETESVVNDIRESLDLLRRHFDPDQAKARLAILDREAEAPNF
                     WSDSRRAQSLMRERQQLESALNEFDGIVRELDDQVALIELGEEEGDAHVIDEAEQALL
                     RLREEAHKREVEALLSGEADANDAYVEIHAGAGGTESQDWAQMLERMYVRWAEGRGYK
                     VEIIGEHYGEEAGIKSGTVLIKGANAYGWLKTESGVHRLVRISPFDSNARRHTSFASV
                     SVYPAIDDTIEIDIDEKEVRIDTYRSSGAGGQHVNTTDSAVRITHLPTGIVVQCQNER
                     SQHKNRATAWAMLRARLYEAELTRREEEAQAAAAAKSDIGFGHQIRSYVLQPYQLVKD
                     LRTGVESTNPSAVLDGDLDRFIEAALAERIHADQKA"
BASE COUNT          715 a         1310 c         1287 g          659 t
ORIGIN      
        1 gctatgatcg acttcatcgc cgtggccgga gcagccgcct tcgtgttgtg gaaggtgtcg
       61 agcgagcttc ccgactacga agtgctggcg aaatacgagc cgccggtcat gacgcgcatc
      121 cacgccaata acggggcgct gatcgcggag ttctcgcgcg agcggcgcat ctacgtgccg
      181 atcaccgcgg tcccttcctg catcatcgac gccttcatct cggccgagga caagaatttc
      241 taccagcatg gcggcctcga cgtgcagggc atcatccgcg ccgtcgtcac caatatgagc
      301 cagatgcaat cggggcggcg gtcggtcggc gcttcgacca tcacccagca ggtcgccaag
      361 aattttctgt tgagcagcga ccagaccatt gagcgcaagc tgaaggaggc gatccttgcc
      421 atccgcatcg agcgcgcctt caccaaggag cagatcctcg agctttatct caatgagatc
      481 tatctcggcg tcggcgccta tggcgtggcc gccgcggccc agtcctattg ggacaaagcc
      541 ctgaacgagc tgacgctcgc cgattgcgcc tatcttgcga ctttgcccaa ggcgccgagc
      601 aactacgatc cattcaagtt tgccgaccgg gccatcgccc ggcgcaattg ggtcatcgac
      661 cgcatcgtcg agaacggttt tgccacgaag gaagagggcg aggccgcgaa ggccgcgccg
      721 ctcggcgtca tcaagcgcac gaccggcccg aagatcttcg cctcggaata tttcgccgag
      781 gaagtgcgcc gcgagattct cgatcgcttc ggcgaggaca agctttacgg cggcggtctc
      841 tccgtgcgca ccacgctcga tccaaggttg cagcgcgccg cgcgcaaagc gctgatcgac
      901 gggttcgtcg ccttcgaccg caggcgcggc ggttggcgcg gtccggtgaa gcagatcgaa
      961 ctcggtggcg attggggcac cacgctcgca gccatccagg tgtggcccga catcgcgccc
     1021 tggcggcttg ccgtcgtgct cgaagtcgac aaggacaagg ccgtcgtggg gattcgtccc
     1081 gggcgcagcc cgaccggcca gctcctgaag gagcgcgaga ccggcatcgt ccccttcgag
     1141 gaagtgaagt gggcgcggcc gaagctcgag cgtggccttg gcggggctcc ggggagcgtg
     1201 accgcagtgc tcaagccggg caacgtcatc tacgtttcgc cgcgcgagcc cgatctcgcc
     1261 gacgacggga ccccgaagca aacgccggaa gagctcaagg gtcagtggtc gctccagcaa
     1321 gttccccaga tcgggggcgc actggttgcc atggatccgc attcgggccg cgtgcttgcc
     1381 atcgccggcg ggttcagctt cgcgcagagc cagttcgacc gcgcgaccca ggcgcagcgt
     1441 cagcccgggt cgtcgttcaa gccgctgatc tacaccgtcg cgctcgacaa cggctacacg
     1501 ccgtcaagca tcatcgtcga cggcgcgatt tgcctcgatc aaggcgcggg catgccgcaa
     1561 tggtgcccga agaactatga cgccggttct gccgccggtc cctcgacgtt gcgcttcggc
     1621 atcgagaaat cgcgcaacct catgaccgtg cggctcgccc gagacatggg catgccgatc
     1681 atcgccgagt attcgcgccg cttcggggtc tacgacaatc tgatgcccgc gctctcgatg
     1741 gcgctcggcg ccggcgagac gacattgctg cgcatggtca ccgcctactc gatgttggcg
     1801 aatggcggca agcaggtgaa ggcgagcttc atcgatcgcg ttcaggaccg gtacgggcga
     1861 acgatttggc ggcacgatgg gcgcgactgc tcgacttgcg ccgcccggga gtggagcggc
     1921 caagcggagc ccgagctcgt cgacaatcgc actcaggtca tcgatccgat gtcggccttc
     1981 cagatgacct cgatcatgga gggcgtcgtt caaagcggta cggcgcagag gctcaaggtc
     2041 ttgaagcggc cgatcgccgg caagaccggc accaccaacg actacaagga tgcctggttc
     2101 gtcggctaca cgcccgacct cgccgtcggc gtctatatcg gcttcgacca gccggtctcc
     2161 atgggccatg gcgagaccgg cggaagcctt gccgcgcccg tcgtgcgcga cttcttgcgc
     2221 gaagcgctga aagacgtgcc cccggtcccg ttccgcgcgc ctcccggcgt caagctcgtg
     2281 cgcgtgaacc ataagagcgg cctgccggct tcccccggag ataagtccgc gatcgtggag
     2341 gcgttcaagc ccggccagga accggccggc tcggtcgccg aagaggtcat ggaggacggg
     2401 caaatgaccc aagacggtta tgcgccgccg ccgcccgatg gcatggcgcc tccgcctccc
     2461 agtggttacg ggcctccgcc tccacccccg ccgcctccac ccttcggacg gggcgccgcc
     2521 ccgggggacc gttctttgac atcgggtacc ggcggcctct actagtcctt atatagaccc
     2581 gtatcgacct gatccctgcc gcgaggagcc agccggcggc agcgtgtctc atccatccat
     2641 ggctgttttg aggaagcgat gcgcgccgaa accgagtcgg tcgtcaacga catcagagaa
     2701 tccttggacc tgctcaggag gcatctttga ccccgaccag gccaaagcga ggctagcgat
     2761 cctcgatcgt gaggccgaag cgccgaactt ttggagcgac agccgccgcg cgcagagcct
     2821 gatgcgcgaa cgccagcagc tcgagagcgc cctcaacgag ttcgacggca ttgtccgcga
     2881 actcgacgat caagtcgcgc tcatcgagct cggcgaggag gagggagacg cgcacgtcat
     2941 cgacgaagcg gagcaggcgc ttctccgcct gcgcgaggag gcccataagc gggaagtgga
     3001 ggcgcttctc tcgggcgagg ccgatgccaa tgacgcctat gtcgagattc atgccggcgc
     3061 gggcggcacc gagagtcagg attgggccca gatgctggaa cgcatgtatg tgcgctgggc
     3121 ggaagggcgc ggctacaagg tcgagatcat cggcgagcat tacggcgagg aagccggcat
     3181 caagtcgggg accgtgctga tcaaaggtgc caacgcctat ggctggctca agacggagtc
     3241 gggcgtccac cgcctggtgc gcatctcacc cttcgattcc aatgcgcgcc ggcatacgag
     3301 tttcgcctcc gtgtcggtct atcccgcgat cgacgacacg atcgagatcg atatcgatga
     3361 aaaggaagtg cgcatcgaca cctatcgctc ttcgggcgcg ggcggtcagc atgtcaacac
     3421 caccgacagc gcggtccgca tcacgcacct ccccaccggc atcgtggtcc agtgccagaa
     3481 cgagcggtcg cagcacaaga accgggccac ggcctgggcg atgctgcgcg cgcggctcta
     3541 cgaggccgaa ctgacgcgcc gcgaggagga agcgcaagcc gccgccgccg ccaagtccga
     3601 tatcggcttc ggccaccaga tccggtccta tgtgcttcag ccctatcagc tggtgaagga
     3661 cttgaggacc ggcgtggaaa gcaccaatcc ctccgccgtg ctcgacggcg acctcgatcg
     3721 cttcatcgag gcggcgttgg ccgaacgcat ccacgccgat cagaaggcct gagcggctat
     3781 tgtcgggcca tcactggaca gcggcggcga gcggtccgct attccgctgc acgcggcggg
     3841 atagcgcttg cctcgtcttt aggcttgccc tcgggcttgc tgtcgaagct cgccggtttc
     3901 tcgggacggg cctcgacgcg cgggctgcct ccgggtgacg ccttgacctc cgccagggga
     3961 tcgtccgagc g
//
LOCUS       DASUQP010000152         5640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1030354, whole genome shotgun sequence.
ACCESSION   DASUQP010000152 DASUQP010000000
VERSION     DASUQP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030354"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1330
                     /gene="hemW"
                     /locus_tag="VFY74_06685"
     CDS             212..1330
                     /gene="hemW"
                     /locus_tag="VFY74_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5931419.1"
                     /translation="MHWPFCRAKCPYCDFNSHVRHGGIDEARFLTAYLAELSYFASLA
                     PRRRVGSVFFGGGTPSLMQASTVAAILAAIARHWRLETDAEITLEANPTSVEAERFVG
                     YRAAGVNRLSLGVQALDDVSLKALGRQHNVAEALAALGLAKRHFGRVSFDLIYAREGQ
                     TARAWREELGLALGHAADHLSLYQLTIEPGTPFAARHAAGTLRTPNGAEVRAQYLLTQ
                     ELCETAGLPAYEVSNHARPGSESRHNLLYWRGCDYAGIGPGAHSRIGTNGTKRALAAI
                     RSPEDWLAEVERTGRGLASDDPLSAEEAADEYLLMGLRLSEGIDLERFTAIGGRVLDE
                     TRVSALAREGLIVRNGSRLAATPKGRLVLERLIVELAA"
     gene            complement(1336..2529)
                     /locus_tag="VFY74_06690"
     CDS             complement(1336..2529)
                     /locus_tag="VFY74_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX5931420.1"
                     /translation="MRLSVSIVRHVGALRLALAALAVALLVACGGGNGGGFGGPSPGI
                     SGSGGTVKVAVLLPITASGSTPAVAKALKQAAELALFDFDNPNVTLIPKDTKGSPEGA
                     RLAAESALQDGAELIIGPLFAQEVTAAAPVARQGGVPMIAFSTDEKVAGDGVYLLSFL
                     AGRDVPRIVSFALSRGKRKFAELIPQTAYGRIAKTAFATAVSAGGGQAPVRATFPPND
                     SSAMQGPVKQVANAVKSGQAVDALFLPAGREELPELAPHLASSGLTSASVQFIGTGQW
                     DYANIGSDRALVGGWYPAPDPKGWSGFVARYSKTYGSTPPRIASLGYDAVSLAVSLSP
                     NPPGQRYTSAQLTRPSGFSGVDGLFRLLPDGTCERGLAILEVREGGPQVIDPAPRAFA
                     TASSY"
     gene            2750..3676
                     /gene="rsmI"
                     /locus_tag="VFY74_06695"
     CDS             2750..3676
                     /gene="rsmI"
                     /locus_tag="VFY74_06695"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5931421.1"
                     /translation="MQDQRDEEPIRGALGAILARASETLADLIARSLAPGLYLVATPI
                     GNLADISLRALSVLARADLIAAEDTRHSKKLLAHFGLRGALGAYHEHNAERERPLLLA
                     RLRAGQSVALISDAGTPLISDPGYKLVREALDAGLNVVSIPGPSASLGALTSSGLPTD
                     TFLFAGFLPPKSGPRRARLDELRSVPATLIFFETGSRLSKSLADIADVLGPREGAIAK
                     ELTKLHESVTRGTLDRLPGALSGEALRGEFVIVVGPPDAAAAEASDAAIAEQLERALK
                     VESFRDAVRSVAEVLEVSRARVYELGIKLKRE"
     gene            3673..4038
                     /locus_tag="VFY74_06700"
     CDS             3673..4038
                     /locus_tag="VFY74_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX5931422.1"
                     /translation="MTKRQDRVRAYRRGVFAETLVALLFRLKGHRIVARRYETPVGEI
                     DLVALKGRRLAFIEVKRRRTRDDAAWTVTAKLRRRIVRAAQYWLASHPGFAGHDIAFD
                     VVLAAPWSWPRVIANAFPV"
     gene            4277..5245
                     /gene="gshB"
                     /locus_tag="VFY74_06705"
     CDS             4277..5245
                     /gene="gshB"
                     /locus_tag="VFY74_06705"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX5931423.1"
                     /translation="MALKVAFQMDPIERIDIRGDSTFALLLEAQWRGHDVFYYTPANL
                     TLRDGKLVAEGHSLTVEDRPGDHYRLAHPRSVDLADYDVVYLRQDPPFDMAYITTTHL
                     LERIHPRTLVVNDPASVRNAPEKMFVLDFLDLMPPTLVTRSPEQIRAFRDKHKDIVVK
                     PLYGNGGAAIFRIPAGDTNLNPLIELLGITFREPIMVQRYLPEVRKGDKRIILVDGEV
                     AGAINRVPAPDETRSNLHIGGTAEPATLTRRDREICARLGPELKRRGLIFAGIDVIGD
                     YLTEINVTSPTGIRQVSQFGGPDIAAMIWDVVEKKVKAEKRKGRAR"
     gene            5242..>5640
                     /locus_tag="VFY74_06710"
     CDS             5242..>5640
                     /locus_tag="VFY74_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5931424.1"
                     /translation="MSSDPKGLGAVFDAHTDAEFKTRDIDATMATMGSNPHVNHVPVM
                     TGGFGPDGVRSFYETWFIGRWPQDVKITLVSRTVGEAQLVDELILSFTHDCEMPAILP
                     GIRPTGRKVTCPTVVIVGFDQDSKITYEHIY"
BASE COUNT          961 a         1935 c         1844 g          900 t
ORIGIN      
        1 caccgcatgc gcgccttcga gaagctgatg gctgatatct atgttgagag atagcccgcc
       61 ccctcacccc gctcgccgcg atgcggcgag ccacagacaa ccctctccca cgaggggaga
      121 gggtggcgcg ggctcgcgaa gcgaggctgc gccgggtgag ggggaatctc ccgaccccaa
      181 cttcagcgag agcgagccgt tcggcgttta cgtgcactgg cctttctgcc gcgccaaatg
      241 cccctattgc gacttcaatt cccatgtgcg ccatggcggc atcgacgagg cgcggttcct
      301 taccgcctac ctcgccgagc tttcctattt cgcttcgctt gcgccccgac ggcgcgtggg
      361 aagcgtgttc ttcggcgggg gcacgccgtc cctcatgcag gcctcgaccg tcgcggccat
      421 cctcgcggca atcgcccggc attggcggct cgagaccgat gccgagatca cgctcgaagc
      481 caatccaacg agcgtggaag cggagcgctt cgtcggctac cgcgcggcgg gcgtcaaccg
      541 cctgtcgctc ggcgtgcagg cgctcgacga tgtcagcctg aaagccctcg gccgccagca
      601 caatgtggcg gaagccttgg cggcgctagg cctcgccaag cgccatttcg gccgcgtctc
      661 gttcgatctc atctatgcgc gcgagggaca gacggcgcgg gcgtggcggg aggaactcgg
      721 cctcgcgctc ggccacgccg ccgaccatct ctcgctctat cagctgacga tcgagcccgg
      781 tacgccgttc gcggcgcgac atgcggcggg aacgctccgc acgccgaacg gagccgaggt
      841 gcgcgcgcaa taccttctga cgcaggagct gtgcgaaacg gcgggacttc ccgcctacga
      901 ggtctcgaac catgcgcggc cgggatcgga gtcgcggcac aacctgctct attggcgcgg
      961 gtgcgactat gccgggatcg ggccgggcgc tcacagccgc atcggaacga acggcacgaa
     1021 gcgtgccctc gccgcgatca gatctccgga agactggctt gccgaagtgg aacgcacggg
     1081 tcgcggcctc gcgagcgacg atcccttgag cgccgaggag gccgccgacg aatatctcct
     1141 catgggcttg aggctcagcg aaggcatcga tcttgagcgg ttcaccgcga tcggcggccg
     1201 cgtcctcgac gagacgcgcg tgtcggcgct tgcgcgcgaa ggcctcatcg ttcgcaatgg
     1261 cagccggctc gccgccacgc caaaagggcg gctcgtgctc gagcggctga tcgtcgagct
     1321 cgcggcctga ccccttcaat aggacgaggc ggtggcgaag gcgcgcggcg ccgggtcgat
     1381 cacctgcggg ccgccctcgc gcacttcgag gatggcgagg ccgcgctcgc acgttccgtc
     1441 ggggagcagc cggaacagtc catcgacccc ggaaaagccg ctcggccgcg tgagctgcgc
     1501 gctcgtgtaa cgctggccgg ggggattggg cgacagcgag accgcaaggc tcaccgcatc
     1561 atagccgagg ctcgcgatgc ggggcggggt ggagccgtag gtcttggaat agcgcgcgac
     1621 gaaaccgctc catcccttgg gatcgggcgc cgggtaccag ccgccgacga gcgcccggtc
     1681 gctgccgata ttcgcatagt cccactggcc cgtgccgatg aactgcacgc ttgcgctcgt
     1741 gagccctgac gaggcgagat gcggcgcaag ctccggaagc tcctcgcgcc cggccggaag
     1801 gaagagcgcg tcgaccgcct gccccgactt gacggcgttt gcgacttgct tcaccgggcc
     1861 ctgcatcgcg ctcgagtcgt taggcgggaa cgtcgcgcgc acaggggcct ggccgccgcc
     1921 ggcggagacg gctgttgcga acgcggtctt ggcgatgcgg ccataggccg tttgcgggat
     1981 gagctcggcg aacttccgct ttccccgcga cagcgcgaag gagacgatac gcggcacatc
     2041 gcgcccggcg agaaagctca agagatagac gccatcgccc gccaccttct cgtcggtgga
     2101 aaaggcgatc atcggcacgc cgccttgccg cgccacaggc gcggcggccg tgacctcctg
     2161 ggcgaagagc gggccgatga tgagctcggc gccgtcttgc agcgcgcttt ccgctgcaag
     2221 ccgcgcgccc tccggcgagc ccttcgtgtc cttggggatc agcgtcacat tcggattgtc
     2281 gaagtcgaac agcgcaagct ccgccgcctg cttcagcgcc ttggccacgg cgggggtcga
     2341 gcccgaggcg gtgatgggaa ggaggaccgc caccttcacc gtgccgccag agcccgagat
     2401 gccggggctt ggcccgccga acccgcctcc gtttccgccg ccgcaggcca ccagcaacgc
     2461 taccgccaag gcggcgagag cgagcctcaa ggctccaacg tgccgaacga tgctgaccga
     2521 aagtcgcatg gccccttgct tcaacgcggg cgggcttttc gcgcctgcgc cttcatggtg
     2581 gcgccagtgg cgatcgcggg ctcgccctct tgacgtcccc gcccatcctc tcccgtcgcc
     2641 cgacccaagg cttgcccgca cccaaggctc aaacgtgatg gacgcgaggc gattatatca
     2701 cgtaacgttt ttgcgcctag ccagccttca gttttcgggc cccgcgcgcg tgcaagacca
     2761 gcgagacgag gaaccaatca gaggcgctct cggcgcgatc ctcgcgcgcg cgagcgaaac
     2821 gctcgcggac ctcatcgcaa ggtcgctcgc acccgggctc tatcttgtgg caacgccgat
     2881 cggcaatctc gccgacatct cgctccgcgc gctttcggtg ctggcccgcg cagacctcat
     2941 cgccgcggag gacacgcgcc actcgaagaa gctgctggcg catttcggct tgcgcggcgc
     3001 gctcggtgcc tatcacgagc acaacgccga acgcgagcgc ccgctccttc ttgcccgact
     3061 ccgcgccgga cagtcggtgg cgctcatctc cgatgccggc acgccgctca tctctgaccc
     3121 cggatacaag ctcgtgcgcg aagcgctcga tgcgggactt aacgtcgtca gcatccccgg
     3181 gccgtcggct tcgctcggcg ctctcacaag ctcgggcctt ccgaccgaca cgttcctctt
     3241 tgccggcttc ctgccgccga aatcgggtcc gcggcgggcg cggctcgatg agctcagaag
     3301 cgtccccgcg acgctcatct ttttcgagac gggctcgcga ctctccaaaa gccttgccga
     3361 catcgcggat gtgttggggc cgcgcgaggg ggcaattgcc aaggaactga ccaagctgca
     3421 cgagagcgtg acgcgcggga cgctcgaccg gctgcccggc gcgctttcgg gtgaggcatt
     3481 gaggggtgag ttcgtgattg tcgtcggacc gcccgacgcc gccgcggccg aggcgagcga
     3541 cgcggcgatt gccgagcagc tggaacgggc gctgaaagtc gagagcttcc gcgacgcggt
     3601 gcgctcggtt gcggaagtgc tggaggtgag ccgcgcccgg gtctacgagc tcgggatcaa
     3661 gctcaagcgc gaatgacaaa acggcaagac cgggtcagag cctatcgccg gggcgtcttc
     3721 gccgaaacgc tcgttgcgct tctgtttcgg ctgaaaggcc accgcatcgt tgcgcggcgt
     3781 tacgagacgc cggtcggcga gatcgacctc gtggcgctca agggcaggcg gctcgccttc
     3841 atcgaggtga agcggcggag gacgcgcgat gacgccgcct ggacggtgac ggcgaaactg
     3901 cggcggcgga tcgtgcgggc cgcgcaatat tggctcgcaa gtcaccccgg cttcgccggt
     3961 catgacatcg ccttcgacgt cgtcctggcg gcgccctgga gctggccccg cgtcatcgcc
     4021 aacgcatttc cggtttagcg accaccacgc agcccctaag cagcggtctc gtcgaagcgt
     4081 agcaaccaac gcccgctctc cccttggggg agagcacacg cgcctcttgg cgcgtggtga
     4141 ggggttaggc gcgaagcgcc gtgatcggaa accccctcac ccggctcgcc gcacggcgga
     4201 aagggtgagg ggtcagcata cgatggagac tttggctaca ctccgactat aagagagcgc
     4261 cgtgtagggg agagatatgg ccctcaaagt cgccttccag atggacccga tcgagcgcat
     4321 cgatatcagg ggcgattcga ccttcgcgct gctgctcgaa gcgcaatggc gcgggcacga
     4381 cgtgttctac tacacgcccg ccaatcttac gctccgcgac ggcaagctcg ttgcggaggg
     4441 ccacagcctc accgtcgagg accggcccgg cgaccattac cgcctcgcgc atccgaggag
     4501 cgtcgatctc gccgattacg acgtggtcta tctccgccag gacccgccct tcgacatggc
     4561 ctatatcacc acgacgcacc tgcttgagcg catccatccg aggacgctcg tggtcaacga
     4621 tcccgcctcc gtgcgcaatg ccccggaaaa gatgttcgtg ctcgatttcc tcgacctcat
     4681 gccgccgacc ctcgtcacgc gctcgcccga acagatccgc gccttccgtg acaagcacaa
     4741 ggacatcgtg gtgaagccgc tctacggcaa tggcggcgcc gccatcttcc gcatccccgc
     4801 gggcgacacc aatctcaatc cgctgatcga gcttctcggg atcaccttcc gcgagccgat
     4861 catggtgcag cgctatctgc ccgaggtgcg caaaggcgac aagcgcatca tcctcgtcga
     4921 tggcgaggtc gccggcgcga tcaatcgcgt gcccgcgccc gacgagacac gctccaatct
     4981 gcatatcggc ggcacggcgg agcctgcgac gctgacaagg cgcgacaggg agatctgcgc
     5041 gcggctcggc cccgagctca agcggcgcgg actgatcttc gccggaatcg acgtgatcgg
     5101 cgactacctc accgagatca acgtcacctc gcccacaggc atcaggcaag tgtcccagtt
     5161 cggcggtccc gacatcgccg ccatgatctg ggacgtggtc gagaaaaagg tgaaggccga
     5221 gaagcgcaag ggtcgcgcgc gatgagctcc gatcccaaag gcctcggcgc ggtgttcgac
     5281 gctcataccg atgccgagtt caagacgcgc gacatcgacg ccaccatggc cacgatgggc
     5341 tcaaatccgc acgtcaacca tgtcccggtg atgaccggcg ggttcgggcc ggacggggtg
     5401 cgcagcttct atgagacctg gttcatcggc cgctggccgc aagacgtgaa gatcacgctc
     5461 gtctcgcgta cggtcggcga ggcccagctg gtggacgagc tgatcctatc cttcacccac
     5521 gattgcgaga tgccggcgat tctgcccggc atcaggccga ctggacgcaa agtgacatgc
     5581 cccaccgtgg tgatcgtggg attcgaccaa gacagcaaga tcacgtacga gcatatctat
//
LOCUS       DASUQP010000153        13786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1302475, whole genome shotgun sequence.
ACCESSION   DASUQP010000153 DASUQP010000000
VERSION     DASUQP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13786
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302475"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..692)
                     /locus_tag="VFY74_06715"
     CDS             complement(291..692)
                     /locus_tag="VFY74_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX5931425.1"
                     /translation="MSAVSRRLVAEGVGTALLLAAIVGSVIMAERLAGGNVAIALLCN
                     SLASGAMLYVLITILAPISGAHFNPAVTLAFLITGSIGGIAALAYMAFQVIGAVLGSR
                     TSCLRCRYLSFPRPDEPRLIFGWRKPWRPLV"
     gene            complement(696..1223)
                     /locus_tag="VFY74_06720"
     CDS             complement(696..1223)
                     /locus_tag="VFY74_06720"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX5931426.1"
                     /translation="MKIVSSREPLNVLFLCTGNSARSIMAEAILNRLGRGKFRAFSAG
                     SQPAGYVHPLALQMLRNAHYDVTGLRSKPWDELATADAPKLDFVFTVCDNAANEVCPI
                     WPGQPMTAHWGLPDPAKAKGTEAEKATIFADTMRMLNQRIGIFVSLPLDKLSRLSLQE
                     QLDEIGKTKAETETT"
     gene            complement(1234..1569)
                     /locus_tag="VFY74_06725"
     CDS             complement(1234..1569)
                     /locus_tag="VFY74_06725"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5931427.1"
                     /translation="MEINSAVQALSALGQESRLRVFRLLIRRGPKGTPAGDIAEQLKI
                     PANTMSAHLAVLSRAGLVASRKQGRSVIYSVDVAGTRKLLAFLVEDCCRGKPKVCQPL
                     IASALADCC"
     gene            1622..2488
                     /locus_tag="VFY74_06730"
     CDS             1622..2488
                     /locus_tag="VFY74_06730"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX5931428.1"
                     /translation="MKATNDQAIRAAVRDHYTSVANRASKEPRPLDPKVEDDCCAPTC
                     CGETPPSPNDAKCCSPSCCDENSPSLQIGYSAEELSNVPEGADLGLGCGNPQAIAALK
                     PGEIVLDLGAGAGFDAFLAARQVGPSGRVIGVDMTPAMLEKARWNADKAGCGNVEFRL
                     GEIEHLPIANASVDVIISNCVINLSPDKPAVFAEAFRVLKPGGRLAISDVVATKPLPD
                     TLAKSVAAHARCISGALELGNLKAMLTASGFSDIRITIDEQSREFIRDWLRNSGAEDY
                     VASATIQATKNE"
     gene            complement(2532..3254)
                     /locus_tag="VFY74_06735"
     CDS             complement(2532..3254)
                     /locus_tag="VFY74_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931429.1"
                     /translation="MTINLEMDAEPRNETARSRTAHGGAPPGSRNGRYVDGNFTKEAI
                     AERRLLRRLITGSGPGKMTSESVVNHEHPNRVSIEVEKGEGRYAVKAPANQDKRDWTR
                     RLQKALGSRSKAFIVASLDRLLAACHLPRQLIPTSTSVSSALALIESLEPKNEIEAAL
                     AVEIACLHAACGTLLGRLADACLDRPTIAAANAVAKLERALHSAINTFYRVKHGNTQV
                     IRIERIEIQSGAQAVLGPVMRP"
     gene            3887..4537
                     /locus_tag="VFY74_06740"
     CDS             3887..4537
                     /locus_tag="VFY74_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX5931430.1"
                     /translation="MSAPPGSASRLSQPPAVEFYKGDLPRGRFRGRAIAIDTETLGLN
                     PHRDRLCLVQLSEGDGRAALVQFAGTYEAPELKRLLADESVLKIFHFGRFDMAVLKHY
                     FGVMPRPVYCTKVASRLARTFTDRHGLKDLCKELLGIELSKQQQSSDWGAAELTQEQL
                     NYAASDVLHLHALREKLDAMLRREGREGLAHACFEFLPIRAELDLLGWSDIDPFAH"
     gene            4649..5476
                     /gene="lptC"
                     /locus_tag="VFY74_06745"
     CDS             4649..5476
                     /gene="lptC"
                     /locus_tag="VFY74_06745"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX5931431.1"
                     /translation="MAADANVVAGGRRYRIRTTEERMRAFERAQRHTKLVNVLRKALP
                     LFAVLVLATYFISSRLAVGVSIGDMTASVEGIEVADGNLRMVNPKLEGADKRNGKYVI
                     GADYADQDIKNPNLIKLHAIKADLAALDGGWSRMTATRGRFDNKTGRLVMQDKIDIAT
                     SSGVSGALTHATLDTKNQIIRSHRPVSFVLPNGTVRANALTFYSAKHTLTFRGKVAVH
                     IVRPPKQAKPADTAPAAAPQNVAPPPETGVSEEPPLPPMPPESGARTDATGAAASVP"
     gene            5473..6303
                     /locus_tag="VFY74_06750"
     CDS             5473..6303
                     /locus_tag="VFY74_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX5931432.1"
                     /translation="MTGARHVLLMSMLRIFAGVAPLSAALLIAPPTMAQTLTNSFGGL
                     SESSEDPIDIESDVLVVHDKEKRATFRGNVKAVQGSTTLRAKELNVHYVGGDKLAAVG
                     KKEGGGEAPEAKVADAKGDAKSAKSDQAQITKIEAKGEVVITSDKDQTTTSDWALYDV
                     PSQLVTVGGNVVLTQGQNVLKGDRLVIDLKTGESRFDNTGNEAAGGRIRALFMPKDAA
                     KGGAPKDAKPGEANPAAQEKAASSRNKPDKDPSDETDGDPFNEAPWPLTPDTRPDGFR
                     "
     gene            6359..7180
                     /gene="lptB"
                     /locus_tag="VFY74_06755"
     CDS             6359..7180
                     /gene="lptB"
                     /locus_tag="VFY74_06755"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX5931433.1"
                     /translation="MLSFPGRQATTPQKGTTQATGPVKDPKAEANPEFGRLIVRSVVK
                     SFKKRTVVRGVSLDLCRGEAVGLLGPNGAGKTTVFYMITGLIPADEGLIKIDGTDVTK
                     LPMYRRARLGIGYLPQEASIFRGLTVEQNILAVLETVEPDQRERQRQLDALLEEFRIT
                     RLRTSPAMRLSGGERRRVEIARSLASRPSFMLLDEPFAGIDPIAVNDIRALVRHLTQR
                     GIGVLITDHNVRETLDLVSRAYIIHDGMVLTHGTAQEIIANEDVRRVYLGDQFKI"
     gene            7207..8766
                     /gene="rpoN"
                     /locus_tag="VFY74_06760"
     CDS             7207..8766
                     /gene="rpoN"
                     /locus_tag="VFY74_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5931434.1"
                     /translation="MAITTKLELRQAHSLVMTPQLQQAIKLLQLSSVELASFVEAELE
                     RNPLLERADPDDTSGVAEPASEPGPPAKDEAEAQGAASENEAGEGDDGDWIDLETGAS
                     GTEDLDVEPQDVFPDGDGAPLAALNSAGTTQFGPSGVATGGEVPNLESYVAGERTLRD
                     HLTEQLMLAFADPAHRLIGYHLIDMTDEAGYLCGDLAGLAELLGAPLELVEETLRVMQ
                     GFDPSGVFARDLRECLMLQLQERDRCDPAMAAMVANLNLLAVHDLSALKRAAKVSDED
                     LLDMIAEVKRLNPKPGLSYGSAPAQVIVPDVLVRALPDGSFHVELNSETLPRVLVNRS
                     YYATVSKSATRKEDRTYLADCFQTANWLVKSLDQRARTILKVAREIVRQQDAFLTYGV
                     RHLRPLNLKTVADAIAMHESTVSRVTANKYMATNRGLFELKYFFTSAIAATSEGDAHS
                     SESVRDRIRAMIEAEPAGGVLSDDKIVERLKDDGIDIARRTVAKYREALRIPSSVQRR
                     RQKRLAPCARV"
     gene            8999..9622
                     /gene="raiA"
                     /locus_tag="VFY74_06765"
     CDS             8999..9622
                     /gene="raiA"
                     /locus_tag="VFY74_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5931435.1"
                     /translation="MTIQVTGKNLDVGEALRSYVQERVTHTVEKFIGRETMGHVRIEK
                     ERGEFRTDCTIHLWQGMSLEAHGTAPDAYQSADRACERLEKRVRRYKRRLKRHGGESS
                     RKHTPAADYVIQAAQDGREEHDEHNPVIIAEAETPVHEMAVSDAVMQMDLSDRSIVVF
                     RNASHGEINVVYRRDDGNIGWIDPAGPLSSVGAAVAQQRTKKARKRS"
     gene            9671..10138
                     /gene="ptsN"
                     /locus_tag="VFY74_06770"
     CDS             9671..10138
                     /gene="ptsN"
                     /locus_tag="VFY74_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX5931436.1"
                     /translation="MLLTNVIAPNSVVPLLRAKNKKQLLQDLSARAARVTGVPERDIF
                     DILLQRERLGSTGLGHGVATPHGKLQGLKRIVGIFARLAEPIDFDALDGQKVDIVFLL
                     LAPEGAGADHLKALARISRLLRDATAAEKLRASKDAAALYAVLTEGEPSSHAA"
     gene            complement(10176..10448)
                     /locus_tag="VFY74_06775"
     CDS             complement(10176..10448)
                     /locus_tag="VFY74_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 domain-containing protein"
                     /protein_id="HEX5931437.1"
                     /translation="MNKFEEEEESRQMQISRRELAQLGDGEVAYIKQLDSDAAERLFP
                     ALSDAPKGIDLFAVLGADGTPLALTDSRSAAIANAIENNLVPVSVH"
     gene            complement(10511..10924)
                     /locus_tag="VFY74_06780"
     CDS             complement(10511..10924)
                     /locus_tag="VFY74_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX5931438.1"
                     /translation="MPRMTLLDDSLWLGLSAPRGPSGAGKPDAGYPPYNVELLPEEPE
                     ALRITLAVAGFGANQLEVTTENGELSIRGKQHEEQNRDFLHRGIAARQFKRNFRLASG
                     VEVRKAELDNGLLVIDLVRPRKERRVMKIGIGAAD"
     gene            11175..11384
                     /locus_tag="VFY74_06785"
     CDS             11175..11384
                     /locus_tag="VFY74_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEX5931439.1"
                     /translation="MIWYVNRRSHDAPDGRTAGTAFKTLAQAIEAAKPGDTILIAPGT
                     YDQDLPAEVSAARAAGIVVAVMGSE"
     gene            11587..11811
                     /locus_tag="VFY74_06790"
     CDS             11587..11811
                     /locus_tag="VFY74_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931440.1"
                     /translation="MIRVIFASLLLALALIFAHQPAKAEWLCGVERCVWVTYDVDEPA
                     YALAWGPPIYPSCYWRRGIFGRWKMICPGR"
     gene            complement(11939..12310)
                     /locus_tag="VFY74_06795"
     CDS             complement(11939..12310)
                     /locus_tag="VFY74_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931441.1"
                     /translation="MSVSAATELTESSRAHEAHALAFRGLAATAAYLLVTQLPSPAFL
                     LLVVLLVGLLFGDRISRGGDMAPIYTVGLTTFLILLGLGLSPLPQDSGSLFVVRVFDV
                     MVAVVYAIGMANLLRSAFGAR"
     gene            complement(12300..13391)
                     /locus_tag="VFY74_06800"
     CDS             complement(12300..13391)
                     /locus_tag="VFY74_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007637103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX5931442.1"
                     /translation="MAAKSDAKKNDDPVRRWTLIVLGLIVVLFLYSLIADRLTPYTSQ
                     ATVQAFVVRMAPEVAGRVIEVNVVDNQRVAAGDVLFSIDPQPFTIALQEADARLAGVG
                     QSIGASTAAVSSAQERLVTAQAKRDNTREQVSRIFQLVEKGVYAKARADQATAQIQSA
                     EAGVREAEAELERAKQELGPQGADNPQFREALAAVEKAKLDLVRTTVAAPSDGVITNL
                     QLTDGEFVAVGQAALTFIDTSDVWINANFRENSLENIKPDAPAELVLDTLPGRIFTAK
                     VESVGWGVSQGSIDPETGLPKISESMGLVRNPQRFAVKIEPNRKDYEPGSVRYGSQAN
                     VIVYATDNPIVNAIGALWIRLVAILTYVS"
     gene            complement(13415..>13786)
                     /locus_tag="VFY74_06805"
     CDS             complement(13415..>13786)
                     /locus_tag="VFY74_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX5931443.1"
                     /translation="VAGPGKKVVEDIAVPEDPHTIAVYPLAVPYLLNPVGITILIIAS
                     SEVVSVASAALVLALVLLVGAFDYLVFANIDKLAKRLNPTSLVISEVVFGILLTAVAV
                     QLAVSGLGSLGIIDATHGSTH"
BASE COUNT         2720 a         4248 c         4243 g         2575 t
ORIGIN      
        1 ctctgcaccc tggacgctaa agccttcggt caccgacaag tgggattacc tcaattctcg
       61 ttagcctgcg ttaaagctac accgaatagc cattggctgt gccagaatga atgctggggt
      121 cccggcggga tcgataccag aaaacgtatc cgtaagtgac ctcgctatcg taacggcggg
      181 attggcaaaa ctggttgatg ccgtgaacca atacgcagaa acgatataga ggcccacggc
      241 ggtggggata gcatccggct tccaccgtag tgcgccgaga atcgtcaaga tcaaaccaaa
      301 ggtcgccacg gcttccgcca accaaagatt agtctcggct cgtccggtcg tggaaagctc
      361 aagtatcggc agcgcaaaca tgacgtgcgc gagccaagga cggcgccaat aacctgaaaa
      421 gccatatacg cgagggcggc gatgccgccg atggaacccg taatgaggaa agccagcgtg
      481 acagccgggt taaaatgggc gccagatatt ggtgccagaa tcgtaattag cacgtagagc
      541 attgcgcctg aggcaagcga attacaaagc agcgcaatag ctacgtttcc tcctgcaagt
      601 cgctcggcca ttatgaccga gccaacgatt gcggcgagga gcaacgccgt gccaacgcct
      661 tccgccacca aacgccggga tacagccgac acgacctaag ttgtctcagt ttcggctttt
      721 gttttgccga tctcgtcaag ctgttcttgc aaagagagcc tactgagctt gtctaacggc
      781 aaactgacaa aaattccgat gcgttgattt aacatccgca tggtgtcggc aaagatggtc
      841 gccttttctg cctctgtgcc tttcgctttg gccggatcgg ggagtcccca atgagccgtc
      901 atgggctggc ccggccagat aggacaaacc tcgttcgctg cgttgtcgca aaccgtaaag
      961 acgaaatcga gcttgggagc atccgcagtg gctaactcat cccaaggttt tgatcgcagg
     1021 cccgtcacgt cgtagtgcgc atttctcagc atttgtaaag cgaggggatg tacataaccc
     1081 gcaggctgac tgcccgcgct aaaggctctg aacttgccac gtccgagtcg gtttagaatg
     1141 gcttcggcca tgatcgagcg cgccgagttg cctgtgcaaa ggaaaagcac gttcagcggc
     1201 tctctgctgc tgacgatctt cataaacacc tccttagcaa caatccgcca acgccgaagc
     1261 gatgagtggc tggcagacct tcggttttcc tcggcaacaa tcctcgacca aaaatgcgag
     1321 cagcttgcgc gttcctgcga catcgaccga gtaaatcacg gaccggcctt gcttgcgtga
     1381 ggcaaccagc cccgcccgcg acagcacggc tagatgcgct gacatcgtgt tcgcggggat
     1441 tttaagctgc tctgcgatgt cgcccgcagg tgttccttta ggacctcgcc tgattaaaag
     1501 acggaaaacg cggaggcggc tttcctgacc tagcgcactt agtgcttgaa cggcggagtt
     1561 tatttccata gtataagaaa tatcgaatta tgctatttgt caagaaggat catccaaagc
     1621 aatgaaggcg acgaacgatc aagctatccg cgctgcggtc cgcgaccatt atacctctgt
     1681 ggcgaacaga gcctcaaagg aaccgcgccc actcgatccc aaggttgaag atgattgctg
     1741 tgctccgacc tgctgtggcg aaacccctcc atctccaaac gatgcgaaat gttgttcacc
     1801 gtcgtgctgc gacgaaaact ccccttcatt gcaaatcggg tactcagccg aggaactctc
     1861 aaacgtcccc gaaggcgccg atttggggct tggctgcggc aacccccaag ctatcgccgc
     1921 gctgaaacca ggcgagattg tcctcgatct cggagcgggt gcaggctttg acgcttttct
     1981 tgccgcgcgc caagtcgggc cgagtggccg agtaatcggc gtcgatatga cgcccgcaat
     2041 gcttgagaag gcccgttgga acgccgataa ggcaggatgc ggaaatgtcg agtttcggct
     2101 cggtgagatc gagcatctcc caattgccaa tgcgagcgta gacgtgatca tctcaaattg
     2161 cgtcatcaat ctttctcctg acaagcctgc cgttttcgcc gaggcgttca gggtgctgaa
     2221 accaggcggt cgtctagcta tctccgatgt cgttgcaacc aagcctctcc cagacacgct
     2281 cgcaaagtcc gtggcagcgc atgcccgctg catttcaggt gcactagaat taggcaattt
     2341 gaaagcaatg ctgacggcgt ctggcttttc ggacatccgc atcaccattg acgagcagag
     2401 ccgtgagttc atccgcgatt ggctacgcaa ctctggtgct gaggattacg tagcatcggc
     2461 cacaattcag gccaccaaga atgagtgaaa aacgcaatcg gcctgaaagg gatgttgagt
     2521 gagagcatgt cctaagggcg catgaccggc ccgaggacag cttgcgcacc gctttggatt
     2581 tcaattctct ctattcggat cacctgcgtg ttgccgtgct tgacgcggta gaaggtgttg
     2641 atcgccgagt gcaacgcgcg ttcgagtttg gcgacagcat ttgccgcagc aatcgttggc
     2701 ctgtcgaggc aggcatcagc taggcggcca agcaaagtgc cacaggccgc gtgcagacat
     2761 gcaatttcca ctgccaaggc tgcctcaatc tcgttcttgg gctccaacga ctcgattagc
     2821 gccaacgccg acgagacgct tgtcgatgtg gggataagct ggcgaggcaa atggcaggcc
     2881 gccaggagtc gatcaagaga ggcgacaatg aaagccttag accgggagcc aagtgccttc
     2941 tgcaagcggc gtgtccaatc ccttttgtcc tgatttgccg gtgcctttac cgcgtatcgg
     3001 ccttcacctt tctccacctc gatcgagacg cggttcggat gctcgtgatt gactacggat
     3061 tcgctagtca tctttcctgg ccccgatccg gtgataagcc tgcgcagcaa ccggcgctcc
     3121 gcgatagctt ctttggtgaa atttccatcg acataacggc cattcctgga accaggtggc
     3181 gcgccgccgt gcgccgtgcg ggagcgagcc gtctcatttc tcggttcagc atccatttcc
     3241 aggttgattg tcatttttca tgtcccccct accagaacag agctacagac cccttacgac
     3301 gattctgtac ggctgatcga actatgctgt tcagcatctg accctgagtt cagatcgtga
     3361 cgggcatagc ttcgccttct caggcgaagc cgtgtcctga cccttgagac gagtcgctta
     3421 gagacatagg cggatcaggg cagcctcaat tgaggtgagc cggggtcgtg ctgtcggctc
     3481 ttacgccccg cttcatacgg cgcgggctca gccgccacag ccgatccacc gaccgtgaca
     3541 gcgcctgagg gacggtgcca accggagccc tctcctgacc atcagcgcgg gtgggcgcta
     3601 gtggtcttga tgccgatgcc cttcggcctt tgtgacacgg gtcctcgcaa tggtctggct
     3661 aacctcacgg cccgtggcgc gtagtgcgcc ggctatgaaa ccagtgcacc taggcaatgg
     3721 gcggtgggtc ggctcggttt tgtgttgtgg tgcgggtgtg gttgaaaatt cctcttgtgc
     3781 ctcaaaggca cttttgcgcc cgcaccctgg cgcaaactct tgatttgtcc cgttagtggg
     3841 ccaattctcc tgggcaccat tcccgctttg ttaagcgcca cgcggtatga gtgcgccgcc
     3901 agggagcgct tcacgcttga gccagccacc ggccgtagaa ttctataagg gcgatctgcc
     3961 gcgaggccgc ttccgcggcc gcgccatcgc catcgacacc gagacgctgg ggctcaatcc
     4021 ccatcgcgat cggctgtgcc tcgtgcagct ttcggaagga gacggccgcg ccgccctggt
     4081 gcagtttgcc gggacctatg aggcgcctga gctcaaacgc ctgctcgcgg atgagagcgt
     4141 gctcaagatc ttccatttcg gccgcttcga tatggcggtg ctcaagcact atttcggggt
     4201 gatgccgcgg ccggtctatt gcaccaaggt ggcctcgcgg ctcgcccgga ccttcaccga
     4261 ccggcacggc cttaaggatc tctgcaagga actgctcggg atcgagctgt ccaaacagca
     4321 gcaatcttcg gactgggggg cagccgagct cacccaggag cagctcaact atgccgcgag
     4381 cgacgtgttg catctgcacg cgcttcggga gaaactggat gccatgcttc ggcgcgaagg
     4441 gcgcgaaggc ctcgcgcacg cgtgttttga gttcttgccg atccgggccg agctcgatct
     4501 gctgggttgg tccgatattg atcccttcgc ccattgagct gccgaatacg gcgcgccggg
     4561 cgaggcagac ccgctgtcgc aatgtgatca aagtcctgcg gctagctggt ccggacagtt
     4621 aacgtgcctt gaggttgggc gggaaaaaat ggcggctgat gcgaacgttg ttgccggcgg
     4681 ccggcggtat cggatccgca cgaccgagga gcgcatgcgc gccttcgagc gcgcccagcg
     4741 ccacaccaag ctcgtcaacg ttttgcgcaa ggcgctcccg ctcttcgcgg tgctggtgct
     4801 ggccacctat ttcatttctt cgcgtcttgc cgtgggcgtc agcattggcg acatgaccgc
     4861 gagcgtcgaa ggcatcgaag tcgccgacgg caatctgcgc atggtcaacc cgaagctcga
     4921 gggagccgac aagaggaacg gaaaatatgt catcggcgcc gactacgccg accaggacat
     4981 caagaacccg aacctgatca agctgcacgc cataaaggcc gatctcgccg cgctcgatgg
     5041 cggctggtcg cgcatgacgg ccacgcgggg cagattcgac aacaagaccg gccgcctcgt
     5101 gatgcaggac aagatcgaca tcgccacgag ctcgggcgtc tccggcgccc tgacacatgc
     5161 gacgctcgac accaagaacc agatcatccg ctcgcatcgg ccggtctctt tcgtcctgcc
     5221 caatggaaca gtcagggcca acgcgctcac cttctactcg gccaagcata cgctcacctt
     5281 tcgcggcaag gtcgccgtcc acatcgtgag gccgccgaag caggcgaagc cggccgacac
     5341 cgcccccgcc gccgccccgc agaacgtggc tccgccgccg gagacggggg tctccgagga
     5401 gccgccgctg cctccgatgc cacccgaaag cggcgcccgc accgatgcga ccggcgctgc
     5461 tgcgagcgtc ccatgaccgg cgcccggcac gtcttgctca tgtccatgct ccgcatattt
     5521 gccggtgtgg cgccgctctc cgccgccttg ctgatcgctc cgccaacgat ggcgcagacg
     5581 ctcacgaatt cgttcggcgg cctttcggag agctcggagg atccgatcga tatcgagtcg
     5641 gatgtgctcg ttgtgcacga caaggagaaa cgcgcgacct tcaggggcaa tgtcaaagcg
     5701 gtccagggca gcaccacgct ccgcgcaaaa gagctcaatg tgcactatgt cggtggcgac
     5761 aagcttgccg ccgtcggtaa gaaggagggt ggcggggaag ctcccgaggc caaggttgcc
     5821 gatgccaaag gcgatgccaa atctgcaaaa agcgatcagg cccaaatcac caagatcgag
     5881 gcgaaaggcg aagtcgtcat caccagcgac aaggatcaga ccacgaccag cgattgggcc
     5941 ctctacgacg tgccgtcgca gctcgtcacc gtcggtggca atgtggtgtt aacgcaaggc
     6001 cagaatgtgt taaaggggga ccgactcgtc atcgatctga aaacgggtga aagccggttc
     6061 gacaatacgg gaaatgaagc cgccggaggt cgtatccgtg cgctgttcat gccgaaggat
     6121 gctgccaagg gcggtgcgcc gaaagacgcc aagccgggtg aggccaaccc cgccgctcag
     6181 gaaaaggcgg cttcgagccg caacaagccc gataaggatc cgagcgacga gaccgacggt
     6241 gaccctttca acgaggcgcc atggccgctc actccggaca cgcggcctga cggcttccgt
     6301 tgaggagagt ttaacccgcc ggcgcttcat ctagagggcg aaaggggtct tgggcaaatt
     6361 gctgtcattt cccggccgcc aggcaaccac gccacagaag ggcacgactc aagcgaccgg
     6421 gccggtcaaa gaccctaagg ctgaggccaa tccggaattt ggccgcctca tcgttcgttc
     6481 cgtcgtcaag agcttcaaga agcgcaccgt tgtccgcggc gtcagcctcg atctctgccg
     6541 cggcgaggct gtcgggctgc ttgggccgaa cggggcaggc aagaccaccg tgttctacat
     6601 gatcacgggc ctcattcccg ccgacgaggg cttgatcaag atcgacggca ccgacgtcac
     6661 gaaactgccg atgtatcggc gggcgcggct cggcatcggc tatctgccgc aagaagcatc
     6721 gatcttcaga gggctcacgg tggagcagaa cattctcgcc gtgctggaaa ccgtagagcc
     6781 ggaccagcgc gaacgccagc gccagcttga cgcgttgctc gaggaattcc gcattacccg
     6841 gctcaggacc tcgcccgcca tgcggctctc gggcggcgag cggcgccggg tcgagatcgc
     6901 ccgttcgctt gcgtcgcgcc cgtccttcat gttgctcgac gagcccttcg ccggcatcga
     6961 ccccatcgcc gttaacgaca ttcgcgcgct cgtgcgtcac ctgacccagc gcggtatcgg
     7021 cgtcttgatc accgatcaca acgtgcgcga aacactcgat ctcgtcagcc gtgcctatat
     7081 aattcacgat ggcatggtgc tgacccacgg caccgcgcag gagatcattg ctaacgagga
     7141 cgtacggcgc gtctatctcg gcgatcagtt caagatctga gtcgccccgc ggatttcagt
     7201 tcgacaatgg cgatcaccac aaagctcgaa ctccgtcagg cccacagcct ggtcatgacg
     7261 ccgcagttgc agcaggcgat caagctgctc cagctttcca gtgtcgagct tgcgagcttc
     7321 gtcgaggccg agctcgagcg caatccgctg ctcgagcgcg ccgatcccga cgacacgagc
     7381 ggggttgccg aacccgcgag cgagcccggt ccgcccgcca aggacgaggc ggaggctcaa
     7441 ggtgccgcga gcgaaaacga agcgggcgaa ggcgacgacg gcgattggat cgatcttgag
     7501 acgggcgcga gcgggaccga ggacctcgat gtcgagccgc aggacgtctt ccccgacggc
     7561 gatggcgccc cccttgccgc gcttaatagc gcgggcacga cccaattcgg gccgagtggg
     7621 gtcgcgaccg gcggggaagt tccgaacctc gaatcttatg tcgccggaga gagaacgctt
     7681 cgggatcacc ttaccgagca gctcatgctc gccttcgccg atcctgcgca ccgcctgatc
     7741 ggctatcacc tcatcgatat gaccgacgag gcgggctatc tctgcggcga cctcgccggt
     7801 cttgccgaat tgctcggcgc gccgcttgag ctcgtcgagg agacgcttcg cgtgatgcag
     7861 ggcttcgacc cgagcggcgt cttcgcccgc gatttgcgcg agtgcctcat gctccagctg
     7921 caagaaaggg atcgctgcga tccggctatg gcggccatgg tcgcgaacct caatcttctc
     7981 gccgtccacg atctctcagc gctgaagcgc gccgccaaag tctccgacga ggatttgttg
     8041 gacatgatcg cggaggtgaa gcggctgaac ccgaagccag gtctcagcta cggctcggcg
     8101 cccgctcaag tcatcgttcc cgacgtgctc gtgcgtgcgc ttcccgacgg cagctttcat
     8161 gtcgagttga acagcgagac cctgccgcgg gtgctcgtca accggtccta ttatgcgacc
     8221 gtctccaaat cggcgacacg caaggaggac cgaacctatc ttgccgattg cttccagacg
     8281 gcgaattggc tcgtcaagag cctcgaccag cgcgcccgca ccatcctcaa agtggcgcgg
     8341 gagatcgtcc gtcagcagga cgccttcctc acctatggcg tccggcatct cagacccttg
     8401 aacctcaaga ccgtggccga cgcgatcgca atgcacgagt cgaccgtaag ccgggtcacc
     8461 gccaacaaat acatggcgac caatcgcgga ttattcgagc tgaaatattt cttcacctcg
     8521 gcgatcgcgg cgacgagcga aggcgatgcc cactcctccg aatcggtgcg cgaccgcatc
     8581 cgcgccatga tcgaggccga gcccgccggc ggcgtcctgt ccgacgacaa gatcgtcgag
     8641 cggctgaagg acgacggcat cgacatcgcg cgccgcaccg tggcgaagta ccgcgaggcg
     8701 ctgcgcatcc cgtcttcggt gcagcgccgg cggcagaagc gcctcgcccc ttgcgcgcgc
     8761 gtctagatcg ccctaaatct gtggataaga acgagaatcc tgtaaagagg gacggcgcaa
     8821 atagttggtt tgacaccccg ggacgtgcgt tctagcttcc cgccccgcgg gtggagaccg
     8881 gaaggcaatc ggttccaaag cgcgccggtt tcgctttgac aaagttcggt tccgcggagg
     8941 tcgcggcaag cgccccggta acgcgtggga aaggccagtc gacagtcttc cgccgacaat
     9001 gacgatacaa gtaaccggaa agaacctgga cgtcggtgag gcgttacgca gttacgtcca
     9061 ggagcgcgta acccataccg tcgagaagtt catcgggcgc gagaccatgg ggcatgtgcg
     9121 catcgaaaag gagcgtggcg agtttcgaac cgactgcacg attcatttgt ggcaaggcat
     9181 gagtctcgag gcgcatggca ccgcgcccga cgcctatcag agcgccgatc gcgcctgtga
     9241 gcggctcgag aagcgggtcc ggcgctacaa acggaggctg aaacggcatg gaggagagag
     9301 ttcccgcaaa cacacgccgg cggcggacta cgtcattcaa gccgctcaag acggtcgtga
     9361 ggagcacgac gagcacaatc ccgtcatcat tgccgaagcc gaaacgccgg tgcatgaaat
     9421 ggcggtaagc gacgccgtga tgcagatgga tttatccgac cggtcgattg tcgtgtttcg
     9481 caacgcaagt cacggggaaa tcaacgtggt ctaccgccgc gacgacggca atatcgggtg
     9541 gatcgatcca gccgggccgc tgagctcagt gggcgcagcg gtcgcgcagc aacgaaccaa
     9601 gaaggcgcgc aagcggagct aggcggacga tccgctttgg cccgcgaccg cccatcggag
     9661 gggaatcaag atgctgctaa cgaatgtcat cgctcctaac tctgtcgtgc cgctgctcag
     9721 ggcaaagaac aagaagcagc tgcttcagga tctgtccgcg cgtgccgccc gcgtcaccgg
     9781 cgttcccgaa cgcgatatct tcgacatcct gttgcagcgc gagcggctcg gctcgaccgg
     9841 gctcggccat ggcgtcgcca cgccgcacgg caagctccaa ggactgaagc ggatcgtcgg
     9901 catcttcgcg cgccttgccg agccgatcga cttcgacgcg ctcgacggcc agaaggtcga
     9961 tatcgtcttc cttctgctcg cgcccgaggg tgccggcgcc gatcatctca aggcgctcgc
    10021 ccgcatctcg cgcctgttgc gcgacgcgac ggcggcggag aagctccgtg cctcgaagga
    10081 tgccgccgcg ctttacgcgg tgttgaccga gggcgagcct tcgtctcacg ccgcctgagc
    10141 cgcttcactc atcgcaacaa cgaaacgtgg aaatgctagt gcacgctcac cggcacgaga
    10201 ttgttctcga tggcgttggc gatcgcggcg ctccggctgt cggtgagcgc gagcggggtt
    10261 ccgtcggcgc cgagcacggc gaagaggtcg atgcccttcg gcgcgtcgga cagcgcggga
    10321 aataggcgct cggcggcgtc cgaatcgagc tgcttgatat aggcgacctc gccgtcgccg
    10381 agctgagcga gttctctgcg gctgatctgc atctgacgac tttcttcttc ttcttcgaac
    10441 ttgttcatcg gacctatgtt cctcgtaacg agggggatgg cgaagctacg gagcgcgtat
    10501 ggcgaaggtt tcaatcggcg gcaccgattc cgatcttcat cacgcgcctt tccttgcgcg
    10561 gacggacgag atcgatgacg agcaggccgt tgtcgagctc tgccttgcgg acctcgaccc
    10621 cgcttgcgag ccgaaaattt cgcttgaatt gccgcgcggc gatgccgcga tgcagaaagt
    10681 cgcggttctg ctcttcgtgc tgtttgccgc gaatggaaag ttcgccgttc tcggtggtca
    10741 cctcgagttg attggcgccg aagcctgcga cggcgagcgt gatgcgcaac gcctcgggct
    10801 cttccggcag gagttccaca ttgtaaggcg gatagccggc gtcgggcttg ccggcgccgc
    10861 tcggcccgcg gggtgcggac aggccgagcc aaagactgtc gtcgaggagc gtcatgcgcg
    10921 gcatcgagcg aaccctttcc cgtaacgggg cgcgccatgc cggggcttag gcgaggccct
    10981 gacggcgcct cgtagacaag acccggcccc gccgcgggcg ccgagccttt ggttttccgc
    11041 cttcggatat ggggaacggc ggcgccaatt caagcgcggc cgagcctccg atcacccgct
    11101 cgtgcttggc tcctaccgct tgcgcgcggc ttgacttaaa tttgcaaggc cgagcctttg
    11161 ctgggccgcg aacgatgatc tggtacgtca atcggagaag ccacgacgcg ccggacggcc
    11221 ggaccgccgg cacggccttc aagactctcg cgcaggcgat cgaagccgcc aagcccgggg
    11281 acaccatctt gattgcgccg ggcacctacg atcaggatct gccggcagag gtcagcgccg
    11341 cgcgcgcggc cggcatcgtc gtcgcggtga tggggagcga gtagggccgt gatgcgttct
    11401 tgggcgctgt cgccgccatc gcgccgatac gcggccgatg caagtttgtc ccaaagttta
    11461 tcgttaacgc tcgatcgtgt tttttgccgt cgtgcggacg agaatcgggg tcggcgggat
    11521 ggactcaagc ccgcgcccgg gttagagcga ttgcgcggca atgaccatca ttgttagggg
    11581 gttcaagtga tcagagtcat ttttgcaagt ttgctcctcg cgctcgcgct catctttgcg
    11641 catcagccgg cgaaggccga atggctgtgc ggtgtcgaac gatgcgtctg ggtcacctat
    11701 gacgtcgacg agccggccta tgcgctggcc tggggaccgc cgatctaccc aagctgctac
    11761 tggaggcggg gcatcttcgg acgctggaag atgatctgcc ccggaaggtg atctgcacgc
    11821 gctagggcag ccgcttccgc tccgttaagt tcactgccga tctgatggcc ggtgccttgt
    11881 cgcgccgcgc tttggcgcgc cgtgcgcttg cgccgaggat ggctgaaaga tggtggcgtc
    11941 agcgggcgcc gaacgccgag cgcaacaggt tggccatgcc gatggcatag acgaccgcca
    12001 ccatgacgtc gaacacgcgg acaacgaaga gggaaccgct gtcctgcggc aagggcgaga
    12061 gcccgaggcc gagcaagatc aggaaggtcg tgaggccgac cgtgtagatc ggcgccatgt
    12121 cgccgccgcg cgaaattcga tcgccgaaga gaagcccgac aagcaagacg accagcagaa
    12181 gaaacgcggg ggaaggaagt tgcgtgacga gaagataagc ggcggtcgca gcaaggcctc
    12241 tgaaggccag cgcatgggcc tcgtgcgcac gcgaagactc tgtcagctct gtcgcggcac
    12301 taactgacat aggtcagaat cgctacgaga cgtatccaca atgccccaat ggcgttcacg
    12361 atcggattgt cggtcgcata gacgatcacg tttgcctgcg agccgtagcg cacgctgccg
    12421 ggttcataat ccttgcgatt gggctcgatc ttgacggcga aacgctgcgg gttgcgaacg
    12481 agccccatcg attcactgat cttcggcagc cccgtctcag gatcgatcga accttgcgac
    12541 acgccccagc cgacgctttc gactttggcg gtaaagatac gcccggggag cgtgtcgagc
    12601 acgagctccg ccggcgcgtc gggtttgatg ttctccaaac tgttttcgcg gaaattggcg
    12661 ttgatccaga cgtcgctggt atcgatgaac gtcagggccg cttggcccac ggcgacgaat
    12721 tcgccgtccg tcagctgcaa gttggtgatg acgccgtcgg aaggcgcagc gaccgtggtg
    12781 cggacgaggt cgagcttggc cttctcgacg gctgcgagcg cctcgcggaa ttggggattg
    12841 tcggcgcctt gagggccgag ctcttgcttg gcgcgctcga gctcggcctc ggcctcccgg
    12901 acgccggcct cggccgattg gatctgcgcc gtcgcctggt ccgcgcgagc tttggcgtag
    12961 acgcccttct cgacgagctg gaagatgcgc gaaacttgct cgcgcgtatt gtcgcgcttg
    13021 gcttgagccg tgaccaaacg ttcctgtgcg gagctgaccg ccgccgtgct ggccccgatc
    13081 gattggccga cgccggccag cctggcatcc gcctcctgaa gggcgatggt gaagggctgc
    13141 ggatcgatgc tgaagagcac gtccccagcc gccactctct gattgtcgac gacattgacc
    13201 tcgatcaccc gcccggcgac ttccggcgcc atccgcacca cgaaggcctg aacggtcgct
    13261 tgagaggtat agggcgtgag gcgatccgcg atcagcgaat agaggaacag gaccacgatc
    13321 aggccgagaa cgatcagcgt ccatcgcctg acggggtcat cgttcttttt ggcgtcactc
    13381 ttcgcggcca tcgcctgcac ccccgtcgtc gtgcctagtg ggtggacccg tgcgttgcgt
    13441 cgatgatccc gagcgacccc agtccgctaa ccgcgagttg gaccgcgacc gcggtcaaca
    13501 ggatcccgaa cacgacttca gaaatgacca ggctggtggg attgaggcgt ttcgccaatt
    13561 tgtcgatgtt ggcgaagacc agatagtcga acgctccaac caacaacacg agggccaata
    13621 ccagtgccgc gctcgccaca gagacgacct cgcttgaggc gatgatcaat atcgtgatgc
    13681 cgaccggatt cagcagatac ggcaccgcga gcgggtagac cgcgattgtg tgtggatcct
    13741 ctgggaccgc aatatcctcc acgaccttct tgcccggccc agccac
//
LOCUS       DASUQP010000154         3604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1167611, whole genome shotgun sequence.
ACCESSION   DASUQP010000154 DASUQP010000000
VERSION     DASUQP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167611"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..1326
                     /locus_tag="VFY74_06810"
     CDS             451..1326
                     /locus_tag="VFY74_06810"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012557920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5931444.1"
                     /translation="MTKVAFIGLGVMGFPMAGHLKTRGGHDVVVFNRTRAKADAWVSR
                     FGGRSAATPAAAADGADFVFTCVGNDDDLRQVTLGPDGTFAALAPGAIAIDHTTASAR
                     IARELYAAAKTRGGNAIDAPVSGGQSGAENGTLTVMAGGDDEAFRNAAPVIAAYARAC
                     NLIGPPGAGQLTKMVNQICIGGLIEGLAEALHFARHADLDIEKVIDTISKGAAQSWQM
                     ENRWRTMIDGKFDFGFAVDWVRKDFALIFEEARSNGARLPVTALVDQFYAEIQAMGGA
                     RWDTSSLIARLERER"
     gene            1375..2268
                     /gene="folD"
                     /locus_tag="VFY74_06815"
     CDS             1375..2268
                     /gene="folD"
                     /locus_tag="VFY74_06815"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX5931445.1"
                     /translation="MTAKMIDGKAIAAKLSQSIADAVKGVTAEHGIEPGLAVVLVGEN
                     PASEIYVRSKTAKTAATNMRSLLYKLPATARPDELFALIEQLNDDPAVHGILVQLPLP
                     PQIDSHKVIEAIAPAKDVDGFHPLTAGLLSTGLPGIVPCTPLACIILAKQVHGSLAGK
                     EAVVIGRSNIVGKPLAQLLLMENATVTIAHSKTRDLPAVCRRGDIVFAAVGQPEMVRG
                     DWIKPGATVIDVGINRVAGADGTSKLVGDVAFDEVSQVAGAITPVPGGVGPMTIACLM
                     LNTLKAACAQAGLEPPPVSGT"
     gene            complement(2273..2746)
                     /locus_tag="VFY74_06820"
     CDS             complement(2273..2746)
                     /locus_tag="VFY74_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5931446.1"
                     /translation="MLDRMDRKILDILQKDCTLPVAEVGKRVGLSTTPCWRRIQKLEE
                     LGVIQARVALLDAKKVNVGVTVFVSITTSHHTQDWLDRFHAALSEFPEVVEFYRMSGQ
                     VDYLLRVVVPDIERYDTFYKKLIGKIELSDVSSAFAMEQIKFSTALPLDYASEGG"
     gene            complement(2861..3484)
                     /locus_tag="VFY74_06825"
     CDS             complement(2861..3484)
                     /locus_tag="VFY74_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX5931447.1"
                     /translation="MTATAAELQRLLAEIRACRHCVEAPEGKPLPHAPRPVLRASSTA
                     RLAVCSQAPGTKVHQSGTPFSDRSGERLRAWMGVENEEFYDESRVAIVPMGFCFPGQD
                     ANGADLPPRRECAPLWHRELFAALPQLELVLAIGSYAHRWHLGDAAGLTLEETMRDWR
                     KHLRRRMRPRVLPLPHPSWRNNAWLKQNAWFETELLPVLRREVRRLL"
BASE COUNT          664 a         1168 c         1126 g          646 t
ORIGIN      
        1 cctgcgtgat cggccacata gtgacaggca agtagtccaa gggaaataag gggcgcggaa
       61 ttgtctttat ttcaaaagac aattccatcg cttctgctaa tccgatgcat tgagtctctg
      121 cacctgctac gccctcggtc aaggaccagc aagttaaagt ctctatcatt gcaaatttaa
      181 cggctgtgta cgaagcgcgc cattgcattt cgcctccgtg agcgatttaa gctccctata
      241 tggctttttt gcgcgcttgc ctgggaggtg tgctggccaa gccagcggac gcccagtccc
      301 ctttgaggca acaaatagtt aaccaaaccg ctaacgagcc tgatgacatg tcatcaaccg
      361 aattccagtt ttaactctta gggaaacttt cataaaacaa atcactttgg cagagccctt
      421 cccgtcgact tcgaagtcct tggacaagcg atgaccaagg tcgcttttat cgggctcggc
      481 gtgatgggtt ttcccatggc cgggcatctc aagacgcgcg gggggcacga cgttgtcgtg
      541 ttcaaccgca cccgcgccaa ggccgacgcc tgggtttcgc ggtttggcgg acggtccgcg
      601 gcgacacccg ccgccgcggc ggacggcgcg gacttcgtct tcacctgcgt cggcaatgac
      661 gacgacctcc gccaggtgac gcttggaccc gacggaacct tcgccgcgct cgcgcccggt
      721 gcgatcgcca ttgaccacac cacggcctcg gccaggatcg cgcgcgagct ctacgccgcc
      781 gccaagacgc gcggcggaaa cgcgatcgat gcgcccgtgt cgggtggcca gtcgggcgcc
      841 gagaacggga cgctcacggt gatggcggga ggcgacgatg aggcgtttcg caacgccgcg
      901 cccgtgatcg ccgcctacgc gcgggcgtgc aatctgatcg gacctccggg cgccggccag
      961 ctcaccaaga tggtcaacca gatctgcatc ggcggcctga tcgaggggct cgccgaggcg
     1021 ctccactttg cgcgtcacgc cgatctcgac atcgagaagg tcatcgacac gatctcaaag
     1081 ggcgccgcgc agtcttggca gatggagaac cgctggcgca cgatgatcga cggcaagttc
     1141 gatttcggct tcgccgtgga ttgggtgcgc aaggacttcg cgctcatctt cgaggaggcg
     1201 cggtcgaatg gcgcgcgcct gccggtcacc gcgctggtcg accagttcta cgccgagatc
     1261 caggccatgg gaggcgcgcg atgggacacg tcgagcctga tcgcgcggct cgaacgcgag
     1321 cgctgaaagc cgctcgcccc ttttttgaca gtcctccaac cgacaggtag cagcatgacc
     1381 gccaaaatga tcgacggcaa agccatcgcg gcaaaactca gccaatccat cgccgacgcg
     1441 gtcaaggggg tcacggccga acacggcatc gagcccggac ttgccgtggt gctcgtcggc
     1501 gagaatcccg ccagcgaaat ctacgtgcgc agcaagaccg ccaagacggc ggcgaccaat
     1561 atgcgctcgc tgctgtacaa attgccggca acggcccgcc ccgacgagct cttcgcgctg
     1621 atcgagcagc tgaacgacga tccggccgtg cacgggatcc tcgttcagtt gccgctgccg
     1681 ccgcagatcg actcgcacaa ggtgatcgag gcgatcgctc cggccaagga cgtcgacggg
     1741 ttccatccgc tgaccgcggg tctcctgtcc accggtctcc ccggcatcgt gccctgcacg
     1801 ccgcttgcct gcatcatcct cgccaagcag gttcatgggt cgctcgcggg aaaagaagcg
     1861 gtcgtgatcg gccgctcgaa catcgtcggc aagccgctcg cgcagctgtt gctgatggag
     1921 aacgcgacgg tgacgattgc gcactccaag acgcgcgact tgccggccgt atgccggcgc
     1981 ggcgatatcg tcttcgccgc cgtcggtcag ccggagatgg tgcgcggcga ctggatcaag
     2041 cccggcgcca ccgtcatcga cgtcggcatc aaccgcgttg cgggcgcgga cgggacatcc
     2101 aagcttgtcg gcgatgtggc cttcgacgaa gtctctcagg tcgccggcgc catcacgccc
     2161 gtgccgggcg gcgtcggtcc gatgaccatc gcctgtctca tgctgaacac gttgaaggcc
     2221 gcctgcgcgc aagccgggct ggaaccgccg cccgtttccg ggacgtgagg tctcaaccgc
     2281 cctccgaggc atagtcgagg ggcagcgcgg tcgagaactt gatctgctcc atggcgaagg
     2341 ccgagctcac atcggacagc tcgatcttgc cgatgagttt cttgtagaag gtgtcgtagc
     2401 gctcgatgtc gggcacgacc acgcgcaaga gatagtcgac ctgcccgctc atgcggtaga
     2461 actcgaccac ttcggggaac tcgctgagcg ccgcatggaa gcggtcgagc caatcctggg
     2521 tgtggtggct cgtggtgatg gacacgaaca cggtcacccc gacattgact ttcttggcgt
     2581 cgagcagggc gacgcgcgct tggatcactc ccaactcttc gagcttctgg atgcgccgcc
     2641 agcacggcgt ggtcgaaagc ccgacccgct tgccgacttc ggccaccggc aaggtgcaat
     2701 ccttctgcaa aatgtcgagg atcttgcggt ccattcggtc gagcatggcg ttgtcggcaa
     2761 tcctcttgcg ccgttactgc atattacgga acgacgttct aatatcgacg aaaatcgatc
     2821 agtctggtag attatttttc tacctccggc aagaagcatt ttaaagcagg cgcctcacct
     2881 cgcggcgcag cacggggagc agctccgtct cgaaccaggc attctgcttg agccaggcat
     2941 tgttgcgcca ggagggatgc ggcagcggga ggacgcgcgg gcgcatcctt cgccgaagat
     3001 gcttccgcca atcgcgcatc gtctcctcga gcgtgagccc tgccgcgtcg ccgagatgcc
     3061 atcgatgcgc gtaagacccg atcgcgagca cgagttcgag ctgcggcagc gcggcgaaca
     3121 gctcacgatg ccacagcggc gcacattcgc gccgcggcgg gaggtccgcg ccgttcgcat
     3181 cctggccggg aaagcagaac cccatgggca cgatggcgac ccgagactcg tcgtagaact
     3241 cttcgttctc gactcccatc cacgcccgca agcgttcgcc cgaccggtcg ctgaagggcg
     3301 tgcccgattg gtgcaccttg gttcccggcg cctggctgca cacggcgagc cgcgccgtgg
     3361 agcttgccct cagcaccggg cgcggcgcat gcggcagagg tttcccttcc ggcgcctcga
     3421 cgcagtggcg gcaagcccgg atttctgcca gcaggcgctg aagctcggcc gcagtcgccg
     3481 tcatagatcg aggacaagcc cgtcgcgggc gattgtgtaa cgcgaggcgt cgaccttgtc
     3541 cgtcgccgca agcatggcct cccccatatg ggtgagaagc atccgtttcg cgccgagccg
     3601 cgcg
//
LOCUS       DASUQP010000155        12877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_77620,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000155 DASUQP010000000
VERSION     DASUQP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77620"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1133)
                     /gene="thiL"
                     /locus_tag="VFY74_06830"
     CDS             complement(114..1133)
                     /gene="thiL"
                     /locus_tag="VFY74_06830"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX5931448.1"
                     /translation="MVDRGERAEGQRLGEFDIIARYFAPLATDASALGLRDDAAVLRP
                     PESQEIVISCDTIVEGVHFLKGDPPDSIAHKALAVNLSDLAAKGARPYAYLLALALPE
                     EPSPAWLEAFASGLRALQTRTGIALVGGDTTRIPGPLSITITALGLVPQGHAVLRLGA
                     KRGDRLYVTGTIGDAYLGLRALREPALANDWGFTEEDLAFVIERYRRPLPHSELAIVM
                     RNFAQGAIDVSDGLVGDIGKLASVSHVGAVIEAGLVPFSSAGEKALKREPQLLAALLA
                     AGDDYEIVAAVPQGSAAAFEAEATARGTPVTPIGRLESPEAGLLVLGADGKSLALDVR
                     GYAHF"
     gene            complement(1126..1623)
                     /gene="nusB"
                     /locus_tag="VFY74_06835"
     CDS             complement(1126..1623)
                     /gene="nusB"
                     /locus_tag="VFY74_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5931449.1"
                     /translation="MAKESRSTPRASSRSAARLAAVQALYQMDMTGIDLNQVIAEFES
                     HRLGQEIEGWQYAEAESGFFRDLVAGVVREQLRIDPLIDKQLAEGWRLTRVDSILRAI
                     LRAGAYEILVRGDVPVRVVISEYVDIAHAFFAEEEPKAVNGILDRLGHRARPQEFAAG
                     GSRDG"
     gene            complement(1629..2099)
                     /gene="ribH"
                     /locus_tag="VFY74_06840"
     CDS             complement(1629..2099)
                     /gene="ribH"
                     /locus_tag="VFY74_06840"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5931450.1"
                     /translation="MTRKGPHSVLIIEARFYEDIADELARGAIEELEAHRMAYERISV
                     PGSLEIALALSLALANGFVGRAGRHRGCVALGCVIRGETTHYDIVARESAAGLHQLAV
                     QHAVPIGFGILTCDSREQAWERASVDGRNKGRDAARACLTLMQLEQHFSSAKRR"
     gene            complement(2104..3222)
                     /gene="ribB"
                     /locus_tag="VFY74_06845"
     CDS             complement(2104..3222)
                     /gene="ribB"
                     /locus_tag="VFY74_06845"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX5931451.1"
                     /translation="MTIAPKTRHHQFLSPIEEVVEDARNGRMFILVDAEERENEGDLV
                     IPAQMATPDAINFMAKHGRGLICLALPPERAKQLHLELMSRSNRARHSTAFTVSIEAR
                     EGVSTGISAHDRSHTVSVAIDPTKDYRDLMTPGHVFPLVAKDGGVLVRAGHTEAAVDL
                     ARLAGLYPAGVICEIMNDDGTMARLPDLIAFADRHGLKIAAIADLIAYRLRYDHLVKV
                     VAETTVESAFGGQFDLKVYATTVDPAEHVALIKGDVASGGPVLVRVHALNTMDDVISI
                     GTHRETLVHKAMDIIGREGRGVLVLIRESRPDAISARLGQETGGQGQQRLIEYGIGAQ
                     ILLDLGVREMVLLSNSPSRKIVGLQGYGLAVVGHRSLE"
     gene            complement(3219..3824)
                     /locus_tag="VFY74_06850"
     CDS             complement(3219..3824)
                     /locus_tag="VFY74_06850"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5931452.1"
                     /translation="MFTGLVSGVGILRERHGGRFVVACPYKRRSLEEGASIACDGCCL
                     TIVDIAKAKGEGARFAVEASNETLARTTLGSWREGRRINLERALALGDELGGHLLTGH
                     IDGTATILAREPDGGSVRFVLQSLTEHAPFIAAKGSVALDGVSLTVNDVAGARFGVNI
                     IPYTLAHTTWGDRRPGDLVNLEVDLMARYLARLREQERLAP"
     gene            complement(3797..4930)
                     /gene="ribD"
                     /locus_tag="VFY74_06855"
     CDS             complement(3797..4930)
                     /gene="ribD"
                     /locus_tag="VFY74_06855"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5931453.1"
                     /translation="MSLSDEDHMAQALQLARRGFGQTWPNPSVGAVVVDPTGEVLARG
                     WTAPGGRPHAEAIALERAGGKARGATLYITLEPCAHQGGRGPACTEIIAAARPARAVI
                     ALRDPDPRTQGRGIERLAASGIEVKEGVLSEEAKAVTLGHILRVTEERPAVTLKLAVG
                     ADGLIPQGNGAPVWATGPDARAHGHLLRARHDAILVGRGTILADDPTLTCRLPGMSCR
                     SPVRVVLDRRLRTPPGARLFDDLMVPVWLVCSADEEHPNTDALQDRGAEIVGVPVDDQ
                     GLLALRDALETLAARGLTRVLVEGGPSVARAFLDADLIDEVVVYRGPKPVGPGGLLPF
                     VGDGLDRVTASGHFTEIEARSFGPDRMSWWRRVGRCSPALSAA"
     gene            complement(4953..5453)
                     /gene="nrdR"
                     /locus_tag="VFY74_06860"
     CDS             complement(4953..5453)
                     /gene="nrdR"
                     /locus_tag="VFY74_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX5931454.1"
                     /translation="MRCPYCTSTNTQVKDSRPSEDHSTIRRRRVCADCGGRFTTFERV
                     QLRELTVVKRSGRRMPFDRDKLMRSVQIALRKRPVDPERVERMVSGIVRRLENMGEND
                     IRSETIGRLVMEALKALDDVAYVRFASVYKNFREAKDFEALLGELAATEDDDAGDGKA
                     AAPIED"
     gene            complement(5494..6801)
                     /gene="glyA"
                     /locus_tag="VFY74_06865"
     CDS             complement(5494..6801)
                     /gene="glyA"
                     /locus_tag="VFY74_06865"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5931455.1"
                     /translation="MTASDARRAEARGNAFFTKHLEETDPEIWDAIEDEFDRQQHEIE
                     LIASENIVSQSVLDAAGTLLTNKYAEGYPGRRYYGGCQYVDVVEQLAIDRAKRLFSCA
                     FANVQPHSGSQANQSVMLALIKPGDTVLGMSLAHGGHLTHGAPPNLSGKWFNAVQYGV
                     TKTDNRIDYDEVERLAKEHKPALIIAGGSAYPRIIDFARFRKIADSVGAFLMVDMAHF
                     AGLVAAGLHPSPLPHAHVVTTTTHKTLRGPRAGLVLSNDEEIGKKINSAVFPGLQGGP
                     LMHIIAAKAVAFGEALRPEYKIYIKGVVDNAKALGETLVKGGLALVSGGTDTHLMLVD
                     LRPKKLTGKAAEIALGRANITCNKNGVPFDPEKPAVTSGIRLGSPAGTTRGFGVAEFQ
                     QIGGMIIEVLDGLAAKGDDGNATVEASVKKRAIALCERYPIYR"
     gene            complement(6984..8315)
                     /locus_tag="VFY74_06870"
     CDS             complement(6984..8315)
                     /locus_tag="VFY74_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5931456.1"
                     /translation="MPATAGPFSWLTGKNQQQQQEQQLQQQPFPQQQQPGRNPGFFPF
                     FGGGRANRQGGGPDPTTAPRADELPRDDPEAYLITNPALGSPTLAARNIEATKAAIER
                     YRGIVAQGGWPTVPAKVMRPGQRGQEIEILQRRLEVTGDLFGQSVPGEFDAAVVVAVK
                     KFQARHGLPATGIIDSKATIQALNMSAYIRLAQLQTSLKRLQTIAPSTGGRYVVVNIP
                     AAQVEAVAGGEVVSRHAAVVGKPERATPEISSKIQEINFNPYWYVPRTIIHKDLIPKG
                     REFARRGQDMLASYRMQAFDAAGNPLDPRQIDWNSPAVYNYNFRQLPWEENSLGFVKI
                     NFPNKDSVYMHDTPLKSLFGRAVRFESSGCVRVHNVETLVAWILRDTPGWSYDRIVSM
                     KQTGEQIDVKVAKPVPVYLAYISAWGTPDGQVNFRPDIYGLDGASTTASAY"
     gene            complement(8674..9159)
                     /locus_tag="VFY74_06875"
     CDS             complement(8674..9159)
                     /locus_tag="VFY74_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX5931457.1"
                     /translation="MQESVGEAEDLKNRYLVSLKLIERLHRLLLDVIKDEFERLGRSD
                     VNSVQALLLYNIGDAELTAGELTSRGYYLGSNVSYNLKKLVDAGYINHQRSTVDRRSV
                     RVKLTQKGQDVCSVVNALYQRQLKSLEQVGGIATDDLEVLNKALIRLERFWTDQIRYQ
                     L"
     gene            complement(9334..10404)
                     /locus_tag="VFY74_06880"
     CDS             complement(9334..10404)
                     /locus_tag="VFY74_06880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5931458.1"
                     /translation="MSDALGITFEENDRAGVVMLDRPARLNALTREMFEALSTQYRAW
                     APAPHIYGVVMQSSHPTVFSSGGDLKTLHEWIEQDAQGSIREFYRAAYSHVWVLEKFI
                     RPNVPLINGLVLGGGIGISLYGTHCVAGEGYRFGMPQVGIGFLPDIGGTYFLPRLLDW
                     TGLYLALTGRIIGPAEAYRQRLVSHTIPAAHFGVIKDALSDNHPIDRLLDGLHRDPGE
                     GELVRHTPLIDRAFSAPSVEGILERLDAAQDEDQAFAQETAAAIRAKSPTALKIAFSQ
                     YRRGKSLDLAAALKLEFRLASHLIGSHDYREGIAARIAGKGRQPDWQPSTLAKVEDAA
                     VDRLFTTPAEDELVLANLTPGL"
     gene            10546..11652
                     /locus_tag="VFY74_06885"
     CDS             10546..11652
                     /locus_tag="VFY74_06885"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX5931459.1"
                     /translation="MTPNLARLSLVIFLALPIGADAETVDLPPLPKRNPLRGPVVTKP
                     VLPGDQPTVPWTEAEIADAVAKCKALLTGDPLDYEFLPPIKEGLCGTPAPVRLRAVGH
                     DPKVTINPPATLSCPMADALGEWLRDTVQPEAKAVFGSAVVKIQNSSSYVCRNRYGGA
                     GKPLSEHALANAFDVSEFVLANGVRISVLDHWPRVVTYPPARPESSDRDRVVTAAKDE
                     PPPVPQPARASKSNPFRLDGGAASAPPAESKGRDRDDVVAAAKAEPDEPTADAEPALD
                     PNPFDDHAALAPPAEPEGGDREGGVDAAKPDPGERPPVTTEPGQESKSKFVTYLHEDA
                     CRRFGTVLGPNANDAHKDHFHLDMKKRRRASFCE"
     gene            11860..12822
                     /gene="hemB"
                     /locus_tag="VFY74_06890"
     CDS             11860..12822
                     /gene="hemB"
                     /locus_tag="VFY74_06890"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5931460.1"
                     /translation="MRRNRRADWSRRLVRESSLASSDLIWPIFLIEGTGDRAPVASMP
                     GVERLSIDLAVKAAKEAAALGIPAIAVFPNTDPSLRDEAASEAVNPGNLVCRAVRAIK
                     AAVPEIGIICDVALDPYTSHGHDGLLIAGEIDNDRTVELLIQQALVQAEAGCDILAPS
                     DMMDGRIGAIRAGLEANGFANTQLLAYAAKYASAFYGPFRDAAGSGARLKGDKLSYQM
                     DPANGDEALREVALDIAEGADMVMVKPGLPYLDIVWRVKQAFGVPTFVYQVSGEYAAL
                     AAAAERGWLDRERVILESLMAFKRAGADAIFTYFAPEAAKLLDR"
BASE COUNT         2251 a         4124 c         4204 g         2298 t
ORIGIN      
        1 gcccgaaacc cccgcgcatg gcgtcacgcc cgcggcaaaa tgccaaagtt gcaggcgcgg
       61 cgcaacagag gcttgtccac cacgtttcgg cctaaggcgc gctcggcttg agcctagaaa
      121 tgggcatagc cgcgaacgtc aagcgcaagg ctcttcccgt cggcgccaag cacgaggagc
      181 ccggcttccg ggctctccag gcgaccgatc ggggtcacgg gcgttcccct ggctgtcgcc
      241 tcggcctcga aagcggcagc gctcccttga gggaccgcag ctacgatctc atagtcgtct
      301 cccgccgcga gaagagccgc caacaattgc ggctcgcgct tgagagcttt ctcccctgcc
      361 gaagagaacg gcacgagccc ggcctcgatc acggcgccga catgagagac gctcgcgagc
      421 ttgccgatat cgcccacgag cccatccgac acatcgatcg caccttgggc gaaattgcgc
      481 atgacaattg cgagttcgct gtgcggcaga gggcggcgat agcgctcgat gacgaaggcg
      541 aggtcctctt cggtgaaacc ccaatcgttg gcaagcgccg gctctcggag ggcgcgaagg
      601 ccgagataag cgtcgccgat cgttcccgtc acgtagaggc ggtcgccccg tttggccccg
      661 agccggagca cggcatggcc ttggggaacg aggccgagcg cggtgatggt gatgctcaag
      721 gggccgggga tgcgcgtggt gtcgccgccg acgagcgcga tcccggtgcg ggtctgaagc
      781 gcgcgcaggc ccgaggcgaa ggcctcgagc caagcaggcg acggctcttc cgggagcgct
      841 agcgccagga gataggcgta agggcgcgcg cctttggcgg cgagatccga caggttcacc
      901 gccaaggcct tatgcgcgat gctgtcgggc ggatcgcctt ttagaaaatg cacgccctcg
      961 acgatcgtgt cgcaagagat cacgatctcc tggctctcgg gcggcctgag gacggcggcg
     1021 tcgtcgcgca agccgagcgc tgaggcatcg gtggcaaggg gcgcgaaata gcgggcgatg
     1081 atgtcgaact cgccaagccg ctgcccctct gcgcgctcgc cccggtcaac catccctgct
     1141 tcctccggcg gcaaattctt gcggccgcgc tctgtgcccg aggcgatcga gaatgccgtt
     1201 gaccgccttc ggctcctcct cggcgaagaa ggcgtgggcg atgtccacat attcggaaat
     1261 gaccacgcgc actggcacgt cgccgcgcac caggatttca taggcgcctg cgcgaaggat
     1321 ggcgcggagg atcgaatcca cgcgggtcaa tcgccagccc tcggcgagct gcttgtcgat
     1381 caagggatcg atacgaagct gctcgcgcac aacgccggcg acgaggtcgc ggaagaagcc
     1441 ggactccgct tcagcgtatt gccagccttc gatctcctga cccaagcgat gggactcgaa
     1501 ttcggcgatc acctggttga ggtcgatccc ggtcatgtcc atctggtaca gggcctggac
     1561 cgcggcaagg cgggcggcgc tgcgggagga ggcgcggggg gtgcttcggc tctccttcgc
     1621 catggcggct agcgccgttt cgccgaagag aagtgctgtt cgagttgcat gagagtgagg
     1681 caggcgcgcg ccgcgtcgcg gcccttgttg cgcccatcga cgcttgcccg ctcccaggct
     1741 tgctcgcggc tgtcacaggt caaaatgccg aacccgatcg gcacggcatg ctgaacggcg
     1801 agctgatgca gccccgccgc cgattcccgt gccacaatat cgtaatgggt cgtctcgccg
     1861 cggatgacgc agccgagcgc cacgcagccg cgatgccgcc cggcccggcc cacgaaaccg
     1921 ttggcgagtg cgagactgag cgcaagcgca atctcgagcg aacccgggac gctaatccgc
     1981 tcgtaagcca tgcgatgggc ttcgagctcc tcgattgcgc cgcgggcaag ctcgtcggca
     2041 atgtcctcgt agaagcgtgc ctcgatgatg aggacggagt gcgggccttt gcgcgtcatc
     2101 gcgttactcc aggctccggt ggccgaccac cgcaagccca taaccttgca gacccacgat
     2161 cttgcgcgaa ggagaattgc tcaggagcac catctcgcgc acgccgagat cgagcaggat
     2221 ctgggcgcca atgccgtatt cgatgagccg ctgctggccc tggccccccg tttcctgccc
     2281 caggcgtgca gaaatcgcat caggccgcga ctcgcggatc agcacgagca cgccgcgtcc
     2341 ttcgcgcccg atgatgtcca tggccttgtg cacgagggtc tcgcggtggg tcccgatcga
     2401 aatcacgtcg tccattgtgt tgagcgcatg cacccgcacg agcacgggcc cgcctgaggc
     2461 gacgtcgccc ttgatgagcg cgacatgctc ggccgggtcc acggtggtgg catagacctt
     2521 gaggtcgaac tgcccgccga aggcgctctc gaccgtggtc tcggccacga ccttgaccag
     2581 atgatcgtag cggagccgat aggcgatcag gtccgcgatg gccgcgatct tcagtccatg
     2641 gcgatcggcg aaggcgatca ggtccggaag ccgcgccatg gtgccgtcat cattcatgat
     2701 ctcgcagatc acccctgccg gataaaggcc ggcaagccgg gcgagatcca ccgccgcttc
     2761 ggtatggccc gcgcgcacga gcacgccacc atctttggcc accagcggga acacatgtcc
     2821 cggggtcatg aggtcgcgat agtccttggt ggggtcgatg gcgacggaga cggtgtggga
     2881 gcggtcatga gcggagatgc cggtcgagac cccttcccgc gcctcgatcg acacggtgaa
     2941 ggcggtcgag tggcgggcgc gattggagcg cgacatcaac tccaggtgaa gctgcttggc
     3001 gcgttcgggc ggaagtgcaa gacagatcaa gccgcggcca tgcttggcca tgaagttgat
     3061 ggcgtcgggc gtggccattt gcgccggaat gaccagatcg ccctcgttct cacgctcctc
     3121 ggcatcgacc agaatgaaca tgcggccgtt gcgcgcgtcc tcgaccacct cctcgattgg
     3181 ggagaggaat tgatggtgcc gggtcttggg cgcaatcgtc acggggcaag cctttcctgc
     3241 tctctgagac gtgcgaggta gcgcgccatg agatcgactt caaggtttac caaatcgccc
     3301 gggcgccggt ctccccatgt cgtgtgggcg agcgtgtagg gaatgatgtt caccccgaac
     3361 cgcgcgccgg cgacgtcgtt aacggtcagc gagacgccgt cgagcgcgac cgatcccttg
     3421 gcggcaatga agggagcgtg ctcggtgagg ctttgcagca cgaagcggac gctgccgcca
     3481 tcgggctcgc gtgcgaggat cgtggccgtc ccgtcgatat ggccggtcag gagatggccg
     3541 ccgagctcat cgccgagcgc aagcgcgcgt tcgagattga tgcggcgtcc ctcccgccag
     3601 gagccgagcg tggtgcgcgc gagcgtctcg ttggaggcct cgacggcaaa gcgcgcgccc
     3661 tcgcccttcg ccttggcaat atcgacgatg gtcagacagc aaccgtcgca ggcgatcgaa
     3721 gccccctctt caaggctccg gcgcttgtac gggcaggcga ccacgaaacg cccgccgtga
     3781 cgctcgcgga ggattcctac gccgctgaca aggccggtga acatcgccct actcgccgcc
     3841 accaactcat ccggtcgggc ccgaacgagc gggcctcgat ctcggtgaaa tgcccgcttg
     3901 cggtcacgcg atcaagtccg tcgccaacga aaggcagaag accgcccggt cccacgggct
     3961 tcggaccccg atagacgacg acctcgtcga taagatcggc atcgagaaag gcacgcgcca
     4021 cggaaggccc gccctcgacc agcacgcgcg tgagcccgcg ggccgcaagc gtctcgagcg
     4081 cgtcgcgcag ggcaaggagg ccttgatcgt cgacgggaac gccgacgatc tcggccccgc
     4141 gatcctggag ggcatccgtg ttcggatgct cttcgtcggc ggagcagacg agccataccg
     4201 gcaccatgag atcgtcgaac agtcgcgctc cgggcggcgt ccgcaggcgc cggtccagca
     4261 ccacgcgcac cggagagcgg caggacatgc cgggaaggcg gcacgtcaat gtcgggtcgt
     4321 cggcgagaat ggtgccgcgg ccgacgagga tcgcatcgtg ccgcgccctt aagagatgtc
     4381 cgtgggcccg cgcgtcagga ccggtcgccc acaccggggc gccattccct tgcggtatga
     4441 gcccgtcggc gccgacggcg agctttagcg tcacggcggg acgctcctct gtgacgcgaa
     4501 ggatgtgacc gagggtgacg gccttcgctt cctcgctcag cacgccctcc ttcacctcga
     4561 tgccggaggc ggcaaggcgt tcgatgccgc ggccttgtgt gcgcggatcg ggatcgcgaa
     4621 gcgcgatcac ggcccgcgcc ggccttgccg ccgcgatgat ctcggtgcaa gccggacccc
     4681 tccctccttg gtgggcgcag ggctccagcg tgatgtagag cgtggcgccc cgcgctttcc
     4741 cgccggcccg ctccagcgcg atggcctcgg cgtgaggccg gcctcccggc gccgtccagc
     4801 cgcgggcgag aacttcgccg gtcgggtcaa cgacgaccgc gcccacggaa gggttcggcc
     4861 aggtctggcc gaacccccgc cgggcaagct ggagggcctg cgccatatga tcctcgtcgc
     4921 tcaggctcat cgtcttgatg tggggtcgaa gcctagtctt cgataggggc ggccgccttg
     4981 ccgtcgcccg cgtcgtcgtc ctcggtcgca gcgagctcgc ccagcagcgc ctcgaaatcc
     5041 ttggcctcgc ggaaattctt gtacactgag gcaaagcgta cataagcaac gtcgtcgagc
     5101 gccttgagcg cctccatcac caggcgcccg atggtttcgg acctgatgtc gttctcgccc
     5161 atattctcca ggcgccgcac gatgccgctc accatgcgct cgacccgctc ggggtccacg
     5221 gggcgcttcc tgagcgcgat ctgcaccgac cgcatcagct tgtcgcggtc gaacggcatc
     5281 cgccgcccgc tccgcttgac gaccgtcagc tcgcgcagct gcacccgctc gaaggtggta
     5341 aagcggccgc cgcaatcggc gcagacgcgg cgccgcctga tcgtgctatg atcttcgctc
     5401 gggcgcgagt ccttcacttg cgtgttggtg ctagtgcaat aagggcatcg catcggcgac
     5461 ggccctcccc ttttcagtca cctcacgcga gacttaacgg taaatcggat agcgctcgca
     5521 gagtgcgatg gcccgcttct tcacgcttgc ctccacggtg gcgttgccgt cgtcgccctt
     5581 ggctgcgagc ccgtccaaaa cctcgatgat catcccgccg atctgctgga actccgcgac
     5641 gccgaatccg cgcgtggtcc cggccgggga cccgagcctg atgcctgacg tgaccgccgg
     5701 cttctccgga tcgaatggca cgccgttctt gttgcaggtg atgttggccc ggccgagcgc
     5761 gatctcggcc gccttgccgg tgagcttctt cggcctgaga tcgaccagca tcagatgcgt
     5821 gtcggtgccg ccggagacga gtgccaaccc acccttgacc agggtctcgc cgagcgcctt
     5881 ggcgttgtcg accacgccct tgatgtagat cttgtactcg gggcgaagcg cttccccgaa
     5941 ggccaccgcc ttggcggcga tgatatgcat cagcgggccg ccctgaagac cggggaacac
     6001 cgcggagttg atcttcttgc cgatctcctc gtcattggag agcacgagcc ccgcgcgcgg
     6061 tccgcgcagt gtcttgtgcg tggtggtggt gacgacatgg gcgtgcggca gcgggcttgg
     6121 atggagccct gcggcgacaa gcccggcgaa atgcgccatg tcgaccatca aaaaggcgcc
     6181 gacggaatca gcgatcttcc ggaagcgagc gaaatcgatg atgcgcgggt aggccgagcc
     6241 gccggcaatg atgagagccg gcttgtgctc cttggcgagc cgctcgacct cgtcgtagtc
     6301 gatgcgattg tcggtcttgg tcacgccata ctgcacggcg ttgaaccact tgccggacag
     6361 attgggcggg gccccatgcg tcaagtggcc gccatgcgcg agcgacatgc cgaggaccgt
     6421 gtcgcccggc ttgatgagcg cgagcatcac cgactgattg gcctgcgagc cggaatgcgg
     6481 ctgaacgttg gcgaaggcgc agctgaacag tcgcttggcg cggtcgatgg caagctgctc
     6541 gaccacgtcg acatattggc agccgccgta atagcgccgg cccggatacc cttccgcgta
     6601 tttgttggtg agcagcgtgc cggcggcgtc gagcacggac tgcgaaacga tgttctccga
     6661 agcgatcagc tcgatctcgt gctgctgacg gtcgaattcg tcctcgatcg cgtcccatat
     6721 ttcggggtcg gtttcttcca gatgcttggt gaagaaggcg ttcccgcgcg cctcggcgcg
     6781 tcttgcatca cttgccgtca tggctgactg ctcctcgagc tcacataccc cgcccgggaa
     6841 accacgcgca gcgacttttg ccgcgcgctc tcttcctcga gcgtttcgtt ggctctacca
     6901 catgtcgcgc ggaggcgcca gcaacacgca aggcgtgggc ggtcaagcgc tgcccgcgca
     6961 cggtgcgcga agtgccatgt cgatcaatag gctgaagcgg tcgtggatgc cccgtcgagg
     7021 ccgtagatgt caggccggaa attcacctgg ccgtcgggcg tcccccaggc gctgatgtaa
     7081 gcgagataga ccggaaccgg ctttgcgacc ttgacgtcaa tctgctctcc ggtctgcttc
     7141 atcgaaacga tgcgatcgta gctccagccc ggcgtgtcgc gcaaaatcca agcaaccagc
     7201 gtctcgacat tgtgcacgcg cacgcagcca gagctttcga accgcacggc gcggccgaac
     7261 aggctcttga gcggcgtgtc gtgcatgtag acgctgtcct tgttcgggaa gttgatcttc
     7321 acgaagccga gagagttctc ctcccaggga agctgccgga aattgtagtt gtagaccgcg
     7381 ggactgttcc aatcgatctg gcggggatcg agcggattgc cggcggcgtc gaaggcctgc
     7441 atgcggtagg aggccagcat gtcttggcct ctgcgggcga attcgcggcc cttcgggatc
     7501 aagtccttat ggatgatcgt gcgcggcacg taccaataag gattgaaatt gatctcttga
     7561 atcttgctcg agatttcggg cgtcgccctt tcgggtttgc cgacaacagc cgcgtgacgc
     7621 gagacgacct cgccgcccgc gaccgcctcg acctgcgccg ccgggatgtt gaccacgaca
     7681 tagcgtccac cggtcgaggg ggcgatggtt tgcagccgct tcagactggt ttgaagctgc
     7741 gcgagccgga tataggcgga catgttcaag gcctggatcg tcgccttgct gtcgatgatg
     7801 ccggtcgcgg gtaggccatg gcgcgcttga aacttcttca cggcgacgac gaccgcggcg
     7861 tcaaactcgc ctgggacact ttggccgaag aggtcgccgg tgacctcgag ccgcctctgc
     7921 aagatttcga tctcttgacc gcgctggccc ggtctcatga ctttggcggg gacggtcggc
     7981 caccctccct gggccacgat ccccctgtag cgctcgatgg cggccttggt ggcctcgatg
     8041 ttgcgggcgg caagcgtggg cgagccgagc gcaggattgg tgatcagata ggcctcggga
     8101 tcgtcgcgcg ggagctcatc ggcgcgcggg gccgtggtcg gatcgggccc tccgccctgc
     8161 cgattggccc tgcccccgcc gaagaaggga aagaacccgg gattgcggcc cggttgctgc
     8221 tgctgcggga atggttgttg ctggagttgc tgttcctgct gctgctgttg attcttgccg
     8281 gtcagccagg agaacggacc ggccgtagca ggcacggcct ctatcgcgac aagcgataca
     8341 gcgagactta tcgcagcgag gaacgccccg cgtttgctac tctgcgattt cagcattcgc
     8401 cgctccccgt cacttgcggc ttcatctcgc gcgaatataa gcggtttttc cttaacactc
     8461 tagttccaat gacctaacga ggcgtcatga tccgctgcgc cgccacgacg gatgcgcgcg
     8521 tgtgacgcct gctttccctt cgcaggtcga aatgatgtct agcgcccgaa tggggcgaat
     8581 ttgcgctgaa aagaccgctg ccttccaaag gcttgcgggc acggcggcag gtagttcccc
     8641 cgcggcgcta gagttttggc gcttcgagga agattagagc tggtagcgga tctggtcggt
     8701 ccagaaacgc tccagacgaa ttagggcttt gttgagcacc tcgagatcgt cggtcgcgat
     8761 gccgcccacc tgctcgagcg atttgagctg gcgctgatac agcgcgttca ccacgctgca
     8821 gacgtcttga cccttttgcg tgagcttgac ccgcaccgaa cggcggtcga ccgttgagcg
     8881 ctgatgattg atgtagccgg cgtcgacgag cttcttgaga ttgtacgaga cgttcgaacc
     8941 gagataatag ccgcggctcg tgagctcgcc ggctgtcagc tcggcatcgc cgatattgta
     9001 aagcaacagt gcctgcacgc tgttgacgtc cgaacggccc aagcgttcga actcgtcttt
     9061 gatcacgtca agcagcagtc gatgcagccg ctcgatcagc ttcaacgaaa cgagatagcg
     9121 attcttgagg tcttctgcct cgccgacgct ctcttgcatc cctatattca gggccacagt
     9181 catcgcctac ccttcgttag ttgttgttaa atccccaact tgacgatgac aatagacgcg
     9241 gcaacctaaa atctgcttaa gcgagacgct taagattgcg tgaaattcta gtatgaagtt
     9301 atgacgatgt tggctcgcct tttggcgagg ccgtcaaaga cccggcgtca gatttgccag
     9361 gacgagttcg tcctccgccg gcgtggtaaa caggcgatcg accgccgcat cctcaacctt
     9421 agcgagcgtt gacggttgcc aatcgggttg gcggcctttg cccgcgatcc gcgccgcgat
     9481 cccttcccga tagtcgtgac tgccgatgag gtgggaggcc aggcggaact cgagcttcag
     9541 cgctgcggcg aggtcgaggg actttccgcg ccgatattga ctgaaggcga ttttgagcgc
     9601 ggtcggcgac ttcgcgcgga tcgcggcggc agtctcttga gcgaaggctt ggtcttcgtc
     9661 ctgcgccgca tcgagccgtt cgagtattcc ctccacggac ggcgcggaga aggcgcgatc
     9721 aatcagcggc gtgtgacgca cgagctcgcc ttcgccggga tcgcgatgga gtccgtcgag
     9781 cagccggtcg atgggatggt tgtcgctcag cgcgtctttg atcacgccga aatgcgcggc
     9841 cggaatggtg tggctcacga ggcgttggcg ataggcttcg gccggtccga tgatgcgtcc
     9901 ggtcaaggcg aggtagaggc cggtccaatc gagaagccgc ggaagaaaat acgtgccgcc
     9961 aatatcgggc aggaacccga tgccgacttg cggcatgccg aagcgatatc cctcgcccgc
    10021 cacgcaatgc gtgccgtaaa gactgatgcc gatgccgcct ccgagcacga gcccgttgat
    10081 cagcggcacg ttcggcctga tgaacttttc gaggacccag acatggctgt atgccgcccg
    10141 gtagaactcc cggatcgatc cttgcgcgtc ttgctcgatc cactcatgca atgtcttgag
    10201 atcgcccccg ctcgagaaca cggtcgggtg cgacgactgc atcacgacac cgtagatgtg
    10261 gggcgcgggc gcccaagcgc gatactgcgt gctcagggcc tcgaacatct cccgcgtcaa
    10321 ggcattgagc cgcgcgggac gatcaagcat caccaccccg gcgcggtcgt tttcctcgaa
    10381 ggtgatcccc agcgcatcgg acaaattgcg gtgtctcctc ccgctaaatc gttgtaatga
    10441 ccccgaacca agcgcctcga tcgggcgtta tacaaaggct cgcggcagct agcactcaat
    10501 ccccgaaatc acaaggcttg caggaaaggc aatcgattgc cggacatgac gcctaacctt
    10561 gctcggctca gtctcgtaat cttcttggcg cttccgattg gcgccgacgc ggaaacggtc
    10621 gaccttccgc cgttgccgaa gcggaacccc ttgcggggac cggtggtcac aaagcccgtt
    10681 ctgccgggcg atcagcccac cgtgccgtgg accgaggccg agatcgccga cgccgtcgcg
    10741 aagtgcaagg cgcttctgac cggagatccg ctcgactatg agttcctgcc gccgatcaag
    10801 gaaggtttgt gcgggacacc ggcgccggtc cggctgcgtg ccgtgggcca cgatccgaaa
    10861 gtaacaatca atccgccggc gacgctcagc tgtcccatgg ccgatgcttt gggggaatgg
    10921 ctgcgcgata ccgtccagcc ggaagccaag gcggtgttcg gaagcgccgt ggtgaagatt
    10981 cagaattcca gctcctatgt gtgccgcaac cgctacggtg gcgcgggcaa gccgttgagc
    11041 gagcacgcgc tcgccaatgc gttcgacgtg tcagaattcg tgctcgccaa tggcgtccgt
    11101 atcagcgtgc tggatcattg gccgcgcgtc gtaacgtacc cgcctgccag acccgagagc
    11161 agtgatcgcg accgcgtcgt caccgcggcg aaggacgagc cgccgccggt tccgcaaccg
    11221 gcgcgggcct ccaagtccaa cccattccgc ctggacggcg gcgcggcttc cgcccctcct
    11281 gccgaatcca agggtcgcga tcgtgacgac gtcgttgccg cggcgaaggc cgagccggac
    11341 gagccaacgg cggatgcgga accggccctc gatcccaacc cattcgacga ccacgcggct
    11401 ctcgcccctc ctgccgagcc tgagggcggc gatcgcgagg gcggcgttga cgcggcgaag
    11461 cccgatccgg gcgagcggcc gccggttacg acggaaccag ggcaagaatc caagtcaaaa
    11521 ttcgtcacct atttgcacga ggacgcctgc cgccgtttcg gcacggtgct cggtccgaac
    11581 gccaatgacg cgcacaagga ccactttcat ctcgacatga agaaacggcg gcgggcgagc
    11641 ttctgcgaat agggctcgcg cagggcaaac catgtttaac gagagacatt ggcacccgcg
    11701 aagcgctgat ggtagtttgc ccgcatcttg acagcgagcg gcggaagagc gcggatgacg
    11761 gctcataaag acaaaccggt ctcattgcgg agcgaacggg cgaaacgcgc gggtgtggcg
    11821 tcggagcagg tcaaaggacc gggctttccg gcgacgcgga tgcggcgcaa ccgccgcgcc
    11881 gactggtcgc gccggctggt ccgcgagagc tcgctcgcaa gctccgacct catctggccg
    11941 attttcctga ttgaagggac cggggaccgc gctcccgtcg catccatgcc gggcgttgag
    12001 cggctatcga tcgatcttgc cgtcaaggcc gccaaagagg ctgcggcgct cggcatcccg
    12061 gccatcgcgg tcttccccaa taccgatccc tccttgcgag acgaggctgc gagcgaggcg
    12121 gtcaatcccg gcaatctcgt ctgcagggcc gtgcgcgcga tcaaggcggc ggtgcccgag
    12181 atcggcatca tctgcgacgt ggcgctcgac ccttacacca gccacggtca cgacgggctc
    12241 ttgatcgccg gcgagatcga caatgaccgc accgtcgagc tcttgatcca gcaggccctc
    12301 gtccaggccg aggcgggctg cgacatcctc gccccttccg acatgatgga tggcaggatc
    12361 ggtgcaatcc gcgcgggtct tgaggcgaac ggctttgcca acacgcagct ccttgcttat
    12421 gcggcgaaat atgcgtcggc cttctatggg ccgttccgcg atgcggccgg ttcaggcgcg
    12481 agactcaaag gcgacaagct gagctatcag atggaccccg ccaacggcga cgaggcgctc
    12541 cgcgaagtcg cgctcgacat cgccgagggc gcggacatgg tgatggtgaa gccggggctc
    12601 ccttatctcg acatcgtttg gcgggtgaag caggcctttg gcgttccgac cttcgtctac
    12661 caggtctcgg gcgagtatgc ggcgcttgcg gccgcagcgg agcgtggctg gctcgaccgg
    12721 gagcgcgtga tcttggagag cttgatggcg ttcaagcggg ccggcgccga cgcgatcttc
    12781 acctatttcg cgccagaggc ggcgaagctg ttagaccgct aagggcgtag ttctcgattt
    12841 tatgaacggc cgggatcggc tgcaagaggc gcgggct
//
LOCUS       DASUQP010000156         9145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_214809,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000156 DASUQP010000000
VERSION     DASUQP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214809"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..626
                     /locus_tag="VFY74_06895"
     CDS             243..626
                     /locus_tag="VFY74_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HEX5931461.1"
                     /translation="MQLVATLDEYGKPAWITAMVGGFLLWWPIGLGILAYLIWSGRMG
                     CCGNRVAWRQYFTDEARRFGGGMTAFGSTGNRAFDEYRAETLRRLEEEANEFQAFLQR
                     LRHAKDKAEFDQFMAERGGAAPSAS"
     gene            complement(820..4542)
                     /locus_tag="VFY74_06900"
     CDS             complement(820..4542)
                     /locus_tag="VFY74_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5931462.1"
                     /translation="MSGGGTWQFFIDRGGTFTDIVARAPDGRLLTRKLLSENPEAYAD
                     AALAGIADLIGVPRNRPLPSQAIASIRMGTTVATNALLERKGDPLLLIVSQGFRDALE
                     IGYQARPKIFARRIEKPSMLYARVVEVPERLRADGGVEVPLDRDATRAALRAARADGI
                     SAVAIAFMHSYAFPAHEREAARLAREERFTQVSASHEVSPLVKFVGRGDTAVVDAYLS
                     PLLRRYVDRVARALKPSSSLPLMGRVARLKAEPGGVRRQTAPHPAGFVGRPPHEGEAK
                     APRLLFMQSSGGLTSAGLFRGKDAILSGPAGGVVGAVETAQAAGFAKVIGFDMGGTST
                     DVCHYDGAYERSFESQVAGVRVRAPMMLIHTVAAGGGSILHYDETRFRVGPDSAGADP
                     GPLCYRRNGPLTVTDANVMTGKLTPDFFPRIFGPRQDQPLDAESVLKAFKSLARKLGL
                     ARAPEEIADGFIKIAVENMANAIKTISVQRGYDVTEYVLNSFGGAGGQHACLVADALG
                     IQSVLIHPLSGVLSAFGMGLAALSATRTKTVLARLDEEGLAALAKIRAGLEEEVRQEL
                     LRQGVDGGAIAITAQAHLRYAGTDSALPIPVASLRGMRALFEIAHEKRFGFISPEKDI
                     EIEAVEVEAKGGGAAIAEPALTPETPAPAAPDAEARIFTAADWHDAPVFLRASLGRGQ
                     SIPGPALIIEDHQTVVVEPGWQAHVTARNDLLLTRVAKKVEARVGRRADPVMVEVFNN
                     LFVSIAEQMGYALQNTARSVNIKERLDFSCAIFDAKGNLIANAPHIPVHLGSMDRSVA
                     TVLREVGEKLKPGDVYMLNAPYNGGTHLPDITVITPVFGENSPDLLFFVAARGHHADI
                     GGIAPGSMSPRATRIEEEGVYIDPFKLVARGRFREEEALALLTKAPYPARNPAQNIAD
                     LKAQVAANEKGAAELRAMVRHYGLPVVRAYMRHVQDNAAEAVARVIAKLKSSRFEVET
                     DQGSRIKVAIKIDRKARKATVDFTGTSAQTSDAFNAPEPITRACVLYVFRTLVDDDIP
                     LNAGCLRPIKIVVPKGSMLKPRYPAPVAAGNVETSQTIVNCLYGALTCLGSAQGTMNN
                     LTFGNESYQYYETICSGAPAGPGFDGAPAVQTHMTNSRLTDPEVLELRYPVLVERFEI
                     VRGSGGKGKWRAGDGTLRAIRFLARMDCAVLSGFRRLRPFGLLGGEEGQAGENWVRRK
                     AGRLERLKGADQTVLEPGEAIIIKTPTGGGFGDRRT"
     gene            complement(4539..5069)
                     /locus_tag="VFY74_06905"
     CDS             complement(4539..5069)
                     /locus_tag="VFY74_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018446347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5931463.1"
                     /translation="MAVYELEGTRPEFPADGLYWIAETAAVIGRVRLHSDVSIWYGSV
                     LRGDNEWIEIGARSQVQDNATLHTDPGFPLTIGEDCVIGHNVILHGCTIGDASLIGMG
                     AIVLNGAKIGSNCLVGAGALITKGKAFPDDTVILGAPAKSVRASDDAARAMIARATEV
                     YVKRWKRYAAELKRIG"
     gene            complement(5081..6889)
                     /locus_tag="VFY74_06910"
     CDS             complement(5081..6889)
                     /locus_tag="VFY74_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931464.1"
                     /translation="MTAGRSLLIALTLSGLVMIFPDLLRVAEPLASIGLFADGDGLIT
                     DVTEPFPDEAASPAWRAGIRVGDRLDLEAMRCRLGALEKCSSAIATLGGVQYLLPGRT
                     VTLDLVAADGQPARHVSLVAEPPPSNALVRGVNFLCQIAGILVVLAAAWLVWTRPSAM
                     SWGFFLYVNWFNPGQVHGFYALLEPRPLLFFVTDIAMVLAQAIGYAGLLLFVLRVPNN
                     ETEARWRGLERALPIVAVVFALLLFASYGSLFGYPVETMTRISILMGFLVAAGALAIL
                     MVRRRTQTPEDYQRVRWVIWGCLIGLPAFLLGELACATTIFHTRWGNFTPSDDVIGLL
                     YLVNGVLCLFVFEALRRPRVVNVTIPLRRVTILGLTLSVPFLLLHHEVERMQKYLNLP
                     GWAWIALGAAAIFLLTRLHEGAVHFADRYFNRAVDDAERRLGHAIRQAKKAGEIDRLL
                     ADDTFKALKLASAAAFRREGLAFRRDGNGKGWSRGTATSIKPDVPLLAPVSEGKPFSV
                     ADQDEDGIDLPTGLARPVLAVPAVNPVRCFAVSLYGPHESGTDLDPSERAMLARLGAQ
                     AAAMYAELENGVLRDEIARLERALRAAKPEAKSPAR"
     gene            7099..7416
                     /locus_tag="VFY74_06915"
     CDS             7099..7416
                     /locus_tag="VFY74_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931465.1"
                     /translation="MDTEAPKGTPRHGGNILTIFITSLCAAIVVAGGFGIVVQAAILA
                     AVARLGLGETVLWIGSAINAALTLWLALWTFARSWHVERRLKAGLEVDEPKLSILANF
                     RGS"
     gene            7520..8239
                     /locus_tag="VFY74_06920"
     CDS             7520..8239
                     /locus_tag="VFY74_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX5931466.1"
                     /translation="MSIWGKLAGAAAGLAVGGPIGALLGGVAGHYVIDRDKEEEGPAE
                     NQVAFTVGVIALGAKMAKADGVVTMDEVNAFKEVFKVPDGEMKNVARVFNLAKQDMAG
                     YEAYAEQLSTMFKGNRKVLEDVLEGLFHIAKADEALHPREEQFLGGVAKRFGMTDTEF
                     AYIKARHVAAAKRNPYDVLGIKPSISNEELKSHYRRLVAESHPDKLIARGVPKEFVVL
                     ATEKVATINEAYDQIAKERGM"
     gene            8363..9130
                     /locus_tag="VFY74_06925"
     CDS             8363..9130
                     /locus_tag="VFY74_06925"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5931467.1"
                     /translation="MTVAPDSPLATLWCPSPNREARRNGIKPDMLLLHYTGMEQAESA
                     LDWLTRQESGVSCHYFIDEEGRIAQLVLESERAWHSGQSLWAGETDLNSCSIGIEIHN
                     AGHDFDYPHFTEAEMRAVEALCRDILSRHAIPAHRVLAHSDVAPGRKRDPGEKFDWER
                     LARAGIGLWVPPTPLGDDTGLGPGDEGESVAALQRALLDFGYGVEVTSTYGTGLEKAV
                     EAFQRHFRPARIDGRADASTSDTLDRLLVARSRAAIV"
BASE COUNT         1654 a         2929 c         3000 g         1562 t
ORIGIN      
        1 gcgacggtag aacgccgttc ggtgaatcgc caaaccctca ccccctgcca cgctgcccca
       61 agcctagacc gcagaggatt agaatattct tgccattttt ggggccgcgg ggccgcggct
      121 ccgtcccgtc cacagcccgc aaacggcctc caatcacatt ttgtgtcggc ccttgactct
      181 ggacgggcga ggggcatcta tgtaaatgtg aataacattc acaccgtagg aagggaccca
      241 caatgcagtt ggttgcgacc ttggacgaat acgggaagcc cgcatggatc acagccatgg
      301 ttggcggctt ccttctctgg tggcccatcg gccttggcat tttagcttat ctgatctgga
      361 gtggacgcat gggatgctgt ggaaatcgag tcgcatggcg gcagtatttc acggatgagg
      421 cgcggcgctt tggcggaggc atgacggcct tcggctcgac cggaaaccgc gccttcgacg
      481 agtatcgtgc ggagacgttg cggcggctcg aggaggaggc gaacgagttt caggccttct
      541 tgcagcggct gcgtcacgcc aaggacaagg ccgagttcga ccagttcatg gccgagcgtg
      601 gcggcgctgc tccttcggcg tcgtagcgtg aaactcgtca ttccggcgta agccggaatc
      661 cagggagaca gacactggcg cacgcagtcc tggataccgg ctttcgccgg tatgacgagt
      721 cagggatttc ggacaagcgt agtagagagg caacgaaaga caagggcgtg gggccttggt
      781 tccacgccct tgttgttgac cggccgcccg cggtctcggt catgtgcgcc gatcgccgaa
      841 gccgccgccg gtcggcgtct tgatgatgat ggcctcgccg ggctcgagca cggtctggtc
      901 tgcgcctttc aaccgctcga gccttcccgc ctttcgcctg acccaattct cgccggcctg
      961 tccttcttcg ccgccgagga ggccaaaggg ccgcaacctt cggaagcccg agaggaccgc
     1021 gcaatccatc cgcgcgagga agcgaatggc gcggagcgtg ccgtcgcccg cgcgccattt
     1081 ccccttgccg ccggagccgc gcacgatctc gaagcgttcg acgagaacgg ggtagcgaag
     1141 ctcgagcacc tccgggtcgg tgaggcgcga attggtcatg tgcgtctgca cggccggcgc
     1201 cccatcgaaa ccgggtccgg ccggcgcgcc cgagcagatc gtctcgtaat actgatagct
     1261 ctcgttgccg aaggtgagat tgttcatcgt gccctgcgcc gagccgagac acgtcagcgc
     1321 gccatagaga cagttcacga ttgtctggct cgtctcgaca ttgcccgcag cgacaggcgc
     1381 cggatagcgc ggcttcaaca tcgagccttt cggcacgacg atcttgatcg ggcggaggca
     1441 gcccgcgttc agcggaatgt cgtcatcgac gagcgtgcgg aagacataga gcacgcaagc
     1501 cctggtgatc ggctcgggcg cgttgaacgc gtccgacgtc tgcgcgctcg tgccggtgaa
     1561 atcgacggtc gctttgcgtg ccttgcgatc gatcttgatc gcgaccttga tgcggcttcc
     1621 ctgatcggtc tcaacctcga agcgtgaact cttaagcttg gcgatgacgc gcgccacggc
     1681 ttccgcggcg ttgtcctgca catggcgcat gtaggctcgc accaccggca acccgtaatg
     1741 acggaccatg gcgcggagct ccgccgcgcc cttttcgttg gcggcaacct gcgccttgag
     1801 gtccgcgatg ttctgcgccg gattgcgcgc ggggtagggc gccttggtga gcagcgcaag
     1861 cgcctcctct tcgcggaagc gtccccgcgc gaccagcttg aacggatcga tatagacgcc
     1921 ttcctcctcg atgcgcgtcg cccgcgggct catcgagccg ggcgcgatcc cgccgatatc
     1981 ggcatgatgc ccgcgcgcgg cgacgaagaa gagaaggtcg gggctgttct cgccgaagac
     2041 cggcgtgatc acggtgatgt cggggagatg ggtgccgccg ttgtaaggcg cgttcagcat
     2101 atagacatcg ccgggcttga gcttctcgcc cacttcgcgc agcacggtgg cgacgcttcg
     2161 gtccatggag ccgagatgga ccgggatgtg cggcgcgttg gcgatgaggt tgcccttcgc
     2221 gtcgaagatg gcgcaggaga agtcgagccg ctccttgata ttgaccgagc gcgcggtgtt
     2281 ttgcagcgca tagcccatct gctcggcgat cgagacgaag agattgttga acacctcgac
     2341 catgaccgga tcggcgcgtc tgccgacccg tgcttccacc ttcttggcga cgcgggtgag
     2401 caagaggtcg ttacgcgccg tgacgtgcgc ttgccatccg ggctcgacca cgacggtctg
     2461 atggtcttcg atgatgagcg ccggccccgg gattgactgc ccccggccga gactggcgcg
     2521 caggaaaacc ggggcatcgt gccaatccgc ggcggtgaag atgcgcgctt ccgcatcagg
     2581 ggcggcgggc gcgggggtct caggcgtaag cgcaggctcg gcgatcgccg cgccgccgcc
     2641 tttcgcctcg acctcgaccg cttcgatctc gatgtccttt tccggactga tgaatccgaa
     2701 acgcttttcg tgcgcgatct cgaacagcgc gcgcatgccg cgcaagctgg ccacgggaat
     2761 gggaagcgcg ctgtcggttc cggcataacg gagatgcgcc tgggccgtga ttgcgatcgc
     2821 gccgccatcc acgccctggc gcaggagctc ctgccgcact tcctcttcga gccccgcgcg
     2881 aatcttggcg agcgcggcaa gtccctcctc gtcgagccgc gcgagcacgg tcttcgtgcg
     2941 cgtggcgctc agcgcggcaa gccccatgcc gaaggccgac aggacgcccg acagcggatg
     3001 gatcagaacg gactgaatgc cgagcgcgtc ggccacgagg caggcatgtt ggccgcccgc
     3061 gccgccgaag gagttcagca cgtactcggt cacgtcgtag ccgcgctgga cagaaatggt
     3121 cttgatcgcg ttcgccatgt tctcgactgc gatcttgatg aaaccgtcgg cgatctcttc
     3181 gggcgcgcgc gccagcccga gcttgcgggc aagcgacttg aacgccttga gcacggactc
     3241 cgcgtcgagg ggctgatcct gccttgggcc gaaaatgcgg gggaagaaat cgggcgtgag
     3301 cttccccgtc atcacattgg cgtcggtgac ggtaagagga ccgttgcgcc ggtagcagag
     3361 ggggccggga tccgcgcccg cggagtcggg tccgacgcga aaacgcgtct catcatagtg
     3421 gaggatcgag ccgccgcccg cggcgacggt atggatcaac atcatcggcg cgcgcacgcg
     3481 gacgcccgcc acttggctct cgaagctccg ctcgtaggcg ccgtcatagt ggcagacatc
     3541 ggtcgaggtg ccgcccatgt cgaagccgat caccttggcg aagcctgccg cctgcgcggt
     3601 ttcgaccgct cccaccacgc cgcccgccgg ccccgacagg atcgcgtcct tgcctctgaa
     3661 cagtcccgcc gatgtcagtc cgcccgacga ctgcatgaac aagagtcgtg gggctttagc
     3721 ctccccctcg tggggaggcc ggccgacgaa gccggccggg tggggggctg tctgtcgcct
     3781 caccccaccc ggctcggctt tcagcctcgc caccctcccc atcaatggga gggatgaaga
     3841 tggtttcagc gcgcgagcca cgcgatcgac gtaacgccgc agcagcggcg acaggtacgc
     3901 atcgaccacg gcggtgtcgc cccgcccgac gaacttcacg agcgggctga cctcatggct
     3961 cgccgagact tgcgtgaaac gttcctcgcg cgcgagcctc gccgcctcgc gctcatgcgc
     4021 ggggaaggcg taagaatgca tgaaggcgat agcgacggcc gagatgccgt cggcgcgcgc
     4081 cgcccgcaaa gccgcccgcg tcgcgtcgcg atcgagcggg acctcgacgc cgccatcggc
     4141 gcgcaggcgc tcgggcacct cgacgacgcg ggcatagagc atcgaaggct tttcgatccg
     4201 ccgcgcgaag atcttgggtc gcgcctggta cccgatctcg agcgcgtcgc ggaatccctg
     4261 actcacgatc agaagcagag gatcgccctt gcgctccaac agcgcgttgg tggcgaccgt
     4321 cgtccccatc ctgatgcttg cgatcgcttg cgaagggagc ggccggttgc gcgggacgcc
     4381 gatcaggtcg gcaatgccgg cgagcgcggc atccgcatag gcctcgggat tttccgacag
     4441 cagcttccgt gtcagcagcc ttccgtcagg cgcccgcgcc acaatgtcgg tgaacgtgcc
     4501 gccgcgatcg atgaagaatt gccacgtgcc gccgccgctc atccgatgcg cttgagctcc
     4561 gcggcatagc gtttccagcg cttgacatag acttccgtgg cgcgcgcgat catcgcccgc
     4621 gctgcgtcat cgctcgcgcg cacggacttg gccggtgcgc cgaggatcac cgtatcgtcg
     4681 ggaaacgcct tgcccttggt gataagcgcg ccggcgccga ccaggcaatt cgacccgatc
     4741 ttcgcgccgt tcaggacgat ggctcccatt ccgatcaggc tcgcatcgcc gatagtgcag
     4801 ccatgcagga tgacattgtg gccgatgacg cagtcttccc cgatcgtcag cggaaacccc
     4861 gggtcggtgt gaagggtcgc gttgtcctgc acttgcgagc gcgcgccgat ctcgatccac
     4921 tcattgtcgc cgcggagcac cgaaccgtac cagatgctca catccgaatg gagccgcacc
     4981 cggccgatca ccgcggcggt ttccgcgatc cagtagagcc cgtccgctgg aaactcgggc
     5041 cgtgtcccct cgagctcgta aaccgccatg ccgattccct ctaccgagcc gggctctttg
     5101 cctccggctt tgcagcgcgg agcgcccgtt cgaggcgtgc aatctcgtcg cggagaacgc
     5161 cgttctcaag ctccgcatac atcgccgcgg cttgggcgcc tagtctcgcg agcatcgccc
     5221 gctcgcttgg atcgaggtcg gtgcccgatt catgcggtcc gtaaagcgag acggcgaaac
     5281 agcgcacggg attgacggcg ggcacggcaa gaaccgggcg ggcaagaccg gtggggaggt
     5341 cgataccatc ctcatcttga tccgcaacac tgaaaggctt gccttcgctc acgggcgcga
     5401 gcaatggcac atcgggtttg atgctcgtcg ccgtgccgcg gctccagccc ttgccattgc
     5461 cgtcgcgcct gaacgccagc ccttcgcggc ggaacgcggc ggcagaagcg agcttcagcg
     5521 ccttgaaagt atcgtcggcg agcagcctgt cgatctcgcc ggccttcttg gcttggcgga
     5581 tggcatgccc gagccttcgc tccgcgtcgt cgaccgccct gttgaaatag cgatcggcaa
     5641 agtgaacggc cccttcatgc aagcgggtga gcaagaatat tgcggccgcc ccgagagcga
     5701 tccacgccca acccggcaga ttgagatatt tttgcatgcg ttccacctcg tggtgcagca
     5761 agaggaaggg aacgctgagg gtgaggccca ggatcgtcac gcggcgcaag ggaatcgtca
     5821 cgttcacaac gcgcggccgg cgcaacgcct cgaacacgaa caggcacagg acgccgttga
     5881 cgaggtagag gagcccgatg acgtcgtcgc tcggcgtgaa gttcccccac cgtgtgtgga
     5941 agatggtggt ggcgcaggca agctcgccga ggagaaatgc gggcagtccg atgagacagc
     6001 cccagatcac ccagcgaacg cgctgatagt cctcgggcgt ctgcgttcgc cgcctgacca
     6061 taagaatggc gagcgcgccg gcagcgacga ggaagcccat gagaatcgaa atgcgggtca
     6121 tcgtctcgac cggatagccg aaaaggctgc cgtaggaggc gaacagcagt agcgcaaaga
     6181 caacggcaac gatcggcaag gcgcgttcaa ggccgcgcca tcgggcttcc gtctcgttgt
     6241 tcggcacgcg cagcacgaag agcaggaggc cggcatagcc tatggcctgc gcgagaacca
     6301 tcgcgatgtc cgtcacaaag aagagcagcg gcctcggctc gaggagggcg tagaagccat
     6361 ggacctgccc cggattgaac cagttcacgt aaaggaagaa accccagctc atcgccgatg
     6421 gtcgcgtcca aaccagccac gcggcggcaa gcacgacgag gatgccggca atctgacaaa
     6481 gaaaattcac gccgcggacg agggcatttg acggcggcgg ctctgccacc agggacacgt
     6541 gccgcgcggg ctgtccatcg gccgcgacaa gatcgagcgt gacggtccgc cccggcaaca
     6601 gatattgcac gccgccaagc gtggcgatcg cgctgctgca cttttcgagc gcgccgagcc
     6661 tgcagcgcat ggcttcaaga tcgagccggt cgccgaccct gatcccggcg cgccacgcgg
     6721 gcgaagcggc ttcatcgggg aaaggttcgg tcacgtcggt gatcagtccg tcgccgtcgg
     6781 caaaaagccc tatggaggcg aggggctccg caactctcag aaggtcgggg aagatcatca
     6841 cgaggcccga cagagtgagc gcaatgagca gagatcgacc agcggtcatt ggcgacatcc
     6901 tcaagccccg gcggatgtgg agaatcgcaa cgatacccat tgccaggccc gcgtaaccat
     6961 tccgcaattg cggaatctgg caaggggaaa ggtgaggtga gagaggcccg ccgtcagggg
     7021 ccctgcaagc ccttgtaacg cctaaccccc tcggctatga gggtcaccgg cttggctcaa
     7081 atcccctaat cgatccgcat ggataccgaa gctcccaagg gaacgccgcg gcacggcggc
     7141 aacattctca ccatcttcat cacctcgctc tgcgcggcca tcgttgtcgc gggcgggttt
     7201 ggcatcgtgg tgcaagccgc catcctcgcc gcggtggccc ggcttgggct cggcgagacg
     7261 gtgctttgga tcggaagcgc gatcaacgcc gcgctgacgc tttggctcgc gctctggacc
     7321 ttcgcccgat cgtggcacgt tgaacggcgg ttgaaggccg gccttgaagt ggacgagccg
     7381 aagctctcga tcctggccaa tttccgcggt tcttgaccgg gcggaaccat gaggcccggg
     7441 acgtgattct tcacatgcgg cccaggtcaa aagtccttat gctctcgcca aagaagcaaa
     7501 gtcgggggga aatccgctaa tgtcgatttg gggcaagctc gcaggcgcag cggcaggact
     7561 cgcggtcgga ggcccgatcg gggcgctgct cggcggtgtg gccggtcact atgtcatcga
     7621 tcgcgacaag gaagaagaag ggccggccga gaaccaggtg gccttcaccg tgggcgtcat
     7681 cgcgctcggc gcaaagatgg ccaaggccga cggcgtcgtc accatggacg aggtcaacgc
     7741 cttcaaggag gtcttcaagg ttcccgatgg cgagatgaag aacgtggcgc gcgtcttcaa
     7801 tctggccaag caggacatgg ccggctacga ggcttatgcc gagcagctct ccaccatgtt
     7861 caagggcaac cgcaaggttc tggaggacgt gctcgaaggc ttgttccata tcgccaaagc
     7921 cgacgaggcg ctacatccgc gcgaggagca attcctgggc ggtgtcgcca agcgcttcgg
     7981 catgaccgac accgagttcg cctatatcaa ggcgcgccac gtcgccgccg ccaagcgcaa
     8041 tccctacgac gtgctgggca tcaagccgtc gatcagcaac gaagagctca aaagtcacta
     8101 tcggcggctg gttgccgaga gccatcccga caagctgatt gcgcgcgggg tcccgaagga
     8161 gttcgtcgtg ctcgcaaccg agaaggtcgc gaccatcaac gaggcttacg accagatcgc
     8221 caaagagcgc gggatgtagt tttgccttcg ccggtgtcat tcccgcgcag gcgggaatcc
     8281 agtaaccgcc gacctggatt tgtggcgctc gccggggtta ccggatcgcc cggtcaagcc
     8341 gggcaatgac aatgctgatg acatgaccgt cgcccccgac agtccgctcg ccacgctgtg
     8401 gtgtccgtcg ccgaaccgcg aagcgcggcg gaacgggatt aagcccgaca tgctgctgtt
     8461 gcattacacg ggcatggagc aggcggagag cgcgctcgac tggctgacgc gccaagagtc
     8521 gggcgtgtcg tgccattact ttatcgacga agagggacgg atcgcgcagc tcgttcttga
     8581 gtccgagcgc gcctggcact ccgggcaaag cctttgggcc ggcgagaccg atctcaattc
     8641 ctgctcgatc ggcatcgaga tccacaatgc cggtcacgat ttcgactatc cccatttcac
     8701 cgaggccgag atgcgggcgg tcgaggcctt atgccgcgat atcttgagcc gtcacgcgat
     8761 tccggcgcat cgagtgctcg ctcattccga cgtggcgccg gggcggaagc gcgatcccgg
     8821 cgagaaattc gattgggagc ggctcgcgcg cgccggcatc ggcctctggg tgccgcccac
     8881 gccgctcggg gacgataccg gtcttgggcc cggcgatgag ggcgagagcg ttgccgcgtt
     8941 gcagcgcgca ttgcttgact tcgggtatgg cgtcgaagtg acgtccacct acggcacggg
     9001 gctcgagaag gcggtcgaag ccttccagcg ccacttccgg cccgcgcgca tcgacggccg
     9061 tgccgatgcc tcgacgagcg acacgctcga tcgcctgctc gttgcgcggt ctcgggccgc
     9121 gatagtctga ggctcgacaa gaaag
//
LOCUS       DASUQP010000157        15348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_214942,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000157 DASUQP010000000
VERSION     DASUQP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15348
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214942"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1203
                     /locus_tag="VFY74_06930"
     CDS             142..1203
                     /locus_tag="VFY74_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5931468.1"
                     /translation="MRVRLKGVASATKRLASGEKVKYYYAWRGGPQLLGKPGSPEFVQ
                     AYEQAHRERKQSNPALFRSIIAGYLASPEFADVRDRTKSDYQKQLVKIERAFGDLPIV
                     ALDDPCVTKEFLEWRDGFASSPRQADYAWTVLMLLLAWARSRGLTAYRPPGRIKRLYH
                     GDRSDKIWEEHHITAFLSVAPPCLQRAPLLASDTGQRQGDLLKLPWSAYDPTPTPESP
                     FGWIRLTPSKSITREKPKGRTISVPVSKRLHAVISTMPRVSPVMLTNSYGRPWQGNSF
                     RKAWGAVAAKASISELTFHDLRGTGVTRLSEADCTPQQIARFTGHSLRDVAAILDRYL
                     ARTDKLASMALAKLERARG"
     gene            complement(1256..1329)
                     /locus_tag="VFY74_06935"
     tRNA            complement(1256..1329)
                     /locus_tag="VFY74_06935"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1295..1297),aa:Cys,seq:gca)
     gene            1553..2233
                     /locus_tag="VFY74_06940"
     CDS             1553..2233
                     /locus_tag="VFY74_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5931469.1"
                     /translation="MSDDARLRLTMVETQLRPNAVTDTRLLAAMGALPRERFVPNRFR
                     ALAYIDEGIEVFPAIDAAPARYLLAPMVLARLIQLAAVEPGDTVLDVGCATGYSTAVL
                     ARLGRQVIGLEPEPELAQAARANLRDLGLAGADIVEGSLAKGCPEKGPYDVIVLNGGV
                     PGPPEALLAQLKEGGRLAAILSRGGPGTQGRAYSFVKAGGEASGVPHFDAGARPLPGF
                     SPEPSFSF"
     gene            2415..3986
                     /locus_tag="VFY74_06945"
     CDS             2415..3986
                     /locus_tag="VFY74_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX5931470.1"
                     /translation="MSGHDMSSCLTLSLRPYGGAFVVCAFLVISAFGRPAAAETLEEA
                     LADAYLINPVLNAERARLRATDEEVALAKSGLRPFIFGTADTAFLNTNTTTSGGANNT
                     APSNIGLLTGPVKQAFPVNTGNAGENLTATGAIPLNQAFGQGEADTDDGVTNPRGYAV
                     QLNQPIFEGFRNLNAIREAKATVQAGREALRTVEQTVLLDAVTAYVNVVRDGAIVRLR
                     ENDVSVLSEQLKATRDRFDVGEVTRTDVAQAEARRSEALATLAAAQANLKTSRAAYEQ
                     VIGHPPGNLVTPASIRHLLPSTLNDAMTLGDGENPLILAAVYNEEASLYAVQRIMGEM
                     LPDVQLEARYEKRFDLGSTVESQEQTQVIGRLNVPFYQGGGVSARVRQAKETNNQLKK
                     EVEDARLRVHADVIANYGILQSSGPAISSAEAAVAANKIALTGVREEEKVGQRTTLDV
                     LDAQRELLNSQIGLVTALRDRVVAEYSLYTAIGRMDAQTLGLPVPYYDPIEHYDIVKK
                     KLFGLRPPPPPAPDE"
     gene            4146..5153
                     /locus_tag="VFY74_06950"
     CDS             4146..5153
                     /locus_tag="VFY74_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX5931471.1"
                     /translation="MSSTERAPEPTMEEILASIRRIISDDESNAARPRPDSATQQADE
                     GPEVAEGDADDKIIDDIARVLSASAPKVEDEEDILDLTAELSGPEAVEEVLVEEMLVE
                     EVAAPLEMPPDEIFPPEPAGAGFLAAEAPPPEPEVSAPPAPPLSASEEAASALERAIA
                     ALRAGQVPTSVSEFMPYREPVPEPAPEPRAFTPVSEPVAEPEPVPEPLPELEAEPEPQ
                     LVLHDFTAETVAEETVPVETPFESTPEESVTPPSESVPWATAASAPYVEPERPATRRN
                     GGTTHGASHLETSHKSLEDSIKDMLRPMLKQWLDENMARVLTAALKDELGDRQSRGQS
                     E"
     gene            5230..7956
                     /locus_tag="VFY74_06955"
     CDS             5230..7956
                     /locus_tag="VFY74_06955"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5931472.1"
                     /translation="MLDKTYQPQRIEGAIYRAWEEGRAFAAGRPDRAEAKPFAIVIPP
                     PNVTGSLHMGHALNNTLQDVIVRFERMRGRDVLWQPGTDHAGIATQMVVERQLMERQE
                     QGRRAMGRDAFIRRVWQWKEESGNTIVGQLKRLGCSCDWTRLRFTMDEGLSRAVRKVF
                     VDLYRAGLIYKDKRLVNWDPELLTAISDLEVEQIETKGHLWHLRYPIEGKPFNAGDPA
                     TFIVVATTRPETMLGDTAVAVHPSDKRYKHLVGKNAILPLVGRRIPILADEYSDPEKG
                     SGAVKITPAHDFNDFEVGRRHGLPMVNVLSPEAKLLLDPNPDFTQGVEANPELRATIL
                     ELNGVFRFAARKLIVERLATQGLIEKIEPHVHTVPHGDRSNAVIEPRLTDQWYVDAAT
                     LAKPAIDAVKRGRTVFVPKNWEATYFHWMENIQPWCISRQLWWGHQIPAWYGPDDKVF
                     VAEDEAAAQAQAKENYGKAVALTRDEDVLDTWFSSALWPFSTLGWPEKTPELARYYPT
                     DVLITGFDIIFFWVARMMMMGLHAMEEVPFRDVYIHALVRDEKGQKMSKSKGNVIDPL
                     DLIETYGADALRFTLAAMAAQGRDIKLSAARVEGYRNFATKLWNAARFAEINDCVPRA
                     DFDPAAAKVTLNRWIAGESERTLAAVTEALEAFRFNEAAGAIYHFIWHVYCDWYLELC
                     KPIIAGGDDAAAETRAMAAFVLDRALTLLHPFMPFLTEELWAKRAPEEGRGSLLILAP
                     WPAHQGLADAEADAEIGWLIRLISEVRSVRAEMNVPAGAKVPLIIGGASEETKARAAH
                     YEETLLRLARLERVEFGKPPTGAVQIVLDEAVLALPLADIIDIDAESKRLKREIDKVG
                     SEIRQLDAKLANEKFVARAPEHVVEEQRERKTEAEAMAAKLEQALKRLEAAL"
     gene            complement(7975..8745)
                     /locus_tag="VFY74_06960"
     CDS             complement(7975..8745)
                     /locus_tag="VFY74_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931473.1"
                     /translation="MAQAKLHLPDPKWAQGPYRPFTSDPGFNEAWWWLGVPVLVAAFT
                     IGTYQIAPEWYRRYVLPEGYGLLEVTHFVLPLLGLLIACGLLFLPFVRARPLVFTVAL
                     IGAFSCLYIAGEEISWGQHFFHWSTPEYWAEVNRQQETNLHNTYVIFEKTPRSILEVG
                     IFFGGLLVPLAALFVPWLRACRMSLFLPPAALVPAALGAVIFKLIDRLQQGASIPTVL
                     ERPSETIETYLYFFIFAYLLIFARRIKELELAEGAVPK"
     gene            complement(8751..9584)
                     /locus_tag="VFY74_06965"
     CDS             complement(8751..9584)
                     /locus_tag="VFY74_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931474.1"
                     /translation="MAKLKATPRLGGRSRSRKAKLPLPDPKSPGPFKPFMGDPGWNEA
                     WWWLGIPLVAALFVVGSYAIDAEWSRRWVTGESGILETAQFIFMVIGFAIAAQLLFAP
                     FVRRRPLVLAVTAVAALACLFIGGEEVSWGQHIFFWQDPSLVTAVNDEGELSLHNMNK
                     GFERGPRTLLEIGVVTGGLLVPLLCAYAPWLRQSRMALFLPAAALVPAALLAVTFKGA
                     EMLAKYHGAGLGVAARPSEAVELYFYFFMMAYLIVFERRIGELETEGARTKARAKAKK
                     T"
     gene            9921..11645
                     /gene="ilvD"
                     /locus_tag="VFY74_06970"
     CDS             9921..11645
                     /gene="ilvD"
                     /locus_tag="VFY74_06970"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5931475.1"
                     /translation="MDAKTFDKSRLPSRHVTEGPSRAPHRSYYYAMGLTRDQIHQPFV
                     GVATCWNEAAPCNIALMRQAQAVKAGVIAANGTPREFCTITVTDGIAMGHQGMKSSLP
                     SREVIADSVELTMRGHCYDALVGVAGCDKSLPGMMMAMCRLNVPSIFLYGGTILPGSF
                     RGRAVTVQDLFEAVGQYSVGKMSTEDLDELEQVACPSAGACGAQFTANTMACVSEAMG
                     LALPYSAGAPAPYEIRDRFCQAAGEKIMDLIALNIRPRDIVTREALENAAAVVAATGG
                     STNAALHLPAIAHECGIDFDLFAVADVMKRTPYVADLKPSGRYVAKDLFEAGGVPLLL
                     KTLLDHGYLHGEGMTVTGRSLKDNLAKVKWNPDQDVVRPADDPISPTGGVVGLQGSLA
                     PDGAIVKVAGMKRLKFRGPARCFDCEEDAFAAVSAKRYNEGDIIVIRYEGPKGGPGMR
                     EMLSTTAALYGQGMGDKVALITDGRFSGATRGFCVGHVGPEAALGGPIALIRDGDIIA
                     LDAEKGTLDVELDASELKKRAKAWTAPPMRFKSGALWKYANEVGPARTGAVTHPGGGV
                     EVACYADV"
     gene            11638..12621
                     /locus_tag="VFY74_06975"
     CDS             11638..12621
                     /locus_tag="VFY74_06975"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5931476.1"
                     /translation="MCNRLALGALAALLTMGPMMGPVAVGADDPPPYPSATEAYRQGV
                     KALKAGRPVTAVPALEYAAKRGVLGAQVKLARGYASGRDVPKDDAKAFAYFEQIADQQ
                     ADISPSSPVAKYAAEAFVALGQYYLDGIPGKPLEANPSYAADLFRHAASYFGDAEAQY
                     RLARLYMNGTGVEKNVGLAVNWLATAAKKQHAASQATLGEILWRGQDVRERRARGLAL
                     IILAHENARAGGKEPQWIGALYDEAFAKSDKATRKDAQALLPALSSVKATSVLPSEAK
                     PSEIFVMPAQEKAQAPGMPASDRSGPGAPQPAPIGMSVGFGGNRGEAGFKP"
     gene            complement(12650..13432)
                     /gene="xth"
                     /locus_tag="VFY74_06980"
     CDS             complement(12650..13432)
                     /gene="xth"
                     /locus_tag="VFY74_06980"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5931477.1"
                     /translation="MKIASWNINGIKARIEAATTWLKAASPDIACLQEIKCVDEAFPK
                     EQFEELGYNVAVHGQKGFNGVALLSKLPFDEVHPRLAGDPDDVQARYLEAVISVPSGA
                     LRLINIYLPNGNPFGTEKFVYKLAWMTRLKERVRALLANEEKLIIAGDFNVIPAPEDV
                     YNPSVWMDDALFRPETRAHFHALLNLGLTDAFRACHDEPHRYTFWDYQAGAFQKNQGL
                     RIDHVLLSPQAADRLQACEIDAGPRSWEKPSDHVPIVAELAL"
     gene            complement(13429..13701)
                     /locus_tag="VFY74_06985"
     CDS             complement(13429..13701)
                     /locus_tag="VFY74_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931478.1"
                     /translation="MSLQTQKQTLPLMGRAARPKAELGEVMVFPLAIALLLEAQTPHP
                     DAAFGAVDLPTRGTFEKARNMRAHLMLWQAWATGIARQFEHSAIHP"
     gene            complement(13794..14126)
                     /gene="erpA"
                     /locus_tag="VFY74_06990"
     CDS             complement(13794..14126)
                     /gene="erpA"
                     /locus_tag="VFY74_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX5931479.1"
                     /translation="MSEASITISDRAANRIAEILKGEAALSLLRVSVEGGGCSGFQYR
                     FDLVTERAPDDLLIERDGARVIVDPVSLGFLHGAELDFVDDLIGAQFKLNNPNVTAAC
                     GCGTSFSI"
     gene            14174..>15348
                     /locus_tag="VFY74_06995"
     CDS             14174..>15348
                     /locus_tag="VFY74_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX5931480.1"
                     /translation="MSIDHGRKAEGTFAARPASGDLTAEAAPARVPYAVDPSASRGRL
                     QPEPPRDTRSPYARDRDRILHATAFRRLNYKTQVFVYHEGDHYRSRLTHSLEVAQIAR
                     ALARTLRLDEDLTEALALAHDLGHPPFGHAGERALDAAMEGSGGFDHNAQSLRVVAAL
                     EKKYARFDGLNLTWETLEGLVKHNGPLTGPHAPRQRAVPEPIRDYCAKQDLELETFAS
                     VEAQAASLADDIAYNNHDIDDGYRAGFFTFAELAEVPIAARALAEVREAYPGIDGTRL
                     LYEAIRRLIGAMIEDAVAESERRLGALQPLSAADIRHAPHAVVAFSEEMRRDLDGLRA
                     FLFARVYRHERIVRIMADAEGVVRDLFRRYSAEPETLPPDWRGPAPPCGTRAYARHVA
                     "
BASE COUNT         2912 a         4940 c         4857 g         2639 t
ORIGIN      
        1 tcttcaagca actcgccagc gcaggcttaa ttccgtcaac gattttgata gagccgaacg
       61 gcgcaacgaa aattagcttc acatcgtcgg aggaaaattc ggcgctggag acgcctcttg
      121 aggcatggag gaagaagcgc gatgcgggtt aggctgaaag gtgtagccag cgcaactaag
      181 cggctcgcta gtggggagaa ggttaaatac tattacgcat ggcgcggagg cccacaactt
      241 ctgggcaagc cgggatcacc cgaatttgtt caggcttacg agcaagcgca ccgcgagcgt
      301 aaacaaagca acccggcact cttcagatcg atcatcgcgg gttatcttgc ctcacccgaa
      361 ttcgccgacg ttcgcgaccg gaccaagtcc gattatcaga agcagcttgt taagatcgaa
      421 cgcgcgttcg gcgatcttcc catcgtggca ttagacgacc cttgcgtgac gaaggaattt
      481 cttgaatggc gcgatggttt tgcatcgagc ccgcgtcaag ctgactatgc ttggacggtg
      541 cttatgctgt tgctcgcatg ggcgcgaagc agggggctca ctgcataccg tccgcctgga
      601 cgcatcaagc gtctgtatca cggcgaccgc tcagacaaaa tttgggagga gcaccacatc
      661 accgctttct tgtcggtagc gccgccatgt ttgcaacgag cgcctttatt agcatccgac
      721 acaggacagc ggcagggcga tcttttgaaa ttgccatgga gtgcttacga tcctacgccc
      781 actcctgaat ccccattcgg ctggatcaga ctcacgccat cgaagagtat cacgcgagag
      841 aagcctaagg gacgaacgat ttccgtgccg gtaagtaagc ggctacacgc ggtgatcagc
      901 accatgccgc gcgtgtcgcc ggtgatgctg actaatagct atgggcgacc gtggcagggc
      961 aactcattca ggaaggcttg gggtgcggtc gccgctaagg cgagcatctc cgagctaacc
     1021 ttccatgact tgagaggtac cggagtcacc cgcctatcgg aagccgactg caccccgcaa
     1081 caaatcgcac gcttcaccgg ccacagtctc agagacgttg cggcgatcct cgatcgttat
     1141 ctcgcgcgca ccgacaagct cgcgtcaatg gcgctagcta aactagagag ggctagaggg
     1201 tgaacagaat ttgcaaaccg actgcaaacc ggaacacttc cgtgttgcta agtattggag
     1261 gccacgaccg gaatcgaacc ggtgtgcacg gatttgcagt ccgctgcgta accactccgc
     1321 cacgtggccg atttgtggag agaagccccc taactaagtg atcctccccc tctcatgtca
     1381 atacgcgatc aacgcagagg gaggaaagcc ccgcgttctc aaacctccca cgagaggagg
     1441 tttgaaaatt cccctctccc gcttcaagtg gcgaagacta tcggcaattg ggcaccgatg
     1501 ctaagagatc cccgggggct cgatgaaatc gcaagaagcg gggcaagaag gcatgagcga
     1561 cgatgcgcgt cttcggctca ccatggtcga gacgcagctc aggccgaacg cggtgaccga
     1621 cacgcgcctc ctcgccgcca tgggcgcgct cccgcgcgag cgcttcgttc cgaaccggtt
     1681 tcgcgctctg gcctatatcg acgaaggaat cgaagtcttt cccgcgatcg acgccgcgcc
     1741 ggcgcgctac ctgctcgctc ccatggtctt ggcccggctc atccagcttg ccgctgtcga
     1801 acccggcgac acggtcctcg atgtcggctg tgccaccggc tattccacgg ccgtgctcgc
     1861 gcggcttggc cggcaggtta tcgggctcga gcccgagccg gaacttgcgc aagcggcgcg
     1921 cgccaatttg cgcgatctcg ggcttgccgg tgcggacatc gtcgagggat ccctggccaa
     1981 gggatgcccc gagaaaggtc cctacgatgt gatcgtcctc aacggcggcg tccccggtcc
     2041 gcccgaggcg ttgcttgcgc aattgaagga gggcggcagg cttgccgcga ttctctcccg
     2101 tgggggtcct ggaacccaag gaagggccta ttccttcgtc aaagccggcg gcgaggcaag
     2161 cggcgtgcca cattttgacg ctggcgccag accgctccct ggcttttccc ccgagccaag
     2221 cttcagtttt tgagcttcgg ccccctcggg ggcactgttg ccccgatccc acagtctctt
     2281 gccctttttt cgagaatccc aagcaaacca acttttcgtg ggtatcgcac cctgactgtg
     2341 gtgctctaag gtttgaccgc agtagggagt cgggggactt cacgaactgc gggagatacg
     2401 gtgtgcgagg cgtcgtgagc gggcacgaca tgtcgtcctg cctgacattg tcacttcggc
     2461 cgtatggcgg ggcttttgtt gtctgcgcgt tcctcgtcat cagtgccttc ggccgtccgg
     2521 ctgccgccga gacgctcgag gaagctttgg ctgacgccta cctcatcaat cctgtgctca
     2581 acgccgaacg ggcgcggctg cgcgcgaccg acgaggaggt tgcgctcgct aaatctggcc
     2641 tgcgcccctt catctttggt accgccgata cggccttcct gaataccaac accacgacca
     2701 gcggcggagc caacaacact gctccttcca acattggttt gttgacgggc ccggtcaagc
     2761 aggcattccc agtcaatacc ggcaacgcag gcgagaacct caccgccacg ggagcgattc
     2821 cccttaacca ggcattcggt caaggagagg ccgacacgga tgacggggtg accaatccgc
     2881 gtggttatgc ggttcagctc aatcagccga tcttcgaggg cttcaggaat ctcaacgcca
     2941 ttagagaagc gaaggctacc gtgcaggcgg gccgcgaagc gttgcggacc gtcgagcaga
     3001 cggtcctgct cgatgccgtg accgcttacg tcaacgttgt gcgcgatggg gccatcgtgc
     3061 gcctgcgcga gaacgacgtc tccgtgctct ccgagcagct caaggcgacc cgggatcgct
     3121 tcgatgtcgg cgaggtgacg cgcaccgacg tggctcaagc cgaagcgcgg cgctccgagg
     3181 cgctcgccac gctcgccgcc gctcaggcca atctcaagac gagccgcgca gcctatgagc
     3241 aggtcatcgg tcatcccccc ggcaatctcg tcacccccgc ctcgatccgg cacttgctgc
     3301 cttcgacgct caacgatgcg atgacgcttg gcgacggcga gaaccctctc atcctggccg
     3361 ccgtctataa cgaggaagcg tccctttacg ccgtccaacg gatcatgggc gagatgcttc
     3421 ccgatgtgca gctcgaggcc cgatacgaga agcgtttcga tctcggctcg accgttgagt
     3481 ctcaggagca gacccaggtg atagggcgcc tcaacgtgcc cttctatcag ggcggcggcg
     3541 tctcggctcg cgtgcgccaa gccaaggaga ccaacaatca actcaagaaa gaagtcgaag
     3601 acgcgcgatt gagggtccat gccgacgtca tcgcgaacta cggcatcttg cagtcctccg
     3661 gacccgccat cagctcagcc gaggcggccg tcgccgccaa caagatcgca ctcaccggcg
     3721 tgcgcgagga ggagaaggtg gggcagcgca cgacgctcga tgtgctcgat gcgcagagag
     3781 agctcttgaa ttcgcaaatt ggccttgtga ccgcgctcag ggaccgcgtc gtcgccgaat
     3841 attccctgta caccgcgatc ggccggatgg acgcccaaac gctcggtttg ccagtgcctt
     3901 attacgatcc gatcgagcac tatgatatcg tgaagaaaaa gctcttcggt ttgcgcccgc
     3961 cgcccccgcc cgcccccgac gaatgaacca aaaagcctgg ctggagttaa cttaggtcga
     4021 tattcgaccc acgaaatcgg gacttgggga ttaacgctgt gttaagctag atagccaagg
     4081 ggcgacccgg gtgttttgac cgccgggcga tgcgggagtt tgatatgggc gcaagggatc
     4141 aagtgatgag cagtacggag cgggctcctg agccgacaat ggaggagatt ctcgcctcca
     4201 tccgtcggat aatctccgac gacgaatcga atgcggcgcg gccgcgtcct gactctgcga
     4261 cgcagcaggc ggacgagggc cccgaggttg cggaagggga cgccgacgat aagatcatcg
     4321 acgacatcgc gcgcgtcttg agcgcgagcg cgccgaaagt cgaagacgag gaagacattc
     4381 ttgacctgac cgccgagctg agcgggccgg aggccgtcga ggaggttctg gtcgaggaga
     4441 tgctggtcga ggaggtggcg gcaccgctcg agatgccgcc tgacgaaatt tttccgccgg
     4501 agccggcagg cgccgggttc ctggcggccg aagctccgcc accggagcct gaggtctcag
     4561 ccccgcccgc gccgccgctg tcggcgagcg aggaagcggc atcggcgctc gaacgtgcga
     4621 tcgccgcgct gagggcgggc caagtgccga cctccgtctc tgaattcatg ccttaccgtg
     4681 agcccgtgcc agagcccgcg cccgagcctc gagcgttcac gcccgtgtcc gaacctgtcg
     4741 ccgagcctga gcctgtgccc gagcctttgc cggagctcga ggctgagccg gagccgcagc
     4801 tggtcctgca cgacttcacg gcagagaccg tcgctgagga gacggtcccg gtggagacgc
     4861 cgttcgagag cacgcccgaa gagagcgtga ccccgccgtc ggaatcggtt ccttgggcca
     4921 cggcggcctc cgcgccgtat gtcgagcccg agcgccccgc cacccgaagg aatggcggga
     4981 cgacgcacgg agcatcacat ctcgagacct ctcacaagtc gctcgaggac agcatcaagg
     5041 acatgctccg cccgatgctc aagcaatggc tcgacgagaa catggcgcgc gttctgacgg
     5101 cagcgctcaa ggacgagctt ggggaccgcc aatcgcgcgg ccaaagcgaa taggcgcatc
     5161 tgagttgaca gtgcggaagg caagcgcgta ggacgcgggg ccaagcgacc gttacgccac
     5221 cccccttcca tgctcgacaa gacctatcag ccgcaaagga tcgaaggcgc gatctatcgc
     5281 gcctgggaag agggtcgcgc attcgccgcg ggccggcccg atcgtgccga agccaagccc
     5341 ttcgccatcg tcataccacc gccgaacgtg accggctcgc tccatatggg gcatgccctc
     5401 aacaacacct tgcaggacgt gatcgtgcgg ttcgaacgca tgcgcgggcg cgacgtgttg
     5461 tggcagccgg gcaccgatca cgccggcatc gccacccaga tggtggtcga gcgccagttg
     5521 atggagcggc aggagcaggg ccgccgcgcc atgggtcgcg atgcgttcat ccgccgcgtg
     5581 tggcaatgga aagaggagtc gggcaacacc attgtcgggc aactcaagcg gctcggctgc
     5641 tcctgcgact ggacgcggct ccgtttcacc atggacgagg ggctttcgcg cgcggtgcgg
     5701 aaagtcttcg tcgatctcta tcgtgccggc ctcatctaca aggacaagcg cctggtcaac
     5761 tgggatcccg agctgctcac cgcgatttca gacctcgagg tcgagcagat tgagaccaag
     5821 ggccatctct ggcatctgcg ctacccgatc gaggggaaac ccttcaacgc cggcgacccc
     5881 gccaccttca tcgtcgtcgc caccacgcgg ccggaaacga tgcttggcga caccgccgtc
     5941 gccgttcatc ccagcgacaa acgctacaag catttggtcg gcaagaacgc gatcctgccg
     6001 ctcgtcggtc gccgcatccc gatccttgcc gacgaatatt ccgatccgga gaagggctcg
     6061 ggcgccgtca agatcacgcc cgcgcacgac ttcaacgatt tcgaggtggg ccggcgccat
     6121 ggcctcccca tggtcaatgt gctgagcccc gaggccaagc tcctgctcga tcccaatccc
     6181 gacttcacgc aaggcgtcga agcgaacccc gaactcaggg cgaccattct cgaattgaac
     6241 ggcgtcttcc gtttcgccgc gcgcaaattg attgtcgagc gcctcgcaac ccaaggcctg
     6301 atcgagaaaa tcgagccgca cgttcacacc gtgcctcacg gcgaccgctc caacgccgtg
     6361 atcgagccgc gcctcaccga ccagtggtat gtcgatgccg ccacgctggc gaagcccgcc
     6421 atcgacgcgg tcaagcgcgg ccgcacggtg ttcgtgccga agaattggga ggccacgtat
     6481 tttcattgga tggagaacat ccagccctgg tgcatatcgc gccagctctg gtggggccac
     6541 cagatcccgg cctggtacgg acctgacgac aaggtgttcg tcgccgagga cgaggccgcg
     6601 gcgcaagccc aagccaaaga gaattacggc aaggccgtcg cgctcacgcg cgacgaggac
     6661 gtgctcgaca cctggttctc atcggcgctg tggccgttct cgactctcgg ctggcccgaa
     6721 aagacgcctg aacttgcgcg ctactacccg accgacgtgc tgatcacagg tttcgacatc
     6781 atcttcttct gggtcgcgcg catgatgatg atgggcctgc atgcgatgga ggaggtgccg
     6841 ttccgcgacg tctacatcca cgcgctcgtc cgcgacgaga agggccagaa gatgtcgaag
     6901 tcgaagggga acgtgatcga tcccctcgac ctgatcgaga cctatggggc cgatgcgctc
     6961 cgcttcaccc ttgccgccat ggcggcgcaa gggcgcgaca tcaagctcag cgccgcccgc
     7021 gtcgaaggct accgcaactt cgccaccaag ctgtggaacg ccgcgcgctt cgccgagatc
     7081 aacgattgcg tgccccgcgc cgatttcgat ccggccgcgg ccaaggtgac gctcaatcgt
     7141 tggatcgcgg gcgagagcga gcgcacgctc gcggccgtca ccgaagcgct tgaggccttc
     7201 cgcttcaacg aggcggcggg cgcgatctat catttcatct ggcatgtcta ttgcgactgg
     7261 tatctggagc tctgcaagcc gatcatcgcc ggcggcgatg acgccgcggc cgagacgcgc
     7321 gccatggcgg cgttcgtgct cgaccgggcg cttaccctgc ttcatccgtt catgcccttc
     7381 cttaccgagg agctgtgggc gaagcgcgcg ccggaagagg gacggggctc gcttctgatt
     7441 ctggcgccct ggcccgcgca tcagggcctc gccgatgccg aggcggatgc ggagatcggc
     7501 tggctgatca ggctgatttc cgaagtgcgc tcggtgcgcg cggagatgaa cgttcctgcc
     7561 ggcgccaagg tgccgctcat cattggcggc gcgagcgagg agaccaaggc ccgtgccgcg
     7621 cattacgagg agacgctgct tcgcctcgcc cggctcgaga gggtcgagtt cggcaagccg
     7681 ccgacgggcg cggtccagat cgtgctcgac gaagccgtgc tcgccttgcc gctcgcggac
     7741 atcatcgaca tcgacgccga gtcgaagcgg ctgaagcgcg agatcgacaa ggtcggctcc
     7801 gagatcagac agctcgacgc caagctggcg aacgagaaat tcgtcgctcg tgcgcccgag
     7861 cacgtggtcg aggagcagcg cgagcggaag accgaagccg aggccatggc cgccaagctc
     7921 gagcaggcgc tgaagcgcct cgaagccgcg ctctaattcg cgcaacgggc gggactattt
     7981 cggcaccgcg ccttccgcca gttcgagctc cttgatgcgg cgagcgaaga tcaagagata
     8041 ggcgaagatg aagaaataga gataggtctc gatcgtctcg ctcggccgct ccaacacggt
     8101 cgggatgctg gcgccctgct ggagccggtc gatcagcttg aagatcacgg cgccaagcgc
     8161 cgccggcacg agcgccgccg gcggcaagaa cagggacatg cggcaggcgc gcagccaggg
     8221 cacgaacagc gccgcgagcg ggaccagcaa gccgccgaag aagatgccga cttcgaggat
     8281 cgagcggggc gttttttcga agatgacgta ggtgttgtgc agattggtct cttgctggcg
     8341 gttgacctcg gcccagtact ccggcgtgct ccaatggaag aagtgctgcc cccagctgat
     8401 ctcctcgcct gcaatataga ggcagctgaa ggcaccgatg agcgccaccg tgaagacaag
     8461 cggcctcgct ctgacgaagg gcagaaagag caagccgcac gcaatcagga gaccgagcag
     8521 cggaaggacg aagtgagtga cctcaagcag cccgtatccc tcgggaagca cgtagcggcg
     8581 gtaccactcc ggcgcgatct gataggtccc gatcgtgaac gccgcgacca aaaccggcac
     8641 gccgagccac caccacgcct cgttaaagcc cgggtccgac gtgaacggcc gataaggacc
     8701 ctgcgcccac ttggggtcgg ggagatgtag cttagcctgc gccatcgcgt tcaggtcttc
     8761 ttggctttcg ctctggcttt cgttcttgcg ccttcggtct cgagctcgcc gatacgccgt
     8821 tcgaacacga tcagataagc catcatgaag aagtagaaat agagctcgac ggcctcgctc
     8881 ggccgcgcgg ccacgcccag gccggctccg tggtacttcg caagcatctc cgcgcccttg
     8941 aacgtaacgg ccaagagcgc cgccggcacc agcgccgcgg ccggcaggaa gagtgccatc
     9001 cggctttgcc tgagccaagg ggcgtaagcg cagagcagcg gaacaagaag tcctccggtg
     9061 acgacgccaa tctcgagcaa agtgcgcggc ccccgttcga accccttgtt catgttgtgc
     9121 agactgagct ctccttcgtc gttgaccgcg gtgaccaggc tcgggtcctg ccagaagaaa
     9181 atgtgttggc cccagctcac ttcctcgccc ccgatgaaaa ggcaggccag cgccgccaca
     9241 gcggtcacgg cgagcaccaa gggacggcgc ctcacgaagg gagcgaacaa gagctgcgcc
     9301 gcaatggcga agccgatgac catgaagatg aactgcgcgg tttcaaggat gccggattct
     9361 ccggtcaccc accgccgact ccattccgca tcgatcgcgt aactccccac cacgaagagc
     9421 gctgcgacaa gcgggatgcc aagccaccac caggcctcgt tccagccggg gtcgcccatg
     9481 aaaggcttga aggggccggg cgacttcgga tcggggaggg ggagctttgc cttgcgcgag
     9541 cggcttcggc cgcccaagcg cggcgtggcc ttcaacttgg ccattggacc cccgttgagc
     9601 gtggcaaaag cgtccatgac gctgctgcga caatcgcgag gtttgggcct ttccgcccgg
     9661 aaacgcagac tgaagattga tgttaaggaa gctcgcctaa aatcctgaag ctgtagctaa
     9721 attgcaacaa cctgcggcat tttcgcctcg tttgcgccat aaaactttga cacatcgaga
     9781 cttgcaggag gagcgtcggc cacaagcggt tcccatggct ctgatttggg ggacgggcca
     9841 ggagaaacga gggtcgagcc attggggaag cgtggcccga gccacatatt tcccctcgca
     9901 cctcacccgg gcgttagcag atggacgcga agacgttcga taagtcgcgc ttgccgagcc
     9961 ggcatgtgac cgaggggcca tcccgcgccc cccaccgctc ctactattac gcgatggggc
    10021 tcacgcgcga tcagatccac cagcccttcg tcggcgtcgc cacctgctgg aacgaggcgg
    10081 ctccttgcaa catcgcgctg atgcgccagg cgcaagcggt caaggccggt gtgatcgccg
    10141 ccaacggcac gccgcgcgag ttctgcacca tcaccgtgac cgatggcatc gccatgggcc
    10201 atcagggcat gaaatcctcg ctgccgtcgc gcgaagtgat tgccgacagc gtcgagctta
    10261 ccatgcgcgg ccattgctac gacgcgctgg tcggcgtggc gggctgcgac aaatctctgc
    10321 ccggcatgat gatggcgatg tgccggctca acgttccctc catcttcctc tatggcggca
    10381 cgatcctgcc cggctcgttc aggggccgcg ccgtcaccgt gcaggatctg ttcgaggccg
    10441 tcggccaata ctccgtcggc aagatgtcga cggaggatct cgacgagctt gagcaggtgg
    10501 cctgcccatc ggcgggggcg tgcggggcgc agttcaccgc caacacgatg gcctgcgtct
    10561 ccgaggccat gggtctcgcg ctgccatact cggcaggcgc gcccgcgccc tacgagatcc
    10621 gggaccggtt ctgccaggcg gcgggcgaaa agatcatgga cctcatcgcc ttgaatatca
    10681 ggccgcgcga catcgtcacc cgcgaagcgc tcgagaacgc cgcggctgtc gtggccgcga
    10741 ccggcggctc aaccaatgcg gcgctgcatc tgccggccat tgcgcatgaa tgcggtatcg
    10801 acttcgactt gttcgccgtc gccgatgtca tgaaacggac accttatgtc gcggatttga
    10861 aaccgtccgg ccgctatgtc gccaaggact tgtttgaagc tggcggcgtg ccgctccttc
    10921 tcaagaccct tctcgatcat ggctatcttc acggcgaggg catgaccgtg acgggccgga
    10981 gcctgaaaga caatctcgcc aaggtgaagt ggaatccgga tcaagacgtc gtgcggccgg
    11041 ccgacgatcc gatatcgccg accggcggcg tggtgggctt gcagggctcg ctcgcgccgg
    11101 acggggccat cgtcaaggtg gccgggatga agcggctgaa attccgcgga cccgcccgct
    11161 gcttcgattg cgaggaggac gccttcgccg cagtcagcgc caagcgctac aacgaaggcg
    11221 acatcatcgt catccgctac gagggcccga aaggcggccc cggcatgcgc gagatgttgt
    11281 cgacgacggc ggcgctttac ggccagggca tgggcgacaa agtggccctc atcaccgacg
    11341 gccgcttctc cggcgccacg cgcggcttct gcgtcggtca tgtcgggccg gaggcggctc
    11401 ttggcggacc gatcgcgctc atccgcgacg gcgacatcat tgcgctcgat gccgagaagg
    11461 gcaccctcga tgtcgagctc gacgcgagcg aactgaagaa gcgcgccaag gcgtggacgg
    11521 cgccgccgat gcgtttcaag agcggcgccc tgtggaagta tgcgaacgag gttggcccgg
    11581 cgcggaccgg tgccgtgacc catccaggtg gtggcgtgga agttgcgtgc tatgcagatg
    11641 tgtaacaggt tggcgttggg ggcgctggcg gcgctcctga cgatgggccc gatgatgggc
    11701 cccgtggcgg tcggcgccga tgatccgccg ccttatcctt cggctaccga ggcttatcgg
    11761 cagggcgtga aggcgctcaa ggcgggccgc ccggtgacgg cggtgcctgc cctcgaatat
    11821 gccgccaaac gcggcgtgct gggagcccag gtcaaacttg cgcgcggcta cgcgtcgggc
    11881 cgggacgtgc caaaggacga cgccaaggcc ttcgcctatt tcgagcagat cgccgaccag
    11941 caggccgaca tcagcccgtc gagtccggtc gcgaaatatg ccgctgaggc gttcgtggcg
    12001 ctcggccagt attacctcga cggcatcccg ggaaagccgc tcgaggccaa cccttcctat
    12061 gccgcggatt tgttccggca cgccgcgagc tatttcggcg acgccgaggc gcaataccgg
    12121 cttgcgcgcc tctatatgaa cggcaccggc gtcgagaaga atgtgggact tgccgtcaat
    12181 tggcttgcga ccgccgccaa gaagcagcac gcggcgtcac aagcgacgct cggcgagatt
    12241 ctgtggcgcg gccaggatgt gcgcgaaagg cgggcgcgcg gtctcgcgtt gattatcctc
    12301 gcgcacgaga atgcgcgagc cggcggcaaa gagccgcaat ggatcggcgc gctctacgac
    12361 gaagcgtttg ccaagtctga caaagcgacc cgtaaggatg cccaggcgct cctgccggca
    12421 ctgagcagcg tcaaggcgac atcggttttg ccctccgagg cgaagccttc cgaaatcttc
    12481 gtcatgccgg ctcaggagaa agcgcaagcg cctgggatgc cggcttccga taggtccggt
    12541 ccgggtgctc cgcagcctgc tccgatcggg atgtccgtcg gcttcggcgg caatcgcggc
    12601 gaagcgggct tcaagcctta gggtcccccg ccgcaaccgg acgcgccgct caaagcgcga
    12661 gttccgccac gattggcacg tgatcggagg gcttctccca gctccggggg cccgcatcga
    12721 tctcgcatgc ctgaagccgg tccgccgcct gcggcgacag caatacatgg tcgatgcgca
    12781 agccctgatt cttctggaag gcgccggcct gatagtccca gaacgtgtat cgatgcggct
    12841 cgtcgtgaca ggcgcggaag gcatccgtca gcccgagatt gaggagcgcg tggaaatgag
    12901 cgcgggtctc gggacgaaag agagcatcgt ccatccacac actcggattg tagacgtcct
    12961 ccggcgccgg gatcacgttg aagtcgccgg cgatgatgag cttctcctcg ttggcgagca
    13021 gggctctcac acgctccttg agccgggtca tccaggcgag cttatagacg aacttctcgg
    13081 tgccgaacgg attgccgttc ggcagataga tattgatgag gcggagcgcg cccgagggga
    13141 ctgagatcac cgcttcgaga tagcgcgcct gtacgtcgtc gggatcgccg gcgagccgcg
    13201 ggtgaacctc gtcgaacggg agcttcgaca gaagcgcgac cccgttgaag cccttctgcc
    13261 catgcaccgc gacgttatag ccgagctcct cgaactgctc cttcgggaac gcctcgtcga
    13321 cgcatttgat ctcctgaaga caggcgatat cgggcgaggc ggccttgagc caggtcgttg
    13381 ccgcctcgat gcgcgccttg ataccgttga tgttccagga ggcgatcttc atggatggat
    13441 tgctgaatgc tcgaactgac gggcaattcc agtagcccaa gcctgccaaa gcatcaaatg
    13501 cgcgcgcata ttgcgtgcct tttcaaaagt ccccctcgtg gggaggtcga cggcgccgaa
    13561 ggcggcgtcg gggtgggggg tttgcgcttc aagtagcaag gcgatcgcga gtggaaagac
    13621 catcacctca cccagctcgg cctttggcct cgccgccctc cccatcaagg ggagggtttg
    13681 tttttgcgtt tgcaggctca tccgtcattc cggcgaaagc cggaatccag aagcggcaag
    13741 cgctgcgttt gtggtcctgg ataccggctt tcgccggtat gacgcggggt aagctaaatc
    13801 gagaaactgg tgccgcagcc gcacgcggcg gtcacgttcg ggttgttcag cttgaactgg
    13861 gcgccgatca gatcgtcgac gaaatcgagc tcggcgccgt gcaggaagcc gagcgagacc
    13921 ggatcgacga tcacccgcgc gccgtcccgc tcgatcagga gatcgtcggg ggcgcgctcc
    13981 gtcaccaggt cgaagcggta ttggaaaccg gagcagccgc cgccctcgac gctcacgcgc
    14041 aacagcgaaa gcgccgcctc gcccttgagg atctcggcga tgcggttcgc ggcgcggtcg
    14101 cttatggtga tggacgcttc gctcatcttg ggtgtagcca atctctcacc gaaagatagg
    14161 gtggtacggg cgcttgtcaa tcgaccacgg acgcaaggcc gaggggactt tcgcggcaag
    14221 gccggcaagc ggcgacctta cggcggaggc agcccctgcc cgcgtccctt acgccgtcga
    14281 tccgagcgca agccgcgggc gccttcagcc cgagccccca cgcgacacgc gctcgcctta
    14341 tgcccgcgac cgcgaccgta tcctccatgc cacggccttc cgccggctca actacaagac
    14401 ccaggtcttc gtctatcatg aaggcgatca ctaccgttcg cgcctcaccc acagcctcga
    14461 agtggcgcag attgcccggg cgcttgcccg cacgttgcgc ctcgacgagg atctcaccga
    14521 ggcgctcgcg cttgcccacg atctcggcca tccgcccttc ggccatgccg gtgagcgcgc
    14581 gctcgatgcg gcgatggaag gttccggcgg cttcgaccac aatgcccagt cgctccgcgt
    14641 ggtcgccgcg ctcgaaaaga aatatgcgcg cttcgatggt ctcaatctga cctgggagac
    14701 gcttgaaggc ttggtcaagc ataacggccc gctgaccggg ccccatgcgc cgcggcagcg
    14761 cgcggtgccg gagccgatcc gcgactactg tgccaagcag gacctcgaac ttgaaacctt
    14821 cgcctcggtc gaggcgcaag ccgcctcgct cgccgacgac atcgcctata acaatcacga
    14881 tatcgacgac ggataccgcg cagggttctt caccttcgcc gaacttgcgg aagtgccgat
    14941 cgccgcgcgc gcactcgccg aggtgcggga agcctatccc ggcatcgacg gcacccggct
    15001 cctctatgaa gccattcgtc gcctgatcgg cgcgatgatc gaagacgccg tcgccgaaag
    15061 cgagcggcgg cttggcgcgc ttcagccctt aagcgccgcc gatatccgcc acgcgccgca
    15121 cgccgtcgtc gccttctccg aagagatgcg ccgcgacctc gatggcttgc gcgcctttct
    15181 tttcgcccgc gtctaccgcc atgagcgcat cgttcgcatc atggccgacg ccgagggcgt
    15241 ggtgcgcgac ttgttccggc gctatagcgc cgagcccgag acgctcccgc ccgactggcg
    15301 gggaccggcc ccgccttgcg gcacgcgcgc ctatgcccgc cacgtcgc
//
LOCUS       DASUQP010000158         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_624537,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000158 DASUQP010000000
VERSION     DASUQP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624537"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /gene="lon"
                     /locus_tag="VFY74_07000"
     CDS             <1..1487
                     /gene="lon"
                     /locus_tag="VFY74_07000"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5931481.1"
                     /translation="IELPWSTLSDDRIDIAEARRILDEDHYGLPKIKQRILEYLAVRK
                     LKPEGKSPILCFVGPPGVGKTSLGQSIARAMGRKFVRLSLGGVHDEAEIRGHRRTYIG
                     ALPGNVLQSIRKAGTRNPVMMLDELDKLGASFHGDPSAALLEVLDPEQNSTFRDNYLA
                     VPFDLSRVMFIGTANVLDQIPGPLRDRMEVIELPGYTEEEKVEIAKRYLVKRRLEATG
                     LTPEQCEIEEDAIRAIIRDYTREAGVRNLERDIGALCHHVAMRIAEGDTMRMRIGADQ
                     LHAILGPRRFESEVAMRTSVPGVATGLAWTPTGGDILFVEAARVPGTGKLILTGQLGE
                     VMKESAQAALSLVKARASMLGIDPASFEKSDIHVHVPAGAIPKDGPSAGVAMFVALVS
                     LLTGRTVRSDTAMTGEISLRGLVLPIGGVKNKVLAAVRAGITIVLLPERNKKDFEDVP
                     ETARKAVHFVWLSTVDDAIEAALETVPQSETLAASDEQRRAASG"
     gene            1690..2433
                     /locus_tag="VFY74_07005"
     CDS             1690..2433
                     /locus_tag="VFY74_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX5931482.1"
                     /translation="MRQPSITAEILSGDLRRLGVRGGDTLMIHVSLRALGRIDGGPDA
                     LLDALEAAVGDDGTLLMILGAEIAEDWVNRLPELERSSLLAGAPPFDPATAPVFHEVG
                     YFAEVFRRRRGTLVTDNPSGRFGARGCLAHALLAGAPWHDYYGPGSPLDRLCRHGGRV
                     LRIGANLDATTVLHFAEYRAEVGAKRRVRPHYRCLGPEGPETRAVECLDDEHGIVEWP
                     GEDYFATILRAYLATGRAKEGHVGLARVS"
     gene            2662..2859
                     /locus_tag="VFY74_07010"
     CDS             2662..2859
                     /locus_tag="VFY74_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931483.1"
                     /translation="MRLLFGMILGAVLTVAAAYIADSRSDPLQGTRMVNWNVVGEKVN
                     ELTVDLRRLWRDFTRQITGPP"
     gene            complement(2856..3440)
                     /locus_tag="VFY74_07015"
     CDS             complement(2856..3440)
                     /locus_tag="VFY74_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931484.1"
                     /translation="MRQHPTTPAAPRAQQPTTPAAPRIDPILIERERNRLGARALGFL
                     IVLNGAAAIVLLSILARAPEASVDSKMAAALLFFSGGAIAALLSSFLAYINRTVALEA
                     PERANLRRGLQIVSIVAVIGSAIAFLTGMNMVAAAASERSSSHPKGPKEQQRPKGDSA
                     PTAQPSERVEVPKPIGGSAEIGPLLLASARREPA"
     gene            complement(3743..4153)
                     /locus_tag="VFY74_07020"
     CDS             complement(3743..4153)
                     /locus_tag="VFY74_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5931485.1"
                     /translation="MPVDQRDAWVRAYLEEVFNGHDLRSLDKYMSENLVSHWLGDRSL
                     RGREAWREAMANFFDAFPDATYTLNDLFFAGDKGVWRGTWDATQQKRWEGISATGRKA
                     KWTVIIIGRFEGDKLAEDWIEYDRYNLFRQLGAL"
     gene            4299..4760
                     /locus_tag="VFY74_07025"
     CDS             4299..4760
                     /locus_tag="VFY74_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX5931486.1"
                     /translation="MIRKLAFVVGCALIQTLSNPAFVAAAEFGTAEEAKAMLERAVAA
                     VKEDKAKALDMFNKGEGGFKDRDLYVWCANASDGIISATPYWNRGKQVRDIKGKHGAP
                     FGETIMQDATEGTIKETTYWWPRPGTDKPLEKTFFYTKVADQICGVGYYKE"
BASE COUNT          991 a         1661 c         1718 g         1007 t
ORIGIN      
        1 tgatcgagct tccctggtcg acgctctcgg acgatcggat cgacattgcc gaggcgcgcc
       61 gcatcctcga cgaggaccat tacgggcttc ccaagatcaa gcagcgcatc ctcgaatatc
      121 tcgcggtgcg caagctgaag ccggaaggaa agagcccgat cctctgcttc gtcggccctc
      181 cgggcgtcgg caagacctcg ctcggccaat cgatcgctcg cgccatgggc cgcaaattcg
      241 tgcggctcag cctcggcggc gttcacgacg aggccgagat caggggccat cgccgcacct
      301 atatcggggc gctgcccgga aacgtgctac agtcgatccg aaaagcgggc acgcgcaatc
      361 ccgtgatgat gctcgacgag ctggacaagc tcggtgcgag cttccacggc gatccgtctg
      421 ctgctctcct cgaggtgctc gatcccgagc agaacagcac gttccgcgac aactacctcg
      481 cggttccgtt cgacctgtcg cgcgtgatgt tcatcggcac ggcgaacgtg ctcgaccaga
      541 tccccgggcc tttgcgcgac cgcatggagg tgatcgagct ccccggctac accgaggagg
      601 agaaggtcga gatcgccaag cgctatctcg tgaagcgccg gcttgaagcg acgggcctca
      661 cgcccgagca gtgcgagatc gaagaggacg ccattcgcgc catcatccgc gattacacgc
      721 gcgaggccgg cgtgcgcaat ctggagcgcg acataggcgc gctctgccat cacgtcgcca
      781 tgcgcatcgc cgaaggcgac acgatgcgca tgcggatcgg cgccgaccag ctgcatgcga
      841 tcctgggacc gcgccgcttc gagagcgagg tggcgatgcg cacctccgtg cccggggtcg
      901 cgaccgggct cgcctggacg ccgaccggcg gcgatatcct gttcgtcgag gcggccaggg
      961 tgccgggaac cggcaagctc atcctcaccg gccagctcgg cgaggtgatg aaggaaagcg
     1021 cgcaagcggc gctcagcctg gtcaaggcgc gagcgtccat gctcggcatc gatcccgcct
     1081 cgttcgagaa gtcggacatc cacgtgcatg tgcccgccgg cgccatcccc aaggacgggc
     1141 cgagcgcagg cgtcgccatg ttcgtggcgc tcgtctcgct tctcacgggg cgcaccgtgc
     1201 ggagcgacac ggccatgacc ggcgagatct cgctgcgcgg gctcgttctg ccgatcggcg
     1261 gcgtgaagaa caaagtgctg gcggcggtgc gcgcgggcat caccatcgtg ctcctgcccg
     1321 agcgcaacaa gaaggatttc gaggacgtgc ccgagaccgc gcgcaaggcc gtgcacttcg
     1381 tctggctgtc gaccgtcgac gacgccatcg aggccgcgct cgagaccgtg ccgcagtcgg
     1441 agacgctcgc cgcctccgac gagcagcgcc gcgcggcgag tggctagcca cgagtggcta
     1501 gcccgaggtc gagtcgcagg cggctagagc atcgaattca atcgtgaccc ccctcttatt
     1561 cccccctttc agggggtatt aaaattggag cgggtattcc tctccctccc cctgcaaggg
     1621 agagggccgg ggggggtcgg gagccgtggt tcaatccggc tgaataatgc tctagaagcg
     1681 agccgttcgg tgcgccagcc gtctatcacc gccgagatcc tgtccggcga tcttcgccgc
     1741 ctcggcgtgc gcggcggcga cacgctcatg attcacgtgt ccttgcgtgc gttaggccgc
     1801 atcgacggcg gccctgacgc tttgctcgat gcgttggagg cggctgtcgg cgatgacggc
     1861 acgctcctga tgatccttgg cgccgagatc gcggaagact gggtgaatcg gctaccagag
     1921 ctagagcgat cttctctcct cgccggcgcg ccgcccttcg atcccgccac ggcccctgtt
     1981 ttccacgagg tcggctattt tgccgaagtc tttcgccggc gccggggtac gcttgtcacc
     2041 gacaacccga gcggccggtt cggcgcgcgc ggatgcctcg cacacgctct gctcgccggc
     2101 gctccctggc acgactatta cggacccggc tcgcccctcg accgcttgtg ccggcacggc
     2161 gggcgcgtcc taaggatcgg cgccaatctc gacgctacga ccgtgttgca cttcgccgaa
     2221 tatcgcgcgg aagtcggtgc gaaacgccgc gtgcggcccc actatcgctg cctcggccct
     2281 gaaggcccgg agacgcgcgc ggtggaatgc ctcgacgacg agcatgggat cgtcgagtgg
     2341 ccgggcgagg attactttgc caccatcctg cgcgcgtatc ttgcaaccgg ccgggcgaaa
     2401 gaggggcacg tgggtcttgc cagagtgagc tgatcgcggc aggcgacctc gtcgatttcg
     2461 gcacgcaatg gatgaacgcg cgattcgggg gcctccaggc gaatcaggcc tgaaggatgt
     2521 gggcgaggat ccgggccagt atgctcgcgc ctggcctctc agtggcattt atgcacagtt
     2581 ccacggctgg cgttggcatg cttccaactt cctcttcgct gcgcggacgc ttatgattgg
     2641 gagttcttga gaggacgggg gatgcgactt ctctttggca tgatcctcgg cgccgtgctc
     2701 acggtcgccg cggcctatat cgccgattca agatcggacc ctctccaggg gacccggatg
     2761 gtgaactgga acgtggtcgg cgaaaaggtg aacgaactca ccgttgactt gcggaggctc
     2821 tggcgcgatt tcacgcgcca aatcaccggg ccgccctaag cgggctctcg ccgcgcggat
     2881 gcgagcagca aggggccgat ttcggcgctg ccgccgatgg gcttcggaac ttcgacccgc
     2941 tcgctcggct gggccgttgg tgcgctgtct cctttagggc gctgttgctc cttcgggcct
     3001 ttgggatggg agctcgaccg ttccgacgcg gcggccgcta ccatgttcat gccggtgagg
     3061 aacgcgatgg cgctgccgat aacggcgacg atgctgacga tctggaggcc gcgcctgagg
     3121 ttcgcgcgct cgggtgcctc cagggcaacc gtcctgttga tgtaggcgag gaacgaactc
     3181 aagagagcgg caatggcccc gccgctgaag aacagcagtg cagccgccat cttgctgtcg
     3241 acactcgctt ccggcgccct cgccaagatc gagagcagga cgatcgcggc cgcgccgttg
     3301 agcacgatga gaaagccaag cgcccgggcg ccaagccgat tccgctccct ctcgatgagt
     3361 atagggtcga tgcgcggagc ggcgggggtg gtgggttgtt gggcgcgcgg agcggcgggg
     3421 gtggtggggt gttggcgcat gcgcgtcata aatcctgagc ttggggccgc attgtgaccg
     3481 cggcgatccc atctgcgtcc accgaaagtt tttccgaaac cgggcctggt ggccggtccc
     3541 ctgccgcaag gaaccggcca gagcgccaat ggtagcaggc ctaggccgct tccaatctcg
     3601 ccaattcggc ctcaaattca aagcgcaatt gcgcgccttc gtcccggaag aggtcgcgct
     3661 gacgagaaca catatctcag cctttttgga gccgaatcac gcaaatcggc gggtcgcacc
     3721 aagtggttca tgtacagata tttcagagcg ccccaagctg ccgaaacaga ttgtatcggt
     3781 cgtattcgat ccaatcttcc gcgagcttgt cgccttcaaa ccggccgatg atgatcacgg
     3841 tccacttcgc cttgcggccg gttgcggaaa ttccctccca ccgcttctgc tgcgtggcat
     3901 cccaagttcc ccgccacacg cctttgtcgc ctgcgaagaa gaggtcattc aaggtgtacg
     3961 tggcgtcggg aaaagcgtcg aaaaagttgg ccatcgcctc tctccaggct tcccgcccgc
     4021 gcaggctccg gtcacccagc cagtgcgaga cgagattttc agacatatac ttgtcaagtg
     4081 atcgcaggtc atgaccgttg aacacctcct cgagataggc acgaacccat gcatcacgct
     4141 gatctacggg catcaccaaa tctccttttt ttgagcaagc ggcgaagact gatgcgactt
     4201 cgcggcaacg tcagcggcaa tgattgatgg tactatgcgt ctcatcgagg gaatcggggc
     4261 caaataaaat acgtgttttc gtccccatgg aggaagtcat gatacgtaaa ctcgcattcg
     4321 tcgtcggttg tgccctcatt cagacacttt ccaatcccgc cttcgttgcg gcagctgagt
     4381 tcggcaccgc agaggaagcc aaagcaatgc ttgagagagc agtggccgca gtgaaagagg
     4441 acaaggcgaa ggcgcttgat atgttcaaca agggcgaggg cggctttaag gaccgtgatc
     4501 tttatgtgtg gtgcgccaac gcctcggacg gcatcatatc ggcgacgcct tactggaata
     4561 ggggcaaaca ggtgagagac atcaagggca agcacggtgc tccgttcggt gaaacgatca
     4621 tgcaggatgc caccgaggga acgataaaag agacgaccta ctggtggccg cgccctggca
     4681 cagacaagcc gctcgaaaag accttcttct acacgaaggt cgctgaccag atttgtggtg
     4741 tcggctacta caaggaataa cttgccgggc acctggtgtc cgctatggga caaagcggac
     4801 accgcgatcg aaccgatagc tgtcagccgc tccgcgcctg gcaggtgcag gcttagcgct
     4861 cacgactctg gatctgagcc gcttgttatt cggctcgtcc atctctctgg acttccgttg
     4921 tggcagggag ttttcgacag tagcttgagg gtcgtgtcac tctgctcgga acctgcaact
     4981 tagggcgtgg cgcgcatggc gaaagcgatc gcaaagcacc ggaaaacctc atcaagtcga
     5041 cctaggcaag ccacgagcgg cccgctatgc gatcgacgtg ctgcgcgcgt tcggtggcgc
     5101 gatcctattt gctcgagctc catagaggca ctcttgctcg agtccagcga tcgaccagga
     5161 aatgggtgcg cagtctttaa taagttggcg gagacggaga ccgtttggtt tgggtccagg
     5221 tccgtcccag atcgtccaat ttgccagttg gttgtctgaa cggtaacgct ttttgcttct
     5281 agacgcgtcc gaattcgtct acgacaatcc agcttcggaa tgtgggtaga cgggtgggat
     5341 gatgcgggaa gccaacaagc tttcagcggt gaaggtg
//
LOCUS       DASUQP010000159         5253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1305812, whole genome shotgun sequence.
ACCESSION   DASUQP010000159 DASUQP010000000
VERSION     DASUQP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305812"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1695
                     /locus_tag="VFY74_07030"
     CDS             277..1695
                     /locus_tag="VFY74_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX5931487.1"
                     /translation="MLRPIALALALTAANFAPALSETASLTEEEAQAIGVDAYLYFYP
                     LITMDLTRKQLTNQEPTPGGIGGPMNRFSNIGAFPTADMKVVVRPNFDTLYSSGWLDL
                     TKEPVVVSAPDTGGRYYLLPMLDMWTDVFASPGWRTTGTQAANFLVTPPGWSGTVPDG
                     FTRIDAPTPYVWVIGRTKTDGPADYDAVHKIQAGYKVTPLSQWGKEPAAPEVKIDPSI
                     DMKTPPKTTVDTMKAGPFFAYAAELLKLHPPHITDQPIIARLKRAGFEVGKSFDLDTA
                     DPAVKKGFEGAPETAQKLMAWKLPTIARVTNGWSMNTDTMGVYGNYYLKRAIVTQLGL
                     GANLPADAVYPLNLGDDSGKPLDGASNYTIHFDKGQTPPTDAFWSITLYDNDGFQVAN
                     ALNRFAVSSWMPFEYNPDGSLDLYFQNESPGADKEANWLPAPKGPFTLTMRIYAPRGD
                     ALTGKWNPPPVTKVHTVPASAQ"
     gene            complement(1723..2055)
                     /locus_tag="VFY74_07035"
     CDS             complement(1723..2055)
                     /locus_tag="VFY74_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5931488.1"
                     /translation="MRLQAALILILLSTPACAGQKVTATWYGAELAGNRTASGERFNP
                     NGLTAAHRSLPFGTCLRVSNPKTGRSVAVRVNDRGPFTKGVSLDLSHGAARAIGMRVT
                     QPVIVDRC"
     gene            complement(2204..4258)
                     /locus_tag="VFY74_07040"
     CDS             complement(2204..4258)
                     /locus_tag="VFY74_07040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5931489.1"
                     /translation="MIFAVLRRPPNGEIEAGSAWWRRALRLAISVLVLPALGLAIAFT
                     LMKIAAQTDYWDLPGGRLLDATLSSCQFEHLNLAGIDVEHMICPTRLAAGAFPQVLKD
                     AVIASEDKRFFSHGAIDIPSTARAAWHFLLGDRRGGSTLTQQLARTLLLKKEDRIERK
                     LLEGVLAIRIFARRTRAEIHALYMNVVPHARNMYGFDDPARYYFGVGVQELTLGESAL
                     LVGMLPEPNDRDPLKSPDAAVKSAVGVLQRMVAEKMITVEQAARAENELKRRVRNGTL
                     RRGDQVYRRLEYRPYRDLALREAEANGIKLPADYRLFVFIDPGFQRILQTQICSITGP
                     HQGAGVFMRPSGEILALTGSCTYTGAWNRAADIARSIGSAGKLFTLVGVHEAGISLQQ
                     RFSTWPLTRSGWPAEPNPLCRKRAKVSLAFALVQSCNRPWTEVAMRLGPRVIDIVKRF
                     DVPPPSTPSLVPIGGVQISPLKLSQAYAALANEGKLPQVRSLIAAIGPRGNILGLPAT
                     KQTPKVMSSATASAILKSLRGPVKHGTARAANSVHALVYGKTGTSSRNVDALFVGLTQ
                     DFVGSIWLGRDRPAPMPGIHGGGMPARTFAKLTDFYYVRLAQARFGETEASPWDRFGR
                     FASGQSAIKILTALGSLLMTWLLITAVFRRKQRAVGAEQVAEPSVEVLGVSEPSENPW
                     RT"
     gene            complement(4430..4864)
                     /locus_tag="VFY74_07045"
     CDS             complement(4430..4864)
                     /locus_tag="VFY74_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5931490.1"
                     /translation="MHINLENAEQAIEAARKKAAALKTQMCIAVVDSGANLKAFLRMD
                     DAWVGSIDIAIKKAKTACFFGMATGQLGKISQPGKPLYGIEHSNDGLITFPGGLPIVD
                     EEGVLVGAIGVSGSSVENDHKVAEAGVKVLGVSELPEHPWRT"
     gene            complement(5008..>5253)
                     /locus_tag="VFY74_07050"
     CDS             complement(5008..>5253)
                     /locus_tag="VFY74_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5931491.1"
                     /translation="SPVLGVIAKGVELVVLDRKRGWVQVTDPASGKKGWIYSGLLAGE
                     AKTQRRVRRGAPAEADSNSAPESESFWGRVGRWLTPG"
BASE COUNT         1021 a         1657 c         1618 g          957 t
ORIGIN      
        1 gtatgctgac gatctctcca gatcggcgct cgaggggtta cgaatctccg caactcaaag
       61 cgcagactcc atctgaagtg acgcgggcta aagcgctgta atgcatcgtc ccgtatacgg
      121 aggagttaga tcgtgctgcg cggcatgcca taaccacgcg gcgcccgacc aggcagctct
      181 tgtgtcgcgt gcgagaagcg acaacctgtt ggaagcacgc gaccgattgc ctcgtcaatg
      241 gcttcctaaa gagttggtac cttgagggga taaaaaatgt tgagaccgat cgctttggca
      301 cttgcgttga cggcggcaaa tttcgcgcct gccctatcgg agaccgcgtc gctgaccgag
      361 gaggaggcgc aggccatcgg cgtcgacgcc tatctctatt tctatcctct gatcacgatg
      421 gacctgacgc ggaagcaact caccaatcag gaaccgaccc ctggcggcat cggcggcccg
      481 atgaaccggt tctccaatat cggggcattt cccacggccg acatgaaggt cgtggtcagg
      541 ccgaatttcg atacgctcta ttcgagcggc tggctcgatc tgacaaaaga gcccgtggtc
      601 gtttcagccc ccgataccgg cggacgctat tatctcctgc ccatgctcga catgtggacc
      661 gatgtgttcg cctcgccggg ctggcgcacg acgggaacgc aagcggcaaa cttcctggtg
      721 acgcctccag gctggagcgg gacggttccc gacggcttca cccgcatcga cgcgccgacg
      781 ccttatgtgt gggttatcgg ccgcaccaag accgacggtc ctgccgacta cgacgccgtg
      841 cacaagatcc aggccggtta caaggtcacg ccgctgtcgc aatggggcaa ggagccggcg
      901 gcgccagagg tcaagatcga cccctccatc gacatgaaga cgccgccgaa gacaaccgtc
      961 gatacgatga aggccggacc gttcttcgcc tacgccgccg agctcctcaa actgcatccg
     1021 ccgcacatca ccgaccagcc gatcattgcg cggctgaagc gcgccgggtt cgaagtcggc
     1081 aagagcttcg atctcgacac ggcggacccc gccgtcaaga aaggcttcga gggcgcgccg
     1141 gaaacggcgc agaaactgat ggcgtggaag ctgccgacca tcgccagggt caccaatggc
     1201 tggtcgatga acaccgacac catgggcgtc tacggcaatt attatctgaa gcgcgccatc
     1261 gttacgcagc tcggcctcgg cgccaatctg cccgccgatg ccgtctatcc gctcaacctc
     1321 ggcgacgaca gtggcaagcc gctcgacggc gcctcaaact acacgatcca tttcgacaag
     1381 ggccaaacgc cgccgaccga cgcgttctgg tccatcaccc tttacgacaa cgacggcttt
     1441 caggtcgcaa acgccttgaa ccgcttcgcc gtgtcgagct ggatgccgtt cgaatacaat
     1501 cctgacggat cgctcgatct ctatttccag aacgaaagtc ccggagccga caaggaagcc
     1561 aactggctgc cggcgccgaa aggccccttc acgctcacca tgcgcatcta tgcgccgaga
     1621 ggcgatgcgc tgacgggaaa atggaacccg ccgccggtga ccaaggtgca caccgttccg
     1681 gcctcggcgc aatagcgctg cggctgctgc ggctcctatt cgctagcacc ggtcgacgat
     1741 cacgggctgc gtaacgcgca tgccgatcgc gcgggcggcg ccatgcgaca gatcgagcga
     1801 cacgcccttc gtgaacggcc cgcgatcgtt gacccgaacc gccacgctgc gacccgtctt
     1861 cggattgctc acgcgaaggc aggtgccgaa cggcagagaa cggtgggcag ccgtaagccc
     1921 gttagggtta aagcgttcgc ccgatgcggt gcgattgccg gcaagttccg ctccatacca
     1981 ggtcgccgtc accttctgcc ccgcgcaggc aggcgtggag aggaggatga gaatgagtgc
     2041 agcctgaagt cgcatgagct cgctccgatt gtttgatgtg atcggcgctc gcgtagcctt
     2101 cgaatgcggt cattgatcgg cgtcaatgcg acctgttttg gcgagagttt gaccatttcg
     2161 ggaggtgcag agcgccgagc accaatccgt agcgacgtgt tccttatgtg cgccacggat
     2221 tttcggacgg ctcgctcacg ccgagcacct ccacgctagg ctcggcgact tgctccgctc
     2281 cgaccgctcg ctgcttcctg cggaacacag ccgttatcag caaccatgtc atcagcagcg
     2341 agccgagagc ggtcaggatc ttgatcgccg actgtccgga ggcaaatcgt ccgaatcgat
     2401 cccatggaga cgcctccgtt tcgccgaaac gcgcttgagc aagcctgacg tagtagaagt
     2461 cggtcagctt tgcgaaggtt cttgccggca tgccgccgcc atgaataccc ggcatcggcg
     2521 caggacggtc gcgcccgagc catatgctgc cgacaaagtc ctgagtgagg ccgacgaaca
     2581 gcgcgtccac gttgcgcgat gaggttccgg tcttgccata aacgagcgcg tggaccgagt
     2641 tggcggcacg tgccgtaccg tgtttgaccg ggccgcgtag gcttttgagg atggccgacg
     2701 cggttgcgct cgacatgacc ttcggcgttt gcttcgtcgc cggcagaccc aagatgttgc
     2761 cccgtggccc gatcgccgca atcaaggacc tgacctgcgg cagcttgccc tcgttggcaa
     2821 gcgccgcgta ggcctgcgag agcttcagag gcgatatctg cacgccgccg atcggcacga
     2881 gcgagggcgt acttggcggt ggaacatcga accttttgac aatgtcgatc actctcggac
     2941 cgagccgcat tgccacttcg gtccaaggcc gattgcagga ttggacgagc gcgaacgcga
     3001 ggctgacctt ggcccgcttg cggcaaaggg gattgggttc tgccggccag ccggatcgtg
     3061 tcagaggcca ggtcgagaac cgttgctgca ggctgatgcc cgcttcatgc acgccgacga
     3121 gcgtaaagag ctttccggcc gatccgatcg agcgggcaat gtcggcggcg cgattccacg
     3181 cccccgtata ggtgcaggag ccggtcaggg ccagaatttc gccgctgggc cgcatgaaga
     3241 ctccggcgcc ctgatgcggc ccggtgattg aacaaatctg cgtctggagg atgcgctgga
     3301 acccaggatc gatgaaaacg aagagcctgt agtcggccgg cagcttgatg ccgttcgctt
     3361 cggcttccct aagagccaaa tcgcggtagg gccggtattc aagtctgcga tagacctggt
     3421 ctccacggcg aagcgtgccg ttccggacgc gacgcttgag ctcgttttcg gcacgcgccg
     3481 cctgctccac cgttatcatc ttctccgcaa ccatgcgctg aagcacgccg acggcgctct
     3541 tgacggcagc gtccggcgac ttcaggggat ctcgatcgtt tggctccggc aacatgccga
     3601 cgagcaacgc ggactcgccc agcgtcagct cctgcacgcc aacgccgaag tagtaacggg
     3661 ccgggtcgtc aaatccgtac atgttccgcg cgtgcggcac gacgttcatg tagagcgcgt
     3721 gaatttcggc gcgcgttcga cgggcaaata tcctgatggc cagaaccccc tcgaggagct
     3781 tgcgctcgat tctgtcctcc tttttcagta atagcgtgcg ggcaagctgc tgggtgagcg
     3841 tgcttccgcc tcggcgatca cccaggagaa agtgccaggc ggctcgcgcg gtggacggaa
     3901 tgtcaatcgc gccgtgcgag aagaagcgct tgtcctcgct ggcaatcacg gcgtctttga
     3961 gtacttgcgg aaacgcgccg gcggccaacc gcgtcgggca gatcatgtgt tcgacatcga
     4021 taccggccag gttcaaatgc tcgaactgac aggaagatag ggtcgcgtcg agcaagcggc
     4081 ctccgggcag atcccaatag tcggtttggg ccgctatctt catcagagtg aaggcgatgg
     4141 ccaggcctaa agccggaagc acaagaacgc tgatcgcgag gcgcagagcg cgccgccacc
     4201 aggcagagcc cgcctcgatc tcgccattcg gtggccgccg cagaaccgca aagatcacga
     4261 ccaaatccca acgctacgtt tgagagggcg gttgggcggc gccgaccgcc cacgcctcac
     4321 ccgggcctat ataggtctgc cggccaattg cggcgcgcaa gtgatcttgc ggcgcgcgaa
     4381 tgggataaaa agggccgaaa ccggcgcggg ggccgaggga ttcgaccttc taagtccgcc
     4441 acggatgctc gggcagctcg ctcacgccga gcaccttcac gccggcctcg gcgaccttgt
     4501 ggtcgttctc gaccgagctg ccgctgaccc cgatggcgcc gaccagcacg ccctcctcgt
     4561 caacgatcgg cagaccgccg ggaaacgtaa tgagcccgtc attggagtgc tcgatgccat
     4621 agagaggctt gcccggctgg gaaatcttgc cgagctggcc tgtggccatt ccgaagaagc
     4681 aagcggtctt cgccttcttg atggcaatgt cgatggaccc cacccaggcg tcgtccatgc
     4741 ggagaaaggc cttcaggttg gcgccggaat cgaccacggc gatgcacatc tgcgttttca
     4801 gcgcggctgc cttcttgcgt gcggcttcaa tcgcttgctc cgcattttcc aaattgatgt
     4861 gcatgatgtt tctcctggtt ttgggggaat taggggcagg aaaagggctt gcaaccgggt
     4921 gttaaagacg catgacggtc agatgttcca gagatgcggc gcgcgccgcg gaactcgtat
     4981 acgggcgatt gcgtgttagg ccaacgatta gcccggagtg agccagcgtc caactctgcc
     5041 ccaaaacgat tccgattccg gcgcggaatt cgaatcggct tctgcgggtg ccccgcgcct
     5101 cacgcggcgc tgcgtcttgg cctccccggc gagaaggccg ctatagatcc aacccttctt
     5161 gccgcttgcg gggtcggtca cctgaaccca tccgcgctta cgatcgagga ccacaagctc
     5221 gacgcccttg gcgatcacgc cgagcaccgg cga
//
LOCUS       DASUQP010000160         9040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_79659,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000160 DASUQP010000000
VERSION     DASUQP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79659"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFY74_07055"
     CDS             <1..591
                     /locus_tag="VFY74_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5931492.1"
                     /translation="RVALARALVNGPKVLLLDEPLGALDLKLREQMQEELKALQNALG
                     ITFVFVTHDQGEALSMANRVAVFNEGKIVQVGTPASVYERPESRFVADFVGSSNVLPP
                     TFAELHGGVAKWTSLRPEKIAVHPSGSSVPEKHASTDGEVKMVSYQGAVTRFNIETEK
                     MRVNAEVPAGDTRFKPGDSVRLIWPKSAMVTMEEGA"
     gene            639..1544
                     /locus_tag="VFY74_07060"
     CDS             639..1544
                     /locus_tag="VFY74_07060"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5931493.1"
                     /translation="MLGRLSDLFFTRPKLLTFLLLLPPLLWIGFVYVGSLFALLLQSF
                     YSLDDYSGLIVHEFTLSTYAQLLEAANLQIILRTVTMSALVTIACAIIAFPIAYYAAR
                     YARGWTKALFYLAVMMPLWSSYLVKIYAWKLILAKEGIITWLANNLYLSWLIDGVLAL
                     PVIGGPSLSFSYIGTFLVFVYVWIPYMILPIQAALERVPLNYIEASADLGADPGQTFR
                     TVILPLALPGIIAGSIFTFSLTLGDYIVPTIVGNSRLMIGQAVYTLQGTAGNIPLAAA
                     FTVVPMAIMGVYLWMAKRMGAFDVL"
     gene            1534..2352
                     /locus_tag="VFY74_07065"
     CDS             1534..2352
                     /locus_tag="VFY74_07065"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007817540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5931494.1"
                     /translation="MSSSQNRATPLGLRLSAIAGLLFLHLPLALIFLYAFTTEDRSFQ
                     FPPPGYTTKWFQVAWSERPDIWPPLYLSLKVAAIATVLALIFGTLAAAALSRARFFGR
                     ETISLLFILPIALPGIITGISLRSAFDIMGLDFSIWTIVLGHATFCIVVVYNNAIARF
                     RRLSDNLIEASMDLGANSFQTFWYVILPQIGTALLAGGMLAFALSFDEVIVTTFTAGQ
                     QSTLPIWMLSELIRPRQRPVTNVVAVFVIIVTLLPILAAYYLAREGEHIGGSSK"
     gene            2383..3810
                     /locus_tag="VFY74_07070"
     CDS             2383..3810
                     /locus_tag="VFY74_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX5931495.1"
                     /translation="MLDTKMLIGSNFVAGTEAPDAVLNPKTEAKIVDLPDASPAQVDE
                     AVKAAEKAFQSWSRTTPAERSGLLLKLADAIDREAEAFAALEALNTGKPKIRMLQDEM
                     PAASDCFRFFAGAARNMHGAVAGEYMAGHTSMIRRDPIGVIGSIAPWNYPLMMAVWKL
                     APALAGGNTIVIKPSEQTPLTTLKLAKTIAEIFPEGVVNVVTGRGATTGNALITHPKV
                     AMVSLTGSVDTGRKVLLAASPTVKRTHLELGGKAPVIVFDDADLGSVVSGIKVFGYYN
                     AGQDCTAACRIYAGKKIYDNLVADLSSAVKEIKYNLADDDENEMGPLISSTHKARVQS
                     FVERAGKDRHVSITAGGKAPGGKGFFFEPTVVAGAKQDDEIVRKEVFGPVVSVTRFDD
                     VDQAVAWANDSDYGLASSVWTSDIGKGLATAARLQYGCTWVNTHFMLVNEMPHGGLKF
                     SGYGKDLSMYALEDYTVVRHVMVKL"
     gene            complement(3833..4195)
                     /locus_tag="VFY74_07075"
     CDS             complement(3833..4195)
                     /locus_tag="VFY74_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5931496.1"
                     /translation="MSRSTGPVARLRIVIGQNIALGPGKADLMEAVRETGSIAAACRR
                     MGMSYKQAWLLIETMNACFAKPLVEAVKGGKRGGGARLTPLGDDVLVRFRRMQASSSR
                     AIARDLAALKRVMRKAPG"
     gene            complement(4192..7071)
                     /locus_tag="VFY74_07080"
     CDS             complement(4192..7071)
                     /locus_tag="VFY74_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5931497.1"
                     /translation="MYEAESRSHRAKKEPASPAADARPGDALLDDEKKSKKQLLDELV
                     GLRQRIKDLETFEAERKAIATELKGTRQRLQYLLAVSPAIIYTTKASGDYACTFVSEN
                     LRAIMGYTPQQMTTDPKGWPDHLHPDDAPRAFGEMLPLIGRGGGSVEYRFRHHDGHYI
                     WIQDTFKVVYDDAGNPLELVGAWADITERKRAEQAALEANAEIQETKRYLTRLIESSP
                     DGIIATDKEGRVVLFNEGAESLLGYRAADVIGRPTVELYESEDRAKEVLTEMRKRGGT
                     SAGFESVLRAKDGRLIPVLISASVLLDKDGQEAGMVGFATDLRARKREEEELRKAHDE
                     LEKHVEERTTELKAARERLRYLMTVTPAIVYTNQASDFTCTFVSENVSRIMGFSAWEM
                     LEDKDFWTVRLHPEDAKRIFGEMTPLIDKGGGTLEYRFRHRDGHYIWIQDTFRVIPDD
                     QGRPFEIVGSWADISGRKQAETALGERMAVMNDLETLVGASPAIIYTTQVSGDYACTF
                     VSENLDTIMGYAPWEMRDDKKFWVKRLHPEDADRVFREMDGLVKQGHGALEYRFRHRD
                     GHYIWIQDTLTVAHDADGKPKEIVGSWADVTDRKRVEAELQRMAEQVRLRNEFIRQTF
                     GRYLTDEVVDTVLESPKGLQMGGEKRKLTMMMTDLRGFTSLSERLPADRVVTLLNRYL
                     AAMVPIIKQYEGTIDEFIGDAIFVLFGAPVWHEDDAERAVACAVAMQIALDDVNAANR
                     KEDLPEVEMGIGLHTGQVVVGNIGSPERMKYGVVGSHVNLTSRIQACTTGGQILISET
                     TRKELGNTLKIGKQMEVSAKGVERPIVLFEVLGIGGRHKLALAEAVDTLVELPEEIPL
                     RYEVVESNQVGDAPLTGVLTKVSLKGAEARLQNPVPALSNLKIRLIGKDGEPLPGTLY
                     GKVLGPLPRTSTGVSIRFTSIAPEIEAFLRTVTASGGARAQASSL"
     gene            7304..8305
                     /locus_tag="VFY74_07085"
     CDS             7304..8305
                     /locus_tag="VFY74_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5931498.1"
                     /translation="MINLALQGGGSHGAFAWGALDRLLEDERIAVEGIVGTSAGAMNA
                     VVTAYGLTLGGNKGARQALRRFWEAVAEMGARSVMQPSWLDRMRSPGSLDYSPGWILL
                     DTWSRVFSPYQMNPTNYHPLREILSRQIDFAALRQSAKVKLFICASNVLTNRLHVFEG
                     RDMSIEAVLASGCIPSVFQAIEIDGEHYWDGGYMGNPPFFPIIYNCSSADVVLIMVNP
                     IQIKQVPHTAQAILDRINTLSFNSSLMREMRAINFVNHLIDSGFNEGGRLKKILVHCI
                     DAEDEMGNLGVSSKINVCREFLTWLFELGRKRADDFLREHFDRIGKESSTAIERRFL"
     gene            8408..>9040
                     /locus_tag="VFY74_07090"
     CDS             8408..>9040
                     /locus_tag="VFY74_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5931499.1"
                     /translation="MVQSQTTQPEYSLEGVALLKGLSPDTLLSLRDICSWRRYEPGEP
                     IVEYLDRSDDVFFLVSGEARVIVYSLAGKVVSFHDLGPGDMFGELPAIDERPRSASVE
                     ARSSCLVALMKGSAFQDLVQREPIVLRGLLRQLAGTIRRLSMRVYEFSAAIMAVTGRK
                     LYGEMQNIRARDVYPDEEVKAKLLEKTFPKSAEDMTLKLSNVTAAFYGLTL"
BASE COUNT         1549 a         2934 c         2756 g         1801 t
ORIGIN      
        1 cgcgtggcgc ttgcccgcgc gctcgtgaat ggaccgaaag tgctgctgct cgacgagcct
       61 ctcggcgcgc tcgacttgaa gctgagggag cagatgcagg aggagctgaa ggcgttgcag
      121 aacgcgctcg gcatcacctt cgtgtttgtg acccacgacc agggcgaggc gctgtcgatg
      181 gcaaaccgcg tggccgtgtt caacgaaggc aagatcgtcc aggtcggaac gccggcctcc
      241 gtgtatgagc ggccggagtc gcgcttcgtc gccgactttg tcgggtcgtc gaacgtgctt
      301 ccgcccacct tcgccgaact tcatggcggc gttgccaaat ggacaagcct ccggcccgag
      361 aagatcgcgg tgcacccttc cggctcgtcc gtgcctgaga agcacgcctc gaccgacggc
      421 gaggtcaaga tggtcagcta tcagggtgcg gtcacccggt tcaacatcga aacggaaaag
      481 atgagggtca acgccgaagt gcctgccggc gacacgcgct tcaagcccgg cgacagcgtg
      541 cggctcatct ggccgaaatc ggccatggtc accatggagg agggcgcgtg agccaagccg
      601 cggcagtggt gctgacgacg cccggacgcg gagaggggat gctcggccgc ctctccgacc
      661 tcttcttcac gcgcccgaag ctcctgacct tcctcttgtt gctgccgccg cttttgtgga
      721 tcggctttgt ctatgtcggc tcgctcttcg cgctcctgtt gcagagcttc tactcgcttg
      781 acgactactc cgggctcatc gtccacgaat ttaccctttc gacctacgcg cagctgctcg
      841 aggcggccaa tctccagatc atcttgcgca cggtgaccat gtcggcgctg gtcaccatcg
      901 cttgcgcgat catcgccttt ccgatcgcat actatgccgc gcgctacgcc cgtggctgga
      961 ccaaggcgct gttctatctc gcggtgatga tgccgctctg gtcgagctat ctggtcaaga
     1021 tctatgcctg gaagctgatc ctggccaagg aaggcatcat cacctggctc gccaacaacc
     1081 tctatctttc gtggctgatc gacggcgtgc tcgcgctgcc cgtgattggc ggcccgtcgc
     1141 tgtcgttcag ctacatcggc acgttcctcg tcttcgtcta tgtgtggatt ccgtacatga
     1201 tcctgccgat ccaggcggcg ctcgagcggg tgccgctgaa ctatatcgag gcctcggccg
     1261 atctcggcgc ggatccgggg cagacgttca ggacggtgat tcttcccttg gcgcttcccg
     1321 gcatcatcgc cggctcgatc ttcaccttct cgctgacgct cggcgattac atcgttccga
     1381 ccattgtcgg caactcgcgt ctcatgatcg gacaggcggt ctacaccttg cagggcacgg
     1441 ccggaaacat accgcttgcg gccgccttca ccgtcgtccc gatggcgatc atgggcgttt
     1501 atctgtggat ggcgaaacgc atgggggctt tcgatgtcct ctagccagaa ccgcgccacg
     1561 ccgctcggct tgcgcctttc cgccatcgcg gggcttctgt tcctgcatct gccgctcgcg
     1621 ctcatcttcc tctacgcctt caccacggag gatcgaagct tccagttccc gccgccgggc
     1681 tacaccacca aatggtttca ggtggcctgg tcggagcggc ccgacatctg gccgccgctc
     1741 tatctctcgc tgaaagtggc ggcgatcgcg accgtccttg ccttgatctt cggtacgctc
     1801 gccgccgcgg ccttgtcgcg cgcccgcttc ttcggccgcg agacgatctc gctcctgttc
     1861 atcctgccga tcgcgcttcc gggcatcatc accggcatct cgctgcgctc ggccttcgac
     1921 atcatgggtc tcgacttctc gatatggacc atcgtgctcg gtcacgccac gttttgcatc
     1981 gtcgtcgtgt acaataacgc catagcgcga ttccgccggc tttcggacaa cctcatcgag
     2041 gcgtcgatgg atctcggcgc caatagcttc cagaccttct ggtatgtgat cctgccgcag
     2101 atcgggacgg cgctgctcgc gggcggcatg ctcgcctttg cgctttcgtt cgacgaggtc
     2161 atcgtcacga cgttcacggc gggccagcaa tcgaccctgc caatctggat gctgtcggag
     2221 ctgatacgcc cgcgccagcg cccggtgaca aacgtcgtcg ccgtttttgt cattatcgtc
     2281 acgcttctgc cgatccttgc cgcctattat ctggcgcgcg agggcgagca cataggcgga
     2341 tcgtcgaaat agcgcgctta actttcaggt ggaggatcat ccatgctcga cacgaaaatg
     2401 ctgatcggct cgaacttcgt cgcgggcacc gaggctcccg acgccgtgct caaccccaag
     2461 accgaggcga agatcgtcga cttgcccgat gcctccccgg cccaggtcga tgaggcggtg
     2521 aaggccgcgg agaaagcgtt ccagtcctgg tcgcgcacca cgcccgccga gcgctctggt
     2581 cttctcctga aacttgccga cgcgatcgac cgcgaggcgg aagccttcgc cgcgctcgag
     2641 gcgctgaaca cgggcaagcc gaagatacgc atgctgcagg acgagatgcc ggcggcttcc
     2701 gattgcttcc gcttcttcgc gggcgccgcg cgcaacatgc acggggccgt cgccggcgag
     2761 tacatggcgg gccatacgtc catgatccgc cgcgacccga tcggcgtcat cggctcgatc
     2821 gcgccgtgga actatcccct gatgatggcc gtgtggaaac tcgccccggc cctggccggc
     2881 ggcaacacca tcgtcatcaa gccctcggag cagacgccgc tgacgacctt gaagctcgcc
     2941 aagaccatcg ccgagatctt ccccgaaggg gtcgtcaatg tcgtgaccgg ccgcggcgca
     3001 acgaccggaa acgccctcat cacgcacccc aaggtcgcga tggtgtcgct caccggcagc
     3061 gtcgacacgg gaaggaaggt gcttctcgcg gcatctccta cggtgaaacg cacgcatctc
     3121 gagctcggcg gcaaggcgcc ggtcatcgtc ttcgacgatg ccgacctcgg ctcggtcgtc
     3181 tccggcatca aggtgttcgg ctattacaac gcgggccagg actgcacggc ggcgtgccgg
     3241 atctatgccg gcaagaagat ctacgacaat ctggtcgccg acctttcgag cgcggtcaag
     3301 gagatcaagt acaatctggc ggacgacgac gagaacgaga tggggccctt gatctcgtcg
     3361 acgcataagg cccgcgtgca gagtttcgtc gagcgcgccg gcaaagatcg ccacgtctcg
     3421 atcacggcgg gcggcaaggc ccccggcggc aagggcttct ttttcgagcc gaccgtcgtt
     3481 gccggggcga agcaagacga tgagatcgtg cggaaagagg tgttcggtcc ggtcgtgtcg
     3541 gtcacgcgct tcgacgatgt cgaccaggcg gtggcctggg ccaatgacag cgactatggc
     3601 ctcgcctcat cggtgtggac ctccgacatc ggcaaagggc tcgccacggc cgcgcggctc
     3661 caatacggct gcacttgggt caacacgcat ttcatgctgg tgaacgagat gccgcatggc
     3721 gggctgaaat tctccggtta cggcaaggac ctgtcgatgt acgcgctcga ggactacacc
     3781 gtcgtgcgcc atgtcatggt caagctgtaa gctcgccgcc ggttgacccg gcctatcccg
     3841 gcgcttttcg catcacgcgc ttcaaggcgg cgaggtctct cgcaatcgcg cgcgaagatg
     3901 aagcctgcat gcgccggaaa cgaaccagca cgtcgtcgcc cagcggtgtc aggcgggcgc
     3961 cgccgcctcg cttgccgccc ttcacggctt cgaccagcgg cttggcgaag caggcattca
     4021 tggtctcgat caggagccaa gcctgcttgt agctcatgcc catgcgcctg caagcggcgg
     4081 cgatcgatcc cgtctcgcgg acggcctcca tcaagtcggc cttgcccggg cccaaagcga
     4141 tgttctgtcc gataacgatg cggagccgcg ccacggggcc ggtcgaccgg ctcacaggga
     4201 tgaggcttgc gcgcgcgcgc cgccggacgc cgtaacagtt cgcaggaacg cctcgatctc
     4261 gggggcaatc gaggtaaagc ggatcgagac gcccgtgctt gttctcggca agggaccgag
     4321 caccttgccg tagagcgttc cgggcagcgg ttcgccatcc ttgccgatga gccggatctt
     4381 gagattgctc aaggcaggaa cggggttttg cagccgcgct tcggccccct tcaaggagac
     4441 tttcgtcagg acccccgtca acggggcgtc gccgacctgg ttcgattcca cgacctcgta
     4501 gcggagcgga atctcctccg gaagctcgac gagcgtatcg accgcctcgg caagcgcgag
     4561 cttgtgccgc cctccgattc ccagaacctc gaacagaacg atgggacgct cgacaccctt
     4621 ggcgctgacc tccatctgct tgccgatctt gagcgtgttg ccgagctcct tgcgggtcgt
     4681 ctccgagatc aggatctggc cgccggtcgt acaggcttgg atgcgcgagg tgagattgac
     4741 atggcttccg accacaccgt acttcatccg ctcgggcgag ccgatattgc cgacaacgac
     4801 ctggccggta tgaagcccga tgcccatctc cacctcgggc aggtcctctt tccggttcgc
     4861 cgcattgacg tcgtcgagcg caatctgcat ggcgacggcg caggccacgg cccgctcggc
     4921 gtcgtcctcg tgccaaaccg gagcgccgaa caggacgaag atcgcgtccc cgatgaactc
     4981 gtcgatcgtg ccttcgtact gcttgatgat ggggaccatc gcggcgagat agcgattgag
     5041 caacgtcacc acgcgatcgg ccggcaggcg ttccgacagc gacgtgaagc cgcgcagatc
     5101 tgtcatcatc atggtcagct tgcgcttctc cccgcccatt tgcagtccct tgggcgattc
     5161 aagcaccgtg tcgaccacct cgtcggtgag gtagcggccg aaggtctggc gaatgaactc
     5221 gttgcgcagt ctcacttgct cggccatgcg ttgcagttcc gcttcgacgc gcttgcggtc
     5281 ggtgacgtcg gcccaggacc ccacaatttc cttcggcttt ccgtcggcat cgtgcgccac
     5341 cgtaagagta tcctggatcc aaatgtagtg gccgtcgcga tggcggaagc ggtactcgag
     5401 agcgccgtgc ccctgtttga ccagaccatc catttcacga aacacccggt ccgcatcttc
     5461 agggtgaaga cgcttgaccc agaacttctt gtcgtcgcgc atctcccatg gcgcatagcc
     5521 catgatcgta tcaaggttct cgctgacgaa ggtgcaggca tagtccccgg aaacctgcgt
     5581 cgtgtagatg atcgccgggc tcgcgccgac gagcgtctcg aggtcgttca tcaccgccat
     5641 gcgctcaccg agcgcggtct cggcctgctt gcggccggaa atatcggccc acgatccgac
     5701 gatctcgaac gggcgtccct gatcgtcggg gatgaccctg aacgtgtcct ggatccagat
     5761 gtaatgtccg tcccgatgcc tgaagcggta ttccaaagtc ccgccaccct tgtcgatcag
     5821 cggggtcatc tccccgaaga tgcgcttggc gtcctcggga tggaggcgca cggtccagaa
     5881 atccttgtct tcgagcatct cccaggcaga gaatcccatg atgcgagaca cgttctcgct
     5941 gacgaaggtg catgtgaagt cgctcgcctg gttggtgtag acgattgccg gcgtgaccgt
     6001 catgagatag cgcaatcgct cgcgcgccgc cttcagttcc gtggtgcgct cctcgacatg
     6061 cttctccaat tcgtcgtgcg ctttgcgaag ctcctcctcc tcgcgcttgc gcgccctgag
     6121 atcggtggca aacccgacca tgccggcttc ctggccgtcc ttgtcgagga gaacggacgc
     6181 cgagatgagc acgggaatga ggcgcccgtc tttggccctg agcacggact caaacccggc
     6241 ggatgtgccg ccgcgcttgc gcatctccgt caggacctct ttcgccctgt cctcgctctc
     6301 atagagctcg accgtgggac gcccaataac gtcggcggcg cgatagccga gcaatgattc
     6361 ggcgccttcg ttgaagagga cgacgcgacc ctccttatcg gtggcgataa tgccatcggg
     6421 cgaactttca atgagccgcg tcaggtagcg cttggtttct tgaatctcgg cattggcttc
     6481 gagcgcagcc tgctcggcgc gcttgcgctc ggtgatatcg gcccacgccc cgacgagctc
     6541 gagcgggttg cccgcgtcgt cgtagacgac cttgaacgtg tcctggatcc agatgtagtg
     6601 cccgtcgtga tggcggaatc gatattcgac gcttccaccg cctcgcccga tgaggggaag
     6661 catctcgccg aacgcgcgcg gagcatcatc gggatggaga tgatcgggcc agcctttcgg
     6721 atcggtcgtc atctgctggg gcgtgtagcc catgatggcg cgcaaatttt cgctgacgaa
     6781 ggtgcaggcg tagtcgcccg acgccttcgt ggtgtagatg atcgcgggac tgacggcgag
     6841 caggtactgc aggcgctggc gcgtgccttt gagctccgtg gcgatcgcct tgcgttcggc
     6901 ctcgaaggtt tccaggtctt tgatgcgctg gcgcaggccg accagctcgt ccaagagctg
     6961 cttcttgctc ttcttctcgt cgtcaaggag ggcgtcgccg ggccgcgcgt cggccgcagg
     7021 gcttgcgggt tccttcttgg ctctatggga ccggctctca gcttcgtaca tgactgcgcc
     7081 tctccgaggc ggagcgcaac gcgggctagc gcctgcttgg gcgacgtcgc tgcccgggtt
     7141 acttcatttg cagctgcggc tactgtccat ttccgcggcg aagtcttagc atgtcttgcg
     7201 caagagcacg agtatccggc caaaacccat ggcaatagag ggcgcggact tcccccaggg
     7261 gggcaaaacg gacccccaga cccggcaaac ggccggccac aaagtgatta acctggccct
     7321 tcagggtggg gggtcgcacg gcgcttttgc ctggggggcg ctcgaccggc tgctcgaaga
     7381 cgagcgcatc gcggtcgagg gtatcgtcgg caccagcgct ggcgcgatga atgccgtcgt
     7441 gaccgcctat ggcttgacgc tcggcggcaa taagggggcg cggcaggcgc tccgccgttt
     7501 ctgggaggcc gtcgccgaaa tgggcgcccg ctccgtcatg cagccgtcat ggctcgatcg
     7561 gatgaggagt cctggcagcc tcgattactc gcccggctgg attctcctgg acacgtggtc
     7621 gcgggtgttc tcgccgtacc aaatgaaccc gaccaactac cacccgttgc gcgagatcct
     7681 gagccggcaa atcgatttcg ccgcactccg ccaatccgcc aaggtcaagc tctttatttg
     7741 cgcgagcaac gtcctgacca accgcctgca cgtcttcgaa ggtcgcgaca tgagcatcga
     7801 agctgtgctc gcctcgggct gcatcccgtc ggtgttccag gcaatcgaga tcgacggcga
     7861 gcattattgg gacggcggct acatgggcaa cccgcccttc ttcccgatca tctacaattg
     7921 cagctccgcc gatgtggtgc tcatcatggt caaccccatc caaatcaaac aggtgccgca
     7981 caccgcgcaa gccatcctcg accgcatcaa tacgctgagc ttcaactcaa gcctcatgcg
     8041 cgagatgcgc gccatcaact tcgtcaacca cctgatcgac agcggcttca acgagggcgg
     8101 ccggctcaag aaaattcttg tccactgcat cgatgccgag gacgagatgg ggaatctcgg
     8161 cgtgtcgagc aagatcaatg tctgccggga attcctgaca tggctgttcg agctgggccg
     8221 caaacgcgcc gacgatttcc tccgcgagca tttcgacagg atcggcaagg aatcctcgac
     8281 ggcgatcgag cggcgttttc tctagttttc ggccccggcg atgcattccg gcgttgcatg
     8341 ttttgaatgc aaccggcgtg ccgctcggct atgatccgcc cccgcgaacg gagcgtcggt
     8401 tgcggcgatg gtgcagtcac agaccacgca gcctgaatat tcgcttgagg gagtcgccct
     8461 tctcaagggc ctctccccgg atactttact ttctctccgc gacatttgta gctggcggcg
     8521 ctacgagccg ggcgagccga tcgtcgaata tctcgatcgt tccgacgacg tgttctttct
     8581 cgtgtcggga gaggcgcgcg tcatcgtcta ttctcttgcc ggcaaggtcg tgagtttcca
     8641 cgacttggga cccggcgaca tgttcggcga gctgcccgcc atcgacgagc gtccccgctc
     8701 ggcgagcgtc gaggcccgca gcagctgcct tgtcgccttg atgaagggtt ccgcgtttca
     8761 ggacttggtg caacgggaac caatcgtgct ccggggcctg ttgcgacagc tcgccggcac
     8821 catccgccgg ctgtcgatgc gcgtctatga gttcagcgcg gcgatcatgg ccgtgaccgg
     8881 gcgcaagctc tatggcgaga tgcagaacat ccgcgctcga gacgtctatc ccgacgaaga
     8941 ggtgaaagcc aagctgctgg agaaaacctt tcccaaaagc gcggaagaca tgacgctcaa
     9001 gctctcaaac gtcaccgccg ctttttacgg actgacgctc
//
LOCUS       DASUQP010000161        12809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_352606,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000161 DASUQP010000000
VERSION     DASUQP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352606"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..895
                     /locus_tag="VFY74_07095"
     CDS             71..895
                     /locus_tag="VFY74_07095"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX5931500.1"
                     /translation="MTAIEPGKRPLPAPSLEEDYALLKKAVRAAGDLALTYFRKSIIV
                     KRKVDGSEVSEADLAIDVALKIELHVPRPDYGWLSEETEDDSERLKSSRVWMVDPIDG
                     TNAFLRHVPEWTVSAALVENGRPVLGAVFNPATNEFFHAMRGKGAFLNDKPITASDKS
                     TLGGALLIASGGLFKKKIWKEPWPEVKTKWVNSVAYRLALVACGRADATISLSNKSEW
                     DLAAAALVVEEAGGLVTDHHGEAHRYNRESPRFPSLVASGKALHPLLIERTDRVDL"
     gene            918..1181
                     /locus_tag="VFY74_07100"
     CDS             918..1181
                     /locus_tag="VFY74_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX5931501.1"
                     /translation="MAAKDAKRSGRQLLHLVFGGELTSLEGMEFSNLRALDIVGIYPN
                     YAQAYDAWKEAAQRTVDDAHMRYFIVHLHRLLDPGQEPGGETK"
     gene            1318..3150
                     /locus_tag="VFY74_07105"
     CDS             1318..3150
                     /locus_tag="VFY74_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5931502.1"
                     /translation="MITRSADELQVNYTTKDIVIRIWREHLRPRLGLLVLASLAMLLT
                     AATTGAIPFLIQKIADDVFVAKNAQMVYWITAAIVIVTVVKAASEYIADVTVAYLGHR
                     FVADLRLQMFAKLSRADLNWIQRVHSGRLLSGFLNDATLIRATASRSLVTLGESYLKV
                     IILVGGMFYMDPRFSLLILIFMPVAWFVLSRQRRKMRKSTTQSLQETGDLSALITQTL
                     RGMRIVRAYRQEDREEARAASAINRALEFTMRGTRARAFSSPSIELLTGLGFALAIYF
                     AGTKGVRGDLTLGHFMGFTTAALLIYAPLKSVATLQTQLQEGVAAASRVFGIIDREIG
                     LKEDPDAKALDLERGKIEFRNVSFAYDSETSVLKGVSLTVPPGRTVALVGPSGSGKST
                     LVNLTLRFFDPERGKVLIDGQDIKQLTIESLRDAIALVTQDPVLFDDTIRANIAYGAK
                     PAVESEVIEAAKAAAAHDFIMALPKGYDTRVGEAGGLLSGGERQRIAIARALYKDAPI
                     LLLDEPTSSLDSEAEAKVQSALEELMRGRTVLMIAHRLSTVKKADLICVLDQGRIVET
                     GRHEELVAKGGLYTRLHRTQFGIAGGYTAPQASPDEQVVAVAGK"
     gene            3279..4595
                     /locus_tag="VFY74_07110"
     CDS             3279..4595
                     /locus_tag="VFY74_07110"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX5931503.1"
                     /translation="MTTHDAAKRGLWLYAYRALTRLATPFTPIILAWRTRRGKEERDR
                     RPERYGLASTSRPAGFLAWFNAASVGEANAALPVIEAIAAERPEIRLLLTTATVTSAR
                     LARTRMPKGVLHQYVPLDNQVFVQRFLRHWRPDLAVLVESEIWPNLVLETKALHIPLL
                     LINGRMSASSFKRWRRRPGLSRPLFSAFDLVLAQNDSLAERFSELGAVRALDVGNLKA
                     DAPPPPADLPGRRKLHAALDGRTVWLAASTHQGEDDTVMAAHLKIKSARPDLLTIIVP
                     RHPERGPLIAEQLRAKGLNFALRSEGKLPDACTDVYVADTIGELGLFYALVPVAFVGG
                     SLVDRGGQNPLEPIKLGAAVLTGPKWQNFRDSYDELIRAGGCKQVTDAASLAEAVLHL
                     IEDEDARRTMTERAVGAIAAMGGALPRTLAELENYLPPKTTFKHAS"
     gene            4585..5649
                     /gene="lpxK"
                     /locus_tag="VFY74_07115"
     CDS             4585..5649
                     /gene="lpxK"
                     /locus_tag="VFY74_07115"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX5931504.1"
                     /translation="MPLEAPFWWYRKKGALAFALVPLGRLYGRMAEARYAGVEPYRSA
                     LPVICIGNFTAGGGGKTPTAIAIASLLKALGAKPAFLTRGYGGASEGPVLVAKGQSAE
                     EVGDEPLLLAEAAPTIVSADRAAGAKAIEATGADVIVMDDGFQNPGLAKDLSLVVVDA
                     AAGIGNGLVMPAGPLRAPLAAQTERAGALVVIGDGGKALLLIEAFRQQGKPVLKARMV
                     PRQDRRWLSVLPVIGFAGIARPSKFFDTLRSNGARLIDARSYPDHYRYSERQARSLLR
                     EAKDFNAMLVTTEKDWVRLPEGDGSDAAELKHRSRPFAIAIEFDDVAAVKALLSTAMA
                     KARAEGHQPIKARGGRGVSN"
     gene            complement(5651..5890)
                     /locus_tag="VFY74_07120"
     CDS             complement(5651..5890)
                     /locus_tag="VFY74_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEX5931505.1"
                     /translation="MNRFERNTTFKSEAKLKYLDGEVQVVTPGEFVRCAVTGRTIPLD
                     DLRYWSVELQEAYADAASSLKRYRETRTQPGEAAE"
     gene            complement(5964..7415)
                     /gene="xseA"
                     /locus_tag="VFY74_07125"
     CDS             complement(5964..7415)
                     /gene="xseA"
                     /locus_tag="VFY74_07125"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5931506.1"
                     /translation="MNETMVDPTTAAAPATNLVEYSVSELAFALRKTVEEAFGLVRLR
                     GEISGFKGRHTSGHCYFTLKDDKAAIEAVIWRSTFQRLKFKPEAGLEVIVKGRVTTYP
                     DRSRYQIVIDELEPAGIGALMALLEQRKRMLAAEGLFEAARKKTLPFLPRVIGVVTSP
                     TGAVIRDILHRLADRFPCHVLVWPVRVQGETCALEVSAAIIGFNAIEVGRPIPRPDLI
                     IVARGGGGFEDLWGFNEESVARAVAASAIPLISAVGHETDWTIIDLVADFRAPTPSAA
                     AERAVPVRSDLLIAVTQHGLRVATSLRRRIAVERNRFNGLARLLPRKANILDLPRQRF
                     DTASGRLKRALIANARVHRNWFQRSVGRLNAHVLVRAARSGRDRLVRLDRARSRAGQA
                     LLARSRSRLDGQAKLLETLSYQNVLARGFALVRTADGIMIRRAAQVKSGAALDIEFAD
                     ARIGAHADASVRKVKDEAKAPPRRRDEKQGRLL"
     gene            7517..8827
                     /gene="purD"
                     /locus_tag="VFY74_07130"
     CDS             7517..8827
                     /gene="purD"
                     /locus_tag="VFY74_07130"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX5931507.1"
                     /translation="MDVLLIGGGGREHALAWKLRQSPLLDRLYCAPGNAGIEEVAACV
                     ALDIADHAAVIRFCKDNAIGLVVIGPEAPLVAGLSDDLEASGIKVFGPSRTAARLEGS
                     KGFTKDLSAAYGIPTAAYRRFADAASAKAYAAEQTLPIVVKADGLAAGKGVVIAETHA
                     DAAAAIDACFAGAFGPAGAEVVIEEFLEGEEASFFALTDGGTVLPLATAQDHKRAFDG
                     DQGPNTGGMGAYSPAPIMTPELCASVMDQIIAPTVRAMRERGTPFKGVLYAGLMIKDG
                     APKLIEYNVRFGDPEAQALMLRLKSDLLPALLAVAEGRLADVALDWHDDAALCVVMAA
                     NGYPGAYAKGSEIRGLEKAGADPNVQIFHAGTRREGDRILADGGRVLGVTARGNTIAE
                     AKARAYAAIDKIDWPGGFYRRDIGRRAVAGLEGRIGLTNPQDIN"
     gene            8936..9256
                     /locus_tag="VFY74_07135"
     CDS             8936..9256
                     /locus_tag="VFY74_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HEX5931508.1"
                     /translation="MLARTEQPDPSAELLGFVSSRSFTTVLADPPWQFTNRTGKVAPE
                     HRRLSRYPTLTLDEIAGLPVSKITTSTAHLYLWIPNALLPEGLAVMRAGLHLQVQHCL
                     AQGA"
     gene            9249..9593
                     /locus_tag="VFY74_07140"
     CDS             9249..9593
                     /locus_tag="VFY74_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HEX5931509.1"
                     /translation="MRKDGGSDGRGVGFYFRNVTEMVLFGVRGKNARTLAPGRRQVNL
                     VATRKREHSRKPDELYPLIEACSPGPYLELFARGARENWVAWGNQANDAYKPSWKTYT
                     YNSSANHLGCTE"
     gene            complement(9710..10525)
                     /locus_tag="VFY74_07145"
     CDS             complement(9710..10525)
                     /locus_tag="VFY74_07145"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HEX5931510.1"
                     /translation="MLTYPRMLAAGELDPRGPKLQIATPRLGSEPLFDKLRDRGFQVQ
                     TLSHAKAILQGDFPSAAVELEEVLLNAQVPIEEIIAGGGGEAKGTQRLRKGLQGRGWA
                     TKTFTIVKTINGEPREAISHKMDHVKSFANPNEGGKEYTLALEIEWNNKDPFFDRDLE
                     NFKRLHADGAISVGIIVTRGRSLHASMREHVTRFVEKRKIRSLDDLTRWGYNPTIRQR
                     NAIVKRFERANNPLSFSQSFVAQFVSDKFGEATTHWRKLEDRVNRGEIRVHSS"
     gene            complement(10643..11128)
                     /locus_tag="VFY74_07150"
     CDS             complement(10643..11128)
                     /locus_tag="VFY74_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX5931511.1"
                     /translation="MWYRGWMIGALLTVAVAASALAKDMTPRQVADAYMAAWNAHDVD
                     AAAALLADDVTYFDATVGHPEKGRDAARDKVIKLFVTAAPDLTWKMTGEPIESANGIA
                     YEWTFAGNNTGAWGPDTPATGKPFSFNGVTFIRVENGKIVYQGDYYDGLGFQKQLGWI
                     Q"
     gene            11453..11800
                     /locus_tag="VFY74_07155"
     CDS             11453..11800
                     /locus_tag="VFY74_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931512.1"
                     /translation="MALALAGTAGLVLGLGLAAGSDRAHAVRLVTAGPPSHASIIEPV
                     RIYRRGRTPIYPYYYNPGRPGGYSFYFGFVPYAPGDIENQALQRQFYPHSTEWPPGLY
                     GGPQPGWGPKRWQ"
     gene            complement(11815..12246)
                     /locus_tag="VFY74_07160"
     CDS             complement(11815..12246)
                     /locus_tag="VFY74_07160"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX5931513.1"
                     /translation="MDLALQEAERARDRGEVPIGAVLVSAGGEVLARAGNHTLALHDP
                     TAHAELLAIRAACAKLGNERLDGCDLYVTLEPCAMCAAAISFARIRRLYFGAADPKGG
                     AVEHGPRIFTQPTCHHAPEVVGGLGETQAASLLKAFFASRR"
     gene            12392..12809
                     /locus_tag="VFY74_07165"
                     /pseudo
     CDS             12392..12809
                     /locus_tag="VFY74_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
BASE COUNT         2262 a         4139 c         4087 g         2321 t
ORIGIN      
        1 gacctttacc tgctcacatg gtttagagaa cttttctgaa aagtttcgag aatcttccgc
       61 aaactgcaca atgaccgcta tcgagccagg caaacggccg cttcccgccc cgtccctcga
      121 agaagattac gcccttctta agaaagccgt gcgcgctgcg ggcgaccttg cgctcaccta
      181 tttccgcaaa tcgatcatcg tcaaacggaa ggtcgacggc agcgaggtga gcgaagccga
      241 cctcgcgatc gacgtcgcgc tcaagatcga gcttcacgtg ccgcgccccg actatggctg
      301 gctgtcggag gagacggagg acgattcgga gcggctcaag agcagccggg tgtggatggt
      361 cgatccgatc gacggcacca acgctttcct gcgtcacgtg ccggaatgga cggtctcggc
      421 cgcgctggtc gagaacgggc ggccggtgct cggtgcggtg ttcaatccgg ccaccaacga
      481 gtttttccac gccatgcgcg ggaaaggcgc cttcctcaac gacaagccga tcacggcaag
      541 cgataaaagc accctcggag gagcgctgct catcgcgagc ggcggtctct tcaagaaaaa
      601 gatctggaag gagccgtggc ccgaagtgaa gaccaaatgg gtgaactcgg tagcctatcg
      661 gctcgctctc gtcgcatgcg gcagggccga cgccaccata tcgctctcca ataagagcga
      721 gtgggatctc gccgccgccg cgcttgtggt ggaagaggcg ggcggccttg tcaccgatca
      781 tcacggcgag gcgcaccgct ataaccgcga gtccccgcgc ttcccgagcc tggtggcgag
      841 cggcaaggcg ctccatccgt tgttgatcga gcgtaccgac cgcgtcgatc tctgagacgc
      901 gtggaggaaa gggcagcatg gctgcaaagg acgcgaagag gagcggccgg cagttgctgc
      961 acctcgtgtt cggcggcgaa ctgacctcgc tcgagggaat ggagttcagc aatctcaggg
     1021 cactcgatat cgtcggaatt tatcccaatt acgcgcaagc ctatgacgcg tggaaggagg
     1081 cggcgcagcg gaccgtggac gacgcccata tgcggtattt tatcgtccac ctgcatcgcc
     1141 tgctcgatcc tgggcaggag ccgggcggag agaccaagta gccgcttcgt gcctggcctt
     1201 tttgccttcg aggatccccc cgccgcatgc tagaaggcgg gaaggcgggc gcaaggggca
     1261 cgggcgcgcc gagaaacggg gcatagtttg aaagatcagc tgttcaggga cgcaagcgtg
     1321 atcacgcgga gcgccgacga gctccaggtc aactatacga ccaaggacat cgtgatccgc
     1381 atctggcgcg aacatttgcg gcctcggctg ggcctcctcg tgctcgcttc gctcgccatg
     1441 cttctgaccg ccgccacgac cggggccatc ccgttcctca ttcaaaagat cgccgacgac
     1501 gtgttcgtcg ccaagaacgc gcagatggtc tactggatca ccgcggcgat cgtcatcgtg
     1561 actgtcgtca aggccgcatc ggagtatatc gccgacgtca ccgtcgccta tctcggccat
     1621 cgcttcgtcg ccgatctgcg cctgcagatg ttcgccaagc tttcgcgcgc cgatctgaac
     1681 tggatccaga gggtgcattc cgggcgattg ctctccggct ttctcaacga cgcaacgctc
     1741 atccgggcga cggcgagccg ctctctcgtc actcttggcg agagctacct caaggtcatc
     1801 attcttgtcg gcgggatgtt ttacatggac ccgcgcttct cgctcctgat cttgatcttc
     1861 atgccggtcg cctggttcgt gctctcgcgg cagcggcgca agatgcggaa atcgaccacc
     1921 cagtcgttgc aggaaaccgg cgacctttcg gcgctcatca cccagacctt gcgcggcatg
     1981 cgcatcgtgc gcgcctatcg ccaggaggat cgcgaggaag cgcgcgcggc ttccgccatc
     2041 aatcgggcgc tcgaattcac tatgcgcggc acgcgcgcgc gcgcgttctc cagcccctcc
     2101 atcgagctcc tcaccggatt gggctttgcg ctcgcgatct acttcgccgg aaccaagggg
     2161 gtgcgcggcg accttaccct cggccatttc atgggtttca cgacggcggc gcttctcatc
     2221 tacgcgccgc tcaagagcgt cgccaccttg cagacgcagc tccaggaagg cgttgccgcc
     2281 gcaagccggg tgttcggcat catcgaccgc gagattggcc tgaaggagga ccctgacgcc
     2341 aaggcgcttg acctcgagcg cggcaagatc gagttccgca acgtctcctt cgcctacgat
     2401 tccgagacga gcgtcttgaa gggggtcagt ctcaccgtgc cgcccgggcg cacggtggcg
     2461 ctggtcgggc cctccggctc gggcaagagc acgctcgtca atctcacctt gcgtttcttc
     2521 gatcccgagc gcgggaaggt gctgatcgac gggcaggaca tcaagcagct caccattgag
     2581 agcctgcgcg atgccatcgc gctcgtgacc caggacccgg tgctgttcga cgataccatc
     2641 cgcgccaata tcgcctatgg agcgaagcct gcggttgaga gcgaggtgat cgaagcggca
     2701 aaggcagcgg cggcgcatga tttcatcatg gcgctgccca agggctacga cacccgcgtc
     2761 ggcgaggccg gcgggctgtt gtcgggcggc gagcgccagc gcattgccat cgcgcgcgcg
     2821 ctttacaagg atgcgccgat ccttctcctc gacgagccca cgagttcgct cgactcggag
     2881 gccgaggcga aggtgcagtc cgcgctcgaa gagctgatgc gtggccgtac ggtgctcatg
     2941 atcgctcacc gcctctcgac ggtgaagaag gccgatctca tctgcgtgct cgaccagggg
     3001 cggatcgtcg agaccggtcg ccacgaagag ctggtcgcca aaggcggcct ctacacgcgg
     3061 cttcatcgca cgcaattcgg cattgccgga ggctacacgg cgccgcaggc aagcccggac
     3121 gaacaagtcg tcgccgtggc cggcaagtag cgaccgagaa gatgcggtat gttggaacgc
     3181 ctggcagcaa ggcgattgaa aaagggcggg ttgcaatcga cagtgctctc gccgaggcgc
     3241 ccgcgcgcgc atatgctctc gccggagctc gtgatccatt gaccacgcat gacgccgcca
     3301 agcgagggtt gtggctctac gcctaccggg cgctgacgcg cctcgcgacc ccgttcacgc
     3361 cgatcatcct cgcctggcgc acgcgccgcg gcaaagagga gcgcgaccgg cgccccgagc
     3421 gctatgggct tgcaagcaca tcccggcccg cgggcttcct tgcctggttc aatgccgcga
     3481 gcgtcggcga ggccaatgcc gcgctgccgg tgatcgaggc catcgccgcc gagcggccgg
     3541 aaatccgcct tctgctcacg accgccacgg tgacatcagc gcggcttgcg cgaacgcgga
     3601 tgccgaaggg cgtgctgcat caatatgtgc cgctcgacaa tcaggttttc gtgcagcggt
     3661 tcttgcgcca ttggcgtccc gatcttgccg tgctggtcga gtcagagatc tggccgaacc
     3721 tcgtgctcga gaccaaggcg cttcatattc cgctcttgct catcaacggc cgcatgtcgg
     3781 cctcgagctt caagcgctgg cggcggcggc cgggcctgag ccggccgctg ttctcggcct
     3841 tcgatctcgt gctggcgcag aacgacagtc tcgccgagcg tttctcggag ctcggcgcgg
     3901 tccgcgcgct cgatgtcggc aatctcaagg ccgatgcgcc gccgccgccc gccgatcttc
     3961 ccggccggcg caagctccat gccgcgcttg acggacgcac cgtgtggctc gccgcgagca
     4021 cccatcaagg cgaggatgac accgtcatgg cggcgcatct caagatcaag tccgcgcggc
     4081 ccgatctcct caccatcatc gtgccgcgcc atcccgagcg cgggccgctc atcgccgaac
     4141 agcttcgcgc caagggcctc aactttgcgc tacgctccga aggaaagctg ccggacgcct
     4201 gtacggacgt ttacgtggcc gacacgatcg gcgagcttgg cctgttctat gcgctcgtcc
     4261 ccgtcgcctt tgtcggcggc tcgctggtcg atcgcggcgg acagaatccg ctcgagccga
     4321 tcaagctcgg cgccgccgtt ctcaccggtc cgaaatggca gaatttccgc gactcctacg
     4381 acgagctcat ccgcgcgggc ggctgcaaac aggtgaccga cgcggcaagc cttgccgagg
     4441 ccgtgcttca tctcatcgaa gacgaagacg cgcgccgcac catgaccgag cgtgctgtcg
     4501 gcgccattgc ggccatgggc ggggcgctcc cgcgcacgtt ggccgagctc gagaactatc
     4561 tcccgccaaa gaccaccttc aaacatgcct cttgaagcgc cgttctggtg gtatcgcaag
     4621 aagggggcgc tcgcctttgc gctcgtgccc ttgggccggc tctatggccg catggccgag
     4681 gcgcgctacg cgggggtcga gccgtaccgc tccgcccttc ccgtgatctg tatcggcaat
     4741 ttcacggccg gcggcggcgg caagaccccg accgcgatcg caattgcgtc tctgctcaag
     4801 gcgctcggcg cgaagcccgc tttcctcacc cgcgggtatg gcggggcgag cgaagggccg
     4861 gtgctcgtcg ccaaggggca aagcgccgag gaggtggggg atgagccgct cctgctcgcc
     4921 gaggcggcgc caaccatcgt ctcggccgat cgggccgccg gcgccaaggc catcgaggcg
     4981 accggcgccg atgtgatcgt gatggatgac gggttccaaa acccgggcct tgccaaggat
     5041 ctatccctcg tggtggtgga tgccgcggcc ggtatcggca atggtctcgt catgccggcg
     5101 ggaccgctca gggcgccgct tgcggcgcaa acggagagag cgggtgcgct cgtcgtcatc
     5161 ggcgatggcg gcaaggctct gctcctgatc gaggccttca ggcagcaggg caagccggtg
     5221 ctgaaagcgc gcatggtgcc gcgccaggac cgacgctggc tcagcgtgct gccggtgatc
     5281 ggttttgccg gaatcgcgcg gccatcgaaa ttcttcgaca cgcttcgctc aaacggcgcc
     5341 cggctgatcg atgcgcgttc ctaccccgac cattaccgct atagcgagcg gcaggcccgc
     5401 agcctgctca gagaggcaaa ggacttcaac gccatgctcg tcaccaccga gaaggactgg
     5461 gtaaggcttc ccgagggcga cggcagcgat gcggccgagc tcaagcaccg ctctcgcccc
     5521 ttcgccattg cgatcgagtt cgacgatgtg gcggcggtga aggctctgct ttcgacggcg
     5581 atggcgaagg cgcgggccga agggcaccaa cccatcaagg cgaggggcgg caggggcgtg
     5641 tcaaactgag ttactcggcc gcttcccccg gttgcgtccg cgtctcgcga tagcgcttga
     5701 gggacgaggc ggcgtcggca taggcctctt gcagttcgac gctccaatag cgcagatcat
     5761 cgagcggaat ggtgcggccc gtcaccgcgc agcgcacgaa ctcgccaggc gtcaccacct
     5821 gcacctcgcc gtcgagatat ttgagcttgg cctcgctctt gaaggtggta ttgcgttcga
     5881 aacgattcat cgaggtcgag tggccctttg cttctgccgt cgagcgtcac catacaatca
     5941 agcggtgcga gaaggccagc gctctaaagc agtcttccct gcttttcgtc gcggcggcgc
     6001 ggcggcgctt tcgcttcgtc tttcaccttg cgcacgctcg catccgcatg agcgccaatg
     6061 cgggcatcgg caaactcgat gtcgagagcc gcgcccgatt tcacttgggc ggcacggcgg
     6121 atcataatgc cgtcggccgt gcgcaccagc gcgaagccgc gcgcgagcac attctggtag
     6181 ctcagcgtct cgagcagctt cgcctggccg tcgagccgcg agcgcgagcg cgcgagcaag
     6241 gcttgccccg ccctcgaccg cgcgcggtcg aggcggacga gccggtcgcg gccgcttcgc
     6301 gcggcacgaa ccagaacatg ggcgttcagc cggccgacgc tccgctggaa ccaattgcga
     6361 tgcacgcgcg cgttggcaat gagcgcgcgc ttgagacgcc cgctcgccgt atcgaaacgc
     6421 tggcgcggca gatcgagaat attggccttg cgcggaagca agcgcgccag cccgttgaac
     6481 cgattgcgct cgacggcgat ccggcgcctg agcgaggtcg cgacgcgaag gccgtgctga
     6541 gtcacggcga tgaggaggtc ggagcgcacc ggcacggcgc gctcggcggc ggcggacggc
     6601 gtcggcgcgc ggaaatcggc gacgaggtcg atgatggtcc agtcggtctc gtgccccacc
     6661 gccgagatca gcgggatcgc gctcgcggca accgcccgcg ccacgctttc ctcgttgaag
     6721 ccccacagat cctcgaagcc gccgccgccg cgtgccacga tgatgaggtc ggggcgcgga
     6781 atgggacgac ccacttcgat cgcgttgaag ccgatgatgg cggcggacac ttcgagcgcg
     6841 caggtctcgc cctgcactct caccggccag accaacacgt ggcagggaaa gcggtccgca
     6901 agccgatgaa ggatgtcgcg gatcaccgcg cccgtcggcg aggtcacgac gccgatgact
     6961 cgcgggagga aaggcagcgt cttcttgcgc gccgcttcga acaggccctc ggcggcgagc
     7021 attctcttgc gctgttcgag cagcgccatg agcgcgccga taccggcagg ctcgagctcg
     7081 tcgatgacga tctgatatcg cgaccggtcg ggataggtgg tgacgcggcc tttgacgatg
     7141 acttcgagcc ccgcctctgg cttgaacttg agccgctgga aggtgctccg ccagatcacc
     7201 gcctcgatcg cggccttgtc gtccttcagt gtgaagtagc aatggccgct cgtgtgccgg
     7261 cccttgaagc cggaaatctc gccgcgcaag cgcacgagcc caaacgcctc ctcgaccgtc
     7321 ttgcgaagcg caaacgcaag ctcggagacg ctatattcga cgagattcgt tgctggcgcc
     7381 gcggccgtgg tcgggtcgac catcgtttcg ttcactcggt ttcgcttagc ctgctcttgc
     7441 atgctaagga gcatcaattt tgggggtgtg agcattactc acctcggcgc ctccctacaa
     7501 gcggagcgtg atttcggtgg atgtgctgtt gatcgggggc ggcggccgcg agcatgcgct
     7561 tgcctggaag ctcaggcaaa gcccgctgct cgaccgtctc tattgcgcgc cagggaacgc
     7621 cgggatcgag gaggtcgccg catgcgtcgc gctggacatc gccgatcacg cggcggtcat
     7681 ccgcttctgc aaggacaacg cgatcgggct cgtggtgatc gggcccgagg cgccgctcgt
     7741 cgccgggctc agcgacgatc tcgaagcctc ggggatcaag gtgttcgggc cgtcgcggac
     7801 tgcggcccgg ctcgaaggct cgaaaggctt caccaaggat ctttcagccg catacggcat
     7861 tcccacggcc gcctatcgcc gcttcgccga cgccgcttcc gccaaggcct atgctgcgga
     7921 acagaccttg ccgatcgtgg tcaaggccga tgggctcgcc gccggcaagg gcgtggtcat
     7981 cgccgaaacg catgcggacg ccgcggccgc aattgacgcc tgtttcgccg gcgcgttcgg
     8041 gccggccggc gccgaggtgg tgatcgagga atttctcgaa ggcgaagaag cgagcttctt
     8101 cgctcttact gatggcggga ccgtgctgcc gcttgccacc gcgcaagacc acaagcgcgc
     8161 cttcgacggc gaccagggcc caaacacggg cggcatgggc gcctattctc cggcgccgat
     8221 catgacgccc gagctctgcg cgtccgtcat ggaccagatc atcgcgccga cggttcgcgc
     8281 gatgcgcgag cgcggcacgc cgttcaaggg cgtgctttat gccgggctga tgatcaagga
     8341 cggggcgccg aaactgatcg agtacaatgt gcgcttcggc gatcccgagg cgcaggcact
     8401 gatgcttagg ctcaagtcgg atctgctccc tgccctgctc gccgtggccg aggggcggct
     8461 cgccgatgtc gcgctcgact ggcacgacga cgcggcactc tgcgtggtga tggcggcaaa
     8521 cggctatccc ggcgcttacg cgaagggttc ggagatcagg ggcctcgaga aagcgggcgc
     8581 cgacccgaac gtgcagatct tccacgccgg aaccaggcgc gagggcgacc gcatcctggc
     8641 tgacggcgga cgcgtgctcg gcgtgaccgc gcgggggaac acgatcgcag aggcgaaagc
     8701 ccgcgcctat gccgcaatcg acaagatcga ctggccgggc ggcttttacc gccgcgacat
     8761 cggacgccgc gccgtggcgg gtcttgaggg ccggataggc ttaacaaatc cacaagatat
     8821 caactgatag aacttgccgg actgtgaaga atgttctatt tttgttccct tattcggcaa
     8881 gctggttcac agcggggaat caccgcattc tccagttccg caccaaggtt cgttgatgct
     8941 ggctagaact gaacagcccg acccgtctgc tgagcttctt ggtttcgtta gctctcgaag
     9001 cttcacaacg gtcctcgccg atccaccctg gcagttcacc aatcgcaccg gaaaggtggc
     9061 gccggagcat cgccggctgt cacggtatcc cacactcaca ttggatgaga tcgccgggct
     9121 tccggtgtca aagatcacaa cctcgacagc gcatctttat ctctggattc ccaatgccct
     9181 tttaccagag gggctggcgg tgatgcgcgc ggggcttcac ctacaagtcc aacattgtct
     9241 ggcacaaggt gcgtaaggac ggcggctcag atggccgtgg cgtggggttc tattttcgca
     9301 atgtcacaga gatggttctc ttcggcgtgc gcggaaagaa tgcccgaaca ctcgccccag
     9361 gacgtcggca ggtcaatctg gtggcgactc gcaagcgcga gcattcgcgc aagcctgacg
     9421 aactttatcc actcatcgag gcctgctcac cgggacctta tttagagctc ttcgccagag
     9481 gtgcgcgcga gaattgggtc gcgtggggta atcaggcgaa cgatgcctac aagccttcat
     9541 ggaaaactta cacctacaac tcttctgcca accatctcgg ctgcaccgaa taaggaacgc
     9601 tattctgttt cgggctcgcc tgcactttcg tctttatgcg cagaggcgat aatttctctc
     9661 accgttgtct cgtcctcgtc aaaggacaca atcgagtccg gcaatccgat caggacgagt
     9721 ggacacggat ttcaccgcga ttgacgcgat cttctagttt ccgccagtgg gtggtcgctt
     9781 caccgaattt gtcgctcacg aattgagcaa caaatgattg gctaaaggag agtgggttgt
     9841 tcgcgcgctc gaatcgcttg acgattgcgt tgcgctgccg gatggtcgga ttgtagcccc
     9901 atcgcgtcag gtcatcgagg cttctaatct ttcgcttctc gacgaaacga gtgacgtgtt
     9961 ctcgcatgct cgcgtgcagg gagcggccgc gcgtcacgat aatgccaacc gagatcgcgc
    10021 catctgcgtg aagccgctta aagttttcca gatcccggtc gaaaaatgga tccttgttgt
    10081 tccattcgat ctcgagcgca agcgtgtatt cttttccgcc ttcattgggg ttggcgaacg
    10141 acttaacatg atccattttg tgtgaaatgg cttcccgggg ttctccgttt attgtcttga
    10201 cgattgtgaa cgtcttggtc gcccagcctc gaccttgaag ccctttgcga agacgttgtg
    10261 tccccttcgc ttcgcctcca ccgccggcga taatctcctc gattggaacc tgcgcattga
    10321 gcaagacctc ctcaagctca accgcagccg acgggaagtc accctgcaag atcgccttgg
    10381 cgtgagacag cgtctgaact tggaagcccc ggtctcgtaa tttgtcaaac aacggctcgc
    10441 tcccaaggcg tggcgtagca atttgcaact tcggcccacg tgggtcgagc tctcctgccg
    10501 ccagcatcct tggataggtg agcatgcgcg gcatcgcagc gtattggtcg cggctgcggc
    10561 ttatagcctt aggatcgtga actatgcgta agtggttaaa cgcgagcgcc tgtaggcttc
    10621 gctccggaga tcaccgggac ggctactgaa tccaacccag ctgcttctgg aagccgaggc
    10681 catcatagta gtcgccctga tagacgatct tgccgttctc gacccgaatg aaggtcactc
    10741 cgttgaagct gaacggcttg cccgtcgccg gcgtgtcagg cccccatgcg cccgtattgt
    10801 tgccggcgaa cgtccactca tacgcgatgc cattcgcgct ttcgatcggc tcgcccgtca
    10861 tcttccaggt gagatcaggc gccgccgtca caaacagctt gatgaccttg tcacgcgctg
    10921 catcacgccc tttctccgga tggccgacgg tggcgtcgaa ataggtgaca tcatcggcca
    10981 agagtgccgc cgcggcgtcg acgtcgtggg cattccaagc cgccatataa gcatccgcca
    11041 cctggcgcgg cgtcatatct tttgcgagcg cactcgcggc tactgccacc gtcagcaacg
    11101 cgccaatcat ccagcctcga taccacatgg tctttactcc ttctcaagcc cctgcttcgg
    11161 cacgacagcc tggcccacca aacacccggc cgcaagggcg attaagccgt acttggtacc
    11221 gggcggaaag gtcaaaattc cggtttgcgt tgcgtaaccg cgctcgcggt gcgacgctaa
    11281 agcgcaagcg gcgccgattc ttgccgcggt cgaacacggc aagattaagg ggtcagtttc
    11341 gcgcgaagcg tgaatcgcgc aaggcattgc agggcgcggc gcgtctcttt aagctgccct
    11401 tggtaggaaa tcggggaatc gcatggctca gagattgtcg aacgcaaaga gaatggcctt
    11461 agccctcgcc ggtacggcgg gtctcgtcct tggcctcggt ctcgcggcgg gaagcgacag
    11521 ggcccacgcg gtgcggctcg tgaccgccgg ccccccgagc catgcttcaa tcatagagcc
    11581 ggtgcgcatc tatcggcgcg ggcgcacgcc gatctatccc tattattaca atccgggccg
    11641 gccgggcgga tacagtttct atttcggctt cgtcccttac gcgccgggcg acatcgagaa
    11701 ccaggccctg caaaggcaat tctatccgca cagcaccgaa tggcctccgg gcctttatgg
    11761 cggaccgcaa ccgggctggg ggccgaaacg ctggcagtag ccctcgcctc gccttcagcg
    11821 cctgcttgcg aaaaacgcct tgagcaggct cgccgcctgg gtctcgccca agccgcccac
    11881 gacctccggg gcatgatggc aggtcggctg cgtgaagatg cgcgggccat gctcgacggc
    11941 gccgcccttg ggatcggccg cgccgaaata gagacggcgg atgcgggcga aagagatggc
    12001 ggcggcgcac atggcgcagg gctctagagt cacgtagagg tcgcacccgt cgagacgctc
    12061 gttgccgagc ttggcgcaag cggcgcggat ggcgagaagc tcggcatgcg cggtcgggtc
    12121 atgcagggcg agcgtatgat tgccggcccg cgccaatact tcgccgccgg cgctcaccag
    12181 aacggcgccg atcgggacct cgccgcggtc gcgtgcgcgc tcggcctctt gaagcgcgag
    12241 gtccatcggc cgcgcggcct tgccacgcat cgcctcatct gcaagcttcg atgtcatggg
    12301 cggcaagttg ggggcgaggc agaggatgcg tcaagccccg cgcggcaagg ggcgggccaa
    12361 agcgcccttg gtaagcgccg gcggcccgat gcgggtggcc aaggcgatcg ctcatgccgg
    12421 gctctgctcg cgccgcgacg ccgaggcgtt gatcgcggcg ggacgcgtcg ccgtcaacgg
    12481 cagggtgctc tcgacgccgg cgcatttggt ggcgccgggc gacaacatca ccgtcgacgg
    12541 caagcctttg ccggcgccgg aagccacgcg gctgtggcgc tataacaagc cgggcggcct
    12601 cgtcaccagc cacaaggacc cgcaagggcg aaagaccgtg ttcgaggctt tgccgcccgg
    12661 cctgccacgc gtcgtctcgg tggggcggct cgacatcaac accgagggcc tgctccttct
    12721 cactaccgac ggcgcgcttg cgcggcatct cgagcttccg agcacgggct ggctcaggcg
    12781 ctaccgcgta agggcgcacg gccgcgtca
//
LOCUS       DASUQP010000162         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1579725, whole genome shotgun sequence.
ACCESSION   DASUQP010000162 DASUQP010000000
VERSION     DASUQP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579725"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFY74_07170"
     CDS             complement(<1..413)
                     /locus_tag="VFY74_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931514.1"
                     /translation="MFWRKKDKAANSEVKIFFASDLHGSNICFKKFVNGAQFYGADVL
                     VLGGDLTGKAVIPIAEQADGTFLAFQHGDSVRINDKSELDEFVKRQGNMGFYPAVMSE
                     AEYQRLKASPEAQAALFKTLVLERVREWASFASQKL"
     gene            complement(427..1872)
                     /gene="glnT"
                     /locus_tag="VFY74_07175"
     CDS             complement(427..1872)
                     /gene="glnT"
                     /locus_tag="VFY74_07175"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEX5931515.1"
                     /translation="MNEKASGTQKKPSAGKKRETMQSLIDKGVEYCMPLYVDAHGIAK
                     TKTVPVGHFDRMMRGSELFTGAALDGLGQGPHDDELAVIPDPDAVMQLPWRPNVAVIP
                     GQLTYHEQPYPMCSRTALAKQVERAAKMGLTFNLGVECETYIVRLDPSAHNGIAPNDP
                     RDTIAKAAYDASLTLMNLEFLNEIVSAMNKLGWEVHSFDHEDANGQFEFDFAYADVMT
                     MADRFVVWRMMMKELARKYGWESTMMPKPYADRTGSGAHFNMSLADAKTGKNISGDAS
                     DRRGCGLSKLAYQFLGGLKKHAAALVAVSCPIVNSYKRLIKTGSMTGYTWAPIFISYG
                     GNNRTHMFRVPLLRPHIEGRNGHGAGHVYLSSARWECRAVDPAMNPYLAAAMFLAAGL
                     DGIEQDLDPGDPQLDNMYELSEAELEKRGVKQLPRTLLEAITAFEQDDLGRRVMGDEL
                     YRSYIDLKKGEWWSYHNAVSPWEINEYLTKF"
     gene            complement(2088..>2719)
                     /locus_tag="VFY74_07180"
     CDS             complement(2088..>2719)
                     /locus_tag="VFY74_07180"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5931516.1"
                     /translation="FARYRPSPGRVDSFLVASLELFAERNFASVTIKDIAKALDVNPA
                     LIYYYFDSKTDLFRATIERAVVRAFENVRALEDRSADPATIIAAWLANHVNKYDDIHR
                     FVKIALDFRSAHEGDPAIAKAIEAFYVEERKLLSKVIRDGVRQGLFRSVHPGRLAQFI
                     STYLDGCMVRSVILPDFDLKGAVRDLHRRVHGMLGYGGKGRKKPRRRGA"
BASE COUNT          509 a          830 c          821 g          559 t
ORIGIN      
        1 agcttctggg aggcgaaact cgcccattcg cgcacgcgct ccaggaccag cgttttgaac
       61 aaagcggcct gcgcctccgg gctggctttt agccgctgat attccgcttc ggacatgacg
      121 gccgggtaga agcccatatt gccctgacgt ttcacgaact cgtcgagctc gcttttatca
      181 ttgatccgaa ccgaatcccc atgctggaac gcaagaaacg tgccgtcggc ctgctcggcg
      241 atgggaatga cggccttgcc cgtcaggtcg ccgccaagaa cgagcacgtc ggcgccatag
      301 aactgggcgc cgttgacgaa cttcttgaag cagatattcg agccgtggag gtccgaggcg
      361 aagaagatct tcacctctga attggcggcc ttgtccttct tgcgccagaa cacatcgcac
      421 tccggctcag aatttcgtca agtactcgtt gatttcccac ggcgagacgg cgttgtgata
      481 cgaccaccac tcgcccttct tgagatcgat gtaggaccga taaagctcgt cgcccatcac
      541 gcgccgtccg agatcgtcct gctcgaacgc ggtgatcgcc tcgagcagcg tcctggggag
      601 ctgcttgacg cccctcttct cgagctcggc ctcggacagt tcgtacatgt tgtcgagttg
      661 gggatcgccg ggatcgaggt cctgctcgat gccgtcgaga cccgcggcga gaaacatcgc
      721 ggctgcgagg taaggattca tcgccggatc gacggcgcgg cactcccagc gggcgctcga
      781 gagatagaca tgccccgcgc catgcccgtt tctcccctcg atatgcggcc tgagcagcgg
      841 aacgcggaac atgtgcgtgc ggttgttgcc gccgtaggaa atgaagatcg gcgcccaggt
      901 atatcccgtc atcgagccgg tcttgatcag gcgcttatag gagttgacga tcgggcaact
      961 caccgcgacg agcgcggcgg catgtttttt cagcccgccg agaaattgat aggcaagctt
     1021 cgacaggccg cagccccgcc ggtcggacgc atcgccggaa atgttcttgc cggtcttggc
     1081 gtccgccaga gacatgttga aatgcgcgcc ggaacccgtc cggtcggcat agggcttggg
     1141 catcatggtc gattcccagc cgtatttgcg cgccagctcc ttcatcatca tgcgccaaac
     1201 gacgaaccga tccgccatgg tcatgacatc ggcataggcg aagtcgaact cgaactggcc
     1261 gttggcgtcc tcgtgatcga aggaatgcac ctcccagccg agcttgttca tcgccgagac
     1321 gatctcgttg agaaactcga gattcatcag cgtgaggctc gcgtcgtagg ctgccttggc
     1381 gatggtgtcg cgcggatcgt tcggcgcgat gccgttatgc gcggaagggt cgagcctgac
     1441 gatataggtc tcgcactcga caccgagatt gaacgtcaac cccatcttgg cggcgcgctc
     1501 gacttgcttt gccagcgccg tgcgcgagca catcggatag ggctgctcgt gataggtcag
     1561 ctggccgggg atcaccgcca cgttcggccg ccacggcagc tgcatgacag catcgggatc
     1621 cggaatcacg gccaactcat cgtcatgcgg tccctgtccg agcccgtcga gtgctgcgcc
     1681 ggtgaagagc tccgagccgc gcatcatgcg atcgaaatgg ccgaccggta cggtcttggt
     1741 cttggcgatg ccgtgcgcat cgacgtagag gggcatgcaa tattcgacgc ccttgtcgat
     1801 gagactttgc atcgtctccc gcttctttcc cgcagaaggc ttcttctgcg ttccggacgc
     1861 tttctcgttc atgggcggct gctctccttc aaggcccgca cgcgcgggaa gcgccggcac
     1921 ttgtctttgt cgttatttcg aggccacggt ctcggcgcac tcccctgccc attctccctt
     1981 cgctttgagg cctgggcaga tcaggccgtg ttccggccat ggcgttactg tgcgcgcttc
     2041 taattgaaca gtcaattagc aaaagccgtt ccacttcaac cgccgcttca cgcaccgcgc
     2101 cgccgaggct tcttgcgccc cttgccaccg taaccaagca tgccgtgcac gcgccgatgc
     2161 agatcgcgca cggcgccttt gagatcgaaa tcgggaagaa tcaccgatcg caccatgcag
     2221 ccatcgagat aggtcgatat gaattgcgcc aaacggcccg ggtgaacgga cctgaacagc
     2281 ccctgcctta cgccgtcgcg aatgaccttg gagaggagct tacgctcctc gacatagaag
     2341 gcctcgattg ccttggcaat ggccgggtcg ccttcatggg cgctcctgaa atcgagcgcg
     2401 attttcacga agcggtggat gtcgtcgtat ttgttaacgt ggttggcgag ccaggcggcg
     2461 atgatggtgg cggggtccgc gctcctgtcc tccagggcgc gcacgttctc gaaggcgcgg
     2521 acgacggcgc gttcgatcgt tgctcgaaac agatccgttt tgctgtcgaa atagtagtaa
     2581 attagcgccg gattgacgtc gagagctttg gcaatatcct tgatcgtgac cgaggcgaag
     2641 ttgcgctcgg caaagagctc gagcgaggcg acaagaaagc tgtcgacacg gccggggctt
     2701 ggccgatagc gcgcaaagc
//
LOCUS       DASUQP010000163         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_217164,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000163 DASUQP010000000
VERSION     DASUQP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217164"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFY74_07185"
     CDS             complement(<1..835)
                     /locus_tag="VFY74_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX5931517.1"
                     /translation="MGSQDKGKVNRLAGLLEEGLLASTSWLEEHEYSRPLLGKYVESG
                     WLESPARGVYRRPGPPLKWQHAVASLQLLQGSYFHVGGKTALVQRGLGHYVQMGGTPP
                     ILLYGPEVLPSWANKLGLKERFFARPDAMFGALRAWRDEKGVLRDFGDRPVDARRLDE
                     MGLREVPWGAWDWKLVHSTEERAMLELLQDVPARESVYEADVLLQGLVNLRPQRLMAL
                     LKACSSVKVKRLFLALAERHAHAWFKHLQPERLDLGSGKRMLVPGGKLHPKYLVTLPA
                     DL"
     gene            1036..2124
                     /locus_tag="VFY74_07190"
     CDS             1036..2124
                     /locus_tag="VFY74_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX5931518.1"
                     /translation="MRGHVDPQASMFSYFSPESRVPATHPLRSIKRDADAVLASLSGE
                     LDELYAEEGRPSIPPERLLKASLLIALYSVRSDRMLCEMLEYNILFRWFLDMSLEERG
                     LDQSNFSRLRERLVKLDLACRFFDAVVSEARRRKLLSDEHFTVDGTLIEAWASTAKSL
                     RRKDGSDDDSGPDDQGLVNFRGERRSNDTHESATDPEAKLKRKGLGKETKLSYGGHAL
                     MENRNGLCVDIEVRSALEREIDVAKDILSRQSRKRVRPSTLGADKGYHIKEFVSHLRT
                     RKIAPHVAQMKGRNTAGLDGRTTRHAGYAISQQKRKRVEEIFGWMKAYGGLRRPMARG
                     LKRVQLHAYIVGAAYNLLRMSRLRPATG"
     gene            2448..2546
                     /gene="ffs"
                     /locus_tag="VFY74_07195"
     ncRNA           2448..2546
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFY74_07195"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          527 a          876 c          845 g          459 t
ORIGIN      
        1 ccaggtcggc gggcagcgtg accagatact tcgggtgcag cttcccgccg ggcaccagca
       61 tgcgcttgcc gctgcccagg tcgagccttt ccggctgcag gtgcttgaac caggcgtgcg
      121 cgtggcgttc ggccagcgcg agaaacaggc gtttcacctt cacgctcgag cacgccttga
      181 ggagcgccat cagccgctgc ggccgcagat tgacgaggcc ttgcagcagc acgtcggcct
      241 cgtacaccga ctcgcgcgcc ggcacgtcct gcagcaactc gagcatcgcg cgctcctcgg
      301 tggaatgcac gagcttccag tcccacgcgc cccacggcac ctcgcgcagg cccatctcgt
      361 ccagcctgcg cgcgtcgacc gggcggtcgc cgaaatcgcg cagcaccccc ttctcgtcgc
      421 gccaggcgcg caacgccccg aacatcgcgt ccggccgcgc gaaaaagcgc tctttcaagc
      481 cgagcttgtt ggcccaggac ggaagcacct cgggcccgta gagaaggatc ggcggcgtcc
      541 cgcccatctg cacgtagtgg ccgagacccc gctgcaccag cgccgtcttg ccgccgacat
      601 ggaagtagct tccctggagc aattgcaggc tcgccaccgc gtgctgccac ttgagcggcg
      661 ggcccggacg ccggtaaacc cctctcgccg gagactcgag ccagcccgac tcgacgtact
      721 tgccgagcag agggcgcgag tactcgtgct cctcgagcca cgaggtggac gcgagcaacc
      781 cttcctccag gaggcctgcc aggcggttta cttttccctt gtcttgtgaa cccatggttt
      841 acaaaatact ccttctccaa accacgggca agtttgaaag tccgcccttt tgttaaccga
      901 cagtgtacgg ttcctgggaa ccctaaaccg gcccgccctc cgccatggaa tctagcaggt
      961 tgatgcaaaa gtccccgaac caaccgatgc gcgagtagtc ttatgcgaca acaagttggt
     1021 aagaggaaaa taagaatgcg cggacacgtt gacccccagg cttcgatgtt cagctacttc
     1081 tcgccggagt cgcgggtgcc ggcgacccac ccgctgcgct cgatcaagcg cgatgccgac
     1141 gcggtgctgg cctctttgag cggggagctg gacgagctct acgccgagga gggtcgccca
     1201 tcgatcccgc cggaacggct gctcaaggcg agccttctga ttgcgcttta ctcggtgcgc
     1261 tccgatcgga tgctgtgcga gatgctcgag tacaacatcc tgtttcggtg gttcctggac
     1321 atgagtctgg aggagcgggg gctcgaccag tcgaacttct cgcggctgcg cgagcggctc
     1381 gtgaagttgg acctcgcctg tcggttcttc gacgcggtgg tgagtgaagc gcgccggcgc
     1441 aagctcctgt ccgacgaaca cttcaccgtt gacgggacgc tgatcgaggc ttgggcctcg
     1501 actgcgaaga gcctgcggcg caaggatggt tcggacgatg acagcggacc ggacgatcag
     1561 ggcctggtca acttccgcgg cgagcgccgc tcgaacgaca cgcatgagtc ggccaccgat
     1621 cccgaggcga agctcaagag gaagggcctg ggcaaggaaa ccaagctgag ctatggcggc
     1681 catgccctca tggaaaatcg caacggcctg tgcgtcgata tcgaggtgcg ctcggcgctc
     1741 gaaagagaga tcgatgtcgc caaagacata ctctcgcgcc aaagccgcaa gcgcgttcgg
     1801 ccgagcacgc tcggtgccga caagggctat cacatcaaag agttcgtctc gcatctgcgc
     1861 acgcgaaaga tcgccccgca tgttgcgcag atgaaaggcc gcaacaccgc agggctcgat
     1921 gggcgcacca cgcgccatgc gggctacgcg atcagtcagc aaaagcgaaa gcgcgtcgaa
     1981 gagatcttcg gttggatgaa ggcctatggc ggcctgcgcc gcccaatggc gcgcggcctc
     2041 aaacgcgtgc agctgcatgc ctatatcgtt ggcgccgctt acaacctgct tcgcatgagt
     2101 cgattgcgac cagcgacggg gtaattccgt ccaaacaggc acaagatgag caaccaaacg
     2161 caaaaccgag cggttcagag cataaaagcg ggtcaccgga tcaaccgggt gtcgatcggc
     2221 gcgcagaatt cgctcatgtc tcatcttttt gcatcaacct gctagaagaa aacgccccgc
     2281 ggcttgcgcc gcggggcggt agaagccatc gcgcgtttcg acgcgatgga ggaggatgca
     2341 gcgcttcgac gatagctcgc gccgccatcg cgccgctttc ggcaagctga ctgcggcctt
     2401 gcagcgcgga gcgtttcaca ccgcatgaat gacttacaat tggacccggc gggcctcccc
     2461 gcattgcagt ctggtgaacc tggtcaggtc cggaaggaag cagccacagc cggccgctgc
     2521 aagtgccggg gatcaggctc gccacgaatt gactaaacga tgagctatca agtacttgcc
     2581 cgcaaatggc ggccgcggga ttttggcagc ctggtgggtc aggagcacat cgtgcgcgcg
     2641 cttcgccacg cgctcgaggc gaggcggctg caccacgcgt acctcttcac cggtactcgc
     2701 ggcgtgg
//
LOCUS       DASUQP010000164         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_898717,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000164 DASUQP010000000
VERSION     DASUQP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898717"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            512..1132
                     /locus_tag="VFY74_07200"
     CDS             512..1132
                     /locus_tag="VFY74_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5931519.1"
                     /translation="MPRPAPLANRRNVKSAAGCAADAHERRRDFLGEAEIAALLDAAR
                     RGRHGARDHLLILMMYRHGLRVSEAIALRRGDANLKEARLWIARLKNGLSVEQPIAGD
                     ELRAIKRWLGIRRDHLPWLFLSERGQPLSRQSVNYIVAAAAKRADLAPVNPHMLRHSC
                     GFALANRGYDLRLIQDYLGHRDPKHTARYTRIAAKRFDGLWGAGAN"
     gene            1149..2561
                     /locus_tag="VFY74_07205"
     CDS             1149..2561
                     /locus_tag="VFY74_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931520.1"
                     /translation="MPPSDSRSGLMKWLRREPWSSAFGELLERHLGPACDKAGVPLEG
                     LSEVIGDHWAMTLWGCAFEDFLTREVEGVGNIVDDYLKRRGWNETAMNRAYMTALRSS
                     VISLYEASDIRPGESFLARDLIRGGEPVRVSERTATRTLKPWDRIAARMVEVRGKTTL
                     GGGVLPFEHELSKSLIAALDRILARAPGELADLVADLGIELSPEESASVIDEAEMLRL
                     AAPMVSTYWLNDTLERVLSPAVPKVYNSDGEEMEIFALHYRLEEGVTAEKLRAALGAA
                     PGLDPASATFWNWLAPGDGPGTRVKPKARDGLTFTSTMAQGAVVLGTLELNNRTLTLQ
                     VNSEPRAERGRAMLDPLLEGLVSAPLIERQTLEQAMAEHRTRGSAPVSSGLPPDEERR
                     LIHAQLDAHYRQQLDAPIPALGNISPRTAAKTAEGREKIASWLKLIENHAARQGPGDP
                     MGDYDLTWMWQELGVADLRK"
BASE COUNT          511 a          969 c          942 g          469 t
ORIGIN      
        1 cgtccgcgag gccgccgcgc ggctcagggt cggcaagacc gcgctctacg aggcgctcgc
       61 ggcccgggag acggccagcg cgtgagttcg cccgccgctt tcagccggcg tacgtaaaac
      121 gcactttcgt gcggtgaccc cttccaggga cgccgccgcc tgcggatcag gccgatgtcg
      181 tgatcgctga ggacgtagaa ccggaggtgc gtcgcctcgt cctcgggcag ggcgaacagc
      241 gccgcccgtt ggtgctcggt gaggatcgtg cggtgcgcca ttgcgccgac gcttcatctc
      301 cttggatcgt ggtggcgggg tgagagcagg agccctcgaa ccgcgtcaac ccgtacggcg
      361 tctccgagct cgacctggat cggcgttcgg acatcgagct gccgacggcg acgcggacgg
      421 ccgggcgtga gccggggcaa cgggcgactt tttacgaaat gcgcgttgtg taagaagtac
      481 cgatcggccg tccccgagcg agcagaggct tatgccccga ccggcgcccc tcgccaaccg
      541 gcgaaatgtc aagagtgccg ccggctgcgc cgccgacgcg catgagcggc gcagggactt
      601 cctcggtgag gcggagatcg cggccctgct cgatgccgcg cgcaggggtc gccacggcgc
      661 ccgtgatcac ctgctgatcc tgatgatgta ccgccatggc ttgcgggtct cggaggcgat
      721 cgcgttgcgc cgcggggacg ccaacctgaa ggaggcacgc ctttggatcg cgcgcctgaa
      781 gaacggcctc tcggtcgaac agccgatcgc cggcgatgag ctgcgcgcca tcaagcgctg
      841 gttgggtatc cgccgggacc acctgccctg gctcttcctc tcggagcgcg gacagcccct
      901 gtcccgccag tcggtgaact acatcgtggc cgcggccgcg aagcgcgcgg acctggcgcc
      961 ggtcaacccc cacatgctgc gccattcctg cggcttcgcg ctcgccaacc ggggctacga
     1021 cctccgcctg atccaagact atctcggcca ccgcgacccc aagcacaccg cccgctacac
     1081 ccgcatcgcc gccaaacgct tcgacggcct ctggggcgcg ggggcgaatt gaagaggagc
     1141 gacccgtcat gcccccatcc gactcccgct caggcctgat gaaatggctc cgccgcgaac
     1201 cctggagcag cgcgttcgga gaactactgg agcgccacct gggccccgct tgcgacaagg
     1261 cgggcgtgcc gctcgaagga ctgagcgagg tgatcggcga tcactgggcc atgaccctct
     1321 ggggctgcgc cttcgaggac ttcctgaccc gtgaggtcga aggcgtgggc aacatcgtcg
     1381 acgactacct caagcgccgt ggctggaacg aaacggcgat gaacagggcc tacatgaccg
     1441 ccctccgctc ttcggtgatc agcctctacg aggcgagcga catccgcccg ggcgagtcct
     1501 tcctcgcccg cgatctgatc cgcggtggcg aaccggtgcg ggtcagcgag cgcacggcga
     1561 cgcgcacgct gaagccctgg gaccggatcg cggcgaggat ggtggaggtg cgggggaaga
     1621 cgaccctggg tggcggggtg ctccccttcg agcacgagtt gtcgaagagc ttgatcgcgg
     1681 cgctcgaccg catcctggcg cgtgcgcccg gggaactcgc ggacctcgtc gccgatctgg
     1741 gtatcgagct gtcgccggaa gaaagcgcct cggtgatcga cgaggccgag atgctgcgcc
     1801 tcgccgcccc gatggtctcg acctactggc tcaacgacac cctcgagcgc gtgctgagcc
     1861 cggccgtccc caaggtgtac aacagcgacg gcgaagagat ggagatcttc gcgctgcatt
     1921 accgcttgga ggaaggcgtg acggccgaga agctccgcgc cgcgctcggt gccgcgcccg
     1981 gtctcgaccc ggcttcggcg accttctgga actggttggc gccgggagac ggccctggga
     2041 cccgcgtgaa gccgaaagcc cgcgacggcc tcaccttcac ctcaacgatg gcgcagggcg
     2101 ccgtcgtcct cggcacgctc gagctgaaca acagaaccct cacgctccaa gtgaattcag
     2161 agccccgcgc cgagcgcggc cgcgcgatgc tggaccccct gctggagggg ctcgtctccg
     2221 cgccgctgat cgaacgccag acgctcgagc aggccatggc cgagcatcgg acacgcgggt
     2281 cggcacccgt gtcctccggc ctgccccccg acgaggagcg tcgtctgatc catgcccagc
     2341 tcgacgcgca ctatcgccag cagctcgacg cccccatccc ggcgctgggt aacatcagcc
     2401 cgcgcacggc cgccaaaacg gccgagggcc gcgagaaaat cgcttcgtgg ctcaagctca
     2461 tcgaaaacca tgccgcgcga caaggtcccg gcgacccgat gggcgactat gatcttacat
     2521 ggatgtggca agagctcggt gtcgccgatc tgcgcaaatg acctgagctg tttgcgcaaa
     2581 tcatcgccaa tctctaatga cccgtttggc tgttctgcac agcttcgtgc tcactgagct
     2641 actttgctta gagcccgtcc gggaatgggg catttggttt caagccactc agcggcgtat
     2701 cgccagacac gcggcattaa aataaaattt tagcaaaatg acatcaaaat aaaacttaga
     2761 aaaaacagtt atattatgca gaatttaagg aaacggaaga ttttgacatg ggtcagacat
     2821 gctcggtaat cttctttggc gcgtcggcct cggtcgtatc agagcggcct tggcaattcg
     2881 acggtgcttt t
//
LOCUS       DASUQP010000165        18208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_899085,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000165 DASUQP010000000
VERSION     DASUQP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18208
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899085"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..487
                     /locus_tag="VFY74_07210"
     CDS             59..487
                     /locus_tag="VFY74_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5931521.1"
                     /translation="MTSEALTAEDVIRQHALKPHPEGGWFRETFRDETGPHGRAHSTA
                     ILFLLKAGEVSRWHKVDAAELWHWYGGAPLLLEVKHGGTRHAHRLGPDWHKGDHPHVA
                     VPARAWQSARSLGAWTLLGCTVAPGFDFAGLHLAPDDFES"
     gene            complement(804..1340)
                     /gene="ppa"
                     /locus_tag="VFY74_07215"
     CDS             complement(804..1340)
                     /gene="ppa"
                     /locus_tag="VFY74_07215"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX5931522.1"
                     /translation="MRLEAIAIGLNPPDDLNVIIEVPIGGEPIKYEMDKAAGTLVVDR
                     FLHTPMRYPGNYGFVPHTLARDGDPLDVLIANTRPIVPGAIINVRPIGVLKMEDDSGM
                     DEKIIAVPTSKLTKHYEHVRTYADLPKIAQEQIQHFFEHYKDLEPGKWVKLIGWGNVE
                     EAKQLIIEAIQRERHSPR"
     gene            complement(1637..2359)
                     /gene="phbB"
                     /locus_tag="VFY74_07220"
     CDS             complement(1637..2359)
                     /gene="phbB"
                     /locus_tag="VFY74_07220"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX5931523.1"
                     /translation="MGRVALVTGGTRGIGAAISKALKQAGYNVAANYGGNDDAAKAFS
                     DETKIPAFKWDVGDYAACEEGIVNVQNEVGPIEVLVNNAGISRDAMLQKMTHEQWEQV
                     MRTNLDSLFNMTRPIINAMREKGFGRIINISSINGQKGQLGLANYCSAKAGVVGFTRA
                     LALESARKGITVNAIAPGYVNTELLSGVSDEVMKSIVGQIPVGRLGEADEVARCVVFL
                     AADEAGWITGSTLTINGGQFMD"
     gene            complement(2429..3610)
                     /locus_tag="VFY74_07225"
     CDS             complement(2429..3610)
                     /locus_tag="VFY74_07225"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5931524.1"
                     /translation="MKEEEIVIVSAARTPVGSFNGALASLPAHTLGEVAIRAALERAK
                     VDPTDVDEVIMGQVLTAGEGQNPARQAAMAAGVPVEATAWGVNQVCGSGLRAVALGAQ
                     QIAAGDADIVVAGGQESMSLAPHVAHLRNGQKMGAMEFIDSMIKDGLWEAFNGYHMGN
                     TAENVARQWQITREEQDKFATHSQNKAEAARKAGKFKDEIAPVTVKTRKGETVVAEDE
                     YIREGAKVEDAAKLRPAFDKEGTVTAGNASGINDGAAVVVLMTAEEAKKRGLKPLARI
                     ASWAVAGVDPKIMGSGPIPASRKALKKAGWNVGDLDLIEANEAFAAQALAVNKDLAWD
                     SGKVNVNGGAIAIGHPIGASGARVLVTLLHEMQKRDAKKGLATLCIGGGMGIAMCVER
                     D"
     gene            3804..4415
                     /gene="phaR"
                     /locus_tag="VFY74_07230"
     CDS             3804..4415
                     /gene="phaR"
                     /locus_tag="VFY74_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX5931525.1"
                     /translation="MARDAETESRPVIVKKYANRRLYNTDTSTYVTLEDLAEMVRSER
                     DFVVYDAKTGEDLTHSVLTQIIVEQESRGTNLLPIGFLRQLIRFYGDSMQKLVPSYLE
                     FSLDSLTRQQEQYRRRFAHVFGTAAFEAMQEQVRKNFETFERALGMFSPFTSADAGEG
                     SNADQPEKSEGKPEIGKPSEDEIANLKAELTAMQQRLEQLSRR"
     gene            complement(4446..5177)
                     /locus_tag="VFY74_07235"
     CDS             complement(4446..5177)
                     /locus_tag="VFY74_07235"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX5931526.1"
                     /translation="MTEERIRLSEPVPAGFERPSRGPRAISAYIRRAIETGAYSEGDR
                     LPPERQLAATFQAARSTVRRALDQLEKAGLVSRRLGSGTFVGASGASARRSVDLADQV
                     SPLQLIEARLAVEPFTTRLAVFHATRRNLDDMEAVLVHAEESVNDKDSFSKWDGEFHL
                     LIAHASGNPLLINVYRQINHVRLHAQWDAMKEKILSPDVIVDYNRQHRAIFDALNARD
                     AQGAHALITQHLEKARDDLLKANSP"
     gene            5342..6685
                     /locus_tag="VFY74_07240"
     CDS             5342..6685
                     /locus_tag="VFY74_07240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5931527.1"
                     /translation="MSVPQGVKFAIIGAGVHGLSTAFHLAQMLKATGRGSGADVLVVD
                     KTAVAAGASGIACGVVRNNYFQPAMRELMAHSVEVWESDAKAYSYHPVGYMQISPESM
                     HADVASIYEQQQAIGYESAFIEGADDSMRYMKGLFDDWQAQGITSVLHEKRGGYANNQ
                     ASMQGLAAKATEEGVRILSGVEVKSFEFGSNSGAVTALNTNQGRIACDTVVVGAGPWV
                     NKIWNLLELPKKITVKANGKVRDDVPMWKFWCLEEGTLGVDPHMHRTNDGRFPPVLHV
                     DTDAPLYSERDGKLITDKIWGIYYKPDFNFGGVQGGASPYKVDQDPDRIAVDPYGPES
                     PEFVVGEDFIDMWCSALAHCQKRFEGQMPLYKRDENSGGLGCFTPDNFPVFDVFRENV
                     YVIADSNHGYKMIGVGKLVAQELLGEKSRLLEPFRFARFAKGELHPTSHSPFPWS"
     gene            6737..8260
                     /locus_tag="VFY74_07245"
     CDS             6737..8260
                     /locus_tag="VFY74_07245"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007816356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX5931528.1"
                     /translation="MDRRVETKKSSKANRSKPTDIEQYVEAPGRKALVAEVRKKIDAL
                     GIEYLYLQFISVTGRIMGKGIPADHWESIAQKGFQLVYGATMNLFLNRRGDYLGYGPE
                     AAELVGIPEPETFMQLPWDKRVARLWCTLFRNREEREDPGAFLTADSRGNLRRLHEQF
                     QKDHGLQLRHGTEPEMMWLKKGADGEPAGGYSNPYCYHIDQFESLRLVYMQVIDYCRK
                     MGLDMIQGDHEDSPGQLELNFTFDDALRTADRLATYRQICAQVAREFGIIACFMCKPF
                     MGVSANGCHHNISLWRGGKDELKSLGNDPKKLPGMAQNYMYSKGGDNTFMPDTDDVQL
                     PGKIGLHAIGGIVKHLGALTAIGCSTVNSYRRLWDTGFWAPVFSDWGFQNRTTGLRVS
                     APGRFEYRAVDAMVNPHLMAAALIMAMDDGIKNKLDPGPSEERNIYQAMEAGKQVKRL
                     PMTLGDALAALEKDPVIRSAMPGEMYRLYDEYKRDEWERFLHTTTEWDIKTYMDCLP"
     gene            8291..9226
                     /locus_tag="VFY74_07250"
     CDS             8291..9226
                     /locus_tag="VFY74_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX5931529.1"
                     /translation="MCGIAGLIHRDGVTSVGREMTAMLKSLKHRGPDSTGFAVYGASN
                     PNEYVLRLKLAEQEDMTKDHHIRRKIRARQSEVDKRLAELGVETLEVAEATPYAFRYR
                     LRYAGDTRRLTTEIEQIEGAEVLSFGSALELIKDLGDATQVSEQYGLGTFRGTHGIGH
                     TRMATESDVDIRSAHPYWAYPYNDIAVVHNGQITNYWIMRREMERLGHRFMSNCDSEL
                     LAVYTANNLEKGATLEDSLKASLKDIDGVFTYVVATENELGMAKDTMAAKPMVLFESD
                     NLVALASEEVAIRAIMPREIDTFDPYDEEIRVWQR"
     gene            9214..10086
                     /locus_tag="VFY74_07255"
     CDS             9214..10086
                     /locus_tag="VFY74_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GXGXG motif-containing protein"
                     /protein_id="HEX5931530.1"
                     /translation="MAEIARSELSEQERRSFEMGLHTELLKGRSHQVFFDFDLKEAPH
                     GFALPNAYDVDFNKRAEIDAADLATNAINLRIRELMDDGYGTIVVRNPGAKHSIAVGI
                     LNRLNLIIDGSLGYFGCGLIDGPNVRIAGRVGWSCAENMMSGTILIEKNAGSQFGAAI
                     RGGDLVCRGNVGSRTGIDMKGGTIIVGGDTGAFTGFMMQRGRMVICGNAGKNLGDSMY
                     DGTIFIAGEPQSLGVDAVWGTMTELDKSWLENKLRRYELLPKEGIGAFRKIVAGKQLW
                     NYDNLEPTEKKLVL"
     gene            10109..11494
                     /locus_tag="VFY74_07260"
     CDS             10109..11494
                     /locus_tag="VFY74_07260"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX5931531.1"
                     /translation="MKKNRNGKNGKGSVKAPAEAERFILGRSQIFTPDVINDIHVKSE
                     LGRYRMRGFSLFKKIPHWDDLTFLPGTLTRFVIEGYREKCDTKTVIGPRAKRPMVLDI
                     PVYVTGMSFGALSYESKIALARGATMAGSATCSGEGGMIPGERRYSIKWFYQCIQSRY
                     GFNPHHLRLADACEFFIGQGCKVGLGGHLMGQKVTDQVAEMRSLPAGIDQRSPARHPD
                     WLGPDDLALKIEEVREATNWEIPIQLKLGAARVYDDVRMAVKCGPDSIYIDGMEGGTG
                     AGPHIATEDTGVPGIAAIRQARRAIDELGKRGEISLVYAGGIRNGGDVAKALALGADA
                     VAIGHSVLMALNCNKEIPESDFENEIGVPAGYCYHCHTGRCPVGVATQDPELRKRLDP
                     DAAAERVYNFLHTLTLEAQMLARACGKTNVHSLEPEDLAALTMEASAMAQVPLAGTDY
                     TVGVENYHKIG"
     gene            11509..11934
                     /locus_tag="VFY74_07265"
     CDS             11509..11934
                     /locus_tag="VFY74_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931532.1"
                     /translation="MSGKSSKDAKKSKAKNNVDKYLKDPSGIDTAREKQIGQHIGYRY
                     DVNLLPDYARLTPYLKTYIEFMGWDDLNWLEDVHMGYEAGKPAIFDRNINGWVRIPKD
                     MKLPDNQQDRDMLARELLVRFQTSPNHPLVQLNKAYRKF"
     gene            12048..12293
                     /locus_tag="VFY74_07270"
     CDS             12048..12293
                     /locus_tag="VFY74_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931533.1"
                     /translation="MKQWLLKLMLPARQADRPNFIASSLGAGADVWGTGTLTAGIVAI
                     ALIRSGVRLPALRPGWGRLPRRDARGLHAHGREDWGA"
     gene            complement(12426..12980)
                     /locus_tag="VFY74_07275"
     CDS             complement(12426..12980)
                     /locus_tag="VFY74_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931534.1"
                     /translation="MTPSAANTIKHEFLAWQCRLRQIAMRQDGGRPSPGMRPRLLDRD
                     GREIARALTVLLIPQDPAESTAFFRFQSARFPDPQELYERALRFLQADYFQQPENFSD
                     KLVAVLPEGLPLAAQLNAQGRCILDFAQFNQTYRLPCTVFELEPGEAAREAAIWHNRQ
                     FNPALPDTVAVLQFRPDWTSAGTR"
     gene            13326..14726
                     /locus_tag="VFY74_07280"
     CDS             13326..14726
                     /locus_tag="VFY74_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5931535.1"
                     /translation="MEGNLNALTTIFTEFYYWVTIVFMFFIHVGFCTYEVGVSRRRNH
                     LKTLMKNAMAIPLVTVTFFFFGWWIYFAFPNGPGITGGLIPAPYSEPWNPLMAPHLSG
                     VNPNEAGSVTPAEALLWHRLNGVFWGAFLLFSITTASIISGSIIERVRSGAFWVIAVL
                     IGSVMWILDAAWGWHPLGWMVKLWGYHDAYASGVVHAIAGGAALAFLIVLGPRIGKFR
                     PDGTPRDIPPHNEWLVVIGLFLIYTGFWGFYAACNIPIISPATIAGQITGDTWTATNI
                     YLAPTTLSAITFNFLMSLSGGMMGGYLFSRGNPFWTFSGGLCGIISASAGNDLYHPIQ
                     AMLIGAIVPVICFKLHYFVERRFKIDDAVGAVAVHGYGGFLGVVVAGFMLWGQPSSPY
                     EGYAHINPLGNFAGACLMFLLGFVPTYIACMIMNAMGLLRVPAKVELEGVDFALNHTF
                     MSSVREVGSAEKAMIK"
     gene            14747..14968
                     /locus_tag="VFY74_07285"
     CDS             14747..14968
                     /locus_tag="VFY74_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931536.1"
                     /translation="MSTNGMTSWAVDLKDVGAIYPFQGTEVLLVILGLAFWIGWHILQ
                     TRQETAEINSDMAADRSGDETRSAIDRYY"
     gene            complement(15046..15501)
                     /locus_tag="VFY74_07290"
                     /pseudo
     CDS             complement(15046..15501)
                     /locus_tag="VFY74_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995969.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(15510..16814)
                     /gene="glnT"
                     /locus_tag="VFY74_07295"
     CDS             complement(15510..16814)
                     /gene="glnT"
                     /locus_tag="VFY74_07295"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEX5931537.1"
                     /translation="MAVDLAATARERGIKYFLISYVDLFGTLRAKLAPASAIADMAKD
                     GAGFAGFASWLDMTPAHPDVLAIPDPASLIQIPWKPEVGWLAADPYMDGKLVEQAPRN
                     ILKKMIAKAAKAGFQLKTGVECEFFLLSPDGSTISDASDTQVKPCYDQSALMRRYDVI
                     SEICDAMLELGWGAYQNDHEDANGQFEMNWAFDDALVTADRMAFFKYMVKAIAEKHGL
                     RATFMPKPFANLTGSGCHVHASLWQGNKNVFDDDKDALGLSRTAYHFIGGLMNAAGDI
                     CAITNPTVNSYKRINAPVTLSGATWSPNTVTYAGNNRTHMIRIPGGGRFEFRLADGAA
                     NPYLLPAAILAAGLDGIARSIDPGKPLDIDMYAEGHKAPPGTKKLPLNLLDALRMTQK
                     SDLLRRELGEEFVDSYVKLKMREWNDYAGHLSAWERAATLDC"
     gene            complement(16840..18171)
                     /locus_tag="VFY74_07300"
     CDS             complement(16840..18171)
                     /locus_tag="VFY74_07300"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX5931538.1"
                     /translation="MDWTPRTAPRPSATFDASSLSEIRRAAATGIYDIRGAGAKRKLP
                     HFDDLLFLGASISRYPLEGYRERCSTDVWLGTRFAEKPIHIKIPVTIAGMSFGSLSAQ
                     AKEALGRGASQLGTSTTTGDGGMTEEERHHSKTLIYQLLPSRYGMNPDDLRRADAIEI
                     VVGQGAKPGGGGMLLGQKISDRVAEMRTLPKGIDQRSACRHPDWTGPDDLEIKIEEIR
                     EITDWQKPIYVKVGASRPFYDTALAVKAGADVIMLDGMQGGTAATQEVFIEHVGLPIL
                     GAIRPAVDALQDLGMHRKVQLIVSGGIRNGADVAKALALGADAVAIGTAALVALGDND
                     PALEEEYRKLGTTAGCYDDWHEGRDPAGITTQDPTLAARLDPVLAGRRLANYLSVLTL
                     ETQTISRACGKSHVHNLEPEDLVALTVEAAAMARVPLAGTNWIPGANSSSE"
BASE COUNT         3383 a         5668 c         5780 g         3377 t
ORIGIN      
        1 gaaatctttg gcgagatcag gcgccgcaag gatcggttct gacgcgtatc ttttgatcat
       61 gaccagcgaa gcgctgaccg ccgaggatgt catcaggcag cacgcgctca agccgcatcc
      121 cgaaggcggc tggttccgcg agacgttccg cgacgagacg gggcctcatg gccgcgcgca
      181 ttcgaccgcg atcctgttcc tcttgaaagc gggcgaggtg tcgcgctggc acaaggtcga
      241 tgcggccgag ctgtggcact ggtatggagg ggcgccgctc ctgctcgaag taaagcacgg
      301 cggaacccgc cacgcccatc ggctcggtcc cgattggcac aaaggcgacc acccgcatgt
      361 ggcggtgccg gcgcgtgcct ggcagagcgc gcggagcttg ggcgcgtgga cgctgctcgg
      421 ctgcaccgtt gcgccgggct tcgatttcgc cggcttacac ctcgcccccg acgatttcga
      481 gtcctagcct ctcgcgccta acctccccct ggtggggagg tcggtacgcg gccctgtttt
      541 tgcgctaccg ctccgctact tgagcgcgcg gcgcgccggg tggggggaaa atccgagtgt
      601 ccgttttgcg gaccgccatc gacgttgttt tcgacagagt cctgcacgag ttgacacgca
      661 agccccccac cccgacgccg caagtaatct ttgcgctacc gcttcgctac ttgagcgcgt
      721 tgcgtcgtcg gacctcccca cgagggggtt agaaatgaaa gcggcgccga catcacacca
      781 gggcggggca ggaggtatgg gggtcagcgg ggagagtgcc gctcgcgctg aatggcctcg
      841 atgatcagct gcttggcctc ttcgacattg ccccagccga tcagcttcac ccacttgccg
      901 ggctccagat ccttgtaatg ctcgaagaag tgctggatct gctcctgcgc gattttcggc
      961 aggtcggcat aagtcctgac atgctcgtaa tgcttcgtca gcttcgaggt cggcacggcg
     1021 atgatctttt catccatgcc gctgtcgtct tccatcttca gcacgccgat cgggcgcaca
     1081 ttgatgatgg ctccggggac gatcggccgc gtattggcga tgagaacgtc gagcgggtcg
     1141 ccgtcgcgcg ccagcgtgtg aggcacaaat ccgtaattgc ccggataccg catcggcgtg
     1201 tggagaaagc gatccacgac gagcgtgccg gcggccttgt ccatctcgta cttgatcggc
     1261 tcgccgccga ttggcacctc gattatgaca ttgaggtcat cgggagggtt caggccgatg
     1321 gcgattgcct cgagacgcat ggggtgactt accgatcctg gtacggactg cgcggcatat
     1381 gctggcgaac ctaagccccc caccccggcg tcgcaagcgc gacgccggac ctccccacga
     1441 gggagaggtt aaatatcacg tgcgacatcg gggcttcgga ttggcgcgac aacttctggc
     1501 cctaggctct agccggcaaa cctcccccct cgtggggagg tcggcgcgac gctctattcg
     1561 cgctactctc cgctgcttta gcgcggcgcg ccgggtgggg ggacattgcg ttcggagttg
     1621 agaggctccg gcgcgatcaa tccatgaact ggccgccatt gatggtgagc gtcgagccgg
     1681 tgatccagcc cgcctcgtcg gcggcgagaa acacgacgca gcgagcgact tcgtcggcct
     1741 cgccaaggcg tcccaccgga atctggccga cgatcgactt catcacctcg tcggaaacgc
     1801 cggatagcag ctcggtgttc acgtagcccg gcgcgatcgc gttgacggtg atcccttttc
     1861 gcgcgctctc cagcgccagc gcccgggtga agccgaccac gcccgccttg gccgaacagt
     1921 aattggcgag cccaagctgg cccttctgcc cgttgatcga ggagatgttg atgatgcggc
     1981 cgaagccttt ctcgcgcatg gcgttgatga tcggtcgcgt catgttgaac agcgagtcga
     2041 gattggtgcg catcacctgc tcccactgtt cgtgcgtcat cttctggagc atggcgtcgc
     2101 gcgaaatgcc ggcattgttg accagcacct cgatggggcc gacctcgttc tggacattga
     2161 cgatgccctc ctcgcaagcc gcgtagtcgc caacgtccca tttgaacgcg gggatcttgg
     2221 tttcgtcgct gaaggccttg gcggcgtcgt cattgccgcc atagttggcg gcgacgttgt
     2281 aaccggcctg cttcagcgcc ttgctgatgg ccgcgccgat gccgcgcgtt ccgcccgtga
     2341 ccaaagccac ccttcccatg gactcctcct tctgaaagcg cgtcgcggcg cttgatgacg
     2401 tttcaacgat ggcaatcgaa gcgcgcggtc aatcccgctc gacgcacatg gcgatgccca
     2461 tgccgccgcc gatgcagagc gtggcaaggc ccttcttggc atcgcgcttc tgcatctcgt
     2521 gcagcagcgt gacgagcacg cgcgcgcccg aggcgccgat cggatggccg atggcaatgg
     2581 cgccgccatt gacgttgacc ttgcccgagt cccaggcgag gtccttgttc accgccagcg
     2641 cctgcgcggc gaaggcctca ttggcctcga tcaggtcgag atcgccgaca ttccaaccgg
     2701 ccttcttcag cgccttgcgc gaagcgggga tcggtcccga tcccatgatc ttgggatcga
     2761 cgccggcaac ggcccaggac gcgatacggg caagcggctt gagaccgcgc ttcttcgcct
     2821 cctccgcggt catcagcacg acgacggcgg cgccgtcatt aatgcccgaa gcattgcccg
     2881 ccgtcaccgt gccttccttg tcgaaggctg gtctgagctt tgccgcatcc tcgaccttgg
     2941 cgccctcgcg gatatattcg tcctccgcca ccacggtctc gccctttcgg gtcttcaccg
     3001 tgacgggggc gatctcgtcc ttgaacttgc cggccttcct ggcggcctcg gccttgttct
     3061 gcgaatgggt ggcgaatttg tcctgctcct cgcgggtgat ctgccactga cgggcgacgt
     3121 tctcggcggt gttgcccatg tggtagccgt tgaaggcttc ccacagcccg tccttgatca
     3181 tcgagtcgat gaactccatg gcacccatct tctggccgtt gcggagatga gcgacatgcg
     3241 gggccaggct catgctttcc tggccaccgg cgacgacgat atcggcatcg cctgcggcga
     3301 tctgctgggc gccaagcgcg acggccctga ggccagagcc gcatacctga ttgacacccc
     3361 aggcggtcgc ctcgacgggc acgcccgcgg ccatcgccgc ctggcgcgcc gggttctggc
     3421 cctcgcctgc ggtcagcacc tgacccatga tcacttcatc gacatcggtg ggatcgacct
     3481 tggcccgctc gagcgccgcc cgaatggcga cttcgccaag ggtatgggcc ggcaagctcg
     3541 cgagtgcgcc gttgaaggag ccaacgggcg ttcgcgccgc gctgacgatg acaatctctt
     3601 cctctttcat actcctcgtc tccgctttcg gtggggaacc taggtcataa cttagggcgt
     3661 tcctctaggg aagcccaggt tttgccccgc attaaaggcg tggcatgaag ccactggtgc
     3721 tgtgcacaaa aatcgtgcta ctggtctcgc agttgcggaa taaagcccct tgggcgcccc
     3781 caaggcgtag gagcgatagg cacgtggcaa gggacgcaga gaccgagagc cggccggtca
     3841 tcgtcaagaa atatgccaac cgcaggctct acaataccga cacaagcacc tatgtgacgc
     3901 tcgaggactt ggccgagatg gtccggtccg agcgagactt tgtcgtttac gacgccaaga
     3961 ccggcgaaga tttgacccac tcggtcctga cccagatcat cgtcgaacag gaaagccgcg
     4021 gcaccaatct ccttcccatc ggtttcctgc gccagctcat ccgcttctac ggcgacagca
     4081 tgcagaagct ggtgccgagc tatctcgaat tcagtctcga ttcgcttacc cgccagcagg
     4141 agcagtatcg ccgcagattc gcccacgtct tcggcacagc ggccttcgag gcgatgcagg
     4201 agcaggtgcg caagaacttc gagaccttcg agcgggcgct cggaatgttc agccccttca
     4261 cctcggccga tgccggcgaa ggaagcaacg cggatcaacc ggagaaaagc gaaggcaagc
     4321 cggagatcgg aaagcctagc gaggacgaga tcgcgaatct gaaggccgag cttaccgcga
     4381 tgcagcagcg cctcgagcaa ctttcccgcc gttagccatt ctctgcctga tatcgcgcct
     4441 gcgccttacg ggctgtttgc cttcaggaga tcgtcgcgcg ccttttcgag atgctgcgtg
     4501 atcagcgcgt gggcaccttg cgcatcgcgc gcgttgagcg cgtcgaagat ggcgcgatgc
     4561 tggcgattat aatcgacgat cacgtcgggc gagagaatct tctccttcat cgcgtcccat
     4621 tgggcgtgaa gccgcacatg attgatctgg cggtagacgt tgatgagcag cgggtttccc
     4681 gaggcgtgcg caatgagaag atggaactcg ccgtcccatt tggagaagga atccttgtcg
     4741 ttgaccgatt cttcggcgtg gacgaggaca gcttccatgt cgtcgagatt gcgccgcgtc
     4801 gcatgaaaga cggcgagccg cgtggtgaag ggctcgactg caagccgcgc ctcgatgagc
     4861 tggagcggac tcacttgatc ggcaaggtcg acgctccgcc gggctgaggc tccgctcgca
     4921 ccgacgaagg ttccgctgcc gagacggcgc gacaccagtc ccgctttctc gagctgatcg
     4981 agggcgcggc gcaccgtgct tcgcgccgcc tgaaaggtcg cggccaattg ccgttcgggc
     5041 ggcagcctgt cgccctcgct ataggcgccg gtctcgatgg cgcggcggat gtaggcggaa
     5101 atcgcacgcg gtccccggct cggtctttcg aaccccgcag ggaccggctc tgaaagccga
     5161 atgcgctcct cagtcaaggt ctgcacctcc tcgaacgaga gcccactttg gacccaaatg
     5221 aaaaaattgg tttacaaccg gaccacgtct tgagctatag taatcttaca aggcttcctc
     5281 gattgggtct aggtgtttga gaccaaaacc ggaccaatcc tctagtcgcg ggagagtgcc
     5341 gatgagtgtc ccacagggcg tcaagttcgc catcatcggc gcgggcgtac acggcctcag
     5401 caccgccttc catctggctc aaatgctgaa agcaaccggc aggggctccg gcgccgacgt
     5461 tctcgtggtc gacaaaacgg ccgtggcggc gggcgcctcg ggcattgcct gcggcgttgt
     5521 gcgcaacaac tatttccagc cggccatgcg cgagctgatg gcccactccg tcgaggtgtg
     5581 ggagagcgac gccaaggcct attcctatca tcccgtgggc tacatgcaga tcagccccga
     5641 aagcatgcac gctgacgtcg cctcgatcta tgagcagcag caagcgatcg gatacgagtc
     5701 ggcgttcatc gagggcgccg acgattcgat gcgatacatg aagggcctgt tcgatgattg
     5761 gcaggcgcaa ggcatcacct ctgtgctgca tgaaaagcgt gggggctacg ccaacaatca
     5821 ggcgagcatg cagggccttg ccgccaaggc cacggaagag ggcgtgcgca tcttgtccgg
     5881 cgtcgaggtg aagagcttcg agttcggctc aaatagcggc gcagtcaccg cgctcaacac
     5941 caatcagggc cggattgcct gcgatacggt cgttgtcggt gccggtccct gggtgaacaa
     6001 gatctggaac ctgctggagc tgccgaagaa gatcaccgtc aaggccaacg gcaaggtccg
     6061 cgacgacgtg ccgatgtgga agttctggtg cctggaggaa ggtaccctcg gcgtcgatcc
     6121 gcacatgcac cggacgaatg acgggcgctt ccccccggtg ctgcacgtcg ataccgacgc
     6181 gccgctctat tccgaacgcg acggcaagct catcaccgac aagatctggg gcatctacta
     6241 caagcccgat ttcaacttcg gcggcgtcca gggcggcgcc tcgccctaca aggtcgatca
     6301 agatcccgat cgcatcgcgg tcgaccccta tggccctgag tcccccgaat tcgtcgtcgg
     6361 cgaggacttt atcgatatgt ggtgctcggc gctggcccat tgccagaagc gcttcgaagg
     6421 gcagatgccg ctttacaagc gcgacgagaa ttccggcggc ctcggctgct tcacgccgga
     6481 caatttcccg gtcttcgatg tgtttcgcga gaacgtttat gtcatcgccg attccaatca
     6541 cggctataag atgatcggcg tgggcaagct cgtggcgcag gagctgctcg gcgaaaaaag
     6601 caggctgctc gagccgttcc ggttcgcgcg tttcgccaag ggagaacttc atccgacctc
     6661 tcacagcccg tttccttgga gttgatgaga gcgatatcag tgttacacgg ctggcagtgg
     6721 taagcgggag agtgccatgg atcgacgcgt cgaaacgaag aagagcagta aagcgaaccg
     6781 ctcaaagccc acggatatcg agcaatacgt cgaggcgccg ggacggaagg cactcgtcgc
     6841 cgaggtgcgg aaaaagatcg atgcgctcgg catcgagtat ctctacctcc aattcatctc
     6901 ggtcacgggc cgcatcatgg gcaagggcat cccggccgac cactgggagt cgatcgcgca
     6961 gaagggcttc cagctcgtct acggcgccac catgaacctc ttcctcaacc gccgcggcga
     7021 ctatctcggc tacgggcctg aagccgcgga acttgtcggc atccccgagc ccgagacctt
     7081 catgcagctg ccctgggaca agcgggtggc gcggctctgg tgcacgctgt tccgcaaccg
     7141 cgaggagcgc gaagatccgg gggccttcct caccgccgat tcgcgcggca atcttcgccg
     7201 cctgcacgag cagtttcaga aggaccacgg gctgcagctt cgtcacggca ccgagcccga
     7261 gatgatgtgg ctgaagaagg gcgccgacgg cgagccggcc ggaggctact ccaatcccta
     7321 ttgctatcac atcgatcagt tcgagagctt gcggttggtg tacatgcagg tcatcgacta
     7381 ttgccgcaag atgggcctcg acatgatcca aggcgatcac gaggactcgc ccggacagct
     7441 cgaactcaat ttcaccttcg acgacgcgct cagaaccgcc gaccggctcg ccacctaccg
     7501 ccagatctgc gctcaagtcg cacgcgagtt cggcatcatc gcctgcttca tgtgcaagcc
     7561 attcatgggc gtcagcgcca atggctgcca ccacaacatt tcgctgtggc ggggcggcaa
     7621 ggatgagctg aagtcgctcg gcaacgaccc gaagaagctt cccggcatgg cgcagaacta
     7681 catgtactcc aagggcggcg acaacacctt catgcccgat accgacgacg tccagcttcc
     7741 cggcaagatc gggctgcacg cgatcggcgg catcgtcaag catctcggtg cgctcacggc
     7801 gatcggctgc tcgaccgtca actcctaccg ccggctgtgg gacacgggct tctgggcgcc
     7861 ggtgttctcg gattgggggt tccagaaccg caccacgggc ctacgtgtct cggcccccgg
     7921 ccggttcgaa tatcgcgccg tcgacgccat ggtgaacccg catctgatgg ccgcggcgct
     7981 gatcatggcg atggacgacg gcatcaagaa caagctcgat ccgggtccgt cggaggagcg
     8041 caacatctat caggccatgg aggccggaaa gcaggtgaag aggctgccga tgaccctcgg
     8101 cgacgcgctc gcagcgctcg agaaagaccc ggtgatccgg tcggcgatgc cgggtgaaat
     8161 gtaccggctt tacgacgagt acaagcgcga cgaatgggag cggttcctgc acaccacgac
     8221 ggaatgggat atcaagacct atatggactg cctgccgtga ccgacctcgc caacagagga
     8281 gcgacctgat atgtgcggaa ttgccggcct catccatcgt gacggcgtga ccagcgtcgg
     8341 gcgggagatg acggcgatgc tgaagagcct caagcatcgc ggtcccgact cgaccgggtt
     8401 tgcggtctac ggcgcctcca accccaacga atatgtcctg cgcctcaagc tcgccgagca
     8461 ggaggacatg accaaggatc accacatccg ccgaaagatc agggcgcgcc agtccgaggt
     8521 cgacaagcgc cttgccgagc ttggggtcga gaccctcgaa gtcgcagagg ccacgcccta
     8581 cgccttccgc tatcgcctgc gctatgccgg cgacacgcgg cggctcacga ccgagatcga
     8641 acagatcgaa ggggccgagg tcctctcttt cggctcggcg ctcgagctga tcaaggactt
     8701 gggcgacgcg acccaagtct cggagcaata cgggcttggc accttcaggg gcacgcacgg
     8761 catcgggcat acgcgcatgg cgacggaatc ggacgtcgac attcgctcgg cccatcctta
     8821 ctgggcctat ccctacaacg acatcgccgt ggtccataac gggcagatca ccaattattg
     8881 gatcatgcgg cgggagatgg agcggctcgg acaccgcttc atgtcgaatt gcgattcgga
     8941 gctgctcgcg gtctacaccg caaacaatct cgagaagggt gcaacgctcg aggattcgct
     9001 gaaagcctcg ctcaaggaca tcgacggcgt gttcacctac gtcgtggcga ccgaaaacga
     9061 gctcggcatg gccaaggaca ccatggccgc caagccgatg gtcctgttcg agagcgacaa
     9121 tctcgttgcg ctcgcctccg aggaggtcgc cattcgcgcc atcatgccgc gcgagatcga
     9181 cacgttcgat ccgtatgacg aggagatccg cgtatggcag agatagcgcg gtccgagctc
     9241 agcgaacagg agcgccgctc cttcgagatg gggctgcaca ccgagctgct caagggccgc
     9301 tcgcaccagg tgttcttcga cttcgatctc aaggaagcgc cgcacggctt tgcgctgccc
     9361 aacgcctacg acgtcgattt caacaagcgc gccgagatcg acgccgccga cctcgccacc
     9421 aatgccatca atctgcgcat ccgcgaattg atggacgatg ggtacggcac catcgtcgtc
     9481 cgcaatccgg gcgccaagca ttcgatcgcc gtcggcatcc tgaaccggct caacctcatc
     9541 atcgacgggt cgctcggcta tttcggctgc ggcctgatcg atggaccgaa tgtgcgaatc
     9601 gcggggcgcg tcggctggtc ctgtgccgaa aacatgatga gcgggacgat cctcatcgag
     9661 aagaacgcgg gctcgcagtt cggcgcggcc atccgcggcg gcgatctcgt ctgccgcggc
     9721 aatgtcggct cgcgcaccgg catcgacatg aagggcggca ccatcatcgt cggcggcgat
     9781 accggcgcct tcaccggctt catgatgcag cgcgggcgca tggtgatctg cggcaacgcc
     9841 ggcaagaatc tcggcgactc catgtatgac ggcaccatct tcatcgccgg cgagccgcaa
     9901 agcctcggcg tcgacgcggt ctggggcacc atgaccgagc tcgataagag ctggctcgag
     9961 aacaagctcc gccgctacga gctcttgccg aaggaaggta tcggcgcctt tcgcaagatc
    10021 gtcgccggga agcaattgtg gaactacgac aatctggagc cgacggaaaa gaaactggtg
    10081 ttgtgaggcg gtgagccggg cgggagcgat gaagaagaac agaaacggca agaacggcaa
    10141 agggagtgtc aaggcaccgg ccgaggcgga acggttcatc ctcggcagga gccagatctt
    10201 cacgccggac gtcatcaacg acatccatgt gaagtccgag ctcggtcgct atcgcatgcg
    10261 cggcttctcc ctgttcaaga agatcccgca ttgggacgat ctgactttcc tgcccggcac
    10321 gctgacgcgc ttcgtcatcg aaggctaccg cgagaaatgc gacaccaaga cggtgatcgg
    10381 ccccagggcc aagcggccga tggtgctcga catcccggtc tatgtgaccg gcatgagctt
    10441 tggcgccttg tcttacgagt cgaagatcgc gcttgcgcgc ggcgccacca tggcgggctc
    10501 ggccacctgc tcgggcgaag gcggcatgat ccccggcgag cgccgctact cgatcaaatg
    10561 gttctaccaa tgcatccagt cgcgctacgg gttcaacccg catcacttgc ggcttgccga
    10621 cgcttgcgag ttcttcatcg gacagggctg caaggtcggg ctcggcggcc atttgatggg
    10681 gcagaaggtg accgaccagg tggccgagat gcgctcgctg cctgcgggca tcgatcagcg
    10741 ctcgccggcg cgtcatcccg actggctcgg ccccgacgat cttgcgctca agatcgagga
    10801 ggtgcgggaa gcgaccaatt gggaaatccc gatccagctc aagctcggcg ccgcgcgcgt
    10861 ctatgacgac gtgcgcatgg cggtgaaatg cgggccggac tccatctata tcgacggcat
    10921 ggagggcggc accggcgccg gtccgcatat cgccaccgaa gacacgggcg tccccggcat
    10981 cgccgccatc cgccaggccc gccgcgccat cgacgagctc ggcaagcgcg gcgagatcag
    11041 cctcgtctat gccggaggca tcaggaatgg cggcgacgtg gccaaggcgc tcgcgctcgg
    11101 cgccgacgcg gtggcgatcg gccactcggt gctgatggcg ctcaactgca acaaggaaat
    11161 tccggagtcc gatttcgaga acgagatcgg cgtgccggcc ggctattgct accactgcca
    11221 taccggccgc tgcccggtcg gcgtcgccac gcaggacccg gaattgcgca agcggctcga
    11281 tcccgacgcc gcggccgagc gcgtctacaa tttcctgcat acgctcacgc tcgaagcgca
    11341 gatgctggcg cgcgcctgcg gcaagaccaa cgtccacagc ctcgagccgg aggaccttgc
    11401 ggcgctgacc atggaagcgt cggccatggc gcaggtgccc ttggccggaa ccgactacac
    11461 tgtcggcgtc gagaactatc acaagatcgg atgagcggag ttcgtgcgat gagcggcaag
    11521 agcagcaagg acgccaagaa gtccaaggcg aagaacaacg tcgacaaata tctgaaggat
    11581 ccgagcggga tcgacaccgc gcgcgagaag cagatcggcc agcacatcgg ctatcgctac
    11641 gatgtcaatc ttctcccgga ttacgcccgc ctcacgccct atctgaaaac ctacatcgag
    11701 ttcatgggct gggacgacct caattggctc gaagacgtgc atatgggcta tgaggcgggg
    11761 aagcccgcca tcttcgaccg caacatcaac gggtgggtgc gcattccgaa ggacatgaag
    11821 ctgcccgaca accagcaaga ccgcgacatg ctggcgcgcg aactgctggt gcgctttcag
    11881 acatcgccca accatccgct agtccagctg aacaaggctt atcgcaagtt ctaggcggca
    11941 cctcagtaag tttggcggcg cgaatcctag cgcaatagca ctgacggaag aaaagagaag
    12001 ctttcagcct tcgatggcgg agttggctcg aaggcgtgac taacggggtg aaacaatggt
    12061 tgctcaagct catgctgccc gcgcggcagg cggatcgtcc gaacttcatc gcgtcatcgc
    12121 tcggcgcggg cgccgatgtg tggggcacgg gcacgctcac ggccggcatc gtcgccatcg
    12181 cgctcatccg ttccggtgtt cgtcttccgg cattacgtcc aggatggggg cgtcttcccc
    12241 gcagggatgc tcgcggactt cacgcccacg ggcgggaaga ttggggagcg taaggcggga
    12301 atgttgccct atgtcacgct tgccgccggc atcggcgtcg ccgcgatcgg ctatgtgatg
    12361 ttttggacat gaggctgaag cacgcctgag gccgcggagt ttgcggaaca atccgcccgg
    12421 gcatttcagc gtgtcccggc ggacgtccaa tcgggccgaa attgcagaac ggccacggtg
    12481 tcagggagag ccggattgaa ctgccggtta tgccagatcg cggcctcgcg agccgcttcg
    12541 ccgggctcga gctcgaaaac cgtgcagggc aggcgatagg tctggttgaa ttgcgcgaaa
    12601 tcgaggatgc agcgcccttg cgcgttgagt tgcgccgcga gcggcaagcc ttcgggcaat
    12661 acggcgacga gcttgtcgct gaaattctcg ggctgctgga agtagtcagc ctgcaagaaa
    12721 cgcaaggcac gctcgtaaag ctcttgcgga tcggggaagc gggccgactg gaagcgaaag
    12781 aaggcagtgc tctcggccgg atcctgcggg atcagcagaa ccgtcaaggc tcgggcgatt
    12841 tccctgccgt cgcgatcgag caggcgcggg cgcatgcccg gagagggacg acccccgtct
    12901 tggcgcatgg cgatctgccg caagcggcac tgccaggcga ggaactcgtg cttgatcgtg
    12961 ttggcggccg acggcgtcac ggcgcatggt ctccaggggc gtaaatcaag cgaatccctc
    13021 tcaatcggaa ttgcaaccct ctctggcagc cctcacgccc cggcgaaagg ccagtttttc
    13081 ggccatttaa ctgtgggaaa tcgaatttca tagcgattga cggcccaaag ggcaccgcct
    13141 agcatcgggt tcataagggg gaaaagtctc ggcattagca taggggtgac gctggcgcct
    13201 cagaggcacg tctcgcgcca tcgaccgagg aaaaagcgta aagctgaaat ggtccgctag
    13261 gggccgcacg attgcgtctt aacgcgacgc tttcgacggc attgaggcaa aagggggtat
    13321 atcgcatgga aggcaatctc aatgcactga cgaccatctt cacggaattc tactactggg
    13381 tgacgatcgt tttcatgttt ttcatccatg tcggcttctg cacatatgaa gtcggcgtgt
    13441 cgcgtcggcg caaccatctc aagacgctga tgaagaacgc gatggcgatc ccgctcgtga
    13501 ccgtcacctt cttcttcttc ggctggtgga tctactttgc gtttccgaac ggaccgggca
    13561 ttaccggcgg attgattccg gccccttact cggagccttg gaatccgctg atggcgccgc
    13621 atctctccgg cgtcaaccca aatgaggccg gctcagtgac gcccgccgaa gcgctccttt
    13681 ggcaccgtct caacggcgtg ttctggggcg cgttcctatt gttctcgatc acgaccgcct
    13741 cgatcatctc gggctcgatc atcgagcgcg tccgctcggg cgcgttctgg gtcatcgccg
    13801 tgctgatcgg ctcggtcatg tggatcctcg atgcggcctg gggctggcat ccgttaggct
    13861 ggatggtgaa gctttggggc tatcacgacg cctacgcttc cggcgttgtc catgccatcg
    13921 caggcggcgc tgcgctggcc ttcttgattg tgctcggtcc ccgcatcggc aaattccggc
    13981 cggacgggac gccgcgggat atcccgccgc acaatgagtg gctggtggtg atcggcctgt
    14041 tcctgatcta cacggggttc tggggctttt atgccgcatg caacatcccg atcatctcgc
    14101 cggcgaccat cgccggtcag atcaccggag acacgtggac ggcgacgaac atttatctcg
    14161 ccccgaccac gctgtcggcg atcacgttca acttcctgat gtcgctgtcg ggcggcatga
    14221 tgggtggtta tctattctca aggggcaatc ctttctggac gttctcaggt gggttgtgcg
    14281 gcatcatttc cgcctcggcc ggcaacgatc tctatcatcc gatccaggcg atgctgatcg
    14341 gcgccatcgt gccggtgatc tgcttcaagc tgcattattt cgtcgaacgc cggttcaaga
    14401 tcgacgatgc ggtgggcgcc gtcgccgtgc acggctatgg cggattcctc ggcgtggtcg
    14461 tcgcgggctt catgctgtgg gggcagccgt cctctccgta tgaaggctac gcccacatca
    14521 atccgctcgg caattttgcc ggtgcgtgcc tgatgttcct gctcggcttt gtcccgacct
    14581 atatcgcctg catgatcatg aatgctatgg gactgttgcg cgtgccggcg aaagtcgagc
    14641 tcgagggtgt cgactttgcg cttaaccaca ccttcatgag ttccgtccgc gaagtgggca
    14701 gcgccgagaa ggcgatgatc aaatagatcc gagaggagag ggaaaaatgt cgaccaatgg
    14761 aatgacaagc tgggccgtcg acctcaagga cgtcggcgca atctatccct tccagggaac
    14821 ggaggtgctc ctggtcatcc tcggacttgc cttctggatc ggttggcaca ttctgcagac
    14881 gcgccaggag actgcggaga ttaactccga catggcggcc gaccggagcg gtgacgaaac
    14941 caggtcggcg atcgatcgct attactaaac acgagcaaat ctgcgtttgg gtcgaggcgc
    15001 cggcgtgccg gcgcctcttt cttgcccgcg cggcgcggcc gcttctcatt cgcagaaaat
    15061 ccggtagcgc acgaaatcgt cggccttgcc gaagcgatcg tataggagcc gcgccttcgc
    15121 attcccctcg cgcgtgtgcc aatagatctg cgaccagccg cgctcgcggg caagcgcgat
    15181 caggtcctag atcagcgcgt ggccaaggcc gcggcccctc atgtttggat cgacaaacaa
    15241 gtcctcgaga tagcagcgcg gcgcgagccc ccaggtgccc ggatgcacga ccgagatggt
    15301 gaagccggca acggcgccgt cccattcggc gatccgcgcg aacatgcccg aacccggcat
    15361 gaatagccgc tgccacgttg ccgccgtcac cgcttcgctc accgacgctt cgtaaaaggc
    15421 gacgtagccc gaccaaagaa gacgccaggc ggcctcatca cttggcctcg gatcgcgaat
    15481 aatgggtgcc ggtgcgggca tcccgcgcgc tagcaatcaa gcgtggcggc gcgctcccag
    15541 gccgagagat ggccggcata gtcgttccac tcgcgcatct tcagcttgac gtatgaatcg
    15601 acgaattctt cgccgagctc ccggcgcaac aggtccgatt tttgcgtcat gcgcaaggca
    15661 tcgagcagat tgagcgggag cttcttggtg ccgggcgggg ccttgtgccc ttcggcgtac
    15721 atgtcgatgt cgagcggctt gccgggatcg atgctgcgcg caatgccgtc gaggcccgcg
    15781 gcaagaattg ctgccggaag gaggtaggga ttggcggccc cgtcggcgag ccggaactca
    15841 aaccgtccgc cacccggtat cctgatcatg tgcgtgcggt tgttgccggc ataggtcacg
    15901 gtgtttggcg accaggtggc gcccgacagc gtcaccggcg cgttgatgcg cttatacgaa
    15961 ttgacggtgg ggttggtgat cgcgcagata tcgccggcgg cgttcatcag ccccccgatg
    16021 aaatggtagg cagtgcgcga caggccaagc gcgtccttgt cgtcgtcgaa gacgttcttg
    16081 tttccctgcc acagcgatgc atgcacgtgg cagccgcttc cggtgaggtt ggcgaacggc
    16141 ttcggcatga aggtggcgcg caagccgtgc ttctcggcaa tggccttgac catatatttg
    16201 aagaaggcca tgcggtcggc ggtgacgagc gcgtcgtcga acgcccagtt catctcgaac
    16261 tggccattgg cgtcttcgtg gtcgttctga taggcgcccc agccgagttc gagcatcgca
    16321 tcgcaaatct cgctgatcac gtcgtagcga cgcatcagcg cggactggtc atagcagggc
    16381 ttcacttgcg tgtcggaggc gtcgctgatc gtcgacccgt cggggctcaa gaggaagaac
    16441 tcgcattcca cccccgtctt gagttggaag ccggccttgg ccgccttggc gatcatcttc
    16501 ttgaggatgt tgcgcggggc ctgctcgacc agcttgccgt ccatgtaggg atcggccgca
    16561 agccagccga cctcgggttt ccacgggatt tgaatgaggc ttgcagggtc ggggatggca
    16621 agaacgtcgg ggtgcgcggg cgtcatgtcg agccagctgg caaagccggc aaaacccgcc
    16681 ccgtcctttg ccatgtcggc aatggcggaa gccggcgcaa gcttggcccg gagcgtgccg
    16741 aacaaatcga cataggaaat cagaaaatac tttatcccgc gctcgcgggc cgtggcggcg
    16801 agatcaactg ccatgtgaca ccctttcgct tgacctggct tactccgacg acgaattcgc
    16861 tcccgggatc cagtttgtgc cggcaagcgg cacgcgcgcc atggcggccg cctcgacggt
    16921 gagcgccacg agatcctcgg gctcaagatt gtgaacatga ctcttgccgc aggcccgcga
    16981 aatggtttgc gtctcgagcg tcaagaccga gaggtagttg gcaagccgcc ggcccgcgag
    17041 gaccggatcg agccttgccg caagcgtggg gtcctgggtg gtgattccgg ccggatcgcg
    17101 cccctcgtgc caatcgtcgt agcagccggc ggtggtgccg agcttgcgat attcctcctc
    17161 gagcgcgggg tcgttgtcgc cgagcgcgac cagggcggcg gtgccgatcg ccacagcgtc
    17221 ggcgccgagc gcgagcgcct tggcgacgtc ggcgccgttg cgaatgccgc cggagacgat
    17281 caactgcacc ttgcggtgca tgccgaggtc ctgcagcgca tcgaccgccg gcctgatggc
    17341 accgaggatc ggcagcccga catgctcgat gaacacttcc tgcgtggccg ccgtgccgcc
    17401 ctgcatgccg tcgagcatga tcacatcggc gcccgccttg accgccaatg cggtgtcgta
    17461 gaacgggcgc gaggcgccga ccttgacgta gattggcttc tgccagtcgg tgatctccct
    17521 gatctcctcg atcttgatct cgagatcgtc ggggccggtc cagtccggat gccggcaggc
    17581 cgagcgctgg tcgatgcctt tggggagggt gcgcatctcg gccacgcgat cggaaatctt
    17641 ctgtccgagc agcatgccgc cgccgccggg ctttgccccc tggccgacca cgatctcgat
    17701 ggcgtcggcg cggcgcagat cgtcggggtt catgccgtag cgcgagggga ggagctgata
    17761 gatgagcgtt ttggagtggt ggcgctcctc ttcggtcatg ccgccgtcgc cggtggtggt
    17821 cgaggtgcca agctgggacg cgccgcgccc caaggcctcc ttggcctggg ccgacagcga
    17881 accgaagctc atgccggcga tggtgacggg gatcttgatg tggatcggct tctcggcgaa
    17941 gcgggtgccg agccacacat cggtcgaaca gcgctcgcga tagccctcga gcggataacg
    18001 gctgatcgag gcgccgagaa acaggagatc gtcgaaatgc ggcagcttgc gcttggcgcc
    18061 ggcgccgcgg atatcgtaga tgccggtcgc ggcggcgcgg cggatctccg aaagcgaaga
    18121 cgcatcgaac gtggctgagg ggcgcggggc ggttcgcggg gtccagtcca tcaataggct
    18181 ccggcgtggt cgacgtggaa attgtaga
//
LOCUS       DASUQP010000166         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_901272,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000166 DASUQP010000000
VERSION     DASUQP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901272"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFY74_07305"
     CDS             complement(<1..600)
                     /locus_tag="VFY74_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX5931539.1"
                     /translation="MSAMETPVNEDTQERDALLEWEGGEAELEAGDRLVVDVEGFEGP
                     LDLLLALARTQKVDLAKISVLALAQQYLDFIAEARRLRLEIAADYLVMAAWLAFLKSK
                     LLLPAEPSEEGEPTGAELAALLAFRLKRLDAMREASAQLMTRKRLGRDVFARGLPEPM
                     RVTRKSIYGANVYDLLKAYSQQRQRMAVRSLRMPPRAVWS"
     gene            complement(597..1646)
                     /gene="nagZ"
                     /locus_tag="VFY74_07310"
     CDS             complement(597..1646)
                     /gene="nagZ"
                     /locus_tag="VFY74_07310"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX5931540.1"
                     /translation="MDLKAFISGCAGLALDDAERRFFAATRPCGLILFARNCRTPAQI
                     KTLISDFKDAVGSDEVLVLVDQEGGRVRRLKPPDWRYMPPARCYGRLYATDPAIGTRA
                     AFAGARLTAQELYDLGITVNTMPVLDVPQEGAHDIIGDRAYGTDPETVIPLGRAVIEG
                     CLSGGVLPVIKHVPGHGRATADSHLALPRIDAGLSELEAIDFRPFHALRHAPLAMTAH
                     VLMPAFDDRRPASVSPAIMSEVIRKRIGLTGLIMSDDLGMKALGGSFAERACGVIDAG
                     CDVALHCSGVLAEMAEVADAVPPLEGVGAERFAHARAQLRAPEPFDVAEALALVTEAA
                     GTQVASLGSDPTVPA"
     gene            complement(1711..>2626)
                     /locus_tag="VFY74_07315"
     CDS             complement(1711..>2626)
                     /locus_tag="VFY74_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5931541.1"
                     /translation="AYKQSGGGLGSEPPPLVQADDTPVKEAPDQPGGKEFAHKNKLIY
                     DRLQNGDAPEAEKIVSRQEDVAVPALPAADPAAMPAPVATTDAVPPTTQAVPGAQPVA
                     MGGEDDASMPDGGPRKVKTMVVRPDGSVESPAVPELPTDAAPAAAPPAETAATPAMPA
                     ADPQLAAVSPAPPAAEPQPAQPAPQEAAAPAEPAPKAKPKPAAKPAAQQTAAAAPQAA
                     AAPSKYVVQVGSKKNQTEALASFADMQQKYPTLLASYRPIVQKADLGSKGTWYRLRIG
                     PITDKTAANKLCSQLKSQGLPDCLVMAQ"
BASE COUNT          416 a          878 c          895 g          437 t
ORIGIN      
        1 cgaccacacc gcccgtggcg gcatccgcaa gctgcgaacc gccatgcgct ggcgctgctg
       61 agagtaggcc ttgagcagat cgtagacatt ggcgccgtaa atgctcttgc gggtgacgcg
      121 catcggttca ggcaacccgc gggcgaacac gtcgcggccg agccgtttgc gcgtcatgag
      181 ctgcgccgag gcctcgcgca tggcgtcgag ccgctttagc ctgaaggcga gcagcgcggc
      241 aagctcggcc ccggtcggct ctccctcctc cgacggctcg gcggggagca acagcttcga
      301 cttcagaaag gcaagccatg ccgccatgac cagatagtcg gcggcgatct cgagcctcag
      361 ccgccgtgcc tcggcgataa agtcgagata ttgctgggcg agagcgagca ccgagatctt
      421 ggcgagatcg accttctgcg tccgagcgag cgcaaggagc aggtcgagag gcccctcgaa
      481 accctcgacg tcgaccacga gccggtcgcc cgcctcaagc tccgcctcgc cgccctccca
      541 ctccaagagc gcgtcgcgct cttgcgtgtc ttcgtttacg ggcgtttcca tcgcgctcat
      601 gccggtaccg tcggatcgga cccgagactt gcgacctggg tgccggccgc ttctgtcacc
      661 aacgccaaag cttcggcgac gtcgaacggc tcaggcgcgc gcagttgcgc gcgggcgtgg
      721 gcgaaacgct cggcgccaac cccctcgagc ggcggcaccg catcggcgac ctcggccatc
      781 tcggcaagga cgccgctgca atgaagcgcc acgtcgcagc ccgcatcgat cacgccgcag
      841 gcgcgttccg cgaaggagcc accaagcgcc ttcatgccga gatcgtcgct cattatcaag
      901 cccgtcaatc cgatccgttt tcgaatcact tcgctcatta tagctggcga gacgctcgcc
      961 ggtcgccggt cgtcgaaggc gggcatcagc acatgcgcgg tcatggcgag cggcgcatgg
     1021 cgaagcgcat ggaagggacg gaagtcgatc gcctcgagtt ccgaaaggcc ggcatcgatg
     1081 cgcggcagcg caagatggct gtcggcggtc gcccgcccat ggcccggaac gtgcttgatg
     1141 actgggagca cgccccccga aagacagccc tcgatcacgg cgcggcccaa ggggatcacc
     1201 gtttcggggt cggtgccgta ggcgcggtcg ccgatgatgt cgtgcgctcc ttcttgcggc
     1261 acgtcgagga ccggcatggt gttgacggtg atgccgaggt cgtaaagctc ctgggcggtc
     1321 agtcttgccc ccgcaaacgc tgcccgcgtc cctatcgcgg gatcggtcgc gtacaggcgc
     1381 ccataacagc gcgcgggcgg catataccgc caatccggcg gcttcagccg ccgcacccgg
     1441 ccaccttctt gatcgacgag gaccaggacc tcgtcgcttc cgacggcgtc cttgaagtcg
     1501 ctgatgagcg tcttgatctg cgccggtgtc cggcaattgc gcgcaaacag gatcaggccg
     1561 catggccggg tcgcggcaaa gaagcgacgc tcggcatcgt cgagggctaa gcccgcacag
     1621 ccggagatga aggccttgag gtccacgacg cgggttagcg tgcgccgccg cgcacggtca
     1681 aggccggaag atggcccgaa atggcgctgc ctactgcgcc attaccaggc aatcgggcag
     1741 gccttgcgat ttcaactgac tgcacagctt gttggcggcc gtcttgtcgg tgatcgggcc
     1801 gatgcgcagc cggtaccatg tccccttgga gccaaggtcg gccttctgca cgatgggccg
     1861 gtaactggcg agcaaggtcg gatatttctg ttgcatatcg gcgaagctcg cgagcgcctc
     1921 ggtctgattc ttcttcgagc cgacctgcac gacatatttg gacggagcgg cagcggcctg
     1981 cggtgccgcg gcggcagtct gctgcgcggc aggcttcgcc gcaggctttg gcttggcctt
     2041 cggcgcgggc tccgccggag ccgcggcctc ttgtggcgca ggctgggcgg gttgcggctc
     2101 ggcggcggga ggcgccggcg agacagccgc gagctgagga tcggcggccg gcatagcggg
     2161 ggtcgccgcg gtctcggcag gaggtgcagc ggccggggca gcatcggtgg gaagctcagg
     2221 cacggccggc gactctaccg agccgtcggg acgaacaacc atcgtcttga ccttgcgcgg
     2281 gccgccgtcg ggcatcgagg cgtcgtcttc tccgcccatt gccacgggct gggcaccggg
     2341 gaccgcctga gtcgtcggcg ggaccgcatc ggtggtcgcg accggcgccg gcattgcggc
     2401 gggatcggcc gcaggcagag ccgggaccgc cacgtcctcc tgacgcgaca cgatcttctc
     2461 ggcctccggc gcatcgccgt tctgcaaacg gtcgtaaatc agcttgttct tgtgcgcaaa
     2521 ttccttgccg cccggctgat cgggcgcttc cttgacgggc gtgtcgtcgg cctggaccaa
     2581 gggtggcggc tcgctcccga gtccccctcc gctttgctta taagca
//
LOCUS       DASUQP010000167         9261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_629616,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000167 DASUQP010000000
VERSION     DASUQP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629616"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1884)
                     /locus_tag="VFY74_07320"
     CDS             complement(70..1884)
                     /locus_tag="VFY74_07320"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX5931542.1"
                     /translation="MSVQSKIASEDPKTTNPKVLAFVEEARKLFKPDGVVWCDGSKEE
                     YQRMLKSLVDGGTALWLNPDKRPNSILVRSDPLDVARVEDQTYICSKSKEDAGPTNNW
                     FDPVEMKATLTKLYDGAMAGRTMYVVPYSMGPIGSPIAGIGVMVTDSAYAVANMHIMT
                     RVGDKVWKALGQSEDFVRGMHTVGYPLSTPTTADVPWPCNKTKYISHFPETREIWSYG
                     SGYGGNALLGKKCHALRIASVKARDEGWMAEHMLILKLTNPQGKAKYIAAAFPSACGK
                     TNLAMLNPTIPGWKAETIGDDICWMKFGEDGRLHAINPETGFFGVAPGTSNDSNLNAM
                     KTLRANCIYSNVALTDDGDVWWEQMGTTPEHTVDWMRRSWTPSSGRSAAHPNARFTAP
                     AAQCPVIAKEWEDPKGVPIDAILFGGRRASVVPLVTEAENWQQGTFLGAIMASEKTAA
                     ASGVKGDIRRDPMAMLPFCGYHMGDYFAHWLKMGEKGGAKMPKIFYVNWFRKNGDGKF
                     MWPGYAENSRVLKWIFERCDGTAKAVETPIGKLPAEDAIDVSGLKVPPSDMKELTRMD
                     KEGWKAELPLIKEHFATFGSKLPKALKDELAALEKRLA"
     gene            complement(1994..2752)
                     /locus_tag="VFY74_07325"
     CDS             complement(1994..2752)
                     /locus_tag="VFY74_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEX5931543.1"
                     /translation="MTVQVNKSVEIPDRSEIAQSARSLVRRAFKGSLATIDRSGGYPY
                     ASLITLATDASGAPTFLISTLARHTANLAADVRASVLIDETSALADPLQGARVTLHGR
                     AEQTTGNDDVRRRFLARHPEAAFYADFPDFSFWRLDVEGAHYIGGFGRIFDLEPSELV
                     LPLDGAESLIAAEAEIVEHMNADHADALELYATSLAGASPGGWRMTGIDPEGLDIVLG
                     SEPRRVLFAERASTPETARKELVKLAAEARARRA"
     gene            2871..3566
                     /locus_tag="VFY74_07330"
     CDS             2871..3566
                     /locus_tag="VFY74_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5931544.1"
                     /translation="MPTIALVDDDRNILTSLRMVLEAEGYKTQTYNDGASALDGFAES
                     PPDLAILDIKMPRMDGMELLRRLRQRSELPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FPQRLLVERVKAVLRRAQPRDTGTVVEDKSKVLERGKLKMDPERHTCVWDNRPVTLTV
                     TEFLILQSLAARPGVVKSRDALMDAAYDDQVYVDDRTIDSHIKRLRKKFKAVDSSFDV
                     IETLYGVGYRFKE"
     gene            3576..5396
                     /locus_tag="VFY74_07335"
     CDS             3576..5396
                     /locus_tag="VFY74_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5931545.1"
                     /translation="MTAEADRSWQRPDAASLWKRALRLLRAGRERIGGAFSWLVEHLP
                     FRNGFSSLTRRIVVLNLIGLAILVSGILYLNQFRAGLIDAKVQSLQTQGEIIARAIAA
                     SAGVDTDEVPLDPEKLLEAEQGETGAANDALPNSLEFTIDPERAAPILRPLVKPTGSR
                     ARIYNRDGALILDSDTFYSRGEVLRYDLPPPAAEEPDALTRFWQAVKTRMRQYDLPLY
                     TEIGGANGLAYPEVATALTGTSVPIVRINDKGEMVVSVAVPIQRMRAVLGVLLLSTRG
                     GDIDNIVAAERWGIVRVSLFAAAITIVLSVILANTIAGPVQRLSAAAERVRHSVKARA
                     EIPDFTDRTDEIGHLSGALRDMTNALYKRIDAIESFAADVAHELKNPLTSLRSASETL
                     PLVKSEEAKHRLMEIIQHDVKRLDRLISDISDASRLDAELAREDARPVDMAELVRTTV
                     SLFNDMHRGDKPEVSFEIAYASGAHPYRVTGHDSRLSQVIVNLLDNALSFSPPGGKVT
                     VASRRIGPEIQISVEDEGPGIPDQNLERIFERFYTDRPQESFGQNSGLGLNISRQIVV
                     AHGGRLYAENRPLPGAARGMADPGGCRSGGARFVIRLPAT"
     gene            5421..5888
                     /locus_tag="VFY74_07340"
     CDS             5421..5888
                     /locus_tag="VFY74_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX5931546.1"
                     /translation="MREARELVHGTCVAFGRRGVLLRGEPGAGKSDLALRFLALPPEA
                     EERPALVADDQVWIAAGDKGALMASPPAALAGKIEVRGLGIVEVPFLPEAELKLVCDL
                     VGETDVPRMPPETWERTEIAGIALPALKLAPFEASAPLKLKMALFLAAPADPK"
     gene            5971..6372
                     /locus_tag="VFY74_07345"
     CDS             5971..6372
                     /locus_tag="VFY74_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX5931547.1"
                     /translation="MIGVVIVTHGRLAHEFRAALEHVVGPQEQIETISIGPDDDLDMR
                     RSDMLTALARVDSGNGVVVLTDMFGGSPSNLAISAMDGSNVEVIAGVNLPMLVKLATV
                     RGECRLEDAITQAQEAGRKYISVASQILAAN"
     gene            6385..6687
                     /locus_tag="VFY74_07350"
     CDS             6385..6687
                     /locus_tag="VFY74_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX5931548.1"
                     /translation="MRETRKGPRAGLRFAEVVIRNKKGLHARASAQFVRCAAGFSAKV
                     RVTREGHSVGGTSIMGLMMLCAGQGHTILIEAEGPEARAALEALIALVEKGFGEED"
     gene            complement(6693..7271)
                     /locus_tag="VFY74_07355"
     CDS             complement(6693..7271)
                     /locus_tag="VFY74_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5931549.1"
                     /translation="MRDPHRSATQAAPIYVWDPFVRIFHWTLVVAFTVAYLTEDDLLT
                     LHVWAGYLVGALVVARVVWGFVGSPHARFADFLYAPGTTLRYVRDLMLFRAERHLGHS
                     PGGGYMVILLMVFLTATVITGLVVYGGDQQAGPLAGMFTEDTGEATEEVHEVLANITL
                     ALVIAHIAAVVLASFAHRENLIGAMVTGYKRP"
     gene            7427..8818
                     /gene="ahcY"
                     /locus_tag="VFY74_07360"
     CDS             7427..8818
                     /gene="ahcY"
                     /locus_tag="VFY74_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5931550.1"
                     /translation="MNYHVKDIGLSDWGRKEIAIAETEMPGLMAVRAEYAAKQPLKGA
                     RIAGSLHMTIQTAVLIETLAALGADIRWASCNIYSTQDHAAAAIAAKGIPVFAFKGET
                     LEEYWDFTHRIFEWEDGGTPNMILDDGGDATLLIHLGVRAEGGDTAFLEHATNEEEEV
                     VYAAIKKRLKERPGWYSKIAKNIRGVTEETTTGVHRLYEMQKKGTLLFPAINVNDSVT
                     KSKFDNLYGCRESLVDGIRRGTDVMMAGKVAMVAGFGDVGKGSAASLRNAGCRVLVAE
                     IDPICALQASMEGFEVTTMEDAAPRADIFVTATGNKDVITVDHMRQMKDRAIVCNIGH
                     FDSEIQVAALRNFKWHNVKPQVDEIEFPGGKRIILLSEGRLVNLGNAMGHPSFVMSAS
                     FTNQTLAQIELWTHPGGYERQVYTLPKQLDEKVARLHLGKVGAKLTRLKPDQADYIGV
                     PIEGPYKPEAYRY"
     gene            9063..>9261
                     /locus_tag="VFY74_07365"
     CDS             9063..>9261
                     /locus_tag="VFY74_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931551.1"
                     /translation="MLASVLAALLPFSMLPLEIAMAAGEAAALDARTLLTLGINLGII
                     AFAVGTAIACLRATERARKAEA"
BASE COUNT         1694 a         2988 c         3018 g         1561 t
ORIGIN      
        1 cgtcgccatc caccctcccc tcgcggggag ggattaccca ggcgctcgtc ctccccgcgc
       61 gggggaggat caggcgaggc gcttctccag cgccgcgagc tcgtccttga gcgccttggg
      121 cagcttggaa ccgaaggtgg caaagtgctc cttgatgagc ggcagctccg ccttccagcc
      181 ctccttgtcc atgcgggtca gctccttcat gtcggagggc ggcactttaa gaccgctgac
      241 atcgatcgcg tcctcagccg ggagcttgcc gatcggggtt tcgaccgctt tcgccgtgcc
      301 gtcgcagcgc tcgaagatcc atttcagcac gcgggagttc tcggcatagc ccggccacat
      361 gaacttgccg tcgccgttct tgcggaacca gttgacgtag aagatcttcg gcatcttggc
      421 gccgcccttc tcgcccatct tcagccaatg ggcgaaatag tcgcccatgt ggtagccgca
      481 gaagggcagc atcgccatcg gatcgcggcg gatgtcgcct ttcacgcccg acgcggcggc
      541 cgtcttctcc gacgccatga tggcgccgag gaacgtgccc tgctgccagt tctcggcctc
      601 ggtgacgagc ggcaccacgc ttgcgcggcg gccaccgaac aggatggcgt cgatcggcac
      661 gcccttgggg tcctcccact ccttggcgat caccggacat tgcgcggcgg gcgcggtgaa
      721 gcgggcatta ggatgggcgg cggagcggcc cgatgacggc gtccacgagc gacgcatcca
      781 gtcgacggtg tgctccggcg tcgtgcccat ctgctcccac cacacatcgc cgtcgtcggt
      841 gagcgccacg ttggagtaga tgcagttggc gcgcagcgtc ttcatggcgt tgaggttgga
      901 atcgttggac gtgccgggcg ccaccccgaa gaagcccgtc tccggattga tggcatggag
      961 ccggccatcc tcgccgaact tcatccagca gatgtcgtcg ccgatggtct cggccttcca
     1021 gccgggaatg gtcgggttga gcatggcgag gttggtcttg ccgcatgcgg acgggaaggc
     1081 cgcggcgatg tacttcgcct tgccttgcgg gttggtgagc ttgaggatca gcatgtgctc
     1141 ggccatccag ccctcgtcgc gggccttcac gctcgcgatc cgcagcgcgt ggcacttctt
     1201 gccgagcagc gcattgccgc catagccgga gccgtaggac cagatctcgc gcgtctcggg
     1261 gaaatgggag atgtacttgg tcttgttgca gggccagggc acgtcggcgg tggtcggcgt
     1321 cgacagcgga tagcccacgg tgtgcatgcc gcgcacgaaa tcctcgctct gcccgagcgc
     1381 cttccacacc ttgtcgccga cgcgcgtcat gatgtgcatg ttggcgacgg cgtaggcgct
     1441 atccgtcacc atgacgccga tgccggcaat gggcgagccg atcggcccca tggaatacgg
     1501 caccacgtac atggtgcggc cggccatggc gccgtcatag agcttggtca gcgtcgcctt
     1561 catctcgacg ggatcgaacc agttgttggt cggacccgcg tcttccttgc tcttcgagca
     1621 gatgtaggtc tggtcctcga cgcgggcgac gtcgagcggg tcggagcgca cgaggatcga
     1681 gttcggccgc ttgtcgggat tgagccacag cgccgtgccg ccatcgacca gcgacttcaa
     1741 catgcgctga tattcttcct tggagccgtc gcaccacacc acgccgtccg gcttgaacaa
     1801 tttcctggcc tcttcgacga aggccagcac tttcgggttg gtggtttttg ggtcttcaga
     1861 cgcaatcttc gactggacgc tcatgcggcg ggatcccttg gcgaagcggt gaacgggggc
     1921 ggcaagaccg ctctccccta gcactgatcg cgagggcctt tcaaggcgag ggcgacggca
     1981 aggaaggacc gaatcaggcg cggcgcgcgc gggcctcggc ggcgagcttg accagctcct
     2041 tgcgggccgt ttcgggggtg ctcgcgcgtt cggcgaacaa aacccgccgc ggctcgctgc
     2101 cgagaacgat atcgaggccc tcagggtcga tgccggtcat gcgccagccc cccggcgagg
     2161 cgccggcaag ggaggtggcg tagagctcga gcgcatcggc gtggtccgcg ttcatgtgct
     2221 cgacgatttc cgcctctgcg gcgatgaggc tctcggcccc gtcgagcgga aggacgagct
     2281 ccgagggctc aagatcgaag atgcgcccga agccgccgat atagtgggcg ccctcgacat
     2341 cgagccgcca gaaggagaaa tcggggaagt cggcatagaa ggcagcctcc ggatgccggg
     2401 cgaggaagcg ccgccgcacg tcgtcgttgc ctgtggtctg ctccgcacgg ccgtgaagcg
     2461 tgaccctcgc cccttgcaaa ggatcggcga gagcgctcgt ctcgtcgatc agaaccgagg
     2521 cgcggacgtc agcggcgaga tttgcggtgt ggcgcgcgag ggtggaaatg aggaaggtgg
     2581 gagcgcccga agcatccgtc gcgagcgtaa tgagcgatgc gtaaggatag ccgcccgagc
     2641 ggtctatggt cgccagagac cccttgaagg cacgccgcac gaggctgcgc gcggactgcg
     2701 cgatctcgga tctatcgggg atttccaccg acttattcac ttgcaccgtc acgatatttg
     2761 ttgcaccacg aggtgacggg tggcgaactg cgccccaaag ctgccataat tgaccgcagc
     2821 cctgaacccc tacatcttaa ccaagctaat cgccgcaagg gccctgcaca atgccaacga
     2881 tcgcacttgt cgacgacgac cgtaacattc tcacctcctt gcgcatggtg ctggaggcgg
     2941 agggctacaa gacccaaacc tataacgatg gcgcgagcgc gctggacgga ttcgccgaaa
     3001 gcccgcccga cctcgccatc ctcgatatca agatgccgcg catggacggc atggagctct
     3061 tgcggcggct ccgtcagcgg agcgagctgc cggtcatctt cctcacctcg aaggatgagg
     3121 agatcgacga gctgttcggt ctcaagatgg gcgccgacga cttcatccgc aaaccgtttc
     3181 cgcagcgcct gctggtggaa cgcgtcaagg cggtgctccg gcgcgcccag ccccgcgata
     3241 ccggcacggt ggtcgaggac aagtcgaagg ttctggagcg cggcaagctc aagatggacc
     3301 ccgagcgcca cacctgcgtc tgggacaacc gccccgtgac cttgaccgtc accgaattcc
     3361 tgatcctgca atcgctggcg gcaaggcccg gcgtggtgaa gagccgcgac gcgctcatgg
     3421 atgccgccta tgatgaccag gtctatgtcg acgaccgcac catcgacagc cacatcaagc
     3481 ggttgcgcaa gaagttcaag gccgtcgatt caagcttcga cgtgatcgag acgctttatg
     3541 gcgttggcta ccggttcaaa gagtgacgga cgtcgatgac cgccgaggcg gacaggtcct
     3601 ggcaacggcc tgacgcggct tcgctctgga agcgcgcgct tcggctgctc agggccgggc
     3661 gcgagcgcat cgggggcgcc ttctcctggc tcgttgagca cctccccttc cgcaacggct
     3721 tttccagcct gacgcggcgc atcgtcgtgc tcaacctgat cgggctcgca atcctcgtct
     3781 caggcatcct ttacctgaac cagttccgcg ccgggctgat cgacgccaag gtgcagagcc
     3841 tgcaaacgca gggcgagatc atcgcccgcg cgatcgccgc ctccgccggc gtcgataccg
     3901 acgaagtccc gctcgacccg gaaaagctgc tcgaagcgga acagggtgag accggcgccg
     3961 cgaacgacgc gctgccgaac tcgctcgaat tcaccatcga cccggagcgc gccgccccga
     4021 tcctgcgccc gttggtcaaa ccgaccggca gccgcgcccg catctacaat cgcgacggtg
     4081 cgttgatcct cgattcggac accttctatt cgcgcggcga agtgctgcgc tacgacctgc
     4141 cgccgccggc cgccgaggag cccgacgcgc tcacgcgatt ctggcaggcg gtcaagacgc
     4201 gcatgcggca gtacgacttg ccgctctaca ccgagatcgg cggcgccaat gggctcgcct
     4261 atcccgaggt tgcgacggcg ctcacgggca cgtcggtgcc gatcgtcagg atcaacgaca
     4321 agggcgaaat ggtggtttcg gtggcggtgc cgatccagcg catgcgggcg gtgctgggcg
     4381 tgctgctgct ctccacgcgg ggcggcgaca tcgacaatat cgtcgccgcc gagcgctggg
     4441 ggatcgtgcg ggtgtcgctg tttgccgcgg cgatcacgat tgtcctgtcc gtgatcctcg
     4501 ccaataccat cgccggtccc gtccagcggc tatcggcggc ggccgagcgg gtgcgccaca
     4561 gcgtcaaggc ccgcgcggag atccccgact tcaccgatcg caccgacgag atcggccacc
     4621 tttccggcgc gctccgcgac atgaccaacg cgctctacaa gcggatcgac gccatcgaga
     4681 gctttgccgc cgacgtggcg cacgagctta agaacccgct cacctcgctc aggagcgcgt
     4741 ccgagacgct tccgctcgtc aagagcgagg aggcgaagca ccgcctgatg gagatcatcc
     4801 agcatgacgt gaagcggctc gaccgtctga tcagcgacat ttcggatgcc tcgcgtctcg
     4861 atgcggagct tgcgcgcgag gacgcgagac ccgtcgacat ggccgagttg gtccgcacca
     4921 ccgtctcgct gttcaacgat atgcaccgcg gcgataagcc cgaggtttcc ttcgagatcg
     4981 cttatgcatc gggcgcgcac ccctaccgtg tcacgggcca tgacagccgc ctcagtcagg
     5041 tcatcgtcaa cctgctcgac aacgcgctct cgttctcgcc gccgggcgga aaggtgacgg
     5101 tggcgtcgcg ccggatcggg ccggagatcc agatctcggt cgaggacgaa ggcccgggca
     5161 ttccggacca gaatctcgaa cgcatcttcg agcgcttcta taccgaccgt ccgcaggaaa
     5221 gcttcggtca gaattccgga cttggcctca acatctcccg gcagatcgtg gttgcccatg
     5281 gcggccgcct ctatgccgag aaccgtccgc tcccgggcgc ggcgcgcggg atggccgatc
     5341 ctggcggatg ccgaagcggc ggcgcgcgat tcgtcatcag gctccccgca acctgacctg
     5401 aagcgatacg agaggcatga ttgagagagg cgcgcgagct cgtccatggc acctgcgtgg
     5461 ctttcgggcg gcgcggggtg ctgctgcgcg gggagccggg cgcgggcaag tccgaccttg
     5521 cgctcaggtt tctcgcgctt cctccggaag ccgaggagag gcccgcgctc gtggccgacg
     5581 atcaggtctg gatcgcggcc ggcgacaagg gagcgctgat ggcttcgccg cccgcggccc
     5641 ttgccggcaa gattgaagtg aggggtctcg gcatcgtgga ggtaccgttc cttccggagg
     5701 ccgagcttaa actggtgtgc gatctcgtgg gcgagacgga cgtgccgcgc atgccgcccg
     5761 agacctggga acgaaccgag atcgcgggca tcgcgctccc cgcattgaag ctcgcgccgt
     5821 tcgaggcatc cgcgccccta aagctcaaaa tggcgctctt cctagcggca cccgccgacc
     5881 ccaaataagg caaggatcag acttgcgcat gataccagtc ctgagaaaga tagcgccctt
     5941 tggccgggcg gacctgaagc aaggaacctc atgatcggcg tcgtcatcgt cacgcatggc
     6001 aggctcgcgc acgagttccg tgccgcgctc gagcacgtcg tcggcccgca agagcagatc
     6061 gagacgatct cgatcggacc cgacgacgat ctcgacatgc gccgcagcga catgttgacg
     6121 gcgctcgccc gtgtcgacag cggcaacggc gttgtcgtgc tcaccgacat gttcggaggc
     6181 tcgccgtcca atctcgccat ctcggccatg gatgggtcca atgtcgaggt gatcgccggc
     6241 gtcaatctgc cgatgctggt gaagctcgca accgtcaggg gcgaatgccg gctggaagac
     6301 gccatcaccc aggcgcagga agccgggcgc aaatatatca gcgtggccag ccagatcctt
     6361 gcggcgaact agagcgtggc ccgggtgcgc gaaacgagaa aagggccgcg cgccggcctg
     6421 cgcttcgccg aggtcgtgat ccgcaacaag aaggggctgc atgcgcgcgc gtcggcgcag
     6481 ttcgtgcgtt gcgccgcagg cttttccgcc aaggtccgcg tgacgcgcga aggccacagc
     6541 gtgggcggaa cctcgatcat ggggctgatg atgctctgcg ccggccaggg ccacaccatc
     6601 ctgatcgagg cggaagggcc cgaggcgcgc gccgcgctcg aagcgctcat cgcgctggtc
     6661 gagaagggtt tcggcgaaga ggattgacga tcctaaggac gcttatagcc ggtgaccata
     6721 gcgccaatca ggttctcgcg atgcgcgaag ctcgcaagca ccacggcggc gatatgggcg
     6781 atcacgagag cgagagtgat attggcaagc acctcgtgga cttcttccgt cgcctcgccg
     6841 gtgtcttcgg tgaacatgcc ggcgagcggc cccgcctgct ggtcgccgcc gtagaccaca
     6901 aggcccgtga tgacggtcgc ggttaggaac accatcagga ggatcaccat atagccgccg
     6961 cccgggctat gaccgagatg gcgctcggcg cgaaacagca tcaggtcgcg cacgtagcga
     7021 agcgtggtgc cgggcgcgta gagaaaatcg gcgaaacgcg catgcggcga gccgacaaag
     7081 ccccatacga cccgcgccac caccagggct ccgaccaaat aaccggccca gacatgcagg
     7141 gtgaggaggt cgtcctcggt cagataggcg acggtgaagg cgacgaccaa cgtccagtga
     7201 aagatccgta cgaacggatc ccagacataa atgggggccg cttgggtcgc gctgcgatga
     7261 ggatcgcgca tggctgctta tacatcagcc attgcggcac gtgtttagga gaatcaggac
     7321 ttggcgtgaa ctccctgcca ggtttcatgc ccttgacact tcacttggcg ggtaagatat
     7381 aaggacttct ttatatcttt atcggaacgg aaggcaacgg gccggcatga actaccacgt
     7441 caaggatatc gggctctccg attggggccg caaggaaatc gccattgccg agacggagat
     7501 gccggggctg atggcggtgc gcgccgaata cgcggcgaag cagccgctga aaggcgcacg
     7561 catcgcgggc tcgctgcaca tgacgatcca gacggccgtg ctgatcgaaa cgcttgccgc
     7621 gctcggcgcc gatatccgct gggcctcctg caacatctac tcgacgcaag accacgccgc
     7681 cgcggcgatc gccgcgaagg gcatccccgt cttcgccttt aagggggaga cgctggagga
     7741 atattgggac ttcacccacc gcatcttcga atgggaagat ggcggcacgc ccaacatgat
     7801 cctcgacgac ggcggcgacg ccacgctctt gatccatctc ggcgtccgcg ccgagggcgg
     7861 cgacaccgcc ttcctcgagc acgcgaccaa tgaggaagag gaggtcgtct acgccgccat
     7921 caagaagcgg ctgaaggagc gcccaggctg gtactccaag atcgccaaga acatccgcgg
     7981 cgtgaccgaa gagaccacga ccggcgtgca ccggctctac gagatgcaga agaagggcac
     8041 gctgctgttc cccgccatca acgtcaacga cagcgtcacc aagtcgaaat tcgacaatct
     8101 ctatggctgc cgcgagagcc tggtcgacgg catccgtcgc ggcaccgacg tgatgatggc
     8161 gggtaaggtg gcgatggtcg cgggcttcgg cgacgtcggc aaaggctcgg ccgcgagcct
     8221 tcgcaacgcc ggctgccgcg tgctcgtggc ggagatcgat ccgatctgcg ccctgcaagc
     8281 ctcgatggaa ggtttcgaag tcaccaccat ggaggacgcg gcaccccgcg ccgatatctt
     8341 cgtcaccgcc acgggcaaca aggacgtcat caccgtcgac catatgcgcc agatgaagga
     8401 ccgcgccatc gtctgcaata tcggacattt cgactcggag attcaggtcg cggccttgcg
     8461 caatttcaaa tggcacaacg tcaagccgca ggtggacgag atcgagttcc ccggcggaaa
     8521 gcgcatcatc cttttgtcgg agggaaggct cgtcaatctc ggcaacgcca tgggccatcc
     8581 gagcttcgtg atgagcgcct ccttcaccaa ccagacgctg gcgcagatcg agctgtggac
     8641 ccatccgggc ggatacgaga gacaagtcta caccctgccg aaacagctcg acgagaaggt
     8701 ggcgcgtctc catctcggca aggtcggcgc caaactcacg aggctcaagc ccgaccaggc
     8761 cgactatatc ggcgtgccga tcgaaggtcc ctacaagccc gaggcctacc gctattgaga
     8821 ccgcgcacga gcttgtgaca ggcttaagac gggagcgcga cgcatccggc gcttctgcca
     8881 gtgggcaaga gtcgacaata aagctaagct gtgcctgatt cgccttgagg ctcgcggggt
     8941 caatcggcct gcgttcagtc tgtggcagcg gaagcgaggc gaggccatgg gcgggggcgt
     9001 gggagcgagg gaccatgcgg accagcgggg gtaagcttgc accttcatca aggttgccga
     9061 cggtgcttgc ctccgtgctc gccgccttgc tccccttctc catgcttccc cttgaaatcg
     9121 ccatggccgc gggcgaagcg gcggcgctcg atgcccgcac gctgctcacg ctcggcatca
     9181 atctcggcat catcgccttc gccgtgggga cggccatcgc ctgtctccgc gctacggaac
     9241 gggcgcgcaa ggccgaggcc g
//
LOCUS       DASUQP010000168         3747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_630163,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000168 DASUQP010000000
VERSION     DASUQP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630163"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1083)
                     /locus_tag="VFY74_07370"
     CDS             complement(187..1083)
                     /locus_tag="VFY74_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX5931552.1"
                     /translation="MTGQFLSRLGFAAAILVALSSAANAADKVKAVASFSILGDMVRH
                     VGGNRVEVVTLVGPNADAHVFNPTPADVKALAAADVFFVNGLGFEGWIERLEKSSGFS
                     GEVVVASKGVTPLTMREEEGHRYDPHAWQDLANGKIYVANIRDALVGADPDGESIYEA
                     NAAKYLDAIDAEEAAVKAALGALPEARRKVITSHDAFAYFGKAYGLDIVAPQGVSTES
                     EASAQDVARIIRQIKAAKIPAVFIENITDPRLLDRIARETGAKIGGALYSDALSASGG
                     PAPTYLGMFRHNVGAITAALSS"
     gene            complement(1109..1975)
                     /locus_tag="VFY74_07375"
     CDS             complement(1109..1975)
                     /locus_tag="VFY74_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5931553.1"
                     /translation="MIDAVIAPFVEFSFMRRALVGCLALSLGAPPIGVFLMLRRMSLM
                     GDAMSHAVLPGAAIGYLLFGLSLPAMSLGGFVAGLLVALAAGLVARSTMLREDASLAG
                     FYLVSLAAGVLIVSIGGSNIDLLHVLFGTVLALDDAALVLVATIATVSLIVLAVIYRP
                     LVLECFDPQFLRSASGWSSPTHLIFLVLVVLNLVAGFQALGTLMAVGIMLLPAITARF
                     WAEDVSGLILVAIGCAVAASLFGLLLSYYANLPTGPSIILLCGVFYLLSMLLGVRGGF
                     IWNLVPRQHLEA"
     gene            complement(1972..2745)
                     /gene="aztA"
                     /locus_tag="VFY74_07380"
     CDS             complement(1972..2745)
                     /gene="aztA"
                     /locus_tag="VFY74_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HEX5931554.1"
                     /translation="MAKREPAISFRDVTLGYDRHPAVHHITGEVESGALLAVIGPNGA
                     GKSTLLKGIVGELRPLGGSIKINRVKGSGIAYLPQQIEIDRFFPISVFDCVSMGLWRE
                     IGAWRGLDRHYRNEVTRALATVGLQDLSDRLVGALSGGQFQRVLFARLLLQDASIILL
                     DEPFRAVDTKTVADLIALIERWHKEGRTVLAALHDLEQVRAHFPDTLLLAREVVAWGE
                     TRKVLTSANLAKSRQLIEAFDEHAEICERDEAARARQSA"
     gene            complement(2748..2945)
                     /locus_tag="VFY74_07385"
     CDS             complement(2748..2945)
                     /locus_tag="VFY74_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931555.1"
                     /translation="MGSDRRGEPHLHVTLVATDRAATSMHRPSAAGAGFSLLRLSLAE
                     RLIIAAAASGLLWGAVFWALR"
     gene            complement(2974..3378)
                     /locus_tag="VFY74_07390"
     CDS             complement(2974..3378)
                     /locus_tag="VFY74_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5931556.1"
                     /translation="MNAAGHHHHPKLSRNQSEILTCLRQAREPMSAYAILDRVRTAGI
                     SHPPTVYRALNELMQKGMVHRLQSRAAFIACDHGACDGKLAFAICRQCGKVVEIPLSD
                     ADEAALLGLAPDQIRPDRVTVEIAGLCEACRA"
     gene            3544..>3747
                     /locus_tag="VFY74_07395"
     CDS             3544..>3747
                     /locus_tag="VFY74_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931557.1"
                     /translation="MLRIALSLLVLGFAGSVALAADEAQPPASEGAPAAQPPKEGAGA
                     ATAGKPLETARTAEKGSLESPYAG"
BASE COUNT          733 a         1212 c         1210 g          592 t
ORIGIN      
        1 cgggaccatg gccgctcgta cgccaaaggc tagaacatta tcccatcgaa ttgaatcatg
       61 acccccctct tactccccct ttcaggggtg agtaaaattg gagcggcgta ttgctctccc
      121 tccccctgca agggggaggg ccgaggtggg ggtcggcagc gtggttcaat ccgacgaaat
      181 aatgcgctag gacgacagcg cggcggtgat cgccccgaca ttgtggcgga acatgccgag
      241 ataggtcggc gccgggcccc cggacgcaga tagcgcatcg gaatagagcg cgccgccgat
      301 ctttgcgccg gtctcgcgcg cgatccgatc cagcaggcgg gggtcggtga tgttttcgat
      361 gaacaccgcg gggatcttcg cggccttgat ctgacggatg atcctggcca cgtcctgcgc
      421 cgacgcctcg ctctcggtgc tcacaccttg cggcgcaacg atatcaagcc cgtaagcctt
      481 gccgaaataa gcgaaggcgt cgtgcgaggt gatgaccttg cggcgcgcct ctggcagtgc
      541 cccgagtgcc gccttcaccg ccgcctcctc cgcatcgatg gcgtcgagat atttggcggc
      601 gttcgcttcg tagatcgact cgccgtccgg atcggcgcca accagagcgt ccctgatatt
      661 ggcgacatag attttgccgt tggcgaggtc ctgccaggca tgagggtcat agcgatggcc
      721 ttcttcctcc cgcatcgtga gcggcgtcac gcccttgctc gccaccacca cctcgccact
      781 gaagcccgaa gatttctcca gccgctcgat ccatccctcg aagccgaggc cgtttacgaa
      841 gaacacgtcc gcggcggcaa gggccttgac gtccgcgggc gtcgggttga acacgtgagc
      901 gtcggcgttt ggcccgacca gcgtcaccac ctcgacccga ttgccgccga cgtgcctgac
      961 catgtcgccg aggatgctga acgaagcgac ggccttgact ttgtcggcgg cgttcgccgc
     1021 gctcgacaaa gctacaagaa ttgcggccgc aaatccgagc cgcgagagga actggcctgt
     1081 catcacgcaa acgcctcccg ctctaggatc aggcttcgag atgctggcgc ggaacgagat
     1141 tccagatgaa accgccacga acgccgagca gcatggaaag gaggtagaag acgccgcaaa
     1201 gcaaaatgat cgacggcccg gtcggcagat tggcgtaata agagaggagc aggccgaaga
     1261 gactcgccgc gacggcgcag ccgatcgcca ccaggatcag ccccgataca tcttcggccc
     1321 agaaccgcgc ggtgatcgcc ggaagcagca tgatgccgac cgccatcagc gtgccgaggg
     1381 cctggaaccc cgccaccaga ttgagcacca cgagcaccag gaagataaga tgggtgggcg
     1441 agctccagcc gctcgcggag cgcaggaact gcggatcgaa gcattcgagc acgagcggcc
     1501 ggtagatgac cgcaagcacg atgagcgaga cggtggcgat cgttgcgaca aggacaagtg
     1561 ctgcgtcgtc gagcgcgagc acagtcccga acagcacatg caggaggtcg atattgctgc
     1621 ccccgatcga gacgatcagc acgcccgccg cgagcgacac gagatagaag ccggcgaggc
     1681 tcgcgtcctc gcgcaacatc gtcgaacggg cgacgaggcc ggcggcgagc gcgaccaaaa
     1741 gcccggcgac gaagcccccg agcgacatgg ccgggagcga caggccgaac agaagatagc
     1801 cgatcgctgc gcccggcagc acggcgtggc tcatggcgtc gcccatcagg ctcatgcgcc
     1861 gcagcatgag gaacacgccg atcggcggag cgccgagcga cagcgccaag cagccgacga
     1921 gcgcacggcg catgaaggaa aattcgacga acggagcgat gacggcgtcg atcatgccga
     1981 ttgccgcgcg cgtgcggcct cgtcccgctc gcagatctcg gcgtgctcgt cgaaggcctc
     2041 gatcagctgg cgcgacttgg cgagatttgc cgacgtcagg actttgcgcg tctcgcccca
     2101 ggccacgact tcgcgcgcga gaagaagcgt gtccgggaag tgcgcccgca cctgctcgag
     2161 atcatgcagc gcggcgagca cggtgcgccc ttccttatgc cagcgctcga tcagcgcgat
     2221 caggtcggcg acggtcttgg tatcgacggc gcggaacggc tcgtcgagca agatgatgct
     2281 ggcgtcctgt agcagaagcc gcgcgaacag cacccgctgg aactggccgc ccgagagcgc
     2341 gccgacgaga cgatcggaga ggtcttgcaa tccgaccgtt gcgagcgcgc gcgtgacctc
     2401 attcctgtaa tgccggtcga gtccgcgcca cgcaccgatc tcgcgccaca gccccatgga
     2461 aacgcagtcg aacaccgaga tgggaaagaa gcgatcgatc tcgatctgct gcgggaggta
     2521 ggcgatcccg gaccccttca ctctattgat cttgatgctg ccgccgagcg gccgaagctc
     2581 gccgacgatg cccttgagca gggtcgactt gccggcgccg ttaggcccga tgacggcgag
     2641 aagcgccccg gactcaacct ctcccgtgat gtgatggacg gccggatgcc ggtcgtagcc
     2701 aagcgtcacg tcgcggaagg aaatcgccgg ctctctcttc gccatgctca cctcaaggcc
     2761 caaaacacgg cgccccaaag cagccccgag gcggcggcgg cgatgatgag ccgctcagcg
     2821 agcgagaggc gcaacagcga gaagccggcg cccgcggcgc tcggcctgtg catcgaagtc
     2881 gcggcgcgat ccgtcgccac cagcgtcaca tgaaggtgag gctcgcctcg ccgatccgaa
     2941 cccattgtcg atcctcttgt cgtgacgtca tgcctaggcg cggcaggctt cgcacagacc
     3001 cgcgatctcg accgtgaccc gatcgggtct gatttggtcc ggcgcgagac cgagcagagc
     3061 agcctcgtcc gcatcagaga gaggaatctc gaccaccttc ccgcattggc ggcagatggc
     3121 gaaggccaac ttgccgtcgc aggcgccgtg atcgcaggcg atgaaggccg cgcgcgactg
     3181 aagccgatgc accatgccct tctgcatcag ctcgttgagc gcgcggtaga cggtcggcgg
     3241 gtgggagatc ccagcggttc tgacccggtc gaggatcgcg taggcgctca tcggctctcg
     3301 ggcctgtcgc aggcaggtga ggatctcgct ctggtttcgc gacagcttgg ggtggtggtg
     3361 atgtccggcc gcgttcattg actatgatat aacataacat tattggcttt cagtcaagcg
     3421 gttcctgcgc ttctcgaaaa tgcgtcgcca accggcattg cgacggccgg aacctaaggc
     3481 ggaaagcgcc gtttgccctt tgaaagtgcc gcctccctgg ccgcaaattt cgaggatatg
     3541 cccatgctta gaatcgcact gagtctcttg gttctcggtt ttgcgggcag cgttgcgctc
     3601 gctgccgacg aagcccagcc gccagcgtcg gaaggtgccc ctgccgcaca gccccccaag
     3661 gaaggtgccg gagctgcaac cgccggcaag ccgctcgaaa ccgcgcgcac ggccgagaag
     3721 ggcagcctcg agagccccta tgcgggt
//
LOCUS       DASUQP010000169        11033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1855494, whole genome shotgun sequence.
ACCESSION   DASUQP010000169 DASUQP010000000
VERSION     DASUQP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11033
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855494"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFY74_07400"
     CDS             complement(<1..713)
                     /locus_tag="VFY74_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5931558.1"
                     /translation="MSMISVIGWDLGGANLKLARIEGRRAVQVTLIPCPLRQDPSKFD
                     AAIAEALPLCPTGAVHAVTMTGELSDVFSDRASGVAYLVEMMRKITDGEAIFYGGRAG
                     FLHDIGAVEHTIDVASANWHASASLIARIVPDALLIDAGTTTTDLVPVRHGTVAAKGY
                     SDGERLAEGELVYTGVVRTPVMAVARTAPFRGRMQGVAAERFATMADVWRLLGALPAG
                     ADPYATPDLKGKSTQESAS"
     gene            805..1536
                     /locus_tag="VFY74_07405"
     CDS             805..1536
                     /locus_tag="VFY74_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX5931559.1"
                     /translation="MHEANDFTIIPTLDLKDGIVVHAKAGDRAAYRPLASPFGNADDP
                     ASIARGLLAATGSSVLYIADLDAITGSGSNFELVRGLGYALPDATIWVDAGFSKFADC
                     AFWLPLGATLVIGSESLSSGDDWLEIKEAFEASVVLSLDSDDRGPRGLETIFADPASW
                     PDRVIAMNLARVGTAQGPDIEGLKSLIARAQDRAVFAAGGVRNARDLAAIEAAGARGA
                     LVATALHDDALTQKEIAALLRERRP"
     gene            complement(1643..2152)
                     /gene="fae"
                     /locus_tag="VFY74_07410"
     CDS             complement(1643..2152)
                     /gene="fae"
                     /locus_tag="VFY74_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HEX5931560.1"
                     /translation="MPKISGVCVGESLVGDGNEVAHVDLLLGPRGSAVETAFCNALTN
                     NKDGFTTLLAVVAPNLLTKPPTILYNKVTIKDARQAVQMFGPAQYGVAKAVADSVAEG
                     VIPANEADDLYVTVGVFIHWEAKDDKKIQDFNYRATKEAIARAVKGEPKASEVTAKKD
                     AAHHPFAAG"
     gene            complement(2357..3208)
                     /locus_tag="VFY74_07415"
     CDS             complement(2357..3208)
                     /locus_tag="VFY74_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HEX5931561.1"
                     /translation="MTGPLNPEAIARAFLDACLAELDALKPGNVHRFSEGDRMTVADF
                     AASAEAAAPLIGREGLGVGERIRLAVEATRRAVRQNTNLGIILLAAPLAAAALDRRGG
                     GLAKKLHLILNKLTVDDARQAYRGIRETKPGGLGEAPEHDVAAEPAVTLLQAMRAAEG
                     RDRIAWNYAHDFADVFALGRKWLIQGRERWGDGPWAVTRVYLGFLGHLPDTLIERKFG
                     ARSAAMVREEAAPIEASFIESQAPDTMIAPLMAFDGSLKERGLNPGTSADLTVATLFA
                     SMLEPPN"
     gene            complement(3205..4140)
                     /locus_tag="VFY74_07420"
     CDS             complement(3205..4140)
                     /locus_tag="VFY74_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEX5931562.1"
                     /translation="MGTQRGNDAAIVMFGDGRDWHGRSLARAFKRRGITPLIAPLNAC
                     GFSTETATGLFIPGLGDRLPEGAFVRFVPGGSFEAVTLFLGVLHALRELGVTVWNDAR
                     AIERCVDKSTTTFFLERAGLPTPRTFARANREEAEAITSAVLAEGHKLVQKPLFGAQG
                     KGLRLISSPADLASPEEVNGVYYLQQFITPAQQHHRDWRLFVCRGRIIAAMIRHGSTW
                     ITNIKQGARAEAAIASQELADLAVRAASCVGADYAGVDIIQAADGRFLVLEVNSMPAW
                     NGLQRVSRVRISDRLVDAFLDAALAPQRREGQEGA"
     gene            complement(4145..5149)
                     /gene="mch"
                     /locus_tag="VFY74_07425"
     CDS             complement(4145..5149)
                     /gene="mch"
                     /locus_tag="VFY74_07425"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HEX5931563.1"
                     /translation="MNDSERRAASRSAARAWPSVNVEAEPLVKALIADADALRVDVTE
                     GTLGECRIDCGVNAVGGLEAGRRIGEICLGGLGSVSLATTEPDSTWPFMVTVHTSQPV
                     LACLGSQYAGWSLSAEVKGKKYSVLGSGPARAIGSSEKLFDELGYRDTAASAVLVLEA
                     DRPPPPALVEMVAKACKLKPNRLTFIYAPTSSLAGTVQIAARCLEVALHKAHELHFPL
                     DHIVDGIATAPLPPPAPDFIAAMGRTNDAIIYGGRAQLFVSSGAKEARSLAENLPSLK
                     SKDYGKPFAAIFAAVKGDFYAIDRMLFSPAMVTVTSLKTGESFEGGRIDTAVLSRSFA
                     "
     gene            complement(5049..6296)
                     /locus_tag="VFY74_07430"
     CDS             complement(5049..6296)
                     /locus_tag="VFY74_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5931564.1"
                     /translation="MPRNSPREGGSLLIASISGRALAGAARRAGFTPLVADFFADADT
                     QEAAHACRKLPGSIAFGFRWQTLYPALQTLAALAPSPVLGLVYGSGFEDRPEVLRLIA
                     KRWPLLGTDAATVARLKSPEQFFPALDRLGIAHPPTSSEPRARGAWLAKKRGGAGGSH
                     IVPGRRAKAAANTYFQERVAGRAVSALFIANGSGARVLGFSEQWTAPRPASPWRYGGA
                     VGPVAISAPVARQMTAAVVKVARTYAIKGMASADFVVRDEDPLLLEINPRPGATLDIF
                     DRGARPLLRLHLEAVGQGKLPARVSKFQDAMASAIVYATRGSAAPAVTTWPAWVADRP
                     KPSEWIDKNRPICTVLARAGTAAGAKRLVEARRRRILKLVQGISRSDAGERQRETSRF
                     TKRSKSLAERQRRGGASRQGAHR"
     gene            complement(6274..7170)
                     /locus_tag="VFY74_07435"
     CDS             complement(6274..7170)
                     /locus_tag="VFY74_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HEX5931565.1"
                     /translation="MGNPRILHMITPLKHMSPFDVNMALDAGFDAAIPYTNVTVEDVA
                     GLVQDAIFSRGPEGVKRTGVFIGGKRAMEALDMMDRAKKAMVPPFEISVFADPAGSFT
                     TAAAMVACAKEALKEKFSAGLKGKRVVVFGGTGVVAFASAVIAAMDGASSILVGYDGP
                     ERVRRLADEARARFGVDVSFADGSTEEQKAALARDADIIFSAGPAGRRLLTVDQLKEA
                     KNLLVAADVNAVPPAGVEGVGVNDDAKPIKGTKAVGIGALAVGNIKYRTEAGLFKQMI
                     ESEKPLYLDFRDAYALAQKLAA"
     gene            complement(7316..8323)
                     /locus_tag="VFY74_07440"
     CDS             complement(7316..8323)
                     /locus_tag="VFY74_07440"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX5931566.1"
                     /translation="MEAFPREPRHASPAPARPREAIVTATARLHFGFLDPSGRSARPF
                     GSFGLSLDQPRTRLVLRRAEKSATSGPERERALRYLNEIAASCGIEQTYALRIEEAIP
                     PHAGLGSGTQLALAVGSAFGALEGLRLEPQEVAARLGRGARSGIGIATFAQGGAVLDG
                     GPLDGNLPKLLSRVPFPSSWRVLLIFDEAAKGLVGANEIAAFQTLPDFPPREIEALSQ
                     AITQGALPALAAGDFAAFCRHVGDLQARMGAYFAPSQGGAYASPRVAEVLAWLRDQGI
                     VGLGQSSWGPTGFAFAAAQAEGEALLDRLRARCKHPGLSFALAQGRNEGARIEISEDV
                     N"
     gene            8396..8782
                     /locus_tag="VFY74_07445"
     CDS             8396..8782
                     /locus_tag="VFY74_07445"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5931567.1"
                     /translation="MATAERTYSNAEVEERLKKELPHWYLEDGWIRRRYRTNSWKGTL
                     MVINAVGHLSEAAWHHPDITASYAWVEVRLMNHAAKGITDKDFELAKRIEDLVQWQPG
                     KEGGALEGTPAEDMRFAYIKYDKPKA"
     gene            8915..9823
                     /gene="fhcD"
                     /locus_tag="VFY74_07450"
     CDS             8915..9823
                     /gene="fhcD"
                     /locus_tag="VFY74_07450"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HEX5931568.1"
                     /translation="MTELKIKGVTIVETFAEAFPMVGTRIVITAPSLEWALTAARTMT
                     GFATSVIACGAEAGIERTLSPDETPDGRPGVAVLLFTMDTEKLQRQLRNRVGQCVLTS
                     PGSACFAGLDGEKKLKLGASLRFFGDGWQMSKLMGGRRFWRIPVMDGEFLCEATTGLT
                     KKAVGGGNLLVLGKSHGAVLAACERAVKAIAEIEGAITPFPGGIVRSGSKVGSKYKGQ
                     IASTNDAYCPTLKAAVDTALPLEVEAVLEIVIDGLTKDSVAKAMRAGCHAIAEGGADT
                     GVTHITAGNYGGNLGPHHFHLKEIVA"
     gene            9820..10623
                     /locus_tag="VFY74_07455"
     CDS             9820..10623
                     /locus_tag="VFY74_07455"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HEX5931569.1"
                     /translation="MSALIFELRSAPDERLDLAPLTPERLDGLKSKEIEALAIGTTRV
                     PLTVGDAFKLKGNDPAELRFVGTDMRCDKIGAGMTKGAIIVDGDAGAYLGAGMKKGTI
                     EVNGNAGVLAGASMAGGTIAISGDAGERGGGINVGETLGMRGGVLTIGGKAGSLLGER
                     MRRGLVVVGGDAGDYAGGRMVAGTILFKRSVGANAGFGLRRGSLIFVEEPKDVLPTFG
                     DCGVLEFDYLRLLENWLRDNGKPVNLGSRARRLMGDMSVLGKGEMLILA"
     gene            10690..>11033
                     /locus_tag="VFY74_07460"
     CDS             10690..>11033
                     /locus_tag="VFY74_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase, component I"
                     /protein_id="HEX5931570.1"
                     /translation="MTGSPYVLLDDSLTPGGRSLLYTGAEHVVAAYTPAEVEQALEQV
                     EAGLARGLHAAGFFAYELGYCLEPKLRHLIPDGRRVPLFWLGLFRAPLPLDDPGTRAW
                     LGTHAAAGQATIS"
BASE COUNT         1879 a         3651 c         3659 g         1844 t
ORIGIN      
        1 ctgcttgcgc tttcttgcgt gctttttccc ttgagatcgg gcgtggcgta gggatcggcg
       61 cccgcgggaa gcgcgccaag caggcgccaa acatcggcca tcgtcgcgaa gcgttccgcg
      121 gcgacgccct gcatgcgacc cctgaacggc gcggtccgcg ccaccgccat caccggcgtg
      181 cgcacgaccc cggtatagac gagctcgccc tctgcgagcc gttcgccgtc ggaatagcct
      241 ttcgccgcga ccgtgccgtg cctgaccgga acaaggtccg ttgtcgtggt gccggcatcg
      301 atgagcaaag catcgggaac gatccgggcg atcaggctcg cgctcgcatg ccaattggcc
      361 gatgccacgt cgatcgtgtg ctcgaccgcg ccgatgtcgt ggagaaaacc cgctctcccg
      421 ccgtagaaga tagcttcgcc atcggtgatc ttccgcatca tctcgacgag ataggccacg
      481 ccgctcgctc gatctgagaa cacgtcggag agctcgcccg tcatggtgac ggcatgcacc
      541 gcgcctgtcg gacagagcgg aagcgcttcg gcaatcgccg catcgaactt gctcggatct
      601 tgccggagcg ggcaggggat cagcgtgacc tgcaccgcgc gccgtccctc gatgcgggca
      661 agcttgaggt ttgcgccgcc gaggtcccag ccgatcaccg aaatcatgct catactgcct
      721 cgcgctacgc ctgaccccta tgaggagcgg cttgcccggt caagctgggc gatccgcgtg
      781 gcttcctgct atagataacg ggcaatgcac gaagcgaacg atttcacgat cattccgacg
      841 ctcgacctca aggacggcat cgtcgttcat gccaaagcgg gggatcgcgc cgcgtatcgt
      901 ccgctcgcaa gccccttcgg caacgccgac gatcccgcga gcatcgcccg cggtctgctc
      961 gccgccaccg gctcgtccgt tctctatatc gccgatctcg atgcgatcac aggcagcggc
     1021 tcgaatttcg agctcgtgcg cggcctcggc tatgcgctac ccgacgccac gatctgggtc
     1081 gatgccggtt tctcgaagtt cgccgattgc gccttttggc tgccgcttgg cgcgacgctc
     1141 gtgatcggga gcgagagcct tagttcgggc gatgattggc ttgagatcaa agaggccttc
     1201 gaggcgagtg tcgtgctgtc actcgattcc gatgaccgcg gaccgagagg cctcgaaacg
     1261 atcttcgccg atcccgcgtc atggccggac cgcgtcatcg ccatgaacct cgcccgcgtc
     1321 ggcactgccc agggccccga catcgagggg ctcaagtctc tcatcgccag ggcccaagac
     1381 cgcgcggtct ttgcggcagg cggcgtcagg aacgcgcggg acctcgccgc catcgaagcg
     1441 gccggcgccc gcggcgcgct cgtggcgacg gcgttgcacg acgatgcgct gacgcaaaaa
     1501 gagatcgccg ccctcttgcg agagcggcga ccttaggcgc caaaggcgcc catcttcaga
     1561 aacccagctt ttggagccac cgccgaatcg ggcccggtcc agtcgatgtg ccgggttgcc
     1621 cccgacctgg gggttctccc tttcaaccgg ccgcgaacgg atggtgcgcg gcgtctttct
     1681 tcgcggtgac ctccgacgcc ttcggctcgc ccttgacggc gcgggcgatg gcttccttgg
     1741 tagcgcgata gttgaagtcc tggatcttct tgtcgtcctt ggcttcccag tgaatgaaca
     1801 cgccgacggt gacatacaga tcgtcggcct cgttggcggg gatcacgcct tcggcgacgg
     1861 agtcggccac ggccttggcg acgccatatt gtgccgggcc gaacatctgc accgcctggc
     1921 gcgcatcctt gatggtgacc ttgttgtaaa ggatcgtcgg gggcttggtg agaaggttcg
     1981 gcgcgaccac ggcaagcagg gtggtgaagc cgtccttgtt gttggtcaag gcattgcaga
     2041 acgccgtctc gacggcgctg ccgcgcggac cgagaagcaa atcgacatga gcgacctcgt
     2101 tgccgtcacc gacaagcgac tcgccgacac atactccact gatcttgggc atgaaccccc
     2161 tcctaatggg ctgatatgaa gcaaatcaac taagcgggcg cctgcggcaa tgccgccgat
     2221 cgcaagccga cgagagtcgt ttggaccaag caccttcgtc cgcaactcca atgagaagct
     2281 cggcggcgtg aacataagcc gggggcggaa ccatgggcaa gtcgattatt ggcggccgcg
     2341 cccttgattt ccggcctcaa ttcggcggtt cgagcatcga tgcgaacagc gtcgccacgg
     2401 tcaggtcggc cgaagtgccg gggttcagcc cgcgctcttt cagcgacccg tcgaacgcca
     2461 tcaggggcgc gatcatggtg tccggcgcct ggctttcgat gaagctcgcc tcgatcggag
     2521 ccgcctcctc acgcaccatt gccgcactcc gcgcgccgaa tttccgctcg atcagcgtat
     2581 cggggaggtg gccgagaaag ccgagataga cacgcgtcac ggcccaaggc ccgtcgcccc
     2641 agcgctcgcg gccctgtatc aaccatttgc ggccgagcgc gaaaacatcg gcgaagtcat
     2701 gggcgtaatt ccaggcgatg cggtcgcgtc cctcagccgc gcgcatcgcc tggagcaagg
     2761 tcaccgcggg ctcggcggcg acgtcgtgct cgggggcttc gccgagaccg ccgggcttcg
     2821 tctcccggat gccgcggtag gcttggcggg cgtcgtcgac ggtgagcttg ttgaggatga
     2881 ggtgaagttt tttcgcgagg ccgcccccgc gccggtcgag agccgccgcc gcaaggggcg
     2941 ccgcgaggag gatgatgccg agattggtat tctgtcggac cgcgcgccgc gtcgcctcga
     3001 ccgcgagccg aatgcgctca ccaacaccca gtccttcgcg gccgatgagg ggtgctgccg
     3061 cttcggcgct tgccgcgaaa tcggcgacgg tcatgcggtc gccctcgctg aagcgatgca
     3121 cgtttccggg cttcagcgcg tcgagttcgg caaggcaagc gtcgaggaag gcccgcgcga
     3181 tcgcttcggg gttgagcggc ccggtcaagc gccctcttgg ccttcgcggc gctgaggcgc
     3241 gagcgcggcg tcgaggaaag catcgaccag ccggtcggag atgcgcacgc ggctcacgcg
     3301 ctgaagtccg ttccacgccg gcatcgagtt gacttcgagc accaggaacc gtccgtcggc
     3361 ggcctggatg atatcgacgc cggcgtagtc ggcgccgacg caagacgcgg cccttaccgc
     3421 gagatcggca agctcctggc ttgcgatcgc ggcctccgcg cgcgcccctt gcttgatgtt
     3481 ggtgatccag gtcgacccgt gtctgatcat ggcggcgata atgcgccctc tgcatacgaa
     3541 gaggcgccaa tcccgatggt gctgctgcgc cggcgttatg aactgctgga gataatagac
     3601 cccgttgacc tcctcgggcg aggcgagatc ggcgggggac gaaatcaggc gcaagccctt
     3661 gccttgggcg ccgaacagcg gcttctgcac caatttgtgt ccctcggcga ggacggccga
     3721 ggtaatcgcc tcggcctctt cacggttcgc gcgcgcgaag gtgcggggcg tcggaaggcc
     3781 ggcgcgctcg aggaagaagg tcgtggtcga cttgtcgacg cagcgttcga tggcgcgggc
     3841 gtcgttccac accgtcacgc cgagctcgcg gagcgcatgc agcacgccga gaaagagcgt
     3901 gaccgcctcg aaactgccgc cgggaacgaa gcggacgaac gcgccttcgg gcagacgatc
     3961 gccgagaccc gggatgaaga gcccggtcgc cgtctcggtc gagaagccgc aggcgttgag
     4021 cggggcgatg agcggcgtga tgccgcgtcg cttgaaggcg cgggcgaggc ttcgtccatg
     4081 ccagtcgcgg ccatcgccga acatcacgat ggcggcgtcg ttgcctctct gtgtgcccat
     4141 ggtgttaggc gaaggagcgc gacagaacgg ccgtgtcgat gcgcccgccc tcgaaactct
     4201 cgccggtctt gagcgaggtc accgtcacca tcgccgggct gaacagcatg cggtcgatcg
     4261 cgtagaagtc gcctttcacc gcggcgaaga tggcggcgaa gggtttgccg taatccttcg
     4321 acttgaggct cggcaaattc tcggcaagcg acctggcctc cttggcgccg ctcgatacga
     4381 agagctgggc gcggccgcca tagatgatgg cgtcgttcgt acggcccatg gcggcgatga
     4441 agtccggcgc aggcggcggc agcggcgccg tggcgatgcc gtcgacgata tggtcgagcg
     4501 gaaagtgcag ctcgtgcgcc ttatgcaacg cgacctcgag gcagcgcgcc gcgatctgca
     4561 ccgtgcccgc caggctcgag gtcggcgcat agatgaaggt gaggcggttc ggcttgagct
     4621 tgcaggcctt ggccaccatt tcgaccagcg ccggaggcgg cgggcggtcg gcttcgagca
     4681 cgagcacggc gcttgcggcc gtgtcgcgat agccgagctc gtcgaacagc ttctcagacg
     4741 aaccgatggc gcgcgccggc ccggacccga gcacgctgta tttcttgccc ttgacctcgg
     4801 ccgagaggct ccatccggca tattgcgagc cgaggcaggc aagcaccggc tgcgacgtat
     4861 gcaccgtcac catgaacggc caggtgctgt caggctctgt cgtcgcgagc gacacactgc
     4921 cgaggccgcc gaggcagatc tcgccgatcc gccgcccggc ctccagaccg cccacggcgt
     4981 tgacgccgca atcgatccgg cattcgccga gcgtcccctc cgtcacatcg acgcgaagcg
     5041 cgtcggcatc agcgatgagc gccttgacga gaggctccgc ctcgacgttg acgctcggcc
     5101 aggctcttgc tgcgcttcgt gaagcggctc gtctctcgct gtcgttcacc cgcgtcactc
     5161 ctgctaatac cctgaaccaa tttcagaatc cggcgccttc gcgcctcgac cagccgtttg
     5221 gctcctgccg ccgtgccggc gcgggccaac acagtgcata tcggacggtt cttgtcgatc
     5281 cattccgacg gctttgggcg atcggcgacc caggcgggcc acgtggtgac cgcgggcgcg
     5341 gcgctgccgc gcgtggcata gacgatcgcc gacgccatcg cgtcctgaaa cttggagacg
     5401 cgtgccggca acttgccttg acctaccgcc tcaagatgaa ggcgaaggag cggtctcgcg
     5461 ccgcgatcga agatgtcgag cgtcgcaccg ggacgcgggt tgatctcaag gaggagggga
     5521 tcttcgtcgc gcacgacgaa atcggcggaa gccatcccct tgatcgcgta ggtgcgggca
     5581 accttgacga cggccgccgt catctgccgc gccaccggag cggagatcgc taccggaccc
     5641 acagcgccgc catagcgcca gggacttgcg ggccggggcg cggtccattg ctcgctgaaa
     5701 ccgagaacgc gggcgccgct cccatttgca atgaagagcg cggacacggc acgccccgcg
     5761 acgcgttcct gaaagtaggt attggcggcg gccttcgcgc gccggcccgg aacgatgtga
     5821 cttccgccgg cgccgccgcg cttcttggcg agccaggcgc cgcgtgcccg aggctcgctt
     5881 gacgtcggcg gatgggcgat gccgaggcgg tcgagtgccg gaaaaaactg ctcgggcgat
     5941 ttgaggcgcg ccaccgtcgc ggcgtcggtc ccgagcagag gccagcgctt ggcgatcagc
     6001 ctcagcacct ccggccgatc ctcgaacccg gaaccgtaga cgagcccgag aacgggagag
     6061 ggggcaagcg cggcgagcgt ctggagcgca ggatagagcg tctgccagcg gaacccgaag
     6121 gcgatgctgc cgggcagttt gcggcaagca tgagccgcct cttgcgtgtc ggcgtcggca
     6181 aagaaatcgg cgaccagcgg cgtaaatccg gcgcgacgcg ccgctcccgc gagcgcccgt
     6241 cccgagatgc tcgcgatcag cagcgagcct ccctcacgcg gcgagtttct gggcaagagc
     6301 gtaagcgtcc cggaagtcga gatagagcgg tttctccgat tcgatcatct gcttgaagag
     6361 gccggcttcg gtccggtatt tgatgttgcc gaccgcgagt gcgccgatgc cgaccgcctt
     6421 cgttcctttg atcggcttgg cgtcgtcgtt cactcccacg ccctcgacgc ctgccggcgg
     6481 caccgcattc acgtcggccg ccacgagaag gttcttcgcc tccttcagct ggtcgaccgt
     6541 gagcagcctg cggccggcgg gtcctgcgga aaaaatgatg tcggcatcgc gcgcaagcgc
     6601 cgctttctgc tcctcggtac tgccgtcggc aaagctgaca tcgacaccga aacgcgctcg
     6661 cgcttcgtcg gccaaccgac gcacgcgctc aggcccgtca tatccgacca ggatcgacga
     6721 cgcgccgtcc atggcggcga tcacggctga agcgaaggcg acaacgccgg ttccgccgaa
     6781 caccacgacg cgcttgccct tcagtccggc cgagaacttc tctttcagcg cttccttggc
     6841 gcaagcgacc atggccgcgg cggtcgtgaa cgatcccgcg ggatcggcaa acacggaaat
     6901 ctcgaagggc ggcaccatag ccttcttggc gcggtccatc atgtcgagcg cttccatggc
     6961 gcgcttgccg ccgatgaaca cgccggtgcg cttcaccccc tcggggccgc gcgagaagat
     7021 cgcatcctgc acgagccccg ccacgtcctc gacggtgacg ttggtatagg gaatggcggc
     7081 atcgaaaccg gcgtcgagcg ccatgttcac gtcgaacggg ctcatatgct tgagcggcgt
     7141 gatcatgtgc agaatgcggg ggttccccat cgtcgtttcg tctccctttc aagccaagcc
     7201 cgctggcttg gggctcaagt cgcgggcttc ggaggcaccg ctttagcaca atcggcggcg
     7261 gggccaagct cctacgatgg cgggagaccg aaatgggcgg tgcccaaccc ggcggtcagt
     7321 tgacatcttc ggagatctcg atccgcgcac cttcgttgcg gccttgtgcc agcgcgaagc
     7381 tcagtccggg atgcttgcaa cgggcccgca atcggtcaag caaagcctcg ccctccgcct
     7441 gtgccgctgc aaaggcgaag ccggtcggac cccacgaact ctgtccaagc ccgacgatcc
     7501 cttggtcgcg aagccaagca agcacctcgg caacgcgcgg gctcgcatag gccccccctt
     7561 gcgagggcgc gaaataggca cccatgcgcg cttgcagatc gccgacatgg cggcagaagg
     7621 ccgcgaaatc gcccgccgcg agcgcaggca gcgcgccttg cgtgatcgct tgcgaaagcg
     7681 cttcgatctc gcgcggggga aaatcgggca gcgtctggaa cgccgcgatc tcgttcgcgc
     7741 ccacgagccc tttcgccgcc tcatcgaaga tcaggagcac gcgccacgaa gacgggaagg
     7801 gaacgcggct caaaagcttt ggtaaattcc cgtcgagcgg cccaccgtcg agcacggccc
     7861 cgccttgcgc gaaggtggcg atgccgatgc ccgaacgggc gccgcgcccg agccgcgccg
     7921 cgacctcttg cggttcgagc cgaagccctt cgagggcgcc gaaggcggag ccgacggcaa
     7981 gggcgagctg ggtgccggaa ccgaggcccg catgcggcgg gatcgcttcc tcgatccgaa
     8041 gggcgtaggt ctgttcgatc ccgcagctcg ccgcgatctc gttgagatac cgcaaggcgc
     8101 gctcccgttc cggcccgctc gtcgcagact tctccgctcg ccttaggacc agccgggtcc
     8161 gcggctgatc gagcgagagc ccgaacgagc cgaacgggcg ggcgctgcgt ccacttggat
     8221 cgagaaagcc gaaatgaagc cgcgcggtcg cggtgacaat cgcctcgcgc gggcgcgctg
     8281 gcgccgggct tgcatgtctc ggctcacggg gaaaggcctc cattgatcgg cccatatcct
     8341 ctcgctatgc ttcggctgaa agggaaggcg agtactcttt caaaagagaa ggacgatggc
     8401 aacggccgag cggacctata gcaatgccga ggtcgaggag cggctcaaga aggagctgcc
     8461 ccattggtac ctcgaggacg gctggatcag gcgccgctat cgcaccaata gctggaaggg
     8521 cacgctgatg gtgatcaacg ccgtgggtca cctgtccgag gccgcctggc atcatccgga
     8581 catcaccgcc tcctatgcct gggtcgaggt gaggctcatg aaccacgccg ccaagggcat
     8641 caccgacaag gacttcgagc tcgccaagag gatcgaggac ctcgtccagt ggcagcccgg
     8701 caaggagggc ggcgcgttgg aagggacccc ggccgaggac atgcgcttcg cctacatcaa
     8761 atacgacaag cccaaagcct gaccgtttag gccctgaaca tcgcgcctct gttgaatggg
     8821 acccccaagt cccgggccca cggccgcttg cgccttggaa ctcacctgtt taatggtgcc
     8881 cggctgaatt cccgcaagag ggggcggcct tgccatgacg gaattgaaga tcaagggcgt
     8941 caccatcgtc gagacgttcg ccgaggcctt tcccatggtg ggcacgcgga tcgtcatcac
     9001 ggcgccctcc ctcgaatggg ctctgaccgc cgcgcgcacc atgaccggct ttgccacctc
     9061 ggtgatcgca tgcggggctg aagccggcat cgagcggacg ctctcgcccg acgagacgcc
     9121 ggacggccgt cccggcgtcg ccgtcctgct cttcactatg gacaccgaga agctccagcg
     9181 ccagctgcgc aaccgtgtcg gccagtgcgt gctgacgagc ccgggctcgg cctgcttcgc
     9241 cggactcgat ggcgagaaga agctgaagct cggggcgtcg cttcgcttct tcggcgatgg
     9301 ttggcagatg tcgaagctga tgggagggcg gcggttctgg cgcatcccgg tcatggacgg
     9361 cgaattcctg tgcgaggcga cgaccggcct caccaagaag gcggtgggcg gcggcaatct
     9421 cctcgtgctc ggcaagtcgc atggggcggt gctcgccgcg tgcgagcgcg cggtcaaggc
     9481 catcgccgaa atcgagggcg cgatcacgcc gttcccggga gggatcgtgc gctctggctc
     9541 gaaggtcggc tccaaataca agggccagat cgcctcgacc aacgatgcct attgcccgac
     9601 gctcaaagcc gccgtcgata cggcccttcc gctcgaggtc gaagcggtgt tggagatcgt
     9661 catcgacggg ctgaccaaag actccgtcgc aaaggcgatg cgtgccggct gccatgccat
     9721 cgccgaaggc ggcgccgaca cgggcgtcac ccacatcacc gccggcaact acggcggcaa
     9781 tctcggcccg catcatttcc atctcaagga gatcgtcgcg tgagcgcgct gatcttcgag
     9841 ctgagaagcg cgcccgatga gaggctcgat ctcgcgccgc tcacaccgga acggctcgac
     9901 ggcctcaagt cgaaggagat cgaggcgctc gcgatcggga ccacgcgcgt gccgttgacc
     9961 gtcggcgacg ccttcaagct caagggcaac gacccggccg agctcagatt cgtcggcacg
    10021 gacatgcgct gcgacaagat cggcgccggc atgaccaagg gcgcgattat cgtcgatggc
    10081 gatgccgggg cctatctcgg cgccggcatg aagaagggga cgatcgaagt caacggcaac
    10141 gccggcgttt tggccggcgc tagcatggcg ggcggcacca tcgccatctc tggcgatgcc
    10201 ggcgagcgcg gcggcggcat caatgtcggc gagaccctcg gcatgagggg aggggtgctc
    10261 accatcggcg gcaaagcggg atcgttgctc ggcgaacgca tgcggcgggg actggtggtc
    10321 gtggggggcg acgcggggga ctatgccggc gggcgcatgg tcgccggaac cattctcttc
    10381 aagcggagcg tcggcgccaa tgccggtttt ggtctccgcc gcggcagcct gatctttgtc
    10441 gaggagccga aagacgtgtt gccgaccttc ggcgattgcg gcgtgctcga attcgactat
    10501 ctcaggcttc tggagaactg gcttcgcgat aacggcaaac cggtcaacct tggctcgcgc
    10561 gcccgcaggc tcatgggcga catgtccgtg ctcggcaagg gcgagatgct gatccttgcg
    10621 tgagcctgcc gcgaacccat attggctatg ctcccaagcc atgcggatct tgcgtgatcg
    10681 gcttgcacaa tgactggttc cccttacgtc cttcttgacg acagcctgac gccgggcggc
    10741 cgcagcctgc tctacaccgg cgcggagcac gtcgttgcgg cctacacccc tgccgaggtc
    10801 gaacaggcgc tggagcaggt cgaggcgggg cttgcgcgcg gcctccatgc cgcaggcttc
    10861 ttcgcctacg agcttggcta ttgtctggag ccgaagctca ggcacctcat tcccgacggc
    10921 cgccgcgtac cgttgttctg gctcggactc ttccgcgcac ctcttccgct cgacgatccc
    10981 ggcacccggg cctggctcgg gacccacgcc gcggccggac aggccacgat ctc
//
LOCUS       DASUQP010000170         6275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1993211, whole genome shotgun sequence.
ACCESSION   DASUQP010000170 DASUQP010000000
VERSION     DASUQP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993211"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFY74_07465"
     CDS             <1..345
                     /locus_tag="VFY74_07465"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5931571.1"
                     /translation="DIVKRAVPGAEELISYQIPAYRHGGGYFLYISGWKAHYSLYPAT
                     EALVAALKDDIAPHLANKNTIRFKLSDPVPGRLIERIAKLRAKEVAEKAKAKASKIKA
                     SKIKIAKSKAKR"
     gene            complement(381..746)
                     /locus_tag="VFY74_07470"
     CDS             complement(381..746)
                     /locus_tag="VFY74_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931572.1"
                     /translation="MAVLDESENRPSTGTVFTPRQVRVLKIAVIVMGLLLVGGFAFVL
                     AAIVYQASRGGQDGGVSAEMPGHVEAELAIAKDASVTALSLDGDRLALHLNSAAGPEI
                     AVVDLKTGTIVARIRLKPE"
     gene            761..1774
                     /locus_tag="VFY74_07475"
     CDS             761..1774
                     /locus_tag="VFY74_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5931573.1"
                     /translation="MPQSPKSPALKRLQAEAEASHEGLRLDRFLSERLPQLSRTRVQS
                     LIKEGRVSLGGATIIDVKYRVKPGERFRLDLPPAAAPEPRGESIPLDVIFEDDALIVI
                     DKPAGLVVHPGAGHPAGTLVNALIGHCGASLSGIGGVARPGIIHRLDKDTSGLMVVAK
                     TDETHRALAEQFADHGKSGVLERGYVALVWGVPARPYGTIDAPIGRHPTSRTKMAVLR
                     SKGRAAATHWRVIATFGRGKEKIASLLECTLETGRTHQVRVHLAHIGHPLIGDPLYGP
                     GFKSKLRKLPEPVRGKLEALDRQALHAAKLAFAHPLTGTLLKFNSPLPRDLSEIVEAF
                     KEV"
     gene            1888..2784
                     /gene="rpoH"
                     /locus_tag="VFY74_07480"
     CDS             1888..2784
                     /gene="rpoH"
                     /locus_tag="VFY74_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX5931574.1"
                     /translation="MAARSLPSVGAQGGLTRYLEEIRQFPMLEPQQEYMLAKSWREHE
                     DRDAAHQLVTSHLRLVARIAMGYRGYGLPIGEVISEGNVGLMQAVKRFDPDKGFRLAT
                     YAMWWIRAAIQEYILRSWSLVKMGTTAAQKKLFFNLRKIKGQLKALDEGDLRPDQVKR
                     IANQLGVTEEDVVSMNRRLAGDSSLNAPVRNDAESGEWQDWLVDDTMDQETALAESEE
                     RDRRRGMLNDALKGLNARERRVFEARRLAEEPLTLEELSAEFGVSRERIRQIEVRAFE
                     KVQKAVQKAATAASQREPAPAQ"
     gene            2826..3644
                     /locus_tag="VFY74_07485"
     CDS             2826..3644
                     /locus_tag="VFY74_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5931575.1"
                     /translation="MTAMKRLAVNAIEIAYLDEGNGPAIVLGHCSSASHKEWLPLIET
                     LKSDWRVLAPDFIGYGQSEPWPATEPFSIDADIEVLRAVAKKAKGPLHLVGHSYGAAL
                     ALEAARTLGSRVRSLTLVEPVSFHLLRLEGRPEWAEVEGLGRAVLGAVAKGEDREAAK
                     AFMTYWLGRTRWWLSPDRFKSAIAATIPKVALEFSIAIDAKTTLQDYAGITAPTLLIA
                     GSKTRAPTRAVVDLLAATLPNARLAILPGAGHMSPFTHPAELNRLIIDYLCSER"
     gene            complement(3616..5463)
                     /locus_tag="VFY74_07490"
     CDS             complement(3616..5463)
                     /locus_tag="VFY74_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX5931576.1"
                     /translation="METAQLIQVAVFLAAAAIAAPLGRFLRMGAVLGYLAGGVIIGPY
                     VLGPLYALDDVGELLHFGEFGVVMLLFVIGLELRPVRLWSMRSAIFGLGTAQLVATAA
                     MLAAIGIGLGLPAAQALFIGLALSLSSTAFALQVLEEKGELTTRHGRLAFSVLLFQDL
                     AAIPLIALVPLFAFGADEPSMDISKALIAIGTILGVIVAGRFLLNRLYRIVAATGVRE
                     AMTASVLLTVVGVALVMEAAGLSAALGAFIAGALLADSEYRHQIEADIAPFEGLLLAV
                     FFIAVGMSIDLGVLVGEPVKLFALVLGLVALKAAILYLLGRWWGLDNAAARRLGLVIS
                     QGGEFAFVLFGAGALEGVIDRQTANFLTLAVTLSMAATPLLLLIDDAINRALKPAPPE
                     YEMPPDSDQHVIVAGFGRFGQIVARVLRARRIPFTALDSNIEQVDFVKRFGAQIYYGD
                     AGRLDILRAAGVDKARAFVLAIDNVESSLRVAEIVRSNFPDLTIYARARDRTHVHKLM
                     DLGVTIIERETFLSALALTKTLLRGLGLREPEVKRLTETFKQQDERRLYQDYQYYTDL
                     EKVRANAQSQAKELEELFARDIEELPAEERSPQPERAAERVSAQNRDSR"
     gene            6068..>6275
                     /locus_tag="VFY74_07495"
     CDS             6068..>6275
                     /locus_tag="VFY74_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5931577.1"
                     /translation="MLGRAAMGPHETSPKPSFTEALALWGRIGILSFGGPAGQIALMH
                     RLLVDERRWITEERFLHALNFCMLL"
BASE COUNT         1180 a         2059 c         2021 g         1015 t
ORIGIN      
        1 gatattgtga agcgagccgt gcccggcgcc gaggagctga tctcatacca aatccccgcc
       61 taccggcacg ggggcggcta ttttctctat atctcgggat ggaaggcgca ttattcgctt
      121 tatccggcga ccgaagccct cgtcgccgct ctcaaggacg acatcgcgcc ccatcttgcc
      181 aacaagaaca ccattcgctt caagctctct gatcccgtcc ccggtcgact gattgaacgg
      241 atcgcgaagc tccgtgccaa ggaagttgcg gagaaagcca aggccaaggc ctccaagatc
      301 aaggcctcca agatcaagat cgccaagtcg aaagcgaagc gatgacctga gccgcgcgcc
      361 gccgatgcgg gtccgatggc ctattcgggc ttcagcctga tgcgggcgac gatcgttccg
      421 gtcttgagat cgaccacggc gatctcgggg cccgccgccg aattgagatg gagtgcgagc
      481 cggtcgccat caagagagag cgccgtgacc gacgcatcct tggcgattgc gagctccgcc
      541 tccacgtgac ccggcatttc ggctgaaaca cccccgtctt gccctccgcg cgaggcctga
      601 tagacaatgg ccgcgagcac gaaggcgaag ccgccgacca gcaacaaccc catcacgatc
      661 acggcgattt tcagcacccg cacctgccgc ggggtgaaca ccgtgcccgt tgacggccta
      721 ttctcggact cgtccaggac cgccattcct cgaagacctc atgcctcagt caccaaaatc
      781 acccgccctc aagcggcttc aggccgaagc ggaggcgagc catgaaggct tacggctcga
      841 ccggttcttg agcgagcgcc tgccccagct gagccgcacc cgcgtgcaga gcctgatcaa
      901 agagggtcgc gtctcgttgg gcggggcgac cataattgac gtgaaatacc gggtcaaacc
      961 gggcgagcgc ttccggctcg atctgccgcc ggcggcggcg ccggagccgc gcggcgagtc
     1021 gatccctctg gacgtgattt tcgaggatga cgcgctgatc gtcatcgaca agccggcggg
     1081 actcgtcgtc catccgggcg ccggccaccc cgccggcacg ctcgtcaacg cgctgatcgg
     1141 ccattgcggc gcgagcctct caggcatcgg cggtgtcgcg cgccccggca tcatccatcg
     1201 tctcgacaag gacacgagcg ggctgatggt cgtggccaag accgatgaga cgcatcgtgc
     1261 gctcgccgaa caattcgccg accatggcaa atctggcgtg cttgaacgcg gctatgtcgc
     1321 tctcgtttgg ggcgtccccg cccgcccgta tggcacgatc gatgccccca tcggccgcca
     1381 cccgacgagc cggaccaaga tggcagtgct gcggtcgaag gggcgcgctg ccgcgaccca
     1441 ttggcgtgtg atcgcgacgt tcggccgcgg caaagaaaag atcgcgtctc tgctcgagtg
     1501 cacgctcgag accgggcgca cccaccaggt gcgcgtgcat ctcgctcata tcggtcatcc
     1561 cttgatcggc gatccgctct acggcccggg cttcaagtcg aagctccgca agcttcccga
     1621 accggtgcgc ggcaagctcg aggcgctcga tcgccaggcg ctccacgccg caaagcttgc
     1681 cttcgctcac ccgctcaccg gcacattact caaatttaac agtccacttc cccgcgatct
     1741 ctctgagatc gttgaggctt tcaaggaagt gtgaagacat cgcggtcttt tttcgcaagc
     1801 gcaaaggctc tatagagagg aacgccgaag ggcggagtga gtttggcacg gttgtggccc
     1861 cgtaagggcc gctggagacg ggttttcatg gctgcgagga gtttgccgag tgttggcgcc
     1921 cagggcggac tgacgcgcta cctcgaagaa atcagacagt ttccgatgct cgagccgcag
     1981 caggagtata tgctcgccaa gagctggcgc gagcatgagg accgcgacgc ggcgcatcag
     2041 ctcgtcacct cgcacctcag gctcgtggcg cgcatcgcca tgggctatcg cggctacggc
     2101 cttcccatcg gcgaggtgat ctcggaggga aatgtcgggc tgatgcaggc ggtgaagcgc
     2161 tttgatcccg acaagggctt tcgccttgcc acttacgcca tgtggtggat cagggccgcg
     2221 atccaggaat acatcctgcg ctcctggagc ctcgtgaaga tgggcaccac ggcggcgcag
     2281 aagaagctgt tcttcaacct gcgcaagatc aagggccagc tcaaggcgct ggacgaaggc
     2341 gacctcaggc cggaccaggt gaagcgcatc gcaaatcagc tcggcgtgac cgaagaggac
     2401 gtcgtgtcca tgaaccgccg gctcgcaggc gacagctcgc tgaacgctcc ggtccggaac
     2461 gacgccgaaa gcggcgagtg gcaggactgg ctggttgacg acacgatgga tcaggagacc
     2521 gcgctcgcag agtctgagga gcgcgaccga cggcgcggga tgttgaacga cgcgctcaag
     2581 ggcctgaacg cgcgcgagcg ccgtgtcttc gaagcgcgca ggctcgccga ggagccgctc
     2641 acgctcgaag agctgtccgc agagttcggc gtcagccgcg agcgcatccg gcaaatcgag
     2701 gtgcgcgcct tcgagaaggt gcagaaagcg gtacagaaag cagccacggc ggcgtcccaa
     2761 cgcgagcccg ccccggccca gtgacctctc cctcccccgc ggtggagaaa gcgccggctc
     2821 agggcatgac cgccatgaag cggctcgccg tcaacgccat cgaaatcgcc tatcttgacg
     2881 agggcaatgg tcccgcaatc gtcctcggtc actgctcgag cgcatcgcac aaggaatggc
     2941 tgccgctgat cgagacgctg aaatccgatt ggcgcgtgct cgcccccgat ttcatcggct
     3001 acgggcagtc ggagccttgg ccggccaccg agcccttttc cattgacgcc gacatcgagg
     3061 tgctgcgtgc cgtcgccaag aaggcgaagg gcccgctcca tctcgtcggt cattcttacg
     3121 gcgcggcctt ggcgctggag gcggcgcgga ccctggggtc acgcgttagg agcctcacgc
     3181 tcgtcgagcc ggtctcgttt catctgttgc ggctggaggg aagacccgaa tgggccgagg
     3241 tcgaagggct cggccgtgcc gtgctcggcg ccgtggcaaa gggcgaggac cgcgaagcgg
     3301 ccaaggcctt catgacctat tggctcgggc gcacgcgctg gtggctttcg cccgaccgct
     3361 tcaagtcggc aatcgccgcg acgatcccca aggtcgcgct cgaattctcg atcgccatcg
     3421 acgccaaaac gactttgcag gattatgccg ggatcaccgc gccgacactt ctgatcgcag
     3481 gcagcaagac gcgggcgccg acccgcgccg tcgtcgatct cttggccgca acgttgccca
     3541 acgcgaggct cgccatcctc ccgggcgcgg ggcatatgag ccccttcacc catccggccg
     3601 agctcaaccg cctcatcatc gactatctct gttctgagcg ctgacccgct cggcggcgcg
     3661 ctcgggttgc ggactccgct cctcggccgg caactcctcg atatcgcggg cgaacaactc
     3721 ttcgagttcc ttcgcctgcg actgtgcgtt ggcgcgaacc ttctccaggt ccgtgtagta
     3781 ttgatagtcc tgatagagcc gccgctcgtc ctgctgcttg aaggtttcgg tcaggcgctt
     3841 gacctcgggc tcgcgcagac ccaggccgcg cagcaatgtc ttggtcagcg ccagcgccga
     3901 caagaaggtc tcgcgctcga tgatggtcac gccgagatcc atgagcttat gcacgtgggt
     3961 ccggtcccgc gcccgcgcgt agatggtaag atcgggaaag ttcgaccgca cgatctccgc
     4021 gaccctgagc gaactctcga cattgtcgat cgccagcacg aaggcgcgcg ccttgtcgac
     4081 gcctgcggcg cgaaggatgt cgaggcgccc agcgtctcca tagtagattt gcgcgccgaa
     4141 gcgcttcacg aaatcgacct gctcgatatt ggaatcgagt gcggtgaatg ggatgcggcg
     4201 ggcgcgcagg acgcgggcca cgatctgccc gaagcggccg aagcctgcga cgatgacgtg
     4261 ctggtcgctg tccggcggca tttcgtattc gggcggcgca ggcttgagcg cccggttgat
     4321 ggcgtcatcg atgaggagga ggagcggcgt cgcggccatg gacagggtta cggcaagcgt
     4381 caaaaaattg gccgtctgcc ggtcgatgac gccttcgagc gctcccgccc cgaacagaac
     4441 gaaggcgaac tcgccgccct ggctgatcac gaggccaagc cgccgcgcgg ccgcattatc
     4501 caatccccac catcggccga gcagataaag gatcgcggcc ttgagggcga cgagcccgag
     4561 gaccaacgcg aacagcttga caggctcgcc cacgagcacg ccgagatcga tcgacatgcc
     4621 gacggcgatg aagaacacgg cgagcaacag cccttcgaaa ggcgcgatgt cggcctcgat
     4681 ctgatggcgg tattccgagt cggcgagcag cgcgccggcg atgaaggcgc cgagcgcagc
     4741 cgagagcccg gccgcctcca tcaccagagc cacgcccacg accgtcagaa gcacgcttgc
     4801 cgtcatcgcc tcgcgcacgc cggtggccgc gacgatgcgg tagagccggt tcaacagaaa
     4861 gcgcccggcg acgatcaccc cgaggatggt gccgatggca atcagagctt tgctgatgtc
     4921 catgctcggc tcatccgcgc cgaaagcgaa cagcggcacg agcgcgatca gcgggatcgc
     4981 ggcaaggtcc tggaacagca gcacggagaa ggcgagccgg ccgtggcgtg tcgtgagctc
     5041 gcccttctcc tcgagcactt ggagtgcgaa cgccgtcgag gagagcgaga gcgcgagccc
     5101 gatgaacagc gcctgcgccg cgggcagccc aagcccgatg ccgatcgcgg caagcatcgc
     5161 ggcggtcgcg acgagctggg ccgtgccgag cccgaagatc gccgagcgca tagaccatag
     5221 cctgacgggg cgaagctcaa gccctatcac gaacaagagc atcacgacgc cgaactcgcc
     5281 gaaatggagc aattccccca cgtcgtccag cgcatagagg ggcccgagca cgtaaggtcc
     5341 gataatgacg ccgccggcga gatagccaag cacggcgccc atgcgaagga atcggccgag
     5401 aggggcggcg atggcggctg cggcaaggaa caccgcgacc tgaatcaact gtgccgtttc
     5461 catgacgaat ccttcgcgaa tgcgtccggt tggggtagca cgcggatgga aaactcacta
     5521 gccgggacca cgcacccgct gctgatccgt ttctcgcgct ctcccccctg ccttcgccga
     5581 agcgaagccg cggcggcttc gcgcaggcag gttgagggag agcacgcgcg ccgtttggcg
     5641 cgcggtgagg ggtgagcggg cgtaagcccg cgtcaaaaga gattcacccc cctcaccctt
     5701 cccgccattc gtcgcgttgc gcgcgccggg cgggaagccc tctcccacga gggagaggga
     5761 aaagtcgtgc tttcgagcct gcacgaccgc gactagcggc aaccctctac gctcctcgtc
     5821 attgccgggc ttgacccgcc tacgcgggcc gaagcctcgt gaccactccc gccagccatc
     5881 gcttcggcgg gcgaaggccc ggcaatccat tccattggcg cttcatctct tggagacgaa
     5941 acggcttgga tgcccggaca agcttgcgcc tgggccggcg aagccggacc cgggtgtctg
     6001 gcatgacgaa ggttgcgggc ctcgggggag gaagggccac ggttaggact gcgcgcggcc
     6061 ccggtccatg ctagggaggg cggcgatggg gccgcatgaa acttcaccca agccaagctt
     6121 tacagaagcg ctcgcgctgt ggggcaggat cggcatcctg tccttcggcg gccccgcggg
     6181 tcagatcgcg ctgatgcatc gactgcttgt cgatgagcgg cgatggatca ccgaagagcg
     6241 ctttctgcat gcgctgaact tttgcatgct tcttc
//
LOCUS       DASUQP010000171        16743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1856147, whole genome shotgun sequence.
ACCESSION   DASUQP010000171 DASUQP010000000
VERSION     DASUQP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 16743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16743
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856147"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFY74_07500"
     CDS             <1..1333
                     /locus_tag="VFY74_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5931578.1"
                     /translation="IRDSGTKVMVTLDLALLFEKVEAMLARGILEKAVVARFPSLLPP
                     LKSAGFKLLQRTKLANPAASSARDRIVPERTVLANDGRYQRPPITPDAIAVLQYTGGT
                     TGTPKGAMLSHANLTVNVGQVIAWRNRQATEADRILGILPLFHVFAMTTVMNYGIAGG
                     MELILLPKFELVPTLKLIGKLRPTMMPGVPTLYNAMLRHPKIAKFDLSSLEFCISGGA
                     ALPVEVKREFEAMARCGLVEGYGLSETAPVATCNPVRNGKEGSIGLPLPATEISIRSL
                     EDPTQEMPRGEPGEICIAGPQVMSGYWNKPQETEAAFVGRYFRSGDVGYMDEDGYIFI
                     VDRIKDMINCSGFKVYPRRVEDALYAHRAVAEVTVVGIPDHYRGEAPKAYVKLKEGMA
                     ATAAELHEFLREKLSKFELPAEIEFRDELPKTMVGKLSKKELRAEGQRQSR"
     gene            complement(1374..2678)
                     /locus_tag="VFY74_07505"
     CDS             complement(1374..2678)
                     /locus_tag="VFY74_07505"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX5931579.1"
                     /translation="MKHHACAAVIGLLTAALFSPVSQSHAASADPTGYWMKPDAERES
                     KIHVFKCGTKRTNLCAKIAWLKDPNDRKGQPLHDVRNQNPSQRGRQIMGLPIFSQLVP
                     SAPSTWTGKIYNPEDGQTYSATLTVVSRKQLLLKGCKAWLLCGERTWYRTSPPPAEVT
                     PEAPADGSQQIEASVTPQLPEAAPPSAAAAVPAEPASVPSADATVEKASIGAAEAQQE
                     MQAMTTPQMTTPQLPAEPSLTEAPAVAPEPVTPVTQEIASPTESPTEIDAGRGYGFLA
                     VSPDADTTARLSGENVPSMMLMAKPIATEAAAPSTAQPAAKSAAVPAKPKAQPKPVAT
                     AAAKPVATPKPVATAAKPVATAAKPSPKAEPAAKSTEQATPAPDDRDAAEAEAASDDT
                     APAETADATLAQPVPLTRRERRQLRRQQREMGIQEPLLPWWR"
     gene            complement(2829..4139)
                     /locus_tag="VFY74_07510"
     CDS             complement(2829..4139)
                     /locus_tag="VFY74_07510"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5931580.1"
                     /translation="MAEASMRRVAVLGGSRTPFCRSHTAYVELSNLDMIVAALGGLID
                     RFALHGTHIDEVVGGAVVTHAKDWNLAREAVIGSALAPTTPGITMMQACGTSLQGALG
                     LAAKIATGQIESGIAFGSDTTSDAPIVFQRKFAQRLVSMSRARSLGQKAASFKGFTPA
                     ELAPQPPSTSEPRTGLSMGEHCELMAKEWGISREAQDRLAYESHKKAAAAYDEGFMDD
                     LVIPFAGVFRDNNIREDISLEKMAELKPVFDRSGAGTITAANSTPLTDGAAAVLLASE
                     EWAEKRGIPVQAYLGLGRTSAVDFVQGEGLLMAPTVAVSDLLQQTGLALQDFDYYEIH
                     EAFAAQVLATLRAWESEDYCRRRLGRSEALGSIDLAKLNVKGSSIAFGHPFAATGARI
                     VAVLAKLLHSEGGRRGLISVCTAGGMGVAAIVEGVETRTGAGAA"
     gene            complement(4169..6154)
                     /locus_tag="VFY74_07515"
     CDS             complement(4169..6154)
                     /locus_tag="VFY74_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5931581.1"
                     /translation="MPTTLPDLQDWRFTVDFEGIAWAVMDRQGESMNSLSRRATEELG
                     EIVMAVEQAAASGEVKGLVLMSAKERSFIAGADIREFENFDTEQKIEDVVKQTLELLN
                     RIDRLPVTVVAAIHGYCLGGGLELALACDWRIADREEATRLGFPEVKLGIFPGLNGTV
                     RAIEAAGPLDAMTAMLTGRMLRPTAARAMGLVDQLVATRHNLRWAARKAVLQKRKSRG
                     APWWKRLMLKQPARGLLARQMRAKTAAKVREEHYPAPFRLIDLFERYGDDPARMRIAE
                     TEMFTPLMASEASRNLRRVFKLSEMLKDEAPKNGFKPRRVHVIGAGTMGGDIAAWCVV
                     QGMQASLQDLDEAQIAKALARAKSLFKRQLRTPLAIDTAAARLIADPSGRHIKQADVV
                     IEAIVEKLDVKQSLFRALEREVKPRAVLATNTSSLRLEDIARPLADPGRLVGLHFFNP
                     VAQLPLVEVVRGERTREEEVRKACAFVNAINKLPLIVKSCPGFLVNRVLAPYMMEAVR
                     RYQLGEPREKIDQAALKFGMPMGPLELMDMVGLDIANHVGEELDLAPEGDNVLAALVK
                     QGKLGKKTGEGFYLWQGGKPKREPVAYDQAELERLGRELVKPMLDEAERALSDRVVAS
                     ADHVDAGVVFGTGFAPFRGGPLHYRRKEARAATAAAA"
     gene            complement(6164..8677)
                     /locus_tag="VFY74_07520"
     CDS             complement(6164..8677)
                     /locus_tag="VFY74_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5931582.1"
                     /translation="MAEVIFVLVFILAAFALAMNRASLRVWALAVALFTLGAQMGLAG
                     GHFHWPVFTWWSLFGWLAAGLLFLASLPDIKRNYIVMPAYRALKGVMPAISETEREAL
                     EAGTVGWDAELFSGTPDWEKLRRVAPVTLTAEERAFLDGPTAELCRKLDDWRIRHELH
                     DIPADIWRFVCERGFLGMLISKEHGGLGFSAQAQSLILGQISSRSPGGVTIVMVPNSL
                     GPGELIEKYGTDAQKEHFLPRLARGEEIPCFALTGPFSGSDAAAMQDIGVVTRGEHEG
                     RDTLGIRLTWDKRYITLAPNATLLGLAFRLFDPEKHLGRGEDVGITLALIPTNHPGVE
                     IGRRHLPGGTGFPNGPTRGRDVFVPIDWIIGGEERAGQGWRMLMSCLAAGRSISLPAT
                     SAAAAKALLRASTAYARIRKQFNLPIGYMEGVEEPLARMIETAYALEAARALTASMVS
                     AGEKPAVLSALLKYVSTERMRQSVNDALDIHGGKGICDGPSNYLQGAYQMTPVTITVE
                     GANILTRTLITFAQGALRSHPYLFTEIEALQDPDRARGTERFARAFEDHVAFTVSNAA
                     GALFHNLTFGLFASAPPNAGDTRALWRELARASRSFALVADATVALLGGGLKVKQKIT
                     GRMADALAELYLLSSVLKRYDDDGRPKEDLKVVEYCARNCLYRFDQALVGTLRNFPVR
                     WAAWAMAPLVFPFGLRRRPASDEAGKQIVRAALEPGAFRDRLTRHIYATDDPKDRVGL
                     LDYTLAKAVACEEADRKLERAIRKGEVRRFHDHDWIAEAESKGVLSATEARALAELRD
                     LVARAIAVDDFAASELARGRSPAPPPQDSATRPENIAAE"
     gene            9021..9260
                     /locus_tag="VFY74_07525"
     CDS             9021..9260
                     /locus_tag="VFY74_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX5931583.1"
                     /translation="MAEPERPQLNPYQVELEAGRRYSWCRCGRSKRQPFCDGSHAGTG
                     IEPLTFTAERTELALLCGCKDTGDPPYCDGSHLLL"
     gene            9387..10301
                     /locus_tag="VFY74_07530"
     CDS             9387..10301
                     /locus_tag="VFY74_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715254.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEX5931584.1"
                     /translation="MAYFLQQLVNGIALGSIYGLIAIGYTMVYGIVGMINFAHGDIFM
                     VGAFIALILLLALLSVGVTMIPLALLIVLLLAMALTAVWGWTVERIAYRPLRESFRLA
                     PLITAIGISIILQNFIQVAQGARVKPLQPVVTGGHRVFEAGGFAVSISNIQILIVVTT
                     LVLMIVFWLIITRTALGRAQRACEQDRMMASLSGVNVDRTISLTFVLGAALAAVAGLL
                     YLLYYGVIDFYIGFVAGIKAFTAAVFGGIGSLPGAMLGGLIIGLIETFWSAYFTIEYK
                     DVAAFSMLAIVLIFFPTGLLGRPEVEKV"
     gene            10306..11673
                     /gene="livM"
                     /locus_tag="VFY74_07535"
     CDS             10306..11673
                     /gene="livM"
                     /locus_tag="VFY74_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEX5931585.1"
                     /translation="MVGPDKSFSHRLKSALLNATLSALVAFLLFCLILGVRTVDTVQG
                     LTLRPRLLLLAVAVGIVFVGRLALDLFWWNRNWNLPKPVGLKRLSDATAQYGGIALIV
                     TAVLLPIVALAIEKSLHLGLPLRYLFDLSIVVLTYLMLASGLNIVVGLAGLLDLGYVA
                     FYAVGAYSFALLATNFGWGFWMCLPFAGLFAALWGCTVGLPVLRLRGDYLAIVTLAFA
                     EIVRIVLLNWTELTNGPNGISGIPDPTLFGLAFTPRGDVTFSSFFGIRYEPLQGIVFL
                     YYVILALSLLTIFVTMRLRRLPVGRAWEAMREDEIACRSLGINTTVTKLSAFSTGAMF
                     GGFAGAFFATRQGFISPESFTFIESATILAIVVLGGLGSQLGVALASIALIGGFEALR
                     HTHGLKVIFGPDFDPALYRMLLFGLAMVIIMLWRPRGIVASRTPSILLEPQRGGVSLA
                     KERRA"
     gene            11670..12515
                     /locus_tag="VFY74_07540"
     CDS             11670..12515
                     /locus_tag="VFY74_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5931586.1"
                     /translation="MRGWQKDPILRVEHLTMRFGGLMAVDDVSFEAGRGDITALIGPN
                     GAGKTTVFNCITGFYKPTEGMITLTHPNGSSFYLERLDDHDINSRARVARTFQNIRLF
                     KGMTVLENLMVAQHQTLNRASGLTFGALLGVGGYRAAEKDAIAFARAWLSRVGLTSRA
                     DTPAGDLPYGDQRRLEIARAMCTRPLLLCLDEPAAGLNARESEGLNELLYSIRKQDGI
                     AILLIEHDMSVVMRISDHVVVLDYGKEIADGTAEEVRNDPRVIAAYLGVPEKELAKVE
                     REVGL"
     gene            12512..13258
                     /locus_tag="VFY74_07545"
     CDS             12512..13258
                     /locus_tag="VFY74_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5931587.1"
                     /translation="MSAPLLAIGNVTAGYGNIPVLHGVSLDISKGEIVTLIGANGAGK
                     TTLMMTIFGRPRAAQGRILFEGEDITHLPTHRIARLCIAQSPEGRRIFPRLTVLENLQ
                     VGALIANDPTAFENDLERVCALFPVLKQRKAQRGGTLSGGEQQMLAIARALMGRPKLL
                     LLDEPSLGLAPLIVKQIFETIGELNRQGLTVFLVEQNAYHALKLAHRGYVMVNGKITM
                     AGTGRELLEAEEVKAAYLEGGAHGAGQART"
     gene            13255..13599
                     /locus_tag="VFY74_07550"
     CDS             13255..13599
                     /locus_tag="VFY74_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931588.1"
                     /translation="MSWLSDDGLRVFLVLTVIIGGGAAFLGGRGLARSWKPFWRLFVY
                     MALLAAAIRFFHYALFDGALLSPYYYLVTYAVLLAAACLGFRTMRTTQMVTQYGWLYQ
                     RTSPLTWRDRRA"
     gene            13695..14822
                     /locus_tag="VFY74_07555"
     CDS             13695..14822
                     /locus_tag="VFY74_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5931589.1"
                     /translation="MKRMAGLIAALAFAVLAVGCGEDSNTTPIAMVGPVTGQYASFGA
                     QMQNGAELAVEDINAAGGVLGKKLDLEIGDDACDPKQAVAVANQMSGSGVALVAGHYC
                     SGSSIPASKVYAEGDMVQISPASTNPAFTDDRAGPNIYRVCGRDDQQGGVAGKYLASH
                     FADKNIAIIHDKTAYGKGLADETKKALNGVGKQEALNEAITAGEKDYSALVSKLKQAD
                     IDVLFLGGYHTEAGLIVRQMRDQGLDTILMGGDALITQEYWSITGPAGEGTLMTFSPD
                     PRKNPAAAEVVKRFKDKGIEPEGYVLYTYAALQAWKQAVEKAKSTASADVVKALNDTE
                     FETVIGKFKFNEKGDPNLPPYAVYRWSNGAYEEIGEEAPAG"
     gene            complement(14834..15046)
                     /locus_tag="VFY74_07560"
     CDS             complement(14834..15046)
                     /locus_tag="VFY74_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931590.1"
                     /translation="MAFEDLKAEIALLFNQMENQPEDRHELYLQIREKLNEMRAFDLP
                     LPDDLVRLEKELEAEFRAEERAAKKR"
     gene            15434..15922
                     /locus_tag="VFY74_07565"
     CDS             15434..15922
                     /locus_tag="VFY74_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931591.1"
                     /translation="MEKLTMDNVVGFVLAILFALVIGISMWALLVGDYVNTVMNEQTR
                     HTLRIVGFTGALAALVTWWVQSFAAKRGFVIRFLFAFFIFVVAFCSFGGLLRFIYIHA
                     TYPNQQDWSLTGMYLASLNDFYTFLLDMLLPPRPAYAALTAAAAIFVALFGPREARTA
                     DI"
     gene            16019..>16743
                     /locus_tag="VFY74_07570"
     CDS             16019..>16743
                     /locus_tag="VFY74_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007629258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5931592.1"
                     /translation="MIPRYSRPEMAQIWEPETRFRIWLKIETYAAEAMASLGLIPVHA
                     ADAVRKRGGFDIDRIEKIEREVKHDVIAFLTSVAEHVGPDARFLHAGLTSSDVLDTCF
                     NVQLVQAADILIADVDALLAALKARAFEYKDTPTVGRSHGVHAEPTTFGLKLAVAYAE
                     FARARERLNRAREEVATCAISGAVGTFAHVDPKVEEFVAKKLGLKVEPISTQVVPRDR
                     HAAYFAALAVVASSVERLATEIR"
BASE COUNT         2790 a         5462 c         5383 g         3108 t
ORIGIN      
        1 gatccgcgac agcggcacga aggtcatggt gacgctcgac cttgcgctgc tgttcgagaa
       61 ggtcgaggcc atgctcgcgc gcgggattct tgaaaaagcc gtggtcgcgc gcttcccctc
      121 gctgctacct cccttgaaat cggccggctt caagttgctg caacgcacga agctcgccaa
      181 cccagcggcg tcaagcgcca gggatcggat cgtgccggaa cggaccgtgc tcgccaatga
      241 cggccgatat cagcggcccc cgatcacgcc ggacgcgatc gcggtcttgc aatacaccgg
      301 cggcacgacg gggacgccga agggagcgat gctcagccat gccaacctta cggtcaatgt
      361 cggccaggtg atcgcctggc ggaaccgcca agcaaccgag gcagaccgca tcctcggcat
      421 cctgccgctc ttccacgtct tcgccatgac cacggtgatg aattacggga tcgccggtgg
      481 catggaactg atcctcctgc ccaagttcga gctcgtcccg acgctcaagc tgatcggcaa
      541 actccgcccg accatgatgc ccggggtgcc gacgctctat aacgccatgc tgcggcaccc
      601 gaagatcgcc aagttcgatc tctcctcgct cgaattctgc atctccggcg gcgcggcgtt
      661 gccggtcgag gtgaaacgcg agttcgaggc gatggcgcgt tgcggcctgg tggaaggcta
      721 cggcctgagc gaaaccgcgc cggtcgcgac ctgcaatccc gtccggaacg gaaaggaagg
      781 gtcgatcggt ctgccccttc ccgccaccga aatctccatt cgctcgctcg aagatccaac
      841 acaggagatg ccgcgcggag agcccgggga aatctgcatc gcgggtccgc aggtgatgtc
      901 cggctattgg aacaagccgc aagagaccga ggcggcgttc gtcgggcgtt acttccgaag
      961 cggcgacgtg ggctacatgg acgaggacgg ctacatcttc atcgtcgacc gcattaagga
     1021 catgatcaat tgctcgggct tcaaggtcta tccgaggcgg gtcgaggacg cactctacgc
     1081 gcatcgcgcc gtcgccgaag tgaccgttgt cgggattccc gatcactatc gcggcgaagc
     1141 gccgaaggcc tacgtcaagc tgaaggaagg catggcggca acggcagcgg aactgcacga
     1201 gttcctgaga gaaaagctgt cgaagttcga gctgccggct gaaatcgagt tccgcgacga
     1261 gctgcccaag accatggtcg gcaagctgtc gaagaaagag ctccgcgccg aagggcagcg
     1321 ccaatcgcgc taattagtcg gcacttggac ggccgaggct gtaaggcgcc gccttaccgc
     1381 caccagggca ggaggggctc ctgtatcccc atctcgcgct gctgtcgccg caactgacga
     1441 cgctctctcc gcgtcagggg gaccggctgg gcgagggtcg catcggcggt ctcggccggc
     1501 gccgtatcgt cgctcgcggc ctccgcttcg gcggcgtctc tatcgtcagg cgcgggcgtg
     1561 gcttgctcgg tggattttgc cgcgggctcc gctttcggcg acggcttcgc cgctgttgcg
     1621 actggcttgg cagcggtggc gacaggcttt ggggtagcga ccggcttggc ggcggcggtg
     1681 gcaaccggct tcggctgggc ctttggcttc gcaggcaccg cagcgctctt cgccgcgggc
     1741 tgagccgtcg agggggcggc agcttccgtt gcgatcggct ttgccatgag catcatgctc
     1801 gggacgttct ctcccgacag ccgcgccgtg gtatcggcat caggcgagac ggcgaggaag
     1861 ccatatccgc gcccggcatc gatttctgtg ggggactcgg tgggggacgc gatctcttgc
     1921 gtgacaggcg tcaccggttc aggcgccacc gccggtgcct ccgtcaagga cggttcggcg
     1981 ggcagttgtg gcgtcgtcat ctgcggcgtg gtcatcgcct gcatctcttg ctgcgcttcg
     2041 gcggcgccga tcgacgcttt ttcgaccgtc gcgtccgcgc tcgggacgga agcgggctct
     2101 gccggcaccg cggccgcggc agaaggcggt gctgcctccg gtagttgcgg cgtcaccgac
     2161 gcctcgatct gctgcgatcc gtccgcaggc gcctccggag tgacctccgc tggcggcgga
     2221 gacgtgcggt accatgttct ctcgccgcag agcagccacg ccttgcagcc cttgagcagg
     2281 agctgcttgc gcgacacgac ggtcagcgtc gcgctgtagg tctgcccgtc ctcgggattg
     2341 tagatttttc cggtccaggt cgaaggcgcg ctgggaacaa gctggctgaa gatcggcaat
     2401 cccatgatct gccggccgcg ctgcgaggga ttctgattgc gaacatcgtg caggggctgt
     2461 cccttgcggt cgttcgggtc cttgagccag gcgatcttgg cgcagagatt ggtccgcttc
     2521 gttccgcatt tgaaaacgtg gatcttggac tcgcgctcgg cgtccggctt catccaatag
     2581 ccggtgggat cggccgaggc ggcgtgggac tggctgacgg gagagaagag agcagcggtc
     2641 aagagcccga tcaccgcagc acacgcatga tgcttcataa gcacctccaa aatcgtcgcg
     2701 cctggcacgt ttcgcgccac ggcaatcact cggttacgtc ctttccccct gtcccttgcc
     2761 gaaccctgcg aggcctctgt ctcacccgat ggcgcgtttg atccgcgctc attgtctcgt
     2821 tcggccggct atgcggcgcc ggctcccgtc cgtgtttcca ccccttccac gatggccgcg
     2881 acacccatgc cgccggccgt gcacaccgat attaaacctc tccggccgcc ctcgctatgc
     2941 aggagcttgg ccaagaccgc cacgattcgc gctcctgtgg ccgcaaaagg atgcccgaaa
     3001 gctattgatg agcccttgac gttgagcttc gcgaggtcga tcgaccccag ggcttcgctc
     3061 cgccccagac ggcgacggca atagtcctcg gattcccatg ctcttagagt cgccagcacc
     3121 tgcgccgcaa aagcctcatg aatctcataa taatcaaagt cctgcagcgc aaggccggtt
     3181 tgctggagga ggtcgctcac ggcaacggtc ggcgccatca gaagtccttc accttgaacg
     3241 aaatctacgg cagaagtgcg gcccaggccg agataggcct gcaccgggat tccccgcttt
     3301 tccgcccact cctccgaggc cagaaggacg gcggcggcgc cgtcggtgag cggggtggag
     3361 ttggctgcgg tgatcgtgcc cgcccccgag cggtcgaaaa cgggcttcag ctcggccatt
     3421 ttctcgagcg aaatgtcctc gcggatattg ttgtcccgga acacgccggc gaagggaatc
     3481 acgaggtcgt ccatgaagcc ctcgtcgtag gcggcggccg ccttcttgtg gctttcatag
     3541 gccagtcgat cctgggcctc gcggctgatg ccccattcct tggccatcag ttcgcaatgc
     3601 tctcccatgc tgaggccggt gcgcggttcg gaggtcgagg gcggctgggg ggcaagctcg
     3661 gcgggggtga agcctttgaa ggaggccgct ttttggccga ggctgcgggc gcgagacatg
     3721 ctcaccaggc gctgggcgaa cttgcgctgg aacacgatcg gcgcgtcgct ggtcgtgtcg
     3781 gagccgaacg cgatgcccga ttcgatctgc ccggtcgcga tcttagcggc aaggccgagc
     3841 gcgccttgga ggctcgtgcc gcaggcctgc atcatggtaa tgccgggggt cgtcggggcg
     3901 agcgcgctgc cgatcaccgc ctcgcgggcg aggttccagt ccttggcgtg agtcacgacg
     3961 gcgccaccca ccacttcgtc gatatgggtg ccgtggagcg cgaagcggtc gatgagaccg
     4021 ccaagcgccg cgacgatcat gtcgagattg gagagctcga cgtaagccgt gtgcgagcgg
     4081 cagaagggag tgcgcgaccc ccccaacacc gcaacgcgcc gcatcgaagc ctcagccatc
     4141 tcgtccctct cggctcccgg tccttgctct aggccgcagc cgccgtcgcc gcccgcgctt
     4201 ccttgcgccg gtagtgcagc ggaccgccgc ggaagggcgc aaaacctgtg ccgaagacga
     4261 cgcccgcatc gacgtgatcg gcgctcgcca ccacacggtc ggaaagcgcg cgctcggcct
     4321 cgtcgagcat cggtttcacc aactcgcgtc cgaggcgctc aagctcggcc tgatcgtagg
     4381 cgacaggttc gcgtttcggc ttgcccccct gccaaagata gaagccctcg cccgttttct
     4441 tgccgagctt gccctgcttg accagagccg ccagaacgtt gtcgccctcg ggcgcaagat
     4501 cgagctcctc gccgacgtga ttggcaatgt cgaggccgac catgtccatg agctcgagcg
     4561 gtcccatcgg catgccgaat ttgagcgcgg cctgatcgat cttctcgcgc ggctcgccca
     4621 attggtagcg gcgcaccgcc tccatcatgt agggcgcaag cacgcggttc acgaggaagc
     4681 cgggacagct cttcacgatc agcggcagct tgttgatggc gttgacgaag gcgcaggcct
     4741 tgcgcacctc ctcttcgcgc gttcgctccc cgcgcacgac ctcgaccagc ggaagctgcg
     4801 ccacgggatt gaagaaatgc agtccgacca gacgccccgg atcggcaaga ggcctcgcga
     4861 tatcctcgag acggagcgat gacgtgttcg tggcgagcac cgccctcggc ttcacctccc
     4921 gctcgagcgc ccgaaacagg ctctgcttga cgtccagctt ctcgacgatc gcctcgatca
     4981 ccacgtcggc ttgcttgatg tgcctgccgg acggatcggc gatgaggcgg gcggccgcgg
     5041 tgtcgatggc gagcggggtg cgaagctgac gcttgaacag gctcttggcg cgggcgagcg
     5101 cctttgcgat ctgcgcttcg tcgagatcct ggaggctcgc ctgcatgccc tggaccacgc
     5161 accacgccgc gatgtcgccg cccatcgtgc cggcgccgat cacatgcacg cgtcgcggct
     5221 tgaatccatt ctttggcgcc tcgtccttca gcatctccga cagcttgaac acgcggcgca
     5281 gattgcgcga ggcttcgctt gccatgagcg gcgtgaacat ctcggtctcg gcgatgcgca
     5341 tgcgcgcggg gtcgtcgccg tagcgctcga acagatcgat gaggcggaat ggcgccgggt
     5401 aatgctcctc gcggaccttc gcggcggtct tggcgcgcat ttgcctggcg agaagcccgc
     5461 gcgccggctg cttgagcatg agccgcttcc accacggcgc gccccgcgac ttgcgcttct
     5521 ggagcacggc cttgcgcgcg gcccagcgga gattgtgacg ggtcgccacg agttgatcga
     5581 cgagacccat ggccctggcc gccgtggggc gcagcatccg acccgtcagc atggcggtca
     5641 tggcgtccag gggtcccgcg gcctcgatcg cgcgcaccgt gccgttgagc cccgggaaaa
     5701 tgccgagctt cacttcggga aagccgagcc gcgtcgcctc ctcgcggtcg gcgatgcgcc
     5761 agtcgcaggc gagcgcaagc tcgagcccgc cgccgaggca atagccgtga atggccgcga
     5821 ccaccgtcac gggaagccgg tcgatccggt tgagcagctc gagcgtctgt ttcaccacat
     5881 cttcgatctt ctgctcggtg tcgaagtttt cgaattccct gatgtcggcg ccggcaatga
     5941 agcttcgctc cttggcgctc atcagcacga gacccttcac ctcgcctgac gcagccgcct
     6001 gctcgaccgc catgacgatc tcgccgagct cctcggtcgc ccggcggctg agcgagttca
     6061 tgctttcgcc ctggcgatcc atcaccgccc aggcgatgcc ttcgaaatcg acggtgaaac
     6121 gccaatcctg caaatcgggg agtgtcgtcg gcatggtcgg acgctactcg gcggcaatat
     6181 tctcgggtcg ggtggcgctg tcctgaggcg gcggcgcggg cgaccggccc cgcgcaagct
     6241 cgctcgccgc gaaatcgtca acggcgatgg cccgggccac gagatcgcga agctcggcaa
     6301 gggcgcgcgc ttcggtcgcg ctgagcacgc ctttcgattc cgcttcggca atccagtcgt
     6361 ggtcgtggaa acgccgcacc tcgcccttgc ggatcgcccg ctcgagcttc ctgtcggcct
     6421 cctcgcaggc gaccgccttg gcaagcgtat agtcgagcaa cccgacccgg tctttcggat
     6481 cgtcggtcgc gtagatgtgg cgtgtgaggc gatcgcggaa cgcgccaggc tcgagtgccg
     6541 cgcgcacgat ttgcttgccg gcttcgtcgc tcgccgggcg ccgtcgcaga ccgaacggaa
     6601 agacgagcgg cgccatcgcc caggccgccc acctgaccgg aaaattgcgc aaggtgccga
     6661 ccagcgcctg atcgaagcgg tagaggcagt ttctcgcgca atactccacg accttgaggt
     6721 cctccttggg gcggccgtcg tcgtcatagc gcttgagcac agaggagagg agatagagct
     6781 cggcgagcgc gtcggccatg cgcccggtga tcttctgctt caccttcagc ccgccgccga
     6841 ggagcgccac cgtcgcgtcg gcgacgagcg caaaggagcg cgacgcacgc gcaagctcgc
     6901 gccacaaggc cctcgtgtcg ccggcattcg gcggcgccga ggcgaaaagg ccgaaggtca
     6961 ggttatggaa cagcgcgccg gcggcattcg agaccgtgaa agcgacgtgg tcctcgaagg
     7021 cgcgcgcgaa gcgctcagtg ccgcgtgcgc gatcggggtc ttgaagcgcc tcgatctcgg
     7081 tgaagagata ggggtgactg cgcagcgcgc cttgagcgaa ggtgatcagc gtgcgcgtga
     7141 ggatgttggc gccttcgacg gtgatggtga ccggcgtcat ttgataagcg ccctgaaggt
     7201 agttgctcgg cccatcgcag atgcccttgc cgccatggat atcgagcgca tcgttcacgc
     7261 tctgacgcat gcgctcggtc gagacgtatt tgagcagcgc cgagagcacg gcgggcttct
     7321 cgccggccga gaccatcgag gcggtcagcg cccgcgcggc ctcgagcgcg tatgccgtct
     7381 cgatcatgcg cgcaagcggc tcctccacgc cctccatgta gccaatcggc aaattgaact
     7441 gctttctgat gcgtgcgtag gctgtgctgg cgcggagcag ggcttttgcc gcggcggcgc
     7501 tcgtcgcggg aagcgagatg gagcgtcccg ctgcgaggca gctcatcagc atgcgccacc
     7561 cctgcccggc cctctcctcg ccgccgatga tccaatcgat cggaacgaac acgtcgcgtc
     7621 cgcgcgttgg gccgttggga aagcctgtgc ccccgggaag atgccgccgt ccgatctcga
     7681 cgccgggatg gttcgtggga atgagcgcaa gcgtaatgcc gacatcctcg ccgcggccga
     7741 gatgcttctc cggatcgaac aggcggaacg caaggccgag cagcgtcgcg ttgggcgcaa
     7801 gcgtaatgta gcgcttgtcc caggtgagcc tgatgccaag agtgtccctg ccctcatgct
     7861 ctccgcgcgt gacgacgccg atgtcctgca tggcggcggc gtcggagccg gagaacggcc
     7921 cggtcagcgc gaagcaaggg atctcctcgc ctctggcgag acgcggcagg aaatgttcct
     7981 tctgcgcatc ggtgccgtat ttctcgatca gctcgccggg gccgagcgaa ttcggaacca
     8041 tgacgatggt gacgccgccg gggctccgcg atgaaatctg gccgaggatc agcgactgcg
     8101 cctgggcgga gaagccgaga ccgccgtgct ccttggagat gagcatgccg agaaagccgc
     8161 gttcgcagac gaagcgccag atgtcggcgg gaatgtcgtg cagttcgtga cggatgcgcc
     8221 aatcgtcgag cttcctgcag agctcggcgg tcggcccgtc gaggaaggcg cgctcctcgg
     8281 cggtgagcgt gaccggcgcg acgcggcgaa gcttttccca atccggcgtt ccggagaaga
     8341 gctcggcgtc ccaaccgaca gtacccgctt cgagcgcctc gcgctcggtc tccgagatcg
     8401 caggcatcac gcccttgagc gcgcgatagg cgggcatcac gatatagttg cgcttgatgt
     8461 ccggaaggct tgcaaggaac agaagccccg cggccagcca gccgaacagg gaccaccacg
     8521 taaagaccgg ccaatggaaa tggccgccgg cgagccccat ctgcgcgccc aaggtgaaaa
     8581 gagcgacggc aagcgcccaa accctgagcg aggcgcggtt catggccaga gcgaacgccg
     8641 ccaggatgaa aacgagcacg aaaatgactt cagccatgag cggcctctag attcgtcttg
     8701 gcccccgctc gtgccccact gaagcgccct gtgcgggcca gccgttccgc cggtgcggcg
     8761 cgcacggggt cggctttttg ccctctatgg tctggatggg gcatcgactc aagctctttc
     8821 ctgccctctc ggcgcgacag ttcggccctg tcgaatcggg cagcccatcc tgccgccaaa
     8881 cagattccta gtgaattaac ggtccggcct ccttctatgt gcttgggccg cgccgaggtt
     8941 ttcttgtgcc gggggggcga attggctaag caagtcatca aaacgcggcc gccgaaggcc
     9001 cgctgggcaa ggacaccgca atggcagagc cggagcgacc gcagctaaac ccctatcagg
     9061 tcgagcttga ggccggcagg cgctattcct ggtgccgttg cgggcggagc aagaggcagc
     9121 ctttctgcga cggatcgcac gcggggacgg ggatcgaacc cctgacgttc acggccgagc
     9181 gcaccgaact cgccttgctt tgcggctgca aggatacagg cgatccgccc tattgtgacg
     9241 gctcgcattt gttgctctga tcttgccctc tacaagattg gcggcgttgt gcgcggcgcg
     9301 cccttcttgc taagaagggg ccgcaaaagg ggccgggcct ggcggctgac gttcgggccg
     9361 cccgtgcaag ggggacgccg ggaccaatgg cctatttcct gcagcagctg gtcaacggga
     9421 tcgcgctcgg ctcgatctat gggctgatcg ccatcggcta caccatggtc tatggcatcg
     9481 tcggcatgat caatttcgcc cacggcgaca ttttcatggt gggcgccttc atcgcgctga
     9541 tcctgctctt ggcgttgctg agtgtgggcg tgaccatgat cccgctcgcc ctcctcatcg
     9601 tgctgctcct tgccatggcc ctcacggcgg tatggggctg gacggtcgaa cgcatcgctt
     9661 acaggccctt gcgcgagtcg tttcgcctgg ccccgctcat cacggccatt ggcatatcga
     9721 tcatattgca gaatttcatt caggtggcgc aaggcgcgcg cgtgaaaccg ctccagcccg
     9781 tcgtcaccgg aggccaccgg gtgttcgagg cgggcggctt cgccgtcagc atctccaata
     9841 ttcagatcct catcgtggtc acgacgctcg tgctgatgat cgtgttctgg ctcatcatca
     9901 cccgcacggc gctcggccgg gcgcagcgcg cctgcgagca ggatcggatg atggcctcgc
     9961 tgtcgggcgt caatgtcgac cgcaccatct cgctcacgtt cgtgctcggt gcggcgttgg
    10021 ccgccgtggc tggactgctc tacctcctct attacggcgt gatcgacttc tacatcggct
    10081 tcgtcgccgg catcaaagcc ttcaccgcgg cggttttcgg cggcatcggg tcgctgccgg
    10141 gcgccatgct cggcggactg atcatcggcc tgatcgagac gttctggtcg gcttatttca
    10201 ccatcgagta caaggacgtc gccgccttct cgatgcttgc catcgtgctg atcttcttcc
    10261 cgaccggcct gctcggccga cccgaagtcg agaaggtcta gcatcttggt tgggcccgat
    10321 aagtccttca gtcaccggct caagtcggcg cttctgaatg cgaccctctc cgcattggtc
    10381 gcgtttctcc tgttctgcct gatccttggc gtccgcaccg tcgatacggt gcaagggctc
    10441 accttgagac ctcgcctcct gcttctcgcc gtcgcggtcg ggatcgtctt tgtcggcagg
    10501 ctggcgctcg acctgttctg gtggaaccgg aactggaact tgcccaagcc cgtagggctc
    10561 aagaggctca gcgacgcgac cgctcagtat ggcggcatcg cgctcatcgt cacggcggtt
    10621 cttctcccca tcgtcgcact ggccatcgag aagtccctgc atctcggcct gccgctccgt
    10681 tacctgttcg atctttcgat cgtcgttctc acctacctca tgctcgcgtc gggcctcaat
    10741 atcgtggtgg ggctcgccgg gctgctcgac cttggctatg tcgcgttcta cgcggtcggg
    10801 gcctacagct ttgcgcttct tgcgacgaat ttcggctggg gcttctggat gtgccttccc
    10861 ttcgcgggat tgtttgcagc gctctggggc tgcactgtcg gccttcccgt gctccggctc
    10921 agaggcgact atctcgccat cgtcacgctc gccttcgccg agatcgtgcg catcgtcttg
    10981 ctcaattgga ccgagctgac caacgggccg aacgggattt ccggcatccc cgatcccacc
    11041 ctgttcggtc ttgcgttcac gccccgcggg gatgtcacgt tcagcagctt cttcggcatc
    11101 agatatgagc cgctccaggg aatcgtcttc ctctattacg tgatcctcgc gctttccctg
    11161 ctcaccattt tcgtcacgat gcgcttgcgg cgtttgccag tcgggcgcgc ctgggaggcc
    11221 atgcgcgaag acgagatcgc ttgccgctcg ctcggcatca acaccaccgt caccaagctt
    11281 tccgcgtttt ccaccggggc catgttcggc ggtttcgccg gcgccttctt cgcgacgcgc
    11341 caaggtttca tcagcccgga gtcgttcacc ttcatcgagt cggcgacgat tctcgccatc
    11401 gtcgtgcttg gcggcctcgg cagccagctc ggcgttgcgc tcgcgtcgat cgcgttgatc
    11461 ggcggtttcg aggcgctccg tcatacccac ggactgaagg tcatcttcgg tccggatttc
    11521 gaccccgcgc tttaccgaat gcttctgttc ggactggcga tggtcatcat catgctgtgg
    11581 cgcccgcgcg gaatcgtggc gtcgcgaacg ccctcgatcc tgcttgagcc gcaacgcggg
    11641 ggcgtttctc tggcgaagga acgccgggca tgagggggtg gcagaaggat ccgatcctca
    11701 gggtcgaaca cctgaccatg cgctttggcg gcctgatggc ggtcgacgac gtctccttcg
    11761 aggccggccg cggcgacatc acggcattga tcggtccgaa tggcgccggc aagaccaccg
    11821 tgttcaactg catcaccggc ttctacaagc cgaccgaagg gatgatcacc ctcacccatc
    11881 ccaatggttc gagcttttat ctcgagcgcc tcgacgatca cgacatcaac tcaagagccc
    11941 gggtcgcccg cacgttccag aacatcaggc tgttcaaggg gatgacggtg ctcgagaatc
    12001 tgatggtggc gcagcatcag acgctcaatc gcgcctcggg cctcacgttt ggcgcacttc
    12061 tcggcgtcgg cggctatcgc gccgccgaaa aggatgccat cgcgtttgcg cgagcgtggc
    12121 tcagccgcgt gggtctcacg tcccgcgccg acacgccggc gggcgacctt ccctacggcg
    12181 accagaggcg gcttgagatt gcgcgggcca tgtgcacgcg accgcttctg ctttgcctcg
    12241 acgagccggc tgccggattg aacgcgcgcg agagcgaggg gctgaacgag cttctctatt
    12301 cgatccgcaa acaagacggc atcgccattc ttctcatcga gcacgatatg agcgtggtca
    12361 tgcgcatctc cgaccacgtg gtcgttctcg attacggcaa ggagatcgcc gacgggacgg
    12421 ccgaagaggt gcgcaacgac ccgcgcgtaa tcgccgccta tctgggtgtc ccggagaaag
    12481 agctcgccaa ggtcgagcgg gaggttggcc tgtgagcgcc cccctgcttg cgatcggcaa
    12541 cgtcaccgcc ggctacggca acatccccgt cctgcacggg gtgagcctcg acatctcgaa
    12601 gggcgagatc gttacgctca tcggcgcgaa cggcgccggc aagaccacgc tgatgatgac
    12661 catcttcggt cgcccgcgcg cggcccaagg cagaattctg ttcgaaggcg aggacatcac
    12721 gcatctgccg acgcacagaa tcgcgcgatt gtgcatcgcg cagtcgccgg aaggacggcg
    12781 catcttcccg cggctgacgg tcctcgaaaa cctgcaggtg ggcgccctca tcgccaatga
    12841 tcccacggcc ttcgagaacg atctcgagcg ggtttgcgcg ctcttcccgg tgctgaagca
    12901 gcgcaaggcg caacgcggcg gcacgctgtc gggcggcgag cagcaaatgc tcgcaattgc
    12961 ccgagcgttg atgggtcggc ccaagttgct gctgttggac gagccgtcgc tcggacttgc
    13021 gccactcatc gtgaagcaaa tcttcgagac gatcggggag ctcaaccgac aaggcttgac
    13081 agtcttcctc gtcgagcaga atgcctatca cgcgctgaag ctcgcccatc gcggctacgt
    13141 catggtgaac ggcaaaatca cgatggccgg cacggggcgc gagctgctcg aagccgaaga
    13201 ggtgaaggcg gcctatctcg agggcggcgc ccatggcgcc ggtcaggcac ggacatgagc
    13261 tggctttccg acgacggact tcgcgtgttc ctcgtgctca ccgtcatcat cggcggcggc
    13321 gcggcgttcc tcggcgggcg cggcctggcc cggtcgtgga agccgttttg gcgcttgttc
    13381 gtctatatgg cgctgctggc cgcggcgatc cgcttcttcc attacgcttt gttcgacggc
    13441 gcgctcctct caccctatta ctatctcgtc acctatgccg tgcttctcgc cgccgcatgc
    13501 ctcgggtttc ggacgatgcg gacaacgcag atggtgacgc aatatggctg gctttaccag
    13561 cgcaccagcc cgctcacctg gcgcgaccgg cgcgcgtgag cgatctcacc ttggcgcgcc
    13621 ataattagcg atctcacctt ggcgcgccat aattgatgta gattggattg gatcgtatag
    13681 agagggaggt agcaatgaaa cgcatggccg ggctgatcgc agccctcgct ttcgccgtac
    13741 tggccgttgg ctgcggcgag gacagcaata ccacgccgat cgccatggtc ggtcccgtga
    13801 cgggtcaata cgcctccttc ggcgcgcaga tgcagaacgg cgccgagctc gccgtcgagg
    13861 acatcaacgc cgcgggcggc gtgctcggca agaagctcga tctcgaaatc ggcgacgacg
    13921 cctgcgatcc gaagcaggcg gtggcggtcg ccaatcagat gagcggcagc ggggtcgccc
    13981 tcgtcgccgg ccattattgc tcggggtcct cgatcccggc gtcgaaggtc tacgccgagg
    14041 gcgacatggt gcagatctcg ccggcttcca ccaatcccgc tttcaccgac gatcgcgccg
    14101 gacccaatat ctatcgtgtg tgcggccgcg acgatcagca gggcggagtc gcgggcaagt
    14161 atctggcgag ccacttcgcc gataagaaca tcgccatcat ccatgacaag accgcgtacg
    14221 gcaaaggact cgccgacgag accaagaagg cgctcaacgg cgtcggcaag caggaagcgc
    14281 tcaatgaggc catcaccgcc ggcgagaagg actactccgc gctggtgtcg aagctgaagc
    14341 aggccgatat cgatgtgctg ttcctgggcg gttaccacac cgaggccggt ttgatcgtgc
    14401 ggcagatgcg cgatcagggc ttggacacga tcctgatggg cggcgacgcg ctcattaccc
    14461 aggaatattg gtcgatcacg ggaccggccg gcgaaggcac gctgatgacc ttctcgcccg
    14521 atccgcgcaa gaacccggcc gccgccgaag tggtcaagcg cttcaaggac aagggcatcg
    14581 agcccgaggg ctatgtgctc tacacctacg ccgcgctgca ggcctggaag caggcggtcg
    14641 aaaaggcaaa gtcgacggct tcagccgacg tcgtgaaggc gctcaacgac accgagttcg
    14701 aaaccgtgat cggcaagttc aagttcaacg agaaaggcga tccgaacctt ccgccttatg
    14761 ccgtctatcg ctggtcgaac ggggcctatg aggagatcgg cgaggaggcg cctgcgggct
    14821 aaagggtcgg agcctagcgc ttcttggccg cgcgctcctc ggccctgaat tcggcctcga
    14881 gctccttctc gagccgcacc agatcgtcgg ggagcggaag gtcgaaggcg cgcatttcgt
    14941 tgagcttctc cctgatctgc aggtagagct cgtgccggtc ctccggctgg ttctccatct
    15001 gattgaaaag aagcgcgatt tccgccttca aatcctcgaa tgccatggcg tgggcgcctc
    15061 cgtgtcgggg gccgggatcg gaccacaaac ggcccagggc ggcaagtacg ctcgctaacc
    15121 cctctccctc gtgggagagg gtgcacgcca tcgcccgaag ggcgaaggcg ggcgggtgag
    15181 ggggtcactt ctggcgcctc gctggacccc cctcaccgcg ccgcacgcgg cgcgcgctct
    15241 ccccaagggg agagcgggac aacgcgtcat cctgaggagc gagcagcgag cctcgaagga
    15301 tgaaagttgg ttgcgtcctt cgagggccgc ctgcggcggc ctcctcagga tgacgaaggg
    15361 tgtcgattgc gaggggccga tttcccctgc atagtcaagc ataactaagg atcaccaacg
    15421 ggcgggacgc gaaatggaaa aactgacgat ggacaacgtg gtgggcttcg tgctcgccat
    15481 cctttttgcg ctcgtgatcg gcatttccat gtgggcgctt ctggtgggcg actacgtgaa
    15541 cacggtgatg aacgagcaaa cccggcacac gctgcgcatc gtcgggttca ccggcgcgct
    15601 cgcggcgctc gtgacctggt gggtgcagag ctttgccgcc aagcggggct tcgtgatccg
    15661 cttcctgttc gccttcttca ttttcgtggt ggcgttttgc agtttcggcg gcttgctccg
    15721 cttcatctac attcacgcca cctatccgaa ccagcaggat tggtcgctga ccggcatgta
    15781 tttggcctcg ctcaacgact tctacacatt cttgctggat atgttactgc cgccccgccc
    15841 ggcttacgcc gctctgaccg cggctgccgc gatcttcgtc gctctgttcg gaccgcgcga
    15901 agccaggaca gccgacatct gatcgccggc cccgacggaa gcggcgccgg gccctttgct
    15961 tgaggttaac gcctgttccg gcatcttgct cgcggacagg ccgctttgga aaccatgaat
    16021 gattccccgc tatagccgcc ccgagatggc ccagatatgg gagcccgaaa cccggttccg
    16081 catctggctc aagatcgaga cctatgccgc cgaagccatg gccagcctcg gcctgatccc
    16141 ggtgcatgcg gccgacgcgg tacgaaagcg cggcggcttc gacatcgacc gcatcgagaa
    16201 gatcgagcgg gaggtgaagc acgacgtgat cgcctttctc acttcggtcg ccgagcatgt
    16261 cgggcccgat gcgcgcttcc ttcatgccgg gctcacctcc tccgacgtgc tcgacacctg
    16321 cttcaacgtg cagctggtcc aggcggcgga cattctgatc gccgacgtcg atgcgctgct
    16381 tgccgcgctg aaagcccgcg cgttcgaata caaggacacg ccgaccgtcg gccgcagcca
    16441 cggcgtccat gccgagccga ccacgttcgg gctcaagctt gccgtcgcct atgccgagtt
    16501 cgcgcgcgcg cgggaacggc tcaatcgcgc ccgcgaggag gtcgcgacct gcgccatctc
    16561 cggcgccgtc ggcaccttcg cccatgtcga tccgaaggtg gaagagttcg tggcgaagaa
    16621 gctcggcctc aaagtcgagc cgatctcgac acaggtcgtg ccgcgcgacc ggcatgccgc
    16681 ctacttcgcc gcgctcgccg tggtggcaag ctcggtcgag cggctggcga ccgaaatcag
    16741 gca
//
LOCUS       DASUQP010000172         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1040299, whole genome shotgun sequence.
ACCESSION   DASUQP010000172 DASUQP010000000
VERSION     DASUQP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040299"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFY74_07575"
     CDS             <1..404
                     /locus_tag="VFY74_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5931593.1"
                     /translation="PHNALGLVRINMPNKHSVYMHDTPLKQLFSQSARAFSSGCVRVQ
                     KVLELVAWLLGEQSGWTLERVEAQIASGEKRDVKLPQPVPVHFVYVTAFASGNGVAQF
                     RPDIYGRDATFGAPDDPQDAVVAQQARAVTP"
     gene            556..1317
                     /locus_tag="VFY74_07580"
     CDS             556..1317
                     /locus_tag="VFY74_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5931594.1"
                     /translation="MPISVVIPAFNEEGNIGRLIGETFATVPKEALGEVIAIDDGSDD
                     GTAAEIKALLSQHPRLRYVRHAHRAGQSAALYIGVRAARFPVIATLDGDGQNDPADIM
                     PLFRLLGGQGREPAMAAGIRANRKGSGSRKAASRLANFLRDKVLADGCPDTGCGIKLF
                     RREAFLALPYFVGMHRYLPALFLSYGHEIAYETVNDRPRLKGKSKYTNLGRALIGLYD
                     LLGVSWLRKRTLIPPIAEDVSGESPAKRGEQGDRI"
     gene            1363..1689
                     /locus_tag="VFY74_07585"
     CDS             1363..1689
                     /locus_tag="VFY74_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5931595.1"
                     /translation="MSGGALVHWWLTTPTTELIWVGIGLSAQLLFSMRFLVQWIATEK
                     ARTSIIPEAFWYFSFCGGVMLLAYACYRVDPVFIIGQATGLVIYTRNLYFIWLGKRAP
                     SPAKLA"
     gene            1751..2500
                     /locus_tag="VFY74_07590"
     CDS             1751..2500
                     /locus_tag="VFY74_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5931596.1"
                     /translation="MRLKDLTLFERILLALSLIFLSLLIIDPFMYERALALPRGARKF
                     FQAVTDIGKSNWMLIPTGVAIALALVLQKRYGGWRNSAAYGLIASTIGFVFVSIGGAG
                     LIANLAKNILGRARPKLFEIVGPLDFQLFAFDPAHASLPSGHATNIFAFATVIGMLWP
                     RGRVLLYTIAAWIAASRVLIGEHYVTDVVLGAILGTAFPYYVRDRFAARRWLFEPAPD
                     GGYRLRGLRTQRWLGWPDMRYRAPEISNRPG"
     gene            2644..2720
                     /locus_tag="VFY74_07595"
     tRNA            2644..2720
                     /locus_tag="VFY74_07595"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2678..2680,aa:Met,seq:cat)
BASE COUNT          481 a          895 c          877 g          547 t
ORIGIN      
        1 gaccgcacaa cgcgctcggg ctcgtgcgca tcaacatgcc caacaagcac tccgtctata
       61 tgcatgacac gccgttgaag cagcttttca gccagagtgc gcgcgccttc tcatcaggct
      121 gcgtgcgggt gcagaaggtg ctcgagctcg ttgcctggct gctcggcgag cagagcggat
      181 ggactttgga acgggtcgag gcgcagatcg cgagcggcga gaaacgcgac gtgaagctcc
      241 ctcagcccgt gccggtgcac ttcgtctatg tgacggcgtt cgccagcggg aacggcgtgg
      301 cgcagttcag acccgacatc tacgggcgcg atgcgacctt tggcgctccc gacgatccgc
      361 aggatgcggt cgttgcccag caggcccgcg cggtcacgcc ctagccgccg cttccctggc
      421 acgcccggcg ctacgattgc aaagctcgac gtaacacgac atcgcggcag cgcgcgtgta
      481 caatgtggtt gacgccgagg aacggtccgt cccgcccgcg tgctttctgc cgcgcttcgc
      541 aaagagcgag cgttcatgcc catctccgtc gtgatcccgg cctttaacga ggaaggcaat
      601 atcgggcgcc tgatcggcga gacctttgcc acagtgccga aggaggcgct cggcgaggtg
      661 attgccatcg acgacgggag cgacgacggg acggcagccg aaatcaaggc gctgctcagc
      721 cagcatccgc gcttgcgcta tgtgaggcac gcccacagag cggggcaaag cgcggcactc
      781 tatattggcg tgcgcgccgc ccgtttcccg gtcatcgcca ccctggacgg cgacgggcag
      841 aacgacccgg ccgatatcat gccccttttc cgcctgctcg gcggccaggg ccgtgagccc
      901 gccatggcgg cggggatcag ggcgaaccgc aagggatcgg gttcgcgcaa ggccgcgtca
      961 cgccttgcca atttcctccg tgacaaggtg ctcgccgacg gctgccccga tacggggtgc
     1021 ggaatcaagc ttttccgccg cgaagccttc ctcgcgctgc cctacttcgt tggaatgcat
     1081 cgctatttac cggccctgtt cctgagctat ggtcacgaaa tcgcctatga gacagtcaac
     1141 gatcggccgc ggctcaaggg caagtccaag tacacaaatc ttggccgcgc gctgattggg
     1201 ctgtacgatc ttctcggcgt cagttggctg cgcaagcgga cgctcattcc gccgatcgcg
     1261 gaagacgtct cgggcgaaag cccggcaaag cggggagagc agggcgatcg catctgatcg
     1321 cgtgctgcgc cgacgggagt agtccacggg ggagtcattg ccatgtccgg aggggccctt
     1381 gttcactggt ggcttacgac gccgacgacg gagctgatct gggtcggcat cggcctgtcc
     1441 gctcagcttc tgttctcgat gcggtttctg gtgcaatgga ttgcgacgga aaaggcgcgc
     1501 acaagcatca tcccggaggc gttttggtat ttcagcttct gcggcggggt catgctgctc
     1561 gcttacgcct gctaccgggt cgaccccgtg ttcattatcg gccaagcgac cggtctcgtg
     1621 atctatacgc gcaatctcta tttcatctgg cttggcaagc gtgccccaag cccggctaag
     1681 ctcgcctagc cggatcgcgc gttcgtcggc gcgcatgtcg ttggaaatgg cgtgaggcga
     1741 gaggctcgcg gtgcggctga aggatctcac cctcttcgag cggattttgc ttgcgctttc
     1801 gctcatcttc ctcagcttgc tcatcatcga tcccttcatg tacgagcggg ccttggcgct
     1861 gcctcgaggc gcacgcaagt tctttcaagc cgtcaccgat atcggcaaat cgaactggat
     1921 gctgatcccg accggcgtgg ccatcgcgct cgccctcgtg ctgcaaaaac gctatggcgg
     1981 atggaggaac tctgccgcct acggcctgat cgcgagcacg atcggcttcg tgttcgtcag
     2041 catcggtggg gccgggctca tcgccaatct cgccaagaac atcctgggtc gcgcgcgccc
     2101 gaagctgttc gagatcgtcg ggccgctcga cttccaattg ttcgccttcg accccgctca
     2161 cgcctccctg ccatcgggtc acgccaccaa catcttcgcc tttgccactg tgatcggcat
     2221 gctgtggccg cgggggaggg tgctgctgta cacgatcgcc gcttggatcg ccgcgagccg
     2281 ggtcctgatc ggtgagcact acgtcaccga cgtggtgctc ggcgcgatcc tcggaacggc
     2341 gtttccctat tacgtgcgcg atcggttcgc ggcgcgccgc tggctgttcg agccggcccc
     2401 cgacggcggc taccggctcc ggggtttgag gacccagcgc tggctcggct ggccggacat
     2461 gcgatacagg gcgccggaaa tttcgaaccg cccgggttga aaaattcatg ccaaagacgg
     2521 aggcaacgcc tatattggag gcgaggcgct gcggccggct gcctgccggt gaagcccttc
     2581 acatctctct cgaacctgct ataggttccg gcccccgtct tcttcggggc gaaagacatt
     2641 gggcgcgggg tggagcagcc cggtagctcg tcaggctcat aacctgaagg tcgtaggttc
     2701 aaatcctacc cccgcaacca acgcataatc cccttgtgac ctttcttgac tcggccgagg
     2761 cagcttggtc gcgaaaaaat tttataaaca cgtaaaatct
//
LOCUS       DASUQP010000173         9889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1177811, whole genome shotgun sequence.
ACCESSION   DASUQP010000173 DASUQP010000000
VERSION     DASUQP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177811"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFY74_07600"
     CDS             complement(<1..452)
                     /locus_tag="VFY74_07600"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5931597.1"
                     /translation="MFTKKPESGESVTKMGERSSELKTPFTERPATYSSKSPSSAASK
                     MVPSIIGEDLSISGNVTSKGEIQVDGEIQGDINCGSLLLGDKSQVIGGVLAEDVVVRG
                     RVVGSIRGLRVTLQAQSHVEGDIFHQSLAIEQGAYFEGKSRRSDDPLA"
     gene            958..4785
                     /locus_tag="VFY74_07605"
     CDS             958..4785
                     /locus_tag="VFY74_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEX5931598.1"
                     /translation="MSEPIAGALARLTTESGRAIQVLRATVRVVTIRVLAQGRRWLRR
                     LDGGSGATNPSISTALVKLSTEGSKAILALPERGRALAKRVPEPVQIELKKLSSRGAH
                     VTREIFAGILVVGLVAIVLGYGRLARGPISLSGLVPTIEDSINGELADLTVKIDDAIL
                     QRASDGPGVLFRLRNIRLVDKNGAILAQAPLAAIGMSGSALLSGRLAPGSVDFIGPRL
                     LLTYEPGQGLSLSFSRLAPSGAEPTIPGVPAPEEATAPADEARPPESVIAKRQVQRQG
                     SGRVLDVTRTVAEVFEKARSGDTSYLTRFGVKDALVVLNHGGSQTSWQVPDFAIDLEH
                     HNQRSILVGQANIASAKGDWQLEMRAAEQTKRQSLSVTALIQNLVPSGIAGNFSSLGL
                     LKALDMVVNGQGTVELSNAGDFVAGEADFDLETGQITPRWDPDSPLRIDGGRVRLSYR
                     KANGVIEIAPSTLVWGQSRATFSGEFRPARDERGTLTAWKFELRANEAVLAVEESGLA
                     PMTVDEWQATGSLFPQDGRISLSRFVIRAGDASIAFSGTAVDTEGAEGVHLAGELSPM
                     PVDVFKRFWPKFLAGNARAWVLDRVSGGQVLGGTFAVNLGPAEFAAMKAGQELSPEAV
                     KVELKLSGLSVAYLPEMPPVVTGESTLTVAGMTFAVDIPEGQITPPSGRTIALSQGRF
                     SIPDLRADPQQGDIVFKAGGETPTVLELLDHEPLGYLKSVGMKPDFLAGTTEGDFFLS
                     MPLLADLEFEQIKVNGSARLNDAIAGTLADNVDVAGGSLDVTLSEQGLEAMGAISIKG
                     VPAELSWQRIFFAPDAEQPPIQVTARLDAATREQLGLKVNHLVQGPTPVTLRVAGLGT
                     ETPTGMTLEADLTEAQLLFGGLGWTKPSGRAATLHLDVLQNEDGSTVLDNLKIVGDDI
                     DIRGGIVLDAEQHLKEFHFSDFSVNQLSHVEITAAVRDDQVLEIKAEGPSYDGRQFFE
                     SLFSAGQLEGSAEPDEPFGVDLTASIGTVVGFYDTTAQNVEVTMKKRNGRIVALDAKA
                     GLNGKAPAAVQLVQKQGARMIEAEARDAGAAFRLVGFYPSVEGGEASLQVNLDAGGAG
                     TKSGTLWARNFVVLGDSVVGEVLTDPNSAAVLGPSERKQQVTRQRIAFSQLRAPFSVG
                     GGKFRLHDAYINGPLLGATMRGTVDFKAEMVELGGTYVPLYGLNSALGSIPLLGRVLV
                     GREGEGVVGITFAIKGKLDDPSVLVNPMSVMTPGIFRQIFEFTGSVPDPNAKPEASSY
                     MVPGQQFGQPAPQ"
     gene            complement(4787..6040)
                     /gene="tyrS"
                     /locus_tag="VFY74_07610"
     CDS             complement(4787..6040)
                     /gene="tyrS"
                     /locus_tag="VFY74_07610"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5931599.1"
                     /translation="MSKLKSDFLQELAARGFIHQLTDEAGLDRLARSERLTGYIGFDC
                     TAPSLHVGHLVSIMMLRKLQQTGHRPIVLIGGGTTKVGDPSGKDEARKLLSETEIAAN
                     TAGMEQVFTKFLSFGSGAVDALMINNADWLDGLNYIDFLRDYGRHFSVNRMLTYDSVK
                     LRLEREQALSFIEFNYMVLQAYDFVELYRRHGCRLQLGGSDQWGNIVSGIELGRRTDG
                     AELFGLTSPLLTTASGAKMGKTAAGAVWLNPDMVSPYDYWQYWRNSEDGDVERFLRLF
                     TELPLDEIARLAKLRGAELNEAKKILATEATALVHGRDAADRAAETARATFEQGEIAV
                     TLPTFDIPRSELEKGWGVLSANVEIGFVASTGEARRQIRSGGIKVNDVAVTDEKRKLT
                     LADLSPEGVIKLSLGKKRHALIRPV"
     gene            complement(6131..6799)
                     /locus_tag="VFY74_07615"
     CDS             complement(6131..6799)
                     /locus_tag="VFY74_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5931600.1"
                     /translation="MPEVNISGPAGRIEARYHHEKGPNAPVALILHPHPQFGGTMNNQ
                     VVYHLYYSFQRRGFSVLRFNFRGVGRSEGLFDNGPGELADAATALDWLQAYNADAATC
                     WIAGVSFGAWIGMQLLMRRPEIEGFISVAPPANLYDFTFLAPCPSSGLMVNGAKDRVV
                     PTESVRGLVERLKTQKGIVIDHATVPDANHFFEGRMDGLMEVVDHYLDKRLGKTKRRK
                     DSAA"
     gene            7081..8223
                     /locus_tag="VFY74_07620"
     CDS             7081..8223
                     /locus_tag="VFY74_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007696253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5931601.1"
                     /translation="MNQRSYLDYNATAPLRDEVREAVVEALSIHGNPSSVHAEGRDAR
                     RAVEAAREKIAALAGGKAEDVVFTSGATEANALALAPRAGARWHCYVSAVEHPSVLSG
                     GRFAAGSVSRLPVTADGCADLGILAEKLAESDRAGVRPFVSLMAANNETGAIQPVAEA
                     ARVVHDAGGVLHTDAVQAAGRIKLDMDALGADMLSLSAHKIGGPKGVGALVLHDGASV
                     EPLIKGGSQERRRRAGTENVAGIVGFGVAAELALADLAKAEKTARLRDELEQGALSLA
                     PDAVVLSGRVDRLPNTSCLAVPGIKAETLVIRLDLAGIAVSAGSACSSGKVEASHVLA
                     AMGLSDELARGGIRVSLGFGTSRNDINTFLKAFGELIGRLRPGVAA"
     gene            8253..9734
                     /gene="sufB"
                     /locus_tag="VFY74_07625"
     CDS             8253..9734
                     /gene="sufB"
                     /locus_tag="VFY74_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5931602.1"
                     /translation="MPAVQETVARVKEIDVDKYKYGFTTDIESVKAPKGLNEDVVRFI
                     SAKKGEPDWLLAWRLEAYRRWLTLEEPTWANVHYPKIDFQELYYYSAPKSTEGPKSLD
                     EVDPELLRTYARLGIPLSEQKVLAGVEGASLEGRVAVDAVFDSVSVVTTFKEELAKAG
                     VIFSSVSEAVKTHPELVRKYLGSVVPITDNFYATLNSAVFSDGSFVYVPQGVRCPMEL
                     STYFRINEKQTGQFERTLIIADKGSYVSYLEGCTAPMRDENQLHAAVVELVALDDAEI
                     KYSTVQNWYPGDKQGKGGIYNFVTKRGDCRGHHSKISWTQVETGSAITWKYPSCILRG
                     DGSQGAFYSIAISNGYQQVDSGTKMIHLGRDTSSRIISKGIAAGHSNNTYRGLVSAHR
                     RATNARNFTNCDSLLIGNKCGAHTVPYIEAKNASATFEHEATTSKISDDQLFYCLSRG
                     LSAEEAVALIVNGFVRDVLQQLPMEFMAETQKLIGISLEGSVG"
BASE COUNT         1771 a         3096 c         3225 g         1797 t
ORIGIN      
        1 tccgccaggg gatcgtccga gcggcgcgac ttgccctcga aatacgcacc ctgttcgatg
       61 gcgaggctct gatggaagat atcgccctcg acatgcgatt gcgcctgaag agtgacgcgc
      121 aagcccctga tcgagccgac cacgcgcccg cggaccacga cgtcctcggc caggacgccg
      181 ccaatcactt gcgacttgtc gccgagcaga agcgagccgc aattgatgtc gccctggatt
      241 tcgccgtcga cctggatctc gcccttcgag gtgacattgc ccgagatgct gaggtcctcg
      301 ccgatgatgg aaggaaccat cttgctcgca gcggagctcg ggctcttgct tgaatacgtt
      361 gcaggccgct cggtgaaggg cgtcttcaac tcgctcgacc gctcgcccat cttcgttacg
      421 ctctctcccg actcgggttt cttggtgaac atgacttgta cctccccatc gcaactgtga
      481 cccgggcgca aagaccactc cccgacgcaa agcttgcgat ccgtcgcgat cccgcgataa
      541 gccgcttcaa gggattgccg cggagtttcc gtcagggccc gaatacgcag cacccactcc
      601 tgccatgaca atatcgtctc gcccgcgcgt tgtcatgcgg gttctcccgg ataaggtagg
      661 ccgcacgccg ggggggctaa gccacgggcg cggggccgga atccgccttc ctttggccga
      721 ttttaccgcc tcttatgcca tcagccctta tccttgcgtc gggcgccaga gcgggtggag
      781 cttaaaggtt tggggcttga cttgggggat accgacacaa ggccgtgagc acgtgaggcg
      841 tcgcgttcgt gtaggggcga ggccctcttt gcgtggctgc ggtcgcctgc ttggtgccgg
      901 gcgagccgtg agtcttcggc gtgcgtcttg ggcttggtcg tggcgggacc tgacggaatg
      961 agcgagccga tcgcaggcgc tctcgcgaga ctgaccaccg agagtggcag agcgatccag
     1021 gtattgcgcg cgacggtgcg cgtggtcacg atccgcgtgc tggcgcaagg gcgccgttgg
     1081 ctgcgcaggc tcgacggcgg cagcggcgcg acgaacccct cgatctcgac cgctctcgtc
     1141 aagctcagca ccgagggcag caaagcgatc ctcgctttgc ccgagcgggg ccgggccctc
     1201 gcaaagcgcg ttcccgagcc ggttcagatc gagctcaaaa aactatcctc gcgcggcgcc
     1261 catgtaactc gcgaaatttt cgccggcatt ctggtggttg gactggttgc catcgttctc
     1321 ggctatggcc ggctggcccg cggcccgatc tccttgagcg gccttgttcc caccatcgaa
     1381 gattcgatca atggcgagct tgccgatctc actgtgaaga tcgacgatgc catcctgcaa
     1441 cgtgcgtccg acggcccggg cgtgctgttc cgtctgcgca atatccggct cgtcgacaag
     1501 aacggcgcga tcctggccca ggcaccgctt gcggccatcg gcatgagcgg aagcgcgctg
     1561 ctatcggggc ggcttgcgcc gggaagcgtc gacttcatcg gcccacggct cctgctcacc
     1621 tacgagcccg gccagggcct ctcgctgagt ttctcgcgtc ttgccccaag cggggccgag
     1681 ccgacgatcc caggcgttcc ggcgccggaa gaggccacgg cgcccgcgga tgaagcacgt
     1741 ccgcccgaat cggtgatcgc caagcggcaa gtccaacgtc agggcagcgg gcgggtgctc
     1801 gacgtgacac gcacggtcgc ggaggtgttc gagaaagcgc gaagcggcga cacttcctat
     1861 ttgacgcggt tcggcgtaaa ggatgcgctc gtcgtgctca atcacggggg cagccaaacg
     1921 tcatggcagg taccggactt cgccatcgat cttgagcatc ataaccagcg aagcattctc
     1981 gtcggccagg ccaacatcgc ctcggccaag ggcgactggc agctcgagat gcgcgccgcg
     2041 gagcagacga agcgccagag cctctccgtc accgcgctga tccaaaacct ggttccgtcg
     2101 ggaatcgccg gaaatttctc ctcgcttggc ctgctcaagg ccctcgacat ggtggtaaac
     2161 ggccagggca cggtcgaact ctccaatgcc ggggacttcg tggccggcga ggccgacttc
     2221 gatttggaga cggggcagat cacgccgcgc tgggatcccg acagtccttt gcggatcgac
     2281 ggcggccgcg tgaggctgag ctatcgcaag gcaaacggcg tgattgaaat cgccccttcg
     2341 acgcttgtgt ggggacaaag ccgggcaacc ttcagcggcg agttccgccc ggcgcgcgac
     2401 gagaggggca cactcaccgc atggaagttc gagctcaggg ccaacgaggc ggtgcttgcg
     2461 gtcgaagagt ccggccttgc gccaatgacc gtcgacgagt ggcaggcgac aggaagcctg
     2521 ttcccgcaag acgggcgcat aagcctgtca cgcttcgtta ttcgcgcggg cgatgcttcg
     2581 atcgcgtttt ccgggacggc ggtcgacacg gaaggtgcgg agggggttca cctcgcgggc
     2641 gagttaagcc cgatgccggt cgatgttttc aagcggttct ggccgaagtt cctcgccggc
     2701 aacgcgcgcg cctgggtgtt ggaccgggtg agcggcggcc aggtcctggg cggcacgttc
     2761 gccgtcaatc tcggtccggc cgagttcgcc gcgatgaaag ccgggcagga gctttctccg
     2821 gaagcggtga aggtcgaact gaaactctcc ggcctgagcg tcgcctatct cccggaaatg
     2881 cccccggtgg tcaccggaga atcgacgctg accgtggccg ggatgacgtt tgcggtcgac
     2941 attcccgaag gccagatcac cccgccctcg ggccggacga tcgcgctcag ccaaggccgg
     3001 ttttccattc cggacctgcg cgccgatccg caacagggcg atatcgtctt caaggccggc
     3061 ggcgagacac cgaccgtgct cgagctgctc gaccacgaac cgctcggcta cctcaagagc
     3121 gtcgggatga agccggactt tctcgcgggc acgaccgagg gcgatttttt cctgtcgatg
     3181 cccctcttgg ccgatctcga attcgagcag atcaaggtca acggctcggc gcgcctgaat
     3241 gacgcaattg ccgggaccct cgccgacaac gtcgacgtgg cgggcggttc gctcgatgtg
     3301 actctgagcg agcaagggct cgaggccatg ggtgcgatca gcatcaaggg cgtcccggcc
     3361 gagctctcct ggcagcgcat cttcttcgcg cccgacgccg agcagccgcc aattcaggtc
     3421 acggcgcgcc tcgatgccgc gacgcgcgag cagctcggac tgaaggtcaa ccatctggtc
     3481 cagggcccca cgcctgtcac gctgcgcgtg gcggggctcg gcacggaaac gccaacgggc
     3541 atgactctcg aagccgatct caccgaggcg cagcttctgt ttggcggctt gggctggacc
     3601 aagccatccg gccgcgccgc caccctccac ctcgacgttc tccagaatga ggatggttcg
     3661 accgtcctcg acaatctcaa gatcgtcggc gacgacatcg acatccgcgg cggcatcgtg
     3721 ctcgacgcag agcagcacct caaggagttt cacttctccg acttctcggt caaccagctc
     3781 agccatgtcg agatcacggc ggcggtccgc gacgaccagg tgctcgaaat caaggccgag
     3841 ggcccgagct atgatggaag acagttcttc gaatccctgt tttcggccgg acaactcgaa
     3901 ggttcggcgg agcccgacga gcctttcggc gtcgatttga cagccagcat cggcacggtt
     3961 gtcggcttct acgatacgac ggcgcagaat gtcgaagtga ccatgaagaa gcgcaacggc
     4021 cggatcgttg cgctcgatgc caaggccggg cttaacggca aggcgccggc cgcagtgcag
     4081 ctcgtgcaaa agcagggggc acgcatgatc gaggccgaag cgcgcgacgc cggtgccgcg
     4141 ttccggctcg tcggattcta cccgagcgtc gaaggcggag aggcatcgct tcaagtcaat
     4201 ctcgacgccg ggggagccgg gaccaagagt ggaacgctgt gggcgcgcaa tttcgtcgtg
     4261 ctgggtgatt ccgtcgtagg cgaagttctc accgatccga actcggccgc ggtgttggga
     4321 ccctccgagc ggaagcagca ggtgacccgg cagcgcatcg ccttcagtca gttgcgcgcg
     4381 cccttttccg ttggcggcgg aaaattccgg ctgcatgacg cttacatcaa cggaccgctg
     4441 cttggcgcga ccatgcgcgg cacggtcgat ttcaaggcgg agatggtcga gctcgggggc
     4501 acctacgtgc cgctttacgg tttgaactcg gcgctcgggt cgattccact cctcggccgc
     4561 gtgctggtcg ggcgcgaggg tgagggcgtg gtcggcatca ccttcgccat caaaggcaag
     4621 ctcgacgatc cgtcggtgct ggtcaacccc atgtcggtga tgacgccggg catcttccgg
     4681 cagatcttcg agttcaccgg ctcggtgccg gaccccaatg ccaagcctga ggcgtcgtcc
     4741 tacatggtgc ccggccagca attcgggcag cccgccccgc aatagtctag accggcctga
     4801 tgagcgcgtg gcgcttcttg ccgagcgaga gcttgatgac gccctcgggc gagagatcgg
     4861 cgagcgtcaa tttgcgcttt tcgtcggtca cggcgacgtc gttgaccttg atgccgccgc
     4921 tcctgatctg acgtcttgcc tcgccggtcg aggcgacgaa tccgatttcg acgttggcgc
     4981 taagaacgcc ccatcccttt tccagctctg agcggggaat gtcaaaggtg gggagcgtca
     5041 cggcgatctc gccctgctcg aaggtcgccc gcgccgtctc ggccgcacga tcggctgcgt
     5101 cccgcccatg cacgagcgcg gtagcctcgg tcgcgaggat cttcttcgcc tcgttcagct
     5161 ccgcgccgcg aagtttggcg agccttgcga tctcgtcgag gggaagctcg gtgaacagcc
     5221 tgaggaagcg ctcgacgtcg ccatcctcgc tgttccgcca atactgccaa tagtcgtacg
     5281 gggacaccat gtcgggattg agccacacgg cgccggcggc ggtcttgccc atcttggcgc
     5341 ccgacgcggt ggtgagaagg ggcgaggtga gtccgaacag ctccgccccg tcggtgcggc
     5401 gtccgagctc gatgccgctc acgatgttgc cccactggtc cgatccaccg agttgcaggc
     5461 ggcagccatg gcgccggtag agctcgacga agtcgtaggc ctgcagcacc atgtagttga
     5521 actcgatgaa gctgagggcc tgctcgcgct ccagcctgag cttgaccgag tcgtaggtca
     5581 gcatgcggtt gacgctgaaa tggcgcccgt agtcgcgcag gaaatcgatg tagttaaggc
     5641 catcgagcca atcggcgttg ttgatcatga gcgcgtcgac ggcgccgcta ccgaagctca
     5701 ggaacttggt gaacacttgc tccatcccag cagtgtttgc ggcgatctcg gtctcgctca
     5761 ggagcttgcg ggcctcgtcc ttgccggaag ggtcgccgac cttggttgtg ccgccgccga
     5821 tgaggacgat cggccggtgc ccggtctgct gcagcttgcg cagcatcatg atgctgacca
     5881 ggtggccaac atggagcgag ggcgcggtgc agtcgaagcc gatatagccg gtcagccgtt
     5941 cgcttcgcgc aagccgatcg agccccgcct cgtcggtcag ctggtggatg aacccccgcg
     6001 ccgcgagctc ttgcagaaag tccgatttga gcttgctcat gggcgcggct tggaaggatg
     6061 gcaaccgcaa agcctgtatc agctttgccg caagaatgca cgggagcgaa agcggcttcc
     6121 ccggggcgac ttaagcggcg gagtctttcc gccttttcgt cttgccgagc cgcttgtcga
     6181 ggtaatgatc cacgacctcc atcagcccgt ccatccgccc ctcgaagaaa tggttcgcat
     6241 ccggaaccgt cgcgtggtcg atcacgatcc ccttttgcgt cttgagacgc tcgacgagcc
     6301 cgcgcacgga ttccgttggc acgactcgat cttttgcccc gttcaccatc aggcccgagg
     6361 aggggcaggg ggcgaggaag gtgaagtcgt agagattggc ggggggtgct accgaaatga
     6421 agccctcgat ctcggggcgc cgcatcagga gttgcatgcc gatccaggcg ccgaacgaca
     6481 cgccggcaat ccagcaggtc gcggcgtcgg cattgtaggc ctgtagccaa tcgagcgcgg
     6541 tggcggcgtc ggcaagttcg ccggggccgt tgtcgaacag gccctcgctt cgcccgaccc
     6601 cgcgaaagtt gaaccgcagc acggagaatc cgcggcgctg gaaggagtaa tacaggtggt
     6661 agacaacctg gttattcatc gtcccgccaa attgcgggtg ggggtgcagg atgagcgcga
     6721 cgggagcgtt aggccctttt tcgtggtggt agcgggcctc gatccttccg gcgggaccgc
     6781 tgatattcac ttcaggcatg gatgagatgt ctcgacacgg tgtggttcat gcgtcatgag
     6841 ggtccgagcg gaaggatcgg gtggggatgg atttggggag tgcggacaga agctgcaaaa
     6901 cttgactctg cggctccgga aacctatacc ctgtataaga ttagaattgt tccaaacaag
     6961 ccgctccccg atagctgggc gcttggagcg gtgaaacttc aggccgcgct tcatagcacg
     7021 tgcgatgggg gcacgtgcaa gcgttaagcg tctgcgattg gccgcgagct tcgtgagagc
     7081 atgaaccagc gctcctatct cgattacaac gcgactgcgc cgcttcggga cgaagtgcgc
     7141 gaggctgtcg tcgaggcgct ttcgatccat ggcaatccgt cgtcggttca tgccgaggga
     7201 cgcgacgcgc gccgggcggt cgaggccgcg cgggagaaga ttgcggcgct cgcgggcggt
     7261 aaggcggaag acgtggtctt tacgagcgga gccactgagg caaatgcgct cgcgcttgca
     7321 ccgcgtgccg gggcgagatg gcactgttac gtctcagcgg tggagcatcc ttccgtgctc
     7381 tccggcggac ggttcgcggc ggggtccgtc tctcgacttc ccgtcacggc tgacgggtgc
     7441 gccgacctcg gaatccttgc ggagaagctt gccgagtccg atcgcgcggg tgtgaggccc
     7501 ttcgtgtcgc tgatggccgc caacaatgag accggcgcga tccagccggt cgcagaggcc
     7561 gcgcgcgtcg tgcatgacgc cggcggggtc ctccacaccg atgccgttca ggcggcggga
     7621 cggatcaagc tcgatatgga cgcgctcggc gccgacatgc tgagcctctc cgcccacaag
     7681 atcggcggtc ccaagggtgt gggcgcgctc gtgctccacg acggtgcatc ggtcgagccc
     7741 ctgatcaagg gcggcagcca ggagcggcgg cgccgcgcgg gaacggagaa cgtggcgggc
     7801 attgtcggat ttggcgtggc ggccgagctt gccttggccg acctcgccaa ggccgaaaaa
     7861 accgcaaggt tgagagatga gcttgagcag ggcgcgcttt cgctcgcgcc cgacgccgtc
     7921 gtgctttcgg ggcgggtcga tcgtctgccg aacacgagct gccttgccgt gccgggaatc
     7981 aaggccgaga cgctcgttat ccggctcgat ctcgcaggaa tcgccgtgag cgcgggcagc
     8041 gcgtgctcgt ccggcaaggt cgaggcgtcc catgtgctcg ccgcaatggg cctttccgac
     8101 gagcttgcgc gcggaggcat ccgcgtaagc cttggttttg ggacctcgcg taacgatatc
     8161 aataccttcc tcaaggcctt tggcgaactt attgggcggc tcaggcctgg ggtggcggcc
     8221 taagggccgc cgagacgatt aggagaagag atatgccagc cgttcaggaa accgtcgctc
     8281 gcgtcaaaga gatcgacgtc gacaagtaca aatacggctt caccaccgat atcgagtcgg
     8341 tcaaggcgcc taagggcctg aacgaagacg tggtccgctt catctcggcc aagaagggcg
     8401 agccggactg gctgcttgcc tggcggctcg aggcctatcg ccgctggctc accctggaag
     8461 agccgacttg ggccaatgtc cactacccca agatcgattt ccaggagctc tactactact
     8521 cggcgcccaa gagcacggag gggccaaaga gtctcgacga ggttgatccc gagctgctca
     8581 ggacctatgc gcgcctcggc attccgctct cggagcagaa ggtgctcgcg ggcgtcgagg
     8641 gcgcaagcct cgaagggcgg gtcgcggtcg atgccgtgtt cgattccgtc tccgtcgtga
     8701 ccaccttcaa ggaagagctg gcaaaggccg gcgtgatctt ctcctcggtc tccgaggcgg
     8761 tgaagacgca tcccgagctg gtgcggaagt acctgggctc ggtggtgccg atcaccgaca
     8821 atttctatgc cacgctcaac tcggccgtgt tttccgacgg cagcttcgtc tatgtgccgc
     8881 agggcgtccg ctgcccgatg gagctctcca cctatttccg catcaacgag aagcagaccg
     8941 gacagttcga gaggacgctc atcatcgccg acaagggctc ctacgtaagc tatctcgaag
     9001 gctgcacggc gccgatgcgc gacgagaacc agcttcacgc cgcggtggtc gagcttgtcg
     9061 cgctcgacga cgccgagatc aaatattcca cggtgcagaa ctggtacccc ggcgacaagc
     9121 agggcaaggg cggcatctac aatttcgtca ccaagcgcgg cgattgccgc gggcatcatt
     9181 ccaaaatctc gtggacccag gtcgagaccg gttcggccat cacttggaaa tatccgagct
     9241 gcatcctgcg cggcgacggc tcgcagggcg cgttctattc gatcgccatc tccaacggct
     9301 atcaacaggt cgattccggc accaagatga tccatctcgg gcgcgacacg tcgagccgca
     9361 tcatctccaa gggcatcgcc gccggccatt ccaacaacac ctatcgcggg ctggtctcgg
     9421 cgcatcgccg cgccacgaat gcgcgcaact tcaccaattg cgactcgctt ctcatcggca
     9481 acaaatgcgg cgcccacacc gtgccctata tcgaggccaa gaacgcgagc gccaccttcg
     9541 agcacgaggc gaccacctcg aagatttccg acgatcagct gttctattgc ttgagccgcg
     9601 ggctgtcggc ggaggaggcc gtcgcgctga tcgtcaacgg cttcgtccgc gacgtgctcc
     9661 agcagctccc gatggagttc atggccgaga cgcagaagct gatcggcatc agcctggaag
     9721 ggtcggttgg ctagagtctc atcagcgcag tcaactaggc atgactcagt tatgaagaca
     9781 atcgcaacga tcaatttcaa gggcggcgtg gagacgcaat aacaaatcgt cttcaagggc
     9841 ggcgtggaga cgcaataaca aatcgtcgaa cccctgatga actcgtgcc
//
LOCUS       DASUQP010000174         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1178244, whole genome shotgun sequence.
ACCESSION   DASUQP010000174 DASUQP010000000
VERSION     DASUQP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178244"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1610
                     /locus_tag="VFY74_07630"
     CDS             225..1610
                     /locus_tag="VFY74_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5931603.1"
                     /translation="MPELDPLLLSRIQFAFTISFHILFPSFTIGLAAWLVVLEALWLK
                     TGRAIYRETALHWTKIFAVSFGMGVVSGVVLSYAFGTNWSELSRRGGNVIGPLMSYEV
                     LTAFFLEAGFLGIMLFGWNRVHKWVHFFASCMVALGTVISAFWILAANSWMQTPVGFR
                     VADDGVLHVTDWWQVIFNPSFPYRFAHMVAAAYLTTAFIVAGIGAWYILRERSVPHGR
                     IMLGMGLSMLVWLAPLQLVIGDLHGLNAREHQPAKIAAVEAHWETQRRAPLILFALPD
                     PAAEKNNYEIAIPALGSLVLTHEWDGEIRGLKSFPPEDRPNPIIPFFAFRIMVGIGLI
                     MIAVGLVGSVLWLRGRLYSSRWFLRTLVWVSPLGFVAVLAGWFVAEVGRQPWTVYGIL
                     RTADSVSPIPGASVLASLILFVVVYTIIFGAGVYFIAKLVQRGPDETPPVPEADEADL
                     SHRPMAAAGNP"
     gene            1607..2641
                     /gene="cydB"
                     /locus_tag="VFY74_07635"
     CDS             1607..2641
                     /gene="cydB"
                     /locus_tag="VFY74_07635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5931604.1"
                     /translation="MTGTASQMTEFLPVILPLVWAAILALAVFLYVLLGGYDLGLGAL
                     FPLAPSDKDRDAMMASVAPFWDGNETWLVLGGGGLLAAFPLAYSIMLPALYIPIILML
                     MGLILRGVAFEFRFKSERSRWLWDLAFSAGSMLAALMQGVIMGAFIEGFAVTDNKFSG
                     GAFDWLTPFTAVTAAALLSGYVLLSTGWLIMKADEALREWAYRIAYTALIVVAAFIVI
                     FSIWTPFLHPEIAARWFQPANMVMLSPVPLATAAAVGALWLALNRRQRYTPFLLGVAI
                     FILCYTGLAVSLFPYIVPPDITIWQAAAAPDSQLFMLYGAIPLLPIILGYTVYSYYVL
                     WDAPEHDAYH"
     gene            complement(2833..>3513)
                     /locus_tag="VFY74_07640"
     CDS             complement(2833..>3513)
                     /locus_tag="VFY74_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5931605.1"
                     /translation="FNLNFGPVVDLSLNPSNYVIVQRNRSFGADPRAVATLAAAFIRA
                     HREAGVATVAKHFPGHGSSSADSHKMFTDISESWQEIEIEPYRTLAKDGLLDAVMLGH
                     LYHPRFSDGAKLPASLSGRAVSALRDKGSIGFKGVVVSDDMEMGAVRDDYALEERVVK
                     AVNAGTDLLVFSNVTLRDPELGVKLHAIIADAVRNGRISRSRIEKAYGKISLLKRRIA
                     THDLPGKW"
BASE COUNT          525 a         1160 c         1087 g          741 t
ORIGIN      
        1 gcgcccgcgc gctcgccgcg ttcaagcgcg cgcagaagcg ctagagcacg cgcctcacct
       61 gcgaacggcg ttgacatacg agatgtaaag ccgagcgcgc ccgtccattg ctcttatggg
      121 tttccgcgcc gcatcgggag cgttagtctg ctgcggaacg cgattcgtcc ccgccgccga
      181 cgtggtggga gccgcggccg accttgggaa ggcaacgctt cgacatgcca gagctcgacc
      241 cgctgttgct gtcgcgcatc cagttcgcct tcaccatctc gttccacatt ctgtttccgt
      301 ccttcaccat cgggctcgcg gcctggctcg tcgtgctcga ggcgctttgg ctcaagaccg
      361 gccgcgcgat ctatcgcgag accgcgctgc attggaccaa gatcttcgcg gtctccttcg
      421 gcatgggcgt ggtctcgggc gtcgtgcttt cctatgcctt cggcaccaat tggagcgagc
      481 tgtcgcgccg gggcggcaat gtcatcggcc cgctgatgtc gtacgaagtg ctgaccgctt
      541 tctttctcga agcgggcttt ctcggcatca tgctgtttgg ctggaaccgc gtgcataaat
      601 gggtgcactt cttcgcaagc tgcatggtgg cgctcgggac cgtgatctcg gccttctgga
      661 ttctcgcggc caatagctgg atgcagaccc cggtcgggtt ccgcgtcgcg gacgatggcg
      721 tgctgcacgt gaccgactgg tggcaggtga tcttcaaccc gtcctttccc taccgcttcg
      781 cccatatggt cgccgcggcc tatctcacca ccgccttcat cgtcgcaggc atcggcgcct
      841 ggtacatcct gcgcgagcgc agcgtcccgc atggccgcat catgctcggc atggggctga
      901 gcatgctcgt ctggcttgcg ccgctccagc tcgtgatcgg cgacttgcat ggactgaacg
      961 cacgcgagca ccagccggcc aagatcgcgg cggtggaggc ccattgggaa acgcagcggc
     1021 gcgccccgct catcctgttc gcgcttcccg atcctgccgc cgagaagaac aattacgaga
     1081 ttgcgattcc ggctctcggc agcctcgtcc tgacccacga atgggatggc gagatcagag
     1141 ggttgaaatc cttcccgccc gaggaccggc ccaatccgat catcccgttt ttcgccttcc
     1201 gcatcatggt gggcattggg ctgatcatga tcgccgtggg gctggtcggc tcggtcctgt
     1261 ggctcagggg acggctttat tcgagccgct ggttcctccg gactctcgtg tgggtctcgc
     1321 cgcttggctt cgtcgccgtg ctcgcgggct ggttcgtggc ggaggtggga cgccaaccct
     1381 ggaccgtcta cggaatcctg cgaacggccg actccgtctc tcccattccc ggggcgagcg
     1441 tgctcgcttc gctcatcctg ttcgtcgtgg tctacaccat catcttcggc gccggcgtct
     1501 acttcatcgc caagctggtg cagcgcggtc cggatgaaac gccgccggta cctgaggcgg
     1561 acgaggcgga tctttcccat cggccgatgg ccgcggcagg aaacccatga ccgggacggc
     1621 aagccagatg acggagttcc ttccggtgat tttgccgctc gtctgggcgg cgatcctggc
     1681 gctcgcggtc ttcctttacg tgctgctcgg cggctacgac ctaggccttg gcgcgctctt
     1741 cccgctcgcg ccctccgaca aggaccgcga cgcgatgatg gcatcggtgg cgccgttctg
     1801 ggacggcaac gagacgtggc tcgtcctggg cggaggcggt ctgctcgcgg cctttccgct
     1861 cgcctattcg atcatgctgc ccgcgctcta tatccccatc atcctgatgc tgatggggct
     1921 gatcttgcgc ggcgtcgcct tcgagttccg cttcaagtcc gagcgttccc gctggctatg
     1981 ggacctggcc ttcagtgcgg gctcgatgct cgccgctctg atgcagggcg tcatcatggg
     2041 cgccttcatc gaaggctttg ccgtaaccga caacaaattc tccggcggcg ccttcgactg
     2101 gttgacgccg ttcacggcgg tgaccgcggc ggcgctcctc tccggctatg tgttgctgtc
     2161 aacgggatgg ctgatcatga aggccgacga agccttgcgc gagtgggctt accgcatcgc
     2221 ctataccgcg ctgatcgtgg ttgccgcatt tatcgtcatt ttcagcatct ggacgccgtt
     2281 cctccatccc gagatcgccg cgcgatggtt ccagccggcc aacatggtca tgttgagccc
     2341 ggtgccgctc gctaccgcgg cggccgtcgg cgccttatgg ctcgccctga accggcgcca
     2401 gcgatacacg cccttcttgc tcggcgttgc gatcttcatc ctgtgctaca cggggctcgc
     2461 ggtcagcctc ttcccctata tcgttccgcc cgacattacg atctggcagg cggcggccgc
     2521 gcccgacagc cagctcttta tgctatacgg cgcgatcccg ctcctgccga tcatcctcgg
     2581 ttataccgtg tactcttact acgtgctatg ggacgcgccc gagcacgacg cctatcattg
     2641 accgtcttat tgtcatcgcc cggctgcctg ccttcgccga agcgaagccg tcccttggtg
     2701 gcgttttgat gcagtaggcg cttcggcttc gcgcaggcag gatcgggcga tccagtaacc
     2761 cagttgctgc tgatcaactc cccaggggct agtggattcc cgcgttcgcg ggaatgaccg
     2821 ttacgggaga catcaccact tgccggggag gtcgtgcgta gcgatgcggc gcttcaagag
     2881 gctgatcttg ccataggcct tctcgatgcg ggaccgggag atgcgcccgt tccgcaccgc
     2941 atcggcgatg atggcatgaa gcttcacgcc gagctcggga tcgcgcagcg ttacgttcga
     3001 aaacacgaga agatcggttc cggcattgac ggccttcacc acccgctctt cgagcgcata
     3061 gtcgtccctg accgcgccca tctccatgtc gtcgctcacc acgacgcctt tgaacccgat
     3121 ggagcccttg tcgcgcagcg cactcacggc ccgccccgat aacgaggccg gcagcttcgc
     3181 gccgtcgctg aagcgagggt gatagaggtg cccgagcatc acagcatcga gcagcccgtc
     3241 cttggcgagc gtccggtaag gctcgatctc gatctcctgc caactctcgg agatgtcggt
     3301 gaacatctta tggctgtcgg cgctgctcga gccatgccca gggaagtgct tggccacggt
     3361 cgcgacaccg gcctcgcgat gggcgcggat gaaggcagcg gccagcgtcg cgacggcacg
     3421 aggatcggcg ccgaaactcc ggttccgctg gacgatgacg taattgctcg ggttgaggct
     3481 gagatcgacg acgggaccga aattgagatt gaa
//
LOCUS       DASUQP010000175        21273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1178763, whole genome shotgun sequence.
ACCESSION   DASUQP010000175 DASUQP010000000
VERSION     DASUQP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 21273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21273
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178763"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="fusA"
                     /locus_tag="VFY74_07645"
     CDS             <1..870
                     /gene="fusA"
                     /locus_tag="VFY74_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171081.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5931606.1"
                     /translation="FPEPVIEVAVEPKTKGDQEKLGVALNRLAQEDPSFRVAVDHESG
                     QTIIKGMGELHLDIIVDRMRREFKVEANVGAPQVAFRETVSKRADVDYTHKKQTGGSG
                     QFARVKMTIEPNEQGKGYEFENKVVGGNVPKEYIPGVQKGVQSVVESGVLAGFPILDV
                     KVTLTDGAYHDVDSSVMAFEIAGRAAFREGALKAGPKLLEPIMKVEVVTPEDYVGGII
                     GDLTSRRGQVRGQETRGNAAVINAMVPLANMFGYVNNLRSMSQGRAQFTMQFDHYAQA
                     PQGVAEEVKAKFA"
     gene            936..2126
                     /gene="tuf"
                     /locus_tag="VFY74_07650"
     CDS             936..2126
                     /gene="tuf"
                     /locus_tag="VFY74_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX5931607.1"
                     /translation="MAKEKFQRTKPHCNIGTIGHVDHGKTTLTAAITKVLAEKGGAKF
                     TAYDEIDKAPEEKARGITISTAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPALVVYLNKVDMVDDPELLDLVELEVRELLSK
                     YEFPGDEIPIVKGSALAALEGKDEQVGKTSVVELMKAVDDYIPQPDRPKDKPFLMPIE
                     DVFSISGRGTVVTGRIERGIIKVGEEVEIVGIKPTTKTTVTGVEMFRKLLDQGEAGDN
                     VGCLLRGIDREAVERGQVLAKPGSVTPHTKFKAEAYILTKEEGGRHTPFFGNYRPQFY
                     FRTTDVTGVVHLPEGTEMVMPGDNIAMTVELIVPIAMEEKLRFAIREGGRTVGAGVVA
                     SIIE"
     gene            2186..2494
                     /gene="rpsJ"
                     /locus_tag="VFY74_07655"
     CDS             2186..2494
                     /gene="rpsJ"
                     /locus_tag="VFY74_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005773267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX5931608.1"
                     /translation="MQSQNIRIRLKAFDHRILDASTKEIVNTAKRTGAEVRGPIPLPT
                     RIEKFTVNRSPHIDKKSREQFEMRTHKRLLDIVDPTPQTVDALMKLDLAAGVDVEIKL
                     "
     gene            2526..3308
                     /gene="rplC"
                     /locus_tag="VFY74_07660"
     CDS             2526..3308
                     /gene="rplC"
                     /locus_tag="VFY74_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5931609.1"
                     /translation="MRSGVIAQKVGMTRIFTDAGEHIPVTVLRLEHCQVVGHRTSEKN
                     GYTALQLGAGRPKVKRLSRAARGHFAVANVEPKRKLAEFRVSPDNLIEVGAEITADHF
                     VEGQYVDVSGTSIGKGFAGAMKRHGFAGLRASHGVSVSHRSHGSTGQRQDPGKVFKGK
                     KMAGHMGDVNVTTQNLRVVKTDPERGLIMIRGAVPGAKGGWVLLRDAVKRALPEAAPK
                     PGAFRKSYQPEQAKKAKARKTKSAESAESAASEAGEGGSQES"
     gene            3305..3925
                     /gene="rplD"
                     /locus_tag="VFY74_07665"
     CDS             3305..3925
                     /gene="rplD"
                     /locus_tag="VFY74_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5931610.1"
                     /translation="MKADVTTLDAKKAGTVELPDDLFGLEPRPDILQRMVRYQLAKRR
                     SGTAHVKDRSEINATTAKLYRQKGTGRARHGSKKAPIFRGGGKAFGPKPRSFAVGLPK
                     KVRALALKHALSAKAKSDTLIVIDKCELKDGKTKELKSRFAKLGFGSVLIIDGASVQE
                     AFAKAARNLPEVDVLPVQGINVYDILRHEKLVLTKAAIEALEARFK"
     gene            3922..4215
                     /locus_tag="VFY74_07670"
     CDS             3922..4215
                     /locus_tag="VFY74_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005773270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5931611.1"
                     /translation="MTTRTDYDIILAPVITEKATAVSEANQVVFKVRRDATKPEIKAA
                     VETLFKVKVLSVNTLTRKGKAKNFRGVRGRQQDVKKAIVRLAEGQTIDVTTRI"
     gene            4220..5053
                     /gene="rplB"
                     /locus_tag="VFY74_07675"
     CDS             4220..5053
                     /gene="rplB"
                     /locus_tag="VFY74_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5931612.1"
                     /translation="MALKTYKPTTPSQRQLVLIDRSQLWKGAPVKALTKGLSKTGGRN
                     NHGRVSMWHRGGGHKRAYRKVDFKRGKLGVPATVERLEYDPNRTAFIALIKYEDGELS
                     YILAPQRLAVGDTVISDLRADVKPGNAMPLANMPIGTIIHNVEMKKGKGGQIARAAGT
                     YVQLVGRDAGYAILKLNSGETRMVPSECMASVGAVSNPDNSNISIGKAGRARWKGRKP
                     VVRGVAMNPIDHPHGGGEGRTSGGRHPVSPWGKSAKGKKTRSNKSSNKFILRSRHQKK
                     G"
     gene            5059..5349
                     /gene="rpsS"
                     /locus_tag="VFY74_07680"
     CDS             5059..5349
                     /gene="rpsS"
                     /locus_tag="VFY74_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004855734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX5931613.1"
                     /translation="MGRSIWKGPFVDGYLLKKAEKARGSGSNAVIKMWSRRSTILPQF
                     VGLTFGVHNGHKHIPVLVTEDMIGHKFGEFAPTRTFHGHAADRKAKRVTKEA"
     gene            5349..5729
                     /gene="rplV"
                     /locus_tag="VFY74_07685"
     CDS             5349..5729
                     /gene="rplV"
                     /locus_tag="VFY74_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX5931614.1"
                     /translation="MGKPQRERALKENEARAVTRLVRVSPQKLNLLAQLIRGKKVDRA
                     LADLTFSRKRVAKDVKKTLQSAIANAENNHDLDVDALVVAEAYVGKNLVMKRMRARAR
                     GRAGRIEKPFAQITVVLRQVEEPA"
     gene            5729..6448
                     /gene="rpsC"
                     /locus_tag="VFY74_07690"
     CDS             5729..6448
                     /gene="rpsC"
                     /locus_tag="VFY74_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX5931615.1"
                     /translation="MGQKVNPIGLRLGVNRTWDSRWYASRGEYARLLHEDLKMREHIL
                     ESRKQAGISKVVIERPHKKCRVTVHTARPGILIGKKGADIEKLRRELANMTDSEVHLN
                     IVEVRKPEIDATLVAEGIAQQLERRVAFRRAMKRAVQSAMRMGALGIRINCGGRLGGA
                     EIARMEWYREGRVPLHTLRANIDYGTALGRTAYGIIGVKVWIFKGEIMEHDPMAQENK
                     ALEAQEGGRSTGRREPAQAQG"
     gene            6464..6883
                     /gene="rplP"
                     /locus_tag="VFY74_07695"
     CDS             6464..6883
                     /gene="rplP"
                     /locus_tag="VFY74_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX5931616.1"
                     /translation="MLQPKRTKYRKAHKGRIKGAASSAATLNFGTYGLKAQEPARITA
                     RQIEAARRAMTRQMKRSGRVWIRLYPDVPVSKKPTEVRMGKGKGTPEFWVARVKPGRI
                     MFEIDGVTEQVARESLRLAAAKLPIKTRMVARIEVKP"
     gene            6880..7080
                     /gene="rpmC"
                     /locus_tag="VFY74_07700"
                     /pseudo
     CDS             6880..7080
                     /gene="rpmC"
                     /locus_tag="VFY74_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
     gene            7231..7476
                     /gene="rpsQ"
                     /locus_tag="VFY74_07705"
     CDS             7231..7476
                     /gene="rpsQ"
                     /locus_tag="VFY74_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX5931617.1"
                     /translation="MPKRVLQGVVVSDKNDKTVVVRVERRFTHPVLQKTVRRSKNYQA
                     HDENNEFKVGEMVWIEECPPVSKQKRWTVLPREQAAR"
     gene            7507..7875
                     /gene="rplN"
                     /locus_tag="VFY74_07710"
     CDS             7507..7875
                     /gene="rplN"
                     /locus_tag="VFY74_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX5931618.1"
                     /translation="MIQMQTNLDVADNSGARRVQCIKVLGGSKRKYASIGDTIVVSVK
                     DAIPRGRVKKGQVMKAVVVRTAKGVRRPDGSIIRFDRNAAVLINPQGEPIGTRIFGPV
                     TRELRAKKHMKIVSLAPEVL"
     gene            7875..8195
                     /gene="rplX"
                     /locus_tag="VFY74_07715"
     CDS             7875..8195
                     /gene="rplX"
                     /locus_tag="VFY74_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX5931619.1"
                     /translation="MAKLKIKKGDHVVVITGKDKGKKGEVLKVMPEENRAIVKGVAMV
                     RRHQRQSPSQEGGIIAKEAPIHISNLALEDPKDGNPTRVGYKFLKDGRKVRFARRSGE
                     VIDG"
     gene            8188..8745
                     /gene="rplE"
                     /locus_tag="VFY74_07720"
     CDS             8188..8745
                     /gene="rplE"
                     /locus_tag="VFY74_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX5931620.1"
                     /translation="MAETTYIPRLKQHYNEVLKPALAKEFGYTNAMQVPRLEKVVLNM
                     GVGEGVSDSKKVQAAAADLGMIAGQKPVITRARKSIATYKLREGMSIGAKVTLRQNRM
                     YEFVDRLVNIALPRVRDFRGLNPKSFDGRGNFALGLKEHIIFPEIDYDKVEDVLGMDI
                     IVCTTARTDDEARALLKGLNFPFRS"
     gene            8779..9084
                     /gene="rpsN"
                     /locus_tag="VFY74_07725"
     CDS             8779..9084
                     /gene="rpsN"
                     /locus_tag="VFY74_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX5931621.1"
                     /translation="MAKTSTIERDKKRRRLAKKYKLRRTRLKAIAADRSLPAEERFAA
                     RLKLAELPRNSSPTRIRNRCDLTGRPRGYYRKLRMSRIALRDLGSQGLVPGMIKSSW"
     gene            9094..9492
                     /gene="rpsH"
                     /locus_tag="VFY74_07730"
     CDS             9094..9492
                     /gene="rpsH"
                     /locus_tag="VFY74_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX5931622.1"
                     /translation="MSINDPLGDMLARIRNAQMRHKTTVTTPASKLRERVLDVLAEEG
                     YIRGYARVDYSGGRSEFEIELKYFDGEPVIKDIKRVSTPGRRVYSSVKDLPTVANGLG
                     VAILSTPKGVMSDARARTENVGGEILCNVF"
     gene            9521..10054
                     /gene="rplF"
                     /locus_tag="VFY74_07735"
     CDS             9521..10054
                     /gene="rplF"
                     /locus_tag="VFY74_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX5931623.1"
                     /translation="MSRIGKKPVSVPGGVTAAVNGQEVKIKGPKGELKHVLADAMVAK
                     LDKDGIEITMREDTKEARAMWGMSRTLIANLIAGVTEGFSKRLEISGVGYRAAVQGSN
                     LQLQLGYSHDVAYPIPQGIQIACPKPTEIVITGIDKQKVGQVAAEIRRFRPPEPYKGK
                     GIKYAGEFILRKEGKKK"
     gene            10069..10431
                     /gene="rplR"
                     /locus_tag="VFY74_07740"
     CDS             10069..10431
                     /gene="rplR"
                     /locus_tag="VFY74_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5931624.1"
                     /translation="MGALNESFMRRSRRVRHTLKRAANGRARLSVFRSSRHIYAQVID
                     DEAGKTLAAASTLDKDLKGALPKGSDLAAAQAVGKLLAERALKAGVKDVVFDRGGYIY
                     HGRVKALADAAREAGLNF"
     gene            10456..11028
                     /gene="rpsE"
                     /locus_tag="VFY74_07745"
     CDS             10456..11028
                     /gene="rpsE"
                     /locus_tag="VFY74_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX5931625.1"
                     /translation="MARGQQARDREERDRDSEFIDKLVHINRVAKVVKGGRRFGFAAL
                     VVVGDQKGRVGFGHGKAREVPEAIRKATEAAKRNLVKVPLREGRTLHHDVTGQHGAGR
                     VVLRAAPPGTGIIAGGPMRAVFETLGIQDVVAKSLGSSNPYNMVRATFDALRHEDSPR
                     GVAARRGKKVSEIVSRRRDGAGDAAGAPEA"
     gene            11065..11268
                     /gene="rpmD"
                     /locus_tag="VFY74_07750"
     CDS             11065..11268
                     /gene="rpmD"
                     /locus_tag="VFY74_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX5931626.1"
                     /translation="MAEKRKTIVVEQIGSPIRRPASQRATLIGLGLNKMHKRRTLVDS
                     PQVRGMVAKVSHLVRIVDDEPKA"
     gene            11281..11727
                     /gene="rplO"
                     /locus_tag="VFY74_07755"
                     /pseudo
     CDS             11281..11727
                     /gene="rplO"
                     /locus_tag="VFY74_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            12060..13394
                     /gene="secY"
                     /locus_tag="VFY74_07760"
     CDS             12060..13394
                     /gene="secY"
                     /locus_tag="VFY74_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX5931627.1"
                     /translation="MVSAAEQLVSNLNFAAFAKADELKKRIWFTLGALLIYRLGTYIP
                     IPGINPEALADVFRQQQSGILGMFNMLSGGAIGRMAIFALNIMPYISASIIIQLLTTV
                     SPHLEQLKKEGEQGRRQINQYTRYGTVLLAALQGYGIAIGLEGAGNVVVDPGWFFRIT
                     TVITLVGGTVFLMWLGEQITARGVGNGISLIIFAGIVAELPSALIGTLELGRQGALST
                     ALIIGLLGMAVLVIAFIVFMERAQRRLLVQYPKRQIGNRMFQGDSSHLPLKLNSAGVI
                     PPIFASSLLLLPITLANFSAGQGPDWLNSITAMLGMGQPLHIVLYVAMIIFFAFFYTA
                     VVFNPKDTAENLKKYGGFLPGIRPGERTAEYIDYVLTRITVVGALYLAAVCVLPELLI
                     SYTGVPFYFGGTSLLIVVSVTMDTVAQIQSHLLAHQYEGLIKRARLKGARRR"
     gene            13391..14041
                     /locus_tag="VFY74_07765"
     CDS             13391..14041
                     /locus_tag="VFY74_07765"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007531684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5931628.1"
                     /translation="MNLVLLGPPGAGKGTQAKLLEESRSLVKLSTGDMLRAAVAAGTE
                     LGRKAGDIMERGQLVPDVLVINLIAERLDQDGGGQGFILDGFPRNIAQAEALDRLFKE
                     RGKAVDRVIVMDVDDEALVERIAGRFACAGCGEGYHDVSKTPRVPGRCDRCGSTEFTR
                     RPDDNESVVRARLKAYHDQTEPLIHYYTRQGKVRTVDGMADIGTVSKEIGRAIDDK"
     gene            14231..14599
                     /gene="rpsM"
                     /locus_tag="VFY74_07770"
     CDS             14231..14599
                     /gene="rpsM"
                     /locus_tag="VFY74_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5931629.1"
                     /translation="MARIAGVNIPTNKRVEVALTYIHGIGDTFARQICGKVAIPPERR
                     VSQLTESEIMQIREVIDHDFVVEGDLRRDVAMNIKRLMDLGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPAKPIAGKKK"
     gene            14665..15054
                     /gene="rpsK"
                     /locus_tag="VFY74_07775"
     CDS             14665..15054
                     /gene="rpsK"
                     /locus_tag="VFY74_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5931630.1"
                     /translation="MAKDKTRVRRREKKNITSGVAHVHASFNNTMITITDAQGNAISW
                     SSAGTMGFKGSRKSTPYAAQVAAEDAGKKAMEHGMKNLEIEVRGPGSGRESALRALQA
                     AGFNIISIRDVTPIPHNGCRPRKRRRV"
     gene            15147..16163
                     /locus_tag="VFY74_07780"
     CDS             15147..16163
                     /locus_tag="VFY74_07780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5931631.1"
                     /translation="MLQKNWQDLIKPTKLNVVPGREPARLATVVADPLERGFGLTLGN
                     ALRRVLLSSLQGAAITSVHIDGVLHEFSSIPGVREDVTDIVLNIKEIALRQHAEGVKR
                     MVLKKEGPGVARAGDIEAGADIEILNPDHVICTLDEGASIRMEFTTSTGKGYVPAERN
                     RPDDAPIGFIPVDALYSPVRKVSYRVENTREGQILDYDKLTMEIETDGSVRPEDTVAL
                     AARIVQDQLSVFVNFEEPKKETTFERQPELVFNAALLKKVDELELSVRSANCLKNDNI
                     VYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPDWPPENIEELAKRF
                     EDHY"
     gene            16275..16703
                     /gene="rplQ"
                     /locus_tag="VFY74_07785"
     CDS             16275..16703
                     /gene="rplQ"
                     /locus_tag="VFY74_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5931632.1"
                     /translation="MRHRNQGRKLNRTPTHRSALLASLAAALIKHEQIATTLPKAKEL
                     RRVADRLITLAKKGDLHARRLAFSRLRDEGMVAKLFDTLGPRYAARPGGYTRVLKAGF
                     RYGDSAPMALIELVDRDPAAKGLDSGPPQNEADEADKAAA"
     gene            complement(17061..17612)
                     /locus_tag="VFY74_07790"
     CDS             complement(17061..17612)
                     /locus_tag="VFY74_07790"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5931633.1"
                     /translation="MRMMKLALAAGLAVVIGSGSAYAITVKKRIEAPGQPADVWVVAG
                     EAFCTIKTWHPAVAECEETKEGDVTFRTLTLKDGGKIKEKLTGTEDMSYSYEIIESPL
                     PVKNYSSKLWVEADDEEDRSVIYWQSEFDAADGKSDEEAKKVMTGILGDGVKGIKKNA
                     LAAWDKKHPEDAPDSDDDDDNGN"
     gene            17783..19216
                     /locus_tag="VFY74_07795"
     CDS             17783..19216
                     /locus_tag="VFY74_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX5931634.1"
                     /translation="MTFLAKSAARLKATVVLALIGLLVLSVEGKAERRPPESAEALKL
                     SFAPVVKRAAPAVVNVYVRHRVTQMVSPFFNDPFFGRMFGERFGIPRERIQNSLGSGV
                     LVGSDGVVVTNYHVIQGSGEAEITVALADGREFPAKIILKDEQTDLAVLRLDADQVAF
                     PSIAFSDSDGLEVGDLVLAIGDPFGVGQTVTSGIVSALARTQVGISDYQFFIQTDAAI
                     NPGNSGGALVDMDGRLVGINTAIFSRSGGSHGIGFAIPSNMVRLVVQSALTGGKVQRP
                     WFGAALQALTPDIADTLGLEVPAGALIAKVHAKGPAAKAGLRAGDVVVSVDAKPVANP
                     GGFEYRFVTKGVGGSAELDVVRKGQRVKATIVLVAPVEDPPRDARDLLGRHPLTGCKV
                     ANLSPAVAQEIGMDDDTREGVVVLEVKERTPAARLGIKRGDIVMAVNDEKVKSVAQLS
                     DVLVASGGGWRLSVEREGKVFNLAIQG"
     gene            19220..20551
                     /locus_tag="VFY74_07800"
     CDS             19220..20551
                     /locus_tag="VFY74_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5931635.1"
                     /translation="MKKAAATLFEAAGLAEAAPRPLADRLRPASLAEVIGQDHLLGEQ
                     GMLSRMVKAGRLSSVIFWGPPGTGKTTVARLLAEVTDLHFEQLSAIHSGVQDLRKVFD
                     AAKARREEGRGTLLFVDEIHRFNRSQQDAFLPHMEDGTIILVGATTENPSFELNSALL
                     SRASVLTFRRLDEGAIETLLQRAEAHAGRKLPLTPDARDALINMADGDGRGAINLAED
                     IFLAEDAGAQVPLDRGALIELVQRRAPIYDKSQDGHYNLISALHKSVRGSDPDAALYW
                     LARMLAGGEDPLYIARRLVRMAVEDIGLADPNALVQAIAAKDTFDFLGSPEGELALAQ
                     ATIYLATAPKSNAAYVAFGAAQRTAEEGGSLLPPKHILNAPTRLMKQEGYGRGYAYDH
                     NEDEAFSGQNYFPDGLARQNFYAPTSYGFEAELKARLDRFAALRKKRQAPR"
     gene            20634..>21273
                     /locus_tag="VFY74_07805"
     CDS             20634..>21273
                     /locus_tag="VFY74_07805"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5931636.1"
                     /translation="MAGVETKDVARGEDGMRLDRWFKTHYPKLPHSRLEKLLRTGQVR
                     VDGGRAKSSTRLAEGQKVRVPPLPDVAPPPRHHLSKSDRDFLASMTLYEDDDLLILDK
                     PSGIAVQGGTKTPHHIDRLLEGLGDGPETRPRLVHRLDRDTSGVLVVAKRRAVAAKLG
                     RAFQTRSVRKIYWALVHGVPKPPQGKIEAALVKAAGPDGDRVRKARPGEQEVA"
BASE COUNT         4263 a         6657 c         6966 g         3387 t
ORIGIN      
        1 tttcccgagc ccgtcatcga ggtcgccgtc gagccgaaga ccaagggcga ccaggagaag
       61 ctcggcgtgg cactcaaccg cctcgcccag gaggatccct cattccgcgt cgccgtcgat
      121 cacgaatccg gccagaccat catcaagggc atgggcgagc ttcatctcga catcatcgtc
      181 gaccgcatgc ggcgcgagtt caaggtcgag gccaatgtcg gcgcgccgca agtggcgttc
      241 cgcgagacgg tctccaagcg cgccgatgtc gattacaccc acaagaagca gaccggcggc
      301 tcgggccagt tcgcgcgcgt caagatgacg atcgagccga acgagcaagg caagggctac
      361 gagttcgaga acaaggtggt cggcggcaac gtgccgaagg aatatatccc cggcgttcag
      421 aagggcgtgc agagcgtcgt ggaatccggc gtgcttgccg gcttccccat cctcgacgtc
      481 aaggtgacgc tgaccgacgg cgcctatcac gatgtcgatt cgagcgtgat ggcgttcgag
      541 atcgccggcc gcgccgcctt ccgggaaggg gcgctgaagg cgggaccgaa gctgctcgag
      601 ccgatcatga aggtcgaggt ggtgacgccg gaggattatg tcggcggcat catcggcgat
      661 ctcaccagcc ggcgtggaca ggtgcgcggc caggagacgc gcggcaacgc cgcggtgatc
      721 aacgccatgg tgccgctcgc gaacatgttc ggctacgtga acaatctgcg ctcgatgagc
      781 cagggccgcg cgcagttcac gatgcagttc gatcactacg cgcaagcgcc ccagggcgtt
      841 gccgaggagg tgaaggcgaa attcgcctag acggtcttgg cgcccgccag gcgtccggat
      901 gagttttatt ttgtgttgaa ggatggagag ccagcatggc taaggaaaaa ttccagcgga
      961 cgaagccgca ctgcaacatc gggaccatcg gtcatgtcga tcacggcaag acgacgttga
     1021 cggccgcgat caccaaggta ttggcagaaa agggcggcgc gaaattcacc gcctatgacg
     1081 agatcgacaa ggcgccggaa gagaaggcgc gcggcatcac catttcgacc gcccacgtcg
     1141 agtacgaaac cgacaagcgg cactacgccc atgtcgactg tccgggccat gccgactacg
     1201 tcaagaacat gatcaccggc gccgcccaga tggacggcgc catcctcgta gtctcggcgg
     1261 ccgacgggcc gatgccgcag acccgcgagc atatcctgct cgcccgccag gtgggcgtgc
     1321 cggcgctggt cgtctatctc aacaaggtgg acatggtcga cgatccggag ctgctcgacc
     1381 tcgtcgagct cgaggtccgc gagctcctgt ccaaatacga gtttccgggc gacgagatcc
     1441 ccatcgtcaa gggctcggcg ctggctgcgc tcgaaggcaa ggacgagcag gtcggcaaga
     1501 cctcggtcgt cgaactgatg aaggcggtcg acgattacat cccgcagccg gaccgtccca
     1561 aggacaagcc gttcctgatg ccgatcgagg acgtgttctc gatctcgggt cgcggcacgg
     1621 tggtcaccgg acgcatcgag cgcggcatca tcaaggttgg cgaagaggtg gagatcgtcg
     1681 ggatcaagcc gaccaccaag accacggtca ccggcgtcga gatgtttcgc aagctgctcg
     1741 atcagggcga ggccggcgat aatgtcggct gcctgttgcg cggcatcgat cgcgaggcgg
     1801 tggagcgggg tcaggtgctg gcgaagcccg gctcggtcac gccccacacc aagttcaaag
     1861 ccgaggcgta tatcctcacc aaggaggagg gcgggcgcca cacaccgttc ttcggcaatt
     1921 accgtcctca gttctacttc cgcaccaccg acgtgaccgg cgtcgtgcat ctgccggaag
     1981 gcaccgagat ggtgatgccg ggcgacaata tcgccatgac ggtcgagctg attgtgccga
     2041 tcgccatgga ggaaaagctg cgtttcgcca ttcgcgaggg cggtcgcacc gtcggtgcgg
     2101 gcgtcgtggc atcgatcatc gagtagagag acgaaaggag ggcgccgcct gaaaacggcg
     2161 cgctcctgaa tttgaggcca tcgagatgca aagtcagaac ataagaatca ggctcaaggc
     2221 cttcgaccat cgcatcctcg atgcgtcgac caaggagatc gtgaacacgg cgaaacggac
     2281 gggcgcggaa gtgcgcggcc ccattccgtt gccgacccgt atcgagaagt tcaccgtgaa
     2341 tcgctcgccg catatcgaca agaagagccg cgagcagttc gaaatgcgca cccataagcg
     2401 gctgctcgac atcgtcgatc cgacgccgca gacggtcgac gcgctgatga agctcgacct
     2461 cgccgccggc gtcgatgtcg agatcaagtt gtaacggcac gcggcgaggc aaggactaaa
     2521 gaccgatgcg atcaggtgtc atcgcgcaaa aggtcggcat gacccgaatc ttcaccgatg
     2581 ccggagagca cattccggtg acggtgctgc gcctcgagca ttgccaggtg gtgggccacc
     2641 ggacctccga gaagaacggc tataccgccc tccagctcgg cgccgggcgt cccaaggtga
     2701 agcgcttgag ccgcgcggcg cgcggtcact tcgccgtcgc caatgtcgag ccgaagcgca
     2761 agctcgccga gtttcgcgtg agccccgaca acctgatcga ggtgggggcc gagatcaccg
     2821 ccgaccattt cgtcgagggc caatacgtgg acgtgtcagg cacctcgatc gggaagggct
     2881 tcgccggcgc gatgaagcgc cacggctttg ccggcttgcg cgcgagccac ggcgtgtccg
     2941 tgagccaccg cagccacggc tcgaccggtc agcgccagga cccgggcaag gtgttcaagg
     3001 gcaagaagat ggccggccac atgggcgacg tgaacgtcac cacgcagaac ttgcgcgtgg
     3061 tgaagaccga tcctgagcgc gggctgatca tgatccgcgg cgccgtgccc ggggccaaag
     3121 gcggctgggt gctgctgcgc gacgcggtca agcgcgcatt gccggaagcg gcgccgaagc
     3181 ccggcgcgtt ccgcaagagc tatcagccgg agcaggcgaa gaaggccaag gccaggaaga
     3241 ccaagagtgc cgagagcgcc gaaagcgccg catccgaggc gggggaaggc gggagccagg
     3301 agtcatgaag gccgacgtca ccactctcga cgccaagaag gcgggcaccg tcgagcttcc
     3361 cgacgacctg ttcgggctcg agccgcgtcc cgacatcttg cagcgcatgg tgcgctacca
     3421 gctcgcgaag cgccggagcg gcaccgctca cgtgaaggat cgctcggaga tcaacgcgac
     3481 gaccgccaag ctctatcgcc agaagggcac gggccgcgcg cgtcacggca gcaagaaggc
     3541 gccgatcttc agaggcggcg gcaaggcgtt cggacctaag cccagaagct ttgccgtcgg
     3601 cctgccgaaa aaggtgaggg cgcttgcgct gaaacatgcg ctgtcggcca aggccaaatc
     3661 cgacacgctc atcgtcatcg acaaatgcga gctgaaggac ggcaagacca aggagctcaa
     3721 gtcgcgcttc gccaagctcg gcttcggctc ggtgctgatc atcgacgggg cctcggtgca
     3781 ggaggccttc gccaaggccg cgcgcaatct gcccgaggtc gacgtgctcc cggtgcaagg
     3841 catcaatgtc tacgacattc tccggcacga gaagctggtg ttgaccaagg ccgcgatcga
     3901 ggcgctggag gcgcggttca aatgaccacc cgcaccgact atgacatcat ccttgcgccg
     3961 gtcatcaccg agaaggcgac agccgtttcg gaggccaatc aggtcgtgtt caaggtccgc
     4021 cgcgacgcga ccaagccgga gatcaaggcg gccgtcgaga cgctgttcaa ggtcaaggtg
     4081 ctgtcggtga acacgctcac ccgcaaaggc aaggcgaaga acttccgcgg cgtcaggggc
     4141 agacagcagg acgtcaagaa agcgatcgtc aggctcgccg aaggccagac gatcgacgtg
     4201 acgacgagga tctgacggga tggcgctcaa gacctataaa ccgaccacgc cgtcgcaacg
     4261 gcagctcgtg ctgatcgacc gcagccagct gtggaagggt gcacccgtca aggcgctcac
     4321 caaagggctt agcaagaccg gcggccgcaa caatcacggt cgcgtgtcga tgtggcaccg
     4381 gggcggcggg cacaagcgcg cctatcgcaa ggtcgacttc aagcgcggca aactcggcgt
     4441 gcccgccacg gtcgagcggt tggaatatga cccgaaccgc actgccttca tcgccctcat
     4501 caaatatgaa gacggcgagt tgtcctacat tctcgcgccg cagaggctcg ccgtgggcga
     4561 taccgtgatt tccgatctcc gcgccgacgt gaagcccggc aacgccatgc cgctcgccaa
     4621 catgccgatt ggaaccatca tccacaatgt ggagatgaag aagggcaaag gcgggcagat
     4681 cgcgcgcgcc gccggcacct atgtgcagct tgtcggccgc gacgcgggct acgccattct
     4741 caaactgaat tccggcgaga cgcgtatggt gccgtccgag tgcatggcga gcgtcggcgc
     4801 cgtctcaaat cccgacaatt ccaacatctc gatcggcaaa gccgggcgcg cacgctggaa
     4861 gggccgcaag cccgtggtgc gcggcgtcgc catgaatccg atcgaccatc cgcatggcgg
     4921 cggcgagggc cgcacctccg gcggtcgcca cccggtatcg ccctggggca agtccgcgaa
     4981 gggcaagaag acccgctcga acaagtcgag caacaagttc atcctgcgca gccgccacca
     5041 gaagaaggga tagttcacgt gggacgctcg atctggaaag gcccgttcgt cgacggatat
     5101 ctgctcaaga aggccgagaa ggcgcgcggc tctggctcga acgcggtgat caagatgtgg
     5161 tcgcgccgct cgacgatcct gccgcaattc gttggcctga cgttcggcgt gcataacggc
     5221 cacaagcaca tcccggtgct cgtcaccgaa gacatgatcg gtcacaaatt cggagagttc
     5281 gcgccgacgc ggaccttcca cggccacgcc gccgaccgca aggcgaagag ggtgacgaag
     5341 gaagcgtaat gggcaagccg cagcgcgagc gcgcgctcaa agagaacgag gcgagagcgg
     5401 tgacgcgtct cgtgcgcgtg agcccgcaga agctcaatct gcttgcccag ctgatccgcg
     5461 gcaagaaggt cgatcgtgcg ctcgccgatc tcaccttctc gcgcaagcgc gtcgccaagg
     5521 atgtgaaaaa gacgcttcaa tcggccattg ccaacgccga gaacaatcac gatctcgatg
     5581 tcgacgctct cgtcgtggcc gaagcctatg tcggcaagaa cctggtcatg aagcgcatgc
     5641 gcgcccgggc gcgtggccgc gccggccgca tcgagaagcc attcgcgcag atcaccgtcg
     5701 tgctgcgtca agtcgaggag cccgcgtaat gggacagaag gtcaatccga tcgggcttag
     5761 gctcggcgtc aaccgcacct gggattcgcg ctggtacgcc tcgcgcggcg aatatgcgcg
     5821 gcttctgcac gaagacttga agatgcgcga gcacatcctg gagtcgcgca agcaggccgg
     5881 catctccaag gtcgtgatcg agcgcccgca caagaaatgc cgcgtgacgg tgcacacggc
     5941 gcggccgggc atcctcatcg gcaagaaggg cgccgatatc gagaagctca ggcgcgagct
     6001 agccaacatg acggactccg aggtgcacct caatatcgtc gaggtgcgca agcccgagat
     6061 cgacgcgacc ctcgtggccg aaggcatcgc ccagcagctc gagcggcggg tggcattccg
     6121 ccgcgccatg aagcgagccg tgcagtcggc gatgcgcatg ggcgcgctcg gcatccgcat
     6181 caattgcggg gggcggctcg gcggggccga gatcgcgcgc atggagtggt atcgcgaggg
     6241 ccgtgtgccg ctgcatacgc ttcgcgccaa tatcgattac ggcacggcgc tcggtcgcac
     6301 ggcctacggg atcatcgggg tcaaggtctg gatcttcaag ggcgagatca tggagcacga
     6361 tcccatggcc caggagaaca aggcgctcga ggcccaggaa ggcggtcgct ccacggggcg
     6421 ccgcgagccg gcgcaagcgc aaggttaggc taggacttag atcatgctgc aaccgaagcg
     6481 gaccaaatac cgcaaggctc acaaggggcg catcaagggt gcggcttcga gcgcggccac
     6541 gctcaatttc gggacctacg gactcaaggc ccaagagccg gcccgcatca ccgcccggca
     6601 gatcgaagcg gcgcgccggg ccatgacaag gcagatgaag cgttcggggc gggtgtggat
     6661 caggctgtac ccggacgtgc cggtatcgaa gaagccgacc gaggtgcgca tgggcaaggg
     6721 caagggcacg cccgaatttt gggtcgcgcg ggtcaagccc ggccgcatca tgttcgagat
     6781 cgacggcgtc accgagcagg tcgcgcgcga gtcgctccgg ctcgcggctg ccaagcttcc
     6841 gatcaagacg cgcatggttg cgcgcatcga ggtcaagcca tgaaggcgca gcaggtccgc
     6901 gggcttacgc ccgatcagcg cgaagatgag ctggtcaagc tgaagaagga gcagttcaac
     6961 ctgcgctttc aggcggcgtc aggccagctc gagaacaccg cgcgggtgag acagatcagg
     7021 cgcgacatcg cccggatcaa tacgattgcg cggcaagagc cggcagccga ggctccagcg
     7081 aagcaggcgc ccgcgccaaa ggcgaagccc aagaccgcgg cgcccaaggc cgaaaaatcc
     7141 aagaccgcgg cgcccaaggc cgcgaaatcc aagaccgcgg cgagagccaa ggcaaagccc
     7201 aagacgaagg cgaagagcta ggacaagacg atgccgaaac gcgttctgca aggagtggtg
     7261 gtgagcgaca agaacgacaa gaccgtggtg gtccgcgtcg aacggcgctt cacgcaccct
     7321 gtgctgcaaa agaccgtgcg gcggagcaag aactatcagg cccatgacga gaataacgag
     7381 ttcaaggtgg gcgaaatggt gtggatcgag gaatgcccgc cggtctctaa gcagaagcgg
     7441 tggacggtct tgccgcgcga gcaggcagca cggtagcggc agtttaggtt tgaaggcgaa
     7501 agcgccatga tacagatgca gaccaatctc gacgtcgccg acaattcggg tgcgcggcgc
     7561 gtccagtgca tcaaggtgct cggcggctcg aagcgcaaat acgcctcgat cggcgacacg
     7621 atcgtggtct cggtcaagga cgcgataccg cgcggccgcg tgaagaaggg ccaggtgatg
     7681 aaggccgtgg tggtccgcac cgccaagggc gtgcgccggc ccgacggctc gatcatccgc
     7741 ttcgatcgca acgccgcggt cctgatcaac ccgcaaggcg agccgatcgg cacccgcatc
     7801 ttcggcccgg tcacccgcga gctcagagcg aagaagcaca tgaagatcgt ttcgctcgcg
     7861 ccggaggtct tgtgatggcg aagctcaaga tcaagaaagg cgaccatgtc gtcgtcatca
     7921 ccggcaagga caaaggcaag aagggcgagg tcctgaaggt catgcccgag gagaaccgcg
     7981 ccatcgtcaa gggcgtggcc atggtgcgcc gccaccagcg ccagtcgccg tcgcaggagg
     8041 gcgggatcat cgccaaggag gcgccgatcc acatctccaa tctcgcgctg gaggacccca
     8101 aggacgggaa cccgacgcgc gtcgggtaca aattcctgaa ggacgggcgc aaggtccggt
     8161 tcgcaaggcg ctcgggcgag gtcatcgatg gctgagacga cctatatccc ccggctcaag
     8221 cagcactaca acgaggtgct caagccagcg ctcgccaagg agttcggcta caccaacgcg
     8281 atgcaggtgc cgcgcctgga gaaggtggtg ctcaatatgg gcgtgggcga gggcgtcagc
     8341 gactccaaga aggttcaggc cgccgccgcc gatctcggga tgatcgccgg gcagaagccg
     8401 gtgatcacgc gggcgcggaa atcgatcgcg acgtacaagc tccgcgaggg catgtccatt
     8461 ggcgccaagg tcaccctgcg gcagaatcgc atgtacgagt tcgtcgaccg gctggtgaac
     8521 atcgcactgc cgcgggtgcg ggacttccgc gggctcaatc cgaagagctt tgacgggcgc
     8581 ggcaatttcg ccctcggact gaaggagcac atcatcttcc ccgagatcga ctacgacaag
     8641 gtggaagacg tgctcggcat ggacatcatc gtttgcacga cggcgcgcac cgacgacgag
     8701 gcgcgcgcgc tgcttaaggg tttgaacttt ccgttccgca gctgagccaa ggctcgggac
     8761 tgcgacagga ggacttgaat ggcaaagacg agcacaatcg agcgggacaa gaagcgccgc
     8821 cgcctcgcca agaagtacaa gctcaggcgg acgcggctga aagcgattgc cgccgaccgg
     8881 tctctgccgg ccgaggagcg gttcgcggcc cggctcaagc tcgcagagct gccgcgcaat
     8941 tcgtcgccca cgcgtatccg caaccgttgc gatctcaccg ggcgcccccg cggctattac
     9001 cgcaagctgc gcatgtctcg gatcgcgctg cgcgacctcg gaagccaggg actcgttccc
     9061 ggcatgatca agtcgagctg gtaggacgag cgaatgagca tcaacgaccc actaggcgat
     9121 atgctggccc gcatccgcaa cgcgcagatg cggcacaaga ccacggtgac gacgccggcc
     9181 tccaagctca gagagcgtgt gctcgatgtg ctggccgagg agggctatat ccgcggctat
     9241 gcgcgcgtcg actatagcgg cggccggtcg gagttcgaga tcgagctcaa atatttcgac
     9301 ggcgagcctg tgatcaagga tatcaagagg gtgtcgacgc ccggacggcg cgtctattcc
     9361 tcggtcaagg atttgcccac ggtcgccaac ggtctcggcg tcgccattct ttcgacgccg
     9421 aaaggcgtga tgtcggatgc gcgggcgagg accgagaatg tcggcggcga aattttgtgt
     9481 aacgtgttct agggtcacga gctcggacga aggttgattc atgtcgcgca tcggcaagaa
     9541 gcccgtttca gttcccggcg gcgtcaccgc cgcggtgaac ggccaagagg tcaagatcaa
     9601 gggccccaag ggcgagctca agcacgtgct cgccgacgcc atggtggcaa agctggacaa
     9661 ggacggcatc gagatcacga tgcgggagga cacgaaagaa gcccgcgcca tgtggggcat
     9721 gtcgcggacg ctcatcgcca atctcatcgc cggggtgacg gaaggctttt ccaagcggct
     9781 cgagatctcg ggcgtcggct accgcgcggc ggtgcagggc tcaaatctcc agcttcagct
     9841 cggctatagc cacgatgttg cctatccgat cccgcaaggt atccagatcg catgcccgaa
     9901 gccgacggag atcgtgatta cgggcatcga taagcagaaa gtgggtcagg ttgccgccga
     9961 gatcaggcgg ttcaggccgc ccgagcccta taagggcaaa ggcatcaaat atgcgggcga
    10021 gttcatcctc cgcaaggaag gcaagaagaa gtagcgggac gatcggaaat gggcgcgttg
    10081 aacgaaagct tcatgcggcg ctcgcgccgg gttcgccata cgctcaagcg tgcggcgaac
    10141 gggcgcgcgc gcctgtcggt gttccgctcc tcgcggcata tctacgcgca agtgatcgac
    10201 gatgaggcgg gcaagacgct ggcggcggcc tcgacgctcg acaaggacct gaagggtgcg
    10261 ctgccgaagg gcagcgatct cgcggccgct caggccgtcg gcaagctcct ggcggaaaga
    10321 gcgttgaagg ccggcgtcaa ggacgtcgtg ttcgaccgcg gcggttatat ctatcatggg
    10381 cgcgtgaagg cgctcgccga cgccgcgcgc gaagcaggcc tgaacttcta gtttcgacct
    10441 aagaaaggat cgaccgtggc acgaggccag caagcaagag accgtgaaga gcgcgatcgt
    10501 gacagcgaat tcatcgacaa gctcgtgcac atcaatcgcg tggccaaggt ggtcaagggc
    10561 ggacggcgct tcggcttcgc cgcgctggtc gtggtgggcg atcagaaggg acgggtcggc
    10621 ttcggacacg gcaaggcgcg cgaggtgccg gaagccatcc gcaaggcgac cgaggccgcc
    10681 aagcgcaacc tggtcaaggt tcctttgcgc gagggacgca cgctgcatca cgacgtgacc
    10741 ggccagcatg gcgcgggacg ggtcgtgctc agggcggcac ctcccggcac cggcattatc
    10801 gccggcggcc cgatgcgggc cgtgttcgag acgctcggca ttcaagacgt ggtcgcgaag
    10861 tcgctcggaa gctcaaaccc ctacaacatg gtgcgggcga ccttcgatgc gttgcggcac
    10921 gaggacagcc cgcgcggcgt ggcggcgcgc cgcggcaaga aggtgagcga aatcgtctcg
    10981 cggcggagag acggcgccgg cgacgccgcg ggcgctccgg aagcctaagc ctcaagagag
    11041 cgcgtcggat aaagggtttg ccacatggcc gagaaaagaa agaccatcgt cgttgagcag
    11101 atcgggagcc cgatcaggcg ccccgcgagc cagcgcgcga cgctgatcgg gctcggcctc
    11161 aacaagatgc ataagcggcg gacgctcgtc gacagcccgc aggtgcgcgg aatggtggcg
    11221 aaggtcagcc acctcgtccg gatcgtcgat gacgagccga aggcttaagg aagagacgtc
    11281 atgcgattga atgcattaac cgaccggccg ggcgcgacga gggccaagaa gcggctcggc
    11341 cgcggtattg gctcgggcct cggcaagacc gcgggcaagg gcaccaaggg ccagaaggcc
    11401 cgcgccggcg tcgccatgaa gggcttcgag ggggggcaga tgccgctgca ccggcgcctg
    11461 ccgaagcgcg gcttcaacaa catcttcgcc aggaaattca acgagctcaa tctcggccgc
    11521 atccaagagg cggtggatgc gggcctcctt gacggcaaga agccgatcac cgttgcgtcc
    11581 ttgaaggagg ccggtctcat ccggcgcgag aaggatggcg tgcgtctgct cggtcatggg
    11641 gaactcaagg caaagctcgc cttcgaggtc accggcgcct cgcaaagcgc gatcaaggcg
    11701 gtcgaagcca aaggcggcac ggtgacactc aaatccatta ccggacgcga gcgtcctgcc
    11761 gccgacaagc tcaaggccga gcgcagggca gcaaaacgcg ccgccaaggc agcgccaaag
    11821 ggcggcaagg ccaaggccaa ggaggctcag gcggcggaag gcacgaaggc ggcttcggac
    11881 gaagcgcccg ccaaagccaa gcctgcgccc gagactgagg ccgcgagcag cgccaagaag
    11941 tcgaagccgg ccgagggcga ggccaagacg ctgcaggcca agccagccaa gaaggacaag
    12001 tccgagtgag gcggggcgcg gcggcaccgc cgcctcctcg ctaaatgaag agtgtttcga
    12061 tggtttcagc cgcagagcag ctcgtttcca atctcaactt cgccgccttc gccaaggccg
    12121 acgagctcaa gaagcgcatc tggttcacgc ttggcgcgct cctgatctat cggctcggaa
    12181 cctatattcc gatccccggc atcaatcccg aggcgcttgc cgatgtcttc cggcagcagc
    12241 aatccggcat cctcggcatg ttcaacatgt tgtcgggcgg cgccatcggg cgcatggcca
    12301 tcttcgccct caacatcatg ccgtatatct cggcgtcgat tatcatccag ctgctcacca
    12361 ccgtctcgcc gcatctcgag cagctcaaga aagagggcga gcagggacgc cggcagatca
    12421 accaatacac gcgctacggc accgtgctgc ttgccgcgct gcagggctac ggcatcgcca
    12481 tcggcttgga gggcgccggc aacgtcgtgg tcgatcccgg ctggtttttc cgcatcacca
    12541 cggtcatcac gttggtcgga ggaacggtct ttttgatgtg gctcggcgag cagatcaccg
    12601 cccgcggcgt cggcaatgga atctcgctta tcatcttcgc tggcatcgtg gccgaattgc
    12661 cctcggccct catcggcacg ctcgaactcg ggcgccaggg cgcgctgtcg acggcgttga
    12721 tcatcggcct cctgggcatg gcggtgctcg tgatcgcctt tatcgtcttt atggaaaggg
    12781 cgcagcggcg gctgctggtg cagtatccga agcgccagat cggcaaccgc atgttccagg
    12841 gcgattcctc gcacctgccg ctcaagctca atagcgccgg cgtgatcccg ccgatcttcg
    12901 cgtcctccct tttgctcctg cccatcacct tggcgaattt ctccgccggt cagggtccgg
    12961 attggctcaa cagcatcacc gccatgctcg gaatgggcca gccgcttcac atcgtcctct
    13021 acgtggcgat gatcatcttc ttcgccttct tctatacggc ggtcgtgttc aatccgaagg
    13081 acacggcgga gaacctgaag aagtatggcg gatttctccc tggaatcagg cccggcgagc
    13141 gtacggcgga atatatcgac tatgtgctca cccgcatcac cgtcgtcggc gcgctctacc
    13201 tcgccgccgt ctgcgtgctg ccggagctcc tgatctcata cacgggcgtg ccgttctact
    13261 tcggcggcac ctcgctcctg atcgtggtga gcgtcaccat ggatacggtc gcgcagatcc
    13321 agagccatct gctcgcccat cagtatgagg gcctgatcaa gcgggcgcgg ctcaaagggg
    13381 cgaggcggcg atgaacctgg tcctactcgg ccctcccggc gcgggcaaag gaacgcaggc
    13441 gaagctcctg gaagaatccc ggtcgctggt caagctctcg accggcgaca tgcttcgcgc
    13501 cgccgttgcc gccggcaccg agcttggccg caaggccggc gacatcatgg aacgcgggca
    13561 actcgtgccc gacgtgctcg tcatcaattt gatcgcggag cgcctggacc aggacggggg
    13621 cgggcagggc ttcatccttg acggattccc ccgcaacatc gcccaggccg aggcgctcga
    13681 ccggctcttc aaggagcgcg gcaaggcggt cgaccgggtc atcgtgatgg atgtcgatga
    13741 cgaggcactg gtcgagcgta ttgccggccg ctttgcctgc gccggttgtg gagaggggta
    13801 ccacgacgtc tccaagacac cccgcgtgcc cggacgctgc gaccgctgcg ggagcaccga
    13861 gttcacccgg cgccctgacg ataatgagtc ggtcgttcgc gcgcggctca aggcgtatca
    13921 cgaccagacc gaaccgctga ttcactatta cacgcgtcaa ggtaaagtca ggaccgtcga
    13981 cggaatggcc gatattggaa cagtttccaa ggaaatcggc cgtgcgatcg acgataaatg
    14041 aggaaaaaac gggcaaaaac ggccggttga ccttgtcaag cggaatcgct acataagccc
    14101 cgctaacgga aagatggaac tgaccggtcc ccggggggca gcgcctgtcc gcggtcggtc
    14161 ttgttgtgtt tgagattggc tattctggct tagatttcag cacgttagcc gtgcacgagg
    14221 agacgactac gtggcccgaa ttgcaggcgt gaacatcccg accaacaagc gggtcgaggt
    14281 ggctctgacg tatatccatg gcatcggcga taccttcgcc aggcaaattt gcggcaaggt
    14341 cgccattcct ccggagcggc gcgtcagcca gttgaccgaa tcggagatca tgcaaatccg
    14401 cgaggtcatt gaccacgact tcgtggtgga aggcgatctc cggcgcgacg tcgccatgaa
    14461 tatcaagcgg ctgatggatc tcggctgcta ccggggctta cgccatcgcc gcggactccc
    14521 cgtgcgcgga caacgcactc acaccaacgc gcgcacgcgc aaaggcccgg ccaagccaat
    14581 cgccggcaag aagaaatagg tcttaggcaa gcggcgcgag ctccgctcgc gtccggcgtc
    14641 aattcggaat tgcgggatta gagcatggcc aaggacaaga cgcgggttcg tcgccgcgag
    14701 aaaaagaaca tcacctcggg cgttgcccac gtgcatgcaa gcttcaacaa cacgatgatc
    14761 accatcaccg acgcgcaagg caacgccatc tcctggtcgt cggccggcac gatgggtttc
    14821 aagggatcgc gcaagtccac tccttacgcc gcgcaggtcg ccgccgagga cgccggcaag
    14881 aaggcgatgg aacacggcat gaagaacctc gagatcgagg tcagggggcc aggatcggga
    14941 cgcgagtcgg cgcttcgagc gctccaggcc gcaggcttca atatcatctc gatccgcgac
    15001 gtgacgccga taccgcataa cgggtgccgg ccgcgcaagc gccgtcgcgt ctaacggaaa
    15061 ggcaggctag gccgcaaatg ttgcatgaag attctcggaa gctggggcgg accgccccgc
    15121 tcatcgacca agaaagggct tcaccggtgc tgcagaagaa ctggcaagat ctcatcaagc
    15181 ccaccaagct caacgtcgtt cccggccgcg agccagcgcg tttggcgacg gtcgtggccg
    15241 atccgctcga gcgcggtttc gggctcacgc tcggcaatgc gctccgccgc gtgttgttgt
    15301 cctcgctgca aggcgccgcc atcacttcgg tgcatatcga cggcgtgctg cacgagtttt
    15361 cgtccatccc cggcgtgcgc gaggacgtga ccgatatcgt cctcaacatc aaggaaatcg
    15421 cgctcaggca gcatgccgaa ggcgtgaagc gcatggtgct caagaaggaa ggccccggcg
    15481 tcgctcgcgc cggcgacatc gaggccggtg ccgatatcga gattctcaat cccgatcacg
    15541 tcatctgcac gctcgacgaa ggcgcctcta tccgcatgga gttcaccacc tcgaccggca
    15601 agggctatgt gccggcggaa cgcaatcgtc ccgacgacgc gccgatcggc ttcattcccg
    15661 tcgacgcgct ctacagcccc gtgcgcaagg tgtcgtaccg cgtcgagaac acccgcgagg
    15721 gccagatcct cgactacgac aagctgacca tggagatcga gaccgacggt tcggtcaggc
    15781 ccgaagatac cgtcgcgctc gccgcgcgga tcgtgcagga ccagctctcg gtgttcgtca
    15841 atttcgagga gccgaagaag gaaacgacct tcgagcggca gcccgagctc gtgttcaacg
    15901 ccgcgctgct caagaaggtc gacgaactcg aactgtcggt ccgctcggcc aattgtctca
    15961 agaacgacaa catcgtctat atcggcgatc tcatccagaa gaccgaggcc gagatgctgc
    16021 gaacgccgaa tttcggccgg aagtcgctga acgagatcaa ggaagtgctg gcctcgatgg
    16081 ggctccacct cggcatggaa gtgccggact ggccgccgga gaacatcgaa gagctggcca
    16141 agcgcttcga ggatcactac taaacaggta acagagatca gaaatcagaa atcagatttt
    16201 tctaaatcag aaatcagatt tttctgatga ctgatcactg atatctgacc cctgaaaacc
    16261 gaaggttttc gcaaatgcgc caccgcaacc agggccgtaa gctcaatcgc acccccacgc
    16321 atcgcagtgc gttgcttgcc agtctcgccg cggcgctgat caagcacgag cagatcgcca
    16381 cgaccttacc caaggccaag gagctccggc gcgtagccga ccggctgatt acgctggcca
    16441 agaagggcga tctccacgcg cgccgtctgg ccttttcccg gctccgggac gaaggcatgg
    16501 tggcgaagct attcgacacg ctcggtcccc gctatgcggc ccggcccggc ggctatacgc
    16561 gggtgctcaa ggccggtttc cgttatggcg actctgcgcc gatggcgctg atcgagcttg
    16621 tcgatcgcga ccccgcggcc aagggcctcg attccggacc gccgcaaaac gaggccgacg
    16681 aagccgacaa ggccgccgcc tagtcgcaag gccgaaccgc tcgcgggtgc gagaagactc
    16741 aaatcttgat gtaaggcaaa atgaacaggg tgcatcgctt gagcgaagcg cccttgcggg
    16801 cgcatctcag catgacggcg ttggtgtcgt cgtcacgggc attgcctttt tccgctctcc
    16861 ccttggggag agcacgcgag cgcattatcg cgctcgcggt gaggggtaag ccgcgaagcg
    16921 gcgtcgaaaa catgaacccc ctcacccttc ccgccatgcg acttgcgcag agctacgctc
    16981 acgcaagatc gcggcggaaa gccctctccc tcaagggaga gggggaaact aggtccgtgc
    17041 tccgagccgc ccattatgtc ttagttgccg ttgtcgtcgt cgtcatcgct gtcgggcgcg
    17101 tcttccgggt gcttcttgtc ccaggcggcg agggcgttct tcttgatgcc cttgacgccg
    17161 tccccgagaa ttcccgtcat caccttcttt gcctcttcgt cgcttttccc atcggcggcg
    17221 tcgaactcgg actgccaata gatcaccgaa cggtcctcct cgtcgtccgc ttcgacccag
    17281 agtttcgacg agtagttctt gacggggagg gggctctcga tgatctcgta gctgtaggac
    17341 atatcctcgg tgccggtgag cttttccttg atcttgccgc cgtccttgag cgtcagcgtg
    17401 cggaacgtca cgtcgccttc cttggtctcc tcgcactcgg cgaccgcggg atgccaggtc
    17461 ttgatcgtgc aaaaggcctc gccggcgacg acccacacat cggcgggctg gccgggcgcc
    17521 tcgatcctct tcttgacggt gatggcatag gcggaaccgc tgccaatcac gacagcaaga
    17581 ccggcggcca gggccagctt catcatacgc atgcttttct ctccactctg tttcgttgac
    17641 ttcgcaggag gcgtgggggc gcatcttatt gaaaaggcga ggctgggcaa gcccttgtcg
    17701 ggccttgaaa cggcggtatt ccgcgccagt ttcatgcgtt atagaaaccg cgggcccggg
    17761 ggtgcgtccg agcgaaagcc acatgacgtt cttggcaaaa tcggctgcga ggttgaaggc
    17821 gacggtcgtt ctcgccctca tcggcctgct tgttttgtcc gtcgaaggca aggcggagcg
    17881 gaggccgccg gagagcgcgg aggcgctcaa actttcgttt gccccggtgg tgaagcgagc
    17941 ggcgccggcg gtggtgaacg tgtatgtccg ccatcgcgtc acgcagatgg tctcgccctt
    18001 cttcaacgat ccgttcttcg gccggatgtt cggcgagcgc ttcggcatcc cccgcgagcg
    18061 cattcagaac tcgctcggtt cgggcgtgct tgtcggaagc gacggggtcg tggtcaccaa
    18121 ctatcacgtc attcagggaa gcggggaggc ggagatcacc gtggcactcg ccgacggccg
    18181 ggaatttcca gcgaagatca tcctcaagga cgaacagacc gatcttgcgg tgcttcgcct
    18241 cgacgccgat caagtcgcgt tcccgtcgat cgcgttcagc gattcggacg gtctggaagt
    18301 cggcgatctt gtgctcgcca tcggcgatcc tttcggagtc ggacaaacgg tgacgagcgg
    18361 tatcgtctcg gcgcttgccc gcacgcaagt cggcatttcc gactatcagt tcttcatcca
    18421 gaccgatgcc gccatcaatc ccgggaattc cggcggtgcg ctcgtcgaca tggacggacg
    18481 gctggtcggc atcaacaccg cgatcttctc ccggagcggc ggctcgcacg gcatcggatt
    18541 tgcgatcccc tccaatatgg tgcggctcgt ggttcagtct gcgctcacgg gcggcaaggt
    18601 gcagcggccc tggttcggcg cggcgctgca agcgctcacc cccgatatcg ccgatacgct
    18661 cgggctggag gtaccggcgg gcgcgctcat cgccaaggtc catgccaagg gccccgcggc
    18721 caaggccggc ttgcgcgcgg gcgacgtcgt ggtgagcgtg gacgcaaagc cggtcgcaaa
    18781 cccggggggc tttgaatacc gcttcgtgac caaaggcgtc ggcggctcgg cggaactcga
    18841 tgtcgtgcgc aaggggcaaa gggtgaaggc cacgatcgtg ctcgtcgccc cggtcgaaga
    18901 cccgccgcgg gatgcgcgcg atcttctcgg ccgccatccg ctgaccggct gcaaggtcgc
    18961 caatctctct cccgcggtcg cgcaggagat cggcatggac gacgacacgc gcgagggcgt
    19021 cgtcgtgctg gaggtgaagg agcggacgcc ggccgcgcgg ctcggcatca agcgcggcga
    19081 catcgtgatg gccgtcaatg acgaaaaggt gaaatccgtg gcgcagctct cggatgttct
    19141 cgtcgcctcc ggcggcggct ggcggctatc ggtcgagcgg gagggaaaag tgttcaatct
    19201 cgccattcag ggctgagcga tgaagaaggc cgcggccacc ctgttcgagg ctgccggtct
    19261 cgccgaggcc gcgccacggc cgcttgcgga ccggttgcgg cccgcctcgc tcgccgaagt
    19321 gatcggccag gatcatctcc tcggcgagca gggcatgttg tcgcgcatgg tcaaggccgg
    19381 gcggctttcc tcggtgatct tctggggacc gccgggcacc ggcaagacga cggtggcgcg
    19441 gctcctcgcc gaggtcaccg atctccattt cgagcagctt tcggccatcc attccggtgt
    19501 gcaggacctg cgcaaggtgt tcgacgcggc gaaagcgcgc cgcgaggagg gacgcggcac
    19561 gctcctcttc gtcgacgaga tccaccgctt caatcggtcc cagcaggacg ccttcctgcc
    19621 gcacatggaa gacggcacga tcattctcgt cggcgccacc accgagaacc cgtccttcga
    19681 gctgaactcg gcgctgctgt cgcgggcgag cgtgctcacc ttcaggcgtc tcgacgaggg
    19741 ggccatcgag acgctgcttc agcgcgcgga agcccacgcg ggacgcaagc tgcctttgac
    19801 gccggacgcg cgcgacgcgc tcatcaacat ggccgacggc gacggccgcg gcgccatcaa
    19861 cctggccgag gatatcttct tggccgagga cgcgggcgcc caagtgccgc tcgaccgcgg
    19921 cgcgctgatc gagctcgtgc agcgaagggc gccgatctac gacaagagcc aggacggcca
    19981 ctacaatctc atcagtgcgc tgcacaaatc ggtgcgcggg tccgaccccg atgccgcgct
    20041 ctattggctg gcgcgcatgc tcgccggcgg cgaagacccg ctctatatcg cccggcggct
    20101 ggtgcggatg gcggtcgagg atatcggact tgccgatccc aatgcgctgg tgcaggcgat
    20161 cgcggccaag gacactttcg attttctcgg cagtcccgaa ggcgagctcg cgctcgcgca
    20221 agccacgatc tacctcgcca ccgcaccgaa atcgaacgcc gcctacgtcg ccttcggcgc
    20281 ggcgcagcgg acggcggaag aaggcggctc gttgttgccg ccgaaacaca tcctcaacgc
    20341 gccgacccgg ctcatgaagc aggaaggcta cggcaggggt tacgcctacg atcataacga
    20401 ggacgaggcg ttctcgggcc agaactactt ccccgacgga ttggcgcggc agaatttcta
    20461 tgcgcccacg tcttacggct tcgaggccga gctgaaggcg cgtctcgacc gcttcgccgc
    20521 gttgcgcaag aagaggcaag cgccgcggtg aagacgcttc tcttcgttgc ctcggccgga
    20581 ctctcgattg ccgcgctgtt cgcaggcctt ctcatcatgc gggctcgctg aacatggcag
    20641 gcgtcgagac gaaagacgtc gccaggggcg aagacggcat gcgcctcgat cgctggttca
    20701 agacccatta cccaaaactt ccccatagcc ggctcgaaaa gctgttgcgc acagggcagg
    20761 tgagggtcga cggcgggaga gcgaaatcga gcacgcggct tgccgagggc caaaaagtcc
    20821 gcgtgccgcc tctccccgat gtcgccccgc cgccgcgaca tcatctctcg aagtccgacc
    20881 gcgactttct cgcaagcatg accctttatg aggacgacga tctcctcatc ctcgacaagc
    20941 cgtccggaat cgcggtgcag ggtgggacca agaccccgca ccatatcgac cgactgctcg
    21001 aaggcctggg cgacgggccc gagacgcggc cgcggctcgt gcaccggctc gatcgcgaca
    21061 cctcgggcgt gctggtggtg gcgaagcgcc gcgcggtcgc ggccaagctc ggccgcgctt
    21121 tccagacccg ctccgtgcgc aagatctatt gggctctggt gcacggcgtt ccaaagccgc
    21181 cgcaaggcaa gatcgaggct gcgttggtca aggccgcggg accggatggc gaccgggtgc
    21241 gcaaagcgcg tcccggcgag caggaggtcg cgc
//
LOCUS       DASUQP010000176         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1723534, whole genome shotgun sequence.
ACCESSION   DASUQP010000176 DASUQP010000000
VERSION     DASUQP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723534"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFY74_07810"
     CDS             complement(<1..363)
                     /locus_tag="VFY74_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931637.1"
                     /translation="MAFGRHHEAGSRRIEIQEAGVEKGEVELEYRGAVHDGFPRAERE
                     AAAEGGEGGALEEEEEGEFLRQSHDFELEYGLTERLLFATTLSTDESLKGDFDLSSVE
                     VELKYEILQREGNGLGLAF"
     gene            746..2338
                     /locus_tag="VFY74_07815"
     CDS             746..2338
                     /locus_tag="VFY74_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5931638.1"
                     /translation="MRYQTKLLVAAGLLLAMSLSAAALAYWGVTRSHVYLTRSRIAHE
                     QLERQLQLSRHSQQLFKAWTDTLLTGMTDRPLGAAYLNKVIESDLSALQTLIEKELAI
                     VDDEERKAESAELARLTQIRQEFQRTLDQLGAVEQLRTRGQPDIAWQQLTDLLKGGID
                     QELNELIEVGVADETAEVIRIDADAARLLGRLERVSQIHAALAVLATLALVILLLRGL
                     RTPLAELLRGTSKLAEGDLAHRIAISGRDEFADLGRSFNRMAGDLQAHQKALQDARAN
                     LETLVEARTEELRQANESLRKIDATRRGFFADISHELRTPLTIIRGEGEIALRGANKR
                     VAEYKRSIERIVEQAKHLSVLVNDLLFIARQGAGAARLNMQPIDLGELLEKVCGDARV
                     IAHGRNVHVDFVDGAHDIVRGDPARLRQLFLVLLDNAVRYSKSKGAVKVEIGSDGGEV
                     RVRVSDRGIGIPPEDLDGVFERFRRGGSASAMNEEGLGLGLPVARAIVEAHKGRIEMA
                     SRPGEGTTVTVALPAAKAEANA"
     gene            2335..>2723
                     /locus_tag="VFY74_07820"
     CDS             2335..>2723
                     /locus_tag="VFY74_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5931639.1"
                     /translation="MNVLLVEDEPRVADFIDRGLRAENHGVTVASSGSEGVDLAKTGE
                     FDVIVLDLMLPDMHGYDVCQRLRLQGDHTPILMLTAMDALEDKIKGLKLGADDYLTKP
                     FDFDELLARIEALVRRSHSFAPSSNVL"
BASE COUNT          523 a          880 c          827 g          493 t
ORIGIN      
        1 gaatgccagc ccgagtccgt ttccttcgcg ctgaaggatc tcgtacttga gctcgacttc
       61 gaccgagctc aagtcgaagt cccctttcag cgactcgtcg gtcgagagag tagttgcgaa
      121 caggagtcgt tcggtaagac cgtattcgag ttcgaaatcg tggctttgac ggaggaactc
      181 gccctcctcc tcttcctcaa gcgctccgcc ttccccaccc tctgccgccg cttcgcgctc
      241 ggcgcgcggg aacccgtcat ggacggcgcc gcgatattcg agctcgacct cgcccttctc
      301 gaccccggcc tcctggatct caattcggcg agagccggct tcgtggtgcc tgccaaaagc
      361 caagcgaaag ccagcgcgga aaacgcggtg gacaacgcca agcgctgcgc gccgcgcagt
      421 gaagtgcgat gctgtcccaa gagccgcccc cgactttgag ccctgctaaa accctcgatt
      481 cttatggcct agcttagcaa atctccccct ggcgccaacg gcaaaactgg cttccccact
      541 ggtcatggca tcgaggctgt cgcccatgcc actctgaaaa taaccggagg aaaatcacca
      601 tggcgatcgg aagatggccc caagtatagg gaaactgcgt tatagaaaac cggccatttt
      661 ttgcggtgcg gcccggcttc gcccaagccc agcaaaagat gaccgtccag cccggccggg
      721 cgctcagagt cgttgagacc atccgatgcg ctatcagacg aagctcctcg tcgcagcggg
      781 attgcttcta gccatgtcgc tttcagcggc ggcgctcgcc tattggggcg tcacccggtc
      841 gcacgtctat ctgacgcgct cgcgcatcgc ccatgagcag cttgagcggc agcttcagct
      901 gtcccgccat agccagcagc tgttcaaggc gtggacggat acgcttctga ccggaatgac
      961 cgaccggccg ctcggcgccg cctatctcaa caaggtgatc gagtccgatc tctcggcgct
     1021 ccaaactctc attgaaaaag aactcgccat cgtcgatgac gaagaacgca aggccgaatc
     1081 cgcggagctc gcgcggctca cgcaaatcag gcaggaattc cagcgcacgc tcgaccaact
     1141 cggcgcggtc gagcagctac gcacccgcgg ccagcccgac atcgcctggc agcaattgac
     1201 cgatctgctc aaaggcggca tcgatcagga gctgaacgag ctgatcgagg tcggcgtggc
     1261 cgacgagacc gccgaggtca tcaggatcga tgccgacgcg gcccggctct tgggcaggct
     1321 cgaacgcgtg agccagatcc atgcggcgct cgccgttctc gccacgctcg cgctcgtgat
     1381 tctgttgctg cgcggcttgc gcaccccgct cgccgagctc ctgcgtggca cctcgaagct
     1441 ggccgaaggc gatctcgctc atcggatcgc catctccggt cgcgacgaat tcgccgatct
     1501 cggccgcagc ttcaatcgca tggcgggcga cctgcaagcc catcaaaagg cgctgcagga
     1561 tgcccgtgcc aatctcgaga cgctggtgga ggcccgcacc gaggaacttc gtcaggcgaa
     1621 cgagtcgctg cgaaagatcg acgcgacccg tcgcggcttc ttcgccgata tcagtcacga
     1681 gcttcgcacg ccgctcacga tcatccgcgg cgaaggcgag attgctctgc ggggggcgaa
     1741 taagcgcgtt gccgagtaca agcgctcgat cgaacggatc gtcgagcagg ccaagcatct
     1801 ctccgttctc gtcaacgacc tgctcttcat agcccggcaa ggcgcgggcg ccgcgcgcct
     1861 caacatgcag cccatcgatc tcggtgaact cttggaaaag gtttgtggcg acgcccgggt
     1921 gatcgcccat gggcggaatg tgcatgtcga cttcgtcgac ggagcgcacg acatcgtgcg
     1981 cggcgatccg gcgcggttaa ggcaactttt ccttgtgctc cttgataatg ccgtgcgcta
     2041 ctccaaatcc aaaggagccg tgaaggtgga gattggcagc gatggcggag aggtcagggt
     2101 gcgcgtctcg gaccgcggca ttggcatacc gccggaggat cttgacgggg tcttcgagcg
     2161 cttccgccgg ggcggaagcg cctcggccat gaacgaggaa ggcttaggcc tgggcctgcc
     2221 ggtggcgaga gccatcgtcg aggctcataa gggtaggatc gagatggcaa gccgtccggg
     2281 cgaaggaacc acagttacgg ttgcattgcc ggccgccaaa gcggaggcga acgcgtgaac
     2341 gtccttttgg tcgaagacga gccgcgtgtt gccgacttca tcgaccgcgg actccgggcc
     2401 gagaatcatg gcgttaccgt cgcttcgtcc ggctcagagg gcgtggacct cgccaagacc
     2461 ggcgagttcg acgtgatcgt tctcgatctg atgctccccg acatgcacgg ctacgacgtc
     2521 tgccagcgct tgcgccttca gggcgatcac acgccgatcc tgatgctgac cgccatggac
     2581 gcgctggagg acaagatcaa gggcctgaag ctcggcgccg acgactatct caccaagccg
     2641 ttcgacttcg acgagctgct tgcccgaatt gaggccttgg tacgccggtc gcatagtttc
     2701 gcgccgagct cgaatgtcct taa
//
LOCUS       DASUQP010000177         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1315049, whole genome shotgun sequence.
ACCESSION   DASUQP010000177 DASUQP010000000
VERSION     DASUQP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315049"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1160..1348)
                     /locus_tag="VFY74_07825"
     CDS             complement(1160..1348)
                     /locus_tag="VFY74_07825"
                     /inference="COORDINATES: protein motif:HMM:NF026213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931640.1"
                     /translation="MTKARADFDKGEYRWVAEVMKRAAFANSDNAELPPMPMSSLAIR
                     PWRERWCHWQRRAPQNRL"
     gene            complement(1389..2138)
                     /locus_tag="VFY74_07830"
     CDS             complement(1389..2138)
                     /locus_tag="VFY74_07830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5931641.1"
                     /translation="MARRDRRRFAACVCTIRTTPQSRRRARSDRRRDCYASGSTFLKR
                     DFRDRLLNLRRIRSSDALLAVFLPFAVIYFSVLLSLWLGESRDQFGLAGGGGFVSLVV
                     LALILAPICEEVGWHGYGVDSLRAGAGMMKATLLFALLWCAWHAPLVLISGTYQHQLA
                     TMENKIFVANFFVSIFPVAIIANWFYYKNERSIALAVLMHSMLNAASVLINAGQVAKI
                     IATVLYAAIAVALVFGDRRLFAQGPRNFLQA"
     gene            complement(2299..2544)
                     /locus_tag="VFY74_07835"
     CDS             complement(2299..2544)
                     /locus_tag="VFY74_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931642.1"
                     /translation="MLKKSVMAAVLAVAVLSPAYAADLCTDEHMAQMDGMVAKMTDEA
                     KKKEAMAALDQSKAAMKAGDNAGCMKYMDEAHKAMGM"
     gene            complement(2645..2914)
                     /locus_tag="VFY74_07840"
     CDS             complement(2645..2914)
                     /locus_tag="VFY74_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931643.1"
                     /translation="MPLNEIPKYFNPETRRFIDAVLEQAWQELDAQAPVDAASTRRAM
                     ARRKLAGTIVALISIGETDPAKLKRFALNAAKAWDAGAGRKAESR"
     gene            3237..3611
                     /locus_tag="VFY74_07845"
     CDS             3237..3611
                     /locus_tag="VFY74_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931644.1"
                     /translation="MVLTAEQHLRIAILYEKAAADRLGVPPQQRAAFARKAKWFRMKA
                     RIRAKKEAASLLQAEQQAAAEFVRAESALAEASLDQGRLEETLLSEARLVELRGAAEA
                     VPAKPRYRSIAERLASVRGTAG"
     gene            complement(3831..4070)
                     /locus_tag="VFY74_07850"
     CDS             complement(3831..4070)
                     /locus_tag="VFY74_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931645.1"
                     /translation="MAEKVTSLKLTGFEIEVEDDPVTLLLQTDKGPFSVEMRRNQLEK
                     LVYALSFVREAEEPLRRRRERRQARRLLARRRLKT"
BASE COUNT          843 a         1360 c         1312 g          828 t
ORIGIN      
        1 gatcgcgagc acgatggcaa tgcctttcca tcgggtttct cgtggtgggc tcatggtgtc
       61 attattctcg gtaaaagccc cctgcaagga aggcggccag gctcgcggag cctgacggcc
      121 actttatcta tcggtccaac cgcggcaggg gcggcacctg cagcggatgg gacggcaggt
      181 ccaactccga acttccgcat tcctccatcc gatctgcctt tgaagcgggg gcggagatga
      241 aaatagcgcc gaacccccgc gtcgcgaaag agacgcgacc ggcgtcccct ttctgacccg
      301 ggcaggaacg accttaacct cacggcaaga atttgagcga tagcctggtg ctgcctaccg
      361 gggaatgggc tttgtcaatg gggtttggaa ggaagcggcc taggaaaccc ccctggccgc
      421 ttcttctttg gtgagggata gtgtcatgcg tattgctggc gttgttgcag cactcatcgg
      481 gtgtgccgta ctggcagcgt gcttccatca tcggcaaact gccgtcgaat atgcgccgcc
      541 gccactctcg acatcgccct acaaatagcg gccgggaatg cccgcgggct aattctcgtg
      601 agttgattgc gaaggcccct gcgcggcggc tacggccgtg ctgagggttg gtgcatgatc
      661 ctcggctaag acgacccgcc ggcgctggcg tgacgcgccg tggtgatcac ctgctgcgac
      721 gctgagacgt cagcctcacc agcggcaaaa tgcaccagcg aagatgataa cgagagaagc
      781 gtcagcagtc ccgcgaacgt ccacgctgct agtggaacac gagtctttac ggggacaccg
      841 cctattgtcc tagtcatggc aattttccct tcaggagccc cactccgtgc agaatcaact
      901 tcgatcaatg cgtttagttc cagaattccg attcagattc ggggaacgtc gcgttacctc
      961 gcgcattaac tcagaagccg agtccaattc gatgcaaaca tcacatacca aaatcgccca
     1021 ctcgattggg aaaggcgtta acgaacggtt agaaacgagc ggacgggaga agctgccgca
     1081 acggcttatt gatttgcttg cgaggctcca cgaagccgaa gaacgcgaca ggtcactttc
     1141 tgcccgtaaa gaatagcgtc tataaacggt tttggggtgc gcggcgctgc cagtgacacc
     1201 accgctcccg ccaaggcctg atagccaagc tgctcatagg catcggcgga agctccgcgt
     1261 tgtccgaatt ggcgaaggcg gcgcgcttca tcacttcggc aacccagcga tactcgcctt
     1321 tgtcgaagtc ggccctcgcc ttcgtcaata cggccgctcc gccgcctcat atggccgata
     1381 tagccgggtc aggcttgaag gaaattgcgc ggtccttggg caaagagccg gcgatcgcca
     1441 aagaccagcg ccacagcgat cgccgcatag agaacggtgg cgatgatttt ggcgacctgt
     1501 ccggcattga tcagcaccga tgccgcgttc agcatcgaat gcatgaggac cgcaagggcg
     1561 atagagcgct catttttgta atagaaccag ttggcgatga tcgcgacagg aaagatgctc
     1621 acgaagaaat tggcgacgaa aatcttgttc tccatcgtcg cgagctgatg ctgataggtg
     1681 ccggatatga gcacgagcgg cgcatgccac gcgcaccaca ggagcgcgaa caggagcgtc
     1741 gccttcatca tccccgcgcc tgccctcaga ctgtccacgc cgtagccgtg ccagccgact
     1801 tcttcgcaga tcggcgcgag gatcagcgcc agcacgacga gagagacgaa accccctccg
     1861 cccgcgagcc cgaactgatc gcgcgattct ccgagccaca acgacagaag aaccgaaaaa
     1921 tagatcaccg cgaacggcag gaagacggca agcagcgcgt cgcttgatct gatccgccgc
     1981 agattgagca gccggtccct gaaatcgcgc ttgagaaagg tactgcccga ggcgtagcaa
     2041 tcgcggcgcc gatcggaccg agcacgccgg cgagattgag gagttgtgcg tatggtgcaa
     2101 acgcaggccg cgaagcggcg gcgatcgcgc cgagccatga aacccaactc gcggcgtagg
     2161 caagtgcaaa gaacagcgcg ggccggtagc ggtagggttc cgtcacagcg ctcccgcgtg
     2221 cgatctccac gtccgatcga gcgcgggagg cgaccggtag aatcgcctcg cgcctcgatg
     2281 aattcggcag gccttgggct acatgcccat ggccttgtgc gcctcgtcca tgtacttcat
     2341 gcagcccgcg ttatcgcccg ccttcatcgc cgccttagac tgatcgagag ccgccatcgc
     2401 ctcttttttc ttggcctcgt cggtcatctt ggcgaccatg ccatccatct gcgccatatg
     2461 ctcgtcggtg cataggtccg cggcataggc aggggacaag acggcgacgg cgagtacggc
     2521 cgccatcacg gatttcttga gcatggcttt cctccttcat gttagaccgc ggtgcgcgga
     2581 catcagctaa acgcgcaata cggccatttg gctgccgacc ggaccgcgat ttttccgaac
     2641 ccgatcagcg gctttcggcc ttgcgccccg cccctgcgtc ccatgctttt gccgcattga
     2701 gagcgaaccg cttcagcttg gccgggtcag tttccccgat cgagatcagc gcgacgatcg
     2761 tgcctgccaa tttccgccgt gccatggcac ggcgcgtgga ggcggcatcg acgggggctt
     2821 gtgcgtcaag ctcctgccaa gcctgctcca gcaccgcatc gatgaaacgg cgcgtttccg
     2881 gattgaaata tttcggaatt tcgttgagag gcacggccca ctccccacgc aagagtgtcg
     2941 ctcaaaccag tgcgccccat cgagccgaag ctctcattga ctatacgcct tcccgcgcgc
     3001 gggcgcgagt cgccttgcgc cttgatcgtg ctcgtgcttt tgccgccgca gggcggcgcg
     3061 ccgtcaggca atgcctcgcc ggccgactcg gcaatgggga agagcggctc tcggcggtca
     3121 ttccttgtcc gggcgcggtc ctgcagcgga gggaccgcgg cgcgatattt gtcgctcgag
     3181 cggcacgctt tgggcgcgtt aaggtactct taaagcggag cggccatgct cgccccatgg
     3241 tcctcacggc ggaacagcat ctccggatcg cgattcttta tgagaaggcg gcggccgacc
     3301 ggctcggcgt tccgccacag cagcgcgccg cctttgcgcg gaaagcaaaa tggttccgca
     3361 tgaaggcgcg catcagggcc aagaaagagg cggcgtctct actccaggcc gagcagcagg
     3421 ccgcggcgga gttcgtgcgt gccgaaagcg ccctggccga ggcttccctc gaccaaggtc
     3481 gtctcgagga aactcttctg tcagaggcgc gactcgttga gctgcgcggc gcggccgagg
     3541 cggtgccggc aaagccgcgc taccgctcga tcgccgagcg tcttgcctcg gtgcggggca
     3601 ccgcaggctg aggcgctaga accgccggca aatcgctggt gcaaagcctc gatatattct
     3661 ctttgcgctg ctccgcgcaa ttctcctcgc cctgaggagg gccgaaggcc ccttttcgga
     3721 aggtcccctt gaaacacgtg cttcgagacg cccccttcgg ccctcctcag catgaggttt
     3781 tgcgggggaa ctagctccgg ccttatcgtc ggcttggttc tttaatcccg ttaggtcttc
     3841 aatcgtctgc gtgcgaggag ccgccgcgcc tgccgtctct cgcgccgcct gcgcaacggt
     3901 tcctccgctt cgcgaacaaa gctgagcgca tagacgagct tttcaagctg atttctcctc
     3961 atctccacgc tgaaggggcc cttgtcggtc tgcaacagca aggtcacggg atcgtcttcg
     4021 acctcgattt cgaagccggt cagcttcagg ctggtgactt tctcagccat ctccctgccc
     4081 tctctcaaga ggcgtctcgg aagactatga atcgtacacg gactaggtgc aacccctacc
     4141 cgcacaccta tgggcgagcc gatcgccggc ggcgaagaat cggtcggacg atcaccaggc
     4201 aagacgggac ggtgctgctg ctctggccta ttagggcggg gcgtcgcgct acgaagaaaa
     4261 atcgcccaag accgaaaaat ccgcaggagc tttggtgagc gcggcgtcac gtgtcttcgc
     4321 cggcgcgtcg tccggcgtct tcg
//
LOCUS       DASUQP010000178         4301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1315616, whole genome shotgun sequence.
ACCESSION   DASUQP010000178 DASUQP010000000
VERSION     DASUQP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315616"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="VFY74_07855"
     CDS             complement(<1..1513)
                     /locus_tag="VFY74_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX5931646.1"
                     /translation="MIINDLALLSLAVWAAYTLRLSRIYVPQDLDKWLVLAAAPLIGV
                     IVFYMRGLYKLVTRFIGPEGTTRIYIAVLIAAVLWAVAVLMSGVKDHPRSAIVIYALI
                     AALLIRLSRQWAGALLLAAAPQHKPASFDKHKNVIIYGAGTIGIQLLRALNEAGGYKT
                     VAFIDPNPSLAGQLVHGVKVVRPEKIGKVIADEKVKEVLLATPTALRGERRIALKVLE
                     AFPVVVKTLPALEEIASGHVQISDLRPIDVEDLLGRDPVTPDTDLLAANVRGKVVMIT
                     GAGGSIGSELTRQLLKLGPKTLVLLELSEVALYDISMEIENLKWRLQEEGEAGALASA
                     TAVIAVLGSVLDRKLVARTIQQHGVEVIYHAAAYKHVPIVETNPFAGLQNNTFGTLIV
                     AEVAKELGVERFVLVSSDKAVRPTNIMGASKRLAEQILQALAQEPGTGTNFTMVRFGN
                     VLDSSGSVVKLFRNQIKAGGPVTVTHPDVIRYFMSIPEAAQLVIQAGTMATGGE"
     gene            complement(1720..2403)
                     /locus_tag="VFY74_07860"
     CDS             complement(1720..2403)
                     /locus_tag="VFY74_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEX5931647.1"
                     /translation="MTVQDGARAKILERIRAALGAERLDRARRSAVARRLERHPSGTV
                     PSRAKRRGDERVALFADMLLKQGADVRRVATPKEAVGAIASYLGTCNLPGRLRMGADP
                     VLAALPWREAWDIERSFGAADPRDRASLSRAAAGAAETGTLFLLSGPDNPTTLNFLPE
                     CHTVLIGASDIFGSYEEAWDRIRALSGAGTLPRSVNLVSGPSRTADIEQTIVRGAHGP
                     RRLHVLILG"
     gene            complement(2400..3827)
                     /locus_tag="VFY74_07865"
     CDS             complement(2400..3827)
                     /locus_tag="VFY74_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX5931648.1"
                     /translation="MAETPQAFKQAAGRALADPALQKALLNVKRGFVTKRDMAKANLP
                     EFDLLREEARAIKDHTLAHLDLYLEAYERKVTESGGHVHYALDAAEARAIALRLCQEA
                     KAKRVAKGKSMVSEEIGLNAHLQASDIEVVETDLGEYVAQARGERPSHIIAPIIHLNK
                     ESVERDFRRKHRHLAPQRRLDQAEHLVAEARQVLRETFLTADAGITGANFLIAETGSA
                     VMVTNEGNGDLTATLPRLHIVVASIEKLVPTLEDASTLLRLLARSATGQDITTYTTFV
                     TGPRRDGDPDGPAAYHVVLLDNGRSEMLGTEFAAMLRCIRCGACLNHCPVYGAIGGHA
                     YGSVYPGPMGAVLTPQLQGIDRARDLPNASSFCGRCQAVCPLDIPLPDMMRAWRERDF
                     ARGNPPFKERLVLKLWAFAAKRPRLYHALTRAAARLLARRAGTRGRLGGLPLLKTWTG
                     SRDLPAPQGRTFHVLYARAQRGKTS"
     gene            complement(3835..>4301)
                     /locus_tag="VFY74_07870"
     CDS             complement(3835..>4301)
                     /locus_tag="VFY74_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5931649.1"
                     /translation="ERAAGLAAKSFELTSFLVDVLGLTAVDAAFAGTVTYHDSCSALR
                     ELHVQDAPRRLLATVEGLSLVELADREACCGFGGAFSVKYPDISAAIVDAKLRDIAGT
                     GAATVLSGDLGCLLQIAGRLAREHETIEIRHVAEVLAGMTDEPAIAAPRSGR"
BASE COUNT          767 a         1391 c         1443 g          700 t
ORIGIN      
        1 cttccccgcc ggtcgccatc gttcccgcct ggatcacgag ctgagccgcc tccggaatcg
       61 acatgaaata gcggatcacg tccggatggg tgacggtgac cggaccgccg gccttgatct
      121 ggttgcggaa cagcttgacc accgagccgg aggaatcgag cacgttgccg aaccgcacca
      181 tggtgaaatt cgtcccggtg ccgggctcct gcgcgagcgc ctgaaggatc tgctcggcca
      241 gccgcttgct cgcgcccatg atattggtcg ggcgcaccgc cttgtcgctc gacacgagaa
      301 cgaaacgctc gaccccgagc tccttggcga cttccgccac gatcagggtg ccgaaggtgt
      361 tgttctgaag tccggcgaaa gggttcgtct cgacgatggg aacgtgctta taggcggcgg
      421 cgtgatagat cacctcgacg ccgtgctgtt gaatcgtgcg ggccacgagc ttgcgatcga
      481 gcaccgatcc gagcacggca atgacggcgg tggcggaggc taaggctcca gcctctccct
      541 cctcttggag acgccatttg aggttctcga tttccatcga gatatcgtag agcgcgacct
      601 ccgacagctc gagcagcacg agagtcttcg gcccgagctt gagcagctgg cgcgtaagct
      661 ccgatccgat cgagccgccg gccccggtaa tcatcacaac cttcccccgt acgttggcgg
      721 caagaaggtc cgtatcgggc gtcacgggat cgcggccgag caaatcctcg acatcgatcg
      781 gcctcaagtc gctaatctgc acgtggccgg aagcgatctc ctcgagcgca ggaagcgttt
      841 tcaccaccac cggaaacgct tccagcacct tcaatgcaat ccggcgctcg ccgcgcagcg
      901 ccgtaggggt ggccagcagc acctccttca ctttctcgtc ggcgatgacc ttgccgatct
      961 tttcgggccg caccaccttg acgccgtgga cgagctggcc ggcaagcgag gggttgggat
     1021 cgatgaaggc gacggtcttg taaccgcccg cctcgttcag cgcccgcaac agctgaatgc
     1081 cgatggttcc cgcaccatag atgatgacgt tcttgtgttt gtcgaagctc gcaggcttgt
     1141 gctgcggcgc ggccgcgagc agaagggctc ccgcccactg ccggctcagc cggatcagaa
     1201 gcgccgcgat cagcgcatag atgacgatgg ccgaacgcgg gtggtccttg acgccgctca
     1261 tcagcaccgc cacggcccac aacaccgcgg cgatgagcac ggcgatatag atgcgggtcg
     1321 tgccctccgg accgataaag cgggtcacga gcttgtagag cccgcgcata taaaacacga
     1381 tgacgccgat gagcggcgct gccgccagga ccagccattt gtcgagatcc tgcggcacat
     1441 agatccggct gagcctgagc gtataggccg cccacaccgc gaggcttaag agcgcgagat
     1501 cgttgatgat caagacggcc cgcttgaacc agcgaggacg ctcgattagc catacggcaa
     1561 gacgcatgaa gggccacccc gaaccggtct tggaagccgg acgcgcctca cccgcacggc
     1621 gcgtgagagc gttgcacgat aaccgaagcc caatccgaac ggaagtgagc ctgcgctcca
     1681 ggattttcga ggaaaccgaa ggcactcagg ggtgggggct tagccgagga tcagcacatg
     1741 aaggcgccgc gggccgtgag cgccgcgcac gatcgtctgc tcgatatcgg ccgtgcgtga
     1801 cggcccgctg acgaggttga cgctgcgcgg cagcgtgccc gcaccggaaa gcgcccgtat
     1861 ccggtcccaa gcctcttcgt aggagccgaa gatgtcggaa gctccgatca gcacggtatg
     1921 acactcgggc aggaagttga gcgtggtcgg gttgtcgggg cccgagagga ggaacaaggt
     1981 cccggtctcc gccgcgcccg ccgccgctcg cgacagcgag gccctgtcgc gagggtccgc
     2041 agcgccgaaa gagcgctcga tgtcccacgc ctcgcgccaa ggcagcgcgg caaggactgg
     2101 atctgcgccc atgcgcaagc ggcccggcaa gttgcaggta ccgagatagg aggcaatggc
     2161 gccgaccgcc tccttcggcg tggcgacgcg gcgcacgtcg gcgccttgtt tcaggagcat
     2221 gtcggcgaag agcgccaccc gctcatcgcc tctccgcttg gcacgagagg gaacagtccc
     2281 gcttggatga cgctcgagcc ttcgcgcgac cgcgcttcgg cgcgccctgt cgaggcgctc
     2341 ggcgccaagc gcggcgcgga tgcgctccag gatcttcgcg cgtgctccgt cctgaaccgt
     2401 catgaggtct tcccgcgttg cgcccgggca tagaggacgt gaaaggtgcg gccttgcggg
     2461 gcgggaaggt cgcgcgaacc cgtccacgtc ttgagaagag ggaggccgcc tagcctgccg
     2521 cgggtcccgg cccggcgcgc caagagccgg gcggcggcgc gcgtcaacgc atggtagaga
     2581 cgcggccgct tggccgcgaa cgcccagagc ttgaggacaa gacgctcctt gaacgggggg
     2641 ttcccgcgcg caaaatcgcg ctcgcgccac gcccgcatca tgtcggggag cgggatgtcg
     2701 agcggacaga ccgcttggca ccgtccgcag aagctcgagg cgttggggag atcgcgggcg
     2761 cgatcgatgc cttgaagctg cggcgtcagc accgcgccca tcggaccggg atagaccgag
     2821 ccataagcat gcccgccgat ggcgccatag accgggcagt gattgaggca ggcgccgcag
     2881 cggatgcagc gcaacatggc ggcgaactcg gtgccgagca tctcgctccg tccgttgtcg
     2941 agcagcacga catggtaggc ggcggggccg tcgggatcgc cgtcccggcg cggaccggta
     3001 acgaacgtcg tataagtggt gatgtcctgg ccggtggccg agcgggcaag gagcctgagc
     3061 agcgtcgatg cgtcctcgag cgtcggcacc aacttctcga tcgaggcgac gacgatatgc
     3121 aggcgcggca gcgtcgcggt caggtctccg ttcccctcat tggtgaccat caccgcggag
     3181 ccggtttcgg cgatcaggaa attggcgccg gtgatgccgg catcggcggt caggaacgtc
     3241 tcgcgcagga cttgtcgcgc ctctgccacg agatgctcgg cctggtcgag cctgcgctgc
     3301 ggcgcaaggt gccgatgctt gcggcggaaa tcgcgttcga cgctctcttt gttgaggtga
     3361 atgatggggg cgatgatatg gctcgggcgc tcgccccgcg cttgcgcgac atattcgccg
     3421 agatcggtct cgacaacctc aatatctgaa gcctgcagat gggcgttgag cccgatctcc
     3481 tccgacacca ttgacttgcc tttggcgaca cgcttggctt tcgcctcttg gcagaggcga
     3541 agcgcgatgg cgcgagcctc ggcggcgtcg agcgcgtagt gcacgtgacc cccgctctcg
     3601 gtcaccttgc gctcgtaggc ttcgagatag agatcgagat gggcgagcgt gtgatccttg
     3661 atggcgcgcg cctcctcgcg cagcaggtcg aactctggca ggttcgcttt tgccatgtcg
     3721 cgcttggtca cgaagccgcg cttgacgttg agaagcgctt tctgcaacgc gggatcggcc
     3781 agcgcccggc cggcggcttg cttgaacgcc tgtggggtct cggccatgcc ggcctcagcg
     3841 ccccgaccgg ggcgcggcga tcgccggctc atccgtcatg ccggccagca cctcggcgac
     3901 atgacggatt tcaatcgtct catgctcccg cgcgagtctg ccggcgattt gcaggaggca
     3961 gccgagatca ccggagagca ccgtggccgc gccggtcccg gcgatgtcgc gcagcttggc
     4021 gtcaacgatt gcggccgaaa tgtccggata cttgaccgag aacgcgccac cgaaaccgca
     4081 gcacgcctcg cgatcggcaa gttccaccag cgagagaccc tcgaccgttg ccaagagccg
     4141 ccgcggcgcg tcctgcacat gaagctcgcg caaggcggag caggaatcgt gataggtcac
     4201 ggtcccggcg aaagccgcat cgaccgcggt gaggccgagg acatcgacca gaaacgaggt
     4261 gagctcgaaa ctcttggcgg cgaggcctgc ggcccgctcg g
//
LOCUS       DASUQP010000179        18081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1998559, whole genome shotgun sequence.
ACCESSION   DASUQP010000179 DASUQP010000000
VERSION     DASUQP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18081
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998559"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..322
                     /locus_tag="VFY74_07875"
                     /pseudo
     CDS             74..322
                     /locus_tag="VFY74_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(457..543)
                     /locus_tag="VFY74_07880"
     tRNA            complement(457..543)
                     /locus_tag="VFY74_07880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(507..509),aa:Leu,seq:cag)
     gene            700..1674
                     /locus_tag="VFY74_07885"
     CDS             700..1674
                     /locus_tag="VFY74_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX5931650.1"
                     /translation="MAITLDNKALVTVYGGSGFIGRHVVAAIAKTGARMRVAVRRPEL
                     AGHLQPLGGVGQINAVQANVRYPESLLAAARGADAVINLVGILFRTGKQTFEAVQDEG
                     ARHVAEAAWAEGAKAFVHVSAIGASERSRSVYARSKAAGEAAVKEVYPGAVILRPSVV
                     FGPEDDFFNRFAALARLLPALPLIGGGRTRFQPVFVGDVAKAVLAGLTGRADAGAPYE
                     LGGPELLTLKQVMERVLSYSMRDRWLVPVPFWFAKLQAAFLQFLPAPPLTIDQVRLLE
                     SDNVVSEDAKRGGRTLEGLGIEPVAIAAVVPGYLEQFRPRGQFSDYRP"
     gene            complement(1686..2483)
                     /locus_tag="VFY74_07890"
     CDS             complement(1686..2483)
                     /locus_tag="VFY74_07890"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001393.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5931651.1"
                     /translation="MTIEQIIVLAFVQGVTEFLPISSSGHLILVPKIFHWQDQGFVVD
                     VMTHLGTLFAILIYFWRDVAHIVRGALELLKGKITDEGRLAIYIVLATIPAIAFGIFL
                     KELGYTDLERNVAVVAWNTIFYGILMLVADMVGPQNRTMANMTLGSALLIGVAQALAL
                     IPGTSRSGVTMTAGRFLGFTRPDCARFSFLLGIPATAGAIVFTIGDAMASGEGITMDA
                     VYCAALTFVAGILAIAFLMNLLRRISFLPFVLYRMVLGGFLLVLLYT"
     gene            2585..3271
                     /locus_tag="VFY74_07895"
     CDS             2585..3271
                     /locus_tag="VFY74_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5931652.1"
                     /translation="MATLLHYPLCPFSRSIRLALAECGVEAELAEERPWEWRPEFVAL
                     NPAATLPVLIDDERGPIVGAYAISEYLGDTADERDPRLFQPFPGDEAAKAETRRLVDW
                     FHRKFHDEVTAYLVEEKVYRRFGPRSTSPDMEAMRAGHENLRYHLAYIGHLAETRSWL
                     AGDAMSFADLAAAAHLSALDYLGEVPWEEHEQAKNWYALLKSRPSFRPLLQDRLAGFT
                     PSGTYADLDF"
     gene            3296..4414
                     /gene="queG"
                     /locus_tag="VFY74_07900"
     CDS             3296..4414
                     /gene="queG"
                     /locus_tag="VFY74_07900"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX5931653.1"
                     /translation="MAETLKQRIAAEAEALGFDAVRFTSADAVEGAGASLDAFLAEGR
                     HGDMGWLATTSQRRKAPRALWPEAKSVILLGLNYGRAADPLAILNEKSRGAISIYAQG
                     DDYHEVLKAKLKPLAARVQDLTQSEVKIFVDTAPVMEKPLAARAGLGWQGKHTNLVSR
                     ELGSWLFVGSIFTTAEIAPDAPEEDHCGACRRCLDICPTQAFTAPYQIDARACISYLT
                     IEHKGHIAPRFRAAMGNRIFGCDDCLAVCPWNKFAQAAREAKLSVKAGSDNPPLAELL
                     ALDDATFRARFRGTPIKRTGRDRFLRNVLIAAGNSGDAALVPLVEARLHDDSPLVRAM
                     AVWALARLAPRRFNALRGMQGAAEPDQAVRSEWLEEAA"
     gene            4411..5283
                     /locus_tag="VFY74_07905"
     CDS             4411..5283
                     /locus_tag="VFY74_07905"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5931654.1"
                     /translation="MSRLFAFGLGFSALALARRLSAKGWQIAGTARDEGKIEGLEREG
                     YDAVRFAGEARNEALVDHLSGTTQLLHSIPPSAKGDPVLASYREAIAALPSLAWIGYL
                     STVGVYGDQEGGWVDESTEPKPNSARTDARVEAEQAWLRFGQETGVPVHVFRLAGIYG
                     PGRSVFDKLKEGTARRINKDGQVFSRIHVEDIASVLEASIARPRSGAIYNVADDEPAA
                     PGDVVAYAAELMGMPPPPEVAFAEADLSPMARSFYEGSRRIRNTRIKSELGVKLRYPT
                     YREGLAALLADHAQ"
     gene            5280..5472
                     /locus_tag="VFY74_07910"
                     /pseudo
     CDS             5280..5472
                     /locus_tag="VFY74_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            5472..5678
                     /locus_tag="VFY74_07915"
     CDS             5472..5678
                     /locus_tag="VFY74_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5931655.1"
                     /translation="MRYAVVIEKANGNDSAYVPDLPGCVVTGKTVAAVEEEIRAAIRF
                     HIDGLKAEGLEVPAPPSIADYVEA"
     gene            5743..5862
                     /locus_tag="VFY74_07920"
     CDS             5743..5862
                     /locus_tag="VFY74_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5931656.1"
                     /translation="MFRRSAARRLRIGDYWVLFSITPAQIEVIAVRHRKDAYI"
     gene            5875..6258
                     /locus_tag="VFY74_07925"
     CDS             5875..6258
                     /locus_tag="VFY74_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5931657.1"
                     /translation="MSKTQIINTEQGEELVVLTRREYDAMRARLGDEAAEDAMSVRIF
                     EERAGAVAIPVAVWEEIEAAPSPVGPLRKWRKLTQATLAAKAGISQPYLSAIESGRKT
                     GDVATLRAIATALGVGLDDVTREEG"
     gene            6348..6686
                     /locus_tag="VFY74_07930"
     CDS             6348..6686
                     /locus_tag="VFY74_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5931658.1"
                     /translation="MAIISTAVAVLGGGIAQGAGERAPCTEDAMIVFDASGSMAGNGW
                     GYGSETAGQVSRIDKVRASLAKILPSVTRHRRVGLITYGPGVWNRATCGSALRRKRTR
                     RRASWRRSTR"
     gene            6665..7042
                     /locus_tag="VFY74_07935"
     CDS             6665..7042
                     /locus_tag="VFY74_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931659.1"
                     /translation="MAAINALTPAGKTPLTAAVAQAADVLDFRAKPGLIVLLTDGEET
                     CGGSPCDLGKELSGEAAALTVHVVGLRVKGYTWMGEQSLLETKCLAERNGGLYLTVET
                     EDELQAAFEKTLGCPMVTELQRE"
     gene            complement(7305..8102)
                     /locus_tag="VFY74_07940"
     CDS             complement(7305..8102)
                     /locus_tag="VFY74_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5931660.1"
                     /translation="MGAGEPQFLDFGDGPRLRRLAYRLDEAGGGGGLGLIWLPGFLSD
                     MASTKALALAAWAEDQGLPMLSFDYSGHGLSTGDLKEGAIGDWLAEASAMLGLLLAKT
                     PRAIAIGSSMGGWIALLLARSLALEGAVSKLAGLVLIAPAWDMTEELIWNRMTDEAKT
                     ALARDGVFHEPSLYGDPYPITRRLIEEGRGHLIGAGPLDLDLPVRILQGMRDPDVPWA
                     HAIKLVDRLRSEDVELTLIKDGDHRLSREQDLRRLESTVASLVASSS"
     gene            8120..9334
                     /locus_tag="VFY74_07945"
     CDS             8120..9334
                     /locus_tag="VFY74_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5931661.1"
                     /translation="MPEVTILQVVPRLDAGGSEQAALEIAEALVGAGASALVATEGGR
                     MATAITDGGGEIIPLPVASKNPLTILANARRLARLIAERGVDLVHARSRAPAWSAFLA
                     ARRTRRPFVTTYHGAYGDPGPLKSAYNSVMGRGDRVIANSRYTADLIASRNPQARERI
                     RIIYRGVDLAKFAPGAVSEDRLARLRSAWGVNKTQPIVLQAARLTRWKGQVDVIEAAL
                     LLLAEDRVGDGVFILAGDAQGREGYRQELEQLIAGLSGRVKLVGHCADMPAAFALAQV
                     AVVASTEPETFGRTSIEAQAMGCPVIVTDIGAAPETVVPVSSDRQAFTGWVVPCRDPA
                     AIAKRIAGAVALSPEARAKLGARAQRHVAANFTLGQMQRATLSVYDELLGTSLAARFE
                     QRAKPFPASSSS"
     gene            9499..10035
                     /gene="infC"
                     /locus_tag="VFY74_07950"
     CDS             9499..10035
                     /gene="infC"
                     /locus_tag="VFY74_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008037964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5931662.1"
                     /translation="MVRRPTKLTPDRGGPRINEGIETPNIQLIDAAGHNVGVVAVTEG
                     LARAAEAGLDLVEIAPNSEPPVCKILDYGKYKYQAQKKAAEARKKQRTVEIKEIKMRP
                     NIDTHDYDVKMRSIRRFFEEGDKVKVTLRFRGREMAHQELGAQLLDRVKQDTSTIAKV
                     ESEPRLEGRQMVMVLAPR"
     gene            10203..10406
                     /gene="rpmI"
                     /locus_tag="VFY74_07955"
     CDS             10203..10406
                     /gene="rpmI"
                     /locus_tag="VFY74_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5931663.1"
                     /translation="MPKLKTKSGAKKRFKLTATGKVKCQASRKRHGMIQRPTKFIRTA
                     RGTMVLSDEDAKIVKKHFLPYSR"
     gene            10437..10799
                     /gene="rplT"
                     /locus_tag="VFY74_07960"
     CDS             10437..10799
                     /gene="rplT"
                     /locus_tag="VFY74_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5931664.1"
                     /translation="MSRVKRGVTSHARHRKVLKQAKGYYGRRKNTIRVAKQAVEKAGQ
                     YAYRDRKVKKRTFRALWIQRINAAARANGLTYGRLIDGLKKAGIELDRKVLADIAVRE
                     PATFKSLVEAASAAAPRA"
     gene            10843..11928
                     /gene="pheS"
                     /locus_tag="VFY74_07965"
     CDS             10843..11928
                     /gene="pheS"
                     /locus_tag="VFY74_07965"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5931665.1"
                     /translation="MSALELYSLKTELLHAIAEASDLDKLEEVRIAALGKKGRISELM
                     QALGSLPPDERKAYGQVVNSLKTAVGEALEARRQELGRSATAERLVHERADVTLPVRE
                     GPLAEGRIHPVSQVTEEITEIFADMGFSIAEGPDIETDFNNFTALNMPAEHPARQDHD
                     TFYFPPAPDGTRALLRTHTSPVQIRAMLKTKPPLRIIAPGRVYRSDSDQTHTPMFHQV
                     EGLVIDEETHLGHLKWVLEEFCKAFFEIPTVKMRFRASHFPFTEPSMEVDIGYARQGD
                     AIRIGEGDEWLEILGCGMVHPNVLKNVGLDPQRYQGFAFGLGIDRIAMLKYGMPDLRA
                     FFAGDLRWLKHYGFPPLAIPTIAAGLS"
     gene            11932..14343
                     /gene="pheT"
                     /locus_tag="VFY74_07970"
     CDS             11932..14343
                     /gene="pheT"
                     /locus_tag="VFY74_07970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5931666.1"
                     /translation="MKFTLSWLKEHLDTKASVAAIAETLTRIGLEVEEVFDPAAALKP
                     FRVAEVVRCEKHPNADKLSVCEVDTGAERVQVVCGAPNARAGMKGVFAAVGAYVPGIG
                     LTLTRAKIRGVESSGMLLSERELELSDEHTGIIELSRDAKVGSPAAKALGLDDAVFHI
                     AITPNRPDCLGVRGVTRDLAAAGLGTLKKDKVKPVKGGFANPVPIALEFDKASAGACP
                     VFAGRIVRGVKNGPSPDWLQRRLKAIGLRPINALVDITNYVSYDRGRPLHVYDADKLN
                     GTIRARMGRLSEKFLALDGKVYEVDEDMCVIADDGAVLGLGGVIGGEDTAVTEATTNV
                     FIESAYFDPKRTARTGRKLGIQSDARFRFERGVDPEFVVPGLELATDLVLRLCGGAAS
                     KIALAGRPPKPNKPFKFDPALVERLSGLDLARGEIKRLLDALGIALDGKGKHLKAAPP
                     SWRPDITGPADLVEEVVRLKGIDAVPATPMRRDAGLAKPVLTDAQRRQRLTRRVLAAR
                     GLVETVTWSFIPPEHAALFGGGAPELTLSNPISTELAAMRPSLLPALIAAAQRNRDRG
                     FADGALFEIGQAYRGPKPDDQFVTAAGVRFGRSSLAGSGRHWSGEAPPADVFAAKADA
                     VAVLAALGLDQANLAGTREAPAWFHPGRSGALMLGPKTVLGVFGEFHPDLLAKLGIDP
                     PIAGFELFLDAIPASRRRSASQALEASDLQPVRRDLAFVIDGEIAAGDVVRAALSADR
                     ALISDVGVFDVFTGRGVPEGKKSLAIEVTLQPRDKTLTDAEIDAVAEKVVAAVKKATG
                     GELRG"
     gene            complement(14638..16074)
                     /locus_tag="VFY74_07975"
     CDS             complement(14638..16074)
                     /locus_tag="VFY74_07975"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX5931667.1"
                     /translation="MHGRLKSRVMVAAGAVLLAFAASIPSAATRPGPCDRPELPAAIG
                     AGEPAQRASPDRLTIVLAGDTGFNTTDAKVDAKGMQKGKQVTSFADSLAGVASAVDGD
                     LAFVNLETVVTDRNDLAPEGRGRGTFHFRSHPDALKALIQSGFNLFSLANNHAYDYGA
                     QGIEETLYHLAVANAERAIAFAGTGSNFEEAIRPGCLDVGGARIGFAAIGILTGDQPQ
                     SRAGPNKAGQASYRDRPDFVAVVDKLASMPADYRILSLHYGVEGRVVPDERQLDDWRK
                     FAAEEKGIDLIVGHHPHVAQGVELNGKSLIFYGLGNFLHPGTLEPARFGLCRDYGLMS
                     KVHLARSGGAWRVEAIEAIPLTNTQSRPERFVAGEGTKRIHALNHLAAGLDDGKNARG
                     VRFAPRPDGSGLYCADGAAARGGEIGTLCQGWQPPADPDKALAATIASACEDKPFYGK
                     PALVKRRVPQRRRAGPSSPSPFGTVGPQ"
     gene            complement(16085..17746)
                     /locus_tag="VFY74_07980"
     CDS             complement(16085..17746)
                     /locus_tag="VFY74_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX5931668.1"
                     /translation="MQERLSSPQSAISRAYDVVVVGSGYGGGVAASRLARAGQSVCVI
                     EKGKEFLTGEFPSRLPEIRRELQLNGAKLRSGSRTGLFDFRLGADIHVLVGCGLGGGS
                     LINAGVALRPDPRVFADSAWPEAVSGDGLLDLGFARASAMLRPSRYRNAPSLTKYRAL
                     EAASAAFDVPPVAAPVVVSFEDVVNPAGVAQPACTLCGDCCSGCNVGAKNTVAMTYLP
                     DAKAHGAAIFTELSVTHIAKEEGGWRVHFVASDDREASPAFVDARTVVLAAGTLGSTE
                     ILLRSREYGLSLSDRLGEKFSANGDIIAFALGGSERVNAVGVGHPPKFLGDAIGACVA
                     GQIELADGEVLDRSMILQEGVMPSALAPLLPVFFIAGGRLLGAAQSLIKGVYHGPLSH
                     LHTFFVVSHDEAAGRITLHNGGAEVQWPGVADQPVYARVDDALTKAAKAVGARYIKSP
                     LAATSVGTKPATAHPLGGCGMGEDAGSGVVNHKCQVFDGRARDSVHAGLYVCDGAVIP
                     RSIGVNPLLTISALAERAMVHLARDHNLGFDDGARIRQELDQALS"
     gene            17900..>18081
                     /locus_tag="VFY74_07985"
     CDS             17900..>18081
                     /locus_tag="VFY74_07985"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEX5931669.1"
                     /translation="MIAKSVCTALAAALLAALVLAFGGYEARAEATKTAVFAGGCFWC
                     VEDAFDHVDGVTETVSG"
BASE COUNT         3202 a         5968 c         5966 g         2945 t
ORIGIN      
        1 ccagcatgtg tcaggttcca tccagaccga gaagagcggc tacaaggtcg aggttccggt
       61 catcatcctg ccggtgcttc aaaagaccat tgatgccggg ccttgtggcg atctcacctt
      121 catcgtgggc gagaatggcc gaccgctcac gaaggagacc ttcggcaatg tgttcaaggc
      181 cgcctgcaag gccgctggcg ttccaggctc agcgcacggt gtccgcaaga tcgcggcgac
      241 cacggcggcc aatgctggcg cgacggaagc ggagttgatg gcgatattcg gatggactga
      301 tccgaagatg gcggcgcact acacgcgcac cgccaacagg aagcggcttg ccgcccaggc
      361 gatgtccaaa ttggcaacat ctattcccgc accttgtcct cagggtgcgg gcgcgagcgg
      421 taaaagccca atgaaaacag agctatattt tgccgatggt gcccaggaga agactcgaac
      481 ttccacgccc tttcgggcac tagaacctga atctagcgcg tctaccagtt ccgccacctg
      541 ggcttgaggc cccttgaggt acgatcggga cttgcgaaag tcaatcagcc ctgccccccg
      601 cgactctaag atgtcgacgt cacgagagcg gcggcgcttc acagggttcg gccacctcgt
      661 gtaaagctcg catcaaggag cggggtgcga ggacagcgcg tggccatcac tctcgacaac
      721 aaggcgcttg tgaccgtcta cgggggctcg ggcttcatcg gccgccatgt ggtcgccgcc
      781 atcgccaaga ccggcgcgcg catgcgcgtt gccgtgagac ggcccgagct cgccggccac
      841 ctccagccgc ttggcggtgt cggccagatc aacgccgtgc aggccaatgt gcgctatccg
      901 gagtcgcttc ttgccgcggc gcgcggcgca gacgcggtca tcaaccttgt cggcatcctg
      961 ttccggaccg gcaagcagac cttcgaagcg gtccaggacg agggtgcccg gcatgtggcg
     1021 gaggctgcat gggcggaagg cgccaaggcg ttcgttcatg tctcggcgat cggcgcgagc
     1081 gaacggtcgc gctcggttta cgcgcggagc aaggccgcag gcgaggccgc ggtcaaagag
     1141 gtctatcccg gcgccgtcat cctcaggccg tcggtcgtgt tcgggcccga agacgatttc
     1201 ttcaaccgtt tcgccgctct cgcgagactc ttgccggcgc tccccctcat cggcggcggc
     1261 cggacgaggt ttcaacccgt gttcgtgggc gatgtggcta aggccgttct tgccgggctt
     1321 accggcaggg cggatgcggg tgcgccttac gagctcgggg gaccggagct gctcacgctg
     1381 aagcaagtga tggaacgcgt gctgtcctat tcgatgcgcg accgctggct cgtgcccgtg
     1441 ccgttctggt tcgccaagct ccaggcagct ttcctgcaat tccttcccgc tccaccgctg
     1501 accatcgatc aagtgcggct gctcgagagc gacaacgtcg tgagcgaaga cgcaaagagg
     1561 ggcggccgca cgctcgaagg cctcggcatc gagccggtgg caatcgccgc ggtcgtgccc
     1621 ggctatctcg agcaattccg tccgcgcggg cagttctccg actaccgccc ctgaggcttg
     1681 aaccctcagg tgtagagaag caccaacaga aagccgccca gcaccatgcg atagagcacg
     1741 aagggcagga agctgatgcg gcggagcaga ttcatcaaga acgcgatggc gagaatgccg
     1801 gcgacgaaag tcagcgccgc gcagtagacc gcatccatgg tgatcccctc gccgctcgcc
     1861 atcgcatcgc cgatggtgaa gacgatcgct cccgccgtgg ccgggatacc gagcaggaag
     1921 gagaagcggg cgcaatccgg gcgcgtgaag ccaagaaagc gccccgcggt catcgtcacc
     1981 ccggagcggc tcgtgccggg aatgagcgcc aaggcctgcg ccacgccgat gaggagcgcg
     2041 ctgccgagcg tcatgttcgc catggtacgg ttctgcgggc cgaccatatc ggccaccagc
     2101 atcaagatgc cgtagaagat cgtgttccag gccacgaccg cgacattccg ttcgagatcg
     2161 gtgtagccga gctccttcag gaaaatgccg aaggcgatgg cggggatggt ggccagaacg
     2221 atgtagatgg caagccggcc ctcatcggtg atcttgccct tgagcaattc gagtgcaccc
     2281 cgcacgatgt gcgccacgtc gcgccagaaa tagatgagga tggcgaacag cgtgccgagg
     2341 tgggtcatga catcgaccac gaacccctgg tcctgccaat gaaagatctt gggaaccaga
     2401 atgaggtgcc ccgaggagga aatgggcagg aactcggtca ccccctggac aaaggcaagg
     2461 acgatgatct gctctatcgt catgaggtcc cccgctcccc gcttgttccc tgcgcgtgca
     2521 ggttctatac gaggctcaag tctcgcgcga ttcattttgc aacgactggc cgccgcacgg
     2581 gtcgatggcg acgcttctgc actatccgct ctgcccgttc tcccgatcga tccggctcgc
     2641 gctggccgag tgcggggtgg aggccgagct cgccgaggaa cggccgtggg agtggcggcc
     2701 ggagttcgtg gcgctgaacc ctgccgcaac gctgccggtg ctcatcgacg acgagcgcgg
     2761 gccgatcgtg ggcgcttacg ccatctcgga atatctgggc gacaccgccg atgagcgcga
     2821 cccccgcctg ttccaaccgt tcccgggcga tgaagcggcg aaggcggaga cgcggcgcct
     2881 ggtcgactgg ttccaccgca agtttcacga cgaggtcacc gcgtatctcg tcgaggagaa
     2941 agtgtaccgg cggttcggcc cgcgcagtac ctcgccggac atggaggcga tgcgggcagg
     3001 ccacgagaat ctgcgctatc atctcgccta tatcggccat ctcgccgaga cccggagttg
     3061 gcttgccggc gacgcgatga gcttcgccga tcttgccgcg gccgcgcatc tctcggcgct
     3121 cgactatctc ggcgaggtgc cgtgggagga gcatgagcag gccaagaatt ggtatgcgct
     3181 gttgaagtcg agaccgtcgt tccggccgct gctccaggac cgcctcgcgg gcttcacccc
     3241 ctccggcacc tacgcggatc tcgatttttg agtcatcagc tccgacatgt tgggcatggc
     3301 agagacgctg aaacaacgga tcgcggccga ggccgaggcc ctcggcttcg atgccgtgcg
     3361 cttcacgtca gccgatgccg tggagggcgc gggcgcctcg cttgatgcgt tcctggcgga
     3421 gggacggcac ggcgacatgg gctggcttgc cacgacctcg cagcgccgca aggcgccgcg
     3481 cgcgctgtgg cccgaggcga agagcgtcat cttgctcgga ctgaattacg gacgcgccgc
     3541 cgatccgctc gccattctga acgagaaatc gcgcggcgcg atctcgatct acgcgcaagg
     3601 cgacgattac cacgaggtcc tcaaggcgaa gctgaaaccg ctcgccgcac gcgtgcaaga
     3661 tctcacgcaa agcgaggtga agatcttcgt cgataccgcc cccgtgatgg agaagccgct
     3721 cgccgcccgc gccggtttgg gctggcaagg caagcacacc aatctcgtct cgcgcgagct
     3781 tgggtcatgg ctcttcgtcg gctcgatctt caccacggcc gagatcgctc ccgacgcgcc
     3841 ggaagaggat cattgcgggg cgtgccggcg ctgcctcgac atctgtccca cgcaggcatt
     3901 caccgcgccc taccaaatcg atgcgcgcgc ctgcatctcc tatctcacca tcgagcataa
     3961 ggggcatatc gcgccacgct tccgcgcggc gatgggcaat cgcatcttcg gctgcgatga
     4021 ctgtctcgcc gtctgcccct ggaacaagtt cgcgcaagcc gcgcgcgagg cgaagctcag
     4081 cgtaaaagcg ggaagcgaca acccgccgct cgcagagctt ctcgctctcg acgatgcaac
     4141 ctttcgcgcc cgcttccgcg gcacgccgat caagcgcaca gggcgcgacc gattcctgcg
     4201 caacgttctc atcgccgccg gcaattccgg cgacgcggcg ctcgtgcctc tggtcgaggc
     4261 gcggcttcac gacgattcgc ccttggtgcg cgccatggcc gtgtgggcgc tggcgcgcct
     4321 tgcgccccgg cgcttcaacg ccttgcgcgg catgcagggc gcagccgagc ccgatcaggc
     4381 ggtgagaagc gaatggctgg aggaagcggc atgagccggc tgtttgcctt cggcctcggt
     4441 ttctcggctc tggctctggc gaggcgcctt tcggccaaag ggtggcagat cgcgggcacc
     4501 gcgcgcgacg aaggcaagat cgaagggctg gagcgcgagg gctacgatgc cgtccgcttt
     4561 gccggcgaag cgcgcaacga ggcgctggtg gaccatcttt ccggcacgac ccagctcctc
     4621 cattccattc cgccgagcgc caaaggcgat cccgtattgg catcgtatcg cgaggcgatc
     4681 gccgcgctcc cttcgctcgc atggatcggc tatctctcga ctgtcggcgt ctatggcgac
     4741 caggaaggcg gctgggtcga cgaaagcacg gagcccaagc cgaacagtgc acgtacggac
     4801 gcccgcgtcg aagccgagca ggcctggctc cggttcgggc aggagaccgg cgttccggtg
     4861 cacgtgttcc ggctcgcggg catctacggg cccgggcgga gcgtcttcga caagcttaag
     4921 gaaggcacgg cgcgccggat caacaaggac ggccaagtgt tcagccgcat ccatgtcgag
     4981 gacatcgcaa gcgtgctaga agcctcgatc gcccggccgc gcagcggggc gatctacaat
     5041 gtcgccgacg acgagccggc ggcgcccggt gacgtggtgg cctatgcggc ggagctgatg
     5101 ggcatgccgc cgccgcctga ggtcgcgttt gccgaagccg atctttcgcc catggcgcgg
     5161 agcttctatg agggcagccg gcgcattcgc aacacgcgca tcaagtcgga actcggcgtc
     5221 aagctgcgtt atcccaccta ccgcgaaggc ctcgccgccc tgcttgcaga ccatgcgcaa
     5281 tgaaggtccg cgaagtcatt cggattatcg aagccgacgg tggctcttgt cgccacgcgc
     5341 ggaagtcacc gtcaatatcg acatccgagc aaatctggtc gtgttacaat agccggcaag
     5401 ccctcggagg acttggcgcc cggcacgctc aacagcatct tcaagcaggc tggcctcaag
     5461 gagagacggt gatgcgatac gcggtcgtga tcgagaaggc taacggaaac gactcggcat
     5521 acgtgccgga ccttcccggc tgcgtggtaa cgggcaagac ggtcgccgcg gtggaagagg
     5581 aaatccgcgc tgcgattcgc ttccatatcg atgggctgaa ggctgagggt cttgaggttc
     5641 ctgctccacc gagcatcgcg gactacgtcg aggcgtgaat ctccggcgag aatgctgtca
     5701 ttctccttcc gacttgccgg cgctgtgtgg cactcatgac aaatgttcag gcggtccgcg
     5761 gcgcggaggc tcaggatcgg ggattactgg gtgctgttca gcatcactcc ggcccagatc
     5821 gaagtcatcg ccgtgcgcca ccgcaaggac gcctacatct agaaggccgc tatcatgagc
     5881 aagacccaga tcatcaacac cgagcagggc gaggagctcg tcgtgctcac ccgccgtgag
     5941 tacgacgcca tgcgcgcccg cctcggcgac gaggccgcgg aggacgcgat gagcgttcgc
     6001 atcttcgagg agcgcgccgg cgccgtggcc attccggtcg cggtgtggga agagatcgag
     6061 gccgcgcctt cgccggtcgg cccgttgcgc aagtggcgca agctgacgca agcgacgctc
     6121 gccgccaagg cgggcatcag ccagccctat ctttccgcca tcgagtccgg ccgcaagacc
     6181 ggagacgtcg ccacgctgcg cgccatcgcc accgcgctcg gcgtcggcct cgacgacgtg
     6241 accagggaag aaggttaggc gatcggccgg cgcgactagt cgtacgcaag ttcgagttca
     6301 aacgattgac ctctaacacg gcatcttcgc tccatggctt gcgcgccgtg gcgattatct
     6361 cgaccgcggt tgcggtactc ggcggcggta tcgcccaagg cgccggggag cgtgcgccct
     6421 gcaccgagga cgccatgatc gtgttcgacg cctcgggctc gatggcgggc aatggctggg
     6481 gctatggcag cgaaaccgcg ggccaagtga gccgcatcga caaggtgcgc gcctcgcttg
     6541 cgaagattct cccaagcgtc acgcggcatc gccgcgtcgg tctcatcacc tacggtcccg
     6601 gcgtctggaa ccgggcaacg tgcggctcgg ccttgcgccg gaagcgaact cggcgacgcg
     6661 catcatggcg gcgatcaacg cgctgacgcc cgcgggcaag accccgctga cggcggccgt
     6721 ggcgcaagcc gccgacgtgc tcgatttccg cgccaagccc ggcctcatcg tgctcctcac
     6781 cgacggcgag gagacgtgcg ggggctcgcc ttgcgatctc ggcaaggaac tgagcggcga
     6841 ggctgcggcg ctcacggtcc atgtcgtcgg tcttcgcgtg aaaggctaca cctggatggg
     6901 ggagcagagc ctcctggaaa cgaaatgcct cgccgagcgg aacggcggtc tctatctcac
     6961 cgtcgagacg gaggacgagt tgcaagcggc cttcgagaag acgctcggct gcccgatggt
     7021 gacggaactt cagcgggagt gagcgcactt ttcttcgtca gtccggcgca agcatacagc
     7081 aaattcccgc tctcgcctcg gggagagcac gcgcgccact tggcgcgcgg tgagggggca
     7141 ggcgcgaagc gccgctagat agattcaccc tcacccttcc ccccactcgc cgcgctttgc
     7201 gcgtcgggcg ggaagccctc tccctaggga gaggggaagc tcatggcgag cctgaagcat
     7261 gacgattaca ctctgcgtcc ttccagggcc gctgcgcggc cacctcagga tgacgaagcc
     7321 acgagcgaag cgacggtcga ttcgaggcgc cgcaaatcct gctcccgcga caggcggtgg
     7381 tcgccgtcct tgatgagggt gagttcgaca tcctccgagc ggagtcggtc cacaagcttg
     7441 attgcatggg cccacggcac gtcggggtcg cgcatgcctt gcaggatgcg caccggcaga
     7501 tcgagatcga gcggaccggc cccaatcagg tgcccccgcc cctcctcgat caagcgcctg
     7561 gtgatgggat agggatcgcc gtagagggag ggctcatgga agacgccatc gcgcgcgagc
     7621 gcggtctttg cctcgtcggt catccgattc catataagct cctcggtcat gtcccaggcg
     7681 ggcgcgatca gcacgagacc ggcaagcttg ctcaccgccc cttccagcgc gagcgaacgc
     7741 gcaagaagaa gcgcaatcca tccgcccatc gacgagccga tcgcgatcgc ccgcggcgtc
     7801 ttggcgagga gaagcccgag catggcggaa gcctccgcaa gccaatctcc gatcgcgccc
     7861 tctttgaggt cgcctgtgga cagcccgtgg cccgaatagt cgaagctgag catcggcaag
     7921 ccctggtcct cggcccaggc cgcgagcgcg agcgccttgg tgctcgccat gtcggagaga
     7981 aagcctggaa gccagatgag cccaagcccc ccgccgcctc ccgcctcgtc caagcgatag
     8041 gcgaggcggc gcaatcgcgg cccgtcgccg aagtcgagga attgcggttc gcctgcgccc
     8101 atcctaagaa ccttgttcca tgccggaggt gaccatattg caggtcgttc cgcgcctcga
     8161 tgccggaggc agcgaacagg cggcgctcga gatcgcggaa gcgctcgtag gcgccggcgc
     8221 gtccgccctc gttgccaccg aaggaggccg gatggcaacc gccatcaccg acgggggcgg
     8281 cgagatcatc ccgctccccg ttgcgagcaa gaacccgctc accatccttg ccaatgcgcg
     8341 gaggctcgcg cgcctcatcg cggagcgcgg cgtcgacctc gtccacgcga gaagcagggc
     8401 tccggcctgg agcgcattcc ttgccgcccg ccgcacgagg cgtcccttcg tcaccaccta
     8461 tcacggcgct tatggagatc cgggcccgct caagagcgcc tataacagcg tcatgggccg
     8521 cggcgaccgc gtcatcgcca actcccgcta cacggcagac ctcatcgcct cgcgcaatcc
     8581 acaagcgcgg gagcgcatcc gcatcatcta tcgcggtgtc gacctcgcca aatttgcgcc
     8641 cggagccgtc agcgaggatc gacttgcccg attgaggtcg gcctggggcg tgaacaagac
     8701 gcagcccatc gtcctgcaag ccgcgcgact gacgagatgg aagggccagg tcgatgtcat
     8761 cgaggcggcg ctcctcttgc tcgccgagga tcgggtcggc gacggcgtct tcatcttggc
     8821 cggcgacgct caagggaggg agggttatcg ccaagagctc gagcagctca ttgcagggct
     8881 ttccggccgc gtgaaactgg tcggccattg cgccgacatg cctgccgcct tcgcgcttgc
     8941 acaggtcgcg gtcgtggcct cgacggaacc ggaaaccttc gggcggacga gcatcgaggc
     9001 tcaggccatg gggtgccccg ttatcgtgac cgacatcggc gccgcgccgg aaacggtggt
     9061 gcccgtctca tcggatcggc aagccttcac cggctgggtg gttccctgtc gcgatcctgc
     9121 ggcaattgcc aaacgcatcg cgggggccgt ggcgctttcg ccggaagcgc gggccaaact
     9181 cggtgcgcgc gcgcagcgtc atgtcgccgc caatttcacc ttaggccaaa tgcagcgcgc
     9241 aacgctctcg gtctatgacg aactgctcgg cacgtcgctt gccgcccgtt tcgagcaacg
     9301 ggcgaagccg ttcccggcgt caagttcttc ttaaggaaaa gaatcttcgc gcaggcttgc
     9361 agggggcggg tccggcttga cttgtcaggc gcaatttcgt tgctatagaa gacgcatggc
     9421 gtgtcaactc ggactgcggc gcaaaaccgc ctttttgaag gggtctgccg ccgccgttcc
     9481 caaagaggag agcaaaacat cgtaaggcga ccgacgaagc ttacgcccga tcgggggggg
     9541 ccccgcatca acgagggcat tgaaactcca aacatccagc tcatcgacgc cgccggccat
     9601 aatgtcggcg tggttgccgt taccgaaggc ctcgcgcgcg cggccgaggc cggtctcgac
     9661 ctcgtcgaga ttgcgcccaa ctccgagccc ccggtgtgca agatcctcga ttacggcaag
     9721 tacaaatacc aggcgcagaa aaaggcagcc gaggcgcgca agaagcagcg caccgtcgag
     9781 atcaaggaga tcaagatgcg gccgaacatc gatacccacg actacgacgt gaagatgcgc
     9841 tcgatccgcc gcttcttcga ggagggcgac aaggtcaagg tcactttgcg cttcaggggc
     9901 cgcgagatgg cgcatcagga gctcggcgcg cagctgctcg accgggtgaa gcaggacacc
     9961 tcgaccatcg ccaaggtgga atcggagccg cggctcgaag gccggcagat ggtgatggtg
    10021 ctcgcgcccc gctagcggca tctaccgccg gaatcccttg catttgcgcc tggaccgacc
    10081 ctataaggtc gcgcgaagga gccgcccggc ggggcatgcc gtggcggctc gcatgctttc
    10141 caatcgtcga aacgcgcccc tcgctccccg ctatggaccg gggccaaacc caaggaagcc
    10201 aaatgcccaa gctcaagacc aagagcggtg ccaagaagcg cttcaaactc acggcgaccg
    10261 gcaaggtcaa atgccaggcc tcgcgcaagc gtcacggcat gatccagcgc cccaccaagt
    10321 tcatccggac cgcgcgcggg acgatggtgc tgtctgacga agacgccaag atcgtgaaga
    10381 agcatttcct gccctatagc cgctaggctc ggggcaaaag tcgaaggagc accctcatgt
    10441 cccgtgtgaa acgcggcgtc acctcccatg cccgccaccg caaggtgctc aagcaggcca
    10501 agggctatta cggccgccgc aagaatacga tccgcgtcgc caagcaggcg gtcgagaagg
    10561 ccgggcaata cgcctatcgc gaccgcaagg tgaagaagcg caccttccgc gccttgtgga
    10621 tccagcgcat caatgccgcc gcgcgcgcca acggtctgac ctatgggcgg ctgatcgacg
    10681 gcctgaaaaa ggccggcatc gaactcgatc gcaaagtgct tgccgacatt gccgtgcgcg
    10741 agccggcgac tttcaagagc ctggtcgagg ctgcgtctgc tgctgcgccg cgggcctgat
    10801 cccgcgaagc gggcctggcc tcgcgcttta ggagcctgag ccatgagcgc gcttgagctc
    10861 tacagtctca agaccgagct gcttcacgcc atcgctgaag cttccgatct cgacaagctc
    10921 gaagaggtcc gcatcgccgc gctcggcaag aagggccgca tctccgagct gatgcaggcg
    10981 ctcggctcgc tcccgccgga cgagcgcaag gcctacggcc aggtggtcaa cagcctgaag
    11041 acagcggtcg gcgaggcgct cgaagcgcgg cggcaggaac ttgggcgcag tgcgaccgca
    11101 gagagattgg tgcatgagcg cgccgacgtc acgctccccg tgcgcgaagg gccgctcgcc
    11161 gaggggcgca tccatcccgt cagccaggtg accgaggaga tcaccgagat cttcgccgat
    11221 atgggtttct cgatcgccga gggtcccgac atcgagaccg acttcaacaa tttcaccgcg
    11281 ctcaacatgc cggccgagca tccggcgcgg caggaccacg acacattcta tttcccgccc
    11341 gcgcccgacg gcacgcgcgc gcttctgcgc acccatacga gcccggtgca gatcagagcc
    11401 atgctcaaga ccaagccgcc attgcgcatc atcgcgccgg gacgcgtcta ccgcagcgat
    11461 tccgaccaga cccacacgcc gatgttccac caggtcgagg ggctggtgat cgacgaggaa
    11521 acccatctcg gccacctgaa atgggtgctc gaggagttct gcaaggcctt cttcgagatt
    11581 cccacggtca agatgcgctt tcgcgcgagc catttcccct tcaccgagcc gtcgatggag
    11641 gtcgatatcg gctacgcgcg gcagggcgac gcgatcagga tcggcgaagg cgacgagtgg
    11701 ctcgagatcc tgggctgcgg catggtgcat ccaaacgtgc tcaagaatgt cgggcttgac
    11761 cctcagcgct atcagggctt cgccttcggg ctcggcatcg accgcatcgc catgctgaaa
    11821 tacggcatgc cggatctgcg cgccttcttc gccggcgatc tccgttggct caagcactac
    11881 ggcttcccgc cgctcgccat ccccacgatt gccgcgggac tctcctgagc catgaaattc
    11941 acgctctcct ggctcaagga gcatctcgac accaaggcga gcgtcgcggc gatcgccgag
    12001 acgctgaccc gcatcggtct cgaggtcgag gaggtgttcg atcccgccgc ggcgctcaag
    12061 ccgttccgcg tcgcggaagt cgtccgctgc gagaagcacc ccaatgccga caagctcagc
    12121 gtctgcgagg tcgacaccgg cgcggaacgg gttcaggtcg tgtgcggcgc gccgaatgcg
    12181 cgggccggga tgaagggcgt ctttgcagcc gtgggcgctt acgtgcccgg catcggcctc
    12241 accctgacca gggcgaagat caggggcgtc gagtcgagcg gcatgctgct ctcggagcgc
    12301 gagctcgagc tgtccgatga gcataccggc atcatcgagc tctcgcgcga tgccaaggtc
    12361 ggcagtccgg ccgccaaggc gctcggcctc gacgacgcgg tgtttcacat cgccatcacg
    12421 ccgaaccgcc ccgactgcct cggcgtgcgc ggcgttacgc gcgatcttgc ggcggccggt
    12481 ctcggcacgc tgaaaaagga taaggtcaag ccggtcaagg gcggcttcgc caatccggtc
    12541 cccatcgcgc tcgaattcga caaggccagc gcgggcgcct gcccggtctt tgccgggcgc
    12601 atcgtgcgcg gcgtcaagaa cggcccctcc cccgattggc tgcagcggcg gctcaaggcc
    12661 atcgggcttc gccccatcaa cgcgctcgtc gatatcacca actacgtttc ctacgaccgc
    12721 gggcgcccgc tccatgtcta cgacgctgac aagctcaacg gcacgatccg cgcgcggatg
    12781 ggccgcttga gcgaaaaatt cctcgcgctc gacggcaagg tttacgaggt tgacgaggac
    12841 atgtgcgtca tcgccgacga tggcgccgtg cttgggcttg gcggggtgat cggcggcgag
    12901 gacacggccg tgaccgaggc gaccaccaac gtcttcatcg agtcggccta tttcgatccc
    12961 aagcgcaccg cgcgcacggg gcggaagctc ggcatccagt ccgacgcgcg cttccgcttc
    13021 gagcgcggcg tcgatcccga attcgtggtg ccgggtctcg aacttgccac tgatctcgtg
    13081 cttcgtctgt gcggcggcgc ggcgagcaag atcgccctcg cgggcagacc gccgaagccg
    13141 aacaagccgt tcaaattcga tcccgccttg gtcgagcggc tctccggact cgatctcgcg
    13201 aggggcgaga tcaagcggct gctcgacgcg ctcggtatcg cgctcgacgg gaaagggaag
    13261 cacctcaaag cagcaccccc ctcatggcgc cccgacatca ccggccctgc cgatctcgtc
    13321 gaggaggtgg tgcgacttaa gggcatcgat gcggttcccg ccactcccat gcgccgggac
    13381 gccgggctgg caaagccggt gctcaccgac gcgcagaggc ggcagcggct cacgcggcgc
    13441 gtgcttgcgg cgcgtggtct cgtcgagacg gtgacctggt ccttcattcc gcccgagcat
    13501 gcggcgttgt tcggaggcgg cgcgcccgag ctgaccttga gcaatccgat ctcgaccgag
    13561 cttgcggcga tgcggccctc gctcctgccc gcgttgatcg cggccgcgca gagaaatcgc
    13621 gacaggggct ttgccgacgg ggcgctgttc gagatcggtc aagcctatcg ggggcctaag
    13681 cccgatgatc agttcgtgac ggcggccggc gttcgcttcg gccgctcttc gctcgcaggc
    13741 tctggccgcc attggtcggg cgaggcccct cccgcggatg tgttcgccgc aaaggccgac
    13801 gcggtcgccg tgctcgcggc gctcgggctc gaccaggcaa acttggccgg cacgcgcgag
    13861 gcgcccgctt ggttccatcc cggccgctcg ggcgcgctta tgctcggacc caagacggtg
    13921 ctgggcgtgt tcggagagtt ccatcccgac cttctcgcga agctcggcat cgatccgccg
    13981 atcgcgggct tcgagctttt cctcgacgcc attccagcaa gccggcgcag aagcgcaagc
    14041 caggcgctcg aggcctcaga cctccagccg gtcaggcgcg atttggcctt cgtgatcgac
    14101 ggagagattg ccgcgggcga tgtggtgcgc gcggcgcttt ccgccgaccg tgcgctcatc
    14161 agcgatgtcg gcgtgttcga cgtgttcacc gggcgaggcg tgcccgaggg caagaagtcg
    14221 ctcgccatcg aggtgacgct gcaaccgcgc gacaagacgc tgaccgacgc cgagatcgat
    14281 gcggtcgccg agaaggtcgt ggccgcggtc aagaaagcaa cgggcggcga actgcgcggc
    14341 tagccccgct cacagtcatg gcccatgcct gtgtcgcgtt actcttcgcg gcagagccgt
    14401 tcacaaaaag tcacgctctc cccttgcggg agagcacgcg cgccatttgg cgcgcggtga
    14461 gggggcagcg gcgaagccgc gtcacgttcg attcccctat ccggctcgcc gcggcagcga
    14521 agcggccctc aggggcacgc aaaattgtgt catccttcgc ggctcgctcc gccctgcctt
    14581 cgccggagcg aagccccggc ttcgcgcagg caggtcgcac ctcaggatga cgtaaagtta
    14641 ttggggaccg acggtgccga agggagacgg agacgacgga ccagccctcc gccgctgcgg
    14701 cacccggcgc ttgaccaaag ccggcttgcc gtagaacggc ttgtcctcgc aggcggacgc
    14761 gatcgtcgcg gcgagcgctt tgtcgggatc ggccggcggc tgccagccct ggcacagcgt
    14821 tccgatttcg ccgccgcgag cggccgcgcc gtcagcgcaa taaagcccag agccgtccgg
    14881 tcgcggcgcg aagcgcacac ctcttgcgtt cttgccgtcg tcgagccccg ccgcgaggtg
    14941 attgagcgca tggatgcgct tcgtcccctc cccggccacg aagcgctcgg gccggctctg
    15001 cgtgttggtc agcgggatcg cctcgatggc ttcgacccgc caggcgccgc cgctgcgggc
    15061 gagatgcact ttggacatca gcccgtaatc gcggcagagc ccgaaacggg caggttccaa
    15121 ggtgcccgga tgaaggaaat tgccgaggcc gtagaagatc agcgactttc cgttgagctc
    15181 aacgccttgc gccacatgcg ggtgatgccc gacgatgaga tcgatcccct tttcctccgc
    15241 cgcgaatttg cgccaatcgt cgagctgccg ctcatcgggc accacgcggc cttcgacccc
    15301 gtaatggagg gagaggatgc ggtaatcggc gggcatcgat gcgagcttgt ccaccaccgc
    15361 cacgaagtcg ggccggtcgc ggtaggaagc ctggcccgct ttgttcggac ccgcgcgcga
    15421 ttggggctga tcgccggtca ggatgccgat ggccgcgaag ccgatacgcg cgccgccgac
    15481 gtcgaggcag cccgggcgga tcgcctcctc gaaattgctg ccggtgccgg caaaggcgat
    15541 cgcgcgctcg gcattggcga ccgcgaggtg atagagcgtc tcctcgatgc cttgcgcgcc
    15601 atagtcgtaa gcgtgattgt tggcgagcga gaacaggttg aagcctgatt ggatcagcgc
    15661 cttgagcgcg tcggggtggc tcctgaagtg gaacgttccc ctgcccctgc cttcaggcgc
    15721 gagatcgttg cggtcggtga cgacggtctc gagattgacg aaggcaaggt cgccgtcgac
    15781 ggcgctcgcg acgccagcaa gcgagtcggc gaagctcgtc acctgcttgc ccttttgcat
    15841 gcccttggca tcgaccttgg cgtctgtcgt gttgaagccg gtgtcgccgg cgagcacgat
    15901 ggtgaggcgg tcgggcgagg cgcgctgcgc gggttcgccc gcgccgatcg cagcagggag
    15961 ctcgggccga tcgcaggggc cggggcgtgt cgcggctgag ggtatgctcg cggcaaaggc
    16021 gagcagcacg gcgcccgccg caaccatgac acgagacttc agccggccgt gcacaagtcg
    16081 cccctcaact cagtgcctgg tccaattcct ggcgaatacg cgcaccgtca tcgaagccca
    16141 gattatggtc gcgggcaaga tgaaccatgg cccgctcggc gagagcgctg atggtgagga
    16201 gcggattgac gccgatcgag cgcgggatca ccgcgccgtc gcagacatag agcccggcat
    16261 ggacgctgtc gcgggccctg ccgtcgaaca cttggcattt gtggttcacg acgccgcttc
    16321 ccgcgtcctc gcccatgccg cagccgccga gcggatgagc ggtcgcgggc ttcgttccga
    16381 cgctcgtcgc ggcaagcggg ctcttgatgt agcgcgcgcc gaccgctttc gccgccttgg
    16441 tcagggcgtc atcgacgcgg gcatagaccg gctggtcggc cacgccaggc cattggacct
    16501 cggcgccgcc attgtgaagc gtgatgcggc ccgcggcttc gtcgtgcgag acgacgaaga
    16561 aggtatgcag atgcgacagc ggaccgtgat agacgccctt gatcaggctc tgcgcggcgc
    16621 cgagcagcct gccgccggcg atgaagaaca cgggcagcag cggggcgagg gcggacggca
    16681 tcaccccttc ctgcaagatc atgctgcgat cgagaacctc gccgtcggca agctcgatct
    16741 gtccggcgac gcaggcgccg atggcatcgc ccaagaattt cggcgggtga cccacgccga
    16801 cggcgttcac tctctcgctg ccgccgagcg caaacgcgat gatgtcgccg ttggcggaga
    16861 atttctcgcc gagccggtcg gagagagaaa gtccatactc gcgtgagcgc agcagaatct
    16921 cggtcgagcc gagcgtgccc gcggcaagca ccacggtcct cgcatcgacg aaggcggggc
    16981 tggcctcccg atcgtcggag gccacgaaat ggacgcgcca gccgccctcc tccttggcga
    17041 tatgcgtcac gctaagctcg gtgaaaatcg ccgcaccatg cgccttcgca tcaggcagat
    17101 aggtcatggc gacggtgttc ttggcgccga cattgcaccc cgagcagcaa tcgccgcaga
    17161 gcgtgcaggc gggctgggcg acgcccgccg gattgacgac atcctcgaag ctcaccacca
    17221 cgggggccgc gaccggggga acgtcgaagg ccgcgcttgc cgcctcgagc gcgcggtatt
    17281 tggtcagcga aggcgcgttc cggtaacgcg acgggcggag catcgcggag gctcgcgcga
    17341 agccgagatc gagcaatccg tcgccgctca ccgcctcggg ccaggccgaa tcggcgaaga
    17401 cgcgcggatc gggtctcagc gcgacgccgg cattgatgag cgagccgccg ccgagcccgc
    17461 agccgaccag cacatgaata tcggcgccga gcctgaaatc gaacaggccc gtgcgcgagc
    17521 ccgaacggag ctttgcgccg ttcagctgaa gctcgcggcg gatctcgggg aggcggctcg
    17581 gaaattcgcc ggtgaggaac tccttgccct tctcgatgac gcagacgctc tggcctgcgc
    17641 gcgcaagccg cgaggcggcg acgccgccgc catagcccga gccgacgacg accacgtcgt
    17701 aggcgcggct gatggcgctt tgcggcgagg agagccgttc ctgcatggcc cctcaatagc
    17761 aaagatgccc aagcgcgccc aagcgcttat cgggggcaat accgggatca attcatttca
    17821 cggtcgcgcg agagccactt tgccgcgttt ccggcaattc ttattgtggc tgcgagattc
    17881 gggttccgga gagaattcca tgatcgcgaa atcggtctgc acggctttgg ccgcggccct
    17941 cctggccgct ctggtgcttg ccttcggcgg atacgaggcg cgcgcggagg cgaccaagac
    18001 cgcggtcttc gcgggcggct gcttctggtg cgtcgaggat gccttcgatc acgtggatgg
    18061 cgtcaccgag acggtctcgg g
//
LOCUS       DASUQP010000180         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_226621,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000180 DASUQP010000000
VERSION     DASUQP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226621"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFY74_07990"
     CDS             <1..300
                     /locus_tag="VFY74_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5931670.1"
                     /translation="AKGVIRTQPLGHHILPGVTRRAVLRLAALEGAALEERPFSVAEA
                     LSAREAFMTAASAFVLPIVAIDGVPIGDGRPGPVANRFRDLYIEEARSSSVGPGA"
     gene            344..1312
                     /locus_tag="VFY74_07995"
     CDS             344..1312
                     /locus_tag="VFY74_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5931671.1"
                     /translation="MEEATSADLRSVERQRVSVRRAVVLANAGAGAFNQALSADLRWR
                     LVDALAQTGIAADIRYVQGGGLREAASRALAEARRGEIDAIIVAGGDGSVRAVASVLA
                     GTDVPLGIVPLGTRNHFARDLRLPLEVEDAAVTIAAGQTRLVDLGEVNGETFINNSSI
                     GIYPYLVIERERRRARHGVAKWLAMAPAFVRMLRNFPRRRLRISAKDFARPYRTPCLL
                     VGNKEYGMELFTFRRRHRLDDGRLWFYVVKPRTPLEFFWMVCRLCFGRIDQARDLDRF
                     ALTEAVVDAKASRLPVALDGEIAIMRTPLHYRSRPHALRVIVPAAP"
     gene            1404..1640
                     /locus_tag="VFY74_08000"
     CDS             1404..1640
                     /locus_tag="VFY74_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX5931672.1"
                     /translation="MFLRPHPSYIKRKLIEFAPELWLALDLLARDTGRSMQDLADEAF
                     ADLLAKYHRPVSLKEALRESARRLPANDDVPPPR"
     gene            complement(1772..1990)
                     /locus_tag="VFY74_08005"
     CDS             complement(1772..1990)
                     /locus_tag="VFY74_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931673.1"
                     /translation="MRILLILILIAAAYVVYQAQTRGCKYDATLINCVFGTSGGEKPA
                     AEMPAPTPAPEPAAPAPETPAPAPAPAQ"
BASE COUNT          368 a          738 c          735 g          405 t
ORIGIN      
        1 gcgaagggcg tgatccgcac ccagccgctc ggccaccaca tcttgccggg cgtgacgcga
       61 cgcgccgtgc ttcggcttgc ggcgctcgag ggggcagcgc tcgaggagcg gcccttcagc
      121 gtggccgagg cgctttccgc gcgtgaggcc ttcatgaccg cggcctcggc cttcgtgttg
      181 ccgatcgtcg cgatcgacgg cgttccaatc ggggacgggc gccccggtcc ggtggcgaac
      241 cgcttccgcg acctctatat cgaggaagcc cgcagctcca gcgtcggccc gggcgcctag
      301 caggccggcc gttcacatcc cgcgaggcgg gacgagtttt cccatggagg aagccacatc
      361 agccgatctt cgttccgttg agcgtcagcg ggtcagcgtg cggcgcgcgg tcgtgcttgc
      421 caatgccggc gccggcgcct tcaaccaggc gctttctgcc gatctcaggt ggaggcttgt
      481 cgatgccttg gcgcaaaccg gcattgccgc cgatatccgg tacgtgcagg gcggaggact
      541 gcgcgaggcg gcctcacggg cacttgccga agcaagacgc ggggaaatcg acgcaatcat
      601 cgtcgcgggc ggcgatggga gcgtgcgcgc cgttgcaagc gtgcttgccg gaacggacgt
      661 gccgctcggg atcgtccctc tcggcacgcg caatcacttt gccagagacc ttcgactgcc
      721 actggaggtc gaggacgcgg ccgtcacgat cgcagccggt cagacgcgct tggtcgatct
      781 cggcgaggtc aacggcgaga ccttcatcaa caactcctcg atcggcattt acccgtatct
      841 cgtcatcgag cgcgagcgac gaagggcgcg tcacggagtc gccaagtggc ttgccatggc
      901 gccggccttc gtccggatgc tgcgcaactt tccgcggcgg cgtctcagga tctcggccaa
      961 ggattttgcc cggccttacc ggacgccctg cctgctggtc ggcaacaagg aatacggcat
     1021 ggagctgttc accttcagac ggcggcaccg gctcgatgac ggcaggctgt ggttctatgt
     1081 ggtgaagccg cgcacgccgc tcgaattctt ctggatggtg tgccgcctgt gttttggccg
     1141 tatcgaccag gcgcgcgacc tcgacaggtt cgcgctcacg gaggcggtgg tcgacgccaa
     1201 ggcaagccgg ctgccggtcg cgctcgatgg cgagatcgcg atcatgcgga cgcccctgca
     1261 ttatcgctcg cgcccacacg ctcttcgcgt catcgtgccc gcggcgccct aaggcggata
     1321 aaccatgctg gttcgcgccg ggttcgcttg agccggggcg aatcgggtat gctggcgtca
     1381 ggcgcgaatc accgggatcg agcatgttcc ttcgcccgca cccaagctat atcaaacgca
     1441 agctcatcga gttcgcgccc gaactctggc tcgcgctcga cctgctcgcc agggacaccg
     1501 gcagaagcat gcaggacctc gccgacgaag ccttcgccga tctccttgcc aagtaccacc
     1561 gtccggtctc gttgaaggag gccttgcggg aaagtgcgcg ccggctcccc gccaatgacg
     1621 acgttccgcc gccgcgctga tccggttttt tttgcggtca gcctctggag aaaagccgaa
     1681 aggaccggcc gaaaggccgg tgccataacg cgccaaagac cgcgcgtttg gaacgcgcgg
     1741 tctttgagtt gtgcctctcg gcgcgaagtc cctactgcgc cggtgccggt gccggtgccg
     1801 gtgtctcggg ggccggtgcc gccggctcgg gtgccggtgt cggtgccggc atctcggctg
     1861 ccggcttctc accccccgaa gtgccgaata cgcaattgat caacgtcgcg tcgtatttgc
     1921 agccgcgcgt ctgtgcctga tacacgacat aagcggcggc aataagtatc aaaatgagaa
     1981 gaatccgcat ttccacccct ccctactgcg cgcgcgtaaa gtatacgaat ggccgtcaaa
     2041 caaaaagggc cgccgcgcgg ctgtgccccg ttttcatggg ccctccatgg accccgtcag
     2101 cccggaaaag cgtgtttctt cgcgcgttat tcgccctttc cgcatcgaca agaggaaggc
     2161 gcatgcatcg cggcatgaac gggcgtggcc aacggatagg tggcaaaggg cggctcacgg
     2221 ccccatttga ctggggcctt gagacg
//
LOCUS       DASUQP010000181         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1045260, whole genome shotgun sequence.
ACCESSION   DASUQP010000181 DASUQP010000000
VERSION     DASUQP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045260"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFY74_08010"
     CDS             complement(<1..505)
                     /locus_tag="VFY74_08010"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5931674.1"
                     /translation="MSTTSANPSAAERAAPARHTFAAADGFKIAYWVDDFTDPWREPE
                     TVLLLHAAMGHSRRWFRWVPRLARRFRVVRMDLRGHGFSQKPKPEEPFSLDQLVGDAA
                     CLVEILGCGPTHVVGLSAGGYVAQQLAIRHAGKVKTLALYASTPGLKNSKAASWVPRI
                     KKIGLKQF"
     gene            complement(471..1307)
                     /locus_tag="VFY74_08015"
     CDS             complement(471..1307)
                     /locus_tag="VFY74_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX5931675.1"
                     /translation="MARLLVCVTFDFDAMSGLMARGLTTPNYISRGEFGPAAIPRILA
                     LLKKYGVKSTFFSPGFTIETYPAQNEAILKAGHEIGHHGWTHLPPHKMTREQEEANLV
                     RGNETIKKLTGKYARGYRSPSWDLSQHTIELLVKHGFLYESSLMGDDYTPYRCRHGDV
                     VDINKPLVFGRPTRLIEMPIAWSLDDFPHFEFLRMETQLKPGLMNANHVIENWINDFV
                     YMKANFEWGVLTYTLHPYVIGRGHRMMALEKLLKAVAEGGVFVTMEEAAREYDERQPF
                     RG"
     gene            1387..2358
                     /locus_tag="VFY74_08020"
     CDS             1387..2358
                     /locus_tag="VFY74_08020"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5931676.1"
                     /translation="MRTLQLLMSGAALACLSLIASAQEYPAKPIRVITPYGAGGASDI
                     VVRAGVHEMSKQLGQAIIVENRTGAGATIGFEAFAASPPDGYTLLGTASSLHGIAAAL
                     YTKTLKHDPNKDVEPIIVFANVSNVLVVNPSVNAKSMKDLIELAKTQPGKLTFVSAGV
                     GTSVHMAGELFKNMAGVQMTHVPYKSSVAALVDLMAGRVDVMFDNIPSALPHIRSSKL
                     RALATTGSKRAAVLPELPTIAEQGLAGYEAGVWIGLLAPAGTPKAIIAKLNAEGQKAV
                     KAPEFMKRMSELGFEVIGGSPEQMRAMIDAEVKRMHPIVRSSGAKPE"
     gene            complement(2407..2521)
                     /gene="rrf"
                     /locus_tag="VFY74_08025"
     rRNA            complement(2407..2521)
                     /gene="rrf"
                     /locus_tag="VFY74_08025"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          468 a          798 c          825 g          451 t
ORIGIN      
        1 ggaattgctt gagtccgatt ttcttgatgc gcgggaccca gctcgcggcc ttgctgttct
       61 tgagaccagg cgtcgaggcg tagagcgcga gcgttttcac cttccccgca tggcggatgg
      121 cgagctgctg ggccacgtag ccgcccgcgg agaggccgac gacatgcgtg gggccgcagc
      181 ccagaatctc gacgagacag gcagcatcgc cgaccagctg gtcgagcgag aacggctcct
      241 cgggcttagg cttctgcgag aagccgtggc cgcgcaggtc catgcgcacg acgcgaaagc
      301 ggcgcgcaag acgcggcacc cagcgaaacc agcggcgcga gtgacccatt gccgcgtgga
      361 gcagcaggac ggtttccggc tcgcgccagg ggtccgtgaa gtcgtcgacc cagtaggcga
      421 tcttgaaacc gtcggcggcg gcaaacgtgt gcctcgccgg cgcggcgcgc tcagccgcgg
      481 aagggttggc gctcgtcgta ctcacgcgcg gcctcctcca tggtgacgaa aacgccgccc
      541 tcggcgacgg ctttgaggag cttctccagc gccatcatgc ggtggccgcg gccgatgacg
      601 tatgggtgca atgtgtacgt gagcacgccc cactcgaaat tcgccttcat gtagacgaag
      661 tcgttgatcc agttctcgat cacatggttc gcgttcatga gccccggctt gagttgtgtt
      721 tccatgcgca ggaactcgaa gtgcggaaag tcgtcgaggc tccaggcgat cggcatctcg
      781 atcaggcgcg tcggtcttcc gaagacaagc ggcttgttga tgtcgaccac gtcgccgtgg
      841 cggcatcgat aaggcgtgta gtcgtcgccc atcaggctgc tctcgtacag gaagccgtgc
      901 ttcacgagga gctcgatcgt gtgctgcgag agatcccagg acggcgatcg gtagccgcgc
      961 gcatatttgc cggtcagctt cttgatcgtc tcgttgccgc gcaccagatt cgcttcctcc
     1021 tgctcgcgcg tcatcttgtg cggcggaaga tgggtccagc cgtgatggcc gatctcgtgc
     1081 cccgctttca ggatcgcctc gttctgcgcc ggataggtct cgatcgtgaa gccggggctg
     1141 aagaacgtgc tcttcacccc atactttttc aggagcgcca ggatgcgcgg gatggccgcc
     1201 ggaccgaact cgccgcgcga tatgtagttc ggcgtggtga ggcctcgcgc catgaggccg
     1261 gacatcgcat cgaaatcgaa ggtcacgcaa acaaggagac gcgccatggg aaatcctttc
     1321 gagagccggt atgcgtcgcg attcttgcac cagctcgcgc ggcggagcta tgctaggttc
     1381 gatgccatga gaacactgca gcttctgatg tcgggcgcag cgctcgcctg cctctcgctc
     1441 atcgcctcgg cgcaggaata tccggcgaag ccgatccggg tcatcacccc ttacggcgcc
     1501 ggcggcgcct ccgacatcgt cgtgcgcgcg ggcgtgcacg agatgtcgaa gcagctcggg
     1561 caagccatca tcgtggagaa ccgcaccggc gcgggcgcga cgatcggttt cgaggccttc
     1621 gccgcctcgc cgcccgacgg ctacaccctg ctcggcacgg cgagcagcct gcacggcatc
     1681 gcggcggcgc tgtacacgaa gacgctcaaa cacgacccga acaaggacgt cgagccgatc
     1741 atcgtcttcg ccaacgtgag caacgtcctc gtcgtgaacc cgtcggtgaa cgccaagtcg
     1801 atgaaggacc tgatcgagct cgcgaagacg caaccgggca agctcacttt cgtctcggcg
     1861 ggcgtaggca cttcggtgca catggcggga gaactcttca agaacatggc aggcgtgcag
     1921 atgacgcacg tgccttacaa aagcagcgtc gccgccctgg tcgacctgat ggccggccgc
     1981 gtggacgtga tgttcgacaa cattccctcg gcgctaccgc atatcaggtc gagcaagctg
     2041 cgcgcgctcg ccaccaccgg ctcgaagcgc gccgccgtcc tcccggaact gccgaccatc
     2101 gccgagcagg ggctcgcggg ctacgaagca ggcgtctgga tcgggctgct cgcgcccgcc
     2161 ggcacgccga aagccatcat cgcgaagttg aatgccgagg gacagaaagc agtcaaagcg
     2221 cccgagttca tgaagcgcat gagcgagctc ggcttcgaag tcatcggggg ctcgcctgag
     2281 cagatgcgcg ccatgatcga cgctgaggtg aagcgcatgc acccgatcgt gcggtcctcc
     2341 ggcgcgaagc ccgaataaca aaaaagcccg gtgctctttc gagcccgggc tttttcgctt
     2401 gagatggcct ggcggtaacc tactttcgcg cgcgcgattg cgcactatca ttggcgcaaa
     2461 cccgtttcac ggtcctgttc gggatgggaa ggcgtggttc caggctgcta tagccgccag
     2521 atagaagatt gaagaagtga ag
//
LOCUS       DASUQP010000182        20011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_773191,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000182 DASUQP010000000
VERSION     DASUQP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 20011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20011
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773191"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1226)
                     /gene="lptG"
                     /locus_tag="VFY74_08030"
     CDS             complement(120..1226)
                     /gene="lptG"
                     /locus_tag="VFY74_08030"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX5931677.1"
                     /translation="MPGISAVGTLARYFSLRFLIAMLAMFLICCVLIFFVDFIEMLRR
                     AGNYSGDVPGVLLAWMSLLRLPSFSELVLPFAVLIGTIGIFLMLSRSSELVVLRAAGI
                     SVWQFTLPAMIVAFLLGVGFVLFYNPIAAVARAEAERLYATAFGRGESLLKTKNAGAW
                     LREDGADGPSVVHALEVLNQGLELTGVTVFQYDRNHGLTERIEAKKAVLKDGRWELDD
                     VWVSAVGQEPAFYKRYLLSTYLTPTQVRDSLGTVFSISFWDLPNFIQIAEKAGLPATQ
                     YRVQYQLLLSRPFLLVTMVLIAATCSLRGFRFGNVQINAVFGLAAGFAFFVFAEISRN
                     FAMAGLTSAVAAAWVPVIVAASLALTVLLYKEDG"
     gene            complement(1235..2416)
                     /gene="lptF"
                     /locus_tag="VFY74_08035"
     CDS             complement(1235..2416)
                     /gene="lptF"
                     /locus_tag="VFY74_08035"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX5931678.1"
                     /translation="MTIFGRYLFRQTANALLVILLTLTLVVWLATALKELNLITSRGQ
                     GILLFLQMTMLSLPSLMALIAPNAMLMAALYTLDRMNGDSELIVMTASGAPIWRIGAP
                     LLALASIVAIAVLIANLIVTPASMRALRSFITHVRADLISQVLQPGRFSSPEGGLTFH
                     IRDRSLNGDLLGLLVHDERDDKQVMSYLAERGRIIDNDEGSYLIMLRGYVHRYNNEAE
                     EKDVQIVAFDQNMLDLSEFSPKDSGGKELRPREAYTSDLFSPDLTNAAALRNYPQLRA
                     ELHERLATPLYPLVFAFVAIAFLGHARTTREGRWGQILAAFGIAIGLRAAGIAAGNMV
                     ALSPWAIGLVYGLPVGAIIIAAWAAHAKMVPQLRSRLSLGAKFQPKTIRFWAGRGMQT
                     T"
     gene            2697..4220
                     /locus_tag="VFY74_08040"
     CDS             2697..4220
                     /locus_tag="VFY74_08040"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5931679.1"
                     /translation="MSDQANVSFESTTAPAEGVVVIFAEEGRKLGPLAQDLDKTSKGL
                     LSKAAEITGFKGKKEQTVDLLAPQGLKFARLVLVGMGKASAYRSEDWLNLGGTVRGLL
                     SGKEAPAAHIFVEAADGDATPADIASFALGLQLRGYNFRKYKTKSRKKNGEGGETNDR
                     TLKKIVIHSADPKAASRAFAPERAMAEGVALARDLVNEPANVLGPAEFATRARALSKR
                     GVRVEVLGPKVLRKLGMNALLAVGQGSARPSHVVVMQWKGAGAKGEPIAIVGKGVTFD
                     TGGISLKPAAGMEEMKGDMAGAACVVGLMHELAARKAKVNAVGVIGLVENMPSGTAQR
                     PGDVVTAMSGQTIEVLNTDAEGRMVLADCLWYVQDRFKPKAVINLATLTGAVMVALGK
                     EHAGLFSNNDELAGWLIEAGEATGEKLWRLPLGPKYDKMIDSKLADMKNTGGKWGGSI
                     SAAQFLQRFIKEGTPWAHLDIAGTAMSSVDSEINRSWGSGFGVRLLDRLISEHYERH"
     gene            4409..5239
                     /gene="mazG"
                     /locus_tag="VFY74_08045"
     CDS             4409..5239
                     /gene="mazG"
                     /locus_tag="VFY74_08045"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX5931680.1"
                     /translation="MSDGPEKRYTLDDLLAVMARLRDKDAGCPWDIAQSFGTIAPYTI
                     EEAYEVADAIAREDLDALKEELGDLLLQVAYHAQMASEERRFGFDDVVDTITRKMVRR
                     HPHVFGEASRDEFLTKNMWGRIKEAEKAERGSTSRRSRFDDVPLALPALARAVKLQKR
                     AAEVGFDWPSVAPVVAKAEEELTELKGAVAARDESDRGPATRAREELGDLLFAVANIA
                     RHLGVDPEAALRDANAKFLRRFATVEEALAKDGRKAEDATLEEMDRLWDEAKAAERKI
                     "
     gene            complement(5260..6573)
                     /gene="hflX"
                     /locus_tag="VFY74_08050"
     CDS             complement(5260..6573)
                     /gene="hflX"
                     /locus_tag="VFY74_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5931681.1"
                     /translation="MVLLPVLGGRRGRAPRAQRADTVARSPQARLEEAIGLAAAIDLD
                     VVANGLVPLETPRPATLFGSGKAEELEGVVRAEDVGLVIVDHPLTPVQQRNLETAWGA
                     KVLDRTGLILEIFGRRARTREGRLQVELAHLVYQKSRLVRSWTHLERQRGGFGFLGGP
                     GETQIEADRRIIGERIDSIRKELERVRRTRGLHRASREREPYPSVALVGYTNAGKSTL
                     FNALTGASVLAEDILFATLDPTIRSLPLPGGSKAVMSDTVGFISDLPTTLIAAFRATL
                     EEVLEADLILHVRDIAGPTSGSEREDVNAVLSELGIEADAEPGRLMEVWNKADLLKDP
                     AVRQENSEASRTGAVLVSAVTGYGLDALRDEIERRLNLRSETLEIALKPEEGALSHWI
                     HENCEVIKRRAMGEGITALRVRVAPEKRERLARLAGAARLKLAAE"
     gene            complement(6667..6921)
                     /gene="hfq"
                     /locus_tag="VFY74_08055"
     CDS             complement(6667..6921)
                     /gene="hfq"
                     /locus_tag="VFY74_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX5931682.1"
                     /translation="MATERAQSLQDTFLNHVRKSKTPLTIFLVNGVKLQGVVTWFDNF
                     CVLLRRDGHSQLVYKHAISTIMPGQPIQLAEISDAEGAAE"
     gene            complement(7092..8489)
                     /locus_tag="VFY74_08060"
     CDS             complement(7092..8489)
                     /locus_tag="VFY74_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5931683.1"
                     /translation="MASDILVVDDEADIRDLVAGILEDEGHSTRLAKDSDETLKAIEE
                     RRPSLVILDIWLQGSKLDGLELLDRIKKAHPDLSVIIISGHGNIETAVAAIKRGAYDY
                     IEKPFKADRLVLITARALEASRLRRENRELRERSTYSFEMIGRSAAINQLRQAIDKVA
                     PTNSRVLITGPSGCGKELAARVMHAKSTRAEGPFVVLNAAAMVPDRMELELFGTEEGA
                     GAGPRKVGALEEAHNGTLYIDEIADMPLETQAKILRVLVEQRFQRLGGGPKVHVDVRV
                     VSSTSHELQKEMSAGRLREDLYHRLNVVPIRVPGLAERREDLPELVRYFVQTISVASG
                     LPPRRIGDDAMAVLQSHDWPGNVRQLRNNVERLMILVGGDPDSVITADMLPDEIVSPV
                     PLSANGAGGEHLMSLPLRDAREIFEREYLLAQVNRFGGNISRTAEFVGMERSALHRKL
                     RALGVNSNERGTAFR"
     gene            complement(8479..10896)
                     /locus_tag="VFY74_08065"
     CDS             complement(8479..10896)
                     /locus_tag="VFY74_08065"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX5931684.1"
                     /translation="MAFRGVLQSMYAGAERSALGGSRETMTHPSTRTKDAAPAGTAAG
                     WDEGRRRLPFTLGLITAVLSLCSGLATYVILTGLTPIKPTDNVVVVVLFINLVPVLAM
                     IAIIAWQVTGLWLARQRQAAGAQLHVRIVSLLSVIALVPAILLAIFASISLGRGLDYW
                     FSVRTQAIVQNSMEVAEAYLEEHEQVIRANTVAMARYIDEGAGLARSLPQKFGGFLST
                     QAAMNGFPVAYLIDGEGKVLATAAELRDLPYKKPPEEVMARAKKGPPVPISLKDTNMV
                     GAIKSLNNFTDTYLYVMRPVNLRVLQHLSATRANVAEYQGLEQRRTNFQMAYGLIYLA
                     LALTLLASAIWIGMWFANRLVAPIRQLMGAAEQISRGNLGVNVDVRPADGDLAVLGST
                     FNTMTAELKSQRDELIGANSKLDERRQFMEAVLSGVTAGVVGVDSHGVVTLVNRSAET
                     LLEMKEAVLVGQPLAKAVPEFAQVLKRAQKQGKRLVTDQVTLRRQGSERNFAVRVTSE
                     GQDQDVQGFVVTFDDMTELVSAQRSTAWADVARRIAHEIKNPLTPIQLSAERIRRKYG
                     NSITKDREVFDQCTNTIIRQVSDIGRMVDEFSAFARMPKPVMERHDVRDIVREAVFLF
                     QVSRPDIAFELDLPETPVVALSDRRLLSQAVTNLVKNASEAIDSAVEGDASRAGSGRI
                     VTKVRTRANRVQISVLDNGCGLPAENRERLVEPYMTTRTKGTGLGLAIVQRITEQHAG
                     TLTLADGPKRNGKVEGASVRIDFPTGEREGTRTEEVMPEQGASVPAPAPEHPAEAEEE
                     EGVTHGV"
     gene            complement(11077..12519)
                     /gene="ntrC"
                     /locus_tag="VFY74_08070"
     CDS             complement(11077..12519)
                     /gene="ntrC"
                     /locus_tag="VFY74_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX5931685.1"
                     /translation="MTKGNILIADDDAAIRTVLNQALARAGYAPRVTGNAATLWRWIS
                     QGEGDLVITDVLMPDENAFDLIPRVKKVRPDLPIIVMSAQNTFMTAITAAERGAYEYL
                     PKPFDLNELVAVVGRALAEPRRKAQRLPAEAPEGLPLIGRCAAMQEIYRVLARLTQTD
                     LTVMITGESGTGKELVARALHEYGKRRNGSFVAINMAAIPRELIESELFGHEKGAFTG
                     AQNRHIGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTNVRII
                     AATNRDLQQQIDQGLFREDLFYRLNVVPLRLPPLRERLEDIPDLVRHFLAIAAREGLP
                     PKGFEPAAIDRLKRYHWPGNVRELENLVRRLSAIYSQDIATVDMIENELASPARAPFT
                     NAAEATGDLSEAVERYLAEYFMSFGRDLPPIGLYGHILRKVELPLLTAALAATRGNQI
                     RAADLLGLNRNTLRKKIRDLGIKVIRSQGG"
     gene            complement(12526..13668)
                     /locus_tag="VFY74_08075"
     CDS             complement(12526..13668)
                     /locus_tag="VFY74_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5931686.1"
                     /translation="MGSAKKLNARRVVEFPPIEIALLGALPHPLLVIGEKLAIDYANT
                     AAEDFFQMSAGVLCLRALTEIVAFGSPLIALVEQVQRNGATVNEYGVELGSVRFEAPK
                     LVDLYGGPVAELSGRVLVMLQQRSMAQMIERQLTHRGAARSVSGLAAVLAHEIKNPLS
                     GIRGAAQLLESERVDSDRTLTQLICAESDRICKLVDRMQIFGDERPLQREPVNIHDVL
                     DHVKRIAATGFARKVKILENYDPSLPPVPGNRDKLIQAFLNLVKNAAEAIGEERADGQ
                     ITLTTSFRPGVRLSVPGSQARVSLPLEITVEDNGAGVSADLMPHLFDPFVTTKPSGTG
                     LGLALVAKIIGDHGGIIECDSRPRLTVFRALMPMAEGATPPRTVEG"
     gene            13860..15044
                     /locus_tag="VFY74_08080"
     CDS             13860..15044
                     /locus_tag="VFY74_08080"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX5931687.1"
                     /translation="MTVAALIVAAGRGSRAAGDRGRPKQYCPIGGAPMLTHTLAAFAH
                     HPRVDDILVVIHPDDAALYGEASEPYAARLRSPIAGGARRQDSVRAGLEALANVAPSS
                     VLIHDAARPFVPAELIDRVIDSLKVHQGALPALPVTDSLKRAEAGRVTGTVARESVWQ
                     AQTPQGFAFGRILAAHLAAAEREPDRDFSDDSGIAEWFGLDVALVEGAESNRKLTTAE
                     DLRIADELLRPKRAKDMGTIRVASGYDVHALGPGNAVTLCGVSIPNPKSLVGHSDADV
                     GLHALTDALLGTIAEGDIGVHFPPSDARWRGAASELFLKHAAAMIVERGGTIVHVDVT
                     LICEGPRIGPYRDRMRERIAGMLGLDVAQVSIKATTNEGLGSIGRGEGIAAIATATVS
                     LP"
     gene            15041..15544
                     /locus_tag="VFY74_08085"
     CDS             15041..15544
                     /locus_tag="VFY74_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX5931688.1"
                     /translation="MTDRVEIDAKLLARTAKLLDACRERNETVTAAESCTGGLVAATL
                     TAIPGSSDVFERGFVTYSNSAKSELLGVPYWLIERHGAVSEDVARAMAGGALLHSHAS
                     LAVAVTGIAGPNGGTEEKPVGFVCFAAGRRDAPIQSECVSFGDLGRTEIRRRSVERAI
                     DLLLSLL"
     gene            complement(15533..16009)
                     /locus_tag="VFY74_08090"
     CDS             complement(15533..16009)
                     /locus_tag="VFY74_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX5931689.1"
                     /translation="MHVYETTHPVAHSADEMFALVARVEDYPRFLPLCEALEIKRRDV
                     SDGKEVIVAKMTVGYGLIRESFTTKVHLDPPARKILVEYLDGPFTFLENRWHFQPRGS
                     HSCDVAFYIAYAFSSRLFERLVGRLFAKAVERYTRAFEARADAVYGRNAAALASQQ"
     gene            complement(16034..16996)
                     /gene="lipA"
                     /locus_tag="VFY74_08095"
     CDS             complement(16034..16996)
                     /gene="lipA"
                     /locus_tag="VFY74_08095"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5931690.1"
                     /translation="MVTVIERLDRVTPRPRHPEKARLPDTPVLRKPDWIRVRAPGARA
                     FEATRAILRDHRLTTVCEEASCPNIGECWGKRHATFMILGDTCTRACAFCNVRTGRPG
                     ALDSEEPARVAQAVAALDLAHVVVTSVDRDDLADGGAGHFAATIRAIRAACPAASVEV
                     LTPDFRRKDGALEIVLDAVPDVFNHNVETVPSLYRRIRPGADYRHSLRLLSRAKSLRP
                     DLFTKSGIMVGLGETEGEVVAVMDDLRAAAVDFLTIGQYLQPTRKHAAIDRFVTPEEF
                     KSYKRIAEDKGFLLVASSPLTRSSYHAPQDFARLKAARRAARGA"
     gene            complement(17021..17302)
                     /locus_tag="VFY74_08100"
     CDS             complement(17021..17302)
                     /locus_tag="VFY74_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5931691.1"
                     /translation="MDNAQTLLAMPGVGFIGMLIIGIIAGYIAEKVTSSDHGLLTNLL
                     VGIAGSFVGGNLASVLNVEFYGWLGNLLVASIGAILILWIWRAIRGPSV"
     gene            complement(17383..18789)
                     /gene="lpdA"
                     /locus_tag="VFY74_08105"
     CDS             complement(17383..18789)
                     /gene="lpdA"
                     /locus_tag="VFY74_08105"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5931692.1"
                     /translation="MTATRFDLAIIGAGPGGYVAAIRASQLGLKTAIVEREHLGGICL
                     NWGCIPTKALLRTSELYQAMRHASSFGLATDNVRFDLARVVERSRKAAERLSNGVAYL
                     MRKNNIAVIDGTARLMGQGRIAVTGKTGETLPDIEAAHIIVATGARARTLPGLEPHGR
                     LIWTYREAMVPQSFPQSLLVIGSGAIGIEFASFYRSLGAEVTVVEMLPRILPAEDAEI
                     SGLARKAFARQGIAIRTDTRVERLVKGADAVTAQLRKPDGNSEELRASRVILAIGITG
                     NVEGLGLEQQGVKVEKGHIVVDEWYRTAASGIYAIGDVAGPPWLAHKASHEGVLCVEK
                     IAGVEGVHLLDRNAIPSCTYSAPQVASVGLTEEAARAAGRDLKIGRFPYSANGKAIAL
                     GETEGLVKTIFDARTGALLGAHIIGAEATELIHSLVIAKTLETTEAELMAAIFPHPTL
                     SEMIGESVLDAYGRALHI"
     gene            complement(18786..19409)
                     /locus_tag="VFY74_08110"
     CDS             complement(18786..19409)
                     /locus_tag="VFY74_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5931693.1"
                     /translation="MDPAALFRPATKADASALAVLVDIAGEGMPAYMWSTLAAPGQSV
                     LEFGRERAQRDSGGFSWRNAVVAEVNGEVAASLVGYRLDDPYDLGALADMPEIVQPLV
                     KLEATAPGSWYVNVLATFPEFRGRGIGAKLLEIAERTGSETGAPALSVIVASWNATAL
                     RLYRRAGYATVASEPAIAFPGSHREGDWVLMVKSLANRRLTGEARGQ"
     gene            complement(19421..>20011)
                     /locus_tag="VFY74_08115"
     CDS             complement(19421..>20011)
                     /locus_tag="VFY74_08115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5931694.1"
                     /translation="DALLAARARLNALAPQQGPRAFKLSVNDFIIKAMAMALQGVPAA
                     NASWTEQGLLRHRASDIAVAVALEGGGLHTPVIRDAELKSLSEISNEMRDLAARARSK
                     RLAPHEYQGGSTTISNLGMYGIDRFDAVINPPQASILAVGRGEKRAVVKDDALKIATM
                     MSVTLSVDHRVIDGALGAELLAAFKAHIEDPVTMLV"
BASE COUNT         3616 a         6294 c         6596 g         3505 t
ORIGIN      
        1 ggcggcgaga atgcaggcaa aaagcgcccg atgcaggcgc gcaaacaccc tgcctttaga
       61 cgcggcaagg ccgggctcag cccggcgcat gcaggtcaat tttgcgcgcg cgccggccat
      121 catccgtcct ctttgtacag aagaacggtg agcgcgagcg aggccgcgac gatcaccggc
      181 acccatgcgg cagcaaccgc cgaggtgaga cccgccatgg caaagttgcg cgagatttcg
      241 gcgaacacga agaaggcaaa accggcagca agtccgaaca cggcgttgat ctggacgttg
      301 ccgaagcgga atcccctgag cgaacaggtg gcggcaatca gcaccatggt gacgaggagg
      361 aacggccgcg acagcaagag ctggtactgc acgcggtatt gcgtggcggg aagtcccgcc
      421 ttttcggcga tctggatgaa gttgggcaag tcccagaacg agatcgagaa caccgtgccg
      481 agactgtcgc gcacctgggt cggcgtgaga taggtgctga gcagatagcg tttgtagaac
      541 gcgggctctt ggcccacggc gctcacccag acgtcgtcga gttcccagcg gccatccttg
      601 agcaccgctt tcttggcctc gatgcgctcg gtcagaccgt ggttgcggtc gtattggaac
      661 accgtgacgc ccgtgagctc gagaccctgg ttgagcacct cgagcgcatg gacgacggac
      721 gggccgtcgg caccgtcctc gcgcagccaa gcaccggcat tcttcgtctt gagcaacgac
      781 tcgccgcggc cgaacgcggt ggcgtacaaa cgttccgctt cggcgcgcgc gaccgccgcg
      841 atcggattgt agaacagcac aaacccgaca ccgagcagga aggcgacgat cattgccggg
      901 agcgtgaact gccacaccga aatgccggct gcacggagca ccacgagttc ggaggagcgg
      961 ctcagcatca ggaagatgcc gatggtgccg atgagaacgg cgaaggggag aacgagttcg
     1021 gagaaggacg gcagacgcaa gagcgacatc caggcgagga ggacacctgg aacgtcgccg
     1081 gaatagttgc cggcacgccg gagcatctcg atgaagtcga cgaagaaaat cagcacgcaa
     1141 cagatcagga acatcgccag catggcgatg aggaagcgca gcgaaaagta gcgcgcaagc
     1201 gtgcccactg cactgatgcc tggcatctgc gctctcacgt cgtttgcatc cctctaccgg
     1261 cccaaaatct tatcgttttt ggctggaact tagcccctaa gctcaacctg gaccgcagct
     1321 gtggaaccat tttagcgtgc gccgcccagg ccgcaatgat aatcgccccc acgggaaggc
     1381 cgtaaacaag cccgatcgcc cagggagaca gggcgaccat gttgccggcg gcgatgcctg
     1441 ccgcgcgcaa gccgattgcg atgccgaaag ccgcgagaat ctgcccccag cgcccctccc
     1501 gcgtggtgcg ggcatggccg agaaaggcga tagccacgaa ggcgaagacg agggggtaga
     1561 gcggcgttgc gaggcgttca tgcagttccg ctctgagctg aggataattc cgcagcgccg
     1621 cagcgttggt caggtcagga ctgaaaagat cgctggtata agcctcccgc ggccgcagct
     1681 cttttccccc gctatccttt gggctgaact ctgagagatc gagcatattc tggtcgaaag
     1741 caacgatttg aacgtctttt tcttccgctt cattgttgta ccggtggacg tagccccgga
     1801 gcatgatcag gtaagacccc tcgtcgttgt cgatgatgcg gccgcgttcg gcgaggtagc
     1861 tcatcacctg cttgtcgtcg cgctcgtcat ggacgaggag tccgagcaaa tcgccgttca
     1921 ggctgcgatc gcggatgtgg aaggtcagcc ccccttcggg gctggaaaaa cgtcctggct
     1981 gaaggacttg cgagatcagg tcggcgcgca catgggtgat gaaactgcgt aaggcccgca
     2041 tgctcgccgg cgtcacgatc agattggcga tcaggaccgc gatggcgacg atcgaggcga
     2101 gggcgaggag gggggcgccg attcgccaga tgggcgctcc cgaagccgtc ataacgatca
     2161 gctcgctgtc gccattcatc cggtcgaggg tgtagagcgc cgccatcagc atggcgttcg
     2221 gggcaatcag cgccatcagg ctcgggaggc tcagcatggt catctgcagg aagagcagga
     2281 tgccctgacc gcgcgaggtg attaagttca attctttcaa tgctgtagca agccagacga
     2341 cgagcgtcag ggtgagcaga atgacgagca gagcgttcgc cgtctgccga aacaaatagc
     2401 ggccgaagat ggtcatcgcc gtcccgcaat tcccccgcta tggcgccttg gcgaagcctg
     2461 ctcagagcaa cgccaacccc ctggtgccaa agacgattct caccagttca agatggcgga
     2521 atttcggcct ttttggaatg gcttgctttc gtctgagact ggatttacca aaagccgctg
     2581 ttatttcctg ccgctgttgc aatactgcat cagtcagcgc ttccgacgct acgccgggga
     2641 cgccgtgccg atcgattgcc cgagccaagc ggcgagggca gcgaaggagt tttttggtga
     2701 gcgatcaagc caacgtgtcc tttgaaagca cgaccgcgcc ggccgaaggc gtcgtcgtga
     2761 tcttcgccga agaaggacgc aagcttggtc cattggccca ggatctcgac aagacatcga
     2821 agggtctcct gtccaaggcc gccgagatca ccgggttcaa aggcaagaag gaacagaccg
     2881 tcgatcttct tgcgccgcaa ggactgaaat tcgcgcggct cgttctcgtc ggcatgggga
     2941 aagcgagcgc ctatcgcagc gaggactggc tcaatctcgg aggcaccgtg cgggggcttc
     3001 tcagcggcaa ggaggccccc gccgctcaca ttttcgtcga ggcggccgat ggcgacgcga
     3061 cgcccgcgga cattgcaagc tttgcgctcg ggcttcagct ccgcggatac aatttcagaa
     3121 aatacaaaac caagtcgcgc aagaaaaacg gcgaaggcgg ggaaacaaac gacaggacac
     3181 tgaagaagat cgtgatccac tcagccgatc ccaaagcggc aagccgcgcc ttcgctcccg
     3241 agcgtgccat ggccgagggc gtggcgctcg ctcgcgatct cgtcaacgaa ccagccaacg
     3301 tgctcggccc cgcagaattc gccacgcgcg caagggcgct ttcgaagcgc ggcgttcgcg
     3361 tggaggtgct cggcccgaaa gtcttgcgca agctcggcat gaacgcgctg ctcgccgtgg
     3421 gacagggaag cgcgcggcca agtcacgtcg ttgtcatgca gtggaaaggc gcgggcgcga
     3481 agggcgaacc gatcgccatc gtcgggaagg gcgtcacctt cgataccgga ggcatctcgc
     3541 tgaagcccgc tgccggcatg gaagagatga agggcgacat ggcgggagcg gcttgtgttg
     3601 tcgggctgat gcacgagctg gcggcgcgca aggcgaaggt taacgccgtc ggcgtcatcg
     3661 gtctcgtcga gaacatgccg agcggaaccg cgcagcgtcc cggcgacgtg gtcaccgcca
     3721 tgtcgggaca gaccatcgag gtgctcaaca ccgacgccga gggccgcatg gtgctcgccg
     3781 actgcctgtg gtacgtgcag gatcgcttca agccgaaagc ggtgatcaat cttgcgacgc
     3841 tcaccggcgc cgtcatggtc gcactcggca aagagcatgc cggcctgttc agcaacaacg
     3901 acgagcttgc cgggtggttg atcgaagcgg gcgaggccac cggggagaaa ctgtggcgtc
     3961 tgcctctcgg gccgaaatat gacaagatga tcgactcgaa gctcgccgac atgaagaaca
     4021 caggcggcaa gtggggcggc tcgatctccg ccgcgcagtt cctccaacgc ttcatcaagg
     4081 aaggaacccc gtgggcgcat ctcgatatcg ccggcacggc gatgtcgtcg gtcgacagcg
     4141 agatcaatcg aagctgggga tcgggatttg gcgtgcgcct gctcgaccgg ttgatttccg
     4201 agcattacga gcgacactga gaggccgtga gagcccgttg gctttgcgac ccagcgcgac
     4261 cgggccaaaa actaattttc cataatatag cttatgcgaa tctcccatct ggggccgaca
     4321 attggcgtat ggctatccat cggatgctaa agcttaattc tgcgcaggtc gctttcgggg
     4381 cggttgcccg gcagcaccgg gaagagacat gagtgacggg ccggagaagc gctacacgct
     4441 cgacgatctg ctcgccgtca tggcgcggct gcgcgacaag gatgcgggct gtccatggga
     4501 catcgcgcag agcttcggca cgatcgcgcc ctacacgatc gaagaagcct acgaggtcgc
     4561 tgacgcgatc gcgcgcgagg atctcgatgc gctcaaggaa gaactcgggg acctcctcct
     4621 ccaggttgct tatcacgctc agatggcgtc ggaggaacgg cgcttcggct tcgacgacgt
     4681 cgtagacacg atcacgcgga agatggtgcg acggcatccg cacgtgttcg gcgaggccag
     4741 ccgggatgag ttcttaacga agaacatgtg gggccgcatc aaggaggcgg agaaggccga
     4801 gcgcggttcc acgagccggc gctcgcgctt cgatgacgtg ccgctagcgc tccccgcgct
     4861 ggcgcgcgcg gtcaagcttc aaaagcgggc cgcggaggtt ggcttcgatt ggccgagcgt
     4921 cgccccggtg gtcgccaagg cggaagagga actgaccgaa ctcaaaggtg cggtcgccgc
     4981 gcgcgatgag tcggataggg gcccggcgac gcgcgcacgt gaggagctcg gcgatctcct
     5041 ctttgccgtg gcgaatatcg cgcggcatct cggcgtcgat cccgaggctg cgcttcgcga
     5101 tgccaacgcg aaattcctgc gccgcttcgc aaccgtcgaa gaggccctgg cgaaggacgg
     5161 gcgcaaggcc gaagacgcaa cgctcgagga gatggatcgg ctctgggacg aggccaaggc
     5221 cgccgaacgg aagatttagc gccggagccg gactccgcgc tattccgccg cgagcttgag
     5281 ccgcgccgcg ccggcgaggc gggcaagcct ctcccgcttt tccggcgcca cgcggacgcg
     5341 aagcgccgtg atcccctcgc ccatggcgcg gcgcttgatc acctcgcaat tctcgtggat
     5401 ccagtgcgac agcgcgccct cctcgggctt gagcgcgatc tcgagcgtct cgctcctgag
     5461 attgagcctc cgctcgattt cgtcgcgcaa ggcatctagg ccgtagccgg ttacggccga
     5521 gacgagcacg gcgcccgtgc gcgatgcctc gctgttctcc tgccgcacgg cgggatcttt
     5581 gagaaggtcg gctttgttcc agacctccat cagccggccc ggctccgcgt ccgcctcgat
     5641 gccaagctcg gacagcaccg cattaacgtc ttcgcgctcg cttccgcttg tgggcccggc
     5701 gatatcgcgg acatgcagga tcaggtcagc ctccaggact tcttccagcg tggctcggaa
     5761 ggcggcgatc agcgtggtcg gcaaatcgga aatgaagccc acggtatcgg acatcaccgc
     5821 cttcgagccg ccgggcagcg ggagcgacct gatcgtcggg tcgagcgttg cgaacagaat
     5881 gtcctcggcg agcaccgagg ctccggtcaa ggcgttgaac agggtcgact tgccggcatt
     5941 ggtgtagccc acgagcgcga ccgacggata gggctcgcgc tcccgcgacg cccgatgcaa
     6001 gccgcgcgtg cggcgcaccc gctcaagctc tttgcggatg ctgtcgatgc gctcgccgat
     6061 gatgcgccgg tcggcttcga tctgggtctc gcccgggccg ccaagaaagc cgaagccccc
     6121 gcgctgtcgc tcgagatgcg tccacgacct gaccagccgg ctcttctgat agacgagatg
     6181 ggcgagttcg acttgcagac gcccttcccg tgtccgcgct cttcggccga agatctcgag
     6241 aatgagcccg gtgcggtcga gcaccttggc tccccacgcg gtttcgagat tgcgttgctg
     6301 caccggggtg agcgggtggt cgacgatgac gagcccgaca tcctcggcgc gcacgacacc
     6361 ttcgagttcc tcggccttgc cggacccgaa cagcgtcgcc ggtcgcgggg tttcgagcgg
     6421 aacgagcccg tttgccacca catcgagatc gattgcagcg gcaagcccga tcgcctcttc
     6481 aagacgcgct tgcggcgatc gcgcgacggt atcggcacgc tgtgcgcggg gagccctccc
     6541 ccgcctgccg ccaaggacgg gcaagagcac catggccctc atcggccctt gccgcgctcg
     6601 gcggctcggc ccgcgcgcgg tccgccggcc cttccgcttc agcttcttcg ggtcggtcgg
     6661 acgcaattac tcagcggcac cttccgcgtc ggagatctcc gcgagttgaa tgggctgacc
     6721 cggcatgatc gtcgagatcg cgtgcttgta gacgagctgc gaatgtccat cgcggcgcag
     6781 caggacgcag aaattatcga accacgtcac cacgccctgc agcttgacac cattgacgag
     6841 aaatatggtg agcggtgtct tgctcttgcg gacgtgattg agaaaggtgt cttgcaggct
     6901 ttgcgcgcgt tctgttgcca tgggcttccc cgtccctttt ggtcacgctt acgctgttct
     6961 ttggcgcggg tctctctgcc cgggcctctg acggctatta gacgcgatta cgaggtcgtc
     7021 ccgcaagtag ttctgatgcc aaagagtttc gtcccgctcg cctctccgtc cctccccgcg
     7081 accgcaaggg atcagcgaaa ggccgtaccc cgctcgttcg agttcacccc cagcgctctg
     7141 agtttgcggt gcaacgccga gcgttccatg cccacgaact ccgcggtgcg cgagatgttg
     7201 ccgccgaacc ggttgacttg ggcgagaaga tactcgcgct cgaagatctc gcgcgcgtcg
     7261 cgcaacggga gcgacatcag atgctcgccc cctgccccat ttgccgatag cgggaccgga
     7321 gagacgatct cgtcgggaag catgtcggcg gtgatgaccg aatcgggatc gccgcccacg
     7381 agaatcatga gccgctcgac gttgttgcgc aattggcgga cgttgccggg ccagtcgtgc
     7441 gattgcagca cagccatggc gtcgtcgccg atgcggcgcg gcgggaggcc cgatgccacc
     7501 gaaatggtct gcacgaaata gcgcaccagc tccggaaggt cctcgcgccg ctcggccaag
     7561 cctggaactc tgattggtac gacattgaga cgatgataga gatcttcgcg caggcggccg
     7621 gcgctcatct ccttttgcag ttcgtggctc gtcgaggaca ccacgcgcac atcgacatgc
     7681 accttgggtc cgccgcctag ccgctggaag cgctgctcga ccagcacgcg caggatcttg
     7741 gcctgggtct cgagcggcat gtcggcaatc tcgtcgatat agagggtacc gttatgggcc
     7801 tcctcgagcg cgccgacctt gcgcggcccc gcgccagcgc cctcctcggt tccgaacagc
     7861 tcaagctcca tgcggtcggg caccatggcc gcggcgttca acaccacgaa gggaccctcg
     7921 gcgcgggtcg acttggcgtg catcacgcgc gcggcaagct ccttgccgca gcccgacggc
     7981 ccggtgatca gaacgcggct attggtcggg gccaccttgt cgatcgcctg gcgcaactgg
     8041 ttgatcgcgg ccgagcggcc gatcatctcg aaggaatagg tgctccgttc gcgcagctcg
     8101 cgattttcgc ggcgaagccg cgaagcttcg agcgcgcggg cggtgatgag cacgagccgg
     8161 tccgccttga acggcttctc gatatagtcg taggcgccgc gcttgatggc agcgaccgcg
     8221 gtctcgatat tgccgtggcc gctgatgatg atgaccgaga ggtcggggtg ggccttcttg
     8281 atgcggtcga ggagctcgag gccgtcgagc ttggagcctt gcagccaaat gtccaggatc
     8341 acgagcgagg gacgccgttc ctcgatcgct ttcagcgtct cgtcgctgtc cttggcgagc
     8401 cgcgtcgaat gaccttcgtc ctcgagaatg cccgcgacta ggtccctgat atcggcttcg
     8461 tcatcgacga ccagaatgtc agacgccatg ggtcactcct tcttcctcct cggcctcggc
     8521 gggatgctcg ggggcgggcg cagggacgga ggcgccttgc tcgggcatga cctcctccgt
     8581 cctcgtccct tcccgctcgc ccgtcggaaa atcgatacgc accgaagccc cttcgacctt
     8641 gccgttgcgt tttggcccat cggccagcgt gagcgtcccg gcatgctgct cggtaatccg
     8701 ctgcacgatc gcaagcccga gtcccgtccc cttcgtgcgc gtggtcatgt agggttcgac
     8761 cagccgctcg cgattctccg cagggagacc gcagccattg tcgaggacgc tgatctgcac
     8821 ccggtttgct ctggtgcgga ccttggtcac gatacgtccc gagcccgcgc gggaagcgtc
     8881 tccctcgacg gccgagtcaa tcgcctcgct tgcgttcttg acgagattgg tgacggcctg
     8941 ggacaggagg cgccggtcgg acagcgcaac caccggcgtc tcgggcagat caagctcgaa
     9001 ggcgatgtcg ggtctcgaga cctggaacag aaagaccgcc tcgcgcacga tgtcgcgcac
     9061 gtcgtgacgc tccatcaccg gtttcggcat gcgggcgaag gccgagaact catcgaccat
     9121 gcggccgata tccgacacct gacgaatgat ggtgttggtg cactgatcga agacctcgcg
     9181 gtccttggtg atcgaattgc cgtatttacg gcgaatgcgc tcggcggaaa gctggattgg
     9241 ggtcagcgga ttcttgatct cgtgagcgat gcgccgcgcg acgtcggccc aagccgtgct
     9301 ccgctgcgca ctgacgagtt cggtcatgtc gtcgaaggtc acgacaaagc cttgcacgtc
     9361 ctggtcctgg ccttcgctgg tgacgcgcac ggcgaaattg cgctccgaac cttgacgccg
     9421 cagcgtgacc tgatcggtga cgagacgttt tccctgcttc tgcgcccgct tgagcacctg
     9481 ggcgaactcc ggcacggcct tggcaagcgg ctgaccgacg agaacggcct ccttcatctc
     9541 cagcagcgtc tcggccgagc gattgaccaa ggtgacgacg ccgtggctgt cgacgccgac
     9601 aacgccggcg gtcacgcctg acaacacagc ctccatgaac tggcggcgct catcgagctt
     9661 cgaattggcg ccgatcagct cgtcacgctg actcttgagc tccgcggtca tggtgttgaa
     9721 ggtcgagcca agcaccgcga gatcgccatc ggcggggcga acatcgacat tgacgccgag
     9781 gttgccgcgc gaaatctgct cggccgcgcc catgagctgg cggatcgggg cgaccaggcg
     9841 gttggcgaac cacatgccga tccagatcgc cgaggcgagc agcgttaacg cgagcgccag
     9901 atagatgagg ccgtaagcca tctggaagtt ggtgcgccgc tgttcgagcc cctggtactc
     9961 ggcgacattg gcacgcgtgg cgctgaggtg ctgcagcacg cgcaggttca ccgggcgcat
    10021 cacgtagaga taggtgtcgg tgaagttgtt gaggctcttg atggcgccga ccatgttggt
    10081 gtctttgagt gagatcggga ccggcggacc tttcttggcg cgcgccataa cctcttccgg
    10141 gggcttcttg taggggagat ctcgaagttc tgccgctgtc gcaaggacct tcccctcgcc
    10201 gtcgatgagg tatgcgacgg gaaacccgtt catggcggcc tgggtgctga ggaaaccgcc
    10261 gaacttttgc ggcagggatc tagcgagccc cgcaccctcg tcgatatacc tcgccatagc
    10321 cacggtattg gcacggatca cctgctcatg ctcctccaga taggcctcgg cgacctccat
    10381 cgaattctgg acgatggctt gggttcggac ggagaaccaa tagtcgaggc cgcgaccgag
    10441 agagatgctg gcgaagatgg cgagcaggat cgctggcacg agcgcgatca cgctcaagag
    10501 gctgacgatg cggacgtgca gttgggcgcc ggcagcctgg cgctggcggg cgagccatag
    10561 cccggtgacc tgccaggcga tgatggcgat catcgccagg accggcacga gattgatgaa
    10621 aagcacgacc accacgacgt tgtcggtcgg cttgatgggc gtgagacctg tcaggatcac
    10681 ataagtggca agaccggagc acagcgaaag caccgcggtg atgaggccaa gcgtgaaggg
    10741 cagacggcgg cgcccttcgt cccatccggc cgcggttccg gccggtgcgg cgtctttggt
    10801 ccgggtggat ggatgggtca tcgtctcgcg cgaaccgcca agcgccgaac gctcggctcc
    10861 agcgtacatg gactgtaaga cgccacgaaa ggccatgccc ccgctctccc tcggacttgc
    10921 cgttgggagt tattgcctca gaaatgatgc aataatatca caatgtggcg ctttggcgac
    10981 aagtgatatt tcgagtcgcg atgggaccaa acctcgtgat cgcgaaggct cgataggaca
    11041 atccaaccac gatattgcct gcggaaagcc ggggcgtcac ccgccttgag accggatcac
    11101 cttgatgccg agatcgcgga tcttcttgcg cagagtgttg cggttaagcc ccaagagatc
    11161 ggcggcgcga atttggtttc cccgtgtcgc ggccagcgcg gccgtgagca gaggcagttc
    11221 gaccttgcgc aggatgtgtc cgtagaggcc gatcggcggc aggtccctgc cgaagctcat
    11281 gaaatactcg gcgagatagc gctcgaccgc ctcggagaga tcgcccgttg cctctgccgc
    11341 attggtaaac ggcgcgcggg ccgggctcgc caattcgttc tcgatcatgt cgacggtggc
    11401 gatgtcctgg gaatagatcg ccgaaagccg gcgcaccagg ttctcgagct cgcgaacgtt
    11461 gccgggccag tgatagcgct tcagccggtc gatcgccgcg ggctcgaagc ccttcggcgg
    11521 cagcccttca cgcgcggcga tggcgaggaa gtgacgaacg agatcgggaa tgtcctcgag
    11581 ccgttccctg agcggcggca agcggagcgg cacgacgttc aggcggtaga acagatcctc
    11641 gcggaacagg ccctgatcga tctgctgttg aagatcgcga ttggtcgcgg cgatgatccg
    11701 gacattggtc ttgatcggcg tgcggccgcc gaccgtggtg tattcgccct gctgcaacac
    11761 gcgcaagaga cgcgtctgcg cttccatcgg catgtcgccg atctcgtcga gaaagagcgt
    11821 gccgccttcg gcctgttcga agcgtccgat gtggcggttc tgggcgccgg tgaaagctcc
    11881 cttctcgtgg ccgaacagtt ccgactcgat cagctcgcgc gggatcgccg ccatgttgat
    11941 ggcaacgaag gatccgttgc ggcgcttgcc gtattcgtgc agcgcgcggg cgacgagttc
    12001 cttgccggtg cccgactccc ccgtgatcat gacggtcaga tcggtctgcg tgaggcgggc
    12061 gaggacgcgg tagatctcct gcattgccgc acagcgtccg atcagcggaa gaccttcggg
    12121 agcctcggcg gggagccgtt gcgcctttcg ccgcggctcg gcgagagcgc gccccaccac
    12181 ggcgacgagc tcgttgagat cgaagggctt gggcagatac tcataggcgc cgcgctccgc
    12241 cgccgtgatg gcggtcatga aggtgttctg cgcgctcatg acgatgatgg gcagatcggg
    12301 cctcaccttc ttgacgcggg gaatgagatc gaaggcattc tcgtcgggca tcagcacgtc
    12361 ggtgatgacg aggtcgcctt cgccctggct gatccaacgc cacagcgtcg cggcgttgcc
    12421 ggtcacccgc ggcgcgtaac cggcgcgcgc gagcgcctgg ttgagcacgg tgcggatcgc
    12481 ggcgtcgtcg tcggcgatga ggatgttgcc cttggtcatg cgatgctaac cctctacggt
    12541 gcgtggcggc gttgcgcctt ctgccattgg catcagcgcg cgaaacacgg tcagcctcgg
    12601 ccgcgagtcg cactcgatga tgccgccgtg atcgccgatg atcttggcca ccagcgcaag
    12661 accgagcccc gtgcccgacg gcttggtggt gacgaacgga tcgaacagat gcggcatcaa
    12721 gtcggcggag acgcccgcgc cgttgtcttc gaccgtgatc tcgagcggca ggctgacgcg
    12781 ggcttggctc cccggcaccg agaggcgcac gccgggccgg aacgaggtcg tgagcgtgat
    12841 ctggccgtcg gcgcgctcct cgccgatcgc ttcggcggcg ttcttgacga gattgagaaa
    12901 tgcctgaatg agcttgtcgc ggttccccgg caccggcggc agcgacggat catagttctc
    12961 gaggatcttc accttgcggg cgaagccggt ggcggcgatc cgtttcacgt gatcgagcac
    13021 gtcatgaatg ttgaccggct cgcgctgcaa cggccgctca tcaccgaaaa tctgcatgcg
    13081 atccacgagc ttgcagatgc gatcggactc cgcgcagatg agctgggtca aggtgcgatc
    13141 ggaatctacg cgctcgctct ccagcaattg cgccgctccc ctgatgccgg agagcgggtt
    13201 cttgatctca tgggcaagca cggcggcaag acccgaaacg gaacgcgcgg cgccgcgatg
    13261 ggtcagctgg cgctcgatca tctgcgccat gctgcgctgt tgcagcatca cgagcacgcg
    13321 gccggacaac tcggcgacag gcccgccgta aagatcgacc agcttcggcg cctcgaagcg
    13381 aaccgagccg agctcgacgc cgtattcatt gacggtcgcg ccgttccgct gcacctgctc
    13441 gacgagagcg atcagcggac tgccgaaagc gacgatttcg gtgagagcgc gcaggcacag
    13501 cacgcctgca ctcatctgaa agaaatcctc ggcggcggtg ttggcataat cgatggcgag
    13561 cttttcgccg atcacgagca gcggatgagg cagtgcgccg agaagtgcga tctcgatcgg
    13621 cggaaattcg accacgcgcc gcgcattgag ctttttggcc gagccgatca agcggtgtat
    13681 ccttggggcg caggcctcca cggccgcgcg tgcttgtgca taatttttat gcaagaaaat
    13741 gccatgacct ttccttgggc acgatattgg ccaatttctg ccgcggcaat aatcgttttg
    13801 ccgcagtgcg gcaacaaatt gatatttttt gtttttttct gtcttgtgag gacttcttcg
    13861 tgaccgtggc agccctgatc gtagctgcgg gccgggggtc ccgggcggcg ggcgacagag
    13921 ggcgcccgaa gcagtattgc ccaatcggcg gcgcgccgat gctcacccat actctcgccg
    13981 ccttcgccca tcatccacgc gtcgacgaca tcctcgtcgt gatccatccc gacgacgcgg
    14041 cgctctacgg ggaagcaagc gagccctatg cggcgcgact gcggtcgccg atcgcgggcg
    14101 gcgcgcggcg ccaggactcg gtgcgggccg gtctcgaggc gctcgcaaac gtcgcgccct
    14161 cctccgtcct catccacgac gcggcgcggc ccttcgtccc ggccgaactc atcgaccgcg
    14221 tcatcgacag ccttaaagtc catcaaggcg ccctccccgc tctgcctgtc accgacagct
    14281 tgaagcgcgc ggaagcgggc cgcgtgaccg gaacggtcgc gcgcgagagc gtgtggcagg
    14341 cgcagacgcc gcaagggttc gcgttcggcc ggatcctcgc ggcgcacctc gccgcggctg
    14401 aacgggagcc ggaccgcgac ttcagcgacg actccggcat tgccgagtgg ttcggcctcg
    14461 atgtcgcctt ggtcgaaggt gcggagagca accgcaagct caccacggcc gaggatcttc
    14521 gcatcgccga cgaactgctg cggccgaagc gcgcaaagga catgggcacc atacgcgtcg
    14581 cctcaggcta cgatgtccat gccctaggcc ccggcaatgc ggtcacgctg tgcggcgtga
    14641 gcattcccaa tcccaagtcg ctcgtcggcc acagcgatgc cgatgtcgga ttgcacgcgc
    14701 tgaccgacgc tctcctcggc accattgccg agggcgatat cggggtacac ttcccgcctt
    14761 ccgatgcccg ctggcgcggc gccgcatcgg agctgttcct caagcacgcc gcagcgatga
    14821 tcgtggagcg tggcggaacg atcgtgcatg tcgacgtcac cttgatctgc gagggcccgc
    14881 gcatcgggcc ctaccgcgac cggatgcgcg agcgcatcgc cggcatgctc ggtctcgacg
    14941 tggcgcaagt gagcatcaag gccaccacga atgaagggct cggctcgatc ggcaggggcg
    15001 aaggcatcgc ggccattgcg acggctacgg tcagcctgcc atgacggatc gcgtggaaat
    15061 cgacgcgaag ctcctcgccc ggacggcgaa actgctcgac gcgtgccgag agcggaacga
    15121 aacggtgacc gccgccgagt cctgcaccgg cgggctcgtc gcggcgacgc tcaccgccat
    15181 ccccggctct tccgatgtgt tcgagcgcgg cttcgtcacc tattcgaaca gcgccaaaag
    15241 cgagttgctc ggcgtgcctt actggctgat cgagcgccac ggcgccgtga gcgaggacgt
    15301 ggcgcgggcc atggctggcg gcgcgcttct ccattcgcat gcgagccttg ccgtggcggt
    15361 caccggaatt gccggaccga atggcgggac ggaagagaag ccggtcggtt tcgtttgctt
    15421 cgccgccgga aggcgggatg cgccgatcca gagcgaatgc gtgagcttcg gcgatctcgg
    15481 caggacggaa atcaggcggc ggagcgtcga gcgggcgatc gatctcctgc tgtcactgtt
    15541 gtgaagcgag cgcggccgcg tttcgcccat agacggcgtc ggccctcgcc tcgaatgcgc
    15601 gcgtgtatcg ttccaccgcc ttggcgaaga gccgcccgac caggcgctcg aacaggcgcg
    15661 agctgaaggc gtaggcgatg tagaacgcga cgtcgcagga atggctgccg cgcggctgaa
    15721 agtgccagcg attttccaga aatgtgaagg gaccgtcgag atactcgacc agaatcttgc
    15781 gcgccggcgg gtcgaggtga actttggtgg tgaagctttc ccggatcagg ccgtaaccga
    15841 ccgtcatttt ggcgacgatc acctctttgc cgtcgctcac gtcgcgtcgc ttgatttcga
    15901 gcgcctcgca gagcggcagg aatctcggat agtcctcgac cctcgcaacc agcgcgaaca
    15961 tctcatcggc cgaatgcgcc acggggtggg tggtctcgta gacatgcatg acgtttcccg
    16021 ccggtgcgcc gtttcaggcg ccgcgcgcgg cgcggcgcgc cgccttgagc cgggcgaagt
    16081 cttgcggcgc gtgataggac gagcgggtca aggggctcga cgccacgagc aggaatcctt
    16141 tgtcctcggc gatccgctta taagacttga actcctccgg cgtgacgaag cggtcaatgg
    16201 ccgcatgctt gcgggtcggc tggagatatt gcccgatggt gaggaaatcg acagcagcgg
    16261 cgcggaggtc gtccatcacc gcgacgactt ctccttccgt ctcgcccaag cccaccatga
    16321 tgcccgattt ggtgaagagg tcggggcgga gcgacttggc acgggacaag agccgcagcg
    16381 aatggcggta gtcggcgccg gggcgaatgc gccggtagag cgagggcacc gtctcgacat
    16441 tgtggttgaa cacgtcgggc acggcatcga gcacgatctc gagcgcgccg tccttgcgcc
    16501 ggaagtcggg ggtcaaaacc tcgacgctgg cagcggggca cgccgccctg atggcgcgga
    16561 tggtggcggc gaagtgcccc gccccgccgt cggcaagatc gtcgcgatcg accgaggtca
    16621 cgacgacatg ggcgagatcg agcgcggcaa cggcctgcgc cacccgcgcg ggctcctcgg
    16681 aatccagcgc gccgggcctg ccggtccgca cattgcagaa ggcgcaagcc cgcgtgcagg
    16741 tgtcgcccag gatcatgaag gtcgcgtggc gcttgcccca gcactcgccg atattgggac
    16801 aggacgcctc ctcgcacacc gtggtgagac gatggtcgcg caggatcgcg cgcgtcgcct
    16861 cgaaggcacg cgcccctgga gcccgcacgc ggatccagtc cggttttctc agaaccggcg
    16921 tatcgggcag ccgcgccttc tccggatggc gcggccgtgg cgttacccga tccaaacgct
    16981 caatcacggt caccatggag ccactcaagc cgccttcggt ctagaccgac gggcctctga
    17041 tggcacgcca gatccacaag atgaggatgg cgccgatcga ggccaccagc aaattaccga
    17101 gccagccgta gaattccacg ttgagaacgg aggccaaatt gcccccgacg aaagacccgg
    17161 cgatgccgac gagcagattg gtcaggaggc cgtggtcgct tgaggtgacc ttctcggcga
    17221 tatagccggc gatgatgccg atgatcagca tgccaataaa tcccacgccc ggcatagcga
    17281 gcaaggtttg cgcgttatcc attgtcgacc tcctcgaagt cttcgcgccg ctgcgtcgtc
    17341 agcgtgcgcg ggagccaaaa tccaaatgcc gtcgtcggcc gcctagatat ggagcgcgcg
    17401 gccgtaagcg tcgagtaccg attcgcctat catctccgac aatgtggggt gggggaagat
    17461 ggccgccatc aattcggcct cggttgtctc cagggttttg gcgatgacca ggctgtggat
    17521 cagctcggtg gcttctgcgc caatgatatg agccccgagc agcgcgccgg tcctggcgtc
    17581 gaagatagtc ttgaccaggc cttcggtttc gccgagggcg atcgccttgc cgttggccga
    17641 ataggggaag cggccgatct tcaagtcgcg gcctgccgcc cgcgccgcct cctcggtcaa
    17701 gccgacgctt gccacttgcg gggcgctgta ggtgcagctc gggatggcgt tccgatccaa
    17761 aagatgcacg ccctcgacgc ccgcgatctt ctcgacgcag agcacgccct cgtggcttgc
    17821 cttatgcgcg agccaggggg gtcctgccac gtcgccgatc gcatagatgc cggaagctgc
    17881 cgtgcgatac cattcatcga cgacgatgtg acctttctcg accttgactc cctgctgttc
    17941 aagcccaagc ccctcgacat tgccggtgat gccgattgcg aggatgacgc ggctcgcgcg
    18001 tagctcctcg gaattgccgt cgggctttcg tagctgcgct gtcacggcgt cggcgccctt
    18061 gaccaggcgc tcgacgcggg tgtcggtgcg aatggcgatg ccctgccgcg cgaaggcttt
    18121 gcgcgcgagc cccgaaatct cggcatcctc cgccgggaga atgcggggca gcatctcgac
    18181 cacggttacc tcggcgccga ggctccgata gaagctcgcg aactcgatgc cgatcgcgcc
    18241 cgagccgatg acgaggagcg attgcgggaa gctctgagga accattgcct cccggtaggt
    18301 ccagatcaat cggccgtggg gctccagtcc cggcaaggtt cgcgcgcgcg ctcccgtcgc
    18361 gacgatgata tgcgcggctt cgatatcggg cagcgtctcg cccgtcttgc ccgtgacggc
    18421 gatacgcccc tgccccatca gccgcgccgt cccgtcgatc acagcaatgt tgttcttgcg
    18481 catgagatag gcgacgccgt tcgacagccg ctccgccgct tttcggctcc gctcgaccac
    18541 gcgggcaaga tcgaagcgga cattgtcggt ggcgagcccg aagctcgaag cgtgccgcat
    18601 cgcctgatag agctcggagg tgcggagcag agcctttgtg gggatgcagc cccagttgag
    18661 gcaaatgccg ccgagatgct cgcgctcgac aatcgccgtc ttcagcccga gctggctcgc
    18721 gcggatggcg gcgacataac cgccgggtcc ggcgccgatg atagcgagat cgaaacgcgt
    18781 cgcggtcatt gacctctcgc ttctcccgtc aggcgccggt tcgcaagcga ctttaccatc
    18841 aacacccagt caccttctcg gtgagatcca ggaaacgcga tggcgggttc gctcgcaacc
    18901 gtggcatatc cggcgcggcg atagagacgc agagcggtcg cgttccaact tgcgacaatt
    18961 acgctcaaag caggtgcacc cgtctcgctt ccggtgcgtt cggcgatctc gagcagcttt
    19021 gccccgatgc cccgcccgcg aaactccggg aaggtcgcga gcacgttcac ataccaggag
    19081 cccggcgccg tcgcctccag cttgaccagg ggttgaacga tttccggcat atcggcaagc
    19141 gcgccgagat cgtagggatc gtcgaggcga tagccgacga ggctcgcagc gacctcgccg
    19201 ttcacctcgg cgaccacggc gttgcgccat gagaaaccgc cgctgtcgcg ctgggcgcgc
    19261 tcgcggccga attcgagcac cgactggcct ggcgcggcga gcgtgctcca catgtaagcg
    19321 ggcatgccct cgccggcgat gtcgacgagc accgcaagcg cggaagcgtc ggccttggtg
    19381 gcgggtctga acagggccgc agggtccatg cgaacggaga ctacaccagc atggtgacgg
    19441 ggtcttcgat atgggccttg aaggcggcga gcagctcggc gccgagcgcg ccgtcgatga
    19501 cgcggtgatc gaccgagagc gtcacgctca tcatggtcgc gatcttgagc gcgtcgtcct
    19561 tgaccacggc gcgcttctcg ccgcggccga ccgccaggat cgaggcttgc ggcggattga
    19621 tgacggcatc gaagcggtcg atgccgtaca tgccgagatt ggagatggtg gtactgcccc
    19681 cctgatattc gtgcggcgca agccgcttgg agcgcgcgcg ggcggcgaga tcgcgcatct
    19741 cgttcgagat ctccgacagg ctcttgagct cggcgtcgcg gatcacgggc gtatgcaggc
    19801 cgccgccttc gagcgcgacc gcaaccgcga tgtcggacgc gcgatggcgc aagagcccct
    19861 gctcggtcca gctcgcgttc gcggcaggca ctccctggag cgccatggcc atggccttga
    19921 tgatgaagtc gttgaccgag agcttgaagg cgcgcggtcc ctgttgcggc gcgagggcgt
    19981 tgagccgcgc gcgggcggcg agcagcgcgt c
//
LOCUS       DASUQP010000183        21498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1453422, whole genome shotgun sequence.
ACCESSION   DASUQP010000183 DASUQP010000000
VERSION     DASUQP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 21498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21498
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453422"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..2703)
                     /locus_tag="VFY74_08120"
     CDS             complement(40..2703)
                     /locus_tag="VFY74_08120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5931695.1"
                     /translation="MRNVLVAVVVAVATAMQASMWVLLHEQVLPPNANGALASLSFSP
                     VNPRHDGELDKTAEAQIRSDLAAIAPYTRTVRTYSVGNGLDLVPELASQYGLRATLGI
                     WINEFEHQNEEEIERAIELAKQHRNIDSIIVGNETIYRNKAIYKRENVEDLIAKIQRV
                     KRSVRVPVTTAEVWDIWLEHPELVSAVDYLAVHILPYWEGLPGSAAVDHAIKVYERLR
                     QAYPGKRIVIAEFGWPSAGLNRKDAVPSPITQAEVVRDFVARADAMGIDYSIIEAFDQ
                     PWKTGEGSVGPYWGIFNADREPKFALDGTVETPNFILKMMAAAGIGLLLSIPIFAIRG
                     VTVAQAGLLALAAQAIAAWSANVVDYWATHYLVLGSQVAMFAGGLLLIPLIIIIRRRI
                     EELAAILFGRKPRSLLGRVGAPPEQAAPFVSIHIPAYREPPEMLRQTLDSVATLDWPN
                     FECVVVINNTPDPALWGPIEDHCRLLGQRFKFVRADRLEGFKAGALRLALEHTAPEAE
                     IIGVLDADYVVHPDWLKDLVPAFADPAVGLVQAPQDHRDASRTVTQVAMNREYAGFFD
                     IGMVERNEANAIVTHGTMCLIRRQALREAGSWSSDTIVEDTDLGLSLLAQGWRAHYTQ
                     TRYGWGLLPCDFAAYKRQRHRWAYGGVQLIKKHWRAFLPGRSRLTPQQRSEYLFGWLT
                     WLGAEALGVLIAVLNLIWMPLVAFFGIAVPEAVLTLPVIATFAVMLLHFMVLYQKRVQ
                     APVFASLGAALSAMALQFTVGKAVADGLIKDGLPFVRTAKGGGSRWSRSFPALFEGVL
                     GVLLLIGALMLYLTNEQGVHEIAIFSAVLLVQSLPFLAAVGLALLERSPFNAFATWRR
                     LTAPSTYLPRLPRGPAPTSGSPEGEGASIMS"
     gene            complement(2790..3323)
                     /locus_tag="VFY74_08125"
     CDS             complement(2790..3323)
                     /locus_tag="VFY74_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931696.1"
                     /translation="MLRCVNFTASALVATAFLVSSATGFAQPPSGEQKPPEQAQQGEQ
                     KPEGEQKAAEQAKAEAEAAARATAEYEEAAAKLPHSAGAPECVWTGRRIASLLWRDDI
                     DTARRYIDLYDRFKCSSDHLKLVFRCVIKQGPIDPKASDHLASRVHNCWITPVGETTA
                     SSEKTNSPNTKAATIPN"
     gene            3494..5098
                     /locus_tag="VFY74_08130"
     CDS             3494..5098
                     /locus_tag="VFY74_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-glucan synthase"
                     /protein_id="HEX5931697.1"
                     /translation="MLRSLGLFALVAAAIAFAWHGLGRPIPMPASPLISGGKLTCISY
                     APFHGDQAPFTWNLVIPDRQIEGDLRRLSTVTPCVRTYSAKGPQGRVTRLAEAAGLEV
                     LQGIWLGRNREDNRREIEAALQMARLHPDTVKALIVGSETLLRGELPQKQIKAYLEEV
                     SRRSGLPVTYADVWEFWLKAPELAEATDFVTIHILPYWEDDPVREEEAVAHVRQVRQR
                     VAATFPGKEIVIGEVGWPSRGRMREGALPSPVNQARFVSGVVQLAQEEGWKVNLVEAF
                     DQPWKRLMEGTVGGYWGLYDGAGREPKFRFGEAVSNHPDWRLKAGLGIGAALIVFLAF
                     WSASRGLRAEGSWKHEAGATAIALSAGLVFGLAAVNLPLEGETSEDRLRAAGMVVLAL
                     VVPLAAAFALAKGSRLAGFAIALDPSRWRWSACIELVLAALLAATVVAAIHVALGLVF
                     DPRYKDFPFAALAPPVVALSALAFANRNQAAAPGTAEMVAAVLLAGSALFVVINEGLA
                     NWQALTFAGLLLALAFTCLKAEAVRE"
     gene            complement(5064..5468)
                     /locus_tag="VFY74_08135"
     CDS             complement(5064..5468)
                     /locus_tag="VFY74_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931698.1"
                     /translation="MSDAAPDTAPPKLFVPSRRAAAIIAILGLAALGAALFLRYAIIQ
                     NSVIGVACEAGEESLTCSVRLAVILLFVRGIFGWGALIAASIAFVRPNVVAFGAGVVL
                     AILGLVLYNTRSSALAVALLVLTLARPRPSGR"
     gene            5623..8277
                     /gene="pepN"
                     /locus_tag="VFY74_08140"
     CDS             5623..8277
                     /gene="pepN"
                     /locus_tag="VFY74_08140"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX5931699.1"
                     /translation="MLEPARRPKFLADYAPPDYLIEAVELDVALDPKDARVAAKLRVK
                     PNPKAATRGNPLMLDGEGLVLRSLALNGKRLAPEDYAVTETALTIPSVPAEPSTLDIV
                     TTCDPDANTALSGLYRSRGTYCTQCEPEGFRRITYFIDRPDVLAVYTVRIEAERAAAP
                     VLLSNGNPLAHGDIPGTGRHFAIWHDPFPKPSYLFALVGGDLARVADSFVTASGRKVD
                     LAIYVEPGKEDRCAWAMDSLKRAMRWDEERFGREYDLDVFNIVAVSDFNMGAMENKGL
                     NIFNDKLVLARPDTASDADYASIEGVIAHEYFHNWTGDRVTCRDWFQLCLKEGLTVFR
                     DQEFTADIRSGPVQRIAAVRMLKTHQFPEDAGPLAHPVRPASYIEINNFYTATVYEKG
                     AELCRMLQTLLGRDGFRKGLDLYFERHDGQAVTVEDFVAAMADASGRDLSQFMLWYTQ
                     SGTPELACALDYDAHAKTAWLSVSQVVPPTPGQAKKEPMLIPLKLGLIGANGDELPLK
                     LDGAPLGDGLIELTAREQIFTLRDVPSPPTPSLLRGFSAPVRITTGIDADRIEFLMTH
                     DSDPFNRWQAAQTYATNLLTAAARDGNGSELVRGEGAARLAQVLGATANDAALLPAYR
                     AEFLKLPGESDIARELARNVDTDAVRRIRESLRAIVGNAIGEILADLYENSAPNGPYS
                     PDAASAGLRALRSATLDLLVATGAPSEIARAERHYREATNMTDAIAALSILSHVPGPE
                     RDAALDHFYARWQEEPLVLDKWFGVQARAPRPNSVETVQALLSHPKFSLKNPNRIRAL
                     IGSFIHSNPTGFNRADGAGYRLLAGQALEIDRFNPHMAARLLGAFESWRILEQGRRAH
                     AKAALEGLAARSLSTDSYEIVSKTLGTA"
     gene            8427..10700
                     /locus_tag="VFY74_08145"
     CDS             8427..10700
                     /locus_tag="VFY74_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5931700.1"
                     /translation="MARARSAREGFAFAGPQWQDLRIAVGRYLPDAERLHRLVPLLLV
                     GFAAVAALGFSVELIGGKRAAFETAEHRLELIADVAASNLKGKSLKPGSDWQKALADS
                     LPKGALGAGRFILLADAEGKIRARAPLEAPSGDLLTVLGPQQPLTTFGADAGVLRLAL
                     FDGTDALVTVRNVRDAQLAFIQPVGAALADWRQGARLEITLLVLTGLVLALLAGGLWY
                     LAPASARAESGTLASELAEALPGCGVWRWNLARGRVHWSAPMYTLLGLDPNDKPMSFG
                     VVARALHPEDDLRGEIDRHLRDGLSLFDRSFRLRHAAGHWVTLRLRGQIARGQHDREP
                     YLTGIAVVADHAVPIGSADENARLRDAVETISEAFVLWDRENRLVMCNSKYQQFHGLS
                     DDMVRPGSSYDEVIAGASEPIVSKRVSVAGSDDDDSRTYEAQIEDGRWLHINERRTRD
                     GGYVSVGTDITVLKQSQNRLADSEQQLRTSVAELKLSRRELEQQKQQLVDLAEKYALE
                     KNRAEAANRAKSEFLANISHELRTPLNAVIGFSEVMQQSLFGPLGHPKYQEYARDILA
                     SGNYLLEVINDILDMSKIEAGRMSLDVGSVDLADIVADCMKVVSQGAAERNIVLERHG
                     PQRLPLEADRRALKQVFLNLLSNAVKFTRDGGSVDVHLSRSRGYVKIAIRDTGIGIAE
                     ADIAKLGRPFEQVENQFSKSHQGSGLGLAISGALVELHGGTLRIMSREGQGTTVICTL
                     PMRPVIADEEPKLAASA"
     gene            complement(10715..13654)
                     /locus_tag="VFY74_08150"
     CDS             complement(10715..13654)
                     /locus_tag="VFY74_08150"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX5931701.1"
                     /translation="MGDQGSVAAFVERITEAPCVYDAARGADVLDSLNRAFRDDCQSA
                     PAAGLIAEAPKARDLLEATFSASPYLASLALRNPADLAACLMRGPETHVTEARSELAA
                     AIAQAQTAQEVSSLLRRFKRRMALLAGLADLGGVWPTEGTLHVLSATADAVLEQATAF
                     LFRRAREAGQLEPQTEASAPGYFIIAMGKLGASELNYSSDIDLIVFYDADRAGLKPEI
                     EPSTFFVRLTRDLVRLLQEHTADGYVFRTDLRLRPDPGATQIALSVEAGLTYYESFGQ
                     NWERAALIKARVAAGDIAAGEAFLHQLAPFIWRKYLDFAAVADIHAMKRRVHSFKGHG
                     AIAVEGHDIKLGRGGIRDIEFFAQTQQLIAGGRHPELRTRGTVETLERLAKGGWIAPD
                     AAKELNAAYLFLRRIENRLQMVEDQQTHVIPAERAELDRVARLSGFAETNEFGDALLA
                     RFKRVETHYEALFEKLPEVPTSAPSLVVGADAENPAALAALERLGFRNPQAAIAAVSA
                     WQSGRYAATRSERARERLTQFVPSLLDAFGRTAEPDLALVTFDRVIGEMPAGLQLFSL
                     LAANPSLLRLIADIMGTAPRLARIIGRRPLLLDAVLDPGFFGTVPTPARRKEIARAAL
                     AEARDYQEALDRARIVGREQAFLIGVRVISGTLSARQAGAAYADLAETLIEALAERVE
                     EELVQSHGRLPEGEAAIVAMGKLGGREMTAGSDLDLIVVYDYEGENAQSDGTKSLLGG
                     QYYTRFTQRLIAALSAETAEGSLYQVDMRLRPSGSQGPVATKLSSFTAYQKGSAWTWE
                     HLALTRARALSGPARLRRQIDETIRGVLAAPRDRAAVAGDVRTMRARIADEKGTTDIW
                     DLKQVRGGLIDLEFIAQFLQIVTAATHPEALDQNTEAALAKLAASGALAPGDAEILVP
                     AARLYHTLTQVLRLCLGKPFAADEAPRALKDLLARAAEMPDFATLEATLKDTLAAVHE
                     AFDRIVA"
     gene            complement(13633..15093)
                     /locus_tag="VFY74_08155"
     CDS             complement(13633..15093)
                     /locus_tag="VFY74_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5931702.1"
                     /translation="MTALTKLFRTTTFRLSLTYLALFSAAAILAIAYIYWNTTVLLSR
                     QLNQTIDAELKGLAEQYRAGGLEQLVRIVAERSQTPGNSLYLVADSQGHRLAGNLSAV
                     APQLWNSLGPVEFYYRRPAAGGVERRLAFANVFRLPSDYRLIVGRDIEDRLELARLVR
                     SAMLWGLGVMALFGIGGGYWVSRNLLARIDTLAATTRTIMAGELGGRLPVTGSGDELD
                     RLSESLNLMLARIEQLMGGLREVSDNIAHDLKTPLNRLRNRVEAALREPYGEPVYREA
                     LERTIEEADSLIKTFNALLSIARIEAGAGGENRERLDVSALLCDVAELYEPVAEERGL
                     VLKAEARGPVFVRADRQLLGQAIANLIDNAIKYGTAGAARKETGAKPDVEVTATEKGD
                     VVEIAVTDRGPGVPAPDRERVLDRFVRLEASRSEPGSGLGLSLVAAVARLHGGSLRLE
                     DNAPGLRVVLALPSGSEATANGVPAQEQAASATRAA"
     gene            complement(15077..15757)
                     /locus_tag="VFY74_08160"
     CDS             complement(15077..15757)
                     /locus_tag="VFY74_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5931703.1"
                     /translation="MRVLVIEDDQETASFLKRALKESGHVAEYAGDGQTGLELARANA
                     YDVLIVDRMLPKRDGLAVIGTLRAEEIRTPALILSALGEVDDRVKGLRAGGDDYLTKP
                     YAFSELLARIEALARRGAPEEANTRLVVGDLVLDRLTHRVTRAGEAIILQPREFRLLE
                     YLMKHAGQVVTRTMLLENVWDYHFDPQTNVIDVHISRLRSKIDKNFGDPLLHTVRGAG
                     YSIRDSAH"
     gene            complement(15850..17400)
                     /locus_tag="VFY74_08165"
     CDS             complement(15850..17400)
                     /locus_tag="VFY74_08165"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX5931704.1"
                     /translation="MRFDQSSGTESPGARKPTRRLTLLAAGALIATGALGLMLGNSVP
                     LAVAETGAQQTIETPYGRAPLSFADLVAKVSPAVVSINVKGDVKVADNEMEIPGLPDL
                     PEDNPLYDFFKQFRKGMPQAPSKPSPSLAQGSGFFITPDGFIVTNNHVVEDAEDITVT
                     LEGGAKYTAKLIGSDPRTDVALVKVDAGTKVFPYVEFSSKAPRVGDWVLAVGNPFGLG
                     GTVTAGIISAHNRDIGSGPYDYLQIDAAVNRGNSGGPSFDLDGKVVGMNTAIFSPSGG
                     NVGIAFAVPAALVKEVVSELQSHGTVDRGWLGVVIQNVTDDIADSIGMAEAKGAMVTK
                     VTEDGPAAKADLKAGDVIVEVNGDKIDDSRDLARTIAELNPDTDVNLAIVRYGEKREV
                     SMKLGTFPSQKKLAALEEDKPDTGQQMKDLGLSLAPAAKFPGAGSEGVVITEVDPKSD
                     AADKGLKPGDVILAVAGVNVSSPSDVAAGVKKAMDGAKAEKDTVKVLMQVKTGDQTRF
                     VALSLKKA"
     gene            complement(17589..18068)
                     /locus_tag="VFY74_08170"
     CDS             complement(17589..18068)
                     /locus_tag="VFY74_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX5931705.1"
                     /translation="MSWRSLFATVSLVLGIACLAAGPARAVQADEMLTDPALEARARD
                     ISTGLRCLVCQNQSIDDSDAPLAKDLRLLVRERLKAGDSDGEVVDFIVARYGEFVLLK
                     PRLSRETLLLWFATPALLAAALLLIFLAYRRRRAASEAPSPLNAREKRRLDRLLDEP"
     gene            complement(18075..20063)
                     /locus_tag="VFY74_08175"
     CDS             complement(18075..20063)
                     /locus_tag="VFY74_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX5931706.1"
                     /translation="MIAETGHYALILALAVAIVQTVLPLIGARMRDPQLAAIAVPAAR
                     MQFVLVAIAFAALTAAYVTSDFSVKNVWQNSHSAKPLLYKISGVWGNHEGSMVLWVLI
                     LALFGASVATYGANLPRELKANVLAVQGSIAAAFLLFIIVTSNPFTRLDPAPFDGEGL
                     NPILQDPALSFHPPFLYAGYVGFSMAFSFAVAALIDGRIDAAWARWVRPWTLAAWMCL
                     TLGIAMGSWWAYYELGWGGFWFWDPVENASFMPWLAGTALLHSALVMEKRDALKVWTI
                     LLAIITFSLSLMGTFLVRSGVLTSVHTFAVDPARGVFILAIMGVFIGGAFALFAWRAP
                     LLSQGGLFAPISREGALVFNNLLLTTACAAVFVGTLYPLALEAITGEKISVGAPFFNL
                     TFVPLMVPLLVAMPFGPLLAWKRGDMVAAAQRLMAAAVVALAASLLLLAIWWGRGPWL
                     APFGFGLGLWLVLGALTEIAYRVKLTEADSAEIWRRFANLPRAAYGSALAHAGLGLAV
                     IGIVATTAWRSEEILALKPGDSASIAGYTLTFKGVAARDGPNYRERVGVLAVTRNGRA
                     VTELLPSKRQFTVERTDTTEAGIHASWGGDLYAVLGDPLKDGAYSIRLYFNPLVRLIW
                     FGALVMFFGGAISLSDRRLRVGAPKRGPAKVAPVTAGE"
     gene            complement(20085..20564)
                     /gene="ccmE"
                     /locus_tag="VFY74_08180"
     CDS             complement(20085..20564)
                     /gene="ccmE"
                     /locus_tag="VFY74_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5931707.1"
                     /translation="MTRKQRRGVLIGTCLVVLAVAVGLVLFAMRDSIVFFYSPSEVAA
                     MGIAPGTRFRLGGLVETGSVVRGEGTTIRFTVTDRAKTLPVAYTGVLPDLFREGQGVV
                     AEGTLEPDGVFRADHVLAKHDENYMPPEVAKKLKAQGVWREGASAKPAEADVKAKVN"
     gene            complement(20561..>21498)
                     /locus_tag="VFY74_08185"
     CDS             complement(20561..>21498)
                     /locus_tag="VFY74_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX5931708.1"
                     /translation="ARGADRTKTSGGTAKAGILIVGVALPLLSLGLYLTYGSPRLPDQ
                     PLAARLEDPASDQNVEALVAKVEARLRQNPEEGEGWEAIAPVYLRWGRYIEAAEAYAR
                     AIRLLGPSAKRLSGEGQALVLANDGLVTPEARKSLEEALKHDATLIEPRILLAIAKEQ
                     DGQFKAAAEDWRGLLATAKGDEPWRAMVEKRMQAAEAHLAGTPPATEQAAQTPAGEEG
                     EKGPTSADIAAAQAMDPAQRQAMIERMVQGLATRLDQEGGSLPDWLRLVRAYTVLERR
                     DDAVKALGRAKSAFSGNSQALEELDRLAAELGLKS"
BASE COUNT         3729 a         7085 c         7015 g         3669 t
ORIGIN      
        1 aacgcgctcc gcctgcgcag catcgatctg gactaagcct caagacatga tgctggcgcc
       61 ctcaccctcg ggcgagccgg acgtcggcgc gggtccgcgc ggaagccggg gcagataggt
      121 cgagggagcg gtaagccgcc gccacgtggc gaaggcattg aagggcgagc gctccagtag
      181 cgccaacccg actgcggcaa ggaaaggcag gctctgcacc aagagcacgg cgctgaagat
      241 cgcgatctca tgcacgccct gctcgttggt gaggtaaagc atcagcgctc cgatgaggag
      301 aagcacgcca agcacgccct cgaacagggc cgggaaggat cgcgaccagc gcgagccgcc
      361 acccttcgcc gtgcgcacga acggcaatcc gtccttgatc aggccgtccg cgaccgcctt
      421 gccgacggtg aattgcagcg ccatggccga aagcgccgca ccgagcgagg cgaagaccgg
      481 cgcctgcacg cgcttctgat agagcaccat aaagtgcagc agcatgacgg cgaaggtcgc
      541 gatcaccggc agcgtcagca cggcttccgg cacggcgatg ccgaaaaagg cgacgagcgg
      601 catccagatc aggttgagaa cggcgatcag caccccgagc gcctctgcgc cgagccaggt
      661 cagccagccg aacagatatt cgctgcgctg ctgcggcgtg agtcggctgc ggccgggcag
      721 gaacgcgcgc caatgcttct tgatgagctg cacgccgcca taggcccagc gatgccgctg
      781 tcgcttatag gcggcgaaat cgcacggaag cagcccccag ccgtagcgcg tttgcgtgta
      841 gtgcgcgcgc catccctgcg cgagcagcga aagcccgaga tcggtatcct cgacgatggt
      901 gtcgctcgac cagcttcccg cttcgcgcaa ggcctggcgc cggatgaggc acatcgtgcc
      961 gtgcgtcacg atggcattgg cctcgttcct ctcgaccatg ccgatatcga agaagccggc
     1021 atactcgcgg ttcatcgcca cttgcgtgac ggtgcgggac gcgtcgcgat ggtcctgcgg
     1081 cgcctgcacg aggccgacgg ccggatcggc gaaggccgga acgaggtcct tgagccagtc
     1141 gggatgcacg acatagtccg cgtcgagcac gccgatgatc tccgcctcgg gtgcggtgtg
     1201 ctccagcgcg agccgaagcg cgcccgcctt gaatccttcg agccggtcgg cgcgaacgaa
     1261 tttgaagcgc tgaccgagca gccggcaatg atcctcgatc ggaccccata gcgcggggtc
     1321 cggcgtgttg ttgatcacca cgacgcattc gaaattgggc cagtcgagcg tggcgacgct
     1381 atcaagagtc tgcctcagca tctcgggcgg ctcgcgatag gcgggaatgt gaatagacac
     1441 gaacggggcg gcctgctccg gcggcgcgcc gacccgcccg agcaggctgc ggggcttgcg
     1501 cccgaagagg atggcggcga gttcctcgat gcgccgcctt atgatgatga tcagcgggat
     1561 gagcaggagg ccgccggcga acatcgccac ttgcgagccc agcacgagat agtgggtcgc
     1621 ccaatagtcg accacattcg ccgaccaggc tgcgatggct tgcgcggcga gcgcaagcag
     1681 gcccgcttgg gctaccgtca ccccccggat agcgaagatc gggatcgaca acagaagccc
     1741 gatgccggcc gcggccatca tcttcaagat gaagttcggc gtttcgacgg taccgtccaa
     1801 ggcgaatttc ggctcgcggt cggcgttgaa gatgccccaa tacgggccca cgctgccttc
     1861 gccggtcttc cacggctggt cgaaggcctc gatgatcgaa tagtcgatgc ccatcgcatc
     1921 cgcgcgcgcg acgaaatcgc gcacgacctc ggcctgcgtg atggggctcg gcacggcgtc
     1981 cttgcggttc agtcccgcgc tcggccagcc gaactcggcg atcacgatgc gcttgccggg
     2041 atacgcttgc cgcagccgct catagacctt gatggcgtga tcgacggcgg ccgagccggg
     2101 caggccttcc cagtagggca ggatgtggac ggcgagatag tcgacggccg agacgagctc
     2161 gggatgctcg agccagatat cccacacctc ggcggtggtg acggggacgc gcacgctccg
     2221 cttcacgcgc tggatcttgg cgatcagatc ctcgacgttc tcgcgcttgt agatggcctt
     2281 gttgcggtag atcgtctcgt tgccgacgat gatgctgtcg atattgcgat gctgtttcgc
     2341 aagttcgatt gcgcgctcga tctcctcctc gttttgatgc tcaaattcat tgatccagat
     2401 gccgagagtg gcgcgcaagc catattggga agccagttcc ggcacgagat cgaggccatt
     2461 gccgacgcta taggtcctca ccgtgcgcgt gtaaggcgcg atggccgcga ggtcggacct
     2521 gatttgggcc tctgccgtct tgtcgagctc gccgtcatgc ctcggattga cgggagagaa
     2581 cgacagcgat gccagcgccc cgttggcgtt cgggggcaat acctgctcgt gcagcaaaac
     2641 ccacattgaa gcttgcatcg cggtcgcgac ggcgaccaca acggcgacga ggacgttgcg
     2701 catctctgct ttgagtctcg ttggtgtccg tgaggcggca gactaggccg cagtactgga
     2761 acgctttcgc gcccaaatgg ttgcggctct taatttggta tcgttgcggc tttcgtgttt
     2821 gggctgttgg ttttttcgct cgaggccgtg gtctcgccca ccggtgtgat ccagcagttg
     2881 tgcacgcggg atgcgaggtg gtcggacgcc tttggatcga ttgggccctg tttgatcaca
     2941 cagcgaaaaa caagcttcaa atgatccgat gagcatttga agcggtcgta gagatcgatg
     3001 tagcggcgcg ccgtgtcgat gtcgtcgcgc cacagcaggc tcgcgatgcg ccggcccgtc
     3061 caaacgcatt cgggcgcgcc agcggaatgc ggcagcttcg ccgccgcctc ctcgtattcg
     3121 gcggtcgcgc gggcagccgc ctccgcttcg gcctttgcct gttctgccgc cttctgctcg
     3181 ccttcgggct tctgctcgcc ctgctgcgcc tgttcgggcg gcttctgttc gccgctcggc
     3241 ggctgggcga agccggtggc cgaagagacg agaaaggccg tcgcgacgag ggcggaagcg
     3301 gtgaaattaa cgcagcgcag catggagcac agcccgcaat aacgagtcat tttttaccac
     3361 cgagaccagg agattttgac agcccgaggg caagcgactc cgtatcattt cgccgacgcg
     3421 caaacgagaa aatttggttc aggaccgacg cctcagctgc tatttgtcgc atccccgccc
     3481 gcgaagtctc agcatgctcc gctcccttgg cctgtttgcc ctcgtcgccg ccgccatcgc
     3541 cttcgcctgg catggtctcg gacggccgat cccgatgccg gcctcgccgc taatctccgg
     3601 cggcaaacta acgtgcatct cctatgcccc gttccatggc gaccaggctc cgttcacctg
     3661 gaatctggtg atcccggacc ggcagatcga gggggacttg cggcggctca gcacggtgac
     3721 accctgcgtc aggacctatt ccgcaaaggg tccccagggg cgcgtcacaa ggctcgcgga
     3781 ggcagccggc ctcgaagtcc tacaaggcat ctggctcggt cggaaccgcg aggacaatcg
     3841 ccgggagatc gaggcggcgc tccagatggc gcgcctgcac cccgatacgg tcaaggccct
     3901 gattgtcggg agcgagacct tgctcagggg cgagctcccc cagaagcaga tcaaggccta
     3961 tctcgaggag gtttcgaggc gttccggcct ccccgtcacc tatgccgatg tgtgggagtt
     4021 ctggctcaag gcgcccgagc ttgccgaggc caccgacttc gtgactatcc acatcctccc
     4081 ctactgggag gatgatccgg tgcgggagga agaggccgtt gcccatgtgc ggcaggtccg
     4141 ccaacgtgtc gccgcgacct ttcccggcaa ggagatcgtg atcggggagg tcggttggcc
     4201 aagccggggc cggatgcgcg agggggcgct cccctcgccc gtaaaccagg cacgcttcgt
     4261 gagcggcgtc gtccagctcg ctcaagaaga gggctggaaa gtcaacttgg tcgaggcttt
     4321 cgaccaaccc tggaagcggc tcatggaggg gactgtcggc ggctattggg ggctctatga
     4381 cggcgctggc cgcgagccaa agttccgctt cggcgaagcg gtgtcgaacc accccgactg
     4441 gcggctcaag gccgggctcg gcatcggggc ggccttaatc gtctttcttg ccttctggtc
     4501 ggcgtcgcgc ggtttgcggg ccgaaggctc atggaagcac gaggcaggcg caacggcgat
     4561 cgcgttgagc gcgggcctcg tcttcgggct cgccgcggtc aacttgccgc tcgaaggcga
     4621 gacctccgag gacaggttga gagcggccgg catggtcgtc cttgcccttg tcgtcccact
     4681 cgcggccgcc tttgccctgg cgaagggaag ccggctcgca ggcttcgcga tcgcgctcga
     4741 tccttctcgc tggcgatggt ccgcatgcat tgaactggtg ctggcagcgc ttctcgccgc
     4801 gacggtggtc gccgccattc acgtggcgct cgggcttgtc ttcgatcccc gctacaagga
     4861 ctttcctttt gccgcgcttg cgccgcctgt cgtcgcgctg tccgccctcg cctttgccaa
     4921 tcgtaaccag gctgcagcac cggggactgc cgagatggtc gcggcagttc tcttggcggg
     4981 ttcggcgctg tttgttgtca taaatgaggg ccttgccaat tggcaggcct tgaccttcgc
     5041 aggccttctg ttggctctcg ccttcacctg cctgaaggcc gaggccgtgc gagagtgagc
     5101 accagcagcg ccacggcaag cgccgaactg cgcgtgttgt agagcacaag cccgaggatc
     5161 gcgagaacga cccctgcccc gaaggccacg acgttggggc gaacgaacgc gatagaagcc
     5221 gcgatcaacg ccccccatcc gaaaatgccg cgcacgaaca gcaagatcac ggcaagcctg
     5281 accgagcagg tgaggctctc ttcgcccgcc tcgcaagcca cgccgatcac cgagttctga
     5341 atgatggcgt agcgcaggaa gagcgcggca ccgagcgcgg caagcccgag aatggcgatg
     5401 attgccgccg cccgccgcga cggcacgaac agctttggcg gggcggtatc cggggcggcg
     5461 tcgctcatgc tgggccgcgg ctttaagcag gtacggcccg tcgcgccaga ggaccgctgt
     5521 cgctcagcgc gcccgagcgc ctttgcaacc acacatttcc agcgtatttc aaccggtccg
     5581 cctgcgcttg aaagttccaa gcgcgacaac gaagggatgt ccatgctcga gcccgctcgt
     5641 aggcccaaat ttctcgcgga ctatgcgccg cccgactatc tcatcgaggc ggtcgaactc
     5701 gatgtcgcgc tcgatcctaa ggacgcgcgc gtcgccgcca agctcagagt taagcccaat
     5761 cccaaggcag cgacgcgcgg aaatccgctc atgctcgacg gcgaagggct tgtcctcagg
     5821 agcctcgcgt tgaacggcaa gcgtctggcg cccgaggact acgccgtcac cgagaccgcg
     5881 ctcaccatcc catccgtccc cgccgagccg tccacgctcg acatcgtgac cacctgcgat
     5941 cctgacgcga acaccgcgct ttccggcctc taccgctcgc gcggcacgta ttgcacccag
     6001 tgcgagccgg aggggttccg ccgcatcacc tatttcatcg acaggccgga cgtgctcgcc
     6061 gtctacaccg tccgtatcga ggccgaacgc gccgcggcgc ccgtgcttct gtcgaacggc
     6121 aatccgcttg cgcacggcga tatccccggc acggggcgcc atttcgccat ctggcacgat
     6181 cccttcccca agccgtctta tctctttgcg ctcgtcggcg gcgatctcgc ccgcgttgcc
     6241 gacagtttcg tcaccgcctc ggggcgcaag gtcgacctcg ccatctatgt cgagcccggc
     6301 aaggaggacc gctgcgcctg ggcgatggat tcgctgaagc gcgccatgcg ctgggacgag
     6361 gagcgtttcg gccgcgaata cgatctcgac gtgttcaaca tcgtcgccgt cagcgacttc
     6421 aacatgggcg cgatggagaa caagggcctc aacatcttca acgacaagct cgtgctcgcg
     6481 cgccccgaca ccgcgagcga cgccgactac gcctcgatcg aaggggtgat cgcgcacgag
     6541 tattttcaca actggacggg cgaccgcgtc acctgccgcg actggttcca gctctgtctc
     6601 aaggagggcc tgaccgtctt tcgtgatcag gaattcaccg ccgatatccg ctcgggcccc
     6661 gtgcagcgca tcgccgcggt gcgcatgctg aagacgcatc aatttcccga ggatgcggga
     6721 cccctcgcgc atcccgtgcg ccccgcctcc tatatcgaga tcaacaattt ctacacggcg
     6781 accgtctacg agaagggcgc cgaactctgc cgcatgttgc agaccctgct cggccgcgac
     6841 ggtttccgca aagggctcga tctctatttc gaacgccatg acggccaggc ggtgacggtc
     6901 gaggacttcg tcgcggcgat ggccgacgcg tccggccgcg atctctcgca attcatgctg
     6961 tggtacaccc aaagcgggac gcccgagctt gcctgcgcgc tcgactacga cgcgcatgcc
     7021 aagaccgcgt ggctttcggt gagccaggtc gttccgccca cgcccggcca agcgaagaag
     7081 gagccgatgc tgatcccgct caagctcggg ctgatcggcg ccaatggcga tgagcttccc
     7141 ttgaagctcg acggcgctcc cctcggcgac ggcctcatcg agttgaccgc ccgcgagcag
     7201 atcttcacct tacgcgatgt cccatcgccg ccgactccgt cgctgctgcg cggcttctcc
     7261 gcaccggttc gaatcaccac cggcatcgat gccgacagga tcgagttcct gatgacgcac
     7321 gacagcgacc cgttcaaccg ctggcaggcg gcgcagactt acgccaccaa ccttctcacc
     7381 gccgccgcgc gcgacggaaa cggctccgag ctggtgcgag gcgaaggggc cgcgcggctc
     7441 gcgcaggtcc tcggcgccac ggcaaacgat gcggcgctgc ttccggccta tcgcgccgag
     7501 ttcctcaagc ttccgggcga atcggatatc gcgcgcgagc ttgcgcgcaa tgtcgatacc
     7561 gatgccgtgc gccggatccg cgagtccttg cgcgcaatcg tcggcaacgc gatcggcgaa
     7621 atattggcag acctctacga gaactccgcg ccgaacgggc cctactcgcc ggacgccgcg
     7681 agcgcaggcc tcagagcgct caggagcgcc acgctcgatc tcctggtcgc caccggcgcg
     7741 ccttcggaga tcgcccgcgc cgaacgccat taccgcgagg cgaccaacat gaccgatgcc
     7801 atcgccgctc tctccattct gtcgcacgtg ccgggaccgg agcgggatgc cgcgctcgat
     7861 catttctatg cgcgctggca ggaggagcct ctggtgctcg acaagtggtt cggggtgcag
     7921 gcgcgggcgc cgcgcccgaa ttcggtcgag accgtgcagg cgctcctgtc ccaccccaaa
     7981 ttctcgctga agaaccccaa tcggattcgc gcgctgatcg gaagcttcat ccattccaac
     8041 ccgaccggct tcaaccgggc cgacggagcc ggctaccgcc ttctcgccgg tcaggcgctc
     8101 gagatcgacc gtttcaaccc gcacatggcc gcgcggcttc tcggtgcctt cgagagctgg
     8161 cgcatcctcg aacaaggacg ccgagcccac gccaaggcgg cgctcgaagg cttggcggcc
     8221 cggtctttat ccaccgacag ctacgagatc gtcagcaaaa ccttgggcac ggcctaagag
     8281 tcggcgaaac gtcaacgggt tattaaccga atttccacag ctttactctg gacagaatgc
     8341 ggcttcgtcg attccaatgg acgagggcga ttcggagatg cggcacgtct ccggctcgaa
     8401 gcaggagaac gtaaaagggg gaccgaatgg ctcgggcgcg ttcggcgcgt gagggattcg
     8461 cttttgcggg accgcaatgg caggacctcc gcatcgccgt cggcaggtac cttcccgacg
     8521 cggaacggct tcaccgcctg gtgcctctcc tgctcgtggg cttcgccgcc gttgccgctc
     8581 tcggtttctc cgtcgaactg atagggggca agcgcgccgc cttcgagacg gcggagcacc
     8641 gcctcgaact gatcgccgac gtcgccgcct ccaacctcaa gggcaagtcg ctcaagccgg
     8701 gaagcgattg gcaaaaggct ctggccgaca gccttcccaa gggtgcgctc ggggcgggcc
     8761 gcttcatcct gcttgccgac gccgaaggca agattcgcgc ccgcgcgccg ctcgaagcgc
     8821 cctcgggcga ccttctcacc gttctcgggc cccagcagcc tctcaccacc ttcggagcgg
     8881 atgcgggcgt gctgcgtctc gcactgttcg acgggaccga cgccctcgtc accgtacgca
     8941 acgtgcgtga cgcgcaactt gcgttcatcc agcccgtcgg cgccgccctt gccgattggc
     9001 ggcagggagc gcgcctcgag atcacgcttc tcgtgctcac cggccttgtg ctcgcgctcc
     9061 ttgccggggg gctgtggtat ctcgcgcctg cctcggcgcg ggcggagagc ggcacgcttg
     9121 cgagcgagct ggccgaggcg ttgcccggtt gcggcgtatg gcgctggaat ctcgcccgcg
     9181 gccgcgtgca ctggtcggcg ccgatgtaca cgctgcttgg cctcgacccg aacgacaagc
     9241 ccatgtcctt cggcgtcgtc gcccgggcgc tccaccccga ggacgatctg cgcggcgaga
     9301 tcgaccggca cctgcgcgac ggactctccc tcttcgaccg cagcttccgg ctccgccatg
     9361 ccgccggtca ttgggtgacc ttaaggctgc gcggacagat cgcgcgcggc cagcacgatc
     9421 gcgagcccta cctgaccggc atcgccgttg tcgccgacca cgccgtcccg atcggatcgg
     9481 ccgacgagaa tgcgcggctg cgcgacgcgg tggaaacgat ttccgaggcc ttcgtgctgt
     9541 gggaccggga aaatcgtctg gtgatgtgca acagcaagta ccagcagttt cacggcctct
     9601 ccgacgacat ggtccggccc ggcagctcct atgacgaagt gatcgccgga gcgtccgagc
     9661 cgatcgtctc caagcgcgtc agcgtggccg gttccgacga tgacgacagc cgcacctacg
     9721 aggctcagat cgaggacggc cgctggcttc acatcaacga gcgccgcacc cgtgacgggg
     9781 gctatgtcag cgtcggcacc gacatcaccg tcttgaagca gagccagaat cgtctcgccg
     9841 acagcgaaca gcagctccgc acgagtgtcg ccgaactcaa gctctcgcgc cgcgagcttg
     9901 agcagcagaa acagcagctt gtcgatctcg ccgagaaata cgcgctggag aagaacaggg
     9961 ccgaagccgc caatcgcgcc aaatccgagt tcctcgccaa tatcagccac gagctgcgca
    10021 cgccgctcaa cgccgtgatc ggcttctccg aagtgatgca gcagtcgttg ttcggccctc
    10081 tcggccatcc caagtaccag gaatacgcgc gcgacatcct ggcaagcggc aactatctgc
    10141 tcgaagtgat caacgacatt ctcgatatgt cgaagatcga ggccggccgc atgtcgctcg
    10201 atgtcggcag cgtcgatctc gccgatattg tcgccgattg catgaaggtg gtgagccagg
    10261 gcgcagccga gcgcaatatc gtcctcgagc gtcatgggcc gcagcgcctg ccgctggagg
    10321 ccgatcggcg cgcgctcaag caggtctttc tcaatttgct ctccaatgcg gtcaaattca
    10381 cccgcgacgg cggcagcgtc gacgtgcatt tgtcgcgttc gcgcggctac gtgaagatcg
    10441 cgatcaggga caccggcatc ggcatcgccg aagccgacat cgccaagctc ggccggccct
    10501 tcgagcaagt ggagaaccag ttctcgaaaa gccaccaggg aagcgggctc gggcttgcga
    10561 tctcgggcgc gctggtcgag ctgcacggcg ggacgctgcg catcatgagc cgcgaaggcc
    10621 agggcacgac cgtgatctgc acgctcccca tgcggcccgt gatcgccgac gaagagccga
    10681 aactcgccgc ctccgcttaa gccgcctcct ctccttacgc cacgatgcgg tcgaaagcct
    10741 cgtgcacggc ggcgagcgtg tccttcaacg tcgcttcgag cgtggcgaaa tccggcatct
    10801 cagcagcgcg ggcgagcaag tctttcagcg ctcgcggcgc ctcgtcggcc gcgaagggct
    10861 tgcccagaca aagcctcagc acctgcgtga gcgtgtgata gagccgcgcc gccggcacca
    10921 gaatctcggc atcgccgggc gcgagcgcgc cggacgcggc aagcttggca agcgcggcct
    10981 ccgtgttctg atcgagcgct tcggggtgcg tcgcggccgt tacgatttgc agaaactggg
    11041 cgatgaattc gaggtcgatc agtccgcccc gcacctgctt caggtcccag atatcggtgg
    11101 tgcccttctc gtcggcgatc cgtgcgcgca tggtcctgac atcgcctgcg accgcggccc
    11161 tgtcgcgcgg ggcggcgaga acccctctga tcgtttcgtc gatctgacgc ctgagccgcg
    11221 ctggaccgct caaggcgcgc gcccgcgtca gcgcgagatg ctcccaagtc caagccgaac
    11281 ccttctgata ggcggtgaag ctcgagagct tggtcgcgac cggcccttgg ctacccgagg
    11341 gtctcagtct catatcgacc tgataaagcg agccctcggc cgtctcggcc gagagcgcgg
    11401 cgatcaggcg ctgcgtgaag cgcgtatagt attgcccacc aagaaggctc ttggtcccgt
    11461 cggactgagc attctcgcct tcgtagtcat agacgacgat gagatcgagg tcggaccccg
    11521 cggtcatctc cctgccccca agcttgccca tggcaacgat ggccgcttcg ccttccggca
    11581 ggcgtccatg gctctggacg agttcctcct cgacccgctc ggccagcgcc tcgatcaagg
    11641 tctcggcgag atcggcgtaa gcggcgccgg cttgccgcgc agagagcgtg ccggagatca
    11701 cccgcacgcc gatgagaaag gcttgttcgc ggccgacgat ccgcgcccgg tcgagcgcct
    11761 cctgatagtc gcgggcctcg gcgagcgcgg cgcgggcaat ctccttccgc cttgccgggg
    11821 tgggcacggt gccgaaaaag ccggggtcga gcacggcgtc gaggagaagc ggccgtcggc
    11881 cgatgatgcg ggcaagcctc ggcgccgtcc ccatgatgtc ggcaattagt cggagcaggc
    11941 ttggattggc ggcgagcaac gagaagagct ggagcccggc cggcatctcg ccgatcaccc
    12001 ggtcgaaggt gacaagcgca agatcaggct cggcggtacg gccgaacgca tccagaagcg
    12061 agggaacgaa ttgggttagc cgctcccggg cgcgctcgct gcgggttgcg gcatagcggc
    12121 ccgactgcca ggcgctgaca gcggcgattg ccgcttgcgg gttgcggaag ccgaggcgct
    12181 ccagtgcggc aagggccgcc ggattttccg cgtcggcgcc gacgacaagg ctcggggctg
    12241 aggtcgggac ctccggcagt ttctcgaaca acgcctcgta atgggtctcg acccgcttga
    12301 aacgggcaag cagcgcatct ccgaattcat tggtctcggc aaagcccgac agtcgggcga
    12361 cgcgatcgag ttccgcccgt tcggccggaa tgacgtgggt ctgctggtct tccaccattt
    12421 gcagccggtt ctcgatgcgg cgcagaaaga gataggcggc attgagttcc ttcgcggcat
    12481 ccggcgcgat ccagccgcct ttggctagac gctcgagcgt ctcgacggtc cccctggtcc
    12541 tgagctcggg atgacggccg ccggcgatga gctgctgcgt ctgggcgaag aactcgatgt
    12601 cgcggatgcc gccgcgcccg agcttgatgt cgtgcccttc cacggcgatc gcgccatggc
    12661 ccttgaagct gtgcacgcgc cgcttcatgg cgtggatgtc ggcgacggcg gcgaagtcga
    12721 gatatttccg ccagatgaag ggcgcgagct gatgcaggaa cgcctcgccc gcggcgatat
    12781 cgccggcggc gacccgcgcc ttgatcaggg ccgcgcgctc ccagttctgg ccgaagctct
    12841 cgtaataggt cagccccgcc tcgaccgaga gagcaatctg tgtcgcgccg gggtcgggcc
    12901 tcaatctcag atcggtgcgg aacacgtagc cgtcggccgt gtgctcctgg agcagccgga
    12961 cgaggtcgcg cgtgagccgc acgaagaagg tcgacggctc gatttcgggc ttaagccccg
    13021 cgcggtcggc gtcatagaag acgataaggt cgatatcgct cgaatagttg agctcggagg
    13081 cgccgagctt gcccatggcg atgatgaaat agccgggcgc ggaagcctcc gtttgcggct
    13141 ccaattgccc cgcctcgcgc gccctgcgga acaggaacgc ggtcgcctgc tccaacaccg
    13201 catcggccgt ggcgctcagc acatgaagcg tcccctcggt cggccagacg ccgccgagat
    13261 cggcgaggcc ggcgagcaaa gccatgcgcc gcttgaagcg ccgcagaagc gatgagacct
    13321 cctgggcggt ctgagcttgg gcgatagcgg ccgcaagctc cgaacgtgct tcggtgacat
    13381 gcgtctcggg accccgcatc aggcatgcgg cgagatcggc gggattcctg agcgcgaggg
    13441 aggcaaggta gggcgaagcg gaaaacgtcg cttctagaag atcgcgcgct ttcggagcct
    13501 cggcgatgag ccccgcggcc ggggcagact gacagtcgtc acggaacgcc cggttaaggc
    13561 tatcgagaac gtcggctccc cgcgccgcat cgtacacgca tggggcctcg gtgatgcgct
    13621 cgacgaacgc cgctacgctg ccctggtcgc cgatgccgcc tgctcctgag ccggtacccc
    13681 gttcgcagtc gcctcgctcc cgctcggcaa agcgaggacc actctgagcc cgggcgcgtt
    13741 gtcctccagt ctcagactgc caccatgcaa ccgggccacc gccgcgacaa ggctcaatcc
    13801 aagtccgctg cccggttcgg accggctcgc ttcgagccgg acgaaacggt cgagcacgcg
    13861 ctcccgatca ggagccggaa cgccgggccc gcgatcggtc accgcaatct cgacgacatc
    13921 gcctttttcc gttgcggtga cctccacgtc tggcttcgcg cccgtttcct tccgagccgc
    13981 cccggccgtt ccatatttga ttgcattgtc gatgagattg gcgatcgcct gcccgagcag
    14041 ctgccggtcg gcgcggacga agaccggccc gcgcgcctcg gccttgagca cgagcccgcg
    14101 ctcctcggcg accggctcgt agagctccgc cacgtcgcaa aggagtgccg agacgtcgag
    14161 cctttcgcgg ttctcgccgc cggcgccggc ctcgatgcgg gcgatcgaaa ggagcgcgtt
    14221 gaaggtcttg atcagactat cggcctcctc gatggttcgc tcgagcgcct cgcggtagac
    14281 cggctcgccg taaggttcgc gcagcgcggc ttcgacgcgg ttgcgcaggc ggttgagcgg
    14341 cgtcttgagg tcgtgcgcga tattgtccga gacctcgcgc aagcccccca tgagctgctc
    14401 gatccgcgcg agcatgagat tgaggctttc cgacaagcgg tcgagctcgt cgccggagcc
    14461 cgtgacgggc aggcgtccgc cgagttcacc ggccatgatg gtgcgcgtcg tggcggcaag
    14521 cgtgtcgatg cgggcgagca gattgcggct cacccaatag ccgcccccga tgccgaagag
    14581 tgccatcacg ccgagccccc acagcatggc cgaacggacc aggcgtgcaa gttcgaggcg
    14641 atcttcgata tcgcgcccga cgatgaggcg gtaatcgctc gggagccgga acacattggc
    14701 gaaagcgagc cgtcgttcga cgcctccggc cgctggacgc cggtaataga attccaccgg
    14761 accgagacta ttccacagct gcggcgccac cgcgctgaga tttcccgcca gccggtggcc
    14821 ttggctgtcg gccacaagat agaggctgtt gcccggcgtc tggctgcgtt cggccacgat
    14881 gcggacgagc tgttccagtc cccctgcccg gtactgctcg gcaagaccct tgagttcggc
    14941 gtcgatggtc tgattgagtt gccgcgagag caggaccgtg gtgttccagt agatataggc
    15001 gatggcgagg atcgctgcgg cgctgaacag cgcaagatag gtcagcgata gacgaaaggt
    15061 ggtggtccgg aagagcttag tgagcgctgt cacggatgct atagccggcg ccgcggaccg
    15121 tatgaaggag cgggtcgccg aaattcttgt cgatcttgga ccgaagccgg gaaatgtgaa
    15181 cgtcgatgac attggtctgc gggtcgaaat gatagtccca gacattttcg agcagcatcg
    15241 tgcgcgtcac gacctggccc gcgtgcttca tgagatattc gagcaaacga aactcgcgcg
    15301 gctgcaagat aatggcctcg cccgcgcgcg tcaccctatg cgtgaggcgg tcaagcacca
    15361 ggtcgcccac caccaggcgc gtgttcgcct cctccggcgc gccgcgccgg gccaacgcct
    15421 cgatccgggc cagaagctcg ctgaaggcat agggcttggt caaatagtcg tcgccgccgg
    15481 cacgcaagcc tttgacgcgg tcgtcgacct cgccgagcgc gctcaagatg agagcaggcg
    15541 ttctgatctc ctcggcgcgc agcgtgccga tcaccgcaag cccgtcgcgt ttcggcagca
    15601 tgcgatcgac gatcagcacg tcgtaggcat tggctctggc gagttcgagc cctgtctgtc
    15661 catcgccggc gtattcggcc acatggccgg actctttcag ggcgcgtttg aggaaggaag
    15721 cggtctcctg atcgtcctcg ataaccagca cacgcatggt ttccgatctc cgggtgacgc
    15781 tgcctggcaa aaagggccgg gcggcgcggt ggggggaagc cgcgccgccc tccggcttcg
    15841 aaggcgcgtt taggccttct tcagggagag cgcaacgaag cgcgtctggt cgccggtctt
    15901 gacctgcatg aggaccttga ccgtgtcctt ttcggccttg gcgccatcca tcgccttctt
    15961 gacgcctgcg gcgacatcgg acgggctgga gacgttgaca ccggcaaccg cgaggatgac
    16021 gtcgccgggc ttaaggccct tgtccgcggc gtcgctcttc gggtccacct cggtgatcac
    16081 cacgccctcg ctgccggcgc cgggaaactt cgcggcgggc gccagcgaca ggccgagatc
    16141 cttcatctgc tggcccgtgt cgggcttgtc ctcttcgagc gcggcaagct tcttctggct
    16201 cggaaacgtg ccgagcttca tgctgacttc acgcttctcg ccgtagcgaa cgattgcgag
    16261 attgacgtcc gtgtcgggat tcaactcggc aatggtgcgg gcaaggtcgc gcgagtcgtc
    16321 gatcttgtcg ccgttcacct cgacgatgac gtcgccggcc ttgaggtccg cctttgccgc
    16381 gggtccatcc tccgtcacct tggtgaccat ggcgcctttc gcctcggcca tgccgatcga
    16441 gtcggcgatg tcgtcggtga cgttctggat gacgacgccg agccagccgc gatcgacagt
    16501 gccatggctc tgaagctcgc tgacgacctc cttgacgagg gcggcgggga ccgcgaaggc
    16561 gataccgaca ttgccgccgc ttgggctgaa gatggcagtg ttcatgccga ccactttgcc
    16621 gtcgagatcg aagctcggac cgcccgagtt gccgcggttc acggccgcgt cgatctggag
    16681 atagtcataa ggtcccgagc cgatgtcgcg gttatgggcc gagatgatgc cggcggtcac
    16741 cgtgccgccg aggccgaacg gattgccgac ggcgagtacc cagtcgccga cccgcggcgc
    16801 cttcgacgag aactcgacgt agggaaagac cttggtccca gcatcgacct tgacgagggc
    16861 gacgtcggtg cgcggatcgc taccaatcag ctttgcggtg tattttgcgc cgccttcgag
    16921 cgtcaccgtg atgtcttcgg cgtcctcgac gacatgattg ttggtgacga tgaagccgtc
    16981 gggcgtaatg aagaagccag aaccttgggc caggctcggc gagggtttgg acggcgcctg
    17041 cggcattcct ttgcggaact gcttgaagaa gtcgtagagc gggttgtcct cgggaagatc
    17101 gggcagaccc ggaatctcca tctcgttgtc ggcgaccttg acgtcgccct tgacattgat
    17161 gctgaccacg gcggggctga ccttggccac aaggtcggcg aaactcagcg gcgcgcggcc
    17221 gtagggcgtc tcgatggtct gctgtgcacc ggtctcggcg acggccagcg gcacgctgtt
    17281 gccaagcatc agtcccaacg cgcccgtcgc gatcagggcg ccggcagcaa gcagcgtaag
    17341 tctgcgggtc ggcttccgcg cgcctgggct ctcagtcccg gagctctggt caaatctcat
    17401 aggtcacctc tcttgagaca agcaattccc gatagatagc ccggcatcag cggacgcagc
    17461 gcggcttctg gcgcgtagat ataagcgttt gccgattacg ccgccgtttc ggtggcatta
    17521 aatttcagta aggttccggc gcaatcggcc ctcggcaagg cgtccgcgcc tctcctttag
    17581 ctaatggttc aaggctcgtc gagcaagcgg tccagacggc gcttttcgcg ggcgttgaga
    17641 ggcgaagggg cttccgatgc cgccctcctc cgccgatagg cgagaaagat caggaggagc
    17701 gccgcggcca acagggcggg cgttgcgaac cacaggagca gggtttcccg cgagagccgc
    17761 ggcttgagca ggacgaactc gccgtagcgc gccactatga agtccacgac ctcgccgtcg
    17821 ctgtcgccgg ctttcagcct ctcgcgcacg agcaatctga gatcctttgc gagcggcgca
    17881 tccgaatcgt cgatggattg gttctggcag acaaggcagc gcaaacctgt cgaaatgtcc
    17941 cgcgcacggg cttcgagcgc agggtctgtc agcatctcgt cagcctggac tgctcgtgcc
    18001 ggccccgcgg caaggcaagc gatcccaagc acgagggaga cggtcgcgaa caggctgcgc
    18061 cacgacattt ccgctcactc gccggctgtg accggcgcca cctttgccgg gccgcgcttc
    18121 ggcgccccga cccgcaagcg ccggtcggat agcgatatgg cgccgccgaa gaacatgacc
    18181 agcgcgccga accagatgag gcgcacgagc ggattgaaat agagacggat gctgtaggcg
    18241 ccatctttca gcggatcgcc gagcacggca tagagatcgc cgccccagct cgcatggatg
    18301 ccggcctcgg tcgtgtcggt tcgctcaacg gtgaattggc gcttggacgg aagaagctcg
    18361 gtgacggccc tgccattgcg cgtgacggca agcacgccca cacgttcgcg gtagttcggg
    18421 ccgtcgcggg cagctacacc tttgaaggtg agcgtgtagc cggcgatgct ggcgctgtcg
    18481 ccgggcttca gcgcgaggat ctcctccgac cgccaagccg tggttgcgac gatgccgatc
    18541 accgcaagcc cgagacccgc atgagcaagc gccgagccgt aggccgcgcg cggcagattg
    18601 gcgaagcggc gccaaatctc ggcggaatcc gcctctgtga gcttcacccg gtaagcgatc
    18661 tccgtgagcg cgcccaggac gagccaaagg ccgagaccga agccgaaagg cgcaagccac
    18721 ggacctcggc cccaccatat cgcgagcagc agcagggagg ccgcaagtgc gacaaccgcc
    18781 gcggccatga gacgttgcgc cgccgcaacc atgtcccctc gcttccacgc gagcagcggc
    18841 ccgaacggca tggcgacgag gagcggaacc atgaggggca caaaggtcag attgaagaag
    18901 ggcgcgccga cggagatttt ttcaccggtg atcgcctcga gcgcgagcgg atagagcgtg
    18961 ccgacgaaga ccgcggcaca ggccgtcgtg agcaggagat tattgaacac gagcgcgcct
    19021 tcgcggctga tcggggcaaa caatccgcct tgggacagga gcggcgcgcg ccaggcgaag
    19081 agggcgaacg ctcccccgat gaagacgccc atgatggcga gaatgaagac gccgcgggcg
    19141 ggatcgacgg cgaaggtatg cacggaggtg agcacgccag agcggacgag gaaggttccc
    19201 atcaggctga gcgagaaggt gatgatggca agcaggatcg tccacacctt cagcgcgtcg
    19261 cgcttttcca tcaccagcgc cgaatggagc agcgcggtgc cagcgagcca cggcatgaag
    19321 gaagcgttct cgaccgggtc ccagaaccag aagccgcccc agccgagctc gtaataggcc
    19381 caccacgagc ccatggcgat gccgagcgtg aggcacatcc aggcggccag cgtccacggc
    19441 ctgacccaac gcgcccaggc ggcatcgatg cggccatcga tcagcgcggc gacggcgaag
    19501 gaaaaggcca tggagaagcc gacatagccc gcatagagga agggcgggtg gaaggagagc
    19561 gcggggtctt gcaggatggg gttcagcccc tccccgtcga agggggccgg gtcgaggcgc
    19621 gtgaacggat tcgatgtgac gatgatgaag aggagaaagg cggccgcgat cgagccctga
    19681 accgcgagca cgttggcttt cagctcgcgc ggcagattgg cgccataggt cgcgacgctc
    19741 gcgccgaaaa gcgcgaggat cagcacccac agaaccatcg agccttcgtg gttcccccac
    19801 acgccggaga tcttgtagag cagcggcttg gccgaatggg aattctgcca gacattcttg
    19861 accgagaaat cggaggtgac gtaagcggcc gtgagcgcgg cgaaggcgat cgcgacgagg
    19921 acgaattgca tccgcgccgc gggcacggcg atggcggcta gttgagggtc gcgcatccgc
    19981 gcgccgatca gcggcagcac ggtctgaacg atcgcgacgg cgagcgcgag gatcagagcg
    20041 tagtggccgg tctccgctat catctcctac tccgatcccc cgccctaatt caccttggct
    20101 ttcacatcgg cctcggcggg cttggcggaa gcgccttcgc gccacacgcc ttgcgccttg
    20161 agcttcttag caacttccgg cggcatgtag ttctcgtcgt gcttggcaag cacatggtcg
    20221 gcgcgaaaga cgccgtccgg ttcgagggtt ccctcggcga cgaccccttg cccctcgcgg
    20281 aacagatccg gcaggacgcc ggtataggcg acggggaggg tcttggcccg atcggtcacg
    20341 gtaaagcgga tcgtcgtccc ttcaccccgc accacgctcc cagtctccac caggccgccg
    20401 agccggaagc gggtgcccgg cgcgatcccc atcgccgcca cctcgctcgg gctatagaag
    20461 aacacaatcg agtcgcgcat ggcgaacagg acgaggccga cggcgacggc gagcacgacg
    20521 aggcacgtgc cgatcagaac gcctctacgc tgctttcgcg tcatgacttc aatccgagct
    20581 cggccgccag ccggtcaagt tcctcgagag cttggctgtt gccgctgaac gcgctcttgg
    20641 cccggccgag cgccttcacg gcgtcgtccc gacgctcgag cacagtatag gcgcgcacga
    20701 gcctgagcca gtccggaagg gacccgccct cttggtcgag ccgcgttgcg agcccttgca
    20761 ccatgcgctc gatcatcgcc tggcgctggg cgggatccat ggcttgtgcc gcggcgatgt
    20821 cggcggatgt cgggcctttt tcaccctcct ctccggccgg tgtttgcgcc gcttgttcgg
    20881 tcgcgggcgg cgtgccggca agatgcgcct ccgccgcttg catccgcttc tccaccatcg
    20941 cgcgccaggg ctcgtcgccc ttggcggtgg cgagaagccc gcgccaatct tcggcggccg
    21001 ccttgaactg cccgtcttgc tccttggcga tggcgagtag aatgcgcggc tcgatcagcg
    21061 ttgcgtcgtg tttcagcgcc tcctcgagcg acttgcgcgc ttccggcgtc accaagccgt
    21121 cattcgcgag cacgagcgct tggccctcgc cggaaagccg tttggccgag ggaccgagca
    21181 gcctgatcgc ccgcgcgtac gcttcggccg cttcgatgta gcggccccat ctgagataga
    21241 cgggcgcgat cgcctcccac ccctcgccct cctcgggatt ttgccggagg cgcgcttcga
    21301 cctttgccac gagcgcttcg acgttctggt cgctcgccgg atcctcgagc cgcgcggcga
    21361 gcggctggtc gggaaggcgc ggcgagccgt aggtgaggta gaggccgagc gacaacaggg
    21421 gaagcgccac gccgacgatc aggatccccg ctttagcggt gccgccgctg gtcttggtgc
    21481 gatcggcacc acgcgctt
//
LOCUS       DASUQP010000184        24462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_501362,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000184 DASUQP010000000
VERSION     DASUQP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 24462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24462
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501362"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFY74_08190"
     CDS             <1..445
                     /locus_tag="VFY74_08190"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493644.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HEX5931709.1"
                     /translation="DEDGFYWITGRVDDVINVAGHRMGTAEVESALVAHPKVSEAAVV
                     GYPHDVKGQGIYCYVTLMAGQDSSDQLRTELRDWVRKEIGPIASPDLIQFAPGLPKTR
                     SGKIMRRVLRKIAEDDFGNLGDTSTLADPSVVTDLIDNRQNRKSA"
     gene            complement(442..933)
                     /locus_tag="VFY74_08195"
     CDS             complement(442..933)
                     /locus_tag="VFY74_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HEX5931710.1"
                     /translation="MSVAPHASLVRFPYRPAPASVALTAKEKRLVRESFLALEPAYDL
                     VGQLFFRKLYRLDPFFRDRFAGPVEVQGRKFMAGLKLGIIAINYDDGLAPVLRLLGVR
                     HRRCGIKVRHYRIMAKALIWTLEQSLEKRFTRETKDAWSTLLTQITRIMSGSDAALLR
                     SVG"
     gene            complement(1073..1429)
                     /locus_tag="VFY74_08200"
     CDS             complement(1073..1429)
                     /locus_tag="VFY74_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931711.1"
                     /translation="MTRNLAEVVVGGKHRQAVSETKLREERIDRPGLDARAPAVIAQS
                     GGFDVIRSIRNDERNRGEAIQDLRPGIRAQEALQKLLEDEAVGEDQLTGHKRRDEANL
                     LRTHSWRGHRRGADAL"
     gene            1559..2494
                     /gene="htpX"
                     /locus_tag="VFY74_08205"
     CDS             1559..2494
                     /gene="htpX"
                     /locus_tag="VFY74_08205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5931712.1"
                     /translation="MNYFRTAILLAALTALFMGIGYLIGGQGGMLLALAVAGAMNLFA
                     YWNSDKMVLRMYGAREVDARSAPELYAMVSGLAANAGLPVPRVYIMENPQPNAFATGR
                     NPENAAVAVTTGLLRTVDRDELAGVIAHELGHIKNRDTLLMTMTATIAGAISMLANFG
                     FFFGGRSNDNGNIGFIGTLLLVILGPLAAGLVQMAISRTREYEADRAGAEISREPLSL
                     ASALQKIAAAAHRVPNIEAERNPASAHLFIVNPLSGARIDNLFSTHPNVENRVARLEE
                     MAHEMGQSGGFSRTASDVAGPWSSERRVAGRGPWV"
     gene            2523..3911
                     /locus_tag="VFY74_08210"
     CDS             2523..3911
                     /locus_tag="VFY74_08210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX5931713.1"
                     /translation="MTPSGSEARADARARDAQVGLPARRAAVELLAAVLQKKHPLDDI
                     LGRSLARGTMFDLPARDRALARAIVAASLRRKGQLDQVLGTFLARGLPEKSGTLYPIL
                     LSAAAQLIFLETPPHAAIDLAVTLAQYDPRATRYAKLANAVLRRVAGEGKAIAAGLDA
                     ARVNTPDWLWSRWTSYWGEERARKIAEAHLVEPALDLTVKCEPALWAERLSGRVLPTG
                     SVRLLPKGRIDALPGFAEGAWWVQDVAASLPARLLGEVAGKRVADLCAAPGGKTAQLA
                     LAGASVVAVDSSKTRLSLLRDNLERLGLAAETVLADAAAWRPDEAFDAVLLDAPCAST
                     GTIRRHPDIPYVKSAGDIATLTGLQARLLDNAARIVKPGGRIVYSTCSLEPEEGEAQI
                     AALLVRNATLGLEAIEEDELFGQVAWIEPSGCLRTFPYDLKLDSPEWSGMDGFFAARL
                     VRAGEPRPSHRA"
     gene            4009..5793
                     /locus_tag="VFY74_08215"
     CDS             4009..5793
                     /locus_tag="VFY74_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX5931714.1"
                     /translation="MAADSQPNERGGRGPAPRLGGPGAFRRFMLASMASVTYPGSKAE
                     QLLLAPQELRTADPSFATELYNGHLGLAGRLADVEAASPFEIAPPSEAWARELYGFGW
                     LRHLRAANSELSREQAKVLVQDFIALKSAHHALAWKPEIVGRRVISWLSNSVLVLDSG
                     EPEGYEAFLRALTSQLRYLSASYRDAPDGVPRLVALMALIYAGLCIAEQQAVVDRYSR
                     PFSRELNRQILADGGHISRNPDALVELLLDLLPLRQCFVARDRPPPKELTDAIDRIMP
                     MLRFFRTADGTMARFNGAGPTATDALATVLAYDDVEGTPVRAAANSGYVRLTCGSTML
                     ICDIGPAPAPSLSSSAHAGFLSFEMSSANCPMIVNCGSPSLDHEEWRPFSRSTAAHST
                     LTFEDLSSAAFAPPDANGSEVAGDAPLSGPVNAQATFADQGDNYRIKGSHDGYLAEFG
                     VSHARQLLIAPTGLLLSSEDKLSAPIGLKSPEGDLIAGSYAIRFHLHPSVRAAKAADG
                     QSVTLTLKNGETWKISSNAPETKIEESFFLADARGPQATSQVVLSGLMGEASEVRVVW
                     NIERMTSGDGGGNHLVDPNDLAPAPAAA"
     gene            5799..7385
                     /gene="purH"
                     /locus_tag="VFY74_08220"
     CDS             5799..7385
                     /gene="purH"
                     /locus_tag="VFY74_08220"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX5931715.1"
                     /translation="MERRKITRALISASDKTGLADFARALRAHGVEIVSTGGTAKALR
                     EAGIAVTDVAEITRMPEMMDGRVKTLHPRIHGGLLAVRGNEAHERALAEHAIPAIDLL
                     AVNLYPFEATVEGGANFETCIENIDIGGPALIRAAAKNHDSVTVVVEPQDYARVLAEI
                     AANDGATTLPLRQALAAKAYARTASYDAAIAAWLAEKLGDRTPVTRAISGTGGEALRY
                     GENPHQWAAFYRGGERRFGVTTATQAQGKELSYNNLNDTDAAYELVAEFDPAASAAVA
                     IIKHANPCGVATAKTLREAYEKALRCDPVSAFGGIIALNRALDAGAAREIVKIFTEVI
                     IAPDASDEARAIVAEKKNLRLLLAGGLPDPTSDGLSFRSLSGGFLVQSRDSGVVANGE
                     MKVVTKRKPSKEELADLFFAFTVAKHVKSNAIVYARDGATVGIGAGQMSRVDAARMAA
                     WKADEAAAAQGLGQSLTIGSVAASDAFFPFADGIKLIAEAGATAIIQPGGSMRDDEVI
                     AAADAAGLAMVFTGMRHFRH"
     gene            7548..7748
                     /locus_tag="VFY74_08225"
     CDS             7548..7748
                     /locus_tag="VFY74_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931716.1"
                     /translation="MTTVEDIEEAVSKLTPEQLAKFRAWFEAFDAQIFDEKIERDATS
                     GKLDRLADDAVRAHRKGFAREL"
     gene            7745..7999
                     /locus_tag="VFY74_08230"
     CDS             7745..7999
                     /locus_tag="VFY74_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931717.1"
                     /translation="MRHLASPRFWEAYKGLPENVRELADKSFHLLKQNPRHPSLHFKR
                     AGRFWSVRVGLHYRALAVPVDSDLVWFWIGTHADYDAMME"
     gene            complement(8028..9428)
                     /locus_tag="VFY74_08235"
     CDS             complement(8028..9428)
                     /locus_tag="VFY74_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5931718.1"
                     /translation="MPTGGLERRRGVPRDAAEHAGKRGLFGWVLFDWATQPFYTLVVT
                     FLFAPYFVNGFMADPARGAALWAYATGIAELIAAALAPVLGAIADAGQPRKPWIAAFS
                     AMLVVGLFGLWLAAPGRTDLVPVVLIAFGVAIIGAELATVFTNAMMTSLVSDKRLGTL
                     SGAGWAAGYIGGLVSLALVAGLLVADPATGKTLLGLPPLVPLDPGTREGDRLVGPFSA
                     IWYLVFVLPLFLFTPDRRAAPLQPRAVTAGLTQLVRSVKDLVRDDAQVALFLLARMLY
                     ADGLGAVFAFGGIYAATVFGWGATELGLFGIVLTLAGTIGAALGGPIDDARGSKRVIV
                     FTLGLFVLAAIGVLSVDRESVLFVLPVEAKVPGNAPFASTGEQVYLAFAILIGLASGP
                     IQSSSRALLARMSPPGKITEFFGFFSFSGKITAFAAPLLIGAVTQATGSQRLGIASSL
                     AFLLAGLLLLLRVNAK"
     gene            complement(9409..10059)
                     /locus_tag="VFY74_08240"
     CDS             complement(9409..10059)
                     /locus_tag="VFY74_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931719.1"
                     /translation="MVAPSSMQPFTPEDRAAISAAITRAEQKTSGEIVVVAAIASDGY
                     RSFGLLWAALLSLAVPIPLVFATKWPVQYIYLLQLAAFLTFLVMFQLEGIRHRLVPPA
                     IKRARAHQRAVEQFLVQNMHTTKGRTGVLIYVSFVERFAEVIADEGIYRKVAPSAWED
                     TVGELTNHLARGTTTEGFIRAVERCGKILAKHFPPGRVADNELPNHLIVLDAYGRP"
     gene            complement(10053..10841)
                     /locus_tag="VFY74_08245"
     CDS             complement(10053..10841)
                     /locus_tag="VFY74_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX5931720.1"
                     /translation="MAIVRRFGGMLIATALALLMSPAAAEPVFPELTGRVVDDANLLT
                     YPEKVAVTADLKALEDKSSDQLVVVTLPSLQGYAIEDFGYRLGRRWGIGTEKLDNGVL
                     LIVAPNERKVRIEVGTGLEGTLTDALSRIIIENSILPRFREGDFGGGIKNGVRDIVLV
                     LTGDAAEVEARARARIDADDPMIGWLFIILWVAIFFWIAYSMWRSARYAAPAGRSGTA
                     GPILLPGPTWDGGGGGGWSGGGGGGGGFSGGGGSFGGGGASGGW"
     gene            complement(10865..11443)
                     /locus_tag="VFY74_08250"
     CDS             complement(10865..11443)
                     /locus_tag="VFY74_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5931721.1"
                     /translation="MLRLACLLLAGVVLAGCGINTIPTLQEQAKAAWSQVLNQYQRRA
                     DLIPNLVETVKGYAAQESKVLIEVVEARAKATQVQIPPDILTNPDAFQKFQDAQNNLT
                     GALGRLLLIVENYPDLKSNQNFLALQSQLEGTENRISVARQDYITAVRDYNTEIRTIP
                     GRWWRALLYPDAEPMQNFTAAEGTEKPPEVKF"
     gene            11942..14092
                     /locus_tag="VFY74_08255"
     CDS             11942..14092
                     /locus_tag="VFY74_08255"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5931722.1"
                     /translation="MRIAFLRERAAKAAKRTSSTLFAFLGVAVVAAAVGASMPAEAKK
                     QKNGNKQVEAAIPDPADGEPMTLVVSLSQQKVDVYRGVTLITSSKVSTGMRGYATKAG
                     VFSILEKRRHHHSNLYSGAPMPWMNRITWSGTALHAGVVPGYPASHGCIRLPFSFAPK
                     LFKITTVGDNVIVARDRPVPQLIAHANLFQPLRTPAAPAVVKQEEPPKRQSSNEAVPM
                     APVNTYPGKLARADVPGTTTDVPSSLEAEAIATKTASPNNADPAAIQDTHTHAFDPFA
                     GAEPKASARDGHATDRSAKASGHALDEDAGSGRSLAKVKAVPVKPLPAIEIAAAQAAP
                     AAAAPAEALKPVEPVVSTLAPAAQPPKPAESGTASAVPAEAVSPAEPTPADAASAANK
                     VEAAGMTAGPAAVEQARVTAEPAPASTPTAPAAGQALALATPPQASPAEASALPQLDA
                     PPSAVAARLMAGAKEAALLAAEPRSTAPLRILVTRRTQRDRIIGVQRIFADMGYLEQQ
                     EFDGTLGKATVGAIKAFQKANGMPETGALNEELAKKVYEVSGKGEPPAGHLYVRQEFG
                     RVFDAPVTFKDPETPLGTHIYTALKFGPGDSSVRWTSMSLQGNDSAAALDRLDIPSDV
                     RQKISERLTPGSSLIIGDTAINTAGLTKGNDFVVWAKETPAKITAASLDGDFTPAKPR
                     KKKRVRAVRRPYFDYGYDRYQRPPGFSRGPAWPW"
     gene            complement(14202..14276)
                     /locus_tag="VFY74_08260"
     tRNA            complement(14202..14276)
                     /locus_tag="VFY74_08260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14242..14244),aa:Gly,seq:gcc)
     gene            14434..14907
                     /locus_tag="VFY74_08265"
     CDS             14434..14907
                     /locus_tag="VFY74_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEX5931723.1"
                     /translation="MPADRKTLDARLAALGIATTTVEHEPVFTVEESAALRMSIPGAH
                     TKNLFLTDPDGRMVLVTAKDDTRVDLKALAKRLGAERFSFGKAPRLEAVLGVSAGSVT
                     PLALINASAAGVTVVVDQALLDYEEVNCHPLENTATMRLATADLIRFIKECGQSR"
     gene            15017..15934
                     /gene="trxA"
                     /locus_tag="VFY74_08270"
     CDS             15017..15934
                     /gene="trxA"
                     /locus_tag="VFY74_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5931724.1"
                     /translation="MSVETPILKQGGAGTPDAELIKNTTTKDFMRDVVEASREVPVLV
                     DFWAPWCGPCRQLTPILEKAVRAAKGAIKLVKLNIDEHPQIPGQMGVQSIPAVFAFQD
                     GKPVDGFMGALPESRVNAFISRLVGDSVADAAADLEVAEAALAAGDLNTAAQTFGEIL
                     QADRENAQALAGLAKCYIRTGDLARAEQTLALVPPAKAESAPVASARAALELQRKSAS
                     VDDIDKLRAAVAADPNDAQSRFDLALALNAKGERAAALEELLAIIAKNRAWNDDAARK
                     QLLQLFDAWGAADPATVAGRQRLSSLLFA"
     gene            15953..16636
                     /locus_tag="VFY74_08275"
     CDS             15953..16636
                     /locus_tag="VFY74_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5931725.1"
                     /translation="MGITDRYLTVADMPQMLAMFPLRGAILLPRASLTLNVFEPRYLA
                     LVDHALAADRLIGVVQPAPEAGDAESPKGRSFPLRSVGCAGRLFSFTESDDGRVVIAL
                     AGIARFTITGDVAGDTPFRICQVDFSPFAKDFVTGHGEDDVDRPRLLATLKNYLTASN
                     LNADWDRIDGASSERLVNTLSILSPYGPEEKQALLEAPDLRARAEALVALAEMELAAR
                     DDGSGTSIQ"
     gene            16650..16850
                     /locus_tag="VFY74_08280"
     CDS             16650..16850
                     /locus_tag="VFY74_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX5931726.1"
                     /translation="MTNERGGERRLDPKLLEILVCPLTKTSLDYDPERQELISRAAGL
                     AYPIRDGIPIMLPEEARKLDQS"
     gene            complement(16847..18103)
                     /locus_tag="VFY74_08285"
     CDS             complement(16847..18103)
                     /locus_tag="VFY74_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5931727.1"
                     /translation="MPDGRTRGLERFDVAVAGGGLLGRTLALALGRLAPQGFRIALID
                     AELAETGRSGMEDARSLALSAATKNLLSALDLWAELAPSVQAIEAIEITDSALDAALR
                     PHLLGFDEELGESGTSAFMAEHGDLDRVLAEAVAVEPAVAVYPSDRVTDFNAGPFAAE
                     VSLASGMTIAASLLVAADGKRSHLRERAGIKCVGWSYPQVGIVTTVAHERPHHGKAVQ
                     HFLPAGPFAILPLTGNRSSIVWTEDAAEGAAIMAAGEARFLEELTKRFGHRLGAISLA
                     GLRQSFSLDMQIARSFVADRFALIGDAAHVVHPLAGQGLNIGMRDVAALTETVVETAR
                     LGLDIGSAVPLERYVRWRRFDSAFSAAVMDGLNRLFSNDSAPLRTLRDLGLGLVDRAP
                     ALKRFLVSEAAGRSGTVPSLLTGQRF"
     gene            18182..19048
                     /gene="tesB"
                     /locus_tag="VFY74_08290"
     CDS             18182..19048
                     /gene="tesB"
                     /locus_tag="VFY74_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX5931728.1"
                     /translation="MSRALEQLLSILELEQLEVNLFRGLSPQVGWQRVFGGQVIGQAL
                     VAAQRTVVDRLAHSLHAYFLRPGDPAVPIIYNVDRIRDGGSFTTRRVVAIQHGHAIFS
                     MAASFHKEEPGLQHQTSMPDVPPPEALPSEAELKAKLIDKLPPQVKIYWERERPIEIR
                     PVDLSRYLSPQNRAASQQVWIRATGKLGDDLALHQCVLAYASDFTLLDTALIAHGRFI
                     FDPRLMLASLDHALWFHRRFRSDDWLLYAQDSPSSGAGRAFCRGTLFTRDGVLVASTA
                     QEGLLRERQPGS"
     gene            19268..19606
                     /locus_tag="VFY74_08295"
     CDS             19268..19606
                     /locus_tag="VFY74_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5931729.1"
                     /translation="MKLVMAIIKPFKLDEVRQALTELGLQGMTVTEVKGYGRQKGHTE
                     IYRGAEYAVNFLPKIKIEVVIPTKMVDKAVEGIVAAAKTGQIGDGKIFVVPVEQTVRI
                     RTGETNESAL"
     gene            19615..20961
                     /locus_tag="VFY74_08300"
     CDS             19615..20961
                     /locus_tag="VFY74_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5931730.1"
                     /translation="MKMTLAKYLSSAVLALAALLIAKLDPALAQEAAEAAAEAPKPAI
                     DSGDTAWMLTSTALVLMMTVPGLALFYGGMVRKKNVLSIIMQCFATTCIVTVAWMVLG
                     YSWAFTDGGGMQSYLGGMSQFMLKSMTIDSTNSLAATIPESVFMTFQMTFAIITPALI
                     VGAFADRMKFSALVLFMTLWVLFVYAPIAHWVWGGGFLGGAGVLDFAGGTVVHINAGV
                     AGLVTALYLGKRSGYGSENMAPHNLVYSLTGAALLWVGWFGFNAGSAVAANGRAGMAM
                     AVTQIATATAALGWMFAEWLVHRKPSVLGIISGAVAGLVAITPASGFVGPVGALWIGA
                     AAGVGCFFAATTVKRAIGYDDSLDVFGVHGIGGIIGAILTGVFAVEAIGGAKGALEGN
                     VGQIWTQIEGIAATIAWSGLATLVILVVVNILTGVRVSQAVEVEGLDINLHGEVVP"
     gene            21099..22307
                     /locus_tag="VFY74_08305"
     CDS             21099..22307
                     /locus_tag="VFY74_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5931731.1"
                     /translation="MSPRDMPTYLDDVVRSPFVRLAALLEGISPGAPPIDLSLGEPKA
                     LIPPFLGPTLQNHLGEFGRYPPIKGIPSLRNAIADWMGRRYPILKGAIDPEQHILPLN
                     GSREGLFSAIFPAKARKPQLDEPAVLIPNPFYQAYAAAAAASGAPPVFLTARAEDGFL
                     PRLDDIDAALLQRTAAFYLNTPSNPQGAVADCSYLAKATALARRFDFLLFADECYSEI
                     YGETPPPGALETAWAETRSLSNVVSFQSLSKRSGLPGLRSGFVAGDPVFSAAFARFRN
                     VACPQVPLPVQHVSAEAWADERHVEEGRALYRRSFAIADELLDGRYGYRRPQGSFFLW
                     LKMAVFGGGEAAAKTLWKGCGVRVLPGTYLAQAGPDGVNPGIDHVRIALVHDAETTRE
                     ALTRVVATLG"
     gene            22312..>24462
                     /locus_tag="VFY74_08310"
     CDS             22312..>24462
                     /locus_tag="VFY74_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX5931732.1"
                     /translation="MASRGRSKARRRLLPGAVETGLRRIMFGGYGLLFVTIACAAWAS
                     LATWSVHDPSLNNATRAVPRNFLGSWGAVLADLTIQSLGLAAIILFLPLAAWGWHLVF
                     STTPDRRRRRFLAWPASVILLAAAFAAFPQPKSWPLPNGLGGILGDFFMAGAHVIGPF
                     LPEAAVSFVAGLAFFVIGTMLLLFACGTSTSRLIALWAPQSGVAGEWANASLGAAMHV
                     AMHAGARLRHTFRGRDAFNEEIEDRLDEGADDEDVEPARRLDADGRIEPSFGPAPIGE
                     AFDEDEGDDDITSPNYRITRLNPQKKRKHTGLARQTHSDEPYEPPSIKLLQPPKGSKG
                     RGVSDEVLQENARELEGVLQDFGVKGEITNVRPGPVVTLYELEPAPGTKSSRVIGLAD
                     DIARSMSAVAARVAIVSGRNAIGIELPNDRREIVVLREMIESPDFEETDARLALALGK
                     NIGGEAIIVDLARMPHLLIAGTTGSGKSVGINTMILSLLYRLSPDQCKFIMIDPKMLE
                     LSVYDGIPHLLAPVVTDPKKAVVALKWTVREMEERYKRMSKLGVRDIKGYNSRILQAE
                     AKGEVLTRTVQTGFDRATGRAIYEQEEMDYDAMPFIVVIVDEMADLMMVAGKDIEAAV
                     QRLAQMARAAGIHLITATQRPSVDVITGTIKANFPTRVSFQVTSKIDSRTILGEQGAE
                     QLLGQGDMLFMAGGGRIQRVHGPYVADTEVEEIVA"
BASE COUNT         4270 a         8168 c         7982 g         4042 t
ORIGIN      
        1 cgacgaggac ggcttttact ggatcacggg gcgcgtcgac gacgtgatca acgtcgccgg
       61 ccatcgcatg ggcacggcgg aagtcgagtc ggcgctcgtc gcccacccca aagtgtcgga
      121 agccgcggtc gtcggctacc cccacgacgt caagggccag ggcatctact gttatgtcac
      181 gctgatggcg gggcaggatt cttccgatca gctcagaacc gagctgcgcg actgggtgcg
      241 caaggagatc gggccgatcg cctcgccgga cctcattcag ttcgcgccgg gcctgccaaa
      301 gacgcgttcg ggaaagatca tgcgccgcgt cctgcgcaag atcgcggagg acgacttcgg
      361 caatctcggc gacacctcga cgctcgccga cccgtcggtg gtcaccgacc tgatcgataa
      421 ccggcagaat cggaaatcgg cttagcccac gctccgcagg agagccgcgt cgctcccgct
      481 catgatgcgg gtgatctggg tgaggagagt cgaccaggcg tccttcgtct cgcgcgtgaa
      541 gcgcttctcg agcgactgct cgagcgtcca gatcaacgcc tttgccatga tgcggtaatg
      601 gcgaaccttg atgccgcagc gccgatgccg gacgccgagc agcctgagca cgggtgcaag
      661 accgtcgtcg tagttgatcg cgatgatgcc gagcttgagc cccgccatga atttgcgccc
      721 ttggacctcg accgggccgg caaagcggtc gcgaaaaaac ggatcgaggc ggtagagctt
      781 gcgaaagaag agctggccga cgaggtcgta ggcgggctcg agcgcaagga agctctcgcg
      841 aacgaggcgc ttctcctttg ctgtcagcgc gacgctcgcc ggcgcggggc ggtacgggaa
      901 cctgacaagg ctggcgtgag gggcgactga catggcgcga cgacctccag ggctgcttcg
      961 tcagagcatg cccggggagc cgtttaggaa gcgttaaggt aaccgcggcg gaaaccaact
     1021 gcctgacgcg gggccggaaa cggccccttg ccctgacgcc gcccgatagg cttcagagcg
     1081 cgtcggctcc cctgcgatga ccccgccagg agtgtgtcct caagagattt gcctcgtcgc
     1141 gccgtttgtg gccggtgagc tgatcctcac caacggcctc gtcctccagg agcttctgca
     1201 aggcttcttg ggcgcgaatg cccgggcgca gatcttggat cgcttctccg cgattcctct
     1261 catcgttccg gatcgaacgg atcacatcga agccgccgct ttgcgcaatc actgccggcg
     1321 cgcgggcgtc caggccggga cgatcgatgc gctcctcgcg cagctttgtc tccgacacgg
     1381 cctgacgatg ctttccgccg acgacgactt ccgccagatt gcgggtcaat gcgccttgaa
     1441 gctctggggt gcaccgacct gaatcctcac gacctccttg ccgcgacgac tttgggtccc
     1501 aacataaaaa gggcgcgcca accggcgtcg ccgtaatgcg ttggaggccc tgaacgctat
     1561 gaactatttc cgcaccgcca ttcttcttgc ggcgctgacg gcgctgttca tggggatcgg
     1621 ctatctgatc ggtggccaag gcggcatgct gcttgcgctt gccgtggcgg gcgcgatgaa
     1681 cctgttcgcc tattggaact ccgacaagat ggtgctccgc atgtatgggg cgcgcgaagt
     1741 ggacgcgcgt tcggcgcccg aactctacgc gatggtgagc gggcttgccg ccaatgccgg
     1801 cctgcccgtg ccgcgcgtct acatcatgga gaatccgcag ccgaacgcgt ttgccaccgg
     1861 gcgcaatccg gagaacgcgg ccgtcgcggt cacgaccggc ctgctcagga cggtcgaccg
     1921 agacgagctt gccggcgtca tcgcgcatga gcttggccac atcaagaacc gcgacacgct
     1981 cttgatgacg atgacggcca ccatcgcggg cgcgatctcg atgctcgcca atttcggctt
     2041 ctttttcggc ggccgaagca acgacaacgg caatatcgga tttatcggca cgctcctcct
     2101 cgtgattctc gggccgcttg ccgcgggctt ggtgcagatg gcgatcagcc gcacccgcga
     2161 atacgaggcc gaccgcgccg gggcagagat cagccgggag cccctgtcgc ttgcgtccgc
     2221 cctgcaaaag attgcggccg ccgcgcaccg cgtccccaat attgaggccg agcgcaatcc
     2281 cgcgtccgcc cacctcttca tcgtcaaccc gctctcgggc gcgcgcatcg acaatctgtt
     2341 ttcgacccac ccgaacgtgg aaaaccgcgt ggcgcgtctc gaggagatgg cgcatgagat
     2401 gggccaaagc ggtggctttt cgcgcaccgc gtccgacgtg gcggggccat ggtcctcgga
     2461 gcgccgggtt gccggacgcg ggccctgggt ctagaccctc aagctcaggc agttttggtc
     2521 acatgacacc ttccggcagc gaagcgaggg cggacgcacg cgcgcgcgac gctcaagtcg
     2581 ggctccccgc acggcgcgcc gcggtcgagc tgcttgccgc tgttctccag aagaagcatc
     2641 cgctcgacga catccttggc cgctcgcttg cgaggggcac gatgttcgat ctgccggcgc
     2701 gagaccgcgc gcttgcccgc gccatcgtgg cggcaagcct gaggcgcaag ggccagctcg
     2761 accaggtgct cggcacgttc ctcgcgcgcg ggcttccgga gaagtccggc acgctctatc
     2821 cgatcctgct gtcggccgcg gcacagctca ttttcctgga gacgccgccg catgcggcga
     2881 tcgatctcgc cgtgacgctc gcccaatacg atccgcgagc gacccgctat gccaagctcg
     2941 ctaacgccgt tctccgccgc gttgccggcg aaggcaaggc gatcgcagcg gggctcgacg
     3001 cggcgcgggt caatacgccc gactggctgt ggtcgcgctg gacgagctat tggggcgagg
     3061 agcgtgcacg aaaaatcgcg gaagcgcatc tcgtcgagcc cgcgctcgac ctcacggtca
     3121 agtgcgagcc tgcgctctgg gccgagagac tttccggccg tgtgttgccc acgggcagcg
     3181 tgcgtctgtt gcccaagggg cgcattgacg cgcttcccgg cttcgcggag ggcgcgtggt
     3241 gggtgcagga cgttgccgcc tctctgccgg cgcggctcct cggcgaggtg gccggcaagc
     3301 gcgtcgccga cctctgcgcg gcgcccggcg gcaagaccgc tcagcttgct ctcgccggcg
     3361 cttcggtcgt ggccgtcgat agctcgaaga cgcgcctttc cctgcttcgc gacaacctcg
     3421 agcgcctcgg gcttgcggct gagacggtgc tggcggatgc cgcggcctgg cggccggacg
     3481 aagcgttcga tgcggtgctg ctcgatgcgc cctgcgcgtc gacagggacc attcgccggc
     3541 atcctgatat cccctatgtg aaatcggcgg gcgatatcgc aacactcacg gggctccagg
     3601 cccggttgct cgacaatgcc gccaggatcg tcaagccggg gggtcggatc gtctactcga
     3661 cctgctcgct cgagcctgag gaaggcgagg cccagatcgc cgctctgctc gtacgcaacg
     3721 cgactctcgg cctcgaagcc attgaggaag atgaactttt cggccaggtg gcgtggatcg
     3781 agccctcggg ctgcctcaga acttttccct atgatctcaa gctagactcc cccgaatgga
     3841 gcggcatgga cgggtttttt gccgcgcgcc tcgtcagggc cggcgagccg agaccctcgc
     3901 atcgggcttg atcggaacgc gtccccgccc gacaatgccc tcctctggat gcacggggag
     3961 gccgcgcgcc cagtcattgc gcgtggcggc agcttggaga caaggaacat ggcggctgac
     4021 tctcagccca acgagcgcgg cggtcgcggt cccgcgccgc gtctcggcgg cccaggcgcg
     4081 ttccgccgct tcatgctcgc gagcatggcg agcgtcacct accccggcag caaggccgag
     4141 cagctcctgc tcgcccccca ggaactcagg accgccgacc cgagcttcgc caccgagctc
     4201 tacaacgggc atcttgggct tgccgggcgc ctggccgacg tcgaagccgc ctccccattc
     4261 gagatcgcgc cgccgtcgga ggcttgggcg cgcgagcttt atggctttgg ctggctcagg
     4321 catctgcgcg ccgcgaactc ggaactgtcg cgcgaacagg cgaaggtgct ggtgcaggat
     4381 ttcatcgcgc tgaagagcgc ccatcacgct ctcgcctgga agccggagat cgtcgggcgc
     4441 agggtgatct cgtggctttc caattcggtg cttgtgctcg atagcggcga gccggagggc
     4501 tatgaggcgt tcctcagggc gttgacctct cagctccgct atctctcggc gagctatcgc
     4561 gacgcgccgg acggcgtgcc gcggctcgtg gcgctgatgg cgctgatcta tgccgggctc
     4621 tgcatcgccg agcagcaggc ggtggtcgat cgctattcgc gcccgttctc acgggagctc
     4681 aaccggcaga tcctcgccga cggcgggcat atctcgcgca atcccgacgc gctggtcgag
     4741 ctcctgctcg acctcttgcc gctcaggcag tgtttcgtgg cgcgcgaccg cccgccgccg
     4801 aaagagctga ccgacgcgat cgatcgcatc atgccgatgc tccgcttctt ccgcaccgcc
     4861 gacggcacga tggcgcgctt caacggcgca ggtcccaccg ccaccgacgc gctcgcgacc
     4921 gtgctcgcct atgacgacgt ggaaggaacg ccggtccgcg cggcggcgaa ttcgggctat
     4981 gtgcggctca cctgcggatc gaccatgctg atctgcgata tcggccccgc gcccgcgccc
     5041 tcgctcagct cgagcgcgca tgccggattc ctctccttcg agatgagctc tgccaattgt
     5101 ccgatgatcg tcaattgcgg gtcgccctcg ctcgatcacg aagagtggcg ccccttttcc
     5161 cgctcgaccg cggcgcattc gaccctgacc ttcgaggatc tgtcctcggc ggcgttcgcg
     5221 ccgccggacg caaatggcag cgaggtggcg ggcgatgcgc cgctcagcgg tcccgtcaac
     5281 gcgcaagcca cattcgccga ccagggcgac aattacagga tcaaggggtc tcacgacggc
     5341 tacttggctg aattcggcgt gagtcacgcg cgccagctcc tcatcgcgcc gaccggactc
     5401 ctcctctcga gcgaggacaa gctctccgca cccatcgggc tgaaaagccc cgagggcgat
     5461 ctgatcgccg gttcgtacgc catccgcttc cacctgcatc cgagcgtgcg cgcggcgaag
     5521 gcggctgacg ggcagagcgt cacgctcacg ctcaagaacg gcgagacctg gaagatcagc
     5581 tcgaacgctc ccgaaacgaa gatcgaggaa agctttttcc ttgccgatgc gcgcgggccg
     5641 caagccacgt cgcaagtcgt gctctccggc ctcatgggcg aggcgagcga agtccgcgtc
     5701 gtgtggaata tcgagcgaat gacttccggc gacggcggcg gaaaccatct ggtcgatccc
     5761 aacgacctcg cgccggcgcc cgcggcagcc tagaccgcat ggaacggcgc aagattaccc
     5821 gtgcgctgat ttcggccagc gacaagaccg gccttgccga tttcgcgcga gctttgcgcg
     5881 cgcacggcgt cgagatcgtg tcgacgggtg gcacggcgaa ggcgttgcgc gaggccggga
     5941 tcgccgtgac cgacgtggcc gagatcacgc gcatgcccga aatgatggac gggcgcgtca
     6001 agacgctgca tccgaggatt cacggcggtt tgctcgccgt ccgcggaaac gaagcgcatg
     6061 agcgggcgct cgccgagcac gcgatccccg ccatcgacct gcttgcggtc aatctctatc
     6121 cgttcgaggc gacggtggag ggcggcgcca acttcgaaac atgcatcgag aatatcgaca
     6181 tcggcggccc ggccctgatc cgtgccgcgg cgaagaacca cgacagcgtc accgtcgtgg
     6241 tcgaacctca agactatgcg cgcgtgcttg ccgagattgc cgcgaacgac ggcgccacca
     6301 cgcttcctct gcgccaggcg cttgccgcga aggcttatgc acggaccgct tcttacgacg
     6361 ccgcgatcgc cgcgtggctt gcagaaaagc tcggcgaccg gacgcccgtg acgcgcgcga
     6421 tttccggcac aggcggcgaa gcgctccgct acggcgagaa cccgcatcaa tgggctgcct
     6481 tctatcgcgg aggtgaacgg cgcttcggtg tcaccacggc gacgcaagcg cagggcaagg
     6541 agctgtccta caacaacctc aacgacaccg acgcggccta cgagctcgtt gccgagttcg
     6601 atccggcggc aagcgcagcc gtcgccatca tcaagcacgc aaacccttgc ggcgtggcga
     6661 ccgcaaagac gcttcgcgag gcctacgaga aggcgcttcg gtgcgatccg gtcagcgcct
     6721 tcggcgggat catcgcgctc aatcgcgcgc tcgatgccgg cgccgcgcgc gagattgtca
     6781 agatcttcac cgaggtgatc atcgcgccgg acgcgagcga cgaagcccgc gcgatcgtcg
     6841 ccgagaagaa gaacttgcgg ctcctgcttg caggcgggct ccccgatccg acgtcggacg
     6901 ggctctcgtt ccgctcgctc tccggcggct ttttggtgca gtcgcgcgat agcggcgtcg
     6961 tcgcgaacgg cgaaatgaag gtcgtgacca agcgcaagcc gagcaaagag gagctggccg
     7021 accttttctt cgcgttcacc gtggcgaagc atgtcaaatc aaacgcgatc gtctatgcgc
     7081 gcgacggcgc cacggtcggc atcggcgcag ggcagatgtc acgcgtcgat gcggcgcgga
     7141 tggcggcgtg gaaggcggac gaggcggctg cggcgcaagg gcttggccag agcctgacga
     7201 tcggctccgt cgcagcgtca gacgcgttct ttcccttcgc cgatgggatc aaattgatcg
     7261 cggaagccgg cgccacggcc atcatccagc ccggcggctc gatgcgcgac gacgaggtga
     7321 tcgccgcggc cgacgcggcg gggcttgcca tggtgttcac cggcatgcgc cacttccggc
     7381 attagcaagc cctgctccct ctttcggatt cgtcatccct gaggtggccg cgaagcggcc
     7441 ctcgaaggac gcacctagaa ttcgtcatcc ttcgaggcta gcatctcagg ttgacggcac
     7501 tcgggcgcgc gaggtgtcct cgcggaaccg atgtgctatg attgcttatg actacggtcg
     7561 aagacatcga ggaagccgtg agcaagctta cgcccgagca gcttgccaag ttccgtgcct
     7621 ggttcgaagc gtttgacgcg cagatattcg acgaaaagat cgagcgcgac gccacgtccg
     7681 gcaagctcga tcggttggcc gacgatgctg ttcgggctca tcggaagggt tttgcccgcg
     7741 aactatgagg catctcgcca gccccaggtt ctgggaggct tacaagggct tgcctgagaa
     7801 tgttcgcgaa cttgccgata agagcttcca cctgctcaag cagaaccctc ggcatccgtc
     7861 gttgcatttc aaacgcgccg gtcgcttttg gtcggtccga gtcggtctgc actatcgggc
     7921 tctggccgtg ccagtcgact ccgacttggt ttggttctgg atcggcacgc atgcggacta
     7981 cgacgcgatg atggaatagc cgagccgatt gccaaagagg ggaaaagcta cttcgcgttg
     8041 acacggagca agagcaggag gccggcgagg agaaaggcga ggctcgaggc gatgccgagc
     8101 cgctggctcc ccgtcgcttg cgtgacggcg ccgatgagaa gcggcgcggc gaacgcggtg
     8161 atttttcccg agaaggaaaa gaagccgaag aactcggtga tcttgccggg cggactcatc
     8221 ctcgcgagca gcgcgcggct cgacgactgg atcggacccg aggcgagccc aatcagaatc
     8281 gcgaaggcga gatagacctg ctcgccggtg gatgcgaacg gcgcgttccc cggcaccttc
     8341 gcttccacgg ggagcacgaa cagaacgctc tcgcgatcga ccgacaggac gccgatcgcg
     8401 gccaagacga acaatccgag tgtgaagacg atgacccgct tcgaacctct tgcatcgtcg
     8461 atcggcccgc cgagcgcggc gccgatcgtg ccggcaagag tgaggacgat gccgaacagg
     8521 ccgagctcgg tcgcgcccca gccgaacacg gtggcggcat agatgccgcc gaaggcgaac
     8581 acggcgccga ggccgtcggc gtagagcatg cgcgcgagaa ggaacagcgc gacctgcgcg
     8641 tcgtcgcgca cgagatcctt caccgagcgc acaagctgcg taagacccgc cgtgaccgcg
     8701 cgcggttgaa gcggcgccgc gcgccggtcg ggcgtgaata gaaagagcgg gagcacgaac
     8761 acgaggtacc agatggccga gaatggaccg acgagacggt cgccctcgcg cgttccggga
     8821 tcgagcggca cgagcggcgg aagtccgagc agggtcttgc cggtcgccgg atcggcgacg
     8881 agaaggcccg cgacgagtgc caagctgacg agaccgccga tatagcccgc cgcccagcct
     8941 gcgcccgaca gcgttccgag cctcttatcc gagacgagcg acgtcatcat ggcgttggta
     9001 aagacggtcg caagctcggc gccgatgatg gcgacgccga aggcgatgag cacgaccggc
     9061 acgagatcgg tgcggccggg cgccgcgagc cacagcccga atagcccgac gaccagcatg
     9121 gcggaaaagg cggcgatcca gggcttgcgc ggctggcccg catcggcgat ggcgccgagc
     9181 acgggcgcga gcgcggctgc gatcagctcg gcgatgccgg tggcgtaagc ccagagcgcc
     9241 gcgccgcgcg cgggatccgc catgaagccg ttgacgaaat agggcgcgaa caggaaggtg
     9301 acgacgagcg tgtagaaggg ctgcgtcgcc caatcgaaca gcacccagcc gaacagaccg
     9361 cgctttcccg catgctcggc cgcatcgcgc ggcacgccgc gccgtcgctc aaggccgccc
     9421 gtaggcatcg agtacgatca gatgattggg aagctcgtta tcggccaccc ggcccggcgg
     9481 gaaatgcttg gcgaggatct ttccgcaacg ctcgaccgcg cggatgaagc cttcggtcgt
     9541 ggtgccgcgc gcgaggtgat tggtgagctc gcccaccgtg tcctcccagg ccgaaggcgc
     9601 caccttgcgg tagatgccct cgtccgcgat gacctcggcg aagcgctcga cgaaggagac
     9661 atagatgagc acgcccgtgc gccctttcgt ggtgtgcatg ttctgcacca ggaactgctc
     9721 gacggcgcgc tgatgggcgc gggcgcgctt gatggcagga ggcacgagcc ggtggcggat
     9781 cccctcgagc tggaacatca ccaggaaggt gagaaacgcc gcaagctgga gcagataaat
     9841 gtactgcacc ggccatttgg tggcgaagac gaggggaatc ggcacggcga gcgaaagaag
     9901 cgctgcccat agcagcccga acgagcggta gccgtctgag gcaatcgccg cgacgaccac
     9961 gatctcgccg ctcgtcttct gctccgcgcg cgtgatcgcc gctgagatcg cggcgcgatc
    10021 ttcaggcgtg aagggctgca tcgaggaggg tgctaccatc cgcccgaggc cccgccgccg
    10081 ccgaaactgc cgccaccgcc ggaaaagccg ccaccgccac cgcctccccc gctccagccg
    10141 ccaccgccgc cgccatccca tgtcggtccc ggaagcaaga tcggaccggc agtgccactt
    10201 cgtcctgcgg gcgcggcgta gcgggcgctc cgccacatgc tgtaggcaat ccagaaaaag
    10261 atcgcgaccc agaggatgat gaagagccaa ccgatcatag gatcgtcggc gtctatccgc
    10321 gccctggcgc gcgcctcgac ttccgccgcg tccccggtca gcacgagaac gatgtcgcgg
    10381 acgccgttct tgatgccgcc gccgaaatcg ccctcgcgaa agcgcggcag gatcgaattc
    10441 tcgatgatga ttctcgacag cgcatcggtg agcgtcccct ctagtccggt gccgacttcg
    10501 atgcggacct tgcgctcgtt cggcgcgacg attaggagga cgccgttgtc gagtttctcg
    10561 gtgccgatgc cccagcggcg gccgagccga tagccgaagt cctcgatggc gtagccctga
    10621 agcgagggca gcgtgacgac cacgagctga tctgagctct tgtcctcgag cgccttgagg
    10681 tcggcggtaa cggcaacttt ttcggggtag gtgaggaggt tcgcgtcatc gacgacgcgg
    10741 ccggtcaact cgggaaagac cggctcggcc gcggcaggcg acataaggag cgcgagcgcc
    10801 gtggctatca gcatcccgcc gaagcgccgc acaattgcca ttggtgcccc ccattggctt
    10861 cgcgctagaa cttgacctcc gggggttttt ccgtgccttc cgcggcggtg aagttctgca
    10921 tcggctcggc gtcaggatag agcaaagcgc gccaccagcg gccggggatc gtcctgatct
    10981 cggtgttgta gtccctgacc gcggtaatgt agtcctgccg cgcgacggag atccggttct
    11041 cggtgccctc gagctgcgac tggagcgcga ggaagttctg gttggatttc aggtccggat
    11101 agttctcgac gatcagaagc aggcgcccga gcgcgcccgt gaggttgttc tgcgcgtcct
    11161 ggaatttctg gaacgcgtcg ggattggtga ggatgtcggg cggaatctgc acctgggtcg
    11221 ccttggcgcg ggcctcgacc acttcgatca ggaccttgct ctcctgcgcg gcataaccct
    11281 tcacggtctc gaccagattg gggatgagat cggcccggcg ctgatactgg ttcaacactt
    11341 ggctccacgc ggcctttgcc tgctcctgca aggtggggat ggtgttgatg ccacagccgg
    11401 cgagcacgac gcccgcaagg agcaggcagg caagccgaag catatggccc ggccgggcgt
    11461 gggtcagtgc cagcatgagt gatactcccc aatacaactc agtccgctag gcccatccct
    11521 gtattgagcg cgagcaggcg aagcaagagc tttcgccgcc gcttggaacc gcgattgcgg
    11581 agtggtgccg gggagcgaca ggccgttact tcggccgggc ggggccggaa tacgccgagt
    11641 tttggtgttg cgcgaatgaa acagcggcaa gttattcgcg atcggctcgc ccggcgcgcc
    11701 gccacatcgt gaacttaaaa tcgccaaaac ggggaggtaa gtaccagagg ctacgtgact
    11761 tttgttcacg gagcggcgcc cgcggcagaa ctttggcgcc gggtgcggcg ttaggttctt
    11821 ggaaggcttc cgctggtcaa atgagagtgc ctcttttagt gtgattcggc gcgaatcggg
    11881 cgtcggggga ctcacacggg ggcgcgctca tgggtgcggc cgatgactgg gagcttgtgt
    11941 cttgcgtatc gcgtttctga gggaaagggc ggctaaggcc gccaagcgaa cctcttcgac
    12001 acttttcgct ttcctgggcg ttgcggttgt ggccgccgcg gtcggcgcat cgatgccggc
    12061 cgaagccaag aagcagaaga acggcaacaa gcaggtcgag gcggcgatcc cggaccccgc
    12121 agacggcgag ccgatgacgc tcgtggtctc cctcagccag cagaaggtcg acgtctatcg
    12181 cggcgtgacc ctgatcacga gctccaaggt ttcgacggga atgcgcggct acgccaccaa
    12241 ggccggcgtg ttcagcatcc tcgaaaagcg gagacatcat cactccaacc tctatagcgg
    12301 cgcgccgatg ccgtggatga accggatcac ctggtccggc acggcgctgc atgcgggcgt
    12361 cgtgcccggc tatcccgcct cgcatggctg catccgcctg ccgttctcct tcgcgcccaa
    12421 gctgttcaag atcacgacgg tcggcgacaa cgtgatcgtg gcccgcgacc gcccggtgcc
    12481 gcaattgatc gcgcacgcca atctgttcca gccgctgcgc acgccggcgg cacctgcggt
    12541 agtcaagcag gaggaacccc cgaagcggca gtcgagcaac gaggcggtgc cgatggcgcc
    12601 ggtgaacact tatccaggca agctcgcaag ggccgacgtg ccgggcacca ccacagacgt
    12661 tccctcttcg ctcgaggccg aggcgatcgc gaccaagacc gcctcgccca acaacgccga
    12721 ccccgcagcg atccaggaca cgcacacgca tgcgttcgat ccgttcgctg gcgccgagcc
    12781 caaggcgagc gctcgcgatg gacacgcaac ggacaggagc gcgaaagcga gcggacatgc
    12841 gctcgacgaa gacgccggtt cggggcgttc acttgccaag gtcaaggcgg tgccggtcaa
    12901 accattgccc gcgatcgaga tcgcagccgc gcaagccgcg cctgctgccg ccgctcccgc
    12961 cgaagcgctc aagcctgtcg agcccgtggt atcgacactt gctccagccg cgcagccccc
    13021 gaagccggcc gagagtggga ctgcttccgc cgttccggcc gaggcggtct ccccagccga
    13081 gccgacgccg gccgatgctg cgagtgcggc gaacaaggtc gaggccgctg ggatgacggc
    13141 gggacccgcg gcggtcgaac aggcacgtgt cacagcggaa ccggcgcccg cttcgacccc
    13201 gactgcgccc gctgccggtc aggcgttggc cttggccaca cctccccaag cgagcccggc
    13261 cgaggcgagc gctctgccgc aactcgacgc gccgccgtcg gccgtcgccg caaggctcat
    13321 ggccggcgcc aaggaggcgg cgttgctggc cgcagagccg cgttcgacgg cgccgttgcg
    13381 catcctcgtg acgcggcgaa cccagcgcga ccgcatcatc ggcgttcagc gcatcttcgc
    13441 cgacatgggt tatctcgagc agcaggagtt cgacggcacc cttggcaagg cgacggtcgg
    13501 cgccatcaag gcttttcaaa aggcgaacgg catgccagag accggcgcgc tcaacgaaga
    13561 gctggcgaag aaggtctacg aggtgtccgg caagggcgag ccgccggcgg ggcatctcta
    13621 tgtcaggcag gaattcggcc gcgtgttcga tgcgcccgtc acgttcaagg accccgagac
    13681 gccgctcggc acgcatatct acacggccct gaaattcggg ccaggcgaca gctcggtgcg
    13741 ttggacgtcg atgagccttc agggcaacga ttcggcggcc gcgctcgaca ggctcgacat
    13801 cccgagcgac gttcgccaga agatctccga gcggctcacg cccggttctt cgctcatcat
    13861 cggcgacacg gccatcaaca cggcgggctt gaccaaaggc aacgacttcg tcgtttgggc
    13921 caaggagacg ccggccaaga tcaccgcggc gagcctcgac ggcgatttca cgccggccaa
    13981 gccgcggaag aagaaaaggg tgagggcggt gcgccggccc tatttcgatt acggctatga
    14041 ccgctatcag cgccccccgg gcttctcgcg cggccccgcc tggccctggt agggggctct
    14101 ggcgcgctcg cgcctcgttc ttgtcggcgc ccgactctgg cctcaccgcg atatgaaagc
    14161 ggcccctccg gggccgtttg ctttttgaat tgttgtgaaa ttggagcggg cgaggggatt
    14221 cgaacccccg accccaacct tggcaaggtt gtgctctacc cctgagctac acccgcgaac
    14281 tccaaccgaa aaacggccct tacgatctag ttgatcgccg ctggaatgtc cacctccggc
    14341 cggaaagggg ctagacccga atgaatccga aggacaggcc ccgacatcca agccattctt
    14401 gagcgcgcac gcgcgcaccg ccagacctta gacatgccag cagaccgcaa gacactcgac
    14461 gcccgccttg ccgcactcgg tatcgcaacc acgaccgtcg agcatgagcc ggtgttcacc
    14521 gtcgaggaat cggccgcttt gcggatgagc atccccggcg cccacaccaa gaacctgttc
    14581 ctcaccgacc ccgacggccg catggtcctc gtcacagcca aggacgacac gcgggtcgac
    14641 ctgaaggcgc ttgccaagcg gcttggcgcc gagcgcttca gtttcggcaa ggctccccgg
    14701 ctcgaggccg tgctcggcgt gagcgccgga tcggtgaccc cgctcgcgct catcaatgcg
    14761 tcggcggcag gcgtcaccgt cgtggtcgac caggcgctct tggactacga agaggtcaac
    14821 tgccacccgc tggaaaatac cgcgaccatg cgccttgcca cggccgatct catccgtttc
    14881 atcaaggaat gcggccagag ccgctgatct tctcgctgag ctgaggcgtc tcgaacagtt
    14941 gccaaatgcc gccgcgcagc ccatctttcg aggtaagggc ggctgatttc gtaggtaccg
    15001 cccgcgggca agcagcatga gcgtcgaaac tccgatcctg aagcaaggcg gtgcgggaac
    15061 ccccgacgcc gaactgatca agaacaccac taccaaggat ttcatgcgcg atgtcgtcga
    15121 ggcctcgcgc gaggtgccgg tgctggtcga tttctgggcg ccctggtgcg ggccctgccg
    15181 ccagctcacg ccgatcctgg agaaggccgt gcgcgcggcg aagggcgcga tcaagctggt
    15241 caagctcaat atcgacgagc acccgcaaat ccccggccaa atgggcgtgc aatccattcc
    15301 cgccgtgttc gcgttccagg acggcaagcc ggtcgacggt ttcatggggg ctctgccgga
    15361 gtcgcgcgtc aacgccttta tttcccggct cgtcggcgac tcggttgccg atgcggcggc
    15421 cgatctcgaa gttgcggagg cggcgcttgc cgccggcgat ctcaataccg cggcccagac
    15481 attcggcgag atcctgcaag cggaccgcga gaacgcgcaa gccctcgcag gcctcgccaa
    15541 gtgctacatc aggaccggcg acctcgcccg cgccgagcag acacttgcgc tggtgccgcc
    15601 ggccaaggcc gagagcgcgc cggtcgcgag cgcgcgcgcg gcgctcgagc tgcagcgcaa
    15661 atccgcaagc gtcgacgata tcgacaagct ccgcgccgcg gtcgcggcag atcccaacga
    15721 cgcgcaaagc cgcttcgacc tcgctctggc gctgaacgcc aagggcgaac gcgccgcagc
    15781 tctcgaagag ctgctcgcga tcatcgccaa gaaccgcgcc tggaacgacg acgccgcgcg
    15841 caagcagctg ttgcaactgt tcgacgcctg gggcgccgcc gatcccgcca cggtcgcggg
    15901 ccggcagagg ctctcctcgc tgctcttcgc ctgacgcaag gagcaaatcc gcgtgggtat
    15961 caccgaccgc tatcttaccg tcgcagacat gccgcagatg ctggcgatgt tccccttgcg
    16021 cggcgccatt ctgctcccgc gcgcctcgct cacgctcaac gtgttcgagc cgcgctatct
    16081 cgcgctcgtc gatcatgcgc ttgccgccga ccgcctgatc ggcgtggtgc agcccgcgcc
    16141 ggaagccgga gacgccgagt cgccgaaggg gcggagtttc cccttgcggt ccgtgggctg
    16201 cgcggggcgg ctcttctcct tcaccgaaag cgacgacggc cgcgtcgtga tcgcgctcgc
    16261 gggcatcgcg cgcttcacca tcaccggcga tgtcgcgggc gacacgccgt tccgcatctg
    16321 tcaggtcgat ttttcgccct tcgccaagga cttcgtcacc ggccacggcg aggacgacgt
    16381 cgaccgcccg cgccttctcg ccacgctcaa aaactatctc accgcgagca acctcaacgc
    16441 ggactgggac cgcatcgacg gcgcctccag cgagcggctg gtcaatacgc tgtcgatcct
    16501 cagtccctat gggccggagg agaagcaggc tctgctcgaa gcgcccgact tgcgcgcgcg
    16561 cgccgaggca ctggtcgcgc tcgcagagat ggagctcgcg gctcgggacg acggttcggg
    16621 gacttcgata caatgacgga ggcgcggccg tgaccaacga acgcggcggc gaacgccgcc
    16681 tcgatccgaa gctcctcgag atcctggtct gtcccctgac caagacctcg ctcgactacg
    16741 accccgaacg ccaggagctg atcagccgcg ccgcgggcct cgcctatccg atccgcgacg
    16801 gcatcccgat catgctgccg gaagaagcgc gcaagctcga ccagagctag aacctttgcc
    16861 ccgtcagcag gctgggcacc gtcccggacc gtcccgccgc ttcgctgacg aggaagcgct
    16921 tcagcgcggg agcccgatcg accagcccca ggcccaggtc gcgcagcgtg cgcaaggggg
    16981 cgctgtcgtt cgagaacagc cggttcaatc catccatcac ggcggccgag aaggcgctgt
    17041 cgaaacggcg ccagcgcacg tagcgctcaa gcgggactgc gctgccgatg tcgaggccga
    17101 gccgtgccgt ttcgaccacg gtctccgtga gcgcggcgac gtcgcgcatg ccgatattga
    17161 gcccctgccc cgccagcgga tgaacgacgt gcgccgcgtc gccgatgagc gcaaagcggt
    17221 ctgccacgaa agagcgggcg atctgcatgt cgaggctgaa ggactgccgg agaccggcaa
    17281 gagagatcgc gccgagcctg tgtccgaagc gcttggtgag ctcttcgagg aagcgggcct
    17341 caccggccgc catgatggcg gcgccttctg cggcgtcctc ggtccacacg atggacgagc
    17401 ggttcccggt aagagggagg atggcgaacg gccccgcggg caggaaatgc tgcacggcct
    17461 tgccgtgatg cggccgctca tgcgcgaccg tggtcacgat gcccacttgc ggataggacc
    17521 agcccacgca tttgatgccg gcgcgctcgc gcagatgcga gcgcttgcca tcggcggcga
    17581 cgagaaggct cgcagcgatc gtcatgccac tggcaagcga gacctcggcg gcaaaaggac
    17641 cggcgttgaa atcggtcacg cgatcggaag gataaacagc gacggcgggc tcgaccgcaa
    17701 cagcttcggc cagcacgcgg tcaagatcgc cgtgctccgc catgaaggcg ctcgtgccgc
    17761 tctctccaag ctcctcgtcg aagccgagca aatggggacg gagcgctgca tcgagcgcgc
    17821 tgtcggtaat ctcgatcgct tcgatagctt ggactgacgg cgcaagctct gcccagagat
    17881 cgagcgccga aagcagattc ttggttgccg ccgagagcgc gagcgagcga gcgtcttcca
    17941 tgccgcttcg tccggtctcc gccagctccg cgtcgatcaa ggcgatgcgg aaaccttgcg
    18001 gcgcaaggcg gccgagcgcg agcgcgagcg tccggcccaa gagcccgccg cccgcgaccg
    18061 caacgtcgaa ccgttccagg cctcgtgtgc gtccatccgg catgggagag ccatgacacg
    18121 ttccgcagct tgcgtgaacc tgctaaaggc ttctcccgcc cgccttggaa ggacttcgcc
    18181 cgtgagccgc gcgctggaac agcttctttc gatcctcgag ctcgagcagc tcgaagtgaa
    18241 cctgtttcga ggcttgagcc ctcaggtcgg ctggcagcgc gtgttcggcg gacaggtgat
    18301 cggccaggcc ctggtcgcgg cccagcgcac ggtggttgac cgcctcgccc actccctgca
    18361 cgcctatttc ttgcggcccg gcgatcccgc ggtgccgatc atctataacg tcgaccgcat
    18421 ccgcgacggc ggcagcttca caacacgccg cgtggtggcg atccagcacg gccacgcgat
    18481 cttctccatg gccgcctcgt tccacaagga ggagcccgga ctccagcacc aaaccagcat
    18541 gccggacgtg ccgccgccgg aagcgctgcc cagcgaggcg gagctcaagg cgaaactcat
    18601 cgacaagctg ccgccgcagg tcaaaatcta ttgggagcgc gagcggccga tcgaaatcag
    18661 gccggtcgat ctatcgcgct atctttcgcc gcagaaccgt gcggcgagcc agcaggtctg
    18721 gatcagggcg acgggcaagc tcggcgacga tctcgcgcta catcaatgcg tgctggccta
    18781 tgcgtcggat tttacgctgc tcgacacggc gctgatcgcg catggccgct tcattttcga
    18841 tccgcggctg atgctcgcga gcctcgacca cgcgctctgg ttccatcgcc gcttcaggtc
    18901 ggatgactgg ctgctctacg cccaggacag tccaagctcg ggcgccgggc gggcgttctg
    18961 ccgcggcacc ttgttcacgc gcgacggggt gcttgtggcc tcgaccgcgc aggaaggatt
    19021 gctccgcgaa cgccagcccg ggagttgatg cctattccat aggcatcggc tctctttagc
    19081 tcatatttta ggctgaccgg agaacctccc gtgaggccgg gcccgaccgc ttctcgaaaa
    19141 acctaaacaa gatcagctat ttacaaaatc agccgcgagt tggcacgcgg cttgactctc
    19201 ctcatccgtt cgcgcgaccg gacgggcgtt cgagacccgg tcggccgcaa tcatggaggg
    19261 gtgagagatg aagctcgtga tggcgatcat caagccattc aagctcgatg aggtgcgcca
    19321 agccctcaca gagctcggac tgcaaggcat gaccgtgacc gaagtgaaag gctacgggcg
    19381 gcagaagggc catacggaga tctatcgcgg cgccgaatac gccgtgaact tcctgcccaa
    19441 gatcaagatc gaggtcgtga tcccgaccaa gatggtcgac aaggccgtgg agggcatcgt
    19501 cgcggcggca aagaccggac agatcggcga cggcaagatc ttcgtcgtcc ccgtcgaaca
    19561 gacggtgcgc atccgcaccg gcgaaaccaa cgaatcagct ctgtaagagg ggagatgaag
    19621 atgacccttg ccaaatatct ttcttcggcg gttctcgcgc tcgccgcgct cctgatcgcc
    19681 aagcttgatc ccgcgcttgc gcaagaggcg gccgaggcgg ccgccgaagc accgaagccg
    19741 gccatcgaca gcggcgatac ggcgtggatg ctcacatcca cggcgctcgt gctaatgatg
    19801 accgtgccgg gtcttgcgct cttctatggc ggcatggtgc gcaagaagaa tgtgctcagc
    19861 atcatcatgc aatgctttgc cacgacctgc atcgtcaccg tggcgtggat ggtgctcggc
    19921 tactcttggg ccttcaccga cggtggcggc atgcagagct atctcggcgg catgtcgcaa
    19981 ttcatgctga agagcatgac gatcgacagc acaaattcgc tcgcggcgac gatccccgag
    20041 tcggtgttca tgacgttcca gatgaccttc gccatcatca cgcccgcgct catcgtcggc
    20101 gcctttgccg accgcatgaa gttctcggcg ctcgtgctgt tcatgacgct gtgggtgctg
    20161 ttcgtctatg ccccgatcgc gcattgggtg tggggcggcg gctttctcgg cggcgcgggc
    20221 gtgctcgact tcgccggcgg aaccgtcgtg catatcaatg cgggcgtcgc cggtctcgtc
    20281 accgcgctct atctcggcaa gcgcagcggt tacgggtccg agaacatggc cccgcacaac
    20341 ctcgtctaca gcctgaccgg agccgcgctg ctgtgggtcg gctggttcgg cttcaacgcc
    20401 ggatcggcgg tggccgccaa tggacgcgcg ggcatggcga tggcggtcac ccagattgcc
    20461 acggcaacgg cggcgctcgg ctggatgttc gccgaatggc tcgtgcaccg gaagccgagc
    20521 gtgctcggca tcatttcggg ggcggtcgcg ggcctcgtgg ccattacccc ggcctcgggc
    20581 ttcgtcggtc cggtcggcgc gctgtggatc ggcgcggcgg cgggcgtggg ctgcttcttt
    20641 gccgcgacca cggtcaagcg cgccatcggc tacgacgatt cgctcgacgt tttcggggtg
    20701 cacggcatcg gcggcatcat cggggcgatc ctgaccggcg tgttcgccgt tgaggcgatc
    20761 ggcggcgcca aaggcgctct cgaaggcaat gtcggccaga tctggacgca gatcgaaggc
    20821 attgccgcga ccatcgcgtg gtcgggactg gccaccttgg tcattctcgt cgtggtgaac
    20881 atcttgaccg gcgtccgcgt gagccaggcc gtcgaggtcg aaggtctcga catcaacctg
    20941 catggggagg tggtaccgta aggtctcctc gcgccaaacg accgaaccga acgaaaagag
    21001 agccaagtcg ccccttcccg cgacttggct ctcgcccttt gaagccgggc atctcacggc
    21061 cattgccgca agcctcgcca tcccctaaca ccggccgcat gtctccccgc gacatgccga
    21121 cttacctcga cgacgtggtg cgctcgccat ttgtccgact cgcggctctg ctcgaaggga
    21181 tttcgccggg cgcgcccccg atcgacctct cgcttggaga acccaaggcg ctgatcccgc
    21241 ctttccttgg cccgacgctg caaaaccatc tcggcgaatt cggccgctat ccgccgatca
    21301 aggggatccc ttcgctccgg aacgcgatcg ccgattggat ggggcggcgc tatccgatcc
    21361 tgaagggcgc gatcgacccc gaacagcaca tcctgccgct caacggatcg cgcgagggat
    21421 tgttctcggc catctttccc gcaaaggcgc ggaagcctca gctcgacgaa ccggcggtcc
    21481 tgatcccgaa ccccttctac caggcctatg ccgcggccgc cgcggcctcg ggcgccccac
    21541 ctgtgttcct cacagcgcga gccgaagatg gcttcctgcc aaggctcgac gacattgacg
    21601 cggctctcct tcagcgcacg gccgccttct atctcaacac cccgtcgaac ccgcaaggcg
    21661 ccgtcgccga ctgctcctat cttgcgaagg cgaccgcgct cgcgcgacga ttcgattttc
    21721 tcctcttcgc cgacgagtgc tattccgaga tctatggcga gacccctcct ccgggagcgc
    21781 tcgagacagc ttgggccgag acgcggagcc tctccaatgt cgtgtcgttc cagtcgctgt
    21841 cgaagcgctc gggcctgccg ggcctccggt cggggttcgt cgcaggcgac cccgtcttca
    21901 gtgccgcctt cgcccgcttt cgcaacgtcg cctgcccgca agtgccgctt cccgtgcagc
    21961 atgtctccgc cgaggcttgg gcggacgagc gccatgtcga ggaggggcgg gcgttgtacc
    22021 ggcggagttt cgccatcgcc gacgagcttc tcgacggccg gtatggctac cggcgcccgc
    22081 aaggaagctt ctttctttgg ctgaaaatgg ctgttttcgg gggcggggag gcggccgcga
    22141 aaaccctttg gaaagggtgt ggtgtcagag tgctgccggg cacttatctc gcacaggccg
    22201 gacccgatgg cgtcaatccg ggtatcgacc atgtcaggat cgcgctcgtg catgacgcgg
    22261 agaccacacg cgaggccttg acgcgggtcg tcgccacatt gggttgaggg gatggccagc
    22321 agaggaagat cgaaggcgcg ccggcgcctc ctgccgggag cggtcgaaac cggcctgcgc
    22381 cggatcatgt tcggcggcta cgggttgctg ttcgtcacga tcgcctgtgc cgcatgggcg
    22441 agcctcgcca cctggtcggt gcacgaccct agcctcaaca acgccacgcg cgcggtgccg
    22501 cgcaattttc tcggctcgtg gggcgcggtg ctcgccgacc tcaccatcca atcccttggc
    22561 ctcgccgcga tcattctgtt cctgccactt gcggcatggg ggtggcacct cgtcttcagc
    22621 acgacgccgg accgcagaag acggcggttc ctcgcctggc ccgcatccgt gatcctgctt
    22681 gccgcggcgt tcgcggcttt cccgcaaccc aaaagctggc ccctccccaa tggtctcggc
    22741 ggcattctcg gcgacttctt catggccggc gcccacgtca ttgggccttt cctgcccgag
    22801 gccgcggtgt cctttgtcgc cgggctcgcc ttcttcgtga tcggcacgat gctgctcctg
    22861 tttgcttgcg gcacctcgac ctcgcggctc attgcgctgt gggcgccgca gagcggcgtt
    22921 gccggcgaat gggccaatgc ctcgctcggc gcggcgatgc atgtcgccat gcatgccggc
    22981 gcgagactgc ggcacacctt ccgcgggcgc gacgcgttca acgaggagat tgaggatcgg
    23041 ctcgatgagg gcgcggacga cgaggacgtg gagcccgcgc gcaggctcga cgccgatgga
    23101 cgcatcgaac cctcgttcgg cccggcgccg atcggcgaag cgttcgacga ggacgagggg
    23161 gacgacgaca tcacgtcgcc caactaccgc atcacgcgcc tcaatccgca aaagaaaagg
    23221 aagcataccg gcctcgcccg ccagacccac tccgacgaac cttacgagcc gccctcgatc
    23281 aagctcctgc aaccgccgaa agggagcaag ggacgcggcg tcagcgacga ggtgttgcag
    23341 gagaacgcgc gcgaactgga gggcgtgctt caggacttcg gcgtcaaggg cgagatcacc
    23401 aatgtccggc cgggcccggt ggtcacgctc tacgagctcg agcccgcgcc ggggaccaag
    23461 tcgtcgcgcg tcatcgggct tgccgacgat atcgcgcgct cgatgagcgc ggtcgcggcg
    23521 cgcgtcgcca tcgtctccgg gcgcaacgcc atcggcatcg agctccccaa cgaccgccgc
    23581 gagatcgtgg tcttgcgcga gatgatcgaa tcgccggact tcgaggagac cgacgcgagg
    23641 ctcgcgcttg cgctcggcaa gaatatcggc ggcgaggcga tcatcgtcga tctcgctcgc
    23701 atgccgcatc tgctcatcgc cggcaccacc ggctccggca agtcggtcgg catcaacacc
    23761 atgatcctgt cgctgctcta ccggctgtcg cccgaccaat gtaagttcat catgatcgat
    23821 ccaaagatgc tcgagctgtc cgtctatgac ggcatcccgc atctgctcgc gcccgttgtc
    23881 accgatccga agaaggcggt ggtcgcgctc aaatggacgg tgcgcgagat ggaggagcgc
    23941 tacaagcgca tgtcgaaatt gggcgtgcgc gacatcaagg gctacaacag ccgcatcctg
    24001 caagccgagg ccaagggtga ggtgctgacc cgcacggtgc agaccggctt cgaccgcgcg
    24061 accggccgcg caatctacga gcaggaggag atggactacg acgccatgcc cttcatcgtc
    24121 gtcatcgtcg acgagatggc cgacctgatg atggtcgccg gcaaggacat cgaagcagcc
    24181 gtgcagcgtc tcgcgcagat ggcgcgcgcc gccggcatcc acctcatcac cgccactcag
    24241 cgcccgagcg tcgacgtgat caccggcacc atcaaggcga atttcccgac gcgcgtcagc
    24301 ttccaggtca cctcgaagat cgacagccgc accattctcg gcgagcaggg ggccgagcag
    24361 ctcctcggcc agggcgacat gctgttcatg gccgggggcg gacgcatcca gcgcgtccac
    24421 ggcccctacg tcgccgatac cgaggtcgag gagatcgtcg cg
//
LOCUS       DASUQP010000185         4972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1453673, whole genome shotgun sequence.
ACCESSION   DASUQP010000185 DASUQP010000000
VERSION     DASUQP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453673"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..497
                     /locus_tag="VFY74_08315"
     CDS             174..497
                     /locus_tag="VFY74_08315"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5931733.1"
                     /translation="MTHRATLALVLLAVGVSPALATSGPGCLVVVNVAAADVLNLRAS
                     PSPSARIVDTLVPGQHGIIHLDAQCRPKALPWGSRWCPVTHYQGDKVAQGWVKARYVR
                     DSDCP"
     gene            complement(540..2723)
                     /locus_tag="VFY74_08320"
     CDS             complement(540..2723)
                     /locus_tag="VFY74_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5931734.1"
                     /translation="MTGRDGNLCSHCLLPIGIRPMQRTVNGEDCSFCCYGCCIAYQVK
                     HGQSEEWEAAWLLIRLGVGAFLTMNIMLISLLLYAGTFSADPWLIPWIHLLLWLFATP
                     ALIILGEPYLREMWTAAAQGRVTSSALVVLGVTAAYAYSVFATVEGSEEVYFDTATMV
                     LMLFTVGAYIEAAGRAKAARDLEPLLAAESESATTVDGGVEVRRPVRDIAPGMLVRVR
                     PGERIPVDGVVVEGRSHADESVMTGESRQVVKSAGSTVIAGSMNLDGPLLLRSSGAGS
                     ATRWAQICRSVREALSRRSPIQRIADRLVGLSVPLVLLLGGLAVGYWAQTLPFDRALL
                     IGLAVLVVACPCAVGLAAPLATSLGIGRLARCGCLVRDPGTLEALARTRLIAFDKTGT
                     LTSGTSRLVGIATEGATTDLVLARAAGLERYSEHGLARAIIATAEAKGIAPDVAGDVQ
                     AVPGRGIKGQVGGEPVAAGNRAFMRDLGWPLPPLLAEKARSLEASGHSVISIGWGGRV
                     HAVLAFDDTPHPEARATIEELRRLGLQVALLTGDLAPAARRIAAMVGIDDVQAELSPE
                     AKQAALDEYRQRHEVVAMVGDGLNDGPVLADADVGIAVGSATDLARETAALVLPKDGL
                     CMLPWVIVVSRAVRLTIVTNLAWAFGYNLIALTLAALGTLQPVLAAAVMASSSLLLVV
                     NSLRLSRLPDPALPGIDRRAPGIEPADIRRNLGVPAMASPATEQR"
     gene            complement(2720..3442)
                     /locus_tag="VFY74_08325"
     CDS             complement(2720..3442)
                     /locus_tag="VFY74_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5931735.1"
                     /translation="MTYYLVIFVAGFAGSFHCIGMCGGFACALGRDRGGAAATAGRHV
                     LYNFGRLTTYCFLGAAAGALGEMLCTSRGLAVPVLNGPLDGAQRGLAIVAGLLMIAVA
                     LQFFGVLQRFQGATIGFGGSTLAMSLRNLLTSENRAAPVAFGVFNGFLPCPLVYAFAV
                     QAASTASAVSGFLVMLAFGLGTFPAMLMMGGVGRLLAPIWRQRGVWVAGSFILLLGLI
                     TLGRGVLPFDTHLHALPQGSPG"
     gene            complement(3502..3624)
                     /locus_tag="VFY74_08330"
     CDS             complement(3502..3624)
                     /locus_tag="VFY74_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931736.1"
                     /translation="MLEHTAFTFANADIYQMIAYIGGAIAHTAFFVWVIWLIRW"
     gene            complement(3751..3996)
                     /locus_tag="VFY74_08335"
     CDS             complement(3751..3996)
                     /locus_tag="VFY74_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931737.1"
                     /translation="MTQTDNTPTKPRSEPHSHADELEEYGGGAIEGRHGYIPLWLLAV
                     YAVLFIWGLYYAYAYWGGLGPGRIYLSEPPESREVID"
     gene            complement(3956..4144)
                     /locus_tag="VFY74_08340"
     CDS             complement(3956..4144)
                     /locus_tag="VFY74_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931738.1"
                     /translation="MEGFEITGPYLSALLMSLGAVCVFVWGVLSGAFHRADEASVRFC
                     EMEMESDANRQHADQAKE"
     gene            complement(4148..>4972)
                     /locus_tag="VFY74_08345"
     CDS             complement(4148..>4972)
                     /locus_tag="VFY74_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5931739.1"
                     /translation="VYERRCLGCHGEKGDGNGPASTFFYLQRPRNFNNAVFKFRLTNE
                     AVPADGDLFRTITRGVRGTAMPAWYELPVNDRLAVIQYIKYVLAVDRSDPAKPYAFFL
                     EEPPGAPLYIGKPPAPSQELIARGKEVWDQAKCWECHGQGGKGDGEKAAGLEDDDGFT
                     IVPADLTAGQFKSGPTVQDIFRTMTTGLTGTPMPSYGDSLPEEDRWALSYYVLALSAY
                     KDPLTKQPLAIPEAARAALNNPDLTADTPETAYIPGQDAQPKAAAMGATQAATTGE"
BASE COUNT          956 a         1629 c         1597 g          790 t
ORIGIN      
        1 cagccgtctc gcggctatga atgatcttat aggcctgcgg tacgaacgcc gcggtggtca
       61 gcgtcgctgc aattccagac acgatcgtct ccatcgtcag tccttcacgc caaaatcctg
      121 atctagcagc ctttgcaaag atagggcatc gatttcatcc aaggtgatac ggcatgacgc
      181 atagagcgac cctcgccctt gtccttcttg cggttggcgt gtcaccggcg ctcgcgacat
      241 cgggtcccgg ctgcctggtt gtcgtgaacg tcgccgccgc cgacgtcctc aatcttcgcg
      301 catcgccatc gccatcggcg cggatcgtgg acacgctcgt ccccggccag cacggcatca
      361 tccatctcga cgcgcagtgc cggcccaagg cgctgccttg gggcagtcgc tggtgcccgg
      421 tgacgcacta tcagggcgac aaggttgcgc agggttgggt caaggctcgt tacgtgcggg
      481 acagtgactg cccgtgatat tcctatcaat taagatgcga agcttcctgt tgcttgcgat
      541 cagcgctgtt cagttgcggg ggaggccatc gccggcacgc cgaggttgcg gcgaatatcg
      601 gcgggctcaa tgcccggcgc gcgccgatcg atgcccggta gcgccggatc gggaagccgt
      661 gacagccgca gcgaattcac cacgagcagc aggctcgaac tcgccatcac ggcggcggcc
      721 agcaccggct gcaacgttcc aagcgccgca agcgtaagcg cgatgagatt gtagccaaag
      781 gcccaggcga ggttggtgac gatggtcaag cgcaccgcgc gcgacaccac gatcacccaa
      841 ggcaacatgc acaaaccgtc cttgggcagg acgagcgcgg ccgtctcccg cgcgaggtct
      901 gtcgccgagc cgacggcaat gccgacgtca gcgtcggcaa gcacgggccc gtcgttcagg
      961 ccatccccga ccatggccac cacttcgtgg cgttgccgat attcatcgag cgcggcctgc
     1021 ttcgcttccg gcgaaagttc cgcttgcacg tcgtcgatcc cgaccatggc ggcaatcctg
     1081 cgggcggccg gcgcgagatc gccggtcagg agcgcaactt gaagcccgag gcggcgcagt
     1141 tcctcgatcg tcgcccgcgc ctcgggatgg ggcgtgtcgt cgaaggccaa aacggcatgg
     1201 acgcggcccc cccagccgat ggagatgacg gaatgaccgc tcgcctccag cgaccgggcc
     1261 ttctcggcga ggagcggcgg gagcggccaa ccgagatcgc gcatgaaggc tcgatttccg
     1321 gcggcgaccg gctcgccgcc gacctgtccc ttgatgccgc ggccgggaac ggcttgaaca
     1381 tcaccggcga cgtcgggtgc tatgcctttc gcctcggctg tcgcgatgat ggctcgcgcg
     1441 agtccatgtt cggaatagcg ttcaagcccg gcggcgcggg caagcacgag gtcggtcgtc
     1501 gccccttcgg tcgcgatacc gacaaggcgc gatgtgcctg aggtcagggt accggtcttg
     1561 tcgaaggcga tcagccgcgt gcgggcaagc gcttccaaag tgcccggatc ccgcacgagg
     1621 cagccgcagc gcgcgagccg gccaattccg agcgaggtcg cgagcggcgc ggcgagaccg
     1681 accgcgcaag gacaggccac gaccagcacc gcaaggccaa tgaggagcgc acggtcgaac
     1741 ggcagtgtct gcgcccaata cccgacggca agcccgccga gaaggagaac gagcggcacc
     1801 gagaggccga cgaggcgatc ggcgatgcgc tggatcgggc ttcggcgcga cagggcctcg
     1861 cgaaccgagc ggcaaatctg cgcccagcgc gtggccgagc cggcgccgct gcttcggagc
     1921 aacaacggtc cgtccagatt catgctgccg gcgatcacgg tggagcccgc acttttgacc
     1981 acctggcggc tttctccggt catcaccgat tcatcggcgt gggagcgacc ctcgaccacg
     2041 acgccgtcga cgggaatgcg ctcgccgggc cggacacgca caagcatccc cggcgcgata
     2101 tcccgcaccg gccggcgcac ctcgacgcct ccatcgaccg tcgtcgcgga ctcgctttca
     2161 gccgccagga gcggctcaag atcgcgcgcc gccttggcgc ggcccgccgc ctcgatatag
     2221 gcgccgacgg taaacagcat cagcaccatg gttgccgtgt cgaaatagac ctcctcgctg
     2281 ccttcgacgg tcgcgaagac ggaataggcg taggcggcgg tgacgccgag aacgacgagc
     2341 gccgaggagg tgacgcgccc ctgtgccgcc gccgtccaca tttcgcgcaa gtacggctcg
     2401 ccgagaatga tcaatgccgg cgtcgcgaaa agccagagca gcaggtgaat ccacgggatc
     2461 aaccaggggt cggcgctgaa cgtgcctgcg tagagcaaga gactgatcag catgatgttc
     2521 atggtgagaa aggcgccgac gccgagcctg atcaaaagcc aggccgcctc ccactcttcg
     2581 ctctggccgt gcttcacctg ataggcgatg cagcacccat agcagcagaa ggaacagtcc
     2641 tcgccgttca ccgtgcgctg catcgggcgg atcccgatgg gcagcaggca atggctgcac
     2701 agattgccgt ctcgtccagt catcccggac ttccttgcgg caaggcatgc agatgggtgt
     2761 caaagggcaa cacgccgcgt ccaagtgtaa tgagcccgag caacaggatg aagctgccgg
     2821 ccacccacac gccgcgctgc cgccatatcg gcgcgagaag gcgcccaacc ccacccatca
     2881 tcaacatcgc aggaaacgta cccaagccga atgccagcat gacgagaaag ccggacacgg
     2941 cgctggcggt gctcgccgcc tggacggcga aggcatagac cagaggacag ggcagaaatc
     3001 cgttgaacac gccaaacgcg accggggcgg cacggttctc tgacgtcagc agattgcgca
     3061 aggacatcgc aagggtgctg ccgccaaagc cgatcgttgc accttggaag cgttggagca
     3121 cgccgaagaa ctgtaacgcg acggcgatca tgagaagtcc ggcgacaatc gccagaccgc
     3181 gctgggcgcc gtcgagcggg ccgtttagaa cggggaccgc caacccccgc gaggtgcaca
     3241 gcatctcgcc caacgcgcct gccgccgcac ccaggaaaca gtaagtggtg agccggccga
     3301 aattgtagag cacgtgccgg cccgcggtcg cggccgcccc gccccgatcg cggccgagcg
     3361 cgcaggcaaa gccgccgcac atgccgatgc aatgaaagct gccggcgaac ccggcgacga
     3421 atatcacgag ataataggtc actggcagag actacccgac cagtcgccca tccgtcaccg
     3481 agagagtgtt gcgcagcgcc gctaccagcg gatcagccag atgacccaaa cgaaaaaggc
     3541 ggtgtgggcg attgcgccgc cgatataggc gatcatttgg tagatatccg cgtttgcgaa
     3601 cgtgaaagcc gtatgctcga gcacgccccc ctcccgctca gcccaaggag accagtttca
     3661 cgttgaccgc cagagcggtg aacagcaaga tcaagttcaa tatcgcggcc gccaacacga
     3721 gcttcgctgc cagcatggtc tcttcctttc ctaatcgatg acttcccggc tttcaggtgg
     3781 ctccgataga tagatgcgcc cgggaccgag gccgccccaa taggcgtagg cgtaatagag
     3841 tccccagatg aagagcaccg cgtagacggc caagagccat agcgggatat agccgtgccg
     3901 cccctcgatg gcgccgccgc catattcctc gagctcgtcc gcgtgggagt gcggttcact
     3961 ccttggcttg gtcggcgtgt tgtctgtttg cgtcactttc catctccatc tcgcagaatc
     4021 tgacggacgc ctcgtcggcg cggtggaatg cgcccgaaag cacgccccaa acaaagacgc
     4081 agaccgcgcc aagactcatc aagagcgcgc tgaggtaggg tccagtgatc tcgaaaccct
     4141 ccatgtctca ttctcctgtg gtggcggctt gcgtggcgcc catggccgca gccttcggct
     4201 gcgcgtcctg gccaggaatg taggccgtct caggggtatc ggctgtgaga tcggggttgt
     4261 tcagcgccgc ccgggccgcc tcggggatcg ccagcggctg cttcgtcagc ggatccttgt
     4321 aggcggatag ggcgagaaca tagtaggaga gcgcccaccg atcctcctcg gggagcgagt
     4381 cgccgtaaga cggcatgggc gtgccggtca atccggtcgt catggtgcgg aagatgtcct
     4441 gaaccgtcgg cccggacttg aattgacccg ccgtcaggtc ggccgggacg atcgtgaaac
     4501 cgtcgtcatc ctcgagcccg gcagctttct cgccgtcgcc cttcccgcct tgaccgtggc
     4561 attcccagca tttcgcttgg tcccaaactt ccttgccccg agcgatgagc tcttgcgagg
     4621 gagcgggcgg cttgccgata tacaagggcg cgccgggcgg ctcctccagg aagaaagcgt
     4681 agggctttgc gggatcggag cggtccaccg ccagcacata tttgatgtac tggatcacgg
     4741 cgaggcggtc gttgaccggc agctcatacc aggcaggcat cgccgtaccg cggacgccgc
     4801 gcgtgatggt ccggaacaga tcgccgtcgg ccggcactgc ctcgttggtc aacctgaact
     4861 tgaagacggc gttgttgaag ttccgcggac gctgaaggta gaagaaggtc gatgcagggc
     4921 cgttgccgtc gcctttctcg ccatggcagc cgaggcaccg gcgctcatag ac
//
LOCUS       DASUQP010000186         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_637639,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000186 DASUQP010000000
VERSION     DASUQP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637639"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1429)
                     /locus_tag="VFY74_08350"
     CDS             complement(113..1429)
                     /locus_tag="VFY74_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931740.1"
                     /translation="MEEIHRRCGVYTRREVVIRLLDSIGWKADANLAEARLLEPATGD
                     GMFIGEATSRLLKSLSRGKRRPNKKQLIDRIVAFEIHPVEARRARKRVREVLKSCGMK
                     PADAATIARSWVRTTDFLLAEIAEESFSHVAGNPPYARWSKIPPKLRSQYEVRLPQAV
                     ARGDLFLPFLDLSIGYLRKGGRLGFVCSDRWRYMAFADGFRTTRLPEVVIEKETPVEA
                     DTAYQRWVDAYPSLLVMRKRQRAGKRKSALRPSRRMTLVEAGYRVRVGPALGCTRAFV
                     LDPEDDRVEPELTLLWLDASEVGEGEIRWSGRQVIAMNAPDGKLRDLGNFPLAKARLE
                     RFRLELEKRSIVRAGATWYRPIDRIRASDWSRPKLLIPELAKIPRVAPDTSGAIPSHG
                     VYAIFAPNNDLEALLARLANGGLARLSILRPRDLSTRSLVALSRLS"
     gene            complement(1560..1697)
                     /locus_tag="VFY74_08355"
     CDS             complement(1560..1697)
                     /locus_tag="VFY74_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931741.1"
                     /translation="MPEDRLRRGVDLGQHDSVVPGLGKGEFEASDSCEQTRDRHGVEA
                     C"
     gene            2409..3593
                     /locus_tag="VFY74_08360"
     CDS             2409..3593
                     /locus_tag="VFY74_08360"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX5931742.1"
                     /translation="MIGSEPRYQAFLESRAREGLRRFLLAVAAGEARTLAAEGGSYVN
                     FSSNDYLGLRFNAALIERAMTWAREWGVGSGASRLVTGNLDLFRPIEAKLARLKGKQA
                     ALVMASGFQTNAAALQALLDRSVLGAEPLVFADRLNHASMHFGCEAAGARQIRYRHGD
                     AGHLGELLDRHQADDRPKFILTESVFSMDGDVAPLGDIARLARAHAASLIVDDAHATG
                     ILGEAGSGLSGEADIVIGTFSKALGSFGGFVASSEKVRDYLINRCAGVIYSTALPPPV
                     LGAIDAALDIVPGMDAERAYVAKLAERFRTGARASGLDIGRSTTQIVPILVGQPEAAL
                     TLSGRLREAGLWATSIRPPAVPPGTARLRLAFTAAHQDADVDRLLEVLSAKDAPRRAR
                     AV"
     gene            3603..>3902
                     /locus_tag="VFY74_08365"
     CDS             3603..>3902
                     /locus_tag="VFY74_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931743.1"
                     /translation="MHVVLVHGWGFHAGLWTDFVRHLDGTGVTLVDLGFVSGGPKGER
                     DWPPDSIAVGHSLGLLWLLARGGGQFRALVSVQGFDCFCCHVALPRVAALKRGIER"
BASE COUNT          698 a         1233 c         1199 g          772 t
ORIGIN      
        1 cgcaccactc agccgcttag cctcgtgagc gtgggatggg agtgtcgtac aagccgagcc
       61 tgaaggaata ctgcagcata ctgaaggatt acgccatcag cggaatcgca tcttatgaca
      121 gccttgatag cgcgacgaga ctccgcgtgg agagatctct cggtcgcagg atcgagagcc
      181 gcgccaaccc cccatttgca agccgtgcca gcaatgcttc aagatcgttg ttgggcgcga
      241 aaatcgcata cacgccgtgt gacggaatcg ctccggatgt gtccggcgca acgcgcggga
      301 ttttggcgag ctcagggatc aggagtttcg ggcgcgacca gtccgatgcc cttattcggt
      361 ctatcggtct gtaccaagtt gcgcctgctc tgacgatcga acgcttctcc agctcaaggc
      421 ggaatctttc caggcgagcc ttggccaggg ggaagttccc aagatcgcga agcttgccgt
      481 ctggagcgtt cattgcgata acctgccggc cgctccatcg gatttctccc tcgccgacct
      541 cggaagcatc caaccacagt aacgtgagct ccggctcgac acgatcgtcc tcggggtcga
      601 gcacgaaagc tctggtgcag cctagcgcag ggccgacgcg gacgcgatac ccggcttcga
      661 cgagcgtcat ccttcgggac ggccgcaacg cggatttccg tttgcctgcc cgctgacgtt
      721 tcctcattac gagaagagaa ggataggcgt cgacccaccg ctgatatgct gtgtcggctt
      781 cgaccggcgt ctccttttcg atgacgactt cgggaagccg cgtagtccgg aacccatcag
      841 caaacgccat atagcgccag cgatcggaac acacgaaccc caaccgtccg cccttcctta
      901 aatagccaat ggacagatcg agaaacggca ggaagagatc gccgcgcgcc accgcctggg
      961 gcagtcgtac ttcgtattgg ctgcgcagtt tcggagggat cttcgaccat ctcgcataag
     1021 gaggattgcc ggccacgtga gagaacgact cctccgcgat ctcagcgagc agaaaatcgg
     1081 tcgtcctcac ccacgagcgc gcgatagtcg cagcatcggc tggtttcatg ccgcagctct
     1141 tgagaacctc gcgcactctc ttccgtgcgc gccgggcttc gacgggatga atttcgaacg
     1201 cgacgattct gtcgatcagt tgtttcttgt tgggtctgcg cttccccctc gacagggact
     1261 tcagtagccg gctggtggct tccccgatga acatgccgtc gccggtcgcc ggctcgagca
     1321 gccgcgcttc ggcgagattc gcatcggctt tccagccgat agaatccaga agtctgatga
     1381 ccacctcgcg tcgcgtataa acaccgcaac gtcggtgaat ctcttccata tcgggcgata
     1441 cctcgcaatt cgcggtggat ggaacagttc aagcattggc cgcgctactc atgtacttgg
     1501 ggtcgtgcca acatatgtgc atcgaatcag tagtttgagc ggatcaagtt gtcacccaat
     1561 taacaggcct caacgccatg gcggtcgcgg gtttgttcgc aggaatcgga ggcctcgaac
     1621 tcgcctttgc cgaggccggg tacgacgctg tcgtgctgtc cgagatcgac tcctcggcgc
     1681 agtcggtcct caggcatcgc tttccccgag cgacggtcgt cggcgacatc gctggaatac
     1741 ctgacttgcc agtgggcgtt gaaatcgtca cggctgggtt tccctgccaa aacctaagca
     1801 tggccggaca cggagcagtc ctccttccga gtctgtccgc gtgtgaaaaa tgcaggggtt
     1861 cacggcaggt tggaccgagg ccgtgccgga aggtactgaa cgactgaggt ggaaactcat
     1921 agccaacgcc gtttcggttc cagtagcggc ttgggtggcc tcccggatta ggactccggg
     1981 tccgattgtt gaccttgaat gcagatcgct gccggacgcg gccaagtggc ccgacgccgc
     2041 ttggaacgtt ggaaacggac gagttgcggt aagggtgggc gataggcccc tgaacttgtc
     2101 agcgcagtcc ataacagttt tcagggatac gacctggcct cgtctttcgg aacgagcgct
     2161 gcagggtttc cttcggagag cgacagaggg aaacctgcgc ttcccaccag gcttcatcga
     2221 caggctccga gcagcgcgag caacattttc ctcagccgct tgatttgcaa gtagaccttg
     2281 aagacgcccg aaattgagac cttcgcgctc tcctttcggg catttttgcg ccggcaaagc
     2341 cagttttatg ccagccttat atgatcgatt aggttggcgc ggtgccgttc gctcgctaag
     2401 acagagccat gattggaagc gaaccgcgat accaggcctt tctcgaaagc cgcgcgcgcg
     2461 aaggcctgcg gcggttcttg cttgccgtcg ccgcgggcga ggcgcggacc ctcgccgccg
     2521 agggcgggtc ctacgtcaac ttctccagca atgactatct tggcctgcgc ttcaacgcgg
     2581 ctttgatcga acgagcgatg acgtgggcca gggaatgggg cgtgggctcc ggcgcctcgc
     2641 gcctcgtcac cggtaatctc gacctgtttc ggccgatcga ggccaagctg gcgcgcctca
     2701 agggcaagca agccgcgctc gtcatggcgt cgggcttcca gaccaacgcc gccgcgctcc
     2761 aggcgcttct cgatcggagc gtgctcggcg ccgagccgct cgtcttcgcc gaccgcctca
     2821 atcacgcaag catgcatttc ggctgtgagg cggccggcgc ccggcagatc cgctaccgac
     2881 atggcgacgc cggtcatctc ggcgagcttc tcgatcgcca tcaagccgat gaccggccga
     2941 aattcatcct caccgagagc gtcttttcca tggatggcga cgtagcgccg ctcggcgaca
     3001 tcgctcgcct tgcgcgcgca catgcggctt cgttgatcgt cgacgatgcg catgcaaccg
     3061 gaattttggg ggaagcgggg tccggccttt cgggcgaggc cgacatcgtg atcggcacct
     3121 tctccaaggc gctcggcagc ttcggcggct ttgtcgcctc aagcgaaaaa gtgcgcgact
     3181 atctgatcaa ccgctgcgcc ggcgtgatct actcgaccgc gctgccgccg cccgtgctcg
     3241 gcgccatcga tgcagcactc gatatcgtcc ccggcatgga cgcggagcgc gcatatgtcg
     3301 cgaaacttgc cgaacggttt cgcaccggcg cgcgcgcaag cggactcgat atcggccgct
     3361 ccaccacgca gatcgtgccg atcctcgtag gccagcccga agccgcgctt accttgagcg
     3421 ggcggcttcg cgaggccggc ctttgggcca cctccatcag gccgccggcc gtgccgcccg
     3481 gcacggcgcg gcttaggctc gccttcaccg cggcgcatca agacgccgac gtcgatcgcc
     3541 tgctcgaggt cctttcggca aaggacgcgc cccgccgggc gcgggccgtg tgaaccgcag
     3601 cgatgcatgt cgtcctggtt catgggtggg gcttccatgc cggactctgg accgacttcg
     3661 ttcgccatct cgatggaaca ggcgtcacgc tggtcgatct cggtttcgtt tccggcggcc
     3721 ccaagggcga gagggactgg ccgccggatt cgatcgccgt gggccattcg ctcggcctct
     3781 tatggcttct cgcgcgcgga ggagggcagt tccgcgcgct cgtcagcgtg cagggtttcg
     3841 actgcttctg ctgccatgtg gcgttgccgc gggtcgccgc cctgaaacgt gggatcgagc
     3901 gc
//
LOCUS       DASUQP010000187        12897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1182202, whole genome shotgun sequence.
ACCESSION   DASUQP010000187 DASUQP010000000
VERSION     DASUQP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12897
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182202"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFY74_08370"
     CDS             <1..191
                     /locus_tag="VFY74_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931744.1"
                     /translation="VPSAPVPFGGALIYVPSEWVMPAEGGVERLMNVYVSMGVTPPLS
                     LPPKAKRSLPKPRKKPSS"
     gene            complement(267..1364)
                     /gene="ychF"
                     /locus_tag="VFY74_08375"
     CDS             complement(267..1364)
                     /gene="ychF"
                     /locus_tag="VFY74_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5931745.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTESAAAQAANYPFCTIEPNVGE
                     VPVPDPRLDRLAAIAKSSLVVPTRLTFVDIAGLVRGASQGEGLGNQFLAHIREVDAVA
                     YVLRCFEDTDVVHVEGRIDPIADADTIETELMLADLESLERRLPILEKKIRGQDKEAT
                     KTRELIERILPLLREGQPARLVAIAEGEQPAFKSLGLVTAKPVLYVANVDEASAASGN
                     RYSARVAERAAKEGAGCVAISAKIEAELAELSLEERDAYLAELGLPEPGLNRLIREGY
                     KLLGLITYFTAGPKEARAWTVPQGTRAQQAAGVIHTDFEKGFIRAETIAYDDYVALAG
                     EAGARDAGKLRLEGKDYVVKDGDVLHFRFAT"
     gene            complement(1378..2034)
                     /gene="pth"
                     /locus_tag="VFY74_08380"
     CDS             complement(1378..2034)
                     /gene="pth"
                     /locus_tag="VFY74_08380"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5931746.1"
                     /translation="MKLFVGLGNPKPDHAFNRHNAGFMAVDAIAAAYGFPVWRKRFDG
                     LVTESRLGQEQVLLLKPQTFMNASGRAAGEAVRFYKLNPADVVVFHDELDLASGKIRV
                     KTGGGVAGHNGLKSMTAHIGNDYVRVRIGIGRPGQSGRVKGYVLHNFAKADTAWLEPL
                     LGAIAAEAPLLAEGAIEKFQSRVAHAMQKVAIPEAPSPEAKPASAETLGARLRRWLGS
                     "
     gene            complement(2103..2822)
                     /locus_tag="VFY74_08385"
     CDS             complement(2103..2822)
                     /locus_tag="VFY74_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX5931747.1"
                     /translation="MAEAIELKAWARGRSGKGGARSERREGRIPGILYGDEQEPESIS
                     VDYRTIHQQIHTGHFQSTIYVLDVEGKKTRVIPRAVQVNPVRDFPIHVDFLRLGKDAH
                     VTVEVPVRFLNEAASPGLKRGGVLNIVRHEIAVRCPADAIPDHFEVDLTGLEIGDSVH
                     ISALKLPEGVRTTIAKRDFTVATIVGRAAEEPAPGAAVAAAAEPGAEAVEAAPDAEAE
                     DKDKDKEKEKPKEREKKKEKD"
     gene            3084..3299
                     /locus_tag="VFY74_08390"
     CDS             3084..3299
                     /locus_tag="VFY74_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX5931748.1"
                     /translation="MPQSVYKVIELIGTSTTSWEEAAKTAVERAAETLRDLRVAEVAA
                     QDLVITDGKVEAYRTKVKISFKYEGHH"
     gene            complement(3339..4271)
                     /locus_tag="VFY74_08395"
     CDS             complement(3339..4271)
                     /locus_tag="VFY74_08395"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5931749.1"
                     /translation="MKLVAGNSNRPLAEAIASYLKTSLAKCIVRRFADKEIFVEVQEN
                     VRGEDVFVIQSTSAPANDHLMELLILIDALRRASARRITAVIPYFGYARQDRKPGPRT
                     PISAKLVANMIVEAGADRVLTLDLHAGQIQGFFDIPVDNLFAAPVMVQDIKQVFPVDN
                     IMVVSPDVGGVVRARGLAKRIDAPLAIVDKRRERPGDSEVMNVIGEVSGRTCILVDDI
                     VDSGGTLVNAATALLDQGASLATGYITHGVLSGGAVARIVASQLKELVITDSIEPSEA
                     VKNAHNVRVLTIAPLLGEAIARTAEEQSVSSLFY"
     gene            complement(4396..5406)
                     /locus_tag="VFY74_08400"
     CDS             complement(4396..5406)
                     /locus_tag="VFY74_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931750.1"
                     /translation="MGADTRHVRGWVGRSVSVALAALALALLTGCGGGAGNPSSGGGF
                     LSGATGWFGGSTKIAVAPIIGTTPEISQQLTEALVFAGKERKLTLLPDGGKANYTLRG
                     YLVASSERQGAKISYIWDVTDAQGARVTRVSGDEIIAKRAGSDPWSAVDAAAIRSIAG
                     KTTSQLAASLPRGRLSSTPVAAAPPSSSTTSGSGMSASPAASPSAPAATARTARTASV
                     KSAGVLVQPVSGAPGDGQRSLTVALKKRLYAGGVKLANGTAVNVYMVKGSVKLTDASG
                     GKQSIRIDWQVLDPNGKKLGTVSQQNMITRGALNGPWGPIADAAAGAAAAGIIKLLPK
                     SS"
     gene            complement(5566..6345)
                     /gene="pgeF"
                     /locus_tag="VFY74_08405"
     CDS             complement(5566..6345)
                     /gene="pgeF"
                     /locus_tag="VFY74_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX5931751.1"
                     /translation="MVTPDKMMLSAPSLASIGGVRHGFFTRQGGVSNGIYASLNCGPG
                     SRDAAENVAQNRARVAELLGVDTNRLLTLFQKHSADAIVADKPWKAVPEADAIVTAKP
                     GLAIGILTADCAPVLFCDAAAGVIGAAHAGWRGALSGIVETTVKAMEKLGAKPERMVA
                     AIGPAISQKAYEVGAEFVEQFVATEPESASFFATDEGSGEPHFDLVGYVGERLQRAGV
                     GVVADLGLCTYCEETRLFSYRRSQHHGEDDYGRQISAIVLA"
     gene            complement(6324..7496)
                     /locus_tag="VFY74_08410"
     CDS             complement(6324..7496)
                     /locus_tag="VFY74_08410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5931752.1"
                     /translation="MSALPKPDAIGGESLAARLRDRIAREGPISVHDYMEACLADRRS
                     GYYTTRQPLGTAGDFITAPEMSQIFGELIGVWAAAVWQAMDEPSSVTVAELGPGRGTL
                     MADALRVWRSAPDFLARVSVALVERSPVLEAAQREKLSDSPAPLQWYAVLDDVPRGPL
                     IVIANEFIDALPARQIVRLGGVWRERTVTLADKSGFAFGAGAPVEHDALPSWLGEADV
                     ADGAIFEMRPATSRLIETIANRAQDSPIAALIVDYGHATSGIGDTLQAVHRHRFADPL
                     TAPGETDLTAHVDFAALKGAARARGLDAFGPMPQGQFLLKLGLQSRCNRLIRDATQDQ
                     REAILSGAARLADPRQMGALFKALALQSSGLAPPPPFADIEPPRLTASPSWLRPTR"
     gene            complement(7496..8323)
                     /gene="lgt"
                     /locus_tag="VFY74_08415"
     CDS             complement(7496..8323)
                     /gene="lgt"
                     /locus_tag="VFY74_08415"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5931753.1"
                     /translation="MQLFVIPYPVIDPVAIEIGPLAIRWYGLAYMAGIVLGWLYGRRL
                     VSRPELWGGKPPMTVAQADDFLLWITLGIVVGGRLGFVLFYEPSYFWQNPSEIPAVWH
                     GGMSFHGGLLGVVLAVYLFAKIKGINPLSLGDIASAATPFGLFFGRVANFINAEVVGR
                     VSDVPWAMVFPGAGDAPRHPSQLYEAFLEGIVLFIILRIATHRYDALTRPGTVFGLFL
                     VFYGLFRSLAEFVREPHAGHPLDIGILTPGIAYSIPMILIGLWLIWRARQKQALATP"
     gene            8489..8758
                     /locus_tag="VFY74_08420"
     CDS             8489..8758
                     /locus_tag="VFY74_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX5931754.1"
                     /translation="MTQTTGRFFDELGKLITDAAGAADGIRKEIEGIVRTQAERVLNG
                     LDVVQREDFEAVKAMAQKAREENERLSERIATLEAALAKSLSNKG"
     gene            8901..9317
                     /locus_tag="VFY74_08425"
     CDS             8901..9317
                     /locus_tag="VFY74_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5931755.1"
                     /translation="MRFMILVKATKESEAGVRPPDNLFAAMADYHEQLAKAGVLLDGS
                     GLQPSSKGWRIRYSGDKRTVVDGPFAETKELVAGYTMIQVKSREEAIEWTRRFPNPTI
                     DGSDCEIEVRQLFELEDFGESEVLDRFREIGLSTEK"
     gene            9340..9747
                     /locus_tag="VFY74_08430"
     CDS             9340..9747
                     /locus_tag="VFY74_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5931756.1"
                     /translation="MRFMSMVKSSEAQLTPPPKAFLEAMNRLIEESAKAGCVMVEAGG
                     LLPTSNGARLRLSGGKVTVMDGPFTEAKEVVGGYAMFDVASKDEMIKWTKRFLDLHRT
                     HLPGWEGEAEIRQVAGPGEKICDQARETQAAQV"
     gene            9790..11082
                     /locus_tag="VFY74_08435"
     CDS             9790..11082
                     /locus_tag="VFY74_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5931757.1"
                     /translation="MTATDTHRAIEAVFRIEQAKLIGGLTRMVRDIGIAEELAQDALV
                     AALEQWPRSGLPDNPGAWLMTAAKHKAIDRFRRDRLAERKAGELGYALEVEQETAEVD
                     LDAAPDDEVGDDLLRLIFIACHPILSPEAQAALTLRLLGGLTTEEIARAFLLPEPTIQ
                     QRIVRAKRTLSDKRVPFEVPRGAELEARLSSVLAVVYLIFNEGYAATAGDDWMRPKLC
                     EEALRLGRILAALAPEESEVHGLVALMEIQMSRFGARVGPSGEPVLLLDQDRARWDRL
                     LIRRGLAALARAEALNKASGRAPAPYLLQASMAACHARALSAEETDWARIAGLYAKLG
                     EVAPSPVVELNRAVALAMLFGPEAGLNLVDALADRSELKTYHLLPSVRGELMRQLGRF
                     DEARAEFERAAALTRNERERSLLLGRAAACQVNLDGPA"
     gene            11384..11881
                     /locus_tag="VFY74_08440"
     CDS             11384..11881
                     /locus_tag="VFY74_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX5931758.1"
                     /translation="MATIQPVFDHFTNPVDMVEQIATIHHWNFERNAPDELTLTVSGA
                     WCDYHISLNWRDDLEALHLACAFDFKVPKQRLAEIYRLMALVNEQLWLGHFDHWREDG
                     LLLYRHGLLLAGADTHVGQCEALLRAALEACERYYQAFQFVLWAGKSPEDALAATMLE
                     TQGHA"
     gene            11878..12699
                     /gene="proC"
                     /locus_tag="VFY74_08445"
     CDS             11878..12699
                     /gene="proC"
                     /locus_tag="VFY74_08445"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5931759.1"
                     /translation="MTLRLAGPLLLVGAGKMGGALLEGWLRQGFDPAAVFIQDPAPPP
                     EVRALAARHKIAAGDAPDLPAPASVIVIAVKPGLVEKLMPELEPLIGEGSVVLSIAAG
                     RTLAALARALPAGTAVVRAMPNVAASVGRSMTVACANSAVSREQALASNMLLEAVGDV
                     MWIEDESLFDAITAVSGSGPAYVFLLAECLEDAGRAAGLDAELARQLARATVEGAGEL
                     LQRSDLSPRELREKVTSPQGVTAAALEILMGEEAVKDLLTRAVAAAAKRSRELSS"
BASE COUNT         2226 a         4207 c         4290 g         2174 t
ORIGIN      
        1 tcgtgccatc ggcgccggtt ccgttcggcg gcgcgctgat ctatgtgccg tcggagtggg
       61 tcatgccggc cgagggcggc gtggagcggc tgatgaacgt ctacgtgtcg atgggcgtga
      121 cgccgccgct gtcgctgccg ccgaaggcga agcgctcgct tccaaagccg cggaaaaaac
      181 catcatcctg aggcgcgagc gtcgtttcca acctccccgc gtgggggagg ttggaaagtc
      241 cgctttcccg cgaatggtgc ggtagttcac gtggcgaagc ggaagtgcag cacgtcgccg
      301 tccttgacca cgtaatcctt gccttcaagc ctgagcttgc cggcgtcgcg cgcgcccgct
      361 tcgcccgcga gcgccacgta gtcgtcgtag gcgatcgtct cggcgcggat gaaacccttc
      421 tcgaaatcgg tgtggatgac gccggccgcc tgctgcgccc gcgtcccttg cggcacggtc
      481 caggcgcgcg cctccttggg ccccgcggtg aaataggtga tgaggccgag cagcttgtag
      541 ccctcgcgga tcagccggtt gagcccgggc tctggcagcc caagctcggc gagataggcg
      601 tcacgctcct cgagtgagag ttcggcgagc tccgcctcga tcttggcgga gatcgccacg
      661 cagcccgcgc cttcctttgc cgcgcgctcg gcgacgcggg ccgaatagcg gttgcccgac
      721 gccgccgagg cctcgtcgac attggcgaca tagagcacgg gtttcgcggt gacgagccca
      781 agcgatttga acgccggctg ttcgccctcg gcgatggcca cgagtcgcgc gggctgcccc
      841 tcgcgcaaga gcggcaagat gcgctcgatc agttcgcgcg tcttcgtcgc ttccttgtcc
      901 tgcccgcgaa tcttcttctc aaggatgggc aagcgccgct ccaagctttc gagatcggcg
      961 agcatcaact cggtttcgat cgtgtcggcg tcggcgatcg ggtcgatgcg gccctcgaca
     1021 tgcacgacgt cggtgtcctc gaagcagcgg agcacatagg cgaccgcgtc gacctcgcgg
     1081 atatgagcga ggaactggtt gcccaggccc tcgccctgcg aggctccgcg cacgagcccg
     1141 gcgatatcga cgaaggtgag ccgcgtcggc acgaccaggc tcgacttggc aatggcggca
     1201 agccggtcga ggcgcggatc ggggaccggg acctcgccga cattcggctc gatcgtgcag
     1261 aacggatagt ttgcggcctg ggccgccgca ctctcggtca gcgcgttgaa cagcgtggac
     1321 ttgccgacat tcggcaatcc gacgatgccg catttaaatc ccatgatcag ggcgccttca
     1381 cgagccgagc cagcggcgga gccgcgcgcc caatgtctcc gcagaggcgg gcttggcctc
     1441 aggtgagggc gcttccggga tcgcgacctt ctgcatggca tgcgcaaccc gcgactgaaa
     1501 cttttcgatc gccccttcgg cgagaagagg cgcctcggcg gcgattgcgc cgagcagagg
     1561 ctcgagccag gcggtgtcgg ccttggcgaa gttgtgcagc acatagccct tcacgcgacc
     1621 gctctgtccg ggacggccga tgccgatgcg cacgcgcaca taatcgttgc cgatatgagc
     1681 ggtcatggat ttgagcccgt tatgcccggc cacgcctccg cccgtcttca cccggatctt
     1741 gcccgaggcg agatcgagct cgtcatggaa gaccacgaca tcggcggggt tcagcttgta
     1801 gaaacgcacc gcttcgccgg cggcgcggcc cgatgcgttc atgaatgtct gaggcttcaa
     1861 cagcaacacc tgctcttgcc caagcctgct ttcggtcacg agcccatcga agcgcttgcg
     1921 ccagaccgga aagccatacg ccgcggcgat ggcatcgacc gccatgaagc cggcgttgtg
     1981 acggttgaaa gcgtggtccg gtttcggatt gccgaggccg acaaagagct tcatcaaagc
     2041 catcctgatt caacgcgcgg aggcgcgcgg gcttccgcgc accctcgggt cgaacgaagg
     2101 cgctaatcct tctccttctt cttctcgcgc tccttcggct tctccttttc cttgtccttg
     2161 tccttgtcct cggcctccgc gtcaggagcc gcctcgaccg cctcggcgcc gggctctgct
     2221 gccgcagcca cggccgcgcc gggtgcgggc tcttcagccg cccgtccgac gatcgtcgcc
     2281 accgtgaagt cgcgcttggc gatcgtcgtg cgcacgcctt cgggcagctt caacgccgaa
     2341 atatggaccg agtcgccaat ctcgagaccc gtcaggtcga cctcgaaatg gtccgggatg
     2401 gcgtcggccg ggcagcgcac ggcgatctcg tgacgaacga tattgagcac gccgccgcgc
     2461 ttgaggcccg gggatgcggc ctcgttgagg aaccgcaccg gcacttcgac ggtcacgtgc
     2521 gcatctttgc cgagccgcag gaaatcgaca tggatgggaa agtcgcgaac gggattgacc
     2581 tggaccgcgc gcgggatcac gcgcgtcttc ttgccctcga cgtcgagcac gtagatggtg
     2641 ctctggaaat ggccggtgtg aatctgctga tggattgtcc ggtaatcgac ggagatgctc
     2701 tcgggctcct gctcgtcgcc gtagaggatg ccgggaatgc gcccttcgcg ccgttcggag
     2761 cgcgccccgc ccttgcccga acggcctcgc gcccaagcct tgagttcgat cgcctcagcc
     2821 atcacgagcc tccaaacctc tcaaaatgat gaggggccgg aaagcggccc cgccatgtcg
     2881 aacgtgcggc gttccgcgcc gccatggaaa tcgaggaggc ttatagccct ttcgggaggg
     2941 ccctgacaat cggctcgtgg cggcatcgaa cggcccagac agacgggggc ggttcggcgt
     3001 aggatggccg tcttgtttct tcggagactg ctcgaaccac ccccgtcgca aaggcggacc
     3061 cgcgaggctc aaggaggaag tccatgccgc aaagtgtgta caaagtcatc gaactcatcg
     3121 gcaccagcac gacatcctgg gaggaagccg ccaagacggc cgtcgagcgc gcggctgaaa
     3181 ccttgcgcga cctgcgcgtg gcggaagtgg ccgcccaaga cctcgtcatc accgacggca
     3241 aggtcgaggc ctaccggacc aaggtcaaaa tatccttcaa gtatgagggt catcactagc
     3301 caggcatccg gcgggcacga gccgccggtc cgcgcaagct agtagaacag gctcgagacc
     3361 gattgctcct cggcggtccg cgcgatggcc tcgccgagga gcggagcgat ggtgagcacg
     3421 cggacattgt gggcgttttt caccgcctcg ctcggctcga tcgagtcggt tatgacaagc
     3481 tccttgagtt gcgaggccac gatgcgcgcg actgcgccgc ccgacagcac gccgtgggtg
     3541 atgtaaccgg tggcgagact cgcgccctga tcgagcaggg cggtcgcggc attgaccagc
     3601 gtgccgccgg aatcgacgat gtcgtcgacc aggatgcagg tgcggccgct cacttcgccg
     3661 atcacgttca tcacttccga atcgcccggc cgctcccgcc gcttgtcgac gatggcgagc
     3721 ggcgcatcga tgcgcttggc aagcccgcgc gctctcacca cgccgccgac gtcgggcgac
     3781 accaccatga tattgtcgac cgggaacacc tgcttgatgt cctggaccat gaccggcgcc
     3841 gcgaacagat tgtcgaccgg gatgtcgaag aagccctgga tctggccggc atgcagatcg
     3901 agcgtaagca cgcgatcggc gcccgcctcc acgatcatat tggcgaccag cttggccgag
     3961 attggcgtcc ggggtccggg cttccggtcc tgccgggcgt aaccgaaata ggggatcacc
     4021 gcggtaatgc gccgcgccga agcccgccgc aacgcatcga tgaggatgag gagctccatg
     4081 agatggtcgt tggcgggagc ggaggtcgat tggatgacga acacgtcctc gccgcggaca
     4141 ttctcctgca cctcgacgaa aatctcctta tcggcgaagc ggcgcacgat gcacttggcg
     4201 agggaggtct tgaggtagct tgcgatcgcc tcggcgagcg ggcggttgct gttgcctgca
     4261 accagtttca tcggtcgctc cttcgtcgcc gagggtcaag ccttgggcgg gctatgagcc
     4321 agcggcaagc ggcttttaac aagcgctcgc aggcgctgta aactcgcgcc gcgcgggaag
     4381 cctgtggatg acttgctagc tcgacttggg caggagcttg atgatgccgg cggcggcggc
     4441 tccggcggcg gcatcggcaa tggggcccca cggcccgttg agcgctccgc gggtgatcat
     4501 gttctgctgc gagaccgtgc cgagtttctt gccgttgggg tcgagcacct gccagtcgat
     4561 gcggatgctc tgcttgccgc cgctggcatc ggtcagcttg acgctgccct taaccatgta
     4621 gacgttcacg gccgtgccgt tggcgagctt cacgcccccg gcatagaggc gcttcttcag
     4681 cgcgaccgtc aacgatctct gcccgtctcc cggcgcgccg ctgacgggct gaaccaggac
     4741 gcctgccgat tttacgctcg cggtcctcgc cgtgcgcgcg gtcgcggctg gggctgaagg
     4801 actcgcggcg ggacttgcgc tcatgccgct gccgctcgtc gtgctgctgc tcggcggcgc
     4861 ggccgcgacc ggcgtggagg agaggcgccc cctgggcagg cttgccgcaa gctgcgaggt
     4921 ggtcttgccg gcgatgctcc ggatcgcggc ggcatcgacc gcgctccagg gatcgctgcc
     4981 ggcgcgtttg gcgatgatct cgtcgcccga gacgcgcgtc acgcgcgctc cttgcgcgtc
     5041 ggtgacgtcc caaatgtagg agattttcgc gccttgcctt tcgctcgagg cgacgaggta
     5101 gccgcggagc gtatagttgg ccttgccgcc gtcggggagc agcgtgagct tccgttcctt
     5161 gcccgcgaag accaaggcct cggtgagctg ctgagagatc tcgggcgtgg tgccgatgat
     5221 gggggccacg gcaatcttgg tcgagccgcc gaaccagccg gtcgcgccgc tcaagaaacc
     5281 gccgccgctg cttgggttcc ctgcgccgcc gccgcagcca gtgagcaagg caagcgcaag
     5341 tgcggcgagc gctacggata cggaacgccc gacccagccg cgcacgtgtc gtgtgtccgc
     5401 gcccaacaaa acactccctc tgttggccgc acagcaccgc tcgcgacaga actgtcgtga
     5461 cggaactccc aaccctctgc cgtcagctta cctgccccgg cccgctggcg tccagttaca
     5521 gcgtggaccg gattatggcc aaatccgcgt catgaagatg atgagtcagg cgagcacgat
     5581 ggcggaaatt tggcgtccgt aatcgtcttc accgtgatgt tgcgaccgcc gatagctgaa
     5641 gaggcgcgtt tcttcgcaat aggtgcaaag cccgagatcg gcgacgacgc ccaccccggc
     5701 acgctgaagt ctctcgccga catagcccac gaggtcgaaa tggggctcgc ccgagccctc
     5761 gtcggttgcg aaaaacgagg cgctttccgg ctcggttgcg acaaactgtt cgacaaactc
     5821 cgcccccacc tcgtaggcct tctgcgagat cgcgggcccg atggccgcga ccatgcgctc
     5881 gggcttggcc cccaacttct ccatggcctt gaccgtcgtc tcgacgatgc cggacagcgc
     5941 gccgcgccag cccgcatgcg ccgcgccgat cacccctgcc gccgcatcgc agaacaagac
     6001 cggggcgcaa tcggcggtca ggatgccgat ggccaggccc ggcttcgcgg tgacgatcgc
     6061 gtccgcttcg ggcacggcct tccagggctt gtcggccacg atggcatcgg cgctgtgctt
     6121 ctggaacagc gtgagcaggc ggttcgtgtc gacgccgagc aattcggcga cgcgcgcgcg
     6181 attttgcgcc acattttccg cggcgtcgcg cgagcccggt ccacaattga gggaggcata
     6241 aatgccgttc gagacgccgc cctggcgcgt gaagaatccg tggcgcacgc caccgatcga
     6301 ggcgaggctc ggcgccgaga gcatcatctt gtcgggcgta accatgatgg gctggcagtc
     6361 aatcgcgggg gctcaatgtc tgcgaaggga ggcggcggag caagcccgct cgactgaagc
     6421 gcgagcgcct tgaacaatgc ccccatctgg cgaggatcgg caagccgagc cgcgcccgac
     6481 aggatcgctt ccctctgatc ttgcgtggca tcgcggatga gacggttgca ccgcgattgg
     6541 agcccaagct tgagcaggaa ttggccctga ggcatggggc caaacgcgtc gagaccgcgc
     6601 gcgcgcgccg ctcccttgag cgcggcgaaa tcgacatggg cggtgaggtc ggtttcgccc
     6661 ggcgcggtta gcggatcggc gaaacggtgg cggtgaacgg cctgaagcgt gtcgccgatg
     6721 ccgctcgtcg catgaccgta atcgacgatc agcgcggcaa tgggcgagtc ttgcgcgcgg
     6781 ttggcgatcg tttcgatgag cctggaagtg gcgggccgca tttcgaagat cgcgccgtcg
     6841 gccacgtccg cctcgccgag ccatgagggc agcgcatcat gttcgacggg tgcaccggca
     6901 ccgaaggcaa acccgctctt gtcggcgagc gtgacggtgc gttcgcgcca aacccctccc
     6961 agtcgcacga tctgccgcgc ggggagggcg tcgatgaact cgttggcgat gacgatcaac
     7021 ggaccgcgcg ggacatcgtc gagcaccgcg taccattgaa gcggggcagg ggagtcgctg
     7081 agcttttcgc gttgcgccgc ctcgagtacc ggactcctct cgacgagagc caccgagacg
     7141 cgggcgagga agtccggcgc gcttcgccag acgcggaggg catcggccat gagtgtcccg
     7201 cgtccaggcc ctagctcggc gaccgtcacg gaggagggtt catccatcgc ctgccaaacg
     7261 gccgccgccc agaccccgat cagctcgccg aagatttggc tcatctctgg cgcagtgatg
     7321 aaatcccccg cggtcccgag cggttggcgt gtcgtgtagt agcctgaacg ccgatcggcg
     7381 agacaggctt ccatatagtc gtggacgctg atgggccctt cacgggcaat ccggtcgcgc
     7441 aagcgcgccg cgagcgattc cccgccgatc gcatcaggct tcggcagcgc gctcatcacg
     7501 gcgtggccaa agcttgcttc tgccgcgccc gccagatcag ccagagcccg atcaggatca
     7561 tggggatgga gtaggcgatg ccgggcgtga ggatgccgat atcgagcgga tggccggcat
     7621 ggggctcgcg cacgaactcg gcgagactgc ggaacaggcc atagaagacc aggaacaggc
     7681 cgaacaccgt gcccgggcgc gtcagcgcgt cgtagcgatg ggtggcgatc cgcaggatga
     7741 tgaacaggac gatgccttcg aggaacgcct cgtagagttg gctcggatgg cgcggagcgt
     7801 cgcctgcgcc cggaaagacc atagcccacg gcacgtcgct cacgcgtccc accacttcgg
     7861 cattgatgaa attggcgacg cggccgaaga acaggccgaa cggagtcgcg gccgaggcga
     7921 tatcgccgag cgagagcgga ttgataccct tgatcttggc gaagagatag acggcgagca
     7981 ccacgccgag gaggccgccg tgaaacgaca tgccgccatg ccacaccgcc gggatctccg
     8041 acgggttctg ccagaaatag ctcggctcat agaacagcac gaagccgagc cgtccgccta
     8101 cgacgatgcc gagcgtgatc cagagcagga aatcgtcggc ttgcgcgacc gtcatcggcg
     8161 gcttgccgcc ccacagctcg ggccgcgaca cgagacgccg cccataaagc cagccgagca
     8221 cgatgcccgc catgtaggcg aggccatacc agcggatggc aagcgggccg atttcgatcg
     8281 cgacgggatc gatcacgggg taggggatca cgaaaagctg catgaggctc tgccgggtcg
     8341 cggggaggca cgcgagcctt caccttgagc cgcacgcctg tcaaggcgcc gaaatcccgc
     8401 accgtccccg cttgcagtgg accgtcggcc gccccatagt tgcaaggagc caaacgcgct
     8461 ttggcggtga aacgtggatc caagaacgat gacgcagaca acaggccggt ttttcgacga
     8521 gctcggcaag ctcatcaccg acgcggccgg ggccgccgac ggcatccgca aggagatcga
     8581 aggcatcgtc cgcacccagg ccgagcgcgt gctcaacggc ctcgacgtgg tgcagcggga
     8641 ggatttcgag gcggtcaagg ccatggcgca aaaggcgcgc gaggagaacg agcgcttgag
     8701 cgaacgcatc gctacgctcg aagccgccct tgccaagagc ttgagcaaca agggctaact
     8761 ggccctcctc caaaccgccg gaaaaaaatg tttcgaccct gtcgatttcg accggcgcca
     8821 ttcgtcgtgt tggtagagcg cacatccggt gacaggatgg ctgggctcgg caaattcggc
     8881 aggacaagga gacaccgagc atgcgtttca tgattttggt caaagcgacc aaggagtccg
     8941 aagcgggcgt caggccgccg gacaacctgt tcgccgccat ggcggattat cacgagcaac
     9001 tggccaaggc cggcgtgctg ctcgacggca gcgggctcca acccagttcg aaaggctggc
     9061 gcatccgcta ttccggtgac aagcgcacgg tcgtcgacgg ccccttcgcc gagaccaagg
     9121 aactcgtcgc cggctacacg atgatccagg tcaagtcgcg cgaggaagcg atcgagtgga
     9181 cgcggcgctt ccccaatccc acgatcgacg gcagcgactg cgagatcgag gtccggcaac
     9241 tgttcgagtt ggaagatttc ggcgaaagcg aggttcttga ccgcttccgc gagatcggac
     9301 tctccaccga gaaataggca cgagaaaggg agcaaaacaa tgcgattcat gtcgatggtc
     9361 aaatcttcgg aagcacagct gacgcctcca cccaaggcct tcttggaggc catgaaccgg
     9421 ttaatcgagg aatccgccaa ggcgggttgc gtcatggtcg aggcaggcgg cttgttgcca
     9481 acgtcgaatg gcgcgcgcct caggctctcc ggcggcaagg tcactgtgat ggacggaccg
     9541 ttcaccgagg ccaaggaggt cgtcggcggt tacgcgatgt tcgacgtcgc gtcgaaggac
     9601 gagatgatca aatggaccaa gcgcttcttg gacctgcatc gaacgcatct gccgggctgg
     9661 gaaggcgaag ccgaaattcg ccaggtcgcg ggtccgggtg aaaagatctg cgaccaagcc
     9721 cgcgagacgc aagccgctca ggtctaagag gcttgcggca tcggcgagag ctgcgttagt
     9781 cggtagcccg tgacggctac cgacacgcac cgcgccatcg aggcggtctt caggatcgag
     9841 caggcgaagc tcatcggcgg cttgacgcgg atggtacgcg atatcggcat cgccgaggag
     9901 ctcgctcagg acgctctcgt cgcggcgctg gagcaatggc cgagatcggg cttgcccgac
     9961 aatcccggcg cctggctcat gaccgccgcc aagcacaagg cgatcgaccg tttccgccgc
    10021 gacaggctcg ccgagcgcaa ggccggcgaa ctcggctatg cgctcgaagt cgagcaggag
    10081 acggctgaag tcgatctcga tgcggccccc gacgacgagg tcggggacga tctgctgcgc
    10141 ctcatcttca tcgcgtgcca cccgatcctg tcgcccgagg cgcaggcggc actgacgctg
    10201 cgcctgctcg gcggcctgac caccgaagag atcgcccgcg ccttcctgct gccggagccg
    10261 accatccagc agcgcatcgt tcgcgccaag cggacgctga gcgacaagcg cgtgcctttc
    10321 gaagttccgc gcggggcgga gctcgaagcg cgtctctcct cggtgcttgc cgtcgtctac
    10381 ctcatcttca atgagggcta tgcggcgacg gccggcgacg actggatgcg gcctaagctc
    10441 tgcgaggagg cgttgcgcct cggccgcatc ctcgccgctc tcgcgcccga agagtccgaa
    10501 gtgcacgggc tcgtcgccct gatggagatc cagatgtcgc gcttcggggc gcgcgttgga
    10561 ccgtcgggcg agcccgtgct gctgctcgac caggaccggg cgcgctggga ccggctcctc
    10621 atccgccgcg gccttgccgc gcttgctcgg gcggaggcgt tgaacaaagc ctcagggcgc
    10681 gcccccgcgc cgtacctcct acaagcgtcg atggccgcct gccatgcgcg cgcgctgagc
    10741 gccgaggaga cggactgggc aaggatcgcg gggctctacg ccaaactcgg ggaggtcgcg
    10801 ccgtcgccgg tggtcgagct caaccgggcg gtggcgctgg cgatgctgtt cggtcctgag
    10861 gccgggctca atctggtcga tgcgcttgcc gatcggtctg agctgaagac ctaccacctg
    10921 ctgccgagcg tgcggggcga gctgatgcgg cagctcgggc gtttcgacga agcgcgcgcg
    10981 gagttcgagc gggcggcggc gctgacgcgc aacgagcgcg aacgcagtct tctcctcggc
    11041 cgcgccgccg cgtgccaggt gaatctcgac ggtcctgcgt gacccacgtg gaaataacct
    11101 ccccctgcct tcgccgaagc caagctccgg cttcgcgcag gcaggtcgcg gggaggtcgg
    11161 cgcgctgcgc tcgccgccct cccgtcatgc ggaggttgat ttcgtgcaaa cggagagggg
    11221 atttgcagaa agcaaccatc aatccacaac tatcttcgcc caatccctct tgcgcggcgt
    11281 gcccgccttg gtgcttccgt ttcgcccatt atataactga ttcacaaagt gattcgcgga
    11341 cgggcaagac gcgaaacgct gccggggcca acagaggaga cgcatggcga cgatccaacc
    11401 ggtcttcgac catttcacca atccggtgga tatggtcgag caaattgcca ccatccacca
    11461 ctggaacttc gagaggaacg cgcctgacga gctgacactc accgtgtctg gcgcgtggtg
    11521 cgactatcac atctcgctca actggcggga cgatctagag gcgctgcatc tcgcatgcgc
    11581 cttcgacttc aaggtgccca agcagcgcct tgccgagatc taccggctga tggccctggt
    11641 caacgagcag ctttggctcg gtcatttcga ccactggcgc gaagacggac tgcttctcta
    11701 ccgccatggc ctgctgctcg cgggcgcgga cacccatgtc ggccaatgcg aggcgcttct
    11761 ccgggctgcc ctcgaggcct gcgagcgcta ttatcaggcg ttccaattcg tgttgtgggc
    11821 gggcaagtcg cctgaggatg cgcttgccgc cacgatgctc gagacgcaag gacacgcatg
    11881 accctccgcc tcgccggccc gctgctcctg gtgggcgcgg gcaagatggg cggggcgttg
    11941 ctcgaaggtt ggctccggca aggcttcgac cccgccgccg tcttcatcca ggatccggcg
    12001 ccgccgccgg aggtccgggc gcttgccgcc cgccacaaga ttgccgcggg cgacgcaccc
    12061 gatctgccgg caccggcttc cgttatcgta atcgcggtca agccgggtct cgtcgagaag
    12121 ctcatgccgg agcttgagcc tctgatcgga gaggggagcg tcgttctctc catcgccgcg
    12181 ggccggacgc ttgccgccct cgcgcgcgcg cttccggccg gaacggcggt cgtgcgcgcc
    12241 atgcctaacg tggccgcgtc cgtcggccgg agcatgaccg tggcgtgcgc caattccgcc
    12301 gtatcgcgcg agcaggcgct cgcctcgaac atgctgctcg aagcggtcgg cgacgtcatg
    12361 tggatcgagg acgaaagcct gttcgatgcc atcacggcgg tgtcggggag cggcccggcc
    12421 tacgtcttct tgcttgcgga gtgtcttgaa gacgcgggcc gtgccgccgg tctcgatgcc
    12481 gagcttgcgc gccaattggc gcgggcgacg gtcgagggcg ccggcgaatt gctgcagcgc
    12541 tctgaccttt cgccgcgcga gctcagggag aaggtgacct cgccgcaagg ggtcacagcc
    12601 gcggcgctcg aaatcctcat gggcgaggag gcggtcaagg atcttctcac ccgcgccgtc
    12661 gccgccgccg ccaaacgctc ccgcgaactg tcgtcttagg gcaaccctcc gcgcctcttt
    12721 tctaacctcc ccccctgcct tcgccgaagc gaagctccgg cttcgcgcag gcaggtcctg
    12781 gggaggtccg acgacgcgct ttattctgcg ctgacgcgtt gctgtttgag cgcaaagcgc
    12841 gccgggtggg gggcctggcc ttcaagcgtc aaccacgttt tcctactgta agccgga
//
LOCUS       DASUQP010000188         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1862952, whole genome shotgun sequence.
ACCESSION   DASUQP010000188 DASUQP010000000
VERSION     DASUQP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862952"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFY74_08450"
     CDS             complement(<1..184)
                     /locus_tag="VFY74_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931760.1"
                     /translation="MLRFINICLVLGLVALAYVIYQVKYEARALDAEIAAVNKRIDEE
                     RDAIAVFRAEWSLLNRP"
     gene            complement(212..1240)
                     /gene="rsmH"
                     /locus_tag="VFY74_08455"
     CDS             complement(212..1240)
                     /gene="rsmH"
                     /locus_tag="VFY74_08455"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5931761.1"
                     /translation="MMAGRGSPAAAGGPARHIPVMLSEVLAALDPKDGEVVVDGTFGA
                     GGYSQAILERADCKLIAIDRDPQAFRVSGELAAKYPGRLLAVLGRYSEMEAIAADQGF
                     TAVDGVALDLGVSSMQLDAPERGFSFMQDGPLDMRMGEDGLSAGDIVNTLSEQELAEI
                     VGKLGEERRARAIAKAIVARRQDKPIATTRELADLVAKVLGRKRDETKHPATRTFQAL
                     RLFINEELDELAKGLAAAERLLRAKGRLAIVTFHSLEDRIAKRFLASRSAPPPRASRH
                     LPEAAGEILAPSFQLLNRRPLEPSKGEIARNPRARSARLRAAMRTAAPAHPLDLAALG
                     VPRLGVAV"
     gene            complement(1237..1719)
                     /gene="mraZ"
                     /locus_tag="VFY74_08460"
     CDS             complement(1237..1719)
                     /gene="mraZ"
                     /locus_tag="VFY74_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5931762.1"
                     /translation="MDQFVSTFTNRIDTKGRVSVPASFRTVLAKDGLDGIYCYPSLDA
                     PALDAGGKRLVEKINALVEDLAPYSDEKDQLATALFGTSEILSIDQDGRTILTERLRE
                     YAGITSHITFVGLGEKFQMWEPKRFESHLAGARQKVRDLRKLLGAGRRGSGGPEGARE
                     "
     gene            2145..3071
                     /locus_tag="VFY74_08465"
     CDS             2145..3071
                     /locus_tag="VFY74_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5931763.1"
                     /translation="MAKRELKPTFAAAAIALIFGSCALSAGDGAGGPFARLGPPEAPV
                     NLYYQEQGKGPPVLLLHGFGASTFTWRHVIPELAQTHRVIAVDLKGFGQSDKPFDERY
                     SVLDQAELLAQLIEDKDLRDLTIVGHSFGGGVALRLALDANDRLGGRIVRLVLLDSIA
                     YPQQIPVFFRLLDMPLVSQVGVRMVPPSVQTRVALQIAYHDDSKIDPEQVEIYAAPLK
                     TAAGKHAIVQSARQIVPEDIDTLPERYKTITLPTLILWCDHDRIVPLDVGLKLRRTLP
                     NSTLRLVEGCGHMPQEEQPETTLTLLKGFIGG"
     gene            complement(3129..3546)
                     /gene="rnpB"
                     /locus_tag="VFY74_08470"
     ncRNA           complement(3129..3546)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFY74_08470"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3673..3948
                     /locus_tag="VFY74_08475"
     CDS             3673..3948
                     /locus_tag="VFY74_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX5931764.1"
                     /translation="MAERTTVRLPEDLVRRAKRKAAAEGRTLTALIEDGLRLVLADGQ
                     KKAKVKRILPPVSDAKGGPLPGIDITDGSALQEMDDLEYVERMKHFK"
     gene            3945..4373
                     /locus_tag="VFY74_08480"
     CDS             3945..4373
                     /locus_tag="VFY74_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX5931765.1"
                     /translation="MILPDVNVLIYAFREGVPEHAICRPWLDRVIESDARFGISPLSL
                     AAVVRVTTNPRLYPAPTSLDAAFRFCDNLLSQDHCQIVEPGDRHFDIFKRLCTETNTR
                     GPRVTDAWFAALAIEWGCEWVTLDRDFARFPGLKWQVPMA"
BASE COUNT          788 a         1417 c         1431 g          808 t
ORIGIN      
        1 ctgggcggtt gagcaggctc cactcggcgc ggaagacggc gatcgcgtcg cgctcctcgt
       61 cgatgcgctt gttgacggcg gcaatttccg cgtcgagcgc ccgggcctcg tatttgacct
      121 gatagatgac ataggcgagc gcgacgagac cgagcacgag acagatattg atgaagcgca
      181 gcatggcgtt actcacgaac gaaagcgacc ttcacacggc gaccccaagc cgcggcaccc
      241 cgagcgcggc aaggtcgaga ggatgagcgg gcgccgcggt gcgcatggca gctctaagcc
      301 gcgccgatcg ggcgcgcggg ttccgcgcaa tctccccctt gctcggttct aacggacgac
      361 ggttaagaag ctggaagctt ggggcaagaa tttcgcccgc agcctcggga aggtggcgcg
      421 aggcgcgcgg gggcggcgcg ctccggctgg ccagaaagcg cttggcgatg cggtcctcga
      481 gcgaatgaaa ggtgacgatg gcgagccgcc ccttcgcccg gagcagccgc tcggcggcgg
      541 caaggccctt ggccaattcg tcgagctcct cgttgatgaa gagccggagc gcctggaagg
      601 tacgggtggc gggatgcttg gtttcgtcgc gtttgcggcc gagaaccttg gccacgagat
      661 cggcaagctc gcgcgtcgtc gcgatcggct tatcctggcg ccgcgcgacg atcgccttgg
      721 cgatggcgcg ggcacggcgc tcctcgccaa gcttgccgac gatctcggca agctcctgtt
      781 ccgagagcgt gttgacgata tcgccggcgg ataggccgtc ctcgcccatg cgcatatcga
      841 gcggcccgtc ctgcatgaag gaaaagccgc gctccggcgc gtcgagctgc atggaggaga
      901 cgccgagatc gagcgccacg ccatcgacgg cggtgaagcc ttggtcggcg gcgatcgcct
      961 ccatctcgct atagcggccg agaacggcca gcaatcggcc cggatatttc gcggcaagct
     1021 ctccgctcac gcgaaaggct tgcggatcgc ggtcgatggc gatgagcttg cagtcggcgc
     1081 gctcaagaat ggcttggctg tagccgccgg cgccgaaggt gccgtcgacg acgacctcgc
     1141 cgtccttcgg atcaagcgcg gcaagaacct ccgagagcat cacgggaatg tggcgggccg
     1201 gtccgccggc ggcagccggg ctgccgcgac ccgccatcat tcccgtgctc cctctggccc
     1261 gccgcttccg cggcgccccg cgccgagtag cttgcgaagg tcgcgcactt tctggcgcgc
     1321 ccccgcgaga tgcgattcaa atcgtttcgg ctcccacatc tggaacttct cgccgagacc
     1381 gacgaaggta atgtgtgagg tgatgcccgc gtattcacgc aggcgttccg tgagaatggt
     1441 gcgtccgtcc tgatcgatgc tgagaatttc gctcgtgccg aacagagcgg tcgcgagctg
     1501 atccttctca tcggaataag gagcgaggtc ctcgaccaga gcgttgatct tttcgaccag
     1561 gcgcttgccg ccggcgtcga gcgcgggcgc atcgagggag gggtagcagt agatgccgtc
     1621 gaggccgtcc ttggcgagaa cggtgcggaa ggaagcgggg accgaaacgc ggcctttggt
     1681 gtcgatccgg ttggtaaatg tcgacacaaa ctggtccatc tccccctcac gcacccgagc
     1741 tgacgcgggg accgttgcaa agcctggggt gcaggcccat tgcccgaaaa aaatttgggt
     1801 gggagaggcg gcgcagatcc ccccgtggcc tctaaggaac gctgccggcc tcacccaccc
     1861 tactcaccga acgggcaaaa tcgggatatc atgggattat atgggcgtca atggaggagc
     1921 atcggggaag agcgggtttt cccgggaaaa tggcgcaaat gagccatacc gatcttgttt
     1981 tttggttaac cggaggcgat tgccgcttcc cggcgaagcg cggtagaacg ggaaaaacta
     2041 ggtatcccga cacgttcgcg cgctgattcg cgggcgcttt cgaccgagac agggcgctgc
     2101 cttacgtttt cgacgcgcga tcgcgaacgg gtcttgaggg gtgcatggcc aaaagagagt
     2161 tgaagccgac gttcgcagct gcggccatcg ccctcatctt cggctcgtgc gcactgagcg
     2221 ccggtgacgg cgcgggtggt ccgtttgctc ggcttggtcc gcccgaggct ccggtcaacc
     2281 tctactatca ggaacagggc aagggacctc cggtcctcct cctgcatggg tttggcgcaa
     2341 gcactttcac gtggcggcac gtcatccccg agcttgccca gacgcacagg gtcatcgccg
     2401 tcgacctcaa gggtttcggc cagtccgaca agccgttcga cgagcgctat tcggtgctcg
     2461 atcaagccga actgctcgcc cagctcatcg aggacaagga cctgcgcgat cttacgatcg
     2521 tcggccattc ctttggcgga ggggtcgctc tgcgtttggc gctcgacgcc aatgatcggc
     2581 tcggtggccg catcgtccgg ctcgtgcttc tcgactcgat cgcctatccg cagcagatcc
     2641 cggtgttctt ccgcctgctc gacatgcctc tcgtttccca ggtcggcgtg cgcatggtgc
     2701 cgccttcggt gcagacgcgg gttgcgttgc agattgccta tcacgacgac agcaagatcg
     2761 atcccgagca ggtcgaaatc tatgccgcgc cgctcaagac cgcggccggc aaacacgcca
     2821 tcgtccagag cgcgcggcag atcgtgcccg aggacatcga tacgctcccg gaacgctaca
     2881 agacgatcac gctgccgaca ctgatcctgt ggtgcgacca cgaccgcatc gtgccgctgg
     2941 acgtgggtct gaagttgagg cgcaccttgc cgaactccac tttgcggctg gtcgagggtt
     3001 gcggacacat gccgcaagag gagcaaccgg aaacgacgct gacgctgctc aagggcttca
     3061 tcggcggctg atcgagcggc acaaaattgg aggccggcgc gcagggttcg cccacgcgcc
     3121 ggccgtatca tgtcaccagg cctgtaagcc gggttctgta ccacgcgcgc cgaagcgaac
     3181 gcggcgatgg ccattcatct tgggcacgcc ttgcggcggg cctcatgcaa ccaacccggg
     3241 cggctgatcc ggaagccgat tggaagcggt ctcccgctcc cgcgccgccc ctattcggtc
     3301 ttgctcccgg tggggtttgc cgtgccgccc ttgttgccaa gaacgcggtg cgctcttacc
     3361 gcaccctttc acccttacct gccactcgcc cgaaggcgat ggtctcggcg gtatgctttc
     3421 tgtggcactg tccctggggg ttaccccgcc ggaggttatc cggcaccgtg cttccatgga
     3481 gcccggactt tcctccccgc ccaattacgc aaaacattga ggcggagcgg ccatccggcc
     3541 cgctgacaag cccaataatg gcgtcgatgg cgtccaaatc aagaccactc cacccgtatg
     3601 gctaagagtc tgcggcagct cgagcgccgg gcgcccttga catatcggcg ctcgcgccat
     3661 atgtcttgac atatggcaga gcgaaccacc gtcaggctcc ccgaagacct cgtccgacgc
     3721 gccaagcgaa aagcggcggc ggagggccgg accttgaccg cgctgatcga ggacggtttg
     3781 cgcctcgtgc ttgctgatgg tcagaagaag gcgaaagtga agcgcatcct gcctcccgtg
     3841 agtgacgcca agggcgggcc cttgcccgga atcgatatca ccgatggatc cgcgctccag
     3901 gagatggatg acctggaata tgtcgagcgc atgaaacact tcaaatgatc ctcccggacg
     3961 tgaacgtcct catctatgcc ttccgggaag gcgtgcctga acatgcgatc tgccgcccgt
     4021 ggcttgaccg ggtgattgag agtgacgcgc gatttggcat atcgccgttg tcgcttgcag
     4081 ccgtcgtacg cgtcacgacc aacccgcgcc tctacccggc gcccacttca ctcgatgcgg
     4141 ccttcaggtt ttgcgacaac ctcctgagcc aagatcactg tcagatcgta gagcccggcg
     4201 accggcactt cgacatcttc aagcgcctct gtaccgagac aaacacccgc ggcccgcgtg
     4261 tcaccgatgc ctggttcgcg gccctcgcaa tcgaatgggg ctgcgagtgg gtcacgctcg
     4321 accgcgactt tgcccgcttt ccaggcctga agtggcaggt gccgatggcc tgatcggggc
     4381 gtgtgaccgg gaggcgcggc ccgctcgtaa cggcattttg atgacttttt agccttcggt
     4441 tccc
//
LOCUS       DASUQP010000189         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1182641, whole genome shotgun sequence.
ACCESSION   DASUQP010000189 DASUQP010000000
VERSION     DASUQP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182641"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..296
                     /locus_tag="VFY74_08485"
     CDS             42..296
                     /locus_tag="VFY74_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931766.1"
                     /translation="MVNIYLFIELLHTANAVATVDTLKALEMQNCKFTNVVVLSDEKV
                     VGQLDCKDSIDGDKAILNKIAKVDGVVQTNIIAVVRPTKG"
     gene            complement(558..791)
                     /locus_tag="VFY74_08490"
     CDS             complement(558..791)
                     /locus_tag="VFY74_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931767.1"
                     /translation="MAKCEQCGNDYDKSFAVTMGGRTMIFDSFECAIAALASNCPHCG
                     TRIIGHGVQHGDTIFCCVHCAKHEGVTGLRDRD"
     gene            complement(1007..>2077)
                     /locus_tag="VFY74_08495"
     CDS             complement(1007..>2077)
                     /locus_tag="VFY74_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX5931768.1"
                     /translation="FNIGSPKQLGEILFDKMSLAGGRRTKTGAWSTDAAALEELAAEG
                     HDLPRKVLDWRVLAKLKSTYTDALPAYIHPETGRVHTSYALAATTTGRLSSQEPNLQN
                     IPVRTAEGRAIRKAFVAEKGRKLISADYSQIELRVLAHMADTPTLRQAFADGLDIHAM
                     TASEMFGVPIEGMEPAIRRKAKAINFGIIYGISSVGLAAQLGISRSEAGAYIKTYFER
                     FPGIRDYMEAMKAEARRQGYVKTLFGRKVHYPEINTKNPSLRGNYERAAINAPIQGTA
                     ADIIRRAMIRMGPALAEAGLSARMLMQVHDELVFEAEESEVEKTMEVAKSVMEKAPEP
                     ALKLTVPLKVDARSGDNWEAAH"
BASE COUNT          362 a          676 c          648 g          391 t
ORIGIN      
        1 aatcatagcg gcgacttcag ccaggccgag gaacgatccc aatggtcaat atctatctct
       61 tcatcgagct cttgcatacg gccaatgccg tcgcgaccgt cgacacgctg aaggcgctcg
      121 agatgcagaa ctgcaagttc accaatgtcg tggtcctgtc cgacgagaag gtcgtggggc
      181 agctcgactg caaggattcc atagacggcg acaaggcgat cctcaacaag atcgccaagg
      241 tcgacggcgt ggtgcagacc aacatcatcg ccgtggtccg cccgacgaaa ggctgattcg
      301 cgcgtcatgc cgaatcgtca tcctgaggtg ggcgtgcaac aaattcccgc tctcccctta
      361 ggggagagca cgtgcgccgt ttggcgcgcg gtgaggggta atcgggcgaa ggcccacgtc
      421 agaaagattc acccctcacc cttcccgcca ttcgtcgcgc ttcgcgcgtc gggcgggaag
      481 ccctctccct caaggggaga gggaaatcga ctccgtcata ccggcgaaag ccggtatcca
      541 gcaccacagg acccaagcta atcgcggtcg cgcaagccgg tgacgccttc gtgcttggcg
      601 caatggacgc agcagaagat cgtatcgccg tgctgcacgc catgcccgat gatgcgcgtg
      661 ccgcaatggg ggcagttgga cgcgagcgcc gcgatcgcgc attcgaaact gtcgaagatc
      721 atggtgcggc cgcccatggt gaccgcgaaa ctcttgtcgt aatcgttgcc gcactgctcg
      781 catttcgcca tggctcgcct ccttttagaa tcctgtagcc aagggtctca caatgtgtac
      841 gttccacaac ctcttcgtca tgcccggcca tgacggcgac gagcgggaga cgggtcgccc
      901 gaactcaaca ccccctcatg cggaggaggg ccgaaggccc gtctcgaagc acgcaccctt
      961 cgagccggcc gcgtccgcga cctcctcagg gtgaggatta caggccttaa tgcgcggctt
     1021 cccagttgtc gcctgagcgg gcgtcgactt tcagcggcac tgtcagcttg agcgcgggct
     1081 ccggcgcctt ctccatcacg ctcttagcca cctccatagt cttctcgacc tcgctctctt
     1141 ccgcctcgaa cacgagctcg tcatgcacct gcatcagcat gcgcgccgat aagcccgcct
     1201 cggcgagcgc aggccccatg cggatcatcg cgcggcggat gatgtccgcg gcggtgccct
     1261 ggatcggcgc gttgatcgcc gcgcgctcgt aattgccgcg caagctcggg ttcttggtgt
     1321 tgatctcggg gtagtgcacc ttccgcccga acagggtctt cacgtagccc tggcggcggg
     1381 cttccgcctt catcgcctcc atatagtcgc ggatgccggg gaagcgctcg aaataggtct
     1441 tgatataggc gcccgcttcg ctgcgcgaga tgccgagctg ggcggcaagc ccgaccgagg
     1501 agatgccata gatgatgccg aaattgatgg ccttggcctt gcgcctgatg gcgggctcca
     1561 tgccctcgat cggcaccccg aacatctccg acgccgtcat ggcgtgaatg tcgagcccgt
     1621 cggcgaaggc ctggcgcagc gtcggcgtgt cggccatatg cgcgagcacg cgaagctcga
     1681 tctggctgta atcggccgag atgagtttcc tgcctttctc ggccacgaac gctttgcgga
     1741 tggcgcggcc ctccgccgtg cgcacgggga tgttctgaag gttcggctcc tgcgacgaga
     1801 gccgccccgt ggtggtcgcc gcgagcgcgt aggaggtatg cacgcgcccc gtctcgggat
     1861 ggatataggc ggggagcgcg tcggtatagg tgctcttgag cttggcgagc acgcgccagt
     1921 cgagcacttt ccgcggcaga tcgtggcctt ccgccgcaag ctcttcgagc gccgcggcgt
     1981 ccgtgctcca ggcgcccgtc ttggtgcgcc gcccgcccgc aaggctcatc ttgtcgaaca
     2041 ggatctcgcc gagctgcttc ggcgagccga tattgaa
//
LOCUS       DASUQP010000190         6894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1048403, whole genome shotgun sequence.
ACCESSION   DASUQP010000190 DASUQP010000000
VERSION     DASUQP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048403"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFY74_08500"
     CDS             <1..644
                     /locus_tag="VFY74_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX5931769.1"
                     /translation="AEQILQALAQEPGTGTNFTMVRFGTVLDSSGSVVKLFRSQIRTG
                     GPVTVTHPEVIRYFMSIPEAAQLVIQAGTMATGGEVFVLEMGTPVKIDDLARTMIRLS
                     GLEVRDEKHPDGDIAIAYIGLRPGEKLYEELLIGENTTGTSHPRIFKNSEPFVPFEEL
                     AAVLERLDDAIQRLDEAEMQELLRSVVEGYVPASSEPQIAGKGAWEPASRTLH"
     gene            650..1663
                     /locus_tag="VFY74_08505"
     CDS             650..1663
                     /locus_tag="VFY74_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX5931770.1"
                     /translation="MTEPQPVLWGAILVAGCAGLSAFLIFLLRPFFVRHLMAHPNARS
                     SHKIPTPQGAGLAVMLSVLAGSALAILISGPEAEPSLIPVLGIAAGLTILGGIDDALT
                     LLVSWRLLGHALAAVALVLSLPEGFQLFPGLMPLVVERALIAFGTVWFINAVNFLDGL
                     DWMTVVQVVPIALGIAILEALGAVPATIGVLALALLGATLGFAIFNKHPARIFLGDAG
                     SLPIGLCLAFMLIFVAKAHLAAALLLPLYTMADATLTLARRMLAGERISSAHRTHFYQ
                     RGVAAGLSVPQVTARVLLLQVLLVGLAILTVLARSLTVDILCLGLGLLATGFALFALA
                     KGR"
     gene            1665..2600
                     /locus_tag="VFY74_08510"
     CDS             1665..2600
                     /locus_tag="VFY74_08510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5931771.1"
                     /translation="MKGRVLVTGASGFIGKRLTTALARAGWEVRAAARDPAGVPTASG
                     VERIAMDDLARGCDWPALLEGVTHVAHLAGIAHAPGTLPEALYARINAESVGEFADAA
                     RGKIARFVFLSSVRAQAGLSADRPITEKDIPAPTDAYGRAKLEGERLLAESGVPFTVL
                     RPAVVYGKGVKGNIAALATLARTPMPLPFAGLSNRRSLLALDNLVSAISLVLDAREAA
                     NETFVVADAEPISVANLVAAMREGLGRSPHLVKVPLGGVKRLMRSFGKEAEWERISGN
                     FVVDASKLRGIGWRPAIATRDGIAKMMRSENGTGG"
     gene            complement(2773..3897)
                     /locus_tag="VFY74_08515"
     CDS             complement(2773..3897)
                     /locus_tag="VFY74_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5931772.1"
                     /translation="MARRILYVVTEDWYFLSHRLPMARAAKAAGYEVHVATRLNDGKA
                     AIEREGFVPHALAWSRGSLSARGSLSAIWALRRLLRDLRPAILHNVSLKPVLLGTVAS
                     LGLSATAVVNSVTGLGTLFIGEANVKESARQAVRLALSRLLRRKRSRTIVQNPDDRAF
                     VVALGAPPETMVLIPGSGVDTNHLTPLPEPPAPVTAAYVGRMLADKGVLTLIEAFSLL
                     AERGTALKLLLAGDCDRENPGSLAPEQLREFASLYGIEWLGHVADVRKVWARAHFAVL
                     ASRREGLPKSLLEAAACGRAMVATDAPGCREIAIAGETAVTVPVGDAKALAHAMARLA
                     GDPELRKRFGANARRLAESKFSAEAIGRETVALYDSLIPS"
     gene            3884..4339
                     /locus_tag="VFY74_08520"
     CDS             3884..4339
                     /locus_tag="VFY74_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931773.1"
                     /translation="MRRAIGQGFYQRIRGCGTEGAAAIFPQSCRLCALPLAAITRYLL
                     LRLTEERLSGDVMKYLGLVLICCAFAVQPAQAFEIQGEKATLEDGGAQLTAPANQFLT
                     PDLSKGSSLALPYIGETDSGLISDYGNAISIPAPGVDKPAPAWALTPSR"
     gene            4467..4694
                     /locus_tag="VFY74_08525"
     CDS             4467..4694
                     /locus_tag="VFY74_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931774.1"
                     /translation="MHKVRIAVALVAALLVAGLVSWTAEATTAAGTAGLPETIKKYSL
                     IEKAACGGYGKHCPPGYTWVCRPNRCFCARC"
     gene            4973..6097
                     /locus_tag="VFY74_08530"
     CDS             4973..6097
                     /locus_tag="VFY74_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5931775.1"
                     /translation="MPRLTDKLLRGLKPPKSGQIDVWDELLPSFGVRIGVSGHKSFFV
                     GTRVNGRYRRITLRPAYDALSLADARSQARKIIADAHAGVGPEVRRQREAKSTFGAVA
                     TAFMQDFAHSHRTRGEMQRKIDVDLVDWRDRPIADIKRGDIKELIRLKARTAPIAANR
                     LLSLITKIFNWALKEELIEASPAMQIDRPGKETDRERFLSADEIRIAWAAFDKLGYPW
                     GSLYKMLLVTGQRRGEVAGMRWGEISPDGWTLPGGRAKKGQGHLVPLSTLAREILDDL
                     PELGKLVFRSRTDIPLAGWSKTAKRLQSLQMQPWHLHDLRRTFATHLRTLGVDRLVVS
                     KLLNHAEGGVTKTYDRWAADLEKADAMERWAIRLREIVGL"
BASE COUNT         1237 a         2164 c         2193 g         1300 t
ORIGIN      
        1 tggccgagca gatccttcag gcgctcgcgc aggagcccgg caccgggacg aatttcacca
       61 tggtgcggtt cggcaccgtg ctcgattctt ccggctccgt ggtcaagctg ttccgcagcc
      121 agatcaggac cgggggcccg gtgaccgtga cccatcccga ggtgatccgc tacttcatgt
      181 cgattccgga ggcggctcag ctcgtgatcc aggcgggaac gatggcgacc ggcggggaag
      241 tgttcgtgct ggagatgggg acgccggtca agatcgacga cctcgcccgc accatgatcc
      301 gtctctcggg tctcgaagtg cgcgacgaga aacaccccga cggcgacatc gccatcgcct
      361 atatcggcct caggcccggc gagaagctct atgaggagct tctgatcggc gagaacacga
      421 ccggcaccag ccatccgcgc atcttcaaga attccgagcc gttcgtgccg ttcgaggagc
      481 tcgccgcggt gctggaacgg ctcgacgacg cgatccagcg tttggacgag gccgagatgc
      541 aggagctgtt gcggtcggtg gtcgagggct atgtgccggc gagcagcgag ccgcagatcg
      601 cgggcaaggg cgcgtgggag cccgcctcgc gcacgctcca ctaagaggaa tgacggagcc
      661 tcagcctgtg ttgtggggcg cgattctggt cgcggggtgc gcgggcctct cggcattcct
      721 gatctttctg ttgaggccct tctttgtgcg ccatctcatg gcgcacccga acgcgcgctc
      781 atcgcacaag atcccgaccc cgcaaggcgc gggccttgct gtcatgctgt cggtcttggc
      841 gggctcggcg ctcgcgatcc tgatttcggg accggaagcc gagccgagcc tcattcccgt
      901 ccttggcatc gcagccggcc ttacgattct cggcgggatc gacgacgcgc tgacgctcct
      961 cgtgtcatgg cgccttctcg gccacgcgct tgcggccgta gcgctcgtgc tcagcctccc
     1021 tgaaggtttc cagctcttcc ccggcctcat gccgctcgtg gtcgaacgcg cgctgatcgc
     1081 gttcggcacg gtgtggttca tcaacgcggt caatttcctc gatggactgg attggatgac
     1141 cgtggtccag gtcgtgccga tcgcgctcgg gatcgccatc ctggaggcgc tcggcgccgt
     1201 gcccgccacc atcggggtgc tcgcgctcgc gctccttggc gccacgctcg gctttgcgat
     1261 cttcaacaag cacccggcgc ggatctttct cggcgacgcc ggaagtctcc cgatcgggct
     1321 ctgcctcgcc ttcatgctga tcttcgtggc gaaagcccat ctcgccgcgg cgctgcttct
     1381 gcccctctat acgatggcag acgcgaccct cactttggcc cggcgcatgc ttgccggaga
     1441 gcggatctct tccgcgcacc ggacgcattt ctatcagcgt ggcgtcgccg cgggcctcag
     1501 cgtgccgcaa gtgacggcgc gtgtgctgct gctgcaagtt ctgctcgtgg gtctcgcgat
     1561 tttaacggtg ctcgcccgct cgttgacggt cgacatcctc tgcctcgggc ttggcttgct
     1621 cgccaccggc ttcgcgctgt tcgctcttgc caaaggccgc tgacatgaag gggcgcgtgc
     1681 tcgtgaccgg cgcttccggg ttcatcggca agcgcttgac caccgctctt gcgcgggccg
     1741 gatgggaggt gagggccgcc gcccgcgatc ccgccggcgt ccccaccgca agcggggtcg
     1801 agcggatcgc gatggacgat cttgccagag gctgcgattg gccggcgctg ctcgagggcg
     1861 tcacccatgt cgctcatctc gccggcatcg cgcatgcgcc gggcaccctc cccgaggcgc
     1921 tgtacgcccg catcaatgcg gagagcgtgg gcgaattcgc ggacgccgcg cgcggcaaga
     1981 tcgcgcgttt cgtgttcctg tcttcggtgc gggcgcaagc ggggctttcg gccgaccgcc
     2041 ccatcaccga gaaagacata cccgccccca cggacgccta tgggcgcgcc aagctcgaag
     2101 gcgagcggct cctcgccgaa agcggcgtcc ccttcacggt gctccgtccc gccgtggtct
     2161 acggcaaagg ggtgaaaggc aatatcgcgg ctcttgccac gcttgcgcgc acccccatgc
     2221 cgcttccatt cgcgggcttg agcaatcgcc gttcgctgct cgctctcgac aatctcgtct
     2281 cggcgatatc gctcgtgctc gacgcgcgcg aggccgcgaa cgagacattc gttgtcgccg
     2341 atgccgagcc gatcagcgtg gcgaacctgg tcgccgccat gcgggaaggt cttggacgct
     2401 cccctcacct cgtcaaggta ccgctcggag gggtcaagcg gctgatgcgg tctttcggca
     2461 aggaggcgga gtgggagcga atctcgggca atttcgtcgt cgatgcctcc aagctcaggg
     2521 gaatcggctg gcgccccgca attgcgactc gggacgggat cgccaaaatg atgcgttccg
     2581 agaacggcac cggcggctag actggcacat gcgacatctc tcatgctgag gagagtcttg
     2641 aggtccgttt cgaagcacgg cttacacaac tcgtcatgcc cggcttcatg ccgggcatcc
     2701 acgagttgca aagcgataat cgtggatggc cgggccaagc ccggccatga cgggaaacga
     2761 gggcgcgccg agctagctgg gtatgagcga atcgtagaga gcgacggtct cgcgcccgat
     2821 cgcctcggcc gagaacttgc tttcggcgag acggcgcgca ttggcgccga aacgctttcg
     2881 caattcgggg tcgccagcga gcctcgccat ggcgtgggcc aaggctttcg catcgccgac
     2941 gggcacggtc accgcggtct cgcctgcaat ggcgatctcg cggcagccgg gcgcgtcggt
     3001 cgcgaccatc gcgcggccgc aagcggccgc ttcgagcagg ctcttcggca ggccctcgcg
     3061 acgggaggcc agcacggcga agtgggcgcg ggcccagacc ttcctgacgt cggcgacatg
     3121 gccgagccac tcgatgccgt agaggctcgc gaactcgcgg agctgctcgg gcgcgagcga
     3181 tcccggattc tccctgtcgc aatctccggc aaggagcagt ttgagcgccg tcccgcgctc
     3241 ggcgaggagc gagaaggcct cgatcagcgt cagcacgccc ttgtcggcga gcatgcgtcc
     3301 gacataggcg gcggtgacgg gggccggcgg ctcgggcagc ggcgtcaaat gattggtatc
     3361 gacgccggag cccggaatga gcaccatggt ctcggggggc gcgccaagcg cgacgacgaa
     3421 agcgcgatcg tcggggttct ggacaatggt gcggcttcgc ttgcggcgga gaagacggga
     3481 cagagcaagc cgcacggcct gtcgcgccga ctcctttacg ttcgcctcgc caataaagag
     3541 cgtgcccaac ccagtcacgc tgttgaccac ggcggtcgcg gaaagaccga ggctcgcgac
     3601 cgtgccgagc agaaccggct tgagcgagac gttgtgcagg atggcgggcc gcaagtctcg
     3661 caagagacgc cgaagcgccc agatggccga gaggctcccc cgggctgaga ggctgccccg
     3721 gctccaggcg agagcgtgcg gcacgaagcc ttccctctcg atggccgcct tgccgtcgtt
     3781 cagccttgtc gcgacatgca cctcatagcc cgcagccttg gccgcgcgcg ccatgggcag
     3841 acgatgcgac aagaaatacc agtcctcggt gacgacgtag aggatgcgac gggccatcgg
     3901 ccaaggcttt tatcagcgca tccggggttg tggaacagag ggggccgcgg ccatctttcc
     3961 ccaatcctgc cgtctttgcg cccttcctct tgcggcgatt acgcgctatc ttctgcttcg
     4021 gctgacggaa gagcggcttt cgggggatgt catgaaatat ctcggattgg tgttgatctg
     4081 ttgcgccttt gcggtccaac ccgcgcaggc cttcgagatt cagggcgaaa aggcgacgct
     4141 cgaggatggc ggagcccagc tcacggctcc ggccaaccag ttcttgacgc cggacctttc
     4201 caagggcagc tcgcttgcgc tcccctatat cggtgagacc gacagcgggc tcatttcgga
     4261 ttacggcaac gccatttcga ttcccgcgcc cggcgtcgac aagccggcgc ctgcttgggc
     4321 cttgacgccc tcacgctagg ctcgaggatc gacatcgagc cccatctgtt ccacgaggaa
     4381 cgcgcgggtc gtgccaccct ttgggcagct tcgagaggag tttgtctttg ccggtttcgt
     4441 tcggggagag tgaataggag gtatgaatgc ataaggtcag aattgctgtt gcactcgtcg
     4501 ccgctctgct cgtcgcgggc ctggtgtcgt ggaccgcgga agccacgacg gcggccggaa
     4561 ccgcgggact gcccgagacg atcaagaaat attcgctgat cgagaaggcg gcctgcggcg
     4621 gatacggcaa gcattgtccg ccgggctaca cctgggtgtg caggccgaac cggtgcttct
     4681 gcgcccgctg ctaggggcac gttcatctct gaaaattgcc agggcggcag aggcatggac
     4741 ccctgccgcc cttaagtttt gatctgtggc tggccgcgcg cttgagagct ggtaccgccg
     4801 ggtgaattcg aatcaccgac ctcttgatcc acagtcagaa aagtcgccct gacaatggtg
     4861 gacacccttt gacacagacc accggggggc attgtaaacc agcaattctc ctgctaccta
     4921 ctggacaccg ctggacatta agcgcacttc caagcgcaac cttttgcaac ctatgccgcg
     4981 actcactgac aagctactga gggggctgaa gcctcctaag tctggacaga tagacgtttg
     5041 ggacgagctt cttccgagct ttggagtgcg aatcggagtt agtggccaca agtcattctt
     5101 cgtcggcacc agggtcaacg gaaggtaccg gcgtatcacg ctgcgtccgg cttatgacgc
     5161 gctctctcta gccgatgcga ggtcgcaagc acgcaagatc attgccgatg cacatgctgg
     5221 tgttggcccc gaggtaagaa ggcagcgcga agcaaaaagc accttcggag ccgtggctac
     5281 cgccttcatg caggacttcg cccacagtca ccgcacccgg ggggagatgc agcgcaagat
     5341 cgatgttgac ctcgttgatt ggcgcgatcg gcccatcgcc gacatcaagc gcggggacat
     5401 caaagaacta atccgcctga aggctagaac ggcacccatc gcggcaaacc ggctgctctc
     5461 gctcatcacc aagattttca attgggctct aaaggaggag ctgatcgaag cttctccggc
     5521 aatgcagatt gaccgtcccg ggaaggagac tgaccgcgag cgattcctgt cggcagacga
     5581 aatcagaatt gcatgggccg cattcgataa gctcggatac ccgtggggct cgctatacaa
     5641 gatgctcctc gttaccgggc aacggcgcgg cgaagttgcc ggcatgcggt ggggcgaaat
     5701 ctccccggac ggatggacgc taccaggagg gcgagccaag aagggtcagg ggcacctggt
     5761 cccgttatca actttggcgc gggaaattct cgatgacctc cccgagctag gtaaacttgt
     5821 ttttagatca cgcactgaca taccgctcgc aggctggagc aagactgcga agcggcttca
     5881 atcgttacag atgcagccgt ggcacctgca cgacctgcgc aggaccttcg ccactcacct
     5941 acgcaccctg ggcgtcgata ggctagttgt cagcaagctc ctcaaccatg ccgagggcgg
     6001 ggtgactaag acctatgacc gctgggccgc tgatctcgag aaggcggatg cgatggaacg
     6061 ttgggccatt cgcttgcggg agatcgttgg gctgtagttt tttctattcc cgatgggact
     6121 gctaacagga aacgagactc cgctggtcct gaaggaaacg atgcggtcaa gcatgctttc
     6181 tgcctagcgg cggtaagcac gtcgtcgcca gccaacctct tcgacagatg caacctgagc
     6241 cgttgcagcg gctattcctt agattgaggg gctgctgtgg cgggcactag ggtcatccac
     6301 acagcgcccg caaacatggc ggcgatgaac atcacagtac gcatgacttg acctcactga
     6361 gttgtctcgg gacgcctcca attcctcagc gccctgtcta actatctctc ggaaacggct
     6421 tttgctgaat gatcctttgt aagaggcgtt ccaacacctc aactctggcc gtcagcattt
     6481 ttaggctgcc tgctaaaagc cttcagctct gtctcactta tagtttcatt taactaggac
     6541 ctcggagacc tagcaatcaa gctgttcaat gttaatggcc gttgacggtg aatccacaca
     6601 acctcaattc caggccaaaa actccacacg attcggtgag gtttcagtcg cttatttcac
     6661 cggccctagg aatccaggct gtagcgggct gcagcgctga gcgaagcgca cagcttgcgg
     6721 cgaggcgttg aaggaccgcg gttccgcgga gccaatagac tccggcatgg tcggagccca
     6781 ggccattgcg gccgacccgg agagaggact cctgacaagc tgcaagaact ttggaccgcg
     6841 ctcggggcgg cgggattctc gaacaacgag ctcagcttgt aagatcattg gaat
//
LOCUS       DASUQP010000191         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2004455, whole genome shotgun sequence.
ACCESSION   DASUQP010000191 DASUQP010000000
VERSION     DASUQP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004455"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFY74_08535"
     CDS             <1..242
                     /locus_tag="VFY74_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HEX5931776.1"
                     /translation="LSLYVDLHGTGSVSEDKLEQALRQVMDLSPRGIREHLKLSRPIY
                     ERTAAYGHFGRQPDNDGGFSWERLDLAEPIRAELS"
     gene            336..1046
                     /locus_tag="VFY74_08540"
     CDS             336..1046
                     /locus_tag="VFY74_08540"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEX5931777.1"
                     /translation="MIVGRKAEISAPAPLRSYGRRKAKPLSARKERLIGEFLPLIRVD
                     LAQEAPRSLSALYPVPVKDVWLEIGFGSGEHLLWQAERHQDVGFIGCEPFINGMGSLL
                     GAIEERGIGTILVHDGDAREVLAWLPDRSIGRIFLLFPDPWPKRRQQKRRLLARETVS
                     ELARVLVPGGELRFASDSGDYAAQALLLARTSGAFVWRAVRPHHWRERASDWPETRYE
                     RKALGEGRKAVYLTFGRL"
     gene            1194..1751
                     /gene="rimP"
                     /locus_tag="VFY74_08545"
     CDS             1194..1751
                     /gene="rimP"
                     /locus_tag="VFY74_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX5931778.1"
                     /translation="MSSPTGNADRRFARETGPARIIADLAEPVLDELGFRLVRVKISG
                     RDGGTVQIMAERAGGGMSVEDCAAISRALSPVLDAYDPMPGQYRLEVSSPGIDRPLVR
                     PSDFALWAGHEAKVELKELLDGRKRFRGVIEGIAKDEVRLRIELDGKADPVVIGLPFA
                     LIAEAKLVADLAGFRADLSAKKPAH"
     gene            1819..>2184
                     /locus_tag="VFY74_08550"
     CDS             1819..>2184
                     /locus_tag="VFY74_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX5931779.1"
                     /translation="MAQAGVSANRLELLQIADAVAREKSIDRQVVITAMEDAIQKAAK
                     SRYGSENEIKAEVDPKTGEIRLARLLEVVETVTMEATQVSLDEARRRNPAAQVGDFIA
                     EPLPPLDFGRVAAQNAKQVI"
BASE COUNT          390 a          675 c          742 g          377 t
ORIGIN      
        1 cgctctcgct ctatgtcgac ctgcacggca cgggcagcgt ctccgaggat aagttggagc
       61 aggcgttgcg gcaggtcatg gacttaagtc cgcgcgggat cagggagcat ttgaagctga
      121 gccgcccgat ctacgagcgg accgcagcct acggccattt cgggcgacag cccgacaatg
      181 acggcggctt ttcctgggag cggctcgatc tcgccgagcc gatccgcgca gagctgtcct
      241 gataggccgc cgccgccttg ctttggttcg ccgatgcggc aagcctgacc cttcaaaact
      301 atggatgcct ggttcaagtc ggagcatgaa ggggaatgat cgtcggtcgg aaggcagaga
      361 taagcgcgcc cgcgccgctc aggtcttatg gcaggcgtaa agccaagcct ctgagcgcgc
      421 ggaaggagcg cctgatcggc gagttcctgc cgctcatccg cgtcgatctc gcgcaggaag
      481 cgccgaggtc cctttcggca ctctatcccg tgccggtcaa ggacgtctgg cttgagatcg
      541 gcttcggttc gggtgagcat ctgctttggc aggccgagcg gcatcaagat gtcggcttca
      601 tcggttgcga gcccttcatc aacggcatgg gaagcctcct cggcgccatc gaagagcgcg
      661 gcatcgggac gatccttgtg catgacgggg acgcgcggga ggtgctggcc tggttgcccg
      721 accgctccat cggccgcatc ttcctgctct tccccgatcc ttggcctaag aggcgccagc
      781 agaaacggcg gctcttggct cgcgaaacgg tgagcgagct cgcccgcgtg ctcgtgccgg
      841 gcggcgaact tcgatttgcc tcagacagcg gcgactacgc cgcccaggcg cttcttctcg
      901 cgcgcacgag cggggccttt gtctggcggg cagtgcggcc gcaccattgg cgcgagcgcg
      961 cttcagactg gccggaaacg cgctacgagc gaaaagcgct gggcgaaggg cggaaagccg
     1021 tttacctcac tttcggccgc ctctagccct catggccgcg ccgcgcaggg cgcttgaaag
     1081 cgcagagaaa gctcgtatat taagcccgaa ctcatgattg attgtgatca ggagtgggcc
     1141 ttcgcccgct cctttttatt aggctcaacg ctcttttgcg gccgaagcgc ccgatgtcga
     1201 gccctaccgg caatgccgat cgacgattcg cccgcgaaac gggacccgcg cggatcatcg
     1261 ccgatctggc ggagcccgtg cttgacgaac tcggcttccg gctggtcagg gtcaagatct
     1321 cggggcgaga cggaggcacg gtgcagatca tggccgagcg ggcaggcggc gggatgagcg
     1381 tggaggattg cgccgcgatc agccgggcct tgtcaccggt tctcgacgct tacgacccta
     1441 tgcccgggca gtatcgtctc gaggtctcct ctccggggat cgaccggccg ctggtgagac
     1501 cgagcgactt tgcgctatgg gccggccacg aagcgaaggt cgagctgaaa gagctgcttg
     1561 acgggcggaa gcgcttcagg ggcgtgatcg aagggatcgc caaggatgag gtgcggctca
     1621 ggatcgagct cgacggcaag gccgaccccg tggtcatcgg ccttcccttc gcgctcatcg
     1681 cggaggcgaa gctcgtcgcg gatcttgcag gcttcagagc agacctttcg gccaagaagc
     1741 ccgctcattg aatgaacgga gcgtccccgc ttctggcggc gcgcttccgg gaagacggaa
     1801 acgattggag gaatagagat ggctcaggca ggcgtcagcg ccaacaggct cgagctcttg
     1861 cagatcgcgg acgcggtcgc gcgcgagaag tcgatcgatc gccaggtggt catcaccgcc
     1921 atggaggacg cgatccagaa ggcggcgaag tcgcgctacg gcagcgagaa cgagatcaag
     1981 gccgaggtcg atcccaagac cggcgagatc aggctcgcgc gcctgctcga agtggtcgag
     2041 accgtcacca tggaggcgac ccaggtttcg ctcgacgagg cgcggcgccg caatccggcg
     2101 gcgcaagttg gcgacttcat cgccgagccc ctgcccccgc tcgatttcgg ccgggtcgcg
     2161 gcgcagaacg caaagcaggt gatc
//
LOCUS       DASUQP010000192        10815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1185805, whole genome shotgun sequence.
ACCESSION   DASUQP010000192 DASUQP010000000
VERSION     DASUQP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185805"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFY74_08555"
     CDS             complement(<1..340)
                     /locus_tag="VFY74_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX5931780.1"
                     /translation="MPLSQSVEGCLEAKIGRAGLPQASLDANLAKLEPRLASLREAYA
                     NSTLPLLKVPEWRDDIAPAREALRRLTEGARTLIFFGTGGSSLGGQTLAQLGGWGIPG
                     DDKHGSAERPR"
     gene            complement(390..1652)
                     /locus_tag="VFY74_08560"
     CDS             complement(390..1652)
                     /locus_tag="VFY74_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5931781.1"
                     /translation="MKIQRVTSPGGIEAWLVESHANPLIAMRFAFRGGAAQDTEDKEG
                     LAYFISAMMDEGAGPLDAIAFQEREQALAMRMDFDASRDVMLGSVQTLTANKDEVFDL
                     LRLALTEPRFDQDAVERVRRQILAGLKFDENDPESVASLAWDRLAFRDHPYGRPIKGT
                     MTSVATISPSDLRDYADRVFARDKLVISVVGDIDADSLGKVLDHVFGGLPALSALEPV
                     ADANPPLGPSREIIEMDVPQSVVQFGHRGIARKDDDFMPAYVLNYILGGGGFASRLME
                     EVREKRGLAYSVYSNLYPYQHGAVFIGNVATKNEAVGQSLDVIESELKRLAEEGPTAE
                     ELANARSYLTGAYALRFESSSGIANQLLWIQIEDLGIDYVDRRNALVEAVTLDDIRRV
                     AKRLIEADHLITTIVGKPVAAKAAEVPG"
     gene            complement(1766..3205)
                     /locus_tag="VFY74_08565"
     CDS             complement(1766..3205)
                     /locus_tag="VFY74_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5931782.1"
                     /translation="MMMWIFEQLLPAVDGRMGFFVASFSLASLAVAAAAEKPQASPRV
                     SEFKLANGLQVLVIPDRRAPVVTQMIWYKVGAADEPRGSSGIAHFLEHLMFKGTDAIP
                     LGQFSKIIARNGGEDNAFTNHDVTAYFQRVAKDRLFKVMEMEADRMANLRLSEEDVTT
                     ERNVILEERRSRVDNDPGSILQEQMMAALYANHPYGIPIIGWEHEIRALDRDDALSFY
                     RRFYAPNNAILVIAGDVDPEEVRRLAEATYGKIAPNDAANGRARPQEPPRFSPVKVTL
                     EDARAGRITVQRYYMAPSYASAEPGEAEALDLLMRVAAAGSVSRIYKRLVIEDKKAAN
                     AGGWYSDSGLDSGRLGFYSVAAEGVTADELDKAIGGVIAELREEGVTQEELDRARASY
                     IADFVYTSDSQSRMARHYGWRLATGMTVADVEEWPDRLKRVTVEGMRDVARKYFVDKN
                     SVTGYLLPAPAHTSSVGGKPVHAPVKGKS"
     gene            complement(3355..3918)
                     /locus_tag="VFY74_08570"
     CDS             complement(3355..3918)
                     /locus_tag="VFY74_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931783.1"
                     /translation="MIGPARAALLAAVSAATLALAGCGAIEFQGKIFDYMGVSGDRKE
                     PDVHMAERPPLLLPPDPKALPQPQNGVAAATARQDWPQNPEVTQKQIIQAEKDEKAKE
                     RRIQEPLNPYIGKKTLLDKWLTRNKEGPPVEDVPEPDPSDKAPEDVAAAKPKPPLKPH
                     VPQDITPTEDPFNPPPPDSYKNPSALY"
     gene            complement(3970..4485)
                     /gene="lspA"
                     /locus_tag="VFY74_08575"
     CDS             complement(3970..4485)
                     /gene="lspA"
                     /locus_tag="VFY74_08575"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5931784.1"
                     /translation="MLRDWLWGPLSPLGLGIAALTVLVDQAHKAWMLYVYDIGAKGVV
                     TITPFFDLVLVWNQGISYGLFPQESGLGRWGLIVFAFAASLAFAVWLARCSAPLVAAS
                     IGLIIGGAVGNAIDRMAYGAVADFFSLHVFGFQWYVFNIADAAIVAGVVGLLYESLFR
                     GHKKVGNPSKM"
     gene            complement(4490..7843)
                     /gene="ileS"
                     /locus_tag="VFY74_08580"
     CDS             complement(4490..7843)
                     /gene="ileS"
                     /locus_tag="VFY74_08580"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5931785.1"
                     /translation="MARNTKGKRTAARGAGARQARGKVNAKRKTLQRTPKRAALKGKP
                     ARASAKRKSVKTKTDTAKSAKRETETKARAKPKAKAAPKRGVEPGRAGAKAIVSRTVE
                     RAPLARDPRSLIARLEDQASKIETGRDWSETLFLPKTDFPMKAGLPEREPELLARWER
                     LGLYDRLREDAQGREAFVLHDGPPYANGHIHIGTGLNKILKDVVVRSQQMMGKDANFV
                     PGFDCHGLPVEWKVEEENYRARGKTKPDLSNPEAMVAFRAECRAYAEHWLGVQREEFK
                     RLGVVGDWKHPYTTMAFDAEATIAAELMKFAMNGLLYRGSKPVMWSVVERTALAEAEV
                     EYEEVESPSIYVKFPVLAVGPSGNTGLSPDEIKSAAVVVWTTTPWTIPGNRAISFSKE
                     IDYGVYEVTQAPDDNWAKVGDRLILADKLAEDVFAVARVSAWRRLGTMPWQALEALTA
                     AHPLRGRGYEFDVPLLPGPHVTEDTGTGFVHTAPGHGTEDFEVFEEHRGDLEEHGIDT
                     KIPFTVDEAGFFTKEAPGFEGKRVIDDKGRFGDANLAVIAALTEAGALIARARRKHDY
                     PHSWRSKKPVIFRATPQWFIALDSPAALSIPARSGQGTLRARALKAIGETRWVPPQGE
                     NRITGMIETRPDWVVSRQRAWGVPITVFRHKETGEVIPRVDFEGSVKLIERIFSAFRG
                     EGADAWFKRGAKERFLTGLVDNPADWDKADDILDVWFDSGSTHAFVLEKRLDLEWPAA
                     LYLEGSDQHRGWFQSSLLESCGTRGRAPYDAVVTHGFVVDEDGRKMSKSLGNVVAPQD
                     VIRQSGAEILRLWAMSSDSAEDLRIGPDILKANIESYRKLRNTLRFLLGNLAHFRPDL
                     AVRHADMPGLERYMLSRLAELGETVRTGYDAFDFKRVFHALLNFCVNDLSAFYFDIRK
                     DALYCDPYDSLTRRAALTLLDHLFEALTAWLAPMLCFTMEEAWLNRFPGDRGSVHLRQ
                     FPEIPHAWRDEQLAAQWRKIRDLRRVVTGALEIERKEGRIGSSLEASPKVYVADADLR
                     AALDGVDLAEIAITSGAELLPGAGPAHAFRLDDVKGVAVVFEPARGRKCARSWKILEE
                     VGSDPDFPALSLRDAAAVRQFDARRAGAR"
     gene            complement(7935..8927)
                     /locus_tag="VFY74_08585"
     CDS             complement(7935..8927)
                     /locus_tag="VFY74_08585"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX5931786.1"
                     /translation="MEIVRVWRDVPKKLKGAALAIGNFDGVHRGHQRVLGEAIAIAGR
                     EGRRSGAVVFEPHPREFFAPETPFFRLTPLPLKLELLEALGLDQTFVIGFDRELASLS
                     APEFAIEVVAQGLGATHVVVGHDFSYGKGRTGTTEELAALGRAQGFGVDVVAPVGESG
                     TTFSSSRIREHLRNGEAREAAEQLGYWWRVRGAVGAGAGRGKGLGFPTLNLPLAQGQD
                     VRHGIYAMRVEHDGRRYHAAGYVGARPTFGAGEPVLEAYLLDFAGDLYGKVVDVEFIA
                     FLRPDQTFATPEALAARMREDTDRARAILVEIDADDPIARFPLARALAKAAAAS"
     gene            complement(8948..9409)
                     /locus_tag="VFY74_08590"
     CDS             complement(8948..9409)
                     /locus_tag="VFY74_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5931787.1"
                     /translation="MTTNGNRAGGLGHGYYFEDLSVGMEANYTKKITDKDVLAFAELS
                     GDVNPVHISDDFAAATIFKKRIAHGFLTGSLFSTVLGTKLPGPGCIYLSQSMKFRAPV
                     YIGDEVVATLRITGLDIDKARATLACDCAVNGKIVLEGEAVMMVDRRTPRQ"
     gene            complement(9611..10498)
                     /locus_tag="VFY74_08595"
     CDS             complement(9611..10498)
                     /locus_tag="VFY74_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX5931788.1"
                     /translation="MTVAASDIPVLDSIKSLASHYRGWLVDIWGVMHNGHRAFPRAVA
                     ATRAFRATGGIVVLLSNSPRPSPSVMDQLRRLGVPDDSYDATVTSGDLTRHELVKHAG
                     ATVFHLGPLRDRPIFEGLDVKLGRHEDSELIVCSGLFDDETETPEDYSELLSALAARK
                     LPMICANPDQLVERGDQLVWCAGALAAVYEKAGGPVVYAGKPYAPIYMLALETIGALA
                     GRAVPKSEILAIGDGVHTDIAGAAGVGIDSVFVASGLHRPANSGGEAGGEALAAAHLD
                     ALFAHANPRPVGAMRALIW"
BASE COUNT         1823 a         3520 c         3566 g         1906 t
ORIGIN      
        1 tgcgcggccg ctcagcgctc ccatgcttgt cgtcgccggg aatgccccat ccgccgagct
       61 gggcgagcgt ctggccgccg aggctcgatc cgccggtgcc gaagaagatc agtgtccgtg
      121 cgccctcggt aagccgccgc agggcttcgc gcgccggcgc aatgtcgtcc cgccattccg
      181 gcaccttcag cagcggcaag gtggagttgg cataggcctc gcgcaaggag gcgaggcgcg
      241 gctcgagctt ggcgagattg gcgtcaagcg aggcttgcgg gagccccgcg cgcccgatct
      301 tcgcctcaag gcaaccttcc acggattgcg acagcggcac caaccagtcc ctttcgccca
      361 cgaagatcag atgtcggacg cgacgagccc tagcccggca cttccgcagc cttcgccgct
      421 acaggcttgc cgacgatggt ggtgataagg tgatcggcct cgatcaggcg cttggccacg
      481 cgtctaatgt cgtcaagggt cacagcctcg accagcgcgt tccgccgatc gacgtagtcg
      541 atgccgagat cttcgatctg gatccagagg agctggttgg cgatgcccga cgaggattcg
      601 aacctgagcg cgtaggcgcc ggtgagatag ctgcgcgcgt tcgcgagctc ctcggccgtg
      661 gggccctcct cggcgagccg cttcagctcg ctctcgatca cgtcgagcga ctggccgacg
      721 gcttcgttct tggtggccac gttgccgatg aagacggcgc cgtgctgata gggatagaga
      781 ttggagtaga ccgaataggc gagcccccgc ttctcgcgca cctcctccat caggcgtgag
      841 gcgaagccgc cgccccccag gatatagttc agcacgtagg ccggcatgaa atcgtcgtcc
      901 ttccgcgcga ttccgcgatg gccgaactgc accaccgatt gcggcacgtc catctcgatg
      961 atctcgcgcg agggcccaag cggcggattg gcgtcggcca ccggctccaa tgccgaaagc
     1021 gccggcaagc ccccgaacac atgatcgagc accttgccga ggctatcggc gtcgatgtcg
     1081 ccgacgacag aaatgacgag cttgtcgcgc gcgaagacgc ggtcggcata gtctctgagg
     1141 tccgagggcg aaatcgtcgc gaccgacgtc atcgtgccct tgatcggccg cccgtaaggg
     1201 tgatcccgaa aggccagacg atcccaggcg agcgaggcca cgctctcggg gtcgttctcg
     1261 tcgaatttga gcccggccag aatctgcctg cgcacgcgtt cgaccgcgtc ctgatcgaaa
     1321 cgcggctcgg tcagcgcaag ccgcaggaga tcaaacacct cgtccttgtt cgcggtcagc
     1381 gtctggacgc tgccgagcat gacatcccgg ctcgcgtcga agtccatgcg catggcgagc
     1441 gcctgctcgc gttcctgaaa ggcgatcgcg tcgagcggcc ctgcgccctc gtccatcatg
     1501 gcggaaatga aataggcgag accttcctta tcttccgtgt cctgcgcggc accgccgcgg
     1561 aaggcgaagc gcatggcaat cagtggattg gcgtggctct cgacgagcca tgcctcgatg
     1621 ccgccggggc tggtcacgcg ttggatcttc atgccggtaa cttcagccgc cgatgcggcc
     1681 gaaacggcga cgaccgcggc agcggcaaag cccgcgccgt gccgcaccag ggagcgaagg
     1741 aaggaacccc cccgcgccat cgcggctacg acttgccttt gacgggcgca tggaccggct
     1801 ttccaccgac ggagcttgta tgtgcggggg caggcagcag atagccggtg accgaattct
     1861 tgtcgacgaa atatttgcgc gcaacgtccc gcatgccctc gacggtcacg cgcttcagcc
     1921 ggtcaggcca ttcctcgacg tcggcgacgg tcatgccggt ggcaagccgc cagccatagt
     1981 ggcgcgccat gcgcgactgg ctgtcggagg tgtagacgaa gtcggcgata taggaggcgc
     2041 gcgcccggtc gagctcttct tgcgtcacgc cctcttcgcg cagctctgcg atcacgccgc
     2101 cgatggcctt gtcgagctcg tcggcggtga cgccctcggc ggcgacggaa tagaagccga
     2161 gccggcccga atcgaggccc gaatccgaat accagccgcc ggcgttggcc gcctttttgt
     2221 cctcgatcac caggcgctta tagatcctgc tgacggagcc cgcggcggcg acgcgcatca
     2281 ggaggtcgag cgcctcggcc tcgccaggct cggccgacgc gtagctcggc gccatgtagt
     2341 agcgttgcac cgtgatgcgt ccggcgcgcg catcctcgag cgtcaccttc accggagaaa
     2401 agcgcggagg ctcctgcggc cgggcgcggc cgttcgctgc gtcgttcggc gcgatcttgc
     2461 cgtaggtcgc ctcggcgagc cgcctcacct cctcgggatc gacgtcgccc gcgatcacca
     2521 gaatcgcgtt gttcggcgcg tagaagcgcc gatagaacga gagcgcatcg tcgcgatcga
     2581 gcgccctgat ctcgtgttcc caaccgatga tggggatgcc atagggatga ttggcgtaga
     2641 gcgccgccat catctgctcc tgcaagatac tcccgggatc gttgtcgacc cggctgcggc
     2701 gctcttccag gatcacattg cgctcggtgg tcacgtcctc ctcggagagg cgcaagttcg
     2761 ccatgcggtc agcttccatc tccatcacct tgaacaggcg atccttggcc acgcgttgga
     2821 aataggcggt cacgtcatga ttggtgaagg cgttgtcctc gccgccgttc ctggcgatga
     2881 tcttcgagaa ttgacccagg ggaatggcgt cggtgccctt gaacatgagg tgctcgagaa
     2941 aatgcgcgat gccggaggat ccccgcggct cgtccgcagc gcccaccttg taccagatca
     3001 tctgggtgac gaccggcgcg cggcgatcgg gaatgacgag cacctggagc ccgttcgcga
     3061 gcttgaactc gctcacgcgc ggtgaggctt gcggcttctc cgccgcggcg gcgacggcga
     3121 gcgaagcaag gctgaacgac gcaacgaaga agcccatccg gccgtcgacc gccgggagaa
     3181 gctgttcgaa tatccacatc atcattgtgc gagaccggaa aagatcgatg caaggccgcc
     3241 gggcaagaat tcgtgccgaa tcttggcggc cagcccttct cccatttcag gccttggacc
     3301 gccattttca agcgaattcg gctggagacg cgcgccaaaa cccccaggtt tccgtcagta
     3361 aagggcgctt gggttcttgt agctatcggg cggcggcggg ttgaacgggt cctcggtcgg
     3421 cgtgatgtcc tgcgggacgt gcggcttgag cggcggcttc ggctttgcgg cagcgacgtc
     3481 ctcgggcgcc ttgtcggaag gatcgggctc gggaacgtct tcgaccggcg gaccttcctt
     3541 gttcctggtg agccatttat ccaagagggt cttcttgccg atgtaaggat tgaggggctc
     3601 ttggatgcgc ctttccttgg ccttctcgtc cttctctgcc tggatgatct gcttctgcgt
     3661 cacttcgggg ttctgcggcc agtcttggcg cgcggtggcc gcggcaacgc cgttctgcgg
     3721 ctggggaagc gccttggggt cgggcgggag cagaagcggc ggccgttcag ccatgtggac
     3781 gtcgggctcc ttacggtcgc ccgaaacccc catgtagtcg aagatcttgc cttggaactc
     3841 gatggcgccg cagcctgcga gggcaagcgt ggcggcagac accgcggcaa ggagcgcggc
     3901 ccttgcgggg ccgatcatgt aaaagctgtg ccccaaggtt ccccctcaag cccggaaatc
     3961 cgggattccc tacatcttag aagggtttcc gacctttttg tgacccctga acaaggactc
     4021 atacagaaga ccgacgaccc cggcaacaat ggcggcgtcg gcgatgttga acacgtacca
     4081 ttggaagccg aacacgtgga gggagaagaa atcggcgacc gcgccgtagg ccatgcgatc
     4141 gatggcattg ccgacggcgc cgccgatgat aaggccgatc gaagctgcca cgagaggcgc
     4201 gctgcatcgt gccagccaaa cggcgaaagc gaggcttgcg gcgaaggcga acacgatcaa
     4261 gccccagcgg ccgagaccgc tctcttgcgg aaacagtcca tagctgatgc cttgattcca
     4321 cacgagcacg agatcgaaaa agggcgtgat ggtcaccacg cccttggcgc caatgtcata
     4381 gacatagagc atccacgcct tgtgggcctg atcgacgaga acggtcagag cggcgatgcc
     4441 gagcccgagc ggcgacaggg gaccccacaa ccagtcgcgc agcatcgcgc tacctcgcgc
     4501 ccgcgcggcg tgcgtcgaat tgcctgaccg ctgccgcatc gcgcaaggac agcgcgggaa
     4561 aatcgggatc ggagccgacc tcctcgagga tcttccagga tcgggcgcat tttctgcccc
     4621 tggcgggctc gaacacgacc gccacgccct tcacatcatc gagacggaag gcgtgggcgg
     4681 gccccgcgcc cggcaacagc tcagccccgc tggtgatcgc gatctcggcg agatcgacgc
     4741 cgtcgagagc ggcgcggagg tcggcgtcgg cgacgtagac cttcggagac gcctccaggc
     4801 tcgagccgat gcgcccctct ttcctttcga tctcgagcgc gcccgtgact acgcggcgca
     4861 agtcgcgaat tttccgccat tgggcggcga gctgttcgtc gcgccacgcg tgcgggattt
     4921 ccggaaattg acgcagatga accgagccgc gatcgccggg aaaccgattg agccatgctt
     4981 cttccatggt gaagcagagc atcggcgcaa gccacgccgt aagcgcctcg aacagatggt
     5041 cgagcaatgt caacgccgcg cggcgcgtca agctgtcata agggtcgcaa tagagcgcgt
     5101 ccttgcggat gtcgaaatag aaggcggaca gatcgttgac gcagaaattg aggagcgcat
     5161 ggaagacgcg cttgaagtcg aaggcgtcgt agcccgtgcg cacggtctcg ccgagttcgg
     5221 caagccgcga aagcatgtag cgctcgagcc cgggcatgtc ggcatgacgg acggcaaggt
     5281 cgggccggaa atgggcaaga ttgccgagaa ggaagcggag cgtgttccgg agcttccggt
     5341 acgattcgat attggccttg aggatgtcgg gtccgatgcg gagatcttcc gcagagtccg
     5401 agctcatcgc ccacagtctc aggatctcgg cgcctgactg gcggatcacg tcttgcggcg
     5461 ccaccacatt gccgagcgac ttcgacatct tgcgcccgtc ttcgtccacg acgaagccgt
     5521 gggtgacgac ggcgtcgtag ggcgcgcggc cgcgcgtccc gcaagattcg agcagcgacg
     5581 attggaacca gccgcggtgc tggtccgacc cctcgagata gagcgcggcg ggccattcca
     5641 gatcgagccg cttctccaga acgaaggcat gcgtcgaacc cgaatcgaac cagacatcga
     5701 gaatgtcgtc ggccttgtcc caatcggcgg ggttgtcgac gaggccggtc aggaaacgtt
     5761 ccttcgcgcc gcgtttgaac caggcatcgg cgccttcgcc gcggaaggcc gagaagatgc
     5821 gctcgatcag cttgaccgat ccttcgaagt ccacgcgcgg gatgacctct cccgtctcct
     5881 tgtggcggaa tacggtgatg ggaacgcccc aggcacgctg gcgcgacacg acccagtcgg
     5941 gccgcgtctc gatcatgccg gtgatgcggt tttccccttg cggcggcacc cagcgcgtct
     6001 cgccgatcgc cttcaacgcg cgcgctctca gcgtgccctg ccccgagcgg gccggtatgg
     6061 acagcgcggc aggactgtcc agggcgatga accattgcgg cgtggcgcgg aagatcaccg
     6121 gcttcttcga gcgccacgaa tgcgggtagt cgtgcttgcg gcgcgcacgc gcaatcagcg
     6181 cgccggcttc cgtcagagcc gctatcacgg ccaggttggc atcgccgaac ctgcccttgt
     6241 cgtcgatgac gcgcttgccc tcgaagccgg gcgcctcctt ggtgaagaag ccggcctcgt
     6301 cgacggtgaa cgggatcttg gtgtcgatac cgtgctcttc gagatcgccg cgatgctctt
     6361 caaagacctc gaagtcttcg gtgccgtggc ccggcgcggt atggacgaag cccgtgcccg
     6421 tgtcctccgt gacatgaggt ccgggaagca gcggcacgtc gaactcgtag ccccggccgc
     6481 gcaagggatg ggccgcggtc agggcttcga gcgcttgcca tggcatagtg ccgagccgcc
     6541 gccacgcgct gacgcgcgcg acagcaaaca cgtcctcggc gagcttgtcg gccaggatca
     6601 gcctgtcgcc gaccttcgcc cagttgtcgt cgggcgcctg cgtgacctcg tagacgccat
     6661 agtcgatctc cttcgagaag gagatggcgc ggttaccggg aatggtccaa ggcgtcgtgg
     6721 tccagacgac gactgcggca ctcttgatct catcgggact gagaccggtg ttgccgctcg
     6781 gacccacggc gagcacggga aacttcacat agatcgacgg gctttcgacc tcctcatatt
     6841 cgacttccgc ctctgcgagc gccgtgcgct cgaccaccga ccacatgaca ggtttcgagc
     6901 cgcgatagag cagcccgttc atggcgaact tcatcagttc ggcggcgatg gtggcttccg
     6961 cgtcgaaggc catggtggtg tagggatgtt tccaatcgcc cacgacgccg agccgcttga
     7021 actcctcgcg ttgcaccccg agccagtgtt cggcgtaagc gcgacactcg gcacggaagg
     7081 cgaccatcgc ctcgggattt gacaggtcgg gcttcgtttt gccgcgcgcg cgatagttct
     7141 cctcctcgac cttccactcg acgggcagcc cgtgacagtc gaagcccggc acgaagttgg
     7201 cgtccttgcc catcatctgc tgcgagcgga cgaccacgtc cttcaggatt ttgttgaggc
     7261 ccgtgccgat atgaatgtgg ccgttggcgt aaggcggccc gtcatgaagc acgaaggctt
     7321 cgcgcccctg cgcgtcttcg cgcagcctgt cgtaaaggcc aagccgctcc cagcgcgcga
     7381 gaagttcggg ctcgcgctcg ggcagtccgg ccttcatcgg aaagtccgtc ttgggcagga
     7441 agagcgtctc gctccagtcg cgtccggtct cgattttcga cgcctggtct tcgagcctgg
     7501 cgataaggga tcggggatcg cgcgcgagcg gcgcgcgctc gacggtgcgc gaaactattg
     7561 ctttcgcccc ggctcgtccg ggctcgacgc cgcgcttcgg tgctgctttc gccttcggct
     7621 tcgctcgcgc tttggtctct gtctcgcgct tggcggattt cgccgtgtcg gtcttcgtct
     7681 tgaccgactt cctcttcgcc gacgctctcg ccggtttgcc cttgagggcg gcgcgtttcg
     7741 gcgttcgttg cagggtctta cgtttcgcgt tgacctttcc ccgggcttgg cgggccccgg
     7801 ctcccctagc ggccgttcgt ttgccctttg tgttccttgc catgttccct gcttctggag
     7861 tcggttccgc cggcttcgcc ccgcggcgtt ctagcaatcg cactccgctc atgccagggc
     7921 cttcgtgccc gttatcaact cgccgccgcc gccttggcga gcgcgcgggc gagagggaag
     7981 cgcgcgatcg ggtcgtcagc atcgatttcg acaaggatgg cgcgggcgcg atcggtgtcc
     8041 tcgcgcatcc tggccgcgag cgcttcgggc gtggcgaagg tctgatccgg acgcaggaag
     8101 gcgatgaact cgacatcgac caccttgccg tagaggtcgc ctgcaaaatc gagcagatag
     8161 gcttcgagca caggctcgcc cgcgccgaag gtggggcgcg cgccgacgta acccgcggcg
     8221 tgatagcggc gcccgtcgtg ctcgacgcgc atggcataga tgccgtgacg tacatcttgt
     8281 ccttgcgcga gcggaagatt gagggtcggg aagccgagcc ccttgccgcg cccggcgccc
     8341 gcgccgaccg caccgcgaac gcgccaccaa tagccgagct gttcggccgc ctctctcgct
     8401 tccccgttgc ggaggtgctc gcggattcgg ctcgacgaga aggtcgtgcc gctctcgccc
     8461 acgggtgcca ctacgtcgac gccgaagccc tgcgcgcgcc cgagcgctgc aagctcctcc
     8521 gtcgtgccgg tgcggccctt gccgtaggag aaatcgtggc ccacgaccac atgggtcgcg
     8581 ccgagccctt gcgccaccac ctcgatggcg aactcgggcg cgctcagcga agcaagctcc
     8641 cggtcgaagc cgatgacgaa tgtctggtcg aggccgagcg cttctagcag ctcgagcttc
     8701 aacggcagcg gcgtcagccg gaagaacggg gtctcggggg cgaagaattc cctcggatgc
     8761 ggttcgaaca ccacggcgcc cgagcgccgc ccttcgcgcc cggcaatcgc gatcgcctcg
     8821 ccgaggaccc gttgatggcc gcgatgcacg ccgtcgaaat tgccgatggc tagcgctgcg
     8881 cccttcagct ttttcggcac gtcccgccag acgcggacaa tctccatcgc ttgccttcga
     8941 tccccgccta ttggcgcggc gtccggcggt cgaccatcat caccgcctcg ccttcaagca
     9001 cgatcttgcc gttgaccgcg cagtcgcatg cgagcgtggc gcgggccttg tcgatgtcga
     9061 ggccggtgat gcggagcgtg gcaaccacct cgtcgccgat atagaccgga gcccggaact
     9121 tcatgctttg ggacagatag atgcagccgg gacccgggag cttggtcccg agcacggtgg
     9181 agaacagact gcccgtgagg aagccgtggg cgatccgctt cttgaagatg gtcgcggcgg
     9241 cgaaatcatc gctgatatgc acgggattga cgtcgcccga gagctcggca aaggcgagaa
     9301 cgtccttgtc ggtgatcttc ttcgtatagt tcgcctccat gccgacggag aggtcttcga
     9361 agtaataccc gtggcccagg ccgccggcgc ggtttccgtt cgtcgtcatc ttgcgttttc
     9421 ctgctccctg cgtttggcag ggggcaaaat agcgaaggcc ttgctgcgcc gcaatctcag
     9481 ggacgcgcaa ggcgccaaac cgcccctcgt catcctcagg cgcgagcctc gaaggatgcc
     9541 atccggtgtg ttgcgtcctt cgagggctgc ctccggcagc cacctcagga tgacggtgag
     9601 cggagcgagg ctaccagatg agcgcgcgca tggctccgac ggggcgcggg ttcgcgtgcg
     9661 cgaacagcgc gtcgagatgc gcggcggcga gggcctcccc gcccgcctcg ccgccgctgt
     9721 ttgccggcct atgcaagccg cttgcgacga acaccgaatc gatgccgacg cccgcggcgc
     9781 ccgcgatgtc ggtgtgcacg ccatcgccga tggcgagaat ttcgcttttc ggcacggcgc
     9841 gtccggcgag cgcgccgatg gtctcgagcg cgagcatata gatcggggcg taaggcttgc
     9901 cggcatagac cacgggtccc cccgcctttt catacacggc ggcaagggcg ccggcgcacc
     9961 agacgagctg gtcgcctcgc tcgaccaatt gatcgggatt ggcgcagatc atcggaagct
    10021 ttcgcgcggc aagcgcgctc aagagctcgg aataatcctc gggcgtctcc gtctcgtcat
    10081 cgaacaggcc cgagcagacg atgagctcgg agtcctcgtg acgcccgagc ttgacatcga
    10141 ggccctcgaa gatgggccgg tcgcgcaaag gcccgagatg aaacacggtg gcgccggcgt
    10201 gcttgacgag ctcgtgacgc gtgaggtcgc cggaggtcac ggtcgcgtca taggagtcgt
    10261 cgggaacgcc gagacggcgg agctggtcca tgaccgacgg gctcggccgc ggcgagttcg
    10321 agagcaacac cacgatgccg cctgtcgcgc ggaacgcgcg cgtggcggca accgcgcgcg
    10381 ggaaggcccg atgcccgtta tgcatgacgc cccagatatc gacgagccag ccgcggtaat
    10441 gcgacgccag gcttttgatc gagtcgagga cgggaatgtc gcttgcagcg acggtcatgc
    10501 ctcgtcggtt agagactcgg gcacgacggg tcaagcgcca cgcgtcatgc cgaggcacga
    10561 cctgcctgcg cgaagccggg gcttcgctcc tgcgaaggca gggcgaagca tttccctctc
    10621 cccccgtggg agagggtggc gcgagcgcat gcgagcgcgg ggtgacaggg atcgacgtca
    10681 tgcgcgccgt ggcgcaaccc ctcacccgcc tcagcctcgc ctccgctcgg cttcggcacc
    10741 ctctcccgca aggggagtgg gggaatcctt ggagggcgcg ccgcggcggc cacctcggga
    10801 tgagggcgtg caacg
//
LOCUS       DASUQP010000193        22724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2004534, whole genome shotgun sequence.
ACCESSION   DASUQP010000193 DASUQP010000000
VERSION     DASUQP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 22724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22724
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004534"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1371)
                     /gene="lpxB"
                     /locus_tag="VFY74_08600"
     CDS             complement(160..1371)
                     /gene="lpxB"
                     /locus_tag="VFY74_08600"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX5931789.1"
                     /translation="MDRREPLIFIVSGEASGDNLAGRLMAALKRKTGGRIRFAGVGGP
                     QSESEGLASLFPMSELSVMGLAEVLPHLPRLIGRINETAEAVRRLEPDAVVTVDSPGF
                     SLRLAHHLRGSGIPVIHYVAPQLWAWGPFRARKLKKRVDHIMALLPFEVPFFEKYGIA
                     CTYVGHPAIDAGAEQGDGKAFRLRHGVGPGAPLLCVLPGSRAGEVRRMLPIFGEALLL
                     LKEKHSGLRVVIPAVDSVAEVVRDMTRDWPLPAVLIIDMAERFDAFAAADAAMAKSGT
                     VTLELALAGVPMVVAYRISPVSAFLVRRMGISVEHASLVNLLAGRQVVPERLQEECTP
                     ENLAASVEEVLGSEPVRARQRQAFKDVMKALGDRNPPPSERAASVVLDIVGSRARRLK
                     VEETRGGNSRS"
     gene            complement(1433..1879)
                     /locus_tag="VFY74_08605"
     CDS             complement(1433..1879)
                     /locus_tag="VFY74_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HEX5931790.1"
                     /translation="MVDLSTDPGFFALLTDSYARLVKAPLVPPEADARWLYSEAPFAV
                     LAHGTDHDPKFTYANKAAQDCFEYSLEELVALPSRLSAEPTDRAERQRLLDQVARNGF
                     LAGYSGIRIAKSGRRFVIEDGIIWELIDGAGVRHGQAATFRSWRNV"
     gene            2019..3242
                     /locus_tag="VFY74_08610"
     CDS             2019..3242
                     /locus_tag="VFY74_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEX5931791.1"
                     /translation="MARRAMPQSLQLRDGAAGDAERKRPRGRAEAVPREDDARIVSID
                     VLRGLVMVVMALDHTRDFFGDGSFNPRDVTEPALFLTRWITHLCAPTFVFLAGLSAFL
                     YGRGRSVAETSRFLFTRGLWLILVEFTIVRIAWSFDLALGDTLVGGVIWVIGASMVAL
                     AALVWLPRWAIVAVALAMIAGQNLLDGLRPEVFGGAAPLWHVLHQRGSVHLTEGFRFF
                     VLYPLIPWVGVMAAGYAIGPVMALGPDERNRVLLKLGAAITIGFILLRATNLYGDPAP
                     WTAQETWLATLLSFLNCEKYPPSLFFFMMTLGPALMLLGVFEHARGAFARFLAVFGEV
                     PFFFYVVHIYLIHALAVATGFAVTGALARNPEIGLSLAGIYIVWLIVIVLLYPLCRWF
                     AGLKRGGGGWWWSYL"
     gene            complement(3245..4105)
                     /gene="aguB"
                     /locus_tag="VFY74_08615"
     CDS             complement(3245..4105)
                     /gene="aguB"
                     /locus_tag="VFY74_08615"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEX5931792.1"
                     /translation="MGTRTLAVAAIQASFGPDMAANIAKIERLVREAVRQGAQVILPP
                     ELFQGIYFPTRQDPKWFATAYPASEHPCVLALAKLAKSLEVVIPISFYEKDGPRYFNS
                     VAVADTSGDILGVYRKSHIPDGPGYMEKYYFRPGDTGFKAWNTRAGTLGVGICWDQWY
                     PEAARALVLEGAEILFYPTAIGSEPQDPSLDTHLQWQRAMQGHAVANAVPIVAANRIG
                     VEENDGATQTYYGHSFIADHKGELVARFGRDDEGVLVASFDLDEIARYRAEWGFFRDR
                     RTDLYAKSLR"
     gene            complement(4105..5127)
                     /locus_tag="VFY74_08620"
     CDS             complement(4105..5127)
                     /locus_tag="VFY74_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX5931793.1"
                     /translation="MLEYLSEQAISVPAEWAPQKAIWTAWPADAEEWNGDLASPRRDV
                     AALVRALAPSNQVRLLVAGGEAEASARAALEDAAELIPARYGDIWLRDTGPIFARTAT
                     GPIALRFQTNGWGGKFDLPDDATVGDEIARLARTPARKFDFVLEGGAVEHDGEGTFLA
                     TRETLLNANRNGWTEAQAEAALVEAFGARKIVWLDRGLLNDHTDGHIDVLARLVGPAR
                     VVCQSPYGADDPNADVLAEVARTLEAATDALGRRFELVRMPSPGRVVNELGDALPASH
                     MNFVIANGVVAVPVYGTPSAARAVEALQAVFRDRKVVGLPSLGLLGSGSAGGGSFHCI
                     TREEPA"
     gene            5383..6423
                     /locus_tag="VFY74_08625"
     CDS             5383..6423
                     /locus_tag="VFY74_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5931794.1"
                     /translation="MIFRQLFDSTSSTYTYLLASRHGGEALIIDPVLERVDRYIQLLK
                     ELDLRLVKAIDTHLHADHITGLEALSERTRCITVMGEQTKADVVSIRVGDGDRVGIEG
                     LTLEALYTPGHTDDSYSYLMADRVFTGDTLLIRGTGRTDLQNGDPRAQYESLFGRLLK
                     LPDELLIYPAHDYKGDTVSTIGEEKAFNPRLQVKSVEDYVALMNNLNLPNPKMMDVAV
                     PANMRIGLAQQIVTGKGWAISADEAKNLLGHTEVALIDLRENQERRKNGVIPGSLHVP
                     YPDVEGSLKEDGLLQQLAKSAGKRLVFYCAFGERSAMAVQAAQAAGLATSRHIHGGMQ
                     AWQRAHGPITPS"
     gene            6479..7408
                     /locus_tag="VFY74_08630"
     CDS             6479..7408
                     /locus_tag="VFY74_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX5931795.1"
                     /translation="MFGSRWRLAAVAAVVLSALIALNSGARANIKICQGLEQSYEQIA
                     RGASTVEINSLLFKATDKGCKDLARRLLDAGASLEARDGIGAKPLSHAAAAGEPELVA
                     LFLDKGAPIDARNLDGSTALFKAAENGRLEIVKMLVERGAQVDLPGRSGVTPLSAAAF
                     MGSAPIVEFLIEKGADPKWLDGTGKTAIVYAAGHGFTPVVGLLLDHGIDVNAKYGNDL
                     TALMWSAGYSAEAGVKDILAVMTLLIDRGARLDDQDNRGRTALMIAAELNHAAAADLL
                     LARGADKSLKDKQGRTAADLTSLTALRDKLASR"
     gene            complement(7412..8020)
                     /locus_tag="VFY74_08635"
     CDS             complement(7412..8020)
                     /locus_tag="VFY74_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEX5931796.1"
                     /translation="MALSGLGARGALVVCVLLIGGNASAAEPIAEKAKLCATCHGEQG
                     IPINKQTPVIWGQNEGYLYLQLRDYKSGARKNELMAQVVSTLEKNDFLALAAHFTKLK
                     WPNLQQPSAPPDVAKKAETANASIGCKGCHLEHFQGDGTTARLAGQWHEYLQATMMAF
                     RDGSRGNNPGMSDLMKAASPDDIAALSQYLSGLQIDAFLGNN"
     gene            complement(8125..9696)
                     /locus_tag="VFY74_08640"
     CDS             complement(8125..9696)
                     /locus_tag="VFY74_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5931797.1"
                     /translation="MLSAAAGDDKNFLHTNGNYDQTRYYPAKQINTSNVAKLRPAWIF
                     QTEVKESQETTPIVVDGVMYITTSFNHVYAINAKTGEEYWHYKHKMGPVTTYCCGPNN
                     RGVAIYKDKVYMGTLDAKLVALDSKTGSLVWETQIAEPELGYSETMAPTAVDGKILIG
                     TNGGEYGIRGFVRAYDAETGKMLWNFHTIPENSVGVWATHDATGKDMHRDIAAEKEAL
                     KKLGDPYKTLGGGVWQNPAVDLKTKRIYFVVGNPSPDLDGSIRPGDNLYTDSLVSVDL
                     DTGEYKCHFQYIAHDVWDLDSVSPPILVDVKDKDGKTVPGVIHAGKTGNVYVHKRDDC
                     SLIRFSEAMVDQTGMWSLPTPEGTPMLPGANGGVEWSVMAINPELNMTYAVNLHQPMT
                     YHVESSPYPGGKLWLGGAFKVIPGSEQFGNVTAVDYNTGKIAWQVKTPLPMMGGALTT
                     AGGLVFTGEGDGWFRAYDAKTGKVLWSFFAGAGVNAPPASYSVDGKQYIVVGAGGNVQ
                     IDFKRGNNIIAFTVD"
     gene            10183..11415
                     /locus_tag="VFY74_08645"
     CDS             10183..11415
                     /locus_tag="VFY74_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5931798.1"
                     /translation="MRSIGWILLLLLLLAPLSALAAPQDQEGEAPPSSATATTTIPIG
                     YLKQEVKRPIPLSRLNVEPGDLGIAGASIALKDNNTTGRFTKQQFTLEVERVPAGGDP
                     VQALTKLVESGHHFVLVDAPADVLLRLSDAVKDKDVLLFNISATDVNLRQEDCRANVL
                     HTAPDRAMLADALAQYLVWKKWPRWLVAKGVFPEDLAYLDALRRAAKRFGGTIVEERE
                     YQETPGARRSDTGHQQIQQQMPVFTQGAPDYDVVVVADEREVFGPYLPYRTWDPRPVA
                     GTAGLRAMSWHPAHEQWGATQMQNRFQRFAKRFMLPLDYQAWLAVRAVGEGASRAGSG
                     DFAPVNTHIRGAQFDLAAFKGQKVTFRSWDGQLRQPILVAGPELPVSTSPQEGFLHEH
                     AEVDTLGIDQPESTCKFQ"
     gene            11606..12550
                     /locus_tag="VFY74_08650"
     CDS             11606..12550
                     /locus_tag="VFY74_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HEX5931799.1"
                     /translation="MLMSVMFGAEPADAYTVYISNEKGNSITVLDSATLEVLETIPVG
                     QRPRGIMLTKDGKHLLVCASDDDTVQVLDVATREIVGDLPSGPDPELLNLHPSGSPVY
                     VANEDDNLLTVIDLADKKVLAEVEVGVEPEGVGISPDGKTVVVTSETTNMAHFIDTET
                     FEITDNVLVGSRPRFAEFSSDGSRLWVTSEVAGTVSVIDAKTRKIIKVIGFAVPGVQP
                     EYIQPVGVRVTKDGSKAFVALGPANRVAVIDGKSLEVEDYLLVGKRVWQLAFTPDESL
                     LFATNGNSNDVSVIDVPNLKVVKSIPVGRAPWGVTVSQ"
     gene            complement(12626..12820)
                     /locus_tag="VFY74_08655"
     CDS             complement(12626..12820)
                     /locus_tag="VFY74_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931800.1"
                     /translation="MALPKIVLRGRKIILLSGFKNGARENNKPEALVVDAEDKLRQAA
                     KRHPDAVAFGYVAGEWVAAA"
     gene            complement(13064..14050)
                     /locus_tag="VFY74_08660"
     CDS             complement(13064..14050)
                     /locus_tag="VFY74_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5931801.1"
                     /translation="MGLPYGSKISAEPRIDRKPARNRLVALTGATGFIGRYLLRELPK
                     RGYRLRVLLRRPTTLPEGCAGAVIGDLARPYNMAEALAGVDAIIHTAGLAGTMTGVPE
                     DDYRLFNTEATVGFAKAAQRARIKRFVFLSSIRAQSGPTAEGPLTEIREPQPTDAYGR
                     SKLAAEQGLATIDLDWVALRLALVYGPGVEGNMAKLIKLARSPYPLPLAGLKGEHSLL
                     GLENLVDAVDTVLSADAALRRPLIVADAKPLTIGAMIAAMRRGLGRRPALFYVPQPLL
                     KGTLHVSGRAARFEPLFGSLVADPSELARLKWVPRVDTAAGLAALLRAGTSP"
     gene            14345..15946
                     /locus_tag="VFY74_08665"
     CDS             14345..15946
                     /locus_tag="VFY74_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5931802.1"
                     /translation="MFGAVALAAVAASAPAAAQNIRGDASGPTSAPGFSHPEQYIHLK
                     DVKPAPNLYPVIEHPEQDKAAAAKLAALAGKTGKKPNIMIFLLDDVGWMDPGFNGGGV
                     AVGNATPVMDRLANEGLLLTSAYSTPSCSPTRATIHTGQNPLHHGILRPPMYGEPGGL
                     DGATTMPMLLKPQGYITQGVGKWHMGENQGSLPQNVGYDDYVGFLGVSDMYTEWRDIY
                     FNPEVALSPERFKMLEQDTFNHYEVHCTPADKTKCENGKLIDLAYIKELDRHWMETSL
                     AFLDKMKGSKTPFFLYHATRGCHFDNYPTDDWAGRSMARTVYSDCMVHMDYILGKLVD
                     KLQEIGELENTLIFFTSDNGPECEVPPRGRTPFRGCKGSAWEGGVRVPTFAYWKGMIG
                     PRKSDGLFDLADLLPTALSLAGVPGAKLADLFPKSTYVDGVDQASFLIADDGLSARRS
                     RIYTLNQYYSAIRIDEFKGVATGELETAVVQKGHPGGFSGSIFTDTGGAIVFNLYTNP
                     QEDESIGIRHIPLAVPLGETAGSGT"
     gene            15943..16086
                     /locus_tag="VFY74_08670"
     CDS             15943..16086
                     /locus_tag="VFY74_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931803.1"
                     /translation="MRELFEYPPQFKIGFMSNNPPVYDVGPEVKEGLREFLRQHGFGR
                     LTP"
     gene            16157..17164
                     /locus_tag="VFY74_08675"
     CDS             16157..17164
                     /locus_tag="VFY74_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5931804.1"
                     /translation="MFGRLQARVLRGALIASLVAIASAARAEPSRESCAGATAPSGDS
                     ETAGMVFIPGGSFTMGSDSTHPEERFSHEVRVDGFWIDRHEVTNAEFAEFVKATGYVT
                     LAERGLDPKTHPNMTGDLTAPGSVVFVPPTRLDRGGDITQWWQYVKGASWREPEGPGS
                     SIEGKENYPVVHVAWRGRDLPTEAQWEYAARGGHEIGDDWSEAFDKDGKPIANSWQGI
                     FPVYNTEQDGYGWQAPAGCFAPNAYGLYDMIGNVWEWTADWYVPGHRREAALNPSGPA
                     LLELRVAAGQAPSKVIKGGSYLCSTNYCTRYRATARQPQEADLSAGHLGFRTVLNRPR
                     Q"
     gene            17173..18090
                     /locus_tag="VFY74_08680"
     CDS             17173..18090
                     /locus_tag="VFY74_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5931805.1"
                     /translation="MIRSRRGLAIVAVIAAIALTLAANTLAVDSAMRAAVARSGGTVM
                     ETGIVPANVKVEGEGPPIVLIHGFGAALDWWDDIAPQLAADHRVIRLDLIGHGGTEAP
                     ASGYAIERQAKLVAAVLDKLGVDRVVAIGHSMGGEVATALAEADPKRIERLVLIDSPP
                     KAETTFRLGTRLALMPVIGELLSRFMSDAAIRKMLAQGFAPGFPVPEKFVADVKQLTY
                     TAFRTAHDDSIAYETEKPVVERLAALDPVPPLLVIFGSRDALVAPESAKLYETVPGAK
                     VVILAGPGHSLMVEAPEKTLALIKNFLAQ"
     gene            18125..19138
                     /locus_tag="VFY74_08685"
     CDS             18125..19138
                     /locus_tag="VFY74_08685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5931806.1"
                     /translation="MRNFASRRAALAALIVGIIGVGAEPARTQADPLPSWNDGQAKQS
                     IIDFVTRVTKEGGPDYVEPKDRVATFDNDGTLWIEQPIYTQFAFALDQVKRQANQHPE
                     WSTKEPFKSVIAGDLKAVAAMGEKGMVEIVAATHSGMTTVEFDDTVRDWLKTATHPRF
                     KALYTDLIYQPMRELLDYLRAHGFKTFIVSGGGVEFMRNFTEQTYGIPPEQVIGSSGV
                     TQYQMWDASPVLIKQPKVLFVDDGPGKPEGINHFIGRQPIFAFGNSDGDKEMLEWTAF
                     CRRLCFMGLVHHTDAAREYAYDRDSDVGKLDKALDEALAKGWTVVDMKNDWKTIFPPK
                     AAP"
     gene            19169..19540
                     /locus_tag="VFY74_08690"
     CDS             19169..19540
                     /locus_tag="VFY74_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931807.1"
                     /translation="MLRLLMIVSGTFEALFGLSALIAPNLLIAGLGTETTAAAGLLAR
                     IFGAAALGLGAAALLARDEVNTKGGIAAAFGLALYNVLAACFILWTAAIDHSGAALWG
                     AGLIHAVIGALFVYALAKARG"
     gene            complement(19877..20158)
                     /locus_tag="VFY74_08695"
     CDS             complement(19877..20158)
                     /locus_tag="VFY74_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931808.1"
                     /translation="MRGIFALFFALPVALGASSVAMAAAEDCMVGDSALCLANPNCHW
                     DGEKRGCYPGAPQYQDACAVHTDQGVCDTDTTLGCKWSAGTNKCETKTN"
     gene            20341..21534
                     /locus_tag="VFY74_08700"
     CDS             20341..21534
                     /locus_tag="VFY74_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5931809.1"
                     /translation="MARVVLALLVAGALAGCASAIARHPVPASLEGQAEVVGMGEAPI
                     RFWGDELPPNADAMVKEKWAQVRASRPELLNKGRRPVVNFLALSGGGSDGAFGAGVLA
                     GWTASGKRPEFDLVTGVSTGALTAPFAFLGPKYDEALKKVFTQSSTKDIAIAQPIRGL
                     LGGDSLASNAPLAKVVAFYVNEAFLQEVAAEHDKGRRLLIGTTNLDAERPVIWDMGQI
                     AGSGRPEALDLFRKVLLASAAIPAVFPPGFVNVAANGAVYDEMHVDGGATREVFLVPT
                     QFMAAKTDGGLGINPIRRAYIIRNGHVDPEYKPVKAKTLSIAGRAVSSLIKSQGVGDL
                     YELFVFAKKNGIDYNLAYIPGDFPDTSTQAFDPVYMGKLFDLGFGLAQKGYPWKKVPP
                     RFVGR"
     gene            21641..22666
                     /locus_tag="VFY74_08705"
     CDS             21641..22666
                     /locus_tag="VFY74_08705"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX5931810.1"
                     /translation="MNLAELNRVATAMVAPGKGILAADESGGTIAKRFTAIGVESTET
                     SRRDYRELLFRSTKAMADRISGVILFDETIRQKAEDGSSLVKIIEQAGSIPGIKVDKG
                     TKPLPFSPGETITEGLDGLRERLVEYRDLGAKFCKWRAVIDIGPGIPSYNCISANAHA
                     LARYAALCQEEDLVPIVEPEVLMDGDHDIGRCEEVTEFVLKTVFQELYYARVKLEGMV
                     LKPNMVVPGKKSTKQASDTEIAERTLRVLKACVPAAVPGIAFLSGGQSDVEATARLNV
                     MNAVGGVPWRLSFSYGRALQAAPQKAWSGRAENKAAAQAAFAHRAEMNGLATLGKWSP
                     ELEKRAA"
BASE COUNT         4094 a         7137 c         7382 g         4111 t
ORIGIN      
        1 ctcgactgca tcctcaatgg aacgacgccg ttggtggatg ggcgcgcagg ctgcgaggca
       61 ctccgcgtcg caagcttgat caacgagagc atcgagaagc acctcaagaa ggtgcaaacc
      121 cagccggtga agcccgcggc cggagctgcc ggctcctagt caggagcgcg agttgccccc
      181 gcgtgtctct tcgactttga gcctccgggc gcgcgacccg acaatgtcga gcacgacgct
      241 cgccgcgcgc tcgctcggcg gggggttgcg gtcgccgagc gccttcatca cgtccttgaa
      301 ggcttggcgt tgccttgcgc ggaccggctc cgagccaaga acttcttcga cggacgcggc
      361 caaattctcc ggcgtgcatt cctcctggag gcgttccggc acgacttggc ggcctgcgag
      421 caggttcacg agcgaagcgt gctcgacgct gatccccatg cgccggacga gaaacgccga
      481 gaccgggctg atcctatagg cgaccaccat cggaacgccg gcaagcgcaa gctcgagcgt
      541 gacggtgccc gatttggcca tggcggcatc ggccgcggcg aaggcatcga agcgttcggc
      601 catgtcgatg atgagtaccg cggggagggg ccagtcccgg gtcatgtccc gcaccacctc
      661 ggccacggaa tccacggcgg gaatgacgac gcgaaggccg gaatgtttct ccttcaagag
      721 gagcagcgcc tcgccgaaga tcggaagcat gcggcgcact tcgccggccc ggctgccagg
      781 gaggacgcag agcaggggag cgcctgggcc tacgccgtgc cgcaggcgga acgctttccc
      841 atcgccttgc tccgcgccgg cgtcgatggc gggatggccg acataagtgc aggcgatgcc
      901 gtatttctcg aagaagggca cctcgaaggg cagaagcgcc atgatgtgat cgacgcgctt
      961 cttgagcttg cgcgcgcgga acggtcccca ggcccaaagc tgcggcgcga catagtggat
     1021 tacgggtatg ccggaaccgc ggagatgatg ggcgagcctg agagaaaagc ccggcgagtc
     1081 cacggtcacg acggcatcgg gttcgagccg ccgcaccgcc tcggccgtct cgttgatgcg
     1141 cccgatgagc cgcggcagat gcggcagcac ctcggcaagc cccatcaccg agagctcgct
     1201 catgggaaac aggctcgcaa gcccctcgct ctcgctctgc gggccgccca cgccggcaaa
     1261 gcggatgcgg ccgcccgtct tgcgcttgag cgccgccatc agccgcccgg cgagattgtc
     1321 gcccgaggcc tcgcccgaga cgatgaagat cagcggctca cgcctgtcca tgccaactcc
     1381 aaatgcttgt gccgcgcgcc gctgaacgag aatgaagctg attagcccaa gttcagacat
     1441 tccgccaaga gcggaacgtt gcggcctggc cgtgccgcac gccggcgcca tcgatcaact
     1501 cccatatgat cccatcctcg atcacgaagc gccggccgga cttggcgatg cgaatgccgc
     1561 tgtagcctgc gagaaaaccg ttgcgcgcca cctgatcgag cagcctctgc cgttcggccc
     1621 ggtcggtcgg ctctgccgaa agccgcgacg gcagcgctac gagctcctcc aaggaatatt
     1681 cgaagcagtc ctgtgccgcc ttgttggcgt aggtgaattt cggatcgtga tcggtgccat
     1741 gcgcgaggac ggcgaagggt gcttcgctat agagccagcg cgcgtctgcc tcgggtggga
     1801 cgagcggggc cttgaccagc cgggcgtagc tgtcggtcaa caaggcaaaa aagccgggat
     1861 cggtcgacaa gtctaccatt cggcggtcgg ttcgggtttc catcgtgcga ggatcacggc
     1921 gagcatgtca tcgcgctcgg tgtttgcggc aggattaagc ccgagagaat cgcctttgcg
     1981 ccagaaaatt cgcgcgacat tgcgttagga taagggtcgt ggccagacgc gctatgccgc
     2041 aatcgttgca acttcgagat ggtgccgcgg gagacgcgga acggaaacgt ccccgcggcc
     2101 gagccgaggc ggtgccgcgg gaggatgacg cgcggatcgt gtccatcgat gtcttgcgcg
     2161 gtctcgtaat ggtcgtcatg gcgctcgacc acacgcggga tttcttcggc gacggcagtt
     2221 tcaatccccg cgacgtgacc gagccggcgc tgttcctgac gcgatggatc acgcatcttt
     2281 gcgcgccgac cttcgtcttt ctcgccgggc tctcggcctt cctctatggt cgcgggcgaa
     2341 gcgtcgccga gacgagccgc ttcctcttca cgcgcgggct ttggctcatc ctggtcgaat
     2401 tcaccatcgt acggatcgcc tggagcttcg atctcgcgct cggcgacaca ttggtcggag
     2461 gcgtgatctg ggtgatcggc gcctcgatgg ttgcattggc ggctcttgtc tggctgccgc
     2521 gctgggccat cgtggccgtg gcgcttgcca tgatcgccgg tcaaaacctc ctcgatggtc
     2581 tcaggccgga agttttcggc ggggcggcgc cgctatggca cgtgctgcat cagcggggtt
     2641 ccgttcacct gaccgaaggg ttccgctttt ttgtccttta cccgctgatc ccgtgggtcg
     2701 gagtgatggc cgcgggctac gcgatcggcc cggtgatggc gcttggaccc gacgagcgga
     2761 atcgcgtcct gctcaagctt ggcgccgcga tcacgatcgg gttcatcctg ctgcgcgcca
     2821 ccaacctcta cggcgacccc gcgccatgga cagcgcagga gacttggctc gcgacgctgc
     2881 tctccttcct caattgcgag aaatatccgc cgtcgctgtt ctttttcatg atgacgctgg
     2941 ggccggcgct gatgctgctc ggcgtcttcg agcatgcacg aggggcattt gcgcgcttcc
     3001 ttgcggtatt cggtgaggtg ccgttcttct tctatgtcgt ccacatttat ctaatccatg
     3061 cgctcgcggt cgccaccggc tttgccgtga caggcgcgct cgcacgcaac cccgagatcg
     3121 ggctcagcct cgccggaatc tatatcgtct ggctgatcgt gatcgttctg ctctatcccc
     3181 tttgccgctg gtttgccggg ctcaagcgag gcggcggcgg ctggtggtgg agctatctct
     3241 gagctcacct gagactcttg gcgtagaggt cggtgcggcg gtcgcggaag aagccccatt
     3301 ccgcacggta gcgcgcgatc tcatcgagat cgaagctcgc cacgagcacg ccttcgtcgt
     3361 ctcgtccgaa gcgtgcgacc aattcgcctt tgtgatcggc gatgaaggaa tggccataat
     3421 aggtctgggt tgcgccgtcg ttctcctcga cgccgatgcg gttcgcggcc acgatgggca
     3481 cggcgtttgc gaccgcgtgg ccctgcatgg cgcgctgcca ttggagatgc gtgtcgaggg
     3541 aggggtcctg cggctcggag ccgatggcgg taggatagaa caggatctcg gcgccttcga
     3601 gcacgagcgc gcgggccgct tccgggtacc actgatccca gcagatcccg acgccgagcg
     3661 tgcccgcgcg cgtgttccag gctttgaagc ccgtgtcgcc ggggcggaag tagtatttct
     3721 ccatgtagcc ggggccgtcg ggaatgtggc tcttgcgata gaccccgagg atgtcgccgc
     3781 tcgtgtcggc caccgccacg ctgttgaagt agcgcggccc gtccttctcg tagaacgaga
     3841 tggggatgac gacctcgagc gactttgcga gcttggcgag cgccagaacg caagggtgct
     3901 cgctcgccgg gtaggcggtc gcgaaccatt tcgggtcctg ccgtgtgggg aagtagatgc
     3961 cctggaacag ctcgggcggc aggatcactt gcgcgccctg gcgcacggcc tcgcggacca
     4021 ggcgttcgat cttggcgatg ttggccgcca tgtcggggcc gaaggaggcc tgaatggcgg
     4081 cgacggcgag cgtccgcgtt cccatcacgc cggctcctct cgggtgatgc aatgaaacga
     4141 cccgccgccg gcagaccctg agccgaggag gccgagcgag gggaggccaa cgactttccg
     4201 gtcgcgaaat accgcctgca aggcctcgac cgcgcgcgcg gccgaaggcg ttccgtagac
     4261 aggcacggcg acgacgccgt tcgcgatgac gaagttcatg tgcgaggcgg ggagcgcgtc
     4321 gccaagttca tttacaacgc ggccagggct tggcatgcgg acaagctcga agcgccggcc
     4381 caaggcatcg gttgcggctt cgagcgtgcg cgcgacctcc gccaagacgt cggcattggg
     4441 atcgtcggcg ccgtaggggg attgacacac gacgcgcgcc ggtcccacga gacgcgcaag
     4501 cacatcgata tgcccgtccg tgtggtcgtt gaggaggccc cggtcgagcc agacaatttt
     4561 tcgcgcgccg aaagcttcga cgagcgccgc ttcggcttgc gcctccgtcc agccattgcg
     4621 attggcgttg agcagggtct cgcgcgtggc gaggaacgtg ccttcgccgt catgctcgac
     4681 ggcgccgcct tccagcacga agtcgaattt gcgggccggt gtgcgcgcaa gccgtgcgat
     4741 ctcgtcgccg accgtagcgt cgtcgggaag atcgaacttg ccgccccagc cattggtctg
     4801 gaacctgagg gcgatggggc cggttgccgt ccgcgcgaaa atcgggccgg tatcgcgcag
     4861 ccagatatcg ccgtagcggg cgggaataag ctccgccgcg tcctcgagcg cggcccgtgc
     4921 cgaggcttcg gcttctccgc ccgccaccag gaggcgcact tgatttgagg gggcgagcgc
     4981 gcgcacgagc gccgccacgt cgcggcgagg gctcgcgagg tcgccgttcc actcctcggc
     5041 atcggcaggc caggcggtcc agatcgcctt ctgcggcgcc cattcggccg gaacggaaat
     5101 cgcctgctct gatagatact cgagcatatg gtctacctat gggcccgagt acgacttaac
     5161 gccgtaaacc gccgggtctt tctctctcaa gggcgtaaag ggggcggtgt caacccgctt
     5221 gaaggcacgt cgcgccgtca gcgccttggc ttgacggcgg caacgcccgg gctcgccaga
     5281 aactccgtca tgcccgctct actgctatag ttggggcagg ccttgcggtc taccgcacac
     5341 gacgcttttc ttgtgcgggg ctcacgcaat cagagactcg tcatgatctt ccggcagctg
     5401 ttcgactcga cctccagcac ctacacctat cttctcgcga gccgccatgg cggagaggcg
     5461 ctcatcatcg accccgtgct cgaacgggtc gaccgttaca tccagctcct gaaggagctc
     5521 gatctcaggc tggtgaaggc gatcgatacc catctccacg ccgaccacat caccggactt
     5581 gaagcgctga gtgagcgcac gcgctgcatc acggtgatgg gcgaacagac caaggccgac
     5641 gtcgtttcca taagggtggg cgacggcgac agggtcggca tcgaaggact caccctcgag
     5701 gcgctgtata cgccggggca taccgacgat tcctacagct acctcatggc cgaccgcgtg
     5761 ttcaccggcg acacgctgct gatcagggga accggccgca cagacctgca aaacggcgat
     5821 ccgcgggcgc aatacgagtc gctgttcgga cgcctgctca agcttcccga cgagttgctg
     5881 atctaccccg cgcacgatta caaaggcgac accgtctcga ccatcggcga ggagaaggct
     5941 tttaacccgc gtcttcaggt gaagtccgtc gaggactatg tcgcgctgat gaacaatctc
     6001 aacctgccca accccaaaat gatggatgtc gcggtgccag ccaatatgcg catcgggctt
     6061 gcgcaacaga tcgttacggg caaaggctgg gcgatcagcg ccgatgaggc caagaatctc
     6121 ctcggtcaca ctgaggtcgc cttgatcgac ctcagggaga accaggagcg gcgcaagaac
     6181 ggggtcatcc cgggatcgct gcacgttccc tatcccgatg tcgaaggaag cctgaaggaa
     6241 gatggcctct tgcagcagct ggcaaagtcg gccgggaaac gcctcgtgtt ctattgcgcc
     6301 ttcggcgagc ggtccgccat ggcggtgcag gccgcgcagg cggccggtct tgcaacctca
     6361 cgtcatatcc atggcgggat gcaggcctgg cagcgcgcgc acgggcctat caccccttcc
     6421 tgatgagtta tgctagcaat cggacccgat gggccgtgag gtgagaagga gtgcaggaat
     6481 gttcgggagt cggtggcgcc tcgccgcggt cgcggctgtc gtcttgtcgg cgctgatagc
     6541 gttgaattcg ggggcgcgcg ccaacatcaa gatctgccag ggtctcgagc aaagctacga
     6601 gcagatcgcg cgcggggcga gcacggtcga gatcaatagc ctgctgttca aggcgaccga
     6661 caagggctgc aaggacctcg cgcgccgcct cctcgacgcg ggcgcgtcgc tcgaagcgcg
     6721 ggatggaatc ggcgcgaagc cgctgtcgca cgcggctgcg gctggcgagc ccgaacttgt
     6781 cgcgctgttt ctcgataagg gcgcaccgat cgacgcgcga aacctcgatg gctcgacggc
     6841 actgttcaaa gccgccgaga acgggcggct cgagatcgtg aaaatgttgg tggagcgcgg
     6901 ggctcaggtc gatttgcccg gccgcagcgg ggtgacgccg ctctcggccg ccgccttcat
     6961 gggcagcgcg cccatcgtgg agttcctgat cgagaaaggc gccgatccga agtggctcga
     7021 cggcaccgga aaaacagcca tcgtctatgc cgcgggccac ggctttaccc cggtcgtggg
     7081 tctccttctc gatcatggca ttgacgtcaa cgccaagtac ggaaacgacc tcaccgcgct
     7141 gatgtggtcg gccgggtatt ccgccgaagc cggcgtgaag gacattcttg cggtcatgac
     7201 cctcctcatc gatcgcggcg cgcgtctcga cgaccaggac aatcgcgggc gaaccgcgct
     7261 gatgatcgcc gcggagctca atcatgcggc ggcggcggat ttgctgctcg cgcgcggggc
     7321 ggacaaaagt ctcaaggaca agcagggcag gaccgcggcc gacctgacgt cgctcacagc
     7381 gctgcgcgat aagctcgcgt cgcgctaggc gctagttatt gccgagaaaa gcgtcgatct
     7441 gcaggccgga gagatattgc gacagggcgg cgatgtcgtc gggcgacgcc gccttcatca
     7501 ggtcggacat gccgggattg ttgccgcgcg agccgtcgcg gaacgccatc atcgtggctt
     7561 gaagatattc gtgccactgg ccggccaaac gcgccgtggt gccgtcaccc tggaagtgtt
     7621 cgaggtggca gcccttgcag ccgatcgacg cgttggccgt ctcggccttc ttggcgacat
     7681 cgggcggcgc cgacggttgt tgcaggttcg gccatttcag cttggtgaaa tgggccgcga
     7741 gcgccaagaa gtcgttcttc tcgagcgtgg agacgacctg cgccatcagc tcgttcttgc
     7801 gcgccccgct cttgtaatcg cggagctgca agtagagata gccctcgttc tggccccaga
     7861 tgacgggtgt ctgcttgttg atggggatgc cctgctcgcc atggcacgtg gcgcaaagct
     7921 tcgccttctc cgcgatgggc tcggccgcgg aagcgttgcc gccgatcaag aggacacaaa
     7981 caacaagcgc gccgcgcgcc cccaaaccgc tcagtgccat tctctcagac tccctaagtc
     8041 aagtcgtagg ccggaactcg atcatgaaaa ggacggggca cgaatgcccc gtcctctcgc
     8101 atcgtaatcg cgtcggcgaa acgatcaatc cacggtgaag gcgatgatgt tgttgccgcg
     8161 cttgaagtca atctgcacgt taccacccgc accgacgacg atgtattgct tcccgtcgac
     8221 ggagtaggag gccggcggcg cattgacgcc ggcgccggcg aagaacgacc acaacacctt
     8281 gccggtcttg gcgtcatagg ccctgaacca gccgtcgcct tcaccggtga acacgaggcc
     8341 gccggcggtc gtgagcgcgc cgcccatcat gggcaacggc gtcttcacct gccaggcgat
     8401 cttgccggtg ttgtaatcga cggcggtgac gttgccgaac tgctcggagc ccgggatcac
     8461 cttgaaggcg ccgccgagcc agagcttgcc gcccggatag ggcgaactct cgacgtgata
     8521 ggtcatcggc tgatgcagat tgaccgcata cgtcatgttg agctcggggt tgatcgccat
     8581 gaccgaccac tccacgccac cgttggcgcc gggcagcatg ggcgttcctt cgggcgtcgg
     8641 cagcgaccac atgccggttt gatccaccat cgcttccgag aagcggatca ggctgcaatc
     8701 gtcgcgcttg tggacgtaga cgttgccggt cttgcccgcg tgaatgacgc ccggaacggt
     8761 cttgccgtcc ttgtccttca cgtcgaccag gatcggcggg ctgacggagt cgaggtccca
     8821 tacgtcatgg gcgatgtact ggaagtggca cttgtactcg ccggtgtcga gatcgacgga
     8881 gacgagcgag tcggtgtaga gattgtcgcc gggacggatc gagccgtcga ggtccggtga
     8941 cgggttgccg accacgaagt agatgcgctt cgtcttgagg tcgaccgccg gattctgcca
     9001 cacgccgccg ccgagcgtct tgtaggggtc gcccagcttc ttgagcgcct ctttctcggc
     9061 cgcgatgtcg cggtgcatgt ccttgccggt ggcgtcatgg gtcgcccaca cgccgaccga
     9121 gttctcgggg atggtgtgga agttccacaa catcttgccg gtctcggcgt cataggcgcg
     9181 cacgaagccg cggatgccgt actcgccgcc gttggtgccg atcaggattt tgccgtcgac
     9241 ggccgtcggc gccatggtct cgctatagcc aagctcgggc tccgcaatct gggtctccca
     9301 aaccaggctg ccggtcttgg aatcgagcgc gacgagcttc gcatcgagcg tgcccatgta
     9361 gaccttgtct ttgtagatgg cgacgccgcg attgttcgga ccgcagcaat aagtcgtgac
     9421 cggacccatc ttgtgcttgt agtgccagta ctcctcgccg gtcttggcgt tgatggcata
     9481 gacgtggttg aaggacgtgg tgatgtacat caccccatcg acgacgatcg gcgtcgtctc
     9541 ctgcgactcc ttcacctcgg tctggaagat ccaggccgga cgaagcttgg cgacgttgga
     9601 cgtgttgatc tgcttggccg gatagtaacg ggtctgatcg taattgccgt ttgtgtgcag
     9661 gaagttctta tcgtcgccgg cagcggcgct gagcatctcc tgcgtcaccg acggggcggg
     9721 cgcgtccttg gctttctttg tcgcggcggc ctcggtttca gctccaccac tgaaggccgg
     9781 gctaaacgcg gcaccggcca gacttacgat tagcgcacca gcaaacgtgg cggctgtctt
     9841 acaacacccc atgatttcct ccttagcgtg gtcccgattc ggccggctgg gtaccgaccg
     9901 ctccagacgg gttctcacgg tgggggagac tgcgcgcggc cttttaattt gtcaatgaaa
     9961 cttgccaata ggtgcggcat gatcggcagc gagatttacc actcttagcc gtttttgctg
    10021 ctgtcttggt cgacgacaag cgggtaagtt tggccgcagc cgggggttcc ctggcccgat
    10081 tttgtgacgc gcgtgcaggc tgcatacaga atgcaatccc gacaggtttg tcgatcgcgg
    10141 gggtcattgg actcccgccg cgcaatcgtg ggcccggatt ggttgagatc gatcggctgg
    10201 atccttttgc tcctgctgct gcttgcgccg ctttccgctc tcgccgcccc gcaagatcaa
    10261 gagggggagg caccgccgtc atccgcgact gcgaccacga ccataccgat cggctatctc
    10321 aagcaggagg tgaagcgacc tattcccctg tcgcgtctca acgtggagcc aggggatctc
    10381 ggcatcgcag gcgcttcgat cgcgctcaag gacaacaaca ccaccggccg cttcaccaag
    10441 cagcaattca cccttgaggt cgaacgcgtg ccggcgggcg gcgatcccgt gcaagcactc
    10501 accaagctcg tggagagcgg tcaccatttt gttctggtgg atgccccggc tgacgtgctt
    10561 ttgcgactct cggacgcggt caaggacaag gacgtgctcc tgttcaatat cagcgcgacc
    10621 gacgtcaatc tgcggcagga ggactgccga gccaatgtgc tgcacacggc gccggaccgc
    10681 gccatgctgg ccgacgctct cgcccagtat ctggtgtgga agaaatggcc gcgctggctc
    10741 gtggcgaagg gcgtctttcc cgaagacctt gcctatctcg atgcgcttcg ccgggcggcg
    10801 aagcgcttcg gcggaacgat cgtcgaggag cgcgaatatc aggagacgcc gggcgcgcgc
    10861 cgctccgaca cgggtcacca gcagatccag cagcagatgc cggtcttcac gcaaggcgcg
    10921 ccggactacg atgtcgttgt cgtggccgac gagagagaag tcttcggccc ctatctgcca
    10981 taccgcacct gggacccgcg accggtcgca ggcaccgcag gtctaagagc catgagctgg
    11041 catcccgcgc acgaacaatg gggcgcgacg cagatgcaga atcgcttcca acgtttcgcc
    11101 aagcgcttca tgctcccgct cgattatcag gcctggctgg cggtgcgcgc cgtgggcgag
    11161 ggggcctcgc gcgcgggctc cggcgatttc gcgccggtca acacgcatat ccggggcgcg
    11221 cagttcgacc ttgcggcgtt caagggacag aaagtcacct ttcgctcatg ggatgggcag
    11281 ctgcggcaac cgatcctcgt cgcgggtccc gaattgcccg tgtcgacgtc gccgcaagag
    11341 ggcttcctgc acgagcacgc cgaagtcgac acgctcggca tcgatcagcc ggagagcaca
    11401 tgcaaattcc agtgagtgaa cctcgtcatc ctgaggtgcg agcgaagcga gcctcgaagg
    11461 atgacattct gtgttgaggc aatccttcga gggccgcctt cggcggcctc ctcaggatga
    11521 cgctcagaag cgagtcaggt cgaggagcag acgcgtgagg atgacagtaa tgaccatagg
    11581 gcgagccgcg tgggcggcgg cagccatgtt gatgagtgtc atgttcggag ccgagccggc
    11641 cgacgcctac acggtctaca tctcgaacga gaagggcaat tcgatcacgg tgctcgacag
    11701 tgccacgctc gaggtgctcg agaccattcc ggtcggccag cgcccgcgcg gcatcatgct
    11761 gaccaaggac ggcaagcatc ttctcgtctg cgcgagcgac gacgacacgg tgcaggtgct
    11821 tgacgtcgcc acccgcgaga tcgtcggcga cttgccgtcg ggacccgatc cggagttgtt
    11881 gaacctgcat ccttcgggca gcccggtcta cgtcgccaac gaggacgaca atctgctcac
    11941 cgtgatcgac ctcgccgaca agaaggtctt ggccgaggtc gaggtcggtg tcgagccaga
    12001 aggtgtgggc atcagtcccg acggaaagac cgtcgtcgtc acctccgaaa ccaccaacat
    12061 ggcccacttc atcgacaccg aaaccttcga gatcaccgac aatgttctgg tcggttcgcg
    12121 gccgcgcttc gccgagttct cctcggacgg gagccggctt tgggtgacct cggaggtggc
    12181 cggcaccgtc tccgtcattg acgccaagac gcgcaaaatc atcaaggtca tcggtttcgc
    12241 cgtcccgggc gtgcagcccg aatatattca gcctgtcggc gtccgcgtca ccaaggacgg
    12301 cagcaaagcg ttcgtggcgc tcggaccggc caatcgcgtg gcagtcatcg acggcaagag
    12361 cctcgaagtc gaagactatc ttcttgtcgg aaaaagggtg tggcaacttg ccttcacgcc
    12421 cgacgagtcc ttgctgtttg cgaccaacgg caacagcaac gacgtctcgg tgatcgacgt
    12481 gccaaacttg aaagtggtga aatcgattcc cgtcggccgg gcaccctggg gcgtgaccgt
    12541 ctcacaatga gtgaacggtc agcgcccgta cccgtggccc ttggagcttt gcctacttag
    12601 ctttcccttg cttttagcgc cgagcctatg ccgcggcgac ccattccccg gcgacgtaac
    12661 cgaaagccac ggcatcggga tgccgcttcg cggcctggcg caacttgtcc tcggcgtcca
    12721 caacgagagc ttcaggcttg ttgttctccc tggccccgtt cttgaagccg ctgaggagaa
    12781 tgatcttcct gccacgcagg acaatcttcg ggagtgccat ccgaacctcc tcgacttgcc
    12841 tgcaagatgt gctctgagtt ctggtcacgg gggctcagat gcacgcccta aaggcaagtg
    12901 cctcgttcct aacggcgacc cccacgtcag ccatgatcac catagcaaaa aattgcgacg
    12961 aacgtaagac aacaggctcg cacttgcgtt aagggctttg cagtgcacaa cgcgtccgcg
    13021 tttgcgctgc atgcatagcg ttagcgtccg ctgcgatgca gcatcaaggt gacgtgccgg
    13081 cgcggagcaa cgcggcaagc cccgccgcgg tgtcgacacg cggcacccat ttgagccgcg
    13141 cgagttccga cggatcggcg acaagagagc cgaacagcgg ctcaaatctc gcggcccggc
    13201 ccgacacgtg aagcgtgcct ttgagcaggg gctgcggcac gtagaagagg gccggtcgcc
    13261 tgccgagacc gcgccgcatc gcagcaatca tcgcgccgat ggtgagcggc ttggcatcgg
    13321 cgacgatgag cgggcgccgc aacgccgcgt ccgccgagag caccgtatcg acggcatcca
    13381 caagattctc gagaccgagc aaagagtgtt cgcccttgag cccggcgagc ggcagagggt
    13441 agggcgagcg ggcgagcttg atcagcttcg ccatgttccc ttcgacaccg ggtccgtaca
    13501 cgagtgcaag cctgagcgcc acccagtcga ggtcgatggt ggcgaggccc tgttcggcgg
    13561 cgagtttcga gcgaccgtag gcgtcggtcg gttgcggctc gcgaatttcg gtgagcgggc
    13621 cttcggcggt cgggccggat tgcgctctga tcgacgatag aaacacgaag cgcttgatcc
    13681 ttgcccgctg cgccgccttg gcgaagccga cagtcgcctc ggtgttgaac aggcgataat
    13741 cgtcttccgg cacgccggtc atggtgccgg caagaccggc ggtgtggatg atggcgtcga
    13801 cgccggcgag cgcctcggcc atgttgtagg gccgggccag atcgccgatg accgcgccgg
    13861 cgcagccttc gggcaaggtc gtggggcgcc ggagcaagac gcgcaggcgg tagccgcgct
    13921 tcggcagctc tctcagcaga tagcgtccga tgaagcctgt ggcgccggtc aacgcgacaa
    13981 ggcgattgcg cgccggtttc cggtcaatcc gcggctctgc gctgattttt gatccgtagg
    14041 gaaggcccat caaatctcct tccgttcagc agcagttgac gcgcatattt tggccggagc
    14101 aagcccgcga taagcccagg ccgccccgcc cgtcagttga ccctcgccgt ttgccatcag
    14161 ttaagcacga tcggggtcgc gcttgccaaa cggccggggc tggcatcatg gaaatgcaat
    14221 ccagtgcagg ctgaagccgg cacgacgcac ggccagtcga tggccgcgcg aaacagggag
    14281 gaaaaagatg cggccgtttg atcgggttca cgcttcggaa tgggcttcag gccaattcgc
    14341 ttggatgttc ggcgctgtcg cgttggcagc ggtagcggcg tctgcgcctg cggctgcgca
    14401 gaacatccgc ggggacgcga gcggtcccac ctctgcgccc ggcttcagcc atcccgagca
    14461 atatatccac ctcaaggacg tcaagcccgc ccccaatctc tatccggtga tcgagcatcc
    14521 cgagcaggac aaagcggcgg cggcgaagct tgccgcgctc gcggggaaga ccggcaagaa
    14581 gcccaacatc atgatcttcc tgctcgacga cgttggctgg atggaccccg gcttcaatgg
    14641 cggcggggtg gcggtcggaa acgccacgcc ggtgatggac aggcttgcca atgaaggcct
    14701 cctgctcacc tcggcctact cgacgccgtc ctgctcgccc acgcgcgcca ctatccatac
    14761 cggacagaac ccgctccatc acggcatcct gcgtccgccg atgtatggcg agcccggcgg
    14821 tctggacggt gccacgacga tgccgatgct gctgaagccc caaggctaca tcacgcaagg
    14881 cgtcggcaag tggcatatgg gcgagaacca aggctctctg ccgcagaatg tcggctacga
    14941 cgactatgtc gggttcctcg gcgtctcgga catgtacacg gaatggcgcg acatctattt
    15001 caatcccgag gtcgcgctat cgcccgaacg cttcaagatg ctggagcagg acaccttcaa
    15061 ccactacgag gtgcactgca cgccggccga caagaccaag tgcgagaacg gcaagctgat
    15121 cgatctcgcc tatatcaagg agctcgaccg gcactggatg gagacgagcc tcgccttcct
    15181 cgacaagatg aaggggagca agacgccgtt cttcctctac cacgcgacgc gcggctgcca
    15241 tttcgacaac tatcccaccg atgattgggc gggaaggtcg atggcgcgca ccgtgtacag
    15301 cgactgcatg gttcacatgg actacattct cggcaagctc gtcgacaaac tccaggagat
    15361 cggcgagctt gagaacacgc tgatcttctt cacctccgac aacgggcccg aatgcgaggt
    15421 cccgccgcgc gggcgcaccc cgttccgcgg ctgcaaggga tcggcctggg aaggcggcgt
    15481 gcgcgttccg accttcgcct actggaaggg gatgatcggc ccgcgcaagt ccgatggctt
    15541 gttcgatctc gccgatttgc tgccgacggc gctgtcgctc gccggcgttc cgggcgccaa
    15601 gctcgccgat ctgttcccca agtcgaccta tgtcgacggc gtcgaccagg cctcgttcct
    15661 catcgccgat gacggcttgt cggcgcggcg cagccgcatc tacaccttga accagtacta
    15721 ctccgccatt cgcatcgacg agttcaaggg tgtcgccacc ggcgagctcg agacggcggt
    15781 tgtgcagaaa ggacatccgg gcggattcag cggctcgatc ttcactgata cgggcggggc
    15841 catcgtgttc aacctctaca cgaacccgca ggaggatgag agcatcggta tccgtcatat
    15901 tccgcttgcc gttccgcttg gggaaacggc aggctctggt acttgaggga gctgttcgaa
    15961 tatccgccgc agttcaagat cggcttcatg tcgaacaatc cgccggtcta tgatgtcggt
    16021 cccgaggtca aggaggggct gagggagttc ctaaggcagc acggatttgg cagattgaca
    16081 ccgtaactgc tcatgcgtcg gtgatggtgc ttcgggcaat cgcgccaggc ttgatcgagt
    16141 gacccaaagg atgcggatgt ttggccgatt gcaggcaagg gtgcttcgcg gggcgctgat
    16201 cgcgagcctc gttgcaatcg catcagcggc gcgggcggaa ccgtcgcgcg agagctgcgc
    16261 cggcgctacc gccccctcgg gcgatagcga gaccgcgggg atggttttca ttcccggcgg
    16321 cagcttcacc atgggatcgg atagcaccca ccccgaagag cggttcagcc atgaagtgcg
    16381 tgtcgacggc ttctggatcg atcggcatga ggtgacgaat gccgagttcg ccgaattcgt
    16441 caaggcgacc ggttacgtga cgctcgccga gcgggggctc gatccgaaga cgcatccgaa
    16501 tatgaccggg gacctgacgg ccccgggctc ggtcgtcttc gttccgccga cccgactcga
    16561 tcgcggcggc gacatcaccc agtggtggca atatgtgaag ggcgcaagct ggcgcgagcc
    16621 ggagggtccc ggttcatcga tcgagggcaa ggagaattac ccggtggtgc atgtcgcctg
    16681 gcgcggccgg gatcttccga ccgaggcgca atgggaatat gccgcgcgcg gcggacacga
    16741 aatcggcgac gattggagcg aggccttcga caaggacggc aagccgatcg ccaatagctg
    16801 gcaaggcatc ttcccggtct acaacaccga gcaagacggc tatggctggc aagcgcccgc
    16861 cggctgcttc gcgcccaatg cttacgggct ctacgacatg atcggcaatg tttgggaatg
    16921 gaccgcggac tggtatgtgc cgggccatcg gcgcgaggcc gcgctcaatc cgagcggtcc
    16981 cgccctgctt gagctccgtg tcgccgcggg gcaggcgcca agtaaagtca tcaaaggcgg
    17041 ctcctatttg tgctcgacca attattgcac gcgctatcgc gcgaccgcgc ggcaaccgca
    17101 ggaagctgat ctcagcgccg gccatctcgg ctttcgcacg gtgttgaacc ggccccggca
    17161 atagcgccgc ccatgatccg gtcccggcgc ggcctcgcca ttgtggcggt tatcgccgcg
    17221 attgccctca cgctcgccgc caatacgctc gccgtcgatt cggcgatgcg cgccgctgtt
    17281 gcgcgaagcg gcgggaccgt catggagacc ggaatcgtgc cggcgaatgt gaaggtcgag
    17341 ggggaggggc cgccgatcgt tctcattcac gggttcggtg cggcgctcga ttggtgggac
    17401 gacatcgcgc ctcagctcgc ggccgatcac cgcgtcattc gcctcgatct gatcggccac
    17461 ggcgggaccg aggcgccggc gtcggggtac gcgatcgagc ggcaggcgaa gctcgtggcg
    17521 gcggttctcg ataagcttgg cgttgatcgc gtcgtcgcga tcgggcactc catgggtggc
    17581 gaggtggcaa ctgctctggc cgaagccgat ccgaaaagga tcgagcgtct cgtgctgatc
    17641 gattccccgc cgaaggccga gacgacgttc aggctcggaa cccggctcgc gctgatgccg
    17701 gtcattggcg agttgctgtc gcgcttcatg tcggatgcgg caatccggaa gatgctggcg
    17761 caaggcttcg cgcccggctt tccggtgccg gagaaattcg tcgccgacgt caagcagctc
    17821 acctataccg cgttccgcac ggcacatgac gacagcatcg cctatgagac ggagaagccc
    17881 gtcgtggagc gtcttgccgc cttggacccg gtgccgccgc tgctcgtcat cttcggctcg
    17941 cgcgacgcgc tggtggcgcc ggaaagcgcc aagctctatg aaaccgtgcc gggcgcaaag
    18001 gtcgtgatcc tcgccggccc gggtcattcc ctgatggtcg aggcgccgga gaaaacactc
    18061 gcgctgatca agaattttct cgcccaatga ttagactgca cacggatgtt tcgaggtgag
    18121 tgcgatgcgc aacttcgcca gccgccgcgc cgctctcgcg gcgctaatcg tcgggatcat
    18181 cggcgtcggg gcggagcccg cgcggacgca agctgatccg ctgccatcct ggaatgacgg
    18241 tcaggccaaa cagtcgatca tcgatttcgt gacgcgggtg acgaaagagg gtggccccga
    18301 ctatgtcgag ccaaaggatc gcgtcgccac cttcgacaat gacggcacgc tgtggatcga
    18361 gcagccgatc tacacgcaat ttgctttcgc gctcgaccag gtgaagaggc aagcgaatca
    18421 gcacccagaa tggagcacca aggagccgtt caaatcggtg atcgcaggcg acttgaaggc
    18481 ggtcgccgcc atgggcgaga agggcatggt cgagatcgtg gccgccacgc attcgggcat
    18541 gaccacggtc gagttcgacg ataccgtcag agactggctc aagacggcga cgcatccgcg
    18601 cttcaaggca ctttacaccg atctcatcta ccagccgatg cgcgagctcc tcgactacct
    18661 ccgtgcccat ggcttcaaga cgttcatcgt ctccggcggc ggcgtggagt tcatgcggaa
    18721 tttcaccgaa caaacctacg gcatcccgcc cgagcaggtg atcggctcct cgggcgtgac
    18781 ccagtatcag atgtgggatg caagccccgt cctgatcaaa cagcccaaag tgctgttcgt
    18841 cgatgacggg ccgggcaagc ccgaaggcat caatcatttc atcgggcggc agccgatctt
    18901 cgccttcggc aattccgacg gcgacaagga gatgctggaa tggacggcgt tctgccgacg
    18961 gctatgcttc atgggcctcg tccaccacac cgatgccgcg cgcgaatacg cctatgaccg
    19021 cgactccgac gtgggcaagc tcgacaaggc gctcgacgag gcgcttgcca agggttggac
    19081 cgtggtcgac atgaagaacg actggaagac gatcttcccg ccgaaagccg cgccctagaa
    19141 cgcggccgcg cgacccgaag gtgtgaacat gcttcggctg ctcatgatcg tgtcaggcac
    19201 gttcgaggcg ctgttcggtc tgtcggcgct gattgcgcca aacttgctga ttgccgggct
    19261 cggaacggaa acgacggccg ctgcggggct tctcgcccgc atcttcggcg ccgcggcgtt
    19321 gggacttggt gccgcggcac tccttgcgcg cgacgaagtg aacaccaaag gcggtattgc
    19381 cgcggccttc gggcttgcgc tctacaacgt gctcgccgct tgcttcatcc tttggactgc
    19441 cgctattgac cacagcggcg cggcgctgtg gggcgctggc ctgatccatg ccgtgatcgg
    19501 cgcgctcttc gtctacgcgc tcgcgaaagc caggggatga agtcgctcac aattcgggat
    19561 accggcgcaa gccggtatcc agagccacaa acgccggtgc atgccgcttc tggattccgg
    19621 cgttcgccgg aatgacggat gagtctccgc cggacattgc tgtcgcctta ttccgctctc
    19681 cccttgggga gagcacgcgc gcgctttcga tgcgctaccg cttagctgct tgagcgcaag
    19741 gcgcgcgcgg tgaggggtca ggtgcgaagc gccgtggccg aaccccctca cccttcccgc
    19801 caggcgactt gcgcagagct gcgctcgcgc aagatcgcga cggaaagccc tctcccacga
    19861 agggagaggg gaaaactcag tttgtcttgg tctcgcactt gttggtgccg gcggaccact
    19921 tgcagccaag cgtggtgtcc gtgtcacaga ccccctggtc ggtatggacg gcgcaggcat
    19981 cttggtattg gggggcgccc ggatagcatc cgcgtttctc gccgtcccag tggcaattgg
    20041 gattggcgag gcaaagggct gaatcgccga ccatgcaatc ctcggctgca gccattgcga
    20101 cactgctcgc gccgagcgcg acgggtaggg caaaaaagag agcaaagata ccgcgcatgg
    20161 ggcctccttc gatggacgga cgttataccc tgtctcgttt ggggatggac actcccgatc
    20221 tgcgtcgcgg ccgcggtcga taggctgcct acgccataat tttgcgctaa tgtgctgccc
    20281 tacaagaaaa agtcctataa gggcaaggcg ccccgcaagg gacgttgcaa ggggggtgac
    20341 gtggcgcggg tcgtgttggc gttgctcgtt gccggagcgc tcgcgggctg cgcgtctgcc
    20401 atcgcccgcc atccagtccc cgcttccctt gaaggccagg cagaggtggt gggcatggga
    20461 gaggcgccca tccgcttctg gggcgatgag cttccgccca atgccgatgc catggtcaag
    20521 gagaagtggg cgcaagtccg cgcgagccgg ccagagctcc tcaacaaagg ccggcgcccc
    20581 gtcgtcaatt ttcttgcctt gtcgggcggc ggctcggacg gcgctttcgg cgcaggtgtg
    20641 ctcgccggct ggaccgcgtc tgggaagcgg cccgaattcg atctcgtcac cggcgtctcg
    20701 accggcgcgc tcaccgcacc gttcgccttt ctcggaccca aatacgacga ggcgctgaag
    20761 aaggttttca cccaatcgag caccaaggac atcgccatcg cccagccgat caggggcctg
    20821 ctcggcggcg attcgctcgc cagcaatgcg ccgctggcga aagtcgttgc cttctacgtg
    20881 aatgaagctt tccttcagga ggtggcggcg gagcatgaca agggccgccg gcttctgatc
    20941 ggcaccacca atctcgatgc cgagcgcccc gtgatctggg acatgggcca gatcgccgga
    21001 agtggccgcc cggaagcgct cgatctcttc cgcaaagtgc tgctcgcctc ggcggctatt
    21061 ccggcggtgt tcccgcccgg cttcgtcaac gtggccgcca atggagccgt ctatgacgag
    21121 atgcatgtgg atggcggagc gacacgcgag gtgttcctcg tgccgacgca attcatggct
    21181 gcgaagacgg atggcggcct cggcataaac ccgatccggc gcgcttacat catccgcaac
    21241 ggccacgtcg atccggaata caagccggtg aaggccaaga ccttgtcgat tgccggccgg
    21301 gccgtctcga gcctgatcaa gagccaaggc gtcggcgatc tctacgagct gttcgtgttc
    21361 gcgaagaaga acggcatcga ttacaacctc gcctacatcc ccggcgactt ccccgacacg
    21421 agcacgcagg ctttcgaccc tgtctacatg ggcaagctgt tcgatctcgg ctttggactc
    21481 gcgcaaaagg gctacccgtg gaagaaggtc ccgccgcgat tcgtcggtcg gtagagcatg
    21541 cgtcctggcc ttgagaaacc ctcccgctga ccgttgagct tcggctttgg ctggcctaca
    21601 caaagggcgg gagaaatcct tctcagcgat ggagaggacg atgaatctcg ctgaattgaa
    21661 cagagtggcg acggccatgg tggcgcccgg caaaggcatt ctcgccgccg acgagtccgg
    21721 tggaacgatc gcgaagcgct tcaccgccat cggcgtcgaa tccaccgaga ccagccgccg
    21781 cgactaccgt gagctcctgt tccgctcgac caaggccatg gcagaccgta tctctggcgt
    21841 cattctgttc gacgagacaa tccggcagaa ggcggaagac ggaagctcgc tcgtcaagat
    21901 aatcgagcag gccggctcga ttcccggcat caaggtcgac aagggaacga agccgcttcc
    21961 cttctccccc ggcgagacca tcaccgaagg cctcgacggc ttgcgcgagc gattggtcga
    22021 gtatcgggac cttggcgcga aattctgcaa gtggcgcgcg gtcatcgata tcggacctgg
    22081 catccccagc tacaattgca tcagcgccaa tgcgcatgcg ctcgcgcgct acgccgcgct
    22141 gtgccaggag gaagacctgg tgccgatcgt ggagcccgaa gtgctcatgg acggcgatca
    22201 cgacatcggc cgctgcgagg aagtcaccga attcgtgctg aagacggtgt tccaggagct
    22261 ctactacgcg cgggtgaagc tcgaaggcat ggtgctcaag cccaacatgg tcgtgcccgg
    22321 gaagaaaagc acgaaacagg catccgacac cgaaattgcc gagaggaccc tgcgcgtgct
    22381 gaaggcttgc gtaccggccg ccgtccccgg catcgcgttt ctgtcgggcg gccagtccga
    22441 cgtcgaggcg acggccaggc tcaatgtgat gaacgcggtc ggcggcgtgc cgtggcggct
    22501 cagcttctcc tacgggcggg cgcttcaagc ggcaccgcag aaggcgtggt cggggagggc
    22561 tgaaaacaag gcggcggcgc aggccgcctt cgcccatcgc gccgagatga atgggctcgc
    22621 aacgctcggg aagtggtcgc ccgagcttga gaagagggcc gcctaggcac ggcaacgaag
    22681 cggaacgcgt cgagcaggcc aaaaaagcca atggtcggtc agcc
//
LOCUS       DASUQP010000194         8928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_777861,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000194 DASUQP010000000
VERSION     DASUQP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777861"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFY74_08710"
     CDS             <1..626
                     /locus_tag="VFY74_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931811.1"
                     /translation="HDIEENVDTEVSAAECADNLDRMLRIEKELRVDATYNMLGILLA
                     RKRQIIEASNPRHSIAFHSFNHSLNDMTQLRQCRDVDLRVRGYRPPRSRITAELSDYN
                     LTFWNFEWLASSVFSLGSEACRIENGLVKIPIAIDDYPLVTRAVDYQDWEPICSSARA
                     RKSFSVSGYTIATPAFGWRGIRSCCTSLRRSAIRKRRSAVRLDVPYA"
     gene            complement(723..1217)
                     /locus_tag="VFY74_08715"
     CDS             complement(723..1217)
                     /locus_tag="VFY74_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931812.1"
                     /translation="MDAAANFDVGVLDVLRRELPGYRFIGSGPADTALRHAFDSWLDY
                     GQPHATYVSRAFERPLAVVAGWPESMGMALAEAQVAGACIVASAHQVSHQVLVPDAAV
                     DYEAGDVASLARALRIAKTRDPARIRERARQHFALRQRCRANARSDRALACFKSTVVG
                     HLPM"
     gene            complement(1823..2086)
                     /locus_tag="VFY74_08720"
     CDS             complement(1823..2086)
                     /locus_tag="VFY74_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931813.1"
                     /translation="MSILAVVLLGAASLASPSFAVEPDVDSPEGPELPAPRYDRPDFQ
                     FDDQAGAPRERDIEGTELGAGPEIIIPEEEEVLTVPPPALPEY"
     gene            complement(2485..2727)
                     /locus_tag="VFY74_08725"
     CDS             complement(2485..2727)
                     /locus_tag="VFY74_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931814.1"
                     /translation="MIQVSILMVRCPSTGRELSTGIEMDAATFAKLPDILSQMKCPAC
                     GLDHNLSTRDAWLGKPAPSAPEFTWLFINNGTLPYD"
     gene            complement(2811..2975)
                     /locus_tag="VFY74_08730"
     CDS             complement(2811..2975)
                     /locus_tag="VFY74_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931815.1"
                     /translation="MKTLISMAAMALTLAVTSPAFAGDVTAATDKASCDKAGGAWDDA
                     TKKCSEKKSY"
     gene            3175..7536
                     /locus_tag="VFY74_08735"
     CDS             3175..7536
                     /locus_tag="VFY74_08735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5931816.1"
                     /translation="MPFDLLHHAVRAWFDQSFAKPTPAQADSWPAIKAGHDVLIAAPT
                     GSGKTLAAFLCAIDGLVRQGLDGGLKDETQIVYVSPLKALSNDIHRNLEVPLAGIRAV
                     LRDRGRPDVAIRSWVRTGDTPQAERQRAAKLPPHIVVTTPESLYILLGSVSGRAMLKT
                     TQAVIVDEIHAVASSKRGSHLSLSLERLSALCGGRLQRIGLSATQKPIEDVARFLVGA
                     DRDRPLIVDSGHFRARDLQLEVPPSPLEAVMSAEVWDQVYDRLAELIESHRTTLIFVN
                     TRRMAERVTRKLSDRLGEVRVAAHHGSLAKEQRLDAERRLKSGALSAMVATSSLELGI
                     DIGDVDLVCQLGSPRSIAAFVQRVGRSGHNVGGQPKGRLFPLSSDDLVECAALVDSVN
                     RGELDRLIMPVAPLDVLAQQIAAEVNAQDWDEDALFALIRRAHPYRDLDRKTYIAALR
                     MLGGGFTTRRGRRGALIHRDEVNGRLRGRRGARLTALTSGGTIPDNSDFQVLLEPENV
                     VVGTVNEDFAVESLAGDIFQLGNTAYRIIRVERGVVRVEDAKGAPPTIPFWLGEAPAR
                     SEELSQSVSRQHAEIEGRLKRDGAAGALAWITDDLGLERAAAEQIVDYLGAAHNALGA
                     LPTRDTLVFERFFDEAGGMQLVIHSPYGSRINRAWGLALRKRFCRKFNFELQAAATED
                     SIVLSLTTAHSFDLAEVARYLNSKTVRELLVQALLDAPMFGTRWRWVSNVALALPRFR
                     GGKKVPPQLMRMMAEDLVGTIFPDQVACAENLVGEREIPDHPLVAQTISDCLNEVMDI
                     EGLERLLGDIESGKIRIVARDLTEPSPLAREVLTARPYAYLDDAPLEERRTQAVMSRR
                     WLDPDTADEIGRLDEAAIARVREEAWPDASNPDELHDALVWLGFLTEAEADSNAAWKD
                     WLVSLAQSGRATRLAAQGRAFWVSAERLRQFQAIFPDAVMEPAITPPASADQVFEPEA
                     ALVEVVRGRLEGQGPTTVVRLGEALGLPTDQISAALAALEVEGFALAGSFTQSGEREW
                     CERRLLARIHRYTVKRLRAEIEPVSARDYLRFLFDWQGVTADSRREGGQALDAVIEQL
                     AGFEAPAAAWEDAILPARLSDYEPSLLDDACLSGRVAWARLGAPTGKPKRNGRRSTPL
                     KSTPISLFPRNEAALWARPLADETLHVSARAVKVVEFIRANGASFFDEIVEGTHLLRT
                     QVEEALAELVAAGEVVSDSFGGLRALLNPPRHHKRRPKGGRLATAGRWTLTKRKLTKS
                     APKNDERVQQTAIALLRRYGVVFMRMLDREASWLPKWRDLLRVYRKLEARGEIRGGRF
                     VSGFSGEQFALPEAVASLRSVRRRAPDDSLVSVSGADPLNLAGILTPRPKLAALAGNR
                     VLYRDGIPIAFLEGDSTRFLEPVEPEIENMARTALHGHAEPAAHFPRLRRLAQLKAAQ
                     ALAEERRGQRRVKPFAA"
     gene            complement(7768..7977)
                     /locus_tag="VFY74_08740"
     CDS             complement(7768..7977)
                     /locus_tag="VFY74_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931817.1"
                     /translation="MPGKRVQFDDETWQAIQALAHQTGKSFQELASMAFGELLKKHDQ
                     PVGFKAELDASVAPRSKVKRVARRG"
     gene            8334..8834
                     /locus_tag="VFY74_08745"
     CDS             8334..8834
                     /locus_tag="VFY74_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX5931818.1"
                     /translation="MAKELKQLDDLFHDTLKDLYFAEKKILAALPKMAKASQSEDLVA
                     AFEKHEQETEGQVQRLEKVFALIDETPRGKNCPAILGIIEEGKEVMDEYKDSPALDAG
                     LLAAAQSVEHYEISRYGTLLAWANELGLEDAVPLLEATLEEEKATDEALTRLAETAIN
                     QQAEAA"
BASE COUNT         1635 a         2834 c         2738 g         1721 t
ORIGIN      
        1 ttcatgatat cgaggaaaac gtcgataccg aggtttcggc ggccgaatgc gcggataatt
       61 tggaccgcat gttgcggatc gaaaaagagc ttcgtgtcga tgccacctac aatatgctcg
      121 gcattcttct tgcgcgaaaa aggcaaatca tcgaagcatc caatccccgt cattccatcg
      181 cgttccattc cttcaatcat agcctcaacg acatgacgca gcttcgtcaa tgccgtgacg
      241 tcgatctccg cgtcagaggc tataggccgc ctcgctccag gatcaccgcg gaattgtcag
      301 actacaacct cacattctgg aacttcgaat ggctcgcttc ttccgttttc agcttgggct
      361 ccgaggcgtg caggatcgag aatggactcg tcaagattcc gatcgctatc gacgattacc
      421 cactggtcac ccgcgcggtt gactatcaag actgggaacc gatttgctcg agcgcgcgcg
      481 cgcgaaaaag tttttcggtt tcgggttaca cgattgctac gccggccttt ggctggagag
      541 gtatccgcag ttgctgcaca agcttgcgca gatcggcgat tcgtaagcgc cgatcagctg
      601 tgcgattgga tgttccttac gcatgacggc gcggcagccg aacccggcat cgatacgggg
      661 aagcgccgcg catgggtgcg tgtggcagat tggctcctgc cctcatcacc gtgatcatcc
      721 catcacatcg gcaaatgtcc gaccaccgtg gacttgaagc aggctaaagc ccgatcgctg
      781 cgcgcgttcg cgcgacaacg ttgtcgaagg gcaaagtgct gtcgtgcccg ctcccgaatc
      841 cgagcgggat cgcgggtttt cgcaatcctg agcgctcggg caagcgacgc gacgtcgccg
      901 gcttcatagt cgactgccgc atcgggcacg agaacctggt gcgacacttg atgggcggat
      961 gcaacaatgc aagctccagc gacctgtgcc tcggcaagcg ccatgcccat cgattctggc
     1021 catcctgcca ccactgcaag cggtcgctcg aaagccctgc tgacataggt cgcgtgaggc
     1081 tgtccgtagt cgagccagga gtcgaacgca tgacgcagcg ccgtgtcggc gggtccggag
     1141 ccgatgaacc gatatccggg aagttcccgg cgcaacacgt caaggacgcc gacgtcgaaa
     1201 ttcgcggctg cgtccataac gttgcttcgc ggaaagtcga acaacacatg ctcacgacga
     1261 ccatccgccg ccggccgcac cacctcggga tcgactccga cccgatccag tgctcgtatg
     1321 gttcgaggtc atcagtgatt atgtggcaga ggaccccgcc catggaggcg aaccattgac
     1381 agacttcata cgaaaagtga tcgggacggc cgtgaagcga tgcgtccaca cgacaaaaat
     1441 atcgatcagc acgaatgtca ttgagcggcc cagcaagtct gcgtgcgaac caccccttat
     1501 cgctttgata gtcgctgcag atgagtacct tgcacgtgaa acccggcaga gggagctgac
     1561 gaaactcggc tcgcagtccg gcgcgcgcga ggtgacgagc gagataaaaa cctccacctg
     1621 cggaaacgac gaaacgctgt cgccgtgcgg ggaacaggtc accaagacgt catcttggag
     1681 gaccggcgaa ggattttcat ccttcttcat atcaggggtc tagggtgaca accagattgc
     1741 gcggggacag gagcgctgac gcggcgagag tagcctccaa cgcatgttgg tttgcccgca
     1801 cttaacgcaa gcgtgggtcc tgctagtact caggaagtgc cggcggcggc accgtcaaaa
     1861 cttcttcctc ttcggggatg atgatttccg gacctgcgcc gagttccgtg ccttcgatgt
     1921 cgcgttcgcg gggcgctccc gcttgatcgt caaactggaa atccggccgg tcatagcgag
     1981 gcgccggaag ctcaggccct tcaggagaat cgacgtctgg ctccacggca aaactcggcg
     2041 aggccagtga ggctgcgccc agaaggacga cggcgagaat gctcattttt acgcttctca
     2101 tactggatgt cctttccttt cgtatcgtac ccccagaggc gaacgaaagt ccggggatat
     2161 cggttcccgc ggttcacgac atttctattg tttggcttct ttcttcccag ccccagcgat
     2221 cgcaaatgcg gcttggagcc tgcaaaaagg aaagccccgg ctggggtgtg cggggctttc
     2281 catattcaga ttcggcgagg atgcgggggg tcgtcaggat aggaatcgcc gaattctatc
     2341 gctacaaacg cgcgcgaggc gcggaggttc ccaagtgcgc cggagagaca gggagcgcac
     2401 tgcccttggc tggcgccacc gcttcctgcc cgccggaacg ctagcgtgca aaccaaagcg
     2461 caacgaccgg gtttgccgtc ggggctagtc gtaaggcaga gttccgttgt tgataaataa
     2521 ccaggtgaat tccggcgcgg agggagctgg cttgccaagc caagcgtcgc gcgtggacaa
     2581 gttgtggtcg aggccgcaag ccggacactt catttgtgac agaatatcgg gaagcttggc
     2641 gaatgttgcg gcatccattt caatgcccgt tgagagttcg cgcccggtgc tagggcacct
     2701 aaccatgagg atgcttacct gaatcatcgg gcccccctcc gagccagtcc gacgagcgca
     2761 cagcaaaagg gcgccaacca aagccgacgc ccgatgaact ccagtcagac ttagtagctc
     2821 ttcttctccg agcacttctt cgttgcgtca tcccacgcgc cgccggcctt gtcgcaactc
     2881 gccttgtcag ttgctgccgt cacgtcgccg gcgaaggcag ggctggtcac ggcaagtgtc
     2941 aaagccatgg cagccatcga gataagagtt ttcatgacgt tgctcctgaa acgctgttac
     3001 tgctgcgttg cgtaactcag gaaaacgccc aattcatcag cccggttcca cgcttgtgca
     3061 gggagggtct ccacctcccg cctaacccat tgagctctag ttgattcgcc agaaaaagcg
     3121 cttatttttg ttttagccgg tcgacctttt cggctgtcaa cctcggctaa ccccatgcca
     3181 tttgacctcc tccaccacgc tgtgcgcgcg tggttcgacc aaagctttgc caagcccacg
     3241 ccggcacagg ccgattcctg gcctgcgatc aaggccgggc atgacgtgct tattgccgcc
     3301 cctaccggct cgggcaagac gctcgcagcc ttcctctgtg ccatcgacgg ccttgtgcgc
     3361 caggggcttg acggcggact caaagatgag acgcagattg tctacgtttc gccgctgaag
     3421 gcgctctcca acgatatcca ccgcaatctc gaagtcccgc ttgccggcat ccgcgcggtt
     3481 ttgcgcgacc gcggtcggcc cgatgtcgcg atccgctcct gggtgcgcac cggtgacacg
     3541 ccgcaggccg agcgccagcg cgcggcgaag cttccaccgc atatcgtggt gaccacgccg
     3601 gaatcccttt acatcctgct tggctccgta tccggccgcg ccatgctcaa gaccacgcag
     3661 gcggtgatcg tggacgagat tcacgccgtt gcgtcgagca agcggggctc acatctttcg
     3721 ctttcgctcg agcggctttc cgcgctttgc ggcgggaggt tgcagcggat cgggctttcg
     3781 gccacgcaga agccgatcga agacgttgca aggttcctgg tcggggccga tcgcgacagg
     3841 ccgctgatcg tggatagcgg ccacttccgg gcccgcgacc ttcagctcga agtgcccccc
     3901 tccccgcttg aagcggtgat gtcggcggag gtctgggacc aggtctacga ccggctcgcc
     3961 gagctgatcg agagccaccg aaccaccctt atcttcgtca acacgcggcg catggccgag
     4021 cgggtgaccc gcaagctctc ggacaggctg ggcgaagtgc gcgtcgccgc ccatcacggg
     4081 agcctcgcca aagagcagag gctcgacgcc gagcggcggc tgaagtcggg agcgctgtcc
     4141 gcaatggtcg cgacatcctc actcgagctc ggcatcgata tcggcgacgt cgaccttgtg
     4201 tgtcaactcg gctcgccccg ctcgatcgcc gccttcgtgc agcgggtcgg ccgttcgggc
     4261 cacaacgtcg gcggccagcc gaagggccgg ctctttcctc tttccagcga cgaccttgtc
     4321 gaatgcgcgg cgcttgtcga cagtgtcaat cgcggcgaac tcgaccggct gatcatgccc
     4381 gtggcgccgc tcgacgtcct cgcccagcag atcgcggcgg aggtcaatgc gcaagattgg
     4441 gacgaggacg cgcttttcgc gcttattcgg cgtgcccatc cgtatcgcga tctcgaccgc
     4501 aagacctata ttgccgcgct tcgcatgctc ggcggcggct tcaccacccg ccggggacgg
     4561 cgcggcgcgc tgatccatcg cgacgaggtc aatggtcggc tgcgcggccg gcgcggcgcg
     4621 cggctgacgg cgctgacctc cggcggcacg atccccgaca attcagattt ccaggtcttg
     4681 ctcgagcccg agaacgtggt cgtcggtacg gtcaacgagg attttgcagt cgagagtctg
     4741 gcgggcgata tcttccagct cggcaatacc gcctaccgca ttatccgcgt ggagcgtggg
     4801 gtcgtcaggg tggaggacgc gaaaggcgcg ccccccacga tcccgttctg gctcggcgaa
     4861 gcgcccgcgc gtagcgaaga gctatctcag tcggtgtcgc gtcagcatgc agagatcgag
     4921 ggccgcctca agagggacgg cgcggccgga gcacttgcct ggatcacgga cgatctcgga
     4981 ctggagcgcg ctgcggccga gcagatcgtg gactaccttg gcgctgccca caacgctctc
     5041 ggcgcgctgc cgacccgcga cacgctcgtc ttcgagcgct tcttcgacga ggccggcggc
     5101 atgcagcttg tcatccattc cccctatggc agccgcatca atcgcgcctg gggcctcgcg
     5161 ctccgcaagc gcttctgccg caagttcaat ttcgagctcc aggcggcggc gaccgaggat
     5221 agcatcgtgc tgtcgctcac cacggcgcac agtttcgatc tcgccgaggt cgctcgttat
     5281 ctgaattcga aaaccgtgcg cgagctgctc gtgcaggcgc tgctcgacgc gccgatgttc
     5341 ggtacgcgct ggcgttgggt ttcgaacgtc gcgctcgctc tgccgcgctt ccgcggcggc
     5401 aaaaaagtgc ccccgcagct catgcgcatg atggcggagg atctcgtcgg cacgatcttc
     5461 cccgaccagg tcgcctgcgc cgagaacctc gtcggcgagc gcgaaatacc cgatcaccca
     5521 ctcgtcgcgc agacgatctc cgactgcctt aacgaggtca tggacatcga gggactcgag
     5581 cgcctgcttg gcgatatcga gtccggaaag atcaggatcg tcgctcgcga tctgaccgag
     5641 ccttcgccgc tggcgcgtga ggtgctgacc gcgcggcctt acgcctatct cgacgatgcg
     5701 ccgcttgaag aacgccgtac gcaagcggtc atgagccgcc gatggctcga tcccgatacg
     5761 gctgacgaga tcggccgcct cgacgaggcc gcgatcgcgc gcgtgcgcga ggaggcctgg
     5821 cccgatgcgt cgaacccgga cgagctgcac gatgcgctgg tatggctcgg gttcctgacc
     5881 gaagccgagg ccgattcgaa cgcggcttgg aaggactggc tcgtttcgct agcgcagtcc
     5941 ggccgcgcca cgcgcctcgc cgcgcaaggt cgcgcgttct gggtttcggc cgagcgcctc
     6001 cgccagttcc aggcgatctt tcctgacgct gttatggagc cggccataac gccgccagcg
     6061 agcgcggacc aggtttttga gcccgaggct gctctggtcg aggtcgtgcg cgggcggttg
     6121 gaaggccagg gcccgacaac ggttgttcgg ctgggcgagg cgctcggcct gcccaccgat
     6181 cagatttcgg ccgcgctcgc cgcgctcgaa gtcgagggct tcgccttggc cggctccttc
     6241 acccaaagcg gagagcgcga atggtgcgag cggcgcctgc tcgcgcgcat ccaccgctac
     6301 acggtaaagc gacttcgcgc cgagatcgag cccgtctccg cgcgcgatta tctccgcttc
     6361 ctgttcgatt ggcagggcgt cactgccgac tcacggcgcg aaggggggca ggcgctcgat
     6421 gccgtgatcg agcagcttgc aggcttcgag gcgcctgctg ccgcgtggga ggacgccatc
     6481 cttccggcgc gtctctccga ttacgaaccg agcttgctcg acgatgcctg tctctcgggg
     6541 cgagtcgcct gggcgcggct cggagcaccg acaggcaagc cgaaacgaaa cggcaggcgc
     6601 tctaccccgc tgaaatcgac gccgatcagc ctctttccgc gcaatgaggc cgctctgtgg
     6661 gcgcgtccgc tcgcggatga gacgctccat gtcagcgccc gggcggtcaa agtcgtggag
     6721 ttcatccgcg caaacggtgc ctctttcttc gacgagatcg tcgagggcac gcatcttctc
     6781 cgcacgcagg tcgaggaagc gcttgccgag ctcgtcgcgg ccggggaggt tgtttccgac
     6841 agtttcggcg gcctgcgcgc gctccttaac ccgccccgcc atcataagcg ccggccgaag
     6901 ggcgggcgtc tcgcaaccgc tggcagatgg acgctcacga agcgcaagct tacgaagagc
     6961 gctcccaaaa acgatgaacg cgtgcagcag accgcgatcg cgctcctgcg ccgctacggc
     7021 gtcgtgttca tgcggatgct ggaccgcgag gcctcatggc ttcccaaatg gcgcgatctc
     7081 ctgcgcgttt atcgcaagct cgaggcgcgc ggcgagatca gaggcggacg cttcgtgtcg
     7141 ggtttctctg gcgaacagtt cgcgctgccc gaggctgtgg cctctttgcg cagcgtcagg
     7201 cggcgcgcgc cagacgacag tctcgtttcc gtctccggcg ccgatccgct caacctcgcc
     7261 ggtattctca cgcccagacc gaagcttgcg gcgctcgcgg gcaaccgcgt gctctaccgc
     7321 gacggcattc cgatcgcgtt cctggaaggc gacagcacgc ggttcctcga gccggtcgag
     7381 ccggagatcg agaacatggc gcgcactgcc ctgcatgggc atgccgagcc cgccgcccat
     7441 ttcccgcgct tgcggcgcct tgctcagctg aaagcagcgc aagctttggc agaggaacgt
     7501 cggggccagc gtcgggtcaa accgttcgcg gcttgagtca tcgccgcttt atcgcgtgtt
     7561 cgcttacgcg cacgcgcgca agcgacattc atcgcgattg gcgcgactgt gaccgtgcaa
     7621 cgacctcgtt gatcgttcta atccggagct acgggtggct tgcccttgtt ctcctcacca
     7681 ggggacgtgg tggcggcatt gtcaccggtt ccagctccgc tcatgccgat ctcagcatcc
     7741 ggagacgggg tctggctccg cctgcttcta acccctgcgc gccactcttt taaccttgct
     7801 ccgcggcgcg acgctcgcgt cgagctccgc cttgaagccc acaggttgat cgtgcttttt
     7861 caggagctcg ccgaaagcca tgcttgccag ctcctggaag ctcttaccgg tttgatgggc
     7921 aagggcttga attgcctgcc acgtctcgtc gtcgaattga acacgtttgc ccggcatgca
     7981 ttccacttct tgggttatcg cgtttcgtgg gcgcgtcggc cgcggtcatg tcagttccag
     8041 atcgacacta ggggattcaa gaaccaactt tggctttaac atggggttcc attgacctgt
     8101 ttacattagt ccttcgtcgc acggatgttc gcggcggcct tgcaacttta tcgggccggc
     8161 aaagttcatt cgcccaacgc gcgcgtgttg tgggccccac cgctgttcat tgggcgcgag
     8221 gctcgggccg gcgccaaccc ttctggaaaa aacttcgtcc taaaaagcca gggtctcgca
     8281 accttttggc tcgatcgggg ttcgaaccaa aaccctataa cggagatctc cccatggcca
     8341 aagagctcaa gcaactggac gacctttttc acgacacgct caaggatcta tatttcgcgg
     8401 agaagaaaat tctggccgcg cttccgaaaa tggcgaaggc ttcacaaagc gaagatttgg
     8461 ttgccgcgtt cgagaagcac gagcaggaaa cggaggggca ggtgcaacgc ctcgaaaagg
     8521 tttttgccct gatcgacgaa acgccgcgag gtaagaactg cccggccatc ctcggcatca
     8581 tcgaggaggg caaggaagtg atggatgagt ataaggactc gcccgcgctc gacgcgggac
     8641 tgctagccgc ggctcaatcc gtcgagcact atgaaatctc ccgctacggc accttgctcg
     8701 cttgggctaa cgagctcgga cttgaagacg ccgttccctt gctcgaagcc actctcgaag
     8761 aagaaaaagc aacggatgaa gctttgacgc gtttggctga gacagcgatc aaccaacagg
     8821 cggaagccgc ttaatcttga gcctgtggca ttgtccttaa tcgtgcgccc aggcgtctcc
     8881 ttgtgtggcg gcggcctcgc gcgcccgatt ggcagcgcgt tcgagatc
//
LOCUS       DASUQP010000195         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_779030,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000195 DASUQP010000000
VERSION     DASUQP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779030"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..888
                     /gene="cysW"
                     /locus_tag="VFY74_08750"
     CDS             4..888
                     /gene="cysW"
                     /locus_tag="VFY74_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX5931819.1"
                     /translation="MSDLVYGLDFVTADRARSVTTERRPVRIALILTAVTFLTLFLLL
                     PLFAVFSEAFRAGFGGFWRAVSEPDAVAAMKLTLLVAAIAVPANLIFGLAASWAIAKF
                     EFPGKSILNTLIDLPFSVSPVISGLVYVLLFGLQGYFGPYLLSQGIQIIFAVPGIVLA
                     TIFVTFPFVARELVPLMQEQGTQDEEAALSLGASGFKTFLLVTLPNVKWALLYGVILC
                     NARAMGEFGAVSVVSGHIRGKTNTMPLHVEILYNEYNFAAAFAVASLLALLALVTLAV
                     KIALEWRYADQIAGSARH"
     gene            1006..2058
                     /locus_tag="VFY74_08755"
     CDS             1006..2058
                     /locus_tag="VFY74_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX5931820.1"
                     /translation="MHVTVEDVVRTFGLTPALHGVSLDIYPGELVALLGPSGSGKTTL
                     LRILAGLDIPTAGRVLFDQDDALKLTVQERHVGLVFQNYALFRHMTVAENIGFGLSVR
                     PRSRRPSRKEIARRALDLLELVQLSGLEKRYPSQLSGGQRQRVAFARALAIEPRLLLL
                     DEPFGALDAKVRRDLRRWLREIHDKTGHTTVFVTHDQEEALELADRVVVMSQGRIEQV
                     GTPDEIYDEPNSPFVFSFIGESSELPVHIEQGTIVLDEMPLALAGQDCPDGPARLFFR
                     PQDVEAGSGLPGAIPGTIAAIRRHGGARRLDVELGREGHRIEIELPAQFDASIGGGIA
                     VFPRRWRLYPDRPADR"
BASE COUNT          367 a          736 c          692 g          444 t
ORIGIN      
        1 gtcatgagcg acctcgtcta cggtctcgac ttcgtcaccg cggatcgcgc gcgctccgtc
       61 acgaccgagc ggcgtccggt ccgcatcgcc ttgatcctca ccgccgtcac cttcttgacc
      121 ttgttcctgc ttctgccgtt gtttgccgtg ttctccgagg cgtttcgcgc cggcttcggc
      181 ggtttttggc gcgccgtctc cgaacccgac gcggtcgcgg caatgaagct cacgctgctg
      241 gttgccgcca tcgccgtgcc cgccaatttg atcttcgggc tggccgcatc atgggccatc
      301 gccaagttcg agtttcccgg caagagcatc ctcaacaccc tcatcgacct gccattctcc
      361 gtgtctccgg tgatctcggg gctcgtctac gtgctcctgt tcgggctgca aggctatttc
      421 ggcccatacc tgctaagcca aggcatccag atcatcttcg ccgtcccggg catcgtgctc
      481 gccaccatct tcgtaacctt ccctttcgtc gcgcgcgagc tcgtcccgtt gatgcaggag
      541 caggggacgc aggacgagga agccgcgctc tcgcttggcg cctccgggtt caagacgttc
      601 ctccttgtga ccttgcccaa cgtgaaatgg gcgctgcttt acggcgtcat cctctgcaac
      661 gcccgcgcca tgggggagtt cggcgccgta tccgttgtct cgggccatat cagaggcaag
      721 accaatacga tgccgctgca tgtcgagatt ctgtacaacg agtacaactt tgctgcggcc
      781 tttgccgtgg cgtcgttgct cgcgctgctc gctctcgtca ccttggcggt gaaaatcgcc
      841 ttggagtggc gctacgccga tcaaatcgca ggatcggcgc ggcactgagc gagaaaaagg
      901 gaggaaatca tggacgctcg actggattat aatacgaacg gcgcggccgc gggcaccttg
      961 caacccgtgg cggagcgcac gcgcgcggga ggctgggaca acccgatgca tgtcacggtc
     1021 gaggacgtcg tcaggacatt tggtctgacg cctgcgctcc atggcgtctc gctcgacatc
     1081 tatcccgggg agctcgtggc gcttctcggg ccctctggct ctggcaagac cacgctgctg
     1141 cgcatccttg ccggtctcga tattccgaca gcggggcggg ttctgttcga tcaagacgac
     1201 gcgctgaagc tcaccgtgca ggagcgccat gtcggccttg tgttccagaa ttacgcgctc
     1261 ttccgccaca tgacggtcgc cgagaatatc ggcttcggtt tgagcgtgcg cccgcgctcg
     1321 cgccgcccga gccgcaagga gattgcgcgg cgcgcgcttg acctgctcga gctcgtccaa
     1381 ctctctggac tcgaaaagcg ctacccgtcg cagctctcgg gaggccagcg gcagcgcgtc
     1441 gcctttgccc gcgcgctcgc tatcgagccg cgcctgctcc tgctcgacga gccgttcggc
     1501 gccctcgacg ccaaggtgcg ccgcgacctc cgccgctggc tccgcgaaat ccacgacaag
     1561 accggtcaca ccacggtgtt cgtgacccac gaccaggaag aggcgctcga acttgccgac
     1621 cgggtggtgg tgatgagcca agggcgcatc gagcaggtcg gcacgccgga cgagatctac
     1681 gacgagccga attcgccctt cgtattctcg ttcatcggcg aatcgagcga attgcccgtg
     1741 cacatcgagc aagggacgat tgttcttgac gaaatgccgc tcgcgcttgc cgggcaggac
     1801 tgtccggacg gtccggcgcg actgttcttt cgtccgcagg atgtcgaggc ggggtccggc
     1861 ctcccaggcg cgatcccggg cacgattgcg gcaatccgcc ggcatggcgg cgcgcggcgg
     1921 ctcgatgttg agctcggtcg ggaaggtcac cgcattgaaa tcgagctgcc agcgcagttc
     1981 gacgcaagta tcgggggagg gatcgccgta tttccccgcc gttggcggct ctatccggat
     2041 cggccagccg accgataagg agtgtgggcg aagccgacgc cctccgccga accggagaac
     2101 gtttttgcca aactcaggtc ataaaggaaa ccgcgtcaag gagatctact acgcgggggt
     2161 ttgggcttcg aacgtgaact tcggctcagg tccgaacggg caggatctcg ccagcatcga
     2221 aatcgactac tacgccggc
//
LOCUS       DASUQP010000196         8345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2006238, whole genome shotgun sequence.
ACCESSION   DASUQP010000196 DASUQP010000000
VERSION     DASUQP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006238"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFY74_08760"
     CDS             complement(<1..281)
                     /locus_tag="VFY74_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HEX5931821.1"
                     /translation="MSEDTGTRAFAPFEWVVALRYLRARRKEGFISVIAGFSFIGIML
                     GVATLIIVMAVMNGFRAELLNKILGFTGHINVYATGAAPVSNFDELAQS"
     gene            complement(278..1627)
                     /locus_tag="VFY74_08765"
     CDS             complement(278..1627)
                     /locus_tag="VFY74_08765"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5931822.1"
                     /translation="MRLSRYFLPLLRDDPKEAEIASHRLMLRAGLIRQASAGIYSWLP
                     LGFRVLRKIEAIVREEQDRAGAIELLMPTIQPAELWQESGRYEVYGKEMLRISDRHER
                     AMLYGPTNEELITAIFRDAVKSYKDLPRILYHIQWKFRDEIRPRFGVMRGREFLMKDS
                     YSFDLTVEDARKSYNRMFVSYLRTFARMGLKSVPMIAETGPIGGNLSHEFIILADTGE
                     SEVYCHRDLIALDPPGADIDYDGDLSPVVDAFTGKYAATSEKHDADRFAREVAEKDRV
                     TARGIEVGHIFFFGTKYSEAMGCKVQGPGGELVTVQMGSYGVGISRLVGAIIEASHDD
                     AGIIWPQAVAPFDIGLINVKAGDAATDAACEGLYGKLEAAGLSVLYDDRDDRAGAKFA
                     AMDLIGLPWQIVVGPKGVASGEVELKERATGARHSLTFEAALNRLLQSASEASRKAA"
     gene            complement(2190..2468)
                     /locus_tag="VFY74_08770"
     CDS             complement(2190..2468)
                     /locus_tag="VFY74_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEX5931823.1"
                     /translation="MTLAFALALYFIIWWIVLFAMLPIGVRTSEEAGEKASPGTAESA
                     PHMPNLLPKMVATTVVSSIIFAALYAVIVHRVITLDDIPFFPRYERVQ"
     gene            complement(2472..2876)
                     /gene="mce"
                     /locus_tag="VFY74_08775"
     CDS             complement(2472..2876)
                     /gene="mce"
                     /locus_tag="VFY74_08775"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX5931824.1"
                     /translation="MIGRLNHVAIAVPDLAAATQVYRDTLGARVSDAVDQPEHGVTTV
                     FIELPNTKIELLGVFGEGSPIAGFLERNPNGGIHHLCYEVPDILVARDRLVAQGARVL
                     GQGEPKIGAHGRPVLFLHPKDFCGTLVELEEA"
     gene            complement(2916..4601)
                     /locus_tag="VFY74_08780"
     CDS             complement(2916..4601)
                     /locus_tag="VFY74_08780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5931825.1"
                     /translation="MSRKSHAKGVDFVFLPLGGVGEIGMNLYLYGYGSEGAREWLIVD
                     MGVIFGGEAEPGIDVILPDIRFIEEERHNIVGLLLTHAHEDHFGAVVDLWPLLSGVPV
                     YATPFTAEMLKSKLAEIGLVNGFPLQIIPLGDRRTIGPFDVELINMSHSIPEPSAVVI
                     RTPLGAALHTGDWKLDDNPLTSAPTDAARLKALGENGLAALICDSTNAIRDGVSPSEA
                     DVAVVLARLIREAPNRVAVTTFASNVARLGSVAKAARAAGRELVVVGRAMLRVIEAAQ
                     ATGYLDPNLHFHDETAFARLPPRKVVALCTGSQGEPRAALARIAQGEHPNVELEEGDQ
                     VIFSSRTIPGNEKAVSRVQNGLADLGVEVITDQDAPVHVSGHPRRGELEQLYGWLKPG
                     IAIPMHGEGRHLEAHAKLAERLGVKHVVRARNGAMVRLLPGPAEIIDDVPVGRLYRDG
                     MILTRADDGQVRERRKLSFAGSVAVSLVLSTKGVLLAEPEIALTGLPSADTQGTPLAE
                     IAREAVIGTLESIPRPKRKDQALVSDAVRRGIRAAVNQAWGKKPMCSVLVTML"
     gene            complement(4616..5395)
                     /locus_tag="VFY74_08785"
     CDS             complement(4616..5395)
                     /locus_tag="VFY74_08785"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5931826.1"
                     /translation="MASFSTSLPRLPSGYRLLRMEAVDSTNAEAKRRARAGEPGPLWI
                     WSARQSQGRGRGGREWISRPGNLFASLLIGINSPPRVAGQLALIAGIVAYDTIAKLIA
                     YEGRSELLLKWPNDILLSGEKLAGVLLEHIGSQIENRSVVVIGTGINLASYPEDLPQP
                     AVSLAAYGMEVTPGDALEVLAATTHEWLARWGEGSCFPTIRRAWLDRAGPAGRPLRVK
                     INDEETEGVYGGLDADGALRLLTPDGAEHRIAAGDVFFLSS"
     gene            complement(5396..6826)
                     /gene="nuoN"
                     /locus_tag="VFY74_08790"
     CDS             complement(5396..6826)
                     /gene="nuoN"
                     /locus_tag="VFY74_08790"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX5931827.1"
                     /translation="MPHADNLDVALPEIILACGAMALLMLGVFTRKARGELILWLAVL
                     LMAVAGVFVASTSGTATTFGGSFIVDPFARMLKLLTLAGGAVALLMSIDYWRGGGGLK
                     FEYPVLVLFAMTGMLMMISANDLIALYVGLELQSLALYVVAAFRRDSARSAEAGLKYF
                     VLGALSSGMLLYGASLVYGFTGSTSFAVIAAAVQPSGANIGLIFGLVFLMAGFAFKIS
                     AVPFHMWTPDVYEGAPTPVTAFFAAAPKLAAMALTVRVLITAFPAVTTEWRQIVVFLA
                     IASMALGAFAAIGQTNIKRLMAYSSIGHMGYGLVGLAAGSPDGVAGVVFYLAIYLAMT
                     LGTFACILAMRRNGRMVESIDALSGLSRTNPAMAFLLAMLLFSLAGIPPLAGFFAKFY
                     VFLAAIHAGLYALAVIGVLLSVVGAYYYLRIVKIMYFDAPAESFEPMPVTLAAALGVS
                     GIFVLVYSVYPAPLVAAASQAAKSLF"
     gene            complement(6839..>8345)
                     /locus_tag="VFY74_08795"
     CDS             complement(6839..>8345)
                     /locus_tag="VFY74_08795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5931828.1"
                     /translation="SWPILSTVTFLPLLGALLIVALRGNDEAASRNARFIALWTTLVT
                     FALSLILWRDFDPLTAQFQFVELRPWLGPISFHMGIDGISLPFVLLTTLLMPLCILAS
                     WVPITERVKEFMIAFLVLETLMLGVFTALDLVLFYLFFEGGLIPMFLIIGVWGGTRRV
                     YASFKFFLYTLAGSLLMLLAIMAIYWEAGTTDIPVLLTYGFPAEMQTWLWFAFLASFA
                     VKLPMWPVHTWLPDAHVEAPTAGSVILAAILLKMGGYGFLRFSIPMFPLASAEFAPLI
                     YALSIVAIIYTSLVALAQEDVKKLIAYSSVAHMGFVTIGVFTLTMQGLQGGIFQMLSH
                     GIVSAALFLCVGVIYDRMHTREIAAFGGLADRMPLYAFCFMVFTLANVGLPGTSGFVG
                     EFLSLAGAYRVNTWVAFLATTGVILSVAYALWLYRRIIFGKLEKASLKSISDLTWREV
                     VVMTPLVVLTILFGFYPAPILDVSATSVETLIKGFNASLAAAEQARLALVP"
BASE COUNT         1698 a         2584 c         2749 g         1314 t
ORIGIN      
        1 aaactctgcg ccagctcgtc gaaattgctg acgggcgctg cgcccgtggc atagacgttg
       61 atgtggccgg tgaatcccag gatcttgttc agaagctcgg cacgaaagcc attcatcacc
      121 gccatgacga tgatcaaggt ggcgacgccg agcatgatgc cgatgaagga gaagccggcg
      181 atcaccgaga tgaagccctc cttgcggcgc gcccgcaaat agcgcagcgc taccacccac
      241 tcgaaggggg cgaaggcgcg cgttccggtg tcttcgctca tgcggccttc ctgctggctt
      301 cgctcgcgga ttggaggagg cgattcaacg cggcttcgaa ggtgagacta tggcgggccc
      361 cggtcgcacg ctccttcagc tcgacttcgc ctgaggccac gcccttcggc ccgacgacga
      421 tctgccaggg cagcccgatt aggtccatgg cggcgaattt ggcccccgcc cggtcgtcgc
      481 gatcgtcata gagcacgctg aggcccgcgg cctccagctt cccgtaaaga ccctcgcaag
      541 ccgcatcggt cgcggcgtcg cccgccttga cgttgatgag accgatgtcg aacggcgcca
      601 cggcctgcgg ccagatgatg ccggcgtcgt cgtgggaggc ctcgatgatg gcgccgacaa
      661 gccgcgaaat gccgaccccg taggaaccca tctgcaccgt gaccagctcg ccgccggggc
      721 cttgcacctt gcagcccatc gcttccgagt atttggtgcc gaagaagaaa atgtggccga
      781 cctcgatccc gcgggcggtg acgcgatcct tctctgcgac ctcgcgcgcg aagcgatcgg
      841 cgtcatgctt ctcggaggtg gcggcatact ttccggtgaa ggcgtcgacc acgggcgaca
      901 aatcgccgtc atagtcgatg tcggcgccgg gcggatcgag cgcgatgaga tcgcgatggc
      961 aatagacctc gctctccccc gtgtcggcga gaatgatgaa ctcgtgactg agattgccgc
     1021 cgatcggccc ggtctcggca atcatgggta ccgatttgag acccatgcgg gcgaaggtcc
     1081 gcaaatagga gacgaacatc ctgttgtagg acttgcgcgc gtcctcgacg gtgagatcga
     1141 aggaatagga atccttcatc aagaactcgc gccctcgcat cacgccgaag cgcggcctga
     1201 tctcgtcgcg gaacttccac tggatatgat agagaatgcg cggcagatcc ttgtaggact
     1261 tcactgcgtc gcgaaagatc gcggtgatga gctcctcgtt ggtcggcccg taaagcatcg
     1321 cgcgctcgtg gcgatcgctg atgcgcagca tctccttgcc gtagacttcg tagcggccgc
     1381 tttcctgcca aagctcagca ggctgaatgg tgggcatgag aagctcgatc gcgccggcgc
     1441 ggtcctgctc ctcgcgcacg attgcctcga tcttgcggag aacgcgaaag ccgaggggaa
     1501 gccaagaata gatgccggca ctcgcctggc gaatcaggcc cgcgcgaagc atcagccggt
     1561 gcgaggcgat ctcggcttcc ttcggatcgt cgcggagaag cgggaggaaa taccggctca
     1621 gtcgcatggg caccattttg gcggtttggg gcggatcgcg gggtcgcgct gcccggtgga
     1681 aaacaaccat gccttagcgg aggggcttcc ctccccgccg cccccgtcgc ggctaatagc
     1741 ttactttcgc gcgcataagc aatcctggga aggaggccgg gcggatcgca agattgtcgc
     1801 ctgacacgcc cgcaacggcc gatcgtcgag gagcgttgcc ctcaaaagca ccgtccccgg
     1861 ccacaatcat ggtgacaatt tggcgctgct agagtagggt ccgcgccaca gcgccagaag
     1921 agttcgggac cggtcgcgat ctcgaagtcg ggcgttgaga ttggactcgg tccaagtctt
     1981 gggaggaagt aactccaggc gcgccaatct tgcttcacgc atgggtgcag ccgctccgaa
     2041 gccggcaaat cttgatcgtc atgacgacgc gagccttggt ggcttgggag acaaggagtg
     2101 agttactcaa gcaaggccag ccggccttgc ttttttttgg cctgacccca gcacatgcgc
     2161 cgtttgtcag cagccgtcgc tcaaccgcgt tattgtacgc gctcgtaacg cggaaagaac
     2221 gggatgtcgt cgagcgtgat cacgcgatgc acgatgacgg cgtagagcgc ggcgaagatg
     2281 atgctcgaga cgactgtcgt tgcaaccatc ttgggcagaa gattcggcat atgcggggcg
     2341 ctctcggcgg tccccggcga ggccttctcg cccgcctcct cggaggtgcg gactccgatc
     2401 ggcaacatgg cgaaaagcac gatccaccag atgatgaagt agagcgcgag cgcgaaagcg
     2461 agcgtcatcg cctacgcctc ctcgagctcc accagtgtcc cgcaaaaatc cttggggtgc
     2521 aaaaacagga ccggcctgcc gtgcgcgcca atcttcggtt ccccctgccc cagcacgcgc
     2581 gcgccttgcg ccaccaagcg atcccgcgcg accaggatgt cggggacctc gtagcaaagg
     2641 tgatggatcc cgccattggg gttccgctcg agaaaaccgg cgatcggaga gccctcgccg
     2701 aacacgccga gcagctcgat cttggtattg ggaagctcga tgaacaccgt tgtcacgccg
     2761 tgctcgggct ggtcgaccgc atccgacacg cgcgcgccaa gcgtgtcgcg atagacttgc
     2821 gtcgccgccg cgaggtccgg cacggcgatg gcgacatggt tcaggcggcc gatcattcct
     2881 tgctctccgt tcaacagcga aatttcgcgc gtgttctata acatggtcac aaggaccgag
     2941 cacatcggct tcttgcccca ggcttggttc acggcggcgc ggatgccgcg gcggacggca
     3001 tcgctcacca gcgcctggtc cttgcgcttc gggcgtggga tgctttcgag cgtgccgatc
     3061 actgcttcgc gcgcaatctc ggcgaggggc gttccctggg tgtcggcgga gggaaggccg
     3121 gtgagcgcaa tttccggctc cgccagcaga acccccttcg tcgacaggac cagcgaaacg
     3181 gcgacggatc ccgcgaagct cagcttgcgg cgctcgcgga cctggccgtc atcggcgcgg
     3241 gtgaggatca tgccgtcgcg gtagagccgc ccgaccggca cgtcgtcgat gatctcggcg
     3301 ggtcccggca ggaggcgcac catggcgccg ttgcgcgccc gcaccacatg cttgacgccg
     3361 agcctttcgg cgagcttggc gtgggcctcc agatgacggc cctcgccatg catcggaatg
     3421 gcaatcccgg gcttcaacca gccgtagagc tgctccagtt ccccgcggcg cggatgcccg
     3481 gagacatgca cgggcgcatc ctggtccgtg atcacctcaa cgcccaggtc cgccaatcca
     3541 ttctgcacgc gcgacacggc cttttcgttg ccggggatcg tgcgcgagga aaaaatcacc
     3601 tggtcgcctt cttcgagctc gacattcggg tgctcgcctt gggcgatgcg cgcgagcgcc
     3661 gcgcgcggct cgccctggct tcccgtacag agcgccacga ctttgcgcgg cggaagcctg
     3721 gcgaaggcgg tctcgtcgtg gaaatggaga ttggggtcga gatagccggt cgcctgcgcc
     3781 gcctcgatca cgcgcagcat ggcgcggcca acgacgacga gctcgcggcc ggcagcgcgc
     3841 gcggccttgg cgacggagcc gagacgcgcg acgtttgagg caaaagtggt gacggcgact
     3901 cggttcggcg cctcgcggat cagccgcgct agcaccaccg ccacgtcggc ttctgacggc
     3961 gacacgccgt cgcggatagc attggtggaa tcgcagatga gcgcggcaag accattttcg
     4021 ccgagcgcct tcaggcgggc ggcgtcggtg ggcgcgctcg tgagcgggtt atcgtcaagc
     4081 ttccagtcgc ccgtgtgaag agcggcgccg agcggtgtcc tgatgaccac ggcggacggc
     4141 tcggggatcg agtgagacat gttaatcagc tcgacgtcga aggggccgat ggtgcggcga
     4201 tcgcccaagg ggatgatctg caaggggaag ccgttgacta ggccgatctc ggccagcttc
     4261 gatttcagca tctcggcggt aaagggcgtg gcgtaaaccg ggacaccgga aagaagaggc
     4321 cacagatcga ccaccgcgcc gaaatgatcc tcgtgcgcgt gcgtgagcag gaggcccacg
     4381 atgttgtgcc gttcctcctc gatgaagcgg atatcgggga ggatcacatc gatgccgggc
     4441 tcggcctcgc cgccgaagat gacgcccata tcgacgatca gccattcgcg ggcgccttcg
     4501 cttccgtagc catagaggta cagattcatg ccgatctcgc ccacgccgcc aagcggcagg
     4561 aagacgaaat cgactccctt cgcgtgagat tttcgcgaca tttcgctgat gaggcctacg
     4621 agctcaagaa gaagacatcg ccggcggcga tacgatgttc cgcgccgtcg ggcgtcagca
     4681 gcctgagcgc gccgtcggca tcgagcccgc cgtaaacccc ctccgtctcc tcgtcattga
     4741 ttttcacgcg caagggcctt ccggcaggac cggcgcggtc gagccaagcg cgccggatgg
     4801 tggggaagca ggacccctcc ccccagcgcg caagccactc atgcgtcgtt gcggccagca
     4861 cttcgagcgc gtcccccggc gtcacctcca tgccataggc cgcgaggctc acggccggtt
     4921 gcggcagatc ctcaggatag ctcgcgagat tgatcccggt tccgatcacc acgacggagc
     4981 ggttctcgat ctggcttcca atatgctcga ggaggacgcc ggcgagcttc tcaccggaga
     5041 gcaggatatc gttcggccat ttgagcaaca gctcggagcg gccctcataa gcgatcagct
     5101 tggcgatcgt gtcataggcg acgatgccgg cgatgagcgc aagctgcccg gcgacgcgcg
     5161 gcggagagtt gatcccgatc aagaggctcg caaacaggtt gccggggcgc gaaatccatt
     5221 cgcggccgcc gcggccgcgc ccttgcgact gccgcgcgga ccaaatccac aaggggcccg
     5281 gctccccggc tctggcgcga cgcttcgcct cggcgttcgt cgagtcgacg gcctccatgc
     5341 gcagcaggcg gtagccgctc ggcaatctgg gcaatgaggt tgaaaaagat gccatttaaa
     5401 acagtgactt agctgcctga ctggccgccg ctaccagcgg tgccggatag accgaataaa
     5461 cgaggacaaa gattccgctg acgccaagcg cggcggcgag cgtcaccggc atcggctcga
     5521 aagattcagc gggcgcatcg aaatacatga tcttgacgat gcggagataa tagtaggccc
     5581 ccaccacgct caagagcacg ccgatcacag cgagcgcgta gaggcccgcg tgaatggcgg
     5641 cgaggaacac gtagaacttg gcgaagaaac ctgcaagagg cggaatgccg gcgagcgaaa
     5701 agagcaacat cgccaatagg aaagccatcg ccggattggt gcgggagagg cccgacagag
     5761 cgtcgatgct ttcgaccatg cggccgttgc ggcgcatggc gagaatgcag gcaaacgttc
     5821 cgagcgtcat ggcgagatag attgcaagat agaaaaccac gccggcgacg ccgtcggggc
     5881 tgccggcggc aagcccgacc agtccgtagc ccatgtgccc gatggagcta taggccatca
     5941 gccgcttgat attcgtctgg ccgatggcgg caaaggcgcc gagcgccatc gaggcgatgg
     6001 cgagaaacac cacgatctgc cgccattccg tcgtcaccgc cggaaacgcc gtgatgagca
     6061 cgcgcaccgt gagcgccatg gcggcgagct tgggagccgc ggcgaaaaag gccgtgaccg
     6121 gcgtgggggc cccctcatag acgtcgggcg tccacatatg aaacggcacg gccgaaatct
     6181 tgaaagcgaa acccgccatc aagaacacga ggccgaagat gagcccgata ttggcgccgg
     6241 agggctgcac cgccgcggca attacggcga agctcgtcga gccggtgaag ccgtagacga
     6301 gcgatgcgcc gtaaagcagc atgccggatg agagcgcgcc gaggacgaag tatttgaggc
     6361 ccgcttcagc cgagcgcgcg gagtcgcgcc ggaaggcggc gaccacgtag agcgcgagac
     6421 tctgcaactc aagtccgaca taaagcgcga tgagatcgtt ggccgagatc atcatcagca
     6481 tgcccgtcat ggcgaacagg accagcaccg gatattcgaa tttcagtccg ccccccccgc
     6541 gccaataatc gatcgacatg aggagcgcga ccgcaccacc ggcaagcgtc agcagcttca
     6601 gcatccgcgc gaacggatcg acgataaagc tgccgccaaa ggtggtcgcc gtgccggaag
     6661 tgctcgccac gaaaacgccg gcaacagcca tcagcaggac cgcgagccac aggatgagct
     6721 cgccgcgggc cttgcgcgtg aagacgccga gcatcagaag cgccatggcg ccgcacgcca
     6781 agatgatctc cggcagtgcg acgtccaggt tgtcggcgtg gggcacgagg ggcgctcctt
     6841 acggcaccaa ggcgagtcgc gcctgttcgg ccgcggcaag gcttgcgttg aagcccttga
     6901 tcagggtttc gaccgaggtg gccgaaacat cgagaatggg cgcagggtaa aacccgaaaa
     6961 gaattgtgag cacgacgagc ggcgtcatca ccacgacctc ccgccaggtg aggtcggaaa
     7021 tggatttcag gctcgccttc tccaacttgc cgaagatgat gcggcgatag agccagagcg
     7081 cgtaggccac cgacaagatg acgcccgtgg tggcaaggaa agcgacccag gtgttgacgc
     7141 ggtaggcgcc ggcaaggctc aagaactcgc cgacgaagcc gctcgtgccg ggaagcccga
     7201 cattggcgag ggtgaacacc atgaagcaga aggcatagag cggcatgcgg tcggcgagcc
     7261 cgccgaaggc cgcgatctcc cgcgtatgca tgcggtcgta gatcacgccg acacacaaga
     7321 acagcgcggc cgagacgatg ccgtgcgaca gcatctggaa aatgccgcct tgcaggccct
     7381 gcatcgtgag cgtgaacacg ccgatggtga cgaagcccat atgggcgacg gaggaatagg
     7441 cgatcagctt cttcacgtcc tcctgcgcca gcgccacgag cgaggtgtag atgatggcga
     7501 cgatcgagag cgcgtagatg agcggtgcga actcggccga ggcgagcggg aacatcggga
     7561 tggagaagcg caggaagccg tagccgccca tcttcagcaa gatggcggcg aggatgaccg
     7621 agccggccgt cggcgcctcc acatgggcgt cgggaagcca ggtgtgcacc ggccacatcg
     7681 gaagcttcac ggcaaaggag gcgaggaagg cgaaccaaag ccaggtctgc atttcggccg
     7741 ggaagccgta ggtcagaagc accggaatat cggtcgtgcc cgcctcccaa tagatcgcca
     7801 tgatggcgag cagcatcagc agcgagcccg cgagcgtata gaggaagaac ttgaagctcg
     7861 cgtagacgcg gcgcgttccg ccccacaccc cgatgatgag gaacatgggg atcaggccgc
     7921 cctcgaagaa cagatagaac agcacgagat cgagcgcggt gaacacgccg agcatcagcg
     7981 tctcgagcac gaggaaggcg atcatgaact ccttcacacg ctcggtaatc gggacccagc
     8041 tggccaagat gcagagcggc atgagcaagg tggtgagcag cacgaacggc agggaaatgc
     8101 cgtcgatgcc catatggaag ctgatcggcc cgagccaggg ccgaagctcg acgaactgga
     8161 actgggcggt gagcggatcg aagtcgcgcc acaggatcaa cgacagcgcg aaggtaacga
     8221 gcgtggtcca cagcgcgatg aagcgcgcgt tgcgggacgc cgcttcgtcg ttcccacgca
     8281 aggcgacgat cagcaacgcg ccaagcagcg gcaggaaggt gactgtggac aggatgggcc
     8341 aggag
//
LOCUS       DASUQP010000197         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_779717,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000197 DASUQP010000000
VERSION     DASUQP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779717"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1034)
                     /gene="dusA"
                     /locus_tag="VFY74_08800"
     CDS             complement(27..1034)
                     /gene="dusA"
                     /locus_tag="VFY74_08800"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX5931829.1"
                     /translation="MKSDSHRFCVAPMMEWTDRHCRYFLRLISRRTFLYTEMVTAEAV
                     LFGNRERVLGFSPEEHPVGLQLGGSDPKRLAEAAKIGAAYGYDEINLNIGCPSDRVQS
                     GRFGACLMAEPDLVAHCVAAMGAAAAIPITVKCRIGIDDQDAEESLDRFIDLVAEAGC
                     WTFIVHARKAWLGGLSPKENREVPPLDHDRVRRVKGRRPELEIVLNGGIESFAEARRQ
                     LPEVDGVMLGRAAYADPYLLAEVDRALFGAREVPPSRLEVLDRFVPYVEAELSRGSRL
                     NSMTRHILGLFHGQPRARAFRRHLAENAHRDGAGLDVLMAARGIAAGGWDEQANAVAA
                     E"
     gene            complement(1083..1400)
                     /locus_tag="VFY74_08805"
     CDS             complement(1083..1400)
                     /locus_tag="VFY74_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5931830.1"
                     /translation="MTRGYGQEDCPVARALDAIGEKWSLLVLRDLLRKGPLRFQQLEE
                     GLDGVAPNTLSARLKSLESAGVIGTRLYESHPPRYEYFLTDKGNGLGPVLKALYNWGE
                     RYR"
     gene            1509..2264
                     /locus_tag="VFY74_08810"
     CDS             1509..2264
                     /locus_tag="VFY74_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931831.1"
                     /translation="MTIPAYLSVYVLTGSVAIVAAILFGLNRALARADWSAEQRARVV
                     GGAALILVLWLAGAIALGAAGAFHASAGDVPTLQYGVFLPILVGALLIWRSPLVGRII
                     DAVPQQWLVSVQAYRALGVIFLILYAGGKLPGLFAWPAGVGDVLVGVLAPIIGLAYAR
                     DPQQNGDLVAGFNLFGIADLAIAISTGFLTAPSLVQPFAVEPPNELIGLYPLVLIPVF
                     LVPVSILLHLASLAKLSRTVTDVDRRRKPGVAV"
     gene            complement(2334..3191)
                     /locus_tag="VFY74_08815"
     CDS             complement(2334..3191)
                     /locus_tag="VFY74_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5931832.1"
                     /translation="MRQGRSALGIVFGFLLGATAGAGAGYYAGNYLLDRPVNWYAADI
                     SKLGAGPENDLIRYGRDLIVNTPRHIGKNATDPAMRYAGNDLACQNCHLNAGLQPFAA
                     PFVSTFATFPMMVDDQVLTLTDRINGCMRRSLNGKDLPSEGREMEAIVAYLKFVGKGS
                     PEGVRVPGMGLRPIENPTSPPDARRGEAVYAKLCVTCHKDDGQGEPKPSPGVGYSIPP
                     LWGEASFNAGAGMAKTAHAASYIRDNMPMGVDYRAPVLTVQEAWDVAAYIISKPHPPA
                     TAETISTLQ"
     gene            3283..>3437
                     /locus_tag="VFY74_08820"
     CDS             3283..>3437
                     /locus_tag="VFY74_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX5931833.1"
                     /translation="MNERALKDAHMATDPVCGMMVDPHAGKPTEAFRGSTYFFCSEGC
                     RKKFAADP"
BASE COUNT          586 a         1120 c         1068 g          663 t
ORIGIN      
        1 gttgcaagtc gggatgccgc tgagccctac tcggccgcca ccgcgttcgc ttgctcatcc
       61 caaccgccgg cagcgatgcc gcgggcggcc atcagcacgt cgagcccggc gccgtcgcga
      121 tgggcgttct cggcgagatg ccgcctgaag gcgcgtgctc gtggctggcc gtgaaacagt
      181 ccgaggatgt ggcgggtcat ggaattgagg cgcgaccccc gcgacagttc cgcctcgaca
      241 taaggcacga agcggtcgag cacctcgagc cgtgacggcg gcacctctcg cgcgccgaat
      301 agcgcgcgat cgacctcggc caggaggtag ggatcggcat aggccgcgcg gccgagcatg
      361 acgccgtcga cctcgggcag ttgccggcgc gcctcggcaa aggactcgat cccgccgttg
      421 agaacgatct caagctcggg ccggcgccct ttcacccgcc gcacgcgatc gtggtcgagc
      481 ggcggcacct cgcgattctc cttggggctc aaccccccga gccaggcttt gcgcgcatgg
      541 acgatgaagg tccagcaccc ggcctcggcc acaagatcga tgaagcggtc gaggctctcc
      601 tcggcgtcct gatcgtcgat gccgatgcgg catttcaccg tgatggggat cgctgccgcc
      661 gcgcccatcg ccgcaacgca gtgagcgaca agatcgggct ccgccatgag gcaggcgccg
      721 aaccgccccg actgcacgcg atcggacggg cagccgatat tgagattgat ctcgtcatag
      781 ccgtacgcgg cgccgatctt ggccgcctcg gcgaggcgct tgggatcgct gccgccgagc
      841 tgaagcccga ccggatgctc ttccggagag aagccgagca cgcgctcgcg attgccaaag
      901 agcacggcct ctgccgtcac catctcggta tagaggaagg tccgccgcga gatcagccgc
      961 aagaaatagc ggcagtggcg gtcggtccac tccatcatcg gggcaacgca gaatcgatgt
     1021 gagtcagatt tcaacgactt atgctctcga agcgagtcga attgcgaacc ggaagcgccc
     1081 tctcaccggt agcgctcgcc ccaattgtag agggctttca gcaccggtcc gagaccgttg
     1141 cccttgtcgg tgagaaaata ttcgtagcgc ggcggatggc tctcataaag ccgggtcccg
     1201 atcacgccgg cgctttccaa gctcttgagc cgtgccgaca gcgtgtttgg cgccacgccg
     1261 tcgagaccct cctccagctg ctgaaagcgc agcggacctt tgcggagaag atcgcgcaaa
     1321 accagcagcg accatttctc gccgatcgca tcgagcgccc gcgccaccgg acaatcttcc
     1381 tggccgtatc cgcgtgtcat caacgctaga tagccaagtc ccttgacgat ttcaagtcac
     1441 ttgctattga caagttactt gtgctattat agctatgggc cgtgaccctg accctcagga
     1501 ggtgtgtcat gaccattcca gcctaccttt ccgtttatgt cctgacgggc tcggtcgcca
     1561 tcgtcgccgc tatattgttt gggctaaatc gtgcgctggc ccgcgccgat tggtcggcgg
     1621 aacaacgggc ccgcgtcgta ggcggggctg cgctcattct cgtcctgtgg ctcgccggcg
     1681 ccatcgcgct cggcgcggcg ggcgcctttc acgcgagcgc gggcgatgtc cccacccttc
     1741 agtacggcgt ctttctaccg atcctggtcg gcgcactgct catctggcgg tcgccgctcg
     1801 tcggccgcat catcgacgcc gtgccgcagc aatggctggt cagcgtccag gcctatcgcg
     1861 cgctcggcgt gatctttctg atcctctatg cgggcggcaa gttgccgggt ctgtttgcat
     1921 ggcctgccgg tgtcggcgat gtcctcgtgg gcgtgcttgc gccgattatc gggctcgcct
     1981 atgcccgcga tccgcaacag aacggcgatc tggtcgccgg cttcaatcta tttggcatcg
     2041 ccgatcttgc gatcgccatt tcaaccggtt tcctgaccgc gccttcgctg gttcagccct
     2101 tcgcggtgga gccgcccaat gagttgattg gcctctaccc cctcgtgctc attccggttt
     2161 tcctcgtgcc agtgtcgata cttctgcatc ttgcttcgct ggcaaagctc agccggaccg
     2221 tcaccgacgt tgacagacgg cgcaagcccg gtgtcgccgt ctaaagctct gcgcgcctcg
     2281 ccaagggaaa cgatcggctc aaggttagag acgccacgcg gagccgctct gtgttattgc
     2341 agcgtggaga tggtctccgc tgtggccggc ggatgcggct ttgagatgat ataggccgcc
     2401 acgtcccacg cttcttgcac ggtgagcacc ggcgcgcggt aatcgacccc catcggcatg
     2461 ttgtcgcgga tataagaggc ggcatgagcg gtcttggcca tgccggcgcc ggcgttgaag
     2521 ctcgcttccc cccacaacgg cggaatggaa tagccaacgc cgggactggg tttcggctcg
     2581 ccctgcccgt cgtccttatg acaggtcacg cagagtttcg catagaccgc ctccccgcgc
     2641 cttgcatcag ggggcgaagt cgggttctcg atcggcctca ggcccatgcc gggaacgcgc
     2701 acgccctccg gcgacccttt gccgacgaat ttgaggtagg cgacgattgc ttccatctcg
     2761 cggccctcgc tcggcaagtc cttgccgttc aggctgcgcc gcatgcagcc attgatgcga
     2821 tcggtgagcg tgagcacctg gtcgtcgacc atcatcggga aggttgcaaa ggtggagacg
     2881 aaaggtgcgg cgaagggttg caatcccgcg ttgagatgac agttctggca ggcgagatcg
     2941 ttgccggcat agcgcatggc gggatcggtc gcgttcttgc cgatatggcg gggagtattg
     3001 acgatgagat ccctgccgta gcggatgaga tcgttctcgg ggcccgctcc aagcttggag
     3061 atgtcggcgg cgtaccaatt gaccggccgg tcgagcaggt agttgcctgc gtaatagccc
     3121 gctccggcgc ctgccgtggc tccgagcagg aacccgaaga caatccccaa ggccgaccgt
     3181 ccctgtcgca tcctctgtcc tccagcccca tattattagg gtttcaatga tagccacccc
     3241 ggcttgcagt tctgcaagca agttcagcaa tcaccccaaa ccatgaacga gagagccctg
     3301 aaagacgctc acatggcgac cgacccggtt tgcgggatga tggtggaccc gcacgcgggc
     3361 aagcccaccg aagcgttccg cggcagcacc tattttttct gctccgaggg ctgccgcaaa
     3421 aagtttgccg cggaccc
//
LOCUS       DASUQP010000198        11424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_643777,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000198 DASUQP010000000
VERSION     DASUQP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643777"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..724
                     /locus_tag="VFY74_08825"
     CDS             224..724
                     /locus_tag="VFY74_08825"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX5931834.1"
                     /translation="MAKHRILFVCLGNICRSPMAEGVFRRVAEEKGVLHLFDIDSAGL
                     GHWHIGQAPDARAQDAARNRGIDISGQSARQVKHTDFARFDLILAMDGDNYQELVQLA
                     PSDARHKLRRFLDFAPQAPAKDVPDPFFGGPEGFDHALDLIEQAARGLLAALLADAAA
                     PVKRGA"
     gene            complement(721..1257)
                     /gene="thpR"
                     /locus_tag="VFY74_08830"
     CDS             complement(721..1257)
                     /gene="thpR"
                     /locus_tag="VFY74_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5931835.1"
                     /translation="MPRLFSAIEIPKSVAERLTLLRAGLTGARWIDPENYHLTLRFIG
                     DVDGHTARDFTAALGEIVAPPFELQLNGLGSFGGGKPRAIFAGIAPSEGLETLRRANE
                     RAAREAGLPPEGRNYKPHVTLARLRGARADAVAAYLERQGGIGPEPFTVDRFVLYSSR
                     NSVGGGPYVVEAAYPLES"
     gene            complement(1294..2334)
                     /locus_tag="VFY74_08835"
     CDS             complement(1294..2334)
                     /locus_tag="VFY74_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5931836.1"
                     /translation="MDHPELGRTGVRISRLCLGTRTFGEQNSEAEGHAQMDYAFGRGI
                     NIFDAAEIYRVPPKPETQGGTEAIIGTWLAARKVRDKVMIATKIAGRRKMTWLRRNET
                     ATRQSKAQITEAVEASLKRLKTDYIDLYQLHWPDRPMRIFEGLDYLHLEGDSHPVHDI
                     LGALGKLVAGGKVRFVGLSNETPWGVMSFLKTSDQHGLPRPVSIQNAYNLVNRSFETG
                     LSEVAYREQVGLLAYSPLGQGYLSGKYEGGALPPGSRKALFARLGRYEKGNGPKAISA
                     YIEVARRHGLDPAQMAIAFAVSRPFVTSTIIGATTMDQLRTDIAAAEVKLSDAVLADI
                     EQVHLDYPNPCP"
     gene            complement(2365..2526)
                     /locus_tag="VFY74_08840"
     CDS             complement(2365..2526)
                     /locus_tag="VFY74_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931837.1"
                     /translation="MPLNFGKFGAAIALLTVAAFVLWLIFTTFFATEQAEQQIERTGA
                     VALGASDAS"
     gene            2645..4033
                     /locus_tag="VFY74_08845"
     CDS             2645..4033
                     /locus_tag="VFY74_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5931838.1"
                     /translation="MARTDGGIGEAGRREPVQGDRAILVVCPTHRDHRELPRVSPPGV
                     KYLFHDYASTSLEDLICNVTRHGDFTAGPLEEMDAILAKVAGIRLAGVISTDDYPGSA
                     LAAAIAKRLGLPGPDPADTLICQHKYLSRIEQAKRVPHAVPPFALIDVAPGVPLPEGL
                     RFPLFVKPVKSFFSIGAERIDSASALAALFPRWAALDQFFAPLDQMLQRYTGVKIGTK
                     RLIAEGLLKGEQVTVEGFAHEGEARIMGVVDSVMFPGTLAFARFDYPSALPESVQRRM
                     ADIAATVMTGLGFDNGMFNIEMMYDREADLISIIEINPRMASQFADLYEKVDGTNSYA
                     VLLDIAQGRTPSFARRRGRYAFASSIVLRSFEDFFVAALPSETDLDRLARDDPDIRTE
                     ILVRVGRTLSSELQDGNSYRYGIVNLGGRNRADVLARFAACRERLGIVLLPIEQPEPA
                     GSVTPEIARVDA"
     gene            4150..5979
                     /gene="typA"
                     /locus_tag="VFY74_08850"
     CDS             4150..5979
                     /gene="typA"
                     /locus_tag="VFY74_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5931839.1"
                     /translation="MNLRNIAIIAHVDHGKTTLVDRLLQQSGAFRDNQRVAERALDSN
                     DLERERGITILAKVTSILWQGTRINIVDTPGHADFGGEVERILNMVDGVLVLVDAAEG
                     PLPQTKFVVSKALKMGLKPIVVINKVDRADARPQVVVDEVFDLFAALDASDEQLDFPI
                     LYGSAKEGWMAAAPEGPKTLGMQLLFDLVLTHVAPPKIEDGPFRLLGTILEANPYLGR
                     IVTGRITSGSIAPNEQVRVLDHAGNVVEQGRVTKVLAFRGLERVGLDEAGAGDIVSLA
                     GLPNATVAHTIAAPEVIAPLPAQPIDPPTLSMTFRVNDSPLAGTEGTKVTGRMIRDRL
                     LREAEGNVALRVTESEERDSMEVAGRGELQLGILIETMRREGFELSVSRPRVLFRRGP
                     DGTLLEPIEEVVIDLDEEHSGIVVRKLSERKAELVEMKPSGGGRLRLRFFAPTRGLIG
                     YQGELLTDTRGSGIINRLFHAYAPHKGPIQGRRNGVLISTESGDAVAYALWNLEDRGA
                     MMIDPGTRVYGGMIVGEHTRDNDLGVNVLKGKKLTNIRTTSKDEAVRLTPPLRMTLER
                     ALAYITDDELVEVTPKSIRLRKCHLDPNERKKAERQRLAEDVA"
     gene            6063..6566
                     /locus_tag="VFY74_08855"
     CDS             6063..6566
                     /locus_tag="VFY74_08855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5931840.1"
                     /translation="MTVDVVQIRPPRFKDAPALAAAYEEAWRNAYQGIIPHLSLERMI
                     ARRGLRWWESALRKRTPLLVLDFGGEAAGYVTYGRSRLRTQFQGEIFELYLHPIYQGL
                     GFGEKLFDGARSRLSELRLRGLLVWALADNDAACAFYLSLGGKPVAEGAESFGDVSLR
                     KLAFAWA"
     gene            6756..7562
                     /gene="dapB"
                     /locus_tag="VFY74_08860"
     CDS             6756..7562
                     /gene="dapB"
                     /locus_tag="VFY74_08860"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5931841.1"
                     /translation="MSDMRIAVLGAAGRMGQALTRVLVETPGCAVAGGIEAKGSPLVG
                     RDLGEVAGLDPLGIAITDDPLPVFAHADGVLDFTTPPATVAFSALSAQARIVHVIGTT
                     GLSQADDAKIEAAARHASIVKAGNMSQGVNLLAALAARVASALGPEFDIEILEMHHRH
                     KRDAPSGTSLLLGKAAAEARGISLAERGVRTRDGDVGPRREGDIGFAALRGGDVVGEH
                     RVIFAGPGERIELAHIATDRGIFARGAVKAALWARGRGPGLYAMTDVLGL"
     gene            7720..8376
                     /locus_tag="VFY74_08865"
     CDS             7720..8376
                     /locus_tag="VFY74_08865"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX5931842.1"
                     /translation="MTERPDSADTPPGENVLVLVRHGESEWNRANRFTGWKDVGLTEE
                     GMAEAHRAGAMLKKSGVRFDCAFTSTLKRAHNTLDIILEELGQRKLPTVKAAALNERD
                     YGELVGINKEEARKRWGSEQVHIWQRSYDTAPPGGESLKDTALRVLPFYEKWIMPELQ
                     KGKSVILVAHGNSLRSLIMELDRLSPDEVMQVELGTAMPLVYRLNADGTVAEKRDLAA
                     "
     gene            complement(8398..8850)
                     /locus_tag="VFY74_08870"
     CDS             complement(8398..8850)
                     /locus_tag="VFY74_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX5931843.1"
                     /translation="MLTPHRSLGPKGFMVLMAAVCVVSFATGLFFFLLGAWPVIGFMG
                     LDVALIYVAFRLNFRALKLYETVDLTQDALTVTRVAPSGKSQSWSFNPYWVRLSLQPR
                     LGRSSELSLASHGKRLVFASFLSDAEREDFASALSSALASARSAGAVY"
     gene            8958..9662
                     /gene="nth"
                     /locus_tag="VFY74_08875"
     CDS             8958..9662
                     /gene="nth"
                     /locus_tag="VFY74_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5931844.1"
                     /translation="MAERRTAGKTRVPAKRARGSRLSREAIDEIFRRFAKLDPDPNTE
                     LHYRDPFTLLVAVVLSAQATDASVNRATPDLFHIADTPEKLAGIAVAELEAKIKTIGL
                     YRSKAKNLVALAKKLVAEHGGKVPEAREVLMALPGVGRKTANVVLNTAFGQNTIGIDT
                     HAFRVANRTGLAPGKTPLEVELKLERLVPERYKRNCHHWLILHGRYVCKARRPECWRC
                     LIADLCLYKPKTPAPA"
     gene            complement(9678..10364)
                     /locus_tag="VFY74_08880"
     CDS             complement(9678..10364)
                     /locus_tag="VFY74_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter family protein"
                     /protein_id="HEX5931845.1"
                     /translation="MLSAAGQAFNDVFTPPFRAVLFKCVAFTIGLLVLLIVGIEWTFS
                     HFVQWPGWIETSIQWLGGLALVVGSIFLIPPVTSLIAGLYLDDIAAVVEREHYPADPP
                     GEALPTLQAIGVALRFFFVVLLVSLVALFLLLIPGVNLIAFYLGNGYLLGREYFELAA
                     MRHVPPAEAKALRKANRLTVLLSGLVIAGIASVPILNLITPLFATGFMVRVYKALARS
                     AGLRPSHERP"
     gene            10483..>11424
                     /locus_tag="VFY74_08885"
     CDS             10483..>11424
                     /locus_tag="VFY74_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX5931846.1"
                     /translation="MAGKTYDVVGIGNAIVDIIARCDEGFLTKHDLVKGSMRLIDAEE
                     ANRLYSAMGPAVERSGGSVANAIAGLASFGTSCAFIGRVAADQFGGIFRHDIRSLGVA
                     YDTLPATDGAPTARCLILVTPDGERTMNTFLGASVDFAPRDLDLPMIAAAKIVYLEGY
                     LFDRAEAKAAFRKAARAARAAGAKVALSLSDAFCVDRHRKDFRALVRDRADIVFANEK
                     EITTLYRVNSFEEAARAALADCEMAVLTRSEAGSVIVAGGESLKIEAEKVARVVDLTG
                     AGDLYSAGFLYGLTQGAPLAVCGRLGSLAAAEVIGHIG"
BASE COUNT         2054 a         3731 c         3703 g         1936 t
ORIGIN      
        1 cttctcggca aaagcggcga gcgaaatctg ccggccaaga gccgcgtgct tgagctcgct
       61 tgcttcgttc caggcaacca tcgttctcac ctcgcagaaa gagataagcc gaatccctca
      121 agtgaaaata ggatatgggc agaggccctg caaccgcggt cctcgcgctg ggaaatcgcc
      181 gcccggcttg cacggcaacc ggccgcatgc catttgaccc ggcatggcaa agcaccggat
      241 cctgttcgtc tgcctcggca atatctgccg ctcgccgatg gctgaaggcg tcttccgccg
      301 cgtcgccgag gagaagggtg tgctccatct cttcgacatc gactctgccg gactcggtca
      361 ctggcatatc ggccaggcac cggacgcccg cgcgcaagat gcggcgcgca atcgcggcat
      421 cgacatctcg ggccaaagcg cgcggcaagt gaagcatacc gatttcgccc gcttcgacct
      481 catcctcgcc atggacggag ataattacca ggagctggtt cagctcgcgc cgagcgacgc
      541 gcgacacaag ctccgccgct ttctcgattt cgcgccgcag gcgccggcca aggacgtgcc
      601 cgatccgttc tttggcggcc cggaagggtt cgaccacgcg ctcgatctga tcgagcaggc
      661 cgcacgcggt cttcttgcgg ccctcctcgc cgatgcggcg gcacctgtca aacgaggcgc
      721 ctagctctcg agcggatagg ccgcttcgac gacatagggt ccgccgccga cagagttgcg
      781 cgaggagtag agcacgaagc ggtctaccgt gaacggctcc ggtccgatgc ccccctggcg
      841 ttcgagatag gcggcaaccg cgtcggcccg cgcgcctcgg agccgcgcca gcgtcacgtg
      901 cggcttgtag ttgcggccct ccggcggcag gcccgcttcg cgtgccgccc gttcattcgc
      961 ccggcgcaac gtttcgagcc cttccgacgg cgcgatgccc gcgaagatcg cgcgcggctt
     1021 gccgccgccg aaactgccaa gaccgttcag ttgaagctca aacggcggcg ccacgatctc
     1081 gccgagcgcg gcggtgaagt cccgcgccgt gtgtccgtcc acgtcgccga tgaagcgcaa
     1141 cgtcagatga tagttctcgg ggtcgatcca gcgcgcgccc gtaagccccg cgcggagcag
     1201 cgtcagccgc tcggcgacgc tcttgggaat ctcgatggcg gaaaagagtc ttggcatcgg
     1261 ctagggagct ttcgtcacgc ggcaggcaaa gcgtcaggga caggggttgg gatagtcaag
     1321 atggacttgc tcgatatccg caagcacggc gtcggacaat ttgacttccg ccgcggcgat
     1381 atcggtcctg agctggtcca tggtggtggc accgatgatg gtgctggtga cgaacggccg
     1441 cgacaccgca aacgcgatcg ccatctgcgc cgggtcgagc ccgtgtcggc gcgcaacttc
     1501 tatataggcg gaaatcgcct tgggaccatt ccctttctcg taacggccga gccgcgcgaa
     1561 cagcgccttg cgcgaacccg gcggcagcgc gccgccttcg tacttgccgc tcagatagcc
     1621 ctggcccaga ggcgaatagg cgagcaggcc gacctgctcg cggtaagcga cttccgagag
     1681 acccgtctcg aaggagcggt tgacgagatt gtaggcgttc tggatcgaga ccggccgggg
     1741 cagcccatgc tggtcggagg ttttcaggaa gctcatgacc ccccatggcg tctcgttcga
     1801 caggccgacg aacctcacct ttccgcccgc cacgagcttt ccgagagcgc cgaggatgtc
     1861 gtggacggga tggctgtcgc cttcgagatg caggtagtcg aggccctcga agatgcgcat
     1921 cgggcggtcg ggccagtgga gctgatagag gtcgatataa tcggtcttga ggcgcttcag
     1981 gctcgcctcg acggcctcgg tgatctgcgc cttgctttgc cgcgtcgcgg tctcgtttct
     2041 gcgcagccag gtcatcttgc gccggccggc gatcttggtc gcgatcatca ccttgtcgcg
     2101 gaccttccgc gcggcaagcc atgtgccaat gatcgcctcg gtaccgcctt gcgtctcggg
     2161 cttgggcggg acacggtaga tctcggcggc atcgaagatg ttgatgccgc ggccgaaggc
     2221 gtaatccatt tgcgcatggc cctcggcttc gctgttctgc tcgccgaagg tcctggtgcc
     2281 gagacagagc ctcgaaatcc tcacgccggt gcggccaagc tcggggtgat ccatggcgat
     2341 gcgtggggac ttaaggggcg cggcctagct cgcgtcgctc gcgccgagcg ccacggctcc
     2401 ggtccgctcg atctgctgct cggcctgctc ggtggcaaag aacgtggtaa agatcagcca
     2461 cagcacgaag gccgcaacag tcaggagcgc gatggccgcg ccgaatttgc cgaagttcag
     2521 gggcatgggt caggtaacgc ggttgaagcg cggcgacact agccaagtcg gacgccgcga
     2581 tcaatcgagc tgctctcgcc cgaaaggaat ccagccgacc cggccagggg caaaggacgc
     2641 ttcgatggca agaactgacg gcgggatcgg agaggccggg aggagggaac cggtgcaagg
     2701 cgatcgcgcg atcctcgtcg tctgccccac ccatcgcgac catcgcgagc ttccgcgcgt
     2761 ctccccaccc ggtgtcaagt acctgtttca cgactacgcc agcacgagcc tcgaagacct
     2821 gatctgcaat gtcacgcgcc atggggactt caccgcaggc ccactcgaag aaatggacgc
     2881 gatcctcgcg aaggtcgcgg gcattcgtct cgccggcgtg atcagcaccg acgattaccc
     2941 tggctcggcc ctcgccgcag ccatcgccaa gcggctcggt ctccctggac ccgatccggc
     3001 ggatacactg atttgccagc acaaatactt gtcgcggatc gagcaagcca aacgcgtgcc
     3061 gcatgcggtg ccgccgtttg cgctcatcga tgtggcgccc ggcgtgcccc ttcccgaagg
     3121 tttgcgcttc cccctcttcg tcaagccggt gaagtcgttc ttctcgatcg gagccgagcg
     3181 catcgactcg gcctcagcgc tcgccgcgct cttcccgcgc tgggcggcgc tcgatcaatt
     3241 cttcgcgccc ctcgaccaga tgcttcagcg ctacaccggc gtgaagatcg gcacgaagcg
     3301 gctgatcgcc gaggggctgc tgaagggcga gcaggtgacc gtcgaaggtt tcgcgcacga
     3361 aggcgaggcc aggatcatgg gcgtggtcga ttccgtgatg ttccccggca cgctcgcctt
     3421 cgcgcgcttc gactaccctt ccgccctacc tgagagcgtg cagcggcgca tggccgacat
     3481 tgccgcgacc gtcatgacgg ggctcggctt cgacaacggc atgttcaata tcgagatgat
     3541 gtacgaccgc gaggcggatc tgatctcgat catcgagatc aatccgcgca tggcgtcgca
     3601 attcgccgat ctctacgaga aggtcgacgg cacgaacagc tatgcggtcc tgctcgacat
     3661 cgctcaaggc cgcacgccca gcttcgcgcg gcggcgggga cgctacgcgt ttgcctcgag
     3721 catcgtgctg cgcagcttcg aggacttttt cgtcgcggcg ctgccaagcg agaccgatct
     3781 cgaccgattg gcgcgcgacg accccgacat caggaccgag attctcgtga gggtgggacg
     3841 tacgctttca tctgaattgc aggatggaaa cagctatcgc tacggcatcg tcaatctcgg
     3901 cgggcgcaac cgtgccgatg tgctcgcccg attcgccgcg tgccgggaga ggctcggtat
     3961 cgtgcttctg ccgatcgagc agcccgagcc ggccggctct gtgacgccag aaatcgcccg
     4021 cgtcgatgcc taaagcaagg cggtagcctc ggtttgcacc ttcagccgtt gcgcggccgc
     4081 ccggagaact atatatccgg cgcatgaaac gcggaccgga acggcccgcg ttctttcgtt
     4141 ttgggtcaaa tgaacctccg caatatcgcc attatcgccc atgtcgatca tgggaagacg
     4201 accctggtcg accggctcct gcagcaatcc ggcgccttcc gcgacaacca gcgggtggcc
     4261 gagcgcgcgc tcgactccaa cgatctcgaa cgcgagcgcg gcatcaccat tctcgccaag
     4321 gtcacctcga tcctgtggca aggcacccgc atcaatatcg tggatacgcc gggccacgcc
     4381 gatttcggcg gcgaggtcga gcgcattctc aacatggtcg atggcgtcct cgtcctggtc
     4441 gacgccgccg aaggtcccct gccacaaacc aagttcgtcg tgtccaaggc gctcaagatg
     4501 ggtctgaagc ccatcgtcgt catcaacaag gtagaccgcg cggacgcacg cccgcaggtg
     4561 gtggtggacg aggtgttcga cctgttcgcc gcactcgatg cgagcgacga gcagctcgac
     4621 tttcctatcc tttacggctc cgccaaggag ggctggatgg cggccgcgcc cgagggaccg
     4681 aagacgctcg gcatgcagct cctgttcgat ctcgtgctca cgcatgtcgc gccgccaaag
     4741 atcgaagacg gcccgttccg cctgctcggc accatcctcg aggcaaaccc ctatctcggc
     4801 cgcatcgtca ccggccgcat cacgtccggc tccattgcgc cgaacgagca ggtcagggtg
     4861 ctcgaccatg ccggcaatgt cgtggaacag ggccgggtca cgaaggtgct cgcctttcgc
     4921 ggtcttgagc gtgtcggtct cgacgaggcg ggcgcgggcg acattgtttc actcgctggc
     4981 ctgcccaacg ccacggtcgc ccatacgatt gccgcgcccg aggtgatcgc gccgcttccc
     5041 gcccagccga tcgatccgcc cactctgtca atgaccttcc gggtcaacga ttcgccgctt
     5101 gccggcaccg aggggaccaa ggttacgggg cgcatgatcc gggaccggct gctgcgcgag
     5161 gccgagggca atgtcgcact gcgcgtcacc gaatcggagg agcgcgactc catggaggtc
     5221 gccggtcgcg gcgagcttca gctcggcatt ctcatcgaga ccatgcgccg cgagggcttc
     5281 gagctttcgg tgtcgcgccc cagggtcctg ttcaggcgcg gccccgatgg cacgctgctc
     5341 gaacccatcg aggaggtcgt gatcgatctc gacgaggagc attccggtat cgtcgtccgc
     5401 aagctctcgg agcggaaggc cgagctcgtc gagatgaagc cctccggcgg cggccgcttg
     5461 cgccttcgct tcttcgcgcc cacccgggga ctgatcggct accagggcga gcttctcacc
     5521 gacacccgcg ggtctggcat catcaaccgg ctgtttcacg cctacgcgcc gcacaagggg
     5581 ccgatacagg gtcggcgcaa cggcgtgctg atctcgaccg aaagcggcga cgccgtggcc
     5641 tacgccttgt ggaacctcga agatcgcggc gccatgatga tcgaccctgg aacgcgggtc
     5701 tatggcggca tgatcgtggg cgagcacacg cgcgacaacg atctcggagt caacgtgctc
     5761 aagggcaaga agctcaccaa tattcgcacg acctcgaagg acgaagcggt acgtcttacg
     5821 cctccgctcc gcatgacgct cgagcgggcg cttgcctata tcaccgacga cgagctggtg
     5881 gaggtcacgc cgaaatccat ccgtctgcgc aagtgccacc tcgatccgaa cgagcgcaag
     5941 aaggcagagc ggcagcgtct tgccgaggat gttgcctgat cgtcacgcgt caagagatag
     6001 ctggcgggaa aatttgcaac gacccctttg ccgagcgccg ccctcctgcg ctaggcttaa
     6061 gcatgaccgt cgatgtcgtg cagatcaggc cgccgcggtt caaggatgcg cccgcccttg
     6121 ccgccgccta cgaagaggcc tggcgcaacg cttatcaggg catcatcccg catctttcgc
     6181 tcgagcgcat gatcgcgcgg cgcgggcttc gctggtggga gagcgcgctc cgcaagcgga
     6241 cgccgcttct cgtgctcgac ttcggcggcg aagcggcggg ctacgtgacg tatggccgct
     6301 cgaggctccg cacgcagttt caaggcgaga tcttcgagct ttacttgcac ccgatctatc
     6361 aaggcttggg gtttggcgag aagcttttcg atggcgcccg cagccggctt tccgaactca
     6421 ggctgagggg cctgctggtt tgggcgctcg ccgacaacga cgcggcgtgc gccttctacc
     6481 ttagcctggg cggcaagccg gtcgccgagg gcgccgagag cttcggcgat gtctcgttgc
     6541 gcaaattggc cttcgcctgg gcttaagcct cgagtttcac cccgggtcct tgccccggaa
     6601 ccccctccta ggggactaac gacgcgtcag gcctaatcca agcgaggggc ttgtgggcga
     6661 ggtctctgcc gtcatagaag gtcggcgcgt gggggattcg agggaacaag ccgggagtcc
     6721 gtcggcgccc cgcacggcct gatggtcaac ggccaatgag tgacatgaga atcgccgtgc
     6781 tgggggcggc cggccgcatg ggccaggcgt tgacgcgggt gctcgtcgag acgccaggat
     6841 gcgccgtggc cgggggaatc gaggccaagg gctccccgct tgtcggccgc gacctcggcg
     6901 aggtggcggg cctcgacccg ctcggcatcg ccattaccga cgatcccctc cccgtctttg
     6961 cccatgccga cggcgtgctc gacttcacca ccccgcccgc gaccgtcgcc ttctccgcgc
     7021 tttcggcgca ggcccgcatc gtgcacgtca tcggcacgac cggcctctct caggctgacg
     7081 acgccaagat cgaggccgcc gcgaggcacg cctccatcgt caaggcgggc aatatgagcc
     7141 agggcgtgaa ccttctcgcc gctctcgccg cgcgggtggc ctcagccctc ggccctgagt
     7201 tcgacatcga gattctggag atgcaccacc gccacaagcg cgatgcgccg tcgggcactt
     7261 ctctcctgct cggcaaggcc gcggccgagg cgagaggcat ctcgcttgcc gagcgcggcg
     7321 tgcggacccg cgacggcgat gtcgggccgc ggcgcgaagg cgatatcggc ttcgcggcgc
     7381 tccgcggcgg cgacgtggtt ggcgagcatc gggtgatttt cgccgggccg ggcgagcgga
     7441 tcgagcttgc ccatatcgcc accgaccgcg gtattttcgc ccgcggcgcg gtcaaggcgg
     7501 cgttatgggc gcgcggacgc ggccccgggc tttatgcgat gacggacgtg cttggcttgt
     7561 gagttctttt aggtgcgaag atttaggtgc gaagatttgg catctggcga aacgatgtac
     7621 accgacctaa aaggcgtcaa tggtggcttc gcaaattgcg atcgaactta aggttctctc
     7681 gttaggcgtt ctgtgcgaca ggaagcatcg gctgaggcaa tgaccgaacg ccccgacagc
     7741 gcagacaccc ctcccggcga aaacgtgctc gttctcgtcc gccacggcga gagcgagtgg
     7801 aaccgggcga accgcttcac cggttggaag gacgtcggcc tcaccgaaga gggcatggcc
     7861 gaggcccacc gcgccggcgc gatgctgaaa aagagcggag tgcgtttcga ctgcgccttc
     7921 acctcgacgc ttaagcgcgc acataacacg ctcgacatca tcctcgagga gcttggccag
     7981 cgcaaacttc caaccgtcaa ggccgccgcg ctgaacgaac gcgactatgg cgagcttgtc
     8041 ggcatcaaca aggaggaggc gcgcaagcgc tggggatcgg agcaggtcca catatggcag
     8101 cgctcctacg acaccgcccc tcccggcggc gagtcgctca aggataccgc gttgcgcgtc
     8161 ctgccgttct acgagaaatg gatcatgcca gagctgcaga agggcaagag tgtgatcctg
     8221 gtcgcccacg gcaattcgct ccgctccttg atcatggagc tcgaccgcct atcgccagat
     8281 gaagtgatgc aggtcgagct cggcaccgcc atgcctctgg tctaccggct caatgccgac
     8341 ggcaccgtcg ccgagaagcg cgatctcgcc gcctagtcat tacgcttgct gtgacgctta
     8401 atacactgcg ccggcgcttc gcgccgaagc gagcgccgag gatagcgcgg aggcgaagtc
     8461 ctcacgctcc gcgtcggaga ggaagctcgc gaatacgagc cgcttgccgt gcgaggcgag
     8521 cgacagctcg ctcgaccgtc cgagccgcgg ctggaggctc agtctgaccc aatagggatt
     8581 gaagctccac gattgcgatt tcccggaggg cgcaacgcga gtgaccgtca gcgcgtcttg
     8641 ggtcagatcg acagtttcgt agagcttgag cgcgcggaag ttgagccgga acgccacata
     8701 gatgagggcg acgtcgagcc ccatgaagcc gatcaccggc caggcgccga gcaggaaaaa
     8761 gaacagtccg gtggcgaagc tcacgacgca caccgccgcc atgagcacca tgaagccctt
     8821 gggtccgagg gagcgatgcg gcgtgagcac ggcgctgaac cgcgaaggcc tcccatttga
     8881 agggtcttgt tgtgcggcgt ttgcctctgt catagcaagg aaactgctaa tcccctggcg
     8941 agcgtgaggc aaggcccgtg gcggaacgaa ggacggccgg caagacgcgg gtcccggcca
     9001 agcgggcgcg cggatcgcgg ctcagccgcg aggcgatcga cgagatcttc cgccgcttcg
     9061 caaagcttga ccctgatcca aataccgagc tccattaccg cgaccccttc acgctgctcg
     9121 tggccgtggt cttgtcggcg caagcgaccg acgcctccgt caaccgcgcg acgcccgatc
     9181 tgttccacat cgccgatacg ccggaaaaac ttgccggtat cgccgtggcc gaactcgagg
     9241 ccaagatcaa gacgatcggc ctctaccgca gcaaggcgaa gaacctcgtt gcgctcgcaa
     9301 agaaacttgt tgccgaacat ggcggcaagg tgcccgaagc gcgcgaggta ctgatggcgc
     9361 ttcctggcgt cgggcgcaag accgccaatg tcgtgctcaa caccgccttc ggccagaaca
     9421 cgatcgggat tgatacccat gctttcaggg tggccaaccg gacgggcctc gcgccgggca
     9481 agaccccgct cgaagtcgag ctcaagctcg agcgcctcgt gccggagcgc tacaagcgca
     9541 actgccacca ctggctgatc ctgcacgggc gctatgtgtg caaggcgagg cggcccgaat
     9601 gctggcgctg cctcatcgcc gatctctgcc tctacaagcc caagacgccc gctcccgcgt
     9661 aaccggcccg acttctgtca aggtcgctcg tgcgaaggac gaagccccgc gcttcgcgcc
     9721 agcgccttat acacgcgcac catgaagccg gtcgcgaaga gcggcgtgat caggttgagg
     9781 atcggcaccg aggcgatgcc ggcgatgacg agaccgctca acagcacggt gaggcgattg
     9841 gctttcctga gcgccttcgc ctctgcgggc ggaacatgcc gcatcgccgc aagctcgaaa
     9901 tattcgcgcc ccagcaggta gccgttgccg agatagaagg cgatcagatt gacgccgggg
     9961 atgagcagaa ggaacagcgc gaccaggctc accagcagca cgacgaagaa gaaccgcagc
    10021 gccacgccga tggcctgcaa tgtcggcagt gcctcgcccg gaggatcggc gggataatgc
    10081 tcgcgctcga ccaccgcggc gatgtcgtcg agatagagtc ccgcgatcag cgatgtgacc
    10141 ggcgggatga ggaagatcga accgaccacg agcgcgagcc ccccgagcca ctggatcgat
    10201 gtctcgatcc agccgggcca ctgcacgaaa tggctgaagg tccactcgat gccgacgata
    10261 agaaggacga ggagcccgat ggtgaaggcc acgcatttga acagcacggc gcggaagggc
    10321 ggcgtgaaca cgtcgttgaa ggcttgacct gcggcgctga gcatgacgtg ttcctttccg
    10381 gcgacggaat ataatgggcg gagccgaaga ctccagggtg gaacagacac cgggggtaga
    10441 agagacaccg cgggcgaaga gcaaggcggg gcggtcaagc acatggcagg gaagacttac
    10501 gacgtggtcg ggatcggcaa cgccatcgtc gacatcatcg cgcgctgcga tgagggcttc
    10561 ctgaccaagc acgacctcgt caagggctcg atgcgcctga tcgatgccga ggaggcgaac
    10621 cgcctttatt cggcgatggg gcccgcggtc gagcgctcgg gcggatcggt ggcgaatgcg
    10681 attgccggtc ttgcctcgtt cggcaccagt tgcgccttta tcggcagggt ggcggccgac
    10741 cagttcggcg ggatcttccg ccatgacatc cgctcgctgg gggtggccta cgacacgctc
    10801 cccgcgaccg acggggcgcc gaccgcgcgc tgcctcatcc tcgtgacgcc cgacggcgag
    10861 cgcaccatga acacctttct cggcgcgagc gtcgattttg cgccccgcga tctcgatctt
    10921 ccgatgatcg ccgccgccaa gatcgtctat ctcgaaggct atctgttcga ccgcgccgag
    10981 gccaaggctg ccttccgcaa ggccgcccgc gcggcgagag cggcaggggc caaggtggcg
    11041 ctcagcctgt cggatgcctt ctgcgtcgac cgccaccgca aggatttcag agccctggtg
    11101 cgagaccgcg ccgacatcgt cttcgccaat gagaaggaga ttacgacgct ctaccgggtc
    11161 aacagtttcg aggaggcggc ccgggctgct ctcgccgact gcgagatggc cgtactgacc
    11221 cgttcggaag cgggctcggt gattgtggca ggaggcgaga gccttaaaat cgaggccgag
    11281 aaggtcgctc gcgtggtcga tttgaccggg gccggagacc tctactccgc cggctttctc
    11341 tacgggctga cgcaaggggc gccactggcc gtttgcggcc gcctggggag cctcgccgcc
    11401 gccgaggtga tcggccatat cggc
//
LOCUS       DASUQP010000199         9651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_508632,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000199 DASUQP010000000
VERSION     DASUQP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508632"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..984
                     /locus_tag="VFY74_08890"
     CDS             19..984
                     /locus_tag="VFY74_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5931847.1"
                     /translation="MRAKMISVGLLGAAALVPVDFAKADQLGHASWYSLPANKTANGE
                     RMNPDELTAAHRSLPFGTRVVVENLSNGRSVVVRINDRGPFIGGRIIDLSKAAAASIG
                     MIATGTAKVRVTTAAGGVLAGLGESTVKAKGESRIHEAASPAAPEAAVQIVSAKMTGA
                     TATEAKASPKPSQQANVASKPAKTKTLAVTASAASKKRVGSQTIKVAAAKRSRPSANA
                     KARVQVVAAANGLGTGREASRASSRRVVVASNGIRAAAREASRFSSRRMIVASNGIRT
                     AAREASRFSSRRVNVASNTLNPYAVSGTTPGFTKSARVRARRAMG"
     gene            1579..1890
                     /locus_tag="VFY74_08895"
     CDS             1579..1890
                     /locus_tag="VFY74_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931848.1"
                     /translation="MFKLPASRKLILTAVAGAFLALVAIEDASAQQAASGNQSAQGAG
                     DYPRSYGPVRPRPGGFSRLTLSDIWGPAKMPPPPIDFGPHFDFPPAPLNGAPLHDPYP
                     N"
     gene            2125..2340
                     /locus_tag="VFY74_08900"
     CDS             2125..2340
                     /locus_tag="VFY74_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5931849.1"
                     /translation="MRQTGTVKFFNHSRGFGFITPDDGGKDVFLHISALQRSGLPALD
                     EGARVSFETEPDRRGKGPQAINVQMGE"
     gene            2471..3892
                     /locus_tag="VFY74_08905"
     CDS             2471..3892
                     /locus_tag="VFY74_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5931850.1"
                     /translation="MNESEAPVSALQSVLGRLAREEPRALERLFGLLAIPSVSTDPAY
                     HQACVDAAECCAGLLREAGFDARVVPTSGKPMVVGHSRARPSAKPRRHALFYGHYDVQ
                     PPEPFEAWTVPPFQPHLADDPHHGKVIVARGASDDKGQMMTFIEAARAWRDELGTLPL
                     DVSVLIEGEEESGSPSLAPFLAAHGDELKADLALVCDTAQWDAETPAITAFLRGLAFS
                     EITVRGPSRDLHSGVYGGPALNPLRVLSEILAALHDGEGRVAIPGFYDGIRAPSPAEL
                     ASWRALGFDEAGFLGDVGLREPAGEKGVSMLEQLWSRPTAEINGIVGGYGGPGTKTVI
                     PAQATAKLSFRLVPGQDPAHVIACFHRFVSARLPADAKVSYAHEGGSPAIGFDTSAPA
                     FVAAARALEAEWGKAPAIIGCGASIPIVESFRSRLGMDSLLVGFALDDDRIHAPNEKY
                     NLQSFTKGMRSWVRILAELGALA"
     gene            3892..4578
                     /locus_tag="VFY74_08910"
     CDS             3892..4578
                     /locus_tag="VFY74_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEX5931851.1"
                     /translation="MRSGLTRSLPLPLALALALVSLTGASGAAGAERNTPGAFDYYAL
                     VLSWIPSYCRGEGKGRKDKQCDGTKPHAFLLHGLWPQHEIGWPLDCRTVKRPWVPESV
                     IAEMGDIMPSKNLVIHEYRAHGTCSGLEPKEYFAAARALYERISVPAAFLAPGADLDS
                     PEEIERAFLDANPWLTPDMISITCRKAHLLDIRVCFGRDLSPRNCGVNEDQRRLCAAP
                     KVVVPPPAQR"
     gene            complement(4587..5237)
                     /locus_tag="VFY74_08915"
     CDS             complement(4587..5237)
                     /locus_tag="VFY74_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 12"
                     /protein_id="HEX5931852.1"
                     /translation="MLDENAVARAHAVYTPFALSIYDVLVHGLSNRFAWRCPTRRLVE
                     LYRDNLSPDHLEAGVGTGVLLDRAGTERLKRLVLLDANRNCLDRSALRLARFDLELHQ
                     VDLLQPVAFDTAPVASVGLTYVLHCLPGRMDEKLKAVDHLRPLMRDGATLFGATILGR
                     GIAPNRAASALLDLYNAQGVFNNREDDLASLSDGLRQRFADVAIAQEGCVAIFRAN"
     gene            5383..6144
                     /locus_tag="VFY74_08920"
     CDS             5383..6144
                     /locus_tag="VFY74_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5931853.1"
                     /translation="MAKDKHDRTVLVTGAARRLGRIIALDLGARGWRVGVHYRASAAE
                     AAALVAEIERNGGTAAALAADLDRLDALSKLIADCASSLGPPACLINNAARFEHDTLG
                     TLQGETWQAHLDVNLRAPIFLTQAFARTLPDGVEGNVINIIDQKVLRPDPDYFSYTMA
                     KAALWSATQIMAQALAPRIRVNAIAPGPVLNADGQSESAFEREYRETLLQRAVGTDDV
                     SRAVRFLLETPSITGQIIALDSGQHLVFKAKSGDH"
     gene            6240..8153
                     /gene="uvrC"
                     /locus_tag="VFY74_08925"
     CDS             6240..8153
                     /gene="uvrC"
                     /locus_tag="VFY74_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5931854.1"
                     /translation="MPLENPDPAGSTRLARALDALRAVETGAPVIGRHAKTAPAGPGV
                     YRMIDAAGEVLYVGKARHLKKRVQSYARGAGHNNRIARMVADTASMEFVTTATEIEAL
                     LLEANLIKRLKPRFNVVLRDDKSFPFILIARDHPVPQILKHRGARNRRGDYFGPFASA
                     GAVGRTINTLQRAFLLRSCSDAYYESRTRPCLLFQIKRCSAPCTGEISLADYGALVDE
                     ALRFLTGESDEVKLRLRALMDEASLRQDYERAASFRDRLTALSHIQSRQGINPKSVRE
                     ADVFAAHQDAGQTCIQVFFFRSGNNWGNRAYFPRADRSVPVEEVLESFVAQFYDDKQP
                     PKHVLLSHDIQGRRLLGEALSLRTGRKIDVSTPARGERRELVEHALVNAREALARRLA
                     ESSSQAAILARLAELFDLDESPERIEVFDNSHISGTNPVGAMIVAGPDGFIKGQYRKF
                     NMKSEDLAPGDDYAMMREMLTRRFSRLAKERAEVNGWAAPDLVLIDGGPGQLGAAYAV
                     LDELGLSEIKVAAVAKGPDRNAGRERIYLKGKEPLLLEPRDPVLYCIQKLRDEAHRFA
                     IGAHRAKRKKTITASPLDEVPGIGPRRKRALLQHFGSAKAVSRAGIADLEAVDGISAQ
                     MARTIYDHFHERG"
     gene            8178..8789
                     /gene="pgsA"
                     /locus_tag="VFY74_08930"
     CDS             8178..8789
                     /gene="pgsA"
                     /locus_tag="VFY74_08930"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX5931855.1"
                     /translation="MTQPGLAGPARPRARPRFGSLPNILTYGRILAIPALVACFFVTG
                     DWGRWAAMWIFIAAGVSDFLDGYFARAWQQQSTVGSMLDPIADKLIVAAALLMLAADQ
                     TIAGWSLWAGVIILCREILVSGLREFLATLAVGVPVTRLAKWKTVVQMVAIGFLLAGS
                     AGDKIWPHTTLFGLTLLWIAAIFTLYTGYDYLMAAFRHAMKKE"
     gene            8794..9045
                     /gene="moaD"
                     /locus_tag="VFY74_08935"
     CDS             8794..9045
                     /gene="moaD"
                     /locus_tag="VFY74_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX5931856.1"
                     /translation="MRLLYFAWVKEKAGIAAEDIEPPASVATVAELIDWLKGRGPEFA
                     NAFARAEVIRAAIDHTHVRHDARIKGAREIAFFPPVTGG"
     gene            9042..9500
                     /locus_tag="VFY74_08940"
     CDS             9042..9500
                     /locus_tag="VFY74_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5931857.1"
                     /translation="MSVIRVQQEDFDIGAEVARLKAGRSDIGAIVTFLGTVRDQDGAV
                     AAMTLEHYPGMTEAELARVEAEACARWPLQASLVVHRHGSLKPGDNIVLVVTASEHRD
                     AAFEAARFLMDYLKTRAPFWKREDGPSGARWVEAEARDDASLTRWDKPPA"
BASE COUNT         1647 a         3243 c         3124 g         1637 t
ORIGIN      
        1 aacgaccgca tgttcaaaat gcgagccaag atgatttcgg tgggacttct cggcgccgct
       61 gcgctagtac ctgtcgactt tgccaaggcc gaccagctcg gccacgcttc atggtattcc
      121 cttccagcca ataagaccgc gaacggggag cggatgaatc ccgacgagct cacggccgcg
      181 caccggtcgc tgccgttcgg gacgcgggtc gtggtcgaga atctctccaa cgggcggtcc
      241 gttgtcgtcc gcatcaacga ccgcggcccc ttcatcggcg gccggatcat cgacctgtcg
      301 aaagccgccg ccgccagcat cggcatgatc gccacaggca ccgccaaggt cagggtcacc
      361 actgccgccg gcggcgtgct cgcgggcctg ggcgagtcga cggtcaaggc aaagggcgag
      421 tcgcgcattc atgaggcggc ttctccggca gcgccggaag ccgcggtgca gatcgtttcg
      481 gcgaaaatga ccggcgccac tgccacggag gccaaggctt cgcccaagcc gtcgcagcag
      541 gccaacgttg cgtccaagcc tgccaagacc aagaccctcg ccgtgaccgc atccgcggcg
      601 tcgaagaagc gggtgggttc gcagacgatc aaggtggcag ccgccaagcg atcgcggcca
      661 agcgcaaacg cgaaggctcg cgttcaagtc gtggcggccg ccaacggcct tggtacaggg
      721 cgcgaagctt cgcgcgcctc cagccgccgc gtggtcgtgg cctccaacgg gatcagggcc
      781 gcagcacggg aggcttcgag gttctccagc cgccgcatga tcgtggcatc caacgggatc
      841 aggacggcag cgcgcgaggc ttcgaggttc tcgagccgcc gcgtcaacgt ggcatcgaac
      901 acgctcaatc cttatgccgt ctccggcacc actcccgggt tcacaaaatc cgcccgggtg
      961 cgggcgcggc gcgccatggg ctagcgccct cgagggccgg ccgtcatcct gaggtgcgac
     1021 ctgcctgcgc gaagccggga cttcgctccg gcgaaggcag ggcgtgagcg agcctcgatg
     1081 tatgacattt ccgtaacggc gatccttcga gggccgcttc gcggccgcct caggatgacg
     1141 aatccaatag cgcctaaata gcgcgtacgg gacgaggcac gcattcccct ctcccgtcgc
     1201 gggagagggt ggcgaacctc tcgcgcctcg caggattaca ccccctcacc gcgaagccaa
     1261 acggcgcgcg tgctctccct caaaccgagg ggagagcggg ctttaagtgt gttccggttg
     1321 aggctaatcc tcgaaaacca aaggcgaagt cgtgccggcg agccgcgaga tcgccggcac
     1381 ctgttgcacg gaagccatag gcacgcgcca agaaagaccg catagtggtg ctcttggaag
     1441 cgaaccacaa tatgacgaat cgcttctgga gcacggctca agcctgcgac ggccggtcac
     1501 gcaatcgtaa acttcggccc ggcacatttt tggcggtgat tcgaaacttt tcgaccccaa
     1561 accacgttag aaggccacat gttcaagctc cccgcctccc gcaaattgat cctcaccgca
     1621 gttgccggcg cgttcctcgc gttggtcgca atcgaggacg ccagcgccca gcaggcggcg
     1681 agcggcaatc agtcggcgca aggcgccgga gactatccgc gcagctacgg cccggtcagg
     1741 ccgcgccccg gcggcttttc gcgattaacc ctcagcgata tctggggacc ggcgaagatg
     1801 cctccgcccc cgattgattt cgggccccac ttcgacttcc cgcccgcgcc gctcaatggc
     1861 gctccgctgc acgatcccta tcccaattaa gcgttttcgc atgcgcactg acggcgccga
     1921 aaccggccag atgggtcttg cgttgggcgg tctggcgccc ctagagtgag gcgctttgcg
     1981 ggcagactgc gaactcgggt ggagattggc cgcatgcgct ccgtgggagg cgaggcggtt
     2041 tcttttcccg ctttctagac ttgcctgaat ttctgcgagg gcctttagcg cgcagaccat
     2101 gtgaaagggg aaagggagct tcccatgcgt cagaccggta cggtcaagtt cttcaatcac
     2161 tcacgagggt tcggcttcat cacgccggac gatggcggca aggatgtgtt tttgcatatc
     2221 tcggcgttgc agcgctcggg ccttcccgcg ctcgacgagg gcgcccgcgt ctctttcgag
     2281 accgagccgg accgccgggg caaggggccg caggcgatca acgtgcagat gggcgaatag
     2341 tcctcccctt acattcggac gctacatcga tcggctccgt tagcggcggc atgcacgctg
     2401 ccgccgggaa cgcgatgacg cattggcgcg ccgttctcac gctcgagtgc gccgcggccg
     2461 aggcatggcg atgaatgaat ccgaggcgcc ggtgagcgct ttgcaaagcg tgctcggccg
     2521 gctcgcccgc gaggagccgc gcgcgctcga gcggctgttc gggctgctcg ccatccccag
     2581 cgtctcgacc gatcctgcct atcaccaagc atgcgtcgac gcggcggaat gctgcgccgg
     2641 tctcttgcgc gaggcaggtt tcgatgcgcg ggtggtgccg acctccggca agccgatggt
     2701 ggtcgggcat tcgcgcgcac gtccttccgc caagccgcgc cgccacgcgc tgttctacgg
     2761 tcactacgac gtgcagccgc cggagccttt cgaggcgtgg accgtgccgc cgttccagcc
     2821 gcatctcgcc gacgatccgc atcacggcaa ggtcatcgtc gcccgcggcg cctccgacga
     2881 caagggtcag atgatgactt tcatcgaggc cgcgcgggcc tggcgcgacg agcttggaac
     2941 gctgccgctc gacgtcagcg tgctgatcga gggcgaggaa gaatccggaa gcccgagtct
     3001 tgcaccgttc ctcgctgccc acggcgatga gctcaaggcc gatctcgcgc tcgtctgcga
     3061 taccgcgcag tgggatgcag agacccccgc catcacagcc ttcctgcgcg gactggcctt
     3121 ctccgagatc accgtgcgcg gcccgtcgcg cgacctgcat tccggcgtct acggcgggcc
     3181 tgccctcaat cctttgcgcg ttctgtccga gattctcgcc gcgcttcacg acggcgaggg
     3241 ccgcgtcgcc attccaggtt tctatgacgg catccgcgcg ccctcccccg ctgaactcgc
     3301 gagctggcgc gcgctcggct tcgacgaggc ggggtttctc ggcgacgtgg gattgcgtga
     3361 acccgccggc gagaaaggcg tctccatgct cgagcagctc tggtcgcgtc cgaccgccga
     3421 gatcaacggc atcgtgggcg gctatggcgg accgggcacc aagacggtga tccctgcaca
     3481 ggcaaccgcg aagctgtcct tccgtctcgt ccccgggcag gatccggcac acgtcatcgc
     3541 gtgctttcat cgcttcgttt cggcgcggtt gccggcggat gcgaaggtgt cttacgcgca
     3601 tgagggcgga tcgcctgcga tcggtttcga cacaagcgcg ccggccttcg ttgcggccgc
     3661 ccgcgcgctc gaagccgagt ggggcaaggc gcccgcgatc atcggctgcg gagcctccat
     3721 cccgatcgtc gagtccttcc ggtctcgcct cggcatggac tcgcttctcg tcggctttgc
     3781 gctcgacgac gaccgcatcc acgcgccgaa cgagaaatac aatctccaaa gcttcaccaa
     3841 ggggatgcgg agctgggtgc gtattctggc cgagcttggc gcgctcgcgt gatgcggtcg
     3901 ggtcttacgc ggtccctgcc cctgcccctg gccctggccc tcgcgctcgt gtctctgacc
     3961 ggggcaagcg gcgccgcggg cgcggaacgc aacacgccgg gcgcgttcga ttactatgcg
     4021 ctcgttctca gctggatacc gtcctactgc cggggcgagg gcaaaggccg caaggacaag
     4081 caatgcgacg gcaccaagcc gcatgccttt ctgctgcatg ggctatggcc gcaacacgaa
     4141 atcggctggc ctctcgattg ccgcacggtc aagcgcccct gggtgccgga aagcgtgatc
     4201 gccgagatgg gcgacatcat gccgagcaag aatttggtga tccatgaata tcgcgcacac
     4261 ggaacctgtt cgggtctcga gcccaaggaa tatttcgcgg ccgcgcgcgc tctctatgag
     4321 cgcatcagcg ttccggcggc ctttctcgcg cctggcgccg acctcgactc tcccgaggag
     4381 atcgagcggg cctttctcga cgccaaccct tggctcacgc cggacatgat ctcgatcacg
     4441 tgccggaaag cgcatcttct cgatatcagg gtgtgctttg gccgcgatct cagcccgcgg
     4501 aactgcggcg tcaacgagga tcagaggcgg ctttgcgccg cgccgaaggt tgtcgtgccg
     4561 cccccggcgc agcgctgacc gtaagctcag ttcgcgcgaa agatggcgac gcagccctct
     4621 tgcgcgatcg cgacatcggc gaagcgttgc cgcaatccgt ccgacaggga agcgagatcg
     4681 tcctcgcgat tgttgaacac gccttgcgca ttgtagagat cgagcagcgc cgaagcggcg
     4741 cggttcggcg cgatcccgcg cccgaggatc gtcgcgccga atagcgtggc gccatcgcgc
     4801 atcagcggac gcaaatggtc caccgctttc agcttctcgt ccatgcggcc gggtaggcaa
     4861 tgaagcacat aggtcagccc gacggaggcg acgggcgccg tgtcgaacgc gaccggctga
     4921 agcaggtcga cctgatgaag ttcgaggtcg aagcgggcga gcctcagcgc ggagcggtcg
     4981 agacaattgc gattggcgtc gagcaggacg agccgtttca gccgctccgt cccggcgcga
     5041 tcgagcagca cgccggtgcc gacaccggcc tccaaatggt caggagacag attgtcgcga
     5101 tagagttcca cgagacgccg cgtcgggcag cgccaggcga aacgattgga caggccgtgg
     5161 acgagcacgt cgtaaatgga gagggcgaag ggcgtataga ccgcatgcgc cctcgcgacg
     5221 gcgttctcgt caagcacggc ctcacctctt cgcaaaactc tcccggtctc aataggacgc
     5281 gggaggcgcc gaggaaaggg cgagccttgt tcgcatggag gggctcactt agagtagccc
     5341 gtgcacccgg ctctctcacc gacccatctc taacgcgacg gtatggcgaa agacaaacac
     5401 gaccggaccg tgctcgtgac cggcgcggcg cgaaggctcg gccggatcat cgcgctcgat
     5461 ctcggcgccc gcggctggcg cgtcggcgtg cattatcgcg cttccgcggc cgaggccgcc
     5521 gccctcgtgg cggagatcga acgcaatggc gggacggcag ccgcgcttgc cgccgatctc
     5581 gaccgcctgg acgcgctttc aaagttgatc gcggattgcg cctcttcgct cggtcctccc
     5641 gcctgcctca tcaacaatgc cgcgcgtttc gagcacgaca cgctcggcac gcttcagggt
     5701 gaaacgtggc aggcgcacct cgatgtgaat ctcagggctc cgatcttttt gactcaggcc
     5761 ttcgccagga ccctgcccga cggcgtcgaa ggaaacgtca tcaatatcat cgaccagaaa
     5821 gtcctgcgcc ccgaccctga ctatttttct tacaccatgg ctaaggcggc gctttggtcc
     5881 gcgacgcaga tcatggcgca ggcgctcgct ccgcggatca gggtgaatgc catcgcgcca
     5941 ggtcccgtgc tcaatgccga cgggcaaagt gagagcgcgt tcgaacggga gtatcgcgag
     6001 accttgctgc aaagggccgt cggcaccgac gatgtctccc gcgccgtccg gttcctgctc
     6061 gaaacgccct cgatcaccgg ccagatcatc gcactcgaca gtggccagca tctggttttc
     6121 aaggccaaaa gcggcgacca ttgaggctgc ctcacaggct cttgaaggag gcgaggccaa
     6181 aaccctacct tcgcttgtgc aagcgcatgc cgcaggcatg tgcgaagatc gatgatccca
     6241 tgcccttaga gaaccccgac cccgccggat ccacccgcct tgcgcgcgcg ctcgatgcgc
     6301 tgcgcgccgt cgagaccggc gcgccggtga tcggccgcca tgccaagacg gcgccggccg
     6361 gacccggcgt ctaccgcatg atcgatgccg cgggcgaggt gctctatgtc ggcaaggcgc
     6421 ggcatctgaa gaagcgggtg cagagctacg cgcggggggc cggccacaac aaccgcatcg
     6481 cccgcatggt cgccgacacc gcgagcatgg aattcgtcac cacggcgacc gagatcgagg
     6541 cgcttctgct cgaggccaat ctcatcaagc ggctcaagcc ccgcttcaac gtcgtgctca
     6601 gggacgacaa gtccttcccc ttcattctca tcgcgcgcga ccatccggtg ccgcagatcc
     6661 tgaagcatcg cggcgcgcgc aaccgcaggg gcgactattt tggtcctttc gcttcggccg
     6721 gcgcggtcgg ccgcaccatc aacacgctcc agcgcgcgtt tctgttgcgc tcgtgctccg
     6781 acgcctatta cgagagccgc acgcggccgt gcctgctctt ccagatcaag cgctgcagcg
     6841 cgccttgcac cggggagatc tcgctcgccg attacggcgc gctcgtcgac gaagcgctcc
     6901 gcttcctcac cggcgagagc gacgaggtga agctgaggct ccgcgcgctg atggacgagg
     6961 cgagcctccg ccaagactac gagcgggcgg cgagctttcg cgaccgcttg acggcgctca
     7021 gccacatcca gtcgcgccag gggatcaatc cgaagagcgt gagggaggcc gacgtgttcg
     7081 ccgcccacca ggacgccggc cagacctgca tccaggtttt cttcttccgc agcggcaaca
     7141 attggggcaa ccgcgcctat tttccgcgcg ccgacaggag cgttccggtc gaggaggtct
     7201 tggaatcctt cgtcgcccag ttctatgacg acaagcagcc tccgaaacac gtcctgctct
     7261 cgcatgacat acaagggagg cgcctgctcg gcgaagccct gtcgctgcgg accggccgca
     7321 agatcgacgt gagcacgccc gcgcggggcg aaaggcgcga actggtggag cacgccctcg
     7381 tcaatgcgcg cgaggcgctg gcgcggcgcc tggccgaatc ctcgtcgcag gccgcgatcc
     7441 tggcgcggct tgccgagttg ttcgatctcg acgaaagtcc agagcgtatc gaggtcttcg
     7501 acaacagtca catttccggc acgaatccgg tgggcgccat gatcgttgcg gggccggacg
     7561 gtttcatcaa agggcagtat cgcaagttca acatgaagag cgaggatctg gcccccggag
     7621 acgattatgc gatgatgcgc gagatgctga cgcgccgctt cagcaggctc gccaaggagc
     7681 gggcagaggt gaacggctgg gccgcgcccg atctcgtgct gatcgatggc ggccccggcc
     7741 agctcggcgc cgcttacgcc gttctggacg agcttggcct ttccgagatc aaagtggccg
     7801 ccgtggccaa ggggcccgat cgcaacgccg ggcgggagcg aatctatctt aagggaaagg
     7861 agccgcttct gttggagccg cgcgaccccg tgctctattg tatccagaaa ctcagggacg
     7921 aagcgcatcg cttcgccatc ggtgcccatc gcgccaaacg caaaaagacg atcaccgctt
     7981 cgccgctcga cgaggtgccc ggcatcgggc cccggcgcaa gcgggcgctg ttgcagcatt
     8041 tcggctcggc caaggcggtc agccgcgccg gcattgccga tctcgaagcg gtcgacggta
     8101 ttagcgcgca aatggcgcgg acgatctatg atcacttcca tgagcgcggg taggtcgcgg
     8161 tgaaccagca gtcggacatg acgcagccag gccttgcggg gccggcgcgc ccgcgcgccc
     8221 gtccccgttt cggcagcctg ccgaacatcc tgacctacgg gcgcatcctc gccattccgg
     8281 cgctggtcgc ctgcttcttc gtcaccggcg attgggggcg ctgggccgcc atgtggattt
     8341 tcattgccgc cggcgtcagc gatttcctcg acggctattt cgcgcgcgcc tggcagcagc
     8401 agagcacggt gggcagcatg ctcgatccca tcgccgacaa gctgatcgtg gcggcggcgc
     8461 tgctcatgct cgccgccgat cagaccatcg ccggctggtc gctctgggcc ggcgtcatca
     8521 tcctctgccg cgaaatcctg gtgtcgggct tgcgcgagtt cctcgccacg ctcgccgtcg
     8581 gcgtgccggt gacgcggctc gccaaatgga agaccgtggt ccagatggtg gcgatcggat
     8641 tccttctggc cgggagcgcc ggtgacaaga tctggcctca caccacgctg ttcgggctga
     8701 cgctgctgtg gatcgccgcc atcttcacgc tctataccgg ctacgactat ctcatggccg
     8761 ccttccgcca tgcgatgaag aaggagtagc gcgatgcggc tcctctattt cgcctgggtc
     8821 aaggagaagg ccggcatcgc cgccgaagac atcgagccgc ccgcgagcgt cgccaccgtc
     8881 gccgagctga tcgattggct gaagggccgc gggccggaat tcgccaatgc ctttgcccgc
     8941 gccgaggtga tccgcgccgc gatcgatcac acccacgtgc gccacgacgc gcggatcaag
     9001 ggcgcacgcg agatcgcatt cttcccgccc gtgaccggag gctgagcgtg atccgggtcc
     9061 agcaggagga tttcgatatc ggcgccgagg tcgcgcggct gaaggctggc cggagcgaca
     9121 ttggcgccat cgtcaccttc ttgggcacgg tgcgcgatca agacggcgcc gtcgcggcga
     9181 tgacgctcga acattatccg ggcatgaccg aagcggagct cgcccgcgtc gaggccgagg
     9241 cttgcgcgcg ctggccgctg caggcgagcc tcgtcgtgca tcgccatggg tcgctcaagc
     9301 ccggcgacaa tatcgttctc gtcgtgaccg cgtccgagca tcgcgacgcc gcgttcgagg
     9361 cggcgcgctt tctcatggac tatctcaaga cccgcgcgcc gttctggaag cgcgaggacg
     9421 gcccgtccgg tgcgcgctgg gttgaggccg aggcgcggga cgacgcctcg ctgacgcgct
     9481 gggacaagcc gccggcctga gcagcctgtg cacgcgccct tgctctgcct ccccctcgtg
     9541 gggaggtcgg cacgcttagc gtgccgggtg gggggatggt atttcaacgc acgactattt
     9601 cgccggttaa atccgcgccc cccaccccgg cgcgccatgc gctcaagtag c
//
LOCUS       DASUQP010000200         7021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_235781,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000200 DASUQP010000000
VERSION     DASUQP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235781"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFY74_08945"
     CDS             <1..1118
                     /locus_tag="VFY74_08945"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5931858.1"
                     /translation="AGRPLPSGYRLKNPALAETLETIARDGPDTFYRGAIARDIADAV
                     QRDPRRPGNLTTDDLARYRAIEREPVCVPYRLHRVCGMGPSSSGGVTVGQVLALIEFY
                     DLGPAPLGAHAAHLIVEAERLAYADRALYLADPDFVSVPVAGLLDADYLAERRALIDP
                     TRAREHVAAGLPPNTKQGSFGLDRTHERKGTSHISVVDGHGNAFSMTTSIEQAFGART
                     MVRGFLLNNQLTDFSFAAADEDGRAIANRVEGGKRPRSSMDPTIVFGPDGLRYVLGSP
                     GGSAIIFYNVKTIVALIDWGLDPAEALALINFGSIEKAVLLEPGAAWDDLAASLATMG
                     HEVRRVPLTSGEHVIAITRQGLEGGADPRREGVALGD"
     gene            1118..2128
                     /locus_tag="VFY74_08950"
     CDS             1118..2128
                     /locus_tag="VFY74_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5931859.1"
                     /translation="MTRRSYAIAVRGAGVVGLWQALVLARRGHAVRLYEQSAQPFAGA
                     CSVYAGAMLALRCEEDNAEPVVNELGKRGIALWRETYSGTAVNGTIAVALGRDRAELD
                     RFARTTVGHQRLSPAGLAALEPSLGNRFASALFFPEEAHLAPEAALRFLLDRAQAEGV
                     TLRLDDGAAPKGAEIVVDCRGLGAKDDLPNLRGVRGERIVVKSRDVTLTRPVRLLHPR
                     FPIYVVPWGDGLYMIGATVIESEEAGPITLRSALDLLSAAYALDPAFAEAEIVAQGAG
                     ARPAFPDNRPRIIARNGYIYVNGLYRNGFLLAPALAELVADYVETGAKDPEVFVEDPA
                     QR"
     gene            2106..2303
                     /gene="thiS"
                     /locus_tag="VFY74_08955"
     CDS             2106..2303
                     /gene="thiS"
                     /locus_tag="VFY74_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX5931860.1"
                     /translation="MKILLNGEAFATDAKTLDELCARLGFAGAKIATAVNGSFVAASS
                     RGATTLADADEIEIVAPRQGG"
     gene            2303..3091
                     /locus_tag="VFY74_08960"
     CDS             2303..3091
                     /locus_tag="VFY74_08960"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5931861.1"
                     /translation="MLTLYGKTFTSRLLLGTAQYPSPHSLKQAVEASGAEIVTISLRR
                     ESSRARTGQGFWALIEEMGCRVLPNTAGCHTAKEAIATAKMARELFGTDWVKLEVIAN
                     DDTLQPDLFGLVEAAAALSSEGFEVLPYTTEDLSAAERLVAAGCTVLMPWAAPIGSAR
                     GLINKEALKTLRAYFPDIPLIVDAGLGAPSHAAEVMEMGYDGVLLNTAVAKAGDPVKM
                     AEAFAKAVAAGRLAFEAGLIEPRDMASPSTPVAGTPFFKIDQAS"
     gene            3118..3747
                     /locus_tag="VFY74_08965"
     CDS             3118..3747
                     /locus_tag="VFY74_08965"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5931862.1"
                     /translation="MRLDPFYPILPDTAWLARLLPHGIKLAQLRLKDKPQRVISAEIE
                     KAMALCDAHRCQLVVNDYWREAIDAGAGFVHLGQEDLAAADLPAIRGAGVKIGVSTHS
                     RAELDAALAAGADYVALGPIYATKLKAMPWAPQGLPRIAEWRAGISCPLVAIGGISLD
                     RVPIVLEAGADSAAVITDIVTSEDPDLRVKNWIAATAPWRKLSTDIAQA"
     gene            3826..4797
                     /locus_tag="VFY74_08970"
     CDS             3826..4797
                     /locus_tag="VFY74_08970"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX5931863.1"
                     /translation="MSYQLDPALPMSDALRRAAFAELELAHGALAAPPERHSGVHNAR
                     KCLKRLRSLLLLARPGMPEPAFRTLTDRLADIAKGLAAARDAAALSDAIDKLERETGA
                     GPGLGPLRSLRAWLHKRRHAAEQNLAESAASDAMRGLLEIRPAFAGVAVYPDDFRPLS
                     RGLRRCYRATRNAFRHAVDTARDEDLHEWRKGVQHHWRQMQLLAPCWPSELSARAEAA
                     KALSQILGDDHDISLLIRLISTPTMTFGTAEETAAFLKRCRRRHKTLRKEAHVQGEKL
                     FAERSRPFAERIETYWQIAAGDAAKATAEVRPDNVVAFGDLRAPRAS"
     gene            complement(4838..5770)
                     /gene="pqqB"
                     /locus_tag="VFY74_08975"
     CDS             complement(4838..5770)
                     /gene="pqqB"
                     /locus_tag="VFY74_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEX5931864.1"
                     /translation="MLIKVLGSAAGGGLPQWNCNGRNSADARKRTPGLPPRTQASVAV
                     TADGSQWVLLNAAPDLRQQINDTPELHPVSDGPPRNSPIKAVVLTNGDVDAVAGLLTL
                     REGQPFTVYGTARVLGVLASNTIFDVLDKRLVKRVVMEFGRLFAVEGPSGPLGLTIEA
                     FPVPGKVPLYLEDATARGNLGTQEGDTAGLKITDTSSGRHFFYIPGCARLDDALRARL
                     KGAPLLFFDGTLYTNDEMIAQGLLNKTGERMGHMNMSGSAGSIAEIAPLAIGRKIFIH
                     INNSNPALREGSPERAAVERAGWEVAYDGMEVRV"
     gene            complement(5873..6769)
                     /locus_tag="VFY74_08980"
     CDS             complement(5873..6769)
                     /locus_tag="VFY74_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931865.1"
                     /translation="MGQQRTSLRGAQRLALSAAVAVLASAGLTVGATTSVLAEFEIEE
                     AGIEKREKEFEYRGAVHEGFPKAEREAAEEGGGGGALEEEEEGEFLRQSHDFEFHYGI
                     TERLGFGTTLITNEPLDGSYDLSAVEVELQYELIQREGNGLGLAFLGGYGIATRGGEA
                     DEIEFGPIVELAAGRALLTINPFFTSQVGDNRETDGLGFEYGWRGQYAIARHWGVGVE
                     MFGEIEDLSNAGSFDDQPHSIGPTLFYNPGEEEDEGGNGDDDDKIAQGPEMEISFNVG
                     VQFGLTDATSDTAVKFQGSLAF"
BASE COUNT         1189 a         2358 c         2211 g         1263 t
ORIGIN      
        1 aggcgggaag gcctttgcca tcgggctacc ggcttaagaa ccccgctctc gccgaaacac
       61 tcgaaacgat cgcccgcgac ggacctgata cgttctaccg aggcgccatc gcccgcgaca
      121 tcgccgacgc ggtgcagcgc gatccccgca ggccgggcaa cttgacgacc gacgatctcg
      181 cccgctatcg cgccatcgag cgcgagccgg tctgcgtgcc ctatcgactt catcgcgtct
      241 gcggcatggg tccatcctca tcaggcggcg tcaccgtggg ccaagtgctc gcgctcatag
      301 aattctacga cctcggtccc gcgccgctgg gggcgcacgc cgctcatctc attgtcgagg
      361 cggagcggct cgcctatgcc gatcgcgcgc tttacctcgc cgaccccgac ttcgtttccg
      421 ttcccgtggc gggtctcctc gatgccgact atctcgccga gcggcgcgct ctgatcgatc
      481 cgacgcgcgc gcgcgagcat gtcgcggccg gtctcccgcc aaacacgaag cagggctcct
      541 tcgggcttga tcgcacgcat gagcggaagg gaacaagcca tatctccgtc gtcgacggtc
      601 atggcaacgc cttctccatg accacgagca tcgagcaagc cttcggcgcg cggaccatgg
      661 tgcgcggctt tctgctcaac aatcagctca ccgacttctc ctttgctgcc gctgacgaag
      721 acggccgcgc catcgccaat cgcgtcgagg gcggcaagcg tccgcgcagt tccatggacc
      781 cgaccattgt gttcggaccc gacggtcttc gttacgtgct tggcagcccc ggcggctcgg
      841 cgatcatttt ttacaacgtc aagaccatcg tcgccttgat cgattggggc ctcgaccctg
      901 cggaagcgct cgcgctaatc aatttcggca gcatcgagaa ggcggttctg ctcgagcccg
      961 gggcggcgtg ggatgatctt gccgcctctc ttgccaccat gggccatgag gtacgccgcg
     1021 ttcctctgac cagcggcgag catgtcattg ccataacgcg ccaagggctt gagggcggcg
     1081 ccgatccgcg ccgcgaaggc gtggcgctgg gggactgatg acgcgccgga gctacgcgat
     1141 tgccgttcgt ggtgccggcg tggtcggcct ctggcaggcg cttgtgcttg cccggcgcgg
     1201 ccacgccgtt aggctttatg agcagtcggc gcagcccttc gccggcgcgt gcagcgtcta
     1261 tgccggcgcc atgctcgcgc tccgctgcga ggaggataac gccgagcccg tcgtgaacga
     1321 gctcggcaag cgcggcatcg cactctggcg cgagacctat tccggcacgg ccgtcaacgg
     1381 cacgattgcc gtggcactcg gccgcgaccg ggccgagctt gatcgcttcg cccgcacgac
     1441 ggtcgggcac cagcgattgt caccggccgg gcttgcggca ctggagccgt ctctcggcaa
     1501 ccgcttcgca agcgcgctct tcttccccga ggaagcccat cttgcgcccg aggccgcctt
     1561 gcgtttcctg ctcgatcgcg cccaggcgga aggggtgacg ctacgcctcg atgacggcgc
     1621 tgcgcccaag ggtgccgaaa ttgtcgtcga ttgccgcggc ctcggtgcca aggacgatct
     1681 tccgaacctg cgcggcgtgc gcggcgagcg gatcgtggtc aagagccgcg atgtgacgtt
     1741 gacgcgtccg gtgcgcctgc tgcatccgcg ctttcccatc tatgtcgtgc cctggggcga
     1801 cggactttat atgatcggcg caacggtgat cgagagcgag gaggcaggcc cgatcacgct
     1861 tcgctcggcg ctcgatctcc tttcggccgc ttatgccctc gatcccgcct tcgcggaggc
     1921 cgagatcgtc gcgcagggcg ccggcgcgcg acctgccttc cccgacaatc ggcctaggat
     1981 tatcgcgcgg aacggctata tctacgtcaa cggtctctac cggaatggat tcctgctcgc
     2041 ccccgctctg gccgagctgg tcgccgacta tgtcgagacg ggtgcgaagg acccggaggt
     2101 gtttgttgaa gatcctgctc aacggtgaag ctttcgccac cgatgcgaag accctcgacg
     2161 agctttgtgc gcggctcggc tttgccgggg cgaagatcgc gaccgccgtg aacggaagct
     2221 tcgtcgccgc ttcctcgcgc ggtgcaacca cgctcgccga tgccgacgag atcgagatcg
     2281 tggcgccgag gcagggcggc tgatgctcac gctttacggc aagactttca cttcgcggct
     2341 cctgctcggc acggcgcaat atccctcgcc acacagtctc aagcaggcgg tcgaggcgtc
     2401 gggcgccgag atcgtcacca tctcgcttcg ccgggaatcg tcgcgcgccc gcaccggcca
     2461 gggattctgg gcgctcatcg aggagatggg ctgccgcgtg ctgccgaaca ccgcgggctg
     2521 ccacacggcg aaagaggcga ttgccacggc caagatggcc cgcgagctgt tcggcacgga
     2581 ctgggtcaag ctcgaagtca tcgccaacga cgatacgttg cagcccgatt tgtttggcct
     2641 cgtcgaagcc gccgccgcgc tctcgagcga aggcttcgag gtgctcccct atacgaccga
     2701 agatctgagc gcggccgaac ggcttgtcgc ggccggttgc accgtgctga tgccgtgggc
     2761 ggcgccgatc ggttcggcgc gcggccttat caacaaggag gcgctcaaga cgctccgcgc
     2821 ctacttcccc gacatcccgc tcatcgtcga tgccggactt ggcgccccct cgcatgccgc
     2881 cgaagtcatg gagatgggtt atgacggtgt gctgctcaac acggccgtgg ccaaggcggg
     2941 cgatccggtg aagatggcgg aagccttcgc caaggccgtc gcggccgggc ggcttgcctt
     3001 cgaagccggg ctgatcgagc cgcgcgacat ggcctcgccc tcgacgccgg tggccggcac
     3061 gcccttcttc aagatcgatc aggcctcttg atcgatcctg ggtcaggccc cgctttgatc
     3121 cggctcgatc ctttttaccc gatcctcccc gatacggcgt ggctcgcccg tctcctgcct
     3181 cacggcatca agctggcgca gcttcggctc aaggacaagc cgcagcgcgt catcagcgcc
     3241 gagatagaaa aggcgatggc gctctgcgac gcgcatcgtt gccaactcgt ggtcaacgac
     3301 tattggcggg aagcgatcga cgccggcgcc ggcttcgtcc atctcggcca ggaggatttg
     3361 gctgccgccg accttcccgc aatcaggggc gcaggtgtga agatcggtgt cagcacgcat
     3421 agtcgcgcgg agttggatgc ggcgcttgcc gccggcgccg actatgtcgc gctcggtccg
     3481 atctacgcga ccaagctgaa agccatgccc tgggcgccgc agggtctgcc ccgaatcgcg
     3541 gaatggcgag ccggtattag ctgtcccctg gttgcaatcg gcggcatttc actggaccgc
     3601 gtgccgatcg tgcttgaggc gggcgccgat tccgccgcgg tcataaccga cattgtgaca
     3661 agcgaagatc ctgatttgcg cgtgaaaaac tggatcgccg cgaccgcgcc gtggcgcaag
     3721 ctatccacag acattgcaca ggcctgattc ggcggtttga aacttgaccc ggaccggccc
     3781 ccgaaaggat tattgcgtcg gaagcgcccc aagccgagga gcctcatgag ctatcaactc
     3841 gatccggccc tgccgatgag cgatgccttg cggcgcgcgg cctttgccga gctcgagctt
     3901 gctcacggtg cgctggcagc cccgcccgaa cgccatagcg gcgtccacaa cgcccgcaaa
     3961 tgtttgaagc ggcttcgctc gctgcttctg ctggcgaggc ccggtatgcc tgagcctgcg
     4021 tttcggactc tgaccgaccg gctggcggac atcgccaaag ggctggctgc ggcacgcgat
     4081 gccgccgcct tgagcgacgc catcgacaaa ctcgaacgcg agacgggggc ggggccgggt
     4141 ctcggtccgc tgcgttcgct gcgcgcgtgg ctgcacaaaa gacggcacgc ggccgagcag
     4201 aatctcgcgg agagcgccgc ttcggacgcg atgcggggtc tgctcgaaat cagaccggcc
     4261 tttgccgggg tcgccgtcta tccggatgac ttccgccccc tttccagagg ccttaggcgc
     4321 tgctaccgcg ccacccgtaa cgcgtttcgg cacgcggtcg acaccgctcg cgatgaagat
     4381 ctgcatgaat ggcgcaaagg cgtgcagcat cattggcggc agatgcagct gctggcgccg
     4441 tgttggccgt ccgagctttc ggcgcgggcc gaagccgcca aggcactctc gcagatcctc
     4501 ggcgacgacc acgacatttc attgctcatc cggctcattt cgacgccgac gatgacattc
     4561 ggcacggccg aggaaaccgc agccttcctc aaacgctgcc gcaggcgcca caaaaccttg
     4621 cgcaaagagg cacatgtcca gggtgagaaa ctgttcgccg agcggtcgcg gccgttcgcc
     4681 gagcgcatcg aaacctattg gcagattgca gccggcgatg ccgccaaggc cacggccgaa
     4741 gtccggcccg acaatgtggt ggcgttcggc gacttgcgcg cgccgcgcgc aagctagagc
     4801 gtatcgacgg tccggtttgc cccggcaacg gcggccgtca gaccctcacc tccatcccat
     4861 cataggcgac ctcccagccc gctctttcga ccgccgcgcg ctcgggcgag ccctcacgca
     4921 aggcgggatt ggagttgttg atgtggatga agatctttcg cccgatcgcg agcggagcga
     4981 tctcggcgat gctgcccgcg ctgcccgaca tgttcatatg acccatgcgt tcaccggtct
     5041 tgttgagcag tccctgggcg atcatttcgt cattggtata gagggtcccg tcgaagaaga
     5101 ggagcggcgc gcctttgagc cgcgcgcgaa gcgcatcgtc cagcctggca cagcccggga
     5161 tgtagaagaa gtggcggcca ctcgatgtgt cggtgatctt gagacccgcc gtatcgccct
     5221 cctgcgtgcc gagattgccc ctcgctgtcg cgtcttcgag atagagcgga accttgcccg
     5281 gcaccgggaa ggcttcgatg gtcaggccga gcggaccgga agggccttcc acggcgaaga
     5341 ggcgcccgaa ctccatcacg acccgcttga ccagccgctt gtcgagcaca tcgaaaatcg
     5401 tgtttgaggc gagaacgccg agcacgcgcg cggtgccgta gacggtaaag ggctggccct
     5461 cgcgcaatgt caaaaggccc gccaccgcat cgacatcgcc attggtgagc actacagcct
     5521 tgatcgggct gttgcgcggg ggaccatcgg agacgggatg aagctccggc gtatcgttga
     5581 tctgctgacg caggtcggga gccgcgttga gcaaaaccca ttgcgacccg tcggcggtca
     5641 cagcgacgga ggcttgggtg cgcggcggca gccctggcgt gcgcttgcga gcgtccgccg
     5701 agttgcggcc gttgcaattc cactgcggaa ggccgccgcc tgccgccgag ccgaggactt
     5761 tgatcagcat gacgaaggat cagatggcga ccggcgcccc gccccgtcaa ggcaaaaaga
     5821 caacggcctc agcgtgctac gaagcccatc ctgccgcttg ggcttcgcgc gcttagaacg
     5881 cgagcgagcc ctggaatttg accgccgtat cggaggttgc gtcagtcagg ccgaactgga
     5941 cgccgacatt gaaggagatt tccatctccg gtccttgcgc gatcttgtcg tcgtcatcgc
     6001 cgtttccgcc ctcgtcctct tcctcgccgg ggttgtagaa cagggtcggg ccgatgctgt
     6061 gaggctggtc gtcgaacgag ccggcattgg agagatcctc gatctctccg aacatctcca
     6121 cgccaacgcc ccagtgccgg gcaatcgcat attggccgcg ccagccatat tcgaaaccga
     6181 ggccatcggt ttcgcggttg tcgccgacct gagaggtgaa gaacggattg atggtaagta
     6241 acgccctgcc ggccgccaat tcgacgatgg gtccgaattc gatttcgtcg gcttcgccgc
     6301 cccgcgtggc gataccatag ccgccgagga acgcaagtcc tagcccgttg ccctcgcgct
     6361 ggatcagctc atattgaagc tcgacctcga ccgcactcaa atcgtagctg ccgtcgagcg
     6421 gctcgttagt gatgagggtg gtcccaaacc cgagccgctc ggtaatgccg tagtggaatt
     6481 cgaagtcgtg gctttgccga aggaattcgc cttcctcttc ctcctcgagg gcgccgcctc
     6541 ccccgccctc ctcggccgcc tcgcgctcgg ccttggggaa cccctcatgg acggcgccgc
     6601 gatattcgaa ctccttttcg cgcttctcaa ttccagcttc ctcgatttcg aactcggcga
     6661 gaacggatgt ggtcgcgccc acggtgagcc ccgccgaagc aagcaccgca accgcagccg
     6721 acagcgccaa gcgctgcgcg ccacgcagtg aagtccgctg ctgtcccaag agccgccccc
     6781 gactttgagc cctgccaaaa ccctcgattc ttatggccta gcttagcaaa tctccccccg
     6841 gcgccaacgg caaaaaactg ccgcttcccc actggtcatg acaccgaggc tgtcgcccat
     6901 gccactctga aaataaccgg aggaaaatca ccatggcgat cggaagatgg ccccaagtat
     6961 agggaaactg cgttatagaa aaccggccat tttttgcggt gcggcccggc ttcgcccaag
     7021 c
//
LOCUS       DASUQP010000201         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_372596,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000201 DASUQP010000000
VERSION     DASUQP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372596"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..2557)
                     /gene="ligA"
                     /locus_tag="VFY74_08985"
     CDS             complement(407..2557)
                     /gene="ligA"
                     /locus_tag="VFY74_08985"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5931866.1"
                     /translation="MSKTKPRKTAAAGPALEDKPSAKLDEAEAATELARLAASIAHHD
                     ELYYRKDQPEISDAEYDGLRARNQEIEARFPHLVRDDSPSLKVGAAPVEAFGKVTHRM
                     PMLSLGNVFDEAGLRDFLDRIRRFLGRDTIEGLAFTAEPKIDGLSITLRYEGGKLVQG
                     ATRGDGYEGENVTANVRTIADIPKTIKSRGFPDPFELRGEIYMSRAAFQRLNEEQARV
                     GDRLFANPRNAAAGSLRQLDPAITAKRPLRFFAYGWGEVAGGFSAETQWDVYQLMRAW
                     GFPLNPLIRLTRSVEEMLETYRDIESGRAGLDYDIDGIVYKLDRLDLQQRLGFVSRSP
                     RYAIAHKFPAEKATTILREIDIQVGRTGALTPVAKLDPVTVGGVVVQNATLHNEDEIA
                     RKDVRKGDTVIVQRAGDVIPQILGVVEEKRPKGAKRFVFPKFCPACGSHASREINPNT
                     GKEDAVRRCTGGLICPAQRVERLKHFVSRNAFDIEGIGEKHIKSFYDDGLIQSPPDIF
                     TLLARDKRASAKLADREGWGETSAKKLFDAIDARRSIRLDRFIYALGIPHVGETTARL
                     LARDYGTIERFMASMGAAAKGRESEAFAELDSIEGIGPTVAAAIADFFAEPHNVTVVK
                     ELLREVTPEPLEAVDHASPISGKTVVFTGTLEQMTRSEAKARAERLGAKVAGSVSKKT
                     DYVVAGPGAGSKLKDAKDLGLTVLTEDEWLKLIH"
     gene            complement(2567..>2824)
                     /locus_tag="VFY74_08990"
     CDS             complement(2567..>2824)
                     /locus_tag="VFY74_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX5931867.1"
                     /translation="ERLARLAARVQVIAVTHAPQVAAVASGHLLIAKEPVRGKAGEAM
                     ATRVAVLEGDQRREEIARMLAGQTITEEARKAAERLMSRTG"
BASE COUNT          438 a          905 c          918 g          563 t
ORIGIN      
        1 gggcggaatg atcgaccatg agagatgctc ctcttcggac gtcgcgttag aggtccgctc
       61 cgggctcaac cacgaatgga tcgatgaccc ttagcccatt gagatcttga tcgtgattgg
      121 ttccgcgttg ctatcgacga agacgcttcc ggtcatggat ttcatcgcgc gtgggaagct
      181 tttcgccggg ctgcctgaaa gcgaagggtt tccctgccat gaaccggcgg cgcgcggcct
      241 cataagcggc ctcataatcg gattcgtgcc tcatccgctc ttctagaagg tcccccaaga
      301 actgcgacag gcttttgccc tgctcggcgg cccttatgcg cggttctcgt tgaggctgac
      361 ggtgacattc ttcatttcac gaatctcgcg ttttcgtgtt ttcgcgtcaa tggatcagct
      421 tcagccactc gtcctccgtg agcacggtaa ggcccaaatc cttggcgtct ttcagcttcg
      481 agccggcgcc tgggccggca accacgtaat cggtcttctt cgagaccgag cccgccacct
      541 tggcgccgag gcgctcggcg cgggccttcg cctcagagcg ggtcatctgt tcgagcgtgc
      601 cggtgaacac cacggtcttg cccgagatgg gcgaggcgtg atcgaccgct tcgagcggtt
      661 caggagtcac ttccctcagc agctctttga cgacggtcac gttgtgcggc tcggcgaaga
      721 aatcggcgat cgccgccgcg accgtcgggc cgatgccctc gatgctgtcg agctcggcga
      781 aggcctcgct ctcgcgcccc ttggcggcgg cgcccatgct cgccatgaac cgttcgatcg
      841 tgccgtagtc ccgcgcgaga agccgcgccg tggtctcgcc gacgtgaggg atcccgagcg
      901 catagatgaa gcggtcgagc cggatgctgc ggcgcgcgtc gatcgcgtcg aacagcttct
      961 tcgccgaggt ctcgccccag ccttcgcggt cggcaagctt ggcgcttgcg cgcttgtcgc
     1021 gcgcgagaag cgtgaagatg tcgggcggcg actggatgag cccgtcgtca tagaacgact
     1081 tgatgtgctt ctccccgatc ccctcgatgt cgaaggcgtt gcgcgagacg aaatgcttga
     1141 gccgctcgac cctctgcgcc gggcaaatga gcccaccggt gcagcgcctg accgcgtcct
     1201 ccttgccggt gttgggattg atctcgcgcg aggcatggct gccgcaggcc gggcagaact
     1261 tgggaaagac gaatcgtttc gcgcccttag ggcgcttttc ttcaaccacg ccgagaatct
     1321 gcggaatgac gtcgccggcg cgctgcacaa tgacggtatc gcctttcctc acgtccttgc
     1381 gcgcgatctc atcctcgttg tggagggtgg cgttctgcac aacgaccccg ccgacggtga
     1441 cgggatcgag cttggcgact ggcgtcagcg ctcccgtgcg tcccacctgg atgtcgatct
     1501 cgcgcaggat cgtggtcgcc ttctcggctg gaaatttgtg ggcgatggcg tagcggggcg
     1561 agcgcgagac gaagcccagc cgctgctgca gatcgagccg gtcgagtttg tacacgatgc
     1621 cgtcgatatc gtagtcgagg cccgcccgcc cgctctcgat atcgcgatag gtttcgagca
     1681 tctcctcgac cgagcgggtg agcctgatca gcgggttcaa gggaaagccc caggcgcgca
     1741 tcagctgata gacgtcccat tgggtctcgg ccgaaaagcc gcccgccacc tccccccagc
     1801 cataggcaaa gaagcgcagg ggccgctttg ccgtgatcgc gggatcgagc tgccgcagag
     1861 accctgccgc ggcgttgcgc ggattggcga agaggcggtc gccaacgcgc gcctgctcct
     1921 cgttcaggcg ctggaacgcc gcgcggctca tatagatctc ccccctcagc tcgaacgggt
     1981 cgggaaagcc gcgcgatttt atcgtcttcg ggatgtcggc gatggtgcgg acattggcgg
     2041 tgacgttctc gccttcgtaa ccgtcgccgc gtgtcgcgcc ctggacgagc ttgccgcctt
     2101 cgtaacggag cgtgatggag agtccgtcga tcttgggctc cgcggtgaag gcaagcccct
     2161 cgatggtgtc gcgcccaaga aagcggcgga tgcggtcgag gaagtcgcgc aagcccgcct
     2221 cgtcgaacac gttgccgagc gacagcatgg gcatgcgatg ggtgaccttg ccgaacgcct
     2281 cgaccggggc cgcgcccacc ttgagcgagg ggctgtcgtc gcggacgaga tggggaaagc
     2341 gcgcttcgat ctcctggttg cgcgccctca agccatcgta ttcggcgtcg gagatctcag
     2401 gctgatcttt gcggtagtag agctcgtcgt gatgggcgat cgaagcggcg agccgggcaa
     2461 gctccgtcgc ggcctcggcc tcgtcgagct tggctgaagg cttgtcttcc agggcgggtc
     2521 cggctgcggc cgtctttctc ggcttcgtct tgctcattct ctctcatcag ccggtccggc
     2581 tcatgagccg ctcggccgcc ttgcgcgcct cttcggtaat ggtctgaccg gcgagcatgc
     2641 gggcgatttc ctcgcgccgc tgatcgcctt cgagaacggc aacacgggtt gccatggcct
     2701 cgcccgcttt cccgcgcacg ggctccttgg cgatcaggag atggccgctg gcaacggccg
     2761 caacttgcgg ggcatgggtc acggcaatga cctgcactct tgccgcaagc cgcgccaagc
     2821 gctc
//
LOCUS       DASUQP010000202         5229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_645773,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000202 DASUQP010000000
VERSION     DASUQP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645773"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="VFY74_08995"
     CDS             <1..1974
                     /locus_tag="VFY74_08995"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5931868.1"
                     /translation="KLGQALVPAAASGLPSIFLGEMPKPERKLPPVYLCETGPDGASC
                     RIKWLASTCLAGVVGVCLIGVAIYASLNMEDGSGMVSSIKRASLAALEPMRTATLAKS
                     WQSAGGHKEDRIHMTEAGFATRQVIHDTVIERHGSREFITIKPYLRIMAGLATEPPAD
                     ADELPPFNPFKLYSDSTPVGASGEAEDAPQAVTVNVVEVPGGILPQSDGVELKPEDVG
                     RLVAEAAEIFAYAEGATAGDGEARLIQAAYRPEEGLGASPQTTVIRKSAEDVAEEEED
                     TFDDLFAGAETKTLTVGRGDTLMSLFTKIDAEPSQAKEITETFEPIFAAKDLKSGQEV
                     RFTLVPAPSDSGQMEPAKVSIFAKGTHLATVVRNRQGLFVASAEAIGNDVKQQQAAAR
                     ATLYTSFYHAALAQKMPAETILKLLRVHSYDVDFKQRVKAGDSFDMFFDGGDEGELGE
                     LLYTSMTIDGAIRKFYRFRTPDDVVDYYDEQGNSAKKFLMRNPVKGGRYTSGFGDRRH
                     PLLRKIRMHTGVDWAAPSGTPILAAGDGTVERVGREGGYGNYVRIRHANGFATAYGHM
                     VRFAAGVEPGVAVKQGQIIGYVGSTGFSTGPHLHFEVLVNNSFVNPMTIQVPRGLQLT
                     GRQLAEFQRERRRIETLMQMDPVTSRVAQATTQ"
     gene            complement(2299..2925)
                     /locus_tag="VFY74_09000"
     CDS             complement(2299..2925)
                     /locus_tag="VFY74_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931869.1"
                     /translation="MPVILLAAGLAACVSGGGEDWRLHYTSFGANPPRGNRVTICHAY
                     TCKEQTPYTFNAAQVAEIAAIMKKVKRADTPFEERRGVAYAVSYMEVKVGNKLGIKDV
                     AGMQFTASGDKSQQDCVDEATNTTSYLLVLQENGLLKHHTVSGTMSKENLAKGIATLN
                     PVKYWPHWTAVLTETKTGQKYAVDSWLFPNGENPAVVKVEDWYIKDRE"
     gene            3630..4202
                     /locus_tag="VFY74_09005"
     CDS             3630..4202
                     /locus_tag="VFY74_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931870.1"
                     /translation="MRYLLIGNAPNCADAVVENAAHADVIIQCNGCTYAEVLPSVRDN
                     YGFVSNTAPKPEIIGAIEHRLTSLRSLPVFTSTSIILCRNPRAYTLGAYLLRGRLNVR
                     TEFFELNRFWPVRAVSFVSTVRLHTSLLKLGMQIRQMPSTGMVAYDWIRRRLRPTDSL
                     LVAGFTHQGWQGHPWHLEREIIKPVIGDRR"
     gene            4361..5098
                     /locus_tag="VFY74_09010"
     CDS             4361..5098
                     /locus_tag="VFY74_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931871.1"
                     /translation="MVSTLKNCFAQSKLAILGSGPSLSFFRGGYDCTIAVNGAALIDF
                     NYDYFMCGDFQSPQMPWFLASQRVNATRLIANFLAPFDPIVVPEKSDRDTMRNLPLFK
                     KKFSVWTRFSYFNFRPIERAYAPHRYFYYHPWSDTFLVRPVTSAFRGETPRFYCGATI
                     AGVAMQLASYMGAAEIHMYGVDMNNFDGKTYHDPAKNQGRIKRVQVRKLAWLTNKIQE
                     TGTKVVFHTASGHGLMPPEVQKQTALA"
     gene            complement(5095..5217)
                     /locus_tag="VFY74_09015"
     CDS             complement(5095..5217)
                     /locus_tag="VFY74_09015"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931872.1"
                     /translation="MQDPLAEMLLEGRIHDGESVKITAGKDGLMFNGEAAKQAA"
BASE COUNT          995 a         1667 c         1558 g         1009 t
ORIGIN      
        1 aagctcggcc aggccctcgt gccggccgcg gcttccggac tcccatccat ctttctcggc
       61 gagatgccaa agcccgagcg caagctgccg ccggtctatc tctgcgaaac gggccccgat
      121 ggagcaagct gccgcatcaa gtggctggcg agcacctgtc ttgccggcgt ggtcggcgtg
      181 tgcctcatcg gggtcgcgat ctacgcctcg ctcaatatgg aagacggctc cggcatggtc
      241 agctcgatca agcgcgcgag cctcgccgcg ctcgagccga tgcgcaccgc aacgctcgcc
      301 aaatcctggc agagcgccgg cggccacaag gaagaccgca ttcacatgac cgaagcgggc
      361 ttcgccaccc gccaagtcat ccacgacacc gtcatcgagc ggcacggcag ccgcgaattc
      421 atcaccatca agccttacct tcgcatcatg gccgggctcg ccaccgagcc gccggccgat
      481 gccgacgagc ttcccccctt caatccgttc aagctctatt ccgattcgac gccggtcggc
      541 gcgagcggcg aggccgagga cgcgccgcag gccgtgaccg tcaacgtcgt cgaggttccc
      601 ggcggcatcc tgccgcaatc ggatggggtc gagctcaagc ccgaggacgt gggtaggctc
      661 gtggcggagg ccgccgagat tttcgcctat gccgagggcg cgaccgccgg cgacggcgag
      721 gcccgcctca ttcaagcggc ctatcgtccc gaagaaggac tcggcgcttc gccgcagacc
      781 acggtgatcc gcaaatccgc ggaagacgtg gcggaagagg aagaggatac gttcgacgat
      841 ctcttcgccg gtgccgagac caagacgctg acggtcgggc gcggcgatac cttgatgagc
      901 ctgttcacca agatcgacgc cgaaccaagc caggcgaaag agatcaccga gacgttcgaa
      961 cccatcttcg cggccaagga tctcaagtcc ggccaggagg tccgcttcac gctcgttccg
     1021 gcgccttcgg acagcgggca gatggaaccc gccaaggtct cgatcttcgc caagggcacc
     1081 cacctcgcca ccgtggtgcg caaccggcag ggtctgttcg tcgcatcggc cgaagcgatc
     1141 ggcaacgacg tcaagcagca gcaagccgcc gcccgcgcca cgctctatac gagtttctat
     1201 cacgccgctc tcgcgcagaa gatgcccgcc gaaaccatcc tcaagctgct ccgcgtccat
     1261 tcctacgacg tcgacttcaa gcagagggtc aaggccggcg acagcttcga catgttcttc
     1321 gacggcggcg acgaggggga gctcggcgag ctgctctaca cctccatgac catcgacgga
     1381 gcgatccgca aattctaccg cttccgtacg cccgacgacg tggtcgacta ttatgacgag
     1441 cagggcaaca gcgcgaagaa gttcctgatg cgcaatcccg tcaaaggcgg ccgctacacg
     1501 tcgggcttcg gcgatcgccg ccatccgctg ctgcggaaaa tccgcatgca taccggcgtc
     1561 gactgggccg cgccctcggg caccccgatc ctcgccgccg gcgacggcac ggtggagcgc
     1621 gtcggccgcg agggcgggta cggcaattat gtccgcatcc gccacgccaa cggtttcgcc
     1681 acggcctacg gccacatggt ccgcttcgcc gcgggcgtcg agcccggcgt tgccgtgaag
     1741 cagggccaga tcatcggcta tgtcggctcg accgggttct cgaccgggcc gcatcttcac
     1801 ttcgaggttc tggtcaataa cagcttcgtc aacccgatga cgattcaagt gccgcgcggg
     1861 ctgcagctca cagggcgcca gctcgccgag ttccagagag agcgccggcg catcgagacg
     1921 ctgatgcaga tggacccggt cacgagccgc gtcgcccagg ccaccacgca gtagctcttc
     1981 tcgtttctct cctatttttc cgcttcgctg aatcatcccc tccccttgat ggggaggagc
     2041 ggcgcgcgac ctttcgcgcg ccgggtgggg tgacggcgtt tcaacccgcg accgcttagc
     2101 cggttgaagt gcaagccccc accccgacgc cgccttcggc gacgtcggac ctccccacga
     2161 gggggaggtt cgaggagaag ctgtgcctct gtcttcgctc tccccttggg gagagcacgc
     2221 gcgacatagc gcgcggtgag gggtcaggcg ccagcccgtc cttaactcac ccccatccac
     2281 agcaagtgga gagggagatt attcgcggtc cttgatgtac cagtcctcga ccttgaccac
     2341 ggccgggttc tcgccgttcg ggaacagcca cgagtcgacc gcatatttct gcccggtctt
     2401 ggtctcggtc agcaccgcgg tccaatgcgg ccagtatttc accggattga gcgtggcgat
     2461 gcccttggcg aggttctcct tcgacatcgt gccggacacg gtgtgatgct tgagcagccc
     2521 gttctcctga agcacgagca gatagctcgt cgtgttggtc gcttcgtcga cgcaatcctg
     2581 ctggctcttg tcgccggagg cggtgaattg cattcccgcc acgtccttga tgccgagctt
     2641 gttgccgacc ttcacctcca tataggagac ggcgtaagcc acgccgcggc gctcctcgaa
     2701 cggcgtgtcg gcgcgcttca ccttcttcat gatcgccgcg atctcggcga cctgggcggc
     2761 gttgaaggtg tagggggtct gctctttgca ggtataggcg tggcagatgg tgacgcggtt
     2821 ccctcgcggg gggttcgcgc cgaagctcgt atagtggagg cgccaatcct cgccgccgcc
     2881 tgacacgcaa gcggcgagcc ccgcggcgag cagtatgaca ggcacgcggc taagcacgtt
     2941 cccgatcccc cgatttccgg cacttctcct caaccggagg agacaacaga acgcgacaag
     3001 aggcaagcgc ttgaggggcg taccgagcct tgttttcctt gcctcttttc cgtcgcaatt
     3061 cggcgccgcg cgatacctga gcgcctcccc ctcgcccctt gagggagagg gcttcccgcc
     3121 gcgatcttgc ggcgtagctc gcaagtcgca tggcgggaag ggtgaggggt cacggtcttt
     3181 caacgcgaga gcctttgtca cttgggcgct acgcccctac tcggcgcgca tgcgctcaag
     3241 tagcggagcg gtagcataac aatagagcat gcgcgccgac ctcttcacga ggggcggttg
     3301 gaaaagggaa tcgtcatcct gaggcggccg cgaagcgggc ctcgaaggac gcggtcccta
     3361 tcgtcatcct tcgaggcatg ctctcgcccg gccttcgccg gagcgaagtc ccggctttgc
     3421 gcaggcaggt cgcacctaag atgagaccat catgtcacgg agggcttatt gaatgtacga
     3481 tggtgcaata cgcagagtgc tgcaccgttg ctggagaaac gtgcgccgtt cgatcctgcg
     3541 gggtgttagg tagattgaaa ctccacccat cggagcgtct gacacttaag agtgaagctg
     3601 gatgcgatac gtctaaagag tgaggccgga tgcgatacct cttgatcggg aacgcgccga
     3661 actgtgccga tgctgtcgtc gagaacgccg cgcatgccga tgtcatcatc caatgcaacg
     3721 gttgtaccta cgcagaggtg ttgccgtctg tccgagacaa ttacggcttc gtgagcaaca
     3781 cggcacccaa accggaaatc atcggcgcga tagagcatcg cctgacgtcc ctgaggtcgc
     3841 tgccggtatt cacgtctacg agcatcatct tatgccgcaa tccgcgcgcc tacacgctcg
     3901 gtgcgtatct gttgagaggt cgtctcaatg tccgtacgga gttttttgag ttgaatcgtt
     3961 tctggcccgt tcgcgcagtt tcttttgtct cgacggtgag acttcatacg agcttactca
     4021 agctcggaat gcaaattcgt cagatgccga gcaccggcat ggtcgcttac gattggattc
     4081 gtcgtcgcct gcgaccgaca gactcactgc ttgtcgcggg atttacgcat caagggtggc
     4141 agggtcatcc gtggcatttg gagagagaga tcataaagcc cgtgatcggc gatcggcggt
     4201 agcaccggcg gcgacacatc aagggcacag cgatcatacg cgtgccctca gaccgcccgg
     4261 gcgacgacgc gccgctctgg cgccgaaacc gcggctagac taacattgca agtggaccac
     4321 tcgctgggcc gatcacggcc aatccggggg tacagcctca atggtctcta cgctgaaaaa
     4381 ctgcttcgct caatcgaagc tggccatttt aggcagcgga ccgtctctat cgttttttcg
     4441 gggcgggtac gactgcacga ttgcagtcaa tggggctgcc ctgatcgatt tcaactacga
     4501 ctatttcatg tgcggcgact ttcagtcacc gcaaatgccc tggtttctgg cttcccagcg
     4561 agtcaacgcg acgcgcttaa tcgccaactt tcttgctcca ttcgatccga tcgtggtgcc
     4621 cgaaaagagc gatagagata caatgcggaa tttgccgctc ttcaaaaaga aattctccgt
     4681 atggacccgc ttcagttatt tcaactttcg acctatcgag cgcgcgtacg cgccgcatcg
     4741 ttatttttac taccatccat ggtcagatac gttccttgta aggccggtta cctctgcatt
     4801 ccggggcgag actcccaggt tctactgtgg cgctacgatc gccggagtcg cgatgcaatt
     4861 ggcttcgtac atgggagccg ctgaaatcca tatgtacgga gtcgatatga acaatttcga
     4921 cgggaagacc taccatgatc ccgctaaaaa ccaggggcgc ataaagaggg tgcaggttcg
     4981 aaaactcgct tggctgacaa ataaaattca agagacaggc accaaggtgg tctttcatac
     5041 agctagcggc catggcctca tgcccccaga agtacaaaag caaactgcat tggcctaagc
     5101 cgcctgcttc gccgcctccc cgttgaacat cagcccgtcc ttgccggcgg tgatcttcac
     5161 gctctcgccg tcatggatcc tgccttcgag caacatctcg gcgagcgggt cctgcacgta
     5221 tttctggat
//
LOCUS       DASUQP010000203         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_236600,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000203 DASUQP010000000
VERSION     DASUQP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236600"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VFY74_09020"
     CDS             <1..1173
                     /locus_tag="VFY74_09020"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX5931873.1"
                     /translation="VPGYAAASWERGYGDFVMKPDLSTLRRIPWLPATALVLCDVLDH
                     HHHEPLAHSPRAILRKQIARLGDQKMSSRMASELEFYLFDETYESAQEKLWNNLKTAG
                     SYIQDYHIFQTTKEEDVMRAIRNGLQGAGIPVENSKGEWGPGQEEVNVRYADPLTMAD
                     WHVILKNGCKEIAYGLGKSVTFMAKWRYDLAGSSSHIHASLWDAAGKTPLFLDGKGEH
                     GMSGLMRQFVAGQLAHAREITWFLAPYVNSYKRFQAGTFAPTKTIWSLDNRTAGFRLC
                     GAESKGIRIECRIGGADLNPYLAFAALIAAGLAGIEQKLELEAPFVGDAYQGKALTEV
                     PKTLREATELMRNSKMLRAAFGDDVIDHYVHNAEWEQFEYDRRVTDWELKRGFERS"
     gene            1189..2571
                     /locus_tag="VFY74_09025"
     CDS             1189..2571
                     /locus_tag="VFY74_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5931874.1"
                     /translation="MSNSVKIISPIDGSVYAERPIAKDAAIDAALSAARTALPKWRAT
                     PIGERGRYMLAFLDALLSMNDDITTELAWQMGRPVRYGGEKGGAEERTRAMVALAEEA
                     LQPYYPPEKQGFRRYIAREPLGIVMVIAPWNYPYLTSINTIVPALLAGNAVILKHAAQ
                     TLLVGERFAEAFRRAGLPKGLFHNLVLSHEQTEKLIGSGRIDHINFTGSVAGGRAIER
                     AASGSFASLGLELGGKDPAYVRADAKLDHAIENLVDGTYYNSGQCCCGIERIYVDASV
                     YDDFVEGFAELTRKYVVGNPLEHATTLGPMARASFADTVRAQVAEAKRKGAKPLINMK
                     VEGDASGSPYLAPEVLIDVNHQMDVMREESFGPVVGIMKVHDDDEAVTLMNDSPYGLT
                     ASIWTRDIERAAELGSRIETGTVFMNRCDYLDPYLTWTGVKETGRGTALSRLGFEALT
                     QPKSFHLREV"
     gene            2594..>3662
                     /locus_tag="VFY74_09030"
     CDS             2594..>3662
                     /locus_tag="VFY74_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004690269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5931875.1"
                     /translation="MSTPTANWSYPTAVRFGTGRIKELADACRAASIARPLLVTDAGL
                     AKLPITERTLGLLKDAGLPVAMFSDVQSNPVEANVEAGIAAFRAGKHDGVVAFGGGSG
                     LDTGKVIAFAAGQTRPLWDFEDIGDWWTRADADKIAPIVAVPTTAGTGSEVGRAGVIT
                     QEATQTKKVIFHPKMMPRVVICDPELTAGMPPAITAGTGMDALAHCLEAYCAVGYHPM
                     ADGIAVEGMRLIFENLPKAVANGNDLEARAHMMSAAAMGAAAFQKGLGAIHALSHPVG
                     ALYHTHHGTTNAVFMPYVLVFNRSAIEQKIARLAGYLGIEGGFDGFLEAVLSLRKATG
                     VPNDLRGLKVDGSKAETIAEMA"
BASE COUNT          692 a         1180 c         1164 g          626 t
ORIGIN      
        1 gtgcccggat acgcggccgc gagctgggag agaggctacg gcgacttcgt catgaagccg
       61 gacctctcga cactgcggcg cattccctgg ctcccggcaa cggcgctcgt gctgtgcgac
      121 gtgctcgacc atcaccatca cgagccgctc gcgcatagtc cgcgcgccat cctgcgcaag
      181 caaattgcgc ggcttggcga ccagaagatg tcgtcacgca tggcttcgga gctcgagttc
      241 tatctgttcg acgagacgta cgagtcggcg caagagaaat tgtggaacaa tctgaagacc
      301 gccggctcct acattcagga ctaccacatc ttccagacca ccaaggaaga ggacgtgatg
      361 cgcgccatcc gcaacggctt gcagggcgcc ggcattccgg tcgagaactc caagggcgaa
      421 tggggaccgg ggcaggagga agtcaacgtc cgctacgccg atccgctcac catggccgac
      481 tggcacgtca ttttgaagaa cggctgcaag gagatcgcct atgggctcgg caaatcggtg
      541 acgttcatgg ccaaatggcg ctatgacctc gccggctcgt cgagccacat ccacgcctcg
      601 ctgtgggacg ccgccggcaa gacaccgctg ttcctcgacg ggaagggcga gcacggcatg
      661 tcggggttga tgcgccaatt cgtggcagga cagcttgcgc acgcgcgcga gatcacctgg
      721 ttcctcgcgc cctatgtcaa ttcctacaag cgcttccagg ccggcacctt cgcgcccacc
      781 aagaccatct ggtcgcttga caaccgcacg gccggttttc gcctgtgcgg ggcggaaagc
      841 aaaggcatcc gtatcgagtg ccgcatcggc ggcgccgacc tcaatccata tctcgccttt
      901 gccgcgctga tcgccgcggg acttgccggc atcgagcaga agctggagct cgaggccccc
      961 ttcgtcggcg acgcctacca gggcaaggcg ctgaccgagg tgccgaagac gctgcgcgaa
     1021 gcgacggaat tgatgcggaa ttcgaagatg ctgcgcgcgg ccttcggcga cgacgtcata
     1081 gaccactacg ttcataatgc ggagtgggag caattcgagt atgaccggcg cgtcaccgat
     1141 tgggaattga agcgcggttt cgagcggtcg tgagccgaga ggcgtaagat gtcaaacagc
     1201 gtaaagatca tctcccccat cgacggctcg gtttacgccg agcgccccat tgcgaaagac
     1261 gccgccatcg acgccgcgct gtctgccgcg cgcacggcac tgccgaaatg gcgggcaacc
     1321 ccgattgggg agcgcggccg ctacatgctc gcctttctcg atgcgcttct ttcgatgaat
     1381 gacgacatca ccacggaact tgcctggcag atgggccggc ccgtccgtta tggtggggag
     1441 aagggcggcg cggaggagcg cacgcgcgcc atggtggcgc tcgccgagga ggcgcttcag
     1501 ccctattacc cgcccgaaaa gcagggcttc aggcgctaca tcgcgcggga gccgctcggc
     1561 atcgtgatgg tgatcgcgcc gtggaactat ccctatctca catcgatcaa cacgatcgtg
     1621 ccggcgctcc ttgccggaaa tgccgtgatc ttgaagcacg cagcgcagac gctccttgtc
     1681 ggcgagcgct tcgccgaggc gttcaggcgc gcaggactgc ccaagggcct gttccacaat
     1741 ctcgtcctat cgcatgagca gaccgagaag ctcatcggct cgggccgcat cgaccatatc
     1801 aatttcaccg gctcggtcgc cggcggccgc gccatcgagc gcgccgcttc cggcagcttc
     1861 gcttcgcttg gcctggagct cggcggcaag gatccggctt acgtgcgcgc cgacgccaag
     1921 ctcgatcatg ccatcgagaa tctggtcgac ggcacgtact acaattccgg ccagtgctgt
     1981 tgcggcatcg agcgcatcta tgtcgatgcg tcggtttacg acgatttcgt cgagggcttc
     2041 gccgaactca cccgcaaata cgtggtgggc aatccgctcg agcacgcgac cacgctcggc
     2101 ccgatggcgc gcgcctcctt cgccgacacg gttcgcgccc aggtcgccga agccaagcgg
     2161 aagggcgcga aacctctcat caatatgaag gttgagggcg acgcgtcagg atcgccatat
     2221 cttgcgccgg aagtcctgat cgacgtcaat caccagatgg acgtgatgcg ggaggagagt
     2281 ttcgggcccg ttgtcggcat catgaaggtg cacgatgacg acgaggcggt cacgctgatg
     2341 aacgacagtc cctatggact gaccgcctcg atctggacgc gcgatatcga gcgggcggcg
     2401 gagctcggca gccggatcga gaccggcacg gtctttatga accggtgcga ctatctcgac
     2461 ccttatctca cctggaccgg cgtcaaggag accgggcgcg gcacggcgct gtcgagactc
     2521 ggcttcgagg cgctgactca gccgaagagc ttccacctcc gcgaagtctg acccaagcaa
     2581 ttcggaaccg catatgagca caccgaccgc caattggagc tatccgaccg ccgtccgttt
     2641 cggcacgggc cgcatcaagg aacttgccga tgcctgccgg gccgcgagta tcgcccgccc
     2701 gttgctggtg acggatgcgg gtctcgccaa gctcccgatc acggaacgaa cgctcggcct
     2761 tctgaaagac gcgggacttc ccgtcgccat gttttccgac gttcagtcga acccggtcga
     2821 agcgaatgtc gaagccggca ttgccgcctt ccgtgccgga aagcatgacg gcgtcgtagc
     2881 cttcggcggc ggctctggcc tcgacacggg caaggtcatc gcatttgccg ccggacagac
     2941 ccggccgctt tgggacttcg aggatatcgg cgattggtgg acgcgcgccg atgccgacaa
     3001 gatcgcgccg atcgtcgccg tgccgaccac ggcggggaca ggctccgaag tcggccgcgc
     3061 cggcgtgatt acacaagaag cgacgcagac gaagaaggtg atctttcacc ccaagatgat
     3121 gccgcgcgtc gtcatctgcg atccggagtt gaccgccggc atgccgcccg ccatcaccgc
     3181 ggggacgggc atggatgcgc tcgcccattg tctcgaagcc tattgcgccg tgggctatca
     3241 cccgatggcc gacggcatcg cggtcgaggg catgcggctc atcttcgaga atctgcccaa
     3301 ggccgtggcc aacggcaacg atctggaggc gcgcgcgcat atgatgagcg cggcggcgat
     3361 gggggccgcg gccttccaga aggggctcgg cgccatccac gcgctgtcgc atccggtcgg
     3421 cgcgctctac catacgcatc acggcacgac caacgccgtg ttcatgccct atgtgctcgt
     3481 gttcaaccgg tccgcgatcg agcagaagat cgcgcggctc gccggttatc tcggcatcga
     3541 gggcggattc gacgggttcc tcgaagccgt gctttccttg cgcaaagcca caggcgttcc
     3601 caacgacctg cgcggactga aagttgacgg cagcaaggcc gagacgatcg ccgagatggc
     3661 cg
//
LOCUS       DASUQP010000204         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_373214,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000204 DASUQP010000000
VERSION     DASUQP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373214"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..641)
                     /locus_tag="VFY74_09035"
     CDS             complement(162..641)
                     /locus_tag="VFY74_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX5931876.1"
                     /translation="MWTPSLSIVELIFRGVVVYVALFSLLRLIGKKHVGELSPFDLVV
                     LLIISETVSGSLVGDDHSLTAGLVSAATLVAVVQIVGYFTWRFRKAERLVEGVPRILV
                     RHGHVNDSVMAEEQITRAELIEALRREGHTSLTGIRFALLETDGSITLAARAQDKKE"
     gene            complement(694..1725)
                     /locus_tag="VFY74_09040"
     CDS             complement(694..1725)
                     /locus_tag="VFY74_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5931877.1"
                     /translation="MSRITRFAKDVVNGWLAADAFSLAATLAYYAIFSIAPLLLLSIH
                     LASLFLDRNVAVERLTNELIGVIGPTGSDAIRQMLEAAGTAQTESWSGLIGLGVLLFA
                     TTGFVGSLQDALDRIWEAPPRPGGFGAFIKGKIFTFTLILAAAFLLLISLVLSTVMTA
                     VTGAITDAFGLPDGVVAAVIATANFLLATLIFAAIFKYVPSVFVTWRAAVVAGLITAA
                     LFALGRLALAWYLGREAEASAYGAATSLVLLLVWVYYSAQILFFGAQFSQSLQTAWLG
                     RRPVAGPAPHRPPSQLSPDTDLANVAGALVLGFVLGALVTQRPRPRSTFARPRRAPDW
                     LAFVRSWLG"
     gene            1833..2078
                     /locus_tag="VFY74_09045"
     CDS             1833..2078
                     /locus_tag="VFY74_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931878.1"
                     /translation="MTFGDTAPRSGGRRPKSPEELSDQIEALRAELQTLTSTVTDVAG
                     KQLTRAQESLEQNIRSNPIAAVGIAAAIGFFYALIRR"
     gene            2111..>2389
                     /locus_tag="VFY74_09050"
     CDS             2111..>2389
                     /locus_tag="VFY74_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX5931879.1"
                     /translation="MPAAQRLPLETMPNDVTAEELDFVRIDARLVERAVAVLQALERR
                     GAKAVSAESCTGGLIATVLSEAPGAAEHFEGGFVVYTPAQKFAALGIPP"
BASE COUNT          490 a          731 c          779 g          389 t
ORIGIN      
        1 cgtgtcgctg tggatggatg tcacatttgc cgtgggcgtg gcattctggt tcgtgctcgg
       61 agtcttgatc tggaactggc cgatgatgat cgcaaatgtg gtgacgtttt tgctgacgct
      121 cgtcatcatc gccatgaagc tttatctcga atagtccgcc gttactcctt tttgtcctgc
      181 gctcgcgcgg cgagcgtgat ggaaccgtcc gtttcgagca aggcaaagcg aatgccggtc
      241 agtgaagtgt gaccctcgcg gcgcagcgct tcgatcagtt ccgcgcgcgt gatctgttcc
      301 tctgccatga cgctgtcatt cacgtgccca tggcgcacga ggatgcgggg aacgccttca
      361 accagccgct cggccttgcg gaagcgccag gtgaagtagc cgacgatctg gacgacggcc
      421 acgagcgtgg cggccgaaac gagtcctgcc gtcaaagaat gatcatcgcc gaccaaagag
      481 ccactcaccg tctcgctgat gatcaggagc acgacgaggt cgaacggact gagctcgccc
      541 acatgcttct tgccgatgag ccgcagaagc gaaaagaggg caacataaac gaccaccccg
      601 cgaaagatca actccacgat gctgagcgat ggtgtccaca tgcaagcttc tccatgttga
      661 tgtgccgaaa ggatgcgaca agaccttgcc cgatcacccc agccaagacc ggacgaacgc
      721 gagccaatca ggcgcccgcc gcggccgcgc gaaagtgctg cgaggtcgcg gcctttgcgt
      781 gacgagcgcg ccgagcacga agccgagcac cagcgcgccc gcgacatttg ccagatcagt
      841 gtcgggcgag agctgggatg gcgggcggtg aggcgcaggc cctgcaaccg ggcgccgccc
      901 cagccatgcg gtttgcaggc tttgggagaa ctgcgcgccg aagaacagga tttgcgcgga
      961 gtaatagacc cagaccagca ggagaacgag cgaggtcgcc gcgccgtagg ccgaggcttc
     1021 ggcttcgcgc ccgagatacc aggctagcgc caaccgtccc agtgcaaaga gcgccgccgt
     1081 gatgagaccg gcgacaacgg ccgcgcgcca cgtcacgaag acggacggga catatttgaa
     1141 gatggcggcg aagatcagcg ttgcgagcag aaaattcgcc gtggcgatga cagccgccac
     1201 aacgccatcg ggcaggccga atgcatcggt gatggcgccc gtcaccgccg tcatgacggt
     1261 ggacaacacg agcgagatca gcagcaagaa ggcggccgcg agaatgagag tgaacgtaaa
     1321 gatcttgcct ttgatgaacg ccccgaagcc gcccggccgt ggcggcgctt cccagattcg
     1381 atccagcgca tcctgaagcg agccgacgaa gccggtcgtg gcgaacagca ggacgcctaa
     1441 cccgatcaga cccgaccagc tttccgtctg cgccgtgccg gccgcctcga gcatctgacg
     1501 gatggcgtcc gatccggtcg gccctatcac gccgatcagc tcattggtca gccgttccac
     1561 cgccacgttg cggtcgagga atagcgacgc gaggtggatg gaaagcagca agaggggcgc
     1621 aatcgaaaag atcgcgtaat aggcaagcgt cgcggccagg ctgaaggcgt cggcagcaag
     1681 ccagccgttt acgacatcct tggcgaagcg ggttatccgg gacatgtgcg cgggcctttt
     1741 tcctgcaaac acaccaagct tgccgacagt tccgggcgtg gaacgccctg cgcggacacg
     1801 cgttgagcag gaaatgtcac aggagcggta acatgacttt tggcgatacg gcgccgcgct
     1861 cgggcggcag aaggcccaag agcccggagg aattaagcga ccagatcgag gctttgcggg
     1921 cggaactgca gactctgacc tcgacggtca ccgacgttgc cggcaagcag ctcacccggg
     1981 cgcaggagag tctcgaacag aacattcgca gcaatccgat cgcggcggtc ggcatcgccg
     2041 ctgcgatcgg gtttttctac gcgttgatcc ggcgctagcg cgcgtgtgcc gggcttaaga
     2101 acgcatccgc ttgcctgctg ctcagcgctt gcccctcgaa accatgccga atgacgtcac
     2161 ggccgaagag ctcgatttcg tgaggatcga cgcccgcctc gtggagcggg cggtcgcggt
     2221 gctgcaagcc ctcgagcgga gaggcgcgaa ggcggtctcg gcggaatcct gcaccggcgg
     2281 cttgatcgcg acggtcttga gcgaggcgcc cggcgccgcc gagcatttcg agggcggctt
     2341 tgtcgtctac acgcccgcgc agaaatttgc cgcgctgggg attccgccc
//
LOCUS       DASUQP010000205         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2009493, whole genome shotgun sequence.
ACCESSION   DASUQP010000205 DASUQP010000000
VERSION     DASUQP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009493"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFY74_09055"
     CDS             <1..1039
                     /locus_tag="VFY74_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HEX5931880.1"
                     /translation="TGAVSRHNFRRQASERRVPMTDFDLRPASMAPTISRQAFLRAAL
                     ALAASPLLRYVPAQAASGFEVNEVAPGIFVHQGRYELQSPENRGDMANASFVVGRDGV
                     AVIDTLGSAVAGRAFKAALRSVTDKSIRYVINTHMHPDHVFGNAAFKEDKPTFVGHYK
                     LARGLGSRAERYLAINKQMLGEEAFEGIEVILPTVGVEDSLDLDLGGRTLQLKAQPTA
                     HTDNDLTVTDTATDTLFLGDLLFSVHVPTLDGSITGWLALLEALSRQTPARVVPGHGP
                     HAMAMPEALQPEQKYLGAIAADVRKLIKDGKTLEDAIRTAGFSEREAWKLFDHYHVRN
                     VTAAFAELEWE"
     gene            1075..1893
                     /locus_tag="VFY74_09060"
     CDS             1075..1893
                     /locus_tag="VFY74_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HEX5931881.1"
                     /translation="MPLPLKTLGFLGGAAALAGLLVLAPAAKADVDVWPTLKKQAFGD
                     RAIAENDGAVVLEIPGTAEDAAIVPLTVRVPPHVTGDLKSLTLFIDKNPDPKVATLTF
                     GPAQGEGGERSFSTRVRIETFSHVRAVLETEDGSLHMASKFVAAAGGCGAMQAKDPDT
                     EMVGLGKMIVKTFPPALEQNPIWSAQVMIKHPNANGMQLDINTANIIPARFVKEVTVK
                     RDGELVFKMDSTFSISTNPNFRFTFGRGEKNELDVVVTDTDATEFAAQSQPSGS"
     gene            complement(2698..2955)
                     /locus_tag="VFY74_09065"
     CDS             complement(2698..2955)
                     /locus_tag="VFY74_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931882.1"
                     /translation="MGLGLPAAAAEEAAKPADAEFGGECVMGLAFGKDIKTDCSVSTV
                     YDGKTYCFGNDTAKQLFLKKPGEFLLKAHVYHSSRQPKQGQ"
BASE COUNT          600 a          990 c          989 g          601 t
ORIGIN      
        1 gactggggcc gtgagccgcc acaacttccg ccggcaagcc tccgagcgaa gggtgccaat
       61 gacggacttc gatctccgcc ccgcatcgat ggcgccaacg atctcgcgtc aagcgttctt
      121 gcgcgcggca cttgcgctcg cagcctcccc gctcctgcgc tatgtgccgg cgcaagcggc
      181 aagcgggttc gaggtcaacg aggtcgcgcc cggcatcttc gtgcatcaag ggcgctatga
      241 gctgcaatca ccggagaatc gcggcgacat ggccaatgcg agcttcgtgg tgggtcgcga
      301 tggagtcgcg gtcatcgaca cgctcgggag cgcggtcgcg gggcgcgcgt tcaaggccgc
      361 cctccgctct gtcaccgata agtccattcg ctacgtgatc aatacccaca tgcaccccga
      421 ccacgtgttc ggcaatgccg ccttcaagga ggacaagccg accttcgtcg gccactacaa
      481 gcttgcgcgc gggcttggct ctcgggccga acgctatctc gccatcaaca agcagatgct
      541 tggcgaggag gcattcgaag ggatcgaggt catcctgccc accgtcggcg tcgaggacag
      601 cctcgatctc gatctcggcg gccgcacgct tcagctcaag gcgcagccta cggcgcatac
      661 cgacaatgat ttgaccgtca ccgacacggc gaccgatacg ctctttctcg gcgatctgct
      721 cttttcggtc catgttccga cgctcgacgg atcgatcacg ggctggctcg cgctgctcga
      781 agcgctctcc cggcagaccc ccgcgcgggt cgttcccggc catggaccgc atgccatggc
      841 gatgcccgag gcgcttcagc cggagcagaa atatctcggc gccatcgccg ccgacgtcag
      901 gaaattgatc aaggacggca agaccttgga ggatgcgatc cgcacggccg gcttttccga
      961 gcgcgaagcc tggaagctct tcgaccacta tcacgtccgc aacgtcactg cggccttcgc
     1021 agagcttgag tgggagtgat atagttgacc cgaacagtgg ataaggacga agccatgccc
     1081 ctgcccctca agaccttggg ctttctcggc ggcgctgccg cgcttgcggg ccttcttgtt
     1141 ctcgcgcccg cggcgaaggc cgatgtggac gtatggccga cgctcaagaa gcaagccttc
     1201 ggcgatcgcg ccatcgccga aaacgatggc gccgtggtgc tcgagatccc cggcacggcc
     1261 gaggatgcgg cgatcgtgcc cctgaccgtg cgcgtgccgc cgcatgtgac gggcgacctc
     1321 aaatcgctga cgctgttcat cgacaagaac cccgatccca aggtggcgac gctcaccttc
     1381 gggccggcac agggcgaggg cggcgagcgg agcttctcga cccgcgtccg catcgagacg
     1441 ttctcccatg tccgcgccgt gctcgagacc gaagacggct cgctgcacat ggcgagcaag
     1501 ttcgtggccg ccgccggcgg ttgcggggcg atgcaggcca aggatcccga caccgagatg
     1561 gtcgggctcg gcaagatgat cgtgaagacc tttccgcccg cgctcgagca gaacccgatc
     1621 tggtcggctc aagtcatgat caagcacccg aacgcgaacg ggatgcagct cgacatcaat
     1681 accgccaaca tcatcccggc gcggttcgtc aaggaggtga cggtgaagcg cgacggcgaa
     1741 ctcgtgttca agatggatag caccttctcg atttcgacca acccgaattt ccgcttcacc
     1801 ttcgggcggg gggagaagaa cgaactcgac gtggtcgtga ccgataccga cgctacagag
     1861 ttcgcggcgc agtcgcagcc gagcgggtcc taagcgcgcc gcggcgagct tccccaggac
     1921 cttctttccg ccgcgggctg ggtcttcctt cccgggtcaa agacgccgtg tctccaaaaa
     1981 aaagcatcgg gttttgaggg tcgcatgaac ctggctggct caagatgcgt ccaagttcac
     2041 tgtgagccaa cagatggagg ttaccatggc acggacacta gcttgggcgg caatgagcgc
     2101 gatcacattg gtcgttctct ctcaggatgc ttacgcctcc tgcggaccgc gcgttacaat
     2161 cacgtccccc tcgttgagca ggactgtggc tataggaaat tggcaagcca gagtcgcgaa
     2221 cacgatgaac gacctgtact cagattggaa ttacgccaca aagaagagct tgaccccaaa
     2281 agggaaaagc atcgtggcaa gtggcatccc ttgtaacacc cgctaaagca ggatgccgca
     2341 gatggtgtgg ggcgctcgct tcggcgggcg ccctttttgc tttatggctg acaatcgtcg
     2401 attctgttga gggactttat tgtcaaggtg aacgtcggca gcaaggttgg gctcatgctc
     2461 ggttccacgg caagctggct gagtttgcac ccctcttctt ccagaagcgg agcggtctag
     2521 cgccctcggt aatgtattaa gtgttcgtca tggccgggct tgtcccggcc atccacgatt
     2581 gctcttattg gagttgtttt aaaacgtgga tgccgggcac aagcttgcgc ccgggctggc
     2641 gaagccggac ccgggtggcc gggcatgacg gtcgtgctgg ggtgagaggc ttgggggcta
     2701 ctgaccctgc ttcggctgcc tactcgaatg atagacatgc gccttgagca ggaactcgcc
     2761 gggcttcttg aggaagagct gcttggcagt gtcgttgccg aagcaatagg tcttgccgtc
     2821 atagacggtc gagaccgagc agtcggtctt gatgtccttg ccgaacgcaa gtcccatcac
     2881 gcactcgccg ccgaactcgg cgtcggccgg cttcgcggcc tcctcggctg cggcagccgg
     2941 aagaccgagg cccatgagaa gggccgccgc tagtgcgcac cgtttcgctc ttcgccgcat
     3001 ggctcacctg gttcttaacg ggtccggcgt cggaaaaacc gggtcaaaag gaaagtcggc
     3061 gacgatttcg aggatttcaa gtcgtgagca tgaatttcac ggcatcctcc ttagggtgtc
     3121 cctccccatc gcacgacagg cgcctgttga gggaggggac cggcttcgtt cagcttcgat
//
LOCUS       DASUQP010000206        36276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2011106, whole genome shotgun sequence.
ACCESSION   DASUQP010000206 DASUQP010000000
VERSION     DASUQP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 36276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36276
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011106"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..707
                     /gene="speD"
                     /locus_tag="VFY74_09070"
     CDS             171..707
                     /gene="speD"
                     /locus_tag="VFY74_09070"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX5931883.1"
                     /translation="MSSDALFQLGMDLDHGSSTAQTEEKLSRCLSPNGEGRKDFFCER
                     DGVRYAGTHLIIDLVRAERLDDLDHIEHTLRRCVEVAGATLLHIHLHHFTPNGGVSGV
                     AVLAESHISIHSWPEYGYAALDVFMCGQTDPRAAIDVLKEAFSPRRIVVKEHLRGKEI
                     SRWNAGSKRRSTEASVSI"
     gene            656..1510
                     /gene="speE"
                     /locus_tag="VFY74_09075"
     CDS             656..1510
                     /gene="speE"
                     /locus_tag="VFY74_09075"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX5931884.1"
                     /translation="MERWVEETLHRGFRVNLKADRVLFDSETEHQRLIIFDNADFGRV
                     MMLDSIVQVTTKDEFIYHEMMAHMPLFAHGRAKRALIVGGGDGGVLREALRHPALEQV
                     TLCEIDRGVIDLCRQYFPEISAGAYDDPRARIVIADGTKFVAETADRFDVIMVDSTDP
                     VGPGAVLFTRAFYAGCRRCLKPGGLLVTQNGLPFLQAGELEQSVGYFRELFADAFAYL
                     ATTPTYFGGPMSYGWATDDKTLREHKRKKIARRHEKAGAFATRYWTPEVHVASFALPS
                     YVQELVAA"
     gene            complement(1507..1869)
                     /locus_tag="VFY74_09080"
     CDS             complement(1507..1869)
                     /locus_tag="VFY74_09080"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX5931885.1"
                     /translation="MTPQEVRLWVRLRSLRVLGHHFRRQSPITPYVVDFECRRSRLII
                     EVDGGQHACDGDQRRDVARDHHLQKSGYQVLRFWNNQIDREIDGVLEMILLALEPPPP
                     HPASPKRRKPPSPRGEGG"
     gene            complement(1971..3227)
                     /locus_tag="VFY74_09085"
     CDS             complement(1971..3227)
                     /locus_tag="VFY74_09085"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEX5931886.1"
                     /translation="MPDLVSGNAAIGQSGGPTAVVNQSLAGVIEGLRAGLAASGKVGR
                     IFGMRHGVRGLIKPSADGLLDLTALDKAKLEAIARTPSAALGSTRDKPDEAYCEKILA
                     SCRKNDIRYFYYIGGNDSADTCRIVSEKALAAGYELRCFHVPKTIDNDLLENDHTPGF
                     PSAARYVALALMGDALDNASLGGIKINVIMGRNAGFLTAAAALARSAAPDGALPEHRP
                     APHLIYLPEVPFSTDAFLADVEAVYAKLGRCQIAVSEGIRDAQGAEIGPKLMRGPGEV
                     DAHGNVQLSGSGALGDGLADLVKTALTPKGGKAPRVRADTFGYIQRCWPEPSPVDTLE
                     ARAAGRYAAELAARGDASASVIIVRTSNAPDAYRSACARADLAAVARRTRHVPAEFIS
                     GANDVSAAFHAYCRPLVGDLPRMERL"
     gene            3514..4464
                     /locus_tag="VFY74_09090"
     CDS             3514..4464
                     /locus_tag="VFY74_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX5931887.1"
                     /translation="MSSRDVWPELPYEAWKETCATLQLWTQIAGKIRLKQTPWLNHSW
                     HVVLYVSSRGLTTSPIPFGDRTFQLDFDFLDHMLWVSTSDGGRRRVGLYPRSVADFYA
                     ELMRSLTDLGINIRINELPNEIPDAIRFSEDHVHASYDGEFAQRFWRILAQSTQVMSR
                     FRTSFMGKCSPVHFFWGSFDLAVTRFSGRPASPLPSGSGSVPNMPASVVLDAYSHECS
                     SAGFWPGGQGVDYAAFYSYAAPSPPGFDKATVRPAEAFWNGDLNQYLLPYEAVRTASD
                     PEETLMAFLTSTYEAAATAGNWDRAALESPLGVPGVPRKV"
     gene            complement(4720..5715)
                     /gene="egtD"
                     /locus_tag="VFY74_09095"
     CDS             complement(4720..5715)
                     /gene="egtD"
                     /locus_tag="VFY74_09095"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX5931888.1"
                     /translation="MGSDISLHASAGSAARSGARPEEAAEDDFAAAVLAGLSRKPRSI
                     PCRFFYDARGSELFEEITKLEEYYPTRTETALLEAYGSEIAALAGEGRVLVEFGSGSS
                     RKTSLLLSALRGVPAYIPIDIAAESLEEAAEWLSGQHPGLTILPLIADFTSTRALPQA
                     ARSKKRLGFFSGSTIGNLSHDEAKAFLANAARLLGRGSVFLVGLDLKKPASILVPAYN
                     DASGVTAAFNLNLLARINRELKGTFDLSRFAHDAVWNAKAGRMEMYLKSLADQTVRVL
                     GRGFGFAKGERIHTENSHKYTVDEFQALASAGGWRPVKAWTDTDILFSLHLLRLE"
     gene            complement(5732..7018)
                     /gene="egtB"
                     /locus_tag="VFY74_09100"
     CDS             complement(5732..7018)
                     /gene="egtB"
                     /locus_tag="VFY74_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX5931889.1"
                     /translation="MNLHSAVLSTAEKPKTESPVLRDRLFATRALSSELTSPLSDEDQ
                     VVQAMDDASPAKWHLAHTTWFFEAFLLKRFLAGYRVFDERFEYCFNSYYESVGPRHPR
                     PKRGLLTRPAAEEVRAYRASVDAALDRLFDGPLAPDAAALIELGINHEQQHQELLLTD
                     ILSLFAAEPLKPAYREVDPGVPAGETEPISWLSFDGGIVEVGHEGEGFAYDNEGPRHE
                     TLIRPFKLADRCVTNSEWSEFIADAGYATPTLWLADGWNVVRGQDWRAPLYWEDDEGG
                     AAQMSLNGFRPIDPAAPVTHIGYYEADAFARWAGYRLPTEFEWEVAAASAPLEGRTLR
                     SGSLQPMAASTGEGLKQMIGDVWEWTGSAYLPYPGFKAAPGAVGEYNGKFMCNQFVLR
                     GGSCVTPEGHIRRTYRNFFYPHQRWQFTGLRLAQDG"
     gene            7180..7665
                     /locus_tag="VFY74_09105"
     CDS             7180..7665
                     /locus_tag="VFY74_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEX5931890.1"
                     /translation="MQDTRARDDAIIPFDGRQSPVAAALQRGVCRALRARGHSVVTEL
                     CLANGRRADVVALSGAGDITIVEIKSCLIDYRSDGKWQDYGPYCDRLYFAVAADFPRE
                     IIPPPVGLILADRYGAELVREPVEERLSAARRKAMMLAFARAAALRLQLHLDPSCGFE
                     A"
     gene            complement(7947..8525)
                     /locus_tag="VFY74_09110"
     CDS             complement(7947..8525)
                     /locus_tag="VFY74_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEX5931891.1"
                     /translation="MEADQALPSTEAHSGKLADNTLLIVDDDRAFLARLARAMEARGF
                     EVEAAASVEDGLSSLKRRPPAFAVVDMRLEDGNGIDVIAALKEVRPEARAVVLTGYGN
                     IATAVTAVKLGAVDYLAKPADADDVYNALVASASTVKAAPPENPMSADRVRWEHIQRV
                     YELCDRNVSETARRLNMHRRTLQRILAKRAPK"
     gene            complement(8706..10103)
                     /locus_tag="VFY74_09115"
     CDS             complement(8706..10103)
                     /locus_tag="VFY74_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEX5931892.1"
                     /translation="MNAVDNSQTIRRATVPAWLRQGETRLRLQTIVRLRWLAVLGQSI
                     TVIGAYWGLGIDLPIAGCLAVIALSASLNVALRLRYPASKRLKSSFAFAMLGYDILQL
                     AVLLYLTGGLQNPFAFLLIAPVTVSASTLPLGITAALGALAILAASVLTTFHHPLPWF
                     NHTNLLLPFPYVMGAWAAVVSGILFIAFYSWRTAEEARRMARALAATEMVLAREQKLS
                     ALDGLAAAAAHGLGTPLATIAVVTKELIRGAGPDDPHYDDLSLLRGQAERCRDILAEI
                     GGGGEQRDLIVSRLSLSHLIEEVVAPHRVMAVPIEVRIGPAQDGGSHGVTLAEPVSER
                     NPGVLYGLGNIVENAVDFAASKVNVEARWSSDEVRIVIADDGTGFPPNVLEQLGEPFV
                     TTRATRTDGPDTPDEHVGMGLGFFIAKTLLERSGARLELANRGGAVVTVMWPRARFEQ
                     APGEAGARAEPAAFA"
     gene            10168..11022
                     /locus_tag="VFY74_09120"
     CDS             10168..11022
                     /locus_tag="VFY74_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5931893.1"
                     /translation="MNDRVSKVGRDIGAPAAAGKGPDGKDVPAKVRDLTKIYGAVTAV
                     DRISFTLAEGTTVALLGGNGAGKTTTIAMLLGLVVPSAGEVRVFGEDVSRNRGAVAHR
                     MNFQSPYVDLPARLSVQQNLTVYAGLYGIANAVERVAYIAEELQIGPLLDRPTGKLSA
                     GQKTRVGLAKALLNAPDLLLLDEPTASLDPDTADWVRRKLKDYADTRGATLLLASHNM
                     AEVERLADRVILLDQGRIIEDETPADLIATYGRETLEEVFLDVVRGRAKQDPDAVDRG
                     AAPERKAS"
     gene            11058..11876
                     /locus_tag="VFY74_09125"
     CDS             11058..11876
                     /locus_tag="VFY74_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5931894.1"
                     /translation="MILPPTGARASLRRIGALLERYIYLLRSSGIRLLELIYWPFLQM
                     LTWGFLQTYLAETQSPVAQAAGVLIGSVLLWDILFRSKIGFSTTFIEEMWARNLGNLL
                     ISPLRASELVAALSLWSVIRLSVSMIPVAFAAYLIFGFNLLELGFALAAFFAVLVLTS
                     WSLGLISAGVILRYGLGAEELAWSLAFLLLPICCVYYPVSVLPEWLQPVALALPPTHV
                     FEGMRAILLEHRFDANDLWWALGLNAVYLIVGYLVFIRFLASARVNGSLLQLGE"
     gene            11877..12479
                     /locus_tag="VFY74_09130"
     CDS             11877..12479
                     /locus_tag="VFY74_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5931895.1"
                     /translation="MRTRLIVVIVAGFLIGAIAGAAVLALTGNGSGPQVATSGKALIG
                     GPFALIDQNGKTVTDQNFRGRFMLVFFGFTHCPDICPAELQVISQSLEALGPQAEEVV
                     PIFITLDPERDTQAVMDAYVKNFGSRFVGLTGSPEAVATAAKGYRIAYSKFQEDPASS
                     DYSVDHSALVYLMGRNGEYVTHFAYGTPADKMTETLRRYL"
     gene            12591..13862
                     /gene="phaZ"
                     /locus_tag="VFY74_09135"
     CDS             12591..13862
                     /gene="phaZ"
                     /locus_tag="VFY74_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX5931896.1"
                     /translation="MLYHFYELNRAALSPARAAADAYRLLFRNPLNPAYYTPLGRSAA
                     AALELFERTTRRYRKPSWGIDKTKVRGREVAVSERVVISKPFCNLVHFEREGSRWRLR
                     NDPKLLVVAPMAGHFATLLRGTVRDLLPYYDVYVTDWQDARFVPRAAGPFDLDVYIDY
                     IIEFLRYFRGDVHVMAVCQPAVPVFAAVALLEGMGDRSSPRSMILMAGPIDARENPTA
                     VNRFAEARTIDWFRRNVIAQVPWPYPGVMRMVYPGFLQLSGFMGMNFGRHVSAHRDLF
                     HNLIKGDGDSADKHREFYDEFLAVMDLAAEYYLQTIETVFLEHTLAKGQMTHRNRLVE
                     PSAIRKVALMTVEGEKDDITGMGQTFAAQTLCTNLPNTMRAHHLQSGVGHYGVFNGSR
                     FRAEIVPKIRDFIATHQRRGGLLARAFGRAA"
     gene            13947..14786
                     /locus_tag="VFY74_09140"
     CDS             13947..14786
                     /locus_tag="VFY74_09140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX5931897.1"
                     /translation="MLKRLNLARGETIEVKIRGLDIPMVLRRSARARRFSLQVNEARR
                     GAVLTMPLYSGFADADEFLSRHLDWLKERVAGLSAPVPFTHGAIVPLRGRAHVLRFAG
                     LVRRRGVVWIEAAEDAKAAPAWPEGARVGVRRLPRLYVAGADEHAPRRLLDWLKREAH
                     LDLKARVEHHARRLNLAPKRLFVRDQTTRWGSCSTSGALSFSWRLVLAPPFVLDYLAA
                     HEVAHLAHMNHGPRFWALVERTMPRHQEARSWLRKHGASLHRCGAEGVKPSSRTSPKR
                     SIE"
     gene            14793..15445
                     /locus_tag="VFY74_09145"
                     /pseudo
     CDS             14793..15445
                     /locus_tag="VFY74_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711861.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(15510..17543)
                     /locus_tag="VFY74_09150"
     CDS             complement(15510..17543)
                     /locus_tag="VFY74_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX5931898.1"
                     /translation="MQGARHTARPGAKVSRGPQHSSGARRGSANPRPGSARRRPSRLK
                     RWARRHPWLARALRYAAVASLCGAAVIGVMAIYMIAQMPDPRSAALDDRPPNVTVLAQ
                     DGAVIAERGLRRGHMRLDALPPYLVQAVLATEDRRFYAHFGVDPLGLIRASLRNASSG
                     AVVEGGSTITQQLAKNLFLTPKRTLSRKLEEAVYALWLEQRFSKDEILELYLNRVYFG
                     GGTYGVEAASRRYFGKSSRFLTLPQAALLAGLLKAPSRYAPTRSAKRAGIRVDEVLEN
                     MVEAGYLTEEQARNANEQPLKLSAKDDDTGYAYAVDWVAELLPELMGEQHGDLFVETT
                     IDAGLQRASQQALRRILDSEGTAVAASEGAVVVLDPSGEVKALVGGRAYSTSPFDRAL
                     KARRQPGSAFKPFVYLAALEAGYSPDSLAHDGPITVAGWSPRNYTGKYRGEISLRDSL
                     AHSINTVAVRITAKVGPSQVARTAERLGIHSELHDRPSIGLGTAEVTLLELAGAYAPF
                     ANGGHGVLPHIITRVRDGDGKVLYQRRRSTIGQVVALNDVGAMNDMLNATIRFGTGQK
                     AAIEGHEAAGKTGTTQSFRDAWFVGYTAHFVGGVWIGNDNGRRMKKVTGGTLPATLWH
                     DVMLYAHQEKPPLPLPGSNGPRLPEAMAALPWQSSSGGDTPFLRRVFGFFGGR"
     gene            17773..18063
                     /locus_tag="VFY74_09155"
     CDS             17773..18063
                     /locus_tag="VFY74_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5931899.1"
                     /translation="MRGGWVYILTNRRNGILYTGVARDLARRIWQHREGLGGVFTRRY
                     GLKRLVWCEYHDTILGAIQREKNLKHWPRAWKVRLIHAANPDWDDLYERLNQ"
     gene            complement(18320..20752)
                     /gene="gyrB"
                     /locus_tag="VFY74_09160"
     CDS             complement(18320..20752)
                     /gene="gyrB"
                     /locus_tag="VFY74_09160"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX5931900.1"
                     /translation="MTPASKRKEAAASEYGAESIKVLKGLDAVRKRPGMYIGDTDDGS
                     GLHHMVYEVVDNAIDEALAGFSDEIAVILNADGSVTVEDNGRGIPTEIHKGEGVSAAE
                     VIMTQLHAGGKFDQNAYKVSGGLHGVGVSVVNALSSRLDLTIWRDGKEHFMRFADGVA
                     EAPLKPVADAGAKRGTRITFVPSTKTFTMVEFDYSTLEHRLRELAFLNSGVRIVLSDN
                     RGVEPKVEEMRYEGGLRAFVRFLDRTKHPLIQEPIVVAKEQQKILVEAALWWNDSYHE
                     NVLCFTNNIPQRDGGTHLAGFRAALTRTVNTYDAESGIAKKEKVALTGDDAREGLTCV
                     LSVKVPDPKFSSQTKDKLVSSEVRPVVESVMAAALSGWFEENPGEARNIVQKIVEAAA
                     AREAARKARELTRRKGALDLASLPGKLADCQERDPAKSELFLVEGDSAGGSAKQARDR
                     AFQAVLPLRGKILNVERARFDKMLSSAEIGTLITALGTGIGHDEFDLAKLRYHKVIIM
                     TDADVDGAHIRTLLLTFFYRQMQSLVEAGHLFIAQPPLYKVKRGQSERYLKDEKALED
                     YLIGEGLEDAVLVTGSATRASADLRDIVETARRIGVILKGLHSRYPRFIVEQAAIAGV
                     LNPKVLKDIKQAQQAAAYIAKRLDALAEETERGWRGEALPEGGLRFWRELRGVMETHL
                     IDGPFIASADALKLDGFAEHLQEVYARPATLKRKDTSLAVHGPSELLDAVMTAGRKGL
                     TLQRYKGLGEMNPDQLWDTTLDLDTRTILQVKIKEGEDADDIFSRLMGDVVEPRREFI
                     QANALQVANLDV"
     gene            complement(20809..22002)
                     /gene="recF"
                     /locus_tag="VFY74_09165"
     CDS             complement(20809..22002)
                     /gene="recF"
                     /locus_tag="VFY74_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX5931901.1"
                     /translation="MRADRKGEGAAEAIAGDPAKTLFFVRSLRVADFRNYERAELDLD
                     ARPVVLFGENGAGKTNLLEAVSLLGPGHGLRGRPYVELARKEGAGGFAVAARVVSGED
                     EVEIGTGLAAGENEDATGRTVRIAGKERSAGALAEYVNLVWLIPAMDGLFTGPASERR
                     RFLDRLVVAVDPKMRSPLGRYDRAMRQRNRLFQMREGSRALFEGLEEQMAEAGVAIAA
                     ARLDATGRLVTLIERMRAERGEGPFPWASVALEGRLEAALATHAATEVEDEFRASLEE
                     GRERDRAAARALEGPHLSDLIVTHGPKEAPATECSSGEQKALLIGLILAKAELIKEIE
                     GVAPLVLLDEVAAHLDEARREALFGQILALGCQAWITGTDRDLFAPLAQDAQIFAVNR
                     GMIGA"
     gene            complement(21999..23117)
                     /gene="dnaN"
                     /locus_tag="VFY74_09170"
     CDS             complement(21999..23117)
                     /gene="dnaN"
                     /locus_tag="VFY74_09170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5931902.1"
                     /translation="MRLTIDKPGLAKALAHVASVVERRNTIPILSNVLLSAGRNELKL
                     TATDLDMELVETVPVRAKGEGGTTVPAHMFYDIVRKLPEGAEIELARDGEQGRLSIHS
                     GPAQFSLQTLPADDFPALSVDDLGRSFSLPASELKRLIDKTRFAISTEETRYYLNGIY
                     LHAAQSGGKPVLRAVATDGHRLAQIELPLPPGAKDMPGVIVPRKAVLELAKLVEDAEG
                     EVRVELSPSKIRVSTARVVLTSKLIDGTFPDYERVIPQSNDKTMEVDNAAFAQAVDRV
                     STLSSDKGRAVKLTINEGKLTLSVNNPDSGSATEELSVEYRFDPLEIGFNARYLLDIS
                     AQLESGTAEFQLADPGSPTMVRDGKDASALYVLMPMRV"
     gene            complement(23317..24744)
                     /gene="dnaA"
                     /locus_tag="VFY74_09175"
     CDS             complement(23317..24744)
                     /gene="dnaA"
                     /locus_tag="VFY74_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5931903.1"
                     /translation="MAQQEQGAPSDAWARVKSRLRAELGEDVFASWFRGVEVEQVDGE
                     VVHLTVATRFLRNWLRSHYYDFVLRLSRAEWPEVERVEFRVRQPHFGVEAPKEQPRLR
                     RSPEPPVPEALSPLPLPLRTGYGGFEGSPLDPRLSFVSFMVGTSNRLAYAAAQEVAGA
                     FSAPQPLFNPLFVHGNVGVGKTHLLHAIAWDVKKRKPESQVLYLTAERFMSGFVQALR
                     ARDALAFKEKLRRIDILLIDDMEFLQGPTIQQEFCHTLNSLIDGGRQVVVAADRAPTQ
                     LDKLDMRMRSRLGGGLVAEIGAMDYDLRHKILEKRAAEACAETKGLSISDTVLAFLSE
                     RLTESGRELEGAVHRLRASFQLTDQPVTLETAEQIVRDLMRGAEPRRVRIDDILRTVS
                     KHYGVNRGDLLSGRRNRSIVRPRQIGMYLAKLLTSRSLPEIGRRFGNRDHTTVLHAIR
                     KIEQLMNDDTQLREEIELLKRLLRE"
     gene            complement(24864..25310)
                     /locus_tag="VFY74_09180"
     CDS             complement(24864..25310)
                     /locus_tag="VFY74_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5931904.1"
                     /translation="MSTQNNETAVDDVDVETVWTTLQSHPRSQLVDVRTRAEWTYVGI
                     PDLAPVGKRPVLVEWQTFPDQAIDTRFAERLAGELKALGVGLGDELFFICRSGSRSLA
                     AAKAMAAIGYRACHNVACGFEGPLDDGRHRGAIAGWKAAGLPWQQS"
     gene            complement(25880..26149)
                     /gene="rpsT"
                     /locus_tag="VFY74_09185"
     CDS             complement(25880..26149)
                     /gene="rpsT"
                     /locus_tag="VFY74_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5931905.1"
                     /translation="MANTKSAKKAARQATRRTAVNKTRRTRLRSSMRNVEEAIASGNK
                     EAAAAALKEAEPIIARGAQKGVAHRKAASRKVSRLAKRVGAMGTR"
     gene            26360..27367
                     /locus_tag="VFY74_09190"
     CDS             26360..27367
                     /locus_tag="VFY74_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX5931906.1"
                     /translation="MRVLVTGAAGFIGYYTAARLLDRGHEVIGFDNLSPYYDVSLKEA
                     RLRRLNEHEGFRFLKGDLADRDVVEATFAEERPERVVHLAAQAGVRYSLDHPHAYISS
                     NLTGFLHVLEGCRHHGVEHLLYASTSAVYGANQTLPFAVCDNVDHPVSLYGATKKANE
                     LMAHSYAHLFGIPVTGLRFFTVYGPWGRPDMSLFLFTRNILAGEPIQVFNYGQHSRDF
                     TYIDDAVEGVLRTLDVVATPDPNWRAAAPDPATSSAPYRLYNIGSHNPVALLDYVAAI
                     EKALGRKATIELLPQQPGDVVATYADVEALQEATGFQPSTSLEEGIGRFVAWYRDYYR
                     V"
     gene            complement(27405..28352)
                     /gene="mutM"
                     /locus_tag="VFY74_09195"
     CDS             complement(27405..28352)
                     /gene="mutM"
                     /locus_tag="VFY74_09195"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX5931907.1"
                     /translation="MPELPEVETVRRGLAPFLVGNAFARVEQRRPDLRFPLPNAFAQR
                     LEGRKVEALGRRAKYLLARLDNGEVLVMHLGMTGRFSIDCPPRKKHPSRDGTDISRGV
                     SRGFEDSPLVLGEFAYTEERTPKHEHVVFHLVDGTALRYHDARRFGFMDLVPEAALDD
                     HALFRGLGVEPLSPPFTPEWMAQRLKGKTTSIKAALVDQTLIAGLGNIYACEALHRAG
                     ISPLRKAGSLATKAGKPTKKTEALVASVRAVLEAAIAAGGSSLRDYKRADGRLGRFQH
                     RFRTYDREGKPCPRKGCGGTIRRIVQGGRSSFYCPTCQR"
     gene            28441..29211
                     /gene="ubiE"
                     /locus_tag="VFY74_09200"
     CDS             28441..29211
                     /gene="ubiE"
                     /locus_tag="VFY74_09200"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX5931908.1"
                     /translation="MSEDLKGRAPDNAKTSFGFARVGEGAKQGLVNEVFARVAPRYDV
                     MNDLMSGGLHRLWKDDLVSWLAPPRSAAPYVLLDVAGGTGDVALRALHASGPGASAVI
                     CDISSEMIEAGRRRAGGERLSFVRGNAEALPFAKGRFDAYTVAFGMRNLTHLEAALAE
                     AFRVLKPGGRFLCLEFSAVEVPLLDRLYEAYSFNAIPALGRLVAGDSASYRYLVESIR
                     RFPDQENFTHLIENAGFERVRYRNLSGGIAAIHSAWRL"
     gene            29243..30805
                     /gene="ubiB"
                     /locus_tag="VFY74_09205"
     CDS             29243..30805
                     /gene="ubiB"
                     /locus_tag="VFY74_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEX5931909.1"
                     /translation="MARLTKAGAVLAWSGARVLPEQANLPAPLALFARVTAPLRKNGP
                     GEGRNETKLSDALTSLGPSYIKLGQFLATRDDIIGRELARDLSTLQDRLPPFSQEEAR
                     EAVKEALGAPIDKLYAEFGPPVAAASIAQVHKAKVRGADGTLTPVAVKVLRPGIEQRF
                     QKDLDSYFFAARMIERLHPPSRRLRPIAVVDTLAKSVAIEMDLRMEAAAISEMAQNIK
                     NDEGFRVPTVDWTRSARRVLTLEWIDGISIADREGLAAAGHDIKGLGPRVMQAFLRHA
                     MRDGFFHADMHPGNLFVDAEGRIVAVDFGIMGRLGLKERRFLAEILYGFLTRNYTRVA
                     EVHFKAGYVPRKHSVAQFAQALRAIGEPIMDRPANEISMAQLLGQLFQYTEVFDMQTR
                     PELLLLQKTMVVVEGVGRSLDPELNIWIVSEPVVKAWMEQEFGTGAQFEAAAQGAASV
                     GRLMGDIPRLLGQAERTAEALSAMAEEGVRLDDHTVERLAEAQGRENRASRIGIWIGA
                     LALIAIALSLLF"
     gene            31229..32473
                     /gene="coaBC"
                     /locus_tag="VFY74_09210"
     CDS             31229..32473
                     /gene="coaBC"
                     /locus_tag="VFY74_09210"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5931910.1"
                     /translation="MNGKRILLIVGGGIAAYKSLDLVRRLKERGAAVRAILTRGGAEF
                     VTPLSLAVLTEEKTFTDLFDLKDEAEIGHIGLSREADLVVVAPATADLLAKMANGLAD
                     DLASTVLLATDKKVLAAPAMNVRMWHHPATQRNLAILRADGIGFVGPDEGEMACGEYG
                     LGRMAEPQAILAAIEEMLGAEALAPRADATPLRSLAGCRVLITSGPTQEPIDPVRYIA
                     NHSSGKQGHALARAAIALGAEVTLVTGPVALPDPQGARVVKVKTAEEMLAAVLAALPA
                     DIAICAAAIADWRAASEARQKLKKEGGEASLRLMQNPDILATIARPGPKRPRLVIGFA
                     AETENLIANASEKRRAKNADWIVANDVSSGRGVMGADQTHVHLITASGVEDWPSLSKD
                     ETATRLLARAANALAAMRPAAE"
     gene            32470..32919
                     /gene="dut"
                     /locus_tag="VFY74_09215"
     CDS             32470..32919
                     /gene="dut"
                     /locus_tag="VFY74_09215"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX5931911.1"
                     /translation="MSVTVRVKRLPHGEGLPLPSYQSGDAAGLDVVAGVPEGSPLVLL
                     AGARALIPTGFALELPRGYEAQVRPRSGLALKHGVTLLNGPGTIDSDYRGELMVILIN
                     HGAEPFVLRRGDRIAQLVIAPVARVEIEGADALGTTERGQGGFGSTG"
     gene            33172..33999
                     /locus_tag="VFY74_09220"
     CDS             33172..33999
                     /locus_tag="VFY74_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX5931912.1"
                     /translation="MMLTPDEIERYKRQLVLKEIGGEGQQKLKASRVLVVGAGGLGSP
                     LILYLAAAGIGEIGIIDSDRVSLDNLHRQIVHDASSVGRSKTKSAAAAIERLNPNVAT
                     RLYSTRLVPENALGIIGGYDIVADGSDNFATRYLVNDACYFAKKPLVFAAVGPFDGHL
                     TTFRAFETDRAGNPRPSYRCLFPEPPPDGTVAPCSETGILGPVAGVIGSLAAVEVVKE
                     LLGIGERLVGRLLLYDALQARFSAVSYGWDPGNPLNGRAPRFRDLSHHRPILVGASE"
     gene            34125..35375
                     /locus_tag="VFY74_09225"
     CDS             34125..35375
                     /locus_tag="VFY74_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5931913.1"
                     /translation="MATEQIATSCAIAGGGPAGMMLGYLLARAGVDVVVLEKHADFLR
                     DFRGDTIHPSTLEVMHELGLLDAFLKLPHQKVSEIGGIVGDEMVTFADFSHLPTRCGF
                     IAFIPQWDFLDFLADQGKRYPTFKLMMQAEVDDLLWEGDRVAGLKAKLPDRTLEISAG
                     LVVGADGRHSKVRDLAGLEVEDLGAPMDVLWLRLSKRPGDGSQTLGRIQAGRLFVMLD
                     RGDYWQCAFAIPKGGFEALRAKGIEAFRNEIVALNPALADRVKEIASWDDVKLLTVRV
                     DRLKRWYRPGLLCIGDAAHAMSPVGGVGINLAVQDAVAAANILSEPLRMGRVAEEVLA
                     KVQRRREWPTKMTQAVQVFVQRRVISNVLSMTTQPRPPFAVTLFNRFPVLRRLPARLI
                     GMGFRPEHVETPVAPAQKEIPLSP"
     gene            complement(35542..>36276)
                     /locus_tag="VFY74_09230"
     CDS             complement(35542..>36276)
                     /locus_tag="VFY74_09230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX5931914.1"
                     /translation="LETARNRSILVTNTPGVLTEDTADMTMALILAVPRRLTEGAALL
                     KSEANGWHGWSPTWMLGHRIYGKRLGIVGMGRIGQAVAKRAKAFGLQIHYHNRRRVTP
                     DVEKALEATYWDSLDQMLARMDIISINCPHTPATYHLLSARRLALMKPSAYIVNTARG
                     EVIDENALARMIENNELAGAGLDVFEHEPAVNPKLLKSSKVVVLPHMGSATLEGRVDM
                     GEKVIINIKTFLDGHAPPDRVHPAMF"
BASE COUNT         6167 a        11860 c        11672 g         6577 t
ORIGIN      
        1 cgctctcttc actccgcttg caccgcccgc gcgcgcacta cgccgatcgg gcgccgaagc
       61 ttttttgagg accttctgtc tcatgggcca aaaacctctt gcatcgctcg aacgtgggtg
      121 taaatacccc gtcgtttagt cttgaacatc tgctggttgg ggaggacgcc atgtccagtg
      181 acgccctctt ccaattgggg atggacttgg atcatggttc aagcaccgcc caaacggaag
      241 aaaagctttc ccggtgcctc tctccaaacg gagagggacg caaggacttt ttctgtgagc
      301 gtgatggggt tcgttatgcc ggcacccatc tcatcatcga tctcgttcgg gcagagaggc
      361 tcgacgacct agaccacatc gagcatacgc tcagacgatg tgtagaggtt gccggcgcga
      421 cgctcctgca catccatctt caccatttca cgccgaacgg gggcgtatcg ggggtcgcgg
      481 ttcttgccga gtcgcacatc tccatccata gctggcccga atacggctat gccgcgctcg
      541 atgtcttcat gtgcggccag accgatccgc gggcggcgat cgacgtcctg aaagaggcct
      601 tctcgcctcg ccggatcgtc gtcaaggagc atttgcgcgg caaggaaata agtagatgga
      661 acgctgggtc gaagagacgc tccaccgagg cttccgtgtc aatctgaagg cggatcgcgt
      721 ccttttcgac agtgagaccg agcaccagcg tctgatcatt ttcgataatg ccgactttgg
      781 ccgggtgatg atgctcgaca gcatcgtcca ggtcacgacc aaggacgagt tcatctatca
      841 cgagatgatg gcgcacatgc ctttgttcgc ccacgggcgg gcgaagaggg cgctgatcgt
      901 cggcggcggc gatggcggcg tcttgcgcga ggcgctccgg catcccgcgc tcgaacaggt
      961 cacgctgtgc gagatcgacc gtggggtcat cgatctctgc cgccagtatt ttccggagat
     1021 ttcggccgga gcgtatgacg acccgcgcgc ccggatcgtc atcgccgacg gcacgaaatt
     1081 cgtggccgag acggccgacc gtttcgacgt gatcatggtc gattccaccg atcctgtcgg
     1141 gcccggcgcg gtgctgttca cgcgcgcgtt ctatgccggc tgccggcgct gcctgaagcc
     1201 gggcggccta ctcgtcaccc agaacgggct cccgttcctt caggcgggcg agctggagca
     1261 gagcgtcggt tatttccgcg agctgttcgc cgacgcgttc gcctatctcg ccacgacgcc
     1321 gacctatttc ggcggcccga tgtcctatgg ctgggcgacc gacgacaaga cgctgcgcga
     1381 gcataagcgc aagaagatcg cacgccgcca cgagaaggcc ggtgccttcg cgacgcgcta
     1441 ctggacccct gaagtccatg tcgcttcctt cgcccttcct tcttacgtgc aggaattggt
     1501 cgccgcctaa cctccctccc cgcgagggga gggtggcttg cgccgtttcg gcgaagccgg
     1561 gtggggtggg ggtggttcga gcgcaaggag aatcatctct agaacgccgt caatctctcg
     1621 atcgatctgg ttattccaga agcggagcac ctggtacccg ctcttctgca agtgatggtc
     1681 gcgcgcaacg tcacggcgtt ggtcgccgtc acaggcatgc tgtccgccat cgacttcgat
     1741 gatcagcctt gatcgacgac actcgaaatc gacgacatac ggcgtgatgg gagactgtct
     1801 ccgaaagtga tgtccgagga ctctcaagga tcggagccgc acccaaagcc tcacctcctg
     1861 gggcgtcatt cactttcgaa gtgatcgtgc agtttcgttg gccatgtcgc cccacccggc
     1921 tgccatgtct tgcgccatgg cagccaccct cccctcgcgg ggagggaaaa ctaaagccgc
     1981 tccatgcgcg gcaggtcacc caccagcggc cggcaatagg cgtggaaggc ggcgctcacg
     2041 tcgtttgcgc cggagatgaa ctccgccggc acatgccgcg tgcgccgcgc aacggcggcg
     2101 aggtctgcgc gggcgcaggc gctgcgatag gcgtcgggcg cgttcgaggt gcggacaata
     2161 attacggagg cggaagcatc cccccgggcc gcaagctccg cggcgtagcg gccggcggcg
     2221 cgcgcctcca acgtgtcgac cggcgagggc tcgggccagc agcgctggat atagccgaac
     2281 gtatcggcac gcacgcgggg agccttgccg cccttcggcg tgagcgctgt cttgacgaga
     2341 tcggcaagcc cgtcgccgag cgcacctgag cctgagagct gcacattgcc gtgggcgtcc
     2401 acctcgcccg gcccccgcat cagcttgggc ccgatctcgg cgccttgggc gtcgcgaatg
     2461 ccttcgctaa ccgcgatctg acagcgcccg agcttggcat agacggcctc gacatcggcg
     2521 agaaaagcat cggtcgagaa cgggacctcg ggcagataga tgagatgcgg cgcgggcctg
     2581 tgctcgggga gcgcgccatc gggcgccgca ctgcgggcga gcgccgccgc cgcggtgagg
     2641 aaccctgcgt tccgccccat gatgacgttg atcttgatgc cgccgaggct tgcattgtcg
     2701 agcgcatccc ccatcagcgc gagcgccacg tagcgcgcgg ccgaagggaa gcccggcgtg
     2761 tggtcgttct ccaggagatc gttatcgatc gtcttcggca catggaagca gcggagctcg
     2821 taacccgcgg ccaacgcctt ctcgctcacg atgcggcagg tatcggcgga gtcgttgccg
     2881 ccgatatagt agaaatagcg gatgtcgttc ttgcggcagc ttgcgaggat cttctcgcaa
     2941 taggcctcgt cgggcttgtc gcgggtcgag ccgagggctg cggagggcgt tcgcgcgatc
     3001 gcctcgagct tggccttgtc gagggcggtc agatcgagga gcccgtcggc ggaaggcttg
     3061 atgagcccgc gcacgccgtg ccgcatgccg aagatgcggc cgaccttgcc cgacgcggcc
     3121 agccccgcgc gcaggccttc gatcacgcct gcgagcgact gattgacgac ggcggtcggc
     3181 cctccggact ggccgatcgc cgcattgccg gaaacaagat cgggcacgtt ctacctctct
     3241 atcgcaacgg cgggcaccat gccccggacg cttgcgccct ggcaagattt ccctctcccc
     3301 ttgtgggaga gggcttcccg cccggcgcgc gaagcgcgac gcatggcggg aagggtgagg
     3361 ggtggattct tttgacgcgg gctatgcccg cttacccctc accgcgccgc gagcggcgcg
     3421 tgctctccct caaggggaga gcgggccaca atgctccagc tttggccgtt atcatgtgcg
     3481 atcgtgtcgc gcgccatcag caggagtgtc atcatgtcat cgcgggatgt ctggccggaa
     3541 ctgccctacg aagcgtggaa ggaaacttgc gcgaccttgc agctatggac tcagatcgca
     3601 ggcaagatcc gcctcaagca gacgccatgg ctcaaccact catggcacgt ggtgctctat
     3661 gtgtcgtcgc ggggcctgac cacctccccg attccgttcg gggatcgcac cttccagctc
     3721 gatttcgatt tcctcgacca catgctgtgg gtctcgacca gcgatggcgg aaggcgtcgc
     3781 gtcggcctct acccgcggag cgtggccgat ttctatgcag agctcatgcg ctccctcacc
     3841 gaccttggca tcaacatccg tattaatgag ctgccgaacg agatccccga cgcgatccgt
     3901 ttcagcgagg atcatgtgca tgcgtcctac gatggcgagt tcgcgcagcg cttctggcgc
     3961 attctcgccc agtcgacgca agtgatgagc agattccgca catcgttcat ggggaagtgc
     4021 agtccggtcc acttcttctg gggaagtttc gatctcgccg tgacgcgctt ctcggggcgg
     4081 ccggcatctc cgttgccctc cggcagtggc agtgtgccga acatgcccgc ttccgtggtg
     4141 ctcgacgcct attcgcacga gtgcagcagc gcagggttct ggccgggcgg tcaaggcgtc
     4201 gactatgccg ccttctactc gtacgccgcg ccgtcgccgc cgggctttga caaagcaacg
     4261 gtgcgtccgg ccgaagcctt ctggaacggg gacttgaacc aatacctgct tccatacgaa
     4321 gcggtccgca ccgcgagcga tccggaagag acgctgatgg cgttcttgac cagtacctat
     4381 gaagcagcgg ccaccgccgg taactgggac cgcgccgcgc ttgaatctcc gctcggcgtg
     4441 ccgggcgtcc cccgcaaggt ctgatgaggt ggactcatgc ggcgctcttc ccctctcccc
     4501 ttgtgggaga gggcttcccg accggcgcgc gaagcgcgac gaatggcggg aagggtgagg
     4561 ggtgaagcct tttgacgcgg cgccatgacc cctcaccgcg caccaagtgg tgcgcgtgct
     4621 ctccccaggg ggagagcggg cagtttattg caaggtcagc ccgtcatcct tcgaggctcg
     4681 ctgcgctcgc acctcaggat gacggaagga gaacgaggat cactcgagac gcaggagatg
     4741 caggctgaag aggatgtccg tgtcggtcca cgctttgaca gggcgccagc cgcccgcgct
     4801 cgccagcgcc tggaactcgt ccaccgtgta tttgtgcgag ttctcggtgt ggatgcgctc
     4861 gcctttggcg aagccgaagc cgcggcccaa gacgcgcacg gtctgatcgg cgaggctctt
     4921 gagatacatc tccatgcgtc ccgccttggc gttccacacc gcgtcatggg cgaagcggga
     4981 cagatcgaat gtgcccttaa gctcgcgatt gatgcgcgca agaaggttga gattgaaagc
     5041 ggcggtgacg ccgcttgcgt cgttataggc gggcacgagg atggaagccg gcttcttgag
     5101 atcgaggccg acgagaaaga cgcttcccct gcccaagagc cgcgccgcgt tcgcaagaaa
     5161 cgctttcgcc tcgtcatggc tgagattgcc gatggtcgag ccggagaaga acccgagccg
     5221 cttcttgctc cgcgctgcct gaggcagagc ccgcgtcgag gtgaagtcgg cgatgagcgg
     5281 caagatggtg aggcccggat gttgccctga cagccattcc gccgcctcct ccaggctttc
     5341 cgcggcgatg tcgatcggga tataggccgg aacccctctc agcgcgctca acaggaggct
     5401 cgtcttgcgg ctcgaccccg aaccgaactc gactagaacg cgcccctcgc cggcgagcgc
     5461 ggcaatctcc gatccgtaag cttcgagcag cgccgtctcg gtgcgcgtcg gatagtactc
     5521 ttcgagcttg gtgatctcct cgaacaattc cgagccgcgc gcatcgtaga agaaacggca
     5581 ggggatgctg cggggcttcc gcgacaaacc agcgagcacg gcggcggcaa agtcgtcctc
     5641 ggccgcttcc tccggcctcg cgccggagcg ggcggccgat cccgcggaag catgaagcga
     5701 gatgtcgcta cccatccccg ccctccgccc cttacccgtc ctgggccagg cgcaggccgg
     5761 tgaactgcca gcgctgatga ggatagaaga aattgcgata ggtgcggcgg atgtggccct
     5821 cgggcgtgac acaggagccc ccccggagca cgaactgatt gcacatgaac ttgccgttat
     5881 attcgccgac cgcgcccggt gcggccttga agcccgggta gggcaggtag gcgctgcccg
     5941 tccattccca tacatcgccg atcatctgct tcagcccttc gccggttgag gcggccatcg
     6001 gctggagaga accgcttcga agcgtgcggc cttcgagcgg cgcgcttgcc gcggccacct
     6061 cccactcgaa ttccgtgggg agccggtagc ccgcccagcg cgcgaaggca tcggcctcgt
     6121 aatagccgat atgcgtgacc ggtgccgccg gatcgatcgg gcggaagccg ttgaggctca
     6181 tttgcgccgc tccgccttcg tcgtcctccc aatagagggg cgcacgccag tcttgaccgc
     6241 gcacgacgtt ccagccatcg gcaagccaca gcgtcggcgt tgcgtaaccg gcatcggcta
     6301 tgaactcgct ccattcggaa ttggtgacgc agcgatcggc gagcttgaaa ggcctgatca
     6361 gcgtttcgtg acgcggtccc tcgttgtcat aggcaaagcc ttcgccttcg tgcccgacct
     6421 cgacgatgcc gccatcgaac gaaagccagc tgatcggctc cgtctcgccg gccggaacgc
     6481 cgggatcgac ctcgcggtaa gcgggcttga gcggctcggc agcgaacagg ctcaagatgt
     6541 cggtcagcag aagctcctga tgctgctgct cgtgattgat gccaagctcg atgagcgctg
     6601 ccgcatccgg cgcgagcgga ccgtcgaaca ggcggtcgag cgccgcgtcg acggaggcgc
     6661 gataggcgcg cacctcttcc gccgcgggcc gcgtcaggag cccgcgcttc gggcgcggat
     6721 gcctcgggcc cacgctctcg tagtaggagt tgaagcaata ttcgaagcgc tcgtcgaaga
     6781 cgcgatagcc tgcgaggaag cgcttcaaca ggaacgcctc gaagaaccat gtcgtgtgag
     6841 cgagatgcca tttggccggg ctcgcatcgt ccatcgcctg gacgacctgg tcctcgtcgc
     6901 tcaaggggct ggtaagctct gacgacaacg cacgcgtcgc gaagagccgg tcgcgcaaga
     6961 cgggcgattc ggtcttgggc ttttcggctg tgctaaggac tgcgctatgg aggttcatca
     7021 tcagctccct cggattaggg caagtcgccg ggaaaagccc ggatccgccg agccgacgcg
     7081 ccaccaccga aacgcgagat ttaccagctt gttccggggc ggggaatggt taagcatgca
     7141 caactctgtg agacaaattg attttgccgg agtcctcgca tgcaggacac ccgcgccagg
     7201 gacgacgcga tcataccgtt cgacgggcga caatccccgg tcgccgcggc gttgcagcgt
     7261 ggcgtctgcc gcgcacttcg cgcgcgcggg cactcggtcg ttaccgaact ctgtctcgcc
     7321 aacggaaggc gcgccgacgt ggtagccctg tcgggcgcgg gcgacatcac gatcgtcgag
     7381 atcaagtcct gccttatcga ctatcggagc gacggcaagt ggcaggacta cggtccttac
     7441 tgcgaccggc tctacttcgc cgtcgcggcc gacttcccgc gcgaaatcat cccgccgcct
     7501 gttggcctca ttctcgccga tcgttacggc gcggagcttg tgcgcgagcc ggtcgaagaa
     7561 cgactctcgg ccgcgcggcg caaggcgatg atgctcgcct tcgcccgtgc cgcggcactg
     7621 cggttgcagc tccacctcga tccctcttgc ggttttgagg cttgagccgg ctcgtcatgc
     7681 gaggtgcccg cgaagcggcc tcgaaggacg cagtgtgcag tcgtcatcct tcgaggctcg
     7741 ctccgcttac acctcaggat gacgccttat atcggacccc gctctcccct taagggagag
     7801 cacgcgccgc ttgcggcgcg gtgaggggta agcgggcgca agcccgcgtc aaaagagatt
     7861 cacccctcac ccttcccgcc actcgtcgcg cttcgcgcgc cgggcaggaa gccctctccc
     7921 acaaggggag agggggatga cccccgttac ttcggggcgc gcttggcgag gatgcgctgc
     7981 aatgtgcggc gatgcatgtt aaggcgccgc gccgtctccg agacgttgcg gtcgcagagc
     8041 tcgtagaccc gctggatgtg ctcccagcgc acgcggtccg ccgacatggg gttttccggc
     8101 ggcgccgcct tgaccgtcga ggccgaagcg accagcgcgt tgtagacatc gtcggcgtct
     8161 gcgggtttgg cgagataatc gaccgcgccg agcttcaccg cggtgacggc ggtggcgata
     8221 ttcccgtagc ccgtcagcac cacggcgcgc gcttcggggc ggacctcctt gagtgcggcg
     8281 atgacgtcga tgccgttccc gtcttccaga cgcatatcca cgacggcgaa ggcgggtgga
     8341 cggcgtttga gcgacgaaag cccgtcctcg accgaagccg ccgcctcgac ctcgaagccg
     8401 cgcgcttcca tggcgcgcgc gagccgggcg aggaaggcgc gatcgtcgtc gacgatcagg
     8461 agcgtgttgt ccgcgagctt gcccgaatgg gcttcggtgg aaggcaaggc ctgatcagct
     8521 tccatttccc gttattcctt aaagcaattg tccaagacgc aattccccga acgtgcggga
     8581 gattttctgc ctatggccct caacagggcc tagacctagg cattattccc cccaacggca
     8641 agcctctcct cgctcccgat cgcgggcgtg acgcgcccgc cgcagtgcgc ccccgtctct
     8701 tggccctaag cgaaagcggc cggctcggcc cgtgcgcccg cctccccggg ggcctgctcg
     8761 aacctcgcgc gcggccacat caccgtcacg accgcaccgc cccggttggc aagttcgagc
     8821 cgggcgcccg aacgctctag caacgtcttg gcgataaaga agcccaaacc catcccgaca
     8881 tgctcgtcgg gcgtgtcggg tccatcggtt cgggtcgccc gcgtcgtgac gaaaggctcg
     8941 ccaagctgct cgagcacgtt cggcggaaaa cctgttccgt cgtcggcgat aacgatcctc
     9001 acctcgtcgc tcgaccagcg cgcttcaaca ttgaccttgc tcgcggcgaa atcgacggcg
     9061 ttctcgacga tattgccgag accgtaaaga acgccgggat tgcgctcgct cacgggctcc
     9121 gcaagcgtca cgccgtggga gccgccgtcc tgcgccggcc cgatccgaac ctcgatcggc
     9181 acagccatca cgcggtgcgg cgccaccact tcctcgatga ggtgactcaa ggatagccgc
     9241 gatacgatca gatcgcgctg ctcgccgccg cccccgatct cggcgaggat gtcgcggcag
     9301 cgctcggcct gccctctgag caacgaaaga tcgtcataat gcgggtcgtc gggcccggcg
     9361 ccccgtatca gctccttcgt caccaccgcg atggtcgcga gcggcgtgcc gagaccatgc
     9421 gccgccgcgg cggcaagacc gtcgagcgcc gaaagcttct gctcgcgggc aagcaccatc
     9481 tcggtcgcgg cgagcgccct ggccatgcgc cgcgcctcct cggccgtgcg ccaggagtag
     9541 aaggcgatga acaggatgcc cgagacgacc gccgcccacg cgcccatcac ataggggaag
     9601 ggaagcagga ggttcgtatg gttgaaccag gggaggggat gatgaaaggt ggtaagaacc
     9661 gaggccgcca ggatggcaag cgccccgagg gccgccgtga tcccgagagg caaggtcgag
     9721 gccgacacag tgacgggtgc aatgaggagg aaggcaaagg ggttttgcag accgccggtc
     9781 aggtagagca acacggcaag ctgaaggata tcgtaaccga gcatcgcgaa ggcgaaactg
     9841 ctcttgagcc gcttgctcgc cggatagcgc aagcgtaagg ccacgttgag cgaggcggac
     9901 aaggcgatga cggcaaggca gcccgcgatc ggcaaatcga tcccgagccc ccaataagcg
     9961 ccgatcacgg tgatgctctg gccgagcaca gcgagccagc gcaagcggac gatggtttgc
    10021 agcctgagcc tggtctcgcc ctgcctgagc caggcgggca cagtcgcgcg gcggatggtt
    10081 tgactattgt cgacggcgtt catagctcaa gtctaccatg gcggagcagt cttaattccg
    10141 acaaaccgaa cgcgcagcgc aaagctcatg aacgataggg ttagtaaagt cggccgggac
    10201 atcggagcgc ccgcggcagc cggaaaaggg cctgacggga aggacgtacc cgcgaaggtg
    10261 cgcgacctca ccaagatcta cggcgccgtc accgccgtcg accgcatcag cttcacgctc
    10321 gcggaaggaa cgacggttgc gctgcttggc ggcaacggcg ccggcaagac cacgacgatc
    10381 gccatgctgc tcggtctggt cgtgccgagt gcgggcgagg tcagggtgtt cggcgaagat
    10441 gtgagccgca atcgcggcgc ggtcgcccat cgcatgaatt tccagagtcc ctatgtcgat
    10501 cttccggcgc ggctctcggt gcagcaaaac ctcaccgtct atgccggact ctacggtatc
    10561 gccaacgcgg tcgagcgcgt cgcctatatt gccgaggagt tgcagatcgg gccgctgctc
    10621 gatcggccga ccggaaagct ctcggccgga cagaagacgc gggtggggct ggcaaaggct
    10681 ctcctcaacg cgcctgatct gctcctgctc gacgagccca ccgcctcgct cgatcccgac
    10741 acggcggact gggtcaggcg caagctcaag gactatgccg acacccgcgg cgccaccctt
    10801 ctgcttgcct cgcacaacat ggccgaggtc gagcggctcg ccgatcgcgt gatcctgctc
    10861 gatcagggac gcatcataga ggacgagacg ccggccgatc tgattgcgac ctatggacgg
    10921 gaaacgctgg aggaagtgtt tctcgacgtg gtgcgcggac gcgccaaaca ggatcccgat
    10981 gctgtcgacc gcggtgccgc accggaacgg aaggcatcgt gaccacggcc ccgaaaatag
    11041 cgcaaggcgc gacgcccatc atccttccgc cgacgggcgc gagagcatcg ctccgccgca
    11101 tcggcgccct gctggagcgc tacatctatc ttctgcggag ctcgggcatc cgtctgctcg
    11161 agctgatcta ctggcccttc cttcagatgc tcacctgggg cttcctgcag acatacttgg
    11221 ccgagaccca aagccccgtg gcgcaagccg cgggcgtcct gatcggctcc gtcctgctct
    11281 gggacatttt gttccggtcg aagatcggct tctccacgac cttcatcgag gagatgtggg
    11341 cgcgcaatct cggcaatctc ctgatcagtc cgttgcgtgc ttccgaactc gtcgcggctt
    11401 tgagcctttg gagcgtgatc cgcctttcgg tgagcatgat cccggttgct ttcgcggcct
    11461 atctcatctt cgggttcaat ctcctcgaac tcgggtttgc gctcgctgcc ttcttcgccg
    11521 tcctggtcct caccagctgg tcgctcggac tcatctcggc gggggtcatc ttgcgttacg
    11581 ggctcggcgc cgaggagctc gcctggtcgc tcgcctttct gctgctgccg atctgctgcg
    11641 tttactatcc cgtatcggtg ctcccggaat ggctccagcc cgttgcgctc gcgctgcctc
    11701 cgacccacgt gttcgagggc atgcgggcga tccttctcga gcatcgcttc gatgcgaacg
    11761 atctgtggtg ggcgctcggg ttgaacgccg tctatctcat cgtcggctat ctggtcttta
    11821 tccgctttct cgccagcgcg cgcgtcaacg gctcgctgct gcaattgggt gaatagatgc
    11881 gcacgcgcct catcgtcgtg atcgttgcgg gcttcctcat cggcgcgatt gcaggggcgg
    11941 ccgtgctggc gctcaccggc aacggctcgg gccctcaggt cgcgacctcc ggcaaggcgc
    12001 tgatcggcgg acccttcgcc ctgattgacc agaacgggaa gacggtcacc gatcagaatt
    12061 tccgcggccg tttcatgctc gtcttcttcg gcttcaccca ttgtcccgac atatgccccg
    12121 ccgagctcca ggtcatttcg caaagcctcg aggcgctcgg gccccaagcc gaggaagtgg
    12181 tccccatctt catcacgctc gaccccgagc gcgacacgca ggcggtcatg gacgcatacg
    12241 tcaagaattt cgggtcgcgc ttcgtcgggc tgaccggctc gcccgaggcc gtcgccacgg
    12301 cggcgaaggg gtatcgtatc gcctactcga agttccagga ggacccggcg tcgagcgatt
    12361 acagcgtcga ccactcagcg ctcgtctatc tgatgggcag gaacggcgaa tacgtcacgc
    12421 atttcgccta tggcacgccg gccgacaaaa tgacggaaac gcttcgccgc tacctttagc
    12481 cgcgcggcgt gtttgccccc gcgacatcct gtccttgcac tgcaacatag aatcaccatt
    12541 tgattctgcg atgcagcatt tactgtatcc tcggacggga gggacaattc ttgttgtacc
    12601 atttctatga gctcaaccgc gcggccttga gcccggcgcg ggccgcggcg gacgcctacc
    12661 ggctgctgtt ccgcaacccg ctcaatccgg cctattacac cccccttggc aggagcgctg
    12721 cagctgcgct cgaactcttc gagcgcacca cgcgtcgcta ccgcaagcct tcgtggggca
    12781 tcgacaagac caaggtcagg ggccgggagg tcgccgtcag cgagcgcgtc gtcatttcca
    12841 agcccttctg caatctcgtt catttcgagc gagaggggtc gcgctggcgc cttcgcaatg
    12901 accccaagct cctggttgtg gcgccgatgg cgggccattt cgccacgctt ctccgcggca
    12961 cggttcgcga tctgcttccc tattacgatg tctatgtcac cgactggcag gacgcgcgct
    13021 tcgtgccgcg cgcagcaggc ccgttcgacc tcgatgtcta tatcgactat atcattgaat
    13081 ttcttcgcta tttccgcggt gacgtgcatg tgatggcggt atgccagccg gccgtgccgg
    13141 tctttgccgc ggtcgccctg ctcgaaggga tgggggatcg ttcaagcccg cgctcgatga
    13201 tcctcatggc cggcccgatc gatgcgcgcg agaatccgac cgccgtcaat cgcttcgccg
    13261 aggcccgcac gatcgactgg tttcgccgca acgtcatcgc ccaggtcccg tggccttatc
    13321 cgggcgtgat gcgcatggtc tatccaggat tccttcagct cagcggtttc atggggatga
    13381 atttcggccg ccatgtctcg gcgcatcgtg atctttttca taacctgatc aagggagatg
    13441 gcgactccgc cgacaagcat cgcgagttct acgacgaatt tcttgccgtt atggaccttg
    13501 cagccgaata ctacctgcag acgatcgaga ccgtctttct cgagcacacg ctcgccaagg
    13561 ggcagatgac ccaccgcaac cgcctcgtcg aaccaagcgc aatcaggaaa gttgccctga
    13621 tgacggtgga gggcgagaag gacgacatca ccgggatggg tcagaccttt gctgcccaaa
    13681 ctttgtgcac caaccttccg aacaccatgc gggcacacca tcttcagtcc ggcgtcggcc
    13741 attacggcgt gttcaacggg tcccgctttc gtgctgaaat tgtcccgaaa atccgggatt
    13801 ttatcgcgac gcaccagcgc cggggaggcc tcttggcgcg ggccttcggc agggctgcct
    13861 aaagctcagg cgagcgttcc cgggacacgc tgtgctactg tacttgccgc cgaatcgggg
    13921 gaccttcggc ggtagggagc gtatcgatgt taaagcggct caatctcgcg cgcggcgaga
    13981 ccatagaggt caaaatccgc gggctcgaca tccccatggt tctccggcgg agcgcgcgag
    14041 ccagacgatt cagcctgcaa gtgaacgaag cgcgccgcgg tgcggtgctc accatgccgc
    14101 tctattcggg cttcgccgat gccgacgaat ttctctcgcg tcatctcgat tggctgaagg
    14161 agcgtgtcgc cggactgtcg gcacccgtgc ctttcaccca cggcgccatc gttcctctgc
    14221 gcggacgggc gcatgtgctt cgcttcgcgg gcctcgtgcg ccgccgcggt gtcgtgtgga
    14281 tagaggccgc cgaagacgcg aaggccgcgc cggcctggcc cgaaggggcg cgcgtgggcg
    14341 tgaggcgcct gccgcgtctt tatgtggcgg gtgcggacga gcatgcgccg cgccggctgc
    14401 tcgactggct gaagcgtgaa gcccatctcg atctgaaagc gcgcgtcgag caccatgcgc
    14461 gccgcctcaa cctggccccg aagcggctct tcgtgcgcga ccagaccacg cgctggggat
    14521 cgtgctcgac ctcgggcgcg ctgtccttct cctggcggct cgtgctcgcg ccgcctttcg
    14581 tgctcgacta tctcgcggcc cacgaggtcg cccatctcgc ccacatgaac catgggcccc
    14641 gcttctgggc tctcgtcgag cgcaccatgc cccgccatca agaggcgcgg agctggctta
    14701 ggaaacatgg cgcgagcctg catcgctgcg gcgccgaggg ggtgaagccc tcgtcgcgca
    14761 cctcgccgaa gcgcagcatc gaatagcggg aggtgaccat caccattacc gcctttcaac
    14821 ggtcacccga tggcggccag ggactagcgc gtgatacccg cgttcgctgg gcacttgaag
    14881 aagtgggcca accctacgag gttcgccttg tttcgttccg tgcgatgaag gaacccgctc
    14941 atctggcgct tcatcccttc ggccagattc cgacctacga ggaaggcgat ctcgccctgt
    15001 tcgagacagg gtcgatcgtc ttccatatcg ccgagcgcca tgcgggcctg ctgccggacg
    15061 ataccgatgc gcgggcgcgc gcgatcacat ggatgtttgc cgcgctcaac acggtggagc
    15121 cgccgatcct tgaactcgca aacgccaggc ttctggaggt cgataagccc tggagtaagg
    15181 agcgcctgcc tctggtcgag gaccgcgtcc gcgaccggtt gaatcaactt tccgctcgcc
    15241 tgggcagtgc cgctggctcg atggtgggtt tagcgcaggc gacctgatga tggtgtcggt
    15301 gctgctcagg ctaaaatcat cgggcattct ggacgaatat ccgaacctgg ccgcctatct
    15361 cgcccgtggc gaagcgcggc ccgcctacaa gcgggctttc gaggctcaat tggcggttaa
    15421 cacccgcaag ccaccggccg gctgattgag ctccgttctg cgctcgaagc cgtcatccgg
    15481 gtttttcgct cagtttatcg gtcccgacct taccgaccgc cgaagaatcc gaacacccgg
    15541 cgaaggaacg gcgtatcgcc gccgcttgag ctttgccacg gcagcgccgc catcgcttcg
    15601 ggaagccgcg gaccgttgct gccgggcaga ggcagcggcg gcttttcctg gtgcgcgtaa
    15661 agcatgacat cgtgccagag cgtggcggga agcgttccgc ccgtcacctt cttcatccgc
    15721 ctgccgttgt cgttgccgat ccacaccccg ccgacaaagt gggcggtgta accgacgaac
    15781 caggcatcgc ggaaactctg tgttgttccg gtcttgcccg ccgcctcatg gccttcgatg
    15841 gcggccttct gccccgtgcc gaacctgatt gtcgcgttca gcatgtcgtt catcgcgccg
    15901 acatcgttga gcgccacgac ctggccgatg gtcgagcgcc ggcgctgata gagcaccttg
    15961 ccgtctccgt cacgcacgcg tgtgatgatg tgcggcagga cgccatgacc gccattggcg
    16021 aagggcgcat aggcgccggc gagctcgagc agcgtgacct cggccgtgcc gagcccgatc
    16081 gagggccggt catgcagctc ggaatggatg ccgaggcgct ctgccgttcg cgccacctgc
    16141 gaaggtccga ctttggcggt gattcttacg gccacggtgt tgatcgagtg ggcgagactg
    16201 tcgcgcaggc tgatctcgcc gcgatacttg ccggtatagt tgcgcggcga ccagcctgcg
    16261 accgtgatcg gcccgtcatg ggcgagcgag tccggcgaat agccggcctc gagagcggca
    16321 agatagacga acggcttgaa ggcggagccg ggctggcggc gcgccttgag cgcccggtcg
    16381 aacgggcttg tcgaataggc gcggcctccc accagcgcct ttacctcgcc ggaaggatcg
    16441 agcaccacca ccgccccttc actcgccgca accgctgtgc cctcgctgtc gaggatgcgc
    16501 cttagagcct gctgcgaggc gcgctggagg cccgcatcga tcgtggtctc gacgaagagg
    16561 tcgccgtgct gctcgcccat gagttcgggg aggagctcgg ccacccagtc gaccgcgtag
    16621 gcataacccg tgtcgtcgtc cttggccgag agcttgagcg gctgctcgtt ggcgttccgt
    16681 gcctgctctt ccgtgaggta gcccgcctcg accatgttct cgagcacctc atcgacgcga
    16741 atgccggcgc gcttggcgct ccgtgtcggc gcgtagcgtg agggcgcctt gagcagtccg
    16801 gcaagaagcg ccgcctgggg cagcgtgagg aagcgcgaag atttgccgaa atagcggcgc
    16861 gaggcggcct caacgccata ggtgccgccg ccgaaataga cgcggttgag gtagagttcg
    16921 aggatctcgt ccttggagaa gcgctgctcg agccagagcg catagacggc ttcttcgagt
    16981 ttccgcgaga gggtgcgctt cggcgtgagg aacaggttct tggcaagctg ctgggtgatg
    17041 gtcgagccgc cctcgaccac ggcgccggag gaggcgttgc gaagcgaggc gcggatcagc
    17101 ccaagcggat cgacgccgaa atgggcgtag aagcggcgat cctcggtggc aagcacggcc
    17161 tgcaccaggt aaggcggaag cgcatcgagc ctcatatggc cgcggcgcaa accgcgctcg
    17221 gcgatcaccg cgccgtcttg ggcgagcacg gtgacattgg gcggccggtc gtcgagcgcg
    17281 gcgcttctgg gatccggcat ctgcgcgatc atatagatcg ccatcacgcc gatgacggcc
    17341 gcgccgcaca gggatgcgac cgccgcatag cggagtgcgc gggcgagcca gggatggcgg
    17401 cgcgcccagc gcttcagccg cgagggccgg cgccgcgcgg acccggggcg cgggttcgcg
    17461 gacccacgcc gcgcgccgga actgtgctga ggcccgcgac tgactttcgc gccgggcctt
    17521 gccgtgtgcc gcgcgccctg catgtggttc caccccgtgg agacaagcaa acgcagagcg
    17581 ccgaccggcc ttggagcctc tcggcactcc atggggcgaa gttaggcgtt ccgccttgat
    17641 acgacgttaa aggattgcgc caccatgcct gttgggccgg cgtgaaatcg tcagccgaca
    17701 tccggtctcg caaagcaaac tccgtcatgg ccgggcttgg cccggccatc cacgatttgg
    17761 caaactcacc gcatgcgagg ggggtgggtt tacattttaa cgaaccggcg aaacggcatt
    17821 ctctacacgg gcgttgcgcg cgatctcgcc cgccgcatct ggcagcatcg ggaaggcctc
    17881 ggcggcgtct tcacgcgacg ctacggcctt aagcgccttg tttggtgcga gtaccacgac
    17941 accatcctcg gtgccattca gcgcgaaaag aacctgaagc actggccgcg agcgtggaaa
    18001 gtgcggctga tccacgcggc gaatcccgat tgggacgacc tctacgagag gctcaatcaa
    18061 tgactcggac ttcaatcgtg gatggccggg ccaagcccgg ccatgaccgg gaacttgctt
    18121 ttaggagttc ggcaggcaaa cctcgaactt cacctgccac tcctcgtcat gcccggcttg
    18181 acccgggcat ccatgccgat acgtctcaac gcgttgaagc gccattggaa tggattgccg
    18241 ggtccagccc ggcaatgact ggcggatgtg gtttcgtgtg aggtgagtca acggacgacg
    18301 accgagaggc cagatcaggc tacacgtcga ggttggcgac ctggagcgcg ttggcctgga
    18361 tgaactcgcg ccgcggctcg accacgtcgc ccatcagccg cgagaagatg tcgtcggcgt
    18421 cctcgccctc cttgatcttc acctggagga tggtgcgggt gtcgaggtcg agcgtggtgt
    18481 cccacagctg atcggggttc atttcgccaa gccctttgta gcgctggagc gtgagcccct
    18541 tgcgccccgc cgtcatcacc gcatcgagaa gctcgctcgg cccgtgcacc gcgaggctcg
    18601 tgtccttgcg cttgagcgtc gcgggccgcg catagacctc ctgcagatgt tcggcgaagc
    18661 cgtcgagctt gagcgcgtcg gccgaagcga tgaacggccc gtcgatcaga tgagtctcca
    18721 tgacgccgcg caactcgcgc cagaagcgca agccgccctc cggcagggcc tcgccgcgcc
    18781 agccgcgctc ggtctcctcg gccaaggcgt cgagccgctt ggcgatatag gcggcggcct
    18841 gttgcgcctg cttgatgtcc ttcagcacct tcggattgag cacgcccgcg atcgccgcct
    18901 gctcgacgat gaagcgcgga tagcggctgt gcaggccttt gaggatcacc ccgatgcgcc
    18961 gcgccgtctc cacgatgtcg cgcagatcgg cactggcccg cgtcgcgctt ccggtgacga
    19021 gcaccgcgtc ctccagcccc tcgccgatga gataatcctc gagcgccttc tcgtccttta
    19081 agtagcgctc ggattggccg cgcttcacct tgtagagcgg gggctgcgcg atgaacaagt
    19141 gcccggcctc aaccaggctt tgcatctgcc ggtagaagaa ggtgagcagc agcgtgcgga
    19201 tatgggcgcc gtccacgtcg gcgtcggtca tgatgatgac cttgtggtag cggagcttgg
    19261 cgagatcgaa ctcgtcatgg ccgatgcccg tgccgagcgc agtgatgagc gtgccgatct
    19321 cggcgctcga cagcatcttg tcgaagcgcg cccgctcgac gttcaggatc ttgccgcgca
    19381 aggggagcac ggcctggaag gcgcggtcgc gcgcctgctt ggcgctgccg ccggccgaat
    19441 cgccctcgac caggaacagt tcggacttgg cgggatcgcg ctcctggcaa tcggcgagct
    19501 tgccgggaag cgaggcgagg tcgagcgcgc ccttgcgccg cgtgagttcg cgggccttgc
    19561 gcgcggcctc gcgcgcggcc gcggcctcga cgatcttctg cacgatgttg cgcgcctcgc
    19621 ccgggttctc ctcgaaccat cccgagagtg cggcggccat gacgctctca accacgggac
    19681 gcacctccga ggaaacgagc ttgtccttgg tctggctcga gaatttcggg tccggcacct
    19741 tgaccgagag cacgcaggtg agcccctcgc gggcgtcgtc gccggtcagc gccaccttct
    19801 ccttcttggc gatgcccgac tccgcgtcat aggtgttgac ggtgcgggtc agcgccgcgc
    19861 ggaagcccgc aagatgcgtg ccgccgtcgc gctgcgggat gttgttggtg aagcagagca
    19921 cgttctcgtg atagctgtcg ttccaccaca aggcagcttc caccaagatc ttctgctgct
    19981 ccttcgcaac gacgatcggc tcttggatca gcggatgctt ggtgcgatcg aggaaacgga
    20041 cgaaggcgcg caagcctccc tcgtagcgca tctcctcgac cttgggctcg accccgcgat
    20101 tgtccgacaa gacgatgcgc acgcccgaat tcaggaacgc aagctcgcgc aggcgatgct
    20161 caagcgtcga atagtcgaac tccaccattg tgaaggtctt ggtgctcggc acgaaagtga
    20221 tgcgcgttcc cctcttagcg ccggcatcgg cgacgggctt gagcggcgcc tccgccacgc
    20281 cgtcggcgaa gcgcatgaaa tgctccttgc cgtcgcgcca gatggtgagg tcgagccttg
    20341 aggacagcgc attgacgaca gagacgccga cgccatgcag cccgcccgag accttgtagg
    20401 cgttctggtc gaactttccg ccggcgtgga gctgcgtcat gatcacttca gccgccgaca
    20461 cgccttcgcc cttatggatc tcggtcggga tgccgcgccc attgtcctcg acggtgacgg
    20521 agccgtcggc attcaagatc acggcgatct catcgctgaa gccggcaagt gcctcgtcga
    20581 tcgcgttgtc tacgacctcg tagaccatgt ggtgcaagcc ggagccgtca tcggtgtcgc
    20641 cgatatacat gcccggccgc ttgcgcacgg catcgaggcc tttcagcacc ttgatcgatt
    20701 ccgcgccgta ctcggaggca gcggcttctt tgcgtttgga cgctggtgtc atggttccct
    20761 ggaaacggtt ctgggcgcct ttggcgggtg gccgccgagg cacgtgaatc aggccccgat
    20821 cataccccga ttcacagcaa atatttgagc gtcttgcgcc agcggcgcga acaagtcgcg
    20881 gtcggtcccg gttatccaag cctggcagcc gagggccaga atctggccga aaagggcctc
    20941 ccggcgggcc tcgtcgagat gggccgcgac ctcgtccaaa aggacgagcg gcgccacacc
    21001 ctcaatctcc ttgatcagct cggccttggc gaggatgagc ccgatcagaa gcgccttctg
    21061 ctcgcccgag gagcactcgg tagcgggcgc ctctttgggg ccatgagtaa caattaggtc
    21121 gctcaaatgc ggtccttcaa gggcgcgcgc ggccgcccga tcgcgctcgc ggccctcctc
    21181 gagagaggcg cgaaattcat cctcgacctc ggtcgcggcg tgcgtggcaa gagccgcttc
    21241 gagccgccct tcgagcgcta ccgacgccca ggggaaggga ccttcgccac gctccgcgcg
    21301 catgcgctcg atcagcgtga cgagccgccc cgtggcgtcg agccgcgccg cggcgatcgc
    21361 gacgcccgct tccgccatct gttcctcgag cccctcgaac agggcgcgcg atccctcccg
    21421 catctggaag agccggttcc tctgccgcat cgcccggtcg tagcggccaa gagggctccg
    21481 catcttgggg tcgaccgcaa ccacgagccg gtcgagaaag cggcgccgct cggaagccgg
    21541 acccgtgaac aggccgtcca tggcgggaat gagccagacc agattgacat attctgcaag
    21601 ggcgcccgcg ctcctctcct tgcccgcgat gcgcacggtc cgccccgtcg cgtcctcgtt
    21661 ctcgccagcg gcaagtcccg tgccgatctc gacctcgtcc tcgcccgaca cgacccttgc
    21721 cgcaacggcg aagccgccgg cgccctcttt ccgggcgagc tcgacatagg ggcgcccgcg
    21781 gagcccatgg cccggaccga gaagcgagac ggcctcgagg agattggtct tgccggcccc
    21841 gttttcgccg aacagcacga cggggcgcgc atcgaggtca agctccgccc gctcatagtt
    21901 gcggaaatcg gcgaccctca agctgcgaac gaagaaaagg gtcttggcag ggtcgccggc
    21961 gatcgcctct gccgccccct cacccttgcg atctgccctc atacccgcat cggcatgagc
    22021 acatagagcg cggaggcgtc cttgccgtcg cgcaccatgg tgggggaacc gggatcggcc
    22081 agctgaaact cggccgtgcc gctctcgagc tgcgccgaaa tgtcgagcaa atagcgggcg
    22141 ttgaagccga tctccagggg atcgaagcga tattcgacgg agagctcctc ggtggcgctg
    22201 ccgctgtccg gattgttgac cgaaagcgtg agcttgccct cgttgatcgt gagcttgacc
    22261 gcgcggccct tgtcgctcga caaggtcgag acgcgatcga cggcctgggc gaaagcggcg
    22321 ttgtcgacct ccatcgtctt gtcgttggat tgggggatga cccgctcgta atcggggaag
    22381 gtgccgtcga tcagtttgga ggtcagcacc acgcgcgccg tggacacacg gatcttcgag
    22441 ggcgacagct cgacgcgaac ctcgccctcg gcatcctcga cgagctttgc gagctcaagc
    22501 acggctttgc gcggcacgat cacgccaggc atgtccttgg cgcccggcgg aagcggcagc
    22561 tcgatctggg cgagcctgtg cccgtcggtg gcgaccgcgc gcaacaccgg cttgccgccg
    22621 ctttgcgccg cgtggagata gatgccgttc agatagtaac gggtctcctc ggtcgagatg
    22681 gcgaagcgcg tcttgtcgat cagacgcttc aactcggaag ccggcaggct gaagctgcgg
    22741 ccgagatcgt cgacactgag cgcggggaaa tcatcggcag gcagcgtttg cagcgagaat
    22801 tgcgcagggc cactgtgaat cgagagacgt ccctgctcgc cgtctcgtgc caactcgatc
    22861 tcggcgccct cgggaagctt gcgcacgatg tcatagaaca tatgcgccgg cacggtggtg
    22921 cccccttcgc ccttggcgcg caccggcacg gtctcgacca gctccatatc gaggtcggtg
    22981 gcggtgagct tgagctcatt ccggcccgcc gagagcagga cattggaaag gatgggaatg
    23041 gtgttacggc gctccaccac gctcgccaca tgggccagcg ccttggcaag tccgggtttg
    23101 tcgatggtga gccgcatagt gcccgatccc cgaaaaagtg acgatttccc gccggtaagc
    23161 tcagccctgg ccgaagcgct tctgctccag tccgaccctg acgaaggcgt ggatgaggtg
    23221 ccctaagggc ccgaacttga cgcgagagag gggaatttgc aagagcggcg gggggacgcc
    23281 ttggcttggg atccaagccc cccatccgcc ttcccctcat tcccgtagca gacgcttcag
    23341 aagctcgatc tcctcacgga gctgggtatc gtcgttcatc aactgctcga tcttgcggat
    23401 cgcgtgcaag acggtggtgt ggtcgcggtt gccaaaccgc cgcccgatct cgggcagcga
    23461 gcgcgaggtc agcagcttgg cgagatacat gccgatctgc cgcggccgca cgatcgagcg
    23521 gttacggcgt cccgacagaa gatcgccccg gttgacgccg taatgcttcg ataccgtgcg
    23581 caggatgtcg tcgatgcgca cgcgccgagg ctcggccccg cgcatcagat cgcgcacgat
    23641 ctgctctgcg gtttcgagcg tcacgggttg atctgtgagc tggaagctcg cgcgcaggcg
    23701 atgaaccgcg ccctcgagct cgcgcccgct ctcggtcagg cgttcggaaa ggaaggcgag
    23761 cacggtgtcg gagatggaaa ggcccttggt ttcggcgcaa gcctccgccg ctctcttttc
    23821 caggatcttg tgccgcagat cgtagtccat ggcgccgatc tcggcgacca gtcctccgcc
    23881 cagccgcgag cgcatccgca tgtcgagctt gtcgagctgg gtcggcgcgc ggtcggcggc
    23941 gaccacgacc tgacgcccgc cgtcgatcag cgagttcaag gtgtggcaga actcctgctg
    24001 gatggtcgga ccttgcagga actccatgtc atcgatcagg aggatgtcga tccggcgcag
    24061 cttctccttg aaggcgagcg cgtcacgcgc gcgcaaagcc tggacgaagc cggacatgaa
    24121 gcgctcggcg gtgagataga ggacctgcga ctccggcttg cgtttcttca cgtcccaggc
    24181 gatggcgtgc agcaggtggg ttttgccgac cccgacattg ccgtgcacga acaggggatt
    24241 gaagagaggc tgaggggccg agaacgcgcc ggccacctcc tgcgccgcgg cgtaagcgag
    24301 gcggttggag gttccgacca tgaaacttac gaaactgagc ctcggatcga gcggcgagcc
    24361 ctcgaagccg ccatagccgg tcctcaaggg gagcggaagc ggtgagagcg cctcgggcac
    24421 ggggggctcg ggactgcgcc gaaggcgcgg ctgctccttg ggtgcctcga cgccgaaatg
    24481 cggctgcctc accctgaatt cgacgcgctc gacttccggc cactcggcgc gcgagagcct
    24541 caagacgaag tcgtaatagt gcgagcggag ccagttgcgg aggaagcgag tcgccacggt
    24601 gagatggacg acctcgccgt ccacctgctc gacctccacc cccctgaacc aactggcgaa
    24661 aacatcctct cccagctcag cgcgtaaccg gctcttgacc ctcgcccaag cgtcgcttgg
    24721 cgcgccctgc tcctgttgtg ccacggttct tgctctttcc cattcttttg gttcgcgccg
    24781 ttacgcaccg cacgcctcat acatgagcag tcaacaagat cgacgcggac agtcatgacc
    24841 gtccctatga cttaccccaa accctagctt tgctgccagg gtaggccagc cgctttccac
    24901 cccgcaatgg ccccgcgatg cctgccgtcg tcgagcggtc cctcgaaacc gcaagcgacg
    24961 ttgtggcacg cgcggtatcc tatggccgcc atcgccttgg ccgcggccag gctccggctt
    25021 cccgaacggc aaatgaagaa gagttcgtct cccaaaccga caccaagcgc cttgagttcg
    25081 cccgccagac gctccgcaaa gcgcgtgtcg atcgcctgat cgggaaaggt ctgccactcg
    25141 acgaggacag gccgtttgcc aacaggcgcg aggtctggga ttcccacata ggtccactct
    25201 gcgcgcgttc gcacatcgac gagttgggat cgaggatggg attggagggt cgtccagacc
    25261 gtctccacat cgacatcgtc aaccgccgtc tcgttattct gtgtgctcaa aggaaacgcc
    25321 ctctcctgaa gacaagagta cgacgtaaga aacaaatttg gtgacgggta caataggtag
    25381 tggctacttg gcgaacaaga ccacgaccac ccacagatta cacgtcaatg ttccacaaga
    25441 tttccactct ccttgcaccg aagacaagaa aaacctctcc cggcaagagc caggtgaagg
    25501 tactcaattg tgaaggaggg gaacgccccg ttcggaatga ttggactata cacatgccca
    25561 atccgccgat caagcgagcg gcccccacgc acactgcaaa agcgcctcca tgacggggtg
    25621 ttcagcccaa tcgcacaagt ctctgtgtgg caagcgtgat tcatggaagc atgaagcgcg
    25681 gccgggccgg cgctgacgat ctctctcgaa cgcagcgtgg cacgaattct ttttgcaggc
    25741 gagtcgcaga tttcgcgcgt cgctcatcgc cgttttggcg cggatgagca agcagttgaa
    25801 atcgtcattg aatcgacaag agggaagagc ggtaagacgc gctctctgtg gccatcaagc
    25861 aacaggagcg gtttttttgt cagcgcgttc ccatcgcgcc gacccgcttc gcgagccgcg
    25921 acacttttcg cgatgccgcc ttgcgatggg cgacaccctt ctgggccccg cgcgcgatga
    25981 tgggctctgc ttccttcagg gcggcggcgg cggcctcctt gttgcctgag gcgattgcct
    26041 cctccacgtt gcgcatggag cttctgaggc gggtccgccg ggtcttgttg acggcggtgc
    26101 ggcgggtcgc ttgtcgagcc gccttttttg ccgatttcgt gttggccatg agatgatcct
    26161 cgaggcgcgc gcgacctcct ggtcaagact gcgctgctaa cgtgtgggct gatcggctgc
    26221 tctataacga gggcctcaag gagcgtcaac cgcgctaaga aagagcccgg cgcggcgccg
    26281 agggcgattg acagcgggaa aactcggtgg ctctcctgcg gcgcggcagg gagcggactg
    26341 ccggtcaaga aagcgaccca tgcgtgtttt ggttaccggc gccgccgggt tcataggcta
    26401 ttacacggcg gcgcggctgc tcgaccgtgg gcacgaggtc atcggcttcg acaatctcag
    26461 tccctattac gacgtgtcgt tgaaagaggc ccggcttcgg cgcctcaacg agcacgaggg
    26521 ctttcgcttc ctcaagggcg atctcgccga ccgggacgtc gtcgaggcga ctttcgccga
    26581 ggagcggccc gagcgcgttg ttcacctcgc cgcgcaggcc ggcgtccgct actcgctcga
    26641 ccacccgcac gcctatatca gctcgaacct gaccggcttt ctccacgtgc tcgagggctg
    26701 ccggcaccac ggggtcgagc atttgctcta tgcttcgacc agcgcggtct atggcgcgaa
    26761 ccagacgctc ccctttgccg tctgcgacaa tgtcgatcac ccggtgagcc tctacggcgc
    26821 gaccaagaag gcgaacgagc tgatggcgca ttcctacgcg catctgttcg gcatccccgt
    26881 gacgggcctg agattcttca ccgtctacgg gccgtggggc cggcccgaca tgtcgctctt
    26941 tctcttcacc cgcaacatcc tcgccggcga gccgatccag gtgttcaact acggccagca
    27001 ttcacgcgac ttcacctata tcgacgacgc cgtcgaaggc gtgctgcgca ccctcgatgt
    27061 cgtcgcgaca cccgatccga attggcgcgc cgcagctccc gatcccgcca cctcctcggc
    27121 gccctaccgg ctttacaata tcggcagtca caaccctgtc gccctgctcg attacgtcgc
    27181 ggcgatcgag aaggcgctcg ggcggaaggc aacgatcgaa ctgctgccgc agcagccggg
    27241 tgacgtcgta gcgacctatg ccgatgtcga ggcgcttcag gaggcgacgg gttttcagcc
    27301 ctcgacctcc ctcgaagagg gcatcgggcg ctttgtcgcc tggtatcgcg actattaccg
    27361 cgtgtgaggc cttgtccgcg ttgcgcttgc ccgccaaagc ggccctagcg ctggcaggtt
    27421 ggacaataga aactcgagcg tccgccttgc acgatgcggc gtatggttcc gccacagcct
    27481 ttcctagggc aaggcttgcc ttcgcggtcg taagtcctga atctgtgctg gaaacggccg
    27541 agcctcccgt cggcgcgctt atagtcgcgc agcgatgagc ctccggccgc aatcgcggct
    27601 tcgagcacgg ccctgaccga ggcgacgagc gcctccgtct ttttggtggg cttgcccgct
    27661 ttcgtggcga gagagcctgc cttgcgcaaa ggggagatgc cggcgcggtg caacgcctcg
    27721 caggcataga tgttgccgag gcctgcgatc agcgtctggt cgacgagcgc ggccttgatc
    27781 gaggtggtct tgcccttgag gcgctgcgcc atccattccg gcgtgaaggg ggggcttaga
    27841 ggctcgacgc cgaggcctct gaagagcgca tgatcatcga gcgcggcttc cggcacgagg
    27901 tccatgaacc cgaagcgccg cgcatcgtga tagcggagcg ccgtgccgtc gacgagatga
    27961 aacacgacat gctcatgttt tggcgtacgt tcctccgtgt atgcgaactc gccgagcacg
    28021 agaggggagt cttcgaagcc tcgagagacg cctcgagaga tgtcagtgcc atcgcgggaa
    28081 gggtgcttct tccgcggcgg gcaatcgatg ctgaagcgtc ccgtcatgcc gagatgcatc
    28141 accagcacct cgccgttatc gagccgggcg agcagatatt ttgcgcgccg cccgagcgcc
    28201 tcgactttcc gcccttcgag ccgctgcgcg aacgcgttcg gaagcggaaa gcgcaaatcg
    28261 gggcgcctct gctcgacgcg cgcaaacgcg ttgccgacga ggaacggcgc aagcccgcgg
    28321 cgcacggttt cgacctcggg gagttccggc atgacgatcc gctttgaacc aaagtacgat
    28381 ggaggcgaat tccgccgcga cgatagcctg ggcgtgggcc ttgcgctatt gtccgccccg
    28441 atgtcagagg acctgaaagg tcgcgccccc gacaacgcca agacgagctt cggcttcgcc
    28501 cgggtgggag agggggcgaa gcaaggcctt gtgaacgagg tcttcgccag ggtcgcgccg
    28561 cgctacgacg tgatgaacga tttgatgtcg ggcggccttc acaggctgtg gaaggacgac
    28621 ctcgtctcgt ggctcgcccc gccgaggagc gccgctcctt acgtccttct cgatgtcgcc
    28681 ggcggcaccg gggacgtggc tctcagggcg cttcatgcct cgggcccagg tgcctcggcc
    28741 gtgatttgcg acatcagcag cgagatgatc gaggccggac ggagacgggc agggggcgag
    28801 cggttgagct tcgtgcgcgg caacgccgaa gccttgccct tcgccaaagg gcggtttgat
    28861 gcctacacgg ttgcgttcgg catgcgcaat ctcacccatc tcgaagcggc gctcgccgaa
    28921 gccttccgcg tgctcaagcc cggcggccgc ttcctctgcc tcgagttttc cgccgtcgag
    28981 gtgccccttc tcgaccggct ctacgaggcc tattccttca atgcgatccc ggcgcttggg
    29041 cgtctcgttg ccggcgacag cgcgtcatac cgctatctcg tggaaagcat ccgccgcttc
    29101 cccgatcagg aaaatttcac gcatctgatc gagaatgcgg gcttcgagcg ggtgcgctat
    29161 cgcaacctga gcggcgggat tgccgcgatc cattccgcct ggcgtctatg atgaggctca
    29221 aatcgtgagc gcgattgcca atatggcacg tttgaccaag gcgggcgccg tgctcgcctg
    29281 gtcgggcgcg cgcgttcttc ctgagcaggc gaatctgccc gcgccgcttg cgctcttcgc
    29341 gcgcgtcacc gcgccgctgc ggaagaacgg accaggcgag ggtcggaacg agaccaagct
    29401 ctcggacgcg ctcacctcgc tcggaccgtc ctatatcaag ctcggacagt ttctcgcgac
    29461 ccgtgacgac atcatcggcc gcgagctcgc ccgcgatctg tcgaccctcc aggaccgctt
    29521 gccgccattt tcgcaagaag aggcgaggga agcggtcaag gaggctcttg gcgctccgat
    29581 cgacaagctc tatgccgagt ttgggccgcc ggtcgcagcc gcctcgatcg cgcaagtgca
    29641 caaggcgaag gtgcgcggtg ccgacggcac cctgacgccg gtcgccgtca aggtgctccg
    29701 gcccggcatc gagcagcgct tccagaagga cctcgacagc tatttcttcg ccgcgcggat
    29761 gatcgagcgg ctccatccac cttcgcggcg cttgcggccg atcgccgtgg tcgacacgct
    29821 ggccaagtcc gtcgccatcg agatggacct ccgcatggag gccgcggcca tctcggagat
    29881 ggctcagaac ataaagaacg atgagggctt ccgcgtgccg acggtcgatt ggacgagaag
    29941 cgcgcggcgc gtgctcacgc tcgaatggat cgacggcatt tcgattgcgg accgcgaagg
    30001 cctcgcagcc gccggtcacg atatcaaggg gctgggcccg cgggtgatgc aggccttcct
    30061 ccgccacgcc atgcgcgacg gcttcttcca cgccgacatg catccgggaa atctcttcgt
    30121 cgacgccgag ggccgcatcg tcgccgtcga tttcggcatc atgggacggc tcgggctgaa
    30181 ggagcggcgc ttcctcgccg agatcctgta cggcttcctg acccgcaact acacccgcgt
    30241 cgccgaggtc catttcaagg cgggctatgt gccgcgcaag cattccgtcg cgcagttcgc
    30301 ccaagcgctt cgcgccatcg gcgagccgat catggaccgg ccggccaacg agatctcgat
    30361 ggcgcaactg ctcggccagc tgttccaata taccgaagtg ttcgacatgc agacgcggcc
    30421 cgagcttctg ctcctgcaaa agaccatggt tgtggtcgaa ggcgtgggtc gcagcctcga
    30481 tccggagctc aatatctgga tcgtgtcgga gccggtggtg aaggcgtgga tggagcagga
    30541 gttcggcacc ggcgcccagt tcgaggcggc ggcgcaaggc gcggcctcgg tcgggcgcct
    30601 catgggcgac atcccgaggc tgctcggcca ggccgagcgc accgccgagg ccctttcggc
    30661 gatggccgag gagggtgtta ggctcgacga tcataccgtc gagcggctgg cggaggcgca
    30721 aggccgcgag aaccgggcaa gccgcatcgg catctggatc ggcgcgctcg cgcttatcgc
    30781 catcgcgctc tcattgctgt tctgatttcc ccgctctccc cttgggggag agcacgcgcg
    30841 cctcttggcg cgcggtgagg ggtaagcggg cgtaagcccg cgtcgagtaa aattcacccc
    30901 ctcacccttc ccgccattcg ccgcgcttcg cgcgtcgagt aaaattcaac ccctcaacct
    30961 tcccgccatt cgccgcgctt cgcgcgtcgg gcgggaagcc ctctccctca ggggggagag
    31021 ggggagtgct tgcattgctc ttctgagcgt tgaccgctaa ctctatttta ttgtcattgc
    31081 ccggaggccg aggttctatg gcttaggtcg cgccttgagc gtcataccgg cgaaagccgg
    31141 tatccagtgc caaaggcacc gtgcttgacc ctctggattc cggctttcgc cggaatgacg
    31201 agggggcggc aggaacgaga gtcgagacat gaacggtaag cgcatcctct tgatcgtcgg
    31261 cggcggcatc gccgcctata agagcctcga tctcgtgcgc cgcttgaagg agcggggtgc
    31321 cgcggtgcgc gccatcctca cgcgcggcgg cgccgagttc gtcacgccgc tttcgctcgc
    31381 cgtgctcacc gaggagaaga ccttcaccga tctcttcgac ctcaaggacg aggccgagat
    31441 cggccacatc ggtctctccc gcgaggccga tctcgtcgtc gttgcgccgg cgaccgccga
    31501 tcttctcgcc aagatggcga acgggttggc tgacgacctc gcctcgaccg tccttcttgc
    31561 caccgacaag aaggtgcttg ctgcgcccgc catgaatgtc cgcatgtggc accatcccgc
    31621 cacgcagcgg aacctcgcga tactccgcgc cgacggaatc ggctttgtcg gacccgacga
    31681 gggcgagatg gcttgcggcg aatatggact gggccgcatg gccgagcctc aagcgatcct
    31741 tgcggccatc gaagagatgc tcggcgcgga ggcgctcgcg ccacgcgccg acgccacgcc
    31801 gttgaggtca cttgccggct gccgcgtgct catcacctcc ggccccacgc aagaaccgat
    31861 cgatccggtg cgctacatcg ccaaccactc ctccggcaag cagggccatg ccttggcgcg
    31921 tgccgcgatc gcgctcggcg ccgaggtgac tctggtgacc ggccctgttg cgctccccga
    31981 ccctcaaggc gcgcgcgtgg tgaaagtaaa gaccgcggag gagatgcttg ccgcagtgct
    32041 cgcggcgctt cccgccgaca tcgcgatttg cgccgcagct attgccgatt ggcgcgcggc
    32101 gagcgaggcg cggcagaaac tcaagaaaga gggtggggaa gcttcgctga ggctcatgca
    32161 gaatcccgat atcctcgcga cgatcgcacg ccctgggccg aagcgcccgc gcctcgtcat
    32221 cgggtttgcc gcggagaccg agaacttgat cgccaacgca agcgagaagc gccgggccaa
    32281 gaacgccgac tggattgtcg ccaacgacgt ctcgtccggg aggggcgtga tgggcgccga
    32341 ccagacgcat gtccatctca tcacggcatc gggcgtggag gattggccgt ctctcagcaa
    32401 agacgagacg gcgacgcgcc tcctcgcccg cgccgccaac gcgctcgccg ccatgcgccc
    32461 ggcggcggaa tgagcgtcac cgttcgcgtg aaacggctcc cgcatggcga ggggctgccg
    32521 ctcccgtcct atcaatcagg agacgccgcg gggctcgatg tggtggccgg cgtgccggaa
    32581 ggctccccgc tcgtgcttct ggcgggcgcg cgcgccctca ttcccacggg cttcgcgctc
    32641 gaattgccgc gcggctacga ggcgcaagtc cgcccccgtt cggggctcgc gctgaagcac
    32701 ggggtgacgc tcctcaacgg cccgggcacc atcgattcgg actatcgcgg cgaactcatg
    32761 gtgattctca tcaatcacgg cgctgagccc ttcgtcctcc gtcgcggcga caggatcgcc
    32821 cagctcgtga tcgcgccggt cgctcgggtc gaaattgagg gcgccgatgc gcttgggacg
    32881 acggagcgcg ggcaaggcgg ctttggctcc accggctgac agaacttccg tttccggcgt
    32941 cgccctttcc cttttcccgc tctccccttg agggagagca cgcgccgctt gcggcgcggt
    33001 gaggggtaag cgggcgtaag cccgcgtcaa aaaaagattc accccctcac ccttcccgcc
    33061 attcgtcgcg cttcgcgcgt caggcggaaa gccctctccc tcaagggaga ggggaaagtc
    33121 ggctgcggcc tacctcactg gcggtgcggc cggggaaggc ataagttgcg catgatgctc
    33181 acccctgacg agatcgagcg ctacaagcgc cagctggtgc taaaggagat cggcggcgaa
    33241 ggccagcaga agctcaaagc ctctcgcgtg ctggtggtcg gcgcaggcgg gctcggatcg
    33301 ccgctcattc tttatctcgc cgccgccggg atcggcgaga tcgggatcat cgacagcgac
    33361 cgggtgagcc tcgacaatct ccatcgtcag atcgttcacg acgcgtcgag cgtcgggcga
    33421 tcgaagacga agagtgcggc ggccgcgatc gagcgcctca atcccaacgt cgcgacgcgc
    33481 ctctactcga cccgcctcgt ccctgagaac gcgcttggca tcatcggcgg ctacgacatc
    33541 gtcgccgacg gctccgacaa tttcgccacg cgctatctcg tcaacgacgc gtgctatttc
    33601 gcgaagaagc ctctcgtgtt cgcggccgtg ggtcccttcg acggtcacct caccacgttc
    33661 cgcgccttcg agaccgatcg cgcggggaat ccgcgcccgt cctatcgctg cctctttccc
    33721 gagccgccgc ctgacggcac tgtcgcgccc tgctcggaga ccgggattct cggccccgtc
    33781 gcgggcgtca tcggctctct tgccgcggtc gaagtggtca aggagctgct cgggatcggc
    33841 gagcgccttg tcggcaggct tctcctctac gacgcgcttc aagcacgctt cagcgcggtg
    33901 agctatggat gggaccccgg caatccgctg aacggccgcg ccccccgctt ccgtgatctc
    33961 tcgcatcatc gcccgatcct ggtaggcgca agcgagtgag ggacactggc ttaacttgct
    34021 cgtcataccg gcgaaggccg gtatccagca gatacgcttt tgaatctttc actggattcc
    34081 ggctttcgcc ggaatgacga aatagcggcg agtttggaga aaacatggcc accgagcaaa
    34141 tcgccacgag ctgcgccatt gccggcggcg ggcccgcggg catgatgctc gggtacctcc
    34201 tcgcgcgcgc cggcgtcgat gtcgtggtcc tggaaaagca cgccgacttc ctgcgcgatt
    34261 tccgtggcga cacgatccat ccctcgacgc tggaggtgat gcacgagctt ggcctgctgg
    34321 atgcgttcct caagctgccg caccagaagg tgagcgagat cggcgggatc gtcggcgacg
    34381 agatggtcac cttcgcagat ttcagccatc tcccgacgcg ctgcggcttc attgccttca
    34441 tcccgcaatg ggacttcctc gattttctcg ccgaccaggg caagcgctat ccgaccttca
    34501 agctcatgat gcaggccgag gtcgacgatc tcttgtggga aggggacaga gtggcaggac
    34561 tcaaagcgaa gctgcctgac cgcacgctcg agatcagcgc cggtctcgtc gtcggcgccg
    34621 atggccggca ctcgaaggtg cgcgatctcg cgggcctcga ggttgaggat ctcggcgcgc
    34681 cgatggacgt gctttggctc cggctctcaa agcgccctgg cgacggctcg cagaccctcg
    34741 gccgcatcca ggccggacgc cttttcgtca tgctcgatcg cggcgactac tggcagtgcg
    34801 ccttcgccat tcccaaaggc ggtttcgagg cgctgcgcgc caaagggatc gaagcgttcc
    34861 gcaacgagat cgtggcgctc aatccggcgc tcgcggaccg cgtgaaggag atcgcctcgt
    34921 gggacgacgt gaagctgctc accgtgcgcg tcgatcgcct gaaacgctgg tatcggcccg
    34981 ggctcttgtg catcggcgat gcggcccatg ccatgtcgcc ggtcggaggc gtgggcatca
    35041 acctcgccgt tcaggacgcg gtcgccgcag ccaatatcct atcggagccg ctgcgcatgg
    35101 ggcgggtcgc ggaggaggtg ctggccaagg tgcagaggcg gcgggaatgg ccgacaaaga
    35161 tgacgcaggc ggtgcaggtc ttcgtgcaaa ggcgtgtcat cagcaatgtg ctgagcatga
    35221 ccacgcagcc gcggccgccc tttgccgtca cgctgttcaa ccgcttcccc gttctccgcc
    35281 gtctgcctgc gcggctgatc ggcatgggtt ttcgcccaga gcatgtagag acgcccgttg
    35341 ctccagcgca aaaagaaatt cccctctccc cttgagggag agggcttccc gcccggcgcg
    35401 cgaagcgcga cgaatggcgg gaagggtgag gggggtcttc ttgacgcagg cttacgcccg
    35461 ctttccctca ccgcgcgctg cgcgcgcgtg ctctccctca aggggagcgg gaaaaacgag
    35521 actagtctag cgggagcgca ctcagaacat tgccggatgg acgcggtcgg gcggcgcgtg
    35581 accgtcgagg aaggtcttga tgttgatgat gaccttctcg cccatgtcga cgcggccttc
    35641 gagcgtggcc gagcccatat gcggcagcac caccactttg ctgctcttga gcagcttggg
    35701 gttgaccgcg ggctcgtgct cgaacacgtc gagaccggca ccggcaagct cgttgttctc
    35761 gatcatgcgg gcgagcgcgt tctcgtcgat cacctcgccg cgcgcagtgt tgacgatata
    35821 ggcggagggt ttcatcagcg cgagccggcg cgccgagagg agatgatagg tcgcgggcgt
    35881 gtgcgggcag ttgatcgaga tgatgtccat gcgcgcgagc atctggtcga ggctgtccca
    35941 ataggtggcc tcgagcgcct tctccacgtc gggcgtcacg cgccgcctgt tgtgatagtg
    36001 gatctggagg ccgaaggcct tggcgcgctt ggccacggcc tggccgatcc tccccatgcc
    36061 gacgatgccg agccgcttgc catagatgcg atggccgagc atccaggtcg gcgaccagcc
    36121 gtgccagcca ttggcctcgc tcttgagcag cgcggcgccc tcggtcagcc gccgcgggac
    36181 agctaggatc agcgccatgg tcatgtcggc ggtgtcctcg gtgagcacgc cgggcgtgtt
    36241 cgtgacgagg atcgagcggt tccgcgccgt ctcgag
//
LOCUS       DASUQP010000207        10742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_239446,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000207 DASUQP010000000
VERSION     DASUQP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10742
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239446"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1191
                     /locus_tag="VFY74_09235"
     CDS             232..1191
                     /locus_tag="VFY74_09235"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5931915.1"
                     /translation="MGLCAGQPFPILRSLAQNGQPSSDDFKAFQPGQFYWHPERAPKG
                     PVAIIVSVPKQLVFVYRNGILIGVSTCSTGKPGHSTPTGVFTILEKAKTHTSVKYGEP
                     MPDMQRLTWKGIALHVGGLPGYPSSHGCVHLPQDFADNLYAITQIGTPVIIAGGHNDP
                     VSVADPGPILGATAERQMASKAGEEMFSPSAPDAVTSILVSRADKKIYVLQNADIVAE
                     GEAIIVNPSEPLGSNVFVWQGGDAKGSTWAAMGFHADTTASIAPNGDVLQRIDGAPEV
                     MAAIRARIKPGTALVTTDLPATPDTRSDSNLVVIDSPSAEEAG"
     gene            1266..2288
                     /locus_tag="VFY74_09240"
     CDS             1266..2288
                     /locus_tag="VFY74_09240"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5931916.1"
                     /translation="MAKRGSSRRKVVAQLLLGAALLGCGNLIPITIARAARKKRKGGA
                     PATPAEEGKNLKPGQFTWHPERAPSGPVAIIVSVPKHCVYVYRNGILIGVSTCSTGKK
                     GFETPTGVFTILEKAKEHSSSEFEDAPMPNMERLTWGGIALHAGKLPGYPASHGCVRL
                     PAKFAENLYEVTHLGTPVIIAGDHTDPADVQDPGPVLGSSAKAEMDAKVGKKAAGGAN
                     GVTSILVSRADNRIYVLQGGAIAAEGSANIKDPSKPIGSNVFIWQDGDKNGSTWSGMG
                     FHVEADGAAKPNSAVLERIEGSDEVMDAIKKRLKPGTVLVTTDLAATPDTRTDKTMVV
                     MDGPAK"
     gene            complement(2295..3317)
                     /locus_tag="VFY74_09245"
     CDS             complement(2295..3317)
                     /locus_tag="VFY74_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX5931917.1"
                     /translation="MEGLSEQNDFAERLSVKIAAYHEAALIYAALKLGLPERLSARVW
                     TADALAAELGLSPPHLDRLLRGLSSLGICAECPDRTFALTPQGQCLLPGSPSRLWEKV
                     QVVVAQYWQPWANLAATIETGRPAFEHVFGTNVSDWRRDHPEDGALFDSYLAQETVAQ
                     APAIIAALDVSRVHTVADIGGGYGGLLAALLQTHSHLAGILFDRSHRIEAALPFLQSQ
                     GVAARVQRIGGDLLDTIPVEADLYLLNGVLQQWDDPHARAILANCREAMRNQARLAII
                     ERLLPEHAADDPAAIMLDLHMMAITGGRVRSVAEIGALLSDAGLTLAKATKTPSGLCV
                     IEAVPA"
     gene            3431..5038
                     /gene="guaA"
                     /locus_tag="VFY74_09250"
     CDS             3431..5038
                     /gene="guaA"
                     /locus_tag="VFY74_09250"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5931918.1"
                     /translation="MAEQIEVIDEARDTGRAEGLAQERVLIVDFGAQYTQLIARRVRE
                     AGVYSEIVPYDKAEEGLGPDLPKGIILSGGPASVHEAGTPQAPQALFSAGVPVLGICY
                     GEQAMVAALGGAVEGGHAREFGRAELQIIDDTPLFDGVFQVGERAPVWMSHGDRVTRL
                     PPGFRAVGISTGAPFAAVADDSRKLYGVQFHPEVVHTPKGAALLANFVHSIAGCAGDW
                     TMAHFRAREVERIRAQVGDGNVICGLSGGVDSAVAALLIHEAVGDQLTCIFVDHGLLR
                     QGEAEEVVDLFRGHYNIPLVAVDAGVRFLEALEGVTDPEAKRKTIGKLFVDVFEAEAD
                     KIGAVEFLAQGTLYPDVIESVSATGGPSATIKSHHNVGGLPERMRLKLVEPLRELFKD
                     EVRALGRELGLPDAFVKRHPFPGPGLAIRVPGEVTEEKLAILRKADAIYLDEIRRHGL
                     YDKIWQAFAVLLPVRTVGVMGDARTYDYVCALRAVTSTDGMTADYYDFSHEFLGAVAR
                     RIINEVRGINRVVYDITSKPPGTIEWE"
     gene            5337..5612
                     /locus_tag="VFY74_09255"
     CDS             5337..5612
                     /locus_tag="VFY74_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931919.1"
                     /translation="MRKEDALKILAAKLAEIEQEHESELAGNEGRREPIDAKAEARAL
                     TAIFDYLQNCKIVPTESLLRVFRRYLRATRPRAPMAEQGRSQRSKAS"
     gene            5686..6258
                     /locus_tag="VFY74_09260"
     CDS             5686..6258
                     /locus_tag="VFY74_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX5931920.1"
                     /translation="MTLLIIGIIAFLGVHALPTFPDLRTRLIGQWGETGYRIAFSLIS
                     IAAFVLLVYGFAKAPVVQVWSPPDWTRWVAIVLMLPAFIFLVAAYFPGRIKTSLKHPF
                     LVAIKTWALAHLIANGDLASIILFGSFLAYAVYDRIALKRRQPTGLVVAKGPAKAQND
                     VIAILAGTALYVIFLIWLHPLLIGQAVLPQ"
     gene            6346..7206
                     /gene="panB"
                     /locus_tag="VFY74_09265"
     CDS             6346..7206
                     /gene="panB"
                     /locus_tag="VFY74_09265"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5931921.1"
                     /translation="MAQDFALKRVTAPDIRNRKGGAPIVALTAYHAHTARIIDPHVDM
                     LLVGDSLGMVMHGYETTVPVPLELMIMHAQAVVRGSTRALVVVDMPFGSYEESPPVAF
                     RNAARVIKETGCGAIKIEGGLQMAETIRYLSERGVPVMSHIGMTPQAINTIGSFSPRG
                     RRRSEWAAFEADAKAVAEAGAFAVVLEALAELLAARITKLIDIPTIGIGASVECDGQI
                     LVLEDMLGLSPRVPKFVKEYAKLGAGIESAVKAFASEVRARAFPATEHTYPMADEDAE
                     VTKKTKMTGS"
     gene            7374..8054
                     /locus_tag="VFY74_09270"
     CDS             7374..8054
                     /locus_tag="VFY74_09270"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5931922.1"
                     /translation="MSDRDFFREVDEAVRQDKYRKLWDQYGVYVLGAAAIMVAGVAGF
                     QGWSFWQEKRAAEAGAKFSQAESIENGADAARAREMFGALAEQGPAGYRVLARFQLAA
                     AAAKAGETDKAVADYDALAKDASVDSILQGHATLQAASLRLDKADYAEMEQRLRGLAE
                     SDSAWRFSARELLGFSAYRLKNMREAEKQFNALIGDEATPPNLRERANMMIALIVGAP
                     QTLSTTAR"
     gene            8083..9438
                     /locus_tag="VFY74_09275"
     CDS             8083..9438
                     /locus_tag="VFY74_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5931923.1"
                     /translation="MRSGRKRGFGDFLLGAGLVAALALGLAGCGGSTLETLERKIKNP
                     FAKKEKPLPGERIAVITDPTAVVADPAEAARAVQLPAPQANASWSQPGGSPSNSLGHL
                     ALNGDVRKVWSADAGTGSSSSGRLSAVPLVADGKVFTLDAGGTVSAFAAASGGRLWSA
                     DVTPDNEKSKEGFGGGLALDGGRLYAATGFGTVVAIDTGNGAVAWSKRVGEPIRSAPT
                     AAGGKIYFVSAGNILYALNADDGEQLWTSRGLPQSATLLSNVSPAVSGGVVVAPFPAG
                     DVAAFEVGSGKATWRDSLARTTETMASAVLGDPARPVIDRGVVFAVSHGGKMIAVSES
                     SGERLWTRNLASTQMPWVAGDTVYVVDLAGKLIALSRADGKVRWVSDLPSGARWSGPV
                     LAGGKLWAVSGNGLLVGADARTGQLLSQLDLDTPVFVAPVVAGGRMYILADNASLIAL
                     N"
     gene            9487..>10742
                     /gene="der"
                     /locus_tag="VFY74_09280"
     CDS             9487..>10742
                     /gene="der"
                     /locus_tag="VFY74_09280"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5931924.1"
                     /translation="MTFTVAIVGRPNVGKSTLFNRLAGRKLALVDDQPGLTRDRREAE
                     ATLGACTVRLIDTAGFEPGDRGLTARMRKQTEAAIASADLVLFLIDARAGLVPADEIF
                     AELVRKSGKAVILAVNKSEGRAAEPAIYEAYALGLGEPIPISAEHDIGIGELAEAIEQ
                     ACREGSGVAAEEGEEPAEAPPHPLRIAIVGRPNVGKSTLINTLLGEERMITGPEAGIT
                     RDAIASGLAWNGRSLLLFDTAGLRRKMRVEGRAEELSVGDALKAIRFAEIVVLLLDAE
                     HPFEKQDLQIADLIAQEGRALVIAVNKWDLVREPGKRLAALRETCERLLPQVRGVTLV
                     PISATAGTGLDKLMKAILAADALWNQRLPTHALNDWLLAAVEAHPPPAVSGRRIKLRY
                     ITQANARPPTFVLFCPRPKALPDSYAR"
BASE COUNT         1904 a         3439 c         3534 g         1865 t
ORIGIN      
        1 atttctacgc accgggcgac ggcaagattg ttttcgtgaa ggatcttgct gccgatacac
       61 agcctccgcg tcaaacacca tcaggcgagc cattttcgtc tcggtaagga cggtggagag
      121 gcgtcgttga cattcttgtg gcgggcgtag gatcgtcacg cgacgggagg taggcatcga
      181 gagcctgcgg ggctcacccg gcgctcaacc gtcgccctgc tcggcctggg gatcggactt
      241 tgtgccggtc agccttttcc catccttcgt tcactcgccc agaacggcca gccttccagc
      301 gacgacttca aagcttttca gccggggcag ttctattggc accccgagcg ggcgcccaag
      361 gggcccgtgg ccatcatcgt ctcggtgccg aagcagctcg tgttcgttta ccgcaacggc
      421 atcctgatcg gcgtctccac ctgctcgacg ggcaaaccgg gtcacagcac gccaaccggg
      481 gtgtttacga tcttggagaa ggccaagacc catacctcgg tcaagtatgg cgagccgatg
      541 ccggacatgc agcggctgac gtggaagggc atcgcgctcc atgtcggcgg cttgccgggc
      601 tacccgtcgt cgcatgggtg cgttcatctc ccgcaggact ttgccgacaa tctctatgcg
      661 atcacgcaga tcggcacgcc ggtgatcatt gccggaggcc ataacgatcc ggtgtcggtg
      721 gccgatcccg gccccattct cggtgcgacc gccgagagac agatggcgtc gaaggcggga
      781 gaggagatgt tctcgccgtc agcccccgat gcggtcacct cgatcctcgt gtcgcgggcc
      841 gacaagaaaa tctacgtgct ccagaatgcc gatattgttg ccgaggggga ggcgatcatc
      901 gtgaacccat ccgaaccgct cggctccaat gtctttgtct ggcagggcgg cgacgccaag
      961 ggctcgacct gggcagcgat gggtttccat gccgatacga cggcttccat cgcgccgaac
     1021 ggcgacgtgc ttcaacgcat cgacggcgcg cccgaggtta tggccgcgat cagggcgcgg
     1081 atcaagcctg ggaccgcgct cgtcaccacg gacttgccgg cgacgcccga tacgcgaagc
     1141 gacagcaatc tcgtcgtgat agacagtccg agcgccgagg aggcgggtta gcggtaagaa
     1201 tcgcgcttgc gctggaaatg acacggactt agagggtcga aaccgccttc ccaaggagga
     1261 tcgccatggc aaagcgaggt tcgagccggc ggaaagtcgt ggcgcagctc ctgctcggcg
     1321 ccgccttgct cggctgcggc aatctcattc cgatcacgat cgcgcgcgcc gcgcgaaaga
     1381 aacggaaggg cggtgccccc gccacgccgg cggaagaggg caagaacctc aagcccggtc
     1441 aattcacctg gcatcccgaa cgcgcgccga gtggccctgt cgccatcatt gtctcggtgc
     1501 ccaagcattg cgtctatgtc tatcgaaacg ggattctgat cggtgtgtcg acctgctcga
     1561 cgggaaagaa ggggttcgag acgccgaccg gcgtcttcac cattttggag aaagcgaaag
     1621 agcactcctc aagcgagttt gaggatgcgc cgatgccaaa tatggagcgc ctgacctggg
     1681 gcggcatagc gcttcatgcg ggcaagctcc ccggctaccc ggcaagtcat ggctgcgttc
     1741 gtcttccggc aaaattcgcg gagaatctgt acgaggtgac gcatctcgga acgcccgtca
     1801 tcattgctgg cgaccacacc gatccggcgg acgtgcagga tcccgggccc gttctcggtt
     1861 caagtgcgaa ggcggagatg gacgccaaag tcggcaagaa ggcggcgggc ggcgccaacg
     1921 gcgtcacctc gatcctggtg tcgcgtgccg acaatcgcat ctacgttttg cagggcggcg
     1981 cgatcgctgc cgagggaagc gccaatatca aagacccgtc gaagccgatc ggctcgaacg
     2041 tgttcatctg gcaggacggc gacaagaacg gctcgacctg gtcaggcatg ggcttccacg
     2101 tcgaggccga cggtgccgcc aagccgaaca gcgccgtgct cgagcgcatc gagggctctg
     2161 acgaggtgat ggacgcgatc aagaagcggc tgaagcccgg caccgttctg gtcacgaccg
     2221 atcttgcggc cacgcccgac acgcgcaccg acaagaccat ggtggtgatg gacggcccgg
     2281 ccaagtgagc cggcttacgc cggcacggcc tcgatcacgc aaagccccga cggcgtcttc
     2341 gtcgctttcg cgagcgtaag gccggcgtcg gacaagagcg cgccgatctc ggcaacactc
     2401 cgcacccggc cgccggtgat cgccatcatg tgaaggtcga gcatgatcgc ggcggggtcg
     2461 tccgcggcat gctccggcag aagccgctcg atgatggcga ggcgtgcctg attgcgcatc
     2521 gcctcgcggc agttcgcgag gattgcgcgt gcgtgcggat cgtcccattg ctggagcacg
     2581 ccgttcagca gatagagatc ggcctccaca ggaattgtat cgagaagatc gccgccgatg
     2641 cgttggacgc gggcggcgac gccctgcgac tgcaagaatg gcagcgccgc ctcgatccga
     2701 tgcgaacgat cgaacaggat gccggcaaga tgcgagtggg tttgcagaag cgcggcgagc
     2761 agtccgccat aaccgccgcc gatatcggcg accgtgtgta cgcgagaaac gtcgagcgcc
     2821 gcgatgatcg cgggagcctg agccaccgtc tcttgcgcca aataggagtc gaataacgcg
     2881 ccgtcttcgg ggtggtcgcg gcgccagtcc gagacgttgg tgccaaagac gtgctcgaaa
     2941 gcgggtctgc ccgtttcgat cgtcgcggcg agattggccc aaggctgcca atattgggcg
     3001 acgacgacct gaactttttc ccaaagccgc gagggcgagc ctggaaggag gcattggcct
     3061 tgaggcgtga gggcgaaggt cctatcggga cattcggcgc aaatgccgag gctcgacagt
     3121 ccgcgcaaaa ggcggtcgag atgcggcggc gacaggccga gctcggccgc cagcgcatct
     3181 gccgtccaga cccgcgcgct caatctttcg ggcaagccga gcttcagcgc ggcatagatc
     3241 agcgccgctt cgtgataggc ggcgatcttg acgctcagcc gctcagcaaa gtcattttgc
     3301 tcggaaagcc cttccatcat gccacccccg atgacccttc cggccggcgt ggcgagggta
     3361 ttggtttaaa cccggaatga cgagacggag aatcattgcc tggtcgaagg cgctgctata
     3421 accacccgcc atggcagagc agatcgaagt catcgatgag gcccgagata ccggcagggc
     3481 cgaggggctc gcgcaggagc gcgtgctcat cgtcgatttc ggcgcgcaat atacccagct
     3541 cattgccagg cgcgtgcgcg aggcgggcgt ttattcggag atcgttccct acgacaaggc
     3601 ggaagagggg ctcgggcctg acctgcccaa ggggatcatc ctctccggcg gcccggcaag
     3661 cgtgcacgaa gcaggcacgc cgcaggcccc ccaggccctc ttctccgccg gcgtgccggt
     3721 gctcggcatc tgctatggcg agcaggccat ggtggcggcg ctcggcggcg cggtcgaggg
     3781 ggggcatgcg cgcgagttcg gccgcgccga gcttcagatc atcgacgaca cgccgctctt
     3841 cgacggcgtg tttcaagtcg gcgagcgggc gccggtgtgg atgagccacg gcgatcgcgt
     3901 gacgcgcctt cctccgggct ttcgcgccgt cggcatctcg accggcgcgc cgttcgcggc
     3961 ggtcgccgac gattcgcgca agctctatgg cgtccagttc catcccgaag tggtgcacac
     4021 gccgaagggc gcggcgctgc tcgccaattt cgtccattcg atcgcgggct gcgccggcga
     4081 ctggaccatg gcgcatttcc gcgcgcgcga ggtcgaacgc atccgcgcgc aagtcggcga
     4141 cggcaatgtc atctgcgggc tttccggcgg cgtcgattcc gccgtcgccg cgctcctgat
     4201 tcacgaggcg gtcggcgacc agctcacctg catcttcgtc gatcacggcc tgttgcgcca
     4261 aggcgaggcg gaagaggtcg tcgatctctt ccgcggtcac tacaacatcc cgctcgtcgc
     4321 ggtcgatgcc ggcgtgcgtt tcctcgaggc cctggagggc gtcaccgatc cggaagcgaa
     4381 gcgcaagacg atcggcaagc tcttcgtcga tgtgttcgag gcagaagccg acaagatcgg
     4441 cgcggtcgag ttcctggcgc aaggcacgct ctatcccgac gtgatcgaga gcgtgtcagc
     4501 gacgggaggt ccatcggcca ccatcaagtc gcatcacaat gtcggcgggc tgcccgagcg
     4561 catgcgactc aaactggtcg agccattgcg cgagctgttc aaggacgagg tccgcgcgct
     4621 cgggcgcgaa cttggactgc cagacgcttt cgtgaaacgc catccgttcc cgggacccgg
     4681 acttgccatt cgcgtgccgg gcgaagtcac cgaggagaag ctcgcgatcc tgcgcaaagc
     4741 cgacgcgatc tatctcgacg agatcaggcg gcacgggctc tacgacaaga tctggcaggc
     4801 cttcgccgtg ctgcttccgg tgcgcaccgt gggcgtgatg ggcgatgcgc gcacgtacga
     4861 ctatgtctgc gcgctccggg ccgtgacctc gaccgatggc atgacggccg attactacga
     4921 cttctcccac gaattcctgg gcgccgtggc gcgccgcatc atcaacgagg tgcgcggcat
     4981 caaccgcgtg gtctacgaca tcacctcaaa accgcccggc accatcgagt gggagtgatt
     5041 ccggcgagca aggcccgcga agactcgccg gtggggagga tttccctctc cccttgaggg
     5101 agagggcttc gcgcccgacg cgcgaagcgc ggcgaatggc gggaagggtg aggggtgaac
     5161 ctttttgacg cgggcttacg cccgcttacc cctcaccgcg agcgcgaaaa atgcgctcgc
     5221 gtgctctccc tcaaggggag agcgggaaaa tgagaggcat cttttcacgc cagttcgggc
     5281 tccgaactcg cttataatgc cgcaggggcg acagatgtgg ctgcttgggt ggcgtgatgc
     5341 gcaaagagga cgctctcaag atcttggcgg cgaaactcgc cgagatcgag caggagcatg
     5401 agtcggaact tgcagggaat gaaggccgtc gcgagccgat cgacgcgaag gccgaggcgc
     5461 gcgcgctgac cgccatcttc gattacctac agaactgcaa aatcgtgcca accgagtcgt
     5521 tgcttcgcgt gttccggcgc tatttgcgcg ctacgcgccc ccgcgctccc atggctgaac
     5581 aaggccggtc ccagaggtcg aaggcgagct gaagggctcg gcagttcgcc cgatcgatgg
     5641 gcgaatcggc tagaacatcg cctcaagcac aaacggcaga catccatgac cctcctcatc
     5701 atcggcatca tcgcctttct cggcgtccac gctttgccaa cgttcccgga cttacggacg
     5761 cggctgattg gccaatgggg cgagacggga tacagaatcg ccttttcgct catttcgatc
     5821 gcggcctttg tcctcctggt ctacggcttc gccaaggcgc cggtcgtgca ggtctggtcg
     5881 ccgccagact ggacgcgctg ggtggcgatc gtgctgatgc tgcccgcctt catcttcctg
     5941 gtcgcggcct actttccggg gcggatcaag acctcgctca aacatccctt cctcgtcgcg
     6001 atcaagacct gggcgctcgc ccatctcatc gccaatggcg acctcgcctc catcatcctt
     6061 ttcggctcgt tcctggctta tgccgtctac gaccgcatcg cgctgaaaag gcgccagccg
     6121 acgggtttgg tcgtggcgaa ggggccggca aaggcgcaaa acgacgtcat cgccatcctg
     6181 gccgggaccg cgctctacgt catctttttg atctggctgc acccgctcct gatcgggcag
     6241 gcggtgctgc ctcaatgagg gcaggcccgc gacttttttg cactgcaaaa ctccccatat
     6301 ttctgccata actgagcggg tttggcttaa aggagccgag cggaattggc ccaggacttt
     6361 gccttgaagc gggtgaccgc accggacatc cgcaaccgca agggcggcgc cccgatcgtg
     6421 gcgctcaccg cctatcacgc ccataccgcg cgcatcatcg acccgcatgt cgatatgctg
     6481 ctcgtcggcg attccctcgg catggtcatg cacggctacg aaacgacggt gcccgtgccg
     6541 cttgagctga tgatcatgca tgcgcaggcg gtggtgcgcg gctcgacccg ggcgctcgtc
     6601 gtggtcgaca tgccgttcgg ctcctatgag gagagcccgc cggtcgcctt ccgcaatgcc
     6661 gcccgcgtga tcaaggaaac gggttgcggc gccatcaaga tcgaaggcgg tctgcagatg
     6721 gcggagacca tccgctatct gtccgagcgc ggcgtgcccg tcatgagcca tatcggcatg
     6781 acgccacagg ccatcaacac gatcggcagc ttctcgccgc gcggccggcg gcgttccgag
     6841 tgggcggcgt tcgaggcgga tgccaaagcc gtcgccgaag ccggcgcctt cgctgtcgtg
     6901 ctcgaagcac tcgcggagct tctcgccgcg cgcatcacca agctgatcga cattccgacc
     6961 atcggcatcg gcgcttcggt ggaatgcgac ggccagatcc tggtgctcga ggacatgctc
     7021 ggtctctcgc cccgcgtgcc gaaattcgtc aaggaatatg cgaagctcgg cgccggcatc
     7081 gagtccgccg tcaaggcctt cgcaagcgag gtccgcgccc gcgccttccc tgccactgaa
     7141 catacctatc cgatggcgga cgaggacgcc gaagtcacga aaaagaccaa aatgacaggc
     7201 agttagccct cattcgggct tcgtttggcg gtccacgggg gaacgctgtc tccctttaac
     7261 ttgcgtggcc ggggcgcctt cactatacgg cggcaggagg aacggttcgc ggcccggaag
     7321 gccttcccgc ctcgtttcgc cacaaatgca aagcaagaag agtccgctgc cccatgagcg
     7381 accgtgattt ctttcgcgag gtggacgaag ccgtccgcca agacaaatac aggaagctgt
     7441 gggaccagta cggcgtctat gttctgggcg ctgcggcgat catggtcgcg ggcgtcgcgg
     7501 gcttccaggg ctggtcgttt tggcaggaga agagggccgc cgaggcggga gccaagttct
     7561 ctcaagccga aagcattgag aatggcgccg acgcggcgag ggcgcgcgag atgttcggcg
     7621 ccttggccga gcaggggccg gccgggtacc gcgtgctggc gcggttccag ctcgcggcgg
     7681 ctgcggccaa agccggtgaa accgacaagg ccgtcgccga ttatgacgcg ctcgccaagg
     7741 atgcgagcgt cgattccatc ctgcaaggcc acgccacgct tcaggcggcc tcgctcaggt
     7801 tggacaaggc ggattacgcc gagatggagc agcgtctcag gggtcttgcc gagtccgata
     7861 gcgcctggcg cttctctgca cgtgagctgt tggggttttc ggcctatcgg ctgaaaaaca
     7921 tgcgcgaggc cgaaaagcaa ttcaatgcgc tgatcggcga cgaggcgact ccgccgaatt
     7981 tgcgggaacg tgccaatatg atgatcgcgc tgatcgttgg agcgccgcag acattgagca
     8041 ccacggcaag atagctggcg cgtaacgaag ggtggctcga agttgcgctc gggtcgaaaa
     8101 cggggatttg gcgattttct gctcggcgcc gggctcgtgg ccgccttggc cctcggtctt
     8161 gccggatgtg ggggcagtac gctcgagacc cttgagagaa agatcaaaaa cccgttcgca
     8221 aagaaagaaa aaccccttcc gggcgaacgg attgccgtca tcaccgatcc gactgcggtc
     8281 gtggccgatc cggcagaggc ggcaagggcg gtccagctcc ccgcgcccca ggccaatgcg
     8341 tcatggtccc agcccggcgg atcgccctcc aacagtctcg gccacctcgc gctgaacggt
     8401 gacgtccgca aagtgtggtc cgccgatgcc ggaaccggct cctcgtccag cggacgcttg
     8461 agcgcggtcc cgctcgtggc cgacggcaag gtcttcacgc tcgacgccgg cgggacggtg
     8521 tcggccttcg ccgcggcgag tggaggccgg ctctggtccg ccgacgtgac ccccgacaac
     8581 gagaagagca aagagggctt cggcggcggc cttgccctcg acggagggcg gctctatgcc
     8641 gcgaccggct tcggcaccgt cgttgccatc gataccggca atggcgcggt ggcgtggtcc
     8701 aagcgtgtcg gcgaaccgat ccgcagcgcg ccgacggccg ccggcggcaa gatctatttc
     8761 gtctcggccg gcaacattct ctatgccctc aacgccgatg atggcgagca gctttggacc
     8821 tcgcgcggcc tgccgcaatc ggcgacgctt ctcagcaatg tcagccccgc ggtgagcggt
     8881 ggtgtcgtgg ttgcgccctt tccggccggc gacgtcgccg ccttcgaggt cggcagcggc
     8941 aaggcgacat ggcgcgattc gctcgcgcgc acgaccgaga ccatggcttc cgccgtcctc
     9001 ggcgatccgg cgcgcccggt catcgatcgc ggagtggtgt tcgcggtcag ccatggcggc
     9061 aagatgatcg cggtttccga atcgtccggc gagcggttat ggacgcgaaa cctcgccagc
     9121 acgcagatgc cgtgggtcgc gggcgacacc gtctatgtgg tcgatctcgc cggcaagctc
     9181 atcgcgcttt cgcgtgccga cggcaaggtg cgatgggtgt cggatcttcc gagcggcgcg
     9241 cgctggagcg ggccggtgct cgccggcgga aagctctggg cggtctccgg gaacggcctt
     9301 ctcgtgggcg ccgatgcgcg gacgggccag ctcctctccc aactcgatct cgacacgccg
     9361 gtcttcgtgg cccctgttgt cgccggcgga cgcatgtata tcctcgccga caacgcctcg
     9421 ctgatcgccc tcaactagcc tccctcgggt cctcgcgacc ccgcaacgtc cgcaagtcac
     9481 cttccgatga ccttcaccgt cgccattgtt ggacggccga atgtcggcaa gtccaccctg
     9541 ttcaaccggc tggcgggaag gaagctcgcg ctcgtcgacg accagccggg cctgacgcgc
     9601 gaccggcgtg aggccgaggc gacgcttggc gcctgtacgg tgcggctgat cgatactgcg
     9661 ggattcgagc cgggggatcg cggactgacg gcgcgcatgc gcaagcagac ggaagcggcc
     9721 atcgccagcg ccgatctcgt gctgttcctt atcgatgcga gggcgggctt ggtgccagcc
     9781 gacgagattt tcgccgagct cgtgcggaag tcgggcaagg ctgtcattct tgcggtcaac
     9841 aagagcgagg ggcgcgcggc cgagcctgcg atttacgaag cctatgcgct cggcctcggc
     9901 gagccgatcc cgatctcggc cgaacacgac atcggcatcg gcgaactcgc cgaggcgatc
     9961 gagcaggcgt gccgggaagg gtccggtgtt gcggctgaag agggcgagga accggccgag
    10021 gcgccgccgc atcccttgcg gattgcgatc gtggggcggc cgaatgtcgg caagtcgacc
    10081 ctcatcaata ctctcctcgg cgaggaacgc atgatcacgg gcccggaagc cggcattacg
    10141 cgtgacgcca ttgcctccgg ccttgcatgg aacggccgct ccttgcttct cttcgacacg
    10201 gcggggctcc ggcgcaagat gcgtgtcgaa gggcgcgccg aggagctgtc ggttggcgac
    10261 gcgctcaagg cgatccgttt cgccgaaatc gtcgttctgc ttctcgacgc cgagcacccc
    10321 ttcgagaagc aagatcttca gatcgcggac ctgatcgcgc aggaagggcg ggctctcgtc
    10381 atcgccgtca acaaatggga tctcgtgcgc gagcctggaa agcgtcttgc tgcgctgcgc
    10441 gagacgtgcg agcggctcct gccgcaagtc agaggcgtga cgctggttcc catttcggcg
    10501 acggccggaa ccggcctcga caagctgatg aaggcaatcc tcgccgcgga tgccttgtgg
    10561 aaccagaggc ttcccacgca cgcgctcaac gactggctgc tagccgcagt cgaagcgcac
    10621 ccgcctccgg ccgtctccgg ccggcgcatc aagctcagat atatcaccca ggccaatgcg
    10681 cggccgccga ccttcgttct tttctgcccg cgcccgaagg cgctgcccga ctcttatgcg
    10741 cg
//
LOCUS       DASUQP010000208        16098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_648605,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000208 DASUQP010000000
VERSION     DASUQP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648605"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFY74_09285"
     CDS             complement(<1..332)
                     /locus_tag="VFY74_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931925.1"
                     /translation="MIISHEHKFIFLKTKKTAGTSIELALSELCGPDDIIAPLTKIDE
                     ALRASARGAQNWRLHGWWQSPRPFWQRRFFKFAAEDYGFYNHMPAEQAKTLFNDDRAW
                     RSYFKFAF"
     gene            complement(580..1041)
                     /locus_tag="VFY74_09290"
     CDS             complement(580..1041)
                     /locus_tag="VFY74_09290"
                     /inference="COORDINATES: protein motif:HMM:NF016291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HEX5931926.1"
                     /translation="MASAAHNRGPCAEPWGAEMGDKLATQDNDWVLKAMVAVAASDGG
                     LDARETGLIQKVYQDQSGHALSADEVARAADALAKGDAIAEFAAAAKTLDRGAKEEVI
                     KGAYRVLLADNRIAGEERKKLKDIAAALQIPEIHFGAILEDLAIWLAKEPR"
     gene            1205..1888
                     /locus_tag="VFY74_09295"
     CDS             1205..1888
                     /locus_tag="VFY74_09295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5931927.1"
                     /translation="MRVGRLIPPGRYARKMAREMRILVLPHLFDRYELRLLSRVMHPG
                     SVFIDVGANVGIYTVFAALRGGPTSRVFAVEPNPAALERLHCNLSLNGLTNVVVEPIA
                     LGEKAGLVSFKLDRRNIGNSSLVFDRDAPAGDLIEVQGQTLMDLCARYGIVRLDAIKL
                     DVEGAEDQILFPFFAEAPASLWPTLLLVENSEGQWRRDCASMLIEKGYRRKRIPSRNL
                     AFWRPAPRP"
     gene            1906..2952
                     /locus_tag="VFY74_09300"
     CDS             1906..2952
                     /locus_tag="VFY74_09300"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HEX5931928.1"
                     /translation="MKPLRVVFLADTRPGHFHVAQGVIAALERLRPVEVTRIEVKRKW
                     IVPTRWLRRRITAKTFFPPRMLRMAYRIDAYALPKADLVVSAGGETQMPNICVARFLD
                     IPSIFCGSLLRGLSPENFSLIISSYERDATSPRHLVVLKPSSIDPEALGRPKIVPRYG
                     PGKRPELAGLLIGGNAGPFRYRRAEWERLLAFAGDVSRAWGTRWLVSTSRRTPDFVAD
                     RVAELAANSGIVSRFIDYRKAGPGTLPEVFSQAEVIVCTEDSSTMISEAVCARLPVVG
                     AAPSAHRFTDEEQEYRDFLVRNDWCRVLPIAELTPDTFARALSEIEVLQENPLDALAA
                     KLKERLPDLLGDRD"
     gene            3014..4672
                     /gene="ettA"
                     /locus_tag="VFY74_09305"
     CDS             3014..4672
                     /gene="ettA"
                     /locus_tag="VFY74_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX5931929.1"
                     /translation="MATPQYVYVMNRLSKTYQGGKQVLKDVTLAFLPGAKIGVVGVNG
                     SGKSTLLRIMAGQETEFVGEAWAAEGVSVGYLPQEPVLDETKDVAGNVMEGVAGKKAK
                     LDRYNELAMNYSDETTEEMAQLQDEIDAQGLWDLDAQVEQAMDALCCPPGDTDVKTLS
                     GGERRRVALCRLLLAKPDILLLDEPTNHLDAESVAWLEHHLRDYPGTVIMVTHDRYFL
                     DNITEWTLELDRGAGIPYKGSYSSWLEQKKMRLDVEGKQDEARQQTLARELDWIRASP
                     KARQAKSKARIRAYEDMLAEAARDKGGKAQITIPPGPRLGDVVIEADQLNKGFGDMLL
                     IDELSFKLPPGGIVGVIGPNGAGKTTLFRMIVGQDKPDDGTIRVGETVKLGYVDQSRD
                     ALDGRKSVFEEISGGNDVIKLGTRDVPSRAYVGWFNFKGSDQQKKVGQLSGGERNRVH
                     LAKMLKSGANLILLDEPTNDLDVETLSSLEAALEDFPGCAVIISHDRFFLDRIATHIL
                     SFEGDSHVEWFEGNFEDYEEDKKRRLGEDAVLPKRIKYKHFARR"
     gene            4719..5747
                     /locus_tag="VFY74_09310"
     CDS             4719..5747
                     /locus_tag="VFY74_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5931930.1"
                     /translation="MSSDEKSGPPGLSRAQALAGLRAAGEATRLRILALLSAGELNVK
                     DLTQILGQSQPRISRHLKLMAEAGLIRRFREGSWVFFRLADSGAEAALARAIVASLDP
                     TDLTLARDRARASAVEKTRAEAAQAYFKTHASEWDEIRALHVAEKDVEAAMDAALGRD
                     PFGLLVDLGTGTGRILELFASRATRALGFDLNHAMLAYARMKLERAGLSHAQVRHGDL
                     NNVPLPDGAADAVVLHQVLHFLDDPAAAIAEAGRLLAPGGRLLVVDFAPHELEFLREQ
                     SAHRRLGFGRAQLGRLFESAGLKLERFRELKPAPSGGKLTVSLWLGLKPAAAAKKRKV
                     GAKVEAAA"
     gene            5747..6667
                     /gene="metF"
                     /locus_tag="VFY74_09315"
     CDS             5747..6667
                     /gene="metF"
                     /locus_tag="VFY74_09315"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX5931931.1"
                     /translation="MLASSEPKSRILGRGAIDVSFEFFPPKTEAMEQTLWSSIRRLEP
                     LRPRYVSVTYGAGGSTRERTHGTVARILRETTLKPAAHLTCVEATREEIDDIIRDYWD
                     LGVRHIVALRGDPPGGVGQRYQPTPGGYGNAAGLAEGIKAIAPFEVSVGCYPEKHPES
                     PSVEADIDVLKAKIDAGATTAITQFFFDNAMYFRYLDRVRARGITIPIVPGIVPIHNF
                     KQIAIFAAKSGASVPDWLGHRFEGLDDDQETRHLVAAAVAAEQVLGLVDQGIKEFHFY
                     TLNRADLVYAICHLLGLRPAKAFLDVKAAS"
     gene            6664..10413
                     /gene="metH"
                     /locus_tag="VFY74_09320"
                     /pseudo
     CDS             6664..10413
                     /gene="metH"
                     /locus_tag="VFY74_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            10927..11211
                     /locus_tag="VFY74_09325"
     CDS             10927..11211
                     /locus_tag="VFY74_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931932.1"
                     /translation="MKTVTASGLGLLGLLIALPAQALTITNTDSDPRTVTVKAGSDNK
                     ALTVEAGKAVEPPCDPSCTIELENGELYEMQGDEVVAIEDGIIFVDAVTP"
     gene            complement(11262..11933)
                     /locus_tag="VFY74_09330"
     CDS             complement(11262..11933)
                     /locus_tag="VFY74_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX5931933.1"
                     /translation="MAGSPAAAEEKAPSPCTEDAMIVFDASGSMSGNQTLGIPNSRPR
                     IDEVRAALARVLPTATKHRRVGLITYGPGPYNQCNVKLDLKPTADAFKQIMRAVNALV
                     PAGKTPLTSAVQQAAEVLNYRNKPGVIVVVTDGEETCGGLPCELGKQLRLLGHQLTVH
                     AIAFRYEGFSWTGGNSVVDLMCLANENRGLFARANSEQDLVDALEKTLDCPMISQTGP
                     VPITR"
     gene            complement(12110..13015)
                     /gene="rimK"
                     /locus_tag="VFY74_09335"
     CDS             complement(12110..13015)
                     /gene="rimK"
                     /locus_tag="VFY74_09335"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEX5931934.1"
                     /translation="MKIALLARNPHLYSHRRICEAARARGHDIEVINTLRVSINITSR
                     RPTLYYRKRQLDDFDAVIPRIGASVTFYGLAVLRQFEVMGVYPLNESVAIGRSRDKLR
                     CLQLLARRGIGLPVTAFTHDPNQAEDIIDLVGGPPVVIKLLEGTQGIGVVLGETVKSA
                     KSVIEAFQGVNINILVQQFIEEADATDIRALVIGEKVVAAMQRSGAPGDFRSNLHRGG
                     AGEKVRVTPQERRAAVGAARTLGLNVCGVDMLRSKNGPLVVEVNSSPGIEGLEQATGT
                     DVAGLMIDFLERNAAPGQTRTKGKG"
     gene            complement(13050..13559)
                     /locus_tag="VFY74_09340"
     CDS             complement(13050..13559)
                     /locus_tag="VFY74_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HEX5931935.1"
                     /translation="MEASLNPNDRPSIGWREWVALPELGIGAIKAKVDTGARTSALHA
                     YKIEPFRRAGALWVRFELHPMQRSDAMKVACEALAIDERTVRNSGGGLEHRYIIRTRL
                     QLGTTSWPIELALANRDQMGFRMLLGRTALEDRVLVEPGRSYLLGTRRTSPRQRRKLP
                     SLKVGKLLR"
     gene            13692..13913
                     /locus_tag="VFY74_09345"
     CDS             13692..13913
                     /locus_tag="VFY74_09345"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX5931936.1"
                     /translation="MGRNQHVVSRGEGWAVEAEGEAKPVAIFKTQSEAWEKAKSIARK
                     ERSEAFLHAKNGQVRARNTYGYDPRRSKG"
     gene            14050..14217
                     /locus_tag="VFY74_09350"
     CDS             14050..14217
                     /locus_tag="VFY74_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931937.1"
                     /translation="MDQQSRTIRDASIGAIAALSILSALSVAACAVKQPYEFPAMRLT
                     SPPGVDGESRQ"
     gene            14248..14415
                     /locus_tag="VFY74_09355"
     CDS             14248..14415
                     /locus_tag="VFY74_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931938.1"
                     /translation="MRFFIGALALLVIVAVLASPAALLLGWVQLADIREITAPSATDP
                     ALAPAAEPAKQ"
     gene            14472..15119
                     /locus_tag="VFY74_09360"
     CDS             14472..15119
                     /locus_tag="VFY74_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5931939.1"
                     /translation="MSPSDRWRRPHSYHHGNLREVLLEAARKLIEQYGPAGFSLTEAA
                     RLAGVSPAAPYRHFRDRDALLAEVARSGFERFAARLDTAWNNGIPTPLSAFEQLGRAY
                     LAFARDEPASYAVMFEAGFTEASREPIPAAERAFDVLQKAAAALCRQLPESERPPVKL
                     MSLHIWAISHGVATLFAQGEVRKVPMKAEEILESAMLIYLKGLGILSDTKAAPTR"
     gene            complement(15364..16095)
                     /locus_tag="VFY74_09365"
     CDS             complement(15364..16095)
                     /locus_tag="VFY74_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX5931940.1"
                     /translation="MTSFLVAAGAKDGAARSLRDARPHNGVVAAHTMPVQGIDVSYWQ
                     GDIDWRAVRAAGIHFVFIKATEGGDHIDPKFLDNWYGAKRAGVARGAYHFIYWCRPAH
                     EQALWFMLNVPADADALPPVLDLEWNTHSRTCPKRVRRDDALKTIRTLLAALQTHTGK
                     RPIIYTDPKFHREVLEGAFPDYHFWLRSVAAEPREVYRDRSWTFWQFTTTGTVPGVSG
                     KVDRNSFNGTRADWERVLNWLQAER"
BASE COUNT         2908 a         5199 c         5106 g         2885 t
ORIGIN      
        1 tcgaaggcga acttgaaata gctgcgccac gctctgtcgt cgttgaagag cgtcttggcc
       61 tgctcagccg gcatgtgatt gtagaagcca taatcctcgg cggcgaattt gaagaagcgg
      121 cgctgccaaa acgggcgggg gctctgccac cagccgtgca gccgccaatt ctgcgccccg
      181 cgcgccgaag cgcgcagcgc ctcatcgatc ttcgtgagag gagcaatgat atcgtccggc
      241 ccgcacagct ccgacagggc aagctcgatg ctcgttcccg ccgtcttctt ggtcttgaga
      301 aaaatgaact tgtgctcgtg cgagatgatc atcttggcgc gccgcgggct cgtcgaaact
      361 gtcgcaaacg tttgcggcac cttagtccag cggcacttcg cggcacaagg cgcgagggac
      421 ggctagagca ttgtcccatc gaattgaacc atgaccccct cttactcccc cctttcaggg
      481 gggagaaaaa ttcggagcgg cgtatcgctc tccctctcct gcaaggggga gggccggggt
      541 gggggtcggg agcgtggttc gatgcgattg aattatgctc taacgcggct ctttggcgag
      601 ccatatggcg agatcttcga ggatcgcgcc gaaatggatc tcggggattt gcagcgcggc
      661 ggcgatgtcc ttgagcttct tgcgctcctc gcctgcgatg cggttgtcgg caaggagcac
      721 ccggtaggcg cccttgatga cctcctcctt ggcgccccgg tcgagcgtct ttgccgccgc
      781 ggcgaactcg gcgatggcat cgcccttggc gagcgcgtcg gcggcgcgcg ccacctcgtc
      841 ggcgctgagc gcgtggcccg actgatcctg atagaccttt tggatgagac cggtctcgcg
      901 cgcgtcaagc cctccgtcgg aggccgcgac cgcgaccatc gccttgagaa cccaatcgtt
      961 gtcttgagtt gccagcttgt cccccatctc cgctccccac ggttccgcgc aaggcccgcg
     1021 attgtgcgcg gctgaggcca ctttgccaag cgacgatgtt gcccgcgcct cggtcccctg
     1081 tgttagtttg gctattcagc gcagcgatgc caaggaacga gccggtttgc gtaacacgag
     1141 acgggagtcc gggcctgacg ttccattcgg gacatttcgg ccgaatcttg cgcaaagaag
     1201 cttgatgcgg gtcgggcggc taatcccacc cggccgctac gcgcggaaga tggcgcggga
     1261 gatgcgcatc ctggtgcttc cgcatttgtt cgaccgctac gagctacgac tgctttctcg
     1321 cgtcatgcat ccgggctcgg tctttatcga tgtcggagcc aatgtcggaa tctataccgt
     1381 ctttgccgct ttgcgtggcg ggcccacgtc acgcgtcttt gccgtcgagc cgaaccctgc
     1441 cgctctggag cgcctgcact gcaatctttc gctgaatggt ctcaccaacg tcgtggtcga
     1501 accgatcgct ctcggcgaga aggcgggact ggtgagcttc aagcttgacc gtcgcaacat
     1561 cggcaacagc tctctcgtat ttgaccgaga cgctcctgcg ggcgacctga tcgaggtcca
     1621 gggtcaaacg ctcatggatc tctgcgcccg ttacggcatc gtcaggcttg acgccatcaa
     1681 gctcgatgtc gagggcgcgg aagaccagat tctctttccc ttctttgccg aggcgccggc
     1741 ctccctctgg ccgaccttgc tgctcgttga gaacagcgag ggccagtggc ggcgagattg
     1801 tgcttctatg ctcatcgaga agggttatcg gcggaagcgc attccctccc gcaacctggc
     1861 gttctggcgc cctgcgccgc gaccctagcg ccgtcacgcc agcccatgaa gccgctcaga
     1921 gtcgtcttcc tcgccgacac caggcccggg cattttcacg tggcgcaagg ggtgattgcg
     1981 gcgcttgagc ggcttcgccc cgtcgaggtg acccgcatcg aggtcaagcg caaatggatc
     2041 gtgccgacgc gctggctccg gcgccgcatc acggccaaaa ccttctttcc gccgcgcatg
     2101 ctgcgcatgg cctatcgcat cgacgcttac gccctgccca aggccgatct cgtcgtctcc
     2161 gccggcggcg agacccagat gccgaacatc tgcgttgcgc gctttctcga cattcccagc
     2221 atcttttgcg gctcgctcct gcgcggattg tcgcctgaaa atttcagcct catcatctct
     2281 tcctacgagc gcgatgcgac aagcccccgt cacttggttg tgctcaagcc ctcctcgatc
     2341 gatcccgagg cgctcggacg cccgaagatc gtgccgcgct atggcccggg caagcggcct
     2401 gagcttgccg gtctcctgat cggcggcaat gccggtccgt tccgctatcg gcgcgccgag
     2461 tgggagcgcc tgctcgcctt cgcgggcgat gtctcgcggg cctggggcac gcgatggctc
     2521 gtctccacct cgcgccggac ccccgacttt gtcgctgatc gcgtcgcgga actcgccgct
     2581 aatagcggca tcgtttcgcg tttcatcgat taccggaaag ccgggccggg gaccctgccg
     2641 gaggtctttt cgcaggccga ggttattgtc tgcaccgagg attcgagcac gatgatctcg
     2701 gaggccgtgt gtgcgcgtct gccggttgtc ggtgccgcgc cgagcgctca tcgcttcacc
     2761 gacgaggagc aagagtaccg agacttcctc gtccgcaacg attggtgccg ggtgctgccg
     2821 atcgccgagc tgacccccga taccttcgcc cgcgcgcttt ccgagatcga ggtgttgcaa
     2881 gagaacccgc tcgatgcgct cgcggcgaag ctgaaggagc gcctgcccga tctccttggc
     2941 gatcgcgatt gagtctcgcg cgccgattgg ctagaagccg ttcttccctt gccaccgcat
     3001 tcaggagctt aacgtggcca cgcctcaata cgtctatgtc atgaaccgcc tgtcaaagac
     3061 ctaccagggc ggcaagcagg tattgaagga cgtcacgctc gccttcctgc ccggcgccaa
     3121 gatcggcgtc gtcggcgtga acggttccgg caagtcgacc ctgctccgca tcatggcggg
     3181 ccaggagacc gagttcgtgg gcgaagcctg ggccgccgaa ggcgtgagcg taggctatct
     3241 gccgcaagag ccggtcctcg acgagaccaa ggacgtcgcc ggcaatgtca tggagggcgt
     3301 cgctgggaag aaggcgaagc tcgaccgcta caatgagctt gccatgaact attccgacga
     3361 aacgacggag gagatggcgc agcttcaaga cgagatcgac gcgcaagggc tgtgggacct
     3421 cgacgcgcag gtggagcagg ccatggacgc gctctgctgc ccgcccggcg atactgacgt
     3481 gaagactctg tcaggcggcg agaggcgccg cgtggcgctg tgccggctct tgcttgccaa
     3541 gcccgacatt cttctgctcg acgagccgac caaccatctc gacgccgaga gcgtcgcctg
     3601 gctcgagcat catttgcgcg actatccggg caccgtgatc atggtgaccc acgaccgcta
     3661 cttcctcgac aacatcaccg agtggacgct cgagctcgat cgcggggccg gcatccccta
     3721 caagggcagc tactcgtcct ggctcgagca gaagaagatg cggctcgacg tcgagggcaa
     3781 gcaggacgag gcgaggcagc aaacgcttgc ccgcgagctc gattggatca gggcgagccc
     3841 aaaagcgcgg caggccaagt ccaaggcgcg catccgcgcc tatgaggaca tgctcgccga
     3901 ggcggcgcgc gacaaaggcg gcaaggcgca gatcacgatt ccgcccgggc cccggctcgg
     3961 cgatgtcgtg atcgaggccg atcagttgaa caaaggcttc ggcgacatgc tcctgatcga
     4021 cgaactctcc ttcaagctgc cgcccggcgg cattgtcggg gtgatcggac cgaacggcgc
     4081 cggcaagacg acgctgttcc gcatgatcgt cggccaggac aagcccgatg acggcacgat
     4141 ccgcgtcggc gagacggtga agctcggcta tgtcgatcag tcccgcgatg cgctcgacgg
     4201 caggaagtcg gtgttcgagg agatctcggg cggcaacgac gtgatcaagc tcgggacccg
     4261 cgacgtgccg tcccgcgcct atgtcggctg gttcaatttc aaggggtccg accagcaaaa
     4321 gaaggtcggc caactctcgg gcggcgaacg caaccgcgtg catctcgcca agatgctgaa
     4381 gtcgggcgcc aatctgatcc ttctcgacga gccgaccaac gatctcgatg tcgaaacact
     4441 gtcctctctc gaagcggcgc tcgaggattt ccccggctgc gccgtcatca tcagccacga
     4501 ccgctttttc ctggatcgca tcgccaccca catcctgtcg ttcgagggcg acagccacgt
     4561 cgagtggttc gagggcaatt tcgaggacta cgaggaggac aagaagcgcc ggctgggcga
     4621 ggacgccgtc ctgccgaaac gcatcaaata caagcacttc gcccgccggt gaggcggggc
     4681 ccttgcatct cttcataaaa acgtataaac atatctttat gtccagtgat gagaaaagcg
     4741 gaccaccagg tttatcccgt gcccaagcct tggcaggctt gcgcgcggcc ggagaagcca
     4801 cgcggcttcg catcctggcg ctgctctccg ccggcgagct gaacgtcaag gacctgaccc
     4861 agatcctcgg ccagagccag ccgcgcatca gccggcacct gaaactcatg gccgaagcgg
     4921 ggctgatccg gcgcttccgc gagggctctt gggtcttctt ccgtctcgcc gattccgggg
     4981 cggaagccgc gctcgcccgc gccattgtcg cgagcctcga ccccaccgac cttactctcg
     5041 cgcgcgaccg cgcgcgggct tccgccgtcg agaagacacg cgccgaggcc gcgcaagcct
     5101 atttcaagac gcatgcgagc gaatgggatg agatccgcgc gcttcacgtg gccgagaagg
     5161 acgtcgaagc cgccatggat gcggcgctcg ggcgggaccc ctttgggctg ctcgtcgatc
     5221 tcggcaccgg aacgggccgc atcctcgagc tcttcgcttc ccgcgcgacc cgcgcgctcg
     5281 gcttcgatct caatcacgcg atgttggcgt acgcgcgcat gaagctcgag cgggctgggc
     5341 tctcccatgc gcaagtgcgc cacggcgatc tcaacaacgt cccgctaccg gacggcgccg
     5401 ccgacgccgt ggtgctgcat caggtcttgc attttctcga tgacccggcg gcggccattg
     5461 ccgaagcggg gcgtctcttg gcgccgggcg gaaggctgct tgtcgtcgat ttcgccccgc
     5521 atgagttgga gttcttgcgc gagcagtcgg cccaccgcag gctcggcttc ggacgcgccc
     5581 agcttggccg cctgttcgaa agcgcgggcc tcaagctcga gcgcttccgc gagctcaagc
     5641 ccgcgccgtc cggcggcaag ctgaccgtat cgctctggct cggcctgaag cccgccgctg
     5701 ccgcgaagaa gcggaaagtg ggcgccaagg tggaggccgc cgcgtgatgc tcgcatcctc
     5761 cgaacccaag agccgcattc tcggccgcgg cgccatcgac gtatcgttcg agttctttcc
     5821 gccgaagacc gaggcgatgg aacagactct gtggagctcg atccgccgcc tcgaaccgct
     5881 caggccgcgc tatgtctcgg tgacatatgg ggccggcggc tccacgcgcg agcgcaccca
     5941 tggcacggtc gcgcgtatcc tgcgcgagac cacgctgaaa cccgcggcgc acctgacctg
     6001 cgtcgaggct acgcgcgagg agatcgacga tatcatccgc gattattggg atcttggcgt
     6061 gcgccacatc gtggcgctgc gtggcgatcc tcccggaggc gtcggtcagc gttaccagcc
     6121 gacgcccggc ggctatggta acgccgccgg cctcgccgag ggcatcaagg cgattgcgcc
     6181 attcgaagtt tcggtcggct gctaccccga gaagcatccc gagtcgccga gcgtcgaagc
     6241 cgatatcgac gtgctcaagg ccaagatcga cgccggcgcc acgaccgcga tcacccagtt
     6301 cttcttcgat aatgccatgt acttccgcta tctcgatcgc gtccgcgcgc gcggcatcac
     6361 cattcccatc gtgccgggca tcgttcccat ccacaatttc aagcagatcg cgatcttcgc
     6421 cgcaaaatcg ggcgcgagcg ttccggactg gctcggccat cgattcgaag gcttggacga
     6481 cgaccaggag acgcgtcatc tcgtggctgc cgccgtcgcc gccgagcagg tccttggcct
     6541 ggtcgaccag ggaatcaaag agtttcattt ttacacgctg aaccgcgccg acctcgtcta
     6601 tgccatctgc catttgttgg gcctgcgccc ggcgaaggcg tttttggatg tgaaggcggc
     6661 gtcatgaccc ggaaacagcg tatcgaagcg ttgaagcggg aagcgaagca acgcatcctc
     6721 attctcgatg gcgccatggg caccatgatc cagcgccata agcttgacga agcaggctac
     6781 cgcggcgatc gctttaagga tcatgcgcgc gatctgaaag gtaacaacga cctcctggtt
     6841 ttgactcagc ccaaaatcat cgccgccatc cacgacgcct atctcgaagc cggcgccgat
     6901 atcgtcgaga ccaacacctt caacgcgcag gccatcagcc agtccgatta cgggctcgag
     6961 gacatctctt acgagatgaa tgtcgccgcc gccaaactcg cgcgcgcgtc ggccgacgct
     7021 tggaccaaaa agacgcccgg caagccgcgc ttcgtcgcgg gcgccatcgg cccgaccaac
     7081 cgcaccgctt cgatctcgcc ggatgtgaac aatcccggct tccgcaacgt gagcttcgat
     7141 gctctggtcg aggcctacgc cgtgcaagcg cgcgggctga tcgagggcgg ttgcgacttc
     7201 gtcctcatcg agacggtgtt cgacacgttg aatgccaagg ccgggggctt cgccgtcgag
     7261 caggtgttcg atgaactggg cgagcgtgtg ccggtcatgg tctcgggcac catcacggat
     7321 ctttcgggac gcaacctctc gggccagacg cccgaagcct tctggtactc gatggagcat
     7381 ctgcgcccgt tctccatcgg actgaattgc gcctttggcg ccgagcagct caggccttgc
     7441 gtcgacgaaa tcgcgcatgt ggccgcgacc tatgtcagcg tctatcccaa tgccgggctt
     7501 cccaacgaga tgggcgagta cgacgagacc cccgaattca tggcgggtct gctcgaggaa
     7561 tgggcgcggg acggcctcct caacattgtc ggcggctgtt gcggcaccac gcccgagcat
     7621 atccgcgcca tcgccgcggc ggtctccaag tacaagccgc ggcgcgtgcc ggaagtcgcg
     7681 cacaagctcc gcctctcggg cctcgatccc ttcgtgcacg ggtaaagcgc tgacatgagc
     7741 aacggcgccc agaacttcgt catggtcggc gagcggacca acgtcaccgg ctcggctcgt
     7801 ttccgcaagc tgatcgaggc gaacgattac gccgcggctc tcgaagtcgc gcgccagcag
     7861 gtcgagaacg gcgcgaacat gctcgacgtc aacatggacg agggcatgct caattccgag
     7921 caggctatga ccaccttcct cagcctgatc gcggccgagc cggacatcgc ccgcgtgccg
     7981 atcatgatcg attcctcgaa atggtcggtg atcgaggccg ggctgaaatg cgtacaaggg
     8041 aaggcggtgg tcaattccat cagcatgaag gaaggcgagg ggccctttct cgaccacgcg
     8101 cgcaaagtgc gccgctttgg cgccgcggtc gtggtcatgg ccttcgacga aaagggccag
     8161 gccgaatcgg ttgagcgcaa gttcgagatc tgcaagcggg cctaccgcct cctgaccgaa
     8221 caggtcggct tccccgcgga agacatcatc ttcgatccca acatcttcgc tgtggcgacc
     8281 ggcatcgagg agcacaacga ctatgcgcgc gcctatatcg aggcgacgcg gcggatcaag
     8341 gccgagctcc ctttcgtgca tgtgtcgggc ggcgtctcga atctctcgtt tgcctttcgc
     8401 ggcaacgaac gcgtgcgcga agccatgcac tcggtgttcc tctatcacgc gatcgaggcc
     8461 ggcatggata tgggcatcgt caatgccgga cagcttgcgc tctacgacga catcgagcct
     8521 gagctgcgca cactcgtgga ggacgtgatc ctcaaccgcc acgcggacgc gaccgaccgg
     8581 ctgctcgaag cggcccaacg tttcaagggg gagggcgccc agaaacgcga ggtcgatctc
     8641 tcctggcgcg agaagccggt cgacgagcgc ctgatccacg cgctcgtcca cggcatcggc
     8701 aactatatcg acgaagacac cgaggaagcg cgggcgcacg ccgagcgtcc gctccatgtc
     8761 atcgaggggc cgctgatggc gggcatgaac gtcgtcggcg acctgttcgg ctcaggcaag
     8821 atgttcctcc cgcaggtggt gaagtccgcc cgcgtgatga agcaggccgt cgcctatctc
     8881 atgcccttca tggagaagga aaaggaggag cttgggctcg cgcaaggaga acccgccggc
     8941 aagattcttc tcgccacggt gaagggcgac gtccacgaca tcggcaagaa catcgtcggc
     9001 gtcgtgctcc aatgcaacaa ttacgaggtc accgatctcg gtgtcatggt gcccgcgcaa
     9061 gtgatcctcg agacggcgcg cgagaagaaa gtggacatca tcggcctctc gggactgatc
     9121 acgccgtcgc tcgacgagat gtgccatgtc gccgccgaga tggaacgcga gggcttcgat
     9181 ctgccgctcc tgatcggcgg cgccaccacg agccgcgtgc ataccgcggt gaagatcagc
     9241 ccgaactacc gcaagagcca ggcgatctat gtgaccgacg cgagccgcgc cgtcggcgtg
     9301 gtgtcgagcc tgatgtcggc ggaggaacgg cccaaagcga ttgcaaaagt gcgcgaagag
     9361 tatcacagca tggcggactc ctatgcgcgc gggcaggccg acaagcgccg cgtctcgcta
     9421 tcggaggcgc gcgcgaacaa gctcaagctc gattggacgg gctacgtgcc gccgaagccg
     9481 tccttcatcg gcacgcgcgc cttcaagagc tacgacctcg ccgacctcgc ccgctatatc
     9541 gattggacgc cgttcttcca cgcctgggag ctgaccggga cgtttccccg tatcctcgac
     9601 gacgacaaat atggcgaggc cgcgcgcgat ctctttgaag acgcgcagag catgctgacg
     9661 cagctcatcg aggagaagtg gctgaccgca aacggtgtcg tcggcttctg gcccgcgaac
     9721 agcacgggcg acgatatcga gctttacacc gacgagacgc gcacgaagcg cctcgccacc
     9781 ctccacacca tccgccagca gatggaccgg gacaaggaca agcccaatct cgcgcttgcc
     9841 gatttcgtcg cgccgaaaca aacgagactt gccgactatg tcggcggttt cgccgtgacg
     9901 gccggtatcg gcgaggagga cacggcccgg cgcttcgagc gcgccaacga cgactattcc
     9961 aagatcatgg tcaaggcgct cgccgaccgc cttgccgaag ccttcgccga ggcgctgcat
    10021 gaaaaggtgc ggcgtgagtt atgggcgtat gcgcccgggg agaagctgac caacgaagag
    10081 ttggttgcag aggcctacgt cggcattcgc cccgcgccgg gctacccggc ccagcccgac
    10141 cacacggaga agggcacgct tttcgctgtg cttggtgcgg aaaaggcgac cgggctcaaa
    10201 ttgacggaat cttatgccat gtggccggcg gccgccgtct cggggctcta cttctcgcac
    10261 cctgagtcgc gctatttcgg cgtgggcaag atcggccgcg accaggtcga agactacgcc
    10321 ttgcgcaaag gctggagcct tgccgaggcc gagcgctggc tcgcgcccat cctcagctac
    10381 gacccgaagc tgatcgccga ggcggcggct tagcctgcgg ccggcacaaa tccgccccgg
    10441 ccccgggccg gtaacataaa gctgcgaaag ggtttgaggc ttttaagcta gatcatcccg
    10501 aggatgacgg cagcgataaa gccaaacgac attaccgcca tgagggcggt cagcttggct
    10561 gtcagatcca tcggaaactc ctttccgatt ggaggcgtgc ccttctagcg gttcaccaag
    10621 tttaggggcg gccccttgca tcgccaagac gtttccttgt tgcactgcac aactgcggtg
    10681 ccgtcaacgt cttggctcta aacgcaggcg cgggggattt ggttcatcgg ggctgcgatc
    10741 gcagaaatcc ggcgcgatta acggccttgc cggcccaatg aaaatcaatg cagtaggcgg
    10801 cacagtttag ccgcagcggc aacaaattcc gggcgctctt gtccgatagg ggagcgctgg
    10861 agcctgatct tgcgggggaa gagccctgcg ccggtatggg ttggccgttt catccagagg
    10921 gaatagatga agactgtcac cgcttcgggc ctgggcttgc tgggcctcct tattgccctt
    10981 ccggcgcagg ctctcaccat caccaacacc gactccgacc ctcgtaccgt cacggtcaaa
    11041 gccggcagcg ataacaaggc gctgaccgtc gaagccggga aggcggtaga gccgccctgc
    11101 gatccgagtt gcacgatcga gctcgagaac ggcgagctct atgagatgca aggggacgaa
    11161 gtggtcgcga tcgaggatgg catcatcttc gtcgatgccg tgacaccata agcagataga
    11221 cggaccgcac gacacgccgg gcagcttcgc ggcacgaccg ttcaccgcgt gatcgggacg
    11281 gggcctgttt gcgagatcat cgggcagtcg agtgtcttct ccagggcgtc gaccaaatcc
    11341 tgctctgagt tggcgcgggc gaagagcccg cgattctcgt tggcgagaca catgaggtcg
    11401 accacgctgt tgccccccgt ccaggagaag ccttcatagc ggaaggcgat ggcgtgaacc
    11461 gtcagctggt ggccaagaag ccgaagctgt ttgccgagtt cgcagggcaa cccgccgcac
    11521 gtctcctcgc cgtcggtcac gacgacgatc acgccaggct tgttgcgata gttgagcacc
    11581 tccgccgcct gctggacagc cgaggtgagc ggggtcttgc cggcgggaac caaggcgttc
    11641 accgcgcgca tgatctgctt gaaggcgtcg gccgtcggct tgaggtcgag cttgacgttg
    11701 cactggttgt aaggtccggg cccgtaggtg atgagaccga cgcgacgatg cttggtcgcg
    11761 gtcggcagga cccgcgcgag cgccgcacgc acctcatcga tccggggcct cgagttggga
    11821 atgccgagcg tctggttgcc cgacatcgaa cccgacgcat cgaacacgat catggcgtcc
    11881 tcggtgcaag gcgagggcgc cttctcctcg gcggcagccg gactgccggc caagatactg
    11941 gttagggcta tgactccgaa ccggaaaccg ccacgcaagg caatccggcc ccgggcaaac
    12001 attctcgcca ttgctcaact ctcccgaaag ccgtgccgac tttagcgtaa gaaacgggga
    12061 tgcaaagggg ggggtcgtgc cgcctccgga agcggaggct gacaaggcgc tagcccttgc
    12121 ccttggtgcg ggtttgcccc ggggctgcgt tgcgctcgag gaaatcgatc atcaggcccg
    12181 ccacgtcggt tccggtcgcc tgttcaagcc cctcgatgcc tggcgacgaa ttgacctcga
    12241 cgacgagcgg cccatttttc gagcggagca tgtcgacacc gcaaacgttg agcccgagcg
    12301 tgcgcgcggc tccgaccgcg gcacgccgtt cctgcggcgt gacgcgcacc ttctcgcccg
    12361 cgccgccgcg atgaagattg gagcggaagt cgccgggcgc gcccgagcgc tgcatcgccg
    12421 ccaccacctt ctcgccgatg acgagcgcgc ggatatcggt ggcgtctgcc tcctcgatga
    12481 attgctgcac aaggatgttg atgttcacgc cctggaacgc ctcgatcacc gacttcgccg
    12541 acttcaccgt ttcgccgagg acgacgccga tgccttgcgt cccctctaag agcttgatga
    12601 ccacgggcgg accgccgacg agatcgatga tgtcctcggc ctggttcggg tcgtgggtga
    12661 atgcggtcac cggaagccca atcccgcgcc gggccaagag ttggaggcag cgaagcttgt
    12721 cgcgcgagcg gccgatcgcc accgactcgt tcaacggata aacgcccatg acctcgaatt
    12781 gccgcagcac ggccagccca tagaacgtca ccgaggcgcc gatgcgcggg atcacggcat
    12841 cgaaatcgtc aagttgccgt ttgcgatagt agagggtggg gcggcgcgag gtgatattga
    12901 tggaaactct gagcgtgttg atgacttcga tgtcgtgtcc ccgcgcccgc gccgcttcgc
    12961 aaatgcggcg atggctgtag aggtgtggat tgcgcgcgag caacgctatc ttcattggag
    13021 actcgtgacg aaggggtggg aatgatccgc tatcgcagca atttccccac cttcaaggat
    13081 gggagcttgc gcctttggcg tgggctcgtc cgtcttgtgc ccaagagata agagcggccg
    13141 ggctcgacca gaacgcgatc ctccaacgcc gtgcggccga gcagcatgcg gaagcccatc
    13201 tgatcgcgat tggcaagagc gagctcgatc ggccaagacg tggtcccgag ctggaggcgg
    13261 gtcctgatga tgtagcgatg ttccaggccg ccgcccgagt tccgcaccgt acgctcatcg
    13321 atcgccagcg cctcgcatgc gaccttcatg gcatcgctcc gttgcatcgg atggagctcg
    13381 aaccggaccc acagcgcgcc ggcgcggcgg aagggctcga ttttgtaggc gtggagcgcg
    13441 ctcgtcctcg ccccggtatc gaccttcgcc ttgatcgcac cgatgccgag ttccggcagc
    13501 gcgacccatt cgcgccagcc gatcgagggc ctgtcgttgg ggttgaggct tgcttccatg
    13561 gctcggcgat ggatcgcgcg gaattttgct ctgaccgatt tagcacagcg ccaccgaatt
    13621 ggctcggctt caaggggagg tcgccctcga ctatagtgcc ctggcgaacg gctcttggga
    13681 gaatgggggc catgggcagg aaccagcatg tggtcagccg cggcgagggc tgggcggtcg
    13741 aggccgaggg ggaggcaaag ccggtcgcca tcttcaagac ccaaagcgag gcctgggaga
    13801 aggcgaagtc gattgcgcgg aaggagcgct ccgaggcgtt cctgcatgcc aagaacgggc
    13861 aggtccgcgc gcgcaacacc tatgggtacg atccgcgccg cagcaaaggc tgaccggacc
    13921 cccgcttttc ttcgctgcgc caacgccgca tcagcgattc atttactgag tttgcgaatt
    13981 tcggcggccg tttagcgctc tttgtttgca tctcccttgc gaagctcctc cgcaaaaagg
    14041 aggcgctcca tggaccagca atcacggacc attcgagacg cgtcgatcgg cgcgatcgcc
    14101 gcgctctcga tcctgtcagc actgtccgtc gccgcctgcg cggtcaagca gccttacgag
    14161 tttccggcca tgaggctgac cagtccgccc ggggtcgacg gggaatcccg gcaataggcc
    14221 gagggacaag cccggaggga gagttcgatg cgtttcttca ttggtgcact cgcacttctt
    14281 gtcatcgtcg cggtgttggc ctcgcccgcc gcactgctgc tcggctgggt gcaacttgca
    14341 gacatccgcg agatcacagc ccccagcgcc acggatcccg cgctggcgcc tgcggcggaa
    14401 ccggcaaagc aatagacagg caatttgcct ttgctggcgg ccgcaagggc tttcatgtaa
    14461 aagcccttta catgagtccc tccgaccgct ggcggcgccc gcattcctat caccatggca
    14521 acctcaggga ggtgctgctc gaggcggcgc gcaagctgat cgagcaatac ggacctgcgg
    14581 gtttcagcct gaccgaggcg gcgcggctcg cgggcgtcag ccccgccgcg ccctatcgcc
    14641 atttccgcga tcgcgacgcg cttctggccg aggtggcacg ctcagggttc gagcgcttcg
    14701 ccgcgcgcct cgacacggcc tggaacaacg gcattcccac gccgctctcg gccttcgagc
    14761 aactgggccg cgcctatctc gccttcgccc gcgacgagcc tgcgagttat gcggtcatgt
    14821 tcgaggccgg gttcaccgag gcgtcgcgcg aaccgatccc ggccgccgag cgcgccttcg
    14881 atgtgttgca gaaggcggcg gccgctctct gccggcaatt gcctgaaagc gagcgtccgc
    14941 cggtcaagct gatgagcctg catatctggg cgatctcgca tggcgtcgcc acgctcttcg
    15001 cccaaggcga ggttcgcaag gtaccgatga aggccgagga gatcctcgaa agcgccatgc
    15061 tgatttacct gaaaggtctc ggcatcctct cggacacgaa ggctgcaccg acgcgatgag
    15121 tcctttttta acgtccccat cgtggggatg gaggagtccg agcatgcgcc gctattctaa
    15181 cctccccctc gtggggaggt ccgacgccgc tgaaagcggc gtcggggtgg ggggcgtgcg
    15241 tctcacgtgg tgaaaccgtc gcgcattgaa acgccatcac cccacccggc tcggccttcg
    15301 gcctcgccgc cctccccatc gagggaaggg ttaagcccga gcaaggggag ggggatgtga
    15361 cgatcagcgc tcggcctgga gccagttcag cacccgctcc caatcggcgc gcgtgccgtt
    15421 gaagctgttg cgatcgacct tgccgctcac gcccggcact gtcccggtgg tggtgaactg
    15481 ccagaacgtc caagaccgat cgcgatagac ttcgcgaggc tcggcggcga ccgagcgcag
    15541 ccagaaatga taatcgggga atgcgccctc gagcacctcg cggtggaact tgggatcggt
    15601 gtagatgatc ggccgcttgc ccgtatgggt ctgcaacgcc gcgagcagag tgcggatcgt
    15661 tttcagcgcg tcgtcgcgcc gaactctctt cgggcaggtc ctggaatggg tgttccattc
    15721 caaatcaagc accggcggca gcgcgtccgc gtcggccggc acgttcagca tgaaccacaa
    15781 cgcttgctca tgcgccggcc ggcaccagta gatgaaatga taggcgccgc gcgccacgcc
    15841 tgcgcgcttt gccccatacc aattgtcgag gaacttcgga tcgatgtgat cgccgccctc
    15901 tgtcgccttg atgaagacga agtggatgcc ggccgcccgc acggctctcc agtcgatatc
    15961 gccttgccag taggagacgt cgatgccctg gaccggcatg gtgtgggcgg cgacgactcc
    16021 attgtgcggc ctggcgtcgc gcagggaccg ggccgcgccg tctttggcgc cggcggcaac
    16081 aaggaacgac gtcacggc
//
LOCUS       DASUQP010000209         5842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_785369,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000209 DASUQP010000000
VERSION     DASUQP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785369"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFY74_09370"
     CDS             <1..981
                     /locus_tag="VFY74_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5931941.1"
                     /translation="GGEPLVVYFDDIRLVRDLLGDYDANLAVLEDRLGVEAVVNGNAV
                     SLRGSEISCATAKSVLEQLYGRLAQGEAIGVGEVVGAIRHARALEPSDDGAPGSSLPE
                     ARQIRTRKRLITARTPVQSAYLASLERNDLVFATGPAGTGKTYLAVAFAAACLESGRC
                     DRLILSRPAVEAGERLGFLPGDMREKVDPYLRPLYDALYDVLAPERVERGLASGAIEI
                     APLAFMRGRTLSHAIIILDEAQNCTAVQLKMALTRIGDESKMIVTGDPTQVDLPPGKD
                     SGLLEAIKLLKGIDDIDHIAFTRADVVRRELVGKIVDAYESAPLKGGRHG"
     gene            974..1513
                     /gene="ybeY"
                     /locus_tag="VFY74_09375"
     CDS             974..1513
                     /gene="ybeY"
                     /locus_tag="VFY74_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5931942.1"
                     /translation="MADGGPSRRRPVIGGVSLAIETVRREPAWARAKVTDALLKQAAR
                     AALRAAPPSGPSHSITLVLTGDAEMRDLNRLWRGKEAPTNVLSFPADAKRDRGFLGDI
                     VLAYETTLREANERDISLADHVSHLVVHGMLHLLGFDHADDEAAQRMEEIERIALSAI
                     GVADPYAETDEPRHAEASP"
     gene            1510..2481
                     /locus_tag="VFY74_09380"
     CDS             1510..2481
                     /locus_tag="VFY74_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5931943.1"
                     /translation="MSNTPAAEKDLPQQETPQTEEEVSWFDRLLQTFGLGEEPDLREL
                     IEDALARSKSDTLSGQERSMLRRILRFGTLRVEDVMVPRADVVAVDDTVTVAELMHVF
                     REAELSRVPVYHETLDDPRGMIHIRDLMSWITAEAETGDGAGLDLGKVDLTRTVGSIK
                     ITRESLYVPASMSVLDLLLKMQTTRLHLALVVDEYGGTDGLVSIEDLIEEVVGEIADE
                     HDVEVEPLIRSDPRLGLVADARMPIEEFEKHLGLALVSDDDGEDIDTLGGLAFSIAGR
                     IPARGERVRHRSGVEFEVLEADRRRIKKLRVHLPPAPDATDPESQQP"
     gene            2537..4201
                     /gene="lnt"
                     /locus_tag="VFY74_09385"
     CDS             2537..4201
                     /gene="lnt"
                     /locus_tag="VFY74_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX5931944.1"
                     /translation="MLSKANGIVTRAATVVAALRGARRAIAAVVLGALSVLAFAPFHA
                     WPVLFFTFGALVWLLDGCHAEHARLAQRVKAAAFVGFCFGFGYFLAGFYWIAEAFLVE
                     PWRHGWLLPFVMTALPGGMALFFAASAALAMLLWLPGAGRAFALAIAFGLAEFARGHV
                     LTGLPWNLVGYSLLGGLPLMQLASLFGIYALSLLAVLLFAAPAAIFAPSQSRLAGGAG
                     TVALAGLLLLLVALGYGWGERRLSLAGMDSAGLRLRIVQANVDQAEKWRPENSAEIFA
                     DYLELTRTGGASGLDGVAIVVWPETAVPFLLADDPDALAAIAAVLPEGTTLLVGSARL
                     VEERDAQGMLVRNRIYNSVLVIDDDGRVIGSYDKIHLVPLGEYLPFQDFLESLGMMQL
                     TGVRGGFSVGQGPRLLTIPDAPPATPLICYEIIFPHAITEAGTRPGWLLNITNDAWFG
                     SSAGPYQHFHQAQVRAVEQGLPVARAANTGISAVIDSFGRVRAEIGLGEKGIIDTDLP
                     KVGPPPLFARLDIIIDIAVLAFAAMGWLACRTYRMRAATFEAGCHN"
     gene            4374..4778
                     /locus_tag="VFY74_09390"
     CDS             4374..4778
                     /locus_tag="VFY74_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5931945.1"
                     /translation="MAGKKPNPVDAHVGSRVRLRRMLLGMSQERLGDSMGLTFQQVQK
                     YEKGVNRIGASRLFQISKILDVPVQFFFEEAPQLGERGHLRGMAEPESETFILEFLNS
                     REGLELNRAFVKIGDAKVRKTIVDLVRALSAN"
     gene            4886..>5842
                     /gene="metK"
                     /locus_tag="VFY74_09395"
     CDS             4886..>5842
                     /gene="metK"
                     /locus_tag="VFY74_09395"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5931946.1"
                     /translation="MSRANYVFTSESVSEGHPDKVCDRISDAVVDLYLAADPLSRCAI
                     ETLVTTNRIVMAGEVRGPDSVTPELIEQAARRAVREIGYEQAGFHWDLADFQFYVHEQ
                     SADIAAGVDPNAEKQKDEGAGDQGIMFGFACRETEPLMPAPIFYAHRILHEMAKARHS
                     GRAPELGPDAKSQVTLIYEDGRPVRADSVVVSTQHGEDVSQARVTEIVKNFVHEVMPK
                     GWVNGSTKFYVNPTGRFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVD
                     RSAAYAARYLAKNVVAAGLADKCTLELSYAIGVSQPLSLYVDL"
BASE COUNT          982 a         1917 c         1872 g         1071 t
ORIGIN      
        1 ggcggcgaac cgctcgtcgt ctatttcgac gatatccgcc tcgtccgcga ccttctcggc
       61 gactacgacg caaatctcgc cgtgctcgag gaccggctcg gggtcgaggc cgtggtcaac
      121 ggcaacgcgg tgagcttgcg cggcagtgaa atttcctgcg ccacagccaa atcggtgctg
      181 gaacagctct acggcaggct cgcccagggc gaggcgatcg gcgtcggcga agtggtcggc
      241 gcgatccgcc acgcgcgggc cttggagccg agcgatgacg gcgcgccggg ctccagcctc
      301 cccgaggctc gccagatccg cacgcgcaag cgcctcatca cggcgcggac gccggtgcag
      361 agcgcctatc ttgcctcgct cgagcgcaac gatctcgttt tcgccacagg cccggcaggc
      421 accggcaaga cctatctcgc cgtcgctttt gccgccgcat gtctcgagag cgggcgatgc
      481 gaccggctga tcttgtcgcg gccggcggtc gaggccggag agcggctcgg ctttcttccc
      541 ggcgacatgc gcgaaaaggt cgatccttac ctgcgcccgc tctacgacgc gctctatgac
      601 gtgctcgcgc cggagcgggt cgagcgcggg ctcgcatccg gcgccatcga gatcgcaccc
      661 cttgccttca tgcgcggccg caccctctcc cacgccatca tcatcctcga cgaggcgcaa
      721 aactgcacgg ccgtccagct caagatggcg ctgaccagga tcggcgacga gagcaagatg
      781 atcgtgacag gcgatccgac gcaggtcgac cttccgcccg gcaaggactc aggcctgctc
      841 gaggcgatca agcttctcaa gggcatcgac gacatcgacc atatcgcgtt cacgcgtgcc
      901 gacgttgtcc gccgggaact ggtgggcaag atcgtcgacg cctacgaatc cgctcccctc
      961 aagggtgggc gtcatggctg acggcggccc tagtcgcagg cgtccggtca tcggaggcgt
     1021 ctcgctcgcg atcgaaacag tgcgtcgcga gccggcgtgg gcgcgcgcga aagtgaccga
     1081 cgctctcttg aagcaggctg cacgcgcggc tttgcgcgcc gctccccctt ccggcccatc
     1141 ccacagcatc actctggtac tgaccggcga tgccgagatg cgcgacctca accgcctctg
     1201 gcggggaaaa gaggctccca ccaatgtgct gtcctttccc gccgatgcga agagagaccg
     1261 gggctttctc ggcgatatcg tgcttgccta cgagacgacg ctgagagagg cgaacgagcg
     1321 cgatatctct cttgcggacc atgtctctca cctcgtcgtc cacggcatgc ttcatctgct
     1381 cggcttcgat catgccgacg acgaggcggc tcagcgcatg gaggagatcg agcggatcgc
     1441 gctctccgcg atcggcgtcg cggaccctta tgccgagacg gatgagcccc gccacgcgga
     1501 ggcgtcgcca tgagcaacac gccagcggcc gagaaggacc tgccgcaaca agagacgcct
     1561 cagacggaag aagaggtctc ctggttcgac cggctgctgc agaccttcgg cctcggcgaa
     1621 gagcccgatt tgcgcgagct gatcgaggac gcccttgccc gcagcaagag cgatacgttg
     1681 agcgggcagg agcgcagcat gctgcgccgc atcctgcgat tcgggacgct cagggtcgag
     1741 gacgtcatgg tgccgcgcgc cgacgtcgtt gcggtcgacg ataccgtcac cgtcgcggag
     1801 ctgatgcatg tattccgcga ggctgagctt tcgcgcgtgc ctgtctatca tgagacgctc
     1861 gacgacccgc gcggcatgat ccatatccgc gatctgatgt cgtggatcac ggctgaggcc
     1921 gaaaccggcg acggcgcagg actcgatctc ggcaaggtcg acctcacgcg cacggtcggc
     1981 agcatcaaaa tcacgcgcga gtcgctctac gtgccggcat cgatgtccgt gctcgatctc
     2041 cttctcaaga tgcagacgac gcggctccat ctcgctcttg tggttgacga gtatggcggg
     2101 accgatgggc tcgtctccat cgaggatctc atcgaggaag tggttggcga gatcgccgac
     2161 gagcacgatg tcgaggttga gcctttgatc aggtcggatc caaggctcgg tctcgtcgcc
     2221 gatgcgcgga tgccgatcga agagttcgag aagcatctcg gtttggcgct ggtatccgac
     2281 gatgacgggg aggacatcga cacgctgggc ggtcttgcct tttccatcgc cggccggatc
     2341 ccggccagag gcgagcgcgt ccgccatagg agcggggtcg agttcgaagt gcttgaagcc
     2401 gatcggcggc ggatcaagaa gcttcgcgtc catctgcccc ccgctccaga cgccacggac
     2461 ccagagagcc agcaaccctg atgatgccgt tgggcgtgct ctcgcctacc ccctccagcc
     2521 aaggaggggg cggcccgtgc tgagcaaagc gaacggcatc gtgacgcgcg ccgccaccgt
     2581 cgttgcggcc cttcgcggcg cacggcgcgc gatcgccgcc gtcgttcttg gcgccctttc
     2641 cgtccttgcc tttgcgccgt ttcacgcctg gccggtcctg ttcttcacct ttggcgcgct
     2701 cgtttggctc ctcgacggct gccacgccga gcatgcgcgt ctcgcgcaaa gggtgaaagc
     2761 ggccgccttt gtcgggtttt gcttcggctt cggctacttc ctcgccggat tctattggat
     2821 cgcggaggcg ttcctggtcg agccctggcg ccacggctgg ctgctcccct tcgtcatgac
     2881 cgcgctcccc ggcggcatgg cgctcttctt cgccgcttct gccgcccttg ccatgctgct
     2941 ctggctgccg ggagccggca gggcgttcgc gttggcaatc gccttcggtc ttgccgaatt
     3001 cgcccgcggc catgtgttga ccggccttcc ctggaatctc gtcggttaca gccttctcgg
     3061 cggcttgccg ctgatgcagc tcgcctccct gttcggcatc tacgcgctca gtctccttgc
     3121 cgtgctcctc ttcgccgcgc ccgccgccat tttcgcgccg tcacagtcgc ggctcgcggg
     3181 aggcgcgggc acggtcgcgc ttgccggact tctcctgctt ctcgtggcgc ttggctatgg
     3241 gtggggcgag cgcaggctaa gcctggccgg aatggacagc gcggggcttc gcttgcgcat
     3301 cgtccaggcc aatgtcgacc aggccgaaaa atggcgccct gagaacagcg ccgagatctt
     3361 cgccgactac ctcgaactca ccaggaccgg cggtgcgtcc ggtctcgacg gggtcgcgat
     3421 cgtggtctgg cccgagacgg cagtgccctt ccttctcgcc gacgatcccg acgcgctcgc
     3481 cgccatcgcc gccgtgctcc ccgaaggaac cacattgctg gtggggtcgg cgcggctcgt
     3541 cgaggaacgg gacgcgcaag gcatgctcgt ccgcaaccgc atctacaata gtgttctcgt
     3601 catcgacgac gatggccgcg tgatcggcag ctacgacaag attcatctcg tcccgctggg
     3661 agagtatctc cccttccagg attttctcga aagtctcggc atgatgcagc tcaccggcgt
     3721 gcgcggcggc ttcagcgtgg ggcaagggcc gcgactgctg accatccccg atgcgccacc
     3781 ggcaacaccg ctcatttgct acgagatcat ttttcctcac gcgatcaccg aagcggggac
     3841 gcggccgggc tggctgctca atatcaccaa cgatgcctgg ttcggatcga gcgcgggtcc
     3901 ctatcagcat tttcatcagg cgcaagttcg cgccgtcgag cagggcttgc cggtcgcgcg
     3961 cgcggccaat accggaatct ccgcggtgat cgattccttt gggcgcgtgc gcgccgagat
     4021 cggtctcggc gaaaagggta ttatcgacac agacttgccc aaagtgggcc cgccgccgct
     4081 ctttgctcgg cttgatatca ttatcgatat tgccgtcttg gcctttgcag caatgggttg
     4141 gctcgcctgc cgcacctacc gaatgagggc tgccacattt gaggccggct gtcataattg
     4201 aacggtcagt caaattccgc gccgaatttc tcgcgccaag aacgcgcagc gccgaagcat
     4261 gcttgacggg ctacaaccgc gcgcctatga cggacgcgcg gcggggccgt ctcaccaagg
     4321 ttgtgagata gctgggctct ttttatcaaa caaccattgc gacgggtgag cgaatggcag
     4381 ggaagaagcc aaacccggtt gacgcgcatg tcggaagccg ggtgcgattg cgccgaatgt
     4441 tgcttggcat gagtcaggag cgtctcggtg acagtatggg gttgacgttt cagcaggtgc
     4501 aaaaatacga gaagggtgtg aatcgcatcg gtgcaagccg gctgttccag atctcgaaga
     4561 tcctcgacgt ccccgtgcaa ttctttttcg aggaagcgcc gcagctcggt gagcgcggtc
     4621 atctgcgggg gatggccgag ccggaatcgg aaaccttcat cctcgaattc ctgaacagcc
     4681 gcgaagggct cgaactgaac cgtgccttcg tcaagatcgg cgatgccaag gtccgcaaga
     4741 cgatcgtcga cctggtgcgg gccttgagcg cgaactgagc acttcttaag tcgagcggtt
     4801 gcgtgccggc ttgacctccg gctcagtggt tgtaagaacc gggcgcgcag gccgcccggc
     4861 cttacctctc ggacaggagt gcaaattgtc gcgggctaat tacgtcttca ccagcgagtc
     4921 ggtgtcggaa ggccatcccg acaaagtgtg cgaccggatt tccgacgcag tggtcgacct
     4981 ctatctcgcc gccgatccgc tgtcgcgatg cgcgatcgag acgctcgtca cgaccaaccg
     5041 catcgtcatg gcgggcgagg tgcgcggccc cgacagcgtg acgccggagc tgatcgagca
     5101 ggcggcacgc cgcgccgtgc gcgagatcgg atacgagcag gcggggttcc actgggacct
     5161 cgcagacttc cagttttatg tgcatgagca atccgccgac atcgcggccg gtgtcgaccc
     5221 caatgccgag aagcagaagg acgaaggcgc cggcgatcag ggcatcatgt tcggcttcgc
     5281 ctgccgcgag accgagccgc tcatgcccgc gccgattttc tacgcccacc gcatcttgca
     5341 tgagatggca aaggcccggc acagcgggcg cgcgcccgag ctcggccccg acgccaagag
     5401 ccaggtgacg ctcatctacg aggacggcag gccggtgcgc gcggactcgg tcgtggtctc
     5461 gacccagcat ggcgaggacg tgagccaggc gcgggtgacg gagatcgtca agaacttcgt
     5521 ccacgaagtc atgcccaaag gctgggtcaa cggctcgacc aaattctacg tcaatcccac
     5581 cggccgcttc gtgatcggcg gccccgacgg cgatgccgga ctgaccgggc ggaagatcat
     5641 tgtcgatacg tatggcggcg cggcgccgca tggtggcggc gccttttccg gcaaggaccc
     5701 gacaaaggtc gatcgctccg cggcttacgc cgcgcgctat ctcgccaaga acgtggtcgc
     5761 ggccgggctc gccgacaaat gcacgcttga gctgtcctac gcgatcggcg tctcgcagcc
     5821 gctctcgctc tatgtcgatc tg
//
LOCUS       DASUQP010000210         7643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1603667, whole genome shotgun sequence.
ACCESSION   DASUQP010000210 DASUQP010000000
VERSION     DASUQP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603667"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..1223
                     /locus_tag="VFY74_09400"
     CDS             537..1223
                     /locus_tag="VFY74_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5931947.1"
                     /translation="MFDDFSKRSKAVKAALDLAKERDWGDITLTDIARAAGLSLADLR
                     REFSCKTDILRAFQAELDAEVLSKPRAVGEPSNPRDRVFDIVMARFEAMQPYKPGLKR
                     IASYLCCRPGESAMLACSSLASQYWMLEAAGAKLDGPGGALRVGGLAAIYGRVFCVWL
                     EDDTPTLDKTMAALDRRLSRGERMLSGMESACADLCRFACGLMPRGWKRHEAKRDAPP
                     STGPAPAPSP"
     gene            complement(1827..2153)
                     /locus_tag="VFY74_09405"
     CDS             complement(1827..2153)
                     /locus_tag="VFY74_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931948.1"
                     /translation="MRRMLVGLAAILSVSVAGPALAVDMTEEIQEEEQPGSEVEIDMG
                     GGGAEIERKSGKPILDIGGEGGGEELIAPNDPDTPEASDPIDDELPGEGPQDLSGPDD
                     DDNLPD"
     gene            complement(2180..3469)
                     /locus_tag="VFY74_09410"
     CDS             complement(2180..3469)
                     /locus_tag="VFY74_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5931949.1"
                     /translation="MPKGLYARALIIIIAPMVLLQSVLTFVFLERHWQTVTRRLSTVT
                     VQNLGLLIDLYQANPQGADTLERLAEQNLGVRVRFVRGEELPQVRAKPFFDLLDSTLS
                     DELTRQIGRPFWIDTVGRSNFVDIRIKLDNAVMHAIARRSQTYASNSQIFLLWMVGTS
                     LVLLTVAILFLHNQIKPIVRLSEAAESFGKGRPPPADFRPRGAREVRQAAHAFIEMRD
                     RIERYVEQRTIMLAGVSHDLRTVLTRFKLQLALFEETPELEAMHADVDEMQAMLEDYM
                     AFAKGDSGEEIVRADIGELLGEVKSQAHTTKHIAVEVKEAPLVVPVRRHAFKRAIANL
                     VNNAARFAEHVNVSAAKQNGTLTVAVEDDGPGIPEDEREQVFRPFYRIDHARNQDSGS
                     TGLGLAIARDIARIHGGDIALSRSPLGGLKAVLKVPV"
     gene            complement(3553..4269)
                     /locus_tag="VFY74_09415"
     CDS             complement(3553..4269)
                     /locus_tag="VFY74_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5931950.1"
                     /translation="MTAPTPIKAKSGSPDDNAPHILVVDDDSRIRDLLARYLQDHGFR
                     VTTAIDVASARATMRSLSFDLLILDVMMPQESGIDFARSLRSESRLPILMLTARAEPE
                     DRIEGLETGVDDYLAKPFEPRELLLRVSNVLRRGGAAPTTGEVRMGDFIFHIARGELK
                     RNGEPIRLTERERELLRYFAQRPGMPVARHELAKDADSGGERAVDVQINRLRRKIEHD
                     PANPVYLQTVRGKGYILYPE"
     gene            complement(4266..4865)
                     /locus_tag="VFY74_09420"
     CDS             complement(4266..4865)
                     /locus_tag="VFY74_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5931951.1"
                     /translation="MTDLTETLAGDKAPRAKTVRGKPRGNGRARKRTSLPEAKLLEFA
                     ELLFFAYRDFTRDPDVILKDFGFGRAHHRVLHFVNRHSGLRVADLLDILTITKQSLSR
                     VLKQLIGKGYIVQRAGRSDRRERLLFPTDKGRVLAERLAAPQSVRLAEALKAAGPGAE
                     AHLRRFLEAMVNAEERPKVSSIMAASPVPLKESAQGGGS"
     gene            5047..5943
                     /locus_tag="VFY74_09425"
     CDS             5047..5943
                     /locus_tag="VFY74_09425"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX5931952.1"
                     /translation="MVTAPYDQREGFIWLNGKLVPWGDARLHVLTHGLHYASAVFEGM
                     RAYEGEIFKLTEHDRRLHDSAKILDFEIPYTVAELDKAAREVVRANKLADAYVRPIAW
                     RGPEQMGVSAQLTKTNVAIAAWDWGSYFDPAARAKGLRLTFAEFRRPDPRTAPVKSKA
                     TGLYMICTIEKHRAERLGYNDALMLDWRGQIAEATGANIFFVKDGALHTPLPDCFLDG
                     ITRQTVIGLAKKRGLKVIERAIMPEELTTFSECFITGTAAEVTPVSEIGQYKFAIGDI
                     TRILMDDFTAMVHPAKAVAVAG"
     gene            5984..6175
                     /locus_tag="VFY74_09430"
     CDS             5984..6175
                     /locus_tag="VFY74_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931953.1"
                     /translation="MGRPPLKQAYTVSYAKAGIELKRYTITALNQVDAETEADNRFNR
                     AHPELKVFEDSVSRRVEAH"
     gene            6244..6912
                     /gene="bluB"
                     /locus_tag="VFY74_09435"
     CDS             6244..6912
                     /gene="bluB"
                     /locus_tag="VFY74_09435"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HEX5931954.1"
                     /translation="MAEAGLAEKREGTPEFGPAFREQLHELLKWRRDVRRFKRKPLPP
                     GRIERLIAIACLSPSVGLSEPWRFVIVDDESRRAAIRACFEACNKDALSIQTPDRAAL
                     YARLKLAGLDDAPCQIAVFADRATAKGTGLGRLTMPEALDYSAVIAIHTLWLAARAEG
                     IGVGWVSILDPKRVAAILDVPEHWVFIGHLCVGYPHASDHRPALERAGWEHRHPPATA
                     MLYR"
     gene            7208..7474
                     /locus_tag="VFY74_09440"
     CDS             7208..7474
                     /locus_tag="VFY74_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931955.1"
                     /translation="MRKALLILGSAGLVVAACLLTLPGGEAQARVWKECQRIAFCSGC
                     RPYFRCRSCEYRRTCSGGLCGWGDVCVWGPYVRVLPRGARIIRR"
BASE COUNT         1266 a         2484 c         2527 g         1366 t
ORIGIN      
        1 ctgttccatt tttgcgctac cgccctgcta cttgagcgca aggcgcaccg ggtggggggc
       61 ttgcccttca agcggcaacc tcgtgattcc ggcgcacgcc ggaatccaga atcgaaagct
      121 gagccgggtt ggatgttggt ctttgcaacc tcgaccccgt cgcccacttt agcacccccc
      181 accccgacgc cgccctcggc gtcgtcggac ctccccacga gggggaggtt tcacgctgga
      241 ggggcgtcca gtggttcggc ggcttccttc caaaaagggg ggaaccttca cttccctccc
      301 cgcctctttt taacctcccc ctcgtgggag gtcggcgcgc cgctccattt ttgcgctacc
      361 gccctgctac ttgagcgcaa ggcgcaccgg gtggggagcg cttaggcatc ctctcacgtg
      421 aaagcagcac agatgcttgc gaagaggttc gcggggggct tgcgctcaaa tggcgctggc
      481 ggcgcctttc gacggccatc gaaagcgcct atgtttggtg cgaaggagta actcccatgt
      541 tcgacgattt ctccaaacgt tccaaggccg tgaaggcggc gctcgatctg gccaaggagc
      601 gagattgggg cgatatcacg ctcaccgata tcgcgcgcgc cgccggtctc agcctcgccg
      661 atctcaggcg cgagttcagc tgcaagacgg acatcctgcg cgccttccag gccgagctcg
      721 atgccgaggt gctgtccaag ccgcgcgcgg taggcgagcc gtcaaatccg cgcgaccgcg
      781 tgttcgacat cgtcatggcg cggttcgagg cgatgcagcc ctataagccc gggctgaagc
      841 ggatcgcgtc ctatctctgt tgccggccag gcgagtccgc gatgttggcg tgctcttcgc
      901 tcgcctcgca atattggatg ctcgaagcgg ccggcgccaa gctcgacggc ccgggcggcg
      961 cgctgcgcgt cggcggcctt gccgccatct acggcagggt gttctgcgtg tggctcgagg
     1021 acgacacgcc gacgctcgac aagaccatgg ccgcgctcga ccgaaggctc agccggggcg
     1081 aacgcatgct ctccggcatg gagtcggcct gcgccgatct ctgccgtttt gcctgcgggc
     1141 tgatgccgcg cgggtggaag cgccacgagg ctaaaaggga cgctccgccg agtaccgggc
     1201 cggcccccgc gccgagccct taagccagcg gcgacctatt tcatcccttc ccgcgagggg
     1261 caggtatgaa gagacgcatg gccaaagtcg gaattgccaa gtgacgctgg atgccggctt
     1321 tcaccggcat gtctctttcg aacctccccc tcatggggag gtccgacgac gccgaaggcg
     1381 gcgtcggggt ggggggcctt gcgctcaagc ggcgaagccg tcgcgcatca agcaagtgcg
     1441 gcgaggcggg tgagggtgag ttaaggacgg cgctggcgcc tgaccctcac cgcgcgctat
     1501 cgcgcgcgtg ctctccccaa ggggagagcg aagactgagg cacagcttct cctctaacct
     1561 ccccctcgtg gggaggtccg acgacgcgct ctattcttgc actcccgcgt tgctacttga
     1621 gcgcaaagcg cggcgtcggg gtgggggcct tgcgctcaag cggcgaggcc gtcgcgagct
     1681 gaagttccac cccccacccg gcgcgccacg cgctcaagta gcgaaaacgt agcgcaaaat
     1741 tccagtggcg cgccgacctc cccacgaggg ggaggttagg agcttgaggc gaatccggaa
     1801 cgctagcctg agcgttagac gcgaacctag tcgggaagat tgtcgtcgtc gtccggccct
     1861 gagaggtcct gcggcccttc gccaggcagt tcgtcgtcga tgggatcgct ggcttccggc
     1921 gtgtccgggt cgttcggcgc aatcagttcc tcgccgccac cctctccgcc gatatccagg
     1981 atgggcttgc cgctcttgcg ttcgatctcc gccccgccgc cgcccatgtc gatctcgacc
     2041 tccgagccgg gctgctcttc ctcctggatt tcctccgtca tatcgacggc aagagccggt
     2101 ccggcaacgg acacactcag tatcgccgcg agtccaacca acatgcgtcg catcggaacg
     2161 ctcctttcaa agggtcaatt caaacgggga cctttagcac ggccttcaat ccgccgagcg
     2221 gagaccggct gagcgcgatg tcgccgccgt ggatgcgggc gatatcgcgg gcgatggcga
     2281 gcccgagtcc ggtcgagccg gaatcctgat tgcgcgcatg atcgatccgg tagaaggggc
     2341 ggaacacctg ttcgcgctcg tcctcgggga tgccggggcc gtcatcctcg accgcgacgg
     2401 tcaaggtccc gttctgcttg gcggcgctga cattgacgtg ctcggcgaag cgcgcggcat
     2461 tgttgaccag attggcgatc gcccgcttga aggcgtgacg gcgcacgggg acaaccagcg
     2521 gggcttcctt gacctcgacg gcgatgtgct tggtggtgtg cgcctggctc ttcacctcgc
     2581 ccagaagctc gccgatatcg gcgcgcacga tctcctcgcc ggaatcgcct ttggcgaaag
     2641 ccatgtagtc ctcgagcatg gcctgcatct cgtcgacatc ggcgtgcatg gcttcgagtt
     2701 cgggcgtttc ctcgaacagc gcgagttgca gcttgaagcg cgtcaacacc gtgcgcaaat
     2761 cgtggctcac gcctgccagc atgatggtgc gctgctcgac atagcgctcg atgcggtcgc
     2821 gcatctcgat gaaggcatgg gccgcttggc gcacctcgcg cgcgccgcgc gggcggaaat
     2881 ccgcgggcgg cggccggccc ttgccgaagc tctcggccgc ctccgacaag cgcacgatcg
     2941 gcttgatctg gttgtgcaga aacagaatcg cgacggtcaa caggacgagc gaggtgccga
     3001 ccatccacaa cagaaagatc tgcgagttgg aggcataggt ctggctgcgg cgggcaatgg
     3061 cgtgcatcac cgcattgtcg agcttgatgc gaatatcgac gaagtttgag cggccgaccg
     3121 tgtcgatcca gaacggccgc ccgatctgac gcgtcaactc gtcggacagc gtggagtcga
     3181 gaaggtcgaa gaacggcttg gcgcgcacct gcggcagttc ttcgccgcgg acgaaccgaa
     3241 ccctcacgcc gagattctgc tcggccagcc gctcgagcgt gtcggccccc tgagggttgg
     3301 cctggtagag gtcgatgagc aggccgaggt tctgcaccgt cacggtcgac aagcgccgcg
     3361 tcaccgtctg ccagtggcgc tcgaggaaca cgaaggtgag caccgactgc aacagcacca
     3421 tgggggcgat gatgatgatc agcgcccgcg catagaggcc cttcggcatg cggtcgccga
     3481 acgcggcgag caagcgttgc agcgggcgcc cgcggcgcgc tcggggaata aggggcttcg
     3541 ggatcgcgtc catcattcgg gatagaggat gtaccccttg ccgcgcacgg tctggagata
     3601 gaccggattg gcggggtcgt gctcgatctt gcgcctgagc cggttgattt gcacgtcgac
     3661 cgcgcgctcg ccgccgctat cggcgtcctt ggcgagctcg tggcgggcga cgggcatgcc
     3721 cggccgctgg gcgaaatagc gcaacagctc gcgctcgcgc tcggtgagcc ggatcggctc
     3781 gccgttgcgc ttcagttcgc cgcgcgcgat gtgaaagatg aaatcgccca tgcggacctc
     3841 gccggtggtg ggagccgcgc cgccgcggcg gagcacgttg ctcaccctga gcaggagctc
     3901 gcgcggctcg aacggcttgg cgagataatc gtccaccccg gtctcgagtc cctcgatgcg
     3961 gtcctcgggc tcggcgcgcg cggtcagcat caggatcggc aagcgcgact cgcttctgag
     4021 cgagcgggca aaatcgatgc cggattcttg cggcatcatc acgtcgagaa tgagcaggtc
     4081 gaaggacagg ctgcgcatgg tggcgcgcgc gctggccaca tcgatcgccg tggtgacgcg
     4141 gaagccgtga tcctgaaggt agcgggcgag caggtcgcgg atgcggctgt cgtcgtcgac
     4201 cacgagtata tgcggcgcgt tgtcgtccgg gctgccgctc ttggccttga tcggtgtcgg
     4261 cgcggtcatg agcctccccc ctgggcgctc tccttgagcg ggaccggaga cgcggccatg
     4321 atagaggaaa ctttgggccg ttcttcggca ttgaccatgg cttcgagaaa gcggcggaga
     4381 tgcgcctccg ctccggggcc cgcagccttc agggcctcgg cgagccgcac cgattgcggc
     4441 gccgccagcc gctcggccag aacccggcct ttgtcggtgg gaaatagcag gcgctcgcgg
     4501 cgatcggaac gccccgcccg ctggacgata tagcctttgc cgatcagctg cttgagcacg
     4561 cgggacaggc tctgcttggt gatggtgagg atgtccagca ggtccgccac cctgagcccc
     4621 gaatggcgat tgacgaaatg cagcacccgg tgatgcgccc ggccgaagcc gaaatccttg
     4681 agaatcacgt cggggtcgcg ggtgaaatcg cgataggcga agaacaggag ctcggcgaat
     4741 tcgagcagct tcgcctctgg caggctcgtc cgcttgcggg cgcgcccatt gccgcgcggc
     4801 ttgccgcgca cggtcttcgc gcggggcgcc ttgtcgccgg ccagggtctc agttaggtca
     4861 gtcatgttga cttagattcc gccctcggtt atgtcagcca tgttgactta tttccagcat
     4921 agatacgcct cggcgcgcgc gcgcgcaatt gccctgcaaa ggtgctatca gaggcgcttc
     4981 gcccggcatt gagttccggg tcgcgccgca tcaagacggc gggcccttgt tcgcggagga
     5041 atgctcatgg tgaccgcccc ctacgatcag cgcgaggggt tcatctggct gaacggcaag
     5101 ttggtcccct ggggcgatgc gcgcctgcat gtgctgaccc acgggcttca ttacgcgagc
     5161 gcggtgttcg aggggatgcg cgcctatgag ggcgagatct tcaagctgac cgagcacgat
     5221 cggcgcctgc acgactcggc caagattctc gatttcgaaa ttccctacac ggtggccgag
     5281 ctcgacaagg cggcacgcga ggtcgtgcgc gccaacaagc tcgcagatgc ctatgtccgg
     5341 cccatcgcct ggcgcgggcc ggagcagatg ggcgtctccg cgcaactgac aaagaccaat
     5401 gtggcgatcg ccgcttggga ttggggctcc tatttcgatc cggccgcacg cgccaagggt
     5461 ttgcgcctga cctttgccga gttccgccgt cccgatccgc gcaccgcgcc ggtcaagagc
     5521 aaggcgaccg gcctctatat gatctgcaca atcgagaagc accgggcgga gcgtttgggc
     5581 tataacgacg ctctgatgct cgactggcgc ggccagatcg ccgaagcgac cggcgccaat
     5641 atcttcttcg tcaaggacgg cgcgctgcac acgccgcttc ccgattgctt cctggacggc
     5701 atcacgcgcc agaccgtgat cgggctcgcc aagaagcgtg gattgaaagt gatcgagcgg
     5761 gcgatcatgc ccgaggaact aaccacgttc tcggagtgct tcatcaccgg caccgccgcc
     5821 gaggtgacgc cggtctcgga gatcggccaa tacaagttcg ccatcggcga catcacccgc
     5881 attctgatgg acgatttcac cgcgatggtg caccccgcca aggccgttgc cgtggcgggg
     5941 tgagggggct catccaaaag tctcgacgca aggagaagac gcaatgggac gcccaccgct
     6001 gaaacaagct tataccgtct cctacgcgaa ggccggtatc gagctgaagc gctacaccat
     6061 taccgcgctc aatcaggtcg acgccgagac cgaggcggac aatcgcttca atcgcgcgca
     6121 tccggagctc aaagtctttg aggactccgt ctcccggcgt gtcgaggcgc attaacgtcc
     6181 aagctttgag gagcgagccc gcaatcctgc ccgcatccgt gctaacctct ccccatggat
     6241 cgcatggctg aagcgggatt ggccgaaaag cgggaaggca cgccggagtt cggccctgcg
     6301 ttccgcgagc agcttcacga actcctgaaa tggcgccgcg acgtgcgccg cttcaagcgg
     6361 aaaccgcttc ctcccggcag gatcgagcgg ctgatcgcga tcgcctgtct ctctccgtcg
     6421 gtcggtctct ccgagccttg gcgctttgtc atcgtcgatg acgaaagccg ccgcgccgcc
     6481 attcgggcct gcttcgaggc ctgcaacaag gacgcgctct caatccagac gcccgaccgc
     6541 gccgcgctct atgcgcggct caagctcgcg ggtctcgacg acgcgccttg ccagatcgcc
     6601 gtcttcgccg accgtgctac ggccaaaggg acgggcctcg gccgtctcac aatgcccgag
     6661 gcgctcgatt attcggcggt catcgccatc cacacgctgt ggcttgccgc gcgcgccgaa
     6721 ggaatcggcg tgggctgggt gtcgatcctc gatccgaagc gggtcgccgc gatcctcgac
     6781 gtgccggagc attgggtgtt catcggccat ctctgcgtcg gctatccgca tgcctccgac
     6841 caccggccgg cgctcgagcg cgcaggctgg gagcaccggc acccgcccgc gaccgccatg
     6901 ctttaccgat gacacctctt ccctttcccc ttggggaaag agggttttcc gccgtgatct
     6961 tgccacgaag ctcgcaagtc acatggcgga aagggtgagg ggggaaacgg cgcgtcgaat
     7021 tttgagaccc ctcacccggc acgcccgagc ttcgctcggc cgcgccaccc tctcccacaa
     7081 ggggagaggg acgagagaca cgcttgatcc gaaggccggc aacgggcact ctaggcgcgg
     7141 ggatggaggc tgacgcgagc gacctcggca cacttggtcg cgaagagccg cgggaagggg
     7201 ggcctcgatg cgcaaggcat tgctgattct cggctcggcc ggattggtgg tggcggcttg
     7261 cctcttgacg ctgcccgggg gcgaggcgca ggcgcgagtg tggaaggaat gtcagagaat
     7321 cgccttctgc agcggttgca ggccgtattt ccgctgccgt tcgtgcgagt acaggcgcac
     7381 ctgttccggc ggcctctgcg gctggggtga tgtctgcgtc tggggtccct acgtcagggt
     7441 gctgccgcgc ggcgcccgca tcatccgccg gtagtctgct gtcaccgtgc gtctcttcta
     7501 acctcctcct ggtggggagg tcgacgcgct cttgggcgcc gggtggggag cggcgcttca
     7561 tccctacaca tccctgcgag tccgcttccg cggtgtgacg aattgacccc tcgccgcgcg
     7621 ccaatggcgc gcgtgctctc ccc
//
LOCUS       DASUQP010000211        13042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1875454, whole genome shotgun sequence.
ACCESSION   DASUQP010000211 DASUQP010000000
VERSION     DASUQP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13042
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875454"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFY74_09445"
     CDS             <1..262
                     /locus_tag="VFY74_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931956.1"
                     /translation="VVALAPTKAAAGWGWWGGPRFGFGITIGPRYGYWGGYPYAPYGY
                     YGYRPYGYYGYRPYRYYAYPSYRSNYDRPYRYKRRYAYRRWR"
     gene            complement(440..781)
                     /locus_tag="VFY74_09450"
     CDS             complement(440..781)
                     /locus_tag="VFY74_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5931957.1"
                     /translation="MQKLTDESQKFGKEGFDAALRSYGELNRGLQAIAAKTADYAKKS
                     FEDGTRAFEQLLGAKSLEQAIEIQSTYAKKAYDAWVAEATKLSELYVNLARDAYKPVE
                     QVVKKAPKSVS"
     gene            1190..2383
                     /gene="gcvT"
                     /locus_tag="VFY74_09455"
     CDS             1190..2383
                     /gene="gcvT"
                     /locus_tag="VFY74_09455"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5931958.1"
                     /translation="MTPADTATTHQGLKHTPLHALHVELGARMVPFAGYDMPVQYPIG
                     VLKEHLHTREAASLFDVSHMGQIALRAKSGHLADAALALERLLPADLAGLKPGRMRYG
                     YFTNAKGGLLDDLMVAHCGDHLLLVVNASRKVDDEAHLKAALGKDCDIEVLDRALIAL
                     QGPEAEAALARLAPDCKSLKFLEARTLNIMGALCLVMRSGYTGEDGFEISTPADLARE
                     IAEELLADACVAPAGLGARDSLRLEAGLPLYGADIDESTTPVEAGLSWAIGAARRPGG
                     AHQGGFPGAETILAQIGRGAPRTRMGLRPPGSGPEARAIVRAGARLYAEADATTPIGS
                     VTSGGFGPSLDGPVAMGYVPAALAAPGTRIFAELRGKRLPLTVTALPFIMPKYRRQAA
                     RRIKC"
     gene            2377..2739
                     /gene="gcvH"
                     /locus_tag="VFY74_09460"
     CDS             2377..2739
                     /gene="gcvH"
                     /locus_tag="VFY74_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5931959.1"
                     /translation="MLKFTEDHEWLKLEGDVATVGITAHATELLGDIVFVQLPDIGAK
                     LNKGDGAGVVESVKAASDVYAPLTGEVVEINETLVDNPATVNADPQGAGWFFKMKLAD
                     VKAIDGLMDEAAYRKHIA"
     gene            2752..5604
                     /gene="gcvP"
                     /locus_tag="VFY74_09465"
     CDS             2752..5604
                     /gene="gcvP"
                     /locus_tag="VFY74_09465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017263318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX5931960.1"
                     /translation="MDVYSTYDPYDFANRRHIGPSPEEMAEMLRVVGAPSLETLIDET
                     VPGDIRQKTPLEFGRPLSERGVLDRMRATVAKIRVLTSLIGQGYHGTIMPPAIQRNIL
                     ENPAWYTAYTPYQAEISQGRLEALLNFQTLVSDLTALDVANASLLDEATAAAEAMALA
                     RRVSKSKATAFFVDAMCLPQTIAVLKTRAEPLGWEIVIGDPFKDLNSEAVFGALFQYP
                     GVCGAFHDFAPLIDTLHDAKALAVVAADPLALTLLKPPGEMGADIAVGSMQRFGMPMG
                     YGGPHAGYIAVKDQLKRALPGRIVGISVDARGNRAYRLALQTREQHIRRESATSNICT
                     AQVLPAVIASMYAVFHGPKGLKAIAERVHRKAVRLAAGLASLGFKIAPASFFDTITVE
                     VGPYQGLIMKNAVDYGVNLRKVGHDRIGITVDERTRPATLEAVWRAFGGYDLVYRDEW
                     PEPRLPKDLIRQSAFLTNPVFHMNRAESEMTRYMRRLADRDLALDRAMIPLGSCTMKL
                     NATAEMLPITWPEFAEIHPFVPPDQAQGYAEMIEGLSKILCQITGYDAISMQPNSGAQ
                     GEYAGLLTIRAYHKARSEAHRNICLIPASAHGTNPASAMMCGMKVVVVATDKDGNIDL
                     ADLRARAEANAANLAAAMITYPSTHGVFEATVREICAIIHANGGQVYLDGANMNALVG
                     LARPGDMGADVGHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFLPGHSETGGSDLTVS
                     AAPYGSGSILPISWSYCLMMGGAGLTQATRVAILNANYVARRLGTAYGVLYTGKNGRV
                     AHECILDTRPLNERAGVTVDDVAKRLIDCGFHPPTMSWPVAGTLMVEPTESETKAELD
                     RFCDAMLAIREEARAIEEGRMDRTNNPLKNAPHTVEDLVGEWNRPYSREEACFPAGAF
                     RVDKYWPPVNRIDNAYGDRNLVCVCPPVEAYAKAAE"
     gene            6033..6425
                     /locus_tag="VFY74_09470"
     CDS             6033..6425
                     /locus_tag="VFY74_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX5931961.1"
                     /translation="MATKDDPEPTSLSEDAARAGVTGHHVRAVLAFGLAGVIVAFIAI
                     AVYYGFDRLAQAFLRFASDPLSSFQNNAAYAFVIVIAAVGAVLLLGLWNRVAGQSADT
                     SQWGMRIRVVLQFAIICAAMAILYLSAR"
     gene            6655..7407
                     /locus_tag="VFY74_09475"
     CDS             6655..7407
                     /locus_tag="VFY74_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5931962.1"
                     /translation="MWDDILLYVIIGFVAQMIDGAIGMAYGVTASSVLLSLGIPPATA
                     SACVHAAETFTTGASGIAHWRFGNVDKKLLLRLAIPGMIGGAIGAYALSELPGDVLKP
                     YISGYLLLLGVFIVWKALEVRPTKVPEPRAVAPLGLVGGVVDAIGGGGWGPIVTSTLL
                     GQGTTPRYAIGSVNMAEFFVTLTISTTFFLTVGLSLWPIIVGLIIGGVIAAPFAAIAT
                     KHIPDQVLMLLVGCVVVLLSLRTIIMVLAPGQ"
     gene            complement(7435..8136)
                     /locus_tag="VFY74_09480"
     CDS             complement(7435..8136)
                     /locus_tag="VFY74_09480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX5931963.1"
                     /translation="MTHILVLQHEEIEHLAVFQDFLRADGAKWHVVELDEGETIPSLD
                     PFDAMFVMGGPQDVWEEDVYPWLRDEKAAIRRFVAEMRRPFFGICLGHQLLADALGGQ
                     VKRARVAERGLTRVSKTDAGARDPLLRDLSDPFTAFQWHGAEVVALPEGAAVLARNET
                     CPIQAFRYGSHAYGVQFHIEVTKDTVADWSQHISPVMNEAELARKVDEAYPELHALAR
                     NAYDNLKTLWARAPA"
     gene            complement(8133..9476)
                     /locus_tag="VFY74_09485"
     CDS             complement(8133..9476)
                     /locus_tag="VFY74_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5931964.1"
                     /translation="MPTGDGSLGNVLGIVAVLVLVAANGFFVAAEFSMVAVRRSRVAQ
                     LVAEGRRNAKALQRATAQLDAYLAACQLGITISSLGLGWIGEPALAHLIEPLLSGIFG
                     QFALAGAHVVAVVIAFFIITALHIVLGELMPKSLALQRSERTALAVVRPLWLFRVLLW
                     PAIVTLNGVGNWLLKLCGLRPGTAEEALHSPEELKLLIAASQEAGLLQPAQQELVERV
                     LSIGNRRITDVMTPRVDVEWIDVEDTPKETLKTIRESHHEQLLVGKGGIDEPLGTVLK
                     KDLLDQVLAGKKLDIMAALRQPIAIPEPMPIFRVLEQFKRAPVRLATVLDEYGVLQGI
                     VTQTDLLEAIAGELPEVEGEEPGIVERDDGSLLIDGMMSAPDAFDRLGFKERPEGDFH
                     TVAGFALSTFGHLPEVGESFDYEGWHFEIVDLDGRRIDKVLAIPPRAVEEEAAPE"
     gene            9784..9942
                     /locus_tag="VFY74_09490"
     CDS             9784..9942
                     /locus_tag="VFY74_09490"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEX5931965.1"
                     /translation="MPGTACADFPKVFDVGKKPWIDVDGASPEAIAAQVGKCPSGALS
                     YELLKKAK"
     gene            9976..10716
                     /locus_tag="VFY74_09495"
     CDS             9976..10716
                     /locus_tag="VFY74_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5931966.1"
                     /translation="MDDLATLFSLNNWPGHLSYVLIAISYWLTDIFWLRTVAVVGLSL
                     EILYFLLSGGDLRTGIGWDLVFILINLYQLSRLVQDRLSLRLPEEDRALLRKAFTGLD
                     DVKIARLLGAGALTDFPAGATLAEENQALDKLYFICDGHVRVTIAGRAVSELEKGNFV
                     GEVAFLTERPATATVVAETPLRALAFERDRLKQFFKNETEVAGLIYQLIGRELAHKIK
                     LSNTLIAGEGVVAAPLASEPLATGRTPA"
     gene            complement(11034..11288)
                     /locus_tag="VFY74_09500"
     CDS             complement(11034..11288)
                     /locus_tag="VFY74_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5931967.1"
                     /translation="MEWTQAAGSWKQAKIKIQRKWGKLSDDDLDLIDGRRDRLERKIL
                     QLYGFAPDHVRKEVDDWVRWQGLAGPCNRKAQLRSELALG"
     gene            complement(11417..11641)
                     /locus_tag="VFY74_09505"
     CDS             complement(11417..11641)
                     /locus_tag="VFY74_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931968.1"
                     /translation="MAPASTRTCISLFVAGVLTLTLAACASQPTFVAADYAKCQELGF
                     QSGSRDYAICLTEVQKRRSTLAAAPEPIVE"
     gene            complement(11644..12669)
                     /gene="mepA"
                     /locus_tag="VFY74_09510"
     CDS             complement(11644..12669)
                     /gene="mepA"
                     /locus_tag="VFY74_09510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX5931969.1"
                     /translation="MLRPAAPLCAMLMLSCGIAAAQDLPQFKSLFGSRAASATAPAKT
                     LFGAATSPAPLAPRAIGSYSRGCLAGGVALRINGPGFQVMRLSRNRNWGHPLLIAFLE
                     QLANDARALGWPGLLVGDLAQPRGGPMLTGHSSHQIGLDADIWLTPMPNRVLTRSERE
                     NMTAVSMLRDPLNVDREVWTPRHTRLIRLASSYPEVERIFVHPAIKQALCDQAGHDRA
                     WLSKVRPWWGHYYHFHVRLACPRGSASCRGQKEAARGDGCGAELDDWFARLLQAERKP
                     AEPENKADRPLTLADLPDDCHTVLTSGGGVARAAKPRTADKPAGPPPPHLRLGTGREP
                     ALFGFGD"
BASE COUNT         2331 a         4182 c         4122 g         2407 t
ORIGIN      
        1 cgttgtcgcc ttggccccca ccaaagccgc cgccggctgg ggctggtggg gcggcccgag
       61 attcggattc ggcatcacga tcggtccgcg ctacggatat tggggcggat atccctatgc
      121 cccgtacggc tattacggct accggccgta cggctactat ggctaccgcc cgtaccggta
      181 ttacgcctac ccttcgtacc ggtccaacta cgatcggcca tacaggtaca agaggcgcta
      241 cgcgtatcgg agatggcgct agcctgagcg acgggagctg cgcgggttgc gcggagctcg
      301 aatctcgaat gctcaggtga agggtgttaa agcgccgcac ggagggtgaa gcaaaagaaa
      361 agacccggag gggcagggcc tctccgggtc gtacctgaca ggcacgcttc ggcggcgcgc
      421 ctcaactcaa ctgcacgaat tacgaaacgg atttcggcgc cttcttcacc acctgctcga
      481 caggcttata ggcgtcgcgc gcgaggttga cataaagctc gctcagcttg gtcgcctcgg
      541 cgacccaggc gtcataggcc ttcttggcat aggtcgactg gatctcgatg gcctgctcca
      601 gcgacttcgc gccaagcagc tgctcaaacg cgcgggtgcc atcctcgaag gacttcttgg
      661 catagtccgc cgtcttggcg gcgatcgcct ggaggcccct attcagctca ccgtaagagc
      721 ggagagcggc atcgaagccc tctttgccga atttctgcga ctcgtccgtc agtttctgaa
      781 ttgcgtcgaa catctaacac ttgctccctt tgcaaagacc ggcgaaccgg gttggtttcc
      841 gtcgcaggtc atctatgtgc atcgcagcag gagggcaaga cctattttgt gcaatgcaca
      901 aaataggtcg gtgagggagc aaccgagagc caagaaattc tcttgtggag caatgggttg
      961 ggcggctatc tggcgcagaa tgtcgcagcc taatctgggc ctcatatcgc gccgcgcgcc
     1021 tacgcggatg ggagcggaac gcgcctcgtg attcgggtcg atccgccggc tcgcgattac
     1081 gcggcgcggg cgacaggcca tgacataata tctggtctcc agaccggcgc gatctctttc
     1141 cgcgcgccgc tagggcagcg accggagaca tcgtttcaag aggcttggca tgacccccgc
     1201 ggacaccgcg acaacgcatc aagggctgaa gcacaccccg cttcatgccc tgcacgtaga
     1261 gctcggcgcg cgcatggtgc cgttcgccgg ttacgacatg ccggtgcagt atccgatcgg
     1321 cgtgctcaag gagcatctgc atacgcgcga agccgcctct ctgttcgatg tctcgcatat
     1381 gggacagatt gccttgcggg cgaagtctgg gcacctcgcc gacgcggcgc ttgcgctcga
     1441 gcgcctcctt cccgccgatc tcgccggtct caagccgggg cgcatgcgct acggctattt
     1501 caccaacgca aagggcggcc tcctcgacga cctgatggtc gcgcattgcg gcgaccatct
     1561 gctcctcgtc gtcaatgcct cgcgcaaggt tgatgacgaa gcgcatctta aagcggcgct
     1621 cgggaaggat tgcgatatcg aagtgctcga tcgcgcgctg atcgcgctgc aagggcctga
     1681 ggccgaggcg gcgctggcaa ggctcgcgcc tgattgtaag agcctgaaat tcctcgaggc
     1741 gcgcacgctc aacatcatgg gggcgctgtg tctcgtcatg cgctctggct ataccggaga
     1801 agacggtttc gagatttcga cccctgccga tctcgcacga gagattgccg aggagctgct
     1861 cgccgatgcg tgcgtggcgc cggcagggct cggcgcgcgc gattcgctga gactcgaagc
     1921 cgggctcccc ctctatggcg ccgatatcga tgagagcacg acgccggtcg aagccgggtt
     1981 gtcctgggcg atcggcgccg cccgccgccc cggcggcgcg catcaaggcg gcttccccgg
     2041 ggccgaaacg atccttgcgc agattggccg aggcgctccg cgcacccgga tgggcttgcg
     2101 tcccccgggc tccggtcccg aagcgcgtgc gatcgtgcgc gccggcgcgc gactttatgc
     2161 cgaagccgac gcaaccacac ccatcggctc ggtcacatcg ggcggctttg gcccgagcct
     2221 cgatggcccg gtggcgatgg gctacgtccc cgcggcgctg gccgcgccgg gaacgcggat
     2281 cttcgccgaa ctccgcggca agcgactacc tttgaccgtc acggcgcttc ccttcatcat
     2341 gccgaaatac aggcggcagg ctgcaaggag gatcaagtgc tgaaattcac cgaagaccac
     2401 gaatggctca agctcgaagg ggatgtcgca accgtcggca tcaccgcgca cgcgacggag
     2461 ctgctcggcg atatcgtgtt cgtgcagctt cccgatatcg gcgccaagct caacaaaggc
     2521 gacggcgccg gagtggtcga atcggtgaag gccgcctccg acgtctatgc gccgctcacg
     2581 ggcgaagtgg tcgaaatcaa cgagaccctc gtcgacaacc ccgcgacggt caatgccgat
     2641 ccgcaagggg cgggctggtt tttcaagatg aaacttgccg acgttaaggc gatcgacggc
     2701 ctcatggacg aggccgccta tcggaagcat atcgcctgag ttgccgcccc gatggacgtt
     2761 tacagcacat acgatcctta cgattttgcc aaccggcggc atatcgggcc ctcgccggag
     2821 gagatggccg agatgctgcg cgtcgtcggc gcgccgagtc tcgagacgct gatcgacgag
     2881 actgtgcccg gcgatattcg ccagaagaca ccgctcgagt tcggccggcc gctctccgag
     2941 cgcggcgtcc tcgaccgcat gcgggcgacc gtcgccaaga ttcgcgtgct cacctcgctg
     3001 atcggacagg gctatcacgg caccatcatg ccgccggcca tccagcgcaa tatcctcgaa
     3061 aacccggcct ggtacaccgc ctacaccccc tatcaggccg agatcagcca ggggcgactc
     3121 gaggcgctcc tgaacttcca gacgctggtc tcggacttga ccgcgctcga tgtcgccaat
     3181 gcttcgctgc tcgacgaggc gaccgccgcg gccgaggcca tggcgctcgc ccgccgcgtc
     3241 agcaaatcga aggcaaccgc cttcttcgtc gatgcgatgt gcctgccgca gaccatcgcc
     3301 gtgctcaaga cccgcgccga gccgctcggc tgggagatcg tcatcggcga tccattcaag
     3361 gatctgaact ccgaggcggt attcggcgcg ctgttccaat atcccggcgt gtgcggcgcg
     3421 ttccacgatt tcgcgccgct gatcgacacg cttcatgacg ccaaggcgct cgccgtcgtc
     3481 gccgcagacc cgctcgcgct gacgctattg aagccgcccg gcgaaatggg cgccgacatc
     3541 gccgtaggct cgatgcagcg ctttggcatg ccgatgggtt acggcggacc gcacgccggc
     3601 tatatcgcgg tgaaggatca gcttaagcgc gcgcttcccg ggcgcatcgt cggcatctcg
     3661 gtcgatgcgc ggggaaatcg cgcctaccgg ctcgcactcc agacgcgcga gcagcatatc
     3721 aggcgcgaga gcgcgacctc gaatatctgc accgcacagg tgcttcccgc agtcatcgct
     3781 tcgatgtacg cggtgttcca cgggccgaag ggcttgaagg ccatcgccga gcgcgtgcac
     3841 cggaaggcgg tccgtctcgc cgccggcctc gcctctctcg gcttcaaaat cgcgccggcg
     3901 tcgttcttcg acaccatcac cgtcgaggtc gggccgtatc agggcttgat catgaaaaac
     3961 gccgtcgatt acggcgtcaa cttgcgcaaa gtcggccacg accgcatcgg catcaccgtc
     4021 gatgagcgca cgcggcctgc gacgctggaa gcggtatggc gcgccttcgg tggctacgac
     4081 ctcgtctatc gcgacgaatg gcccgaaccc cgcctgccca aggacctcat ccgccaatcg
     4141 gccttcctca ccaatccggt gttccacatg aaccgggccg aaagcgagat gacgcgctac
     4201 atgcgccgtc tcgccgaccg cgatctcgcg ctcgaccgcg ccatgatccc gctcggctcg
     4261 tgcaccatga agctcaacgc aaccgccgag atgctgccga tcacctggcc ggaattcgcc
     4321 gagattcacc ccttcgtacc gcccgatcag gcgcaaggct acgccgagat gatcgagggc
     4381 ctgtcgaaga ttctctgtca gatcaccggc tatgatgcca tctcgatgca gcccaattcc
     4441 ggcgcgcaag gcgagtatgc cggtctcctt accatccgcg cctatcacaa agcccgtagc
     4501 gaggcgcacc gaaatatctg ccttatcccg gcctctgcgc acggcaccaa ccccgcctcg
     4561 gcgatgatgt gcgggatgaa ggtggtggtc gtggccaccg acaaagacgg caatatcgac
     4621 cttgccgatc ttcgcgcaag ggccgaggcc aacgccgcga atctcgccgc cgccatgatc
     4681 acctatccct cgacccacgg cgtgttcgag gcaacggtac gcgagatctg cgccatcatc
     4741 catgccaatg gcggccaggt ctatctcgac ggcgccaata tgaatgcgct ggtcgggctc
     4801 gcgcggccgg gcgacatggg cgccgatgtc ggccatctca atctgcacaa gacgttctgc
     4861 atcccccatg gcggcggcgg accgggcatg ggaccgatcg gggtcaaggc ccacttggcc
     4921 ccgttcctcc ccggtcattc cgaaacgggc ggaagcgatc tcaccgtttc ggccgcgcct
     4981 tacggttcgg gttcgatcct gccgatctcg tggagctatt gcctgatgat ggggggcgct
     5041 ggcctcaccc aggcgacgcg ggtcgccatt ctcaacgcca actatgtcgc cagacggctc
     5101 ggcacggcct atggcgtgct ctataccgga aagaacggac gtgttgccca tgaatgcatc
     5161 ctcgacacaa gaccgctcaa cgagcgcgcc ggcgtcactg tcgacgacgt cgccaagcga
     5221 ctgatcgatt gcggcttcca tcccccgacc atgagctggc cggtcgcggg aacgctgatg
     5281 gtcgagccga ccgagtcgga gaccaaagcc gaactcgacc gcttctgcga cgcgatgctc
     5341 gcgatccgcg aggaggcgcg cgccatcgaa gagggacgca tggaccgcac caacaatccg
     5401 ctcaagaacg ccccgcacac ggtggaggat ctggtcggcg aatggaatcg cccctattcg
     5461 cgtgaggaag cctgcttccc cgccggcgcc tttagggtgg acaaatactg gccgccggtg
     5521 aaccgcatcg acaatgccta tggcgaccgc aatctcgtct gcgtgtgccc gccggtggag
     5581 gcctacgcca aggccgccga gtaggccggc gccgcgtgct tgcaagatcg cgatttgtgc
     5641 agcgcacaag cgctgttagc gaagcatcct aatcttctta aaggctggcg ccctcgttgc
     5701 tatatgaaaa ggaggactca cgcatcgaag aggcaggacg tgagcaagct caaacgcatc
     5761 caggctaagg cccgggattt ggcgcgttcg ggccgatttt tcgggtgggc tccgatcgcc
     5821 tttgaactcc gctttgagga cggcttcgag gacgcgcgcg agtggcttta ccgcgcggcg
     5881 acgcgagacg agcttgatcg tctttgccgc gaggctcggg cgtcgaacga cgcagccgcc
     5941 tgacaggctt agccgcgcga aaacttgagg cgccggatgc gctcgaggcc ggtgggcggc
     6001 gcgcgtggtc tcatgcggga gggactgcga tcatggcaac gaaggacgat cccgagccga
     6061 catcgttgtc agaggacgcc gccagagcgg gcgtcaccgg acaccatgtg cgcgcggttc
     6121 tcgctttcgg ccttgcgggt gtcatcgtcg cctttatcgc catcgccgtc tattacggct
     6181 ttgatcgcct ggcgcaggcg tttttgcgct ttgccagcga tcctctctcc tcattccaaa
     6241 acaacgccgc ttatgctttc gttatcgtga ttgcggcggt cggggccgtg cttctgctcg
     6301 ggctgtggaa cagagtggcg ggacagagcg cggacacgag ccagtggggg atgcggatcc
     6361 gcgtcgtgct gcaattcgcc atcatctgcg ccgccatggc gatcctctat ctttccgccc
     6421 gctaaatcca cttggccatg atcgtcatgc cagggccctg atcgaaccaa gcggtgcgtg
     6481 acgcaagggc gatgcccacg ctctcgacgc tggacccgag cccgccccaa gagtcttgat
     6541 ctacgggcaa atcgctcgaa aggcccgctg tgctcacttg ttcgaaggcg cgcgggtcgc
     6601 taacagtggg tcgcacatcg acagagcgtc aatcgtcgct cgggggcagt tttcatgtgg
     6661 gacgacatcc tcctctacgt catcatcggc ttcgtcgcgc aaatgatcga cggcgcgatc
     6721 ggcatggcct acggcgtgac cgcgagctcg gtgctgctca gcctcggcat acctcccgcc
     6781 accgcgagcg cgtgcgtgca tgcggccgag actttcacca ccggcgcctc gggtatcgcc
     6841 cattggcgct tcggcaatgt cgacaagaaa ctgctgctcc gtcttgccat accgggaatg
     6901 atcggtgggg caatcggcgc ctatgcgctt tcggaactcc ccggcgacgt gttgaagccc
     6961 tacatcagcg gctacctcct tttgcttggg gtgttcatcg tgtggaaggc gcttgaggtt
     7021 cgtccgacca aagtgccgga gccccgcgcc gtcgcgccgc ttggcctcgt cggcggcgtg
     7081 gtcgacgcca tcggcggggg agggtggggc ccgatcgtga cctcgacgct ccttggccag
     7141 ggcacgacgc cgcgctatgc catcggctcg gtcaacatgg cggagttctt cgtcaccttg
     7201 accatatcga cgaccttctt cctcaccgtg ggtttaagcc tgtggccgat catcgtcggt
     7261 ctcatcattg gcggcgtgat cgccgcgccc ttcgcggcga ttgcgaccaa acacattccg
     7321 gatcaggtgt tgatgcttct ggtcggctgc gtcgtcgtgc tgttgagcct gcgcaccatc
     7381 atcatggtgc tcgcacccgg tcagtgacac ttgtcgcttg gggtcgatcc cggactaggc
     7441 aggggcgcgg gcccacaacg tcttgagatt gtcgtaggcg ttgcgcgcga gcgcgtggag
     7501 ctccgggtag gcttcgtcga ctttgcgcgc gagttccgcc tcgttcatga ccggactgat
     7561 atgctgggac cagtcggcga cggtgtcttt cgtcacctcg atatgaaatt ggacgccata
     7621 ggcgtggctg ccatagcgaa aggcctggat ggggcaggtc tcgttacgcg caagcacggc
     7681 ggcgccttcg ggcaaggcaa ccacttcggc gccatgccac tggaacgcgg tgaaagggtc
     7741 gctcaagtcg cgcagcaggg gatcgcgcgc gcctgcgtcc gtcttcgaca cgcgggtgag
     7801 gccgcgctcg gcaactcttg cccgctttac ctgtccgcca agcgcatcgg cgagaagctg
     7861 gtgaccgagg caaatgccga aaaaggggcg ccgcatctcg gcaacgaagc ggcggatcgc
     7921 agccttctcg tcgcgcagcc agggatagac atcctcctcc caaacgtctt gcggaccgcc
     7981 catgacgaac atggcatcga aaggatcgag cgaaggaatc gtctcgccct cgtcaagttc
     8041 gaccacgtgc catttggcgc cgtcggcgcg gaggaaatcc tgaaagacgg cgaggtgctc
     8101 gatttcctcg tgctgcaata caagaatatg cgtcattctg gtgcggcctc ctcctccacg
     8161 gcgcgcggcg gaatcgccag caccttgtcg atcctgcgtc cgtcgagatc gacgatctcg
     8221 aagtgccagc cctcatagtc gaaggattct ccgacctcgg gcagatgccc gaaggtggaa
     8281 agagcaaagc ccgcgacagt atgaaagtct ccctcggggc gctccttgaa cccgagccgg
     8341 tcgaaggcgt cgggagcaga catcatgccg tcgatgagaa gcgatccatc gtcgcgttcg
     8401 acaatgccgg gctcttcgcc ctcgacctcc ggcaactcgc cggcgatggc ttcgagcaga
     8461 tcggtctgcg ttacgatgcc ctggagcacg ccatactcgt cgagaacggt ggcgagcctg
     8521 accggcgcac gcttgaactg ctcgagaacc ctgaagatcg gcatcggctc ggggatggcg
     8581 atcggctggc gcagcgcggc catgatatcg agctttttgc ctgcgaggac ctggtcgagc
     8641 agatcctttt tcagcaccgt gccgagcggc tcgtcgattc cgccttttcc gaccagaagc
     8701 tgctcatgat gactctcccg aatggttttg agcgtttcct tgggcgtgtc ttcgacgtcg
     8761 atccattcga cgtcgacgcg cggcgtcatg acatcggtaa tgcggcgatt gccgatcgag
     8821 agcacgcgct cgaccagctc ctgctgtgcc ggttgcagca atcccgcctc ctgacttgcg
     8881 gcaatgagca gcttcagctc ctcgggcgaa tgcaaggcct cctcggccgt gcccggcctg
     8941 agcccgcaca gcttcaacag ccagttgccg acgccgttga gcgtgacgat ggccggccac
     9001 aacaagacgc ggaacagcca aagcgggcgc accacggcaa gagccgtgcg ctcgctccgc
     9061 tggagtgcga gactcttcgg catcaactcg ccgaggacga tatggagcgc ggtgatgatg
     9121 aagaaggcga tgaccacggc aacgacatgg gcgcccgcga gcgcgaactg cccgaaaatc
     9181 cccgagagaa ggggctcgat gaggtgggcc agggccggtt cgccgatcca gccgagcccg
     9241 agcgacgaaa tggtgatgcc gagctgacaa gcggcaaggt aggcgtcgag ctgcgccgtg
     9301 gcccgctgga gagccttggc atttcgcctt ccttccgcca cgagctgcgc gacgcggctg
     9361 cggcgcaccg cgaccatgga aaattcggcg gcgacgaaga aaccgtttgc cgccaccagc
     9421 acgagaaccg ccacaatacc gagcacgtta cccaaactgc catcgcccgt aggcatgatc
     9481 cttctctttc aacctcgctg atgttggggt gaaaccccag catagactgc cagctcgccg
     9541 atgtcgcgat ccggttttgg cgaagtattc cgcggccctg ataagccgtt ttgggcgtgc
     9601 cggtctttgc ccttgcgatt caccgcaagt agcgcgggct gccgcagcgc gcggctccgt
     9661 gctattttga tccgggacgt tgtcgggggc cgctggttgc gggtagcgtt tccatggagg
     9721 ctgcgatgcc agaatatcga aacgacaaaa ttgtcgtccg ctacgacccc gagatctgca
     9781 ttcatgccgg ggactgcgtg cgcggatttt cccaaggtgt tcgatgtcgg gaagaaaccg
     9841 tggatcgacg tcgacggcgc ttcgcccgag gctatcgccg cgcaggtcgg gaagtgcccg
     9901 tccggcgcgc tcagctacga attgctaaag aaagccaagt aggcgccttg ggcgcgatac
     9961 ggtgcgggaa atgccatgga cgatcttgcg acccttttta gtctcaacaa ttggcccggt
    10021 catctttcct acgtattgat cgcgatctcg tattggctga ccgacatctt ctggctcagg
    10081 accgtggccg ttgtcggatt gtcgctcgaa atcctctact tcctgctgtc gggcggcgac
    10141 ctgcgcaccg gcatcggctg ggacctcgtt ttcatcttga tcaatctcta ccagctcagt
    10201 cgtttggttc aggatcgctt gagcctgcgg ctcccggaag aagaccgcgc gctgctccgc
    10261 aaggcgttca ccgggctcga cgacgtgaag atcgcgcggc tgctcggggc cggcgcgctc
    10321 accgatttcc cagccggagc cacgctcgcc gaggagaacc aggcgctgga caagctctac
    10381 tttatttgtg acggccacgt ccgcgtgaca atcgctgggc gcgccgtctc cgagctcgag
    10441 aaaggcaatt tcgtcggtga agtggcgttc ctgaccgaac ggccggcaac ggcgacggtg
    10501 gttgccgaga cgccgctgcg cgcgcttgcg ttcgagcgcg acaggctcaa gcagttcttc
    10561 aagaacgaga ccgaagtcgc ggggctgatc tatcagctca tcggccgcga actcgcccac
    10621 aagatcaagc tcagcaacac actcatcgcg ggcgaggggg tcgtggcggc accgcttgcg
    10681 tccgagcctc tcgctacggg acgcacgccg gcttgatacc ggcgtgcccc gttatgattt
    10741 cagcgcaggt acgtggcggt tgcggtcagc cggacctagc ttccggcacc gcccgattca
    10801 tctctacttt tccatcgtga taattgagct tgtggatcgt ttcggggcga tgaaacgcca
    10861 cggtccacac gtgccgaaag gcacagcctt ggcaaagttt gcgggaagtt tttggcaagg
    10921 ggccgcaagc ccatcacggc tatgggtcgc gtgatgcctc cgtggcgggg tcgcaagaaa
    10981 gaggcgtgcg gaaccgcgct tttcgcccgc gtcgtaggtg ctaggagccg gcgctaccct
    11041 aaggccagtt ccgaacgcaa ttgggccttg cggttgcacg ggcccgcgag cccttgccag
    11101 cgcacccaat catcgacctc ttttcgcacg tgatcggggg caaagccgta gagttgcagt
    11161 atctttctct cgaggcgatc gcgccttccg tcgatgaggt cgagatcgtc gtcactgagt
    11221 ttgccccatt ttcgctggat cttgattttg gcctgcttcc acgatcctgc agcttgcgtc
    11281 cactccattg ctttccctcc tgaaaaatga gcagggcgtc gatctggcga aatgcattga
    11341 gcggtcaggc gttccagccg cataaaaaag aaggggatgg tgtgacattg atgccacggt
    11401 cgtgccgggc cccttgtcac tcgacaatcg gctcgggggc tgccgccagg gtggatcgcc
    11461 gcttctggac ttccgtcagg cagatcgcgt agtcgcggct cccggattgg aatccgagtt
    11521 cctgacactt ggcgtagtcg gcggcgacaa acgtgggttg gctcgcgcaa gcagcgagcg
    11581 tcaaggtgag aacgcctgcg acgaaaagcg aaatgcacgt tcgcgttgac gctggcgcca
    11641 tggctaatct ccgaatccga aaagcgctgg ctcccgccca gtcccaagtc gaaggtgggg
    11701 cggcgggggc cctgctggct tgtctgccgt ccttggcttc gccgcgcggg ccacgccgcc
    11761 gcccgaagta agaacggtgt ggcagtcgtc cggcaggtct gcgagggtca aagggcgatc
    11821 ggccttgttc tcaggctcgg ccggtttccg ctccgcctgg agaagccgcg cgaaccagtc
    11881 atcgagctcg gcgccgcagc cgtcgccgcg cgccgcctcc ttctgccccc tgcaacttgc
    11941 gcttccgcgc gggcaggcga ggcgcacgtg gaaatgatag tagtggcccc accagggtct
    12001 cactttgctg agccaggcgc ggtcgtggcc ggcctggtcg cagagcgcct gcttgatcgc
    12061 gggatggacg aagatgcgct cgacctccgg ataggacgag gcgagcctga tcaatcgcgt
    12121 gtgacgcggc gtccacactt cgcgatcgac attgagcggg tcgcgcagca tcgaaacggc
    12181 cgtcatgttc tcgcgctccg agcgcgtgag cacgcggttc ggcatcggtg tcagccagat
    12241 gtcggcatcg agcccaatct gatgactgga gtggcccgtc agcatcggac cgccgcgcgg
    12301 ttgggccaaa tcgccgacca ggaggccggg ccagccgagc gcgcgggcgt cgttcgcaag
    12361 ctgctcgaga aaggcgatga gaagcggatg accccaattg cggttgcgcg agagacgcat
    12421 cacctgaaag cccggcccgt tgatgcgaag cgcgacgccg ccggcaagac agcctcgcga
    12481 ataagatccg atcgcgcgcg gggcaagcgg ggcgggagag gtcgcagcgc cgaaaagcgt
    12541 tttggcgggc gcggtggcgc tcgcggctct cgatccgaag agcgacttga actgtggcag
    12601 gtcttgtgcc gcggcaatgc cgcagctcag catcagcatc gcgcaaagag gagctgccgg
    12661 ccgtaacatt cctggaaccc ccgaaactgt tcttattaga cggcgtgccg cctttccgag
    12721 ggtttaacgt cggggccgcg ccaaagactg ccggaacgcg tcataccggc gaaagccggt
    12781 atccagtttc cccgctctcc ccttggagag agcacgcgcg ccctttggcg cggggtgagg
    12841 ggtcaggcgc gaagcgccgt ccttatcgcc ccatcacccg gctcgccgct tgatgcggct
    12901 cgcccacctc tccacgacct gcctgcgcga agccgaagct tcgctccggc gaaggcaggg
    12961 ggagagggga aaggcagtgc gccgttgtcc gctctcgatt ttggattgcc ccgaaggatg
    13021 aagttttcag ccccgcggcc gg
//
LOCUS       DASUQP010000212         5193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_104284,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000212 DASUQP010000000
VERSION     DASUQP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104284"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFY74_09515"
     CDS             complement(<1..640)
                     /locus_tag="VFY74_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5931970.1"
                     /translation="MGRILRIFLAGVLALLPIAVTFFVVAWAASLVATFAGPGSFIGR
                     LITTLGTSFGLNIEPSSSAAYLLGLAVILGAIFLLGLLVESGLRSFILNSLDWLMTRI
                     PLVSNVYNLSKRFVAIVDRGDGEDSLQSMRPVWCFFGGEGSAAVLALMPSPEPVVIGE
                     RSYLGLLVPSAPVPFGGALIYVPSEWVMPAEGGVERLMNVYVSMGVTPPLSLP"
     gene            complement(711..2756)
                     /locus_tag="VFY74_09520"
     CDS             complement(711..2756)
                     /locus_tag="VFY74_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEX5931971.1"
                     /translation="MKSQWNDRAAQEMVAAYAEKGVGLDIALRVYTTRLLGRDPLLVL
                     HGGGNTSVKTRASDDLGEEHDVIAVKGSGADMADIEPWGLPAVKLEPLKRLRALDALS
                     DEAMVNVQRLNLLDSSAPNPSVETLLHAFLPHKFVDHTHAASVLSLVDQPDGEALARE
                     VYDGRMGIVPYIAPGFGLAKAAAQVFEAKPDVEGLVLHKHGIFTFGASAREAYERMIE
                     MVSLAESRLRQGRPIVFHARPLANASASAAEIAPIIRGAVAIAAEGAEPIRFIADFRT
                     APEILSYVNGAELASYSQRGVVTPDHIIRTKNKPLLVPQPEPGRLEAFSGAVREAVAT
                     FAADYDRYFDRENAAAGATKTKLDSAPRVVLVPGVGLFGLGRTAKDASIAAELAENTI
                     RVVTDAEAIGRYEPLPESDLFALEYWSLEQAKLKGAVVKPLTGQVALVTGAGAIGAAT
                     AKALAAEGAAVGIVDIDGEAARSAGAALKGLGLQCDVTKPDQVKAAFAALSERFGGID
                     ILVSNAGAAWQGRIGEVSDETLRKSFELNFFAHQTVAQEAVRIMLKQGTGGVLLFNLS
                     KQAVNPGANFGPYGLPKAATMLLMRQYALDYGAEGIRSNGVNADRVRSGLLTDAMIMD
                     RAKARGVSEAEYMSGNLLHAEVTAADVAQAFVALAKARKTTGHVKTVDGGNIAAALR"
     gene            complement(2796..3770)
                     /locus_tag="VFY74_09525"
     CDS             complement(2796..3770)
                     /locus_tag="VFY74_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5931972.1"
                     /translation="MERRAGLEGEMPAGRIVAKEPHQPSDRSSQAFRLADGRQIGFAE
                     YGDPGGLPVLALHGTPGSRFMFALTDRGARERRLRIIAPERPGYGLSDFQRQDSLAQA
                     AADLLECADAFGLDRFALIGVSGGAPYAVAAAAAMPDRVALLALINPVGAIAECHRRI
                     RLSKFHRLIFTRLGRSDAACTAFFWSLRSLVRTAPGLAYRALKQRVPRADRMVLEREE
                     VRANLRAVLQEGLRSGIEGARQDLRLYCTNWGLALRDIDVPAVLWQGSDDAIVPPGAA
                     YDLAETLPNCRLDVIQGAGHYWIFTAFERVLDAARAALIDPMPRRGTP"
     gene            3834..4472
                     /locus_tag="VFY74_09530"
     CDS             3834..4472
                     /locus_tag="VFY74_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018013293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX5931973.1"
                     /translation="MTEWEIKGREFGNCNCSYGCPCQFNALPTHGHCHGFGVYEIERG
                     FHGSTRLDGLRAAAIIRWPGPIHEGKGECVHVLDRRASVEQRAALLRIFRGEDTEPGA
                     TVFQVFASTCDTVHEPIIADIDFAIDVDGRNASAKIEGVLAAKGAPILNPVTGAPHRV
                     RIVQPDGFEFAEAEIGRGWSKTMGPTRFELNDSYGQFAEIHLCQNGMVRRER"
     gene            4469..4606
                     /locus_tag="VFY74_09535"
     CDS             4469..4606
                     /locus_tag="VFY74_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931974.1"
                     /translation="MTGTAVETLFRRNDAVVLAALVTLVLLFWLALFAGAGTGIDPEP
                     S"
     gene            complement(4642..>5193)
                     /gene="rpsD"
                     /locus_tag="VFY74_09540"
     CDS             complement(4642..>5193)
                     /gene="rpsD"
                     /locus_tag="VFY74_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5931975.1"
                     /translation="PKSPYNKREYGPGEHGQRRRGKLSDYGQQLRAKQKLKGYYGNIT
                     EKQFKAIYREAARLRGDTSEKLIGLLERRLDAIVYRAKFVPTVFAARQFVSHGHVRVN
                     GRRLTVPSYRCREGDVIEVRDKAKDAGMVLESAQSPERQVPDYLEVDHNKMTAKLARV
                     PTLSDVPYPTIMEPNLVIEFYSR"
BASE COUNT          896 a         1698 c         1752 g          847 t
ORIGIN      
        1 gcggcagcga cagcggcggc gtcacgccca tcgacacgta gacgttcatc agccgctcca
       61 cgccgccctc ggccggcatg acccactccg acggcacata gatcagcgcg ccgccgaacg
      121 gaaccggcgc cgatggcacg agcaggccga gatagctccg ctcgccgatc acgaccggct
      181 cgggcgacgg catcagcgcg agcacggccg cgctcccctc cccgccgaag aagcaccaca
      241 ccggacgcat gctctgaaga ctgtcctcgc cgtcgccgcg atcgacgatg gcgacgaagc
      301 gtttggacaa attgtagacg ttggagacga gcgggatgcg cgtcatcaac caatcgaggc
      361 tgttcaagat gaacgagcgc agtcccgact cgacgagaag gccgaggagg aaaatggcgc
      421 cgagaatgac ggcgaggccg agaagataag cggcgctcga gctcggctcg atattgaggc
      481 caaaagacgt tccgagcgtc gtgatgagcc ggccgatgaa gctgccggga ccggcaaagg
      541 tcgcgacgag ggaagccgcc caggcaacca cgaagaaggt gacggcgatc ggcagaaggg
      601 cgaggacgcc cgcaaggaag atgcgtagga tgcggcccaa ctcgtcctcc cgcgatggat
      661 cggcgcacga ggggccggag aatcgccgcg gcgcgagccg ccgtcaaccg ttagcgcagc
      721 gcggcggcga tattgccgcc gtcgaccgtc ttcacatgtc ccgtggtctt gcgcgccttc
      781 gccagcgcga cgaaggcctg ggccacatcg gcggcggtga cctcggcgtg caggagattg
      841 ccgctcatat actcggcctc cgacacgccg cgcgccttgg cccgatccat gatcatggca
      901 tcggtgagga gcccgctcct cacgcggtcg gcattcacgc cgttcgagcg aatgccttca
      961 gcaccgtagt cgagagcgta ttggcgcatg agcagcatcg tggcggcctt gggcaggccg
     1021 taggggccga aattcgcgcc ggggttcacg gcctgctttg agagattgaa cagaagcacg
     1081 ccgcccgtgc cctgcttcag catgatgcgc accgcttcct gcgccaccgt ctgatgggcg
     1141 aagaaattga gctcgaagct cttgcgcaag gtttcgtcgg agacctcgcc gatccggccc
     1201 tgccacgccg cgccggcatt cgacacgaga atgtcgatgc cgccgaagcg ctctgagagc
     1261 gcggcgaagg ccgctttcac ctgatcgggc ttggtcacgt cgcattgcag cccaaggccc
     1321 ttgagcgcgg cgcctgcact ccgcgcggcc tcgccgtcga tgtcgacaat cccgaccgcc
     1381 gcgccctcgg cggcaagcgc cttcgccgtg gcggcgccga tcgcgcccgc gcccgtcacg
     1441 agcgcgactt gtcccgtgag aggcttcacg accgcgcctt tgagcttcgc ctgttcgagg
     1501 ctccaatatt cgagcgcgaa gagatcgctt tcgggcagcg gctcgtagcg gccgatggcc
     1561 tcggcgtcgg tgacgacgcg gatcgtgttc tcggcgagtt cggcggcgat gctcgcatcc
     1621 ttcgccgtac ggccgagacc gaacaggccg acgcccggca cgagaacgac gcgcggggcc
     1681 gaatcgagct tggtcttggt ggcgccggcg gccgcgttct cgcggtcaaa atagcgatcg
     1741 taatcggcgg cgaatgtggc cacggcctcg cggaccgcgc cggagaaggc ttcgagcctg
     1801 cccggctcgg gctgcggcac aaggagcggc ttgttcttcg tcctgatgat gtggtccggc
     1861 gtcaccacgc cgcgctggct gtaggacgcg agctcggcgc cgttcacgta gctcaggatc
     1921 tccggcgccg tgcggaagtc ggcgatgaag cggatgggct cggcgccctc ggctgcgatg
     1981 gcgacggcac cgcggatgat gggagcgatc tccgccgctg aagcgcttgc gttggcgagc
     2041 gggcgcgcat gaaaaacgat ggggcgtccc tgcctgagcc ggctctcggc taaagacacc
     2101 atctcgatca tgcgctcata ggcctcgcgc gcgctcgcgc cgaaggtgaa gatgccgtgc
     2161 ttgtgcagaa cgaggccctc gacgtcgggc ttcgcctcga acacttgcgc ggcagccttc
     2221 gccaggccga agcccggcgc gatatagggc acgatcccca tgcgcccgtc atagacctcg
     2281 cgcgcgagag cctcgccgtc tggctgatcg acgagcgaca aaacggaagc ggcatgcgtg
     2341 tggtcgacga atttgtgtgg aaggaaggca tgcagcagcg tctcgacgga cgggttgggc
     2401 gccgaggaat cgagcaggtt cagccgctgc acattgacca tcgcctcgtc ggagagcgcg
     2461 tcgagcgcgc ggagccgttt gagcggctcg agcttcaccg cgggcagtcc ccacggctcg
     2521 atatcggcca tgtcggcgcc ggagcccttg acggcgatga cgtcgtgctc ctcgccgaga
     2581 tcgtcgctcg ccctcgtctt gaccgaggtg ttgccgccgc catgcagcac gagaagcggg
     2641 tcgcgcccga gaaggcgcgt ggtgtagacg cggagcgcga tgtcgaggcc gacgcctttc
     2701 tcggcgtagg ccgccaccat ctcttgcgcc gcgcgatcgt tccattggct tttcatggcg
     2761 cgccttgttg caagtttctc cccttccggc aaggactaag gggtgccgcg gcggggcatg
     2821 ggatcgatga gggcagcgcg cgctgcgtcg agcacgcgct cgaacgcggt gaagatccaa
     2881 tagtggccgg cgccttggat gacgtcgagc cggcaattgg gcaaggtctc ggcgagatca
     2941 taggcggctc ccggcggaac gatggcgtcg tcgctgccct gccaaaggac ggccggcacg
     3001 tcgatgtccc tcagcgcaag accccaattt gtgcagtaca ggcgcaagtc ctgacgcgcg
     3061 ccttcgatgc cggacctcaa gccctcttgc agcactgccc gaagattggc cctgacctct
     3121 tcgcgctcga gcaccatccg gtccgcgcgc gggacacgct gcttcagcgc gcgataggcg
     3181 aggccaggtg ccgtgcgcac gaggctgcgg agcgaccaga agaaggcggt gcacgcggcg
     3241 tcggagcggc cgagccgagt gaaaattagg cggtgaaact tcgacaagcg gatgcggcga
     3301 tgacactcgg caatcgcgcc aaccgggtta atcagcgcga gcagcgcgac gcgatccggc
     3361 atggcggcag ccgcggcaac ggcatagggg gcgccgccgg agacgccgat gagagcgaaa
     3421 cggtccaagc cgaacgcatc ggcgcattcc agaaggtcgg ccgccgcttg agcgaggctg
     3481 tcctgccgct gaaagtccga gagcccgtag ccggggcgct ccggcgcgat gatccgcaag
     3541 cgcctctcgc gcgccccccg gtcggtcagg gcgaacatga agcgcgagcc cggcgtgccg
     3601 tgcagagcga ggaccggcag ccctcccgga tcgccatatt cggcaaagcc gatctgccgt
     3661 ccatcggcga ggcggaacgc ctgcgagctt cggtcggatg gttggtgagg ctctttcgcg
     3721 acgatgcggc ccgccggcat ctcaccctca agcccagccc gccgctccat cgccataatg
     3781 ctccgcgctt tcctggttag acttgagcat catggtacga aggaggcagc gcagtgacgg
     3841 aatgggagat caaggggcgc gagtttggca attgcaattg ttcctacggt tgcccatgcc
     3901 aattcaatgc gttgccgacc cacggtcact gtcatggctt cggggtatac gagatcgagc
     3961 ggggcttcca cggatcgacg cggctcgacg ggctgagggc tgccgcgatc atccgctggc
     4021 ccggccccat tcatgaagga aaaggcgagt gcgtgcatgt gcttgaccgc cgcgcgtcgg
     4081 tcgagcagcg cgcggccctt ctccgcatct tccggggcga ggacaccgag cccggagcga
     4141 cagtcttcca agtgttcgcc tcgacctgcg acaccgtcca tgagccgatc atcgccgata
     4201 tcgattttgc gatcgacgtc gatggccgca acgccagcgc caagatcgag ggcgtgttgg
     4261 cggcgaaagg cgcgccgatc ctcaaccccg tgaccggcgc tcctcatcgc gtgcgcatcg
     4321 tgcagcccga tggcttcgag ttcgccgagg ccgaaatcgg gcgcggctgg tcgaagacga
     4381 tggggccgac ccgcttcgag ctcaacgaca gctacggaca gttcgcagag attcatctct
     4441 gccagaacgg catggtgcgc cgagagcgat gaccggcacg gccgtcgaaa cgctgttcag
     4501 gcgcaatgac gccgtcgttc tcgcggcgct cgtcaccctc gtgctgcttt tctggctcgc
     4561 cctcttcgcc ggggcaggca ccggcataga cccagagccg tcctgattgc cggcgctatc
     4621 ctcctcgccg gtctgtaacg cctagcgcga gtagaactcg atgacgaggt tcggctccat
     4681 gatcgtggga tagggcacgt cggagagcgt cggcacgcgc gcgagcttcg ccgtcatctt
     4741 gttgtggtcg acctccagat agtcgggcac ctggcgctcg gggctctgag ccgattcgag
     4801 caccatgccg gcatccttgg ccttgtctct gacctcgatg acatcgccct cgcggcagcg
     4861 atagctcggg acggtcaagc gccggccgtt gacccgcaca tgcccgtggc tcacgaattg
     4921 ccgcgccgcg aacacggtcg gcacgaactt ggcacgatag acgatggcgt cgagccggcg
     4981 ctcaagcagc ccgatcaact tctcggaggt gtcgccgcgg agccgcgccg cctcgcgata
     5041 gatcgccttg aactgcttct cggtgatgtt gccgtaatag cctttgagct tctgcttggc
     5101 gcggagctgc tggccgtaat cggaaagctt gccgcgccgg cgctgtccgt gctcgcccgg
     5161 tccatattcg cgcttgttgt acgggctttt cgg
//
LOCUS       DASUQP010000213        18052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_377741,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000213 DASUQP010000000
VERSION     DASUQP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377741"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..279)
                     /locus_tag="VFY74_09545"
     tRNA            complement(203..279)
                     /locus_tag="VFY74_09545"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:His,seq:gtg)
     gene            complement(512..1003)
                     /locus_tag="VFY74_09550"
     CDS             complement(512..1003)
                     /locus_tag="VFY74_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HEX5931976.1"
                     /translation="MSQANSPQAVAGAARDTFRKAAKEFESFKLDATVPESVRALAEK
                     TVNQSREAYERGKDALEEAVDALERSFDAAGQGATAFNRKLIDLAQRNLNSAFDLAKS
                     LAGAKNVAEIVELQSAFIRRQFDVFASQANEIRALSTKIAADTAEPIKSQVARSFEQV
                     RKS"
     gene            1300..2571
                     /locus_tag="VFY74_09555"
     CDS             1300..2571
                     /locus_tag="VFY74_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5931977.1"
                     /translation="MSSLPLHMPDLKPNRRADEPDKDREALEHIARALESRRFEPPAE
                     GASVGEAAEVLLTQQSLNSLLESMPAAAGILFGGTLAHANSAFANAFGYPSTAELIEA
                     GGLRAILGEGKGLGEVRSEPSAPVEALTRSRRRVKVAFALSVLDAERELTLLRLIEPP
                     ILAGEAEAQASPVGVPPKPEPAPRPPADAASSRLDFLAKVSHEVRTPLNSIIGFAELM
                     LQERFGPIGSRRYKGYVEDIHQSGLYALSLLNDLLDISKIEAGKFELNFTAVDVAEIV
                     EDCAASLQPLAKRARILLRTSLAPDLPTVVADPRRLKQCLLNLLTNAIKFTNEGGQVI
                     VSGSMVDGELRLRVRDNGVGMSKDEIAFAMQPFHQLDTAPRKQTGTGLGLPVTKALVD
                     ANRARLVLSSEPGVGTSADVIFPAERLFTSG"
     gene            2875..3918
                     /locus_tag="VFY74_09560"
     CDS             2875..3918
                     /locus_tag="VFY74_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5931978.1"
                     /translation="MRFASARFLALATLLAFAALQADAADPGEVNIYSYRQPYLIEPL
                     LKEFTEETGIKVNVIFAEKGLIERIEAEGRNSPADVLLTVDIGNLTQATAAGIAQPIR
                     VDAIDAAIPEAYRAEDDQWFGLTRRARVIYASKERVKQDSITYEELADPKWRGKICTR
                     SGQHPYNVALIASIIAHHGEAWTEDWLRGVKANLARKPAGDDRLQVKGVYAGECDIAI
                     GNTYYMGAMLKNDKQPEQKQWAGSVNILFPNTGDRGTHVNVSGAVVAKHAPHKDNAVK
                     LVAFLASDKGQQLYAEANNEYPVKEGVPWSPLVKSWGEFKPDPISLDEIARLRKTASE
                     LVDKVEFDEGPSS"
     gene            3929..4462
                     /locus_tag="VFY74_09565"
     CDS             3929..4462
                     /locus_tag="VFY74_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931979.1"
                     /translation="MSFQKKVDRQGSVRVAAWDRIPPRSGAEWLVGMGFRCWLAGYDT
                     GDIACWENGWNEYARALGPERARRAVTELACWVRAVRASASRKIEYSPVGCPGFCADE
                     CLAISLIAASQHHRCPAMRACALALTGSDSVDPVLKAANAFADALQEADQRLSPEAVA
                     TLMAGRRKGGADRLALG"
     gene            4489..6210
                     /locus_tag="VFY74_09570"
     CDS             4489..6210
                     /locus_tag="VFY74_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5931980.1"
                     /translation="MALALSSERPQWRRRLARLKDSGNLGWSAAALLISLIALLPILA
                     IGGIALKTSGDTWPHLIENVLPGALRRTALLLAGVGALTLLIGTATAWLVTMYRFPGR
                     NAFQWLLLLPLAIPTYIIAYSYLELLDYSGVVQSGLRALFGWQDARSYWFPDIRSLGG
                     AILVMSAVLYPYVYITARASFIAQSVCILEVSRSLGRTAPQTFWEVALPLARPALAAG
                     VALALMETLNDIGAVGFFGVRTLTVAIYDTWLDRNNLAGAAQIACVMLLFVFALLGLE
                     LALRGKRRFHHTTGKYRRLPKDRLEGLGGALASLACAVPVLFGFALPAGVLFYDALAH
                     SASGLSPAFWRAALNSLLLAGAAAVLAVGFAVVLAYARRQTRSKLIQGASTIPAISYA
                     VPGTVLAIGLLIPLASLDNFVDSLTRSLLGVTTGLLLSGTAFVIVLAYTIRFLAASLG
                     AVEAGLSRVSRNIDAAARSLGATLGEMLVKVHLPLLRPALGAAALLVFVDSMKELPAT
                     LLLRPFNFDTLATQVFTLVSLYRYEEAGLSALTIVLVSLAPVLLLHGLVAGSRPGASI
                     AAGREGP"
     gene            6372..8159
                     /locus_tag="VFY74_09575"
     CDS             6372..8159
                     /locus_tag="VFY74_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5931981.1"
                     /translation="MKPRQIAWKWLLLGVFVLFLAGLVILPRLFGDSFSLANRVSGAI
                     AAWTGGEVKLTGPLKLQYFPDVAIRSGFELTRASRLPLVESISAKEARISLDLAALFL
                     GRIHVDAVRLIRPEITLKEAPSLVTGSERTLQARVANVLTDAPFRVLRVRAGSIRIST
                     PSGGETIKTFNARFELAPGTRALSSSGSFVLRNETVAFALHSDMPAETADGLTVPVRL
                     AFNAPTIGATATGTASFTNRFHLDGDLKADMADARAFLRWAGIPLAEGESLKNLSASG
                     GAQWNGTTLIFDDGAFALDGNAAVGVLAVTPGKRPRIDGTLAFDRLALDPYLTSRAAR
                     PDQSALSHFDTDLRISAADITAPQVKLGRGGFTISAREGLVSTEVGELEVCGGTVTGR
                     IDVDLSNAINKSTLAGKLSDVPIEDCLGPLGLQVPLSGVGSLKVEFSTEGSNYDELAR
                     GLGGPFKLRARNGVVSVDLARLFAGSGPIEGEGWSSDNVTAFEDLIAECRLGAGHIWC
                     EKFNMKTERGLISGSGDVDLGQKTLDWRLFVADHAQPLKASQLSAETPPQISISGSLA
                     QPVIRRADGRGLGTGSVPAATKATQVSPR"
     gene            8201..9427
                     /locus_tag="VFY74_09580"
     CDS             8201..9427
                     /locus_tag="VFY74_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5931982.1"
                     /translation="MSQHRTFRFCRALALAGMNARACALPLALAAGLGASASALAEDT
                     LNIATWGGAYGQSQEIAFFEPYAKETGTRIATEIYDGTLAKIKEIIEGGSSAIDVADV
                     SAGTLNALCNDGLLETIAASSLTAAPSGESAEQDFFPGSLSACGVASVAWSTAIAFDR
                     QAFSKASPSDIANLLDTKRFPGKRALPNSAHRTLELALLADGVDPANVYTELATQAGA
                     DRAFQALDKIKAEIYWWDSTEEPMTWLLEKKAAMAAAYSGRMFRAMVGTRQRFGVIFN
                     GQVYDLDLWAIPKSARNKEGALRFIAFATDPGRLAVQAQLIAYGPMRKSALALVGKHP
                     VVGVEMLAFLPTAPDNFKKALKFDEGWWDTNGAALQSRFEAWRGPVAPGQGKPPIVQA
                     KPQEAEASPRRAKALP"
     gene            9880..10893
                     /locus_tag="VFY74_09585"
     CDS             9880..10893
                     /locus_tag="VFY74_09585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEX5931983.1"
                     /translation="MTPERFESWLTVDRKGLFCRPGAFHIDPYGGAKRAVVTHGHSDH
                     ARPGHDHVLATRETLAVMAARLGTAARASSQAASYGERVTIGDVAVTLFPAGHVLGSA
                     QVLIEWKGARVVVSGDYKRSADPTCPGFEVVPCDLFITEATFALPVFQHGEAREEVAK
                     LLASRAQFPDRAHVVGVYGLGKCQRLIALLREAGYDRPIYLHGALAECCALYESFGVR
                     LGALKPATGAPRDALKGEIVLAPPSAIADRWSRRMPDPVTAFASGWMRVRARARQRGV
                     ELPLVISDHADWRELTETIADTGAGEVWVTHGREDALLHHIQKTGRKGRALALVGRED
                     EDQ"
     gene            10890..12473
                     /locus_tag="VFY74_09590"
     CDS             10890..12473
                     /locus_tag="VFY74_09590"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEX5931984.1"
                     /translation="MKAFAALLDRLAYTPSRNDKLRLLADYFASTPDPDRGFALAALT
                     DGLFFRLPFRRILTELLQPRVDPVLFDFSRDYVGDTAETIALIWPSGRAHAKPPRLSA
                     VVEALQSASPAEVPALLANWLNQLDSTGRWALLKLLTGALRVGVSARLAKTALAEWSG
                     VALADIEEVWHGLRPPYGELFAWLSGKGPRPALEGRATFRPLMLSHQIDDKELQAIAP
                     NDFIAEWKWDGIRVQAARQGGEKRLFSRTGDDISGAFPDVVEALPEGVVLDGELLVMR
                     DGEVAPFNDLQQRLNRKIVSGALLKSHPAHIRLYDILFEGGEDLRALPFVARRARLES
                     WHKTHAPRRTDLSTLIDFTSVQELQTLWSGARETGIEGLMLKRRDSPYLAGRPKGHWY
                     KWKRAPLVIDAVLMYAQRGSGKRSSYYSDYTFGAWRDGEAGEEELVPVGKSYFGFTDE
                     ELKELDRWVRNHTVDSFGPVRQVEPKLVFEVAFDSVHRSTRHKSGVAMRFPRINRIRW
                     DKPAAEADRLETVQAMIVD"
     gene            12652..12912
                     /locus_tag="VFY74_09595"
     CDS             12652..12912
                     /locus_tag="VFY74_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931985.1"
                     /translation="MRRFAILTLVAGMLVLAAGPAQAQDVAICFRTADRVVSGEDIDD
                     AAKKAGHEACQRALAETSSVVQKYHLQEADFDIVGRPPKAPN"
     gene            complement(12926..14593)
                     /locus_tag="VFY74_09600"
     CDS             complement(12926..14593)
                     /locus_tag="VFY74_09600"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HEX5931986.1"
                     /translation="MLIKLTGGKVYDPANNVSGETRDIYVEDGKIVAARPNARADATY
                     DVKGRIVMAGAIDPHTHIGGGKMTIARMLLPEDHRGDPVTRTDLTRAGSGRAVPSTHV
                     TGYRYAEMGYTACFEPAMLPANARQAHMEMGDTPMVDKGAFVMLGSDDYFLRQLAAKK
                     DFKAIKDYVAWTLHAAQAIAVKVVNPGGISAFKFNQRKLDLDEEHVYYHVTPRQILLT
                     LARAIEELGVTHPLHIHGCNLGVPGNIETTLATVRAAEGLRIHMTHTQFLSYGIEGDR
                     KFSSGAARLTELVNKTPNASIDVGQVLFGQTCTASGDSMRQYAISKSANPKKPVIMDI
                     ECDAGCGVVPIRYRDKSFVNAMQWVIGLETFLLFDDPWRIFLTTDHPNGAPFYFYPHL
                     IRLLMDKGFRDDMLQKINPDAQAQSELLKSLTREYTLDEIAILTRAGPARSLGLKDRG
                     HLGVGASGDITVYRDDPDREAMFATPEFVFKNGEVVVKNGKVVKVVQGATHVVRPDYD
                     KAIEKPLNDYFDRYHTVRMENFKLADEEIIGGDGGAIIVQPTAARAS"
     gene            complement(14610..15911)
                     /locus_tag="VFY74_09605"
     CDS             complement(14610..15911)
                     /locus_tag="VFY74_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HEX5931987.1"
                     /translation="MPRSSPGQAKAEHPGSYLFENVVNPFCGTLCDDLVVERTKTGLK
                     VAKNSSSQSIAGFERKLPPANPSVKGKDVALAAAVKEAAKLIGKAKLPLFGGLATDVE
                     GMRAVLALAERAGGIVDHALSAVGERNLKILQTSGWITTTLTETRNRADLIIIAGSDI
                     HRLHPRFFERIISPPDSMFGVTAPKRTVVFIGKGLDRSGAKGSKIGDVVTIGCNDEDA
                     GHVVALLRARLRGFKVAPRKQSGVTLAEIDALAERCRKAAYGVVVWAPPSLDFPHAEL
                     AVEQFTGLVKDLNPTVRFAGLSLGGAEGAPTAASVSTWISGYPLRVSYASGAPDYDPY
                     RWDIGRMLGDGEGDLLVWVAAISPDLAPPPTKIPTIVLGTPGLALAAPPAVFVPIGTP
                     GLDHAGRLIRVDNVMSLPLKDLGRAELPSAAEMLSAIEAAL"
     gene            complement(16009..16728)
                     /locus_tag="VFY74_09610"
     CDS             complement(16009..16728)
                     /locus_tag="VFY74_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5931988.1"
                     /translation="MPPRILKHASRLVAAASLAGILTGPGAARAEVPASPCSEDVMIV
                     FDASGSMGGNLEQGIATLKPRIDEVRAALAEILPAVSRVRRMGLITYGPGLGHQCNVK
                     LDLTPTPNASELILRKLEGISPAGKTPLTRAVAQAAEVLDYRRHPGVIVVLTDGEETC
                     GASPCELGKELSAAAAQLTVHVIGFRLRDFSWTGEHSILDAKCLAEQNGGLYIGAQNK
                     DDLVDALKSTLECPMISSAWR"
     gene            16877..17587
                     /locus_tag="VFY74_09615"
     CDS             16877..17587
                     /locus_tag="VFY74_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5931989.1"
                     /translation="MVRSTATEARPSLAGITILAGLSPKALARLQESCTSRHYQAGES
                     IVDYLDASDDVFFVIAGEVRVTIYSLAGKAVSFRELGPGDVFGEYPAIDRGPRSASVE
                     ARTNCLVATLPGALFRKLIAEEPVVAQAMLPQLVMKIRALTTRVYEFSTLAVSNRIHA
                     ELLRLAKIGAKDGSGARISPAPTHTEIASRVSTHREAVTRELVRLSKIGLIERDKNVL
                     VIKDVKRLAAMVHEATGE"
     gene            complement(17598..>18052)
                     /locus_tag="VFY74_09620"
     CDS             complement(17598..>18052)
                     /locus_tag="VFY74_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931990.1"
                     /translation="SNVTAAFYGLTLANVGERFGWREADAISKAVFRRLGHLKTKEAL
                     ELGVDLPRDSRAPALVFITAVHSASPEYNFEVLEYLPDETTIRVFGISRYDRIAKSLD
                     IEHHLTWPELIPFFEGAAEELGIKCKVQGELKELGEQGRYECLYRCTL"
BASE COUNT         3113 a         5806 c         5810 g         3323 t
ORIGIN      
        1 gagccggccg ccgaaaccac gcggaccggt gtcagaacaa acgctgaacc taccgccaga
       61 gccccatatt tggcccagga cggccggaag catcccctgg ctctttttga tccgctgccg
      121 cggggagccc tgaagggcct acgctgcaac cttttatgca acctttgccg aactggcttg
      181 cccctgaatc cgttggtttt gttggtaccg ccgggtgaat tcgaatcacc gacctcttga
      241 tccacagtca agcgctctaa ccagctgagc tacggcggca tgctctagga ggattcgggg
      301 agaacctaag cggagcgccg agccgttgca agcgcgggcg ccgcaagtag ccatgcccgc
      361 tcgcgccgag ccgttgcaag cgcggggcgc ccggaaggac cgaaatcatt tcaagccaat
      421 aaagaggccc gggggctgac ccccgggcct ttagcgcgcg accccgtatc ggtcccgcgg
      481 atatcgtccg cggggcgaag gattcgtgcc gttacgactt gcggacctgc tcgaaagagc
      541 gggcgacctg gctcttgatc ggctcggcgg tatcggcggc gatcttggtg ctcagcgccc
      601 tgatctcgtt cgcctggctt gcgaacacgt cgaactgccg gcggatgaag gccgactgaa
      661 gctcgacgat ctcggcgacg ttcttggcgc cggcgaggct cttggcgagg tcgaaggcgg
      721 agttcagatt gcgctgggca aggtcgatga gcttgcggtt gaaggcggtg gcgccctgcc
      781 ccgctgcatc gaacgaccgc tcgagcgcat cgacggcctc ttccagcgcg tccttgccgc
      841 gctcataggc ctcacggctc tggtttacgg tcttctcggc gagagcgcga acgctctcag
      901 ggacagtcgc atcgagcttg aacgactcga attccttggc ggccttgcgg aacgtatcgc
      961 gggcggcccc ggcgacggct tggggggaat ttgcttggct catgatggcg cacctctgct
     1021 tggacgtgtt tcacttcagt cgggggtttc cggcttggcc ctttcgaaat ggctgccgcg
     1081 atctttgttg cactgcaata taattgttgc ggtgcgataa tcaagggctt ttcgcggcgc
     1141 gcccggcttt ttttttgcca tgaccgcaat ctgcccgggt ttcggcctag gcaaagcgct
     1201 gaattcgcct aaatttagcc gatgagtcga ggaagcaccc ccaggactgg gggtttgagc
     1261 gtggcttatt gaaaggcccc ttttcgccaa ggcgactgaa tgagctctct cccgttgcat
     1321 atgcccgacc tcaagccgaa caggcgcgcc gacgagccgg acaaggaccg cgaggctctg
     1381 gagcatattg cccgcgcgct cgagagccgt aggttcgagc ccccggccga aggcgcttcg
     1441 gtcggggagg ccgcggaggt cttgctcacg cagcagagcc tgaacagcct gctcgagagc
     1501 atgccggcgg cggccggcat cctcttcggc ggcacactcg cgcatgccaa ttccgccttc
     1561 gccaatgcct tcggctatcc gtcaaccgcc gagctcattg aagccggagg gcttcgcgcg
     1621 atcctcggcg aaggcaaggg ccttggcgag gtgaggtccg agccaagcgc gccggtcgag
     1681 gcgctgaccc gatcgcggcg gcgcgtgaag gtcgcctttg cgctgagcgt tctcgacgcc
     1741 gaacgggagt tgacgctgct gcgattgatc gaaccgccga tcctcgcggg cgaagcggag
     1801 gcgcaagctt caccggtggg ggtgccgccg aagcctgagc cggcgccgag gccccccgcc
     1861 gacgccgctt cgagccgcct cgactttctg gcgaaagtca gccacgaggt gcgcacgccg
     1921 ctcaactcga tcatcggctt cgccgagctg atgcttcagg agcgcttcgg tccgatcggc
     1981 agccggcgct acaaaggcta tgtcgaggat atccatcaga gcgggctcta tgcgctctcc
     2041 cttctcaacg acctcctcga catctccaag atcgaggccg gcaagttcga gctcaatttc
     2101 accgcggtcg acgtcgccga aatcgtcgag gattgcgcag cgagtcttca gccgctcgcc
     2161 aagcgcgcgc gcatcctgct caggacttcg ctcgcgccgg accttcccac cgtcgtcgcc
     2221 gacccgcgcc ggctcaagca gtgcctgctc aatcttctca ccaacgccat caagttcacc
     2281 aatgaaggcg gccaggtgat cgtgtcgggc tccatggtcg acggcgagct tcgcttgcgc
     2341 gtgcgcgaca acggcgtcgg catgagcaag gacgagatcg ccttcgccat gcaacccttc
     2401 caccagctcg acactgcgcc ccgcaagcag acgggaacgg gcttgggtct ccccgtcacc
     2461 aaggcgctgg tcgacgccaa ccgggcgcgg ctcgtgcttt cgagcgagcc gggtgtgggt
     2521 acatccgccg atgtgatctt cccggccgag cgcctcttca cttccggctg aaccgcggaa
     2581 gccgagccgc gtctctactt ggcgccggtg gatcagtcgc gcgctcatgg cagtcgttct
     2641 aaagggtttc tgacactatt acatctgcgt tactttccct gatctcaaga ttagaagatt
     2701 tctattcttg atattgaaat tagaaggctt ctaatgtaga taaggtcaag tcattcctgt
     2761 tgacattctc aaaaccgaaa ctttaatcga ggcagagagc ccgccgattc taaggacggg
     2821 cgctgatctc aggtccgatt ccggggcttt gaactccggc gattggaggg aaagatgcga
     2881 tttgcctcgg cgcgctttct ggcgctcgcc actctcctcg cattcgccgc gttgcaagcg
     2941 gacgccgccg accccggcga ggtgaacatc tattcctatc gccaacccta cctgatcgag
     3001 cccctgctga aggagttcac cgaagagacc ggcatcaagg tcaatgtgat cttcgccgag
     3061 aagggcctga tcgagcgcat cgaggccgaa ggacgcaaca gcccggccga tgtgctcctg
     3121 accgtcgata tcggcaatct cacgcaggca accgccgccg gcatcgcgca acccattcgc
     3181 gtggacgcga tcgacgccgc gatccctgag gcctatcgcg ccgaagacga tcagtggttc
     3241 gggctcaccc gccgcgcgcg cgtcatctac gcctcgaagg aacgggtgaa gcaggacagc
     3301 atcacctatg aggaactcgc cgatccgaaa tggcggggca agatctgcac tcgctcgggc
     3361 cagcatccct acaacgtcgc cctgatcgct tccataatcg cccatcacgg cgaagcctgg
     3421 accgaagact ggctgagagg ggtcaaggcc aatctcgcgc ggaagcccgc cggtgacgac
     3481 cggctccagg tcaagggcgt ctatgccggc gagtgcgaca tcgccatcgg caacacctac
     3541 tacatgggag cgatgctcaa gaacgataag cagcccgagc agaagcaatg ggccggctcg
     3601 gtcaatatcc tgttccccaa tacgggagat cgcggcacgc acgtcaatgt ttccggcgcg
     3661 gtcgtggcaa aacacgcgcc gcacaaggac aacgctgtga agctcgtcgc gttcttggcc
     3721 tccgacaagg gccagcaact ctatgcggag gcgaacaacg aatatccggt caaggaaggc
     3781 gtcccttggt ctccgctcgt caagtcgtgg ggtgagttca agccggaccc gatctcgctc
     3841 gacgaaattg cgcgcttacg caagacggca agcgagctcg tcgacaaggt cgaattcgac
     3901 gagggtccaa gctcatgagg gggcaacgat gagctttcaa aaaaaggtgg atcgccaggg
     3961 ttcggtcaga gtggcggcat gggatcgcat accgccgcga agcggggcgg agtggctcgt
     4021 cggcatgggg tttcgctgct ggcttgcggg ttacgacacc ggcgacatcg cctgctggga
     4081 gaacggatgg aacgaatatg cgcgcgcgct cggccccgag cgggcaaggc gggctgtgac
     4141 cgagcttgca tgctgggtcc gggccgtgcg cgcctcggcc tcgcgcaaga tcgagtattc
     4201 tcccgtcggc tgtccggggt tctgcgccga tgagtgcctt gcgatctcgc tgatcgcggc
     4261 ctctcagcat catcgctgtc cggcgatgcg ggcctgcgcg ctcgcgctga cgggttcgga
     4321 ttcggtcgat cccgtgctga aagccgcgaa cgccttcgcc gatgcgctcc aggaagccga
     4381 tcagcgcctg tcgccggaag ccgtggcgac gctgatggca ggacggcgca aaggcggcgc
     4441 agaccgtctc gcgctcggct gagcgcgggc cccgccggaa gacaggcgat ggctctggcg
     4501 ctgtcatccg agcggcctca atggcgccgc cggttggccc ggctcaagga tagcggcaat
     4561 ctcgggtggt cggcggcggc gctcctaatc tcgctgatcg ccctcctgcc catcctcgcc
     4621 atcggcggaa tcgcgctcaa gacgagcggc gacacctggc cgcatctcat cgagaacgtg
     4681 ctcccgggag cgcttcgccg caccgcgctt ctgctggcag gcgttggcgc attgacgctc
     4741 ctcatcggca ccgccacggc atggctcgtg accatgtatc gcttccccgg gcgaaacgcc
     4801 ttccagtggc ttctgctgct gccgctcgcg atccccacct acatcatcgc ctattcctat
     4861 ctcgagctgc tcgattattc cggcgtggtg cagagcggct tgcgcgccct gttcggctgg
     4921 caagacgcgc ggagctactg gttcccggat atccgtagcc ttggcggcgc gatcctcgtc
     4981 atgagcgcgg tgctctatcc ttatgtctac atcaccgcgc gggcgagttt catcgctcag
     5041 tcggtgtgca tcctggaggt gagccgcagt ctcggccgca cggcgcccca gaccttctgg
     5101 gaggtcgcgc ttccgctggc gagaccggca cttgccgctg gcgtcgcgct cgcgctcatg
     5161 gagacgctga acgatatcgg ggcggtcgga ttcttcgggg ttcgaacctt gacggtcgcc
     5221 atctacgaca cctggctcga tcgcaacaac ctcgccggcg cagcgcagat cgcctgcgtc
     5281 atgctgctct tcgtcttcgc gctgctcggc ctcgaactgg cgctcagagg caagcgccgc
     5341 tttcaccata ctaccggcaa ataccgccgc ttgccgaagg atcgcctcga gggcttgggc
     5401 ggtgcgctcg cgagcctcgc ctgcgccgtg ccggtcttgt tcggcttcgc gctgccggcg
     5461 ggcgtcctgt tctacgacgc actcgcccat tcggcatcgg gtctttcgcc ggcgttctgg
     5521 cgcgccgcct tgaacagcct cctgcttgcg ggtgcggccg ccgttcttgc ggtcggcttc
     5581 gcggtggtcc tggcctatgc acggcggcaa acccgctcga agctcattca gggcgcgagc
     5641 acgatccccg cgatcagcta cgccgtgccc ggcaccgtgc tcgcgatcgg cctcctgatc
     5701 ccgctcgcaa gtctcgacaa cttcgtcgac tctctcaccc gatccctgct cggcgtcacc
     5761 acgggattgc tgctgtcggg gactgctttc gtcatcgtgc tcgcctatac gattcgcttc
     5821 cttgccgcct cgctcggggc ggtcgaggcc ggcttgagcc gggtgtcccg caacatcgac
     5881 gccgcggcgc gctcgcttgg cgccacgctc ggcgaaatgc tcgtcaaggt gcacctgccc
     5941 ctgctcaggc ccgcgctcgg cgccgccgcg ctgcttgttt tcgtcgattc gatgaaggaa
     6001 ctgccggcga cgctcctgct cagacccttc aacttcgaca cgctcgccac gcaggtcttc
     6061 acgctcgtct cgctctaccg gtacgaggag gcgggattgt cggcgctgac gatcgtgctg
     6121 gtgagcctcg cgcccgttct cctcctccac gggctcgtgg cgggaagtcg gccgggagcc
     6181 tcgatcgcag ccggtcgcga ggggccgtga ttggccattt tcggccctcg cgatcccaca
     6241 acaaaaagcc ggcctggtct cgctatcccg atgcgttcag gctagaaagg cgggggtcga
     6301 aaaatcggga aaaaaacggc gaggatctcg gctttcgggt ccagcgccga aaacgggtgg
     6361 atagggacgg catgaagcct cgccagatcg cttggaaatg gctgcttctc ggcgtcttcg
     6421 tgctgtttct ggccggtctt gtcatcctgc cgcggctctt cggcgactct ttctctctcg
     6481 ccaatcgcgt gtcgggcgcg atcgcggcgt ggacgggggg agaggtcaag ttgaccgggc
     6541 ccttgaagct tcagtacttc cccgatgtgg cgatcaggag cgggttcgag ctgaccaggg
     6601 cctcgcgtct tccgttggtc gagtcgatat ccgcaaagga ggcccggatc tcgctcgatc
     6661 tcgccgcgct cttcctcggc cgcatccatg tcgatgccgt tcgtctcatc aggcccgaga
     6721 tcacgctgaa agaggcgcca tctctcgtca cgggatcgga gcgaacgttg caagcgcgtg
     6781 tcgccaacgt gctgacggac gcgccattcc gcgtgcttcg cgtgcgcgcc ggctccatca
     6841 ggatatcgac cccttcgggc ggcgagacga tcaagacgtt caacgcgcga ttcgagctcg
     6901 ctcccggcac tcgggcgctg tcgagttccg gttctttcgt gctgcgcaat gaaaccgtcg
     6961 cctttgcact ccattccgac atgccggcgg agactgcgga cggcctcact gtcccggtga
     7021 gactcgcctt caatgccccc acgattggcg ccacggccac cggcacggca tcattcacca
     7081 acagatttca cctcgacggc gatttgaaag ccgacatggc ggatgcccgc gcctttctca
     7141 gatgggccgg gatccccttg gccgagggag agagcctcaa gaatctctcg gcatcgggag
     7201 gagcgcagtg gaacggcaca acgcttatct tcgacgacgg cgccttcgcg ctggacggta
     7261 acgctgcggt cggcgtgctt gccgtcaccc ccggcaaacg cccgcgcatc gatgggacgc
     7321 ttgcgttcga ccggctggcg cttgatccct atctgacaag cagggccgca cgtccggatc
     7381 aatccgctct cagccatttc gacaccgacc tgcgcatctc ggcggcggac atcaccgccc
     7441 ctcaagtcaa gctcgggcgc ggcggattca ccatcagcgc gcgcgagggt ctcgtttcaa
     7501 ccgaggtggg cgagcttgaa gtctgcggcg gcacggtcac gggccgaatt gacgtcgatc
     7561 tttccaacgc catcaacaag tccacgctcg ccgggaaact gtccgatgtg ccgatcgagg
     7621 actgcctcgg tccgcttggg ttgcaggtcc ccctgagcgg cgtgggcagc ctgaaggttg
     7681 agttctccac cgagggaagc aactatgacg agctggcgcg agggctcggc ggcccattca
     7741 agctcagagc ccgcaacggc gtcgtttccg tcgatctcgc tcgtctcttt gcgggatcgg
     7801 gcccgatcga gggcgaaggt tggagcagcg acaatgttac ggcgttcgag gacctgatcg
     7861 ccgaatgccg cctcggtgcc ggccatattt ggtgcgagaa gttcaacatg aaaacagagc
     7921 gcggattgat ttccggatcc ggcgacgtcg atctagggca gaagacgctc gattggcgct
     7981 tgttcgtcgc cgatcacgcc cagcctttga aggcttcgca gctgagcgcc gaaaccccgc
     8041 cgcaaatctc gataagcggg tcgctcgcgc aaccggtgat ccggagggcc gacggtcggg
     8101 gcctcggcac cggatcggtg ccggcggcga ccaaggcgac gcaagtttcg ccgcgctgag
     8161 cttcctggag gacgtcattc aactgagcct gagaaaccca gtgtcccaac accgcacctt
     8221 ccgtttctgt cgcgcgctcg cgctggcggg catgaacgcc cgggcgtgcg cccttccgct
     8281 cgctttggcc gccggcctcg gcgcctcggc aagcgcgctt gccgaggaca cgctcaacat
     8341 cgcgacgtgg gggggcgcct acggtcaatc gcaggagatt gcctttttcg agccctatgc
     8401 gaaggaaacc ggcaccagga tcgcgaccga gatctatgac ggcacgctcg ccaagatcaa
     8461 agagattatc gagggcggct cctcggcaat cgacgtcgcc gacgtttcgg caggaacgct
     8521 caacgccctt tgcaatgacg ggctgctcga gacgatcgcg gcctcgagcc tcactgcggc
     8581 gccgtcggga gagagcgcgg aacaggactt cttcccgggc agcctttccg cttgcggtgt
     8641 tgccagcgtc gcctggtcta ccgcgatcgc cttcgaccgg caggccttca gcaaggcttc
     8701 gccctcagac atcgccaatc tcctcgacac gaagcgcttc cccggcaagc gggcgctgcc
     8761 caacagtgcg caccggacgc tcgagcttgc gctcctcgcc gacggggtcg atcccgccaa
     8821 tgtctatacc gaacttgcaa cacaagcggg cgccgaccgc gccttccagg cgctcgacaa
     8881 gatcaaggcc gagatctact ggtgggacag caccgaagag ccgatgacct ggctcttgga
     8941 aaaaaaggcg gcgatggcgg ctgcctatag cggccgcatg ttccgcgcca tggtgggcac
     9001 ccgccagaga ttcggcgtga tctttaacgg acaggtctac gatctcgatc tgtgggccat
     9061 ccccaagagt gcgaggaaca aggaaggcgc gttgcgcttc atcgcgttcg ccaccgaccc
     9121 gggccggctc gcggtacagg cgcaactgat cgcgtatggt ccgatgcgaa aatcggcgct
     9181 tgctctcgtc ggcaagcatc cggtggtcgg cgtcgagatg ttggcctttc tccccacggc
     9241 gccggacaac ttcaagaagg cgctcaaatt cgacgagggc tggtgggaca cgaacggcgc
     9301 ggcattgcag tcgcgctttg aggcatggcg tgggccggtt gctcccggac aaggcaagcc
     9361 cccgatcgtc caggccaagc cgcaagaggc ggaggcaagc cctcggcgcg caaaagcgct
     9421 tccgtgacct tcgcgtttgt ccgggctgtt gcgagcgcgg cccgacctcc caaaccctaa
     9481 cactccggag cgtgtccaag aggccgcagg tcaagtctca actgaagccc cgcagataaa
     9541 aagaagtgcg ggtgagattg aactcccgtt tgcattgatc gtcatcgcta tgtcattctc
     9601 tccgccggag agagtcggtt gggtgtcgac cccctgcgga catccctcct acacaggatg
     9661 cacgacgacg aggcgatcag cctcgaccga gtcgcttcag cgcaaagtcg gggtgtcgtc
     9721 ttgtgatgca aagcactgcc ggagcggggc cggcagtgcg atttcccctc gaaacccctg
     9781 gcaatccaac cttttacgac ctttatacag ggcaattcca cggcgcattc tgccgggcgg
     9841 tgcgcccgga gatcggcgtc gcctgcgaga agcttgaaca tgacacccga acgattcgag
     9901 agctggctta ccgtcgatcg gaagggcctc ttctgccgtc ccggagcctt ccatatcgac
     9961 ccttacggcg gtgccaagcg cgcggtcgtg acccacggcc attccgacca tgccaggccc
    10021 ggacacgatc acgtgcttgc cacgcgcgaa acgctcgccg tgatggcggc gcggctcggg
    10081 acggcggcgc gagcgagcag tcaggcggct tcctacggcg aacgcgtcac gatcggcgac
    10141 gtggcagtga cgctgtttcc ggccggccat gtgctcggca gcgcgcaggt cctgatcgaa
    10201 tggaaaggcg cccgggtcgt cgtctcaggc gattacaagc ggagcgccga tccgacctgc
    10261 cccggctttg aggtcgttcc atgcgatctc ttcatcaccg aggcgacctt cgcgttgccc
    10321 gtgttccaac atggcgaggc gcgcgaggag gttgcgaagc ttcttgcgtc gcgcgcccaa
    10381 tttcccgacc gcgcccatgt ggtcggcgtc tatgggctcg gcaaatgcca gcgcctgatc
    10441 gcgctcttgc gcgaagcggg gtacgaccgc ccgatctatc ttcatggcgc tcttgcagag
    10501 tgttgcgcgc tctacgagtc tttcggcgtg cgtctcggcg cgctgaaacc cgcaaccggc
    10561 gccccccgcg acgcgctcaa gggtgagatc gtgctggcgc ctccttcggc catcgccgat
    10621 cgctggtcgc gccggatgcc cgatcctgtg acggctttcg cctcgggatg gatgcgggtg
    10681 cgcgcgcggg cgcgccaacg cggcgtcgag ctccccctcg tcatctcgga tcatgccgat
    10741 tggcgcgagc tgaccgagac catcgccgac accggcgcgg gcgaagtctg ggtgacgcat
    10801 gggcgcgagg acgcgctcct gcatcacatc cagaagacgg gccgcaaggg ccgcgcgctt
    10861 gccctggtcg gccgcgagga cgaggaccag tgaaggcctt cgccgcgctt ctcgaccggc
    10921 tggcctatac gccgtcgcga aacgacaagc tgcgactgct ggccgactac ttcgcgagca
    10981 cgcccgatcc ggaccggggc tttgcgctcg ccgcgctgac ggacggcctg ttcttcaggc
    11041 tgcccttccg gcgcatcctg accgaactgc tccagccgcg cgtcgatccc gtgctgttcg
    11101 acttctcgcg cgactatgtg ggcgacacgg ccgagaccat cgccctgatc tggccgagcg
    11161 gccgcgccca cgccaagccg ccgcggctct ctgccgtcgt cgaggccttg cagtcggcgt
    11221 cgcccgccga ggtccctgcc ctccttgcca attggctcaa tcaactcgac tcgaccggcc
    11281 gctgggcctt gctcaagctc ctgaccggcg cgctccgcgt cggcgtttcg gcgcggcttg
    11341 ccaagacggc gcttgccgag tggtcgggcg tggcgcttgc cgacatcgag gaggtctggc
    11401 acggactgcg gccgccttat ggcgaattgt tcgcctggct ctcaggcaag ggcccgcggc
    11461 cggcgctcga aggccgcgcg acgttcaggc ctctgatgtt gtcgcatcag atcgacgaca
    11521 aggagcttca ggcgatcgcg cccaatgact tcatcgccga gtggaaatgg gacggcatcc
    11581 gcgtgcaggc cgcccgtcaa ggcggcgaga agcgtctctt ctcgcgaacc ggcgacgata
    11641 tctcgggcgc cttccccgat gtggtcgagg cccttcccga aggcgtggtg ctcgacggcg
    11701 aactgctcgt catgcgcgac ggcgaggtcg ccccgttcaa cgatctgcaa cagcggctca
    11761 accgcaaaat cgtcagcggc gccctgctca agtcgcatcc cgcgcatatc cgcctctacg
    11821 acattctgtt tgagggcggc gaggacctgc gtgcgctccc ctttgtcgca aggcgcgcgc
    11881 ggctcgagtc ctggcacaaa acgcatgctc cgcgccggac cgatctttcc accctcatcg
    11941 acttcacctc ggttcaggaa ctccagacgc tatggtcagg cgcgcgcgaa accgggatcg
    12001 aagggctgat gctgaagcgg cgcgacagcc cttacctcgc cgggcgcccc aaaggccatt
    12061 ggtacaaatg gaagcgcgcg ccgcttgtga tcgatgccgt gctcatgtac gcgcagaggg
    12121 gaagcggcaa gcgctcgtcc tactattcgg actacacgtt cggcgcctgg cgcgacggcg
    12181 aggcggggga ggaggagttg gtgccggtcg gcaagtccta tttcggcttc accgacgagg
    12241 agctgaaaga gctggatcgc tgggtgagga accacacggt tgacagcttc ggacccgttc
    12301 gccaggtcga accgaagctc gtgttcgagg tggccttcga ctcggtgcac cgttcgaccc
    12361 gccataaatc gggggttgcc atgcgcttcc cgcgcatcaa ccgcattcgc tgggacaagc
    12421 cggccgcgga agccgatcgg ctggagacgg ttcaggcgat gatcgtggac tgagccgatg
    12481 acccaagggg gatcatcgcg cgccgggtgg ggactttcct ttgacgcggg ctatcgcccg
    12541 ctgacccttc accgcgcgcc aaatggcgcg cgtgctcccc cacaagggga gagcggggcc
    12601 atgatatgat agcgaggtat agtcggtgga ctttgaaata caatgtggtt gatgagacgc
    12661 tttgcgatcc tcactttggt tgcggggatg ctcgtgcttg ccgcaggccc ggcgcaggct
    12721 caagacgtcg cgatctgctt ccgaacggca gaccgtgtgg tgagcgggga agatatcgat
    12781 gacgcggcca agaaggcggg gcacgaagcg tgccagcgcg cgctcgcgga gacctcgagc
    12841 gtcgtgcaga aataccattt gcaggaagcc gatttcgaca tcgtcggacg cccgcccaag
    12901 gcgccgaatt aagcacgggc gatgtttagc tcgcgcgcgc tgccgtcggc tgcacgatga
    12961 tggcgccgcc gtcgccgccg atgatctcct cgtcggcgag cttgaaattc tccatgcgca
    13021 cggtgtgata gcggtcgaaa tagtcgttga gcggcttctc gatcgccttg tcgtaatcgg
    13081 gccgcacaac gtgcgttgcg ccttgcacca ccttcaccac cttcccgttc ttgaccacca
    13141 cttcgccgtt cttgaacacg aactcgggtg tcgcgaacat cgcctcgcgg tcgggatcgt
    13201 cgcgatagac ggtgatatcc cctgacgcac ccacgccgag gtgcccacgg tccttcagcc
    13261 ccaggctccg cgccgggccg gcgcgtgtga gtatcgcgat ctcgtcgagc gtgtattcgc
    13321 gggtgagcga tttcagcaat tcgctctgcg cctgcgcgtc gggattgatc ttctgcagca
    13381 tgtcgtcgcg gaagcccttg tccatgagca ggcggatcag atgcggatag aagtagaagg
    13441 gcgcgccgtt cggatgatcg gtggtaagga agatacgcca cgggtcgtcg aaaagcagga
    13501 acgtctcgag tccgatcacc cattgcatgg cgttgacgaa gctcttgtcg cgatagcgaa
    13561 taggcacgac gccgcagccg gcatcgcatt cgatatccat gatcaccggc ttcttcgggt
    13621 tcgcgctctt ggagatggcg tattgacgca tgctgtcgcc ggacgcggtg caggtctgcc
    13681 cgaacaggac ctggccgacg tcgatggagg cgttcggagt tttgttgacg agctccgtga
    13741 ggcgggccgc gcccgaggag aattttcggt ccccctcgat gccgtagctc aggaactggg
    13801 tgtgcgtcat atggatgcgc aagccctcgg cggcccgcac cgtggcgagc gtcgtctcga
    13861 tattgccggg aacgccgaga ttgcagccat gaatatgcag cgggtgggtg acgccgagct
    13921 cctcgatggc gcgcgcgagc gtgaggagga tctggcgcgg cgtcacgtgg tagtagacat
    13981 gctcctcgtc gagatcgagc ttgcgctgat tgaacttgaa ggcgctgatg ccgcccgggt
    14041 tgacgacctt caccgcgatc gcctgggctg cgtgaagcgt ccaggcgaca tagtccttga
    14101 tcgccttgaa gtccttcttg gcggcgagct ggcgcaggaa gtaatcgtcg gagccgagca
    14161 tgacgaaggc gcccttatcg accatgggcg tgtcgcccat ttccatatgc gcctggcggg
    14221 cgttggcggg cagcatggcc ggctcgaaac aggcggtgta gcccatctcg gcgtagcggt
    14281 agccggtgac atgggtcgag ggcacggcac ggccggagcc cgcgcgggtg agatcggtgc
    14341 gggtgacggg atcgcccctg tggtcctcgg gcaggagcat gcgcgcgatg gtcatcttgc
    14401 cgccgccgat atgggtgtgg ggatcgatgg cgcctgccat gacgatccgg cctttgacgt
    14461 cgtaggtcgc atcagcacgc gcgttgggcc tggccgcgac gatcttgccg tcctcgacat
    14521 agatgtcgcg ggtctctccg ctcacattgt tggcggggtc gtaaaccttg ccgccggtca
    14581 gcttgatgag cattttggaa gctcccggcc taaagcgccg cttcgatggc cgaaagcatt
    14641 tcggctgcgc taggaagctc cgcgcggccg agatctttca gcggcaagga catgacgttg
    14701 tcgacacgaa tgagacgccc ggcatgatcg aggcccggcg tgccgatagg aacaaagacc
    14761 gcggggggtg cggcgagggc aagccccggc gtgccgagca cgatggtggg gatcttggtt
    14821 ggaggcggcg cgaggtcggg cgagatcgcg gcgacccata cgagaagatc gccctccccg
    14881 tccccgagca tgcggccgat gtcccagcgg taaggatcgt aatcgggcgc gccgctcgca
    14941 tagctcacac ggagcggata gccgctgatc caggtgctga cggacgccgc cgtgggcgcg
    15001 ccttcggcgc cgcccagcga aagtccggcg aagcgaaccg tgggattgag gtccttcacg
    15061 agcccggtga actgctcgac ggcgagctcg gcatggggaa agtcgagcga aggcggcgcc
    15121 cagaccacga cgccgtacgc cgccttgcgg cagcgctcgg caagcgcatc gatctcggcg
    15181 agcgtgaccc cgctctgttt tcggggcgcg accttgaagc cgcggagccg cgctcgcaag
    15241 agcgccacca catgccccgc gtcttcgtcg ttgcatccaa tcgtgacgac atcgccgatc
    15301 ttcgagcctt tcgcgccgga gcggtcgagc cccttgccga tgaacacgac ggtgcgcttc
    15361 ggcgcggtga cgccgaacat cgagtccggc ggagagatga tccgctcgaa gaagcgggga
    15421 tggagccggt gaatatcgct gccggcgatg atgatcaggt cggcgcggtt gcgcgtctcg
    15481 gtcagcgtcg tcgtgatcca gccgctcgtc tggaggatct tgagattccg ctcaccgacc
    15541 gcactcaagg catgatcgac gatgccgccc gcccgttcgg ccagcgccaa tacggcgcgc
    15601 atgccttcga catcggtggc aagcccgccg aagagaggga gcttggcttt gccgatgagc
    15661 ttcgccgctt ccttgaccgc cgcggcgagc gcgacgtcct tgcccttcac ggaagggttg
    15721 gccggcggca gtttgcgctc gaagcccgcg atcgactggc ttgaactgtt ctttgcgacc
    15781 ttgagcccgg tcttggttcg ttcgacgacc agatcgtcgc aaagcgtgcc gcagaacggg
    15841 ttgaccacgt tctcgaacag atacgagccg ggatgctcgg ctttggcctg gcctggactt
    15901 gaacgcggca tgcgggctat cccccaagag gcgggggaaa gaaagcatgg gacaggcgtc
    15961 aacacaactc gcgagaggcc agaaacgcga tcttttttgc gaaattccct atcgccaagc
    16021 cgaggagatc atcgggcatt cgagcgtcga cttgagcgcg tcgacgagat cgtccttgtt
    16081 ctgagcgccg atatagaggc cgccgttctg ctcagccagg cacttggcat ccaggatgct
    16141 gtgctcgccg gtccaggaga aatccctcaa gcggaaacct atgacgtgga cggtcagttg
    16201 cgccgcggcg gcgctgagtt ccttgccgag ttcgcagggc gaagcgccgc aggtctcttc
    16261 gccatcggtc aggacgacga taacgcccgg atgccggcga tagtcgagca cttcggcagc
    16321 ctgcgccacg gcccgtgtga ggggcgtctt gcccgcgggc gatatgccct cgagtttgcg
    16381 caggatcagc tccgacgcgt ttggcgtagg cgtgaggtcg agcttgacat tgcactgatg
    16441 cccgaggccc ggaccatagg tgatgagccc catgcgacgg acgcgcgaga cagcggggag
    16501 gatctcggcg agtgccgcgc gcacctcgtc gatgcgcggc ttcaaggtgg caataccctg
    16561 ctcgaggttg ccgcccatgg aacccgaggc gtcgaatacg atcatgacgt cttcgctgca
    16621 gggcgaggcc gggacttcgg cacgcgcggc gccgggaccg gtgagaatgc cggcaaggct
    16681 cgctgccgcg acgagcctcg aggcatgctt gagaattctc gggggcatag cgatcctcct
    16741 tgacggatgt cgatcgcagt atgggtccgg gcggtccttg gcgctgtgag atttcccaca
    16801 ccaggcggcc cgccccgtgc cagttcatgg cgctagtgct atgctccgca ggccacgctg
    16861 acaggagcac cccaaaatgg tgcgatcaac agccactgaa gcccgaccgt ctcttgccgg
    16921 gatcaccatc ttggcgggac tttcgccgaa ggctcttgcg cgccttcaag agtcctgcac
    16981 ctcgcgccat taccaggcgg gagagtcgat cgtcgattat ctcgatgcgt cggacgatgt
    17041 gttcttcgtc atagcgggcg aggtccgcgt caccatctat tcgctggccg gcaaggcggt
    17101 gagcttccga gagctcggcc ccggcgacgt cttcggcgag tatcccgcaa tcgacagagg
    17161 cccgcgctcg gcaagcgtcg aagcgcgcac gaactgcctt gtcgccaccc tgcctggcgc
    17221 cctgttccga aagctcatcg ccgaggaacc cgtggtggca caggccatgc tgccgcagct
    17281 ggtcatgaaa atcagagcgt tgaccacgcg cgtttatgag ttcagcacgc ttgccgtgag
    17341 caatcgcatt cacgccgaat tgctgagact ggcgaaaata ggggcgaaag acggcagcgg
    17401 cgcgcgcatc tcaccggcgc ccacccacac cgagatcgcg agccgggtca gcacgcatcg
    17461 cgaggcggtc acgcgcgagc tggtgcgcct gtcgaaaatc ggcctcatcg aacgggacaa
    17521 gaacgtcctt gtgatcaagg atgtgaagcg cctggcggcg atggttcacg aggctacggg
    17581 cgaatagaga aagccctcta cagggtgcac cgatagaggc attcgtaccg gccctgctcg
    17641 ccgagctcct tgagttcgcc ttggaccttg cacttgatcc ccagctcttc cgccgcccct
    17701 tcgaaaaagg gtatgagctc gggccaggtc aaatgatgtt cgatatcgag gctcttggcg
    17761 atgcggtcgt agcgggaaat tccgaagacc ctgatcgtgg tctcatccgg gagatattcg
    17821 aggacctcga aattgtactc gggcgacgcc gagtgcaccg ccgtgatgaa cacgagcgca
    17881 ggcgcgcgcg aatcccgcgg cagatcgacc cccaactcca gcgcttcctt cgtcttcaga
    17941 tgcccgagcc ggcggaacac ggccttggag atggcgtcag cctcccgcca gccgaatcgc
    18001 tcgcccacat ttgcgagcgt cagtccgtaa aaagcggcag tgacgttcga ga
//
LOCUS       DASUQP010000214        16378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1331786, whole genome shotgun sequence.
ACCESSION   DASUQP010000214 DASUQP010000000
VERSION     DASUQP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 16378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16378
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331786"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFY74_09625"
     CDS             complement(<1..181)
                     /locus_tag="VFY74_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5931991.1"
                     /translation="MNFQEPLSGILERIAKALERLGPGKPARPDFDAAEAFVWQPETE
                     SFQPISDVNRVPLSLL"
     gene            312..671
                     /gene="yajC"
                     /locus_tag="VFY74_09630"
     CDS             312..671
                     /gene="yajC"
                     /locus_tag="VFY74_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5931992.1"
                     /translation="MPGFDYAQAAPGAFEMLNSLIIPTVLIIGIMYFLMIRPQQRRLK
                     EHRDMVAAIRRGDTVVTSGGIIGKVTKVEENELQVEIAEGVKIKIVRGTVSEVRGKGD
                     KSDKGEAAPASKAKAAS"
     gene            732..2348
                     /gene="secD"
                     /locus_tag="VFY74_09635"
     CDS             732..2348
                     /gene="secD"
                     /locus_tag="VFY74_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX5931993.1"
                     /translation="MLHFPRWKLILVLVVTVAAFIYALPNLFPAATMARMPEWLPHKQ
                     VNLGLDLQGGAHLLYQLDEKDMIEDQLGGIRGDVRETLRRERIGYSDLAQDVAKRSVS
                     VTIRDPADVDKAESELRKLAAPVGGNVFGGASANDMQVAREGSNRITLTVTDAGLANR
                     ITSAVEASIETIRRRVDALGTTEPSIQREGRNRVLVQVPGISDVERLKSLVGETGKLE
                     FKLVDPAADAQQAAVSKQVPPGDELVYSDETPPVPYVLKSQTLLTGENLENATPGFDA
                     QTNEPVVNFKFDAAGAKRFGRVTQENVGLPFAILLDEKVITAPVIREPILGGTGQISG
                     SFTVERANDLAVLLRSGALPAKLTVIEERTVGASLGADSVESGKKAALMGLALVMIFM
                     LAGYGLFGLFANVALILNVALIFAVLSLMGATLTLPGIAGIVLVIGIAVDANVLINER
                     IREEIRAGKSPYAAVDAGYSRALITIIDSNVTTLIAVLVLFWLGSGPVRGFAVTLTIG
                     ILASMFTAVTVTRLMVSYWLRWARPQYIPL"
     gene            2359..3312
                     /gene="secF"
                     /locus_tag="VFY74_09640"
     CDS             2359..3312
                     /gene="secF"
                     /locus_tag="VFY74_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX5931994.1"
                     /translation="MFRGIHFVPPNTKIDFVGLRMISWLVSALLTVVPLVLVATIGLN
                     MGIDFQGGTLIEVKTKESPANLADIRGKVSGLGLGEVQIQEFGAPDAVLIRIASQPTE
                     QDQQASIAKVKGALGDTVEYRRVEIVGPTISGELIASGTIAVVVALIGILIYVWFRFE
                     WQFAVAAIASLIHDVTGTIGLYSLFQLEFNVSSIAALLTIIGYSLNDKVVIFDRIREN
                     LRKFKRMPMVELLDVSINETLARAVLTHVTTFLAMLPFLFFGGETIFGFALAMCWGIV
                     IGAYSSIFVASPLQLILGVRREAWSTAPFSAKGRGPAAARA"
     gene            3334..4179
                     /locus_tag="VFY74_09645"
     CDS             3334..4179
                     /locus_tag="VFY74_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX5931995.1"
                     /translation="MHEGERGDTVRRIARSGDPDRALAASFAPRASRPDLLALCAFNV
                     EIARVAEQAREPELGAIRLQWWRDAIARSRQGEVSGHPVADAFGETVRRRALSTKRIA
                     ALIDVRQFDVATKIMPDWGALETYLKETAGGMFALGGACLGTQGPELGRAADQAGLAY
                     GLTGLMRALPVHAAGGRVFLPADRLVSHGTSPEALLAGSSSQGLRDLLAELRVKARAA
                     CEEARAHVARLGRSASAAFLPLRLVDPYLAALEALDRSGGDPLREIAAINPLYRFWRL
                     ANWRP"
     gene            complement(4314..5717)
                     /gene="trmFO"
                     /locus_tag="VFY74_09650"
     CDS             complement(4314..5717)
                     /gene="trmFO"
                     /locus_tag="VFY74_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX5931996.1"
                     /translation="MTGTVHVIGGGLAGSEAAWQLAQSGVPVALHEMRPKRQTEAHKT
                     GSLAELVCSNSFRSDEWETNAVGLLHEEMRRASSLIMAAADANKLPAGGALAVDRDGF
                     AAHVTRVLETHPLIAMSREEVGGLPPPDWDSVIVATGPLTSQALADAIKTLTGEAALA
                     FFDAIAPIVHAESIDMTVCWRQSRYDKAGPGGGGADYINCPLDKAQYEAFLAALLAAE
                     KTAFKEFEATPYFEGCLPIEVMAERGPHTLRYGPMKPVGLTNPHRPGGKPYAVVQLRQ
                     DNALGSLWNMVGFQTKLKHGEQLRVFRMIPGLEQAEFARLGGLHRNTFLNSPKLLDTG
                     LRLKADPRLRFAGQITGVEGYVESAAIGLIAGRFAGAERRGRALAPPPPTTAFGALLN
                     HITGGHLSAEDETGARSFQPMNVNFGLFPPIAGPAEPRAKGPRNTKSFARKRALSLRA
                     LADIERWLAHPASVAAE"
     gene            5937..6473
                     /locus_tag="VFY74_09655"
     CDS             5937..6473
                     /locus_tag="VFY74_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HEX5931997.1"
                     /translation="MSANTALAVSVGVLGAIATWLFLGPLGGALAIWAAFIAWGCYFH
                     CGGKEQGLQSAMVNNFAGALIAGLTLIVATRTGMAEMMGMPVWAAICVGFGVAAMVLL
                     ANVPLFATIPAQVYGFASVVAYTMMKDAYGYLTAVSLENPVVVVVLSMAIGAVFGYVS
                     ERLAGMLAGEPGARHAKA"
     gene            complement(6576..7064)
                     /locus_tag="VFY74_09660"
     CDS             complement(6576..7064)
                     /locus_tag="VFY74_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931998.1"
                     /translation="MDDWDGFYTLAGSTAGTLIGLIFVVITLGIQHTQGGDEHRLRLF
                     ITPVLVYFTSLLVIAMVMVPPLSAAARAIALGAIGSAGLAYVMNLFLLSRRTLKFEGP
                     RIIWHVVVPLVSYMLITASAAAWALEAPFANALCALGVTILLVTALRNTWMATLGIVK
                     RD"
     gene            7257..8072
                     /locus_tag="VFY74_09665"
     CDS             7257..8072
                     /locus_tag="VFY74_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5931999.1"
                     /translation="MPSVSVVWKGNCHDADTRYRLLGHLHRLASRSDEFLRLRQPRRP
                     HVLNVMNEQRGGAERLRPNIETIDEQASGTILVSGFVSPQPEQLIANARAAGVRIVEN
                     PEGQNAPLIALKRARLRGLDFKLFDPRGLYPGADRMSFVFLECEEHPFLDGWLVEIAN
                     AEDCAASGAEALRDADFYLCAPSVHLRYYLEDWTDCLFSWIKFFFIGDFWWHRWEEMQ
                     GFADYRQVFEDLQADRGVELAEEATFDAVLATFAQHAEHWIGEVEGWAKAEQG"
     gene            complement(8143..8787)
                     /locus_tag="VFY74_09670"
     CDS             complement(8143..8787)
                     /locus_tag="VFY74_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HEX5932000.1"
                     /translation="MARKSTAKTSTRKRAPRKRELVTAANVAGVGDIETFTDIRKLYD
                     RLAKGGKSMQAPDRSRLRLAAASVAMETAAESEAPWFAVARGELGQRDYPGPRHNPRV
                     VEYFNTTSLQANNDETSWCSAFVNWCMLQARQRGTNNAAARSWLAYGQALDNPRPGCI
                     VVLWRQSPSSWKGHVGFFDGFDVGNRIRLLGGNQGGGVEWDEVCLALFPQERVL"
     gene            9034..9648
                     /locus_tag="VFY74_09675"
     CDS             9034..9648
                     /locus_tag="VFY74_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5932001.1"
                     /translation="MAKLTLYHASPSRSSIVRWMLEELGEPYDLHVLRLANGEQHAPD
                     YLAVNPMGKVPALRHGETIVTEAAAICTYLADEFPQAGLNVPIGDARRGAYLKWLFFA
                     SCIEAAVTDRAFPRRDEPPRGMLGYGDFDTVMGVAAKAVEPGPYLLGEQFTAADVVLG
                     STLRWGMMFNLLPKRPQFVAYVGRLEQRPALQRATALDQELANA"
     gene            complement(9645..9923)
                     /locus_tag="VFY74_09680"
     CDS             complement(9645..9923)
                     /locus_tag="VFY74_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEX5932002.1"
                     /translation="MAKSGKKWSADLTAHDHPFDLEPGLFANGSAQQIAAALKKSAED
                     PARHSGDPYRTAMSMLDFYVNRAGAKLSVEQRAKLEQTKTALRQAFGR"
     gene            10034..10501
                     /locus_tag="VFY74_09685"
     CDS             10034..10501
                     /locus_tag="VFY74_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HEX5932003.1"
                     /translation="MKILSGGQTGVDRAALDTAIEKGLRYGGWCPKGGWAEDLPEPPG
                     LLALYPALRETPLAEPAQRTERNVRDADRLMILVDSAGISVSKGTEAARDLASRLGKP
                     HVVLDLDAGSVQVRAHTFLGEGKDGFSLCIAGPRESEAPGIYAKARAFLREVL"
     gene            10874..11473
                     /locus_tag="VFY74_09690"
     CDS             10874..11473
                     /locus_tag="VFY74_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate dioxygenase"
                     /protein_id="HEX5932004.1"
                     /translation="MNQDRPKLSVTRRQVLAGAATAASTVIGLRAGTAQTNPTGSTRP
                     NGKVCILTPEAVEGPFYFDPKLVRAAVAEGKVGAPLTVALQVVDAETCAAFNEARVDI
                     WHCDGLGVYSGYARQETGSAKGETFLRGTQFAASDGAVRFDTIYPGWYPGRTPHIHFK
                     VILDDKDLVTDQLYFPIPSESTSTQRILRTASASSSVTP"
     gene            11470..11595
                     /locus_tag="VFY74_09695"
     CDS             11470..11595
                     /locus_tag="VFY74_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932005.1"
                     /translation="MNANDFIFLEQRGADTLADTEEAGGSYRASLVIGINRQGRA"
     gene            11739..12002
                     /locus_tag="VFY74_09700"
     CDS             11739..12002
                     /locus_tag="VFY74_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5932006.1"
                     /translation="MLEALIIACVVGGIAGWLAGLLVQGTGFGLVGDIVIGILGALIA
                     AFLFPALGIALTVGGGIIGAIILAFIGAVILLVIVKVIKGMTA"
     gene            complement(12388..12675)
                     /locus_tag="VFY74_09705"
     CDS             complement(12388..12675)
                     /locus_tag="VFY74_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5932007.1"
                     /translation="MSKSELIQKVADECSMAKKDVKAVMEGLAGVAYKELRKSGTFML
                     PGFAKFIVVKKPATKERQGINPFTKEPTDFKAKPARKMLRVRPVKAAKDAV"
     gene            complement(13154..13399)
                     /locus_tag="VFY74_09710"
     CDS             complement(13154..13399)
                     /locus_tag="VFY74_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932008.1"
                     /translation="MRAEDFRENTIRKRKSVELVRDANYVAEVPVELIEDETGWSPYL
                     SVEDARKLDAVRLALRRGDVDAAAKYGRVFELTRVAG"
     gene            complement(14317..>16378)
                     /gene="uvrA"
                     /locus_tag="VFY74_09715"
     CDS             complement(14317..>16378)
                     /gene="uvrA"
                     /locus_tag="VFY74_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5932009.1"
                     /translation="YGACPACDGLGTELFFEPDLVVPDEDLSLNRGAIAPWARTSATS
                     PYYQQTLESLARAYRQPMTKPWKSLPKAFRDIVLYGSGDDEITFSYDDGVRHYSTTKP
                     FEGVITNIERRWRETESSWVRDELSRFQSNHPCQVCGGYRLKPQALAVKIARLHIGQV
                     TEMSIKEAACWFGDLPVHLTPKQNEIAARILKEIRERLTFLNDVGLDYLTLSRASGSL
                     SGGESQRIRLASQIGSGLTGVLYVLDEPSIGLHQRDNARLLDTLKSLRDMGNSVLVVE
                     HDEEAILTADHVVDMGPGAGIHGGEVVAEGTPDEVMAAPASLTGQYLTGFKQIPSPKA
                     RRKPTRGRRLSVLGARANNLKDITVDIPLGLFTCITGVSGGGKSTFLIETLYRAVARR
                     LSGAREVPGEHDAIEGLEHLDKVIDIDQSPIGRTPRSNPATYTGAFTPIREWFAELPE
                     AKTRGYKPGRFSFNVKGGRCEACQGDGVIKIEMHFLPDIYVTCDVCKGKRYDRETLEI
                     KFKGKSIADVLDMTVEEAAELFKAVPAVRDKLETLKRVGLSYIHVGQQATTLSGGEAQ
                     RVKLSRELSRRATGRTLYILDEPTTGLHFHDVAKLLEVLHELVDQGNTVVVIEHNLEV
                     IKTADWIVDLGPEGGDAGGEVVAVGTPEEIAANPASYTGQYLKQVMDRRRKAAGGKRA
                     AAAE"
BASE COUNT         2867 a         5212 c         5163 g         3136 t
ORIGIN      
        1 tgagcagcga cagcggcacg cggttgacgt cgcttatggg ttgaaagctc tcggtttcag
       61 gctgccagac aaaggcctcc gcggcatcga aatcggggcg ggcgggcttg cccggtccga
      121 gacgctcaag cgccttggca atccgctcca aaatgccgga gaggggttcc tgaaagttca
      181 tgtcgcgccg tttgcaaagc ctagcggggc ggctatagtc gcgccagctt cgaccggccc
      241 gcagcaagac gcaaggcgag ccgcgccaat cgaaggcggc aattccttaa gccaagcaaa
      301 ggacaggagc aatgccaggt ttcgactacg ctcaagccgc ccccggcgcc ttcgaaatgc
      361 tgaacagcct gatcatcccg acggtgctga tcatcggcat catgtatttc ctgatgatcc
      421 ggccgcagca gcggcggctg aaggagcatc gcgacatggt ggcggcaatc cgccgcggcg
      481 ataccgtggt gacctccggc ggcatcatcg gcaaagtgac caaggtcgag gagaacgagc
      541 ttcaagtcga gatcgccgaa ggcgtcaaga tcaagatcgt gcgcggcacg gtctcggagg
      601 tgcgcggcaa gggcgacaag agcgacaagg gcgaggcggc tccggcatcg aaagcgaagg
      661 ctgcatcctg atgccgtcgt cgacgttcaa ccggttcatc cgctagtccg atcgggagcg
      721 cgagcgcggt tatgctgcat ttcccaagat ggaaactgat cctggtcctt gtcgtcaccg
      781 tcgccgcatt catctatgcg cttcccaact tgtttccggc cgcgacgatg gcgcggatgc
      841 ccgaatggct tcctcacaag caggtcaatc tcggtctcga cctgcagggc ggcgcccatc
      901 tcctctatca gctcgacgaa aaggatatga tcgaggatca gctcggcggc attcgcggcg
      961 acgttcgcga gacgctgcgg cgcgagcgca tcggctattc cgacctcgcc caggacgtcg
     1021 ccaagcgctc cgtgtcggtg acgatccgtg accccgccga cgtggacaag gccgagagcg
     1081 agctgcggaa gctcgccgcc cccgtgggtg gcaacgtgtt cgggggcgct tccgccaacg
     1141 acatgcaggt ggcgcgcgag gggagcaacc gcatcaccct gaccgtcacc gacgccggcc
     1201 tcgccaaccg catcacctcg gccgtcgaag cttcgatcga gacgatcagg cgccgcgtcg
     1261 acgccctcgg taccaccgag cccagcatcc agcgcgaagg ccgcaaccgc gttctcgtcc
     1321 aggtgcccgg gatctcggac gtcgagcgcc tcaagagtct ggttggcgaa acgggcaagc
     1381 tcgagttcaa gctggtcgat cctgccgcgg atgcgcagca agccgccgtc agcaagcagg
     1441 tgccgccggg cgacgagctg gtctattccg acgaaacgcc tcccgttccc tatgtgctca
     1501 aaagccaaac cttgctgacg ggcgaaaatc tggagaacgc cacgcccggc ttcgacgcgc
     1561 aaacgaacga gcccgtggtc aacttcaagt ttgacgcggc cggcgccaag cgcttcggca
     1621 gggtgaccca ggagaacgtc ggtctcccct tcgccatcct cctcgacgag aaggtgataa
     1681 cggcgccggt tatccgtgag ccgatcctcg gcgggaccgg gcaaatcagc ggcagtttca
     1741 ccgtggagcg cgcgaacgat ctcgccgtgc tcctgcgctc gggcgcgctc cccgccaaac
     1801 tcaccgtcat cgaggagcgg acggttggcg caagcctcgg cgccgactcg gtcgagtcgg
     1861 gcaagaaggc ggcgctgatg ggactcgcgc tggtgatgat cttcatgctc gcgggctacg
     1921 gcctgttcgg cctcttcgcc aatgtcgcgc tcattctcaa cgtcgcgctg atcttcgccg
     1981 tgttgtctct catgggagcg actctcacgc tgccgggcat cgccggcatc gtgctggtca
     2041 tcggcatcgc cgtggacgcg aatgtgctga tcaatgagcg catccgcgag gagatcaggg
     2101 cgggaaaatc gccttatgcc gccgtcgatg ccggctattc gcgcgcgctc attaccatca
     2161 tcgattccaa cgtgaccacg ctgatcgcgg tgttggtcct gttctggctg ggctcaggcc
     2221 cggtgcgcgg gttcgccgtg acgctcacca tcggcatcct cgcgtcgatg ttcaccgccg
     2281 tgaccgtcac gcggctcatg gtctcctatt ggctgcgctg ggcgcggccg caatacatac
     2341 cgctctagga aacagccaat gttccgcggc atccatttcg tccctcccaa taccaagatc
     2401 gacttcgtcg gtctgcggat gatctcttgg ctcgtctcgg cgcttctgac cgtggttccg
     2461 ctcgttcttg tcgcaaccat cgggttgaac atgggcatcg atttccaggg cgggacgctg
     2521 atcgaggtca agaccaagga aagccccgcc aatctcgccg atattcgcgg gaaggtgagc
     2581 ggccttggtc tcggcgaggt tcaaattcag gaattcggcg ctcccgatgc ggtcctcatc
     2641 cgtatcgcct cgcagccgac cgaacaggat cagcaggcct cgatcgccaa ggtgaagggc
     2701 gcgctcggcg acacggtcga gtatcgccgc gtcgaaatcg tcgggccgac gatttccggc
     2761 gagctgatcg ccagcggaac gatcgccgtg gtcgtggccc tgatcggtat cttgatctac
     2821 gtctggttcc gcttcgaatg gcagttcgcg gtggccgcga tcgcttccct gatccacgac
     2881 gtgaccggca ccatcggtct ttattcgctg ttccagcttg aattcaacgt ctcgagcatt
     2941 gccgctcttc tcaccatcat cggctattcg ctcaacgaca aggtcgtgat cttcgatcgg
     3001 atcagggaga atctgcgcaa gttcaagcga atgccgatgg tcgagctcct cgacgtctcg
     3061 atcaacgaga ctctggcgcg cgccgtcctc acccatgtca ccaccttcct cgccatgctg
     3121 ccgttcctgt tcttcggcgg cgagacgatc ttcggctttg cgctcgccat gtgctggggc
     3181 atcgtcatcg gcgcctattc gtcgatcttc gtggcctcgc cgctgcagct cattctcggt
     3241 gtgcggcgcg aggcgtggag cacggcgccg ttctcggcga aggggagggg gccggccgcg
     3301 gcgcgcgcct gatcggaatg aggcgagcgg accttgcatg aaggcgagcg gggcgacacg
     3361 gtaaggagaa tcgcgcggtc aggcgacccg gaccgcgcgc ttgctgcttc cttcgcgccg
     3421 cgcgcttcaa gacccgatct tcttgccctg tgcgccttca acgtcgagat cgcccgcgtc
     3481 gccgagcagg cgagagaacc ggagcttggc gccatccgtc tgcagtggtg gcgcgatgcg
     3541 atcgcgcgca gccggcaggg ggaggtcagc ggccaccccg tggctgacgc cttcggtgag
     3601 actgtgcggc gccgcgctct gtccacgaag cggatcgccg cgctaatcga tgtgcgccaa
     3661 ttcgacgtgg cgacgaagat catgcccgat tggggcgcgc tcgaaacgta tctgaaggaa
     3721 acggccggcg gtatgtttgc tcttggtggc gcctgcctcg gcacgcaggg tcccgaactc
     3781 ggccgcgcag cggatcaggc cggcttggcc tatgggctga ccggtctgat gcgggccttg
     3841 ccggtccatg ccgcaggagg ccgcgtcttt cttcccgccg ataggctcgt ttcccatggc
     3901 acctcgcccg aagcgttgct cgccggatcg tcaagccaag gcctgcgcga cctgttggca
     3961 gagctcagag ttaaggcacg ggccgcctgc gaagaggcgc gcgctcacgt ggcccggctc
     4021 ggccggtctg ccagcgccgc ctttctgccg ctgaggctgg tcgaccctta tctcgcggcg
     4081 ctcgaggcgc tggatcgaag cggcggggat cccttgaggg agatcgcggc catcaatccg
     4141 ctctaccggt tctggcgctt ggcgaactgg cggccctgac acggttggag cctgctgcct
     4201 tagcgtgact tacgccgctt tcccgtcata ccggcgaaag ccggtatcca gagccatttg
     4261 ctcttgtgta tcccgctgga ttccggcttt cgccggaatg acggggacgc aggctattcc
     4321 gccgcgacgg atgcggggtg cgcgagccag cgctcgatgt cggcaagcgc acgcagagaa
     4381 agcgcgcgct tcctggcgaa gctcttggtg ttgcgcggac ccttcgcgcg cggctcggcc
     4441 gggcccgcga tcggcgggaa cagcccgaaa ttgacattca tcggctggaa gctgcgggcg
     4501 ccggtttcgt cctcggcgct gagatggccg cccgtgatat ggttgagaag cgcgccgaac
     4561 gcggttgtcg gcggaggcgg cgcgagcgcc cggccgcgcc gttccgcccc ggcgaagcga
     4621 ccggcgatga gcccgatcgc cgccgattcg acatagccct ccacgcccgt gatctgtccg
     4681 gcaaagcgga gccggggatc ggccttgagc cgcaagccgg tatcgagaag cttcgggctg
     4741 ttgaggaacg tgttgcggtg aagccccccg agcctcgcga actcggcttg ctcaagacca
     4801 gggatcatgc ggaacacgcg aagctgctcc ccatgtttca gcttggtctg gaagccgacc
     4861 atgttccata ggcttccgag cgcgttgtcc tggcggagct gcaccacggc gtagggtttg
     4921 ccgcccggcc ggtgagggtt cgtgaggccg accggcttca tcggcccgta gcgcagcgtg
     4981 tgcgggcctc gctccgccat cacctcaatg ggaaggcagc cctcgaaata gggcgtcgct
     5041 tcgaactcct tgaaggcggt cttttcggcg gcgaggagcg ccgcaagaaa ggcctcgtac
     5101 tgcgccttgt cgagcgggca gttgatgtag tccgcgccgc cgccgccagg tcccgctttg
     5161 tcatagcgcg attggcgcca gcacaccgtc atgtcgatcg actcggcatg gacgatgggc
     5221 gcgatcgcgt cgaagaaggc gagcgccgct tcgccggtga gagtcttgat ggcatccgcc
     5281 agcgcctgag aggtgagggg gccggtcgca acgatgacac tgtcccagtc gggcggcggc
     5341 aagccgccga cctcctcgcg tgacatcgcg atcagcggat gagtttcaag cacccgcgtg
     5401 acgtgggcgg cgaacccgtc gcggtcgacc gcgagcgcgc cgcccgccgg cagcttgttc
     5461 gcgtcggccg ccgccatgat cagagagctg gcgcggcgca tctcctcatg aaggagcccg
     5521 acggcattgg tctcccactc gtccgaacgg aaggaatttg agcaaacgag ctcggcgagc
     5581 gagccggtct tgtgcgcctc ggtttgacgc ttcggccgca tctcgtgcag cgcgaccggc
     5641 acgccgcttt gcgcgagctg ccaggccgcc tcgcttccag ccaagccgcc gccgatgaca
     5701 tgaaccgtgc ccgtcatgag ccatagctac gacccttccc gtccttcatc aagccaacgc
     5761 cctaagaccg gcgaagaacg aatcgcccaa aagcaggaat ccttgttgaa aaggccgagc
     5821 cttccactac gattctcgcg ggagccgaat cacctgcgta acggttccgc ccattgagcc
     5881 ggtggaccgc cggcaatccg gcgcatccgc ccgctcttgc acgaaagggg ggtgcgatgt
     5941 cagcgaatac agctcttgca gtcagtgtag gcgtgcttgg cgcgattgcc acgtggctct
     6001 ttctggggcc gctcggtggc gcgcttgcga tctgggccgc gttcatagcc tggggctgct
     6061 atttccactg cggcggcaag gagcagggcc tgcaatcggc gatggtcaac aatttcgccg
     6121 gcgcgctcat tgccggcctt acgctgatcg tcgccaccag gaccggcatg gccgaaatga
     6181 tggggatgcc ggtttgggcc gcgatctgcg tcggcttcgg cgtcgccgcc atggtgctgc
     6241 ttgccaacgt tccgttgttc gccacgatcc cggcgcaggt ctatggcttc gcctcggtcg
     6301 tcgcctacac gatgatgaag gatgcctacg gatacctgac ggcggtttcg ctcgagaatc
     6361 ccgtggtcgt cgtcgtcctg tcgatggcaa ttggcgcggt gttcggctac gtgtccgaga
     6421 ggctcgccgg catgctggcc ggagagcccg gcgcccggca cgcgaaggcg taaagcggca
     6481 ccggatctcg gcgttggggt gacccggacc ccggcgccca cacaatgcta agaggcgcag
     6541 tctccgcttg cggaggccgc gcctcttttg tcttcctaat cgcgtttgac aatcccgagg
     6601 gtcgccatcc acgtgttgcg aagtgcggtc acgagcagga tcgtcacccc gagagcgcag
     6661 agagcattgg cgaagggcgc ttcaagcgcc caggcggcgg ccgatgccgt gatgagcatg
     6721 taggagacga gcggcaccac gacgtgccag atgatccgtg gcccttcgaa tttgagcgtc
     6781 cggcgcgaca gcagaaacag gttcataacg taggcgagac ccgctgagcc gattgcgccg
     6841 agcgcgatcg cgcgcgccgc ggccgaaagc ggcggaacca tcaccatcgc aatgacgaga
     6901 aggctggtga aatagacgag aaccggcgtg atgaagaggc gcaagcggtg ttcgtcgcct
     6961 ccctgggtat gctggatgcc gagcgtgata accacaaaaa tgaggccgat caaggtgccg
     7021 gcggtgcttc cggcgagcgt gtagaaacca tcccagtcgt ccattccccg ctccattcgc
     7081 gccttctttc gcaatgacta taacttagcg ttaagcgaag gcgaaaagcg caggcccata
     7141 ggcagtcatc cgtgaacctc aaggcaaccc ttttccggaa catggcttgc gccatcgcct
     7201 cggcacgggc aatgtgcctc ccctcggttc gttagcgtcg tggcaggact tggcccatgc
     7261 cctcggtctc cgtggtctgg aaaggcaact gccacgatgc ggacacgcgc taccgtctcc
     7321 tcggccattt gcatcggctt gcgtcccgca gcgacgagtt cttgcgcttg cgacagccga
     7381 gacggccgca cgtgctaaac gtgatgaacg agcaacgcgg cggggctgag cggctccgcc
     7441 ccaatatcga gacgatcgac gaacaggcct ccggcacgat cctcgtctcg ggctttgtca
     7501 gcccgcagcc cgagcagctc attgccaacg cccgcgccgc cggcgtgagg attgtcgaaa
     7561 atcccgaagg ccagaatgct cccttgattg cgctgaagag ggcgcggctt cgcggtctcg
     7621 acttcaagct gttcgatccc cgcggactct atcccggcgc cgatcgcatg agcttcgtct
     7681 tcctcgaatg cgaggagcat ccctttctcg acggatggtt ggtcgaaatc gccaacgccg
     7741 aggattgtgc cgcaagtggc gccgaagcgc tccgcgatgc ggatttttat ctttgcgccc
     7801 ccagcgtgca tctccgctac tacctcgaag actggaccga ctgcctgttc tcgtggatca
     7861 agttcttctt cattggcgat ttctggtggc accgctggga ggaaatgcag ggcttcgccg
     7921 attaccgcca ggtattcgag gacttgcagg ccgaccgcgg cgtcgagctc gccgaggagg
     7981 cgaccttcga tgcggtgctg gcgaccttcg cccaacacgc cgaacactgg atcggcgagg
     8041 tcgagggttg ggccaaggcc gagcagggct gaccgcgcaa ctgtacggag aagcccagta
     8101 ctcgatctgg gtaattttct agcggccgat cggccaccga tatcacagca cgcgttcttg
     8161 cggaaacagc gctagacaga cctcatccca ttcgacgccg ccaccttgat tgcccccaag
     8221 caaccgaatg cgattcccga catcgaaacc gtcgaaaaag ccgacatgac ctttccagga
     8281 gctgggggac tgacgccaca ggacgacgat gcaaccgggt cgagggttgt ccagcgcttg
     8341 tccataagca agccatgagc gcgcggccgc attattcgta ccccgttggc gcgcttgcag
     8401 catgcaccag tttacgaaag cagagcacca gctcgtctca tcgttattgg cttgaaggct
     8461 tgtcgtattg aaatactcga cgacgcgcgg attatgacgc ggccccgggt aatccctctg
     8521 accaagttcg ccgcgcgcga ccgcgaacca cggcgcctcg ctctcggccg cagtctccat
     8581 cgcaactgag gcagccgcca ggcggaggcg agaccgatcc ggcgcttgca tgcttttccc
     8641 acccttggca agccgatcat agagcttgcg gatgtccgtg aaagtttcga tgtcgccaac
     8701 gccggcgaca ttcgcggctg tgacgagttc gcgttttcgc ggcgctcttt tccgcgtcga
     8761 ggtcttcgct gtggatttac gtgccatgac atcctccctg gataaaatac acgcacaatt
     8821 ataggttgtg atttggcata aatacagtag acggaagagg gctgattgcg accgcattat
     8881 gagtgcgcct gcaaaattcc tgcatgttcc agcaggaagc ggggctggtt gcttgaagtt
     8941 tctgcccgtg gaatgggctc gcacgcgccg ggccatcatc ttaacgccac aaccggtcgc
     9001 tagttgatcg tccctgtcgc aagggagagt ccattggcga aactcacgct ttatcacgcc
     9061 tcgccgtcac gctcctcgat cgtccgctgg atgctcgagg agttgggcga gccctacgac
     9121 cttcacgtct tgaggctcgc caatggcgag caacacgcgc cggattatct ggccgtcaat
     9181 ccgatgggca aggtgccggc tctccgtcat ggcgagacga tcgtcaccga ggccgcggcc
     9241 atctgcacgt atctcgctga tgagtttccg caggcagggt tgaacgtgcc gatcggtgac
     9301 gcgcgccgcg gggcctacct taaatggctg ttcttcgcgt cttgcatcga ggccgccgtt
     9361 accgatcgtg ctttcccgcg gcgcgatgaa ccgccccggg gcatgttggg ctatggagac
     9421 ttcgacacgg tgatgggcgt ggcggccaag gcggtcgagc ctggacccta tctgctgggc
     9481 gaacagttca ctgcggccga cgtggtgtta ggctcgacgc tgcgctgggg catgatgttc
     9541 aatcttctgc ccaagcgtcc gcagttcgtc gcctatgtag gccggctcga acagcggccg
     9601 gccttgcagc gggccaccgc tctcgaccag gagctggcaa acgcctagcg gccgaaagcc
     9661 tgtctgagcg ccgtcttggt ttgttcgagc ttggcgcgct gttccaccga aagcttggcg
     9721 ccggcgcgat tgacgtagaa gtccagcatc gacatggcgg tgcgataggg gtcgccgctg
     9781 tggcgggccg ggtcttccgc cgatttcttg agcgcggccg cgatctgctg cgccgagcca
     9841 ttggcgaaca gaccgggctc gagatcgaag ggatggtcat gcgcggtgag gtcggcggac
     9901 catttcttgc cggacttggc catgagtgcc tccttggggc ttccgaacgc gaggctcgca
     9961 cgcgatcatg atgatcgcac aaagaccatc gcgccaattt gcagcagttt gaagcgttga
    10021 tcggggcttg agcatgaaga tcctgtccgg cggccagaca ggcgtcgatc gggcggcgct
    10081 cgataccgcc atcgagaaag gccttcgcta tggcggctgg tgcccgaaag gcggctgggc
    10141 ggaggacctg cccgagccgc cggggctctt ggcgctatac cccgctttgc gcgagacgcc
    10201 gctcgccgaa cctgctcagc ggaccgaacg gaacgtgcgc gacgccgacc ggctcatgat
    10261 cctggtcgat agcgccggaa taagtgtgtc gaaagggacc gaagctgcgc gcgaccttgc
    10321 ctctcgtctc ggcaagccgc atgtcgtgct cgatctggat gcaggcagcg ttcaggtgcg
    10381 ggcacatacc tttctcggcg agggaaagga cggctttagc ctgtgcatcg ccggcccgcg
    10441 cgaaagcgag gcgccaggca tctacgccaa ggcccgcgcg tttctgcgcg aagttctcta
    10501 agcttccctc tgcccccgcg tgggagaggg aaaactcgcg ttggtcaggt atcatttcaa
    10561 acctccccct cgtggggagg tcggcacgct cgctctattt tgcgctaccg ctccgctacc
    10621 tgagcgcatt gcgcgccggg tggggggctg cgttcaaatc gcagccacgt cattccggcg
    10681 aaagccggaa tccagaagcc gcatgcactg cgcttgtttc tctggatacc ggcttctgcc
    10741 ggtatgacgg agtgttcacc ccacccggct cggccttcgg cctcgcctcc ctccccatcg
    10801 aggggagaga tgatttctcg ccgctggccg gccatttttg tatgtcgtct tagtcccgaa
    10861 tgggaaagcg agcttgaacc aggatagacc caagttaagc gtaacgcggc ggcaagttct
    10921 ggccggtgcg gccaccgccg cgtccacggt catcggcttg cgggctggaa cggcgcaaac
    10981 aaatccaacc ggatcgacaa ggccgaacgg caaagtctgc atcctcacgc cggaggcggt
    11041 cgagggtccg ttctatttcg atcccaagct cgtgcgcgcg gccgtcgccg aaggaaaggt
    11101 gggcgcaccc cttacggtcg ctctccaggt ggtggacgct gaaacctgcg ccgcgttcaa
    11161 cgaagcccgt gtcgatatct ggcattgcga tggactcggc gtctattcag gctatgcgcg
    11221 ccaggagacc ggctccgcca agggcgaaac atttctgcgc ggaacgcagt tcgctgcttc
    11281 cgatggcgcc gtgcgcttcg acaccatcta cccgggctgg tatccgggcc gcacgccgca
    11341 tattcacttc aaggtcatcc tggacgacaa ggaccttgtc accgaccagc tctatttccc
    11401 gattccgtca gaaagcacgt ctacgcaacg tattctccgt accgcgagcg caagcagcag
    11461 cgtgacaccg tgaacgccaa cgacttcatc tttctcgagc aacgcggcgc cgacacgctc
    11521 gccgataccg aagaagcggg cggctcctat cgcgcctccc tcgtcatcgg catcaaccgc
    11581 caaggccgcg cctagaagcg gggcggtcga tcttcttcaa ggattgcgtc agagaaagcg
    11641 atcgtcgtgc catcgccagg tcgcgtctcg caaagttcaa gaaggcaagg cagcgactcg
    11701 tgatagaatg acacattgat tcagcggagg ggaagacgat gctggaagca ctcatcattg
    11761 cctgtgtggt cggaggaatc gcgggttggc ttgcgggcct gctcgtgcag gggacgggct
    11821 tcggcctggt cggcgatatc gtcatcggca ttttgggggc gctgatcgcg gccttcctct
    11881 ttcccgccct cggcatcgcg ctcaccgtcg gcggcgggat tatcggcgcg atcattctcg
    11941 ccttcatcgg cgccgtgatc ctgcttgtca tcgtgaaggt gataaagggg atgacggcgt
    12001 aaccgcgctc gcgcagcttc gccgattctc ccacagcgaa ggctcactac catacctccc
    12061 cctcgtgggg aggtccggcg cggccggagc ttcgctcaag cttcgccctt ccaaaacctc
    12121 cccctcgtgg ggagatccgg cgcgcttttg cgcgctgggg tggagggttc ctgcgctaaa
    12181 acggggtagc cacgtcattc cggcgaaagc cggaatccag aagcggcatg cactgcgttc
    12241 gtgactctgg ataccggctt acgccggtat gacgaacgtc accccacccg gctcagcttt
    12301 cgctccgcca cgcgtggctc cgctcaagct caccgcacct ccccatcaag gagggaggat
    12361 tccgagtcgc cggagtgacg gtgtgcctca gaccgcgtct ttggcggcct tcaccgggcg
    12421 caccctgagc atcttgcggg cgggtttcgc cttgaagtcc gtcggctcct tggtgaacgg
    12481 gttgatgccc tggcgctctt tggtcgccgg cttcttgacg acgatgaact tggcaaagcc
    12541 cggaagcatg aaggtgccgc tctttctcag ctccttgtag gcaacgccgg cgagcccttc
    12601 catcaccgcc ttcacgtcct tcttcgccat cgagcactcg tcggcgactt tttgaatgag
    12661 ctcagatttc gacatcatgc gcgtgttcct cctttgccgg ccataggccc aatcttcgct
    12721 atggccttct tggcctcagt gcaatcccga ctttgcagca acgaacgtgc cggcgaaacg
    12781 gccccattcc tcgaaaactc gccgattcgg aggggtttgt cagcaaatac cccccaaaaa
    12841 ccccgcaaaa acgcatagtt tgtcgttgaa aagcgtgatt ttccgatgaa aagacgctga
    12901 ttgggcgaag ctccgctcgc acgctgtagg ggctcagcgc cagcagcatg cctctttgcc
    12961 gcgggggcag attagttcgc gagcgtcagc cgcaccctgt aggcgattgc gtggtcggcc
    13021 tcgatataga cgagctcgag cgtgtcggga gccgtgagcc gccctttgta gagaccgcta
    13081 tcgacgacag cggaaaatca ctcggggatg gcggcgcgcc tatcgccagc ctagctgctg
    13141 tgcccttgtt gcctcaccca gcgacccgtg tcagctcgaa cacgcgtccg tatttcgctg
    13201 cggcgtctac gtcgccacgc cgtaaagcaa gtctcacggc gtcgagtttc cgcgcatcct
    13261 cgaccgagag ataaggcgac cagccggttt cgtcttcgat gagctcaacc ggaacctcgg
    13321 cgacgtagtt cgcatcgcgg acgagttcga cggattttcg cttcctgatc gtgttctctc
    13381 taaagtcctc cgccctcact ccgcagcggc gcgcctgccg cccgcggcct tgcggcgccg
    13441 gtccatcacc tgcttgaggt attgcccgga gctctgaggc gcggcggcga tctcctcggg
    13501 cgtcccgacc gccacaaccg cgccgccggc gtcgccgcct tcaggcagct cgcgtcttta
    13561 tggacgcggg gctccgacgc aagcagtagc cgttcaattg aaaattgctg agccaatatc
    13621 ttcaattttg gcaaagccat tcctatttct ccagcccggt ccaactatcg aaaggagcca
    13681 gcatgacccc agacctaatg gcggggctct cagacccaac cgattatgcg cgcgaaccct
    13741 tggcccttga aactaccgga ataacagagc ttagcgctta atggttcaag gtgcctctca
    13801 acaaggttcg ttcaccgttg aaagagtgcc acgtcagttt aagacagcac gactggattt
    13861 gggtccattc gaattaagcg atgccaaaag agtggttgag ctggccggcg actgggacgt
    13921 tgcgcggtgg acggcttcca ttcctcatcc gtattcgatc gattcggctc aacggtggat
    13981 cgctcaccag gccgatcaaa ccaaagaact ggttttcgcg atccgatttg acgctgacct
    14041 tatcggatgc atcagctact cgattctcag tggagaaatc ggcttttgga taggaagagc
    14101 gtactcgaat caaggtttcg ccacagaggc gctagttggt ctggttcaaa cattgagagc
    14161 atgcgacttc acgaactttt gggcggcaac tttgccaagc aatagagcct cagcacgtgt
    14221 gttatccaag gctggatttt tgccagcggg attcttcgtg gtcgcgggcc gtctgagata
    14281 ctcgggaaaa cgcctcctta agtacgagtt ctaggctcat tcagcggctg cggcgcgctt
    14341 gccgcccgcg gccttgcggc gccggtccat gacctgcttg aggtattgcc cggtatagct
    14401 cgcagggttg gcggcgatct cctcgggcgt cccgaccgcc acaacctcgc cgccggcgtc
    14461 gccgccttca ggcccgagat cgacgatcca gtctgcggtc ttgatcactt cgagattgtg
    14521 ctcgatcacc accactgtgt tgccctgatc gaccagctcg tgcagcacct cgagcagctt
    14581 cgccacgtca tggaagtgaa gccccgtggt cggctcgtcg aggatataga gcgtgcggcc
    14641 cgtggcgcgg cgcgacagct cgcggctgag cttcacccgc tgcgcctcgc cgcccgaaag
    14701 cgtcgtcgcc tgctgcccga catggatata ggagaggccg acgcgcttca gcgtctcgag
    14761 cttgtcgcgc accgcgggca ccgccttgaa cagctccgcc gcctcctcga ccgtcatgtc
    14821 gagcacgtcg gcgatcgatt tgcccttgaa cttgatctcc agcgtctcgc ggtcgtagcg
    14881 cttgcccttg cacacgtcgc aggtgacgta gatgtcgggc aggaagtgca tctcgatctt
    14941 gatgacgccg tcgccctgac aggcctcgca gcgcccgcct ttgacgttga aggagaagcg
    15001 ccccggcttg tagccgcgtg tcttggcctc gggcagctcg gcgaaccact cgcggatcgg
    15061 ggtgaaggcg ccggtatagg tggccgggtt cgagcgtggt gtgcgcccga tcggcgactg
    15121 gtcgatgtcg atcaccttgt cgagatgctc cagtccctcg atcgcgtcat gctcgcccgg
    15181 cacctcgcgc gcgcctgaga gcctgcgggc gacggcgcga tagagcgtct cgatcaggaa
    15241 ggtggacttg ccgccgcccg agacgcccgt gatgcaggtg aacaggccga gcggaatatc
    15301 gacggtgatg tccttgaggt tgttggcccg cgcgccgagc acggagagcc gccggcccct
    15361 ggtcggcttg cgcctggcct tgggcgaggg gatctgcttg aagccggtca gatattggcc
    15421 ggtgaggctg gccggcgccg ccatcacctc gtcgggcgtg ccttccgcca ccacctcgcc
    15481 gccatggatg cccgcgccgg gccccatgtc caccacatgg tcggcggtca ggatcgcctc
    15541 ttcgtcgtgc tcgaccacga gcacgctgtt gcccatgtca cgcagcgact tcagcgtatc
    15601 gagcaaccgc gcattgtcgc gttggtgcag cccgatcgac ggctcgtcga gcacgtagag
    15661 cacgccggtc aagcccgatc cgatctgcga ggcaagccgg atgcgctgac tctcgccgcc
    15721 cgacagcgag cctgaggcgc gcgacagcgt gagataatcg aggccgacat cgttgaggaa
    15781 agtgagccgc tcgcgaatct ccttcaggat gcgcgcggcg atctcgttct gcttcggcgt
    15841 gaggtgaacg ggaaggtcgc cgaaccagca cgccgcctcc ttgatcgaca tctccgtgac
    15901 ctggccgata tgcagccgcg cgatcttcac ggcgagcgcc tgcggcttca ggcgatagcc
    15961 gccgcagact tggcagggat ggttcgactg gaaacgcgac aactcgtcgc gcacccacga
    16021 ggactcggtc tcgcgccagc gccgctcgat attggtgatg acgccttcga acggcttggt
    16081 ggtcgagtaa tggcgcacgc cgtcgtcata gctgaaggtg atctcgtcgt cgcccgagcc
    16141 gtagagcacg atgtcgcgga acgccttggg caggctcttc cacggcttgg tcatgggctg
    16201 gcgataggcg cgggcgagcg attccagcgt ctgctggtaa tagggcgagg tggccgaggt
    16261 gcgcgcccag ggggcgatgg ccccacggtt gaggctgagg tcctcgtccg gcacgaccag
    16321 gtccggctcg aagaacagct cggtgccgag cccgtcgcag gcggggcagg cgccatag
//
LOCUS       DASUQP010000215         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_923871,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000215 DASUQP010000000
VERSION     DASUQP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923871"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VFY74_09720"
     CDS             <1..118
                     /locus_tag="VFY74_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX5932010.1"
                     /translation="DEDWQISQWGEDAEAAARRAARWRDFAAAAQVLTLLKP"
     gene            474..2006
                     /locus_tag="VFY74_09725"
     CDS             474..2006
                     /locus_tag="VFY74_09725"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX5932011.1"
                     /translation="MKEVLEELEFRRDKARVGGGAARIEAQHARGKLTARERLDLLLD
                     EGSFEEFDMYVEHRCIDFGMEQNRVPGDGVVTGWGTINGRVTYVFAKDFTVFGGSLSE
                     AHAEKMVKIQDMALRNRAPIIGLFDAGGARIQEGVAALGGYGGVFLRNVLASGVIPQI
                     SLIMGPCAGGDVYSPAMTDFIFMVKDTSYMFVTGPDVVKTVTKEEVTAEELGGAVVHT
                     SKSSIADGAYENDVEALLQMRRLMDFLPSSNLSGVPELPTRDPWDRMEKSLDTLIPDN
                     PNKPYDMKELIHKVVDEGDFFEIQESFARNIVVGFARMEGRTIGVVANQPMVLAGVLD
                     SDASRKAARFVRFCDCFNIPLITFVDVPGFLPGTDQEYGGLIKHGAKLLFAFTEATVP
                     KVTVITRKAYGGAYDVMSSKHIRGDVNYAWPSAEIAVMGAKGAVEILYRAELSDPAKI
                     KKRIDEYQARFANPFVAAERGYIDEVILPHGTRKRIARALQMLRNKRVENPWKKHDNI
                     PL"
     gene            complement(2441..2836)
                     /locus_tag="VFY74_09730"
     CDS             complement(2441..2836)
                     /locus_tag="VFY74_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932012.1"
                     /translation="MIETLRQIIKPKPQAQLSEGGSDATIESVALAGAAAIQRLIADR
                     DNLRDRVGAQQRELVTLTAANEELRCRLGLIRQQYLELGSRILAQLEQFDRVTREAIQ
                     DNQVPASTPQDDANLIALAHRLKPNSGPA"
BASE COUNT          508 a          935 c         1006 g          536 t
ORIGIN      
        1 cgatgaggac tggcagatca gccaatgggg cgaggatgcg gaggctgcgg cgcgacgcgc
       61 ggcgcgctgg cgcgatttcg ccgctgccgc ccaagtgctg accctgctca aaccctgact
      121 ctaacctccc cctcgtgggg aggtccgacg cggccgaagg ccgcggtggg gtggggggtg
      181 cgcttggatg tgagggggag aatggagtcg gcgcggcggg tcatcggtca ttgccgggct
      241 tgatccccgg atcaagtccg gggacaggct ccggcaatcc attccattgg cgcttcaact
      301 ggctgaaccg caacggcatg gatgcccgga caagcttgcg cccgggccgg cgaagccgga
      361 cccgggtgtc cgggcatgac gaagggtgcg ggcgggaggt actggccggc atttgcgcca
      421 ttttaacccc gtgctatgca ccgtcacgga gtcgtcaccc tgaggaagcg gccatgaagg
      481 aagttcttga agagcttgag tttcgccgcg acaaggcgcg ggtcggcggc ggcgcggccc
      541 gcatcgaggc gcaacacgcc cgcggcaagc tcaccgcgcg cgagcggctc gatcttctgc
      601 tcgacgaagg ctccttcgag gagttcgaca tgtatgtcga gcaccgttgc atcgatttcg
      661 gcatggagca gaaccgcgtg ccgggcgacg gcgtggtcac cggttggggc accatcaacg
      721 gccgcgtcac gtatgtgttc gccaaggact tcaccgtgtt cggcggttcg ttgtccgagg
      781 cgcatgccga gaagatggtc aagatccagg acatggcgct caggaaccgc gccccgatca
      841 tcggcctgtt cgacgccggc ggggcgcgca tccaggaggg cgtggcggcg ctcggcggct
      901 atggcggcgt gttcctgcgc aacgttctcg cctcgggcgt gatcccgcaa atctcgctga
      961 tcatgggacc ttgcgccggc ggcgacgtct attcccccgc catgaccgac ttcatcttta
     1021 tggtcaaaga cacctcctac atgttcgtca ctggcccgga cgtggtgaag accgtgacga
     1081 aggaggaggt caccgccgaa gagctcggcg gggcggtggt gcatacctcg aaatcgtcga
     1141 tcgccgacgg cgcctacgag aacgacgtcg aggcgctgct ccagatgcgg cggctgatgg
     1201 attttctgcc gtcgagcaat ctctcaggcg tgcccgagct tcccacccga gacccgtggg
     1261 accggatgga gaagtcgctc gacacgctga ttcccgacaa cccgaacaag ccctacgaca
     1321 tgaaggagct gatccataag gtcgtcgacg agggcgactt ctttgagatc caggagagct
     1381 tcgcccgcaa catcgtggtg ggcttcgccc gcatggaggg gcgcacgatc ggcgtcgtcg
     1441 ccaatcagcc gatggtgctc gccggcgtgc tggactccga cgcctcacgc aaggcggcgc
     1501 ggttcgtgcg cttctgcgac tgtttcaaca tcccgctcat caccttcgtc gacgtgcccg
     1561 gcttccttcc cggcaccgac caggagtacg gcggcctcat caagcacgga gccaagctgc
     1621 tcttcgcctt caccgaggcg acggtgccga aggtgacggt catcacccgg aaggcctatg
     1681 gcggcgccta cgacgtcatg tcctcgaaac atattcgggg cgacgtgaac tacgcctggc
     1741 cgtcggccga gatcgcggtg atgggggcca agggcgcggt cgagatcctc tatcgcgccg
     1801 agcttagcga tcccgccaag atcaagaagc gcatcgacga gtatcaggcg cgcttcgcca
     1861 atccgttcgt ggcggcggag cgcggctata tcgacgaggt gatcctgccg cacggcacgc
     1921 gcaagcgcat cgcaagagcg ctccagatgc tgcgcaacaa gcgcgtcgag aacccctgga
     1981 agaagcacga caacatcccg ctttagtccc acccttcgag acgggccttc ggccctcctc
     2041 ggggtgaggt gatcacaaca ccaacgccct catgctgagg agcgccggta agggcgcgtc
     2101 tcgaagcacg cactcaattt ccctctcccc ttgagggaga gggcttcccg cccggcgcgc
     2161 gaagcgcgac gaatggcggg aagggtgagg gggtgatttt ttcttcgacg ccgcttcgtg
     2221 gctgaccccc tcaccgcgag cgcgaagatg cgctcgcgtg ctctccctcg aggggagagc
     2281 gtgcccggca tcgcccgaag ggcgaagccg ggcgggcgag ggggctttct tgttgacgcg
     2341 ggcttcgccc gctgtcccct caccacgcgc caagacgcgc gtgtgctttc cctcaagggg
     2401 agagcgggaa caggcggcga gcacccggtg cttacttagg ttacgccggt ccgctgttcg
     2461 gcttgaggcg gtgggcaagc gcgatgaggt tcgcgtcgtc ctggggcgtg gaggcgggca
     2521 cctggttgtc ctggatcgct tcgcgggtga ccctgtcgaa ctgctcgagc tgcgccagga
     2581 tccggctgcc gagctccaaa tattgctggc ggatcaggcc gaggcgacag cgaagctctt
     2641 cgttcgcggc agtcaaggtc actaactcgc gctgttgagc gccgacgcga tcccgcagat
     2701 tgtcgcgatc ggcgatgagg cgctggatcg ccgccgctcc ggcgagagcg acgctctcga
     2761 tcgtcgcatc ggacccgccc tccgacaact gggcttgcgg cttcggcttt atgatttgtc
     2821 gcagggtctc aatcatgtgc aacgaactcc cttttatcgg tggacggggg cggccatcag
     2881 cttcggatca tgcttggggg cactctcgcg ccaaattgtg cagttgcgcg gcctctgccg
     2941 agcttttctt tgtccgatgc ttaatcgggg cattaactga gttga
//
LOCUS       DASUQP010000216        14696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1195984, whole genome shotgun sequence.
ACCESSION   DASUQP010000216 DASUQP010000000
VERSION     DASUQP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14696
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195984"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFY74_09735"
     CDS             <1..252
                     /locus_tag="VFY74_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932013.1"
                     /translation="IKAAEAAGVPQYTGLHCLRHFYASWCLNRKEDGGLGYSFQQVKE
                     LMGHSSITITVDRYGHLFENGDPNTLEAAEQALMALHAQ"
     gene            522..884
                     /locus_tag="VFY74_09740"
     CDS             522..884
                     /locus_tag="VFY74_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEX5932014.1"
                     /translation="MVERVLATDATLELLERLKRKHGALMFHQSGGCCDGSAPMCYPA
                     TEFRVGGNDVRLGEIGGCPFFMDADQFDYWKHTQLIIDVVPGRGHGFSVEAPEGVRFL
                     TRSRVFTDEEVDALAASA"
     gene            complement(1313..1768)
                     /locus_tag="VFY74_09745"
     CDS             complement(1313..1768)
                     /locus_tag="VFY74_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX5932015.1"
                     /translation="MGIQAEILGRAGQAGRTGDAAAFAFLRDLGAGMSKEERLAQVRW
                     LARMLDDSFGIPGTGIRFGWDSVLGFVPGLGDALTTALALVIVHHAWQSGASKITLAR
                     MLGNVAADFAIGAVPVLGDLFDFAFKANRRNVRLLEQHLNKPASKHRPR"
     gene            complement(1867..3615)
                     /gene="ybaL"
                     /locus_tag="VFY74_09750"
     CDS             complement(1867..3615)
                     /gene="ybaL"
                     /locus_tag="VFY74_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEX5932016.1"
                     /translation="MPHYTPLITTIVAALTLAWGFAAVAYRLKLPPLVGYLLAGIAIG
                     PFTPGYVADQDLAGQLAEIGVILLMFGVGLHFSLEDLLSVRKIAIPGAIVQILCATLL
                     GMGLAAWLGWSFGAGLVFGLALSVASTVVLLRAMQERRLIETERGRIAVGWLIVEDIA
                     MVLTLVLLPVVAGLLTGGDGGGADLADIALALALTGAKIAAFVAVMLIVGKRVIPWIL
                     HVMADTGSREMFRLSVLVIALGTAFGAAELFGVSFALGAFFAGMILSESKLSQQAATE
                     TLPLRDAFAVLFFVSVGMLFDPAILIREPVPVVATLLIIIVGKSLAAYGIVRAFGYSN
                     ATALTISASLAQIGEFSFILANLGVDLQLLPEPGRDLILAGAIISIILNPFLFSALDW
                     ISARQEIQHAKAPSARASEAGPRERGGGDETGPREMVEPTTLKNHVVLVGHGRVGRAI
                     SAALRAQKAPLFVIEDDEEAVAALKQQGIEALAGNAADPELIAAANLPAARCLLVAIA
                     DGFEGGQVVEQARAINPGLAIIARAHSDEEMEHLKRHGATVVVMGEHEIAKSMLDNIA
                     EAAPKRAKPRRRARSS"
     gene            complement(3629..5374)
                     /gene="ybaL"
                     /locus_tag="VFY74_09755"
     CDS             complement(3629..5374)
                     /gene="ybaL"
                     /locus_tag="VFY74_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEX5932017.1"
                     /translation="MPHVPPLVATIALGLVLAFSFGVVAHRLKLPPLIGYLVAGILIG
                     PFTPGPVADQNIANQLAEVGVILLMFGVGLHFSLKDLLSVRAIAIPGAVAQALIATPL
                     GIAVGLWLGWSLAAGLIFGIALSVASTVVLLRVLQERRLLETERGRITVGWLIVQDIA
                     MILVLVLLPGLAALFHGMQTAPKAAEIALSVTMLLAKFGVFIAVMMIIGKKLIPAILH
                     YVAHTGSRELFRLAVLSIALGVAYAAAELFGISLALGAFFAGMMLSESALSQQAATES
                     LPLRDAFAVLFFVSVGMLFNPAIVVDAPLALIATVLIIVFAKSVAAYFVVRLFAHSHP
                     TALMISASLAQIGEFSFILANRSLDLGVLPVEGRDLILAGAIITILLNPFLFAALEIY
                     APRREKPPEPAADQEEDETPMREPIRPTSLTDHVVLVGHGRVGGFISAALRERNVPLF
                     VIEDDDERVAALRQLGVEAIAGNAADPEVIQAANLGSARCLLVAIPDAFEGGQVVRQA
                     RIINATLPILARAHSEEEIAHLKQHGATVVVMGEHEIAKSMLDDAAVASAAKQAATAA
                     EPSRPVLVEKPVATS"
     gene            5545..5793
                     /locus_tag="VFY74_09760"
     CDS             5545..5793
                     /locus_tag="VFY74_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932018.1"
                     /translation="MSAFDYRAPAELYASASKTGKRPMRYTRFKSAAEAIKYAVEELP
                     PELLIGSTLEVNEERFDGYGIRALYDSANYPLRRRKTS"
     gene            5915..6160
                     /locus_tag="VFY74_09765"
     CDS             5915..6160
                     /locus_tag="VFY74_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932019.1"
                     /translation="MDHKRSCLGLLAGCLWLGAIAPAPAADLGHGESMPAGWEFSLTP
                     YGWMINVNGDVTARGNTVEVNQNFSRSSKKAIRSWPG"
     gene            6157..6822
                     /locus_tag="VFY74_09770"
     CDS             6157..6822
                     /locus_tag="VFY74_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932020.1"
                     /translation="MSYFEARKGKLALFTDLVWLDLGFPGKIDVRGSPFARFPRVVVS
                     VDGKAQLDYESIIVQPGVAYEVARWQSAPGSYTALDLLGGARYWNQDVSLSLRLTGTL
                     EVDLERLGLKFKRSRRVAIARADDLEWVDPVVGARVRHQIAPGKELRLEGDIGGFGVG
                     SEFAWQAVGVYSFHTKCLGVPLHADIGYRALYVDYSEDGRFGKQGLDLVQHGPVMGVT
                     FNW"
     gene            6976..7470
                     /locus_tag="VFY74_09775"
     CDS             6976..7470
                     /locus_tag="VFY74_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932021.1"
                     /translation="MVATVEAIDVTNQLVTVVGPNNNWVVVKVGPEHIKLVKVKERIT
                     ISYADEVAVALRKYAGPPKATENPIAKDELADMNMNPPTMAEQDWVEATPHGAKELTT
                     IEISDTVAAVNRNRRTITFFGTGGKTRTIAIAPNVPGFEQVEVGDRIVLLVTRAVAVD
                     IRPV"
     gene            complement(7555..8172)
                     /locus_tag="VFY74_09780"
     CDS             complement(7555..8172)
                     /locus_tag="VFY74_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX5932022.1"
                     /translation="MAKRPFHAVSALAGLAVPTRLGEGRGDPGVTIEERVGLGLATVA
                     ARKGQAEALKGAVASAYGVELPDGSRVAHGPSISFVGYGPGQWLAVSESLVNETLARD
                     LGQRLRGLASISDQSGGRAVLRVSGPRARAVLAKGLPIDLHPRAFTPGSAATSTISLM
                     GVQLWQVDDAPTYDLAIFRSVSASFWRWLTASAGEFGYEVVGRER"
     gene            complement(8165..11143)
                     /locus_tag="VFY74_09785"
     CDS             complement(8165..11143)
                     /locus_tag="VFY74_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HEX5932023.1"
                     /translation="MTTARQPNRLGEGGLIDRSQTVRFEFDGAPLAGHPGDTLASALL
                     ANGIRLVGRSFKYHRPRGILTAGSEEPSALVELRTGARREPNTRATMIELYDGLSAAS
                     QNRWPSVKFDVLAANQLFGPIFSAGFYYKTFMWPPTFWEKVYEPMIRRAAGLGRGAGV
                     ADPDHYEKAYAFCDVLVVGSGPAGLAATLAAARSGARVILCEEDFRLGGRLLAERYEI
                     DGKPARDWVETVEAELEAQPECRILRRTTVQGVYDGGTYAALERVNDHVLKPLAHEPR
                     QRLWRIVAKRVVVCAGALERPIVFGNNDRPGVMLAGAVRSYVNRFGLAPGRRAVIFAD
                     NDDAFRTASDLAQAGIEIAAAVDGRRTPSRAAQEAAEAAGARYRAVAVIERVRGLHGV
                     KGVEIRDEKGRERMACDLIAVSGGWNPTLHLTTHLNAKPQWNDRLAALVPGALPPGMS
                     VAGAARGTYGLAACLAEGEEAGAKAASECGFAASASRPRASDESVTASPLWQVAESKG
                     KAFIDFQNDVAVSDVKLAACEGFRIPEHLKRYTTLGMATDQGKTGNANGAAVLAAVTG
                     RYIGAVGTTTFRPPYTPVAISAFAGHHRGPDFRPARLAASHGFAEEQGAVFVETGPWL
                     RALYYPKPGEEPQDSVNREVATVRGQAGVCDVSTLGKIDVQGKDASIFLDRVYANTFS
                     TLPVGKARYGLMLREDGFVLDDGTTARLAEDHYFMTTTTVNAVKVMQHLEFCQQALWP
                     ELDVQTVSVTEQWAQFAVAGPRARDVLQGVVDPAYDLSNAAFPYLACGEVTVAGGVPA
                     RLYRLSFSGELAYEIGVPARFGEALIRAIMEAGAPFGLAPYGTEALGVLRIEKGHPAG
                     GELNGQTTAHDLGFGKMLSTKKDFIGRFMAAREALTDPLRPTLVGLRPLAREDRVRAG
                     AHLIPKDKEATARNDQGYVTSIAFSPSLGHWLGLGLLSRGPERHGEIVRAYDHVRGGD
                     ILVEVRPPCFIDPEGERVRG"
     gene            complement(11140..11409)
                     /locus_tag="VFY74_09790"
     CDS             complement(11140..11409)
                     /locus_tag="VFY74_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX5932024.1"
                     /translation="MRIRCPYCGERALEEFTYRGDATVRRPDSLDPGAAQAWVDYVYL
                     RDNPAGLHKEYWHHSAGCHAWLVVTRNMSTHEIVAVEFAEKGASK"
     gene            complement(11420..12670)
                     /locus_tag="VFY74_09795"
     CDS             complement(11420..12670)
                     /locus_tag="VFY74_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX5932025.1"
                     /translation="MRYSVASLLANACTGNRYWKPLWREPELKPSYDVVIVGGGGHGL
                     ATAHYLAKKHGITNVAVIEKSYLGSGNVGRNTTIVRSNYLLPGNLPFYEWSMKLWEGL
                     EHDLNYNTMVSQRGVLNVFHSDPQRDAYARRGNAMRLHGVDAELLDREGVRALVPFVD
                     FDNARFPVRGGLLQPRGGTVRHDAVAWGYARAADSRGVDLIQNCAATGFRIEGGRVRG
                     VETEKGFIAAKKVALACAGNSSRVAAMAGLRLPIESHSVQAFVTEGVKPVIDVVMTFG
                     AGHFYVSQSDKGGLVFGGDIEGYNSYAQRGNLPVIEDVCEGGMALMPVIGRLRILRSW
                     GGLLDMSMDGSPIIDTTPIDGLYLNAGWCYGGFKATPASGWCFAHLIATGEPHPLAAA
                     YRLDRFAQGRVIDEKGMGAQPNLH"
     gene            12740..13744
                     /locus_tag="VFY74_09800"
     CDS             12740..13744
                     /locus_tag="VFY74_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007698431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX5932026.1"
                     /translation="MSRKQAAPQAAEEGTIPVKQPAARTVGFLLVPGFALMSYAAAIE
                     PLRAVNLLSGRELYRWWHAAPGGKPVLASNGVAIIPDCGTNTERAADMVFVCAGGNPA
                     LFEDRSVFAWLRRMARKGVAIGGISGGPYVLAKAGLLDERRATLHWEHLPAFREAFPD
                     VAVVPSLFEIDGNRITCSGGISALDMMVAIIEKEHGRQLAAAVGDWFLHTHVREGFGP
                     QRMDLRYRLGIADEKLLAVLRAMEASIETPLSRFSLAREAGISLRQLERLFRRHIGHG
                     IHRHYRWLRLERARQLLRETTLPVLDVALATGFASASQFARAYAGAFGEPPSRTRISG
                     "
     gene            complement(13761..14171)
                     /locus_tag="VFY74_09805"
     CDS             complement(13761..14171)
                     /locus_tag="VFY74_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932027.1"
                     /translation="MRPLALLLLLLPASALAEERPVRIDTNPPYVQVVVTACPAAETP
                     LEPTNHGKSYENERGMTREERNAYFREIGCIEVPIPPEWIAQELSYRQCIGPAGYLVS
                     MQYLEQNKTLVRTPAVGQWACIPGGTPFVGVASQ"
     gene            complement(14177..14368)
                     /locus_tag="VFY74_09810"
     CDS             complement(14177..14368)
                     /locus_tag="VFY74_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932028.1"
                     /translation="MSAVTASVTESPKTWNPVVVAGALRKVVNGLFDPYRPERHYMRG
                     PGPRWREQHAFDGAYPPRG"
BASE COUNT         2660 a         4750 c         4850 g         2436 t
ORIGIN      
        1 atcaaggcgg cggaagctgc gggcgtgccg caatacactg gcctgcactg cttgcgtcac
       61 ttctatgcaa gctggtgcct gaaccggaaa gaggacggcg gcctcggcta tagcttccag
      121 caagtgaagg aactgatggg ccatagctcg atcaccatca ccgtggatcg ctacggccac
      181 ctgtttgaga acggcgaccc caatacactg gaggctgcgg agcaggccct aatggctctg
      241 catgcgcagt gatttgtaat ataatgactc tgcatgcaac atagatgcaa catgaagcta
      301 gaatagtaag cgaaatcaac tcttgtgagc aatccaagac atgctggtga gctatagccc
      361 caaggcgatg ggcttcttct aggcgaagcg cagtaacagc acttgtcgtc atcgccgggc
      421 ttgacccggc gatcgttccg ctgtcgtttc accgcgacga ggcgtatcgg aatagatgcc
      481 cgggtcaagc ccgggcatga cgaggagagt gggaaccagc catggttgag cgggtcctgg
      541 ccactgatgc gacgctagag ctactcgaac gcctcaagcg caagcacggg gcgctgatgt
      601 tccatcagtc gggcggctgc tgcgacggct cggcgccgat gtgctatccg gcaaccgagt
      661 tccgcgtcgg cggcaacgac gtgagactcg gcgagatcgg cggttgtccc ttctttatgg
      721 acgccgacca gttcgattac tggaagcaca cccagctcat catcgacgtg gtgccggggc
      781 gcgggcacgg cttctccgtc gaagcgcccg aaggcgtgcg cttcctcacg cgaagccgtg
      841 tctttaccga cgaagaggtc gacgcgctcg ccgcctccgc ctgacgacac tgtcagcgcg
      901 gccggcgctt cgaagccggc ccgttcaaat gctgctccag cagtcgcaca ttgcggcgcg
      961 gccttcggcg gctataagac gtcgggcatc gcgcgcgaga cgcacaagat gatgctcgac
     1021 catcaataag tgaaagggcc ccctggccaa tgggggccct aaacttcatc gcggcctggg
     1081 tgacaactcc gttacgcgct acgcgatact ggccccggag accgctctat atgaacggat
     1141 gtaggaagcc gggcgccggt tttcaactcc gggcacaacc cgcacggatt tagcagagga
     1201 tatggggcgc tcgcggtggg cgccccgccc gagggcgtgc gcttcctcac gcggagccgc
     1261 gtcttaccga cgaagaggtc gacgcgctcg ccgcctccgc ctgaggcgaa tgtcagcggg
     1321 gccggtgctt tgaagccggc ttgttcagat gctgctccag caggcgaacg ttgcggcggt
     1381 tggccttgaa ggcgaaatcg aacagatcgc cgagaacggg cacggcgccg atcgcgaaat
     1441 cggcggcgac attgccgagc atgcgcgcga gcgtgatctt cgaggcgccg ctctgccagg
     1501 catgatgcac gatgacgaga gcgagcgcgg tggtgagtgc gtcgcccagt cccgggacga
     1561 agccgagcac ggaatcccag ccgaaacgga tgccggttcc gggaattccg aaactgtcgt
     1621 caagcatgcg ggcgagccag cgcacctgcg cgagccgctc ctccttgctc atgccggcgc
     1681 cgagatcgcg caggaacgcg aaagccgcgg cgtctcccgt ccgccccgcc tgcccggccc
     1741 ggcccaagat ttcggcttga atacccatca cgctgcttct gtctcatgaa tcgatcacgg
     1801 ggccggatat gggggctcga tgcccggcat caaccccaag cgcttcgccg aaatcaaatc
     1861 gaagctttac gaggagcggg cgcggcgacg cggtttcgcg cgtttgggcg cggcttcggc
     1921 aatattgtcg agcatcgact tggcgatctc atgctccccc atcaccacga cagtggcgcc
     1981 atgccgcttg agatgttcca tctcctcgtc cgaatgcgcg cgggcgataa ttgcgaggcc
     2041 gggattgatg gcgcgggcct gctccaccac ctgaccgcct tcaaagccgt cggcaatggc
     2101 gacgaggagg cagcgcgccg cgggcaaatt tgctgcggca atcaattcgg ggtcggcggc
     2161 gttgccggcc agcgcctcaa tgccctgctg cttgagtgcc gcgaccgcct cttcatcgtc
     2221 ctcgatgaca aagagcggcg ccttctgcgc gcgcaaggcg gcgctgatgg cgcgaccgac
     2281 ccggccgtga ccgaccaaca ccacgtggtt cttgagggtg gtgggctcga ccatctcgcg
     2341 cgggcccgtt tcgtcgcccc cgcctcgctc gcgcggaccg gcttcgcttg cgcgggctga
     2401 cggcgctttg gcgtgttgga tttcctgccg ggcggatatc cagtcgagcg cggaaaacag
     2461 gaatgggttc aggatgatgg agatgatggc gccggcaagg atgaggtcgc ggccgggctc
     2521 tggcagaagc tgcaaatcga cgccgagatt ggcgaggatg aaggagaatt caccgatctg
     2581 ggccagtgaa gccgaaatgg tgagcgcggt cgcgttggaa taaccgaaag cacgcacaat
     2641 gccgtaagcg gccagcgact tgcccacgat gatgatgagc agcgtcgcca cgaccggcac
     2701 cggctcccgt atcagaatgg cgggatcgaa cagcatgccc accgagacga agaacagcac
     2761 ggcgaaggcg tcgcgcagcg gcaaggtctc ggtcgcggcc tgctggctga gcttcgactc
     2821 gctcaagatc atgccggcga agaaggcgcc gagcgcgaac gacacgccga acagctcggc
     2881 cgccccgaac gccgtaccga gcgcaatcac gagcaccgac aggcggaaca tctcgcgcga
     2941 cccggtatcc gccatgacgt gaaggatcca gggaatgacc ctcttgccga cgatcagcat
     3001 gaccgcgacg aaagccgcga tcttggctcc ggtcagagcg agcgcgagcg cgatgtccgc
     3061 aagatcggcg ccgcccccgt ccccgcccgt aagcaggccg gcaacgacgg gcagaaggac
     3121 gagggtgaga accatcgcga tgtcctcgac gatgagccac cccacggcga tccgaccgcg
     3181 ctcggtctcg atgaggcgcc gctcttgcat cgcgcggagc agcacgacgg tgctcgccac
     3241 cgagagcgcg agcccgaaca cgagcccagc cccgaacgac cagccgagcc acgccgcaag
     3301 ccccatgccg agcagggtcg cacacaggat ctgaacgatg gctccgggaa tggcgatctt
     3361 cctgaccgag agaagatctt cgagcgagaa gtgcagtccc acgccgaaca tcagaagaat
     3421 gacgccgatc tccgccagct gtccggcgag atcctggtcg gcgacatagc ccggcgtgaa
     3481 cggcccgatg gcgatgccgg cgaggagata gccgacgagc ggcggaagct tcagccgata
     3541 cgccacggcc gcgaaccccc aggcgagtgt cagcgcggcg acgatggtcg tgatgagcgg
     3601 cgtataatgc ggcatggaag agccggacct agctcgttgc taccggcttc tccactaaca
     3661 cagggcggga cggctcggca gcggttgcgg cttgcttggc cgcgctcgcc accgccgcgt
     3721 cgtcgagcat cgacttggcg atctcatgct cccccatcac cacgaccgtg gcgccgtgct
     3781 gcttgaggtg ggcgatctct tcctcggaat gcgcccgggc gaggatcggg agcgtcgcat
     3841 tgatgatgcg ggcctgccgc accacctggc cgccctcgaa ggcgtccgga atggccacga
     3901 gaaggcaacg cgccgagccc aaattcgccg cttggatcac ctccggatcg gcggcgttgc
     3961 cggcgatcgc ctcgacgcca agctgccgta gagccgcgac cctctcgtcg tcatcctcga
     4021 tcacgaagag gggaacgttc cgctcccgta aggccgcgct gatgaaaccg ccgacccggc
     4081 catggccgac cagcaccaca tggtcggtaa gcgaggtcgg acggatcggc tcgcgcatcg
     4141 gcgtttcatc ttcctcttgg tcggccgctg gttcgggggg cttctcccgg cggggagcgt
     4201 aaatttcgag cgcggcaaag aggaacggat taagcaagat ggtaatgatg gccccggcga
     4261 gaatgagatc gcgtccctcg accggcagca cgccgagatc gaggctgcga ttggcaagaa
     4321 tgaaggagaa ctcgccgatc tgcgcgagcg aagcggaaat catcagtgcg gtcggatggg
     4381 aatgtgcgaa caggcgcacc acgaaatagg ccgcgaccga cttggcgaat acgatgatca
     4441 gaacggtcgc gatcagcgca agcggcgcgt cgaccacgat cgcaggattg aacagcatgc
     4501 cgacggagac gaagaacagc accgcgaagg catcgcgcaa aggaagcgac tcggtcgcgg
     4561 cctgctggct cagcgccgac tcgctcagca tcatgccggc gaagaaggcg ccaagggcga
     4621 gcgagatgcc gaacagttcc gcggcggcat aggcaacgcc aagcgcgatg gagagaaccg
     4681 cgaggcgaaa cagttcccgg ctcccggtgt gagctacgta gtgcagaatc gccggaataa
     4741 gcttcttgcc gatgatcatc atgacggcga tgaagacgcc gaacttggcg agcagcatgg
     4801 tgactgaaag cgcgatctcg gcggccttcg gcgccgtttg cattccgtgg aacagcgccg
     4861 cgagccccgg gagcaggaca agcaccagga tcatggcgat gtcctgcacg atcagccagc
     4921 cgacggtgat ccgccccctc tcggtttcca gcaaccgtcg ttcttgcagc acgcgcaaca
     4981 gcaccaccgt gctcgcaacc gaaagcgcga ttccgaaaat gagcccggcc gcgagcgacc
     5041 agccgagcca taagccgacc gcgatgccga gcggcgtggc gatcagggct tgcgccacgg
     5101 cgccggggat ggcgatggcg cgcaccgaaa gcaggtcttt gagcgagaag tgcagcccca
     5161 ccccgaacat caacagaatg acgccgacct cggcgagctg gttggcgata ttctggtcgg
     5221 cgaccggccc cggggtgaac ggcccgatga gaatgcccgc cacgagatag ccgatcagcg
     5281 gcggcagctt cagccgatgg gcgacgacgc caaagctgaa ggcgagcacg aggccgagcg
     5341 cgatggtggc aaccaggggc gggacgtgcg gcatgaaaca gaccttcgac cggcaggcgg
     5401 agctccgcgc cagcctgcta ccctagcctg acaatgaaag gcgcggccga caatcccttc
     5461 gcgcgctaca caacaacgaa atgcgatggg cgtggcgtcc tcccaggctg gccttgccgt
     5521 ctccgccatg ctttaatggc gcccgtgagt gcgttcgatt acagggctcc cgccgagctt
     5581 tacgccagcg cgagcaagac cgggaagcgg ccgatgcgct atacgcgctt caagagcgcc
     5641 gccgaggcca tcaaatacgc cgtcgaggaa ttgccgcccg agcttctcat cggaagcacg
     5701 ctcgaggtca acgaggagcg gttcgacggc tacggcattc gtgcccttta cgacagcgcg
     5761 aactaccccc tccggcgccg aaaaacgagc taaatccggg ctttgcagct gatttccgcg
     5821 aaaaattttc cgatctgttg ccggtttgca caagctgttc cgtgccagga atgctatagg
     5881 atcgccgagc cgcggcatcg gggggaatct gatcgtggac cataagcgtt cctgtctcgg
     5941 gctgctcgca ggctgcctct ggctcggcgc cattgcgcct gcgcccgcgg ccgatctcgg
     6001 tcatggggag tccatgcccg ccggctggga gttcagcctc acgccctatg gctggatgat
     6061 caatgtcaat ggcgacgtca ccgcccgcgg caacacggtc gaggtcaacc agaacttttc
     6121 cagatcgtcg aaaaaagcga ttcgctcctg gcctggatga gttatttcga ggcccgcaag
     6181 gggaagctcg cgctcttcac cgatctcgtt tggctcgatc tcggtttccc cggcaagatc
     6241 gatgtgcgcg gaagtccgtt cgcacggttt ccacgggtgg tggtgagcgt cgacggcaag
     6301 gctcagctcg actacgaatc gatcatcgtt cagccgggtg tcgcttatga agtcgcgcgc
     6361 tggcaaagcg cgccgggcag ctataccgcc ctcgaccttc tgggcggcgc gcgctattgg
     6421 aatcaggacg ttagtctctc gcttcgcctg accggcacgc tcgaagttga tcttgagcga
     6481 ctcgggctca agttcaaacg ctcgcgccgc gtggccattg cccgggccga cgacctcgaa
     6541 tgggtcgatc ccgtggtcgg cgcgcgcgtc aggcatcaga tcgctcccgg caaggagctg
     6601 cggctcgaag gcgatatcgg cggctttggc gtaggcagcg aattcgcatg gcaggccgtg
     6661 ggcgtctaca gcttccatac gaagtgtctg ggcgtgccac tccacgccga tatcggctac
     6721 cgcgcgcttt acgttgacta tagcgaggac ggccggttcg gaaagcaagg tcttgatctc
     6781 gtgcagcatg ggccagtgat gggcgtaacc ttcaactggt agtgcgtcag tttgtagtgt
     6841 cagtctggga ggttagccat gaaggtgctc attgccgcgg ccagagggat atgcgctgtc
     6901 tttgtctcgc tactgttcat tgacgaatcg cgatcaggtc cggttccgct cgaaagcgac
     6961 gccggagtca tctcaatggt cgccacggtc gaggccatcg acgtcaccaa ccagctggtt
     7021 acggtcgtcg gaccgaacaa caactgggtc gtcgtcaagg tcgggcccga acacatcaag
     7081 ctggtcaagg tcaaggagcg catcaccatc agctacgccg atgaagtggc ggtagcgctg
     7141 agaaagtatg ccggaccgcc gaaggcgacc gagaacccga tcgccaaaga cgagctggcg
     7201 gacatgaaca tgaatccccc gaccatggcc gagcaggatt gggtcgaggc cacgccgcat
     7261 ggcgccaagg agctcaccac gatcgagatt tccgacacgg tcgccgcggt gaaccggaac
     7321 cggcgcacca tcactttctt cggaacgggc ggcaagacgc gcacgatcgc gatcgctccg
     7381 aacgttccgg gtttcgagca ggtcgaggtc ggcgaccgga tcgtgctgct cgtgacgcgg
     7441 gccgtggcgg tcgatatcag gcccgtctaa tcgcgacgaa ttctctcgcg gcgcgcgatg
     7501 gctttgcctc gcgcgtcgct tttcgtcgtg tggcgcggct cagatttcct gcgcttagcg
     7561 ctcccggccc acgacctcgt agccgaactc tccggcagac gcggtgagcc agcgccagaa
     7621 gctcgcggag acgctacgga aaatcgcaag gtcgtaggtc ggtgcgtcgt cgacctgcca
     7681 gagctgtacg cccatgagcg agatcgtgct ggtggcggcg cttccgggcg tgaacgcgcg
     7741 agggtgaagg tcgatcggca ggcccttggc gagcacggcg cgggcacgcg ggccgctcac
     7801 ccgcagcacc gccctgccgc cgctctgatc cgagatcgag gcaagcccgc gcaggcgttg
     7861 tccgagatcg cgggcgagcg tctcgttgac gagcgactcc gacacggcga gccattgtcc
     7921 cggcccgtag ccgacgaagc ttattgaggg accatgcgcg acgcgcgagc catcgggaag
     7981 ctcgactccg taggccgaag caaccgctcc tttcaaggct tcggcctgcc ccttgcgggc
     8041 cgcgaccgtg gcgagaccaa gcccgacccg ttcctcgatc gtcacgccgg gatcgcctct
     8101 tccctccccg aggcgggtgg gaacggcaag gccggcgagc gcggagacgg cgtggaacgg
     8161 acgcttagcc acggacgcgt tccccctcgg gatcgatgaa acagggcggc cgcacctcga
     8221 ccagaatatc gccgccacgc acatggtcgt aggcgcgcac gatctcgccg tgccgctccg
     8281 ggccgcgact caagagacca aggccgagcc aatggccgag cgacggcgag aacgcgatcg
     8341 aggtgacata gccctggtcg ttcctggccg tcgcctcctt gtccttgggg atgagatggg
     8401 cgccggcgcg cacgcggtcc tcgcgggcga gcggcctcag gcccacgagc gtcggccgca
     8461 aaggatcggt caacgcttcg cgggccgcca tgaaccggcc gatgaagtct ttcttggtcg
     8521 agagcatctt cccgaacccg aggtcgtgcg ccgtggtctg gccattcaac tcgcctccgg
     8581 ccgggtggcc tttctcgatg cggagcacac cgagcgcctc ggtgccgtag ggcgccaacc
     8641 cgaagggcgc gccggcctcc atgatggcgc ggatcagcgc ctcgccgaac cgcgccggca
     8701 cgccgatctc gtaagcaagc tcgccggaga aggagagacg gtagagccgg gccggaacgc
     8761 cgcccgccac ggtaacctcg ccgcaggcaa gatagggaaa ggccgcgttt gagagatcgt
     8821 aggcgggatc gaccacccct tgcagcacgt cgcgggcgcg gggacccgcg acggcgaact
     8881 gggcccactg ctccgtgacc gagaccgtct gcacatcgag ctcgggccac aaagcctgct
     8941 ggcagaattc gagatgctgc atgaccttca ccgcgttcac ggtggtcgtg gtcatgaaat
     9001 agtggtcctc tgcgagccgc gcggtcgtgc cgtcgtcgag cacgaagccg tcctcgcgca
     9061 gcatcagccc gtaacgcgcc tttcccaccg gaagcgtcga gaaggtgttg gcgtagacgc
     9121 gatcaagaaa gatgctggcg tccttgccct gcacgtcgat cttgccgagg gtcgagacgt
     9181 cgcacacccc agcctggccg cgcaccgtcg ccacctcgcg gttgaccgaa tcctgaggct
     9241 cctcgcccgg cttcgggtag tagagcgcgc gaagccaggg gccggtctcg acgaacacgg
     9301 ccccctgctc ctcggcaaag ccgtgcgacg ccgcaagccg cgcggggcgg aaatcggggc
     9361 cgcggtgatg gccggcaaag gcgctgatgg cgaccggtgt gtatggcggg cggaaggtgg
     9421 tggtgccgac cgcgccgata tagcgccccg tgacggcggc gagcacggcc gcgccattgg
     9481 cattgccggt cttgccctgg tcggtcgcca tgccgagcgt ggtgtagcgc ttgagatgct
     9541 cggggatgcg gaagccttcg cacgcggcga gcttcacgtc cgacaccgcc acgtcgttct
     9601 ggaaatcgat gaaggctttg cctttgcttt ctgccacctg ccacagcggg ctcgccgtga
     9661 cgctctcatc ggaagcacgc gggcgcgaag cggaagcggc gaagccgcat tccgaggcgg
     9721 ccttggcgcc ggcttcctcg ccttcggcga gacatgcggc aagcccgtac gtgcctcgtg
     9781 ccgcgccggc gacgctcatg cctggcggca gggctccggg cacgagtgcg gcgagcctgt
     9841 cgttccattg cggcttggcg ttgagatggg tggtgagatg gagcgtgggg ttccagccgc
     9901 cggagacggc aatgagatcg caagccatgc gctcgcggcc tttttcgtct ctgatctcga
     9961 cgcctttgac gccatgaagc ccgcgcaccc gctcgatcac ggcgacggcg cgatagcgtg
    10021 ctcccgccgc ctccgccgct tcttgcgcgg cgcgtgacgg ggtccgccgc ccatcgaccg
    10081 cagccgcaat ctcgattccg gcctgggcga ggtccgatgc cgtgcggaac gcgtcgtcat
    10141 tgtcggcgaa gataacggcg cgccgtcccg gcgcgaggcc gaagcggttg acgtagctcc
    10201 gcacggctcc cgccagcatg accccgggac ggtcgttgtt gccgaacacg atggggcgct
    10261 cgagggcgcc tgcgcagacg accacgcgct tcgccacgat gcgccacagc ctctggcgcg
    10321 gctcgtgcgc cagcggtttc aggacatggt cgttgacccg ttcgagcgcg gcataggtgc
    10381 cgccgtcgta gacgccctgc accgtcgtcc gccgcaggat gcggcactcg ggttgcgctt
    10441 cgagctcggc ctcgaccgtc tcgacccaat cgcgcgcagg tttgccgtcg atttcgtagc
    10501 gctcggcaag gagccgcccg ccgagacgga aatcctcctc gcaaaggatc acccgcgcgc
    10561 cggaccgcgc ggcggcaagc gtggccgcaa gtcctgcggg gcctgagccg acgaccagca
    10621 catcgcagaa ggcgtaagcc ttctcgtaat ggtcggggtc cgccacgcca gccccgcggc
    10681 cgagacccgc ggcgcggcgg atcatcggct catagacctt ctcccagaag gtgggcggcc
    10741 acatgaaggt cttgtagtag aagcccgcgc tgaagatcgg gccgaagagc tggttcgccg
    10801 ccagcacatc gaatttgacc gacggccacc gattctggct cgccgccgag agtccgtcgt
    10861 aaagctcgat cattgtggcg cgggtattgg gttcgcgccg cgcgcccgtg cgcagctcga
    10921 cgagcgcgct cggctcctcg gagcccgcgg tgaggatgcc gcgcgggcga tgatatttga
    10981 acgagcggcc gaccaggcgg atgccgtttg cgagcagcgc cgaggcgagc gtatcgccgg
    11041 ggtgaccggc aagcggcgcc ccgtcgaact cgaagcgcac cgtctgcgac cggtcgatca
    11101 ggccgccttc gccgagacga ttcggctgcc gtgcggtcgt catttggaag cccccttctc
    11161 ggcgaactcc accgccacga tctcgtgcgt gctcatgttg cgggtcacca caagccaggc
    11221 atggcagcct gcgctgtgat gccagtattc cttgtgcagc cctgccgggt tgtcgcggag
    11281 atagacgtag tcgacccagg cttgagccgc gccgggatcg agactgtcgg gccgcctcac
    11341 cgtggcgtcg ccgcgatagg tgaattcttc gagcgcccgc tcaccgcaat aaggacacct
    11401 gatgcgcatc ggctcggcct cagtgcaggt tcggttgcgc gcccatgccc ttctcgtcga
    11461 tgacccggcc ttgggcgaag cggtcgagac gataggcggc ggcgagcgga tgcggctcgc
    11521 ccgtggcgat gagatgagcg aagcaccagc ccgatgccgg cgtcgccttg aagccgccat
    11581 agcaccagcc ggcgttgaga tagagcccgt cgatcggcgt cgtgtcgatg atcggcgagc
    11641 cgtccatgct catgtcgagc agtccgcccc aggagcgcag gatgcgcagg cgtccgatca
    11701 ccggcatgag cgccatgccg ccctcgcata cgtcctcgat caccggcaaa ttgccgcgct
    11761 gggcatagga attgtagccc tcgatatcgc cgccgaagac gaggcctcct ttatccgact
    11821 ggctcacgta gaaatgcccg gcgccgaacg tcatcaccac gtcgatcacg ggcttgacgc
    11881 cctcggtgac gaaggcctgg accgagtggc tttcgatcgg aagccgcaaa cccgccatgg
    11941 cggcgacgcg cgaggaattg ccggcgcaag cgagcgccac cttcttggcg gcgatgaagc
    12001 ccttttccgt ctccaccccg cgcacacggc cgccttcgat gcggaagccc gttgccgcgc
    12061 aattctggat gaggtcgacg ccacgggaat cggcggcgcg ggcatagccc caggcgacgg
    12121 cgtcgtggcg gacggtgccg ccgcgcggct gcaacaggcc gccccgcacg gggaagcgcg
    12181 cattgtcgaa atcgacgaag gggacgagcg cccgcacacc ttcgcggtcg agcagctcgg
    12241 catcgacgcc gtgcagacgc atagcgttgc cgcgccgcgc ataggcgtcg cgctgcgggt
    12301 cggagtggaa gacattcagc acgccgcgct ggctgaccat ggtgttgtag ttgaggtcgt
    12361 gctcgagtcc ctcccacagc ttcatcgacc attcgtagaa gggaagattg cccggaagca
    12421 gatagttgga gcggacgatg gtggtgttgc gcccgacatt gcccgagccg agatacgatt
    12481 tctcgatcac ggcgacattg gtgatgccgt gcttcttggc gagataatgg gccgtggcga
    12541 gcccatgccc gccaccgccg acgatcacca cgtcatagga aggcttcagc tccggctcgc
    12601 gccagagcgg cttccaatag cggttgccgg tgcaggcatt ggcgaggagg ctcgcgaccg
    12661 aatagcgcat ggccacgatt gtgcgccgag gcgcacgctt cgattgacat ttcacgccgc
    12721 ctgcttgaca ttttgcgaca tgagcagaaa gcaagccgca ccccaggcgg cagaagaggg
    12781 cacgatccct gtcaagcagc ctgcggcgcg gaccgtcggg ttcctgctcg tgccgggttt
    12841 tgcgctgatg tcctatgccg cggcgatcga gccgctccgc gccgtcaatc ttctctccgg
    12901 ccgggagctt taccgctggt ggcatgcggc gcccggcggc aagcctgtgc ttgcctcgaa
    12961 cggtgtcgcc atcatccccg attgcggcac caataccgag cgcgcggccg acatggtctt
    13021 tgtctgcgcc ggcggcaatc cggcgctgtt cgaggaccgg tccgtgtttg cctggctccg
    13081 ccgcatggcc cgcaagggtg tcgccatcgg cggcatttcc ggcgggccct atgttctcgc
    13141 caaggccggg cttctcgatg agcgccgcgc cacgctccat tgggagcatc tgcccgcctt
    13201 ccgtgaggcc tttcctgatg tcgcggtggt gccgtcgctg ttcgagatcg acggcaaccg
    13261 tatcacctgc tcgggcggca tctccgcgct cgacatgatg gtcgccatca tcgagaagga
    13321 gcacggccgg cagctcgcag ccgccgtcgg cgactggttc ctgcacaccc atgttcgcga
    13381 aggcttcggc ccgcagcgca tggatcttcg ctaccgcctc ggcattgctg acgagaaatt
    13441 gctcgccgtg ctgcgcgcga tggaagcgag catcgagacg ccgctgtccc gtttctcact
    13501 tgcgcgtgag gccggcatct cgctcaggca actcgaacgg ctgttccgcc gccatatcgg
    13561 ccatgggatc catcggcact atcggtggct ccggttggag cgcgcgcgcc agctcctgcg
    13621 cgagacgact ctgcccgtgc ttgacgtggc gcttgcgacg ggcttcgcct ctgccagcca
    13681 gttcgcccga gcctacgcgg gtgccttcgg cgagcccccg agccgcacgc gcatcagcgg
    13741 gtagagagag cttattgtgc ttattgagac gcgaccccga cgaagggggt gccgcccgga
    13801 atgcaggccc actggcctac ggccggcgtc ctgaccaggg tcttgttctg ctcgagatac
    13861 tgcatcgaca cgaggtagcc ggcgggccct atgcattggc gatagctcaa ttcctgcgcg
    13921 atccattccg gcgggattgg aacctcgatg cagccgatct cgcgaaaata ggcgttccgc
    13981 tcctcgcgcg tcatcccgcg ctcattctcg taactcttgc cgtggttcgt cggctcgagc
    14041 ggagtctcgg cggcggggca agccgtgacg acgacctgga cgtagggcgg attggtatcg
    14101 atccgcaccg gccgttcctc ggcaagcgcg ctggccggca acagaaggag gagaagagcg
    14161 aggggacgca tggccgtcat cctctgggcg ggtaggcgcc gtcgaaagcg tgctgctcgc
    14221 gccagcgcgg tcccgggccc cgcatgtagt gacgctcggg gcggtaggga tcgaacaggc
    14281 cgttcacgac cttgcgcaga gcgccggcaa cgaccacggg attccaggtc ttggggcttt
    14341 cagtgacgct cgcggtcact gcgctcatct gtaagtctcc gacttgcatc aacatggctg
    14401 tcactccagt ggacatctct tcatgctcaa agctaagcct ctgaggcggc cttgggccaa
    14461 tatccaaagg ctatcgtttc cgaagccgca ccgtatccga cgcatccggg gccaaagagg
    14521 ttcaaagcct ccccttggga cgtggttccg gtttgcccga tcgtgctagc ttcgaggcct
    14581 ggaaacctgc gacgattgct gatgttgtgc tcaaaggcct gaaaggaggc ggcatggcga
    14641 ccacgagact ttcgtccaag gggctgaagt cgcgcctccc tgtgcgccgc cgcacc
//
LOCUS       DASUQP010000217        19581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_106897,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000217 DASUQP010000000
VERSION     DASUQP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 19581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19581
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106897"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..788
                     /locus_tag="VFY74_09815"
     CDS             228..788
                     /locus_tag="VFY74_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEX5932029.1"
                     /translation="MTKIPFWPMAVTAVVSAVLGGSAISAQDKYTVQVPGGLAFSEFK
                     GYEDWQTVAVSQTETVINAILANPVTIDAYRAGVPGNGKSFPDGSKMVKIHWKQKKST
                     EAPDPTTVVPDTLQSVGFMVRDSKRFADSGGWGWGEFKYDAASDTFSPYTLADNPPQG
                     NDAKCGFACHTIVKAKDYVFTAYPKR"
     gene            complement(1047..2078)
                     /locus_tag="VFY74_09820"
     CDS             complement(1047..2078)
                     /locus_tag="VFY74_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX5932030.1"
                     /translation="MAKRNPFAAIAHGHDLAEAIVDAIREPLLVLNPDLRVIAASRSF
                     YRTFAVTPRKTEGRLVFELGDGQWNIPSLRALLEDIIPKRRTVESYEVEHEFPSIGRR
                     VMLLNARRVFDEDGSASAILLAMEDVTRRRETDREKDELLQAKEILLQEMQHRVANSL
                     QIIASILLLKARTVQSEETRLHLHDAHQRVMSVATVQQQLQASALNERIQIEPYLTKL
                     CDSLAASMVGERRALSIKVQASSGGAVSSEAISIGLIVTELVINALKHGFPAGAEGEI
                     LVSYDAHDSGWALSVADNGSGTQADGPAHTGLGTSIVEALAHQLNATVQKTSGPQGTI
                     VSIIAPAVC"
     gene            complement(2226..2576)
                     /locus_tag="VFY74_09825"
     CDS             complement(2226..2576)
                     /locus_tag="VFY74_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX5932031.1"
                     /translation="MKLATFTAVAVLFSAGYAFAGAGAPVPEGSQSGEPAGRPSAVLD
                     DAACQDVWKMASPNGDTLAGDKAAPYIVNFQMVDTDKDGKISAEEFKTGCGKGWVQNA
                     DASTTKEMTPAQKL"
     gene            complement(2737..3042)
                     /locus_tag="VFY74_09830"
     CDS             complement(2737..3042)
                     /locus_tag="VFY74_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932032.1"
                     /translation="MAVDKVRIAFGPEAMSRFGQIIEEVSLNLIDDGLTAELVRSDEM
                     RRKLAQSLLGFASHWWTDTQIKQLLLRVLRNQISASRRHQTAAGNGMGTGTNCLRDS"
     gene            complement(3071..3157)
                     /locus_tag="VFY74_09835"
     CDS             complement(3071..3157)
                     /locus_tag="VFY74_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX5932033.1"
                     /translation="MDNTTLLIIIILVLLILGGGWYGRGRWF"
     gene            complement(3418..3621)
                     /locus_tag="VFY74_09840"
     CDS             complement(3418..3621)
                     /locus_tag="VFY74_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932034.1"
                     /translation="MFIPSLPELTIGWGVAYKGKESVVQCPSEPNIIVAMTSTETRKG
                     EVGVSKFIPSHLICLFFDEPFFA"
     gene            complement(3867..5546)
                     /locus_tag="VFY74_09845"
     CDS             complement(3867..5546)
                     /locus_tag="VFY74_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5932035.1"
                     /translation="MSLIETRGHQLFPVLDAGQIETAKRLASGPPRDFAAGTVVFDVG
                     ARHVPVWLVLKGSIDVVRRDGLNHESAITSLGPGQFSGEVSQLAGATTLASGRAGPEG
                     CTALPFDAAHVRALLIASAEVGEIMMRAFILRRVGLIEDGGAGSILVGKAGMPDLVRL
                     QGFLRRNGYPYTVLDATNDADGQAVVERFGVLPEQLPLMVCPNGTVLHRPTNSEAAVC
                     LGMLPERDPEKTYDVAVVGAGPAGLAAAVYAGSEGLSVLVLDQRAFGGQAGASARIEN
                     YLGFPTGISGMGLAGRAFNQALKFGVEIAIPLEAARLDCGSRNGMGAGLRRLELSDGS
                     RVKARTVVIASGVRYRRPPIPNLADFEGAGVSYWATAIEAKLCEGEDVALVGGGNSAG
                     QAVVYLAPRVNRLHLVVRGNGLETSMSRYLIDRIAALPNVELHTRTEIVGLEGDKVQG
                     LTAVIFRDRDSGATHSCSLRHLFLFIGADPNATWLDHCVAVDKKGFVMTGADVPQAPG
                     ATSRAILPLETSQQGVFAIGDVRAGSTKRVAAAVGEGAAVVAQIHSVLAMT"
     gene            complement(5706..7886)
                     /locus_tag="VFY74_09850"
     CDS             complement(5706..7886)
                     /locus_tag="VFY74_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5932036.1"
                     /translation="MPRVPANRFKAESDGVSRRTFLAASLAAGGGLLLSMKLSLPARG
                     AAPAISREVIGAFIRIDRDGRVHLTMPYIEMGQGTYTSISMLIAEELEVDLDQVQYEH
                     APPNDKFYANPLLGFQATGGSTAVRASWEPLRKAGAAARMMLVAAAAEEWKVDPRSCR
                     AEKGEVIHGESGRRLSYGALAEKAAALPVPSEIPLKERKNFRLIGKPVKRLDTPAKVD
                     GTALFGIDAKVPGMKIATVAACPVFGGKLKSVDDGRAMAVNGVSQVVHLEDAVAVVAQ
                     HMGAAKKGLAALDIQWDEGSHATLTTADIVGQLEAASQKRGAVARNTGNVEEALADAK
                     TKLEAIYQLPFLAHTTMEPMNCTVHVRKDGCEIWVGTQVMTRAQSTAASITGLPPERV
                     TVHNHLLGGGFGRRLEVDFIAQAVKIAKQVEGPVKVIWTREEDIQHDMYRPYFFDRLT
                     AGLDAQGLPVAWHHRICGSSVIARWAPPAFKDDLDFDTIEGAVEPPYAFPNMRLEYVR
                     HEPPGIPTAFWRSVGPSHNIFVVESFIDELAAAAKKDPVEYRLALLDKAPRAKAVLQL
                     AAEKAGWGQSLPNASGRGVSVQNVFGTFMAQVAEVNVSKQGEVHVERVVCAVDCGITV
                     NPDTIQAQIQSAIIYGLSAALHGEITVKNGRVEQSNFDNYRALHINEVPAIDVHIVDS
                     DEAPGGIGEPGTSALFPAVTNAIFAATGVRLRKLPVNQALLKAE"
     gene            complement(7890..8369)
                     /locus_tag="VFY74_09855"
     CDS             complement(7890..8369)
                     /locus_tag="VFY74_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5932037.1"
                     /translation="MAFTIKVNGVSHSVDVDSDTPLLWVLRDVLGMTGTKFGCGRALC
                     GACTVHLDGNPVRSCVMPIESVGEAPITTIEAIGESPVGRSVQSAWLDLEVVQCGYCQ
                     PGQIMSATALLSANANPNDEDINNAMSGNICRCGTYLRIREAIKVAAKSLSSDIEKG"
     gene            8461..9075
                     /locus_tag="VFY74_09860"
     CDS             8461..9075
                     /locus_tag="VFY74_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Isoquinoline 1-oxidoreductase subunit"
                     /protein_id="HEX5932038.1"
                     /translation="MKIVSGTLAAAFFAVLTIAVVCAEQPSAERQALKPPEDFAQIAD
                     QNERSLALFGEAAKVITHPRCVNCHPAGDRPSQGEDSHPHLPLVVRGPAGFGAVAMQC
                     VNCHGAKNYDAARIPGHPKWHLAPIEMAWQGKTLGEICEQIKDPKRNGGMTLEDLVHH
                     MAEDSLVGWGWNPGAGREPAPGTQKEFGALVEAWVASGAACPLP"
     gene            complement(9554..10021)
                     /locus_tag="VFY74_09865"
     CDS             complement(9554..10021)
                     /locus_tag="VFY74_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932039.1"
                     /translation="MALRILSGMIFVPNGGTARGIVVISFNPFQLSARSNPAFELRKE
                     QEIGPRERFLRRPVTMVAARQFAILALSSNPRVKLNDTVTRDSITISWNTSASTARID
                     QEEIPFLVIGEVPDMVPISKPTRGVTVKHPSDSLRGRASGRSKRKPSKRRKSG"
     gene            complement(10153..11253)
                     /gene="ada"
                     /locus_tag="VFY74_09870"
     CDS             complement(10153..11253)
                     /gene="ada"
                     /locus_tag="VFY74_09870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEX5932040.1"
                     /translation="MAAILAHPAVANGARSPALEEARWLAVLARDAAFDGRFFYSVAT
                     TGIYCRPSCAARRPNRAHVRFHRTAAEAERAGFRPCKRCKPNEPSLIQEHAAKVRVAC
                     RMIEEAEEAPRLGDLAAAVGLSPYHFHRIFKSMLGITPRGYAAAQRRKRVRGELGRSA
                     TITEAIYGAGFNSSSRFYATSSEALGMTPGQFRKGAPDADIKFAIGKCSLGLVLVAAS
                     AKGVCAILFGSDPVSLRGDLQRQFPRARLIEGDPSFHRVTAEVIAYVENPREDLNLPL
                     DIRGTAFQHRVWEALRRIPPGATASYAEIAKAIGAPRAVRAVARACASNRIAVAIPCH
                     RVVASDGSLSGYRGGVERKRALLAKEAKSLQK"
     gene            11530..12897
                     /locus_tag="VFY74_09875"
     CDS             11530..12897
                     /locus_tag="VFY74_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5932041.1"
                     /translation="MSDRAEPGFINSGLLGRIGQAVLRPFREIAARLLATLWLIVAVG
                     VIMVSLVLIAGLSKKFALIGFVSFVASAMLLPRHERDQALPSALGPASPSVPAVAAMR
                     VLAEALPDPVILLSPGGHVLFCNAQARSLFASLREGSHISSVIRTPEFLDAVAAAPGL
                     GRPVTVTYAERVPVGRRMAATVAPLAGRGEEAGLANILVLLRDLTEIERINQMRADFI
                     ANASHELRTPLASLRGFIETLQGRAKGDPAARDRFLPIMAEQASRMTRLIDALLSLSR
                     VEMNAHVPPSDLVDLNDVLGHAKDTLEPLARDMGFTFEVGRFPRPAIVRGDRDELMQV
                     VQNLAHNAFKYGVKGGTVRIEAKHIPALGRQTGRYTIAVIDNGPGIAPEHLPRLTERF
                     YRVDVAASREKGGTGLGLAIVKHILNRHRGELAIASVPGKGSTFTVMLNEVRAHSPER
                     GAAAG"
     gene            13583..14641
                     /locus_tag="VFY74_09880"
     CDS             13583..14641
                     /locus_tag="VFY74_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5932042.1"
                     /translation="MNRIGIVASTLAVAALCTTGPALARDQLKVVGSSTVFPYSQAVA
                     EDFGKKTGNEAPVVESTGTGGGMKIFCQGVGEGNPDVTGASRAMKKSEWELCAKNGVN
                     DITEIQIGYDGLSIAQSKKGNPIDLSKQQLFLALASEIPDGDKLVANPNKKWSDVDKS
                     LPAVAITVYGPPPTSGTRDAFVELAMHEGCKSLDYFKKKKGELDKDGFDKLIKEKCTP
                     MRQDGPFIEAGENDNLIVQRIEADPNALGIFGYSFLYENQDKLQGVKIGGVQPTFDTI
                     ADGSYGLSRPLFIYIKNAHRKVIPGMNEFIAEYTSSEAMGPDGYLHERGLVVLSDDML
                     KEMQDRAKSADKMAAPTS"
     gene            14769..16148
                     /gene="pstC"
                     /locus_tag="VFY74_09885"
     CDS             14769..16148
                     /gene="pstC"
                     /locus_tag="VFY74_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5932043.1"
                     /translation="MIGYVLLGIIALSLFSYLLGRRRAMASVGGRQQELHSRPSYHGA
                     FVAAWVGIPSILLVLVWLVLQGPVIDRMLLWSLPPGATDGLTSGQIDLMLSEIRSVAA
                     GNIFSEPSLVIQEAAERYQRWKAIARYAMVVVALSIALFGLFVARARINPEFRARRAT
                     ERVMSGLMMACSLIAILTTLGIVLSLLFEALRFFDRVSPLEFFFGLNWEPQIPIREGQ
                     VTAGGSFGAIPVFTGTLLISAIALAVAIPIGLLSAIYLTEYAHPKFRAVVKPMLELLA
                     GIPTVVFGFFAVLVVAPSMRDFGAMIGIPIAPNSALAAGAVMGIMIIPFVSSLSDDAI
                     AAVPRSMRDGSYAIGATKGETITKVLLPAALPGIMGGILLAASRAIGETMIVVMAAGI
                     IASLTLNPLEPVTTVTVQIVTLLIGDVEFDNPKTLAAFALGLVLFVVTLALNVLALHI
                     VRKYREQYE"
     gene            16213..17790
                     /gene="pstA"
                     /locus_tag="VFY74_09890"
     CDS             16213..17790
                     /gene="pstA"
                     /locus_tag="VFY74_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5932044.1"
                     /translation="MKRRYAADRRLQAYGIIAIALAVGLLGVLITSLIVNGLPAFVQT
                     KADLAVFIDAAKVDAENPAGGNYRALVREALEPFVPADATDKQKTDAAKILTSDAPYI
                     VRDYILKHPEAIGKTVALPIPLSDPFDQLNKGVIPMDVDALSWSQVRYFERLRNRGVI
                     VEDDGRTGLKLDVYVDPAKIELGPPPSGDFATLARDGLQAYLPDVHDTALFAMLADDA
                     PEAISARVAADPALIGKTIQVILPVSPAVTALAEGKSPKILNPRFKADQIETFEALKA
                     KGLVHAPFNWELFFNADSRFPERAGLAGAITGTFLMLLVCFGLSFPIGIAAAVYLEEF
                     APKNRLTDLIEVNINNLAAVPSVVFGLLGLAVFLGFFGLPRSTPLVGGMVLSLVTLPT
                     IIIATRAALKAVPPSIREAALGVGASKHQVVLHHVLPLAMPGILTGTIIGMAHALGET
                     APLLLIGMNAFIPSVESMGVLEPATTLPTQIFSWADSPERGFVSRTSAAILVLLAFLV
                     VMNLLAIVLRQKFERKW"
     gene            17877..18653
                     /gene="pstB"
                     /locus_tag="VFY74_09895"
     CDS             17877..18653
                     /gene="pstB"
                     /locus_tag="VFY74_09895"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5932045.1"
                     /translation="MLPAAERELKMTARDVNVWYGEKQALFDVSLDIFDRSVTALIGP
                     SGCGKSTFLRALDRMNDVIEGCRMTGEITLDGEDIYSPEVDPVQLRARVGMVFQKPNP
                     FPKSIFENVAYGPRIHGLAGSKAELEEIVIKSLTRAGLFEEVKDRLNESGTGLSGGQQ
                     QRLCIARAISVNPEVLLMDEPCSALDPIATARIEALMEELKESLCIVIVTHSMQQAAR
                     VSQRTAFFHLGKLVEVDDTVRMFTNPADTRTQDYVTGRFG"
     gene            18675..19409
                     /gene="phoU"
                     /locus_tag="VFY74_09900"
     CDS             18675..19409
                     /gene="phoU"
                     /locus_tag="VFY74_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5932046.1"
                     /translation="MMDKQHEHIVRSYEEELELLNNKIAKMGGLAEQVLGQSIDALER
                     RDPELAASTINEDEAIDALEREIEEQAVVMIARRQPMAYDLRQIMAALRIAGDLERIG
                     DLGKNIAKRAVAVVSEQQPKQLMLGLKHMGELALAQLKEVLDAFIERDADRALKVWYK
                     DEEIDAMYNSLFRELLTYMMEDPRNIGLCTHLLFGAKNIERIGDHATNIAETVYYLVH
                     GAAITDQRPKGDTTSSTPVPLKRSAG"
BASE COUNT         3697 a         6165 c         5957 g         3762 t
ORIGIN      
        1 gaaaaccatg acgcgcgctg gcagggctat ctcaacgacc tcgcggccaa gggcctctcg
       61 cgctgagcgc ccgtcgaggt ccccgttcaa gtttcagtga acgccctggt ttatgacagt
      121 gtgtgtgaaa ccatgccaac agacaagaac ggcccctcgc actgcaccaa cgccgtttga
      181 acggacgcca gtgaacttga cttgcaacgc tagcgcagga gtaagccatg acgaaaattc
      241 cattctggcc gatggcggtc actgctgtcg tgtctgccgt cttgggcggc agcgccattt
      301 ccgcgcagga taagtacacc gtgcaagtgc cgggcggcct cgcgttctct gagttcaagg
      361 gatacgaaga ctggcaaact gtcgccgtca gtcagacgga gactgtgatc aatgcgatcc
      421 tcgccaatcc cgtaacgatc gacgcctacc gggccggggt tcccggcaat ggcaagtctt
      481 tccccgacgg ctccaagatg gtgaagatcc attggaagca gaaaaagagt acggaggccc
      541 ccgatccgac gacggtggtg ccggataccc tgcaatccgt tggtttcatg gtgagggata
      601 gcaagaggtt cgcggacagc ggcggatggg gatggggcga gttcaagtat gacgccgcgt
      661 ccgacacgtt ctcgccctac accttagctg acaacccgcc acaggggaac gacgccaaat
      721 gcgggttcgc gtgccatacg atcgtgaagg caaaggatta cgttttcacg gcgtacccga
      781 aaaggtgacg gggatcgtca tcattaccgt cggttcctac aatgcttcct acatcacagg
      841 cgtcgaactc gccgtcgcga ccgcggccgt actcagccat agccgggcgt cattagggcg
      901 gaccaccgac tcccgcgggc gtcgcgccag caaccggaac gaactgctcg acgatgtccg
      961 ctagtggcac ttagcggaaa tcacatcgtc gctaattaag gtctgcttct gaactaaagc
     1021 ggacatggcg gcgtgtgcat tggcttctaa caaacggcag gagcgataat tgagacaatg
     1081 gtgccctgag gaccacttgt cttctggacc gtggcgttga gctgatgggc caatgcctcg
     1141 acgatgctcg tgccgaggcc ggtatgtgcg gggccgtccg cttgggtccc tgatccattg
     1201 tcggcaaccg agagcgccca gccagaatca tgcgcgtcgt agctgaccag gatttccccc
     1261 tccgctccgg ccgggaagcc gtgcttcaag gcgttgatca ccaactctgt cacgatcaac
     1321 ccaatgctaa tagcttcgct ggatactgcc ccgccagagc ttgcttgcac cttgattgaa
     1381 agcgcgcggc gttcaccaac cattgaggct gcaagactgt cgcacagttt cgtcagatag
     1441 ggctcgatct ggatcctctc gttcaacgca gaggcctgca gctgttgttg caccgttgcc
     1501 actgacatga cgcgctggtg ggcatcgtgg aggtggagac gcgtctcctc cgattgcacc
     1561 gtccttgcct tgagcagaag aatgctggcg ataatttgaa gactgttggc aacgcggtgc
     1621 tgcatctcct ggaggagaat ttccttggct tgaagcagct cgtccttttc acgatcagtc
     1681 tcgcgacgcc gagtcacgtc ctccatcgcc agaagaatgg cggaggccga accatcttcg
     1741 tcaaacaccc tgcgggcatt gagcagcatc acgcgccggc cgatcgacgg aaattcgtgc
     1801 tcgacctcat aggattccac ggtgcggcgc ttcgggatga tgtcttcgag cagcgcgcgg
     1861 aggctcggaa tgttccactg cccgtcgccc aactcgaaga ccaggcggcc ttccgtcttt
     1921 cgtggcgtca cggcgaaagt ccggtagaac gagcggctcg cggcgattac gcgtaagtca
     1981 gggttgagca cgagaagagg ctcccgaatg gcgtcgacaa ttgcctcggc caagtcatgt
     2041 ccatgcgcga tggcggcgaa aggattgcgt ttagccacca gaggattcca ctttttgctc
     2101 tatcttcgat gtgattttag caaacctggc ggcgggatcg agcccgccgg atcaccaaaa
     2161 ggaaacggcc ccaggaaacc ccggggccgc ctcatcacgc gccgttacac tacgcggcgc
     2221 taaaattaga gtttttgcgc cggggtcatt tccttggtcg ttgaagcgtc ggcgttctgg
     2281 acccaaccct tcccgcagcc ggtcttgaac tcttccgcgg aaatcttgcc gtccttgtcc
     2341 gtgtcgacca tctggaagtt cacgatatac ggcgctgctt tgtcgcctgc caaagtgtcg
     2401 ccattcggcg aggccatctt ccatacgtcc tgacacgcgg cgtcatccaa gacggcgctc
     2461 ggacgacccg ccggttcacc cgactgggaa ccctccggaa ctggagctcc agctccagcg
     2521 aatgcatatc cggcactaaa cagaaccgcc acggcggtaa aggttgcgag tttcattgca
     2581 tgctccaatt tgatttgatt ggtgggctcg gccgcgcggt tttggcgcgg aaaaggactg
     2641 cccggcaggt ctcaagagag gggaaacatc atcaaggagg gtgtcgatca aacggacaca
     2701 tcatcgagga cggtgtcgtc ggacccggtc atcagcctaa ctgtctcgta ggcagttagt
     2761 tcccgtcccc atcccgttcc ccgccgcggt ctggtgccgg cgggaagccg agatctggtt
     2821 gcgcagcacc ctcagcaaaa gctgcttgat ttgagtatct gtccaccagt ggctagcaaa
     2881 gccgagcaga ctttgagcca gttttctacg catttcgtcc gatctcacga gctcggcggt
     2941 caggccgtcg tcaatcagat tcaacgacac ctcttcaatg atctggccaa atctcgacat
     3001 cgcttccggg ccgaaggcga tgcgcacctt gtcgactgcc atttgtaacc ctcgatggaa
     3061 actacgcctg ctagaaccag cgtccgcgtc cataccagcc gccccctaaa atgagcagga
     3121 cgagaatgat gatgatgaga agtgtcgtgt tgtccatgtc ggtctcctaa ggtgaataaa
     3181 agaagcccgg ggggccgggc ttctctatat tacacttggt acagggcatc ggggctgacg
     3241 ggggccgggg gaattgcgcc aatgccacta agcggagaac ccgcccgcgc ggacttagtt
     3301 ccggttgccg cgcaatcggg ctcttttctc tgatctgaca gtcgtgttcg acctaccgca
     3361 gcagcgcgtc aatgcgcgca cgcgcctcaa tgagtgcctc cgtccatgcc gggtggatca
     3421 cgcgaaaaac ggctcgtcga agaataggca gatcaggtgc gacgggatga acttcgacac
     3481 tccaacctcg cccttgcgtg tttccgtcga tgtcatagcc acgataatgt tcggctcgct
     3541 gggacattgg accaccgatt ccttcccttt ataagcaacg ccccaaccga tggtcagttc
     3601 tgggagggag ggaataaaca tcgatgtcgg cttttggcac ttagcggaca cgctcaatgc
     3661 gccgacgaat gtccgcttct gggggcaaag cggacgttga ccaaccgctg cttaccgaga
     3721 ggcggaacac ggacccgacc ttgagcttcg gctcggtcac tatctgggcc tcgcaagcta
     3781 aaacgcctgc cggccgcgat gggggctgtc atcacctctc gctgggggtt gcgtcagccc
     3841 gaaccccacg atcgtgccga agtggactag gtcattgcga gcacgctatg aatctgcgcc
     3901 acgacggccg cgccttctcc gacagctgcc gcaacgcgtt tggtagagcc cgcacgcacg
     3961 tcgccgatgg cgaagacgcc ctgctggctc gtttcgagcg ggaggatggc tcgtgacgtt
     4021 gccccgggcg cttgcgggac atccgcccct gtcatcacaa agcccttctt gtctacggcg
     4081 acgcaatgat cgagccaggt ggcgttcgga tccgcgccga tgaacagaaa gaggtggcga
     4141 agtgaacagg aatgagtagc gccggagtcg cggtccctga atatgactgc ggtcaaaccc
     4201 tgaaccttgt caccctccaa acctacgatc tctgtgcgcg tatgcagctc cacattgggg
     4261 agtgcggcaa tgcggtcgat caaatagcga gacatcgagg tctccaagcc attccctcgt
     4321 accacgagat gcaaacggtt cactctcggg gcgagatata ccactgcttg tcccgcagaa
     4381 ttgccacctc ccacaagggc gacgtcttcg ccctcgcaca gctttgcctc gatcgcagtg
     4441 gcccaatagg aaacaccggc gccctcgaag tcggccaggt tcggaatggg cggacggcgg
     4501 tagcggacac cggaggcgat gaccacggtg cgagccttga ccctgctgcc gtcactcaac
     4561 tcgaggcgcc ttagccccgc gcccattccg tttctcgaac cacaatcaag acgcgccgct
     4621 tcaagcggaa tggcgatctc gacgccaaat ttgagggcct ggttgaacgc tcggccagcg
     4681 aggcccatcc cggaaatacc ggtgggaaag ccgagatagt tctcaatacg cgccgatgcg
     4741 ccggcctggc ctccaaaggc cctttgatcc aggacgagca cggaaagacc ttccgatcca
     4801 gcatagacgg cggccgcaag cccagcaggg cccgcaccga ccacagcgac atcgtaggtt
     4861 ttttcagggt cgcgttcggg aagcatgccg aggcaaacgg ccgcttctga attggtgggc
     4921 cggtgcagca cggtgccgtt cggacaaacc atcagtggca gctgctcggg aagaacaccg
     4981 aaacgttcca cgacagcttg accatcggca tcgttggtcg catccagcac ggtgtacgga
     5041 tagccgtttc gacgcaggaa cccctgaagc cttacgaggt ccggcatccc cgctttcccg
     5101 acgagaatcg agccggcacc gccgtcctca atcagtccca cccgccggag gataaacgcg
     5161 cgcatcatga tctcgcccac ctcagcggag gcaatcagca gggctcgaac atgggcggca
     5221 tcgaacggca acgcagtaca gccttcggga ccagcgcgcc ccgacgcaag cgtcgtggcc
     5281 ccggcaagct ggctgacctc gcccgagaac tgaccggggc caagactggt aattgcggat
     5341 tcgtgattga ggccgtcgcg acggaccaca tcgatcgagc ccttgagcac gagccagacg
     5401 ggtacatggc gcgcgcccac atcgaagacg accgttccgg cggcaaagtc gcgcgggggt
     5461 ccgctcgcca aacgcttcgc cgtctcgatc tgccccgcgt cgagcactgg aaacagctgg
     5521 tgaccacgcg tttcgatgag gctcatgaat tggctcgcga accgaagtgc actcttaagt
     5581 gcgctctttg tatcgatctg acagctggct ttgcaaacgc cttctgtttt ctgtcgggga
     5641 agatcgcgag cggcatcgcc gggccacacg acgacattcg gaaacgtcca tgtgcggagc
     5701 gggtattact cagccttcaa cagcgcttgg tttaccggaa gcttgcgaag cctaacgccg
     5761 gtggcggcaa agatagcgtt ggtgaccgca ggaaaaagag cggaggttcc cggctcgcca
     5821 atgcctccag gggcttcgtc gctgtcgacg atatggacgt cgatcgcagg cacttcattg
     5881 atgtgcaaag cgcggtaatt gtcgaagttg ctttgctcga cgcgcccgtt cttgaccgtg
     5941 atctcgccat gaagcgccgc cgacaatccg tagatgatgg cgctttggat ctgcgcctga
     6001 atcgtgtccg gattgacggt gatgccgcag tcgacggcgc acacgacacg ctcaacgtgg
     6061 acttcgccct gtttggaaac gttgacctct gcaacttgcg ccatgaaagt cccgaacacg
     6121 ttctgcaccg ataccccgcg gccggaggca ttcggcagcg actgtcccca tcccgccttc
     6181 tccgctgcaa gctggagcac ggccttggcg cgcggggcct tgtcgagcag ggcgagccga
     6241 tactctaccg gatccttctt ggccgccgct gcaagctcat cgatgaagct ctccaccacg
     6301 aagatgttgt ggctcggacc gacactgcgc cagaaagccg tgggaatgcc gggcggctcg
     6361 tgccggacat attccaagcg catatttggg aaggcgtaag gcggctctac cgcgccttcg
     6421 atcgtgtcga agtcgagatc atccttgaag gcgggtggcg cccaccgcgc aatgacggag
     6481 gagccgcaga tgcgatgatg ccatgccacc ggcaggcctt gcgcgtcgag ccccgccgtg
     6541 agccgatcaa agaaatacgg ccggtacata tcgtgctgga tgtcctcttc gcgggtccag
     6601 atcactttca cgggcccttc gacctgcttc gcaattttga ccgcctgggc gataaagtcg
     6661 acttcgaggc ggcgaccgaa tccgccgccg agcaagtggt tatgcacggt gacccgctcg
     6721 ggcggcagtc ccgtgatcga cgccgccgta gattgcgccc gcgtcatcac ctgggtcccg
     6781 acccagattt cacagccgtc tttgcggaca tggaccgtgc agttcatcgg ctccatggtg
     6841 gtatgagcga gaaacggaag ctgatagatg gcttcaagct tggtcttcgc gtccgccaga
     6901 gcctcttcaa cgttgccggt gttgcgcgca accgcacccc gcttctggga cgccgcctcc
     6961 agctggccca caatgtcggc cgtggtgagc gtcgcgtggg agccttcgtc ccactgaatg
     7021 tcgagcgcag cgaggccctt tttggccgcg cccatgtgtt gggcgaccac ggcgaccgca
     7081 tcttccagat gaacgacctg gctcacgccg ttcacagcca tcgccctgcc gtcgtcgacg
     7141 ctcttcagct tgccgccgaa caccgggcag gccgcgaccg tcgcgatctt catgcccggc
     7201 accttggcgt cgatgccgaa cagtgcggtt ccgtcgactt tggcgggcgt gtcgagacgt
     7261 ttgaccggct tgccgatcag cctgaaattc ttccgttcct tcagcggaat ttcggagggc
     7321 accggaagtg ccgccgcctt ctcggcaagc gcgccatagg aaaggcgacg acccgattct
     7381 ccatggatca cctcgccctt ctccgcccgg catgatctag gatcaacctt ccattcctca
     7441 gccgccgccg cgacgagcat catgcgcgcg gcggcaccgg ccttgcgcag aggctcccag
     7501 gacgcccgga ccgccgtcga gccgccagtc gcttgaaatc caagtagggg attagcgtag
     7561 aacttgtcgt tgggaggcgc atgctcgtat tgcacctgat cgagatcgac ctcgagctct
     7621 tcggcaatca gcatggaaat cgaggtgtag gtgccctgac ccatctcgat gtagggcatg
     7681 gtgaggtgca ccctgccgtc gcgatcaatg cggatgaagg cgccgatcac ctcgcgcgag
     7741 atggcaggcg cggcgccgcg ggccggaagg gacagcttca tgctgagcaa tagaccgccg
     7801 cctgcggcga gacttgcagc gagaaacgtg cggcgggaaa caccgtcgct ctcggctttg
     7861 aacctgttgg ctgggacacg cggcatcggt cagccctttt cgatgtccga tgagagggac
     7921 ttggccgcaa ccttgattgc ttcccgaatg cgcagatagg tgccgcagcg gcagatgttg
     7981 ccggacattg cgttgttgat gtcctcgtcg ttcggattgg cattggcaga cagcaaagcc
     8041 gtcgccgaca tgatttgtcc tggttggcaa tagccgcatt gcacgacctc gagatcgagc
     8101 catgcgctct gcacgctgcg accaaccggg gactcgccaa tcgcctcgat cgtcgtgatc
     8161 ggtgcctcgc ccacgctttc gatcggcatc acacaggagc ggaccgggtt tccgtccagg
     8221 tggacggtgc aagcaccgca caaagctctg ccgcacccaa atttcgtgcc cgtcattccg
     8281 agcacgtcgc ggagcaccca taacagcggc gtgtcgctat cgacatcgac gctgtggctg
     8341 acgccattga ctttgatcgt gaacgccatc gccgacatgt ttcattgcac tactaggcgc
     8401 agtctaagag agatggtagc ctttatccac gcttattgtc gtcgagtccg agaacgagag
     8461 atgaagatcg tttctggcac tctcgcagcg gccttttttg ccgtgctcac aattgctgtg
     8521 gtctgtgccg agcaaccaag cgctgagcga caggcgctga agccgcccga ggatttcgcc
     8581 caaatcgccg accagaacga acgttccttg gctctcttcg gtgaggccgc caaggtcatc
     8641 acgcatccgc gctgtgtgaa ctgtcatccc gccggcgatc ggccatcgca gggagaggat
     8701 agccacccgc atttgcctct cgtggtgcgg gggcctgcag ggtttggcgc cgtcgcgatg
     8761 cagtgcgtga actgtcatgg agcgaaaaac tacgacgccg cgcgcatacc cggacatccg
     8821 aagtggcact tggcgccgat cgagatggct tggcaaggca aaacgcttgg cgagatctgc
     8881 gaacagatca aggatccgaa gcgaaacggc ggaatgacgc tcgaagacct cgtgcatcac
     8941 atggccgagg attcgctcgt cggctgggga tggaatcccg gcgcgggtcg cgagccggcg
     9001 ccaggcacgc agaaggagtt tggcgctctg gtggaagcat gggtggcgag tggggcggct
     9061 tgtcccctgc catgaggctc tgcgcgtgga caaacgacga ttaggtcatt ccgtcagcga
     9121 tgccccggtt gtttgcaggg acagatgaac gtcagagagg ccaacccaaa gtatgggtca
     9181 ggatttgccc gtcgcatcgt ccacaacgac agcggtcgac ttttttgacg caaggattcg
     9241 cgcctcgtcc tttgtctcta ccgcagtcgc agtgactccc tcaggcgaga ccgtcaccac
     9301 caaataaggc attcccggtt tcggggggtg atagattccg gtcttggttt tcttgccttt
     9361 gggcatagct cgacccaatg ccacgtaagg tgctcggcca acgcgccgag acagcgactt
     9421 gactgcgttc tgttcatgcg cacgccaaac ctggctacga gagcttgctg tcataatcaa
     9481 ccactcagga aaagccccga cagctaagcc ggggctgagg cggtccagtg cggcagggtg
     9541 gatcctgtcg cgctcaaccg cttttgcgtc tcttgctggg cttcctcttg ctgcggccgc
     9601 tggctcttcc gcgaagactg tcagacggat gcttgacggt caccccgcgt gtcggctttg
     9661 aaatcggcac catatcgggt acctcaccga taactaggaa tgggatctcc tcctgatcga
     9721 tacgagccgt gctcgcgctc gtgttccagc taatcgtaat cgaatcgcgc gtaacggtat
     9781 cattcagttt gacacgcggg ttgctggaga gcgctagaat ggcgaattga cgtgccgcaa
     9841 ccatcgttac gggtcgccgg aggaatcttt cgcggggacc aatttcctgt tcctttctca
     9901 attcgaacgc gggattgctg cgcgcggaaa gctgaaacgg gttgaagctg atcacgacga
     9961 tgccccgagc ggtaccgccg ttcggaacga atatcattcc gctgaggatc ctgagtgcca
    10021 tgcctgccgc ctccgttgcc tgtcatggga tcggtactct aaccgcgacg gtatagcacg
    10081 atgcgatcag ctaagctctc gcctccggat tgggtcttct ctctcaccgg tgatccgctt
    10141 cttgcatttg aactattttt gcagcgattt agcttccttg gcgaggagcg cgcgcttgcg
    10201 ttcgacgccg ccgcgatagc ccgacagcga tccgtcgctc gccaccacgc ggtggcaagg
    10261 gatcgcaacg gcgatgcggt tcgatgcgca agcgcgggcg accgcgcgca ccgcgcgcgg
    10321 cgcaccgatc gccttggcaa tctcggcata gcttgccgtt gcgcccggcg gaatgcggcg
    10381 aagcgcctcc caaacgcgat gctgaaacgc ggtgccgcga atgtccaagg ggagattgag
    10441 gtcctcgcgc gggttctcga cgtaggcgat tacctcggcg gtgacccggt ggaagctcgg
    10501 atccccctcg atgagccggg cgcgcgggaa ctgacgctgc agatcgccgc ggagggatac
    10561 ggggtcgctt ccaaacagga tcgcgcacac gcccttcgcg ctcgcggcga cgagaactaa
    10621 gccgagcgag catttgccga ttgcgaattt gatgtccgca tcgggtgcgc ccttgcggaa
    10681 ttggcctggt gtcatgccaa gagcctccga agacgtcgca tagaaccggc tgctcgaatt
    10741 gaagcccgcg ccgtagatgg cttcggtgat cgtcgcgctg cggcctagct ccccgcgcac
    10801 gcgcttgcgg cgttgagctg cggcatagcc cctcggcgtg atgccaagca tcgacttgaa
    10861 gatgcggtgg aaatgatagg ggctcaagcc caccgcggcg gcgaggtcgc caagcctcgg
    10921 cgcttcttca gcctcttcga tcatccggca ggcgactctg accttggccg cgtgctcttg
    10981 aatgagggac ggctcgttcg gcttgcagcg cttgcagggg cgaaaccccg cccgctcggc
    11041 ctcggccgcg gttcggtgaa aacggacatg cgcgcggttg gggcgtcgcg cggcgcaaga
    11101 ggggcggcaa tagatgccgg tggtcgcgac ggaatagaag aagcggccat cgaaggcggc
    11161 atcgcgtgcg agaaccgcaa gccagcgcgc ctcttcgaga gccggcgacc gggcgccgtt
    11221 tgccacggcg gggtgggcca ggatggcggc cattctcttg ctccctttca tcagtgcgtt
    11281 cctgcgacaa ggcttaggcg gatgtggccg ccggcgcacc ccgatccttg ctctcaaatc
    11341 tatggcgaaa cggcccgtgc gatcatatca ggagaaggca agagcgggcg gcgctttgcg
    11401 gcgcttgtct ttttgtcacg gccaagacat caaccccatc aaaccgtcgc aaaaacgtga
    11461 taatccacag gcacacagcg tcgggcaaag tgcattgaac gcttccttgc aaagggtggg
    11521 tgaaagacct tgagcgaccg agcggagcct ggcttcatta actcgggcct ccttgggcgg
    11581 atcggacagg ccgtgctgcg tccgttccgc gagatcgcgg cacggctgct cgcaacgcta
    11641 tggctgatcg tggccgtcgg cgtgatcatg gtctctctcg tgttgatcgc aggcctctcc
    11701 aaaaaattcg ccctgatcgg ctttgtcagt ttcgtggcct cggcaatgct gttgccacgg
    11761 cacgagaggg atcaggcgct acccagcgcg cttgggccgg cctcgccgtc agtgcctgcg
    11821 gtcgcggcca tgcgcgtgct cgccgaggcg ttgccggacc ccgtcatcct gctcagtccg
    11881 ggagggcatg tcctcttctg caatgctcag gcgcgctctc tcttcgcgtc gctgcgcgag
    11941 ggaagccata tctcctcggt gatcagaacg ccggaattcc tcgacgccgt cgcggccgcg
    12001 cccgggctcg gccgccccgt gaccgtcacc tatgccgagc gcgttccggt cggccgccgc
    12061 atggcggcga cggttgcgcc cttggccgga agaggcgagg aggccggcct cgccaatatc
    12121 ctcgtgctgc tccgggactt gaccgaaatc gagcgcatca accagatgcg cgccgatttc
    12181 atcgccaatg cgagccatga actccggacc ccgctcgctt cgttgcgtgg cttcatcgaa
    12241 acgctgcaag gacgcgccaa gggcgacccg gccgcgcgcg accgcttcct tccgatcatg
    12301 gccgagcagg cctcgcgcat gacgcgcctc atcgatgcgc tgttgtcgtt gagccgggtc
    12361 gagatgaacg cgcatgtgcc gccgtccgat ctcgtcgatc tcaacgatgt gctcggtcac
    12421 gccaaagaca cgctcgaacc gttggcgcgc gacatgggct tcacgttcga ggtcggacga
    12481 tttccccgtc ccgcaatcgt gcgcggcgac cgcgacgaac tgatgcaggt ggttcagaac
    12541 ctcgcgcaca acgccttcaa atatggggtc aagggcggca cggtgcggat cgaggccaag
    12601 catattcccg cgctcggccg ccagaccgga cgctacacga tcgccgtcat cgacaatggg
    12661 cctgggattg cgccggagca tctgccccgc ctgacggagc gcttctaccg ggtggatgtt
    12721 gccgcaagcc gggagaaagg cggaaccgga cttgggcttg ccatcgtcaa gcacatcctc
    12781 aatcgccacc ggggcgagct tgcgatcgcg tccgtgccgg gcaaaggctc gaccttcacg
    12841 gtgatgctga acgaggtgcg cgcacatagc cccgaacggg gcgccgccgc cggctgaaac
    12901 gggcgaccgt ccacatgcta ctggaacagt agccgctcac atgaccgatt tgacggatat
    12961 tgcggacggg gtcgccgagg cagcgaatgt ccgctttgtg ccatgtgtgg acggctcccg
    13021 gttggcaagt caaaacgtca cgtcgcgtcg ctggtcggtg cagccacgtg ttctgcctgt
    13081 tagcgcggtt cgcatgaccg ctggccataa tgccctccgc ggatcgggtc ccggtcatta
    13141 gcccgcattc gataatgcac tggcacatgt gggttgtccc gatcgcccga tcggcgtctt
    13201 ggtctcgaca aaccacatat tggccctggt gaccgcttca caggtggctt cggcgtcggt
    13261 ggcatcattc ttctgccgct tgacgtaggg ggggcgctgc cgccggctga gtaggcgcgc
    13321 tagcgcgccc gcgggccacg ccacggggca ccaagggccg ctgacgcacc gatgtgttgt
    13381 ttaacggccg gacaagagca ctgagcctcc cccacttatc cacacctgtg ctacagaaat
    13441 tctcgtataa tcaaatgtat agactgtcat aaagccgaat catcggcgtc ataaaacaat
    13501 gatccacgat gactagcgtg cccggcgagc tggcggcgga tcgatcgccg gctgaggctt
    13561 catccaagca agggagagca aattgaacag gattggtatc gtggcgtcga cgctcgccgt
    13621 cgccgctctt tgcacgaccg gcccggcgct ggcacgtgat caactcaagg tcgtcggctc
    13681 ctcgacggtc ttcccctatt cccaggcggt cgccgaggat ttcggcaaga agaccggcaa
    13741 cgaggccccg gtcgtcgagt cgacaggcac cggcgggggc atgaagatct tctgccaggg
    13801 cgtcggcgag ggcaacccgg acgttaccgg cgcatcccgc gccatgaaga agtcggaatg
    13861 ggagctctgc gcgaagaacg gcgtcaacga catcaccgag atccagatcg ggtatgacgg
    13921 cttgtcgatc gcccagtcca agaagggcaa tcccatcgat ctcagcaagc agcagttgtt
    13981 cctcgcgctc gcttccgaga ttcccgacgg cgacaagctc gtcgccaatc ccaataagaa
    14041 atggagcgat gtcgataagt cgcttcccgc tgtggcgatc accgtctatg ggcctccgcc
    14101 gacctccggc acgcgcgacg cattcgtcga gcttgccatg catgagggct gcaagtcgct
    14161 cgattacttc aagaagaaga aaggcgagct cgacaaggac gggttcgaca agcttatcaa
    14221 ggagaaatgc acgcccatgc gccaggacgg cccgttcatc gaggcgggcg agaacgacaa
    14281 tctgatcgtg cagcggatcg aggccgaccc caacgcgctt ggcatcttcg gctattcgtt
    14341 cctgtacgag aaccaggaca agctccaagg cgtaaagatc ggcggcgtgc agccgacctt
    14401 cgacaccatc gccgacggca gctacggtct gtcccgaccg ctcttcatct acatcaagaa
    14461 tgcccatcgc aaggtgatcc ccggcatgaa cgagttcatc gccgagtaca cctccagcga
    14521 ggcaatgggt cccgacggct atctgcatga gcgcggcttg gtcgtgctct cggatgacat
    14581 gctgaaggag atgcaggacc gcgccaagag cgcggacaag atggctgcgc cgacatccta
    14641 ggcgagccgt cggaaaacag ggctgattgg ggcgtggccg tgcgcgattt ttcgcggcca
    14701 cgcccgcaat cttatgaggc aacgcgcgga ttttgggggc tgtggcttga gccacgaagg
    14761 cgggcgcaat gattggctac gttctccttg ggatcatcgc gcttagccta ttcagctacc
    14821 tgctcggccg gcgccgcgcc atggcctcgg tcggaggccg tcagcaagag ctccattccc
    14881 gcccaagcta ccacggggcc tttgtcgcgg cctgggtcgg cattccatcg atccttctcg
    14941 tcctcgtctg gctggtgctc cagggccctg tcatcgacag gatgctgctg tggagcctgc
    15001 cgccgggcgc gaccgacggc ctcactagcg ggcagatcga cctcatgctg agcgagatca
    15061 ggtcggtcgc ggcgggcaat atcttcagcg aaccgagcct ggtcatccag gaggcggcgg
    15121 aacgctatca gcgctggaag gcgatcgccc gttacgcgat ggtcgtcgtc gctttgtcga
    15181 tcgccctgtt cggcttgttc gtcgcccggg cccgcatcaa ccccgaattt cgggcccgcc
    15241 gtgccaccga gcgggtgatg agcgggctga tgatggcctg ctcgttgatc gccattctca
    15301 ccacgctcgg cattgttctg tcgcttctgt tcgaggcatt gcgctttttc gatcgtgtct
    15361 ccccgctcga gttctttttc gggctcaatt gggaacccca gattccgatt cgcgagggcc
    15421 aggtgacggc cggcggctcg ttcggcgcca ttcctgtctt caccgggacg cttctgatct
    15481 cggcgattgc cttggccgtg gccatcccca tcggtctctt gtcggcgatc tatctgaccg
    15541 aatacgcgca cccgaagttc agagccgtcg tcaagccgat gctcgagctt ctcgccggca
    15601 ttccgacggt ggtcttcggc ttcttcgcgg tgcttgtggt ggcaccgtcc atgcgcgatt
    15661 tcggtgcgat gatcggcatt ccgatcgcgc cgaacagcgc gctcgccgcg ggcgccgtca
    15721 tgggcatcat gatcatcccc ttcgtgtcgt ccctgtcgga tgacgcgatc gccgccgtgc
    15781 cgcgctccat gcgcgatggg tcctacgcca tcggcgcgac caaaggcgag accatcacca
    15841 aggtgctcct gcccgcggcc ttgccgggga tcatgggcgg aatcctgctc gcggcgagcc
    15901 gcgccattgg cgagactatg atcgtcgtca tggccgccgg catcatcgcc tcgctgacgc
    15961 tcaatccgct cgaacccgtg accaccgtga ccgtgcagat cgtgacgctc ctgatcgggg
    16021 atgtcgagtt cgacaatccg aagacgctcg ccgcgttcgc gctcggcctt gtcctcttcg
    16081 tcgtgacgct cgctctcaac gtgctcgccc ttcacatcgt gcggaaatat cgagagcaat
    16141 atgagtgacg cacaagccat agagccccgc ccgcttgcct gcgactcggc ctccgataaa
    16201 gcgcggaggc gcgtgaagcg acgctatgcg gccgaccgaa gattgcaggc ctatggcatc
    16261 atcgccatcg cgctcgccgt cggcctcctc ggcgtgctca tcacctcgct catcgtgaac
    16321 ggcctgccgg ctttcgttca gaccaaggcc gaccttgccg tctttatcga tgccgccaag
    16381 gtcgatgccg agaaccccgc cggcggcaat taccgtgctc tcgtgcgtga ggcgcttgag
    16441 cccttcgtcc ccgccgatgc caccgacaag cagaagaccg atgctgcgaa aattctcaca
    16501 tccgacgcgc cgtatatcgt gcgcgactat atccttaagc atcccgaggc gatcggcaag
    16561 acggtcgcgc tgcccatccc cttgtccgat ccgttcgatc agctgaacaa gggcgtgatc
    16621 ccgatggacg tcgacgcgct ttcttggagc caggtccgct atttcgagcg cctgcggaat
    16681 cgcggtgtca tcgtcgagga cgacggacgc accggcctta agctggacgt ttatgtcgat
    16741 ccggccaaga tcgagcttgg ccccccgccg tccggcgact tcgcgacgct cgcccgggat
    16801 ggtcttcagg cctatttgcc cgacgtgcat gacacggcgt tgttcgccat gctggccgac
    16861 gatgcgccgg aggcgatctc cgcgcgcgtg gccgccgatc cggccctcat tggcaaaacg
    16921 atacaggtga ttttgccggt gtcgccggcc gttaccgcgc tggccgaggg caagagcccg
    16981 aaaattctca atccgcgctt caaggccgat cagatcgaga cgttcgaagc ccttaaggcg
    17041 aaggggctcg tgcatgcccc gttcaactgg gagctgttct tcaacgccga cagccgcttc
    17101 ccggagcgcg ccggcctcgc cggcgccatc acgggcacct tcctaatgct tctcgtctgc
    17161 ttcgggttga gcttccccat cggcatcgcc gcggccgttt atctcgaaga attcgcgccc
    17221 aagaaccggc tcaccgatct catcgaggtc aacatcaaca atctcgccgc cgtgccctcc
    17281 gtggtgttcg gtctgctcgg gcttgccgta ttcctcggct tcttcggcct gccgcggtcg
    17341 acgccgctcg tcggcggcat ggtgctttcg ctcgtgaccc tgccgaccat catcattgcg
    17401 acccgtgccg cgctcaaagc cgtcccgccc tcgatccgcg aagcagcact cggcgtcggc
    17461 gcttccaagc accaggtcgt cctgcatcat gtcctgccgc tcgccatgcc cgggattctc
    17521 accggcacca tcatcggcat ggcccacgca ctcggcgaga ccgcgccgct cctcctgatc
    17581 ggcatgaacg cattcattcc aagcgtcgag agcatgggcg ttctcgagcc cgcgaccacg
    17641 ctgccgacgc aaatcttcag ctgggccgac agtccggagc gcggcttcgt ctcgcgaacg
    17701 tctgccgcca ttctcgtgct attggcattt ctcgtcgtca tgaacctgct cgccatcgtg
    17761 cttcggcaga aattcgagcg taaatggtag aaggggattc ggttatggag gctaaagtcg
    17821 cggcgcggcc agtggtgcgg gtgcagatgc cagtgccgcc tgaggcgacg gagggcatgc
    17881 tgccggccgc cgagcgcgag ctcaagatga ccgcccgcga cgtcaatgtg tggtacggcg
    17941 aaaagcaggc gctgttcgac gtctcgctcg atatcttcga tcgctcggtg acggcgctga
    18001 tcggtccttc cggttgcggc aagtcgacat tcctccgcgc cctcgatcgc atgaacgacg
    18061 tgatcgaggg atgccggatg accggcgaaa tcacactgga cggcgaggac atctattcgc
    18121 ccgaggtcga tccggtgcaa ctccgcgccc gcgtcggcat ggtgttccag aagcccaatc
    18181 cgtttccgaa atcgatcttc gagaacgtgg cctacgggcc tcggattcac gggctggccg
    18241 gctccaaggc ggaactcgaa gagatcgtga tcaagagcct gacccgcgcg ggactgttcg
    18301 aggaggtcaa ggaccgcctg aacgagtccg gcaccggcct ttctggcggt cagcagcagc
    18361 ggctctgcat cgcccgcgcc atctcggtga acccggaagt cctcctcatg gacgaacctt
    18421 gctcggcgct cgacccgatc gccacggccc gcatcgaggc cttgatggag gagctcaagg
    18481 agtcgctctg catcgtcatc gtcacccact cgatgcagca agcggcccgt gtctcgcagc
    18541 gcaccgcctt ctttcatctc ggcaagctgg tcgaggtgga cgatacggtc agaatgttta
    18601 ccaatccggc cgatacgcgc acgcaggatt acgtcaccgg ccggttcggc taaccggccc
    18661 gcgggagttt tgaaatgatg gacaagcaac acgaacacat cgtcaggtcc tacgaggagg
    18721 agcttgaact cctcaacaac aagatcgcca agatgggcgg tctcgccgaa caggtgctcg
    18781 gccagtcgat cgacgcgctg gagcgccgcg atcccgagct tgccgcctcc accatcaacg
    18841 aagacgaggc gatcgatgcg ctggagaggg agatcgaaga gcaggcggtg gtaatgatcg
    18901 cgcggcggca gcccatggcc tacgatctgc gccagatcat ggcggcgctt cgcatcgccg
    18961 gcgacctgga gcgcatcggc gaccttggca agaacatcgc caagcgcgct gttgccgtgg
    19021 tgagcgagca gcagccgaag cagctcatgc tcggcctcaa gcatatgggc gaactcgcgc
    19081 tggcgcagct caaagaggtg ctcgacgcct tcatcgagcg cgacgccgac cgggcgctca
    19141 aggtctggta caaggacgaa gagatcgacg ccatgtacaa ctcgctgttc cgcgagttgc
    19201 tcacctacat gatggaggat ccgcgcaata tcgggctctg cacgcacctc ctcttcggcg
    19261 ccaagaatat cgagcgcatc ggcgatcacg ccacgaatat cgccgagacc gtgtactacc
    19321 tggtgcacgg cgccgccatc accgatcagc gcccgaaggg cgatacgacc agctcgacgc
    19381 cggtgccgct gaagcgatcg gcgggatagg cgagggcacg cgatgagcgc caccagcccc
    19441 ggagccaagg tcctcgtcgt cgaggacgaa gagccgctcg cccttctcct gcgctacaac
    19501 ctcgaagccg aaggctatgc cgtcgatgtc gtgcatcgcg gcgacgaggc ggaaatggcc
    19561 attgccgaga gcacgccgga c
//
LOCUS       DASUQP010000218         3478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1197894, whole genome shotgun sequence.
ACCESSION   DASUQP010000218 DASUQP010000000
VERSION     DASUQP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197894"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFY74_09905"
     CDS             complement(<1..428)
                     /locus_tag="VFY74_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX5932047.1"
                     /translation="MVGTLTFDQLKEAVTGTGEIDTVVACFPDMQGRLIGKRFQAGFF
                     LDAAYEETHGCDYLLADDIDMEPVPGYAAASWERGYGDFVMKPDLSTLRRIPWLPATA
                     LVLCDVLDHHHHEPLTHSPRAILRQQIARLADNNMSSRMAS"
     gene            complement(520..2001)
                     /locus_tag="VFY74_09910"
     CDS             complement(520..2001)
                     /locus_tag="VFY74_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5932048.1"
                     /translation="MADKKSPEGIAYRKAEAGYFEKRGFRRYAGVWSLWALGVGAVIS
                     GHYSGWNLGLATGGWGGMLVAGILIMIMYIGLVFCIAEMAPALPHTGAAYSFARSAMG
                     PWGGFITGLCENVEYVLTPAVIVFFIGSYMGGIFETPANFQPVWWVLMYAAFLALNIV
                     GVELSFRVTLAVTLAALVVLVIFWISAIPNVDFNRWALNIAPDGSELPEGNGPYFPFG
                     INGILATLPFAVWLFLAIEQLPLAAEESVDPKRDMPPGILLGMLTLIISAGMIVWLNP
                     SVIGVGSFALGKSGEPLLDGFRAIYGGAAAKVLAFVAVVGLIASFHTIIYAKGRQIYS
                     LSRAGYFPTGLSVTHSGYKTPHIAMIVGALVALAVMFAVWFGVGSERGAAIIGGTLLN
                     MAVFGAMFSYILQAVSFIMLRRNLPNIERPYRSPLGIPGAVVTIIIGLVTVYYQLADP
                     VYRNGVIAVACWFVIGILYFALFARHRMILSPEEEFAMEHRKD"
     gene            complement(2044..2892)
                     /locus_tag="VFY74_09915"
     CDS             complement(2044..2892)
                     /locus_tag="VFY74_09915"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5932049.1"
                     /translation="MPIPNQLAEPSRGGVVGGTDQDKARPRLRERSLIVGENPGGSGP
                     FVFVCDHASNRIPQEFKSFGFAEEALATHIAWDPGALGVARHLSARFDGPLFWPDVSR
                     LVLDCNRAPNAKSLIVTESEGRPVPANLALTAAERSWRLDSIHSPYHAAIDSCLGRRL
                     AGRQPTALIAVHSYTPIYFGKARPWQVGIVFAEDRRMADLLIRGLKADPALTVGINEP
                     YSPADQVYYTVERHAGARGMPAAMIEIRNDEIGDEAGQRKWADRLADILSAAERTLAL
                     ASRTVG"
     gene            3283..>3478
                     /locus_tag="VFY74_09920"
     CDS             3283..>3478
                     /locus_tag="VFY74_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX5932050.1"
                     /translation="MAATLVAGLAFPAQADDKKITLSATTVLTTDYMFRSISNTEERP
                     AAQAEFDLYYGIFYAGIWGSN"
BASE COUNT          719 a         1070 c         1080 g          609 t
ORIGIN      
        1 gaagccatgc gtgacgacat gttgttgtcg gcaagccgcg caatttgctg gcgcaggatg
       61 gcgcgcggac tatgcgtgag cggctcgtga tggtgatggt cgagcacgtc gcacagcacg
      121 agcgccgttg ccgggagcca agggatgcgc cgtagcgtcg agaggtccgg cttcatgacg
      181 aagtcgccgt agcctctctc ccagctcgct gccgcgtagc cgggcactgg ctccatgtcg
      241 atgtcgtccg cgagcaggta gtcgcagcca tgggtttcct cgtaagccgc gtcgaggaaa
      301 aacccggcct gaaaccgctt gccgatgagc ctcccctgca tgtcgggaaa gcaggcgaca
      361 accgtgtcga tctcgccggt gcccgtcacg gcttctttga gctgatcgaa ggtcagagtt
      421 ccgaccatac gacgatactc ccgttccggg atggagaggc ccgcgccaac tgcgcgggcc
      481 tcttaccgtc atcccaactg aggctggcga tgatgcgtgt cagtccttgc gatgctccat
      541 cgcaaactcc tcttccggcg agagaatcat gcgatgccgg gcaaagagtg cgaagtagag
      601 gatgccgatc acaaaccagc aggcgaccgc aatcacgccg ttccgataga ccggatcggc
      661 gagctgatag tagaccgtca ccaacccgat gatgatcgtg accacggcgc cgggaatgcc
      721 gagcggactg cgatagggcc gctcgatatt cggcaggttg cgccggagca tgatgaacga
      781 gaccgcttgc aggatgtagg agaacatggc gccgaaaacg gccatgttca ggagcgtgcc
      841 gccgataatg gcggcgccgc gctccgagcc caccccaaac cagaccgcga acatcaccgc
      901 caacgccacg agcgcgccga cgatcatcgc gatatgcggc gtcttgtaac cgctatgcgt
      961 gaccgacaac ccggttggaa aatatccggc gcgcgacagc gaatagattt gccgcccctt
     1021 ggcgtagatg atcgtatgga agctcgcgat cagaccgacg actgcgacga aagcgaggac
     1081 tttcgcggcg gcgccgccgt agatggcgcg gaagccatcg agcagcggtt cgcccgactt
     1141 gccgagcgca aaggagccga cgccgatgac cgacgggttg agccatacga tcatcccggc
     1201 cgatataatc agcgtgagca tgccgaggag gattccgggc ggcatatcgc gcttcggatc
     1261 gaccgattcc tcagccgcaa gcggcaattg ctcgatcgcg aggaacagcc acacggcaaa
     1321 cggcaacgtg gcgaggatcc cattgatccc gaatggaaaa tatgggccgt tgccctccgg
     1381 cagctccgag ccgtccggcg cgatgttcag tgcccagcgg ttgaaatcca cgtttggaat
     1441 ggcactgatc cagaagatga caagcaccac aagcgcggcg agcgtgaccg ccaaggtcac
     1501 gcggaacgaa agctcgacgc caacgatgtt gagggcgaga aacgcggcat acatcaacac
     1561 ccaccatacg ggttggaagt tggccggcgt ctcgaagatc cctcccatgt aggagccaat
     1621 gaagaagacg atgacagcgg gcgtgagaac gtattcgaca ttctcgcaga gtcccgtaat
     1681 gaagccaccc caaggtccca tcgccgatcg cgcgaaggaa taggccgcgc ccgtatgcgg
     1741 gagcgccggc gccatctcgg cgatgcagaa gacaaggccg atatacatga tcatgatgag
     1801 gatgccggcc accagcatcc cgccccaacc tcccgtggcg agtcccagat tccagcctga
     1861 atagtggccc gatatcaccg cgcccacacc gagcgcccac agcgaccaaa cgccggcata
     1921 acgccggaac ccgcgcttct cgaaatagcc ggcctccgcc ttgcggtagg cgatgccttc
     1981 cggacttttt ttgtctgcca ttgcttcctc cctatgtgcc ccccatacgt gccctgcaag
     2041 atgtcatcca acggttcggc tggctaaggc cagagtccgc tccgccgcag agagaatatc
     2101 ggcgagccgg tccgcccatt tccgctgccc ggcttcatcg ccaatctcgt cgttgcgaat
     2161 ttcgatcatc gccgccggca tcccgcgcgc gccggcatgc cgctcgaccg tgtaatagac
     2221 ctggtcggcc ggcgaatagg gctcgttgat gccgacggtg agcgccgggt cggctttgag
     2281 cccgcgaatc agaagatcgg ccatgcggcg atcctcggcg aacacgattc ccacctgcca
     2341 cggtcgggcc ttgccgaaat agatcggggt ataggagtgg accgcgatga gcgcggtcgg
     2401 ctgacggccg gcaaggcggc gccccagaca tgaatcgatc gcggcatgat aggggctgtg
     2461 gatcgaatcg agccgccacg agcgctcggc tgcggtcagc gcgaggttcg ccggcaccgg
     2521 ccgtccctcg ctttcggtga cgatcaggct ctttgcattg ggggcgcgat tgcagtcgag
     2581 gacgaggcga gacacgtccg gccagaacag cggtccatcg aacctcgccg agagatgccg
     2641 cgccacaccg agcgcgccag ggtcccaggc aatgtgagtc gcgagcgcct cttcggcgaa
     2701 cccgaaagac ttgaactctt gagggatgcg gtttgaggcg tgatcgcaga cgaatacgaa
     2761 tggcccggaa ccgccgggat tttcccccac gatcaggctc cgctcccgga gccggggcct
     2821 cgccttgtcc tggtccgttc ctcccaccac cccgccacgt gaaggttctg cgagctggtt
     2881 tggaatcggc atgtcgcttg ctcgatgcgg catcaaagag gcgccccctg agcgcggccg
     2941 ggcgctaaga gggcttttct aatcatagtc ttcgataact caatcccgca aatctctatt
     3001 tgcccagttt tcgtgcgagt tgagcccaaa gcgggaccaa acctcccttt gactatggca
     3061 aatcggccac agcgtcggcc tctttttgcg agggcgcggg tttttcattg cgtcgggggt
     3121 agggcggcat attctgtgcg gtaacgggga acattgcatc gcaaagccgt gagaagcgca
     3181 cgcgggcaat gccgaacggg cagtgagatg ggggactgaa aaatggctcg gcgcaacagg
     3241 gaagtgggcg tcattaagtc atgcgttttg aagtcgtgcg ttttggctgc gactctcgtc
     3301 gcggggttgg cgtttccggc tcaagccgac gataagaaaa taacgctttc ggcgacgacg
     3361 gtgctcacga cggactatat gttccggagc atctccaaca cggaagagcg tcccgccgcc
     3421 caggcggaat tcgacctcta ttacggcatt ttctacgccg gcatctgggg atccaata
//
LOCUS       DASUQP010000219         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_107737,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000219 DASUQP010000000
VERSION     DASUQP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107737"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFY74_09925"
     CDS             <1..215
                     /locus_tag="VFY74_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932051.1"
                     /translation="AGAPPSAIEVPMTDEPGKEASEAKQAVPGKSDAKGSAPKKFAPR
                     TAKSQNKFAPFNTLSELLAPPAQKRR"
     gene            complement(254..2203)
                     /locus_tag="VFY74_09930"
     CDS             complement(254..2203)
                     /locus_tag="VFY74_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5932052.1"
                     /translation="MASLGSLNKYWYWALLLAVGAGVAAAALVAPGKISRLELEREAS
                     VAAARLKTKMLQEPDALFHALGRPSSTPQFKDILDRTGYGHRVLRYELYGRDGQLAFT
                     SGLAGLKLEQELASLLTSPASEEAKVTLYESTAESSPTNFAAVALPLALNGEPRGTLV
                     VYLDQSDQAKVLSGYFGWVTAITLSLLGAGIAVPAAFAWTRGRERRQAEAQVRYLEEH
                     DALTGLPNRKSFSDRLADAIGRMHRDRANIAVLCLDIDNFKEINDAADHSGGDQVLRD
                     IGARIQTTLREGDLVARTGGDEFAIGLIHIANLGEVMPFMNRLVEVLQKPFQVGGKEM
                     RVTVSAGAALVPADGDSEAMVLRHAAIALARAKSDGGQRMCFFEESMNKALQRRRMVE
                     HELRLALGRGEFEVVYQPQFDLATERQCGSEALIRWHHPVHGKIAPAHFIAVAEDTGL
                     IVPLGEWVLRRACDDAAKWPENLFVSVNLSPAQFRHGDVGALVGQVLKETGLPPGRLE
                     LEITENLLINDTEEVLGKLSRLRQLGVNIAMDDFGTGYSSLSYLARFPFSKIKIDRQF
                     IRSMTRDPAMRAIVKTIIALGKSLDVIIIAEGVETEEQAAMLREFSCPQVQGFLYGYP
                     GPADANLMDLSGNVTPITAARGSAA"
     gene            complement(2356..3354)
                     /gene="moaA"
                     /locus_tag="VFY74_09935"
     CDS             complement(2356..3354)
                     /gene="moaA"
                     /locus_tag="VFY74_09935"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5932053.1"
                     /translation="MTGSPLIDPFGRRITYLRVSVTDRCDFRCVYCMAEAMTFLPREE
                     LLTLEEIDRLASAFVAKGVRKLRLTGGEPLVRRNIMWLIRSLSRHLGNGLDELTLTTN
                     GSQLALHAEALAASGVARINASLDSLDAAKFRAITRRGDLAQVMGGIDAAARAGLKIK
                     INMVALRDVNEDEIEEILRWAHGRGFDLTLIETMPMGEIGADRTAQYLPLSLVRARLA
                     QHFTLDDDPYRSGGPARYVKVRETGGRLGFITPMTHNFCESCNRVRVTCTGTLYMCLG
                     QEDAADLRTPLRASSDDALLHAAIDAAIARKPKGHDFVIGREHRPSVARPMSVTGG"
     gene            3649..3843
                     /locus_tag="VFY74_09940"
     CDS             3649..3843
                     /locus_tag="VFY74_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX5932054.1"
                     /translation="MDEQRFNMSMRKYLKEVGVTSQQAIEEVVREEHLGGKGKLKMKM
                     VLTAEGTRLKHVIDGTIDLG"
     gene            3867..>4073
                     /locus_tag="VFY74_09945"
     CDS             3867..>4073
                     /locus_tag="VFY74_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HEX5932055.1"
                     /translation="MTEPWPTELKLDKGRRVLTVTYDDGVSFALPAEFLRVLSPSAEV
                     QGHSPEQRVTVAGKKSVGIARLEPV"
BASE COUNT          752 a         1253 c         1361 g          707 t
ORIGIN      
        1 ctgccggagc gccgccgtca gcgatcgagg ttccaatgac ggatgagccg ggcaaggagg
       61 cgagcgaggc gaagcaagcg gtccccggca aatcggacgc taagggttcg gcgcccaaga
      121 agttcgcgcc tcgcacggca aagagccaaa acaaattcgc gcctttcaat acgttgagcg
      181 agctactcgc accgccggcg cagaaacgga gatagaagca cgtttgcgct aaacccgtta
      241 ccgatcggcg cgcctaggcg gcgctgccgc gcgccgcggt gatcggcgtc acattgccgc
      301 tcagatccat gagattggca tcggccggtc cgggataacc gtagaggaag ccctgcactt
      361 gcggacaact gaactcgcgc agcatcgccg cttgctcctc ggtctccacc ccctcggcga
      421 tgatgatcac gtccagcgac ttgccgagcg cgatgatggt cttgacgatc gcgcgcatcg
      481 ccggatcgcg cgtcatgctg cggatgaact ggcggtcgat cttgatcttg gaaaagggga
      541 agcgcgcgag atagctcaag ctggaatagc cagtgccgaa atcgtccatg gcgatgttga
      601 cgccaagctg gcgcaagcgg ctgagcttgc cgagaacttc ctcggtgtcg ttgatcagga
      661 gattctcggt gatttcgagc tcgagccgtc ccggcggcag gccggtttcc ttgagcacct
      721 ggccgaccag ggcgcccacg tcaccgtgcc ggaactgcgc cggcgagaga ttgaccgaga
      781 cgaaaaggtt ttccggccat ttggccgcat cgtcgcaggc gcgccgcaaa acccactcgc
      841 caagggggac gataagcccc gtgtcctcgg cgacggcaat gaagtgagcc ggcgcgatct
      901 tgccatgtac cggatgatgc cagcggatca gagcttccga gccgcattgg cgctcggtgg
      961 cgagatcgaa ctggggctgg tagacgactt cgaactcgcc acgaccgagc gcgaggcgca
     1021 actcgtgctc gaccatgcgg cgccgctgca acgccttgtt catgctctcc tcgaagaagc
     1081 acatgcgctg gccgccgtcg ctctttgcgc gcgccaaggc gatggcggcg tggcgcagca
     1141 ccattgcctc cgaatcgcca tcggccggca ccagcgccgc accggcgctt acggtcacgc
     1201 gcatttcctt gccgccaacc tgaaacggct tttgcaggac ctcgaccagg cgattcataa
     1261 agggcatgac ctcgccgaga ttggcgatat ggatgagccc gatggcgaac tcgtcgccgc
     1321 ccgtccgcgc aacgaggtcg ccctcgcgca gtgtcgtctg aatgcgcgcg ccgatgtcgc
     1381 gcaatacctg gtctccgccg ctatgatcgg cggcgtcgtt gatctccttg aaattgtcga
     1441 tgtcgaggca aagaacggcg atgttggcgc ggtcccgatg catgcgtccg atggcgtcgg
     1501 cgagccggtc gctgaagctc ttgcgattgg ggaggccggt cagggcgtca tgttcctcga
     1561 ggtagcggac ttgcgcctcg gcttggcgcc gctcgcggcc gcgcgtccag gcgaaggcgg
     1621 ccggcacggc aatgccggca ccgagcaggg acagcgtgat ggcggtgacc caaccgaaat
     1681 agccggagag gactttcgcc tggtcgctct ggtcgagata aacgacgagc gtgccccgcg
     1741 gctcgccgtt cagcgcaagc gggagggcga cggcggcgaa attggtgggc gatgactcgg
     1801 cagtactctc atacagcgtg accttagcct cctcgctcgc ggggctcgtc agcagagagg
     1861 cgagttcttg ctcgagcttg agaccggcaa ggccgctggt gaaagccagt tggccatcgc
     1921 ggccgtacag ctcatagcgg agaacgcgat gaccgtaacc ggtcctgtcg agaatatcct
     1981 tgaattgggg cgtagacgat ggccgcccga gcgcgtggaa cagcgcatcg ggctcttgca
     2041 gcattttggt cttgaggcga gcagcggcga cggatgcttc gcgttcgagc tcgagccggc
     2101 tgatcttgcc cggcgcgacg agcgccgccg cggcgacgcc ggcgccgacc gccaacaaca
     2161 gcgcccagta ccaatatttg ttgagcgacc ccaaggacgc catggacgat cgccacgcta
     2221 cttccgaccg gcggccaatg tgggcggcga tgagttaaga accgggtaag cttgttgatt
     2281 aacaaaccat gcccacgatg ccgtagagtt gggggtatag ggaaaaaggg ccgcgaaaac
     2341 gggcggcgcg ccgcttcagc ccccggtcac cgacatcggc cgcgccacgg aaggccgatg
     2401 ctcgcggccg atgacgaagt catggccctt gggcttccgg gcgatggcgg catcgatcgc
     2461 ggcatgcaga agggcgtcat cgctgcttgc tctgagcggc gtcctgagat cggcggcgtc
     2521 ctcctggccg agacacatat agagagtgcc ggtgcaggtg acgcgcacgc gattgcacga
     2581 ctcgcagaaa ttgtgcgtca tgggcgtgat gaagccgagc ctgcctcccg tctcgcgcac
     2641 cttgacatag cgggcggggc cgccggaccg gtaggggtcg tcgtcaaggg tgaaatgctg
     2701 cgcaagccgc gcgcggacca gcgacagcgg cagatattgc gccgtgcggt cggcgccgat
     2761 ttcgcccatg ggcatggtct cgatcaaagt gagatcgaag cccctgccat gggcccagcg
     2821 caggatctcc tcgatctcgt cctcgttcac gtcccgaagc gccaccatgt tgatcttgat
     2881 cttgaggccg gcacgcgcgg cggcgtcgat gccgcccatc acttgggcga ggtcgccccg
     2941 gcgcgtgatg gcccggaact tggcggcgtc gagcgagtcg agggacgcat tgatgcgggc
     3001 gacgccgctt gcggctagag cctcggcgtg aagggcaagc tggctgccgt tggtggtgag
     3061 cgtcaactcg tcgagaccat tgccgagatg gcgcgagagc gagcggatca gccacatgat
     3121 gttgcgccgg acgagaggct cgccgccggt gagcctcagc ttgcgaacgc ccttggcgac
     3181 aaacgcgctc gccagccggt cgatctcctc cagcgtcagc agctcctcgc gcgggaggaa
     3241 cgtcatggcc tcggccatgc aatagacgca gcgaaaatcg cagcggtcgg tgaccgaaac
     3301 ccgcaaatag gtgatgcggc ggccgaaggg gtcgatcagg gggcttccgg tcatacttgt
     3361 tcgctgtctt cttgttcgat ccgaaccttg cccttgcgtc tataggcctt ggcgttcttc
     3421 acaacccatt tgcgataaac ggggctcgcg agcgctgcgg cggcagcgct gcggcgcttt
     3481 accgtccttt tcgccttcgt cgttcgtccc gctctgcctt tcgggctgac gcgtcgcttc
     3541 ccggaggtga ccggcatagg cgttggtctc taatatgggc gtttcgggcc ttatatatgc
     3601 acttcgggtt tgtcataaca tgggatggag cgccatagga gaccaggcat ggacgagcaa
     3661 cgcttcaata tgtcgatgcg caagtatctc aaggaggtcg gcgtgacctc gcagcaggcc
     3721 atcgaggagg tggtgcgcga ggagcacctc ggcggcaagg gcaagctcaa gatgaagatg
     3781 gtgctcacgg ccgagggcac cagactcaag catgtgatcg acggcacgat cgacctcggc
     3841 tgacacccct tgcaaaaagg ccgtcgatga ccgagccatg gccgaccgag ctcaagctcg
     3901 acaaggggcg gcgcgtgctc accgtaacct atgacgacgg tgtgagcttt gcgctgccgg
     3961 ccgagttctt gcgcgtgctg tcgccgagcg ccgaggtgca aggccatagc cccgaacagc
     4021 gcgtcacggt cgcgggcaag aaatcggtcg gcatcgcccg gctcgagccc gtc
//
LOCUS       DASUQP010000220         3632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_244559,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000220 DASUQP010000000
VERSION     DASUQP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244559"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFY74_09950"
     CDS             complement(<1..416)
                     /locus_tag="VFY74_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932056.1"
                     /translation="MARSYFEQALANPSVPPNVAAQIRLYLQQLALAADPPAFSATIF
                     SAIRWESNANSGPGTQNVTLNGFDFTLDQNAVGHSGWSALNIGTLHYGYELKQGDRIE
                     LDALVYSTAYFDSKLQDIDLDFFELTLGPSFNMKRWG"
     gene            929..2494
                     /locus_tag="VFY74_09955"
     CDS             929..2494
                     /locus_tag="VFY74_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5932057.1"
                     /translation="MGEADSLQAEVLDFLGRPQAYVLPVATVERIETHASVVFLAGSF
                     AYKVKRAVKYNFLDFSTLEKRQVACLNELRVNLRTAPQLYLEVLPVIEDDGKFHLGGD
                     GEAIEWVLKMRRFDQAALYDDMAAEGRLSLAGMPRLAEIIAAFHESADRVLSAEQAVR
                     PLKGVLSDNAETFAAHPAIFPSQAARALDGASRDRLAALAPLLATRARGGYVRHCHGD
                     LHLRNIVEIDGAPVLFDAIEFDDRLATIDVLYDLAFLLMDLGKRGLNSHANAVLNAYL
                     DAEGSTGNLLGLAALPLFLSMRAAVRAKVELLRANLAAPDRAESAREEVHAYFALAQS
                     FLAPAGPSLIAIGGLSGSGKSAVSRAIAPHIGAFPGAVHVRSDVERKRLFGVAPLERL
                     PADAYTLEVTKRVYAMCRKRALLTLEGGQSVIVDAVHARPEEREALAALAADNGVPFT
                     GLWLDAPASILRDRIAARTGDASDATPELVDVQLGYEIGPQNFTPIDASGPVDRVAAS
                     CLERIGVEPRAPL"
     gene            2682..>3632
                     /locus_tag="VFY74_09960"
     CDS             2682..>3632
                     /locus_tag="VFY74_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5932058.1"
                     /translation="MAGPLLLGTALAFGLGGCGSGGNGQPLGERVIPFGQPVPKGGGY
                     YQIGKPYEVAGLWYTPREEPSYDRVGSASWYGELFHGRRTANGEIYDMDRLSAAHPTL
                     PLPIYARVTNLNNGRSLIVRVNDRGPFAHDRIIDLSRRSAELLGFRNQGTATVRVRYL
                     GRAPLSGDDSHELSYLANQSWARIATRRTTPNAASSTILVKERRPAEKTENPALAWNA
                     VKRERASPPTPQVTGAVPSNVTERALAAGLMVQAGTFKNKDNAERARTTLSAIAPVDV
                     ASISFRQELYFCVKVGPFSDLIEAKAALARVTKAGYPGAIV"
BASE COUNT          620 a         1160 c         1187 g          665 t
ORIGIN      
        1 ccccatcgct tcatgttgaa gctgggtccg agcgtcagct cgaagaaatc gaggtcgata
       61 tcctgcagct tggaatcgaa ataggcggtg ctgtaaacga gcgcatcgag ctcgatgcgg
      121 tcgccctgtt tcagttcgta gccgtaatgg agcgtgccga tattgagcgc gctccagccc
      181 gaatgaccga ccgcgttctg gtcgagggtg aagtcgaacc cgttcagcgt cacgttctgc
      241 gtgccgggtc ccgaattggc gttgctctcc cagcggatcg ccgagaagat ggtggcagag
      301 aaggccggcg gatcggcggc aagcgcgagc tgctgaagat agaggcggat ctgggcggcg
      361 acgttcggcg gtacgctcgg attggcaagg gcctgctcga aataggagcg ggccacctcg
      421 taagcgccga ggcgatagta gaggatgcca agttcgagct ggagccgcgc gtgttgggcg
      481 catagatcaa catgcgctcg agcgccgaga tcgcgccttc atagtcgccg acttgcgcgg
      541 agaggttcgc atattcgaac gccacatcga gatccgaggg gttcgaaagc atgcgtttga
      601 gaagagcttc gcggcgggcc tcgatctcgg gcgggccgag gcgctgtacg gcgacagtgg
      661 tgctgccctc ggcgaaaacc ggcgacgctt cgcccatggc gagaccggca accgcgagcg
      721 cgatcgccca tttcgtccac cccagcatca cgtgaccgtc tcggcctttc cgagaggcgc
      781 gtgcaggttg attcccgcca aatccgcccc gtccgccaca aagttaccga gttcaccgtt
      841 ggccagcaag ggccacgccg agtttcttga ggtttttcaa aggccctgtg gcccatttgg
      901 cacgcgattt gcgtgctatg gataatccat gggagaggcc gattcccttc aggccgaagt
      961 gctggatttc ctgggccggc ctcaagccta tgttcttccg gtcgcaaccg tagagcgcat
     1021 cgagacccac gcgtcggtcg tgttcctggc tgggagcttt gcctacaagg tcaagcgggc
     1081 ggtcaaatac aactttctcg acttttcgac gctggaaaag aggcaagtgg cctgcctgaa
     1141 cgagctacgc gtcaatttac ggaccgcgcc tcagctttac ctcgaagttc taccggtaat
     1201 cgaagatgac ggcaagtttc acctgggcgg cgatggcgag gccatcgagt gggtgctgaa
     1261 gatgcgccga ttcgatcaag cggcgctgta tgacgacatg gccgccgaag gccgactcag
     1321 tctggcaggc atgccccgcc ttgccgaaat catcgccgcc tttcacgaat cggccgaccg
     1381 ggtgctgtcg gccgaacagg ccgtccgccc gctcaagggt gtgctcagcg acaatgccga
     1441 gacctttgcc gcgcaccccg cgatcttccc gtcgcaagcg gcgcgggcgc ttgacggtgc
     1501 aagccgggac cggctcgccg cgcttgcgcc tttgctcgcg acacgcgcgc gcggcggcta
     1561 cgtgcgccat tgtcacgggg acctccattt gcgcaacatc gtggagatcg atggcgcccc
     1621 cgtgctgttc gacgcgatcg agttcgatga ccggctggca acgatcgacg tcctctacga
     1681 tctcgccttc cttctgatgg atctcggcaa gcgcgggctg aactcccatg ccaacgccgt
     1741 gctcaacgcc tatctcgatg ccgaggggag caccggcaat ctcttggggc ttgcggcgct
     1801 gccgctgttc ctttccatgc gggcggcggt cagggccaag gtcgagttat tgcgcgcgaa
     1861 cctggcggcg cctgatcggg ctgagtccgc gcgcgaggag gtccacgcct atttcgcgct
     1921 cgcgcagagt tttctcgcgc cggccggccc aagcctcatc gccatcggcg gactgtcggg
     1981 aagcggcaag tcggccgtct cccgcgcaat cgcgcctcac atcggcgcct ttcccggggc
     2041 ggtgcatgtg cgaagcgacg tcgagcgcaa gcgattgttc ggcgtggctc cgctcgaaag
     2101 gctccctgcg gacgcctaca cgctcgaagt cacgaagcgc gtctacgcca tgtgccgcaa
     2161 gcgcgcgctg cttacgctgg aagggggcca gtccgtgatc gtcgatgccg tccacgcgcg
     2221 gcccgaggag cgcgaagcgc ttgccgcgct tgccgcagac aacggcgttc cgttcacagg
     2281 actttggctc gatgccccgg cgtcgatcct gcgcgatcgc atcgcggcaa ggacggggga
     2341 tgcctccgac gccacgcctg agctggtcga tgtgcagctc ggctacgaga tcgggccgca
     2401 gaactttacg ccgatcgatg cgagtggtcc ggtcgaccgg gttgccgcct cctgcctcga
     2461 acggatcggg gttgagcccc gcgcgcccct ctagattcat cctttgctaa cggtctttgg
     2521 ccgacagtcg aacgagggcc ggccggccgc gatcgccggc ccagcgaggc gtttcgcgcg
     2581 cgcctttcta gcggcccgtt cgggggttct tgacgttgcg tcgggtcggt gagcctgcgc
     2641 cggaggcggc caggcatgtt tgtgacgtag gcttgctcgg cttggccggg ccgcttttgc
     2701 tcggcaccgc tcttgccttt gggcttggag gttgcgggtc ggggggcaac ggccagccgc
     2761 tcggcgagcg cgtcattccg ttcggccagc ccgtgcccaa aggcgggggc tattaccaaa
     2821 tcggcaagcc gtatgaagtg gcgggacttt ggtacacccc gcgcgaggag ccgtcttacg
     2881 accgcgtcgg cagcgcctcc tggtacggag aactgttcca tggacgccgc accgcgaatg
     2941 gcgagatcta cgatatggat cgcctctccg cggcgcaccc gacgctgccg cttcccatct
     3001 atgcacgggt gacgaacctc aataacggcc gctcgctcat cgtccgcgtc aacgatcggg
     3061 ggcctttcgc gcacgaccgt atcatcgacc tgtcgcgccg ctcggcggag ttgctcggct
     3121 tccgcaacca gggcacggcc acggtgcgcg tcaggtatct cggccgcgcg cccttgagcg
     3181 gcgacgacag ccacgagctg agttatcttg caaatcagag ctgggcaagg atcgccacga
     3241 gacgaaccac gccgaacgcc gcttcgagca cgatcctggt caaagaaagg cgccccgcgg
     3301 agaagacgga aaacccggcg ctcgcgtgga atgcggtcaa acgcgagcgg gcctcgccgc
     3361 caacgcctca agtcacgggc gccgtccctt ccaatgtcac ggaaagagcg ctcgccgcgg
     3421 gactcatggt ccaggcgggc acgttcaaga acaaggacaa cgccgagcgc gcgcggacga
     3481 cgctttcagc cattgcgccc gttgacgtgg cctcgatttc tttccgccaa gagctctatt
     3541 tctgcgtcaa ggtcggaccg ttttccgacc taatcgaggc caaggcggcc ttggcgcggg
     3601 tgaccaaggc gggatatcca ggcgccatcg tg
//
LOCUS       DASUQP010000221         6942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1198202, whole genome shotgun sequence.
ACCESSION   DASUQP010000221 DASUQP010000000
VERSION     DASUQP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198202"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..231)
                     /locus_tag="VFY74_09965"
                     /pseudo
     CDS             complement(1..231)
                     /locus_tag="VFY74_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
     gene            complement(934..1665)
                     /locus_tag="VFY74_09970"
                     /pseudo
     CDS             complement(934..1665)
                     /locus_tag="VFY74_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            1859..5047
                     /gene="putA"
                     /locus_tag="VFY74_09975"
     CDS             1859..5047
                     /gene="putA"
                     /locus_tag="VFY74_09975"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX5932059.1"
                     /translation="MEKPSGDLKPGAHLPSRFAVGEALLADEGKLVEDLIARARFSTE
                     QARGIEVLATRLVEAAREGSKTSGGVDSFLHEYGLSSEEGVLLLCLAEALLRIPDSET
                     ADRLIAETVGSGDWARHLDRSDSLLVNASTWGLMLTGRLLDWGEGEETDVTSVLQRLI
                     ARSGEPLIRNALRQAMRILGGQFVIGQTIDEALVNAGGEAAEGYRFSFDMLGEAARTA
                     NDAERYTERYEQAAAAIAAWAGPPARRSEDALIARPGLSVKLSALHPRYKPSQSARLR
                     SELVPRLAKLAHAMRDAWLPLTIDAEEADRLDLSLELLEPLFADPSLGGWNGLGLAVQ
                     AYSKRALPLIDWLGAVGSTTGRRIPVRLVKGAYWDSEIKWAQEAGLASYPVFTRKVNT
                     DVAYLAAARALLTSPHLFYPQFATHNAHTIAAIVTLAGNAPYEFQRLYGMGEALHREV
                     VRPERLGKPCRIYAPVGEHRDLLSYLVRRLLENGANTSFVNRLADDEAPIAAIIADPV
                     EKAAALSPKANPQIPLPPDLYRPERKNSLGLPLWERSAREPLLEAMRRALADQAEGNP
                     IVAGEESDSDGEVSQITSPHDSRVVVGVYRAAAPHAIDKALDKARAACDDWDRLGGEA
                     RAAVLDRAADVFEADRAGLMALLVREGGKTLADAQSDLREAVDHLRYAGCQARLKFSG
                     PRLLEGVTGEENTLSLHGRGVFAAISPWNFPLAIFTGQIAGALAAGNAVVAKPAEQTP
                     LVAARAIRLMHRAGVPAEVLHLLPGQGEVVGAALAKDPRVDGIAFTGSTETGLIINRQ
                     LAARDGPIAKLIAETGGLNAMIVDSSALPEQVTDDALASAFNSAGQRCSALRVLFLQD
                     DVADGMLDMLVDAARELKIGDPFDYATDVGPVIDAEARDALESHKARMRREARELLDL
                     PLGEEHRHGTYVSPAIYEISSIGVLTREVFGPILHVVRFSGDRLGEVCEAINATGYGL
                     TLGLHSRIKATATFVADRVRVGNIYVNRNQIGAVVGAQPFGGEGLSGTGPKTGGPHYL
                     EAFARERVCSTNLTASGGNASLLAAGSGEGSD"
     gene            5126..6676
                     /locus_tag="VFY74_09980"
     CDS             5126..6676
                     /locus_tag="VFY74_09980"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEX5932060.1"
                     /translation="MVAARDKSRQEREVELKLAFAPADAGRLAVSSALAAPVTPPEEH
                     ILVSTYFDTPDFALHRAGVYLRVRENAGRYTQTIKSAKTNAELLERFEWEQEIPRGIP
                     DLDAARGTALEPLLTPERRATIRPVFATRIRRKIFRFAQDGSEIEAAIDQGEIAASAR
                     TCAVSELELELKGGAKHALFVLARRFAETVPLRLEMKTKAERGYELVQDGGGKAEKAT
                     GIEIPPELAAGAAFQAIARNCLRQVVANEPATRAGEPESLHQMRIGLRRLRAAIAVFD
                     NIVGDDEAEKIKAELKWIARELGPARDLHVFAAEVLQPLRASRPSDAGLATANREIEK
                     SRSEAYSRAAAAIGSDRFRNFMLDLAAWIETGPWTEGKAGRTGTVAKHAKKELARLRK
                     RVKRKGADLRHLSESQRHRLRIRGKRLRYAAEFFAATFSGEGSDKRNAELVSALKDLQ
                     DGLGGLNDLATRRALITGGLAGSASEGAQLAASVGLDETAPQAEALLLKSEQAFARFA
                     AVKPFWMA"
     gene            complement(6691..>6942)
                     /locus_tag="VFY74_09985"
     CDS             complement(6691..>6942)
                     /locus_tag="VFY74_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX5932061.1"
                     /translation="CTVVVEAPHRHHHQGKLYSVRVDISVPGKDLIVGRAKPIDHAHE
                     DVYVAVRDAFVAVVRRLQDQTRKKRGSVTTHAARAAMEG"
BASE COUNT         1237 a         2242 c         2272 g         1191 t
ORIGIN      
        1 gaccccgccg tgaagcgccg tgccagacca ggtcatccgc tgcatccagg gcatcggcgc
       61 cccgctataa agattggagt ggtggcgccg gcgcttctcc aggatgctga agacgccggt
      121 cctggtgtcg tagccgcgca taccgctcga gatcttcgaa ctcgcgacga gcgcggtgcc
      181 ccgatagacc tcgagcttct gctgcccaag cgagacgacg agcatcagcg ggtccacgcg
      241 aacaggtggg gccatcgccg gcacctctgc gggcttctct ctgacacgaa gagcgatttt
      301 cggccgaacg gccgcgacga gaggcgattt ggcatcgctc aagacgtctc ttgcgggcgt
      361 cgcgagggca agtctttcga tcggagcgtc gttttcgctt ctttcaagcg gaacagcgcc
      421 cgccatcgtc ttttctggca ctggcgggat caggacgggc gcgacgtcgc cgattcgcaa
      481 tcgttgggcg gggacctgcc gggaaacaac gggcaagatg tccgaggcaa gcgcaggcgc
      541 atttcgcgca agcagcgttg gatgcaacga catggcagca agggatatcg gagcgatacg
      601 ccgcgccaca tttggcactt ccgcgcgcaa ttctatcgcg aggacggcgg gcgttagcga
      661 ggccaacgtc ggtgtgagcg atggtgccgt gccaacggtc ggagttgcct gaccgccgga
      721 atattgaagc ggccaaacaa ggcttacggg gaccgagacg gcgagcaggc cgatcgaaaa
      781 gagcgccgcg accaggagcg gcggctttcg gcgccgcgcc cggtcgccgg tgcccgatgc
      841 caacattggc tcgcgatagt ggagcggcag gaccgtcgaa tcgtcgatcc gggcttgtag
      901 gacttgcgtg ggggtcagat tcttggcgta gcgcaaatgc gcgccgcaca ggttcgcgtt
      961 gacaagattg gcgccggaga aatcggcgta atcgaagaaa gcgccgctca aattcgcgtc
     1021 cgcaaggtcg gcgacattga gccgagcata gcggagatcg gcgccttgca gatcggccgc
     1081 gccaagcgcg gctccggtca gggtcgcgaa gcgcaaactt gcggccttga gcttggcgtt
     1141 tgccagatcg gacccgaaaa aatctgcatg atcgagcagg gcaccgacca gatttgcgcc
     1201 tctgaggttg gcgcgatcga aagaggcaag ttgaagatcg gcgcgggaca ggtcggctgc
     1261 cgcaaggccc gcgtccgaca cggtcgcgaa gcggaaactc gcgccgctca aatttgcttc
     1321 agtgaagtcg gcgccggcga gacttgcgtg attgaatgtc gcttcgctga gatcaacggc
     1381 cttgagctcg gcctgatcca ggcgtgcata cctgaagtcg ctgccgtccg gttccgatcc
     1441 gaaccggcgg tcccacgcgc ttgaggcgag gctcgcgttg tcgcaagccg tgcgggagct
     1501 tgtcttggct tcggggttcg gttcggctcg tccgcggtgc gaagtttgtc gtttagtatc
     1561 agcgcgtccg gcccagcctg ctgacacatc ggtccaagac cgataggtgg ccgaccgcag
     1621 caggcggcca cgcttcgact gactggtcat ccccccgaga cccatctacg caactggtta
     1681 cgacgctacg aaccataggc gagaacttgg gcatgacgaa ggaggttccg ccgccgccat
     1741 ggaccgtgtt ttttgactct tcttgttgcc gaatgagcac atcgaatccc aagggggaat
     1801 tgttccggcc cctcgactgg catctcgaag accgcaaccc caggagatag attgggccat
     1861 ggaaaagccg agcggcgatc tcaagccggg tgctcatctt ccgtctcgct ttgccgtcgg
     1921 cgaggctctt ctcgccgacg aaggcaaact ggtcgaggac ttgatcgcgc gggcccgttt
     1981 cagcacggag caagcgcgcg gtatcgaggt cttggccacg cgcctggtcg aggcggcgcg
     2041 cgagggcagc aagacatcgg gcggggtcga ctctttcctg catgagtatg ggctctcgag
     2101 cgaggagggc gtgctgctgc tttgcctcgc agaagccctg ctgcgcattc ccgacagcga
     2161 aaccgcagac aggctgattg ccgagaccgt gggctcgggc gactgggcac gccatctcga
     2221 tcgctcggac tcgctcctgg tcaacgcctc gacctggggc ctgatgctga cggggcgcct
     2281 tctcgactgg ggggaagggg aagagaccga tgtgacgagc gtgcttcagc gcctgatcgc
     2341 aagaagcggc gagccgctca tccgtaacgc gctccgccag gcgatgcgca tcctgggcgg
     2401 ccagttcgta atcggccaga ccatcgacga ggcgctcgtc aacgctggcg gcgaagccgc
     2461 ggaaggatat cgcttctcct tcgacatgct gggcgaggcg gcgcgcaccg ccaatgacgc
     2521 cgagcgctac acggaacgct atgagcaggc cgccgcggcg atcgcggcct gggcgggccc
     2581 tcccgcgagg cgaagcgaag atgctctcat tgcgcgcccg ggcctctcgg tcaagctctc
     2641 ggcgcttcat ccgcgctata agccctctca gagcgcgcgc ctgagatccg aactcgtgcc
     2701 gcgccttgcg aaactcgcgc acgcaatgcg cgacgcctgg ctcccgctca ccatcgatgc
     2761 cgaggaagcc gaccggcttg atctttcgct cgaattgctg gagccgcttt ttgccgatcc
     2821 gtcgcttggc ggttggaacg ggctcgggct tgcggtgcaa gcctacagca agcgcgcgtt
     2881 accgctgatc gattggcttg gcgccgtggg gtcaacaacc gggcggcgca tccctgtgcg
     2941 cctggtcaag ggcgcctatt gggatagcga gatcaaatgg gcgcaggaag cggggcttgc
     3001 ctcctatccg gtgttcacgc gcaaggtgaa caccgacgtc gcctatctcg ccgcggctcg
     3061 cgccttgctg acgagcccgc atctcttcta tccgcaattc gccacgcaca atgcccacac
     3121 catcgcggcg atcgtcacgc tcgcgggcaa tgcgccttac gagttccagc gcctctacgg
     3181 catgggcgag gccctccacc gcgaagtcgt acgccccgag cgcctcggca agccgtgccg
     3241 catctatgcc cccgtcgggg aacatcgcga ccttctttct tatctcgtgc gccggctgct
     3301 cgagaacggc gccaacacgt ctttcgtcaa tcggctcgca gacgacgagg cccctatcgc
     3361 tgcgatcatc gccgaccccg tcgagaaggc ggccgccctc tcgccgaaag ccaaccctca
     3421 gattccgctt ccgcccgacc tctaccgtcc tgagcgcaag aacagcctcg gccttccgct
     3481 gtgggagaga tcggcgcgcg agccgctgct cgaggcgatg cggcgcgcgc ttgccgatca
     3541 ggccgagggc aatcccatcg tcgcgggcga ggagagcgac agcgacggcg aagtcagcca
     3601 gatcacgtcg ccacatgaca gtcgggtcgt ggtcggcgtc tatcgcgccg ccgctccaca
     3661 cgcgatcgac aaggcgctcg ataaggcaag agccgcctgc gacgattggg accggctggg
     3721 cggcgaggcg cgggccgcgg ttctcgatcg cgcggcagat gtgttcgagg ccgaccgcgc
     3781 gggcctgatg gcgcttctgg tgcgcgaagg cggcaagacg ctcgccgatg cacagagcga
     3841 cctccgcgag gcggtggacc atctccgcta cgccgggtgc caagcgcggc tgaaattctc
     3901 gggcccgcgc ctgctcgagg gcgtaacagg agaagagaac acgctcagcc tgcacgggcg
     3961 cggcgtcttt gcggcgatct cgccctggaa ctttccgctt gccattttca ccggccagat
     4021 tgccggcgcg ctcgccgccg gcaacgccgt ggtggcgaaa cccgcggagc agacgccgct
     4081 cgttgcggca cgcgcgatca gattgatgca ccgggccggc gttccggcgg aggtgctcca
     4141 tctcctgccg ggacagggtg aggtggtggg cgctgcgctc gccaaagacc ctcgcgtcga
     4201 cgggatcgcc ttcaccggca gcaccgagac cggcctcatc atcaatcgcc agcttgccgc
     4261 ccgtgacggc ccgatcgcca agctgatcgc cgagacgggt gggctgaacg ccatgatcgt
     4321 cgactcatcc gcgctgcctg agcaggttac cgatgatgcg ctcgcctccg ccttcaacag
     4381 cgccggccag cgctgttccg cgctccgcgt gctgttcctc caagatgacg tggccgatgg
     4441 gatgctcgac atgctggtcg atgcggcgcg cgagctcaag atcggcgacc cgttcgacta
     4501 tgcgaccgat gtcggcccgg tgatcgatgc ggaggcccgt gacgcgctcg aaagccacaa
     4561 ggcgcgcatg cgccgcgaag cgcgcgagtt gctcgacctg ccattaggcg aggagcatcg
     4621 gcacggcacc tatgtctctc ccgccatcta cgagatttcc tcgatcggcg tgctcacccg
     4681 cgaggtgttc ggccccatcc tgcatgtggt gcgcttttcc ggcgatcgcc tcggcgaagt
     4741 gtgcgaagcg atcaatgcca cgggctatgg tctcacgctt ggcctgcaca gccgcatcaa
     4801 ggcgaccgcc acgttcgtgg ccgatagggt cagggtggga aatatttatg tgaaccggaa
     4861 tcaaatcggc gcggtcgtcg gagcgcaacc tttcggcggc gagggcctct cggggacagg
     4921 cccgaagacc ggcggtccgc actatcttga agccttcgcc cgcgagcgcg tgtgctccac
     4981 caatctcaca gcctccggcg gcaatgcgag tctgctcgcg gccggaagcg gcgagggaag
     5041 cgactgacct ctctcccaca ttgcgcttga ttgtgacagc gagatatcgg ctctgtcggc
     5101 ccgccatggg gcaacggtag tcgtaatggt cgcagctcgc gataagtcgc gccaggaacg
     5161 cgaggtggaa ctcaagctcg cgttcgcgcc cgccgatgcc ggacggctcg ccgtcagttc
     5221 cgcgcttgcg gcacccgtca cgccgccgga agagcacatt ctcgtctcga cctatttcga
     5281 tacgcccgat ttcgccctgc accgggcagg cgtttatttg cgcgtgcgag agaacgccgg
     5341 ccggtacact cagaccatca aatctgccaa gacgaacgcc gagctgctcg agcgcttcga
     5401 gtgggaacag gagattcccc gcgggatccc cgatctcgac gcagcgcggg gcaccgcgct
     5461 cgaaccgctg cttacgccag agcgccgcgc cacgattcgg cccgtcttcg cgacgcgcat
     5521 caggcgcaag atcttccggt ttgcgcaaga cggctccgaa atcgaggccg ccatcgatca
     5581 gggtgagatc gccgcgagcg cgagaacgtg cgcggtgtct gaattggaac tcgagctgaa
     5641 gggcggcgca aagcacgcct tattcgtcct cgcccgtcgc tttgccgaga cagtgccgct
     5701 caggctcgaa atgaagacca aggccgagcg cggctacgag cttgtccaag acggtggcgg
     5761 aaaggctgaa aaggccacgg gcatcgaaat tccaccggag cttgccgccg gtgcagcgtt
     5821 ccaggccatt gctcgcaact gtctccggca ggtcgtcgcc aatgagccgg cgacccgtgc
     5881 cggcgagccc gagtccctgc accagatgcg catcggcctg agaaggcttc gcgctgccat
     5941 tgccgtgttt gacaacattg tcggcgacga tgaggcggag aagatcaagg ccgagctcaa
     6001 atggattgcg cgcgagcttg gtccggcgcg cgatctccac gtctttgccg ccgaggttct
     6061 ccaacccttg agggcaagtc ggccttccga cgcaggcctt gccacggcca accgcgagat
     6121 cgagaagagc cgcagcgaag cctactctcg cgccgcggcc gcaatcggtt cggaccgttt
     6181 tcgcaacttc atgctcgatc tcgcagcttg gatcgagacc gggccttgga cggaagggaa
     6241 agccggacga acagggacgg tcgccaaaca tgccaagaaa gagcttgcgc ggctccgcaa
     6301 gagggtcaag cgcaagggcg ccgatctcag gcatctaagc gagagccaaa ggcatcgctt
     6361 gcgcatcagg gggaaacgcc tccgctacgc tgccgagttc ttcgctgcca ccttttcagg
     6421 cgaggggagc gacaaacgga acgcggaact cgtttccgca ctcaaggatt tgcaggacgg
     6481 acttggcggc ctcaacgatc ttgcgacgcg ccgggcgctg attaccgggg ggctggcggg
     6541 cagcgcaagc gagggggcgc aattggcggc gagcgtcggt cttgacgaga ccgcgccgca
     6601 agccgaggcg cttctcctca aatctgagca ggccttcgcc cgatttgctg ccgtcaagcc
     6661 tttctggatg gcgtgaagct ctccgtcgtt tcagccctcc atggccgccc gcgcggcgtg
     6721 agtcgtaacg ctgccgcgtt tcttgcgcgt ctgatcctgc agccgccgta ccacggcgac
     6781 gaaggcgtcc cgcaccgcga catacacgtc ctcatgagcg tggtcgatgg gcttggcccg
     6841 gcccacgatt aggtctttcc ccggcacgct gatatcgacc cgcacgctat agagcttgcc
     6901 ttgatgatga tgacggtggg gcgcctccac cacgacggtg ca
//
LOCUS       DASUQP010000222         5987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1199161, whole genome shotgun sequence.
ACCESSION   DASUQP010000222 DASUQP010000000
VERSION     DASUQP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199161"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..853)
                     /locus_tag="VFY74_09990"
     CDS             complement(170..853)
                     /locus_tag="VFY74_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5932062.1"
                     /translation="MHPPGLTHRLPNAHPLPSPTARSNYPRNWRLGTVGTALLDLARH
                     LGLKAIGTCSATNMAMVERLGATAIDYRAGDFVAAVRGLTAGRMGGVGVDAAFDAIGG
                     AHFARSFACLAPGGLLVGYGAQTMAIGGASLASAALGLARLKLWNALSGLFGGRHAVF
                     YNIISRRSAHPEDFKADMVTLFDLVRDRAIHPVVVDRLPLAAAREVHIRIDAGGLGGK
                     IVLLPWPRA"
     gene            complement(835..1218)
                     /locus_tag="VFY74_09995"
     CDS             complement(835..1218)
                     /locus_tag="VFY74_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5932063.1"
                     /translation="MAWQHIRIARFGGPEVLELAEQPTIPDPGPGEVRIKVLAAGTGF
                     TDTFIRRGRYPDFKGPLPFTPGYELVGVVEETGPGVPAQLAGQLVADLCVVGGYAQYA
                     IRPARFLVPVPDGVDPSEAVCIPLA"
     gene            1278..2030
                     /locus_tag="VFY74_10000"
     CDS             1278..2030
                     /locus_tag="VFY74_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932064.1"
                     /translation="MRAQFTVLSTLFASVAIIAWTPPAPTQPNGTLPVTAKPGEWRYL
                     NADPLSTRYSLLDQITKDNFKDLKIVWRWKPAIGPAPSSLGGTAQSNGDPTLAIYKSE
                     ATPIMANGVLYESAGGQRVVAAIDAATGKQLWLWNGMDESGRDRKAPRRNAGVFRVRA
                     CVAIAAIAAISSGAFAAEPVTKDEAVAMVKKRRGHQVRGSRQGLCRHRQSVSPVRRPR
                     ARYRGVRRRWDDTGARRQQSARRNQPDRRQGR"
     gene            1945..2193
                     /locus_tag="VFY74_10005"
     CDS             1945..2193
                     /locus_tag="VFY74_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX5932065.1"
                     /translation="MCGADGTILAHGANKARVGTNQIDGRDVDGKAFIKERVELAKQN
                     PTFWHSYKWLNPVTKKVEPKQMYCERLDTTTVCAGIYQ"
     gene            complement(2243..2713)
                     /locus_tag="VFY74_10010"
     CDS             complement(2243..2713)
                     /locus_tag="VFY74_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX5932066.1"
                     /translation="MKWVTRERPVIDRIACPWLIARFIDKEPEFLFVPPDQVDRVATE
                     TGAIPFDVPGVELTHVGEGCSFDSFISKYGLDNDPAILTIAAIVRGADTDRPDLTPQS
                     AGLLAVSMGLRDLSPDDHEVLNHGFIIYDALYAWASHRRSETHSWPPKMGSEAA"
     gene            complement(2760..2987)
                     /locus_tag="VFY74_10015"
     CDS             complement(2760..2987)
                     /locus_tag="VFY74_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932067.1"
                     /translation="MAATRKEVTGTRDTVYDLVSIIYHALQGAETYGMYIADAEEVGD
                     TELARFFEEVQEEERRRADRAKQLLAARLGR"
     gene            3323..5446
                     /locus_tag="VFY74_10020"
     CDS             3323..5446
                     /locus_tag="VFY74_10020"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5932068.1"
                     /translation="MRITLFGLAAIAIIGAFIAFVGPLFISTDDLRDTLLAQVETATG
                     YRLRVSGPVKVALFPSLDFVAEDIGVAQGGEGEAPEMATAKELRFGLELSALFGGKVK
                     MTEVTLIDPVIALPSGKNAATAGAGEETKPQGETGAALAALKTLSLDKLHIENGRLIM
                     PPSGGAPGKRFEGLMLSASLPSIDAPLAFDASAVVDGKAMRASGSIGDLGRFLDGDAV
                     PVALKISAPATLAEGATLNGIATYKGGSFALSQFTLRAGETALAGSANYKGSLLTLHP
                     LTLNAGGNSLSGSAVADVSGAVPAINAAFTGQALNLDAVLSKQGGASSATGNDRAQQA
                     AWSDARIDFAPLKSVTAKIKLSAGQLTYNGIKIAQANLHATIAGGKLAASLPNFKLYE
                     GAGTLSVNVDASGQIPAQRIRLSLVNFDAYPFLDDAAGFQSIEGTGAISLDLATSGGS
                     QRAMVAGLSGSAKFDFTDGAIRGINIAKTLRSLSTGVLSGWQENAAEKTDFATLGASF
                     KIAKGQAETSDLRLSGPLVRMAGVGHVDLPAQTLKFRVNPQLVASLEGQGGKADLKGL
                     GVPVVISGPWASPSIYPDIEGILQDPVAAYEQLNRLGGGLVSLPGAAGAKSVSGIDDL
                     INNGKIAPDSLKNGAIGGIGQLLGAEAPAEPASPSDTTSQEPASESEAVVAPPKKSKK
                     REARVEPAYKPEEAAAQALQNFFGD"
BASE COUNT         1191 a         1878 c         1891 g         1027 t
ORIGIN      
        1 gcattcctgg actaacgcca cgcgacaggt attctttggg tcaaggctga ctatcggcca
       61 ggtgattcta tcatcagcct tgtctccgaa gaggtgcgat gcctgctaag gtcaggagga
      121 gacatgctcg gcgcctgtcc ctatgtccgc tatgtgccag acgcggaagt cacgcccggg
      181 gccagggtag cagcacgatc ttaccgccaa gtccgccggc atcgatgcga atatggacct
      241 cgcgggcggc cgcgagcggc agacggtcga caactacggg atggatggcg cggtcgcgca
      301 ctaagtcgaa gagtgtgacc atgtcggcct tgaaatcttc tgggtgcgct gaccggcgtg
      361 agatgatgtt gtagaacacg gcgtgacggc cgccgaagag gccgctcagt gcgttccaca
      421 gtttgagccg cgccaaaccg agtgccgctg acgccaagct tgcgccgcca attgccattg
      481 tctgggcacc gtagccgacg agtaggccgc cgggtgcgag gcaagcgaaa gaccgcgcaa
      541 aatgcgcgcc cccaatcgcg tcgaaggctg catcgacgcc gaccccgccc atgcgccctg
      601 cggtgagccc gcgtacagcc gccacaaaat cgccggcgcg atagtcgatc gccgttgcgc
      661 cgaggcgctc gaccatagcc atgttggtgg ccgagcaggt gccgatcgcc tttaggccga
      721 gatggcgcgc cagatcgagc agcgccgtgc cgaccgtgcc gaggcgccaa ttacgaggat
      781 agttgctccg cgcggtaggc gacggtagcg ggtgagcatt tggtaggcgg tgagttaggc
      841 cagggggatg cacaccgcct cggagggatc gacgccatcg gggaccggca cgagaaagcg
      901 cgcggggcgg atggcatatt gcgcgtatcc accgacgaca cagagatcag caaccaactg
      961 tccggcgagc tgggcaggca cgccgggtcc ggtctcctcg acgacgccaa caagttcgta
     1021 acccggggtg aacggcagtg gccccttgaa gtccgggtag cggccgcgcc ggatgaaggt
     1081 atcggtgaag ccggtgccgg cagcgagcac cttgatgcgg acctcgccag ggccggggtc
     1141 gggaattgtc ggctgttccg ccagctccag gacctccggg ccgccgaacc gagcgatacg
     1201 aatgtgttgc cacgccatgc ggcaggttat aagcccgcgc ttgtgtcgcg cgccagagac
     1261 gacgaggacg ggctgtgatg cgggcgcagt tcacggttct gagcaccctc ttcgcgtcgg
     1321 tcgccatcat cgcgtggacc cctcccgcac cgacccagcc gaatggcacc ctcccggtca
     1381 ccgcaaagcc cggcgagtgg cgctacctga acgcagatcc cctcagtacg cgctactcgc
     1441 tgctggacca gatcaccaaa gacaatttca aggatttgaa gatcgtctgg cggtggaaac
     1501 ctgcgattgg tccggcgccg tcatctctcg gcggcaccgc gcagagcaac ggcgatccga
     1561 cgctcgccat ttacaagagc gaagcgacgc ccatcatggc aaatggcgtc ttgtacgaat
     1621 ccgcaggcgg tcagcgcgtc gtcgctgcca tcgatgccgc gacggggaag caattgtggc
     1681 tctggaacgg catggacgaa agcggtcgcg accgtaaggc gccccgccga aacgcaggcg
     1741 tttttcgcgt gagagcttgc gttgcgattg ccgccatcgc cgcgatcagc agcggcgcgt
     1801 tcgcagccga gccggtgacc aaggacgaag ccgtggccat ggtcaaaaag cgtcgcggcc
     1861 atcaggtccg agggagccga caaggcttat gccgacatcg acaatcggtc agcccggttc
     1921 gtcgaccgcg agctcgatat cgtggtgtgc ggcgccgatg ggacgatact ggcgcacggc
     1981 gccaacaaag cgcgcgtcgg aaccaaccag atcgacggca gggacgttga cggcaaggcc
     2041 ttcatcaaag aacgcgtgga gctcgccaag caaaatccga ccttctggca ctcctataag
     2101 tggttgaacc cagtcaccaa gaaggtcgag ccgaagcaga tgtattgcga gcggctcgac
     2161 acgacgacag tctgcgccgg catatatcag taggttcgcc ggcgcgcggc gcaaaagaat
     2221 cacacccgcc gcgaccacgc ctttaagcgg cttcggaccc catcttcggc ggccaactgt
     2281 gggtctccga ccttcgatgc gaggcccagg cgtagagagc atcataaatt atgaagccgt
     2341 gattcagtac ctcgtgatcg tcggggctaa ggtcgcgcaa acccatggag acagccagga
     2401 gaccggccga ttgaggcgtg aggtcaggcc gatcggtatc agcgccgcga acgatggcgg
     2461 caattgtgag aatggcggga tcattgtcga gaccgtactt ggatataaag ctgtcaaaac
     2521 tgcagccctc gcccacatgg gtgagctcaa ctccaggcac atcaaagggg atggccccgg
     2581 tctctgtcgc gactcggtcc acctgatcag gcggtacgaa caggaattcg ggttccttgt
     2641 cgataaatct ggcgatgagc catgggcagg caatccggtc gatgaccgga cgttctcggg
     2701 taacccattt catgacatta gctcctaggc gaactccacg cgggcgggaa tattgaatat
     2761 cacctcccca gccgagcggc gagaagctgc ttcgcgcgat ctgcgcgtcg tcgctcctcc
     2821 tcctggacct cctcaaagaa cctggccaac tcggtgtccc ccacctcctc cgcatcggcg
     2881 atatacatgc cgtatgtttc tgcgccttgt agtgcatgat agatgatgct gaccaagtcg
     2941 tagacagtgt cccttgtccc cgtgacttcc ttgcgtgtag cagccatgga gttctcctag
     3001 atcgcagcta cgatgggata accgaccgca gtttatgctc ccccgtcacc gcgagcaaac
     3061 gcacccgcgg catgtctgga tcgggtcatt ggcaggcgtc gggacgccga ctgccgatgt
     3121 ccgttctacc cctcaggagg gacatgcgcg gccctagtcc caatgtccct tttgtgccga
     3181 gcggcgagaa gctgctgacc gactggccgg cgatggagac acgtccagcc cgcagcagca
     3241 ggcacattcg gcctcttatc cattatttta ggattggcgc gtaattcgtt aaccacacgg
     3301 aattccaagt ctggaggggc acgtgcggat tacgctcttc gggttagcgg ccatcgccat
     3361 catcggcgcc ttcatcgcct ttgtcggccc gctcttcatc tccaccgacg acctgcgtga
     3421 cacgctcttg gcgcaggtcg aaactgcgac gggctatcgc ctcagggtga gtggaccggt
     3481 caaggtcgca ctgtttcctt cgctcgattt cgtggccgag gatatcggcg tggcgcaagg
     3541 gggcgagggc gaggcgccgg agatggcaac cgccaaagag ctgcgcttcg ggcttgagct
     3601 ttccgcgttg ttcggcggca aggtcaaaat gaccgaggtc acgctgatcg atcccgtaat
     3661 cgcgcttccg agtgggaaaa atgcggccac ggccggcgcc ggcgaagaga ccaagcctca
     3721 gggagagacc ggcgcggcgc tcgcggcgct caagactctc tcgctcgaca agcttcacat
     3781 cgagaacggc aggctgatca tgccgccttc cggcggcgcc cccggcaagc ggttcgaggg
     3841 gctcatgctg agcgcctctc tgccgtcaat cgacgcgccg ctcgctttcg atgcaagcgc
     3901 ggtggtcgac ggcaaggcga tgcgggcctc aggctcgatc ggcgatctcg gcagattcct
     3961 cgacggcgac gcggtccccg tcgcgctgaa gatcagtgca cccgcaactc ttgcggaagg
     4021 ggcgacgctc aacggcatcg cgacctacaa aggcggaagt ttcgcgctca gccagttcac
     4081 gctgagagcc ggcgagacgg ccttggcagg tagcgcaaat tacaagggca gcctgctgac
     4141 gcttcatccg ctcacgctga atgcgggcgg caacagcctg tcgggttcgg cggtcgccga
     4201 cgtctcgggc gccgttcccg ccatcaacgc cgcgttcaca ggacaggcgt tgaacctcga
     4261 tgcggtgctg tcaaagcaag gcggagcgtc gtcggcaacc ggcaatgacc gcgcgcagca
     4321 ggcggcgtgg agcgatgcga gaatcgattt cgccccgctc aagagcgtca ccgcgaagat
     4381 caagctctcg gccggccagc tcacctataa cggcatcaaa atcgcgcaag cgaatttgca
     4441 cgccacgatc gccggcggca aactcgccgc gagcctcccg aacttcaagc tctacgaggg
     4501 cgccggcacg ctcagcgtca atgtcgacgc cagcggccag attcctgcac agaggattcg
     4561 cctgtcgctc gtgaatttcg acgcctaccc cttcctcgat gatgcggcgg gatttcagag
     4621 catcgaaggg acgggagcga tctcgctcga tcttgcgacg agcggcggga gccagcgcgc
     4681 catggtcgcg ggcttaagcg gctccgcgaa gttcgatttc accgatggtg ccatccgcgg
     4741 catcaacatc gccaagacgc tgcggagctt gagcaccggg gtcctttcgg gttggcagga
     4801 gaatgcggcg gagaagaccg acttcgccac gctcggcgcg agcttcaaga tcgccaaggg
     4861 ccaggccgag acctccgact tgcgcctttc cggcccgctg gtgcggatgg caggcgtggg
     4921 ccacgtcgac ctcccggcgc aaacgctcaa gttccgtgtg aacccgcagc tggtcgcaag
     4981 ccttgagggt caaggcggca aggccgacct caagggactc ggcgtgcccg tcgtcatctc
     5041 aggcccctgg gcaagtccct cgatctatcc ggacatcgag ggcattctgc aggatccggt
     5101 agcagcctac gagcagctca accggctggg cggcggcttg gtgtcgctgc ccggcgcggc
     5161 aggcgcgaaa agcgtctcag gcatcgacga cctgatcaac aacggcaaga ttgcccccga
     5221 ttcgctaaag aacggggcga tcggcggtat cggtcaattg ctcggcgccg aggcgcccgc
     5281 ggaaccggct tcaccgtccg atactaccag tcaggagccc gcgagcgaat cggaagccgt
     5341 cgtggcgccg ccgaagaaga gcaagaaacg cgaggcccgc gtcgagcctg cctacaagcc
     5401 cgaggaagcc gctgcccagg cgctccagaa cttcttcggc gactagcaaa agcgaatcgg
     5461 ctcacgacga gcgatgcggc tctgaaagtc ggcggccaaa gctcgctgcc ggaccggcac
     5521 gcaagcaaag ccccggccgg gaaggacccg accggggctg gcatttgccg gagagatagg
     5581 taagtagggc tatcccgtcg ttttagctaa ccgagcttca acggctcggc caccacggct
     5641 ttttcgtggt gcccccaaca gccgccaagc ccaatcgcag ccgaaataat gatcgcgcaa
     5701 gctattaaga gacgcatggc taatcccccg ttccccgtcg tttctagtta tcactataag
     5761 ccatcccgcg cagaaagctg tgacaattgg gacactctcg accgaaaaga tgaggggtcc
     5821 atgcctttgg ggccctatcc tatgtggcag aggatagtgg gcaaccgctt tgactttcct
     5881 caataatttt aactcgcgat gcgcctgacc cattcggccc tggcgagctg cgatcgcccg
     5941 ggagtactgg cgggcacttg agcgcctcga tgccggtctc tccaatc
//
LOCUS       DASUQP010000223        20059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_927192,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000223 DASUQP010000000
VERSION     DASUQP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 20059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20059
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927192"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1132)
                     /locus_tag="VFY74_10025"
     CDS             complement(194..1132)
                     /locus_tag="VFY74_10025"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HEX5932069.1"
                     /translation="MFRSSFVTAMMMAAVTAFSVALSGCGQFSPPYLKPLSPQTRALL
                     AEKGMTEKSPVLIRIFKAESELEVWKAKDNGRFYLFKTYPICSYSGVLGPKIEQGDRQ
                     APEGFYSVSLDQMNPRSKYHLSFDVGFPNTYDRAHGRTGKNIMVHGDCTSSGCYAMTD
                     AVVEEIFTLAREALQNGQEEIHIQAFPFRMTAANMAAHKEDKWYDFWKSLKEGYDYFE
                     ISRMPPQVAVCDKRYVINASFAGSARVNPTGSCPSYQKLPIPRAPATQEASAKPANKR
                     QSASLAKPLGSVLGLTFGPSKQGYSAFTLGPATPGR"
     gene            complement(1302..2255)
                     /locus_tag="VFY74_10030"
     CDS             complement(1302..2255)
                     /locus_tag="VFY74_10030"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5932070.1"
                     /translation="MRTYLDFEKPIAELESKVAELKALAGEQRSVSIAEELARLERKA
                     REALEETYSGLTPWQKTQVARHPERPHFRHYIDAIVEDFTPLAGDRYFAEDQAIVGGV
                     GRLEGRSVIVLGHEKGSDTEGRIKHNFGMARPEGYRKAVRLMEMADRFKLPVVSLVDT
                     AGAYPGIGAEERGQAEAIARSTDCCLGLGVPIVAVIIGEGGSGGAVAIATANRVFMLE
                     HAIYTVASPEAAASILWRDSARAMDAATNMKITAQDLLKLGVIDGIIAEPIGGAHRDG
                     SEVMRATGETIVKALAAYDHSTPGEIRKERREKFLSIGRPA"
     gene            complement(2381..3352)
                     /gene="xerD"
                     /locus_tag="VFY74_10035"
     CDS             complement(2381..3352)
                     /gene="xerD"
                     /locus_tag="VFY74_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX5932071.1"
                     /translation="MAARRSPRAKDASELALFLDMLTAERGAAAHTVEAYTRDLADFF
                     AFLAAKGKAAKSATPDHIRAYLAGLARKGLAPTSRARKLSSIRQFYRFLLGEGLRTDD
                     PSSAIDSPKLGRPLPKILSLAEVETLLETAKGASDKAEEGTTQRRALRLYAALETLYA
                     TGLRVSELMALPRNVLTADDRVLTIKGKGGRERLVPLNETAQAALSAHLAAVRADEKE
                     RKRTSPWLFPSSGGQHLTRQRFGQELKALALTAGIEPARVSPHVLRHAFASHLLERGA
                     DLRTVQQLLGHADISTTQIYTHVIEERLRRLVEQHHPLAQAHSTKRS"
     gene            complement(3402..3626)
                     /locus_tag="VFY74_10040"
     CDS             complement(3402..3626)
                     /locus_tag="VFY74_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932072.1"
                     /translation="MPTLFRFLLILGVIAGVITGSLYVLAEYFQPEPKEISKALRNVK
                     GRLDETTASTPSVDTDAPADDSSTAGRRAQ"
     gene            3815..4441
                     /locus_tag="VFY74_10045"
     CDS             3815..4441
                     /locus_tag="VFY74_10045"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5932073.1"
                     /translation="MSLTLTETKKDDRQERVTWIREALGPRSIVLIGLMGAGKTAVGR
                     RLANRLDLPFIDADTEIEAAAGGTISEIFVEHGEAYFRQGERKVIGRLLDSGPQVLAT
                     GGGAFMAPETRASIKAHGVSVWLRADLKVLLKRVGRRDNRPLLAAGDPGEVMKRLMEK
                     RYPVYAEADVTVESRDVPHDMMVGTVIDALAAKLGYIAKPVRRRKKAQ"
     gene            4441..5580
                     /gene="aroB"
                     /locus_tag="VFY74_10050"
     CDS             4441..5580
                     /gene="aroB"
                     /locus_tag="VFY74_10050"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5932074.1"
                     /translation="MEDGAAALARRETVIEVSLGARSYPIVIGERLLATAGQWIATTL
                     PGARCAVVSDTNVAALYLSPLKASLVEHGLYLGDAVVRPGEASKSFPVLESLCERLLE
                     LGVERSDCVIALGGGVVGDLAGFAASILRRGVRVVQMPTTLLAQVDSAIGGKTGIDTK
                     QGKNLIGTFHQPSLVLADVSLLATLSAREFRAGYAEVIKYGLIGDAPFFAWLEKNSAE
                     IFSNSGAARRQAVETGARAKAAIVAEDEREESGKRALLNLGHTFGHALEAYAGYSDRL
                     LHGEAIAIGMRLAFAYSVERGLCPAGDAARVTEHLVAVGLPTEIAAVPGPRPSPEALL
                     RLMAQDKKVKGGRLALVLAHGIGQAFVERDVSMPQLSDFLARACA"
     gene            complement(5603..5884)
                     /locus_tag="VFY74_10055"
     CDS             complement(5603..5884)
                     /locus_tag="VFY74_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX5932075.1"
                     /translation="MSLEQDIRHKLTKAFAPEALEVVNDSHRHAGHAGSPGTGESHFT
                     IKVVSAAFAGKSRLERHRMVNEVLAQELAGKVHALAIKALAPEERSSSA"
     gene            5982..6605
                     /locus_tag="VFY74_10060"
     CDS             5982..6605
                     /locus_tag="VFY74_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5932076.1"
                     /translation="MDFDSKFFDRLRVKPDQNRLLRDSCPACDRPGCKEPGLYPAPKG
                     RGLEGQYHRFCLDHVREYNKSYNYFAGLPDEAVVKHQKDDMVGHRPTWSLGVNSWARN
                     RKSGSGARPGGFAHRFAAYDPFGLFPERAAKTDVGTPERPLKRAERKCLRQLNLEDSA
                     TKADIKARFKELVKRLHPDHNKGDRTSEDKLREVIQAYNYLRQAGLA"
     gene            6691..7677
                     /gene="cobS"
                     /locus_tag="VFY74_10065"
     CDS             6691..7677
                     /gene="cobS"
                     /locus_tag="VFY74_10065"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEX5932077.1"
                     /translation="MTLETAHSPGLPDMSVSVRQVFGIDTDLEVPAYSRPDEHVPDVD
                     PDYLFNRDVTLAVLAGFKHNRRVMIQGYHGTGKSTHIEQVAARLNWPCMRINLDSHVS
                     RIDLVGKDAIVIRDGMQVTEFREGILPYALQTNTALCFDEYDAGRPDVMFVIQRVLEV
                     SGKLTLLDQSKVIRPHPAFRLFSTTNTIGLGDTSGLYHGTQQINQGQMDRWSIVVTLN
                     YLPHDDEVNIVLAKAKSWNTEAGRKTIGNMVRVAELTRNAFMNGDLSTVMSPRTVITW
                     AENAEIFADIGTAFALSFLNKCDELERGLIAEFYQRCFGEDLPQSSANVALS"
     gene            7680..9566
                     /gene="cobT"
                     /locus_tag="VFY74_10070"
     CDS             7680..9566
                     /gene="cobT"
                     /locus_tag="VFY74_10070"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX5932078.1"
                     /translation="MTRRKEAPLDPFKRAVTLTTRSIAADSAMEVVFTSEPPGLNGKI
                     VRLPEPSRVPTKSEIAVIRGHADAAALAVACHDPALHAALAPPGGEAREVFEAIEQAR
                     VEAIGARRMQGVAANLAARMEQRYERTRLAQATERSEAPLPDALALLIREKLTGQTPP
                     VKARAVVNLWRQWVEGRARSVLDRMPDLLLDQEAFGRLARELLAALNLADQLDHGSEE
                     KSAEETEDDANAADAEQDQSEGKQRAELSADSREEEVSSEQALDDSDDALADLLDRAD
                     QSKQPRRSQPWRPNLSVLDNPEAFGYKVFTSAYDEIVNAEELCDVDELDRLRAFLDKQ
                     LQALHGAVARLANRLQRRLLAQQNRGWDFDLDEGMIDAARLARVIVDPMHPLTFKQER
                     DMQFRDTVVTLLLDNSGSMRGRPIMVAACCADILARTLERCGVKVEILGFTTRAWKGG
                     HAREAWLNALKPPLPGRLNDLRHIVYKSADAPWRRSRRNLGLMMREGLLKENIDGEAL
                     AWAHSRLIARPEQRRILMMISDGAPVDDSTLSVNSGSYLERHLRQVIDEIETRSSVEL
                     LAIGIGHDVTRYYRRAVTVTDAEELAGVMTEKLAELFDEDIAWRQLYQTVPAAARAMR
                     RKLH"
     gene            9568..10647
                     /locus_tag="VFY74_10075"
     CDS             9568..10647
                     /locus_tag="VFY74_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX5932079.1"
                     /translation="MKRRVRRSWQVLPAIALGAAASVAVLPAHAEKPLAPVQRPTPIT
                     IAASPIDFDRENPSRKEFGKLIFRAGLNLYAKSAHFGGFSALAVDPSGTSLLAVTDAG
                     SWLRATLDYDGRKLKGLEDAVFGPILGADGKPLRDGSQRDSEGMTIADGDTRKGTAYL
                     SFERKHRIARYPFNTERFGPPAGPVALPGGTKALGANRGIEAITVIKAGRLKGTLVAF
                     AEGLASGAGNLQGWLVGGPAPGSLTLKRIGGFDVTDAASLPDGGIVVLERRFRFSEGV
                     KMRIRRIAAGELKKGAVIAGEVLLEANDSFNIDNMEAIAAHRAASGETVLTLMSDDNF
                     SPLQRTLIMQFTLRDTKPMAAGLNR"
     gene            complement(10644..10946)
                     /gene="rpmB"
                     /locus_tag="VFY74_10080"
     CDS             complement(10644..10946)
                     /gene="rpmB"
                     /locus_tag="VFY74_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX5932080.1"
                     /translation="MARRCELTGKAVLTGNNVSHAKRRTKRRFLPNLCNVTLESEALK
                     KRVRLTVSAHALRSVEHRGGLDAYLLKAGDDELSLRARRLKRDIAKAQSAQAQAAS"
     gene            11169..12041
                     /locus_tag="VFY74_10085"
     CDS             11169..12041
                     /locus_tag="VFY74_10085"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5932081.1"
                     /translation="MQEARHCLSLLAVAALTCGLSGNLPIGAAHAENSLSTASRITAV
                     YRVELAGFNLGDFRLTTTFRGDDYEMRGVGRFSVLQGLLYEWRGFTASRGRVTSVGPE
                     PAMYAFSYSDSAKMAERLRLTFSDGDVTGVSIVPNKRPSPRTIPVTKEQLEGVLDPMS
                     GAFLSAHSANPNGDLNVCNATLPVFDGRQRFDLVVTPKRSVTVKRTTPAGYGGPAVIC
                     KVKFIPIAGYHPDNPAVRLMAQSNEIEVWLVPVKGTQMYVPYRIVLPTLAGYGSAVVT
                     SLQVSGPRRASAEP"
     gene            12423..15128
                     /locus_tag="VFY74_10090"
     CDS             12423..15128
                     /locus_tag="VFY74_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX5932082.1"
                     /translation="MLGPTNTGKTHLAIERMLAHDVGIIGLPLRLLAREVYDKVVARA
                     GAAQVALITGEEKIKPPSPRFYVCTVEAMPLELEADFVAVDEIQLAADGERGHVFTDR
                     LFHARGFGETLLLGAATMRDVIRELLPGANLVSRPRLSQLAYAGDKKITRLPRRSAIV
                     GFSASDVYAIAELVRRQRGGAAVVLGALSPRTRNAQVALYQNGDVDFLVATDAIGMGL
                     NLDLDHVAFAGIRKFDGQNHRDLTPAELGQIAGRAGRHMNDGTFGVTGQIAPFEVELV
                     DRLENHAFDQVGVLQWRNRKLDYGSVERLKDSLRETPEHRRLTRSRMVDDVIALENVS
                     HDPELRDMAQGTAAVALLWDMCQIPDYRKISAASHAELVATLYRFVMSEEGKIPADWF
                     ANQVAQTDRSDGDLDTLANRLAQIRTWTFVSHQAQWLHDPEHWQGRTRAIEDSLSDAL
                     HERLTQRFVDRRTSVLMRRLRDKEEIAAEIAPDGNILVENHFVGRLEGFHFTPDNAGE
                     GIHGRATRHAAAHVLASELSVRAAAFCAAPDAAIALKPNGRIVWQGAEVGRLERGEAA
                     LKPRIQLIADEHLSAADRERLAQRLEAWLKAELGSKLSPLMLLSEASDVTGLARGLAY
                     QLTENLGVLRREGAAADIKALDQEARAQLRKYGVRFGAFNVYIPALLKPAAAELVLML
                     WALHAGRDHGIDSEALPLRPQQGLTSVEAGAAIPEPYWRAAGFHVAGSRAVRIDMLER
                     LSDLIRARTIFRQTEGVEAPAGATGDGGFRAVSELMSLVGCSGEEFASILKSLGFRRE
                     RRPLAPVQEAGSQAPDTAADAEAIAIDEIWRPGSKRRAARQEKVEGRKPRRPQRAKAP
                     RRERPERPSQRPTQRAEAERKLEHSPFAALAALKGRLTARLPEGS"
     gene            15138..15497
                     /locus_tag="VFY74_10095"
     CDS             15138..15497
                     /locus_tag="VFY74_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX5932083.1"
                     /translation="MQRLDKWLFCTRLVRTRTGAARLVEAGRVRINGARALKPSRLVQ
                     PGDILTATPLGRLFVVRVLAAVERRGPASLARALYEDLTPRERQGPDPGQDGDRRKGA
                     RPTKRDRRRIDALRGGA"
     gene            15646..15984
                     /gene="fdxA"
                     /locus_tag="VFY74_10100"
     CDS             15646..15984
                     /gene="fdxA"
                     /locus_tag="VFY74_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX5932084.1"
                     /translation="MTYIVNELCIKCKYTDCVEVCPVDCFYEGANMLVIHPDECIDCG
                     VCEPECPVDAIKPDTEPGLERWLELNRKFSETWPNITSKKPAPPDADAFVSEPDKFEK
                     YFSSEPGEGD"
     gene            16309..16875
                     /locus_tag="VFY74_10105"
     CDS             16309..16875
                     /locus_tag="VFY74_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX5932085.1"
                     /translation="MADKKKPQQRHGFRTNEYIVYPAHGVGRIVRIEEQEIAGAKLEL
                     FVINFEKDKMTLRVPTAKLASVGMRKLAEEKTVKKALGTLKGKARIKRTMWSRRAQEY
                     EAKINSGDLVSIAEVVRDLYRSEAQPEQSYSERQLYEAALDRMAREIAAVEKLDEASA
                     VAKIDDVLAKTARHAKIAAETEARTRAA"
     gene            complement(16954..18588)
                     /locus_tag="VFY74_10110"
     CDS             complement(16954..18588)
                     /locus_tag="VFY74_10110"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5932086.1"
                     /translation="MESFTRVFPLDWAALRSVLLVAAILVAVCLSLAVVLWQLRSHRE
                     KQKKFVAEKIALQFAGDMMTRSRGDGDRFALKESNSIKTLVAESVLALAAQDPRIAGR
                     AIDRLDSGKAAPSADIFAGIARAKRAEAEQASREAAAALRRQGVLTLAGDSETALDLF
                     RQAVALDPASVDNLLALALAHFRSDDLESVESVRSTAEEGGNGAHHDIAGMIDMFGGI
                     LHFRRGNIFQAMEKFADAKGLFAAQGDAKREVDALMALANAKLKAGDRDGALADFNQA
                     AALCTEHKYEFGFAQLYADLGLLLQGLGKSSEAEQMMIKSLTVADRLGETAISAIAAG
                     SLALLYRETHDLDRAEDMARQALRFEGRLERKDGMARAGLNLGTILFERARFDEAGVR
                     FAESLRLYEELGPPELAAQAMFNLANVHRAQKRTEEAETSYREAMALFLSVNDAAGVA
                     RASGNLGTLYLEAGRLGDAEEEFNVALDAAREAGDKRAIAMQMRNLAVLAHARGETGA
                     ACEGLRKSLALCVEIDARTEAVELKVLMGQIGCQQG"
     gene            complement(18621..19502)
                     /locus_tag="VFY74_10115"
     CDS             complement(18621..19502)
                     /locus_tag="VFY74_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5932087.1"
                     /translation="MVGGVARGVACAGVVCLAAFLSPLARACDLPPPETAKVAAVIDG
                     ETLKLADGRTVKLIGAKAPNPPLGWRGEDPWPFVEDARGALESLAANKAIELRFGGSR
                     TDRHGYLLAQVFVVTGESRLWLQDDLVGRGLARVYSLPDNRACTAKLLAREEEARARW
                     LGLWSSPVYRIEDALDVKRLGRLVHTYQLVEGTVLDVGKGSGRLYLNFAPDWRSDFTI
                     SIERKDAASFTSAGLDPAALAGKRLRVRGWLAWRNGPMIEATHPEQIELLPVGGGEGE
                     AKPPPGQRAIGYSRAMP"
     gene            19732..19808
                     /locus_tag="VFY74_10120"
     tRNA            19732..19808
                     /locus_tag="VFY74_10120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19766..19768,aa:Arg,seq:cct)
BASE COUNT         3571 a         6556 c         6402 g         3530 t
ORIGIN      
        1 gccttcacgc tcggacccgc aacgcccggc cgttagccaa aggactcagc cctgtggctt
       61 gttgggagtt tcaaggttcg tcacccccgg gcttgacccg ggggtccatt ccgttacgcc
      121 tcggcttggc ttcggggccg cggaatagat cgcggggagg cagcggtcgc tagtccctct
      181 tgtagtcctt ccgctaacgg ccgggcgttg cgggtccgag cgtgaaggcg ctgtagccct
      241 gcttcgacgg cccgaaggtc aggccgagaa cgctgccgag cggcttcgca agagaggccg
      301 actggcgctt gttcgcgggc ttggccgagg cctcttgcgt ggcaggggcg cgcgggatcg
      361 gcagcttctg atacgacggg caggacccgg tgggattgac ccttgcgcta ccggcaaagg
      421 atgcgttgat cacgtagcgc ttgtcgcaca cggcgacctg tggcggcatc cggctgatct
      481 cgaaatagtc gtagccttcc ttcaagctct tccagaaatc gtaccacttg tcctccttgt
      541 gcgcggccat gttggcggcg gtcatgcgga acggaaaggc ctggatgtgg atctcctcct
      601 gtccgttttg caatgcctcg cgcgcgagcg tgaagatctc ctcgaccacc gcgtcggtca
      661 tggcgtaaca gccggacgac gtgcagtcgc catgcaccat gatgttcttt ccggtacggc
      721 catgggcgcg atcgtaagtg ttggggaagc ccacatcgaa ggagaggtgg tatttgctcc
      781 tcggattcat ttgatcgagc gagacggaat agaatccttc cggtgcctgc cggtcgccct
      841 gctcgatctt gggaccgagc acacccgaat agctacagat cggataggtc ttgaagagat
      901 agaagcgccc gttgtccttg gccttccaca cttcgagctc ggattcggcc ttgaagatgc
      961 ggatgagaac gggcgacttt tcggtcatgc ccttctcggc gagaagggca cgggtttgcg
     1021 gcgaaagggg cttcaggtag ggcggcgaaa actggccgca gcccgaaagc gcgacggaga
     1081 aagccgtcac tgccgccatc atcatggccg tgacaaagct tgaacgaaac atacgcgagt
     1141 gccccttgcg ctactcggtc acaatcgacg gtgagaggtt aaatcgaggt tagcgcttct
     1201 tccggcgctc cccttctgct ccccctaatt ggcgccaagt ataagcctgt cccaacagag
     1261 gtggaccaaa aaaaatggcg ctttttatgg cgatttaggc ctcaggccgg ccgtccgatg
     1321 gacaggaatt tttcgcgtct ttccttgcga atctcgcccg gcgtggagtg gtcgtaggcg
     1381 gcaagcgcct tgacgatggt ctcccccgtc gcgcgcatca cctcgctccc gtcacgatgc
     1441 gccccgccga tcggctcggc gatgatgcca tcgatgacac cgagcttcag caggtcttgg
     1501 gcggtgatct tcatattggt cgcggcgtcc atcgcgcggg ccgaatcccg ccatagaatc
     1561 gaggctgcgg cctcgggcga agcgaccgtg tagatcgcat gctccagcat aaagacgcgg
     1621 ttggcggtgg cgatggcgac cgcaccgccc gagccgccct cgccgatgat gaccgcgacg
     1681 atcggcacgc cgaggcccaa gcagcaatcc gtcgagcggg cgatggcttc ggcctggccg
     1741 cgctcctcgg ctccgatccc gggataggcc ccggcggtat ccaccaggct caccaccgga
     1801 agcttgaagc ggtcggccat ctccatcagc cgcacggcct tgcgatagcc ttcggggcgg
     1861 gccatgccga aattgtgctt gatgcggccc tcggtgtccg agcccttctc gtgaccgagc
     1921 acaatcaccg agcggccctc gagccggccg acgccgccga cgatggcctg atcctcggcg
     1981 aaatagcggt cgccggcgag cggggtaaaa tcctcgacga tcgcatcgat gtaatggcgg
     2041 aaatgcgggc gctcggggtg acgggcgact tgcgtcttct gccacggggt caggcctgaa
     2101 taggtctcct ccaaggcctc acgcgccttc ctctcgagcc tggcgagttc ctccgcgatg
     2161 gagacggagc gctgctcgcc ggcgagcgcc ttcagctcgg cgaccttgct ttcaagttcg
     2221 gcaatcggct tttcgaaatc gagataggtg cgcatcaaaa tctgattatg tccttggaaa
     2281 ccctcgtccc gacgagtctt aaggcgacaa atgaggcaat ggcatgggtt gtaagtggcc
     2341 cttgggccgg ggcgctgtca accgggcgcc cgtgccaaac ctagctccgc ttggttgagt
     2401 gagcctgagc gagaggatga tgttgctcga ccaggcgtct cagccgctcc tcgatgacat
     2461 gggtatagat ctgcgtcgtc gagatgtcgg cgtggccgag gagctgctga accgtgcgca
     2521 gatcggcgcc gcgctccaga agatgactgg caaaagcgtg gcgcagcacg tgcggtgaca
     2581 cacgcgccgg ttcgatcccg gcggtaaggg caagcgcctt gagctcctga ccgaagcgct
     2641 ggcgggtgag atgctggccc ccgcttgacg ggaagagcca gggcgacgtt cgcttccgtt
     2701 ccttctcatc ggcgcggacc gcggcgaggt gagcgctcaa tgccgcttgc gccgtctcgt
     2761 tcaagggcac gagccgctcg cgcccgcctt tgcccttgat ggtgagcacc ctgtcgtccg
     2821 ccgtgagcac gttgcgcggt agcgccatca gttccgacac gcgcaagccg gtggcataaa
     2881 gcgtctcgag cgcggcatag agcctgagtg cgcgccgctg cgtcgtcccc tcctccgcct
     2941 tgtcgcttgc gcctttcgcg gtctcgagca gcgtctccac ctcggcaagc gagaggatct
     3001 tgggaagcgg gcgcccgagt ttcggactgt cgatggccga gcttggatcg tcggtgcgaa
     3061 gtccctcgcc gaggaggaag cggtagaact ggcggatgga agagagtttt cgcgcgcgag
     3121 aggtcggcgc gagccctttc cgcgcgagac cggcgagata ggcgcggatg tggtcgggcg
     3181 tggcgctctt cgcggccttt cctttcgcgg cgaggaaagc gaaaaaatcg gcaaggtcgc
     3241 gcgtataggc ttcgacggta tgagccgccg caccgcgctc ggcggtgagc atgtcgagga
     3301 agagcgcaag ctcggaagcg tcctttgcgc gaggcgagcg tcttgcggcc aagggtcaag
     3361 cctttccgtg gccgacaagc gctaagttcg gcggggaaag cttactgcgc tcggcgcccc
     3421 gcggtgctgc tgtcgtcggc gggggcgtcg gtatcgacgc ttggcgtcga agccgtggtc
     3481 tcgtcgagcc ggcccttgac gttgcgcaag gccttggaga tttctttagg ctccggctgg
     3541 aagtattcag cgagcacgta gaggcttcca gtgatgacac cggcgatcac ccctaaaatc
     3601 agcaggaaac gaaacagtgt cggcatcgag gcgctcgtgt tccgaacagt ctattcgtcg
     3661 cgaacagggc gtcattatga cccaaaaaca cggcgggcgc cttatcctcg gcatcatcat
     3721 ggacgattcg acatggcgct gtcagcatgg gaaattcgaa gcgagtcgtt tatctgccgc
     3781 ttccggcggc gggtgcggca cctaagacca ttgaatgagc ctgacactga ccgagaccaa
     3841 gaaggacgac cggcaggagc gcgtcacgtg gatacgcgag gcgctcgggc cgcgcagcat
     3901 cgtgctgatc gggctcatgg gagccggcaa gaccgcggtg ggcaggcggc ttgcgaaccg
     3961 cctcgacctg cccttcatcg acgccgacac cgaaatcgag gcggccgccg ggggcaccat
     4021 cagcgaaatc ttcgtcgagc acggcgaagc ctatttccgc cagggcgagc gcaaggtgat
     4081 cgggcggctg ctcgacagtg ggccgcaggt cctcgccacc ggcggcggcg ccttcatggc
     4141 tcccgagacg cgggccagta tcaaggcgca cggtgtttcg gtttggctca gggccgacct
     4201 caaagtgctg ctcaagcgcg tcggacggcg cgacaaccgg cctctgcttg ccgcaggcga
     4261 tccgggagaa gtgatgaagc ggctcatgga aaagcgttat cccgtctatg ccgaggccga
     4321 tgtcaccgtc gaaagccgcg acgtgcccca tgacatgatg gtggggaccg tgatcgacgc
     4381 cttggccgcg aagctcggct acatcgcgaa gccggtcagg cgccgcaaga aggcgcaatg
     4441 atggaagacg gcgcggcagc attggcgcgg cgcgagaccg tcatcgaggt ctcgcttggc
     4501 gcccgctcct atcccatcgt gatcggcgag cgattgctcg ccacggccgg gcaatggatc
     4561 gccacgaccc tgccgggcgc gcgctgcgcg gtcgtaagcg atacgaatgt cgccgcactt
     4621 tatctttccc ctctgaaagc gagcctggtc gagcacggcc tctatctcgg cgacgcggtg
     4681 gtgcggcccg gcgaagcgag caagagcttt cccgttctcg aatcgctctg cgaacgcctg
     4741 ctcgagcttg gcgtcgagcg cagcgactgt gtcattgctc tcggcggcgg cgtcgtgggc
     4801 gacctcgccg gtttcgccgc cagcatcctt cgacgcggcg tgcgggtggt gcagatgccg
     4861 acgacgcttc tcgcgcaggt cgattccgcc atcggcggca agaccggcat cgacaccaag
     4921 caaggcaaga acctgatcgg cacgttccat cagccgagcc tggtgctggc cgatgtgtcg
     4981 ctgctcgcga ctctcagtgc cagagaattc cgcgcaggct acgccgaggt gataaagtat
     5041 ggcctgatcg gcgatgcgcc gttcttcgcc tggctcgaga agaattctgc cgagatcttt
     5101 tccaactcag gcgccgcgcg ccggcaggct gtggaaacgg gtgcccgcgc caaggcggca
     5161 atcgtcgcgg aggatgagcg cgaggagagc ggaaagcgcg cgctcctcaa tctcggccac
     5221 accttcggcc atgcgctcga ggcctatgcc ggctactccg accggctctt gcacggcgag
     5281 gccatcgcga tcggcatgcg tctcgccttc gcctattcgg tcgaacgggg gctctgccct
     5341 gccggcgacg ccgcgcgcgt gacagagcac ctcgtcgcgg tcgggcttcc gaccgagatc
     5401 gccgcggtcc ccgggcctcg gccttcgccc gaggcgctgt tgcggctgat ggcgcaggac
     5461 aagaaggtga aaggcggcag gctcgcactc gtgctagccc atggtatcgg ccaagccttc
     5521 gtcgagcgcg acgtttccat gccgcagctc tccgattttc tcgcccgcgc ctgcgcctga
     5581 cgcggccgaa tcccgaacac ggctaggcgg acgaagatcg ctcctcgggc gcgagcgcct
     5641 tgatggcaag ggcgtggacc ttgccggcaa gctcctgggc tagaacctca ttgaccatgc
     5701 ggtggcgctc cagccgcgac ttgccggcga aggccgccga caccaccttg atggtgaaat
     5761 gactctcccc ggttccgggc gaaccggcat gtccggcatg gcgatgggag tcgttgacga
     5821 cctcgagcgc ctccggcgca aaggccttcg tcaacttatg cctgatatcc tgttctaacg
     5881 acatgagcgc tgcgctcgac gacaaccttc cagattatcc cttttagcca cactgagcca
     5941 tgcttgttgt gtccagcgcg ccttctcata atgccgattc catggacttc gactcaaagt
     6001 tcttcgatcg cctgcgggtc aagcccgacc agaaccgcct cttgcgcgac tcttgtcccg
     6061 cctgcgatcg gccgggctgc aaggaacccg gcctctaccc tgcgcccaag ggccgggggc
     6121 tcgaggggca ataccaccgg ttctgcctcg accacgtgcg ggaatacaac aagtcctaca
     6181 actactttgc cgggcttccc gacgaggcgg tcgtcaagca ccagaaggac gacatggtcg
     6241 gacaccgtcc gacctggtcg ctcggcgtga attcctgggc gcgcaatcga aagtccggca
     6301 gcggcgcgcg ccccggtggc ttcgcccacc gtttcgccgc ctacgatccc ttcggcctgt
     6361 tccccgagcg cgccgccaag accgatgtgg gcacgccgga gcggccgctc aagcgcgccg
     6421 agcggaagtg cctccgccag ctcaatctcg aggacagcgc caccaaggcc gatatcaagg
     6481 cgcgcttcaa ggagctggtg aagcggctcc atcccgacca caacaaaggc gaccggacct
     6541 cggaggacaa gttgcgcgaa gtgatccaag cctataacta tctgagacag gcagggcttg
     6601 cgtaacgtcg ctgggcggcc cgcttttccc cgatcagggc ttggctgcgg caagcccgca
     6661 agagatgcga ccaacaggct ttcaccgatc atgacactcg aaactgccca tagccccggc
     6721 ttgccggaca tgtcggtttc cgtgcgccaa gtcttcggca tcgacaccga tctcgaagtc
     6781 ccggcctatt cgcgtcccga cgagcacgtg cccgatgtcg atccggacta tctctttaac
     6841 cgcgacgtga cgctcgccgt ccttgccggc ttcaagcaca accgccgagt catgatccag
     6901 ggctatcacg gcaccggcaa atccacccat atcgaacagg tggcggcacg gctgaactgg
     6961 ccctgcatgc gcatcaatct cgacagccat gtgagccgca tcgacctcgt cggcaaggac
     7021 gccatcgtca tcagggacgg catgcaggtg accgagtttc gcgagggcat cctgccttac
     7081 gctctccaga ccaacacggc gctctgcttc gacgagtatg acgccggacg ccccgacgtg
     7141 atgttcgtga tccagcgcgt gctcgaggtc tccggcaagc tcactctgct cgaccagtcg
     7201 aaagtgatcc gacctcaccc cgccttccgc ctgttctcga ccaccaacac catcgggctc
     7261 ggcgatacga gcggcctcta tcacggcacc cagcagatca accagggcca gatggaccgc
     7321 tggtccatcg tcgtcaccct caactatctg cctcatgacg acgaggtgaa catcgtgctc
     7381 gccaaggcca agagctggaa caccgaggcg gggcggaaaa cgatcggcaa catggtccgt
     7441 gtggctgagc tcacccgcaa cgccttcatg aacggcgacc tgtcgacggt gatgagcccg
     7501 cgcacggtca tcacctgggc cgagaacgcg gaaatcttcg ccgatatcgg caccgccttc
     7561 gcgctctcct tcctcaacaa atgcgacgag ctggagcgcg ggctgatcgc cgagttctat
     7621 cagcgctgct tcggcgagga tctgccgcaa tcgagcgcca acgtggcctt gagctagtca
     7681 tgacacggcg taaggaagcg ccgctcgatc ctttcaagcg tgccgtcacg ctgacgaccc
     7741 gctccatcgc cgccgacagc gcgatggagg tcgtgttcac gagcgagccg ccgggactta
     7801 acggcaagat cgtgcggctg cccgaaccgt cgcgcgttcc caccaagtcg gagatcgcgg
     7861 tcatccgcgg ccatgccgat gccgcggcgc tcgccgtcgc ctgtcacgat ccggctctgc
     7921 acgccgcact ggcgccgccc ggtggggagg cgcgcgaagt gttcgaggcg atcgagcaag
     7981 cgcgcgtcga ggcgatcggc gcaagacgca tgcagggcgt ggcagccaat ctcgccgccc
     8041 gcatggaaca gcgctatgag cggacccggc tcgctcaggc gaccgagcgc tccgaagccc
     8101 cgctacccga tgcgcttgcc cttctgattc gcgagaagct caccggccag acgccgccgg
     8161 tcaaggcgcg cgccgtggtc aatctctggc ggcaatgggt cgagggccgg gcgcggagcg
     8221 tgctcgaccg catgccggac ctgcttctcg atcaggaggc gttcggaagg ctcgcacggg
     8281 aattgcttgc cgcgctcaat ctcgccgacc agctcgacca tggctccgag gaaaaatcgg
     8341 ccgaagagac cgaggacgac gccaacgccg cggacgccga gcaggatcaa tccgagggca
     8401 agcagcgtgc cgagctttcc gccgattcgc gcgaagagga ggtctcgagc gaacaggcgc
     8461 tcgacgacag cgatgacgca ttggcggatc ttctcgaccg cgcggaccag tccaagcagc
     8521 cgcgccgctc gcagccctgg cgcccgaatc tctcggtgct cgataacccc gaagccttcg
     8581 gctacaaggt cttcacgagc gcttatgacg agatcgtcaa tgccgaggaa ctgtgcgacg
     8641 tcgacgagct cgaccgattg cgcgccttcc tcgacaagca gcttcaagcc ctgcatggcg
     8701 ccgttgcccg tctcgccaac cgccttcagc gcaggctcct tgcccaacag aaccgcggct
     8761 gggatttcga tctcgacgag ggcatgatcg atgcggcacg gcttgcccgc gtcatcgtcg
     8821 atcccatgca tccccttacc ttcaagcagg agcgcgacat gcagtttcgc gacactgtcg
     8881 tcacgctcct gctcgacaat tccggctcga tgcgcgggcg cccgatcatg gtggcggcct
     8941 gctgcgccga tatccttgcg cgtacgctcg agcggtgcgg cgtcaaggtc gagatcctcg
     9001 gctttaccac gcgggcgtgg aagggcggac atgcgcgcga agcttggctg aacgcgctga
     9061 agcccccgct tcccgggcgg ctcaacgacc tccgccatat cgtctacaaa tccgccgacg
     9121 ctccgtggcg caggtcgcgg cgcaatctcg ggctgatgat gcgcgaaggc ttgctgaagg
     9181 agaatatcga cggcgaggcg ctcgcctggg cgcatagccg gctgatcgcg cggcccgagc
     9241 agcggcgcat cctgatgatg atctccgacg gcgccccggt cgacgattcc acgctttcgg
     9301 tcaactcggg gtcatatttg gagcgtcatc ttcgccaggt gatcgacgag atcgagaccc
     9361 gttcctccgt cgagctactc gccatcggca tcggccatga cgttacccgc tactatcgcc
     9421 gcgccgtcac cgtgaccgat gccgaggaac tcgccggcgt catgacggaa aagcttgctg
     9481 agctgttcga cgaggatatt gcctggcggc agctctacca gaccgtgcct gccgcggcgc
     9541 gagccatgcg gcggaagctt cattgacgtg aagcgtcgcg ttcgccgatc gtggcaagtc
     9601 ctgcccgcca tcgcgcttgg cgcggcggcg agtgttgccg ttcttccggc gcacgccgaa
     9661 aagccgctcg cgccggttca gcgcccgacg cccatcacga tcgccgcttc tcccatcgac
     9721 ttcgaccgcg agaatccttc gcgtaaggaa ttcggcaagc tcatcttccg ggctgggctt
     9781 aatctttacg ccaaatctgc tcatttcggc ggcttctcgg cccttgccgt cgacccttcc
     9841 gggacgtcgc ttcttgccgt taccgatgcc ggaagctggc tcagggcgac gctcgactat
     9901 gacggaagga agctgaaagg cttggaggat gccgtgttcg gtccgatcct tggcgccgac
     9961 ggaaagccgc tgcgcgacgg cagccagcgg gactccgaag gcatgacgat cgccgacggc
    10021 gacacgcgga agggcaccgc ctacctttcc ttcgaaagga aacacaggat cgcgcgctat
    10081 cccttcaaca ccgagcggtt cggaccgccg gcgggccccg tcgctttgcc gggtggaacc
    10141 aaggcgctcg gagccaatcg cggcatcgag gcgatcacgg tcatcaaggc tggacggttg
    10201 aagggcacgc tcgtcgcctt tgccgaaggc ctcgccagcg gtgccggcaa tctgcaaggc
    10261 tggctcgtcg gaggtcctgc tccgggaagt cttacactga aaagaatcgg cggcttcgac
    10321 gtcaccgatg ccgcttcgct gcccgatggc ggcatcgtgg tgctcgagcg gcgtttccgt
    10381 ttcagcgaag gcgtgaagat gcggattcgc cgcattgcag ccggcgagct caagaagggc
    10441 gcggtcatcg ccggcgaggt gctgcttgaa gccaatgaca gcttcaacat cgacaatatg
    10501 gaagcgatcg ccgctcaccg cgcggcgtcg ggggagaccg tgctgaccct catgtcagac
    10561 gacaatttca gcccgctgca gcggacgctg atcatgcagt tcacgctgcg tgacacgaag
    10621 ccgatggccg cgggcttgaa ccgctagctt gcggcttgag cttgggcgct ttgcgccttg
    10681 gcaatgtcgc gcttgagcct gcgcgcccgg agcgacagct cgtcgtcgcc cgccttgagg
    10741 agataggcgt caaggccgcc gcgatgctcg accgagcgca aggcatgggc gctgacggtg
    10801 agccgaacac gcttcttcag cgcttcgctc tccagcgtca cattgcaaag attcggcagg
    10861 aatcggcgct tggtccggcg cttggcatgg ctcacattgt tgccggtaag cacggctttg
    10921 ccggtgagct cgcaacgacg ggccatggtc cttattcctt cctcttcaat gcacggaacc
    10981 cggcgcgccg cttataaggg ttatcgtgag cggcagtgcc accggcggcg gagatatacg
    11041 gggcgggttg ttgggggtca aggcgccgcc acaaacttgc gccaacctcg caattgacga
    11101 aacccgcgaa tggcccgaaa ttctccgggg ggttcttgcg ggcgccccgc cttgctaagg
    11161 ttggagcgat gcaggaagcg cgccattgct tgagcctact ggccgtggcc gccctcacct
    11221 gcggtcttag cggaaatctt cccatcgggg ccgcccatgc cgagaacagc ctctccaccg
    11281 cgagccgtat tactgccgtc taccgggtag agcttgcggg cttcaatctc ggcgacttcc
    11341 gcctgacgac gacttttcgc ggcgatgact acgaaatgcg cggcgtgggc cgcttcagcg
    11401 tgctgcaagg ccttctttac gaatggcgcg ggttcacggc gagcagggga cgcgtgacca
    11461 gcgtcgggcc agagcctgcg atgtacgcgt tcagctattc cgatagcgcc aagatggccg
    11521 agcggctgcg gctgacattc agcgacggcg acgtcaccgg cgtctcgatc gttcccaaca
    11581 agcggccgag cccgcgcacc attccggtga ccaaggaaca gctcgaaggg gtgctcgacc
    11641 ccatgagcgg ggcgttcttg agcgctcatt ccgcgaaccc caacggcgac ctcaatgtct
    11701 gcaatgcgac gctgccggtt ttcgacggca ggcagcgctt cgacctcgta gtcacgccca
    11761 aacgctcggt cacggtcaag cggacgacgc cggcgggcta tggcggtccg gcggtgatct
    11821 gcaaagtcaa attcatcccc atcgccggat atcatcccga caatccggcc gtgcgtctga
    11881 tggcgcagtc caacgagatc gaagtctggc tcgtcccggt gaagggcaca cagatgtatg
    11941 tgccctaccg tattgtcttg ccgacgctgg cgggctatgg ctcggcggtc gtgacctcgc
    12001 tccaggtctc agggccgcgc cgcgcgagtg ccgaaccgta acggcgccga accgtaacgg
    12061 cgctcaaggg ttaacagaca ggccccagat cccaacatgt agtagggaac agatgtcgaa
    12121 gattaacctt cccctgattc gtgcgtgctc cgttcccgtt gctttcttca ggacttaaga
    12181 gagcgaaaag cccagtccgc ccgatgcggc gctcgcgact ttttcaggga acgcccaaga
    12241 ggccccaagg attgcgattc cgcccggaac gaaatccgaa tgtgcagagt cagcgattcg
    12301 gacgagcttc gttcccgcct cgttccgtgt ttctcaagac tcagagactt agatagtcaa
    12361 atatgacccg tccgttttcc ccgaattcca caggacccca gcgtgggcgc ggcgtcaccg
    12421 ccgtgctcgg gccgactaat accggcaaga cgcatctcgc catcgagcgc atgctcgccc
    12481 acgatgtcgg gatcatcggt ctccctttgc ggctgcttgc gcgggaggtc tacgacaagg
    12541 tcgttgcgcg cgccggcgcc gcgcaggtcg cgctcatcac cggtgaagag aagatcaagc
    12601 cgccctcccc gcgcttctat gtctgcaccg tggaggccat gccgctcgag ctcgaggccg
    12661 acttcgttgc ggtcgatgag atccagctcg ccgccgacgg cgagcgcggc catgtgttca
    12721 ccgaccgtct gttccacgcg cggggctttg gcgagacgct tctgctcggc gccgccacca
    12781 tgcgcgatgt catccgcgag ctcctgcccg gcgccaatct cgtgtcccgc ccgcgcctgt
    12841 cgcagctcgc ttatgccggc gacaagaaga tcacgcgcct gccccgccgg tcggccatcg
    12901 tcggcttctc agccagcgac gtctatgcca tcgccgagct cgtgcgccgc cagcgcggcg
    12961 gcgcggccgt cgtgcttggc gcgctcagcc cgcgcacgcg caatgcgcaa gtcgcgcttt
    13021 accagaacgg cgacgtcgat ttcctcgtcg cgaccgacgc catcggcatg gggctcaatc
    13081 tcgacctcga ccatgttgcc ttcgccggga ttcgcaaatt cgacggacaa aaccaccgcg
    13141 acctgacccc ggccgagctc ggccagatcg ccggccgcgc cggtcgccac atgaatgacg
    13201 gcacgttcgg cgtgaccggt cagatcgcgc cgttcgaggt cgaactcgtc gaccgcctgg
    13261 agaaccacgc cttcgaccag gttggcgtgc tgcaatggcg caatcgaaag ctcgattacg
    13321 gctcggtcga gcgtctgaag gacagcttgc gcgagacgcc cgagcatcgg cgcctgacgc
    13381 gctcacgcat ggtcgacgac gtgatcgcac tcgagaacgt gagccacgac cctgagctgc
    13441 gcgacatggc gcaagggacg gcggcggtcg cgctcctgtg ggacatgtgt cagatcccgg
    13501 attaccggaa gatctcggcc gcgagccatg ccgagctggt ggcgactctc tatcgctttg
    13561 tcatgagcga ggaaggcaag attccggccg actggttcgc caatcaggtg gcgcaaaccg
    13621 atcgcagcga cggcgatctc gacacgctcg caaaccggct cgcccagatc agaacctgga
    13681 ccttcgtgtc gcaccaagcg caatggctcc acgatcccga gcattggcag ggccgcacca
    13741 gggcgatcga ggactctctg tcggacgcgc tgcacgagcg cttgacccag cgtttcgtcg
    13801 accggcggac gagcgtgttg atgcgccgct tgcgcgacaa ggaggagatc gcagccgaga
    13861 tcgcgcccga cggcaacatc ctcgtcgaga accatttcgt cggacggctc gaaggtttcc
    13921 acttcacccc cgacaatgcc ggcgaaggga tccatggccg ggcgacgcgg cacgccgcgg
    13981 cccacgtgct cgcaagtgaa ctttcggtcc gcgccgccgc gttctgtgcc gcgccggacg
    14041 ccgcgattgc gctcaagccc aatggacgga tcgtctggca gggcgcggag gtcggccgtc
    14101 tggagcgggg cgaagccgcg ctcaagcccc gcatccagct gatcgccgac gagcaccttt
    14161 ccgccgccga tcgcgagcgt ctcgcccaac gtctcgaggc gtggctcaag gccgagctcg
    14221 ggtccaagct cagtccgctc atgctgctga gcgaagcctc cgacgtgacg ggcctcgccc
    14281 gcggtctcgc ctatcaattg acggagaatc tcggcgtgct ccgccgcgaa ggagccgccg
    14341 ccgatatcaa ggcactcgat caggaggccc gcgcccagct tcgcaaatac ggcgttcgct
    14401 tcggcgcctt caacgtctac attcccgcgc ttctcaagcc ggccgctgcc gaactcgtat
    14461 tgatgttatg ggcgcttcat gccggccggg atcatggtat cgacagcgaa gcgcttccct
    14521 tgcgcccgca gcagggcttg acctcggtcg aagcgggagc ggcgatcccg gagccttatt
    14581 ggcgggcggc tgggtttcat gtcgccggct cgcgcgccgt ccgcatcgac atgctggaac
    14641 ggctctcgga cctgatccgc gcgcgcacta tcttccggca gaccgagggt gtggaggcgc
    14701 cggcgggcgc gacgggtgat ggcggctttc gcgcggtgtc cgagctgatg tcgttggtcg
    14761 gctgctcggg ggaggaattc gcctcgatct tgaaatcgct cggtttcagg cgcgagaggc
    14821 gcccgcttgc gccggtccaa gaagcgggat ctcaggcgcc ggataccgcc gccgacgccg
    14881 aggcgatcgc tatcgacgag atctggcgtc caggcagcaa gcgtagagcg gcgcgccaag
    14941 aaaaggtcga gggccgcaag cctcggcgcc cgcagcgcgc caaggcgcca cgtcgcgagc
    15001 ggcccgagcg tccgtcccag cggcccaccc aacgcgcgga ggcagagcgg aagctcgaac
    15061 actcgccctt tgccgctctc gccgctctca aaggtcgcct gactgcgcgc ctgcccgaag
    15121 gaagctgacg cgcgaccatg cagcggctcg acaagtggct cttctgcaca cggctcgtca
    15181 ggacgcggac gggagcggcg cggctagtcg aggccggcag ggtccgtatc aacggcgccc
    15241 gcgcgctgaa accgagccgc ctcgttcagc ctggcgatat cctcaccgct accccgctcg
    15301 gaaggctctt cgtcgtgcgg gtgctcgccg cggtcgagcg ccggggcccg gcgagcctgg
    15361 cgcgggctct ctatgaggac ttgacccctc gagagcgcca agggccggac cccgggcagg
    15421 acggcgaccg gcggaagggc gcccgcccga ccaagcggga ccgccggcgc atcgacgccc
    15481 tacgtggcgg cgcttaacgg ccgaaatgga cctaagaccg cgccatcggt cgccttgtgc
    15541 cggtcgcccg attgttatag gcctgcggtt ccgcgggcga tctcgggcgg cccctcaagg
    15601 ggctggaaat tgggccgcta gaggccgtga acgtgtgtgg gacggatgac ttacatcgtc
    15661 aacgagctct gcatcaagtg caagtacaca gactgcgtcg aagtgtgccc ggtcgactgc
    15721 ttctacgaag gcgcgaatat gctggtgata caccccgacg aatgcatcga ttgcggggtc
    15781 tgcgagcccg aatgtccggt tgacgccatc aagcccgaca ccgagcccgg gcttgagcgc
    15841 tggctcgagc tgaaccggaa attctcggaa acgtggccga acatcaccag caagaagccg
    15901 gctccgcccg acgccgacgc ctttgtgtcg gaacccgaca agttcgagaa atacttctca
    15961 tccgagccgg gcgaaggaga ctaagtcctt caaagggtta aacgttaacc ctagggtcaa
    16021 aatgtcgcat gttgcggcgc aaaaacggcc ttgctgcggc acgggacggc ttcccgattt
    16081 agtaagattg tgttattgta gcggcaatgg cgcggattgc cggcgcaacc agggccaaga
    16141 gacgcgtccc tgtttgggcg cccttttttt tccgatttgg gggaggtggc gcgatcaggc
    16201 tcctgatcgg ctgctcatct tgtgcctagg ccccttcgcg aaaggtaaag ggccagttgg
    16261 attgtccggc agcgtcgcgt gccagttttt ggagagtgag acaagagtat ggctgacaaa
    16321 aagaaaccac agcaaagaca cggctttcgc accaacgagt acatcgtcta tccggcgcat
    16381 ggcgttggcc gcatcgtccg catcgaagag caagagattg ccggagccaa gctcgagttg
    16441 ttcgtcatca acttcgagaa ggacaagatg acgctcagag tgccgaccgc gaagctcgcg
    16501 agcgtcggca tgcgcaagct cgcggaagaa aagacggtga agaaggcgct tggcacgctg
    16561 aagggcaagg cgcgcatcaa gcgcaccatg tggtcccggc gcgcccagga atacgaggcc
    16621 aagatcaatt ccggcgacct cgtctccatc gccgaggtcg tgcgcgatct ttaccgttcg
    16681 gaggcgcagc ccgagcaatc ctattccgag cgccagctct atgaggccgc gctcgaccgc
    16741 atggcgcgcg agatcgcggc cgtcgagaag ctggacgagg cgagcgccgt cgccaagatc
    16801 gacgacgtgc tcgccaagac ggcgcggcac gccaagatcg cagccgagac cgaggcgcgg
    16861 acccgcgcgg cctaagacgc cctgaacttc agcggccgga attcatgccg ggcgcccgcg
    16921 ccgcggcgcg gtcttttctc gccaacgccg gcctcaacct tgttggcatc cgatctgacc
    16981 catcaggacc tttaactcaa ccgcctcggt tcgtgcgtcg atctcgacgc agagcgcgag
    17041 cgacttgcga agcccctcgc aggccgcgcc ggtctcgccg cgcgcgtggg cgagcacggc
    17101 gagatttcgc atctgcatgg cgatggcgcg cttgtcgccc gcttcgcgcg cggcatccaa
    17161 ggcgacgttg aattcctcct cggcgtcgcc gaggcggccg gcctcgagat aaagagtgcc
    17221 gagattgccg gaggccctgg ccacgcctgc cgcgtcgttc acgctcagga agagcgccat
    17281 ggcttcgcga tagctcgtct cggcttcttc ggtccgcttc tgggcgcggt gcacgttggc
    17341 gagattgaac atcgcctggg cggcgagttc gggaggaccg agttcctcgt aaaggcgaag
    17401 gctctcggcg aagcggacgc ccgcctcgtc gaagcgggct ctctcgaaca ggatggtgcc
    17461 gagattgagg ccggcccggg ccatcccgtc cttgcgctca agccgtccct cgaagcgaag
    17521 cgcctggcgg gccatgtcct ccgcgcggtc gaggtcgtgc gtctcgcgat agagcaaagc
    17581 gagactgccg gcggcaatgg cggagattgc cgtctcgccc agccggtcgg cgacggtcag
    17641 gctcttgatc atcatctgct cggcctcgga cgacttgccg agtccctgca gcaacaggcc
    17701 gagatcggcg tagagctggg caaagccgaa ctcatatttg tgctcggtgc agagcgccgc
    17761 ggcttgattg aaatcggcga gagccccgtc tcgatcgccc gctttcagct tcgcgttcgc
    17821 aagcgccatc agcgcgtcga cctcgcgttt cgcatcgccc tgcgccgcga agaggccctt
    17881 ggcgtcggcg aatttctcca tcgcctggaa gatgttgccg cgcctgaaat gcaggatgcc
    17941 gccgaacatg tcgatcatgc cggcgatgtc gtgatgcgcc ccgttccctc cttcctccgc
    18001 cgtcgatcgc acgctctcga cgctctccag gtcgtccgat ctgaaatgcg caagcgcgag
    18061 cgcgagcaag ttgtcgacgg aggcgggatc gagcgcgaca gcctgacgga agaggtcgag
    18121 cgccgtttca ctgtcgcccg ccagcgtgag cactccttga cggcggaggg ccgccgcggc
    18181 ctcgcgcgag gcttgctccg cctcggcgcg tttggcgcgc gcgatgccgg cgaagatgtc
    18241 ggcggaaggt gccgctttgc cggagtcgag ccgatcgatg gcgcgccccg caatccgcgg
    18301 gtcctgggcg gcaagggcaa ggaccgactc ggccaccagc gtcttgatgc tgttgctctc
    18361 cttgagcgcg aagcggtcgc cgtcccctcg cgaacgcgtc atcatgtcgc ccgcaaactg
    18421 caaggcaatc ttctcggcca cgaacttctt ttgtttttcc cggtggctgc gcagctgcca
    18481 caagacgacg gcaagggaaa ggcacaccgc gacgagaatc gcggcgacga gcaggacgct
    18541 tcgcaaggcg gcccagtcga gcggaaagac gcgggtgaag gattccatgg cgctcgatac
    18601 cccgttgcct cggttgcgaa ctatggcatc gcccggctat agccgatagc tctttgaccc
    18661 ggcgggggct ttgcttcccc ctcgccgccg cccacaggca gaagctcgat ctgctccgga
    18721 tgcgttgcct cgatcattgg accgttccgc caggcgagcc aaccccgcac gcgcagacgc
    18781 ttgccggcga gcgccgccgg atcgagcccg gcgcttgtaa aggaagcggc atctttccgc
    18841 tcgatgctga tggtgaaatc gctccgccag tccggagcga aattgagata gaggcggccg
    18901 ctccccttgc ccacgtcaag caccgttcct tcgaccagct gataggtatg aacgaggcgg
    18961 cccaggcgct tcacgtcgag cgcgtcctcg atgcgataga ccggcgagga ccaaagcccg
    19021 agccacctcg ccctcgcttc ttcttcgcgc gcgagaagtt ttgcggtgca ggcccggttg
    19081 tcgggaagcg aatagacccg cgccagtccc ctgccgacga gatcgtcctg gagccacagc
    19141 cggctttccc ccgtaacgac gaagacctga gcgagaagat agccgtgccg gtctgtgcgg
    19201 ctcccgccga accgaagttc gatcgccttg ttcgcggcaa ggctctcgag ggctcccctg
    19261 gcgtcctcga cgaacggcca gggatcctcg ccgcgccagc cgagcggcgg gttgggcgcc
    19321 ttggcgccga tcagcttcac cgttcggccg tcggcgagtt tcagggtctc gccgtcgatt
    19381 acggcggcga ctttggcggt ctcgggcgga ggcagatcgc atgcccgcgc gagcggcgag
    19441 agaaacgcgg cgaggcaaac cacgcccgca caagcaacgc ccctggccac tcccccgacc
    19501 atgacgcatc ttacggatgc gcgcttgggg agcacaagcg gggagcgacc ctatgctccc
    19561 ggctccgcta gcccaatcga aagggcgttg gacgaaatag aagcgctttt cggcaagtcc
    19621 ctatataaga ggccggaggc gcaacgcctc cgaacacgct gagaacatcc gcgggcaacg
    19681 cgctgacaac gtggacaacg gcgccgatgg caacctattg aaacgcaaca agaccccgta
    19741 gctcagcagg atagagcatc agattcctaa tctgagggtc acaggttcga atcctgtcgg
    19801 ggtcaccagt ctttgtttca ctaaatgcga ttcaatcact tagagccttg aacggtccgc
    19861 cgctactctt tgccgaccta ctcgcctgga cgtctcactt caacaggggc tctggaaccc
    19921 tttccgctcc cggtcgacgg aatgcatccc ggttcgttga cgccaacatg gcatggcttt
    19981 cgcacgccgc ccttgctatg aacgccttcg gtcgggtccc atccttcaag gcaccgaaaa
    20041 ttcccacggc actgatctg
//
LOCUS       DASUQP010000224        38008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2018732, whole genome shotgun sequence.
ACCESSION   DASUQP010000224 DASUQP010000000
VERSION     DASUQP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 38008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38008
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018732"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFY74_10125"
     CDS             <1..383
                     /locus_tag="VFY74_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932088.1"
                     /translation="SDGFVSLSYGPVDPAKTPVFFLSCFDAMGIAVLDLRQEIEGAKA
                     GDPLEIILSAGEAKAEVKGEAMRDEASGTAFAEASDIAVKPVLEVLRQTGPVTVTSGK
                     TSASLSDKGRNQAVERFSKDCPLD"
     gene            complement(387..2324)
                     /gene="dxs"
                     /locus_tag="VFY74_10130"
     CDS             complement(387..2324)
                     /gene="dxs"
                     /locus_tag="VFY74_10130"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5932089.1"
                     /translation="MSADSPTPLLDRIHEPADLRALADAELKQLAEELREELIAAVSV
                     TGGHLGAGLGVVELTIALHHVFDTPRDRLIWDVGHQSYPHKILTGRRSRIRTLRQAGG
                     LSGFCKRTESEYDTFGAGHSSTSISAGVGMAVARDLDGKSHNVIAVIGDGAMSAGMAY
                     EAMNNAGALDSRLIVILNDNDMSIAPPVGAMSAYLAKLISGGTYRRFRRFLKQLAHKI
                     LPKSWERRAARAEEFTRGFWTGGTLFEELGFYYVGPIDGHNFDHLLPVLRNVRAMKNG
                     PVLVHVVTKKGKGYAPAEVSADKFHGVGKFNVVTGAQETQVAGTPPSYTKVFAQSLIE
                     EARVDPKIVAITAAMPSGTGLDRFAQAFPERCFDVGIAEQHAVTFAAGLATEGFKPFA
                     AIYSTFLQRAYDQVVHDVAIQKLPVRFAIDRAGLVGADGPTHAGAFDLAFLSCLPGMV
                     VMAAADEVELMHMVATAAAIDDGPSAFRYPRGEGTGVLLPKRGTPLPIGRGRILREGS
                     AVALLSLGARLPECLTAADQLAGFGLPATVADARFAKPLDLDLLRLLAQNHEVLVTIE
                     EGSSGGFGAMALQALARDGLLDRGLKLRTLHLPDRFIEQNKPQAMYAEAGLDAASILA
                     AVFAALGKDRKAADAMRLAKA"
     gene            complement(2483..2740)
                     /locus_tag="VFY74_10135"
     CDS             complement(2483..2740)
                     /locus_tag="VFY74_10135"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5932090.1"
                     /translation="MAEPTDHSDIKSMSFEQALKELETIVDRLEKGDVELEASILIYE
                     RGEALKTHCDALLRKAEARVEKISLNQNGQPTGTEPLDVET"
     gene            complement(2772..3722)
                     /locus_tag="VFY74_10140"
     CDS             complement(2772..3722)
                     /locus_tag="VFY74_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX5932091.1"
                     /translation="MTTLLLTHPACLLHDTGYGHPERADRLRAIDDALAAPAFKTLKR
                     EEAPLADLAAIERLHPKAYVEMIRASIPARDHKFLDMDTVVSPGSWDAALRATGASLY
                     AVDQVMAGKADNAFCAVRPPGHHAEPSRAMGFCMFNSVAIAALHARAAHGAERVAVID
                     FDVHHGNGTQAAFWSDRDLFYGSTHQMPLFPGTGARDETGVGNIFNAPLKPGDDGDDF
                     RDAYERRIFPALEAFAPDFLLVSAGFDAHLKDPLANLRLLESDFAWITEKLLEAAEKH
                     CGGKLVSTLEGGYDLEALASSTAIHVQTLMGAAAGTQFSP"
     gene            complement(3745..3918)
                     /locus_tag="VFY74_10145"
     CDS             complement(3745..3918)
                     /locus_tag="VFY74_10145"
                     /inference="COORDINATES: protein motif:HMM:NF020542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HEX5932092.1"
                     /translation="MVRSILVKAAHDPEANVWFVEQSDLFGLNAEAETLEQLVEKLPA
                     VIADLLEPEEAGD"
     gene            4001..4951
                     /locus_tag="VFY74_10150"
     CDS             4001..4951
                     /locus_tag="VFY74_10150"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5932093.1"
                     /translation="MPIVPATPDAIRRAAQALARGEIVAFPTETVYGLGANALDARAV
                     AKVFAAKERPRFNPLIVHVSHLAEAGEYGLANGATRTLAEAFWPGPLTLVLKRRSTCA
                     VADLVSAGLDTIALRAPAHPVAQALLAKAALPIAAPSANRSGRLSPTAAAHVEAELGD
                     LPVMILDGGPSPLGIESTVVSVAGKEPALLRPGAIARDAIESVLGARLAAASGPERKT
                     SPGQQPRHYAPHTPLRLDAVTVRPDEALLAFGDNVPAHARATINLSPSGNLEEAAAKL
                     FAALRELDQAGVKTIAVMPIPATGLGEAINDRLRRAADRG"
     gene            4963..6402
                     /locus_tag="VFY74_10155"
     CDS             4963..6402
                     /locus_tag="VFY74_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5932094.1"
                     /translation="MKPAPKHTPPSPDTRDALVRIVGAEHAIRDEADMGPYLVEWRDR
                     YHGKAALVLKPADTGEVSSILKLANETRTAIVPQGGNTGLVGGQIPFESGHEVVLSLE
                     RLNRVRDIDLASNTMTVEAGLPLAQAQHVAASAGRLFPLSLASEGSAQIGGVLATNAG
                     GMAVLAYGSARELALGLEVVLADGEVWNGLKSLRKDNSGYDLKDLIIGSEGTLGIITA
                     AVLRLFPKIAEKVTCMAGLNALETAPAFFARAFARAGPALTAFELLPRIGIEFVLRHG
                     SGSRDPFPSSYQWYALFELTSPREGNELAPLAESLLAQGLEAGEIADAVVATSLSQGE
                     ALWRLRELMSEVQKEEGGSIKHDIAVPVARVPEFVARANRLVELMIPGARPVPFGHFG
                     DGNIHYNVSQPPGMERTVFLANWEALNAAVHEIVLDLGGSISAEHGIGRLKRDLLPHA
                     KDPLELALMRKIKAAFDPNLILNPGKLLR"
     gene            6399..7076
                     /locus_tag="VFY74_10160"
     CDS             6399..7076
                     /locus_tag="VFY74_10160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX5932095.1"
                     /translation="MKRLETDALWTEIDEYLTRSLVASDPVLDAALQATEAAALPAIN
                     VTPSQGKLLMLLARAIAARRILEIGTLGGYSTIWLARGLVPDGRLITIESNPDCAKVA
                     RANLAHAGLSGQVDVRVGRAQDVLPELAAEAPFDLVFIDADKPGTPGYFQWAVKLARR
                     GSLIVVDNVVRAGAILAAEGDDHVQGMRRFFELAASDPRVTATAVQTVGAKGHDGFAI
                     LLVTTAP"
     gene            complement(7087..7983)
                     /locus_tag="VFY74_10165"
     CDS             complement(7087..7983)
                     /locus_tag="VFY74_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX5932096.1"
                     /translation="MLPSEIIAHCRIDKGGDFKLADRDPSDTFGLAIEKEEADALLAA
                     GTKRLSKLQERLYAEGRWAVLAILQGPDTSGKDGVIKHVLSGINPQGCEVHSFKQPSA
                     LELAHDFLWRTTMALPARGHVGVYNRSYYEETIVVRVHPELLDKQNLPNRLVTDTIWK
                     ERYEDINNFELHLARSGTVPLKFFLHISKGEQSKRLLARIADPEKRWKFSFHDVEERK
                     LWDNFIKAYDDTIRHTATKHAPWYVVPADRKWFARLVVAATLVERMEALDLQFPKFDE
                     AQLGELKKAEAALLEEAKAPER"
     gene            complement(7998..8678)
                     /locus_tag="VFY74_10170"
     CDS             complement(7998..8678)
                     /locus_tag="VFY74_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX5932097.1"
                     /translation="MCSRYSLTSPPEAVRAYFGYRDTPNFPARFNIAPTQPIAVVHRD
                     REGLRRFRLMRWGLLPFFVKDVKRFPTLINARAEEALDKASFRHAMRYRRCLVPADGF
                     YEWTGPKGKRRPFLLRPHDARLIAFAGIYERWRDSKGDEMDTVAILTCPANSTVAPLH
                     DRMPVVLADRDFDAWLDVEGTAPEAARELLQPAPDDLFEAIELHPKINDSKREEPGIQ
                     EPLAPSLL"
     gene            8822..9253
                     /locus_tag="VFY74_10175"
     CDS             8822..9253
                     /locus_tag="VFY74_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5932098.1"
                     /translation="MNEHIPTDTGLAHCDGASMVVLNQNKVLLVRRGRAPFAGLWSLP
                     GGKIERGETPREAARRELKEETGVEADVEGVLDRVTVSAPGDATYRLTVFYGRPVAGA
                     LKAGGDAWTAEWVHLDEVDARPMTQGTAELIWTAAHRLRRS"
     gene            9294..10001
                     /locus_tag="VFY74_10180"
     CDS             9294..10001
                     /locus_tag="VFY74_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HEX5932099.1"
                     /translation="MGRGKRLRLRLVAGLLGAAWASMVLAPAAEAQFFWWFDQPKTAP
                     KPRAKARPPGDDLPAIVPRESTAPPPPDDRAYDDKLMRLAEILGAIHYLRELCGAEEG
                     QTWREQMKEILRNEGTTAVRRAKLVNAFNDGYRGYRRSYRTCTQSATLATNRFSTEGA
                     SIAAALAQSSTFASKGPPDAEPQASPGTNQDASAPASGEAGAGAQPAQGAGETGASAA
                     PVAPGGPPGPQEPEPPD"
     gene            complement(10141..11316)
                     /locus_tag="VFY74_10185"
     CDS             complement(10141..11316)
                     /locus_tag="VFY74_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5932100.1"
                     /translation="MSEQEDIAGLVRAVRGRVPMDAAQMLVKETPERIGAVLTELPQI
                     LVDRIKAYLPPELQLQASESLAEVVENTVGEIMDPALAVLPQTTTVEEAIAFLRNHET
                     PQQITYLYVTDADDKLVGLTVIRDLILAKPNQTLADVMLPEPFALTIDKDLADAVKAA
                     IHRHYPVYPVVDADRHVIGVVRGWRLFERQAIEISAQSGQMVGVDKEERLYTGIWTAF
                     KMRHPWLQVNLLTAFAAGFVVSLFEGTITQIVALAAFLPILAGQSGNTGCQALAITLR
                     GMTLGDLNSHPVRKLIAKELTLGAINGLLTGIVAGVAMWWSAGGSGNDQGLILGLVIL
                     FAMTGACMASGLFGVLVPLTLRRFGADPCTASSIFLTTGTDIAGMGLMLGLATVLVL"
     gene            11582..11842
                     /locus_tag="VFY74_10190"
     CDS             11582..11842
                     /locus_tag="VFY74_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932101.1"
                     /translation="MRSVRQVPRCSESAAHKQAALRYILDAWEEALHDGIEPEMLANA
                     ALFAALTDLIDVYGEDAVAMMTNGLSRRILHGEFTLTHPPLA"
     gene            complement(11967..12629)
                     /locus_tag="VFY74_10195"
     CDS             complement(11967..12629)
                     /locus_tag="VFY74_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX5932102.1"
                     /translation="MQSGAQIVLTTLQLVLAGIGIGVLVAAPIGPVNVLVIQRAVRGG
                     FWAGLAAGIGAVLGDGLLAAIAAFSVTAISNVMSTYSGQIQFVGGLLLIGFGLALLLA
                     KPAMTIPVGQKSHLLEHMGIIPQTFFLTVTNPGAVLGMAAMIGGLGSLIGGLNTYLEA
                     LLLVAAVMGGSLLWWLGLSELIATIRHKLTEGRLKLINRIAGAVLLAFGLLLLLDGLV
                     RG"
     gene            12777..13355
                     /locus_tag="VFY74_10200"
     CDS             12777..13355
                     /locus_tag="VFY74_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932103.1"
                     /translation="MMALAALVAGAAAVVYVHNEASNALAAQGAATVMAAGTADQAPK
                     ALYDFNALPDPVKRMIEAIAEAAQSGEIEKMRPVLESNELKPMIAADHVDDPIAFWKK
                     ESADGSGRDVLAAMLNVMSAGYVRVGKGQDEMYVWPYFAETGLSALTPAQEVELYRIV
                     PPQLAAPMKKSGKYSYYKLGIAPTGVWHYFLR"
     gene            complement(13340..14695)
                     /locus_tag="VFY74_10205"
     CDS             complement(13340..14695)
                     /locus_tag="VFY74_10205"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5932104.1"
                     /translation="MAETFDLVFKGGTVVNHAGEGHADIGVRDGRIAETGAIGEGMAA
                     RTIDATGLHILPGVIDSQVHFREPGLEHKEDLASGSRSAVLGGVTAVFEMPNTRPPTT
                     SADALTAKVRAASGRMHCDFAFFVGATQDNVGDLAELERLPGAAGVKVFLGSSTGDLL
                     VDDEATLARILATINRRASFHAENEARLRERIALQREGDPSSHYEWRDAEAALASTER
                     LLRLAAHAGKRVHVLHVSTAEEVELLALHKDFASVEVTPQHLTLAAPDAYERLGTKAQ
                     MNPPIRDRDHQDRLWWGIERGVVDVLGSDHAPHTLAEKAKSYPATPSGMPGVQTLVPV
                     MLDHVNAGRLSLARFVDLTSAGPQRVFGIAGKGRIAVGYDADLTIVDLKQERVVEDAW
                     IGSKCGWTPFAGRTLRGWPKGTLVRGRLAMWEGEIGEAKGEPVRFSEALPPAGAGYRR
                     K"
     gene            14765..15640
                     /locus_tag="VFY74_10210"
     CDS             14765..15640
                     /locus_tag="VFY74_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX5932105.1"
                     /translation="MSHCHASVLPSRAVLRIAGADAGNFLQGLITNDIGKAQGANGVH
                     AGLLSPQGKVLFDFFLVADGGGFLVDVAKEKAEDLAKRLAFYRLRATVEIGEDPSREV
                     AACWGGKPVQPTGGIVFADPRLPALGYRAVLAKGTSPTALGCDAATEQDYHDFRIGLG
                     VPEGGRDYAFGDVFPHEALFDQLSGVDFNKGCFVGQEVVSRMEHRSTARKRILPVEGA
                     AALPAPGTSIEAAGVAIGTLGSVSGSAGLALIRLDRVEEALARGETLAAGEVKIALRR
                     PAFARFAVPPMEVPA"
     gene            15637..16239
                     /locus_tag="VFY74_10215"
     CDS             15637..16239
                     /locus_tag="VFY74_10215"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX5932106.1"
                     /translation="MSAKPTRCPWPGTDPLYIAYHVEEWGVPERDDRALYEKLVLDGF
                     QAGLSWITILRKREAFRRAFDGFDPERVARYSMRRVEALMRDEGIVRNRMKIEGAIAS
                     ARAWLKIMETSAGFAPFLWDFVGGKPLVNRYRRISEIPAETELSRTISKELKARGFKF
                     CGPTIIYAFMQATGMVNDHLTGCPRHAACARLGATPPNLS"
     gene            complement(16226..16609)
                     /locus_tag="VFY74_10220"
     CDS             complement(16226..16609)
                     /locus_tag="VFY74_10220"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX5932107.1"
                     /translation="MLERLAKALTIFAIAATKKRTNLANAGIRRTAIVRVQADLICHW
                     EMHMAKLAKYTLSHDAKTKRWVLKNDGTGQTVKSFASKAGATKGGVLERAVGKEGSVK
                     IKKRNGKVQEERTYPRGRDPRVTKG"
     gene            16621..17253
                     /locus_tag="VFY74_10225"
     CDS             16621..17253
                     /locus_tag="VFY74_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEX5932108.1"
                     /translation="MRGTRIVQNAAASTSARAWQRMLSGRRLDLLDPSPLDIEIEDIA
                     HGLARVARWNGQTSGAHAFSVAEHSLLVELVAGELEPKLGRDFRLAALIHDAPEYVIG
                     DLISPFKAALSLDYRAFEAKLLAAIHVRFGLPSELPADVVKSIKRADKIAAYYEATGL
                     AGFSRAEARRFFGQPRGLSARLVARLSQLDPLPASRGEAAFLKRFNELHR"
     gene            17237..17872
                     /locus_tag="VFY74_10230"
     CDS             17237..17872
                     /locus_tag="VFY74_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEX5932109.1"
                     /translation="MSCIDDAIAAETAREKADERMNTLILATEAARVPPTMSPETIYV
                     APLSLVEATVADAKVSHLVTLINGDTAIPTPSGIGPDRHLRLAMNDICEPQPGLVLPC
                     VSHVGDLIRFTREWDRQAPLLIHCWAGISRSTAAAYIALCALNREGTEHELAWALRRA
                     SPTAYPNRLLVALADEALARDGRMIAAVEDIGRGQIAEEADVFSLRALLAA"
     gene            17876..18886
                     /locus_tag="VFY74_10235"
     CDS             17876..18886
                     /locus_tag="VFY74_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HEX5932110.1"
                     /translation="MAEPRRKPPPPQEARGGDPQIELGLNAAIVTVRERQPHILVVRS
                     GTAGSGAWDALPFGPFAPRAHRTLEIGLREWVKRQTGLDLGYVEQLYTFGDRGRHAEP
                     GGAHTLSVGYLALTRGAGEELLGSHWSPWYAYFPWEDWRQGRPDILTDEIEPRLKDWA
                     ERPPLPDEPARPLRRQERLRISFGIGGGWDEETVLERYELLYEAGLVAEARRDGRPAA
                     SQWPALPELGRPMEFDHRRILATALGRLRGKLKYRPVVFELLPPEFTLYELQKTVEAI
                     SGTLLHKQNFRRLVEQGGLVEATGNVSTATGGRPARLYRFRREVVLERPAPGLRVKAG
                     RG"
     gene            19021..20112
                     /locus_tag="VFY74_10240"
     CDS             19021..20112
                     /locus_tag="VFY74_10240"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5932111.1"
                     /translation="MVGALIAVSQQGASKERQIDSKQGPIKVERVAKGLKQPWGLAFL
                     PDGRMLVTERPGRLRIVTKDGALSEPLKGVPEVVARGQGGLLDVALDPAFAENGLVYL
                     SYSEPGEGGAGTAVARGKLGDGSLDDVEVVFRQKPKVDGGNHFGSRLAFSPDGKLFVT
                     LGDRFKFDPAQDLASHIGKIVRINPDGSVPDDNPFVGQEGKLPEIWSYGHRNAQGAAI
                     HPETGKLWESEFGPLGGDELNIPEAGKNYGWPVVSWGKHYDGTDIPDPTERPEFADAI
                     YHWNPVISPSGIAFYTGDAIPAWKGNLLIAGLSSEAIVRLELDGEKVAGEERIAMGTR
                     IRDVAQGPDGAVYALTDEGGEILKLTPAR"
     gene            20151..20549
                     /locus_tag="VFY74_10245"
     CDS             20151..20549
                     /locus_tag="VFY74_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932112.1"
                     /translation="MAQRGLSLGFFGVFGRSAELRELDKALRSVDLHPNLVPEAVKLT
                     AVSLLKEADREPEPAAYRAAAEIIAYCMVGSDAFASANDANLAAKVERRIEAALEPGT
                     TLDAKLVLLTLHARVIQPSVVEDFGLESGG"
     gene            20642..21568
                     /locus_tag="VFY74_10250"
     CDS             20642..21568
                     /locus_tag="VFY74_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5932113.1"
                     /translation="MSAIISIKNVSKTYATGFEALKSINLEIKKGEIFALLGPNGAGK
                     TTLINIICGIVTPSAGKVTIDGHDIVTDYRAARSLIGLVPQELTADMFETVWTTVSFS
                     RGLWSKWENPGHIESVLRSLSLWDRKDSKLMTLSGGMKRRVLIAKALSHEPTILFLDE
                     PTAGVDVELRKGMWDNVRSLRDSGVTVILTTHYIHEAEDMADRIGVINKGELILVEDK
                     AELMHKLGKKQLTLQLHQALDAIPAALAGHHLALANGGSELVYTYDTQGPRTGITGLL
                     TDLAAAGVRFKDLDTTHSSLEDIFVDLVRRER"
     gene            21565..22326
                     /locus_tag="VFY74_10255"
     CDS             21565..22326
                     /locus_tag="VFY74_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5932114.1"
                     /translation="MNFPAIVAIYKFEMARWGRTFLQSIVSPVIATSLYFVVFGAAIG
                     SRITEIEGIEYGVFIVPGLVMLTLLTQSVANASFGIYFPRFTGTIYELLSAPISAFEI
                     VIGYVGAAATKSILLGLVILATAWLFVPLKIAHPLWMVGFLVLTAVTFSLLGFIIGLW
                     ADGFEKLQLVPLLIITPLTFLGGTFYSISILPPFWRTVTLFNPVVYLVSGFRWSFYEI
                     ADVSPAVSLIMIGVFLAICLGVVWWIFKTGYRLKS"
     gene            22495..22788
                     /locus_tag="VFY74_10260"
     CDS             22495..22788
                     /locus_tag="VFY74_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932115.1"
                     /translation="MTTFDYTALAELYPARSWKRSAGRVTYKRFEVAAEAVRFAIEEL
                     PSEFLLGTYLEVEEERFDARQIRSLYESKDYPLPRGLAGSGEPKLKPASRKRK"
     gene            22818..23132
                     /locus_tag="VFY74_10265"
     CDS             22818..23132
                     /locus_tag="VFY74_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932116.1"
                     /translation="MQTYVAEIDGRAIFAFRAVDDSNAQAWLDVNAVMRHALETLHSN
                     GRRIWNGQAQIRARKARPHEATIWSNLSGEVAVADPGEETDSITVWLIPTSTGRHFRD
                     AP"
     gene            complement(23202..23411)
                     /locus_tag="VFY74_10270"
     CDS             complement(23202..23411)
                     /locus_tag="VFY74_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5932117.1"
                     /translation="MNKGTVKFYNAQKGFGFIEPEDGGKDVFVHATALERAGITQLIE
                     GQKVSFDTEQDSRNGKTAVGKIEIA"
     gene            23643..24980
                     /locus_tag="VFY74_10275"
     CDS             23643..24980
                     /locus_tag="VFY74_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5932118.1"
                     /translation="MRERSADKQGSAAPEAPQLRRTIGPTQMALYGLGSMLGAGIYGL
                     IGKAAGQAGNALWLAFVVAFVAALLTALSYASLGSRYPRAAGAAYVTERAYGFSLLSF
                     MVGIALVCSGLTSIATQSQVFAANIAALFGLEDMPLSWLALGFLLILTGIVFRGIRES
                     MWVNVLCALIEAGGLLLVIVVGASYWGSVDYLATPAVPGGDHAALLVMQATVLAFFAF
                     IGFEDMINVAEETREPERTVPLGLMLAMSGAAFLYIGVAVTAVSVVPWPKLAAAPGPI
                     TAVVERAAPSIPPIVFTVITLFAVANTALVNYVTASRLIYGMARQGLLPERLGSVHAA
                     RRTPHLAVAALFLVLAPLALLGTITELAAATVLLLLLVFMVVNGALFILKRRKSEKQG
                     RFEIPRAIPALGAMVCLVLIVVRVTTGDWRAPALAGALLFGALVLHVLMQMRR"
     gene            complement(24977..25144)
                     /locus_tag="VFY74_10280"
     CDS             complement(24977..25144)
                     /locus_tag="VFY74_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932119.1"
                     /translation="MQSQFMVGISVNGRGDRLVVKAEDALIAALKVKAERPEATITYV
                     RRLNRRGDQRF"
     gene            complement(25232..26773)
                     /locus_tag="VFY74_10285"
     CDS             complement(25232..26773)
                     /locus_tag="VFY74_10285"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5932120.1"
                     /translation="MPAPAEPAPQATTPADDAGHSEGTPPSTTTVQTVPIPAAATPIV
                     ASIRTKLADPAFRGKASAEDIAALEAFYNGRSEPVWMTDMGFSREGLAAIDEILEADN
                     WGLASGAFDLPSSSTLPPSLDEQARGEIELDLAILKYARHARGGRTDPDKLKNNDMVP
                     TFRDPKTVLAEIADAAEPALYLQSLHPKHEQFHSLRQALLKARAEAEAGAKPRNTQKI
                     LINMERWRWMPQDLGALYVQINIPEFMVYIVKNGKTIHSEKVVVGKPVYATPIFSADL
                     KSIVFNPEWTVPSTIIREDLLPRLRGGGGMFGSNTSVLKKHELNVNYNGKRVNPSSIN
                     WNTVNMGAISFTQAPGPKNVLGKVKFLYPNRHSVYMHDTIKRELLSLQVRAEGHHCPR
                     VANPGKVAAIILAEDSGVPQAEIDKLLAKGYNSGVAIRNRVPVHTTYFTLSADPEGKL
                     QSFGDLYGLDAGVAAAALGKQAKPEAVADNVEAKSEPKPKPKPSTVGSASPPPAPGFT
                     GSIPQ"
     gene            27231..28298
                     /gene="nadA"
                     /locus_tag="VFY74_10290"
     CDS             27231..28298
                     /gene="nadA"
                     /locus_tag="VFY74_10290"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX5932121.1"
                     /translation="MTRLQHSASHSARKTETALPPSLLPDLAYTPEVARETAHLYERV
                     ARVIPPIEWPGFAPYIKAINELKRARNAVVLAHNYMTPEIFHCVADVVGDSLQLAREA
                     AKTDAEIIVQGGVHFMAETSKLLNPDKKVLIPDLRAGCSLAASITGADVRALREAYPG
                     VPVVTYVNTSAEVKAESDICCTSANAVRVVESLGAKRVILIPDEYLAKWVATQTPVEI
                     ITWKGHCEVHERFTGEELRAYRESDPGVKIIAHPECAPDVLKEADFTGSTSGMIKWVK
                     EHQPSKVLLVTECSMSDNVAVEAPNVAFVRPCNLCPHMKRITLPKILESLVFLKEEVT
                     IDPRIAERARMSVERMINLNH"
     gene            28328..29959
                     /locus_tag="VFY74_10295"
     CDS             28328..29959
                     /locus_tag="VFY74_10295"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX5932122.1"
                     /translation="MTVHFEFSAKAHEGVVVIGAGLAGLFTALKLAPLPVTVVSAARF
                     GEGGSSLWAQGGIAAALAEGDTAEAHAADTIRAGAGIVDERIALLMAREARERIEDLL
                     GYGVPFDKDLEGHLALGREAAHGRNRILHVKGDTAGRAIMHALTEAVRKMPSITVLEG
                     LAARDLVLKHGSIVGIELAPADDPRASGFILPAGAVVLATGGAGQLYSVTTNPREARG
                     DGIAIAARAGAAIADAEFVQFHPTAIDIGRDPAPLATEALRGEGALLINARGERFMRA
                     VHDDAELGPRDVVARAVHREVMSGRGAFLDCRPLGTKLVQHFPTVVAACRAASIDPVR
                     EPIPVAPAAHYHMGGILTDASGRSTVDGLWACGEVASTGAHGANRLASNSLLEAVVFG
                     ARAAKDIASSWRPMSLKAVRQEPYRSVAAERGRAESAERKLRLAMTEGAGVIRDGTSL
                     RNALATIAALEKVYDPRLANMLTTAKLIAAAALERKESRGAHFRSDYPLSDERLARRS
                     FLTLEKAEAMAGEARETAAPARGRRSGLARAALPA"
     gene            29956..30822
                     /gene="nadC"
                     /locus_tag="VFY74_10300"
     CDS             29956..30822
                     /gene="nadC"
                     /locus_tag="VFY74_10300"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5932123.1"
                     /translation="MSAKSPFPLALRPLLVERAVLAALDEELGAAGDITTDATIAADA
                     TGEAAIVARQSGVVAGLDLAEAAFEALDPGVRFTRIVADGGRVAEGGKIATVAGKTRA
                     LLSAERTALNFLGRLSGIATLTASYVKAVEGTAARIACTRKTTPGLRALEKYAVRAGG
                     GINHRFGLYDAVLVKDNHIAAAGGLANALARLKSRAGHAVRIEVEVDTLDQLADALAY
                     PIDAVLLDNMDLDTLREAVKLAAGRVVTEASGGVTLETVREIALTGVDVISVGALTHS
                     PRNLDSSLEWQN"
     gene            complement(30773..31906)
                     /locus_tag="VFY74_10305"
     CDS             complement(30773..31906)
                     /locus_tag="VFY74_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX5932124.1"
                     /translation="MGRRLPRRMWHTVAALVPLPLIALGYVAAVPGNPVAFEVELYLA
                     GKGSDQSLTRRAEAAPERLPREAAPLFRAKGKEPRADQYLARNTVTSAGKQLIAASLT
                     GDPATTGAVSRRGEVNGAIKGDRLKPTTSVIALSNPASGPDTSLMLAAASTFDADSLR
                     PSPADIAYHPTPEALNFRYQGESQAEFEARERRCLAIAIYFEARGEPMRGQVAVGQVI
                     LNRVRSPLFPETICGVVYQGQMAPGCQFSFTCDGKTDNPRNDAQWARAQDIARRITKG
                     ELWLPEVGYSTYYHANYVRPNWTGDMSRIDTIGRHIFYKKRKEHPYLVEASAAPQPSE
                     PQPPESPPSLFTLAPAVSLVSAVAEDDSSSATPAMSLGFAASE"
     gene            complement(32175..34889)
                     /gene="ppdK"
                     /locus_tag="VFY74_10310"
     CDS             complement(32175..34889)
                     /gene="ppdK"
                     /locus_tag="VFY74_10310"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX5932125.1"
                     /translation="MDHAMTKAEPALKTSPKWVYSFGGGRADGSAAGRDRLGGKGAYL
                     AEMSRLGLPVPPGFTISAELCAVYYELGRKLPEELKPMVNAALDKVGEIVGARFGDED
                     NPLLVSVRSGSRASMPGMMDTVLNLGLNDRTVEGLARRSGDRRFAYDTYRRFIQMYAD
                     VVLGIDHELFEEILENVKNLKGFELDTELSAEDWAELIARFKTLVATELGTPFPQDIH
                     AQLWGAIAAVFGSWQNARAIAYRRLHDIPDDWGTAVTIQAMVFGNMGERSATGVVFTR
                     NPSTGARELYGEFLVNAQGQDVVAGIRTPQSLTEAARRAANASRPSLEALMPDVFATL
                     EAACAELESHFRDIQDIEFTVQEGKLFILQSRSGKRSTQAALKIAVDMAEEGLITREE
                     AVRSIDAGQLDQLLHPTLDPDAEKTVLAKGLPASPGAASGELVFDADEAVHLKAQGHT
                     VILARVETSPEDVQGMHAAVGILTTRGGMTSHAAVVARGMGRPCVVGASSVVIDLERE
                     TLSAGGVLLHKGQVVTIDGSTGQIIKGRVPMRQAELSDDFNTLMQWADGFRRLKVRAN
                     ADTPADARQARAFGAEGIGLCRTEHMFFEETRILAMREMILAEDPDGRRVALAKLLPA
                     QRQDFIELFEIMAGLPVTIRLLDPPLHEFLPHSEDIIAEVAASMGVSVARLRRRIAAL
                     TEFNPMLGQRGSRLLVSYPEIVEMQARAIFEAAIEASQALHDRVIPEIMVPLVVTKGE
                     LEIVKGGIASVASAVEKERGTNLAYSVGTMVELPRSCLMAGEIAESAEFFSFGTNDLT
                     QTTFGLSRDDAGRFLGEYVDKGIIQHDPFVTLDKAVGELMQIAAERGRRVRPDVKMGI
                     CGEHGGDPRTIGFCEKIRLNYVSCSPYRVPVARLAAAQAALGATVTDP"
     gene            complement(34952..35398)
                     /locus_tag="VFY74_10315"
     CDS             complement(34952..35398)
                     /locus_tag="VFY74_10315"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX5932126.1"
                     /translation="MPDHLFVYGTLRAESSHPMAHRLRVAARNIGRGSTPGRLYDFGS
                     WPGAFFAPEQKYRVIGNVFVLRPASRFLIDLDKYEGVTPAQGEEREWPEAEGLFHRIA
                     VKVTLDKGGSVEAWAYALKEMPRARLIGTGDFIADRRFRAPSGVRP"
     gene            complement(35842..37893)
                     /gene="glyS"
                     /locus_tag="VFY74_10320"
     CDS             complement(35842..37893)
                     /gene="glyS"
                     /locus_tag="VFY74_10320"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5932127.1"
                     /translation="MAELLLELFSEEIPARMQVRARADLARLLAQALSGAGLEYKELR
                     TFATPRRLVAVVDGLPARSPAVREEKKGPRVDAPETAIKGFLKSAGLASIDQAERRED
                     KKGAFYVATIEKRGRATAEVVAEIVPAIVQTFPWPKSMRWGSGKLRWVRPLHSMLCLL
                     DGKVVPFAVDGIRSGKETRGHRFMAPKPFAVKGFADYVKKLRAAKVILDGEERAKLIL
                     DGARALARNEKLTLVEDEGLLQENVGLTEWPVPLMGRFDEAFLSVPPEVLATAMKAHQ
                     KCFSLRKGEKLASRFIVVSNLKAKDGGKAIVAGNERVIRARLADAKFFFDQDRKVPLE
                     ERVPKLDAIIFHEKLGSQHDRIERVRGLARKLAPLVGADPDLAERAAILAKADLLSGM
                     VGEFPELQGVMGRYYALDQEEDPAVANAIAAHYKPLGPSDEVPREPVAIAVALADKLD
                     ALMGFWAIDEKPTGSKDPYALRRAALGVIRILLENGVRLPLMAQFRQQARGDANLNEA
                     AARNLLCFFADRLKVHLREQGARHDLVDAVFALGGDDLLMIVRRVDALGRFLDTEDGE
                     HLLTGTKRAINILRIEEKKDGIAYDQAPDSALLKRPEEKALAEAVNEVERAAPSAIAR
                     EDFESAMSEMAKLRVPVDAFFDFVTVNTQEPDLRANRLRLLNRIRATTLTVADFSKIE
                     G"
     gene            complement(37886..>38008)
                     /locus_tag="VFY74_10325"
     CDS             complement(37886..>38008)
                     /locus_tag="VFY74_10325"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX5932128.1"
                     /translation="LDARGVISVTERQAYILRVRDLAKACCAAWLKTEAGGADG"
BASE COUNT         6622 a        12280 c        12267 g         6839 t
ORIGIN      
        1 cgagcgacgg atttgtgtcc ttgagttacg gaccggtcga tcccgccaag accccggtgt
       61 ttttcctctc ctgcttcgat gccatgggta ttgccgtgct cgacttgagg caggagatcg
      121 aaggcgccaa ggccggcgat cccctcgaga ttattctttc cgccggcgag gcgaaggcag
      181 aggtcaaggg cgaagcgatg cgcgacgaag ccagcggaac ggcttttgcc gaggcaagcg
      241 atatcgcggt caagcccgtg ctcgaggtct tgcgccaaac gggcccggtc accgtcacct
      301 cggggaaaac gagcgcctca ctatccgaca aaggccgcaa tcaagcggtc gagcgattca
      361 gcaaggattg cccgctggac taagcctcag gccttggcga ggcgcatcgc gtcggccgcc
      421 ttacgatcct tgccgagcgc ggcgaacacg gcggcgagga tggaagccgc atcgagcccg
      481 gcctcggcgt acatcgcttg cggcttgttc tgctcgatga agcggtccgg caggtgcagc
      541 gtccttaact tcagcccgcg atcgagaagc ccgtcccgcg ccagagcctg aagcgccatc
      601 gcgccgaagc cgccgctcga tccctcttcg atggtgacga gcacctcgtg gttctgcgcg
      661 agaaggcgca agagatcgag gtcgagaggc ttggcgaatc ttgcatcggc gacggtcgcc
      721 ggcaaaccga agcccgcgag ctgatcggca gcggttaggc actccggcag tctcgcgcca
      781 agcgacagca gagccacggc cgagccctcg cgcaagattc ggccccggcc gatgggaagc
      841 ggcgtgccgc gcttcggcaa caagaccccc gtcccctccc cgcgcggata gcggaaagcg
      901 gaggggccgt catcgattgc ggcggcggtc gcgaccatgt gcatgagctc gacctcgtca
      961 gcggccgcca tgaccaccat gcccggcagg catgacagga acgccaggtc gaacgcgccg
     1021 gcatgggtcg ggccgtcggc gccgacaagg cccgcgcggt cgatggcgaa gcggaccggc
     1081 agcttctgaa tggcgacgtc atgcaccacc tggtcatagg cgcgctggag aaatgtcgaa
     1141 tagatcgccg caaacggctt gaagccctcg gttgcgagcc ccgcggcgaa ggtcaccgcg
     1201 tgctgctcgg cgatgccgac atcgaaacag cgctcgggga acgcttgcgc gaagcggtcg
     1261 agtccggtgc ccgacggcat tgccgcggtg atggcgacga tcttggggtc gacgcgggct
     1321 tcctcgatca ggctttgcgc gaacaccttg gtgtagctcg gcggcgtccc ggcgacttgg
     1381 gtttcttgcg cgccggtgac gacattgaac ttgccgacgc catgaaactt gtcggcggac
     1441 acttcggcgg gcgcatagcc cttgcccttc ttcgtgacga catggaccag gaccgggccg
     1501 ttcttcatgg cgcgcacatt gcgcagcacg ggaagaaggt gatcgaaatt gtgtccgtcg
     1561 atcgggccga catagtagaa accgagttcc tcgaacaagg tgccgccggt ccagaagccg
     1621 cgggtgaact cttcggcccg cgccgcgcgc cgctcccagc tcttgggcaa aatcttgtgg
     1681 gcgagctgtt tcaggaagcg gcggaaacgc cgataggtgc cccccgagat gagcttggcg
     1741 agataagcgc tcatggcgcc gacgggcggc gcaatcgaca tatcgttgtc gttgaggatc
     1801 acgatcagcc gcgaatccaa cgcgccggca ttgttcatgg cctcgtaagc catgccggcg
     1861 ctcatcgccc cgtccccgat gacggcgatg acgttgtggc tcttgccgtc gaggtcgcgg
     1921 gccacggcca tgccgacgcc ggccgagatc gaggttgagg agtgaccggc gccgaacgtg
     1981 tcgtattcgc tctcggtccg cttgcagaag cccgagagac cgccggcttg acggagcgtg
     2041 cgaatgcggc tgcggcggcc ggtgagaatc ttgtgcggat aggactggtg tccgacgtcc
     2101 cagatcaacc ggtcgcgcgg ggtgtcgaag acgtggtgaa gcgcgatggt gagctcgacc
     2161 acgccgaggc ccgcgccgag atggccgccg gtcaccgaaa ccgccgcgat cagctcctcc
     2221 cgcaactcct ccgccagttg tttcagctcc gcgtccgcaa gcgcgcgcaa atccgcaggc
     2281 tcgtggatgc gatcgagcag cggcgtggga ctatcggcac tcattcgctt tacccggatg
     2341 atttctggga aaggctggag atgatcaagc cgtttattcc tttatcggcc cgcccttccg
     2401 cttgcaacta tcctatgagc cgggccaaat cgtgtcacct tgcgcgttgc cgcaggccca
     2461 agcgaacctg gctcaagggg ggtcaggtct cgacgtcgag gggctccgtg ccggtgggct
     2521 gcccgttctg attgagagag attttctcga cgcgcgcctc ggccttgcgc agaagcgcat
     2581 cgcaatgggt cttgagcgct tcgccccgct catagatgag gatcgaggct tcaagctcga
     2641 catcgccctt ttccaggcga tcgacaatgg tctcgagctc cttcaaggcc tgctcgaagc
     2701 tcatgctctt gatgtctgag tgatctgtcg gctcggccat ggtcctggtt agcctcttcc
     2761 cgcgttccca atcaagggct gaactgggtt ccggcggcgg cgcccatcaa ggtctggacg
     2821 tgaatggcgg tcgagctcgc cagcgcctcg agatcgtagc cgccttcaag cgtcgagacg
     2881 agcttgccgc cgcaatgctt ttcggccgcc tccaacagtt tctcggtgat ccaagcgaaa
     2941 tccgattcga gcaacctgag attcgcgagc ggatccttga gatgcgcgtc gaagccggcc
     3001 gagacgagaa ggaaatcggg agcgaaggct tcgagcgccg gaaagattcg tctctcgtag
     3061 gcgtctcgaa aatcgtcgcc atcgtcgcca ggcttgagcg gcgcgttgaa gatattgccc
     3121 acgccggtct catcgcgtgc gccggtgccg ggaaagagcg gcatctggtg ggtcgagccg
     3181 tagaacaggt cgcggtcgga ccagaacgcg gcctgggtgc cgttgccgtg atggacgtcg
     3241 aaatcgatca ccgccacgcg ctcggcaccg tgggcagcgc gggcgtggag cgcagcaatg
     3301 gcgacgctat tgaacatgca gaatcccatc gcccgcgaag gctcggcgtg gtgtcccgga
     3361 ggccgcaccg cgcagaaggc gttgtcggcc ttgcccgcca tgacttgatc gacggcatag
     3421 aggctggcgc cagtggccct gagcgctgcg tcccagcttc ccggcgagac gaccgtgtcc
     3481 atatcgagaa atttatgatc ccgcgccggg atggaggcgc ggatcatctc cacataggcc
     3541 ttgggatgaa gccgctcgat cgcggcgagg tcggcgagcg gcgcctcctc gcgcttcagc
     3601 gtcttgaagg caggtgcggc aagagcgtca tcgatggcgc ggagccggtc ggcccgttcg
     3661 ggatgcccgt agccggtgtc gtgcagaagg caggcggggt gggtcaaaag aagcgtggtc
     3721 atcgttggtg agtcgtctag agactcagtc gcctgcttct tcgggctcaa gaagatcggc
     3781 aatgacggcg ggcagctttt ccacgagctg ttccaacgtc tcggcctcgg cgttgaggcc
     3841 gaacaggtcg ctctgctcca caaaccagac gttggcttcg ggatcgtgtg ccgctttgac
     3901 gaggatgctg cgaaccatga caaatttgac ctagagtgaa accgagcgtc atcatatacg
     3961 cgcacccttg gcccaccaag tcactcgagt agcctcagcg atgcccatcg ttccggccac
     4021 gccagatgcc atcaggcgcg cggcccaggc gctcgcccgc ggcgagatcg tggccttccc
     4081 caccgagacc gtttacgggc ttggcgccaa cgcgctcgac gcgcgtgccg tcgccaaggt
     4141 gttcgcggcg aaggagcgcc cgcgcttcaa cccgctgatc gtgcatgtgt cccatctcgc
     4201 cgaggccggc gaatacggtc tggcaaacgg agcgacacgg acgctcgccg aggccttctg
     4261 gccgggtcct ttgacgctcg tcctcaagcg gcggtcaact tgcgcagttg ccgacctcgt
     4321 cagcgcgggc ctcgacacga tcgcactccg cgccccggcc caccccgttg cgcaggcact
     4381 cctcgccaaa gccgccctgc cgatcgcagc accaagcgcg aaccggtcgg ggcgtctcag
     4441 cccaaccgcg gcggcgcatg tcgaggccga gctcggcgat ctccccgtca tgatcctcga
     4501 cgggggtccg tctcctctcg gcatcgagtc gacggtggtg agcgtggcgg ggaaggagcc
     4561 tgccctgttg cggccgggag cgatcgcgcg cgatgcgatc gagagcgtgc tgggggcgcg
     4621 tctcgcagcg gcgagcgggc cagagcggaa gacgtcaccg gggcagcagc ctcgccacta
     4681 tgcgcctcac acgccgctcc ggctcgatgc ggtcacggtt cgccccgacg aagccctgct
     4741 cgctttcggt gacaatgtgc cagcgcacgc acgcgcgacg atcaatctca gcccgagcgg
     4801 caatctcgaa gaggccgccg ccaaactatt tgccgcgctg cgcgagctcg accaggcggg
     4861 cgtgaagacc atcgccgtga tgcccattcc cgccacgggg cttggcgaag ccatcaacga
     4921 ccgcttgcgg cgcgcggccg atcggggcta gcgctgatcc cgatgaaacc cgcgccgaag
     4981 catactccgc caagccccga cacgcgcgat gcactcgtgc ggatcgtcgg cgccgagcac
     5041 gccatccgcg acgaggcgga catgggtccc tatctcgtgg aatggcgcga ccgctaccac
     5101 ggcaaggcgg cgctcgtgct caagccggca gacactggcg aggtttcctc gatccttaaa
     5161 ctcgccaacg agaccaggac cgcgatcgtg ccgcaaggcg gcaataccgg cctcgtcggc
     5221 ggccagatcc cgttcgagag cggacacgaa gtggtgcttt cgctcgagcg gctcaatcgc
     5281 gtgcgcgaca tcgacctcgc ctcgaacacc atgacggttg aggcagggct tccgctggcc
     5341 caggcgcaac atgtcgcggc gagcgcgggc cggctgtttc cgttgagcct tgcgtcggag
     5401 ggaagcgcgc agatcggggg cgtgctcgcg accaatgccg gcggcatggc cgtgcttgcc
     5461 tatggcagcg cgcgggagct tgcgcttggg ctcgaagtcg tgctggcgga cggagaggtg
     5521 tggaacgggc tcaagtcgtt gcgcaaggac aattcgggct atgacctgaa ggacctgatc
     5581 atcggctccg aaggcacgct cggcatcatc acggccgccg tcctgcgcct cttccccaag
     5641 attgcagaga aggtcacctg catggcgggc ttgaatgcac tcgaaacggc gccggccttt
     5701 tttgcccgcg cctttgcgcg ggccggcccc gctttgacgg cgttcgagct cctgccgcgc
     5761 atcgggatcg agttcgtgct ccgccatggc agcgggtcgc gcgatccctt tccctcttcc
     5821 tatcaatggt acgcgctgtt cgaactcaca agcccgcgcg aaggcaacga gcttgctccg
     5881 ctggccgaat cacttcttgc ccaggggctc gaggcgggcg agatcgccga cgctgtcgtt
     5941 gcaacatcgc tatcgcaagg cgaggcgctg tggcgcctgc gcgagctgat gagcgaagtg
     6001 caaaaggaag agggcggcag catcaagcac gatatcgccg tgccggtggc gcgggtgccg
     6061 gagttcgtcg cccgcgccaa tcggctggtc gagctgatga tccccggcgc gcgcccggtc
     6121 ccgttcgggc atttcggcga cggcaacatt cactacaatg tcagccagcc gcccggcatg
     6181 gagcggacgg tgttcctagc gaattgggag gcgctcaacg ccgccgtgca cgagatcgtg
     6241 ctcgatctcg gcggctcgat cagcgcggag cacgggatcg gacgcttgaa gcgcgatctc
     6301 cttccccatg ccaaagaccc gctggagctt gcgctgatgc ggaagatcaa ggccgcgttc
     6361 gaccccaacc tcattctcaa tcccggaaag ctgctgcgat gaagcgcctt gagaccgatg
     6421 cgctgtggac ggaaatcgac gagtatctca cccgctccct cgttgcgagc gacccggttc
     6481 ttgacgcggc tctgcaagca accgaagccg cggcgcttcc tgcgatcaac gtcaccccca
     6541 gccagggcaa gctcctcatg ctgcttgcgc gcgcgatcgc ggcccggcgc atcctggaga
     6601 tcggcacgct tggcggttac agcacgatct ggctcgcgcg cggccttgtc cccgatggac
     6661 gcttgatcac catcgagtcg aaccccgact gcgccaaagt cgcccgcgcc aatctcgccc
     6721 atgccgggct cagcggccaa gtcgatgtgc gtgtgggccg cgcccaggac gtgttgccgg
     6781 agctcgcggc ggaagctccg ttcgatctcg tcttcatcga cgccgacaag cccggcacgc
     6841 ccggctattt ccaatgggcg gtcaagctcg cgcggcgggg ttcgcttatc gtcgtcgaca
     6901 atgtcgtgcg cgcgggcgcc attctcgcag ctgagggcga cgatcatgtg cagggcatgc
     6961 gccgcttctt cgaacttgcc gcctccgacc cgcgcgtcac cgcaaccgcg gtccagaccg
     7021 tgggggccaa gggccacgac ggcttcgcga tcctcctcgt caccaccgcg ccgtagcgca
     7081 ggagtgtcag cgctccggcg ccttggcttc ctcaagcagc gccgcttcag ctttcttcag
     7141 ctcgcccaac tgcgcctcgt cgaatttcgg gaattgcaga tcgagggcct ccatcctttc
     7201 gaccagggtg gcggcgacca cgagacgggc gaaccatttg cgatcggccg ggacgacata
     7261 ccagggcgcg tgtttggtgg ccgtatgacg gatcgtatcg tcataggcct tgataaagtt
     7321 gtcccacagc ttgcgctcct cgacgtcatg gaaggaaaac ttccatctct tttcgggatc
     7381 ggcgatgcgt gcgagaaggc gcttcgactg ctcccccttg gaaatgtgga ggaagaattt
     7441 gagcggcacg gtgccgctgc gggcgaggtg cagctcgaaa ttgttgatgt cctcgtagcg
     7501 ctccttccag atcgtgtcgg tgacaagcct gttcggaagg ttttgtttgt cgagcagctc
     7561 gggatgtacg cgcacgacga tggtttcttc ataataggac cgattgtaaa cgccgacgtg
     7621 gccgcgcgcc ggaagcgcca tcgtggttcg ccacaaaaag tcgtgagcaa gctcgagcgc
     7681 gctcggctgc ttgaacgaat gcacctcgca gccttgcggg ttgatccccg ataggacgtg
     7741 cttgatgacg ccgtctttgc ccgatgtgtc cggtccttgc aggatggcga gaacggccca
     7801 gcgcccctcc gcatagagcc gctcttgcaa cttggagagc cgcttggtcc cggccgcaag
     7861 cagcgcgtcg gcctcctcct tttcgatggc aaggccgaaa gtgtcactcg gatcgcgatc
     7921 ggcgagcttg aagtcgccgc ctttgtcgat gcggcaatgc gcgatgattt cggatggaag
     7981 catcggtctc cccggctcta gagcagcgat ggcgcgagcg gctcctgaat gccgggctcc
     8041 tcgcgtttcg aatcgttgat cttgggatgc aactcgatcg cctcgaagag gtcgtcgggg
     8101 gcgggctgca gcaattcccg cgcagcctcc ggcgccgttc cctccacgtc aagccaggcg
     8161 tcgaaatctc ggtcagcaag aacgacgggc atgcggtcgt gtaacggcgc gaccgtgctg
     8221 ttggccggac aggtcaggat ggcgacggtg tccatctcgt cgcccttgct gtcgcgccag
     8281 cgctcataga tgccggcaaa ggcgatcagc cgggcgtcgt gcgggcgaag gaggaaaggc
     8341 cgacgcttcc ccttaggccc cgtccattcg tagaacccgt cggcgggcac gaggcagcgc
     8401 cggtaacgca tggcgtggcg gaacgatgcc ttgtccagcg cctcctcggc gcgggcattg
     8461 atgagcgtcg ggaaccgctt cacgtccttg acgaagaacg gcaacaggcc ccagcgcatg
     8521 agcctgaagc ggcgaagccc ctctctgtcg cggtggacaa cggcgattgg ctgggttggg
     8581 gcgatattga agcgcgccgg aaagttcggc gtatcgcgat aaccaaaata ggcgcgcacg
     8641 gcctctggcg gggaggtcag gctgtagcgg gagcacatga cgggcgaaat cctgtcgcgt
     8701 tcgagcgatc atgatcggcg aacgaaccgc ctattccaag tcttggtttc gctgccgagg
     8761 ccttgttcga gaggtgcacg cctccctaca tacatggtgc atttcgggca cgattcactc
     8821 catgaacgag cacatcccga ccgacaccgg cctcgcgcat tgcgatggcg cgagcatggt
     8881 cgtgctcaat caaaacaagg tgttgctggt cagacgcggg cgcgccccct tcgccggact
     8941 atggagcctg cccggcggca agatcgagcg gggcgagacg ccgcgcgagg ccgcccgccg
     9001 cgagctcaag gaggagaccg gcgtcgaggc ggatgtggag ggtgtgctcg acagggtcac
     9061 cgtttcggct cccggcgacg cgacctaccg gctgaccgtg ttttacggac ggcccgtcgc
     9121 cggcgccctc aaggccggcg gcgacgcctg gacggcggaa tgggttcatc tcgacgaggt
     9181 cgatgcgcgc cccatgaccc aggggactgc cgagctgatt tggacggccg cccaccggct
     9241 tcggcgctcg tgatggctag atttcaacaa aaatcggcgt tactcagtct cccatgggac
     9301 ggggcaaaag gcttcgctta aggctcgttg ccggcctgct tggcgcggcc tgggcctcca
     9361 tggtcctggc accggcagcc gaggcgcagt tcttctggtg gttcgatcag ccgaagacgg
     9421 cccccaagcc tcgtgccaag gccaggcctc cgggcgacga cctgcccgcc atcgtaccgc
     9481 gcgaaagcac cgcgcccccg ccgcccgacg atcgcgccta tgacgacaag ctgatgcggc
     9541 tcgccgagat cctgggcgcc atccactatt tgcgcgagct gtgcggggcc gaggaagggc
     9601 agacctggcg cgagcagatg aaagagatct tgcggaacga gggaacgacc gccgtccgcc
     9661 gcgcgaagct ggtcaacgcc ttcaacgacg gttatcgcgg ctatcggcgc agttatcgca
     9721 cctgcaccca gtccgccacg cttgccacaa accgcttcag caccgaaggg gcaagcatcg
     9781 cagcggcgct cgcgcagagc tcgacctttg ccagcaaggg cccgcccgac gctgagcccc
     9841 aagcctcgcc ggggaccaat caggacgcct ctgcccccgc ctcgggcgag gcgggggccg
     9901 gggcccagcc cgcgcaaggg gccggcgaga cgggcgcaag cgcggcgcct gtggcgcccg
     9961 gcggaccgcc tggcccgcaa gagccggaac cacccgacta acccggttcc gcgacgagcc
    10021 gcctcgaccg ctggcatggt gccgccgccc aggccggcga tgccggaaag ccctgccgtc
    10081 agaaacgcag cggcactcag tggccagaca agtgcgtcca cccggcgaaa acgcttcggc
    10141 ctaaagtacc agcaccgtcg ccaatcccag catgagtccc atgccggcga tatcggttcc
    10201 cgtggtgagg aagatgctcg atgcggtgca gggatcggcg ccgaatcgtc gcagcgtcaa
    10261 tggcaccagc acaccgaaca agcctgaagc catgcaggca ccggtcatcg cgaacagaat
    10321 aacaaggccg aggatcagcc cctgatcgtt gccgcttcca ccggcgctcc accacatcgc
    10381 cacgcccgcg acgatacccg tcagcaaacc atttattgcg ccaagggtca gctccttcgc
    10441 gatcaacttg cgcacgggat ggctgttcaa gtccccaagc gtcattccgc gaagcgtgat
    10501 cgctagcgcc tggcatcccg tgttgccgct ttggcccgca aggatcggca ggaaagctgc
    10561 cagtgccacg atctgagtaa tggtgccttc gaaaaggcta accacgaagc ccgctgcgaa
    10621 agcagtcaac aggttcacct gcagccaggg atgacgcatc ttgaaagccg tccagatccc
    10681 ggtgtagaga cgttcctcct tatccacacc gaccatctgg cccgactgcg cgctgatctc
    10741 gatcgcctga cgctcgaaaa ggcgccagcc gcgcaccacg ccgatcacgt gtcggtcggc
    10801 gtccaccacg ggatacactg ggtagtgccg atgtatagcc gccttcacgg cgtcggcgag
    10861 gtccttgtcg atcgtcagcg caaaaggctc gggcaacatg acgtccgcca acgtttgatt
    10921 cggcttcgcc aggatcaagt cgcgaatcac ggtcagaccg acgagcttgt cgtccgcgtc
    10981 ggtgacgtac agataggtga tctgctgagg ggtctcgtga tttcgcagaa aggcgatcgc
    11041 ttcctccacg gtcgtcgtct gcggaagcac ggccagcgcc gggtccatta tttcgcctac
    11101 ggtgttctct acgacctccg ccagagactc cgaggcctgc aactgcaatt ccggaggcag
    11161 ataggctttg atacggtcga cgagtatctg cggcagctcg gtaagaaccg ctccgatacg
    11221 ctcgggcgtt tccttgacca gcatctgcgc cgcatccatg ggcacgcgcc cacgaaccgc
    11281 tcgaaccaag cctgcaatgt cttcttgctc gctcatcgaa tgctctccga acttcccgcc
    11341 gcaatgcgag cggagctccc taccccggct cttcggcggg cgggcgggcg aaggggttgc
    11401 tagtattgga agagcgcccg gcctgtcaaa aggccagcac ggaaccgggc gggcggtcca
    11461 gccggaacca ccggactaac cctaaagata accgttagtt cactctagtg ctcccggcgg
    11521 cggaggggcg tataaattgt gacgtctcac gatgtccgcc agccacaagg gggcgctaga
    11581 gttgcggtcg gtacggcaag tgccgcgatg ctcggaaagc gctgcgcata agcaggcggc
    11641 gctccgatac atcctcgatg cttgggaaga ggcgctgcac gacggcatcg agcccgaaat
    11701 gctcgccaac gccgctctgt ttgccgcgct taccgatctc atcgacgtct atggggagga
    11761 cgcggtcgca atgatgacga acgggctgtc gcgccgcatc ctccacggcg agttcacgct
    11821 cacgcacccg ccgctcgctt gagaggcggc ggcgtcattc tagcgtaacc cgcctttcgt
    11881 cattccggcg caagccggaa ttcagggcct ctagctcaga gcttgttgca cctggccccc
    11941 ggctttcgcc gcggtgacga tgaggcctat ccccgcacca aaccgtcaag caggagcagg
    12001 agcccgaagg cgagcagcac cgcgccggcg attcgattga tcagcttcag gcggccttcg
    12061 gtgagcttgt gtctgatcgt ggcgatgagt tccgagagac cgagccacca gagaagactg
    12121 ccgcccatca cggcggcgac aagcaacagg gcttcgagat aggtgttgag accgccgatg
    12181 agtgaaccga ggccgccgat catcgcggcc atgccaagca cggcgccggg attggtgacg
    12241 gtgagaaaga aggtctgcgg gatgattccc atgtgctcca agaggtgcga cttctggccg
    12301 accgggatgg tcatggcggg cttggcgagc agaagcgcga ggccaaaacc gatcaggagc
    12361 agaccgccga cgaactggat ttgccccgaa taggtgctca tgacattgga aattgccgtg
    12421 acgctgaagg cggcgatggc ggccaaaaga ccgtcgccga gcaccgcgcc gataccggcc
    12481 gcgagcccgg cccagaagcc gcctctgacg gcgcgctgaa tcacgaggac attcaccggg
    12541 ccgatgggcg cggccaccag cacgccgatg ccgatcccgg caagcacgag ctgaagggtg
    12601 gttaggacga tttgcgcgcc cgactgcaag gccaggccct attcgctcat cgaggatggc
    12661 accgggcagg cgcgtggcac ggtgaccgac ccgagttgga cttgcgctcg tccgccgatc
    12721 tgcgagattg atcgcgcact catgggttct gcatcaatcg ccggagacgc ttcgtcatga
    12781 tggccctcgc cgcattggta gcgggcgctg ccgccgttgt ctacgtgcac aacgaagcgt
    12841 caaacgcgct tgccgcacag ggcgccgcga ctgtcatggc tgccggcacg gcagatcaag
    12901 cccctaaggc actgtacgat ttcaacgcgc tacccgatcc ggtcaagcgc atgatcgagg
    12961 cgatcgcgga agccgcgcaa agcggtgaga tcgagaagat gcggccagtc ctcgaatcga
    13021 acgagctcaa gccgatgatc gccgccgatc atgtcgacga tcccatcgcc ttctggaaaa
    13081 aggaatccgc cgacggctcc ggccgcgacg ttctcgccgc catgctcaac gtgatgtccg
    13141 cgggctacgt ccgcgtcggc aaaggacagg atgaaatgta tgtgtggccc tatttcgccg
    13201 agacggggct ttccgcgctc acgcccgccc aagaggtcga gctttaccgc atcgtgccgc
    13261 cgcaactcgc ggccccgatg aagaaatccg gcaaatacag ctactacaag ctcggcatcg
    13321 cgccgaccgg cgtctggcac tatttccttc ggtaacccgc gcccgccggc ggcaaagcct
    13381 cgctgaaccg gaccggctcg cccttcgcct cgccaatctc gccctcccac atggcaagcc
    13441 gcccgcgcac gagcgtgccc ttcggccagc cccgcagcgt acgcccggcg aacggggtcc
    13501 agccgcattt gctgccgatc catgcatcct cgaccacgcg ctcctgcttg aggtcgacga
    13561 tggtgaggtc ggcgtcgtag cccacggcga tgcgcccctt gccggcgatg ccgaagacgc
    13621 gctgcgggcc tgcgctggtc agatcgacga aacgggcaag gctcaggcgg ccggcattga
    13681 catggtcgag catgaccgga acgagcgtct gcacgccggg cattcccgac ggcgtcgcgg
    13741 ggtagctctt ggctttctcg gcgagcgtgt gaggcgcgtg gtcggagccg agcacatcga
    13801 cgacgccgcg ttcgatgccc caccacagcc tgtcctggtg gtcgcgatcg cggatcggcg
    13861 ggttcatttg cgctttcgtg cccagtcgct cgtaggcgtc cggtgctgca agggtgaggt
    13921 gttgcggcgt gacctcgacc gaggcgaagt ccttgtgaag cgcgagcagc tccacctcct
    13981 cggccgtcga gacatggagc acgtgcaccc gctttccggc gtgagctgcg agccgcaaca
    14041 gacgctctgt cgaggcgagc gcggcctcag cgtcgcgcca ttcgtaatgg ctcgaagggt
    14101 cgccctcgcg ctgtagcgca atgcgctctc tcagccttgc ctcattttcg gcgtggaagg
    14161 aggcccgacg gttgatcgtg gcgagaatgc gcgcgagcgt cgcctcatcg tcgacgagga
    14221 gatcgccggt cgatgagccg agaaagacct tgaccccggc cgcaccggga aggcgctcga
    14281 gttcggcgag gtcgccgaca ttgtcttgcg tcgctccaac gaagaaggcg aagtcgcaat
    14341 gcatccgccc cgaagcggct ctcacctttg ccgtcaaggc atcggcgctc gtcgtcggcg
    14401 ggcgcgtgtt cggcatctcg aatacggcgg tgacgccgcc gagcaccgcc gaccggctgc
    14461 ccgaggcgag atcctccttg tgctcgaggc cgggctcgcg gaaatgaacc tggctatcga
    14521 tgacgcccgg caggatatgg agcccggtcg cgtcgatggt ccgggcggcc atgccctcgc
    14581 cgattgcgcc agtttcggcg atgcggccat ctctcacgcc aatatcggcg tgcccctcgc
    14641 ctgcatgatt gacgacagtt ccgcctttga aaacaagatc gaaggtttcg gccaagggcg
    14701 tctcttgcgg gggccagccc cctcccataa atgtcggtgg ccttggttag cagatggacc
    14761 ttgtatgagc cattgtcatg cgagcgtgtt gccctcgcgc gccgtcttga ggatcgccgg
    14821 cgccgatgcc ggcaatttcc tgcaaggcct catcaccaac gatatcggca aggcacaggg
    14881 ggcgaacggc gtccacgccg gcctgctctc ccctcaaggc aaggtattgt tcgatttctt
    14941 cctcgtcgcc gacgggggcg gctttctcgt cgatgtcgcg aaagagaagg ccgaggacct
    15001 tgcgaaacgg ctcgccttct accggctccg ggcaacggtc gagattggtg aagatccttc
    15061 gcgggaggtc gccgcctgtt ggggcgggaa gcctgtgcaa ccgaccggcg gcatcgtctt
    15121 tgccgatccg aggctgcctg cgctaggcta ccgcgctgtc ctggcgaagg gcacaagccc
    15181 tacggcactc ggttgtgacg ccgcgaccga gcaggactac cacgattttc gcatcggcct
    15241 cggcgtgccg gaaggcggcc gcgactatgc cttcggcgat gtcttcccgc atgaggcgct
    15301 gttcgaccag ctgagcggtg tcgatttcaa caaaggctgc ttcgtcggcc aggaggtcgt
    15361 ctcgcgcatg gagcatcgaa gcacggcgcg aaaacgtatc ctgccggtcg aaggcgctgc
    15421 agcgctgccg gctccgggca cgagcatcga ggcggccggc gtggccatcg ggactctcgg
    15481 ctcggtgagc gggtcggcgg gcctcgcgct cattcggctc gaccgcgtcg aggaagccct
    15541 ggcgcggggc gagacgcttg cggccggtga ggtgaagatc gcgctccggc gccccgcctt
    15601 cgcccgcttc gcggtgcctc cgatggaagt cccggcatga gtgcgaaacc cacgcgctgt
    15661 ccgtggcccg ggaccgatcc cctttatatc gcctaccacg tcgaggaatg gggcgtgccc
    15721 gaacgcgacg accgcgcgct ctacgagaag ctcgtgctcg acgggtttca ggcgggactc
    15781 tcctggatca cgatcttgcg caaacgtgag gcgttccggc gggcgttcga cggtttcgat
    15841 cccgaacggg tcgcgcgcta ctcgatgcgg cgcgtcgagg ccctgatgcg ggacgagggt
    15901 atcgtgcgca atcgcatgaa gatcgagggc gccatcgcct cggcgcgcgc ctggctcaag
    15961 atcatggaga cgagcgcggg gttcgcaccc tttctatggg atttcgttgg cggcaagccg
    16021 ctcgtcaatc gctacaggcg catcagcgag atccctgccg agacggagct gtcgcgcacg
    16081 atctcgaagg aactcaaagc gcggggcttc aagttctgcg gcccgacgat catctatgcc
    16141 ttcatgcagg cgaccgggat ggtcaacgac catttgaccg gctgcccgcg ccacgccgct
    16201 tgcgcgcggc tcggcgcgac gccacctaac ctttcgtgac gcgtggatcg cggcccctgg
    16261 gataggtgcg ctcttcctgg actttgccgt tccgcttctt gattttgacc gaaccttctt
    16321 tgccgacggc ccgctccaag accccgcctt tcgttgcccc cgccttgctg gcgaaggatt
    16381 tgaccgtctg gccggtgccg tcgttcttca gcacccagcg tttcgtcttg gcgtcatgcg
    16441 acagtgtata cttggccagt tttgccatat gcatctccca gtgacagatc aggtccgcct
    16501 gcactctgac aattgccgtc cgacgaatgc ccgcattggc gaggttcgtc cgtttcttcg
    16561 tggctgctat cgcgaaaata gtcaatgcct tggcaagccg ctccagcacc ggccgagacg
    16621 atgagaggga ccaggatcgt tcagaacgcc gccgccagca cgagtgcgcg cgcctggcag
    16681 cgcatgttga gcgggcgccg gcttgacctg ctcgacccct cgccgctcga catcgagatc
    16741 gaggacatcg cgcacgggct cgcccgcgtc gcgcgctgga acggccagac gagcggcgcg
    16801 cacgcctttt ccgttgcgga gcacagtctc ctggtcgagc tcgtcgccgg cgagctcgag
    16861 cccaagctcg gccgcgactt caggctcgca gcgctgatcc acgacgcgcc cgaatatgtc
    16921 atcggcgact tgatcagccc cttcaaggcg gcgctgtcac tcgattatcg cgcgttcgag
    16981 gcaaaactgc tcgccgccat ccatgttcgt ttcggcttac cctcggagct tcccgccgat
    17041 gtcgtcaaat cgatcaaacg cgccgataag atcgcagcct attacgaggc aaccggactt
    17101 gcgggattct cgcgcgccga ggcgcggcga ttcttcggcc agccgcgggg cttgagcgct
    17161 cggctcgttg cacgtctttc gcagttagac ccgttgcctg cgtcccgcgg cgaggccgcg
    17221 tttctaaaac ggttcaatga gttgcatcga tgatgcgatc gccgcagaga ctgcgcgcga
    17281 gaaggcagac gagagaatga acaccctgat tttggccaca gaagcggcta gagtgccgcc
    17341 gaccatgagt cccgaaacca tctatgtcgc gcccttgagc ctcgtcgagg ccacggtcgc
    17401 agacgccaag gtcagccatt tggtgacgct gatcaacggc gatacagcca ttccgacgcc
    17461 atccggaatt ggccccgacc ggcatctcag gctcgccatg aacgatatct gcgaaccgca
    17521 gcccggcctc gtgcttccct gcgtcagcca cgtcggcgat ctcataaggt tcacgcggga
    17581 gtgggaccgg caggcgccgc tcctcatcca ttgctgggcc gggatcagcc gttcgaccgc
    17641 agccgcctac atcgcgcttt gcgcgctcaa tcgggaaggc accgagcatg agctcgcctg
    17701 ggcgcttcgc cgcgcctcgc ccacagccta ccccaatcgc ctcttggtgg cgctcgccga
    17761 tgaggccttg gcgcgggacg ggcgcatgat cgccgcggtc gaggacatcg gccgcgggca
    17821 gatcgcggag gaagccgacg tgttctcatt gcgtgcgctc cttgccgcct gagacatggc
    17881 cgagccgaga cgcaagccac cacccccgca ggaagcgcgc gggggcgatc cgcagatcga
    17941 gctcgggctc aacgccgcca tcgtcacggt ccgagagcgc caaccgcata ttctcgtggt
    18001 gcgttccggc acggcgggat ccggcgcgtg ggacgcgctt cccttcggcc cgttcgcgcc
    18061 ccgcgcccat cgcacgctcg agatcggctt gcgcgaatgg gtcaagcgtc agaccggact
    18121 cgatcttggc tatgtggagc aactctatac gttcggggat cgcgggcgcc atgccgagcc
    18181 cggcggggcg catacgcttt cggtcggcta tctggcgttg acccgcggcg cgggcgagga
    18241 gctgctcggc tcgcactggt cgccctggta cgcctatttc ccctgggagg attggcgtca
    18301 ggggagaccc gacattctca ccgatgagat cgagccgcgg ctcaaagatt gggccgagcg
    18361 gccgccgctt cccgacgagc ccgcgcgccc gctcagacgg caagagcggc tcaggatcag
    18421 ctttgggatc ggcggaggct gggacgagga gacggtgctc gagcgctacg agcttcttta
    18481 cgaggccggt ctcgtggccg aggcgcggcg cgacggccgc cccgccgcgt cgcaatggcc
    18541 ggccctgccc gagcttggac gcccgatgga gttcgaccat cgccgcatcc ttgccaccgc
    18601 gctcggacgg cttcgcggca agctcaaata ccgcccggta gtgttcgagc tcctgccgcc
    18661 ggagttcacg ctttacgaac tgcaaaagac agtcgaagcc atctccggca cgcttctgca
    18721 caagcagaat ttcagacgtc tggtcgagca aggcggcctt gtcgaggcga ccggaaacgt
    18781 ttcgaccgcg accggagggc gtcccgcgcg gctctaccgc ttccgccgcg aagtcgtgct
    18841 cgaacgtcca gccccgggcc tgcgcgtcaa ggccggacgt ggataaaggg aacgtctcgc
    18901 ggtgaccggc ggtttttttg gtgaagttca gctataggag acgagtaggt gaccccgaat
    18961 gaaacgtatc ggaagcgtaa cgcctctttg cgcacggtcc tggttgtcct ctcccttctt
    19021 ttggttgggg cgctgattgc ggtgtcgcaa caaggagcgt caaaagagcg ccaaatcgac
    19081 agcaagcagg ggccgatcaa ggtcgaaagg gtggccaaag gactcaagca accctggggt
    19141 cttgcctttc tccccgacgg gcggatgctg gtgacggaaa ggcccgggcg cttgcggatc
    19201 gtgacgaagg acggcgcgct ttctgaaccc ctgaaaggcg tgcccgaggt ggttgcccgg
    19261 ggccaaggtg gacttctgga cgtcgcgctc gatcccgctt tcgccgagaa cggccttgtc
    19321 tatttgtcct attcggagcc tggcgaaggc ggcgccggaa cggcggtcgc gcgcggaaag
    19381 ttgggcgacg gcagcctcga cgatgtcgag gtcgtcttca ggcagaagcc caaggtcgat
    19441 ggcggcaacc atttcggctc acggcttgcc ttcagccctg acggcaagct gttcgtcacg
    19501 ctcggcgatc gcttcaaatt cgatccggcg caggatcttg cgagccatat cggcaagatc
    19561 gtgcgcatca atcccgacgg ctccgtgccc gacgacaatc ccttcgtcgg ccaggagggc
    19621 aagctgcccg agatctggtc ctacggtcac cgcaatgcgc aaggcgccgc cattcacccg
    19681 gaaaccggga agctttggga gtccgagttc ggcccgcttg gaggcgacga actcaacatc
    19741 ccggaagcgg gcaagaatta cggctggccg gtggtgagct ggggaaagca ctatgacggc
    19801 accgacattc ccgatccgac cgaacgtccg gaattcgccg acgccatcta tcattggaac
    19861 cccgtgattt cgccttcagg catcgccttc tacacgggcg acgccattcc ggcctggaag
    19921 ggcaatctcc tcatcgccgg tctctcttcg gaagcgatcg tccggcttga gctcgacggc
    19981 gagaaagtcg caggggaaga gcggatcgcc atgggcacgc gcattcgcga cgtggcgcaa
    20041 ggtcccgacg gcgccgtcta tgcgctgacc gacgagggcg gcgagatctt gaagctcacg
    20101 ccggcccggt gagcgtcctc gcgacgggaa cggccggcaa gatctttttc atggcacaac
    20161 gcgggctctc acttggattc ttcggcgtat tcggccgatc ggccgagttg cgcgaacttg
    20221 acaaggctct gcgctcggtc gacctgcatc ccaatctcgt gcctgaagcg gtcaagctca
    20281 ccgctgtcag cctgctgaag gaggctgatc gcgagccgga acctgccgca taccgcgccg
    20341 ccgccgagat catcgcctat tgcatggtcg gcagcgatgc gttcgcaagc gcaaatgacg
    20401 caaatcttgc cgccaaagtc gaacggcgaa tcgaggcggc gctcgagccc ggcacgacgc
    20461 tggatgccaa gcttgtgctc ctgaccttgc acgcgcgggt catccagcca agcgtcgtcg
    20521 aggattttgg gctcgaaagc ggcggttgaa tctcttggcg cgtgccgcag agttgccata
    20581 ctgaagccgt cgatagggcc ctgattttcg aggcctcctt caccctcttt cgattcgtca
    20641 aatgtccgcg atcatctcca tcaagaacgt gtcgaagacc tacgcgacgg gcttcgaggc
    20701 gctcaagagc atcaacctcg aaataaaaaa gggcgagatc ttcgccctgc tcggaccgaa
    20761 tggcgccggg aaaaccacgc tgatcaacat catctgcggg atcgtcacgc ctagtgccgg
    20821 caaagtgacc atcgacggtc atgatatcgt gaccgattac cgtgccgcgc gatcgctgat
    20881 cggtctcgtg ccgcaggagc ttaccgccga catgttcgaa acggtgtgga cgaccgtctc
    20941 cttcagccgc gggctgtgga gcaaatggga gaatcccggt catatcgaga gcgtgctgag
    21001 gagcctctcc ctgtgggatc ggaaggactc gaaactcatg acgctgtcag gcggcatgaa
    21061 gcgccgcgtc ttgatcgcca aggccctgtc gcatgaaccc accatcctct ttctcgacga
    21121 accgaccgcc ggcgtcgatg tcgagctgcg caagggcatg tgggacaatg tccggtcgct
    21181 gcgcgactcc ggcgtgaccg tcatcctcac cacccactac atccacgagg ccgaggacat
    21241 ggcggaccgg atcggcgtga tcaacaaggg cgagctgatc ctggtcgagg acaaggccga
    21301 gctgatgcac aagctcggca agaagcagct cacgcttcaa ctgcatcagg cgctcgacgc
    21361 cataccggcc gcgcttgcag gacaccacct tgcgctcgca aacggcggga gcgagctggt
    21421 ctatacctac gatacgcagg gcccgcgcac cggcattacc ggattgctca ccgacctcgc
    21481 ggcggccggg gtgaggttca aggacctcga taccacgcat agctcgctcg aggacatctt
    21541 cgtcgatctg gtgaggcgcg agcgatgaac tttccggcga tcgtggcgat ctacaagttc
    21601 gagatggcgc gctggggacg cacgttcctg caaagcatcg tctcgccggt gatcgccacc
    21661 tcgctctatt tcgtggtgtt cggcgcggcc atcggctctc gcatcaccga gatcgagggc
    21721 atcgaatatg gagtcttcat cgtccccggc ctcgtcatgc tcacgctcct tacccaaagc
    21781 gtggccaatg cctcgttcgg catctatttc ccgcgcttca ccggcaccat ctacgagctg
    21841 ttgtcggcgc caatctcggc cttcgagatc gtgatcggat atgtcggagc ggccgccacc
    21901 aagtcgatcc tgctcggcct cgtgatcctt gcaaccgcct ggctgttcgt gccgctcaag
    21961 atcgcccacc ctttgtggat ggtcggcttt ctggtgctga ctgccgtgac cttcagcctg
    22021 ctcgggttca tcatcgggct ctgggccgac ggcttcgaga agctgcaact cgtgccgctc
    22081 ctcatcatca cgccgctcac cttcctcggc ggcacgttct attcgatctc gatcctgccg
    22141 cccttctggc ggaccgttac cctgttcaac cccgtggtct atctggtgag cggcttccgc
    22201 tggagcttct acgaaatcgc cgatgtgagc ccagccgtca gcctcattat gatcggggtg
    22261 tttctcgcga tctgcctcgg cgtcgtgtgg tggattttca agacgggcta ccgcctgaaa
    22321 agttgaagcc aatatttccc ggctcggcag agccgtcttc gggaattgtt ttatcgccct
    22381 cccctcgttt ttcgtgtcaa tatcacccat attaggtctg tagttcggca cgttgcagct
    22441 acggaatatt tagcaatgcc ccggaattag accttcgatg agaggttttg tgcaatgacg
    22501 acattcgatt atactgctct tgccgagctt tatccggccc ggagctggaa gcgcagtgca
    22561 ggacgcgtga cctacaagcg attcgaggtt gcggcagagg ccgttcgctt cgccatcgag
    22621 gagcttccgt ccgagtttct tctcggcacc tatcttgagg tcgaagagga aagatttgac
    22681 gcccggcaaa tccgctcgct ttatgagagc aaggactatc ctctcccccg cgggcttgca
    22741 ggcagcgggg aacccaagct gaagccagcg tcccggaagc gcaagtaatg gacgccggta
    22801 gggtcaggac ggcgcccgtg cagacttacg tcgcagagat tgacggtcgg gcgatcttcg
    22861 cgtttcgtgc cgtcgatgac agcaatgcgc aggcgtggct cgacgtcaat gccgtcatgc
    22921 ggcacgcgct cgaaacgctc cacagcaatg gccgccgcat atggaacggg caggcgcaaa
    22981 tccgcgcgcg caaggcgcgc ccccatgagg cgacgatttg gagcaatttg agcggcgagg
    23041 ttgcggtggc ggatcccggt gaagaaacgg atagcatcac ggtgtggctg atccccacct
    23101 cgactgggcg ccactttcgc gacgcaccct aaaaccaacg agccgctccc cgaaggaagc
    23161 ggctctgatt ttaccgagtt cgtctcttgt ccggttaggg actaagcgat ctcgatcttg
    23221 cccacggcgg tcttgccgtt gcggctgtct tgctctgtgt caaaagaaac cttctgacct
    23281 tcgatgagct gggtgatgcc cgcgcgctcg agcgccgttg catgcacaaa cacatcctta
    23341 ccgccatcct caggttcgat aaagccgaag cccttttgcg cgttgtagaa tttcacagtt
    23401 cccttgttca cagggtagtc ctttcgctgt cgtagtgcag ttgtgctcga ggccgagcta
    23461 tgccggcccg aagcgtgttg tcgatgtttg ggagggtctg aacgtgcgcc tggctaacag
    23521 cgaaggcgta acggccacat tgtccggcca accgtcgatt tgactgaaat aagcgtggag
    23581 cgtggctttt gcaaggccaa aggtgcgttc cgcagacgcg tcgcatgcta aggtccgccg
    23641 cgatgaggga acgttcagcc gacaagcaag gcagcgccgc gccggaagcg ccccagctcc
    23701 gtcgcacgat cgggcccaca cagatggcgc tttacgggct cggcagcatg ctcggcgccg
    23761 gcatttacgg actgatcggc aaggctgcgg ggcaggccgg aaacgcccta tggctcgcct
    23821 tcgtcgtcgc gttcgttgcc gcgctgctca cggccctctc ctacgcctcg ctcggctcgc
    23881 gctatccgcg cgccgcaggc gcggcctatg tcaccgagcg cgcctatggc ttctcgctgt
    23941 tgagcttcat ggtcgggatt gcgctcgtgt gctcgggcct tacctcgatc gccacccaga
    24001 gccaggtgtt tgccgccaac atcgccgccc tcttcggcct cgaagacatg ccgctgtcat
    24061 ggctcgcgct cgggtttctc ctgatcctca ccggcatcgt gttccgcggc atccgcgagt
    24121 cgatgtgggt caacgtgctg tgcgcgctca tcgaggctgg cggcctcctg ctcgtcatcg
    24181 tggttggcgc ctcctattgg ggctcggtcg attatctcgc gacccctgcc gtgcccggcg
    24241 gcgaccatgc ggcgctcctc gtcatgcagg cgacggtgct tgccttcttc gcgttcatcg
    24301 gcttcgagga catgatcaat gtcgccgagg agacgcgcga gcctgaacgc acggtaccgc
    24361 tcgggctcat gcttgccatg agcggggcgg ctttcctcta tatcggcgtc gctgtcaccg
    24421 ccgtctcggt cgtgccgtgg ccgaagctcg cagcagcgcc aggtcccatc accgcggtgg
    24481 tggagcgcgc ggctccgtcc atcccgccga tcgtattcac cgtcatcacc ctgttcgccg
    24541 tcgccaacac cgcgctcgtg aactacgtca cggcctcgcg cctgatctac ggcatggcgc
    24601 gccaaggtct tctccctgag cgcctcggca gcgttcatgc cgcgcggcgc acgccgcatc
    24661 ttgccgttgc cgcgctcttt ctcgtgcttg cgccccttgc gctcctcggc acgattacgg
    24721 aactcgccgc ggcgaccgtg ctgctcctgc ttctggtgtt catggtagtc aacggcgcgc
    24781 tcttcatctt gaagcgacgc aagagcgaga aacaagggcg cttcgagatc ccccgcgcga
    24841 taccggcgct cggcgccatg gtctgtctcg ttctgattgt ggtgcgggtg acgacgggcg
    24901 attggcgcgc gccggcgcta gcaggcgccc tcctctttgg cgcgctcgtc ctccacgtct
    24961 tgatgcagat gaggcgctag aaacgctggt cgccccttcg gttcagccgg cgcacgtagg
    25021 tgatggtcgc ctcggggcgc tcggccttca ccttgagcgc cgcgatcaag gcgtcctcgg
    25081 ccttgacgac gagccggtcg cctctaccgt ttacgctgat tccgaccatg aattgggact
    25141 gcacgccgag gctccttgca tgccgggatc ggcgctcaag cctagcaaaa gacggcgcgt
    25201 tcgcccacga ctaaagctgc aagatcgggc cctattgagg gatggagccg gtaaagccgg
    25261 gagccggcgg cggggaggcc gacccaaccg tgcttggctt tggcttgggc ttgggttcgc
    25321 tcttggcctc gacgttgtcg gcgaccgcct cgggcttcgc ctgcttgccc aaggccgccg
    25381 cagcgacccc ggcatcgagg ccataaaggt cgccgaaact ctgcagcttg ccttcggggt
    25441 cggccgacag cgtgaaatag gttgtgtgca ccggcacgcg gttccgaatg gcgacgccgc
    25501 tgttgtagcc cttggcaagc agcttatcga tctcggcctg cggcacgccg ctgtcttcgg
    25561 cgaggatgat cgcggcaacc ttgccagggt tggcgacgcg cgggcagtga tgaccttcgg
    25621 cgcggacctg cagcgacaag agttcgcgct tgatggtgtc gtgcatgtag accgagtgcc
    25681 ggttggggta gaggaatttc accttgccca gcacgttctt cgggcccggc gcctgggtaa
    25741 agctgatcgc gcccatgttc accgtattcc agttgatcga gctcggatta acgcgcttgc
    25801 cgttatagtt cacgttgagt tcgtgtttct tgaggaccga ggtgttgctg ccgaacatgc
    25861 cgcccccacc cctgaggcgc ggcaggagat cctccctgat gatggtcgaa ggcaccgtcc
    25921 attccggatt gaagacgatc gatttcaagt ctgcggagaa gatcggcgtg gcgtagaccg
    25981 gcttgccgac gaccaccttc tccgagtgga tggtcttgcc gttcttgacg atatagacca
    26041 tgaactcggg gatattgatc tgcacgtaga gcgcgccgag atcctgcggc atccagcgcc
    26101 agcgctccat gttgatgagg atcttctgcg tgttgcgcgg cttcgcgccg gcctcggctt
    26161 ccgcgcgcgc cttgagcagt gcttggcgca ggctgtgaaa ttgctcgtgc ttcggatgca
    26221 gcgactggag atagagagcg ggctcggccg catcggcgat ctcggcgagc acggtcttcg
    26281 gatcgcggaa ggtcggcacc atatcgttat tcttgagctt gtcagggtcc gtcctgccgc
    26341 cgcgcgcgtg acgcgcgtat tttaggatcg cgagatcaag ctcaatctcg ccgcgtgcct
    26401 gctcatcgag cgagggggga agagtgctgg acgaaggcag atcgaacgcg ccgctcgcga
    26461 gcccccaatt gtcggcctcg aggatctcat cgatggcggc gaggccctcg cgcgagaatc
    26521 ccatatccgt catccagacc ggttcgctcc gcccgttata gaaagcttcg agcgcggcga
    26581 tgtcctcggc cgaagccttt ccgcgaaagg cgggatcggc gagcttggtg cggatcgaag
    26641 cgacgatcgg cgtggccgcg gccgggatgg gcacggtttg cactgtcgtc gtgctcggcg
    26701 gcgttccctc agagtgcccg gcatcgtcgg cgggcgttgt cgcctgaggg gccggctcgg
    26761 ccggagccgg catcgagcca ttttgacccg cggcttgatc ggccggcgct ggtccgctcg
    26821 cgttcgggtc gtcagcgtct tccgcggccg ccgtctcttg cgcgaaagcc gacgttccga
    26881 gaactgcacc gaataggaga attggagaag ccgcagcaag cgcggctacc cagaaccgac
    26941 cacgcatctc ccaacccctc gtaccctgct ctaaggttaa tccgagaccg ggccgagggc
    27001 caacaggatt tcgcccgtgg atgggcgcct cagtcaatcg cggcggggag cttcgcgccc
    27061 gttttgagag gcctctcaag gcgctggcgg cggcgcctca ggcccattga agttgccgtt
    27121 cgatacccta tactcgatgt gagaatagct ccggaggccg gccttgcgcc ggatgtttta
    27181 acaacccata tatgctcatt atgagcatta acgttgacgg agatcgttcg atgacgcggc
    27241 ttcaacacag cgcttcgcat agcgcgcgca agactgagac cgcacttccg ccgagcctgt
    27301 tgcccgacct cgcctacacg cccgaagtcg cgcgggagac cgcgcatctc tacgagcgcg
    27361 tggcgcgcgt catcccgccg atcgagtggc cgggcttcgc gccctacatc aaggccatca
    27421 acgagcttaa gcgcgcgcgc aacgcagtcg tgctggcgca caactacatg acgccggaaa
    27481 tcttccattg cgtcgccgac gtggtgggcg actcgcttca gcttgcgcgc gaggcggcca
    27541 agaccgatgc cgagatcatc gtccagggcg gcgttcattt catggccgag acctcgaagc
    27601 tgctcaatcc cgacaagaag gtgctgatcc ccgatctgcg cgccggctgc tcgctcgccg
    27661 cctctatcac cggcgccgac gtgcgcgcct tgcgcgaggc ctatcccggc gtgcccgtag
    27721 tcacctatgt gaacacgtcg gccgaggtta aggccgagtc cgatatttgc tgcacctccg
    27781 cgaacgcggt tcgcgtcgtg gagtcgctcg gcgcgaagcg cgtgatcctt attcccgacg
    27841 aatatctcgc caaatgggtc gcgacccaga ccccggtcga gatcatcacc tggaagggcc
    27901 attgcgaggt gcacgagcgc ttcaccggcg aggagctgcg tgcctatcgc gagtccgatc
    27961 ccggcgtgaa gatcatcgcg catccggaat gcgcgcccga cgtgctcaag gaagccgact
    28021 tcaccggctc gacctccggc atgatcaaat gggtcaagga gcaccagccg tcgaaggtgc
    28081 tgctcgtgac ggaatgctcg atgagcgaca atgtggccgt cgaggcgccg aacgtcgcgt
    28141 tcgtgcggcc atgcaatctc tgcccgcaca tgaagcggat cacgctgccg aagattctcg
    28201 agagcctcgt cttcctcaag gaggaggtca cgatcgatcc tcgcatcgcc gagcgtgcgc
    28261 gcatgtccgt cgagcgcatg atcaacctca accattgacg tcggctgccg accacggcac
    28321 atccgcgatg acagtacatt tcgagttctc cgcaaaagcg catgaagggg tcgtggtgat
    28381 cggcgccggg cttgccgggc tgttcaccgc gctgaagctc gcgccactgc ccgtcaccgt
    28441 cgtttcggcg gcgcggttcg gcgagggggg ctcgagcctg tgggctcaag gagggatcgc
    28501 cgcggcgctt gccgaaggcg acacggccga agcccatgcc gccgacacca tccgcgccgg
    28561 agccggcatc gtcgatgaaa ggatcgcgct cctgatggcg cgggaagcgc gcgagcgcat
    28621 cgaagacctg ctcggctacg gcgtgccctt cgacaaggac cttgaaggcc atctcgcgct
    28681 cggccgcgag gccgcgcacg gccgcaaccg cattcttcac gtcaagggcg acacggccgg
    28741 acgcgccatc atgcacgcgc tcacggaggc ggtgaggaag atgccgtcga tcaccgtcct
    28801 cgaaggtctt gccgcgcgcg atcttgtgct caagcacggc agcattgtcg gcatcgagct
    28861 tgcgccggcc gatgacccac gcgcgagcgg cttcattctt ccggccggcg ccgttgtgct
    28921 tgccaccggc ggcgcgggcc agctctattc cgtgaccacc aacccgcgcg aggcacgcgg
    28981 tgacggtatt gccattgccg cgcgggccgg tgcggcgatc gccgatgcgg agttcgtgca
    29041 gtttcatccg accgcgatcg atatcggccg cgaccccgcc ccgcttgcga ccgaagcgct
    29101 gcgcggcgaa ggcgcgcttc tgatcaacgc acggggcgag cgcttcatgc gcgcggtcca
    29161 tgacgacgcc gagctcgggc cccgcgacgt cgtggcgcgc gccgtgcacc gtgaagtgat
    29221 gagcgggcgc ggcgccttcc tcgactgccg tccgctgggg acgaagctgg tccagcattt
    29281 cccgacggtg gtcgccgcct gtcgcgccgc cagcatcgat cctgtgcgcg agccgatacc
    29341 ggtcgctccg gccgcccatt accacatggg cggtatcctg accgacgcat ccggacggag
    29401 cacggtcgac gggctttggg cttgcggcga agtggcctcc accggcgctc atggtgccaa
    29461 ccggctcgcc tccaactcgc tgctagaggc ggtggtgttc ggcgcccgcg ccgccaagga
    29521 tatcgcttcg tcttggcgtc ccatgagcct caaggcggta cggcaagagc cgtaccgctc
    29581 cgttgcggcg gagcgcggca gggccgagag cgccgagcgg aagcttcgcc tggccatgac
    29641 ggaaggggcc ggcgtcatcc gcgacgggac ttcgctgcga aacgcgcttg ccactatcgc
    29701 ggcgcttgag aaggtctacg acccgcgtct cgccaacatg ctgaccaccg cgaaactgat
    29761 tgccgcagca gcgctcgagc gcaaggagag ccgcggcgcg catttccgca gcgactaccc
    29821 gctctcggac gagcgcctcg ccaggcgcag cttcctcacg ctcgaaaagg ccgaagccat
    29881 ggccggggaa gcgcgcgaga cggccgcgcc ggcacgcgga cgtcgctccg ggctggcccg
    29941 tgcagcgctt cctgcatgag cgcgaaatcg cccttcccgc ttgccttgcg gccgcttctc
    30001 gtcgaacgcg ccgttctggc cgcgctcgac gaagaactcg gcgcggcggg ggatatcacc
    30061 accgacgcga ccattgcggc cgacgccacc ggcgaggccg cgattgtcgc gcgccaatcg
    30121 ggcgtggtgg cggggctcga ccttgccgag gccgccttcg aggccctcga ccccggagtt
    30181 cggttcacgc ggatcgtcgc ggacggcggc agggtcgccg agggaggcaa gattgcgacc
    30241 gtcgccggca agacgcgcgc gctgttgagc gcggagcgga cggcgctgaa cttcctcgga
    30301 cgattgagcg gcatcgccac gctcaccgcg tcctacgtca aggcggttga aggcacggcg
    30361 gcgcgcatcg cctgcacgcg caagaccacg ccgggactgc gcgccttgga gaaatacgcg
    30421 gtcagggccg gcggcggcat caaccatcgt ttcggccttt atgacgcggt gttggtgaag
    30481 gacaaccata tcgccgctgc cggcggtctc gccaacgcgc tcgcgcggct gaaatcacgc
    30541 gcagggcatg cggtccggat cgaggtcgag gtggatacgc tcgatcagct cgcagacgcg
    30601 ctcgcgtatc ccatcgacgc cgtgctgctc gacaatatgg acctcgacac tcttcgcgaa
    30661 gcggtgaaat tggctgcagg acgggtcgtg accgaggctt ccggcggcgt gacgctagaa
    30721 acggtgcggg aaatcgcctt gaccggcgtc gacgtgatct cggtcggcgc gctcactcac
    30781 tcgccgcgaa acctagactc atcgctggag tggcagaact agagtcgtcc tccgcaaccg
    30841 ccgagacaag ggaaacggcg ggtgccagcg tgaacagcga gggcggagac tccggcggct
    30901 gcggctcgct cggctgcggg gccgcggacg cttcgacgag ataagggtgc tccttgcgct
    30961 tcttgtagaa gatgtggcgg ccgatcgtat cgatcctgct catgtcgccg gtccagttcg
    31021 gccgcacgta attggcgtgg taataggtcg aatagccgac ctcgggaagc caaagctcgc
    31081 ccttggtgat gcgtctcgcg atatcctgcg cccgggccca ttgcgcatcg ttgcgcggat
    31141 tgtcggtctt gccgtcgcag gtgaaggaga actggcagcc tggcgccatc tggccctgat
    31201 agacgacgcc gcagatcgtc tcggggaaga gcgggcttct cacgcgattg aggatcactt
    31261 gtcccaccgc gacctgaccg cgcatcggct cgccgcgcgc ttcgaaatag atggcaatgg
    31321 cgaggcagcg ccgctcgcgc gcctcgaact cggcctggct ttcgccttgg tagcggaaat
    31381 tcagagcctc tggcgtcggg tgataggcga tatccgcggg cgaaggccgc aacgaatccg
    31441 catcgaaggt cgaagctgcg gccaacatca gcgaggtgtc ggggccgctt gcgggattgc
    31501 tgagggcgat taccgaggtc gtcggtttga gccggtcgcc cttgatcgcg ccattgacct
    31561 cgccccggcg cgagacggcg ccggtcgtgg ccggatcgcc ggtgaggctc gcggcgatga
    31621 gctgcttgcc ggccgaggtc accgtgttgc gcgcgagata ttggtcggcc cttggctcct
    31681 tgcctttggc gcggaagaga ggggcggcct cgcgcggcaa gcgctccggc gcggcttctg
    31741 cccggcgcgt cagagactga tccgagccct tgccagcaag ataaagctcg acttcgaaag
    31801 cgaccgggtt gccgggcacg gcagcgacat agccgagcgc aatgaggggg agcggcacga
    31861 gcgcagccac ggtgtgccac atccgacgag gcaggcgtcg gcccatgttc cctccagttc
    31921 cccgtagtcc cgcgagactt gcaggcgacg cgttcgcctg ccttcccgct ccgtcccaaa
    31981 ccgcaggcat gagcctacga aatggggcgt ccccaattct tcaccaggca aagatggtgg
    32041 gaattcaata ttttagttgt aagtccccca ccggttttgg cccggaaatc ccgtctggtt
    32101 accttaaccg tgcccgataa attagagcaa aatgtggtcg agggcaactt aaccctagta
    32161 gtcaggtgga agcgctaagg atcggttacc gtagcgccca gggccgcctg ggcggcggcg
    32221 aggcgggcaa ccggcacccg atagggcgag caggacacat agttgagcct aattttctcg
    32281 cagaatccta tggtccgcgg gtcgccgcca tgctcgccgc aaatgcccat cttcacgtcg
    32341 gggcgcacgc ggcgcccgcg ctcggcggca atctgcatca attcacccac agccttgtcg
    32401 agggtgacaa agggatcatg ctggatgatt cccttgtcga catactcgcc caagaatcgc
    32461 ccggcatcgt cgcggctgag cccgaacgtg gtctgggtca ggtcattggt gccgaaagag
    32521 aagaactcgg cgctctcggc gatctcgccc gccatcaggc aggagcgggg cagctcgacc
    32581 atggttccca ccgaataggc aagattggtc ccacgttcct tttcgaccgc gctggcaaca
    32641 ctggcgatgc cccctttgac gatctcaagc tcgcccttgg tgacgacgag cggcaccatg
    32701 atctcgggga tgacgcggtc atgcagggcc tggctcgcct cgatggccgc ctcgaaaatt
    32761 gcgcgggcct gcatctcgac gatctcggga tagctcacga ggagccgcga cccccgttgt
    32821 ccgagcatgg ggttgaactc ggtgagggct gcgatccggc ggcgcaatcg cgcgacgctc
    32881 acgcccatgc ttgccgcaac ctcggcgatg atgtcctcgc tatgaggcag gaattcatgc
    32941 agcggaggat cgagcaggcg aatcgtcacc ggcagtcccg ccatgatctc gaaaagctcg
    33001 atgaagtcct ggcgctgcgc cggcaaaagc ttggcgagcg ccacgcgccg cccgtccgga
    33061 tcttcggcaa ggatcatctc gcgcatggcg agaatgcggg tctcctcgaa gaacatatgc
    33121 tcggtgcggc agagcccgat gccttcggcg ccgaaggccc gcgcctggcg cgcatcggcc
    33181 ggcgtgtcgg cattggcgcg caccttcagc cggcgaaagc cgtcggccca ctgcatcagc
    33241 gtgttgaagt cgtcggataa ttccgcctgg cgcatgggca cccggccttt gatgatctgg
    33301 ccggtcgagc cgtcgatggt caccacctgg cccttgtgca agagcacgcc tccggcagag
    33361 agggtctcgc gctcgaggtc gatcacgacc gaagaagcgc cgaccacgca aggcctgccc
    33421 atgccgcgcg ccacaacggc ggcgtggctc gtcatgccgc cgcgcgtggt gagaatgccg
    33481 accgcggcat gcatgccctg aacgtcctcg ggcgaggtct cgacgcgggc gagaatcacg
    33541 gtatggcctt gggccttgag atgcaccgcc tcgtcggcgt cgaacacgag ctcgcccgaa
    33601 gcggcgccgg gcgaagccgg caggcccttg gcgagaaccg tcttctcggc gtcggggtcg
    33661 agcgtcggat gcaggagctg gtcgagctgg ccggcatcga tgcttcgcac cgcctcttcc
    33721 cgggtgatca ggccctcttc ggccatatcg acggcaatct tgagcgcggc ctgcgtcgag
    33781 cgcttgcccg agcgtgactg caagatgaag agcttgccct cttgcacggt gaattcgatg
    33841 tcctggatat cgcggaaatg agactcgagc tcggcacacg ctgcttcgag cgttgcgaac
    33901 acgtcgggca tgagggcctc gagcgaaggc cggctcgcat tggcggcgcg ccgggccgcc
    33961 tcggtcaggg actgcggcgt gcgtatgccc gcgaccacgt cctgcccttg cgcgttgacc
    34021 agaaactcgc cgtagagctc gcgcgcgccg gtcgagggat tgcgcgtgaa cacgacgccg
    34081 gtcgcgctcc gctcgcccat attgccgaac accatggcct ggatggtgac ggcggtgccc
    34141 caatcgtcgg gaatatcgtg aagccggcga taggcgatgg cacgggcgtt ctgccaggac
    34201 ccgaacacgg cggcaatcgc gccccaaagc tgggcgtgga tgtcttgcgg gaacggcgtg
    34261 ccgagctcgg ttgcgacaag cgtcttgaag cgggcgatca gctcggccca atcctcggcg
    34321 gaaagctcgg tatcgagttc gaagcctttt agattcttga cgttctcgag gatctcctcg
    34381 aacagctcgt ggtcgatgcc gagaaccacg tcggcataca tttggatgaa gcgccgatag
    34441 gtgtcgtagg cgaagcgccg gtctccggat cggcgcgcca gaccctcgac ggtgcggtcg
    34501 ttgaggccga gattgagcac ggtatccatc atgcccggca tcgaggcgcg cgaaccggag
    34561 cggaccgaga cgagcagcgg attgtcctca tcgccaaatc gtgcaccgac gatctcgccg
    34621 accttgtcga gcgcggcatt gaccatcggt ttcagctcct cggggagctt tcgcccgagc
    34681 tcgtaataga cggcgcagag ctcggcggag atggtgaagc ccggcgggac gggaagcccg
    34741 agccgcgaca tctcggcgag ataggcgccc ttgccgccga gcctgtcccg gcccgcggcc
    34801 gagccgtcgg cacgcccgcc gccgaagctg tagacccatt tcggcgaggt cttgagcgcg
    34861 ggttccgcct tggtcatcgc gtggtccatg atcggtccgc cgttgtagcg gtcccccgaa
    34921 cttcacacaa acggatgcag gcgggaatgg tttaagggcg tactcccgaa ggagcgcgaa
    34981 atcggcgatc ggcgatgaag tcgccggtac cgatcagcct ggcgcggggc atttccttga
    35041 gcgcataggc ccaggcctcg acgctgccgc ctttgtcgag tgtgaccttg acggcgatgc
    35101 ggtgaaacag gccttcggcc tccggccatt cccgctcctc gccttgcgcg ggggttacgc
    35161 cttcgtattt gtcgagatcg atgagaaacc tcgaggcagg cctaagaaca aacacgttcc
    35221 cgatcacgcg gtatttctgt tcgggcgcga agaaggcgcc gggccaagaa ccgaaatcgt
    35281 aaagccggcc cggtgtcgag cctctgccga tattccgcgc agcaactctc aggcgatgcg
    35341 ccatcggatg cgaactctcc gctctcagcg tgccatagac aaagagatga tccggcatgt
    35401 ccttcccagt cactgcggca gcgtcaggct gccgtcctta cccaaatccc aatgcgggtt
    35461 gtaggcgatc tccccaaacg gcaatgcaga ggtgatgagc gtgacgttgg ctgacaccaa
    35521 ccctcctttc gtcattgccg ggcttgatcc ccggatcaag tccggggaca ggctccggca
    35581 atccattcca atggcgcttc agttcgctga aatgtaacgg catggatgcc cggatcaagt
    35641 ccgggcataa cgagggaagg ttttcctctt ttcgacattg ccgggcttga tccccggatc
    35701 aagtctgggg acaggctccg gctatcgatt ccaacggcgc ttcagcccgc tgaaatgtaa
    35761 cggcatggat gcccggacaa gcttgcgccc gggccggcga agccgggccc gggtgtccgg
    35821 gcatgacgag ggaaaggttg ctcatccttc gatcttggag aaatcggcga cggtaagcgt
    35881 cgttgcgcga atacggttca agaggcgcaa acggttggcg cgcagatcgg gttcctgcgt
    35941 gttgacggtg acgaagtcga aaaaggcatc gacggggacg cggagcttgg ccatctccga
    36001 cattgcgctt tcgaaatcct cgcgcgcaat tgcggaggga gcggcccgtt caacctcgtt
    36061 gacggcctcg gcaagggcct tctcctcggg gcgcttgagg agcgccgagt caggtgcctg
    36121 gtcgtaggcg atgccgtcct tcttctcctc gatgcgcaag atattgatcg cgcgcttggt
    36181 gccggtgagg agatgctcgc cgtcctcggt gtcgaggaag cggccgagcg cgtccacgcg
    36241 gcggacgatc atgaggaggt cgtcgccgcc aagggcaaac acggcgtcga ccagatcgtg
    36301 acgtgcaccc tgctctctca gatgcacttt gagccggtcg gcgaagaagc atagcaaatt
    36361 tcttgcagcc gcctcgttca gattcgcatc gcccctagct tgttggcgaa actgcgccat
    36421 gagcggcagg cgcacgccgt tctcaagcag gatgcggatg acgccgaggg cggcgcggcg
    36481 aagcgcgtaa gggtctttgg accccgtcgg tttctcgtcg atcgcccaaa accccatcag
    36541 cgcatcgagc ttgtcggcga gcgccacggc aatcgcaaca ggttcgcgcg gcacctcgtc
    36601 ggacgggccg aggggcttgt agtgggcggc aatcgcgttg gcgacggccg gatcctcctc
    36661 ttggtcgagc gcgtaatagc ggcccatcac cccttgcagc tcggggaact cgcccaccat
    36721 gccgctcaag agatcggcct tggccaggat cgccgcgcgt tcggcaaggt ctggatcggc
    36781 gccgacgaga ggggcaagct tgcgcgcgag cccgcgcacg cgctcgatcc gatcgtgctg
    36841 gctgccgagc ttttcgtgaa agatgatcgc gtcgagcttc ggcacgcgct cctcgagcgg
    36901 aaccttgcgg tcctgatcga agaagaactt ggcatcggcg agccttgctc tgatcacgcg
    36961 ttcgttgccg gcgacgatcg ccttgccgcc gtccttggct ttcagattgg aaaccacgat
    37021 gaaccggctg gcgagcttct ctccctttct gagcgagaag catttctggt gcgccttcat
    37081 ggcggtggcg agcacctccg gcggcactga caagaaagct tcgtcgaacc tgcccatcag
    37141 cggcacgggc cattcggtaa gcccgacgtt ctcttgcagc agaccctcat cctcgacgag
    37201 cgtgagcttc tcattcctgg cgagagcgcg tgcgccatcg aggatcagtt tcgcgcgttc
    37261 ctcgccgtcg agaatgactt tggcggcgcg gagcttcttc acgtaatcgg caaagccttt
    37321 cacggcgaag ggctttggcg ccatgaagcg gtggccgcgc gtctccttcc cgctcctgat
    37381 gccgtcgacc gcgaacggca cgaccttgcc gtcgagcaag cagagcattg aatggagcgg
    37441 ccgcacccaa cgaagtttgc ccgagcccca gcgcatggat ttcggccagg ggaaagtctg
    37501 cacgatggcg ggaacgatct cagcgactac ctctgccgtc gcccggccgc gtttttcgat
    37561 cgtcgcgacg tagaaggcgc ccttcttgtc ctcgcgccgt tcggcctgat cgatggaggc
    37621 aagacccgca gacttgagga agcccttgat ggcggtttcc ggcgcatcga cgcgcgggcc
    37681 cttcttctcc tcgcgcacgg cgggcgagcg cgccggaagg ccgtcgacga cggccacgag
    37741 ccggcgcggc gtggcgaagg tccgcagctc cttgtattca agccccgcgc ctgagagcgc
    37801 ctgcgcgagc aggcgcgcga ggtccgcgcg cgcgcgcacc tgcatgcgcg cggggatctc
    37861 ctcggagaac aattcgagca gaagctcagc catcggcgcc gccagcctcg gtcttgagcc
    37921 atgcggcgca acaggctttg gcgaggtcgc gcacgcgcaa gatataggcc tgccgctccg
    37981 tcaccgagat cacgccgcgc gcgtcgag
//
LOCUS       DASUQP010000225         9203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1610077, whole genome shotgun sequence.
ACCESSION   DASUQP010000225 DASUQP010000000
VERSION     DASUQP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610077"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..143)
                     /locus_tag="VFY74_10330"
     tRNA            complement(68..143)
                     /locus_tag="VFY74_10330"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Lys,seq:ctt)
     gene            complement(263..1420)
                     /gene="rodA"
                     /locus_tag="VFY74_10335"
     CDS             complement(263..1420)
                     /gene="rodA"
                     /locus_tag="VFY74_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX5932129.1"
                     /translation="MKALFAEGLERPQVKLKQKLLALNWPFLLLLTAITLVGVAALYS
                     VAGGSLEPWASRHVVRYCIGLALLFTVALSDIRFWLRVAYPLYFVVLIMLAAVLLIGV
                     ESGGATRWLGWGEFSFQPSEIMKIALVLALARYYQWLPPSQVSWPYAVLPPLAMVGVP
                     ILLALDQPDLGTAALFGIVGGGLLFLAGVSWIYFIAAFVGVIVVLPHVWARLHDYQQE
                     RILTFIDPERDPSGSGYHILQSKIAIGSGGFAGKGFMQGTQAQLNFLPEKHTDFIFTM
                     FSEEMGFIGAAVLLVLYLLVLLFIAYMALRCRGTFARLVAAGMGLCLFAYVFINVAMV
                     TGLVPVVGVPLPLVSYGGTSMLTMMVGLGFVLNAHINREARLRREELGPFW"
     gene            complement(1417..3264)
                     /gene="mrdA"
                     /locus_tag="VFY74_10340"
     CDS             complement(1417..3264)
                     /gene="mrdA"
                     /locus_tag="VFY74_10340"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5932130.1"
                     /translation="MKPRDDALKLRHRFSRRAVLIGAAQTGLFGLLLWRLRQLQILDS
                     PEYALLSDDNRITAHLVAPARGAIYDRLGRAIAEDRENFRVIVIPAFCKDLAATLDAI
                     SEIVPVSAADKDRVLRAARRQSGYFPVLVTEGLQWRQFALLSVLAPQIAGVRTDRATY
                     RRYARAPSMAHVVGYVGLADKAEIGEHPVMRVPGFRTGKAGIEKGLDPELRGQPGTIS
                     YEVDAHGRVVRELGATASVRGKDLVLTVDQELQEIAHKRIEGLRVASIVVLDVATGGI
                     VVMASFPSFDPNEVSFKVDLARWKELAKNVDHPLENRALRGVYPPGSTFKVVTALAGL
                     EAGVISPQERMHCPGGYPFGRHYFRCWKRHGSVYLHEAIKQSCDVYFYETVKRVGIDR
                     LAVMSRKLGLGQIHEIGLAGQKPGIIPDTKWKRRTLGQPWYPGETISCGIGQGYVSTT
                     PLQLAVAAARIATGRAVRPRLIAASEAEPEAAPLDIDPLHLQLVRAGMAGVVNEPGGT
                     AVRSVLALPNIKMAGKTGTSQVISAKNQGFLKGWYGETHALFIAYAPADAPRYAAACV
                     VEHGGGGSRAAAPVVRDVMTEVLLRDPAIKTALLASNLEAPKAAKERKR"
     gene            complement(3261..3761)
                     /gene="mreD"
                     /locus_tag="VFY74_10345"
     CDS             complement(3261..3761)
                     /gene="mreD"
                     /locus_tag="VFY74_10345"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5932131.1"
                     /translation="MSAYLPALSVFAAALATAIPWGLPAGATFILPMLLMAMVFCWRT
                     LPDTAFPVYLALLLGLLTDILSGGPLGFWALMALIAANAGERAPSPGDSQDRNRLWLV
                     WAGVAVSIGVLSWLLGSLYFLRWIDAWPIALGVGVSILLFPVVLRGVVRIKRGRFRHK
                     LYRGLT"
     gene            complement(3758..4711)
                     /gene="mreC"
                     /locus_tag="VFY74_10350"
     CDS             complement(3758..4711)
                     /gene="mreC"
                     /locus_tag="VFY74_10350"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5932132.1"
                     /translation="MLNFALIFLCIVLLVLSRIGHAALTEARDSVVDMTAPLLEATST
                     PVMMARHAASQVGSYVGTFSEIDRLKVENEKLKQWEWRAKLAERNVAQLRALLNAVDE
                     PALHFTTGNVIADARGPFLQSALINLGRDNGVRVGYAVINGDGLVGRTVEAGASVTRI
                     LLLNDLSSRVPVLVGHAGVRAIASGDNSAALRLDFLPEGALLHPGDEVFTSGSDGVLP
                     RGLRVGVVTGSPGAYRIEPHADLSALDVVSVLFFDAPALTRTDPPTASLNRALSASSD
                     DSKPPAPVPTGSVEQPEMTGAIKQAGTPGTPASESVAQTQR"
     gene            complement(4869..5909)
                     /locus_tag="VFY74_10355"
     CDS             complement(4869..5909)
                     /locus_tag="VFY74_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5932133.1"
                     /translation="MFSRLLGFVSADMAIDLGTANTLVYVPGRGIVLDEPSVVAVTQQ
                     RDGDRKVIAVGYEAKSMLGRTPSKIETIQPLRDGVIADFNAAEEMIKHFIRRVLKRPL
                     FSSPRIMICVPASATPVERRAVHEAGLSAGARRVYLIEEPVAAAIGAGLPITEARGSM
                     IVDIGGGTTDIAVLSMGSVLYSRSVRSAGNAFDEAIVNYVRFKYNLLIGATSAETIKK
                     ESGCAIAKANGERMPIHLKGRDLHRGFPADIALTPDDIPQALNLPIQQIVGGIRQALA
                     DVPPELTADICDSGIYLTGGGALLDRLDARFSQETGVKFKIAEDPLRCVVRGTGKALE
                     QLDTMSDFLVKP"
     gene            complement(6308..7891)
                     /locus_tag="VFY74_10360"
     CDS             complement(6308..7891)
                     /locus_tag="VFY74_10360"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX5932134.1"
                     /translation="MESRKDSAKAKGRSERDRVVIFDTTLRDGEQCPGATMTFEEKLE
                     VAEVLDEMGVDIIEAGFPIASEGDFNSVQEIARRAKKAVICGLARAQFKDIDRCAEAI
                     KPAARGRIHTFIGTSPLHRKYQMSIDEKEVLERVTASVTRARSYTDDVEWSAMDATRT
                     EHDFLCRCVEAAIKAGATTVNIPDTVGYATPDEFAALIGMILIRVPGAEKIVFSTHCH
                     NDLGLAVANSLAGVGAGARQVECAINGLGERAGNAALEEIVMAIRTRGDAMPFYTGID
                     TTMLTRASRLVSRVTAFPVQYNKAIVGRNAFAHESGIHQDGVLKHAETFEIMTPESVG
                     LRESSLVMGKHSGRHAFREKLKELGYELGDNAFEDAFVRFKALADRKKHVYDEDLEAL
                     VDQEIASAHDRIKVVALTVIAGTGGPQKATITLDIDGKQQTCEQTGDGPVDATFRAIK
                     AIVPHEARLPLYQVSAVTEGTDAQAEVSVRLEADGKTVTGRGSDTDTLVASARAYVSA
                     LNKLLAREARRKPESMLAS"
     gene            complement(8184..8834)
                     /locus_tag="VFY74_10365"
     CDS             complement(8184..8834)
                     /locus_tag="VFY74_10365"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX5932135.1"
                     /translation="MDSVLNIFLLELAALFPVVNPPGSALVFLGMTGNVTHEVRRLIA
                     WRVARNAFFVLVGSLSPGALILRFYGISIPVLRVAGGIIVAVAGWKLLTEGSQKELEA
                     STDGRRPANPLDQAFYPLTLPLTTGPGTIAVVISLGLSRGSYTSSTSEADCRRHFCVF
                     RVRGPRPAPVGAGRHQYRRAALGLHPILPRNPDSLVRRKRVVAVRDAHPAPPVPPT"
     gene            complement(8893..>9203)
                     /locus_tag="VFY74_10370"
     CDS             complement(8893..>9203)
                     /locus_tag="VFY74_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HEX5932136.1"
                     /translation="GLLFLAGGIQLVRLRSASGAAFNQGKTLVIAGCLLAFLVWFLGF
                     MVVAGEWFDMWQSKDWNGQDAAFRFYVTVLGVLIFVSLPDGDLAPPADSPKRRRGDFM
                     "
BASE COUNT         1722 a         2918 c         3056 g         1507 t
ORIGIN      
        1 tgttgcccca tatgttgccc ggaagaaaac ctcggtctcg gttattctgc taatgttttg
       61 attttcttgg tgggcgcaca aggactcgaa ccttggaccc gctgattaag agtcagctgc
      121 tctaccacct gagctatgcg cccgtttttg tcgcctctgc cttgccaagg cgcagcgtgg
      181 gatgaccacg tgagggtgct ccccttagca ctgccccgct tgccctgtcc aggcgccgct
      241 tcgccggtgc ctgcgccaag gctcaccaga acgggccgag ttcctcccgc cttaggcgcg
      301 cctcgcgatt gatgtgcgcg ttgagcacga agcccaggcc taccatcatc gtcagcatcg
      361 aggtgccgcc ataagagacg agcggaagcg gcacgcccac cacgggcacg agccccgtca
      421 ccatcgccac gttgatgaac acataggcga agaggcagag ccccatgccg gcggcgacca
      481 gccgcgcgaa ggtgccgcgg catcgcagag ccatataggc aatgaacagc agaacgagaa
      541 ggtagagcac gagcagcacg gcggcgccga tgaagcccat ctcctcggag aacatcgtga
      601 agatgaagtc ggtgtgcttc tcgggaagga aattgagctg ggcttgcgtg ccctgcatga
      661 agcccttgcc ggcgaagccg cccgagccga tggcgatctt cgactggagg atatggtagc
      721 ccgagcccga cgggtcgcgt tcggggtcga tgaaggtcag gatccgctcc tgctggtagt
      781 catggagccg cgcccagaca tggggcagca cgacgatgac gccgacgaaa gcggcgatga
      841 aatagatcca gctcaccccg gcaaggaaga gcagtccgcc gccgacgatc ccgaacaatg
      901 cggcggtgcc gagatcgggc tggtcaaggg caaggaggat gggcacgccg accatcgcca
      961 gcggcggcag gacggcgtag ggccaggaga cttggctcgg cgggagccat tggtagtagc
     1021 gcgcgagcgc gagcacgagc gcgatcttca tgatctccga gggctggaag ctgaactcgc
     1081 cccaccccag ccagcgcgtg gcgccgccgc tttcgacgcc aatcaggagc accgcggcga
     1141 gcatgatgag gacgacgaag taaaggggat aggccacgcg cagccagaag cggatgtcgg
     1201 aaagcgcgac ggtgaagagg agcgcaaggc caatgcagta gcggaccacg tggcggctgg
     1261 cccagggctc gagcgagccg cccgcgaccg agtagagcgc ggcgacgccg accagcgtga
     1321 tcgcggtgag gagcagcagg aaaggccagt tgagcgcaag cagcttctgc ttgagcttga
     1381 cctgaggccg ctccaagcct tcggcgaaca gcgctttcat cgctttcgct ccttcgcggc
     1441 ctttggcgct tccagattgc tcgcaagcag cgccgtcttg atggcgggat cgcgcaagag
     1501 gacctcggtc atgacgtcgc gcacgaccgg cgccgccgcg cgcgatccgc caccgccatg
     1561 ctcgacgacg caagccgcgg catagcgcgg cgcatcggcg ggcgcgtagg caatgaacaa
     1621 cgcgtgggtc tccccgtacc agcctttgag gaaaccctgg ttcttcgcac tgataacctg
     1681 cgaggtgccg gtctttcccg ccattttgat gttggggagt gccagcaccg agcgcacggc
     1741 cgtccctccc ggctcgttca ccacgccggc cataccggcg cggacgagct gaagatgcag
     1801 cggatcgatg tcgagcgggg cggcctctgg ctcggcttcc gaggcggcaa tcaatcgggg
     1861 gcgcaccgcc ctccccgtcg cgatccgcgc ggcggcgacc gcgagctgaa gcggcgtggt
     1921 cgagacatag ccctggccga tgccgcagct gatcgtctcg ccgggatacc agggctgccc
     1981 gagtgtcctt cgcttccact tcgtgtcggg gatgatgccc ggcttttgcc ccgccaatcc
     2041 gatctcgtgg atctgcccaa gcccgagctt gcgcgacatg acggcaaggc gatcgatgcc
     2101 cacgcgcttc accgtctcgt agaaatacac gtcgcaggat tgcttgatcg cctcgtgcag
     2161 gtagaccgag ccgtggcgct tccagcagcg gaagtagtga cgcccgaagg gatagccgcc
     2221 gggacaatgc atgcgctctt ggggggagat gaccccggcc tcaagccctg cgagcgcggt
     2281 gaccaccttg aaagtcgagc cgggcggata gacgccgcgg agcgcgcgat tctccagggg
     2341 atgatccacg ttcttcgcga gctccttcca tcgcgcgaga tcgaccttga aggagacctc
     2401 gttcggatcg aaggagggaa acgacgccat gacgacgata ccgccggtcg ccacgtcgag
     2461 cacaacgatg gaagcgacgc gcagtccctc gatgcgctta tgcgcgatct cctgcagctc
     2521 ttgatcgacg gtgagaacga ggtccttgcc gcgcacgctc gccgtggcgc caagctcgcg
     2581 caccacgcgg ccatgggcgt cgacctcgta gctgatggtt ccgggctgtc ctctgagctc
     2641 ggggtccaga cccttttcga tcccggcctt gccggtgcga aatcccggca cccgcatcac
     2701 cgggtgctcg ccgatctcgg ccttgtcggc caaaccgaca tagccgacca catgggccat
     2761 gctgggggcg cgggcatagc ggcgataggt cgcccggtcg gtgcgcacgc ccgcaatctg
     2821 cggcgccagc acgctcagca aggcgaattg acgccattgg agaccctcgg tgacaagcac
     2881 cgggaaatat ccgctttggc ggcgtgcggc ccgcaacacg cggtccttgt cggcagccga
     2941 gaccggaacg atctcggaaa tggcatcgag cgtcgccgcc aaatccttgc agaaggccgg
     3001 gatgacgatg acgcggaaat tctcgcgatc ttcggcgatc gcccggccca accggtcgta
     3061 aatggcgccg cgcgcaggcg ccacgagatg cgcggtgatg cgattgtcgt cggagagcag
     3121 cgcatattcc ggggagtcca gaatctgcaa ttgacgaagg cgccagagga ggagtccgaa
     3181 caggccggtc tgcgctgcgc cgatcaagac cgcgcggcgc gagaagcgat gccgaagctt
     3241 aagggcgtcg tcgcgcggct tcacgtcaaa cctcgataga gcttatgccg gaatctgcca
     3301 cgcttgatcc ggacgacgcc gcgcaggacg accgggaaga gcaggatcga aacaccgacg
     3361 ccaagggcaa tcggccacgc gtcgatccag cggaggaaat agagcgagcc gagaagccaa
     3421 ctgaggacgc cgatcgagac ggcgaccccg gcccatacca gccagagccg gttccgatcc
     3481 tggctgtcgc cgggcgaagg cgcgcgctcg ccggcattgg ccgcaatcaa ggccatgagg
     3541 gcccaaaagc cgagcggccc gccgctcaga atgtcggtga ggaggccgag gagaagagcg
     3601 agatagacgg ggaaggccgt gtcgggaagc gtccgccagc aaaacaccat cgccatcagg
     3661 agcatcggca ggatgaaagt cgcgccggcc ggaagccccc agggaatcgc ggtcgcgagc
     3721 gccgcggcaa acactgacaa agccggcaaa tacgcgctca tcgctgggtt tgggccacgc
     3781 tttcactggc gggcgtaccg ggcgttcccg cctgcttgat cgcgccagtc atctcaggct
     3841 gttcgaccga cccggttggg accggtgccg gaggtttgga gtcatcggac gatgcagaga
     3901 gtgctcggtt cagagaagcg gttggcgggt cggtacgcgt caaggcgggc gcgtcgaaga
     3961 acagcacgct caccacgtcg agcgcgctca gatccgcatg aggctcgatt ctgtaagcgc
     4021 cgggagaccc cgtcaccacg ccgacacgca agcctcgcgg cagcacgccg tcactgcccg
     4081 aggtgaacac ctcgtcgccg ggatggagca gggcaccctc gggaaggaaa tcgagcctga
     4141 gcgcggcgct gttgtcgcct gaggcgatcg cgcggacgcc ggcatgtccg accagcacgg
     4201 ggacccggct gctgaggtcg ttgaggagta ggatgcgggt gaccgaggcc ccggcctcga
     4261 ccgtccgccc gacaagaccg tcgccgttga tgacggcgta gccgacgcgc accccgttat
     4321 cgcgtccaag attgatcagc gcgctctgga gaaacggacc gcgcgcatcg gcgatcacgt
     4381 tgccggtggt gaagtgcaag gcgggctcgt ccacggcgtt cagcagggcg cggagttgcg
     4441 cgacattgcg ctcggcgagc ttggcgcgcc actcccactg cttgagcttc tcgttttcta
     4501 ctttcagccg gtcgatctcg ctgaacgtgc cgacatagga ccccacctgt gacgccgcgt
     4561 ggcgcgccat catgaccggg gtcgatgtgg cttcaagcag gggcgcggtc atatcgacga
     4621 cgctatcgcg cgcttcggtg agcgccgcgt gcccgatccg gctcaagacc agaagaacga
     4681 tgcagaggaa gatgagcgca aaattaagca attcacccgg agcccgccag gatcggcgag
     4741 gcctcggctt gaacggagaa tggtccccgc gcatttcttc atctttcctc gaaggtcacg
     4801 cgccgcccgc gtccttcgtc aatccggggc ttgcgcagcc tgcaagccct tcacccgcta
     4861 tcgccgtctc atggcttgac caaaaagtca gacatggtgt cgagctgctc caaagcctta
     4921 cccgttccgc gcaccacaca gcgcaaggga tcctctgcga tcttgaactt gacgccggtc
     4981 tcctgcgaga acctggcatc gagcctgtcg agcagcgcgc cgccgcccgt caggtaaatg
     5041 ccggaatcgc aaatatcagc ggtgagctcg ggcggcacgt cggcaagcgc ctggcgtatg
     5101 ccgcccacga tctgttggat cggaaggttc aaggcctgcg ggatgtcgtc gggcgtgagc
     5161 gcgatgtcgg ccgggaagcc gcgatggagg tcacgcccct tgagatggat cggcatgcgt
     5221 tcgccgttgg ccttggcgat ggcgcagccc gattccttct tgatggtctc ggcgctggtg
     5281 gcgccgatca gcaaattgta tttgaagcgg acatagttga cgatcgcttc gtcgaaagcg
     5341 ttgccggcgc tgcgcaccga gcgcgaatag agcacgctgc ccatggatag cacggcgatg
     5401 tcggtggtgc cgccgccgat atccacgatc atcgagccgc gtgcctcggt gatgggaaga
     5461 ccggcgccga tcgcggcagc gaccggctcc tcgatgagat agacgcgcct cgcgccagcc
     5521 gaaagtcccg cttcgtgcac cgcgcggcgc tcgaccggag tcgcgctcgc cgggacacag
     5581 atcatgatgc gcggactgct gaacagcggg cgcttgagca cgcggcggat gaaatgcttg
     5641 atcatctcct ccgcggcgtt gaagtcggca atcacgccgt cgcgcagagg ctgaatggtt
     5701 tcgatcttgg acggggtgcg gccaagcatg ctcttggcct cgtagccgac ggcgatgacc
     5761 ttgcgatcac cgtcgcgctg ctgcgtgacg gcgacaacag aaggttcgtc gagcacgatc
     5821 ccccgccccg gcacgtaaac gagagtgttt gccgtgccaa ggtcgatggc catgtccgcc
     5881 gagacaaagc ctaagagccg cgagaacata cgctcgccct atctggctcc ccgcaacgag
     5941 ccaacgagcc acggaagtgt gtgatgggca ggcggcgaca cctttggcga ctgattcttg
     6001 ccgtcacccg accggcggta tgttgcggca aatcggccct gggggcaatg agtcggagac
     6061 ccgatttgaa aaaaaacaat ttgcttgcat cgctccttcg cgggcgccgc ccggaaggtc
     6121 gtggaggagg cagtccggcg cggccgatgc ggcggtagcg ttaacgccgc ccctgaacgc
     6181 cgggacccaa acggaccgct agcctttgat tctagggcga aaataccggt cgggcaggtc
     6241 ggtcccgggc tgcgccctcg tcatgggcac gcgccctcct ccggctgcct cgccgcggcc
     6301 ggcggggtca ggacgcgagc atcgactcgg gcttccgccg ggcctcgcgc gccagcaact
     6361 tattcagggc actgacataa gcccgggccg aagcgaccag cgtgtcggtg tcggacccgc
     6421 gtcccgtcac ggtcttgccg tcggcttcga gccgtacgct gacctcggcc tgggcgtcgg
     6481 ttccttccgt caccgcgctc acctggtaga gcgggagcct cgcctcgtgc ggcacgatcg
     6541 ccttgatcgc cctgaaggtg gcatcgacgg ggccgtcgcc ggtctgttca cacgtctgct
     6601 gcttgccgtc gatatcaagg gtgatggtcg ccttctgggg accgccggtg cccgcgatca
     6661 cggtgagcgc cacgaccttg atccggtcat gcgcgctcgc gatctcctga tcgacaaggg
     6721 cttcgagatc ctcgtcatag acgtgcttct tgcggtcggc gagcgccttg aagcgcacga
     6781 aggcgtcctc gaaggcgttg tcgccaagct cgtagccgag ctccttcagc ttctcgcgga
     6841 aggcgtgacg ccccgaatgc ttgcccatga caagcgaaga ctccctcagg cctacgctct
     6901 ccggcgtcat gatctcgaac gtctcggcat gcttgagcac gccgtcttga tgaatgccgc
     6961 tttcatgggc gaaggcgttc cgcccgacga tggccttgtt gtactggacc gggaaagcgg
     7021 tcacgcggga gacgagcctc gaggcgcgcg tcagcatggt ggtgtcgatg ccggtataga
     7081 acggcatagc gtcgccccgc gtcctgatgg ccatgacgat ttcttccagc gcggcgttgc
     7141 ccgcgcgctc gccaagaccg ttgatcgcgc attcgacttg gcgcgcgccg gcgccgacgc
     7201 ccgcgagaga gttggcgacg gcaagaccga gatcgttatg gcaatgggtc gagaacacga
     7261 tcttctcggc gccggggacg cggatgagga tcatgccgat gagcgcggcg aactcatcgg
     7321 gcgtggcata gccgaccgtg tcggggatat tgacggtggt ggcgccggcc ttgatcgcgg
     7381 cctccacgca gcggcagagg aagtcatgct cggtgcgggt cgcgtccatc gccgaccatt
     7441 cgacgtcgtc ggtatagctc cgcgcgcggg tgaccgaggc ggtgacccgc tcgagcacct
     7501 ccttctcgtc gatgctcatc tgatatttgc gatggagcgg cgaggtgccg ataaaggtgt
     7561 gaatgcggcc gcgcgcggcg ggcttgatcg cttcggcgca gcggtcgatg tccttgaatt
     7621 gcgcccgcgc aagcccgcag atcaccgcct tcttggcgcg gcgggcgatc tcctggacgc
     7681 tgttgaaatc gccttccgag gcgatgggga agccggcctc gatgatatcg acgcccatct
     7741 cgtcaaggac ctcggccacc tcgagcttct cctcgaaggt catggtggcg ccggggcact
     7801 gctccccgtc gcgcaaggtc gtgtcgaaga tcaccacgcg gtcgcgctcg gagcggcctt
     7861 tggccttggc gctgtcttta cgggactcca tctcgttcgt ccttaagctt cgggccccag
     7921 tctagcatga ctggcaagcc gactgaatct ggtggatttt tcttgccatc ccctgaacgc
     7981 ctggcgcggc ctcaagccca gcgccgtcgg gtgttcaggg gcgactaagg aggagcccgg
     8041 agctcagcgg gccagcggaa tcggacgcgg aagcgtcaat tttcgcctcg ggttgcccgc
     8101 gaacgtcttg catcgatcat ccttcgcttc ggccgggaac atagtcggct gcgccgctcc
     8161 gagcaaggcc atccccagcg ccgtcaggtg ggcgggaccg ggggcgccgg atgcgcatca
     8221 cggaccgcaa caactcgctt ccgcctgacc agagaatctg gattccgagg caaaatagga
     8281 tgaaggccga gagccgcacg gcgatattgg tgccgcccgg ccccaacagg cgctggacgc
     8341 ggtccgcgta cgcgaaacac acaaaaatgg cggcggcaat cggcctcact ggtcgaactc
     8401 gtataggagc cccgagagag tccgagactg atgacgacgg cgatggtccc gggccccgtc
     8461 gtcaatggca gcgtcagggg atagaaggcc tggtcgaggg gattggccgg ccggcggccg
     8521 tccgtcgaag cttccagttc cttctggctg ccctcggtga ggagcttcca ccccgcgacg
     8581 gcgacgatga taccgcccgc gacgcgaagg accggtatcg aaatgccgta gaagcgtagg
     8641 atcagcgcgc cgggcgacaa tgagccgaca aggacgaaga aggcgtttcg cgcaacgcgc
     8701 caggcaataa gccggcgaac ctcatgggtc acgttgcccg tcatgccaag gaagacaagc
     8761 gccgaccctg gcggattgac gaccggaaag agcgccgcga gttcgagaag gaagatgttc
     8821 agtacggagt ccatggcgcg gtgggacaaa cgatcgttgc atgatcatgc gcagatgttt
     8881 ccgtgacaat cctcacataa aatcgccgcg gcggcgttta ggcgagtccg caggcggggc
     8941 gagatcgccg tcgggcagac tgacaaagat cagaacgccg agcaccgtca cgtagaagcg
     9001 gaaggccgcg tcctggccgt tccagtcttt cgactgccac atgtcgaacc attcgcccgc
     9061 caccaccatg aaacccaaga accagacgag gaacgcgagc aggcatccgg cgatgacgag
     9121 ggtcttgcct tggttgaacg cggcgccgga ggcgctgcgc aagcgaacga gctggatgcc
     9181 gcccgccaga aacaggagac cgg
//
LOCUS       DASUQP010000226        13049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1065107, whole genome shotgun sequence.
ACCESSION   DASUQP010000226 DASUQP010000000
VERSION     DASUQP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065107"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..991
                     /locus_tag="VFY74_10375"
                     /pseudo
     CDS             230..991
                     /locus_tag="VFY74_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            1038..1538
                     /locus_tag="VFY74_10380"
     CDS             1038..1538
                     /locus_tag="VFY74_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5932137.1"
                     /translation="MPIASQKINPCLWFDTEAEDAARFYCSIFKNSKVGTISRYCSEG
                     KEIHGKDAGSVMAVEFELDGQRFAALNGGPQFKFSEAISFQVHCDDQKEVDYFWNKLS
                     DGGEEGPCGWLKDRYGLSWQIVPTVLFKMLTDPDRAKSQRVTKAFLQMKKFDIPALER
                     AYAGKN"
     gene            1560..2090
                     /locus_tag="VFY74_10385"
     CDS             1560..2090
                     /locus_tag="VFY74_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5932138.1"
                     /translation="MTGTKTKPDATKAPAFEFFIRRAFNAPRARVWKAWTEEKSLGQW
                     WGPKDFEVVSAKLDLKPGGIFHYLLRSPEGQEMWGKLIFREIVPQERLVFIVSFSDAE
                     GGLSRHPLHEGWPLAILSTVTFADAGPGRTEITVRWVAHDATDAEHEVFEEGKDSMTA
                     GWTGTFDRLDAYLART"
     gene            2109..2519
                     /locus_tag="VFY74_10390"
     CDS             2109..2519
                     /locus_tag="VFY74_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5932139.1"
                     /translation="MHLNPYLTFDGNCEEAFRTYEKILGGKIIAMMAHEGTPAEDHVP
                     AEWRKKILHARLVFGDNVLMASDAPPGRFAKMQGFSVTLVVKEPAEAECLFNALAEGA
                     TITMPLGETFWAAKFGMLTDRFGTPWMINCEKPA"
     gene            2545..3024
                     /locus_tag="VFY74_10395"
     CDS             2545..3024
                     /locus_tag="VFY74_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEX5932140.1"
                     /translation="MDNPMSGLTLAAALGAGLVAGIFFAFSAFIMAALGRLRPENGIS
                     AMQSINVVVLNPVFFFVFFGTALAALILAIAALAGSSPSRSLYPLAGALLYLAGTIFV
                     TMAFNVPLNNRLASASANSAEGASVWNTYLSAWTAWNHVRTVAAMAACASFIMVLSE"
     gene            3050..3409
                     /locus_tag="VFY74_10400"
     CDS             3050..3409
                     /locus_tag="VFY74_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX5932141.1"
                     /translation="MPYVDGFVIPVPKKKLVAYRSMARKAGKVWREYGALDYKECVAD
                     DVKMGKRTSFPRSVKKKPNETVVFSYIVYKSRAHRDKINAKVMKDKRIAGMMDMSAMP
                     FDVKRMIYGGFKVIVDA"
     gene            3610..4035
                     /locus_tag="VFY74_10405"
     CDS             3610..4035
                     /locus_tag="VFY74_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5932142.1"
                     /translation="MKARIKWVEDRTFIGESGTGHKIVLGTAFGPEGRTPGPSPMELV
                     LIGLGACSVFDVVHILAKGREPIEDASVELDAERAQEDPKVFTRVHMHFAVKGRGLAP
                     EKVARAIALSIEKYCSASAMIAKTATITHDFEVVDTAVT"
     gene            complement(5198..5273)
                     /locus_tag="VFY74_10410"
     tRNA            complement(5198..5273)
                     /locus_tag="VFY74_10410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5238..5240),aa:Val,seq:gac)
     gene            complement(5432..5821)
                     /locus_tag="VFY74_10415"
     CDS             complement(5432..5821)
                     /locus_tag="VFY74_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5932143.1"
                     /translation="MEAKDPRARSVQRILLAEDDEDMRRFLVKALEKAGYDVIAFGNG
                     LEAYERLKEEPFTLLLTDIVMPEMDGIELARKASELDPGLKIMFITGFAAVALNPDNH
                     APKDAKILSKPFHLRDLVDQVERMLAA"
     gene            6052..6951
                     /locus_tag="VFY74_10420"
     CDS             6052..6951
                     /locus_tag="VFY74_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5932144.1"
                     /translation="MSGSEVEAIESELSPPFTVARPEPLKIPLVFNSPHSGRVYPKAF
                     LAASRLDSLALRRSEDAYVEELFGFVTALGAPLLHAHFPRAYLDVNREPYELDPVLFR
                     DGLPHYANTQSVRVIGGLGTIARIVSESDEIYREPLSVGAALERISRLYTPYHDTLSS
                     LLADAHGAFGCALLIDCHSMPSTPITEQGGVRPDFVLGDRFGTSCNGELTRLAANQLK
                     ALGHAVTLNKPYAGGYITEHYGRPHKSQHVLQIEINRALYMDETSFEKSGGFERLRDD
                     LEAMVAALITGIPSLAVPRAAAE"
     gene            7227..8024
                     /gene="hisN"
                     /locus_tag="VFY74_10425"
     CDS             7227..8024
                     /gene="hisN"
                     /locus_tag="VFY74_10425"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX5932145.1"
                     /translation="MPASDNPEFQSLLATAHALADRAGSVILPHFRTEQAVDHKGGDL
                     FDPVTAADREAETAIRARIAEAYPAHGILGEEFGLRDGEAEYCWVIDPIDGTRSFILG
                     LPLWGTLIGLTRGGVPLLGVMDQPFTGERFWSGETESFFRHDGATRTIRTRPCADLRQ
                     ALIATTSTDYFTSAEQQRFAALTHAVRLRRFGGDCYNYCLLAMGHIDLVAEAGLQPFD
                     ILPLIPIVERAGGVVSSWDGGDPRPGGRILAAGDPRLHAAAVKMLSG"
     gene            complement(8021..8980)
                     /locus_tag="VFY74_10430"
     CDS             complement(8021..8980)
                     /locus_tag="VFY74_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5932146.1"
                     /translation="MDLIGTPKNPIPLGASTGYIQPRKGLRLRFASWQSVLRERRGTV
                     CILPGRAEFIEKYFEVVGELRRRGFAVAILDWRGQGGSTRLTRNPLKGHIKDFAEYED
                     DLNHFMKSVVLPDCPAPYYALGHSMAAMVLFRASTKRGCWFTRIIMTAPMVRIAELPF
                     SHKMCERLADGLTLLGFGKRAVPGDANRHRQDPPFEGNRLTSDRERLLRNVSVLEAAP
                     QLAVQSPTIGWLKAALDTIEIVDSEKFAPRIRVPVLMLTAGDDQIVSSKAVEALALRL
                     RAGAQIVLRGSRHEILQERDSIREQFWAAFDAFVRDAALSKAS"
     gene            9304..9774
                     /locus_tag="VFY74_10435"
     CDS             9304..9774
                     /locus_tag="VFY74_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX5932147.1"
                     /translation="MRHFDLTPLYRSTIGFDRLGSLLDTLASFEGDAPSYPPYNIERV
                     GENEYRISMAVAGFGESDLAIEVKENTLSIRGEKRVEQEDTTFLHRGIASRSFERRFQ
                     LADHVLVKGATMENGLLHVDLVRELPEAMKPRTIPIATKTDKAEKPKVIDSKAA"
     gene            complement(9975..10748)
                     /locus_tag="VFY74_10440"
     CDS             complement(9975..10748)
                     /locus_tag="VFY74_10440"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX5932148.1"
                     /translation="MTGFAEIQSAFARAVLDAETLVPAGLARKADRVPARRFRVYRNN
                     VYSGLIDVLAGRFPVVARLVGEEFFRAMARLYVEREPPASAVLIRYGASFPNFITDFP
                     PAASVPYLADMAALEWAWHSAYHAADSVPLPLHALAAATDRAADAVLALHPSLSIVRS
                     LYPIVSIYELNVQAGDVPPTQVSGKEDALVARPRLQVEIRRLPEGGAPFISALKNGSP
                     IGEAAAVASREAPEFDLETNLAGLIASGAIIGLRASAGG"
     gene            complement(10752..11623)
                     /locus_tag="VFY74_10445"
                     /pseudo
     CDS             complement(10752..11623)
                     /locus_tag="VFY74_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329925.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
     gene            complement(11675..11995)
                     /locus_tag="VFY74_10450"
     CDS             complement(11675..11995)
                     /locus_tag="VFY74_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEX5932149.1"
                     /translation="MKDQVEGQARSLSGVAIGCAFAAALGLVATGPVAGAADDKEKCY
                     GVAKAGENDCAAGPGTTCSGTSKIDYQGNSWKLVPKGTCLTMELPDGRKGSLEALTRD
                     LPPA"
     gene            12430..>13049
                     /locus_tag="VFY74_10455"
     CDS             12430..>13049
                     /locus_tag="VFY74_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX5932150.1"
                     /translation="MASREQKLRSSPEAWPSPPAAQGLYDPKHEHDACGVGFVADLKG
                     RPSRKIIEDSLQVLENLTHRGAAGADPLAGDGAGILVQIPHDFLRAVCAPLNIELPEP
                     GHYGVGQMFMPRNQAQRIYCESVIERVIEAEGLTLIGWRDVPCDNSCLSASVIATEPV
                     HRQVFIGRGPGIEDETAFERRLFLLRKVISNTVMGETGGREIGFYTV"
BASE COUNT         2517 a         4155 c         4078 g         2299 t
ORIGIN      
        1 cgcgctcgga atctaggttg gccgttctac cgaacacgac acacattaca ctgatggaag
       61 aaggcaatgc catcatcccc atggtacacg atttcctcga cgccaaatcg cagaaacaat
      121 aaggcccgga catccggcca acgattccgc cgtgagcttc aacgcctctg cgacggggaa
      181 ccataagcga tcccagcgcg gcgcgcagac acttgttagg aggtcgaaca tgcaaaagca
      241 caacgttgta gcccacgaag aatggcttat cgcgcgcaag gcgcacctca agaacgagaa
      301 ggcgctcacc cggatgcgcg atctcgtcgc ggcggagcgg cggacgcttc cctggacgaa
      361 gatcgagaag cgatgatacg tcttcgattc gccggacgga aagaagacgc tcgccgatct
      421 cttcggcagg aacgatcagc tcgtcatcta tcacttcatg ttcggccccg actgggacgc
      481 aggctgcccg agctgctcgc tcgtggccga ccgcgccgag ggcgccctcg tccatctcga
      541 gaatcacggc gtgagctatg tccgcgttgc gcgggcgccg ctcgcaaaga ttgaggccta
      601 caggaagcgc atgggctgga cgggcaaatg ggtgtcgtct tgcggctccg acttcaattt
      661 cgacttccac gtctccttca cgccggacgc gctcgcaagc gggaagatcg aatacaacta
      721 tgcgaccatc accgatccaa aataccacca cgacgagctg ccgggccata gcgtgttctt
      781 caaggacacg aacggcgatg tgttccacac ctactccagc tacgcgcgcg gcaacgagga
      841 ggtgatcggc gccttcatct atctcgacat gacgccgagg ggccgcaacg aaaaacagat
      901 catggattgg gtgcggcggc acgacgagta cgaagacgcc ccgaaatccg attcctgctg
      961 cggggaggcc aagcagacga gcgccgcata gccgtcgcgt cacgttcagg aacaatcgcc
     1021 aagtcttgga ggaggccatg ccgatcgctt cccagaagat caatccatgc ttgtggttcg
     1081 ataccgaagc cgaggacgcg gcgcggttct actgctcgat cttcaagaac tcgaaggtgg
     1141 gcacgatcag ccgctattgc agcgagggaa aagagatcca cggcaaggac gcgggctcgg
     1201 tcatggcggt cgagttcgag ctcgacggcc agagatttgc cgcgctgaac ggcggcccgc
     1261 agttcaagtt cagcgaggcc atctcgttcc aggtccattg cgacgaccaa aaggaggtgg
     1321 actatttctg gaacaagctc tccgacggcg gcgaggaagg accctgcggc tggctgaagg
     1381 acagatacgg cctctcctgg cagatcgtgc ccacggtcct gttcaagatg ctgacggacc
     1441 ccgaccgagc caagtcgcag cgggtcacga aggcctttct acaaatgaag aagttcgaca
     1501 tccccgcgct cgaacgcgcc tatgcgggca agaactgacg aaacgagagg agacacgacg
     1561 tgaccggcac caaaactaaa ccggacgcaa cgaaggcgcc ggcgttcgaa ttcttcatca
     1621 ggcgcgcctt caatgcgccg cgtgcgcggg tctggaaagc ttggaccgag gagaaaagcc
     1681 tcggccaatg gtgggggcca aaggacttcg aagtcgtctc ggcgaagctc gatctgaagc
     1741 ccggcggaat cttccactac ctgttgcgct cgcccgaggg ccaggagatg tggggcaaac
     1801 tgatcttccg cgagatcgtg ccacaggaac ggctcgtttt tatcgtctcc ttctccgatg
     1861 cggagggcgg cttgagccgt cacccgctgc acgaaggctg gccgctcgcc attctctcca
     1921 cggtgacctt cgccgacgcc gggccgggca ggaccgagat tactgttcgc tgggtggcgc
     1981 acgacgcaac ggacgccgag cacgaggtat tcgaagaggg caaggactcg atgacggccg
     2041 gctggacggg gaccttcgat cggctcgacg cctatcttgc aagaacctga caccgcaagg
     2101 aacgcgccat gcatctcaat ccctatctga cgttcgacgg caattgcgaa gaggccttcc
     2161 ggacctatga gaagatcctt ggcggcaaga tcatcgccat gatggcgcat gagggcaccc
     2221 cggccgagga tcatgtgccg gcggaatggc gcaagaagat cctgcacgcg cggctcgtct
     2281 tcggcgacaa tgtgctgatg gcctcagacg cgccgcccgg ccggttcgcg aagatgcaag
     2341 gattctccgt gacgctggtc gtgaaggagc cggccgaggc ggagtgcctc ttcaacgcgc
     2401 tggccgaagg cgcgacgatc accatgccgc tcggcgagac gttctgggcc gcaaagttcg
     2461 gcatgctgac cgaccgcttc ggcacgccgt ggatgatcaa ttgcgagaag ccggcgtgac
     2521 cgaaccggaa gctcctaagc ggcaatggac aacccgatgt ccggcctgac gctcgcggca
     2581 gcgcttggcg ccggcctcgt cgctggaatc ttcttcgcct tctcggcctt catcatggcc
     2641 gcgctcggac gcctccgccc cgagaacggc atctccgcca tgcaatccat caatgtcgtg
     2701 gtgctgaacc cggtgttctt cttcgtgttc ttcgggaccg ccctcgcggc gctcatattg
     2761 gcgatcgcgg cgcttgccgg atcgtcgccg tcacggtcgc tttatccgct tgcgggcgcc
     2821 ctgctctatc tcgccggaac aattttcgtg acgatggcct tcaacgtgcc cttgaacaac
     2881 aggcttgctt ccgcgagcgc aaacagcgcc gagggcgcga gcgtctggaa cacatatctt
     2941 tcggcctgga cggcctggaa tcatgtgcgg acagtcgcgg cgatggccgc gtgcgcatcg
     3001 ttcatcatgg tcttgagcga atagaaaccg agccctaagg agaacgcgta tgccctatgt
     3061 cgatggattc gtgatccccg tcccgaagaa gaagcttgtc gcctatcgca gcatggcgcg
     3121 caaggccggc aaggtctggc gcgaatacgg cgcgctcgat tacaaggaat gcgtcgccga
     3181 cgacgtgaag atgggcaaac ggacgtcgtt tccgcgcagc gtcaagaaga agccgaacga
     3241 gacggtggtg ttctcttaca tcgtctacaa gtcgcgcgcc catcgcgaca agatcaacgc
     3301 caaggtgatg aaggacaagc ggatcgccgg aatgatggac atgagcgcca tgcccttcga
     3361 cgtgaagcgc atgatctatg gcggcttcaa ggtgatcgtg gacgcctagc cgccccgcgc
     3421 gacgcttcgg ttgcggcgct gccggacaac gccggaccaa cgtccgatca aaattcaggt
     3481 atagatctcc aaccctcggg acggactgtt tcgctccgca ctacggtctg gtcgcgcggg
     3541 gcgcccctag ttttcgggcg ttcggcagcc gtcgcgttac cgtgttgaac caatgcaggc
     3601 tggcttttga tgaaggcgcg catcaaatgg gttgaggacc gcacgttcat cggcgagtcc
     3661 gggacggggc acaagatcgt tctggggacg gccttcgggc cagaggggcg cacgccggga
     3721 ccgagcccca tggagcttgt gctgatcggc ctgggcgcct gttccgtgtt cgatgtggtt
     3781 cacattcttg cgaagggccg cgagccgatc gaggatgcgt ccgtcgagct tgacgccgag
     3841 cgcgcgcaag aggatccgaa ggtctttacc cgggttcaca tgcatttcgc ggtgaagggg
     3901 cgagggctgg caccggagaa ggtcgcgcgc gccatcgcgc tgtcgatcga gaaatattgc
     3961 tcggcgtcgg ccatgatcgc caagacggcc accatcaccc acgacttcga ggtcgtcgat
     4021 accgccgtaa catgaggcat cgcacggaag atggtgggcg caggacaatc tcgggaccct
     4081 ccaagattta gccaccacaa ggggcctcga ctgcaatacc ccgcgtccga aaagaagggg
     4141 gatgaccttg gaaccctgag ggtgcggatt ggttgtgcgt gcgtcccaga cgggagatgg
     4201 aggcgtatca tgcctgatag accccttcct acagacagcc gcgaagccgc gcccgtgata
     4261 attacggagt agacagccgc gaggcccgcg cccgcttcag tgataattac gtcgtcatcg
     4321 gtatcgtggc tgtatgtgtg cttatggccg ttgccctact tggcgcggga atgttggata
     4381 ccggcgaaaa gcgcaccacg cagccaagca cccaagcacc ggtcaccacg cagtcggaca
     4441 ctcaagcccc ggccatcacg cagccgagct ctcaagaacc ggcaacaggt gaaaataaaa
     4501 caccgtaact ggggcgcggc gtggggaggc caatacggcc tcagacacgg gctcaactgc
     4561 gggttcccgc ctacccttgc ggacgctcgc ctccaggcat gccttcagac cggctgcttg
     4621 tgcgtcttga gcaggtccga aaagcctcct cggccggttc ctgaaatgca gatccggcga
     4681 tggcttcctg gcgagagttc cgccgaaatc tcggagacga actcgcgcga ccatgttcgc
     4741 aaacgaatag gagcgcccgt gacgcacgga cgccccttcg gctagcctga aacagccaca
     4801 tgggaggcaa ctccaggccc gttatctcaa cccgcgagat gctttctggt tcacctgccg
     4861 aagcgagcgc cgctagcgat cgtgggcaat aaacttattc agaagctact ggcggtttgc
     4921 cctgattctc ctcgccggga gcgatagccg cgtctggatt tactccgctt tgcccggtgc
     4981 cagggggagc ttggctgcag gggtggcgtg ctggacggta cgccctcgac tgatcccgac
     5041 ggaaggacgg agaggcatgc gcgtcacgcc ggtcctccat cggaacggaa ccggcacgcc
     5101 accccctccc ccgactccca agccgctccc aagaaggccc ggtgtaatcc gtcgccgcgt
     5161 ccgcgtggta aataaatcat tgatttgctt gccggaatgg tgggcgcgac tgggatcgaa
     5221 ccagtgaccc ctgcgatgtc aacgcagtgc tcataccgct gagctacgcg cccgctcaac
     5281 cgaagaaccg gtcaggcttc caacgtcagg cttctatatc ggttgcggcg ccgtcaaggc
     5341 aacccgcgcc gccccttctc gaggcaaccc gcgccgccct cgaccaaggc atctcgcgcc
     5401 gcccgcagag gccacccgcg ccgcctcaag atcacgccgc gagcatccgc tccacctggt
     5461 cgaccaggtc gcgcaagtgg aacggcttgg acaggatttt cgcgtccttc ggcgcatgat
     5521 tgtcggggtt cagcgccacc gccgcaaagc ccgtgatgaa catgatcttg agcccgggat
     5581 cgagttcgct cgccttgcgc gcaagctcga tgccgtccat ctccggcatc acgatatcgg
     5641 tgaggagaag cgtgaagggc tcctccttca gccgctcgta ggcttcgagc ccgttgccga
     5701 acgctatgac gtcgtagcca gccttctcga gcgccttcac caggaagcgg cgcatgtcct
     5761 cgtcgtcctc ggcaaggaga atacgctgca cgctgcgcgc ccgtgggtct ttcgcctcca
     5821 tccctcgtcc ccctgactct cgccttacgg cgtacacaaa cttggtaaat gacttggtaa
     5881 gcaacgctgg ccggtcccga gcgtacacag cccttggccc ttccaacccc aaatcatcgc
     5941 gtctatttgc tctggacaca aggcagaaca cgccttgcca ggttcaactc tgattggcac
     6001 agtttacaag cgattcccgg gtcgcaaggg cgaacgtcca aggaacggcc gatgagcgga
     6061 agcgaagttg aagcgatcga gagcgagctt tcgccgccct tcacggtggc gcggcccgag
     6121 ccactgaaaa tcccgctcgt cttcaactca ccccatagcg gccgcgtcta cccgaaggcc
     6181 tttcttgccg cctccaggct cgattcgctg gcgcttcggc gctcggagga cgcctatgtc
     6241 gaggagctgt tcggcttcgt caccgcgctc ggcgcgcccc tcctccacgc ccatttcccg
     6301 cgcgcctatc tcgacgtcaa ccgcgagccc tacgagctcg atccggtgct gttccgcgac
     6361 gggctccccc attacgccaa tacgcaatcg gtccgggtga tcggcggtct cggcacgatt
     6421 gcgagaatcg tttcggaatc cgacgagatc taccgcgagc cgctgtccgt cggcgccgcg
     6481 ctcgagcgca tcagccgtct ctacacgccc taccacgaca cgctgagcag cttgctggcg
     6541 gacgcgcacg gcgcgttcgg ttgcgccttg ctgatcgact gccactcgat gccctcgacc
     6601 ccgatcaccg agcagggagg cgtgcgccct gatttcgtgc tcggcgaccg tttcggcacc
     6661 tcctgcaatg gagagctgac ccggcttgcc gcgaaccagc tgaaggcgct tggccatgcc
     6721 gtgaccctca acaagcccta tgccgggggt tacatcaccg agcattatgg gcggccccac
     6781 aagtcccagc acgtgttgca gatcgagatc aatcgcgccc tctatatgga cgagacgagc
     6841 ttcgaaaagt ccggggggtt cgagcggctg cgcgacgacc tcgaagccat ggtggccgcg
     6901 ctcatcaccg gtatcccgag cctcgccgtg ccccgggcgg cggccgaata aagccggcac
     6961 gacatgaaaa aggggccgtt cgcgtgaacg aacggcccaa gtctagggag gaaacgccca
     7021 aggagggcag cggcacggcc tggagagcca taccgcgttg caacaatatg tcattgcggc
     7081 gcacaaaaat caagtggatg gcccgttaag cgcatttctg aaacagtgat gcatatcggc
     7141 cacgagggca tgcgtcttgc cttgggcctc caaaaacaaa cctggaagcc ttgactctca
     7201 aggcatagcg gcctcaaccc tctcccgtgc ccgcatctga caaccccgaa tttcagtccc
     7261 tgctcgccac cgcgcatgcg ctcgccgatc gcgccggttc ggtcatattg ccgcatttcc
     7321 ggacagagca ggccgttgac cataaaggcg gcgatctctt cgatcccgtc acggcagcgg
     7381 atcgcgaggc ggagactgcg atccgggcga ggatcgccga ggcctacccc gcgcacggca
     7441 tcctcggcga ggagttcggc ctgcgcgacg gcgaggcgga atattgctgg gtcatcgatc
     7501 cgatcgacgg cacgcgctcg ttcatcctcg gtctcccgct ctggggcacg ctgattggat
     7561 tgacccgcgg cggggtgcct ttgctcgggg tgatggatca gcccttcacc ggcgaacgct
     7621 tctggagcgg cgagaccgag tccttcttcc gccatgacgg cgcgaccagg acgatccgca
     7681 cgcggccttg cgccgatctc cgtcaggcgc tgattgccac gacgagcacc gattacttca
     7741 cgtcggcgga gcagcaacgc ttcgctgcgc tcacccacgc cgtccgcctc cggcgtttcg
     7801 gcggcgactg ctacaattac tgcctgctgg cgatggggca tatcgacctc gtggccgagg
     7861 caggcctcca gccgttcgac attcttcccc tgatcccgat cgtcgaacgt gcgggtggcg
     7921 tggtctccag ttgggatggc ggcgatccgc ggccgggcgg gcgcatcctt gccgccggcg
     7981 acccgcgcct tcatgcggca gcggtcaaga tgttgtcggg ctagctcgcc ttgctgaggg
     8041 cggcatcgcg gacgaaggcg tcgaatgccg cccagaactg ttcgcgaata ctgtcccgtt
     8101 cctggagaat ctcgtggcgc gagcctctga ggacgatctg cgcgcctgcc ctcaggcgaa
     8161 gggcgagcgc ctccaccgcc ttgctcgata cgatctgatc gtcgccggcg gtgagcatga
     8221 gcacaggcac gcggatgcgc ggcgcaaatt tttcgctgtc gacgatctcg atcgtgtcaa
     8281 gcgcagcctt gagccagccg atggtcggcg actggacggc gagttgcggc gcggcctcga
     8341 gcaccgacac attgcggaga aggcgctcgc ggtccgaggt gaggcggttg ccttcgaacg
     8401 gcggatcctg acggtgcctg tttgcgtcgc cgggcaccgc acgcttgccg aagccgagca
     8461 gggtcagccc atcggcaagc cgctcgcaca ttttgtgcga aaacggcagc tccgcgatcc
     8521 tcaccatggg cgcagtcatg atgatgcggg tgaaccagca accgcgcttg gtcgacgccc
     8581 tgaacaggac catggcggcc atggagtgtc cgagcgcgta atagggcgca gggcagtccg
     8641 gcagcacgac agatttcata aagtggttga ggtcgtcctc gtactcggcg aaatctttga
     8701 tatggccctt gagcgggttc ctggtgagcc gggttgatcc cccctgcccg cgccaatcga
     8761 ggatcgccac ggcaaagccg cggcgcctga gctcgcccac cacctcgaaa tatttctcaa
     8821 tgaactcggc gcggccggga aggatgcaga ccgtgccgcg ccgctcccgc agcaccgact
     8881 gccagctggc gaagcgcaag cgcaggccct tgcgcggctg gatataaccg gtcgaggcgc
     8941 cgagcgggat cgggttcttg ggtgtgccta taagatccat tgaagcgagc gtaacccctc
     9001 aagcgcgagc gcctcggccc gagcgcttgg cgcatccgtc acgacgcgag cgccccttat
     9061 agcagcgaag cgcgcgtgcg ccacggtctt cgggcccctc atttagcccg cgacccagcg
     9121 atccgcctcc gccgccgaga agtgcgattc cgcctttata ttcagcaccc tacacctctt
     9181 gacgcgaccg cctgccgcag ccatatcacg ggcgcgcgaa ggcccacacg gctttgcgcg
     9241 atccgcccgg cttgaacgac ctaagcgggc acaatccata tcgcttatca aggaggatgt
     9301 gtcatgaggc actttgatct taccccgctc tatcgctcca ccatcggctt cgaccggctc
     9361 ggctcgttgc tcgatacgct tgccagtttc gagggcgacg ccccgagcta cccgccctac
     9421 aacatcgagc gcgtcggcga gaacgagtat cgcatcagca tggccgttgc cggcttcggc
     9481 gagagcgacc tcgcaatcga ggtgaaggag aacacccttt ccattcgcgg cgagaagcgc
     9541 gtcgagcagg aggacacgac cttcctccat cgcggcatcg cgtcgcgaag cttcgagcgc
     9601 aggttccagc tcgccgacca tgttctggtc aagggcgcaa ccatggagaa cggcttgctg
     9661 cacgttgatc tcgtgcgcga gctgcctgag gccatgaagc cgcgcaccat cccgatcgcc
     9721 accaagaccg ataaggccga gaagccgaag gtcatcgaca gcaaggcggc gtaacggctg
     9781 actggcgtag aaccgactga aacaagaagg gcgcctcctc gcatgagggg acgcccttct
     9841 ctgcaagcag gtgcttagga gagtgacctg cggggccccc tgcacgcaag actaatcgtc
     9901 gggggcagtg acggagccta tcagtcacaa cttgcgcgag ccttaacgcg ccggccggag
     9961 ggcgcaaggt ctcgttaacc gccggcgctc gcgcgaaggc cgatgatcgc cccgctcgca
    10021 atcagcccgg cgagattagt ctcgaggtcg aattccggcg cttcacggga ggcgacagcc
    10081 gcggcctcac cgatcgggct cccgtttttg agcgcggaga tgaagggagc accgccttca
    10141 ggcaggcggc gaatctccac ttgaagtcgc ggccttgcaa cgagcgcatc ctctttgccc
    10201 gaaacttgcg ttggcggcac gtcacccgcc tgtacgttca gttcgtagat cgacacgatc
    10261 ggataaagcg agcgcacgat gctgagcgat gggtggagcg cgagcacggc atcggcggcg
    10321 cgatcggtcg cggctgcgag cgcgtgaagc ggcagcggca cggaatcggc cgcgtgatag
    10381 gcgctgtgcc atgcccattc gagcgcggcc atgtcggcga gatagggcac cgacgccgcg
    10441 ggtggaaagt ccgtaatgaa attcgggaaa ctcgcgccgt agcggatcag aaccgcggag
    10501 gcgggcggtt cgcgctcgac atagagccgg gccatggcgc ggaagaattc ctcgccgaca
    10561 agccgggcca ccaccggaaa ccgcccggcg agtacgtcga tcaggccgga atagacattg
    10621 ttgcgataga cgcggaagcg ccgggccggc acgcggtcgg ctttgcgcgc gagcccggca
    10681 ggaaccaaag tctcggcatc gagcacggcg cgcgcgaacg cgctttgaat ttctgcaaag
    10741 ccggtcatgg ctcaggccgt gcagcgattt gagcggcgat cggcgatgac gcggtccgcc
    10801 cgtttcgcct cgtcgaacag agccgcccat gccggaacgt cattgtccca ctcgatcagc
    10861 gtgggtgttg ggcctaggcg cgcgagcgca tgcgcgtaaa gcgtccatac ggcagcggcg
    10921 acttcacgcc catggtcatc gatcagaaga acggaaccgt cgtcgtcgct gtcggcggca
    10981 tggccgccaa gatggatctc gccgacatgc tcgacgggaa aggcgtcgat gaaggtgccc
    11041 gcgtcgaaac cgtgattgac ggattggacg aacacattgt tgacgtcgag cagaagcccg
    11101 cagccggtgc tgcgcgccac ctcgcgcagg aattcgacct cgtccatcgc tgcatcggcg
    11161 aaggccacgt aggtcgaagg attctcaagc agcatgcgcc gtccgagcgt ctcttgcacc
    11221 tcgtcgatat gctcgcacac acgcgcgagt cctccgtgta aggcaagggc aaaaggtcgt
    11281 tgagatagac gccgtcatgg ctcgaccagg cgagatgctc ggagaagagc gcgggctcat
    11341 agcgcgcgat gagagccttg agacgcaaaa gatgctcgcg gtcgagcggc gcgctgccgc
    11401 cgatcgaaag cccaacgcca tgcagcgaca ccggatagcg ctcgcggata gcgcccagat
    11461 aacggtgggg cggcccgccc tcccccatgt agttctcggc atggatctcg aaccagccga
    11521 tgtcgggtgt ggtatcgagg atatcgcggt aatgctcggg cttgagcccg acaccggcac
    11581 ggggcgggag gggtttcagc ctttcggatg gggtcgcggt catcttgcgg ttcagatccg
    11641 aaggaaaaac cccgccacgc cgttacaggc cgcgtcaggc cggcggaagg tcgcgcgtaa
    11701 gcgcctccag gcttcccttc cgtccatcgg gaagctccat ggtcaggcac gttcccttcg
    11761 gcacgagctt ccaggaattg ccctggtaat cgatcttcga ggtcccggaa caggtcgtgc
    11821 cgggccctgc ggcgcagtcg ttctcgcccg cctttgcaac gccgtagcat ttctccttgt
    11881 catcggcggc gccggcgacc gggcccgtcg ccacgagacc gagcgcagcg gcaaaggcac
    11941 agcctatggc gacgcccgac agactcctcg cttgaccctc aacttgatcc ttcatggaat
    12001 gctccttgtt ggtggaaatg ctcccggcac cggaactccc ggcaggcgct tgtggttgcg
    12061 ttagggagta agtctgcgcg ggcgcgatca cgtcaaatca cagctccgcc agattgcggg
    12121 tcagacgggc cgggcctccg ggcttctgaa cggtgaatcc ttcttgtgtg cgcgttttaa
    12181 atctgtcatt ttgagggcaa ataggacagg cttgctgacc tttgtcgcgg aggcggcgca
    12241 aggcaccaag gaattggcgc gaagagtcac tttggtcctc gcccgcatcg ccaatggagg
    12301 cggccagccg ccgaggcgcc tgcttcgttt tcagcgagtt acgtttcagc tgctacagca
    12361 cgggcgccga agaagcgttt tggatttgcg cgcgggcggc gtgagccgta gccgaggttt
    12421 caagtgacga tggcatcgcg tgagcagaaa ttgcgttcct cgcctgaggc gtggccttcg
    12481 cctccggccg cgcaaggact ctacgatccg aagcacgagc acgatgcctg cggcgtcggc
    12541 ttcgtcgccg atctcaaggg acggccgagc cgcaagatca tcgaggacag cttgcaggtt
    12601 ctggagaatc tgacccaccg cggcgccgcc ggggccgacc cgcttgccgg cgacggcgcc
    12661 ggaatcctcg tgcagatccc gcatgatttt ctccgtgcgg tttgtgcgcc gctcaatatc
    12721 gagctgcccg aacccggcca ttacggcgtc ggccaaatgt tcatgccgcg caatcaggcc
    12781 cagcgcatct attgcgagtc cgtcatcgag cgcgtgatcg aagcggaagg gctcaccctc
    12841 atcggctggc gcgacgttcc ttgcgacaat tcgtgccttt ccgcgtcggt gatcgcaacc
    12901 gagcctgtgc accggcaggt gttcatcggc cgtggtcccg gcatcgagga tgagaccgct
    12961 ttcgagcgca ggctcttcct gctgaggaag gtgatctcga acaccgtcat gggcgagacg
    13021 gggggaaggg agatcggctt ctacaccgt
//
LOCUS       DASUQP010000227         7933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_656683,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000227 DASUQP010000000
VERSION     DASUQP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656683"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFY74_10460"
     CDS             <1..511
                     /locus_tag="VFY74_10460"
                     /inference="COORDINATES: protein motif:HMM:NF021148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEX5932151.1"
                     /translation="AGLWVNGPGKVYVSVPGFYAALSTRPFRAIAPDETLKALGIGFG
                     NLDFGRATQGDPEEEKFRSAVIRLKQKQRLFQEDDDGVDFVGRSLFKGTVALPVNVPT
                     GRYTADIYLFRDGEVISRNQSTLAVNKAGFEGVVYRLAYADPFVYGVLAVVLAVLAGL
                     AGWYAFRRE"
     gene            complement(515..1240)
                     /gene="pdeM"
                     /locus_tag="VFY74_10465"
     CDS             complement(515..1240)
                     /gene="pdeM"
                     /locus_tag="VFY74_10465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEX5932152.1"
                     /translation="MLRAKHDLMPETAPTLRSHLAVAGHAMRPLPAGALYWEDERTLL
                     VADLHLEKGAAFAALGMLLPPYDTRATLDRLTRVIDAVQPLRVVALGDSFHRSECAAR
                     LPAEDRDTLLALQRGREWFWICGNHDPHLPASIGGTVCATLTICGLTLRHEPSPAASA
                     REIAGHLHPVARIARRGTLIRRRCFATDGNRLVMPAFGAYAGGLNLRDEAFALLFHRH
                     RLEAWMMGRANVYPVIGSLLLPD"
     gene            complement(1221..3770)
                     /locus_tag="VFY74_10470"
     CDS             complement(1221..3770)
                     /locus_tag="VFY74_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEX5932153.1"
                     /translation="MSASESLFAEAPRALPEPFASWFKARGWQPHAHQLALLAQAGAG
                     RDTLLIAPTGGGKTLAGFLPSLTDLARRPARPAPETAIHTLYVSPLKALTVDIARNLA
                     TPVAEMDLPIKLETRTGDTPPSRRARQRLLPPHILLTTPEQIALMLSHRSAADLFGAL
                     NFVILDELHALASSKRGDLLALDLARLRTLSPGLTAIGLSATVARPYELAAYLMPHRG
                     KAATRLAEIVVAGEGARADISILRSDAALPWAGHSARYALTEIYAAIEAHRMSLVFVN
                     TRSQAEMLFNGLWRHNDANLPIALHHGSLDVAQRRKVEAAMVQGKLRAVVCTSTLDLG
                     IDWGDVDLVINVGAPKGASRLAQRIGRANHRLDEPSKALLVPANRFEVIECQAALEAA
                     AEGAQDTPPLRPGALDVLAQHVLGTACAGPFDEDRLYREVTSAAPYQGLLREGFDQVV
                     QFVATGGYALANYERYAKLKRTPEGQYRLAHPRIAQQYRLNVGTIVEAPMLRVRLARG
                     RGGALKPSHMLAAGRVLGEVEEYFIEQLMPGDTFIFAGEVLRFEGLRETDAIVTRTHA
                     TEPKIPAYEGGKFPLSTYLAERVRGLIADGQHWRRLPDQVSDWLRLQAEFSVVPGPQE
                     ILVETFPRGARHYLVCYPFEGRLAHQSLGMLLTRQLERARAAPLGFVANEYALAVWGL
                     NDIGAMVRDGRLDLAQLFDEDMLGDDLDAWLAESSLMKRSFRVCAIISGLIERRHPGQ
                     EKTGRQMTASSDLIYDVLRQHEPGHILLKAAFADAATGLIDVYRLGDFLRRIKGRIRH
                     IGLGRVSPLAVPVMLEIGRESVSGEASEILLRETVEELVAEALGEHAARQT"
     gene            3887..4189
                     /locus_tag="VFY74_10475"
     CDS             3887..4189
                     /locus_tag="VFY74_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HEX5932154.1"
                     /translation="MERFFGGNPALVLLRLALLSLIVGVMLAALGFSPYEIIDSIARL
                     VERIYDLGFEAVEKAIRYFLLGAVIVFPIWLVVRLFKFAGRAERDLVDPTSPKREA"
     gene            complement(4431..5501)
                     /locus_tag="VFY74_10480"
     CDS             complement(4431..5501)
                     /locus_tag="VFY74_10480"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX5932155.1"
                     /translation="MNALFGLGQALLFALDPERAHDLAVKSLELGLYPRSDAPDDKRL
                     RQHLFGLDFPNPIGMAAGFDKSARVARELLGMGFGFVEVGTLTPRAQSGNPSPRIFRS
                     GQDRAIINRLGFNNDGQEAALARLKGGSGGIVGVNLGAGRDSTDRIGDYAEGIAHMAP
                     VANYFTVNISSPNTPGLRDLQAPEALALLLKRVQDARAASVGKPPLLVKLAPDLVDED
                     LPEIVRVILDHGADGIVVSNTTLSREGLGDVSFAKEAGGLSGRPLFARATRMLARVYR
                     LTEGRLPLIGVGGIDSGASALAKIEAGASLVQLYTGLVFEGPSLIGRMKQALIAAIEH
                     AGAKDLGPLIGVRAEEWANSPE"
     gene            complement(5498..5635)
                     /locus_tag="VFY74_10485"
     CDS             complement(5498..5635)
                     /locus_tag="VFY74_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932156.1"
                     /translation="MASPVYKIFTEPHLYAPLDLAAVLWTERPSLGEDGVHSLPEGVF
                     A"
     gene            complement(5616..7226)
                     /locus_tag="VFY74_10490"
     CDS             complement(5616..7226)
                     /locus_tag="VFY74_10490"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX5932157.1"
                     /translation="MEAETPARTAAHESKAWPFEEARKVLSRLERVQKPTGAVIFETG
                     YGPSGLPHIGTFGEVARTTMVRHAFRVLTEDKIPTRLICFSDDMDGLRKVPDNIPNKE
                     MVAEHLGKPLTQVPDPFGEHASFGHHNNARLRAFLDTFGFDYEFLSATSCYTSGRFDA
                     VLRKVLEHYDAVRDVILPTLGPDRRATYSPFLPISPTTGQVLQVPIIDRDVAAGVVLY
                     KDPGTGKLTQVPVTGGRCKLQWKADWAMRWAALGVDYEMAGKDLIDSVRLSSQICRVL
                     GAQPPEGFIYELFLDEKGEKISKSRGNGLTIEEWLTYASPESLSLYMYQSPRKAKRLY
                     FDVIPRAVDEYVGHLAAYRKEEPAARLMNAVWHIHQGAPPEAELPISFALLLNLASAS
                     NSEDREVLWGFIRRYAPEATPEAHPLLDQLVTYAIRYFHDFVKPAKRYRAPNEKERAA
                     LLDLDARLAKMPPGLPAEEIQAQVYAVGKEHGFEPLRDWFGALYAVLLGQDQGPRFGS
                     FVELYGIAETRSLIAKALKGELDGKSRL"
     gene            7407..7820
                     /locus_tag="VFY74_10495"
     CDS             7407..7820
                     /locus_tag="VFY74_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEX5932158.1"
                     /translation="MTEPLNHQAALIYVMVTMSAVDRAITDAELARIGEVVSEMPVFA
                     DFNKDNLIKTAEDCGEILSADGGLDHVLLLIRANLPQKLRETAYAVALEVAAADLDVR
                     PEETRFLEMLRDTLDLDKLTTAAIERGIRARNLVL"
BASE COUNT         1365 a         2556 c         2638 g         1374 t
ORIGIN      
        1 tgccgggctc tgggtcaacg ggccgggcaa ggtctatgtg tcggttccgg gcttctacgc
       61 cgcgctgtcg acgcggccct tcagggcaat cgcccccgac gaaaccttga aagcgctcgg
      121 gatcgggttc ggcaatctcg atttcggccg ggcaacgcag ggcgatcccg aggaagagaa
      181 gtttcgctcc gccgtgatcc gcttgaagca gaaacagagg ctgttccagg aggatgacga
      241 cggcgtggac ttcgtcggca ggagcctgtt caaggggacg gtcgccctcc cggtcaacgt
      301 tcccaccggc cgctataccg ccgatatcta tctgttccgc gacggcgagg tcatcagccg
      361 aaatcagagc acgctcgcgg tcaacaaggc cggattcgag ggcgtggttt atcggctcgc
      421 ctatgccgac cccttcgttt acggcgtgct tgcggtcgtg ctcgcggtgc tggccgggct
      481 tgccggctgg tacgctttcc gccgcgaata ggctttagtc cggcaggagc aggctcccga
      541 tcacgggata gacgttggcg cggcccatca tccacgcctc gagccgatgg cggtggaaga
      601 gaagcgcgaa ggcctcgtcg cgtaagttga gccctccggc ataggcgccg aaggccggca
      661 tgaccaggcg gttgccgtcg gtggcgaagc agcgccggcg gatcaaggta cctcgtctgg
      721 cgatgcgcgc cacggggtgg aggtggcccg cgatctcccg ggcagacgcc gcaggcgacg
      781 gctcgtgccg gagcgtcagc ccgcaaatgg tgagcgtggc gcaaacggtg ccgccgatgc
      841 tcgcgggaag atgcggatcg tgattgccgc agatccagaa ccattcgcgg ccccgctgga
      901 gcgcgagcag agtgtcgcgg tcttccgccg gaagacgcgc ggcgcactcg ctgcgatgga
      961 agctgtcgcc gagcgcgacc acgcgcaagg gctgaaccgc gtcgatcacc cttgtcagcc
     1021 gatcgagcgt cgcgcgcgtg tcgtaaggcg gaagcagcat tccgagcgcg gcgaaagccg
     1081 cgcccttctc cagatgcagg tcggcaacga gaagcgttcg ctcgtcttcc cagtagaggg
     1141 ctccggcggg aagcggccgc atggcgtgac cggcgacggc gagatgcgag cggagcgtcg
     1201 gcgccgtctc cggcatgaga tcatgtttgg cgcgcagcat gttcccccag tgcctcggcg
     1261 acaagttcct cgaccgtctc tcgcaacaag atctcgctcg cttcgccgga gacgctttcg
     1321 cgtccgatct cgagcatcac cggaacggcg agcggggaaa cgcgccccaa gccaatatgc
     1381 ctgattcgtc ccttgattcg cctcaagaaa tcgccgaggc gatagacgtc gatcagcccg
     1441 gtcgcggcat cggcgaaggc ggctttcagc aaaatgtggc cgggctcgtg ctggcgcagc
     1501 acgtcgtaga tcagatcgga ggacgccgtc atctgccgcc cggtcttctc ctgcccggga
     1561 tgccgccgct cgatcaatcc cgagatgatg gcgcagaccc tgaaggagcg cttcatcagg
     1621 ctcgactcgg cgagccaggc gtcgagatcg tcgccgagca tgtcctcgtc gaagagttgc
     1681 gcaagatcga ggcgtccgtc acgcaccatc gcgccgatat cgtttaaacc ccagacggca
     1741 agcgcatatt cgttggcgac gaagccgagc ggcgctgcgc gcgcccgttc gagctgccgc
     1801 gtgagcagca tgccgaggct ctgatgggcg agcctgccct cgaacgggta gcagacgaga
     1861 taatgtcgcg cgccgcgtgg aaaggtctcg accaggatct cctgtggtcc gggaacgacc
     1921 gagaactcgg cttgcaggcg gagccaatcc gagacctgat cgggaaggcg ccgccagtgt
     1981 tggccgtcgg cgatcaggcc ccgcacgcgc tcggcgagat aggtcgagag cgggaatttg
     2041 ccgccttcat aggccggtat tttcggctct gtcgcgtgcg tgcgggtgac gatggcgtcg
     2101 gtctcgcgca agccctcgaa gcgcagcact tcgccggcga agatgaaggt atcgccgggc
     2161 atgagttgct cgatgaaata ttcctcgacc tcgccgagca cgcggcctgc ggcaagcata
     2221 tgcgagggct tgagcgcccc gcccctgccc cgcgcgagcc gcacgcgcag catcggcgcc
     2281 tcgacgatgg tcccgacatt gagccggtat tgttgcgcaa tgcggggatg ggcgaggcgg
     2341 tactgcccct ctggcgttcg tttcagcttg gcgtagcgct cgtagttcgc gagcgcgtag
     2401 ccgccggtcg ccacgaattg caccacctgg tcgaaaccct cgcgcaagag accctggtag
     2461 ggcgcagcac tggtcacctc gcgatagaga cgatcttcat cgaacggacc ggcgcaggcc
     2521 gtgccgagca cgtgctgggc gagcacgtcg agcgcgcccg gccgcaacgg gggcgtatcc
     2581 tgcgccccct ccgccgccgc ctcgagcgcc gcctggcact cgatcacctc gaagcggttg
     2641 gccggcacca gcagtgcctt gctcggctcg tcgagccggt gattggccct gccgatgcgc
     2701 tgcgcaagcc ggctcgcacc tttcggcgcg ccgacattga tgacgagatc gacatcgccc
     2761 cagtcgatgc cgagatcgag cgtcgaggtg caaacaacgg cgcgaagctt gccttgaacc
     2821 atcgccgctt cgaccttccg ccgctgcgcc acgtcgagcg agccgtgatg gagcgcgatc
     2881 ggaagattcg cgtcgttatg gcgccacagc ccgttgaaca gcatctcggc ctgcgagcgc
     2941 gtgttgacga acacgagcga catacggtgg gcctcgatcg ctgcgtagat ctcggttagc
     3001 gcatagcgcg ccgagtgccc ggcccagggc agcgcggcat cggaacgcag tatcgagatg
     3061 tcggcccgcg cgccctcgcc cgccaccacg atctcggcca gccgcgtcgc cgccttcccc
     3121 cgatgcggca taagataggc cgcaagctcg taaggccgcg cgacggtggc ggaaaggccg
     3181 atggcggtga gaccgggcga cagcgtcctg agccgcgcga gatcgagcgc caagaggtcg
     3241 ccgcgcttgc tcgaggcgag agcgtgcagc tcgtcgagga tgacgaaatt gagcgcgccg
     3301 aacagatcgg ccgcgcttct gtgcgacagc atgagcgcga tctgctccgg ggtggtgagc
     3361 aagatgtggg gcgggagcaa gcgctggcgc gcgcggcgcg agggtggcgt gtctcccgtg
     3421 cgggtttcga gcttgattgg cagatccatc tcggccacgg gcgtggcaag gttccgcgcg
     3481 atatcgacgg tgagcgcctt gagcggcgag acgtagaggg tgtggatcgc cgtttccggc
     3541 gctggccgcg ccggccggcg cgcgaggtcc gtcagggaag gcaggaagcc cgccagcgtc
     3601 ttgccgccgc cggtcggcgc gatcaggagc gtgtcgcggc ccgccccggc ctgcgcgagc
     3661 agcgccagtt gatgggcatg gggctgccag ccgcgcgcct tgaaccagga ggcgaagggc
     3721 tcgggcagcg cccttggcgc ctcggcgaac aggctctcgc ttgcgctcat ccacactgtc
     3781 ttgaccttct ccccccttcg caccaattct ggcgtaattc gagtcgaagc tcgtgggtct
     3841 tggctcctcg cgccgagcct cctcaagcgg aagggagagc gaagagatgg aacggttttt
     3901 tggcggcaat ccggccctcg ttctcctcag actggctttg ctctccctca ttgtgggcgt
     3961 gatgctggct gcgctgggat tcagccccta cgagatcatc gacagcatcg cgcggcttgt
     4021 cgagcgcatc tatgacctcg gcttcgaggc ggtcgagaag gcgatccgct acttcctgct
     4081 cggcgctgtg atcgtgttcc cgatatggct ggtggtgcgg ctgttcaaat tcgccggccg
     4141 ggccgaacgc gatctggtcg accccacctc gcccaagcgg gaagcctgag ccgctctaga
     4201 ggatcgccag cacacgctcg ggaggcctta gcgaaagccc tcgttcgtca tgcccggact
     4261 tgatccgggc acccattcgt gacctccgat ccaagccgct cgcgccaagt tgagggctca
     4321 atggaatgga ttgccggagc ctgtccccgg actcgatccg gggatcaagc ccggtaatga
     4381 cgaactcgtg gatgcccggc acttcgcccg ggcatgacgg gataactgta tcactctggc
     4441 gaattcgccc attcctcggc gcgaacaccg atcaatggtc ccagatcttt ggcgccggcg
     4501 tgctcaatgg ccgcgatcag cgcctgcttc atgcggccga tgagcgaggg tccttcgaac
     4561 acgaggccgg tatagagctg gacgagcgag gcgcctgcct cgatcttggc gagcgcgctc
     4621 gcgcccgaat cgatgccgcc gacgccgatc agcggcagcc tgccttcggt cagccggtaa
     4681 acccgcgcca gcatccgcgt ggcgcgggcg aagagcggtc gccccgaaag gccgccggcc
     4741 tccttggcga aggagacatc gccgagtccc tcccgcgaca gcgtggtgtt cgacaccacg
     4801 atcccatcgg ccccatgatc gaggatcact cgcacgatct ccggcaggtc ctcgtccaca
     4861 agatcgggcg caagcttgac caggagcggg ggcttgccga ccgaagccgc gcgtgcgtct
     4921 tgcacgcgct tgagcagcag agcgagcgcc tccggcgcct ggagatcgcg caaacccggc
     4981 gtgttgggcg atgagatgtt gacggtgaaa tagttcgcga cgggcgccat gtgcgcgatc
     5041 ccttcggcat agtcgccgat gcgatccgtg ctgtcgcgcc ccgccccgag attgacgccg
     5101 acgatcccgc ccgagccgcc cttcagccgg gcaagggccg cctcctgccc gtcattattg
     5161 aagccgagcc ggttgatgat ggcgcggtct tggcctgagc ggaagatgcg ggggcttggg
     5221 ttgccgcttt gcgcccgcgg cgtcagcgtg cccacctcga cgaaaccgaa tcccatgcct
     5281 agaagctcgc gcgcgacgcg agcgctttta tcgaagcccg cagccatgcc gatcgggttg
     5341 ggaaagtcga ggccgaacag atgctgcctg agccgcttgt cgtccggcgc atcagaacgc
     5401 gggtacagtc caagctcgag gcttttgacg gcgagatcgt gcgcccgctc cggatcgagc
     5461 gcgaacagga gcgcctgccc gagaccgaac agagcgttca cgcgaaaact ccttccggca
     5521 agctgtgaac gccgtcctca ccgagactag gtctttcggt ccacagcacg gcggcaagat
     5581 cgagcggcgc gtagagatgc ggctcggtga aaattttata gacgggactt gccatcgagc
     5641 tcgcccttga gcgccttggc gataagggag cgggtctccg cgatgccgta aagctcgacg
     5701 aaggagccga agcgcgggcc ttgatcttga ccgagcagca ccgcgtagag cgctccgaac
     5761 cagtcgcgca aaggctcgaa gccatgctcc ttgccgaccg cgtagacttg cgcctggatc
     5821 tcttcggcgg gcagccctgg cggcatcttc gcaagtcgcg cgtcgagatc gaggagcgcc
     5881 gcccgttcct tttcgttcgg cgcgcgatag cgcttcgccg gcttcacgaa atcgtggaag
     5941 tagcggatgg cataagtgac gagctggtcg agcagcggat gcgcttccgg agtcgcctcg
     6001 ggcgcatagc ggcggatgaa gccccacagc acttcgcgat cctcagagtt cgaggcggac
     6061 gcgaggttga gcagaagcgc gaagctgatg ggaagctcgg cctctggcgg cgcgccttga
     6121 tggatatgcc agaccgcgtt catcagccgc gccgcgggct cttctttccg gtaggcggcg
     6181 agatggccga catattcatc gaccgcgcgc gggatcacgt cgaaatagag ccgcttcgcc
     6241 ttgcgcgggc tctgatacat ataaagagac aggctctcgg ggctcgcgta ggtcagccac
     6301 tcctcgatgg tgagtccgtt gccgcgcgac ttggaaatct tctcgccctt ctcgtcgagg
     6361 aagagttcgt agatgaaacc ctccggaggc tgcgccccaa ggacgcggca gatctggctc
     6421 gagagcctga ccgagtcgat caggtccttt cccgccatct cgtaatcgac gccgagcgcg
     6481 gcccagcgca tggcccagtc ggccttccat tgcagcttgc atctgccgcc cgtcaccgga
     6541 acttgcgtga gcttgcccgt gccggggtcc ttgtacaaaa ccacgccggc ggcaacgtcg
     6601 cgatcgatga tggggacctg aagcacctgc ccggtggtcg gcgagatcgg caggaacgga
     6661 ctataggtgg cgcggcgatc agggccgagc gtcggcagga tcacgtctct caccgcatcg
     6721 taatgctcga gcaccttgcg cagcacggcg tcgaagcgtc cgctcgtgta gcacgacgtg
     6781 gcggagagga actcgtagtc gaacccgaac gtgtcgagga aggccctgag ccttgcattg
     6841 ttgtgatggc cgaagctcgc gtgttcgccg aacgggtccg gcacctgcgt cagcggcttg
     6901 ccgagatgct cggccaccat ctccttgttg gggatgttgt cgggcacctt cctcaacccg
     6961 tccatgtcgt cggagaagca gatcaggcgg gtcggaattt tgtcctcggt cagcacgcgg
     7021 aaagcgtggc gcaccatcgt ggtgcgggcg acctcgccga acgtgccgat atgcggcaag
     7081 cctgaaggcc cgtagcccgt ctcgaaaatg actgcgccag tcggcttctg cacgcgctcg
     7141 agccggctca agaccttgcg cgcttcctcg aacggccagg ccttgctctc atgcgctgcc
     7201 gtccgcgccg gggtttcggc ttccattctg gcggtccggg tcatgggcgg cgaaaagtgt
     7261 cgtgggttgt cgcgattctg ccgttcgacc aatttcacaa atttcccgcc cgcgccactc
     7321 tttccatgcc ggtgcgggct tgcccggtta gtcgcaattg cctagggtcg cgccccttcg
     7381 aggaacgcaa ccgggagaag ggcaccatga ccgagccgtt gaaccatcag gccgcgctca
     7441 tctatgtcat ggtcaccatg tcggcggtcg atcgcgccat caccgacgcc gagcttgcgc
     7501 gtatcggcga ggtagtctcc gagatgccgg tcttcgccga tttcaacaag gacaatctga
     7561 tcaaaacggc cgaggattgc ggtgaaatcc tgagtgccga tggcgggctc gatcatgtgc
     7621 tcctgctgat ccgcgccaac ctgccgcaaa agctgcgcga gaccgcttac gcggtcgctc
     7681 tcgaagtggc cgccgcagac ctcgatgtga gacccgagga gacccgcttc ctcgagatgc
     7741 tgcgcgatac gctcgacctc gacaagctca ccacggcggc gatcgagcgc ggcatcaggg
     7801 cgcgcaatct ggtgctgtga taacgcgcgc tcgtacgcgc cgcacgatgc gcgtcattcc
     7861 ggcgtacctt aagctaaacc gtcatgcccg ttgatccggg catccattcc gtgaccccag
     7921 ggccaagctg agg
//
LOCUS       DASUQP010000228         6002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_521188,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000228 DASUQP010000000
VERSION     DASUQP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521188"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /locus_tag="VFY74_10500"
     CDS             <1..2186
                     /locus_tag="VFY74_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5932159.1"
                     /translation="PLLVLAGAGTGKTRVLTTRIAHVLSLGRARPAQILAVTFTNKAA
                     REMKGRVGELIGGVVEGMPWLGTFHAIGVKILRRHAELVDLKPSFTVLDTDDQIRLLK
                     QLLEAENIDEKRWPARVLAGMIDGWKNRGLVPKRVPEGESFAFANGKARDLYALYQLR
                     LKELNAADFGDLVLENLRLFQEHPDVLEKYQQRFLFTLVDEYQDSNVAQYLWLRLLAQ
                     GNHNICCVGDDDQSIYGWRGAEVDNILRFETDFAGAKIIRLERNYRSTGHILGAASGL
                     IAHNKGRLGKTLRTDGAIGDKVEVEGCWDDEEEARVIGEDIEQLQQQGHSLSEIAILV
                     RASFQMRAFEDRFITLGLPYRVIGGPRFYERQEIRDATAYFEVTLNASNDLKFERIFN
                     TPRRGLGESSLKSVHQLARAQGVSLFQAARLIAETEELKAKPRKALQDLIAAFDRWRS
                     LIDTMRHTELAELILDESGYTGMWQADRSPQAQSKLENLKELIRFMEEFDTLAGFLEH
                     VSLVMDAETEEGADRVNLMTLHSAKGLEFDTVFLPGWDEGLLPHQRSLDETGRAGLEE
                     ERRLAHVGITRARKGAKITFAQNRRNHGTWQTALPSRFIDELPVEHIAVAEEKGLRGY
                     GPSRFDTAETLFASGFYETPGWQRAQRNRPRGSGAAAKRGPVTIEGTLVASSTAEGAG
                     LVIGMRVFHQKFGYGRVTQIDGNKLTVDFDKAGQKKVVDSFVEKA"
     gene            2608..3519
                     /locus_tag="VFY74_10505"
     CDS             2608..3519
                     /locus_tag="VFY74_10505"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5932160.1"
                     /translation="MQAATAGRVTHQARIEVPAEAAMRLAEALEEALDPSPVAVGLFE
                     HGEDRFEVFAHYDAAPHREALLRLIAEAAPLDGVGPLHIETLPPADWVSLSQGKRGKV
                     VAGRFLIHGSHDRVNVPRRLLAIEIDAGQAFGTAHHASTRGCLLALDDLFKRRRPRTV
                     IDLGTGTGILAIAAAKCLRRKLIASDSDPVAAATAAANARKNGVASLIGVVTANGFAH
                     PRLRQNKAELVLANLLERALYALAPQLARHVVPGGTAVLSGLTETQARAIAARSQAHG
                     FVMKKRIILEGWATIVIVRRSAAFVKD"
     gene            3554..5371
                     /locus_tag="VFY74_10510"
     CDS             3554..5371
                     /locus_tag="VFY74_10510"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX5932161.1"
                     /translation="MFQLFDEVSSPAASGERIKALQGELKSRKLKGFLVPHSDEHQNE
                     YLAPQFERLAWLTGFTGSAGSAVVLEKDAALFVDGRYKLQARAQTDPALFEVLQTPEA
                     KASSWIAEKVGKGAALGYDPSLHTVKEIERLTETLAKSGIKLVPVETNPIDAVWQDRP
                     KPLAAAVVPHGVEYAGRSAHEKIRDVQAVLAEEKADAVLLTLLDSIAWLFNIRGGDIR
                     HTPVAFAFALVPATGRPTLFIDPAKIGDNVKGEIRESADLAEPGELTGRLKTLGEARA
                     RVRLDPETAPIRFAQALKAEGAQIVEGQDPCIMPKAIKNATEIKGARTAHLRDGVAMV
                     RFLAWLDEVAVSGKIDEIAAAMKLEDCRRESGQLKDISFDTISASGPNGAIVHYRPTT
                     KGKRLLEAGSLYLIDSGAQYRESTTDVTRTVAIGTPTVEMRRHYGLVLKSHIAIAAAR
                     FPKGTRGQDLDPFARRPLWEAGLDFDHGTGHGVGSYLSVHEGPQRLSRLGTVELQPGM
                     ILSNEPGYYREGHYGIRLENLVMVTPLEKIPDGEREMMGFETLTLVPFDRRLIDPKQL
                     LPWERDWLNAYHARVRREIEPILLSDDRAWLRHATAPIG"
     gene            5407..5661
                     /locus_tag="VFY74_10515"
     CDS             5407..5661
                     /locus_tag="VFY74_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HEX5932162.1"
                     /translation="MPMEILVAGVAATLTTAAFLPQAWHIIRLKETRAISLYMYASFA
                     TGVAFWFAFGIMIANWPIIISNAITLVLALAIIAMKLRYG"
     gene            complement(5633..5914)
                     /locus_tag="VFY74_10520"
     CDS             complement(5633..5914)
                     /locus_tag="VFY74_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932163.1"
                     /translation="MKGVHHVHAWTLTSGKNVFTAHLRVDEPRNTATVLRDAHDLLRK
                     KYGFYFSTLQVETTCLDEDHARDLDISTLLLPVPARKKARLSRNEASSR"
BASE COUNT         1098 a         1973 c         1941 g          990 t
ORIGIN      
        1 gcccgcttct cgtcctcgcg ggcgccggca ccggcaagac ccgcgtgctg accacgcgca
       61 tcgcccatgt cttgagcctg ggacgcgcga ggcccgcgca aatcctcgcc gtcaccttca
      121 ccaacaaggc ggcgcgcgag atgaagggcc gcgtcggcga gctgatcggt ggcgtggtag
      181 agggcatgcc ctggctcggc accttccacg ccatcggggt caagatcctg cgccgtcatg
      241 ccgagctcgt cgatctcaag ccgtccttca ccgtgctcga taccgacgat cagatcaggc
      301 tgctcaagca gctgctcgag gccgagaaca tcgacgagaa gcgctggccc gcgcgcgtgc
      361 tcgcgggcat gatcgacggc tggaagaaca gagggctcgt gcccaagcgc gtgccggagg
      421 gcgaaagctt cgcctttgcc aacggcaagg cgcgcgacct ctacgcgctc tatcagctgc
      481 ggctgaagga actgaacgcc gccgatttcg gcgatctcgt gctcgagaat ttgcggctgt
      541 tccaggaaca tcccgacgtg ctcgaaaaat accagcagcg tttcctgttc acgctggtgg
      601 acgagtatca ggattcgaac gtcgcccaat atctgtggct gcggctgctc gcgcaaggca
      661 accacaacat ctgctgcgtc ggcgacgacg atcagtcgat ctacggatgg cgcggcgccg
      721 aggtggacaa catcctgagg ttcgagaccg acttcgccgg cgccaagatc atcaggctcg
      781 agcgcaacta ccgctcgacc ggtcacatcc tcggcgcggc ctcggggctg atcgctcaca
      841 acaagggacg tctcggcaag acgctccgca ccgatggcgc gatcggcgac aaagtcgagg
      901 tcgagggctg ctgggacgac gaggaggaag cgcgtgtcat cggcgaggat atcgagcagc
      961 tccagcagca ggggcattcg ttgagcgaga tcgcgatcct cgtccgcgcc tcgttccaga
     1021 tgcgcgcctt cgaggaccgc ttcatcaccc ttggcctccc ctatcgcgtc atcggcggac
     1081 cccgcttcta cgagcgccag gagatcaggg acgccaccgc ctatttcgaa gtcacgctca
     1141 atgcgtcgaa cgacctcaaa ttcgagcgga tcttcaacac gccgcggcgg ggtctcggtg
     1201 aatcgagtct caagtccgtg caccagcttg cgcgcgcgca aggggtatcg ctgttccagg
     1261 cggcacggct gattgccgag accgaggagc tgaaggccaa gccgcgcaag gcgctgcaag
     1321 acctgatcgc cgccttcgac cgctggcgct cgctgatcga cacgatgcgg cacaccgagc
     1381 tcgcggagct gatcctcgac gagtcgggct ataccgggat gtggcaggcc gaccgctcgc
     1441 cgcaggcgca gtcgaagctc gagaacttga aagagctgat ccgtttcatg gaggagttcg
     1501 acacgctcgc gggcttcctc gagcatgtga gcctcgtcat ggacgccgag accgaggaag
     1561 gcgccgaccg cgtcaacctg atgacgcttc attccgccaa ggggctcgaa ttcgacaccg
     1621 tgttccttcc tggctgggac gaagggctcc tgccccacca gcgcagcctc gacgaaacgg
     1681 gccgcgcggg cctcgaagag gagcggcggc tcgcgcatgt cggcattacg cgggcgcgca
     1741 aaggagccaa gatcaccttc gcccagaatc gccggaacca cgggacgtgg cagacggcgc
     1801 tcccctcccg cttcatcgac gagcttccgg tcgagcacat cgccgttgcc gaagaaaaag
     1861 gcctgcgcgg ctacgggccg agccgcttcg acacggcaga gactctgttc gcgagcggct
     1921 tctacgagac gccgggctgg cagcgcgcgc agcgaaaccg tccgcgcggg agcggggctg
     1981 cggcgaagcg aggacctgtg accatcgaag gcacgctcgt ggcctcgagc acggcggagg
     2041 gcgcgggact cgtcatcggc atgcgcgtgt tccaccagaa attcgggtac ggccgggtga
     2101 cacagatcga cggcaacaag ctcacggtcg atttcgacaa ggccgggcag aagaaggtgg
     2161 tcgacagctt cgtcgagaag gcgtgagggg cctgccgact cttgagtccc ctatcgccga
     2221 gccatccgct tcccagacac ccctgatgag aacaaaacgt gtacattggt cacttctcac
     2281 cgaagggagg aagccccatg cggagcgtga tcgcaagcgg tttggtcctg atttcattgg
     2341 gatttctgtt gtttccggca ccctctccgg ccgttgccca agagatttgc cggtgcaagg
     2401 ggtgtggctg caagggcggc tcgggctggc gcggccccga cgggttctgc gtttcgcaag
     2461 gcaagctcgc ggaggtctgc ggcacgcctg cggccgcgcc gtgcaagcag gaggccgcgc
     2521 cccgcgtctg cttcggcaag tcgcaatcat aggctttgcg gcggaccgat ttgatactgc
     2581 atggcgccgg ggcctaagct ccgcgccatg caggcagcga ccgcggggcg cgtgacccat
     2641 caggcgcgga tcgaggttcc ggccgaggcg gcgatgcgtc ttgccgaggc gctcgaagag
     2701 gcgctcgacc cctcccccgt cgcggtcggt ctgttcgagc acggcgaaga ccgcttcgag
     2761 gtctttgccc attacgacgc ggcgcctcac cgcgaagctc tgctcaggct gatcgccgag
     2821 gctgcccctt tggacggcgt tggcccgctt cacatcgaga cgctcccgcc ggccgattgg
     2881 gtgagcctgt cgcaaggcaa acgcggcaag gtcgtggccg ggcgcttcct catccatgga
     2941 agccacgacc gcgtaaacgt gccgcgccgt ctgctcgcca tcgagatcga cgcgggtcag
     3001 gccttcggca cggcgcatca cgcgagcacg cgcggctgtt tgctcgcgct cgacgatctc
     3061 ttcaagcgtc ggcgtccgcg cacggtcatc gatctcggca ccggcacggg catcctcgcc
     3121 atcgccgccg cgaagtgtct cagacgaaaa ctcatcgcga gcgacagcga cccggtcgcg
     3181 gctgcaaccg ccgccgcgaa tgcgcgcaag aacggtgtcg cttccctgat cggcgtggtt
     3241 acggcaaacg ggttcgccca tccgcggctg aggcaaaaca aggccgagct cgtgctcgcg
     3301 aacctgctcg aacgcgcgct ttacgcgctt gcgccacaat tggcgcgcca tgtcgtgccg
     3361 ggcggcaccg ccgtcctgtc cggcctcacg gaaacccagg cgcgcgccat cgcggcgcgc
     3421 agccaagccc atggcttcgt catgaaaaag cgaatcattc tcgaaggatg ggcgacgatc
     3481 gtcatcgtcc gccgcagcgc tgccttcgtc aaagattgat cgcagtcgcg ttttgcgctt
     3541 acaacagccg gccatgtttc agctcttcga cgaagtgagt tcccccgccg ccagcggcga
     3601 gcgcatcaag gccttgcagg gcgaattgaa gtcgcgaaag ctcaaaggct tcctcgttcc
     3661 ccatagcgac gagcatcaaa acgagtacct tgcgcctcaa ttcgagcgcc tcgcctggct
     3721 cacgggcttc accggatcgg ccggttccgc cgtcgtgctc gagaaggacg cggcgctgtt
     3781 cgtcgatggg cgctacaagc tgcaggcgcg cgctcagacc gacccggcgc tgttcgaggt
     3841 gctgcagacg ccggaggcca aggcgtcgag ctggatcgcc gagaaggtcg gcaagggtgc
     3901 cgcgctcggc tacgacccgt cgctccacac cgtcaaggaa atcgagcggc tcaccgagac
     3961 gctcgccaag tcgggcatca agctcgtccc ggtcgaaacc aatcccatcg atgccgtctg
     4021 gcaggaccgg ccgaagccgc tggcggcggc ggtcgtcccc cacggcgtcg aatatgccgg
     4081 acgcagcgcg catgagaaga tccgcgacgt gcaggccgtg ctcgcggagg agaaggccga
     4141 cgccgtgctt ctcaccttgc tcgactcgat cgcctggctg ttcaacatca gaggcggcga
     4201 catcaggcac acgccggtcg cgttcgcctt cgccctcgtg ccggcgacgg ggcggccgac
     4261 tctcttcatc gatcccgcca agatcggcga caacgtcaag ggagagatcc gcgaatctgc
     4321 cgaccttgcc gagcccggcg agctgacggg ccggctcaag accctcggtg aggcgcgcgc
     4381 gcgcgtgagg ctcgatccgg aaacggcacc gattcggttc gcgcaggcgc tgaaggccga
     4441 aggcgcgcag atcgtggagg gtcaagatcc gtgcatcatg ccgaaggcga tcaagaacgc
     4501 gacggagatc aagggcgcac gcacggcgca tctcagggac ggcgtcgcca tggtgcggtt
     4561 cctcgcctgg ctcgacgagg tcgccgtgtc cggcaagatc gacgagatcg ccgcggcgat
     4621 gaagctcgaa gattgccggc gcgagtcggg ccagctcaag gatatcagct tcgacacgat
     4681 ctcggcttca ggacccaacg gggccatcgt ccattaccgg ccgacgacca agggaaagcg
     4741 cctgctggaa gcgggctccc tctatctgat cgattctggc gcgcagtacc gcgagtcgac
     4801 caccgacgtg acccggacgg tcgccatcgg cacgccgacg gtggaaatgc gccggcatta
     4861 cgggctcgtg ctgaagtcgc atatcgccat cgccgcggca cggttcccca aaggcacgcg
     4921 cggccaggac ctcgaccctt tcgcccgcag gccgctctgg gaggccgggc tcgatttcga
     4981 ccacggcacc ggtcacggcg tcggcagcta tctctcggtg catgaaggac cgcagcgcct
     5041 gtcccggctc ggcaccgtcg agcttcagcc cggcatgatc ctctccaacg agccgggcta
     5101 ttatcgcgaa ggccattacg ggatcaggct ggaaaatctc gtgatggtga cgccgctcga
     5161 gaaaatcccg gacggcgaac gggagatgat gggatttgag acgctgacgc tcgttccgtt
     5221 cgaccgccgc ctcatcgatc caaaacagct cctgccgtgg gaacgcgact ggctcaacgc
     5281 ctaccacgcg cgcgtcaggc gcgagatcga gccgatcctt ctcagcgacg accgggcctg
     5341 gctccgccat gccacggcgc cgatcggctg aatttagccc gccgcaggct ccgagaaaca
     5401 acaaacatgc cgatggagat cctggttgcg ggcgttgccg cgacgttgac cacggcggcc
     5461 ttcctgcccc aggcgtggca catcatccgc ctcaaggaga cgcgggcgat ttcgctttac
     5521 atgtatgcgt ctttcgccac cggggtcgcg ttctggttcg cgttcggcat catgatcgcg
     5581 aactggccga tcatcatctc gaacgcaatc acgctcgtgc ttgcgctcgc cattatcgcg
     5641 atgaagcttc gttacggctg agcctggctt ttttccgcgc cggcacaggc agcagaagcg
     5701 tcgaaatatc gagatcacgc gcgtgatctt cgtcgagaca tgtggtctcg acctggaggg
     5761 tcgagaagta gaacccgtat ttcttcctga gcaagtcatg cgcatcgcga agaaccgtgg
     5821 ccgtattgcg cggctcatcg acgcggagat gggcggtgaa cacgttcttg ccggaggtca
     5881 gcgtccaggc gtggacatgg tgcacgcctt tcatgtgcgg gagctgtcca aggcccgggc
     5941 gattgcggag aggtcgatcc cctccggcac gccctccatg aggacgaaga gggaatcgcc
     6001 ga
//
LOCUS       DASUQP010000229         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_929863,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000229 DASUQP010000000
VERSION     DASUQP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929863"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFY74_10525"
     CDS             <1..196
                     /locus_tag="VFY74_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932164.1"
                     /translation="ALVLLAGFPIAVPLFVFFYLVAESREKLGVSLVLAAAAWGAFHL
                     LFERLLHFPFEAGLIREWFQ"
     gene            305..1207
                     /locus_tag="VFY74_10530"
     CDS             305..1207
                     /locus_tag="VFY74_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5932165.1"
                     /translation="MIGASGGSLEIAARIAARHLGKHLPGNPNVIVQNMTGAAHLVAT
                     NHVYNVAKPDGLTLLAANPNVGIAQLTKVEAARFDLRRFQWLGSTGADGVALSVRADL
                     PYKSWEDLKKSDRELVVGTTGPGSNAHDFPLLLKEFAGAKFKLVSGYPANADVLLAIE
                     RKEVDVWAAFATTIKLAADRGAVRPLVRGRVALKGWEHLPVDEDLATSSVGKAIMAIR
                     AAPLAIGRALAAPPATPADRVKILREAFAKAVQDPEFIAESQKARIEVGYIPPEEVLK
                     MFNALLEQPADVQKEMVKYIKFGS"
     gene            complement(1244..2341)
                     /locus_tag="VFY74_10535"
     CDS             complement(1244..2341)
                     /locus_tag="VFY74_10535"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEX5932166.1"
                     /translation="MTAIAESPSSIERLRLYYSRHEGRVAATFFAAGFVFDMVTVGRI
                     DSWVTIGQQALYLAVILAALVQMFVEQFSPPRERWYSTYRSPLVHFLLGALLNLYAIF
                     YFKSSSLLVSFGFLGVLISLLLANELKRIKALGLPVKFGLAALCFLSFAVCVVPIAFG
                     SIGVGVFVTSMLAGSVPLAVVGFTAKPKEAARRQILVPLGCVLVLFLTFYFFRLIPPV
                     PLSIPFIGVYHNVEKTEAGYRLSHQRSAWRFWHNGDQSFVARPGDRVYVYFRVSSPGR
                     FADELQMRWHWKDPARGWTQHDRIPIRIVGGREQGFRGYGFKSNYRAGDWKVHVETAD
                     GREIGRVYFSLELAPEELGPQGERTFTTEID"
     gene            complement(2338..>2888)
                     /locus_tag="VFY74_10540"
     CDS             complement(2338..>2888)
                     /locus_tag="VFY74_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5932167.1"
                     /translation="ALFAIDSFAGGFVVHSLLALWLFQRFDLSLAAAGAFFFWSGVLS
                     AFSFPVAAWIGSRIGLINTMVYTHIPSSLCLIAAAFAPSVQWALGLLLVRAALSQMDV
                     PTRSSYVMAVVTEAERPAAASVTAVPRSLASAISPALAGALFSAGYQAWPLLLCGGLK
                     ILYDLLLLVAFRTIKPPEEQRA"
BASE COUNT          581 a          899 c          966 g          442 t
ORIGIN      
        1 cgcgctcgtt ctcctcgctg ggtttcccat cgccgtcccg cttttcgtat tcttctatct
       61 cgtggcggaa agccgcgaga agctcggcgt gtcgcttgtt ctcgcggccg ccgcgtgggg
      121 cgctttccat ctgctgttcg agcggttgct gcactttccg ttcgaggccg gattgatccg
      181 ggaatggttc cagtaaagag gaggaggatt catgcaaaag agcatcgtcg ccctttgcct
      241 tgtcgtagcg agccctgcgg cctgggggca agcgccgttc tacgaaggca agaccgtcac
      301 ggtcgtgatc ggcgcctcgg gtgggagcct agagattgcc gctcgcatcg cggcgcgcca
      361 tctcggcaag catctgcccg gcaatccgaa cgtgatcgtg cagaacatga ccggcgccgc
      421 gcacctcgtc gcgacgaacc acgtgtacaa cgtggcgaag cccgacggtc tgacgcttct
      481 cgcggcgaat ccgaacgtcg gcatcgcgca gcttacgaaa gtggaggccg cgcgcttcga
      541 cctgcggcgc ttccagtggc tcggatcgac aggcgcggac ggcgtcgccc tttccgtgcg
      601 cgccgatctt ccctataaga gctgggaaga cctcaagaag tccgatcgcg agctcgtcgt
      661 cggcacgaca ggacccggat cgaacgcgca cgactttccg ctgcttctga aggagtttgc
      721 cggtgcgaag ttcaagctcg tgtccggcta tccggcgaat gccgacgtcc tgctcgccat
      781 cgagcgcaag gaagtggacg tctgggccgc gttcgcgacc acgatcaagc tcgccgccga
      841 ccgcggcgcg gtgcgaccgc tcgtgcgcgg ccgcgtcgca ctaaaaggat gggaacacct
      901 gccggtcgac gaagacctcg cgacgagctc cgtcggcaag gccatcatgg caatccgcgc
      961 cgcaccgctc gccatcggcc gtgcactcgc cgcgccgccc gccacgccgg ccgatcgcgt
     1021 gaagatcctc cgcgaggcgt tcgccaaagc cgtgcaggac ccggaattca tcgccgagtc
     1081 gcagaaggcg cgcatcgagg tgggctacat accgccggaa gaggtactga agatgttcaa
     1141 cgccctgctc gagcagccgg cggatgttca gaaagagatg gtgaagtaca tcaagttcgg
     1201 cagttgaagc aaattgggcc cccgccttcg cgggggcgac ggctcagtcg atctcggtgg
     1261 tgaaggtgcg ctcgccttgc ggcccgagtt cctccggcgc gagttccagg ctgaaataca
     1321 cgcggccgat ctcgcgcccg tcggcggtct cgacgtgcac cttccagtcg cccgcgcggt
     1381 aattcgactt gaatccgtag ccacgaaagc cctgctcgcg gccgccgacg atgcggatcg
     1441 ggatccggtc gtgctgcgtc cagccgcgcg ccggatcctt ccagtgccag cgcatctgga
     1501 gttcgtccgc gaagcgcccc ggcgaagaga cacggaaata cacatagacg cgatcgcccg
     1561 gtcgcgcgac gaagctctgg tcgccgttat gccagaaccg ccacgctgag cgctgatggc
     1621 tcagtcggta gccggcctcg gtcttctcca cgttgtggta gacgccgatg aaggggatgg
     1681 agagcggcac cggcggaatc agccggaaga aatagaacgt gaggaagagt acgagcacgc
     1741 agccgagcgg cacgaggatc tggcgccgcg ccgcctcttt gggcttggcg gtgaatccga
     1801 ccaccgcgag cgggacgctg ccggcaagca tggacgtgac gaacactccg acgccgatcg
     1861 aaccaaaggc gatcggtacg acgcagacgg cgaaggaaag gaagcagagc gccgccagtc
     1921 cgaacttgac gggaagaccg agtgccttga tgcgcttcag ctcgttggca aggaggaggg
     1981 agatcagcac accgagaaag ccgaaggaaa cgagaagcga cgagctcttg aaatagaaga
     2041 tcgcgtagag gttgaggagt gcgccgagga ggaagtgcac gagcggcgag cggtacgtcg
     2101 aataccagcg ctcgcgcggc gggctgaact gctcgacgaa catctgcacg agcgccgcaa
     2161 ggatcacggc caggtagagc gcctgctgcc cgatcgtcac ccacgaatcg atgcgaccga
     2221 ccgtgaccat gtcgaacacg aagccggcgg caaagaaggt ggctgcgaca cggccttcat
     2281 gacggctgta gtagagccgc agccgctcga tgctgcttgg cgattcggcg atcgccgtca
     2341 tgctcgttgc tcctccggcg gcttgatcgt ccggaacgcg acgagtagca ggaggtcata
     2401 gaggatcttg agcccgccgc agaggaggag cggccacgcc tggtaaccgg cggaaaagag
     2461 cgctccggcg agcgccgggc tgattgccga ggccaggctg cgcggcacgg cggtgacgct
     2521 cgccgcggcg gggcgctccg cctcggtcac cacggccatc acataggaag agcgcgtcgg
     2581 cacatccatc tgcgacaggg ctgcgcgtac tagcagaagg cccagggccc actggaccga
     2641 aggcgcgaaa gccgcggcga tgaggcagag gctcgatggg atgtgcgtgt acaccatcgt
     2701 attaatgagt ccgatgcggc ttcctatcca cgcggcaacc gggaaggaga aggccgaaag
     2761 caccccgctc cagaagaaaa aggcacccgc cgccgccagg gagagatcaa agcgttggaa
     2821 aagccagagc gcaaggagcg aatgcacaac gaaaccgccg gcgaaggaat caatggcgaa
     2881 aagcgcgg
//
LOCUS       DASUQP010000230         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1338900, whole genome shotgun sequence.
ACCESSION   DASUQP010000230 DASUQP010000000
VERSION     DASUQP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338900"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..361)
                     /locus_tag="VFY74_10545"
     tRNA            complement(287..361)
                     /locus_tag="VFY74_10545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Gly,seq:ccc)
     gene            704..1681
                     /locus_tag="VFY74_10550"
     CDS             704..1681
                     /locus_tag="VFY74_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5932168.1"
                     /translation="MAEAALRAHPPKFSDPDLTATGERRARVALKALRTLWINTGSLC
                     NITCRNCYIESSPENDRLAYITRAEAALYLDEIARDGWPVTEIGFTGGEPFMNPDFIA
                     MLGDALSRGFSALVLTNAMQPMLRPRVMAGLLALRDAHRDRLVMRVSLDHYSLELHDT
                     ERSEGSYAKTIEGIDWLAREGFKLALAGRTCWGESEADLRDGYAALIAARGWPVDAYD
                     PAALVLFPEMDARADMPEITEACWSILGKSPADVMCASSRMVVKRKGAAAPIVLPCTL
                     LPYDPAFEMGETLAEAARADGGMFAGGAVKLCHVHCAKFCVLGGGACSA"
     gene            complement(1904..3112)
                     /locus_tag="VFY74_10555"
     CDS             complement(1904..3112)
                     /locus_tag="VFY74_10555"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX5932169.1"
                     /translation="MSSASQRDRLFEALPEDRPAGAPSDAPRSAFARTGILPSQAIRA
                     MIADGEIAGAPEIETNQIQPASLDLRLGKVAFRIRASFLPGPDATVRDKLAELKFHEI
                     DLTRGAVLEAGCVYLVPLIEQLKLRSTIAGFANPKSSTGRLDVFTRLIADRQDGFDSV
                     EEGYSGPLFAEICPRTFAIKVRYGSRLNQVRFRRRVGQQLDAAEQPGRVGDRALRKIH
                     SDVGLVEGEAAIREGLNLRVSLAPIPPSGLVGYRARRYAGVVDVDAIGAYEADQFWET
                     VHLDGDRRLVLDPHEFYILASKESVSVPPAYVAEMAPFDPLIGEYRVHYAGFFDPGFG
                     YAPGKAPGAKAVLEVRSLDIPFILEDGQIVGRLVYDKLTEFPDQVYGQGIGSHYQAQG
                     LKLSKHFKQD"
     gene            3203..3637
                     /locus_tag="VFY74_10560"
     CDS             3203..3637
                     /locus_tag="VFY74_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEX5932170.1"
                     /translation="MLIGHLALAAAALFTGAAFYINFAEQPARLALDDRSLLAEWKPA
                     YARGFVMQATLAVVGFVLGAVAWWQTGQLAYLAGALLMLANWPWTLLVIKPVNDDLMA
                     TDPADAGPQTRANIVKWNTLHAVRTVLGALAAICFLFAVSAD"
     regulatory      3678..3755
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            3843..>4631
                     /locus_tag="VFY74_10565"
     CDS             3843..>4631
                     /locus_tag="VFY74_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5932171.1"
                     /translation="MGASKNRTGKTLPWHEATALIHGGTLRSQFGETSEAIFLNSGYV
                     YATAEQAEARFKGDEEGFVYSRYANPTVAMFEARMCALEGAPAARGTASGMAAVAASL
                     LCHLKAGDHVISARALFGSCRYVVEDLLPRLGIATTLIDGRDLGAWEAAVKPNTRAFF
                     FETPTNPVLELVDIEGVAKIAKRAGALVVVDNVFATPLLQRPMPLGADIVVYSATKHI
                     DGQGRCLGGIVLGPEEYIEEKLHNYLKHTGPSLSPFNAWVLLKGL"
BASE COUNT          763 a         1532 c         1501 g          835 t
ORIGIN      
        1 cttccacgtc catcgcgcgc agcgcatcgg cgcgcacctc gggagcggga tgaaaaccgt
       61 cgacgatcgc cagaactcta ttccagtgct ccgctcgatc atccgcctcg gtcggcatgc
      121 tcacatgtcg catcgcttgt cgcgcgggct tgccgtggtt gctcgcggcc cgttggaatt
      181 ccctggggtt gcttgtagca gaacgggtgc gacatgagaa cacgaaaacg gccccgcgga
      241 ggagccgtga tcgtcgccaa ctacttgatg cggctccgga aaattttgga gcgggtgaag
      301 ggaatcgaac cctcgacacg tagcttggga agctactgct ctaccactga gctacacccg
      361 cgttctccgc gagtcctagc atgatgggcc gggtgcgagg aagcgcgcct gcgcgaaccg
      421 gtctgaccga tccgattccg gctcatgcct cggggcgccg tctcgaaggc ctcatcgaga
      481 ggaggcgcgg gcggcttgcc gcacgcgccg cctcgaagga tgcggttaga gatgctgccg
      541 attggccatt ggtccctgtg gcgtcatgct tcgagacggg cccttcgggc cctcctcagc
      601 atgagggatg actttgggag cgcgcgcgcc tcacagagtg gtgatgcttg gccccttgcc
      661 gcgccctgag aacctcctct taatgcctgg cggaggaagc gagatggccg aggccgcgct
      721 ccgcgctcac ccgccgaaat tcagcgaccc cgacctcacc gccacgggcg aacgccgcgc
      781 gcgggtcgcg ctgaaggcgc tccgcacgct ttggatcaat accggcagcc tctgcaatat
      841 cacctgccgc aattgctaca tcgaatcgag ccccgagaac gaccggctcg cctatatcac
      901 gcgtgccgag gcggccctct atctcgacga gatcgcccgc gatggctggc ccgtcaccga
      961 gatcggcttc accggcggcg agcccttcat gaatcccgac ttcatcgcga tgctcggcga
     1021 cgcgctatcc cgcggtttct ccgcgctcgt gctcaccaat gccatgcagc cgatgctgcg
     1081 gccccgcgtc atggcggggc tgcttgcgct tcgggacgcc catcgcgacc gcctcgtcat
     1141 gcgcgtgagc ctcgatcact attcgctaga gctgcacgac accgagcgga gcgaaggttc
     1201 ctatgccaag acgatcgaag gcatcgactg gctcgcgcgc gaaggcttca agctcgcgct
     1261 tgccggccgc acctgctggg gcgagagcga agcggatctg cgcgacggct acgccgcgct
     1321 gatcgccgcg cggggctggc ccgtcgatgc ctatgatccc gcggcgctcg tgctcttccc
     1381 ggagatggac gcacgcgccg acatgcccga gatcaccgaa gcgtgctgga gcattctcgg
     1441 caagagcccc gcggacgtca tgtgcgcctc gagccgcatg gtggtgaagc ggaagggcgc
     1501 ggccgcgccc atcgtgctcc cctgcacgct gctgccgtac gatcccgcct tcgagatggg
     1561 cgaaacgctg gctgaggccg cgcgcgccga tggcggcatg ttcgcgggag gagccgtcaa
     1621 gctctgccac gtccattgcg ccaagttctg cgtactcggc ggcggcgcct gctcggcgtg
     1681 agcatttttc gtcacgcgtc aagcgcgcgc atgaggaagc gagactttct atcatctatc
     1741 ccaacgttgt catggccggg cttgtcccgg ccatccacgt cttcgcgatt caataaggaa
     1801 ttcgtggatg cccggcactt cggccgggca tgacgaagtg ggagcttcgt attgtccatt
     1861 gaagtgatcg gccttgagct tagctgcgct cagtggcaga gccctagtcc tgcttgaagt
     1921 gcttcgacag cttcaggcct tgcgcctggt agtgcgagcc gatgccctgg ccgtagacct
     1981 ggtcggggaa ctccgtgagc ttgtcgtaga cgaggcggcc gacgatctgc ccgtcttcca
     2041 ggatgaacgg gatgtcgagg ctgcgcactt cgagcaccgc ctttgcgccc ggcgcttttc
     2101 ccggcgcata tccgaagccc ggatcgaaga agccggcata gtgcacgcgg tattcgccga
     2161 tcagaggatc gaacggcgcc atctccgcga cataggccgg aggcactgac acgctttcct
     2221 tcgaggcgag gatgtagaac tcgtgcgggt cgagcacgag gcggcggtcg ccatcgagat
     2281 gcacggtctc ccagaactga tcggcctcgt aggcgccgat ggcatcgaca tcgaccacgc
     2341 cggcatagcg gcgggcgcga taacccacga gccccgaggg tggaatgggc gcgaggctga
     2401 cgcgcaaatt tagcccttcg cggatcgcgg cctcgccctc gaccaggccg acatcggaat
     2461 ggatcttgcg aagcgcgcgg tcgcccacgc ggcccggctg ctcggcggca tcgagctgtt
     2521 gtccgacgcg ccgccggaag cggacctgat tgaggcgcga gccgtagcgc actttgatgg
     2581 cgaaggtgcg cgggcagatc tcggcgaaga gcgggcccga atagccttcc tcgacgctgt
     2641 cgaacccgtc ctgacggtcg gcgatcagcc gggtgaacac gtcgagccgc ccggtcgagc
     2701 tcttcggatt ggcgaagccc gcgatggtcg agcggagctt gagctgttcg atcagcggca
     2761 cgagatagac gcagcctgcc tcgagcaccg cgccgcgcgt caaatcgatc tcgtggaatt
     2821 tcagctcggc gagcttgtcg cgcaccgtgg cgtcgggacc cggcaggaag ctcgccctga
     2881 tgcgaaaggc gaccttgccc aatctgaggt cgaggctcgc cggctgaatc tgattggtct
     2941 cgatctcggg cgcgcctgcg atctcgccgt ccgcgatcat ggcgcggatc gcctggctcg
     3001 gcaggatgcc ggtccgtgca aacgcgcttc tcggggcatc gcttggcgcg cccgcagggc
     3061 gatcctcagg caaagcctcg aaaagccgat cgcgctggct cgctgatgac atggcaggtc
     3121 tttgcatttt tcttgtgtcg cgtcatccta agctcgccct tctcccccgg attcacaagg
     3181 gggtggggtc aggacggaaa ccatgctgat cggccatctc gctctcgccg ccgccgcctt
     3241 gttcacgggt gcggccttct acatcaattt cgccgagcag ccggcgcggc tcgcgctcga
     3301 cgaccggtct ctccttgccg aatggaagcc cgcctacgcc cgtggcttcg tcatgcaggc
     3361 gaccctggcg gtcgtaggct tcgttctcgg cgcggtcgcc tggtggcaga cgggccagct
     3421 cgcttacctc gccggcgcgt tgctcatgct ggcgaactgg ccctggacct tgctggtcat
     3481 caagcccgtc aacgacgatc tgatggcaac cgatccggcc gatgccgggc cgcaaacccg
     3541 cgccaacatc gtcaagtgga acacgctgca cgcggtgcga acggtgctcg gcgccctcgc
     3601 cgccatttgc tttctcttcg ccgtgtccgc cgattgatct ggccgggctt gacggcgcgc
     3661 ccgcgtaggg acatgaatcc cctccgcgtg gtgatttggc cggccggctt gcagccacgt
     3721 taaacaaatc gctaaaaggc cgcgcgaggc gatgagaagc cgatcccggt cttttggcgg
     3781 aagccagagt ccgggttttt tgttgcggcg gcgcgcgggc cgcgttgtta aggagcatca
     3841 ccatgggcgc gagcaaaaac cgcaccggca agacccttcc ctggcacgag gcgaccgcgc
     3901 tgatccacgg cggcacgctc agaagccagt tcggcgagac ctccgaggcc atcttcctca
     3961 attcgggcta cgtctacgcg acagccgagc aggccgaggc gcgcttcaaa ggcgacgagg
     4021 agggcttcgt ctatagccgc tacgccaacc cgaccgtcgc catgttcgag gcgcgcatgt
     4081 gcgcgctcga gggcgctccc gccgcgcgcg gcacggcgag cggcatggcc gcggtcgccg
     4141 cgtccctgct ctgccatctc aaggccggcg atcatgtgat ctcggcacgc gcgctgttcg
     4201 gcagctgccg ctatgttgtc gaggatcttc tgcctcggct cggcatcgcc acgacgctga
     4261 tcgacgggcg cgatcttggc gcctgggaag cggcggtaaa gccgaacacg cgcgccttct
     4321 tcttcgagac gccgaccaat cccgtgctcg agctcgtgga catcgaaggc gttgcgaaga
     4381 tcgcgaaacg cgccggcgcg ctcgtcgtgg tcgacaatgt gttcgccacg ccgttgttgc
     4441 agcggccgat gccgctcggc gccgacatcg tcgtctattc cgcgacaaag catatcgacg
     4501 ggcagggccg atgcctcggc ggcatcgtgc tcggacctga ggagtacatc gaggagaagc
     4561 tccacaacta cctcaagcac accggaccct ctttaagccc gttcaatgcc tgggtgctgc
     4621 tgaaggggtt g
//
LOCUS       DASUQP010000231        38106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1067540, whole genome shotgun sequence.
ACCESSION   DASUQP010000231 DASUQP010000000
VERSION     DASUQP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 38106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38106
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067540"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFY74_10570"
     CDS             <1..825
                     /locus_tag="VFY74_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX5932172.1"
                     /translation="VNMDFSDERDMVAKLRTGLALQPVATALFANSPFTDGKPNGFRS
                     YRAEVWRDTDPDRTGLIPFAFEPGMGFERYVDYGLDVPMYFVYRDGRYIDVAGASFRD
                     FLAGKLAALPGLRPTLDDWADHLTTLFPDVRLKHFLEMRGADAGALAQVWALPALWTG
                     LLYDKEALEGAAALTTDWTEAERQALRDTVPRLGLETPFRGDTLRDVAREVVALAERG
                     LKRRARLDRKGEDERKALAPLIETIEDGGSPADRLLALYHGPWQGDIDRVFDTQAF"
     gene            complement(1106..1609)
                     /locus_tag="VFY74_10575"
     CDS             complement(1106..1609)
                     /locus_tag="VFY74_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX5932173.1"
                     /translation="MSVTLAGQVQAQGAASEAAPAATGPGSVVRETHGAWQVSCRTPP
                     GAKEEKCALVQSVTAEDRPNVGLTVVFYKAIGEDKKLLRVVVPLGVLLPTGLGLKIDG
                     QDVGNAPFLKCGKRGCVAEVVLQDDIITKMKKGTTAMFIIFDTPEAGIGIPVALQGFG
                     DALANLK"
     gene            2166..2993
                     /gene="coxB"
                     /locus_tag="VFY74_10580"
     CDS             2166..2993
                     /gene="coxB"
                     /locus_tag="VFY74_10580"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5932174.1"
                     /translation="MAICQRITTAFGAAASFALCGAAYAKDGQPVPWQIDFQPAASPI
                     MEQIHSFNTYVTIIITLITVFVLALLVYVMVRFNERRNPQPSRTTHNTVLEIAWTIIP
                     VLILVAIAIPSFRLLFAQYDFPKADLNITATGSQWYWTYEYPDHGITFDSIMVPEADL
                     KEGQPRLLAVDREVVVPVNKNVIVGLKSNDVIHDWALPAFGVKLDAVPGRLQSTWFRA
                     QREGVYYGQCSELCGRNHAFMPIAVRVVSEAEFADWLGKTKAAAAPGNAGQVAASQE"
     gene            3069..4673
                     /gene="ctaD"
                     /locus_tag="VFY74_10585"
     CDS             3069..4673
                     /gene="ctaD"
                     /locus_tag="VFY74_10585"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX5932175.1"
                     /translation="MAHPAHAEEARHDHHPTGLGRWFFSTNHKDIGTMYLVLAMIGGI
                     VGGLLSMGMRAELMEPGMQIFGNPEVFNVFVTAHGLIMVFFMVMPATMGGFGNWMVPL
                     MIGAPDMAFPRLNNISFWLLAAAFSLLVTSLFVPGPPGTKGVGGGWTVYAPLSTKGTP
                     GPAMDFAILSLHLAGASSILGAINFITTIFNMRAPGMTLHKMPLFVWSVLVTAFLLLL
                     SLPVLAGAITMLLTDRNFGTAFFDSAHGGDPLLWQHLFWFFGHPEVYILIIPGFGMVS
                     QIVSTFSKKPVFGYLGMAYAMVAIGVIGFIVWAHHMFAAGISVNTQAYFVFATMVIAV
                     PTGVKIFSWIATMWGGSLSLRAPMLWALGFIFLFTIGGVTGVVLANAGVDRSFHDTYY
                     VVAHFHYVLSLGAVFSLFGAWYYWFPKMYGYMYSEALAKLHFWLMFIGANLTFFPQHF
                     LGLAGMPRRYADYPDAFAGWNYISSLGSYLSFAGFLVFLFGIFYAFARKQHAAANPWG
                     AGATTLEWTVPSPPPFHTFETLPRIK"
     gene            4738..5694
                     /locus_tag="VFY74_10590"
     CDS             4738..5694
                     /locus_tag="VFY74_10590"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX5932176.1"
                     /translation="MADAAISVATGTADIDAGGDVADYVALMKPRVMFLVVFTALVGL
                     VAAPVSLHPVLAIASLICIAAGAGAAGALNMWYDADIDAKMARTAARPVPRGRVTAEE
                     ARSFGAVLAVGSVICLGVMANWLSAVLLAVTIGFYVVVYTIWLKRLTPQNIVIGGAAG
                     AVPPMIGWAAATGGISLESFVLFLIIFVWTPPHFWALALLRTHDYAKAGVPMLPVVAG
                     PDATRVQILIYSLMLAPLGMTPAIIGLGGILYAVGASVLGSLFVVMALTCYREREGEA
                     ADRAAKNLFGYSVLYLFLLFAALLVERGFGVDGGSLLSLWTS"
     gene            5853..6443
                     /locus_tag="VFY74_10595"
     CDS             5853..6443
                     /locus_tag="VFY74_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5932177.1"
                     /translation="MSETGQARDQKPKRHGAVALALVGLVAVMTGLSFAAVPLYRLFC
                     QVTGYSGVPQRAEAAPERGLDRAIRVRFDANVDRALPWRFAPVERVIEVKIGETALAF
                     FKAANQSKTPVTGTAVFNVVPERAARYFNKIECFCFKEQTLAPGASVEMPVTFFVDPK
                     VVEDEDTKNISEITLSYTFYRAGEEAGLAAAGKSGS"
     gene            6498..7382
                     /locus_tag="VFY74_10600"
     CDS             6498..7382
                     /locus_tag="VFY74_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5932178.1"
                     /translation="MAEAHAKQHDYHLVDPSPWPIVGAVGALTLAVGTIMFFISKKAG
                     TPQLWYVAPGLVLVIITMFGWWRDVIKEAKTGYETPVVQLHLRYGMILFIASEVMFFV
                     AWFWAYFDAALYPDDAITYARTQVLGGQWPPIPSAETDVPGLGSFRHTFDPWGLPFVN
                     TLILLTSGTTVTWAHHALLHNDRRGLVLGLLCTVVLGILFTACQAYEYMHAGFGFANH
                     IYGSTFFMATGFHGFHVIVGTIFLTVCLIRAMRGDFTPKMHFGFEAAAWYWHFVDVVW
                     LFLFTCIYVWGASSAAGH"
     gene            7451..7840
                     /locus_tag="VFY74_10605"
     CDS             7451..7840
                     /locus_tag="VFY74_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX5932179.1"
                     /translation="MTRGEARPNVPSLGSTLAAGIAGRCPACHRGKLYSGYLALAPRC
                     ENCGLDYSFADSGDGPAVFVILVTGFVVTGLALLAEIFYAPPYWLHAVLWGSLAILLP
                     LVLLRSFKGALIALQFRHKAEESKFAE"
     gene            7852..8589
                     /locus_tag="VFY74_10610"
     CDS             7852..8589
                     /locus_tag="VFY74_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX5932180.1"
                     /translation="MSKGLLGFTVLMLLALSVLVGLGVWQLERLQWKEGLIAKIEART
                     KAQPIGLNDAAAMAEEGRDPSYYRVRVSGRFDHAKERYLFAVSEGRGGWHVIAPLKTV
                     DGETVLIDRGFVPDSLKNPSARPEGEVQDMVTVTGIVRLPETRHTFTPENEPEANRWF
                     WRDLESMARSMFPAEAVRLAPFFLEADKGEVPGGWPEGGQTRLDLPNNHLQYAVTWFL
                     LAACLLVIYGAYVWKAKARRWATGEKG"
     gene            8674..10116
                     /gene="thrC"
                     /locus_tag="VFY74_10615"
     CDS             8674..10116
                     /gene="thrC"
                     /locus_tag="VFY74_10615"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5932181.1"
                     /translation="MKFISTRGEAPALSFEGVLLAALARDGGLYLPEAWPSLEAEAIE
                     GLAGLDYADAASRVMRPFLAGDPCLADLDAVLEEAYGSFHHPAVAPLRQIAPNSFILE
                     LFHGPTLAFKDLAMQVVARLMNRALLRKGAHATVIGATSGDTGAAAIEAFRGLEAIDI
                     FILHPKGRISDVQRRQMTTASEANVHNIAVEGTFDDCQTIVKGLFGDLSLRDRLALTG
                     INSINFARILAQIPYYFTAAISLGAPRRAVAFTVPTGNFGDIFAGYAAARMGLPLARL
                     VIATNLNDSLVRALGSGVYAPMSVIATSSPSMDIQLASNFERLLFELAGRDAARVRSL
                     MDALRKTGAFRLNDAELSSLRGQFSAHAIGEPETEMTIRSLAEETGVLADPHTAVAVA
                     AARAESGQGSWPMVVMSTAHAAKFPEAVERASGRVPEQPERLRLRLGQHERCAVLPND
                     YRAVAAFIEANARAEGRNSARARRRTGVSA"
     gene            10113..11393
                     /locus_tag="VFY74_10620"
     CDS             10113..11393
                     /locus_tag="VFY74_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5932182.1"
                     /translation="MSLHQSKLANGLTVVSHAMPEVETVSLGLWVGAGSRSEAPGEHG
                     VAHFLEHMAFKGTARRSAQDIAEEIEAVGGELNAATGVDSTAYYAHVLRKDMPLALDI
                     LSDIVVAPRFDKIELARERDVILQEIAAAMDSPDDIAFDLVQEAAYPGQALGRPILGT
                     AESVSRVKRDHLGAYLSAHYHGPNMVLAAAGAIDHGALLAEAERGLGSFDPMPAAPPE
                     AATYTGGIRRSDKTFEQTHVVLAFEGPHYRHPDYFTAQICAGALGGGMSSRLFQEVRE
                     RRGLCYAIYAFASGISDSGMLTIHAAGSSDRAHNLFAVIRDELVKAAEKGFRADEVAR
                     VKAQLKMGLLTGLESSSARAEQLARQILIHGRPLSSEELIEKVDRVEAHDMQALIERM
                     LASPVSLATVGPILHVARFDRVTEKFAVPSTKAA"
     gene            11409..11996
                     /locus_tag="VFY74_10625"
     CDS             11409..11996
                     /locus_tag="VFY74_10625"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5932183.1"
                     /translation="MALLRAVSTSDLGPVIESDAVTLRTPQMSDYPEWAELRAQSREF
                     LSPWEPLWAADELSRASFRRRVRHYLRDLREDVGYALFIFAVPTGALVGGLTLCNVRR
                     GVTQSCTLGYWIGAKYANQGYMTAAVRAVVPFVFDSLELHRLEAACLPSNTASIRLLE
                     RTGFRREGLARRYLRINGVWQDHLLYALLDTDTCR"
     gene            12069..15086
                     /locus_tag="VFY74_10630"
     CDS             12069..15086
                     /locus_tag="VFY74_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5932184.1"
                     /translation="MRRFVGPIGAVLCAVAALLVWAPPALAIEAIPVEPNQDKIDITA
                     KGELYEGRGDRLQIETAPGPDGYIGRMAVSASTPGTNPAWLVFALSNPTSERIVRWLV
                     APRYTLADSRVLWPILDSPRIAAITPSLGFRPEPLKSDRADMFRLSLEPGQTITFAAE
                     LSSQQVPLLNLWDPNTYQAKQQDRMLFNGVLLGITGLLAIFLTAIFAANHRAIFPATA
                     LIAWAALAYFCVDFGFWNKLFPVGPDRTALYRAAAEAGLAASLVLFLYAFLRIGLWHG
                     WIRTLFWGWIAFQLSLVFLAIVDPASASGLARVSLVAIAGVGAALILYFALRGQDRAL
                     SLIPSWMLLIVWLFGAAMIVLGHLSGDLVVSGLIAGLVLLLVLLGFTVTQFAFRSGAT
                     PLSLGPPSLVQLKSLAVDGSGAYVFQWHAGRDEITVANEVEHELGLEPGALSVAVDDF
                     LQYVHNADRERVRLMLWSLQEKQGGDIQVEFRLRRHDGAYLWYELRAHAAPSENPRLL
                     RCTGLMRDVTNLKRSHERLIHDAVHDSLTGLPNRELFMDRLQGAITRAREGQANRPTV
                     LVIDIDRFKNVNKSFGLVIGDSMLLTLGRRLSRHLNPQDTLARLGGDQFAILLISDAD
                     PRNVATLAERVRRALRTPMKISAKEIILTASIGIVVHDGSQETAQEMLSEGELAMLRA
                     KRAGADRIEIFNPAMREEDESKLPLESDLRKALERQQIAILYQPITRLASNQLAGFEA
                     LMRWDHPTRGRLGAEDFIPIAEESGLIVELGSYVLSQALEEARSWHKALPRDRDPLFV
                     SVNVSSRQLFRQDMVQEIRLMLAREAVPKGTLKLEITESLVMENPERAVEILTWLKTF
                     GASLALDDFGTGYSSLSYLHRFPCDTIKVDRSLVRNSGIDGSSPLILRSVIALAHELG
                     KEVVAEGVETQADASYLRSIGCEYGQGYYYGEPMPAKDVANLLAALASHRKRRERERA
                     RAPAQPPQGLDVQVAGPGPLPALQGPGTLPSTS"
     gene            complement(15206..15820)
                     /locus_tag="VFY74_10635"
     CDS             complement(15206..15820)
                     /locus_tag="VFY74_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5932185.1"
                     /translation="MPDRSAPNRPKGTGYLDGQLLIAMPAMGDPRFARSVIYLCAHSA
                     DGAMGIIINQRAPNISFTELLEQLNIVPTQDRISLPAGLHAMAVHLGGPVETGRGFVL
                     HSADYFKAESTLPIDESVCLTATIDILRDIAKGTGPDKALLALGYAGWAPGQLESEIH
                     ANGWLNCPADPGLIFDPDVDRKYLRALGKLGIDPIRLVNDSGHA"
     gene            15985..16842
                     /locus_tag="VFY74_10640"
     CDS             15985..16842
                     /locus_tag="VFY74_10640"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX5932186.1"
                     /translation="MRSLLLPRRLFVPLLTVAAILALDAAALGGAPSSSPWTASTNSK
                     VRLVSGTVVNGSGTALAAGVQLRMEPGWKTYWRNPGDSGVPPSFDWTGSKNLKGAEVL
                     YPAPHRFADAGGTAIGYGEDVLFPVKLTPERDGEPIEVKLAFFYGLCKDLCIPNEVNL
                     SLALPSDEEKGEALILDAALAQVPKPARSGLLPEVNGVEAKLDGDAPGLVVDAVFPPG
                     ATGTDLFIDGGDVFVPVPAPLGALVDGKQRFAVTFASPSEAAAIKGKTLRLTLVSDQG
                     STETMWKAE"
     gene            16937..17422
                     /locus_tag="VFY74_10645"
     CDS             16937..17422
                     /locus_tag="VFY74_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5932187.1"
                     /translation="MTIAVGDRLPDATFRFLGPDGITSTTTRDVFGGKKVVLFAVPGA
                     FTPTCHLKHLPGFIERAAEFKEHGVDTVACVAVNDPFVLGAWEEKSGGKGKVQFLSDG
                     NAEFTKKIGMDFDGSGIGLGTRSKRYAALVDDGVVKILNVEESPGVAAESSADKILED
                     L"
     gene            complement(17437..17904)
                     /gene="rnhA"
                     /locus_tag="VFY74_10650"
     CDS             complement(17437..17904)
                     /gene="rnhA"
                     /locus_tag="VFY74_10650"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX5932188.1"
                     /translation="MGARPKVVIHTDGACSGNPGPGGWGVILQSGAHRKELMGGEALT
                     TNNRMELMAAITALESLKAASEVDLYTDSNYLRGGITSWIKSWKKNGWRTADRKPVKN
                     AELWERLEKAASRHTVRWHWVKGHVGHDANERADELAREGMAPFLEGRRLRPE"
     gene            complement(17907..18875)
                     /gene="thrB"
                     /locus_tag="VFY74_10655"
     CDS             complement(17907..18875)
                     /gene="thrB"
                     /locus_tag="VFY74_10655"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5932189.1"
                     /translation="MAVYTDVSDEELEAFIGSYAIGALTSFKGIAEGVENSNYLVHTE
                     SGRYILTLYEKRVSREDLPYFLALMQHLAARGITCPLPVKDRDGQTLKVLAGRPAALI
                     TFLDGLWVRRPGIEHCAGLGDALARFHLAGRDFPMARANSLSLPGWQALFAMIGGGAE
                     RVAQGLSGVIEKELRHLEANWPKDLPEGVIHADLFPDNVFFLGDKISGLIDFYFACND
                     MLAYDVAVCLNAWCFETDGSFNVTKARALLEAYEAVRRLTRAELDRLPTLARGAALRF
                     LLTRTYDLINTDANALVKTKDPGEYLRKLRFHQRVKSTRDYGLKER"
     gene            complement(18876..19868)
                     /gene="ispH"
                     /locus_tag="VFY74_10660"
                     /gene_synonym="lytB"
     CDS             complement(18876..19868)
                     /gene="ispH"
                     /locus_tag="VFY74_10660"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5932190.1"
                     /translation="MTGITKAKGRHSDRPSLTIYLAAPRGFCAGVDRAIQIVELALAK
                     YGAPVYVRHEIVHNHYVVESLKAKGAIFVEELDEIPDNGAPIVFSAHGVPKAVPAAAR
                     ARNLFFLDATCPLVSKVHVEAERQFREGRDILLIGHDGHPEVVGTTGQLPDGAVILIE
                     TVEDAQAFVPRDPDRLAVITQTTLSVDDTKEILNALKARFPRMVTSHKDDICYATTNR
                     QEAVKRIAPACERMIVVGSPNSSNSIRLVEVAERAGCPAAVLIQRAAEIDWAMFNGIS
                     RLGVTAGASAPEVLVDEIIDAFKARFETTVETVTTAEESIAFKLPRELREMPAL"
     gene            20055..20678
                     /locus_tag="VFY74_10665"
     CDS             20055..20678
                     /locus_tag="VFY74_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932191.1"
                     /translation="MRRTDARSWLSRCALAGLVAVPLFVSGCSVPRFSNPFGSDDDDT
                     SAVPQTSMLENAKVDVASLPSGYSANCPQMVAWPRDRMLTVYQPDHIGDNNAVVHRGE
                     ITKMSRECQLHADRVVVKYGFAGRVLLGPKGGPGTVSFGVSLRVAGVQQNTLATDKMK
                     VTTTIPPGGPAGYFSMVGQIAFPIVVGTRPQDYKIFLAFDRSVAGAG"
     gene            20837..21541
                     /locus_tag="VFY74_10670"
     CDS             20837..21541
                     /locus_tag="VFY74_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5932192.1"
                     /translation="MRDLYRILGIGREAARDEIHKAYRRKAKASHPDSGGSVGAFGEL
                     ATAYAVLSNPSCRERYDTTGQVEPPRPNNFDASAVEVIAQKLGLIIHAEQDMTDMDIG
                     ALIEAAIGEDMAERRANIASQRRAIGRAERLRSRIKRKANGNDNMLARVLDWHESSTR
                     MQIDKNEAAVASMERALEILKDYSFTEDLPRGAAGDEVSLALNDALHSLDQLAAVLKT
                     GPAWCDLDIARPSASG"
     gene            complement(21600..22091)
                     /locus_tag="VFY74_10675"
     CDS             complement(21600..22091)
                     /locus_tag="VFY74_10675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949765.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HEX5932193.1"
                     /translation="MEVLMTPEFWVAVSFFLFVGALLYFGVHKKLASVLDARAAAIAK
                     ELDEARRLREEAEKVLADYRRKEGDAAKEAQAIIDLAAKEAEILAAETHRSMTEHFER
                     RMKLAEDKIARAEADALREVRAAAADAAIAAARTVIAEKLTPEAADKLVKQGIDALKG
                     RLN"
     gene            complement(22094..22579)
                     /locus_tag="VFY74_10680"
     CDS             complement(22094..22579)
                     /locus_tag="VFY74_10680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B' is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HEX5932194.1"
                     /translation="MPQLNPLDWGPQLLWLLITFGILYLLMVYVALPRIGSVIEARAA
                     RIAKDLATADKLRRETEEAIAAYEQALAEARQKAHAIIEQGRDKLKAETAAERARLDG
                     DLAERGAEAEARIGAAKAKAMKDVNTVAADVAADIVRRLIGVAPAKAEIDKAVGTARK
                     G"
     gene            complement(22622..22849)
                     /locus_tag="VFY74_10685"
     CDS             complement(22622..22849)
                     /locus_tag="VFY74_10685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5932195.1"
                     /translation="MDPAAAKLIGAGLASIALVGAGVGIGTIFGNYLQGALRNPSAAP
                     SQFANLLLGFALAEATGLFGLVVALIILFVF"
     gene            complement(22893..23621)
                     /locus_tag="VFY74_10690"
     CDS             complement(22893..23621)
                     /locus_tag="VFY74_10690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5932196.1"
                     /translation="MPDPMHQFEIKRLIPLELFGYDASFTNSALFMVIAATLITLFTV
                     YAMRGRALVPTRLQSVAELSYEFVANMVRDNVGTAGMKYFPFIFTLFMFVLTLNMLGM
                     LPYSFTVTSHIIVTFALAAFIFLSVTLIGFVKHGVKFLKFFVPDGVPFVMLPLLVVIE
                     VISYLTRPLSLSVRLFANMMAGHTMLKVFGAFVVALGFLGGWAPLAFMVAFTGLEILV
                     AFLQAYVFAILTCIYLNDALHMHH"
     gene            complement(23691..24080)
                     /locus_tag="VFY74_10695"
     CDS             complement(23691..24080)
                     /locus_tag="VFY74_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5932197.1"
                     /translation="MAGTKNQSGRREVRRDTGEIRQRLDALGEKLDEAQSRHGPVHAD
                     APPSQGAALGQALKLGVELVAGVAVGFFIGWWLDRLFGTAPFLMVVFLGLGAAAGILN
                     VVRTAKRMQAEAPSGKDLPSVNDDDDD"
     gene            24295..24516
                     /locus_tag="VFY74_10700"
     CDS             24295..24516
                     /locus_tag="VFY74_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX5932198.1"
                     /translation="MNAKTKALSEEARKLSPDERIALIEDLQRSLVPTDPEIDRAWAE
                     EARDRLNAYRRGEIEVMTLEDVIASLTKR"
     gene            complement(24816..28265)
                     /locus_tag="VFY74_10705"
     CDS             complement(24816..28265)
                     /locus_tag="VFY74_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HEX5932199.1"
                     /translation="MKITRLRLLGFKSFVEPTELVIETGLTGVVGPNGCGKSNLLEAL
                     RWAMGETSYKSMRGAAMEDVIFAGSEDRPPRNSAEVTLFIDNSARKAPAEFNDSDALE
                     VTRRIEREAGSAYKVNGRDVRARDVRLLFEDAATGARSPALVRQGRIGEIVNAAPQER
                     RRVIEDAAGIAGLHSRRHEAELRLAATEGNLTRLSDLIGQLASQLQALKRQARQAQRY
                     REVSGKIREAEALQHHLNWVGACAHVDEEEAAFQQALAAVAQETKAEGEALRQQAEAA
                     EKLQPLRDEEAARAAVLHRLSVERDALEREEARAKARQAELETRLAQASRDLAREEEH
                     IAEADGMLRRLGEEKRRLEAASSRAHEEPQLRAASAKAKDALDAAERTLREATTALAE
                     ARAERGKHEGEKVELLARMAKLEAQAAEIEAELATRQAESESGGKLQALNAGLAEFSA
                     NVQRLEVELQAAEAEEEAARLSEAEARATASAAKLASQELETELATLIKLLAPAHDWS
                     PIVAEIKITSGYEQALGAALGDDLDAASDEKAPAHWRLTAPSGGDPELPEGATPLSKF
                     VKGPEVLARRLRQIGVVDAARGRKLQASLAPGQRLVSKDGDLWRWDGYSLKAGTDSAA
                     GARLLERRRLKALHGEAAKAQARAAATESALRAASERAERAALATKALRQEAKDAHAQ
                     LDRARDAIAASEHAAQAASKELGARTEALSRTRAAFDEAQAKSARAASALLELASLSG
                     LETALQAALARADQGRQAAARADAALEGFERELRLRQERMEAIGEEEELWRKRIANAR
                     EQILTLRAREEETSADLAALANLPAEIDQRRMKLFDAIAGAEHERSKAADELALAETA
                     LKEREKALREVQERLAGAREARARNEARLEAARERRSDAARTIRDQLDCAPEACLALA
                     GVKEGAPLPPLAEVEARLLKLRGDRERLGGVNLMAEEEEAQLAAQLEEMEREKADVEA
                     AILRLRQGIANLNREGRKRLLEAFNAVNSHFGRLFKTLFGGGSAELKLVDSDDPLDSG
                     LEIFACPPGKRLQALTLLSGGEKALTALALIFAVFLTNPSPICVLDEVDAPLDDANVE
                     RFCLLMEEMAKDTDTRFLIITHHPLTMSRMSRLFGVTMAERGVSQLVSVDLEHAERLR
                     DAS"
     gene            complement(28389..29057)
                     /locus_tag="VFY74_10710"
     CDS             complement(28389..29057)
                     /locus_tag="VFY74_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5932200.1"
                     /translation="MRASAAKPSIALAMALALAGCGGGAGGPTPPIDKATYVPPQAEL
                     MATGPLGERALGNPNAPITVIEYVSLTCPHCANFHKTLFPRVKKEFIDTGKVRFIVRE
                     FPIGRTSGNAAIANRCAPEDKYFFLLGQFLTRQPEWVSQDVRPDAIFAVAKSSGMTRE
                     TFDKCLSNQTIIDGLNEVKQRGRQFGVIGTPTFFINGRKAQGEVTFEEIKAMIEQAPA
                     PSSS"
     gene            complement(29069..29872)
                     /locus_tag="VFY74_10715"
     CDS             complement(29069..29872)
                     /locus_tag="VFY74_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5932201.1"
                     /translation="MAKKEKKAAGKSPRGETPGGQDAAAANSIWLYVAGAVAAVALAI
                     GIFYWRDSSAGGAGGEKTDPGLAELMAPGPLPDLSLGKEDAPDTIVEYASMTCPHCAQ
                     FHKTVLPELKTKYIDTGQARLILREFPLDQLAAQASMLARCAGPDRYYPMVGALFDTQ
                     ETWAVAGTEGTDKLLQIAKQAGFSKEKFDQCIGDKALFEKIVEIRERGHETFGVDSTP
                     TFFINGKRLTGEQTVAGFEAMLSGGATPPAGPASAEGQAPSEPEAPSGQ"
     gene            complement(29977..30645)
                     /locus_tag="VFY74_10720"
     CDS             complement(29977..30645)
                     /locus_tag="VFY74_10720"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX5932202.1"
                     /translation="MSCFFGLSPAFLFCMNRTPAALLSEPWCKLDGIEASGGRDPSTV
                     SSIMDDSERRALARAHATARPIGAYTARALDPAARARGFATAALLAEWPSIVGAELAQ
                     FTMPDRVVWPRHRDDTGETVRHQGRRDEGAILILRVDGPRAIEVQHRSCQILERVNSH
                     FGYRAVAEMRILQAPVTRKPAPAAPAAPQPIDPDALPPSAAIEDAGLREALLRLASAG
                     PHRR"
     gene            30602..31765
                     /gene="mutY"
                     /locus_tag="VFY74_10725"
     CDS             30602..31765
                     /gene="mutY"
                     /locus_tag="VFY74_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX5932203.1"
                     /translation="MQKRKAGERPKKQDIAAALLAWYARERRELPWRAAPGETADPYR
                     VWLSEIMLQQTTVKAVIPRYAAFLRQWPDVKALATAELGQVLAAWAGLGYYARARNLH
                     ACARAVVEQHGGGFPESAAELRKLPGIGDYTAAAIAAIAFGKRATAVDGNIERVVARL
                     FAVTTPLPEAKAEIRALAETLTPAERAGDFAQAMMDLGATICTPRRPACALCPLRPEC
                     RGFADGVAEALPYREEKRERPTRRAAAFVALRPDGAVLLRKRPLKGLLGGMLETPSSP
                     WEEARPNGKLLGQYAPLEADWRKLPGLVQHSFTHFHLELTVYRADVGRSAKPKRAAVP
                     ELCGFVARRDLARAALPSVMRKVLNHAFQSKARTLKAGRQASPALPRARRRSA"
     gene            complement(31707..32813)
                     /locus_tag="VFY74_10730"
     CDS             complement(31707..32813)
                     /locus_tag="VFY74_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX5932204.1"
                     /translation="MRLGDKPGAQLPLDTVIVGDCIDALARLPEASVDLVFADPPYNL
                     QLNSELYRPDNSRVDAVDDDWDKFGSFSAYDAFTRDWLIACRRVLKPSGSLWVIGSYH
                     NIFRVGATLQDLGFWVLNDVVWRKTNPMPNFRGRRFTNAHETLIWASMGPKARYTFNY
                     EAMKALNDDLQMRSDWLFPICSGAERLREDCGRKTHPTQKPEALLHRIILASSNPGDV
                     VLDPFLGSGTTAVVAKRLGRAFIGIERDAAYAEAARARLKSLEGFDSAALQVAKGKRA
                     EQRIPFGALVELGLIAPGTVLYDPAARHEAKVRADGSIACAGAQGSIHKIGAQVQGAI
                     ACNGWTFWHYEAEGTLKPIDALREAARAKLAVQP"
     gene            33066..33821
                     /locus_tag="VFY74_10735"
     CDS             33066..33821
                     /locus_tag="VFY74_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5932205.1"
                     /translation="MTRDIVMLHGANEGSWCFDKFKAVFESVGWTCHAPDLIGHGTRA
                     TNSDKTLVGIGIADYQAELEAFLKTIPPRPVLLGHSMGAVLAQLLAAKGLARALVLVA
                     PGARAGILPQTEGEKKFGQDLMGLGAFWKTVINPDFGLARVLTLNRVPEAEQRAVFDR
                     FGPESGRAFFELFFWMFDSTRATVVDISSVTCPVLCLAGADDNLVSVATVRATAAGYP
                     GATLWELEGHGHMLVLEPGAEAIARRIADWIPS"
     gene            complement(33818..34510)
                     /locus_tag="VFY74_10740"
     CDS             complement(33818..34510)
                     /locus_tag="VFY74_10740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5932206.1"
                     /translation="MGLAAESLPVRPLPSFDFEAEAMGKYRGAVAGIDEAGRGPWAGP
                     VVVAAVILNPDRIPEGLNDSKLLTPQAREDRYAEIIATSIVSIVIGHVRRIDRDNILQ
                     ASLWGMRAAYRGLGVPAGSALIDGNIIPRRFPCPARALVGGDGLSLSVAAASIVAKVT
                     RDRLMVKLARRYRRYGWESNKGYGTQEHARAIEKHGVCTHHRRSFSPIERALRLLETG
                     ADELPVLGSLEI"
     gene            complement(34589..35713)
                     /locus_tag="VFY74_10745"
     CDS             complement(34589..35713)
                     /locus_tag="VFY74_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX5932207.1"
                     /translation="MSISLRKAPDSQGLPPEAGGVAAGRRHGRGAQTNRSGRYEPIGY
                     EAADDGWGSLAELEALTTEVQEVPARRIITRNESPDIGFDRSINPYRGCEHGCIYCFA
                     RPTHAFLGLSPGLDFETRLFAKTNAAQALERELAEPGYEPRTIAIGTNTDPYQPIERR
                     YRIMRRILEVLSAANHPVGIVTKSALVLRDLDLLTSMAKRGLVKVALSVTTLDRKLAR
                     SMEPRASTPDKRLETLQRLVDAGVPASVMVAPVIPGLTDAEMERILERAAAVGVKQAG
                     YVLLRLPLEIGDLFTEWLQANCPDRAKRVLSLMRSTRGGKLYDAKWGERQVGSGPYAW
                     MIGRRFELAAQRLGLNEDSVALRTDLFTPPSRPGQQLTLL"
     gene            35818..36507
                     /locus_tag="VFY74_10750"
     CDS             35818..36507
                     /locus_tag="VFY74_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5932208.1"
                     /translation="MISVVIPTLNAERSLAHSLAALVPAVVEGVVREAIVVDGGSTDG
                     TRVVAEAAGTHLIEAGRGRGTQLEAGASAAQADWLLFLHADTVLDPGWAEEAQGFIER
                     VESGRRGQAAASFRFALDDDGIMPRFVEFLVGLRCRLLGLPYGDQGLLISRRLYRRLG
                     GFRPLPLMEDVDLVRRLKRRELVMLKSRAVTSGERYRREGYLARSLRNLSCILLYYLH
                     VPPRVLARLYG"
     gene            36500..37162
                     /locus_tag="VFY74_10755"
     CDS             36500..37162
                     /locus_tag="VFY74_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5932209.1"
                     /translation="MARRSGGRKRGASFAKRLVIMAKSPDMGRVKRRLAGEIGESAAI
                     RFYRGCLSHTLLRLARDPRWLTVLAVDQGRGRARRFSPLRKRLTFLPQGDGDLGRRMQ
                     RLFTRLPPGPVVIVGTDIPSVRPGHIAEAFRLLGGADAVFGPAPDGGYWLVGLRRTPR
                     VPAPFAAVRWSGPHALADTLANLKGKRVAMAARLSDVDTAEDLRRESARSGRLISTIT
                     TL"
     gene            complement(37163..>38106)
                     /locus_tag="VFY74_10760"
     CDS             complement(37163..>38106)
                     /locus_tag="VFY74_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX5932210.1"
                     /translation="DASQVASLGTAGPQVASLDAKAMALGYDDKSLAPPAYGPGLAFW
                     ARGYGAWGDFDGNLNAASASRDLGGIVSGLDARVSGSWRLGFATGYSNSDVSVAARHS
                     GADVESFHLAGYGGGMAGPVALRGGGAWSWNEVDTSRAVIFPGFFEREKTSYDADTGQ
                     IFGEVAYPIVRGRTALEPFAGLAYVSIDPDTFRENGALAGLRGSGDDLDVGYSTLGLR
                     AATTMAWNSVTVTPHVSAAWQHAFDDVRTGAALAFASTGIGFTVTGVPLAEDTALIEA
                     GLDLGFGPYTTAGVSYSGQFGDNVEDNAVQGRFAWRF"
BASE COUNT         6299 a        12444 c        12358 g         7005 t
ORIGIN      
        1 gtgaacatgg atttcagcga tgagcgggac atggtcgcca aattgcgcac gggcctcgcg
       61 cttcagccgg tcgccaccgc gctctttgcc aattccccgt tcaccgacgg caagcccaac
      121 ggatttcggt cgtaccgcgc cgaggtctgg cgcgacaccg atcccgaccg caccgggctc
      181 attcctttcg ccttcgagcc gggcatgggc ttcgagcgct atgtcgacta cgggctcgat
      241 gtgccgatgt atttcgtcta tcgcgatggt cgctatatcg acgtggcggg cgcctcgttc
      301 agagacttcc tcgccggcaa gcttgccgct cttcccggcc tgcggcccac gctcgacgat
      361 tgggccgacc acctcacgac gctcttcccg gatgtgaggc tcaagcattt cctcgagatg
      421 cgcggcgccg atgccggagc gctcgctcaa gtgtgggcgc tgcccgcgct atggacgggg
      481 ctgctttacg acaaggaagc gctcgaaggt gccgcggcgc ttaccaccga ctggaccgaa
      541 gccgagcgcc aagccttgcg cgacaccgtt ccccgcctcg ggctcgagac gccgtttcgc
      601 ggcgatacgc ttcgcgacgt cgcgcgcgag gtggttgcgc tggcggagcg aggcctaaag
      661 cgccgggcgc gtcttgaccg aaaaggcgag gacgaacgca aggcgcttgc gccgctgatc
      721 gaaacgatcg aagacggtgg ttccccggcc gaccgcctcc tcgccctcta tcacggaccc
      781 tggcagggcg acatcgacag ggtattcgac acgcaggcgt tctagatcat cacccgtgcg
      841 atgatcgcgt gatatcattg ccgtaaggga gagacctgcg aacgccaggg tttggaggtg
      901 ctcatgtcgc caagtttggg ccacattgcg ccgcccgttc tcggccttga aaatccccgc
      961 cgcgtaacca tatcgcgggc aagtcaccgt cccttctgca accctgcacg gatgggacgc
     1021 taatccgtcc cccaaaaggc ggatgttgtc ggcggcagag tctcctttct ctgtcgccga
     1081 tttcttttgc ctgaacaccc aggaattact tcaggttggc gagcgcgtcg ccgaagcctt
     1141 gcagcgcgac aggaatgccg atgccggcct ccggcgtatc gaagatgatg aacatcgcag
     1201 tcgtgccctt cttcatcttg gtgatgatgt cgtcctgaag gaccacctcg gcgacgcaac
     1261 cgcgcttgcc gcatttgagg aacggtgcgt tgccgacatc ctgaccgtca atcttgagcc
     1321 cgagccctgt gggcagcagg acgccgagcg gcacgaccac gcgcagaagc ttcttgtcct
     1381 cgccgatggc tttatagaat acgacggtca acccgacatt gggcctatcc tcggcggtga
     1441 cgctctgtac gagcgcgcat ttctcctcct tggctcctgg cggggtgcgg caagagacct
     1501 gccaagcccc gtgcgtctcg cgcaccacgc tgccgggacc cgtggcggcc ggcgccgcct
     1561 ccgaggcagc gccttgcgcc tgaacctgac cggcgagcgt gacactcaac aggaaggcaa
     1621 agccgagcag ggcaacggat ctcgcagcag gtaaaatggc ggacggacgc agcagtgcgg
     1681 cggcggcgct cgcgccgcgc cgcgcgcgct caacctcagt ggctcggctc gcaagcaaaa
     1741 gagattgtga gcggcggcgc agacaagcgg agatgcgcga catggaaaac cgccatcgcc
     1801 gagtattggg gccaaatttc gacgtgactc ggccggaaat cttgcccctt gcgccggagg
     1861 cccggcgtcg aggggggtgc ttatgccgca gcttggcctc gaattatgag tgttgccaat
     1921 ctatttcctt tatggtctga acaccgtaaa aggtcccaat ctggtgacgc aaatcagagg
     1981 ctgggccgca aaaaatgtac ggtgggggcg acgctcttta tcgctgttga tcttccgcgc
     2041 cgcccttgct tcgatcaagc gacgttggcg accgcgaggc cgccagcgct cctgaaattg
     2101 agaatgcgcc tgcccgccgt gggagagcgg caagaggcgg gaagcgacgt tagggagtgg
     2161 aaaggatggc aatctgccag cgaataacga cagcctttgg cgcggcagca agctttgccc
     2221 tgtgtggtgc ggcctatgcc aaggacgggc agccggtgcc ctggcagatc gattttcaac
     2281 ccgccgcctc gcccatcatg gagcagatcc actcgttcaa cacctatgtc accattatca
     2341 tcacgctgat cacggttttc gttctcgccc tcctcgtcta cgtgatggtg cggttcaacg
     2401 agcgccgcaa tccgcagccg tcccgcacca cgcacaatac cgtgctcgag attgcctgga
     2461 ccataattcc ggttctcatt ctcgttgcca ttgccattcc ctcgttccgg cttctgttcg
     2521 cccagtacga ctttcccaag gctgacctca acatcaccgc gaccggcagc cagtggtact
     2581 ggacctatga atatccggac catggcatca ccttcgattc gatcatggtg cccgaggcgg
     2641 acctgaaaga gggccagccc cgtctcctcg ccgttgaccg cgaggtcgtg gtgccggtga
     2701 acaagaacgt catcgtcggg ctgaaatcga acgacgtcat ccatgactgg gcgcttcccg
     2761 ccttcggcgt gaagctcgat gccgtgcccg gacgcttgca atcgacgtgg ttccgggccc
     2821 aacgcgaagg cgtgtattat ggccagtgtt ccgagctatg cggccgcaat cacgccttca
     2881 tgccgattgc ggttcgtgtc gtctcggagg ccgagttcgc cgactggctc ggcaagacca
     2941 aggcggctgc cgcgccgggc aacgcgggcc aggtggccgc gtcccaggag taaccgaaac
     3001 cgctggtgat cgcggcgaga aggctcgccg ccaacagaag taaccgatga cgaaatagga
     3061 cgaaagccat ggcacatccc gcccatgccg aagaggcccg ccacgaccac catcccacgg
     3121 gattggggcg ctggttcttc tcgaccaacc acaaggacat cggcacgatg tacctcgtcc
     3181 tggcgatgat cggaggcatc gtcggcggtc tcctgtcgat gggcatgcgc gccgagctca
     3241 tggagcccgg catgcagatt ttcggcaatc cggaggtgtt caacgtcttc gtgacggctc
     3301 acggtctgat catggtgttc ttcatggtca tgccggcgac catgggcggg ttcggcaact
     3361 ggatggtgcc gttgatgatc ggcgcccccg acatggcctt cccgcgtttg aacaatatct
     3421 ccttctggct gctcgccgcg gcgttcagct tgctggtgac gtccctgttc gtgcccggac
     3481 ctcccggcac caagggcgtc ggcggcggct ggaccgttta tgcaccgctc tccaccaagg
     3541 gcacgcccgg gccggcgatg gatttcgcca ttctgtcgct gcacctcgcc ggcgcctctt
     3601 cgatcctcgg cgccatcaac ttcatcacca ccatcttcaa catgcgcgcc ccgggcatga
     3661 ccctgcacaa gatgccgctc ttcgtctggt cggtgctggt cacggccttc ctcctgctcc
     3721 tgtcgctgcc cgttctcgcc ggcgccatca ccatgctcct gaccgaccgc aatttcggta
     3781 cggccttctt cgactccgcc catggcggcg atccgcttct atggcagcac ctgttctggt
     3841 tcttcggcca cccggaagtc tacatcctga tcattcccgg cttcggcatg gtgagccaga
     3901 tcgtctcgac cttctccaag aagcccgtgt tcggctatct cggcatggcc tatgccatgg
     3961 tcgccatcgg cgtgatcggc ttcatcgtct gggcgcacca catgtttgcc gccggcatca
     4021 gcgtcaacac gcaagcctat ttcgtgttcg ccaccatggt catcgccgtg cccaccggcg
     4081 tgaagatctt ctcgtggatc gccaccatgt ggggcggctc gctctctctc cgcgccccga
     4141 tgctgtgggc gctcggcttt atcttcctgt tcaccatcgg cggcgtcacc ggcgtggttt
     4201 tggccaatgc cggcgtcgac cgctcgttcc atgacaccta ttacgtggtc gcccacttcc
     4261 actacgtgct gtcgctcggc gcggtgttct cgctctttgg cgcctggtac tactggttcc
     4321 cgaagatgta cggctacatg tacagcgagg cgctcgccaa gctgcatttc tggctgatgt
     4381 tcatcggtgc caatctgaca ttcttcccgc aacatttcct cggactcgcc ggcatgccgc
     4441 gtcgttacgc cgactatccc gatgcgtttg cgggctggaa ctacatctcg tcgctcgggt
     4501 cctatctctc ctttgccggg ttcctggtct tcttgttcgg cattttctat gccttcgccc
     4561 gcaagcagca cgccgccgcc aatccgtggg gcgcaggtgc cacgacgctc gaatggaccg
     4621 tgccgtcgcc gcccccgttc cacaccttcg agacgctgcc gcgcatcaaa tgaagacgag
     4681 gacgtgcaag gtccggctgc tcggcttaag accgcaaatc atctgagtga gtgagcaatg
     4741 gccgacgccg caatttccgt agccaccggg acggccgata tcgatgccgg cggcgacgtc
     4801 gccgactatg tcgcgctgat gaagccacgg gtgatgttcc tcgtggtgtt cactgctctt
     4861 gtcggtctcg tcgcggcgcc cgtttccttg cacccggttc tcgccatcgc ttcgctcatc
     4921 tgtatcgcgg cgggagccgg cgccgccggc gcgctcaaca tgtggtacga cgccgacatc
     4981 gacgcgaaga tggcgcgcac ggctgcacgt cccgttccgc gcggtcgcgt cacggccgag
     5041 gaggcgcggt ccttcggcgc ggtcctcgcc gtgggctcgg tgatctgtct tggggtcatg
     5101 gccaactggc tttcggctgt gctgctcgcc gtcaccatcg gtttttacgt cgtcgtctat
     5161 acgatatggc tcaagcgcct gacgccgcag aacatcgtga tcggcggcgc cgccggcgcc
     5221 gtgccgccga tgatcggatg ggcggcggcg acgggcggca tcagcctcga atccttcgtg
     5281 ctgtttctca tcattttcgt ttggacaccg ccccatttct gggcgctcgc tttgttgagg
     5341 acgcacgact acgccaaggc cggcgtgccg atgctgccag tggttgccgg ccccgacgcg
     5401 acccgggtgc aaattctgat ctattctctc atgctcgcgc cgctcggcat gacgccggcc
     5461 atcatcggtc tcggcggcat cctctatgcg gttggagcct ccgtgcttgg ctcgctgttc
     5521 gtcgtcatgg ccctcacctg ttaccgggag cgggaagggg aggcggccga ccgcgccgcc
     5581 aagaacttgt tcgggtactc ggtgctctac ctctttttgc tgttcgccgc gctcctcgtc
     5641 gagcgggggt tcggcgttga cggcgggagc ctcctgtccc tttggacgtc gtgatgcaaa
     5701 atgctgagaa ggaacgccaa cgccgccaac gcatgcgatc catcgccatt gcctggatcc
     5761 ttgccgggct cgtggtgctg ttcttcatcg ttacgattgt caggcttggc ggcaacgtcg
     5821 ccaaccgccc gctttgagct taggccgctg tcatgagcga aacaggacag gcgagagacc
     5881 agaagccgaa acgccacggc gcggtcgcgc tcgcccttgt cggtctcgtc gccgtcatga
     5941 ccgggctctc cttcgccgcc gtgccgctct atcgcctgtt ctgccaggtg acgggttaca
     6001 gcggcgtgcc gcagcgggcg gaagccgcgc ccgagcgggg gctcgatcgc gccattcgcg
     6061 tccgcttcga cgccaatgtc gatcgtgccc tgccttggag gttcgcgccg gtcgagcgcg
     6121 tgatcgaggt caagatcggc gaaaccgcac tcgccttctt caaggctgcc aatcaatcga
     6181 aaactccggt gacgggaacc gccgtcttca acgtggtgcc ggaacgcgca gcgcgctact
     6241 tcaacaagat cgagtgcttc tgcttcaagg agcagacgct tgcccccggc gcgagcgtcg
     6301 agatgccggt gaccttcttc gtcgatccga aggtcgtcga agacgaggac accaagaaca
     6361 tctccgagat caccctgtct tatacctttt atcgcgccgg cgaggaggcg ggactagccg
     6421 cggccggcaa gtctggatca tgaccgaatt tgaggcgggg tttcgcctct gcgggtttgg
     6481 aggaacggga gcgaaccatg gctgaagcgc acgccaagca gcacgactat cacctcgtgg
     6541 atccgagccc atggccgatc gtgggcgctg tcggagcgct gacgctcgcc gtcggcacga
     6601 tcatgttctt catttcgaag aaggcgggta ccccgcagct ttggtacgtc gccccaggcc
     6661 tcgtgctcgt catcatcacc atgttcggct ggtggcgcga tgtcatcaag gaagcgaaaa
     6721 cgggctacga gacgcccgtc gtgcagctcc atctgcgcta cggcatgatc ctgttcatcg
     6781 cctccgaggt gatgttcttc gtcgcctggt tctgggccta tttcgacgcg gcgctctatc
     6841 ccgacgatgc cattacctat gcccgcaccc aggtgctggg cggccagtgg ccgcccatac
     6901 cgagcgccga gaccgacgtg cccggccttg gcagtttcag gcacactttc gatccatggg
     6961 ggctgccctt cgtcaacacg ctgatcctgc tcacctcggg cacgacagtc acgtgggcgc
     7021 accatgcgct tctgcacaac gaccgcagag gtctcgtcct gggtctcctc tgcaccgtgg
     7081 tgctcggcat cctgttcacc gcctgccaag cctatgagta catgcatgcc ggtttcggct
     7141 tcgccaacca catctacggt tcgaccttct tcatggcgac gggcttccat ggcttccatg
     7201 tcatcgtcgg cacgattttc ctcacggtgt gcctgatccg tgccatgcgc ggcgacttta
     7261 ccccgaagat gcatttcggt ttcgaggcgg cggcctggta ctggcacttc gtcgatgtgg
     7321 tttggctgtt cctcttcacc tgcatctatg tgtggggggc cagcagcgcc gccgggcatt
     7381 agcggcgtcc cgcgcgaaca aacaaagggg gcggtcgaaa gccgtcccct tttcgtttta
     7441 ggacatctcg atgacccgtg gcgaagcaag acccaatgtt ccgagcctcg ggtcgacgct
     7501 tgccgcgggg attgcgggcc gctgccccgc gtgccatcgc ggcaagctct attccggcta
     7561 tctcgcgctc gccccaagat gcgagaattg cggtctcgac tattctttcg ccgattccgg
     7621 cgacgggcct gcggtcttcg tcattctcgt caccgggttc gtggtaacgg gtctcgcgct
     7681 cctcgccgag atcttctacg ccccgcctta ttggctccac gccgtgctgt ggggctcgct
     7741 cgccatcctg ctgccgctcg tcctgctccg gtccttcaag ggcgctctga tcgcgctcca
     7801 gttcaggcac aaggccgaag agagcaagtt tgccgaatga ggcgggcgcc gatgtcgaag
     7861 gggcttctcg gcttcacggt gctgatgctc ttggcgcttt cggtgctcgt cggtctcggt
     7921 gtctggcagc tcgaacggct gcaatggaaa gagggattga tcgcgaagat cgaggcgcgg
     7981 accaaagccc agccgatcgg cctcaatgac gcagccgcca tggcggagga ggggcgcgat
     8041 ccgagctact accgggtgcg ggtcagcggg cgcttcgacc atgccaagga gcgatatctg
     8101 ttcgccgtct cagaggggag gggtggctgg catgtcatcg ccccgctcaa gacggttgat
     8161 ggggagacgg tcctgatcga tcgggggttc gtgcccgata gcctgaagaa tccctccgca
     8221 cgccccgagg gggaggttca agatatggtc accgtcacgg ggatcgtgcg ccttcccgag
     8281 acccggcata cgttcacccc ggaaaacgaa ccggaggcca atcgctggtt ctggcgtgat
     8341 cttgagagta tggcgcggtc gatgtttccg gccgaagccg tcaggctcgc gcccttcttc
     8401 ctcgaggccg ataaggggga ggtgccggga ggctggcccg aaggcggtca gacgcggctc
     8461 gaccttccca acaaccatct gcaatacgcc gtcacgtggt ttctgttggc ggcctgcctc
     8521 ctcgtcattt acggggcgta tgtgtggaag gctaaggcgc gccgatgggc gaccggcgaa
     8581 aaaggctaac tctgtggaga cggccgttat tgccgggaaa cgcagcgccc gctagggttc
     8641 cccgcgaaaa ccccctgccc gacgaggcct atcttgaagt tcatctcaac gcgcggtgaa
     8701 gcgccggcgc tttccttcga aggcgtgctt cttgccgctc tcgcccgcga cggcggcctc
     8761 tatctgcccg aggcatggcc cagtctcgag gccgaggcga tcgaaggcct cgcagggctc
     8821 gactatgccg acgccgcttc tcgggtgatg cggccctttc ttgccggcga tccgtgcctc
     8881 gccgatctcg atgcggtgct ggaggaggcc tatggcagct tccaccatcc cgccgttgcg
     8941 ccgctgcgcc agatcgcgcc gaacagcttc atcctcgagc tctttcacgg cccgacgctc
     9001 gccttcaagg acctcgccat gcaggttgtg gcgcggctga tgaaccgcgc gctgctccgc
     9061 aagggcgccc acgccacggt gatcggcgcg acgtcgggcg acaccggagc tgccgcgatc
     9121 gaggctttcc gtggtctcga ggcgatcgat attttcattc tgcatccgaa ggggcgcatc
     9181 agcgacgtgc agcgccgaca gatgacgaca gcttccgagg cgaacgttca caacatcgcc
     9241 gtcgaaggca cgttcgacga ctgccagacc atcgttaagg ggctgttcgg cgatctttcc
     9301 ttgcgcgacc ggctcgcgct gaccgggatc aactcgatca acttcgcccg catcctcgct
     9361 cagatcccct actacttcac cgccgcgatc agcctcggcg cgccccgccg cgccgtcgcc
     9421 ttcaccgtcc ccaccggaaa tttcggtgat attttcgcgg gttacgccgc cgcgcggatg
     9481 ggcttgccgc tcgcgcgcct cgttattgcc acgaacctca acgacagttt ggttcgcgct
     9541 ctgggttcgg gagtctacgc ccccatgagc gtgatcgcga cctctagccc cagcatggac
     9601 atccagcttg ccagcaattt cgagcgcctt ctgttcgagc ttgccggccg cgacgccgcg
     9661 cgagtgcggt cgctgatgga tgcgctgcgc aagaccggag ccttccgcct gaacgacgcc
     9721 gagctttcct ccttgcgcgg gcagttcagc gcgcatgcca tcggcgagcc tgagacggaa
     9781 atgaccattc gcagtctcgc ggaggagaca ggcgtgctgg ccgatccgca caccgccgtc
     9841 gccgtggcgg ccgcgcgcgc cgagagtggg caaggctcat ggccgatggt cgttatgtcc
     9901 acggcccacg ccgcgaagtt ccccgaggcg gtcgagcgcg cttcgggccg cgttcccgag
     9961 cagcccgagc gcctgagact gaggctcgga cagcacgagc gttgcgcggt cctccccaac
    10021 gactaccgcg cggtcgccgc tttcatcgag gcgaatgccc gcgctgaggg cagaaacagc
    10081 gcgcgagcgc ggcgccggac cggagtgagc gcgtgagcct gcaccagtca aaactcgcca
    10141 atggcctcac cgtggtcagc cacgccatgc ccgaagtgga gacggtgtcg ctcgggcttt
    10201 gggtaggggc gggcagccgg agcgaggcgc ccggcgagca tggcgtcgct catttcctcg
    10261 agcatatggc gttcaagggc acggcgcgcc gaagcgcgca agatatcgcc gaagagatcg
    10321 aggccgtggg cggcgagctg aatgccgcaa ccggtgtcga ttccaccgct tattatgcgc
    10381 acgttctgcg caaggacatg ccgctcgcgc tcgacattct gagcgatatc gtcgtcgctc
    10441 cgcgcttcga caagatcgag cttgcgcgcg agcgcgacgt gatcctgcaa gagatcgccg
    10501 ccgcgatgga ttcacccgat gacatcgctt tcgacctcgt ccaggaggcg gcctaccccg
    10561 gccaggctct cggcagaccg attctcggca ccgcagagag cgtgtcccgc gtcaagcgcg
    10621 atcacctcgg cgcttatctt tccgcgcatt atcacgggcc aaacatggtg cttgccgcgg
    10681 cgggcgccat cgaccatggc gcgctcctcg ccgaggccga gcgcgggctc gggagtttcg
    10741 atcctatgcc tgcggcgcct ccggaagctg ccacctatac cggcggcatc agacgctccg
    10801 acaagacttt cgagcaaacc catgtcgtgc tcgccttcga gggtccgcac taccgccacc
    10861 ccgattattt cacggcacag atttgcgccg gagcccttgg cggcggaatg tcgtcgcgcc
    10921 tcttccagga ggttcgcgag cggcgtggcc tctgctatgc catctatgct tttgccagcg
    10981 gaatttccga cagcggcatg ttgaccatcc atgcggcggg ctcctccgac cgagcccata
    11041 acctctttgc cgtgatccgc gacgagttgg tcaaggccgc cgagaagggt ttccgcgcgg
    11101 acgaggtcgc ccgggtcaag gcccagctca agatggggct tctgacaggc cttgagagct
    11161 cgagcgcccg cgccgagcag ctcgctcgcc agatcctgat ccatggccgg ccgctttcca
    11221 gcgaggagct gatcgaaaag gtggaccggg tcgaggccca cgacatgcaa gccttgattg
    11281 agcgtatgct cgcctctccg gtgagcctcg ccacggttgg gccaattctt catgttgcaa
    11341 gattcgaccg agtgacggaa aaattcgccg ttccctcgac caaggccgct tgagggggtt
    11401 tatagctcat ggctttactg cgagccgtct cgaccagcga tctcggaccg gtcatcgagt
    11461 ccgatgcggt cacgttgcgg acgccgcaaa tgtcggacta cccggaatgg gccgagctta
    11521 gggctcagag ccgggaattc ttgagcccct gggagccgct ctgggcggcc gacgagctat
    11581 cccgcgcctc gttccgccgg cgcgtccggc attatttgcg cgatcttcgc gaggacgtgg
    11641 gctacgcgct tttcatcttc gccgtcccga ccggcgcgct ggtcggcggg ctcacgctct
    11701 gcaacgtgcg ccgcggcgtg acccaatcct gcacgctcgg ctattggatt ggagccaagt
    11761 acgccaatca gggctatatg acggcagccg tccgcgccgt cgtccccttc gtgttcgatt
    11821 cgctcgaatt gcatcggctg gaagcggcat gcctcccgag caatacggcc tcgatcaggc
    11881 tcctcgagag aacgggcttt aggcgcgaag ggctggcgcg gcgctatttg cgaatcaacg
    11941 gcgtatggca agatcatctg ctctacgcac tgctcgacac tgacacgtgc cgctagcggg
    12001 cgatcgtcag gcaacaacgg gggaggaaag gcggagtgag cgcctcggta aggggtaaga
    12061 cggcgcctgt gcggcgcttt gtcggcccga tcggcgccgt cctctgcgcc gtcgccgccc
    12121 ttctcgtgtg ggcgccgccg gccctggcta tcgaagcaat ccctgtcgag cccaatcagg
    12181 acaagatcga cattaccgcg aagggcgagc tctatgaggg gcgaggcgat cggcttcaga
    12241 tcgagacggc gccgggcccc gacggatata tcggccgcat ggccgtttcg gcctcgacac
    12301 ccggcaccaa tccggcctgg ctcgtcttcg ctctctccaa tccgacttca gagcgcatcg
    12361 tgcggtggct ggtggcgccg cgctatacgc tggccgactc gcgcgttctc tggccgatcc
    12421 tcgactcccc gcgcatcgcc gccatcaccc cttcgctcgg gttccgcccc gaaccgttga
    12481 agagcgatcg cgccgacatg ttccgcttga gcctggagcc ggggcagacc atcacctttg
    12541 ccgccgaact gagctcgcag caagtcccgc tgctcaatct gtgggacccc aacacctatc
    12601 aagcaaagca gcaggatcgc atgctgttca acggcgtgct gctcggcatc accgggctgc
    12661 tcgccatttt cctcaccgcc atcttcgccg ccaatcaccg cgccatcttc cctgctacgg
    12721 ccttgattgc ctgggccgct cttgcctatt tctgcgtcga tttcggcttt tggaacaagc
    12781 tcttccccgt cggtcccgac cgcacggcgc tctatcgcgc ggcagccgaa gcggggcttg
    12841 ccgcgagcct cgtgctcttt ctttacgcat tcttgcgcat cgggctttgg catggctgga
    12901 tcaggacgtt gttctggggc tggatcgcgt tccagctctc gctcgtcttt cttgccatcg
    12961 tggacccggc ctcggcgtcg ggcctcgcgc gcgtgtcgct cgtcgccatc gccggcgtag
    13021 gtgcggcgct tatcctctat ttcgcgctgc gcggccagga tcgtgcattg tcgctcattc
    13081 cgagctggat gctgctgatc gtctggctgt tcggcgccgc catgatcgtg ctcggccacc
    13141 tgtcgggaga ccttgtcgtt tcgggcctca tcgccggact ggtgttgctg ctggtgctgc
    13201 tcggcttcac cgtgacgcaa ttcgccttcc gctcaggcgc cacgcccttg agccttgggc
    13261 cgcccagcct cgtgcagttg aagtcgctcg ccgttgacgg ctccggcgcc tacgtcttcc
    13321 agtggcacgc cggacgggac gagatcaccg tggcgaacga ggtggagcac gagcttgggc
    13381 tcgagcccgg cgcgctatcc gtcgcggtgg acgactttct gcaatacgtg cacaacgccg
    13441 atcgcgagcg tgtccgcctc atgctgtggt cgctccagga gaagcagggc ggggacattc
    13501 aggtcgagtt tcgcctgcgg cggcatgacg gcgcctatct ctggtacgag ctgcgcgcgc
    13561 acgcggcgcc gagcgagaac ccaaggctgc tccgctgcac cggactgatg cgcgacgtca
    13621 ccaacctgaa gcgttcccac gagcggctga tccacgacgc cgtgcatgac agcctgaccg
    13681 gccttcccaa ccgcgagctg ttcatggatc gcctgcaagg cgcgatcacc cgcgcccgcg
    13741 agggccaagc caatcgcccc accgtgctcg tcatcgacat cgaccgcttc aagaacgtga
    13801 ataagagctt cggcctcgtg atcggcgaca gcatgctgct cacgctcggc cgccggttga
    13861 gccgccacct caatccgcag gatacgcttg cgcggcttgg cggcgatcaa ttcgccatcc
    13921 tgctaatctc tgatgcggat cctcgcaacg tggcgacgct cgccgagcgc gtgcggcgcg
    13981 cgctccgcac gccgatgaag atcagcgcca aggagatcat cctcaccgcc tcgatcggca
    14041 ttgtcgtcca tgacggcagc caggagacgg cgcaggagat gctcagcgag ggtgagcttg
    14101 ccatgctcag ggccaagcgc gccggcgccg accgtatcga gattttcaat cccgcgatgc
    14161 gggaggagga cgaaagcaag cttccgctcg agagcgatct gcgcaaggcg ctcgagcggc
    14221 agcagatcgc catactctat cagcccatca cccggcttgc gtccaaccag ctcgccggtt
    14281 tcgaggcgct catgcgctgg gaccatccga cgcgcgggcg gctcggcgcg gaggatttca
    14341 tcccgattgc cgaggaatcg ggactgatcg tcgagctcgg aagctacgtg ctcagccagg
    14401 ccctcgaaga agcgaggagc tggcacaagg cgctgccgcg cgaccgcgat ccgctcttcg
    14461 tcagcgtcaa tgtctccagc cgccaactgt tccgccagga catggtccag gagatcaggc
    14521 tgatgctggc gcgcgaggcc gtgcccaagg ggacgctgaa gctcgaaatt accgagtcgc
    14581 tggtcatgga aaatcccgaa cgcgccgtcg agattctcac ctggctcaag accttcggcg
    14641 caagcctcgc gctcgacgat ttcggcaccg gctacagctc gctcagctat ttgcaccgct
    14701 tcccgtgcga caccatcaag gtggaccggt cgctcgtccg caatagcggg atcgacggct
    14761 cgagcccgct catccttcgt tcggtcatcg cgctcgcgca cgagcttggc aaggaggtgg
    14821 tggcggaggg cgtcgagacg caggccgatg cgagctatct gcgctcgatc ggctgcgaat
    14881 acggccaggg ctactattac ggcgagccga tgccggccaa ggacgtcgcc aacttgctcg
    14941 cggcgcttgc cagtcatcgc aagcgccgcg agcgcgagcg cgccagggcc cccgcgcagc
    15001 cgccgcaagg attggacgtg caggtcgcag gcccgggtcc gctgcccgct ctgcaagggc
    15061 ccggcacgct gccatccacg tcctagcaac ccggcgcgcc gtgcgctcaa gcagcggagc
    15121 ggtagcgcaa aaaccgagcg gcgccccgac ctccccacga gggggaggtt agaagagagc
    15181 ctctctcacg gaaaaggggt tcaggttagg cgtggcctga atcgttgacg aggcggatcg
    15241 gatcgatccc gagcttgccg agcgcgcgta aatatttgcg gtcgacgtcg ggatcgaaga
    15301 tgagcccagg gtcggcgggg caattgagcc agccattggc gtggatctcg ctttcgagct
    15361 gccccggcgc ccaccccgca tagccgagcg caagcagcgc cttgtccggt ccggtccctt
    15421 tggcgatgtc gcgcagaatg tcgatggtgg cggtgaggca gacgctctcg tcgatcggca
    15481 aggtgctttc ggccttgaaa tagtcggcgc tatgcagcac gaagccgcgc ccggtctcga
    15541 ccgggccgcc gagatgcacg gccatggcgt gaagcccggc gggaaggctg atacggtctt
    15601 gcgtcggcac gatgttgagc tgctccagga gctcggtgaa gctgatattg ggcgcgcgct
    15661 gattgatgat gatgcccatg gcgccgtcgg ccgaatgggc acagagatag atcaccgaac
    15721 gcgcaaagcg cggatcgccc atggccggca tggcgatgag caattggccg tcgagataac
    15781 cggtgccctt cggacgattc ggtgcgcttc gatctggcat ggcaccacag tacaggagtt
    15841 taggtgctcg attaagaggg aagcgaggcg ttttcgccac atgcgctcac aaaagagtga
    15901 ggcctttcgt ttctgttgaa gtcgcgcagc acgtcccctc ccgctaaagt cacgagccca
    15961 atcacgcgcc ttaaaggggt cggcatgcga tctttgttgt tgccacgaag actgttcgtc
    16021 ccgctcctga ccgttgctgc gatcttggcc cttgacgcag ccgcgctcgg cggggcgccg
    16081 agcagctcgc cttggacggc ctccaccaat tccaaggtga ggctcgtgtc gggcacggtc
    16141 gttaacggaa gcggcactgc gctcgccgcc ggcgttcaac ttcgcatgga acccggctgg
    16201 aaaacctatt ggcggaaccc cggcgattca ggcgtgccgc cgagcttcga ttggaccgga
    16261 tcgaagaatc tgaaaggggc cgaagtgctt tatcccgcgc cgcaccgctt tgccgatgcc
    16321 ggcgggacgg cgatcggcta cggcgaagac gtgctgttcc cggtcaagct cacgcccgag
    16381 cgtgacggcg agccaatcga agtcaagctc gctttcttct atggcctgtg caaggacctt
    16441 tgtatcccca atgaggtgaa cttgagcctt gcgctccctt ccgatgagga gaagggcgaa
    16501 gcgctcatcc tcgatgccgc cttggcgcag gtgccgaagc ctgcccgttc gggcctcttg
    16561 ccggaagtga acggtgtcga agcgaagctt gacggagacg cgcccggtct cgtggtcgat
    16621 gcggtcttcc ctccgggcgc gaccgggacc gatcttttca tcgatggcgg cgatgtcttc
    16681 gtgccggttc cggcaccgct tggcgcgctg gtcgacggga agcagcgttt tgccgtcacc
    16741 tttgcttccc cgtccgaggc ggccgcgatc aagggcaaga ccttgaggct gacgctcgtc
    16801 tccgaccagg gttcgaccga gacgatgtgg aaggcggagt agcgcccctc acttgaacgg
    16861 cgccttggcc gagaagcgcc gagccgctat ggtcggcttg agccccgaca cgaaggaaga
    16921 gtcaaggaga gtatcgatga ccattgctgt aggagaccgc ctgcctgacg ccacgttcag
    16981 gttcttgggc cccgatggaa tcacatcgac gacgacgcgc gacgtattcg gcggcaagaa
    17041 ggtggtgctg tttgcggtgc cgggggcctt taccccgacc tgtcacttga aacacctccc
    17101 gggcttcatc gagagggccg ccgaattcaa ggagcacggc gtcgacaccg tggcttgcgt
    17161 cgcggtgaac gatcctttcg ttctgggcgc atgggaggaa aagtccggcg gcaaaggcaa
    17221 ggtgcagttc ttgtccgacg gcaacgccga gttcaccaag aagatcggca tggatttcga
    17281 cggaagcggg atcgggctcg gcacccgctc gaagcgctac gcggcgctgg tcgacgacgg
    17341 cgtggtgaag attctgaatg tcgaggagtc gccgggcgtg gccgccgaga gcagcgccga
    17401 caagatcctc gaagaccttt agcggaggaa ggggcttcac tccggcctga ggcggcggcc
    17461 ttcgaggaac ggcgccatgc cttcgcgcgc aagctcgtcc gcgcgctcat tggcgtcatg
    17521 gccgacatgg cccttgaccc aatgccagcg gaccgtatgg cgcgacgcgg ccttctccag
    17581 ccgctcccac aactccgcgt tcttgaccgg cttccgatcc gccgtacgcc agccgttctt
    17641 tttccagctt ttgatccagc tcgtgatgcc gccgcggaga tagttcgagt cggtatagag
    17701 atcgacctcc gacgccgcct tcaacgattc gagcgccgtg atcgcagcca tcagctccat
    17761 gcggttattg gtggtcaggg cctcgccgcc catgagctcc ttgcgatgcg cccccgattg
    17821 gaggatcacg ccccagccgc ccggtcccgg attgccggaa caggcgccgt cggtatggat
    17881 caccaccttg ggccgcgccc ccatgctcag cgctccttga gcccgtaatc gcgcgtcgac
    17941 ttcacccgct gatggaatct cagcttgcgc agatattcgc cggggtcctt ggtcttcacc
    18001 agcgcgttcg cgtccgtgtt gatgagatcg taggtgcggg tcaagagaaa gcgaagcgcc
    18061 gccccgcgtg caagcgtggg cagacgatcg agttccgcgc gggtcaagcg gcgcaccgcc
    18121 tcgtaagcct cgagcagcgc gcgggccttg gtgacattga agctgccgtc ggtctcgaag
    18181 caccaggcgt tgaggcagac ggcgacgtcg taggcgagca tgtcgttaca ggcgaaatag
    18241 aaatcgatca ggcccgagat cttgtcgccg aggaagaaaa cgttgtcggg gaacagatcg
    18301 gcatgaatga ccccttcggg cagatccttt ggccagtttg cttcgagatg ccgaagctcc
    18361 ttttcgatga cgcctgaaag tccttgtgcg accctctcgg caccgccgcc gatcatcgcg
    18421 aacaaggctt gccagccggg gagcgacagg ctgtttgccc gcgccatggg aaagtctcgg
    18481 cccgccaggt gaaagcgcgc taacgcgtcc ccgaggcctg cgcaatgctc gatgccgggc
    18541 ctgcgcaccc acagcccatc gagaaaggtg atgagcgcgg cgggacgccc cgcgagcacc
    18601 ttcagcgttt ggccgtcgcg atccttcacc ggaagcggac aggtgatgcc gcgcgcggcg
    18661 aggtgctgca tcagggcgag gaaatacggg agatcctcgc ggctcacccg tttctcgtag
    18721 agcgtgagga tgtagcgccc gctctcggta tgaaccaggt agttggagtt ctccacgccc
    18781 tcggcgatgc ccttgaagga ggtgagggcg ccgatggcgt agcttccaat gaaggcctcg
    18841 agctcttcgt cggaaacgtc ggtgtagacg gccatctaga gcgccggcat ctctctgagc
    18901 tcgcgcggaa gcttgaaagc gatgctttct tccgccgtgg tcacggtctc gaccgtcgtc
    18961 tcgaagcgcg ccttgaaggc gtcgatgatc tcgtcaacca gcacttcggg cgccgaagct
    19021 cccgccgtga ccccgaggcg cgagatcccg ttgaacatgg cccagtcgat ctcggccgcg
    19081 cgctggatca agacggcggc ggggcagccg gcccgctctg ccacctcgac caaccggatt
    19141 gagttcgagc tgttgggcga gcccaccacg atcatgcgct cgcaagccgg cgcgatgcgt
    19201 ttcaccgcct cctggcgatt ggtcgtggcg tagcagatat cgtccttgtg cgacgtgacc
    19261 atgcgcggaa agcgcgcctt gagcgcgttc aggatctcct tggtgtcgtc gaccgagagc
    19321 gtagtctggg tgatcaccgc gagcctgtcg gggtcgcgcg gaacgaaggc ctgcgcatcc
    19381 tccaccgtct cgatcaggat cacggcgccg tccggcaact ggccggtggt gccgacgacc
    19441 tccggatggc cgtcatggcc gatcagcagg atgtcgcgcc cctcgcggaa ttgacgctca
    19501 gcctcgacat gcaccttgga gacgagcggg caggtggcat cgagaaagaa gagattgcgg
    19561 gcgcgcgccg ccgccggcac tgccttcggt acgccatgcg ccgagaagac gatcggcgcg
    19621 ccgttatccg ggatttcgtc caattcttcg acgaagatcg cgcccttggc cttgaggctc
    19681 tcgaccacat agtggttatg gacgatttca tggcgcacat agacgggcgc cccgtatttt
    19741 gccagtgcaa gctcgacgat ctggatcgcg cggtcgacac cggcgcagaa gccgcggggc
    19801 gcggcgagat agatggtgag cgaaggacgg tcggagtgcc gccccttggc ctttgtgata
    19861 ccggtcatcg tcgtcccgga agatgcgtca gtctgacttc aggcgagctc agtctggcct
    19921 tgtcgcgcct cccggctggg gctagtttgc gcgcgaaaga aagaccggca acccgcgata
    19981 tttcagagca cttgccagat cgagcggcat atcaggcggg tgggcctggc gtgtcaagcc
    20041 gaggacgggg tcaggtgagg cgtaccgacg cgcgatcatg gctcagccga tgcgcccttg
    20101 ccgggctcgt cgccgtcccg ctcttcgtct ccggctgctc cgtcccgcgg ttttccaacc
    20161 ccttcggctc cgacgatgac gacacgtccg ctgtcccgca aacgagcatg ctcgagaacg
    20221 ccaaggtcga cgtggccagc ctgccgagcg ggtacagtgc caattgcccg caaatggtcg
    20281 cctggccgcg cgaccggatg ctcaccgtct accagcccga tcatattggg gataacaacg
    20341 ccgtcgtgca tcgcggcgag atcaccaaga tgtcgcggga gtgccagctc cacgccgacc
    20401 gcgtggtcgt caaatatggc tttgcaggcc gcgtgctgct cgggccgaag ggcggtccgg
    20461 gtaccgtcag tttcggtgtc agcctccgtg tggcaggcgt ccagcagaac acgctggcga
    20521 ccgacaagat gaaggtgacg accacgatcc cgcctggggg cccggccggc tacttctcca
    20581 tggtcgggca aatcgccttt cccatcgtgg tcgggacaag gcctcaggat tacaagatct
    20641 tcctcgcttt cgaccgcagc gttgcggggg cgggctaaga ttttggtgta aggcctcatt
    20701 cccggccgcc gggtcgggca ggaggtgccc tcctgttttc atggtcatgc caaaatttga
    20761 tcgagccgcc ctgctacgat tctcggagtt ctgcgttatg cggcaggctt cgcgtgtgcg
    20821 tcagggggat aagccggtga gggaccttta tcgaattctt gggattgggc gggaggctgc
    20881 ccgggacgag atccacaaag cctacaggcg caaggccaaa gcctcgcacc ccgacagcgg
    20941 cgggtcggtc ggtgccttcg gcgagcttgc gactgcctat gccgttctct cgaacccgag
    21001 ctgccgcgaa cgctacgaca cgaccggcca ggtcgagcct cctcgtccca acaatttcga
    21061 cgcaagcgcg gtggaggtca tcgcccagaa gctcggcctg atcatccatg ccgaacagga
    21121 catgaccgac atggatatcg gcgccctgat cgaggccgcg atcggcgagg acatggccga
    21181 gcgccgggcg aatattgcaa gccaaaggcg cgcgatcggc cgggccgagc gcttgcggtc
    21241 taggatcaag cgcaaagcga acggcaatga caacatgctg gcgcgggtgc tcgactggca
    21301 cgaatcctcg acccggatgc agatcgacaa gaatgaagcg gccgtcgcct ccatggagcg
    21361 cgcgcttgag attctgaagg actactcgtt caccgaggat cttccgcgtg gggcggccgg
    21421 cgacgaggtg tcgctcgcct tgaacgacgc gctccactcg ctcgatcagc tggccgccgt
    21481 gctcaagacc gggcccgcct ggtgcgatct cgatatcgcg cggccttcgg cgagcggcta
    21541 gaggcgtcgt catcctgagg ttcgcggcct cctcaggatg acggggaatt taggaaagac
    21601 taattcagcc ggcccttgag cgcgtcgatg ccctgcttga cgagcttgtc ggcggcttcg
    21661 ggcgtgagct tctctgcgat cacggttctt gcggcggcga tcgcggcatc ggcggcggcg
    21721 gcacgcacct cgcgcagcgc gtccgcctcg gcgcgcgcga tcttgtcctc tgcgagcttc
    21781 atgcgccgct cgaaatgctc cgtcatcgag cggtgtgtct cggccgcgag gatctcggcc
    21841 tccttggcgg caaggtcgat gatcgcttgc gcctccttgg ccgcatcgcc ctctttccgg
    21901 cggtagtcgg ccagcacctt ctcggcctct tcgcgcaggc gccttgcctc gtcgagctct
    21961 ttggcaatgg cggcggccct cgcatcgagc acggaggcga gcttcttgtg cacgccgaaa
    22021 tagagcaggg ccccgacaaa gaggaaaaac gaaaccgcga cccagaattc gggcgtcatc
    22081 aggacttcca tggtcaccct ttccgcgccg ttccgaccgc cttatcgatt tccgccttgg
    22141 ccggcgcgac ccctatgagc cggcgcacga tatcggccgc gacatcggcc gcgaccgtgt
    22201 tcacgtcctt catcgccttg gctttggccg cgccaatgcg cgcctcggcc tcggcgcctc
    22261 tctcggcgag gtcgccgtcg agacgcgcgc gctctgccgc cgtctctgct ttgagcttgt
    22321 cgcgtccctg ctcgatgatg gcgtgagcct tctgccttgc ctcggccagc gcctgctcgt
    22381 aggccgcgat cgcctcctcg gtctcgcgcc ggagcttgtc ggcggtggcg agatccttgg
    22441 cgatacgggc ggcacgcgcc tcgatcaccg agccgatacg cggcagcgcc acatacacca
    22501 tcagcagata gaggatgccg aaggtgatga ggagccacag gagctgcggg ccccaatcga
    22561 gtgggttgag ttgcggcatg gtcgcctgtc tccgctcttt aagcggagac tccccgtgac
    22621 cctagaacac gaacaggatg atcagcgcca caacgagccc gaacagtccg gtggcttcgg
    22681 cgagagcgaa gccgagcagc aggttggcga actgcgacgg cgctgccgac ggattgcgca
    22741 gagccccttg caggtagttg ccgaagatcg tgccgatgcc gacgccggcg ccgacgagcg
    22801 cgatcgaggc gaggcccgcg ccgatcaatt ttgctgctgc tggatccatg tcttctctcc
    22861 tttgcaagcg atgtcgtgtc gggaacagat ggctagtgat gcatatgaag tgcgtcgttg
    22921 agatagatgc aggtcaggat ggcgaacaca taggcctgga ggaacgcgac caggatctcg
    22981 aggccggtga acgccaccat gaaagcgagc ggcgcccacc ctccgagaaa gccaagcgcc
    23041 acgacgaagg cgccgaacac tttcagcata gtgtgccccg ccatcatgtt ggcgaacagc
    23101 cgcacagaga gcgagagcgg gcgcgtgaga taggagatca cctcgatgac cacgagcagc
    23161 ggcagcatga cgaaaggcac gccgtcgggc acgaagaatt tgaggaactt caccccgtgc
    23221 ttgacgaagc cgatcagtgt cacgctgaga aagatgaagg cggcgagcgc gaaggtgacg
    23281 atgatgtggc tcgtcacggt gaaggaataa ggcagcatgc cgagcatgtt cagcgttagc
    23341 acgaacatga acagcgtgaa gatgaagggg aaatatttca tccccgcggt gccgacattg
    23401 tcgcgcacca tattggccac gaactcatag gatagctcgg ccacggactg gagccgggtc
    23461 ggcacgagcg cgcgtccgcg catggcatag acggtaaaga gggtgatgag cgtggcggcg
    23521 atcaccatga agagcgccga gttggtgaac gaggcgtcat agccgaacag ttcgagcggg
    23581 atgagccgct tgatctcgaa ctgatgcatg ggatcgggca tgccgccggc gctgccgcca
    23641 tgccctgcgt cctctgcggc gaaggctgtc ccgatcactg caaaatgccc ctaatcgtca
    23701 tcgtcgtcat tcacgctcgg caaatccttg cccgaaggcg cctccgcctg catgcgcttg
    23761 gcggtccgca ccacgttgag gatccccgcg gcggcgccca gtccaaggaa cacaaccatg
    23821 aggaagggcg ctgtgccgaa caggcggtcc agccaccagc cgatgaaaaa cccgaccgca
    23881 acgcccgcga ccagctcgac cccaagcttg agcgcctggc cgagcgcggc cccctggctc
    23941 ggcggtgcgt cggcgtgaac cggcccgtgc cgggactgag cttcgtcgag cttttcgccc
    24001 agggcatcga gccgctgccg aatttctccg gtgtcgcggc ggacttctct gcggccggac
    24061 tgatttttag ttccggccat cggcacgggc tcaatttccg ggagggcctt taggaccggc
    24121 tggtcccaag cttggccggc actttagttg gggatttcag gctgtcaagg aagtcaaacg
    24181 ggtgggtggc aagcctgact tcgccgaggg agcatggatg cagggagcgc gccgggtggg
    24241 gggcgtcagt gcgaggcgag ttgtgctaaa ctgcgctgaa cgcggacgga aaacatgaac
    24301 gccaagacca aagctttgag cgaagaggcc cgcaaactca gtcctgacga gcggatcgcg
    24361 ctgatcgaag accttcagcg cagcctcgtg cccaccgatc ctgaaatcga tcgagcctgg
    24421 gccgaggaag ctcgcgaccg gctcaacgcc tatcgtcgtg gagaaatcga agtgatgacc
    24481 ctcgaggacg tcatcgcgag cctcacaaag aggtgacggt ccgcttcctc aaagtcgccc
    24541 aagttgagct acgtgaagcc gttcgttact acgaatcaca ggccgctggc ctaggagccg
    24601 acttcttgct ggaagtgact gcggctgtcg aacggataac ggaatttccg gtagcgtggc
    24661 agacggtgga tgtggagctg cgccggtgcc aactcggcag atttccctat gccttggttt
    24721 atgccgagga cggtgatggc gtcctaattg ttgctgtctc gcaccttcat cggcgccccg
    24781 agaattggca gcgccggctt cgggagacgg aataactagc ttgcgtcgcg gaggcgctcg
    24841 gcatgctcga gatcgaccga gacgagctgg ctcacgccgc gctcggccat ggtcacgccg
    24901 aacagtcggc tcatgcgcga catggtgagc gggtggtgcg tgatgatgag gaagcgtgta
    24961 tcggtgtctt tcgccatctc ttccatcagc aggcagaagc gctcgacatt ggcgtcgtcg
    25021 agcggcgcgt ccacctcatc gagcacgcag atcggcgagg gattggtgag gaacacggcg
    25081 aagatcagcg caagcgcggt caaggccttc tcgccgcccg aaagcagcgt cagcgcttgc
    25141 agccgctttc ccggcgggca ggcgaagatt tcgagtcccg aatcgagcgg atcgtcggaa
    25201 tcgacgagct tcagctcggc cgagccgccg ccgaacagcg tcttgaacag ccgcccgaaa
    25261 tgactgttca ccgcattgaa ggcttcgagc agccgcttgc ggccttcgcg gttcagattg
    25321 gcgatccctt gccggagccg caggatggcc gcctcgacgt cggccttctc gcgctccatc
    25381 tcttcgagct gcgcggcaag ctgcgcctct tcctcttcgg ccatgagatt gacgccgccg
    25441 agccgctcgc gatcgcccct gagcttgaga agtcgcgcct cgacctcggc aagcggggga
    25501 aggggcgcgc cctccttcac gcccgcgagc gcgaggcagg cttccggagc gcagtcgagc
    25561 tgatcgcgaa tggtgcgcgc ggcatcgctg cgtcgctcgc gcgccgcttc gagccgtgcc
    25621 tcgttccggg cgcgcgcctc gcgcgctccc gcgagccgct cctgaacctc gcgtaacgcc
    25681 ttctcgcgct ctttgagcgc cgtctcggca agggcaagct cgtcggcggc cttcgacctc
    25741 tcgtgctctg caccggcgat ggcgtcgaac agcttcatcc ggcgctggtc gatctcggcg
    25801 ggaagattgg cgagcgcggc aagatcagcg ctcgtttcct cctcgcgcgc gcgcaaggtg
    25861 agaatctgct cgcgcgcatt ggcgatgcgc ttccgccaca gctcttcttc ctcgccgatc
    25921 gcctccatgc gctcttgccg gagcctgagc tcgcgctcga agccttcgag cgcggcgtcg
    25981 gcgcgggcag cggcctggcg gccctgatcg gcccgcgcca gcgccgcttg gagcgctgtc
    26041 tcaagtcctg agagagaggc aagctcaagc agggcggacg cggcgcgcgc gctcttcgct
    26101 tgcgcctcgt cgaacgccgc ccgcgtgcgg cttagcgcct ccgtgcgtgc tccgagttcc
    26161 ttgctcgccg cttgcgccgc gtgttcggaa gcggcgatcg cgtcacgcgc gcggtcgagc
    26221 tgggcatgtg cgtccttggc ctcctggcgc aaggccttgg tcgcaagcgc tgcgcgctcc
    26281 gcgcgctcag atgccgcccg aagcgcggat tcggtcgcgg cggcgcgcgc ttgcgccttc
    26341 gcggcttcgc catgaagcgc cttcaggcgc cgccgctcca aaagccgcgc tccggcggcg
    26401 ctgtcggtcc cggccttgag gctgtatccg tcccagcgcc acaaatcgcc gtccttcgac
    26461 acgagcctct ggcctggcgc gaggctggcc tggagttttc ggccgcgcgc cgcatcgacc
    26521 acgccgatct gtcggagccg ccgggccagg acctcagggc ccttcacgaa tttgctgagc
    26581 ggggtcgctc cctccggcag ctccggatcg ccgcccgaag gcgccgtcag ccgccaatgg
    26641 gcgggcgcct tctcgtcgct ggccgcgtcg agatcgtcgc caagcgcggc accaagcgcc
    26701 tgctcgtagc ctgacgtgat tttgatctcc gcgacgatcg gcgaccagtc atgggccggc
    26761 gcgagcagct tgatgagcgt ggcaagctcg gtctccagct cctggcttgc gagcttcgcc
    26821 gcggaggccg tcgcgcgcgc ctcggcttcg ctcagccgtg ccgcttcctc ctcggcctcg
    26881 gcggcttgga gctcgacttc gagcctttgc acgttggcgg aaaattcggc caatccggca
    26941 ttgagagctt gcagtttccc gccgctttcg ctttcagcct ggcgcgtcgc cagttccgcc
    27001 tcgatctccg cggcctgggc ttcgagcttg gccatgcggg ccagaagttc gactttttcg
    27061 ccctcgtgct tgccgcgctc ggcgcgcgcc tccgcgagcg ccgtggtcgc ctcgcgcaag
    27121 gtcctctcgg cggcgtcgag cgcatccttc gccttggcgg aggcggcgcg cagttgcggc
    27181 tcttcatgag cgcgcgagct tgccgcttca agccggcgct tctcctcgcc aagacggcgc
    27241 aacatgccgt cggcctcggc gatatgttcc tcctcgcggg cgagatcgcg cgaggcctgc
    27301 gcgagccttg tttcaagctc ggcttggcgc gccttggcgc gcgcttcctc gcgctcgagc
    27361 gcgtcgcgct cgacgctcaa gcgatgcagc actgccgcgc gcgcggcctc ctcgtcgcgc
    27421 agaggctgga gcttctccgc ggcctccgcc tgctggcgaa gcgcctcgcc ctcggccttg
    27481 gtctcctgcg cgacggcggc gagcgcctgc tggaacgcgg cctcctcctc atcgacatgg
    27541 gcgcaggcgc cgacccaatt gagatgatgc tgaagcgcct cggcctcgcg gatcttgccc
    27601 gaaacctcgc ggtagcgctg cgcctggcgg gcctggcgct tcagcgcctg caactgcgag
    27661 gcaagctgcc cgatcaagtc cgacaggcgc gtgagatttc cttcggtcgc ggcgagcctg
    27721 agctcggcct cgtgcctccg gctgtgcagg ccggcaatgc cggcggcgtc ctcgatcacg
    27781 cggcggcgct cctgcggcgc cgcgttgacg atctcgccga tccggccctg gcgcacgagc
    27841 gcgggcgacc gcgcaccggt tgccgcgtcc tcgaacaaga gccgcacgtc gcgggcgcgc
    27901 acgtcgcggc cgttgacctt gtaggccgag ccagcctcgc gctcgatccg ccgcgtgact
    27961 tcgagcgcat cgctgtcgtt gaactccgcc ggggctttgc gcgcgctgtt gtcgatgaag
    28021 agggtcacct cggcgctgtt gcgcggcggc cgatcctccg agcccgcgaa gatcacgtcc
    28081 tccatggccg cgccgcgcat gctcttgtac gaggtctcgc ccatggccca gcgcagcgcc
    28141 tcgagcaggt tcgacttgcc gcagccattg gggccgacca cgccggtgag cccggtctcg
    28201 atcacgagct cggtcggctc gacgaaggat ttgaagccga gaaggcgaag ccgggtaatc
    28261 ttcacgtgcc aggagcctca atgcaagcgg tcgcgcccgc gacgacacga atgaactccg
    28321 ccgcaacctc aagaataccg agaacgggta aacggaattg ccgcgtcacg ccgggcgaac
    28381 aactgcggtc atgaggatga cggcgcgggc gcctgctcga tcatggcctt gatctcttcg
    28441 aaggtgacct ctccttgcgc cttgcggcca ttgatgaaga aggtcggcgt cccgatcacg
    28501 ccaaattgcc gcccccgctg cttcacctcg ttcaagccgt cgataatggt ttgattcgat
    28561 aggcatttgt caaacgtctc gcgcgtcatg ccgctcgact tggcgacggc gaagatcgcg
    28621 tcggggcgga cgtcttggct gacccattcc ggctgccggg tcaggaactg gccgagaagg
    28681 aagaaatact tgtcctcggg cgcgcagcga ttggcgatgg ccgcgttccc cgaggtgcgc
    28741 ccgatcggga actcccgcac gatgaagcgg accttgccgg tgtcgatgaa ctccttcttc
    28801 accctcggaa acagcgtctt gtgaaagttg gcgcagtgcg ggcaggtgag cgagacatac
    28861 tcgatcacgg tgatcggcgc gttcggattg ccgagcgcgc gctccccgag gggtcccgtc
    28921 gccatcagct cggcttgagg cggaacgtag gtcgccttgt cgatcggcgg cgtgggacct
    28981 ccggcgccgc cgccgcagcc ggcaagtgcc agagccatag caagggcaat cgacggtttg
    29041 gccgcgctgg cgcgcatcag gcgaacacct attgacccga aggcgcctca ggttcgctcg
    29101 gggcctgtcc ttcggcgctc gctgggcctg cgggcggggt cgcgcctccc gacagcatgg
    29161 cctcgaaacc cgcgaccgtc tgctcgccgg tgagccgttt gccgttgatg aagaaggtcg
    29221 gcgtggaatc gacgccgaaa gtttcgtggc cgcgttcccg gatctcgacg atcttttcga
    29281 acagggcttt gtcgccgatg cactggtcaa atttttcctt gctgaagccc gcctgcttgg
    29341 cgatctgcaa cagcttgtca gtgccctctg tgccggcgac cgcccaggtc tcttgcgtgt
    29401 cgaacaaggc gccgaccatc gggtaatagc ggtcgggtcc ggcgcagcgc gcgagcatcg
    29461 aggcttgagc ggcgagctgg tccagaggga attcgcgcaa gatcagccgc gcttggccgg
    29521 tatcgatata tttggtcttc agctccggca gcaccgtctt gtggaactgg gcgcaatggg
    29581 ggcaggtcat cgaggcatat tcgacgatcg tgtcgggcgc atcctccttg ccgagagaga
    29641 gatcgggcaa gggaccgggg gccatcagct ccgcgaggcc gggatccgtt ttctcgcctc
    29701 ccgctccccc ggcggaactg tcgcgccaat agaagatgcc gatggcaagc gccaccgccg
    29761 caacggcgcc ggcgacataa agccaaatgc tgttcgcggc cgccgcgtct tggccgcccg
    29821 gcgtttctcc tcgcggagac ttgccggccg cctttttctc cttctttgcc actctttctg
    29881 ctcccgatga tggatttccg gccctgaagg ccgtgccgcg cctacaatcg ttgaaacggg
    29941 tccctcatgg caagagcgag aaatcgcccg atcgcctcag cgtctgtgcg gacccgcgga
    30001 cgcaagccgc agcaaggctt cgcggaggcc ggcgtcctcg atggccgccg aagggggaag
    30061 cgcgtcgggg tcgatcggtt gcggggcggc gggggcggcc ggcgcgggct ttcgcgtcac
    30121 cggcgcctgc aagatgcgca tctcggccac ggcgcgatag ccgaaatggc tgttgacccg
    30181 ctccaggatt tggcaggacc ggtgctgcac ctcgattgcg cgcggaccat cgacgcgaag
    30241 aataaggatc gcgccttcgt ctcttcgtcc ctgatgccgc accgtttcgc ctgtgtcatc
    30301 ccggtgccgc ggccagacca ctctgtcggg catggtgaat tgcgcaagct cggcccccac
    30361 gatggacggc cattcggcaa gcagcgccgc cgtggcaaag ccgcgggcgc gtgccgcggg
    30421 gtcgagcgcg cgggcggtat acgcgccgat gggtcgcgcc gtggcgtgcg ctctggccag
    30481 ggctcttcgc tcgctgtcgt ccatgatgct tgaaacggtg gagggatcgc gcccgccgct
    30541 tgcttcgatt ccatcgagct tacaccatgg ctcggagaga agcgcggccg gagttcggtt
    30601 catgcagaag agaaaggcgg gcgagaggcc gaagaagcag gacatcgccg ctgcgctcct
    30661 tgcctggtat gcccgcgaga ggcgggaatt gccgtggcgc gccgcgccgg gcgagacggc
    30721 ggacccttat cgcgtctggc tcagcgagat catgctgcag cagacgacgg tgaaagccgt
    30781 cattccccgt tacgcagcct tcttgcgcca atggcccgat gtgaaggcgc tcgcaacagc
    30841 cgagcttgga caggttctcg ctgcctgggc cgggctcggc tattacgcgc gggcgcgcaa
    30901 tctgcacgcc tgcgcacgcg ccgtggtgga gcagcatggc ggcggatttc cggagagcgc
    30961 ggccgagctg cgcaagcttc cggggattgg cgactatacc gccgcggcca ttgcggcaat
    31021 cgccttcggg aagcgcgcga ccgccgtgga cggcaatatc gagcgcgtcg tcgcccggct
    31081 cttcgccgtc acgacaccgc tgccagaggc gaaggccgag atcagggcgc tcgccgagac
    31141 ccttacgccg gcggagcgcg cgggcgactt cgctcaagcg atgatggatc tcggcgccac
    31201 gatctgcacg ccgcggcgcc cggcctgtgc cctctgcccg ttgcggccgg agtgccgcgg
    31261 ttttgccgat ggcgtggcgg aagcgctgcc ctaccgcgag gagaagcgcg aacgtcccac
    31321 gcgccgggcc gcggccttcg ttgcgctcag accggatggc gccgtcctgc tccgcaagcg
    31381 gccacttaaa ggcttgctcg gcggcatgct cgagacgccg tcctcgccct gggaagaggc
    31441 gcggccgaac ggaaaattgc tgggtcaata cgcgccgctc gaggccgatt ggcgcaagct
    31501 gccgggactg gtgcagcaca gcttcaccca tttccatctc gagctcaccg tctatcgcgc
    31561 cgatgtcggc cggagcgcca agccgaagcg cgcggcggtg ccggaacttt gcggctttgt
    31621 cgcacggcgc gacctcgcgc gcgcggcgct cccttctgtg atgcggaaag tgttaaatca
    31681 cgccttccag tcgaaggcgc gaacgctcaa ggctggacgg caagcttcgc ccgcgctgcc
    31741 tcgcgcaagg cgtcgatcgg cttgagcgtt ccttccgcct cgtaatgcca aaaggtccag
    31801 ccgttgcagg cgatcgcccc ttgcacttgt gcgccgatct tgtggatcga accttgcgcg
    31861 ccggcgcacg cgatcgagcc atcggcccgc actttcgcct catgccgcgc cgccgggtcg
    31921 taaagcaccg tgccaggcgc gatcaaccca agctcgacga gcgcgccgaa cgggatgcgt
    31981 tgctccgcgc gcttgccctt ggcgacttga agcgcggccg aatcgaaccc ttcgagagac
    32041 ttgagccggg cgcgcgccgc ctcggcatag gccgcgtcgc gctcgatgcc gatgaaggcg
    32101 cgtccgagcc gcttggcaac gaccgcggtc gtgcccgaac cgagaaacgg atcgagcacg
    32161 acatcgccgg ggttactcga agcaaggatg atgcggtgga gcagggcctc gggcttctgg
    32221 gtggggtggg tcttgcgccc acaatcctcg cgcagccgct ctgcgcccga gcagatcgga
    32281 aagagccagt ccgagcgcat ctgcaaatcg tcgttcagcg ccttcatcgc ttcgtaattg
    32341 aaggtgtagc gcgccttcgg tcccatcgac gcccagatca gcgtctcatg cgcgttggtg
    32401 aagcgccgcc cgcgaaaatt cggcatggga ttggtcttgc gccacaccac gtcgttcagc
    32461 acccagaagc cgagatcctg aagggtcgcg ccgacgcgga agatgttgtg atagctgccg
    32521 atcacccaca gagagcccga gggcttgagc acgcggcggc aggcaatgag ccagtcgcgg
    32581 gtaaaggcgt cgtaggcgga aaaacttccg aacttgtccc aatcgtcgtc gacggcgtcg
    32641 acgcggctgt tgtccggacg gtagagctcg ctgttgagct gaaggttgta gggcgggtcg
    32701 gcgaagacga gatcgaccga ggcttcgggg aggcgcgcga gcgcgtcgat gcaatcgccg
    32761 acaatgacgg tgtcgagcgg gagctgggct cccggcttgt cccccagacg catgactcta
    32821 ctcgggttac gcaacacttg aatacgcgcc ccgtgtgatg cgggacgggc atcagcttga
    32881 aggatttaga gtaaagacgc aattaacccc ggcattggct tggcgccaag gccatgtcac
    32941 aagggtcgaa ccatgctcgg actcttgcgc cggaaattcc tcaaaggagc cctcggtctg
    33001 gcgctcctga agctggccgg cttgcgggcc tccgcggcga aaatgagggg gaaggggctg
    33061 acgtcatgac cagggacatc gtgatgctgc acggggccaa tgaagggtcc tggtgcttcg
    33121 acaaattcaa ggcggtgttc gagagcgtgg gctggacctg ccacgcgccg gacctcatcg
    33181 ggcatgggac gagggcgacc aattccgata agaccctggt cggcataggc atcgcggact
    33241 accaagccga gctcgaggcc ttcctcaaga cgatccctcc gcggccggtg cttctcggtc
    33301 attccatggg cgcggtcctc gcccagcttc tcgccgccaa gggccttgcc cgcgcgcttg
    33361 tgctggtggc gccgggggcg cgcgccggga tcctgccgca gaccgagggg gagaagaagt
    33421 tcggccaaga cctgatgggg ctgggcgcct tctggaagac tgtcatcaat cccgacttcg
    33481 gtctcgcgcg cgtcttgact ttgaaccgtg tccccgaggc cgagcagcgc gcggtgttcg
    33541 accggtttgg cccggaatcg ggccgtgcct ttttcgaact gttcttctgg atgttcgatt
    33601 cgacgagagc cacggtcgtc gatatcagct ccgtcacctg tccggtgctt tgcctcgcgg
    33661 gcgccgacga caatctcgtg tcggttgcca cagtgcgggc gacggcagcg ggctatcccg
    33721 gcgcgactct gtgggagctt gaaggccacg gccatatgct cgtgctggag cccggggccg
    33781 aagcgatcgc gcggcgcatc gcagactgga ttccgagcta gatctcgagg ctcccgagaa
    33841 cgggcagctc atccgcgcct gtctcgagca ggcgcagcgc gcgctcgatc gggctgaacg
    33901 agcggcggtg atgggtgcag acaccgtgct tctcgatggc gcgagcgtgt tcctgcgtgc
    33961 cgtagccctt gttgctctcc cagccatagc gccggtagcg gcgggcaagc ttcaccatca
    34021 gccggtcgcg cgtgaccttc gccacgatgg aggccgccgc aacggacagc gaaagcccgt
    34081 cgccgccgac aagggcgcgc gcggggcagg gaaagcggcg cggaatgatg ttgccgtcga
    34141 tcagggccga gcccgccggc acgccgagac cgcgataggc ggctctcatg ccccacaggc
    34201 tcgcctggag aatgttgtcg cggtcgatgc gccggacgtg gccgatgacg atcgacacga
    34261 tcgaagtggc gataatctcg gcatagcgat cctcgcgcgc ctgcggcgtg agaagcttcg
    34321 agtcgttgag gccttcgggg atccggtcgg gattgaggat cacggcggcg accacgacag
    34381 gtcccgccca cggtccgcgt cccgcctcgt cgatgccggc cacggcgccg cgatacttgc
    34441 ccatcgcctc ggcctcgaag tcgaaggagg gcagagggcg aaccggcagc gactcggcgg
    34501 caagtcccat ggcccgggac tctggcgcaa accccgcacg cttggcaact cgttcggcgg
    34561 gaacgcgcgg gcttctgtgg aaaagcggct agagcagggt gagctgctgg ccgggacgcg
    34621 aaggaggcgt gaacaggtcg gtgcggagtg cgaccgaatc ctcattgagc ccgagccgct
    34681 gcgcagcgag ttcgaatctc cgtccgatca tccaggcgta aggtccgctg ccaacctgac
    34741 gctcgcccca tttcgcgtca taaagcttgc cgccgcgcgt ggaccgcatc agcgacagca
    34801 cgcgcttggc gcgatcgggg cagtttgctt gcagccactc ggtgaagaga tcgccgatct
    34861 cgagcggaag cctgagcagc acgtagcctg cctgcttcac gcccaccgcc gccgcgcgtt
    34921 cgagaatgcg ctccatctcc gcgtcggtga gcccgggaat gacgggcgcc accatgaccg
    34981 aagcaggcac gcccgcgtcg acaaggcgct gaagcgtctc gagccgcttg tcgggcgtgc
    35041 tcgctcgggg ctccatggag cgggcgagct tgcggtcgag cgtggtcacc gacagagcca
    35101 ctttcaccag gccgcgcttg gccatcgagg tgagcaggtc gagatcgcgc aaaacgagag
    35161 ccgactttgt gacaatgccc acgggatggt tcgccgcgct caacacttcg aggatgcggc
    35221 gcatgatgcg atagcgccgc tcgatcggct gataggggtc ggtgttggtg ccgatggcga
    35281 tggtccgcgg ctcatagcca ggctcggcaa gctcccgctc gagcgcctgc gcggcattgg
    35341 tcttggcaaa cagcctggtc tcgaaatcga ggcccggcga cagcccgagg aaggcgtgcg
    35401 tcggtcgcgc gaagcagtag atgcagccat gctcgcaacc ccggtaaggg ttgatcgaac
    35461 ggtcgaagcc gatatcggga gattcgtttc gcgtgatgat gcgccgcgcc gggacctcct
    35521 gcacctcggt ggtgagcgct tcgagctcgg cgagcgatcc ccagccgtcg tcggccgcct
    35581 cgtagccgat cggctcgtag cggcccgagc ggttggtctg cgcccctctc ccatgccggc
    35641 ggcccgcggc aacgccgccc gcctcggggg gaaggccctg cgaatcgggc gcttttctga
    35701 gcgaaatcga catatcgcct atataggcgc tgattgtgaa caaagcaaga acgttgggct
    35761 agtgagcacg gcgcaggcga ttgccgggcg aggggcggcc gctataggtt tcgcgccatg
    35821 atctcggtcg tcatcccgac gctgaacgcc gagcggagcc tcgcccacag ccttgccgct
    35881 ctcgttccgg cggtggtcga gggcgtggtg cgggaggcga tcgtcgtcga tggcggctcg
    35941 accgacggaa cccgcgtcgt cgccgaagct gccggcacgc atctgatcga ggccgggcgc
    36001 gggcgcggaa cccagctcga agcgggcgca agcgcggcgc aagccgattg gcttcttttt
    36061 cttcacgccg acaccgtgct cgatcccggc tgggccgagg aggcgcaagg cttcatcgag
    36121 cgcgtggaaa gcggaaggcg cggccaggcc gcggcctcgt tccggttcgc gctcgacgat
    36181 gacggcatca tgccgcgttt cgtcgaattt ctggtggggc tgcggtgccg gcttctcggc
    36241 cttccctatg gcgaccaagg gcttctcatc tcgcgccgcc tctaccggcg gctaggcggt
    36301 tttcgcccgc tgccgctgat ggaagatgtc gacctcgtgc gcaggctgaa gcggcgcgag
    36361 ctggtgatgc tgaaatcgcg cgcagtgacg agcggcgagc gctaccggcg ggaaggctat
    36421 ctggcgcgga gcctccgcaa tctcagctgc atcctgctct attaccttca cgtgccgcca
    36481 cgggtgcttg cgcggctcta tggctaggcg gagcggcggt cgcaagcgcg gcgcgagctt
    36541 cgccaagcgg ctcgtcatca tggccaagag cccggatatg ggccgggtca agcggcgcct
    36601 ggcgggcgag atcggcgaga gcgccgcgat cagattctat cggggctgcc tctctcacac
    36661 cctgttgcgc ctggcgcgcg acccgcgctg gctgaccgtg cttgcggtcg atcaggggcg
    36721 cggtcgcgcg aggcgctttt cgccattgcg gaagcggctc acgttcctgc cgcagggcga
    36781 cggcgatctc ggccgtcgca tgcagcgcct gttcacgcgc ttgccgcccg gcccggtggt
    36841 catcgtcgga acggacattc cgtccgtccg cccgggccat atcgccgagg ccttcaggct
    36901 gctcggcggc gccgatgcgg tcttcgggcc ggcgcccgat ggcggctatt ggctggtggg
    36961 actaaggcgg acaccgcgcg tgccggcgcc ctttgccgcc gtccgctggt cggggccgca
    37021 cgcgcttgcc gacacgctcg ccaatctcaa gggcaagcgt gtggccatgg cggcgcgtct
    37081 gagcgatgtc gacacggcgg aagatttgcg gcgtgagagc gcgcgttccg ggcgcctgat
    37141 ctcgacgatc accacgctct agttagaacc gccaagcgaa gcggccctga accgcgttgt
    37201 cctcgacgtt gtcgccgaac tgccccgaat aggagacgcc ggccgtcgta taaggcccaa
    37261 acccaaggtc gagaccggct tcgatcagcg cggtgtcttc cgccagcggc acgccggtga
    37321 cggtaaagcc gatgccggtc gaggcgaagg ccagagccgc tccggtcctc acatcgtcga
    37381 aggcgtgctg ccaggcggcc gacacatgcg gtgttaccgt gacgctgttc caagccatgg
    37441 tggtggcggc gcgcaagccc agcgtcgaat agccgacatc cagatcgtcg cctgacccgc
    37501 gaagcccggc aagcgcgccg ttctccctga acgtgtcagg atcgatggag acgtaggcga
    37561 gaccggcgaa aggctcgagc gcggtgcggc ccctgacgat gggataggcc acctcgccga
    37621 agatctgccc ggtatcggcg tcgtaggagg ttttctcgcg ctcgaagaag ccggggaaga
    37681 tcactgcgcg ggaggtatcg acctcgttcc acgaccaggc gccgccgcct ctgagcgcga
    37741 ccggcccggc catgccgcca ccatagccgg cgagatggaa gctctccacg tcggcgccgc
    37801 tatggcgcgc ggcgaccgag acgtcagagt tggaatagcc ggtggcaaag ccgagccgcc
    37861 agctgccgct cacgcgcgca tcgagccccg agacgatccc gccgagatcg cgcgaggccg
    37921 aagccgcatt cagattgccg tcgaaatcgc cccaggcgcc atagccccgc gcccagaagg
    37981 caagtcccgg accataggcg ggcggagcca aggacttgtc gtcatagcca agcgccatgg
    38041 ccttcgcatc gagcgaagcg acttgcgggc cggcggtgcc aagcgaagcg acctggctcg
    38101 catcgt
//
LOCUS       DASUQP010000232        12747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2022808, whole genome shotgun sequence.
ACCESSION   DASUQP010000232 DASUQP010000000
VERSION     DASUQP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022808"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFY74_10765"
     CDS             complement(<1..513)
                     /locus_tag="VFY74_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5932211.1"
                     /translation="MAAETPVCDFGWEAPSFRLPGVDGKTYSLEELKGPNGTLVMFIC
                     NHCPYVKAVIDRIARDVNELRGLGVSAVAISSNDAVHYPEDSFANMKRFAAAHGFTFP
                     YLYDESQDVARAYDAACTPDFFGFNSKLELQYRGRLDESRKEAAPAGVRRDLFEAMKQ
                     VALTGQGPREQ"
     gene            592..2601
                     /locus_tag="VFY74_10770"
     CDS             592..2601
                     /locus_tag="VFY74_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HEX5932212.1"
                     /translation="MAKPDGFCNRAEACEAHDNVPLSPTQGDTIGDLILRRYSRRAVL
                     RGTLGATAAAALFGPALLAGRAARAVDEADRFAFAEVAAGVDETHHVAHGYRANVLLR
                     WGDPLFPDSPAFDPLRQSAEAQLRQFGYNNDYIAFFPLDDSGKRGLLCVNHEYTNEEV
                     MFPGLVRQDVICFPDMTAELVAIEMAAHGVTIVEIARGEDGWRPVLDSDYNRRISPTN
                     TPMTVDGPASGHARMKTSADASGRNILGTLNNCAGGVTPWGTYLTAEENFHGYFWSAL
                     CAEGGKAPAGLGGAQAKSYERYGVPGLWQAWGKFVDRFNVDKEPNEPNRFGWIVEIDP
                     FDPASVPVKHTALGRFRHEGAECLVNKDGRVVIYCGDDARFDYVYRFVSDGTYDPDDR
                     AANRRLLSQGTLSVARFDADGSLNWLPLVFGHGPLTPENGFADQGDVVIDARLAADLL
                     GATRMDRPEDVQPNPATGRVYVLLTNNKDREAGQEDAANPRAENVFGHIIEMTPPDGD
                     HAADRFAWDLLVRCGNPSVAAVGALWNPATSDNGWFACPDNAAVDADGRLWISTDQGE
                     AWPTGRADGLYALETEGARRRMAKLFFRCPVGAELCGPCFTPDQETVFVAVQHPGTDG
                     TDRYKGFERASTFADPATRWPDFHPDMPPRPSLLAITKIGGGKIA"
     gene            2693..4759
                     /gene="parE"
                     /locus_tag="VFY74_10775"
     CDS             2693..4759
                     /gene="parE"
                     /locus_tag="VFY74_10775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX5932213.1"
                     /translation="MSKRPVRKAAASRDLFAKLEDAPRKAPSKPAKPQARGASSSRAE
                     AGYTAADIEVLEGLEPVRRRPGMYIGGTDEKAMHHLFAEVLDNAMDEAVAGHADHIGV
                     AFEEDGFLTVTDNGRGIPVDPHPKFRKKSALEIIMTTLHAGGKFNSKVYETSGGLHGV
                     GVSVANALSETMDVEVARGQKLYRQSYTRGAPDGPLKNLGAIKNRRGTRVRFRPDPKI
                     FGKGAAFRPARLFRMARSKAYLFRGVEIRWSCAKAMLAGDSETPESAVFHFPGGLKDF
                     LGSRIEGQEAVTKDFFADRIEKTEGSGSVEWAIAWIASDDGFLSSYCNTVPTPDGGTH
                     EAGLRAALTKSLRGYGELTGHKRVGQVTADDVLGTAAAMLSVFIREPEFQGQTKDRLS
                     SPEAARIVEGAVRDAFDHWLTGNPSQANLLLDWVVDRSEERLRRKKEKEIGRQTATRR
                     LRLPGKLADCAVSGAQGTEIFIVEGDSAGGSAKQARDRATQAVLPLRGKILNVASASS
                     TKLADNQLLSDLIQALGCGTGARYREEDLRYEKVVVMTDADVDGAHIASLLITFFYRE
                     MRGLIEGGHLYLAVPPLYRLSQGAKTFYARNDAHKDELMRKEFNGKGKVDVSRFKGLG
                     EMMPKQLKETTMDPKARILLRVEIADDDKATFKLVDQLMGNKPEARFRFIQERAEFAK
                     PDALDI"
     gene            complement(4761..5234)
                     /locus_tag="VFY74_10780"
     CDS             complement(4761..5234)
                     /locus_tag="VFY74_10780"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5932214.1"
                     /translation="MNANDHAEVAIMPPILFLGALAAGALLSFLIPIGPGLGSANALA
                     LTVGLTFVLIGFALVARSVHNFRLARTSVVPSEPATALVATGPYRFTRNPIYIGFVLA
                     YFGFAIVLTSMWVLLLLIPVLVILQRGVVLREEAYLERKFGDVYCKYKARVPRWL"
     gene            5517..7007
                     /locus_tag="VFY74_10785"
     CDS             5517..7007
                     /locus_tag="VFY74_10785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5932215.1"
                     /translation="MSFSNLGLSPKVLSAVEAAGFVTPTPIQEKAVPAAIAGRDVLGI
                     AQTGTGKTASFVLPMLTRLERGRARARMPRSLILEPTRELAAQVAEHFDILGKSHKLT
                     VALLIGGVSYEDQDRKLDRGADVVIATPGRLLDHFVRGKLLLNGIEILVVDEADRMLD
                     MGFIPDVERICKLLPFTRQTLFFSATMPTEIQRLADKFLHNPERIEVAPQATAAATIV
                     QRLKKAPDEAAGKREALRALMREIDVKNAIIFCNRKKDVGILHRSLQRHGFNVGALHG
                     DLDQHQRTATLDAFRSGQIAFLAASDVAARGLDIPEVSHIFNYDVPIQPEDYVHRIGR
                     TGRAGREGFAATLVTPRDYKALKAIEKMLGYEIAWIDGAPSEEEQKEALDSRPYGNGR
                     HRRGGRSRRDKHPSGPHAKTGHPSRPHGPNVQRSAPQGSHYGKKQDERRHDRPKHPHP
                     GERKSAHGHATPAPQQKKSQPEPSSSRQQGMGDHVPAFMMRPVRVT"
     gene            complement(7750..8130)
                     /locus_tag="VFY74_10790"
     CDS             complement(7750..8130)
                     /locus_tag="VFY74_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX5932216.1"
                     /translation="MAVRPRPQVMRLTEAAAARIRALQAASSKTVEGVRIGVKNGGCA
                     GMEYTMEYAQAHGPHDEIVEDKGVKLLIDPSAVMFLLGTEMDYRTDRMRSGFVFNNPN
                     QTSACGCGESVALKPAEDSAQDSS"
     gene            complement(8175..8636)
                     /locus_tag="VFY74_10795"
     CDS             complement(8175..8636)
                     /locus_tag="VFY74_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEX5932217.1"
                     /translation="MIERTQKDDAPAREAEPQSEPEAAGGESGEIKAGVGATGGGEGA
                     GSALPPDEVERLKGDLIAALKTVYDPEIPVDIYELGLIYRLDVDDDRNIEIDMTLTAP
                     GCPVAGEMPGWVENAVSAVPGVGQVKVNLVFDPPWNTSRMSDEARLDLNMF"
     gene            complement(8650..9915)
                     /locus_tag="VFY74_10800"
     CDS             complement(8650..9915)
                     /locus_tag="VFY74_10800"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5932218.1"
                     /translation="MTVLTPTKQDRRPAAAPFDVEAIRADFPILSREVNGKKLVYLDN
                     GASAQKPRVVLDTTLRAYADEYANVHRGLHYLSNLSTAHFEQARESVRRFLNARSDEE
                     IVFTRNATEAINLIASSYGAPDIGEGDEILLTIMEHHSNVVPWHFLRERQGAVLKWAP
                     VSDDGEFLLDAFERLITPRTKMIAITHMSNVLGTIVPLKEVVRIAHKRGIRVLADGAQ
                     GAVHLPVDVQDLDIDFYAFTGHKLYGPTGIGVLYGKKEHLEHMRPYQGGGEMIGTVTT
                     DMITYAKPPHRFEAGTPAIVQAIGLGAAIDYVESVGRERIAAHEAALLDYAMERLGEL
                     NWLRIFGRAKDKGSIIAFNMEGAHPHDVSTILDRYGVAVRAGTHCAEPLLARYGVTST
                     CRASFAMYNTMEEVDTLVEGLHKAREMFA"
     gene            complement(9927..11279)
                     /gene="sufD"
                     /locus_tag="VFY74_10805"
     CDS             complement(9927..11279)
                     /gene="sufD"
                     /locus_tag="VFY74_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5932219.1"
                     /translation="MDLPVQQFRTKAEQAYLDLFEAAENALPGARDPFVSDLRTTAIA
                     AYGGQGLPHRRIEAWKYTDLRARLTDVNPLGKSDGAAVNEGALSQALGAEIARLPAYR
                     VVLVEGDLSADLSDLAGLKSAGVEAISLAVALEKPPAWLKKALGHVNPHDDDAVLALN
                     TALMTGGLALRIGEGVILDKPIHVIALDGAGDPASTFTRNVVIAEPGSSATILESFGS
                     LGLRGSQRNAATELRIGAKAAVKHIKLQRDGDDAVHLCTWLVELGRDARYDAFQFSTG
                     AALARNQIYLRFLGEGAAADISGAFLLRDRQHCDTTLLVEHRVPHCTSREMFKGVLDG
                     EARGVFQGKIIVSPGAQKTDGKQMAGALLLSETAEFDSKPELEIFADDVVCGHGSTSG
                     QIDDELLFYLEARGIPEAEARALLVQAFVGEAIERVEDEALRDALINASAAWLGISAD
                     "
     gene            complement(11291..12040)
                     /gene="sufC"
                     /locus_tag="VFY74_10810"
     CDS             complement(11291..12040)
                     /gene="sufC"
                     /locus_tag="VFY74_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX5932220.1"
                     /translation="MLEIKNLHVNVDGREILNGLDLTLPQGEVHAIMGPNGSGKSTLA
                     YVLAGKAEYEVTAGSVTWKGQDLLAMEPSERAAAGVFLAFQYPMEIPGVATMTFLRTA
                     VNAVRKARGEAELSTPDFLRFVREKAKALKIEPEMLKRPLNVGFSGGEKKRNEILQMA
                     LLEPALCVLDETDSGLDIDAVRIVAEGVNALRSPERAMLVITHYQRLLNYIVPDRVHV
                     LSEGRVARSGGPELALELEESGYAEFGEAAA"
     gene            complement(12040..>12747)
                     /locus_tag="VFY74_10815"
     CDS             complement(12040..>12747)
                     /locus_tag="VFY74_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932221.1"
                     /translation="DSTKTDEEAILLVSDDIAEVKALLRQASQQLLRIERQLKAALPQ
                     RIPVPKKPRQRIDEQGAGEVINRLKERLLRGEQIEGQLRVFTVKPELQRIARLLGMTN
                     TKLPPKDELIRRISTRLRQGVSVTSGISETVTQNHDSGNRSANRDKGAEQIMKSVCER
                     LDKLGIAYSILRSGELFFRRDDGVPMYVRAYPNNQYDWTFKGSHNDTNHLDYRAPHKR
                     SMGELIAAIEQFRKASE"
BASE COUNT         2306 a         4149 c         4091 g         2201 t
ORIGIN      
        1 ctgctcgcgc ggcccctgcc cggtcagcgc aacctgcttc atggcttcga agagatcgcg
       61 gcggacgcct gcgggcgccg cttctttccg gctttcgtcg agccggcccc gatattgcag
      121 ctcgagcttt gaattgaagc cgaagaaatc gggcgtgcag gcggcgtcgt aggctcgcgc
      181 cacgtcctgg ctctcgtcgt agagataggg aaaggtgaag ccatgcgccg cggcgaagcg
      241 cttcatgttg gcgaaggaat cctcggggta atgcaccgcg tcgttggaag agatggcgac
      301 ggcactgacg ccgagcccgc gcagctcgtt cacgtcgcgc gcgatgcggt cgatcaccgc
      361 cttcacgtaa ggacagtgat tgcagatgaa catgaccagc gtgccattcg gccccttcag
      421 ctcttccagg ctgtaggtct tgccgtcgac gccgggaagg cggaacgagg gcgcctccca
      481 gccgaaatcg caaaccgggg tctctgccgc catcgtgccg ttccgtttcg caaatctgtt
      541 acggtgcggg cctactctcc cgcgccgcaa acgtaatcca ggacttggca tatggcgaaa
      601 cccgacggct tctgcaacag ggccgaggcc tgtgaggcgc atgacaatgt gccgctcagc
      661 ccaacgcagg gcgacacgat cggcgacctc atcctgcgcc gctacagccg ccgcgcggtg
      721 ctgcgcggca cgctcggggc gacagccgcc gcggcgctgt tcggtcctgc gctgctcgcg
      781 gggcgcgcgg cacgcgccgt ggacgaggct gaccgcttcg ccttcgcgga agttgccgcc
      841 ggggtcgacg aaactcacca tgtcgcccac gggtaccggg cgaacgtgct gctccgctgg
      901 ggcgatccgc tctttcccga ctcgcccgcc ttcgatccgt tgcggcagag tgccgaggcg
      961 caactgagac agttcggtta caacaacgac tacatcgcct tcttcccgct cgacgattcg
     1021 ggcaagcgcg gcctcctctg cgtcaatcac gaatatacca acgaggaggt catgttcccg
     1081 ggcctcgtcc gccaggacgt gatctgtttc cccgacatga ccgcggagct cgtcgcgatc
     1141 gagatggcgg cccatggcgt caccatcgtc gagatcgcgc gcggcgagga cggttggcgc
     1201 cccgttctcg acagcgacta caatcgccgc atcagcccca caaacacgcc gatgacggtc
     1261 gatggaccgg cttcagggca cgcgcggatg aagacgagcg ccgacgcctc tggccgcaac
     1321 attctcggca cgctcaacaa ctgcgccggc ggcgtcacac cgtggggcac gtacctcacg
     1381 gcggaggaaa atttccacgg ctatttctgg agcgcgctct gcgccgaggg cggcaaggcg
     1441 ccggcgggcc tcggcggcgc gcaagcgaag agctatgagc gctatggcgt gcccggcctg
     1501 tggcaagcct ggggcaagtt cgtcgacaga ttcaatgtcg acaaggagcc gaacgagccg
     1561 aaccgcttcg gctggatcgt cgagatcgat ccgttcgatc cggcttcagt gccggtgaag
     1621 cacaccgcgc tcggccgctt tcgccacgaa ggcgccgagt gcctcgtcaa caaagatggc
     1681 cgcgtcgtca tttattgcgg cgacgacgcc cgtttcgact atgtgtatcg cttcgtctcc
     1741 gacgggacct acgaccccga tgaccgcgcc gcgaacaggc ggctcctttc gcaaggaacg
     1801 ctgtctgtcg cgcgatttga cgccgatggc agcctcaact ggcttccgct cgtgttcgga
     1861 cacgggcccc tcacgcctga gaacggattc gccgatcagg gtgacgtggt catcgatgcg
     1921 cgcctcgccg ccgacctgct tggcgccacg cgcatggacc ggcccgagga cgtgcagccc
     1981 aatccggcca ccggccgcgt ctatgtgctc ctgaccaaca acaaggaccg cgaggccggg
     2041 caggaggacg ccgccaatcc gcgggccgag aacgtgttcg gtcatatcat cgagatgacc
     2101 ccacccgacg gcgatcatgc ggccgatcgg ttcgcctggg atctgttggt tcgctgcggc
     2161 aacccttcgg tcgcggcggt cggggcgcta tggaacccgg cgacgtcgga caatggctgg
     2221 ttcgcctgcc ccgacaacgc cgcggtggat gccgacgggc ggctatggat ttcaaccgac
     2281 cagggcgagg cctggcctac ggggcgcgcc gacggcctct atgcgttgga aaccgaaggc
     2341 gcgcggcgtc gcatggcaaa gctcttcttc cgttgcccgg tcggcgccga gctctgcgga
     2401 ccctgcttca cgccggacca ggaaaccgtg ttcgtcgccg tgcagcaccc ggggaccgac
     2461 ggcaccgatc gctacaaggg tttcgagcgc gcatcgactt tcgccgaccc tgcgacgcgc
     2521 tggcccgatt tccatccgga catgccgccg aggccgtcgc ttctcgccat caccaagatc
     2581 ggcggcggca agatcgcgtg aggttttcgc tgtttttctc gctcttgaaa aagaagcggg
     2641 cgcgtgcgag ctactcttgt tccctgtacc cgtgagattc gtggagcccc tggtgtccaa
     2701 aagacccgtg cgcaaagccg ccgcatcgcg cgacctgttc gccaagctcg aagacgcacc
     2761 gaggaaagcg ccaagcaagc cggccaagcc gcaggcgcgc ggcgcgtcct cctcgcgcgc
     2821 cgaggcgggc tacacggccg ccgatatcga ggtgctggaa gggctcgagc cggtgcgccg
     2881 gcggcccggc atgtatatcg gcggcaccga tgagaaggcg atgcaccatc tcttcgccga
     2941 ggtgctcgac aacgccatgg acgaggcggt cgccggtcat gccgaccata tcggggtcgc
     3001 gttcgaggag gacggcttcc tgaccgtcac cgacaacggc cgcggcatcc ccgtcgatcc
     3061 tcacccgaaa ttcagaaaga agtccgcgct cgaaatcatc atgacgacgc tgcacgcggg
     3121 cgggaagttc aactccaagg tctacgagac gagcggcggc cttcacggtg tcggcgtctc
     3181 ggtcgcgaac gcgctgtcgg aaacgatgga cgtcgaggtc gcgcggggcc agaagctcta
     3241 tcgccagagc tacacgcggg gggcgcccga cggcccgttg aagaacctcg gtgcgatcaa
     3301 gaaccgccgc ggcacgcgcg tccgcttcag gccggacccc aagatcttcg gcaagggagc
     3361 cgccttccgt ccggcgcggc tgttccgcat ggcgcgctca aaggcctatc tcttccgcgg
     3421 cgtcgagatc aggtggtcgt gcgccaaggc gatgctcgcg ggcgacagcg agacgccgga
     3481 gagcgccgtt tttcacttcc ccggcgggct gaaggatttc ctgggcagcc gcatcgaagg
     3541 acaggaggcc gtgaccaaag acttcttcgc ggaccggatc gagaagaccg aaggttcggg
     3601 ctcggtcgaa tgggcgatcg cctggatcgc gagcgacgac ggcttcctca gctcctattg
     3661 caacaccgtg ccgacgcccg acgggggcac gcatgaagcg ggactgcgcg ccgccctcac
     3721 aaagagcctg cgcggctatg gcgaactgac tgggcacaag cgcgtcgggc aggtgacggc
     3781 cgacgacgtg ctcggaaccg ccgccgccat gctgtctgtg ttcatccgcg agcccgagtt
     3841 ccaggggcag accaaggaca ggctgtcgag ccccgaggcc gcccgcatcg tcgagggcgc
     3901 cgtgcgcgac gccttcgacc actggctgac cggaaacccg tcgcaggcga accttctgct
     3961 cgattgggtg gtcgaccgct ccgaggagcg cctccgccgc aagaaggaaa aggagatcgg
     4021 ccggcagacg gcgacgcggc gcctgcggct ccccggcaag ctcgccgatt gcgcggtgag
     4081 cggcgcgcaa ggcaccgaga tcttcatcgt cgagggcgac tcggccggcg gctcggccaa
     4141 gcaggcgcgc gaccgcgcga cccaggccgt actgccgctc agaggcaaga tcctcaacgt
     4201 ggcgagcgca agctcgacca agctcgccga taatcagctc ctgagcgacc tgatccaggc
     4261 gctcggctgc ggcaccggcg cccgctatcg cgaggaggat ctgcgctacg agaaggtcgt
     4321 ggtgatgacg gacgccgatg tcgacggcgc ccatatcgcc tcgcttctca tcaccttctt
     4381 ctaccgcgag atgcgcgggc tgatcgaagg cggccatctc tatctcgccg tgccgccgct
     4441 ctatcgcctg agccagggcg ccaagacctt ctatgcgcgc aacgacgcgc ataaggacga
     4501 attgatgcgc aaggaattca acggcaaggg caaggtcgac gtctcacgct tcaaaggttt
     4561 gggcgagatg atgccgaagc agctcaaaga gaccaccatg gacccgaagg cgcgcattct
     4621 gcttcgggtc gagatcgccg acgacgacaa ggcgaccttc aagctggtcg atcagttgat
     4681 gggcaacaag cccgaggcgc gcttccgctt cattcaggag cgcgcggaat tcgccaagcc
     4741 cgacgcgctt gatatttaag ctagagccat cgcggcacgc gcgccttgta tttgcagtag
     4801 acgtcgccga acttccgctc gagatacgcc tcttcccgca ggacaacgcc gcgctgcaag
     4861 atcacgagca ccgggatcag caggagcagc acccacatgc tggtcaggac gatggcgaaa
     4921 ccgaaatagg cgagcacgaa accgatatag atcgggttcc gcgtgaagcg atagggtccg
     4981 gtggcgacca gagccgtcgc gggctcgctc ggcacgacgc tcgttctggc aaggcggaaa
     5041 ttgtggaccg aacgagccac aagcgcaaag ccgatgagga caaaagtcag gcccacggta
     5101 agcgcgagcg cattcgccga tccgagcccg ggcccgatcg gtatgaggaa gctcagcagc
     5161 gcgccggccg cgagcgcgcc aagaaacagg atcggcggca tgatcgcgac ttcggcatga
     5221 tcgttggcat tcatggtccg tcccgcccta tcgctcgcgc gttctggcga aaaagccgcg
     5281 catcctccag tcggcgcgca agcttaatgc ggcgccgtac cgggcacccg tgccaaattg
     5341 cctcttgcgc cgcgcgcgtt ggccgcttat ttgaggatca agcaatagca gggcataatc
     5401 ctcgcatcgc tcccgtcgta tcttgcacaa gggctgcgaa tcgttgcagc aaatatttgt
     5461 gcctaaaaga aagaaatacg cgcggcgcaa aagtcgcgcg gcaatttgac taacgcatga
     5521 gcttttctaa tcttggcctg agccccaaag ttttgtccgc cgtggaagcg gcgggcttcg
     5581 tgactccgac ccccattcaa gagaaggccg tccctgccgc catcgcgggc cgcgacgtgc
     5641 tcggcattgc gcagaccggg accggcaaga ccgcgtcctt cgtgctgccg atgctgaccc
     5701 ggctggagcg cggccgggcg cgggcgcgca tgccgcgctc gctgatcctg gagcccaccc
     5761 gcgagctcgc cgcgcaggtg gccgagcatt tcgacattct cgggaagagc cacaaactga
     5821 ccgtggcgct tctgatcggc ggcgtgtcct atgaggacca ggaccgcaaa ctcgatcgcg
     5881 gcgccgacgt cgtgatcgcc acgcccggcc gcctgctcga tcatttcgtt cgcggcaaac
     5941 tgctcttgaa cggcatcgag atcctggtgg tcgacgaggc tgatcgcatg ctcgacatgg
     6001 gcttcattcc cgacgtcgag cgcatctgca agcttctccc cttcacccgg caaaccttgt
     6061 tcttctcggc caccatgccg acggaaatcc agcgcctcgc cgacaagttc ctgcacaatc
     6121 ccgagcgcat tgaagtcgca ccgcaggcca ccgcggcggc gaccatcgtg caaaggctga
     6181 agaaggcgcc cgacgaggcg gcgggaaagc gcgaagcgct ccgagccttg atgcgggaga
     6241 tcgacgtcaa gaacgccatc atcttctgca accgcaagaa ggatgtcggc atcctccatc
     6301 gctcgctcca gcgccatggc ttcaatgtcg gcgccttgca cggggacttg gatcagcacc
     6361 agcgcacggc gacgctcgat gccttccgca gcggccaaat cgccttcctt gccgcgagcg
     6421 acgtggcggc gcgcgggctc gatatccccg aggtgagcca catcttcaat tacgacgtgc
     6481 cgatccagcc tgaggactat gtccatcgca tcgggcggac cggccgggcg ggacgcgaag
     6541 gcttcgccgc cacgctggtg acgccgcgag actacaaggc gctcaaggcg atcgagaaga
     6601 tgctgggcta cgagatcgcg tggatcgacg gcgcgccgag cgaggaagag cagaaagagg
     6661 ctctggactc gcggccgtac gggaatgggc gccatcgccg cggcggccgc tcaagacgcg
     6721 acaaacaccc ctcgggtccg cacgcgaaga cgggccatcc ctccagacct cacgggccga
     6781 acgtgcagcg gagcgctccg cagggctccc attacggcaa aaagcaagac gagcgccgcc
     6841 acgacaggcc gaagcatccc catcccggcg agcggaaatc agcccatggt cacgccaccc
     6901 ccgctcctca acagaagaag tcccagcccg agccgtccag ttcgcggcag cagggcatgg
     6961 gcgaccatgt tccggccttc atgatgcgcc cggtccgggt cacttaagcg cggcagagag
     7021 tatcagccta gagttccagc ccgcttcccc tctcccttga gggagaggac gaaacgagtg
     7081 tcattccggc gcaagccgga atacggggac aggcacgggc gcggacactg gataccggct
     7141 ttcgccggta tgacggagaa gggtagccca ggccgcaatc cagcccgtgt agcaccctat
     7201 ccctccccgc gcgggaaggg gcgaagccga gtgcaggcga ggctgagccg gggcggggtg
     7261 atcgtccggc acgttatctc ttcggcgcat tgtgcaacgg ccccctaccc cgacgccgcc
     7321 cttggcgtcg tcggacctcc ccacgagggg gaggtttaaa aagggcggag cgaaagtcgt
     7381 gagccggaat ccagaactgc gtgacgattg cgcgctgcat ccttcgaggg ccgcttcgcg
     7441 gccacctcag catgacgaat ccaggagagc gtacgcttat tctaacctcc ccctcgtgga
     7501 ggtccggcgt cgcgtttcgc gacgccgggg tggggggcgc gtctcccgcg gagagcgacg
     7561 ctgctttcag ggcgtcagac ggcgcttcgc gcctaacccc tcaccgcgcg ccgagcggcg
     7621 cgcgtgctct ccccaacctg cctgcgcgaa gccgaagcgg cttcgcctcg gcccgtccgc
     7681 cgaaggcttc gggccgcagg cgggcgaagg caggggggag agcggaatgc acctcaggat
     7741 gacgaaatcc taggagctgt cttgcgccga gtcctcggcg ggcttgagtg cgaccgactc
     7801 gccgcacccg caggcggatg tctggttcgg gttgttgaac acgaagccgg agcgcatgcg
     7861 atcggtgcga tagtccatct cggtgccgag caggaacatc accgcgctcg gatcgatcag
     7921 gagcttgacc cccttgtcct cgacaatctc gtcatgcggc ccgtgcgctt gcgcgtactc
     7981 catcgtgtat tccatgccgg cgcagccgcc gttcttcacg ccgatacgca cgccttcgac
     8041 cgtcttgctt gaagcggctt gaagggcgcg gatccgcgcg gcggcggcct cggtgaggcg
     8101 catcacttgg ggacggggac ggacggccat ggcttgaagt tggggcaaat cgggcgcgcg
     8161 ttcaaggctt gccgtcagaa catattgagg tcgagccgcg cctcgtcgct catgcggctc
     8221 gtgttccaag gcgggtcgaa cacgagattg accttgacct ggccaacgcc cgggacggcg
     8281 gagacggcgt tttcgaccca gcccggcatt tcgcccgcaa ccgggcagcc cggcgccgtc
     8341 agcgtcatgt cgatctcgat attgcggtcg tcgtccacgt ccagccgata gatgaggccg
     8401 agctcgtaaa tgtcgacggg gatctcggga tcgtagacgg tcttgagcgc ggcgatcagg
     8461 tcgcctttca gccgctcgac ctcgtcggga ggaagcgccg aaccagcccc ctccccgccg
     8521 ccggtcgcgc cgacgccagc cttgatctcg ccggactcgc cgccggcggc ctcaggctcg
     8581 gactgcggct cggcctcgcg ggccggggca tcgtcctttt gagttctttc aatcatcgcg
     8641 aaggcaacct taagcgaaca tctcgcgcgc cttgtgcagt ccctcgacca gagtatcgac
     8701 ctcttccatc gtattgtaca tggcgaagga cgcgcggcat gtcgaggtca cgccatagcg
     8761 ggcaaggagc ggctcggcgc aatgggttcc ggccctgacc gccactccat aacggtcgag
     8821 gatggtgctc acgtcgtggg gatgggcgcc ctccatattg aaggcgatga tgctcccttt
     8881 gtctttggcg cgcccgaaga tgcgcagcca gttgagctcg ccgaggcgct ccatggcata
     8941 gtcgaggagg gccgcctcgt gggccgcgat ccgctcgcgc cccacgctct ccacgtaatc
     9001 gatggcggcg ccgagcccga tggcctgcac gatggccggc gtgccggcct cgaaccggtg
     9061 cggcggcttg gcataggtga tcatatcagt ggtcacggtg ccgatcatct cgccgccgcc
     9121 ctgataggga cgcatgtgct ccaggtgctc tttcttgccg tagagcacgc cgatccccgt
     9181 cgggccgtag agcttgtgcc cggtgaacgc atagaaatcg atgtcgaggt cctgcacgtc
     9241 gacgggaaga tgcaccgctc cttgcgcgcc gtcggcaagg acacgaatgc cgcgcttgtg
     9301 cgcgatccgg accacctcct tcagaggcac gatggtgccg agcacgttgg acatatgggt
     9361 gatggcgatc atcttggtgc gcggagtgat cagccgctcg aacgcatcga ggagaaactc
     9421 gccgtcgtcg gagaccggcg cccatttcag aacagcgccc tggcgctcgc gcaagaaatg
     9481 ccagggcacg acattggagt ggtgctccat gatggtgaga aggatctcgt cgccttcgcc
     9541 gatatccggc gcgccgtagg aagacgcaat caggttgatc gcctcggtcg cgttgcgggt
     9601 aaagacgatc tcctcatcgg agcgcgcatt gaggaagcgg cgcacgcttt cgcgtgcctg
     9661 ctcgaaatgg gcggtggaaa gattgctgag atagtgcaac ccgcgatgca cgttggcgta
     9721 ttcgtcggcg taagcgcgca aggtcgtgtc gagcaccacg cgcggtttct gcgccgacgc
     9781 gccattgtcg agatagacga gctttttgcc gttcacttca cgagagagaa tcgggaaatc
     9841 ggcgcggatc gcctccacgt cgaacggggc cgccgccggt ctgcgatctt gcttcgtggg
     9901 agtgagcacg gtcatcggcc gcatcctcag tcggcagaaa tgccgagcca cgcggcggag
     9961 gcattgataa gcgcgtcgcg caaggcctcg tcctcgaccc gctcgatcgc ttcgccgacg
    10021 aaggcctgga cgaggagggc gcgggcttcc gcttcgggaa tgccgcgcgc ctcgagatag
    10081 aacagaagtt cgtcgtcgat ctggcccgag gtcgaaccgt ggccgcagac cacgtcgtcg
    10141 gcgaaaatct caagctcggg cttggaatcg aactcggcgg tttccgacag gagaagcgcg
    10201 cccgccatct gcttgccgtc ggtcttctgc gctccgggcg agacaatgat cttgccctgg
    10261 aagacgccgc gcgcctcgcc gtccagcaca cccttgaaca tctcgcggct cgtgcaatgc
    10321 ggcacgcggt gctcgacgag aagcgtcgtg tcgcaatgct gccggtcgcg gaggagaaag
    10381 gcgccggaaa tgtcggcagc cgccccttcg cccaaaaagc gcaaatagat ttgattgcgg
    10441 gcgagcgcgg ccccggtcga gaattggaag gcatcgtagc gggcatcacg accgagctcg
    10501 acaagccacg tgcaaaggtg gactgcgtcg tcgccgtcgc gctgcagctt gatgtgcttc
    10561 accgcggcct tggcgccgat cctgagctcc gtcgccgcgt tgcgctgtga tccgcggagc
    10621 ccgaggctgc cgaagctttc gaggatcgtg gcggagctgc cgggctcggc aatcacgacg
    10681 ttgcgggtga aggtcgaggc aggatcgcct gcgccgtcaa gcgcgatgac gtgtatcggc
    10741 ttgtccagga tcacgccctc gccgatgcgc aaggcgaggc cgcccgtcat cagcgccgtg
    10801 ttgagggcaa gcacggcgtc gtcgtcatgc ggattgacgt gaccaagggc ctttttgagc
    10861 caggccggcg gcttctcgag agcaaccgcc agcgagatcg cctcgacacc ggccgatttc
    10921 aggccggcga ggtccgagag atcggcgctc agatcgccct cgacgaggac gacacgatag
    10981 gcgggaaggc gcgcaatctc cgcgcccagc gcctggctca gagcaccctc gttcacggcc
    11041 gcgccgtcgg acttgccaag cggattgaca tcggtcagcc gggcgcggag gtcggtatat
    11101 ttccaggcct cgatgcggcg atggggcagg ccctgcccgc cgtaggccgc gatggccgtc
    11161 gtcctcaggt cggacacaaa gggatcgcgg gcgccgggaa gcgcattctc ggccgcctcg
    11221 aacaggtcga gataggcctg ttcggccttg gtcctgaatt gctggacggg gaggtccatg
    11281 acggcctttc ttacgcggct gcctcgccga actcggcgta gccggattcc tcgagctcaa
    11341 gcgcaagctc aggcccgccc gagcgcgcca ccctgccctc cgacagcacg tgcacgcggt
    11401 ccggcacgat gtagttcagg agccgctgat agtgggtgat gacgagcatc gcccgctcgg
    11461 gcgagcggag cgcgttcacg ccctccgcca cgatgcgcac ggcatcgatg tcgagaccgg
    11521 aatcggtctc gtcgagcacg cacagcgcag gctccaacag ggccatttgc aggatctcgt
    11581 tgcgcttctt ctcgccgccg gagaacccga cattgagcgg gcgcttcagc atctcgggct
    11641 cgatcttcag cgccttcgcc ttctcgcgca cgaagcgcaa gaaatcgggc gtcgaaagct
    11701 cggcctcgcc gcgcgctttc cgcacggcgt tcaccgccgt gcgcaggaag gtcatggtgg
    11761 cgacgcccgg aatctccatg gggtactgaa acgcgaggaa cacgcccgcg gcggcgcgtt
    11821 cgctcggctc catcgcaagc agatcctgtc ccttccaggt caccgagcct gcggtcacct
    11881 catactctgc cttgccggcg agcacatagg cgagcgtcga cttgcccgag ccgttaggtc
    11941 ccatgatcgc gtgcacttcg ccttgaggca gcgtgaggtc gaggccgttc aggatctcgc
    12001 ggccatcgac attgacgtgc aggttcttaa tttcaagcat tactcactag ctttcctaaa
    12061 ctgctctatg gccgcgatca gttctcccat ggaccgcttg tgtggggcac gataatccaa
    12121 atgattggta tcattgtgcg agcccttgaa ggtccaatcg tactgattgt tcgggtatgc
    12181 ccggacgtac atagggacac cgtcgtcacg cctaaaaaac agctctccac tgcggaggat
    12241 cgaataggcg attcctagtt tgtccaaacg ttcgcaaaca gacttcatga tctgctcggc
    12301 tcctttgtcc ctatttgcag atcggtttcc cgaatcgtga ttctgagtca cggtttcgct
    12361 gatgccgctt gtgacagaaa ccccctgtcg caacctggtt gagatcctcc ggatcaactc
    12421 gtccttgggc ggaagtttcg tattagtcat gcccagcaat ctggctatcc tctgcaattc
    12481 tggtttcacc gtgaaaactc gcagttggcc ttctatttgt tcacctctta agagtctctc
    12541 tttgagccga ttgatcactt cccctgcgcc ttgctcatca atccgctgac gtggcttctt
    12601 aggtacagga atccgttgtg gcagagcagc tttgagttgt cgctcaatcc gcagcagttg
    12661 ctgggatgct tgtcgcagca atgccttgac ttcggcgatg tcgtcactga cgagcagaat
    12721 cgcctcttcg tcagtttttg ttgagtc
//
LOCUS       DASUQP010000233         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1748889, whole genome shotgun sequence.
ACCESSION   DASUQP010000233 DASUQP010000000
VERSION     DASUQP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748889"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFY74_10820"
     CDS             <1..484
                     /locus_tag="VFY74_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5932222.1"
                     /translation="NEQTRVRRMEVGFVYQFHHLLPEFSALENVVLPQLILGSSRDQA
                     ETRAKDLLKSLGLEERWDHRPAQLSGGEQQRVAIARAVANAPKVLLADEPTGNLDPHT
                     AERVFEQLLKLVRQSGVAAVVATHNLDLAARMDRTLRLMEGRLVEEEVVVARAEVARC
                     "
     gene            655..>3392
                     /gene="dnaE"
                     /locus_tag="VFY74_10825"
     CDS             655..>3392
                     /gene="dnaE"
                     /locus_tag="VFY74_10825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5932223.1"
                     /translation="MASQDKPFVHLRVHSAYSLLEGALTIPKLAALARADGAPALALT
                     DTDNLFGALEFSEALAEAGVQPIIGMTLSLTFGGRSASALPAGEDHRPRAEGRIALLA
                     KDAQGYANLMHLSTEAYFAASETGEVVTAIGDLAARAEGLIALSGGFEGPIDRSLAEG
                     STELARARAEELKRIFGDRLYIELQRHGLPQERAVEPLLLKLAYDLDLPIVATNEAYF
                     ASADDFEAHDALLCIAEGSYVGVDDRRRLSPEHYFKTGEEMRRLFADLPETLDNTIEI
                     AHRCAYRPLQRQPILPPFVTAAADAKARAETEAAELRRQSEQGLRGRIAQAGVAPGYD
                     EAAYAERLAFELDVITSMNYQGYFLIVADFIKWAKAEGIPVGPGRGSGAGSLVAYALT
                     ITDLDPIRFGLLFERFLNPERVSMPDFDIDFCQDRRDEVIAYVRERYGAHRVAHIITF
                     GKLQARAVLRDVGRVLQMPYGQVDRLCKLVPMNPANPVTLPQAIAAEPRLQEARDAEP
                     IVAKLLDVGQRLEGLYRHASTHAAGVVIADRPLIDLVPLYRDARAQLPATQFSMKWAE
                     AAGLVKFDFLGLKTLTVIDTARKLIARRGIDIDPGTLPLDDAATYGLLQKGDTVGVFQ
                     LEGQGMRDALRKLKPDRFEDIIAIVALYRPGPMDNIDSYVNRKHGREGIETLHPMIEP
                     ILKETYGVIIYQEQVMQIAQVLSGFSLGEADLLRRAMGKKIKKEMAAQKSRFIEGAVA
                     NGVDRGRAEYIFELVAKFAGYGFNKSHAAAYALIAYQTAYLKANYPSEFLAASMTLDM
                     SNADKLSGFAQEARRLGMRLEPPSINRSEVEFVPSDGAIRYSLAALKNVGRQAVEHLT
                     EERKERGPFRDVSDFARRINPRLMNKRALETLAAAGALDELRIDRATAFANVDRIIA"
BASE COUNT          614 a         1110 c         1089 g          579 t
ORIGIN      
        1 caacgagcaa acccgcgtgc gccgcatgga ggtgggcttc gtctatcagt ttcatcactt
       61 gctgcccgag ttttcggcgc tcgagaatgt ggtgctgccg cagctcatcc ttggatcgag
      121 ccgcgaccaa gccgagacca gggccaagga cctgctgaag tcgctcggtc tcgaagagcg
      181 ctgggatcat cgcccggcgc agctctccgg cggcgagcag cagcgcgtgg ccattgcccg
      241 cgccgtcgcc aacgctccca aagtgctgct cgccgacgaa ccgaccggca atctcgatcc
      301 gcataccgcg gaacgcgtgt tcgagcagct gttgaagctc gtgcggcagt cgggcgtggc
      361 ggcggtcgtt gccacgcata atctcgatct tgccgcacgc atggatcgca cgcttcgcct
      421 catggaaggc cgcctcgtcg aggaagaagt ggtggtggcg cgggcggagg tcgcgcgctg
      481 ctagccccag ccgtcttgat tagaacaaaa gaagaacata aattggtttc agggcgcgca
      541 gcgagtcctc ggaaagcggg gataaggcgg cgaccgggcc ggcacagcct tcgatgcggc
      601 cctcgaatcc tccaggctgg gcgcggcagg cggctagaag cgggacggtg aggcatggcg
      661 agccaggata agcctttcgt gcatcttcgc gtgcactcgg cctattcgct gctcgagggc
      721 gcgctcacca ttccgaaatt ggcggcgttg gcgcgcgccg atggtgcgcc cgcgctcgcc
      781 ctgaccgaca ccgacaatct cttcggcgcg ctcgaattct ccgaggcgct tgccgaggcg
      841 ggagtccagc ccatcatcgg catgacgctg tcgctgacct tcggtggaag gtccgcaagc
      901 gcgctcccgg caggcgagga tcaccggccg cgggccgagg gacggatcgc gctcctcgcc
      961 aaggacgctc aaggctatgc caacctcatg cacctctcga ccgaggccta tttcgccgcg
     1021 agcgagacgg gggaggtcgt cacggccatc ggcgatcttg cggcaagagc ggaaggtctc
     1081 atcgcgctga gcggcggatt tgaaggaccg atcgatagga gcctcgccga aggcagtacc
     1141 gagcttgcgc gcgcacgggc ggaggagctc aagcgcattt tcggcgaccg gctctatatc
     1201 gagctgcagc gtcacgggct gccgcaggag cgggcggtcg aacctcttct gctcaagctc
     1261 gcctatgacc tcgacctgcc gatcgttgcg accaacgaag cctattttgc cagcgcggac
     1321 gatttcgaag cgcatgacgc gctgctctgt atcgccgaag gaagctatgt cggcgtcgat
     1381 gaccgtcgcc gcctgagccc cgagcattac ttcaagaccg gcgaggagat gcggcggctc
     1441 ttcgccgatc tgcccgaaac tctcgacaac accatcgaga tcgcccaccg ctgcgcctac
     1501 cggcctctcc agcgccagcc gatcctcccg ccattcgtga cggccgccgc ggatgccaag
     1561 gcgcgcgccg agaccgaagc ggcggagctc aggcgccagt ccgaacaggg tctaagaggc
     1621 cggatcgctc aagctggcgt ggcgccgggc tatgacgagg ccgcttatgc cgagcggctc
     1681 gccttcgagc tcgatgtcat caccagcatg aactatcagg gctacttttt gattgtcgcc
     1741 gacttcatca aatgggcgaa ggccgaaggc attccggtcg gacccggccg cggctcgggc
     1801 gcgggctcgc ttgtcgccta tgcgctgacc atcaccgatc tcgacccgat ccgcttcggg
     1861 ctgctgttcg agcgcttctt gaatccggag cgcgtgtcga tgcccgattt cgacatcgac
     1921 ttctgtcagg accggcgcga tgaggtgatt gcctatgtgc gcgagcgcta cggcgcgcat
     1981 cgcgtcgccc atatcatcac cttcggcaag ctccaggcgc gcgcggtgct gcgcgacgtg
     2041 ggccgcgtgt tgcagatgcc ctacggccag gtcgaccggc tgtgcaagct cgtgccgatg
     2101 aaccccgcca atcccgtcac gttaccgcag gcgatcgccg cagagccgcg gctccaggag
     2161 gcgcgcgacg ccgaaccgat cgtggcgaag ctgctcgatg tcggccagcg cctcgaaggc
     2221 ctctatcgcc atgcctcgac tcatgccgcg ggcgtcgtca tcgccgaccg tcccctgatc
     2281 gacctcgtgc cgctctaccg cgatgcccgc gcgcaattgc ccgcgaccca gttcagcatg
     2341 aagtgggcgg aagccgcagg actggtgaag ttcgactttc tcggcctcaa gaccctcacc
     2401 gtgatcgata cggcgcggaa gctgatcgcg cggcggggga tcgacatcga tccgggcacg
     2461 ctgcctctcg acgacgccgc gacttacggc cttctgcaaa agggcgacac ggtcggcgtg
     2521 ttccagctcg aaggtcaggg catgcgcgat gcgctcagga agctcaagcc cgaccgcttc
     2581 gaggacatca tcgcgatcgt ggcgctctac cggccaggac cgatggacaa cattgattcc
     2641 tacgtgaacc ggaagcacgg gcgtgaaggt atcgagacgc ttcatcccat gatcgagccg
     2701 attctgaaag agacctacgg cgtcatcatc taccaggagc aggtgatgca gatcgcgcaa
     2761 gtgctctccg gcttctcgct cggcgaagcc gatctgctcc gccgcgccat gggtaagaag
     2821 atcaagaagg agatggcggc gcaaaagtcc cgctttatcg agggcgcggt cgcaaatggc
     2881 gtggaccgcg gccgcgccga atacatcttc gagcttgtgg caaagtttgc cggctacggt
     2941 ttcaataaat cgcacgccgc ggcctacgcc ttgatcgcct atcagacggc gtatctcaag
     3001 gccaactacc cgagcgagtt cctggcggcg tcgatgacgc tcgacatgag caacgccgac
     3061 aagctgagcg gcttcgccca agaggcgcgg cggcttggca tgaggctcga gccaccctcg
     3121 atcaaccgct cggaagtgga attcgtcccg agcgacggcg cgatccgata ttcgctcgcg
     3181 gcgctgaaga atgtcggccg ccaggcggtg gagcatctca ccgaggagcg gaaggagcgg
     3241 ggaccgttcc gcgacgtctc ggatttcgcc cgccgcatca acccgcgtct catgaacaag
     3301 cgggcccttg agacgctggc ggcagccggc gccctcgatg agcttcggat cgatcgcgcc
     3361 acggccttcg ccaatgtcga tcgcatcatc gc
//
LOCUS       DASUQP010000234         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1476199, whole genome shotgun sequence.
ACCESSION   DASUQP010000234 DASUQP010000000
VERSION     DASUQP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476199"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFY74_10830"
     CDS             complement(<1..202)
                     /locus_tag="VFY74_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020811982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX5932224.1"
                     /translation="MSIAFIFPGQGSQAVGMGAELAKAYPSARAVFEEVDAALAQKLS
                     RVMWEGPESELTLTENAQPALMA"
     gene            419..898
                     /locus_tag="VFY74_10835"
     CDS             419..898
                     /locus_tag="VFY74_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932225.1"
                     /translation="MKRPALLGTLVAAIGIASAGGSLLAADPQPWPWKSKLPPDPALV
                     VLTSVEDVTAAINKSDPPTVTVTVNASAPTPNYSELQLTPRMGDPKDLIFAFDAKGRP
                     PQDLSTQVVTPVSFTVEYVDAPIDKVGVVEVHAQSGCKAFSLTENKAVACTSVSASQ"
     gene            1130..1612
                     /gene="rpsF"
                     /locus_tag="VFY74_10840"
     CDS             1130..1612
                     /gene="rpsF"
                     /locus_tag="VFY74_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5932226.1"
                     /translation="MPLYEHVFLARQDISAQQVDSLLQGFRSILEERGGSVGKTEYWG
                     LKSLTYRIKKYRKAHYVLMNIDAPHEAVVEIERQMRLSTDIIRFLTVRVDEHDPAPSP
                     MMRRADRDEREGRDRDRGDRDRGDRRGGRRDRDFAPREARGGGDADVEETKEGEEDAT
                     "
     gene            1615..1854
                     /gene="rpsR"
                     /locus_tag="VFY74_10845"
     CDS             1615..1854
                     /gene="rpsR"
                     /locus_tag="VFY74_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5932227.1"
                     /translation="MTQVQARRPFYRRRKTCPFSGDKAPKIDYKDVKLLQRYVSERGK
                     MVPSRITAVSAKKQRELARAIKRARFLGLLPYLIQ"
     gene            1953..2930
                     /locus_tag="VFY74_10850"
     CDS             1953..2930
                     /locus_tag="VFY74_10850"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX5932228.1"
                     /translation="MPTPLVIGAGSGLVAAALFASAATATALAGVLFYLAPLPLCLAG
                     LGWGSTAALLAALTGTIVVALSFGPAMAAVFAASIAAPIALLTYLALLSRPAPAPEGQ
                     SSGALDWYPPGRLVGWAALIAGLLAALLVLFLGSDQDSYRESIREILSHSALKDLDRD
                     GTLFTEENIAKLSGLIARALPAAFAVVWLTITLFNLWIAGIIVEASGHALRPWPDLNA
                     LEIPNAFLLIFAAALAASFLPGLPGLLATGLAGALLFAYTLQGLAVIHVYSRGMPLRG
                     LLLATVYLAILLLGWVAIVVAIIGLAEPTLGLRQRAARGGQPPKSDGTI"
     gene            2951..3559
                     /gene="rplI"
                     /locus_tag="VFY74_10855"
     CDS             2951..3559
                     /gene="rplI"
                     /locus_tag="VFY74_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5932229.1"
                     /translation="MQVILLERIGRLGQMGDVVTVKDGFARNYLLPQGKALRATEANR
                     KRFERDRAQLEARDLELKSEAQAVAAKLDRQSFIVIRQAGDTGQLYGSVSTRDIAEAV
                     TEGGFTIERRQVALDRPIKTLGLHQIRLQLHAEVEPHITVNVARSPDEAARQARGEDV
                     TRAVTQAEEDAEAARRAAAALFEEGAPAEIVGEATEGEQGGA"
     gene            3645..>4070
                     /locus_tag="VFY74_10860"
     CDS             3645..>4070
                     /locus_tag="VFY74_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5932230.1"
                     /translation="MSALIRPVAPETPIFREAPHNLEAEQALLGAILVNNEAIDRVSN
                     FLKPEHFHDQLHARIYETAAKLILSGKRATPITLKTFFQAEPASGELSIPQYLGRLAA
                     NATTIINAEDYGRTVYDLAIRRQLIQIGETMVNTAFDSPI"
BASE COUNT          698 a         1393 c         1250 g          729 t
ORIGIN      
        1 ccgccatcag cgccggctgc gcgttctcgg tgagcgtcag ttcgctttcg ggaccctccc
       61 acatcacgcg cgacagtttt tgcgccagcg ccgcgtcgac ttcctcgaac acggcccgtg
      121 ctgaagggta agccttggca agctcggctc ccatgccgac agcctggctt ccctgcccgg
      181 gaaagatgaa agcaatgctc atgatggctg gatggagggg cccgcggtcg ggccaaagac
      241 accgtttcat ttctttctgt caagcgaggc ttgcggcggc cggtcggtcg cttcgcgcct
      301 ttcgctcggc cgaaagtcag cccacgaacc gaaaagggag cgcgggcctg caacgccccg
      361 tgctagaaca gcgtttggac gcaaatcaca gctcaagatt cgtacacgga gaaaatcgat
      421 gaaacgcccc gctctcctcg gcaccctggt cgcagcgatc ggaatcgcga gcgccggcgg
      481 ctcccttctc gccgccgacc cccagccatg gccgtggaag tccaaactcc cacccgatcc
      541 cgcgctcgtc gtcctgacct cggtcgagga cgtgaccgcc gccatcaaca aatccgatcc
      601 gcccacggtg accgtgacgg ttaacgcttc ggcgcccacc ccgaactaca gcgagcttca
      661 actcacgccg cgcatgggcg atcccaagga ccttatcttt gccttcgacg ccaagggacg
      721 gccgccccag gatctcagca cgcaagtggt caccccggtg agtttcaccg tcgaatacgt
      781 cgatgcgccg atcgataagg tcggggtggt cgaggtccat gcgcaaagcg gctgcaaggc
      841 gttttcgctg accgagaaca aggccgttgc ctgtacctcg gtgtcggcct ctcaatagag
      901 agcagccttc gccgggtcgg cccggctctt gcaaacggag agcgaagtcc tataagaccg
      961 gcaccagcaa ccgctccggt cggaggctgg acggaggacc gctcgcgaga gcggtaggct
     1021 tcaagccgcc ctcccgtgtc tccgctctct gggtcgtctc ttgggccttt ccttctaaag
     1081 cgcccatggg aggcttcgca ccggagcaac gacgaaggaa aggctatcca tgcctcttta
     1141 cgagcatgta tttctggcgc ggcaggatat ttccgcccag caggtcgact ccctccttca
     1201 gggttttcgc tccattctgg aggagcgcgg agggtcggtc ggcaagaccg aatattgggg
     1261 cctgaagtcc ctcacctatc gcatcaagaa gtaccgcaag gctcactatg tgctgatgaa
     1321 catcgacgcc ccgcacgagg cggtggtcga gatcgagcgt cagatgcgcc tttcgaccga
     1381 catcatacgc ttcctcaccg tgcgcgttga cgagcatgac ccggcgccgt cgccgatgat
     1441 gcgacgggcc gaccgcgacg agcgcgaggg ccgtgatcgg gatcgcggtg atcgggatcg
     1501 cggcgatcgc cgcggaggcc gacgcgaccg tgacttcgcc ccgcgggagg cgcgcggcgg
     1561 gggtgatgcg gacgtcgagg agacgaaaga aggtgaggag gacgcaacat gagcatgacc
     1621 caagtccagg cgcgccgccc cttctaccgc cggcgcaaga cctgcccgtt ctccggggac
     1681 aaggcgccga agatcgatta caaggacgtg aaactgctgc agcgctatgt ttcggagcgg
     1741 ggcaagatgg tgccgagccg catcacggcg gtatcggcga agaagcagcg cgagctcgcc
     1801 cgggcgatca agcgcgcgcg gttccttggc ctcctgccct acctgattca ataggcggtt
     1861 cgggcgcata gccgcgctct gggcttaaca gaaagttggg gtgggacacg ccctccccta
     1921 accgctttcg aaggaggcgg gacagcgtat cgatgccaac acctctcgtc atcggcgccg
     1981 gttcaggtct cgtagcggcg gcgttgttcg cctcggccgc gaccgccacg gctcttgccg
     2041 gcgtgctctt ttatcttgcg ccgcttccgc tctgtcttgc cgggctcggc tggggcagca
     2101 cggccgcact gcttgcggcg ctgaccggca cgattgtcgt ggcgctgagc ttcgggccgg
     2161 ccatggcggc ggtgttcgca gcttcgattg ccgcgccgat cgcgcttctc acctatcttg
     2221 cgctgctctc gcgccccgca ccggccccgg agggtcaaag cagcggcgcg ctcgactggt
     2281 atccgcccgg gcgtctcgtc ggctgggcgg cgctcatcgc cggcctgctc gccgctctcc
     2341 tcgtgctctt cctcggttcc gaccaggaca gctatcggga gtcgatccgc gagatcctga
     2401 gccacagcgc tctcaaggat ctcgaccgcg acggcacgct cttcaccgag gagaacatcg
     2461 ccaaactttc gggactgatc gcccgggcgc ttcccgccgc tttcgccgtg gtctggctca
     2521 ccatcaccct gttcaatctc tggattgccg ggatcattgt cgaggcgtcg ggacacgcgc
     2581 ttaggccctg gcccgacctt aatgccctgg aaattccgaa tgcgtttctc ttgatcttcg
     2641 ccgccgcact cgcggcgtcc tttctgccgg gcctgcccgg attgctcgcg accggtctcg
     2701 ccggcgcgct cctcttcgcc tacacgctcc agggattggc ggtcatccac gtctatagcc
     2761 gcggcatgcc gcttcgcggc cttctgctcg cgaccgtcta cctcgcgatt ttgctgctgg
     2821 gatgggttgc catcgtcgtt gccattatcg gtttggccga gccgacgttg ggcttgcgcc
     2881 agcgggccgc gcgcggcggg cagccgccca agagcgacgg aacgatctga caaggatagg
     2941 agtgtcgaac atgcaagtga ttcttcttga gcgcattgga cgcctcggac agatgggcga
     3001 cgtcgtcacc gtcaaggacg gttttgcgcg caactatttg ctgccccaag gcaaggcgct
     3061 tcgtgccacc gaagccaatc gcaagcgctt cgagcgcgat cgcgcccaac tcgaagcccg
     3121 cgacctcgag ctgaaatccg aggctcaggc cgtggcggcc aagctcgatc gccagagctt
     3181 catcgtgatc cgccaggccg gcgacaccgg tcagctctat ggctcggttt cgacccgtga
     3241 catcgccgaa gccgtgaccg agggcggctt caccatcgag cggcggcagg tcgcgctcga
     3301 ccggccgatc aagacgctcg gcctccacca gatccgcctt cagctccacg ccgaggtcga
     3361 acctcacatc accgtcaatg tggcccgttc gcccgacgaa gcggcgcggc aggcccgcgg
     3421 cgaagacgtg acccgcgcgg tgacccaggc cgaggaggat gcggaagccg cacggcgtgc
     3481 cgcggccgcg ctgttcgaag agggtgcccc ggccgagatc gtgggcgagg cgacagaagg
     3541 cgaacaggga ggcgcctaac cgctccttgt gggcatccca cgataccccc gctgttcaca
     3601 gacccggcat cttccagccc tgttcctttg ggctagtttg atccatgagc gcactgattc
     3661 gcccggttgc cccggagacg ccgatcttcc gcgaggcgcc tcacaatctc gaggcggagc
     3721 aggcccttct cggcgcaatc ctcgtgaaca acgaggcgat cgaccgcgtc tccaatttcc
     3781 tcaagcccga gcatttccat gaccagctgc acgcccgcat ctatgagacc gcggccaagc
     3841 tgatcctgag cggcaagcgc gcgaccccga tcacgctcaa gacctttttc caggccgagc
     3901 ccgcttccgg cgagctgtcg atcccgcaat atctcgggcg tctggcggcc aacgcgacca
     3961 ccatcatcaa cgccgaggat tacggccgca ccgtctacga tctcgccatc cgccgccagc
     4021 tcattcagat cggcgagacg atggtcaaca ccgccttcga ctcgcccatc
//
LOCUS       DASUQP010000235         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_252670,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000235 DASUQP010000000
VERSION     DASUQP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252670"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VFY74_10865"
     CDS             complement(<1..1542)
                     /locus_tag="VFY74_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5932231.1"
                     /translation="MQSNQAPNAPPSPENSKAPLPPRPAFPAMSRRTRLVLIAAGTVV
                     GAALLTLFAVNLLISADWVRDRVANRIKEQTGRELTVNGTTSLLFTPGPHVVITDATF
                     TDPEERAGTTDVSVGRLLIDLSLIELMSRNVDSERVVLERPVVTVRLGDDGRKAEPKK
                     PKTAKAPNPNPKPRRDVKLKDVRIEDGTVNIVYGGTGKERRIEHITGNLSLPTLTAPF
                     TGSGKFDWKDQTVNFSFELTSVADLREKRPARLLVALETPALAARFDGSLITRPDLSG
                     QGELSAKAHSIPSLLAWMREKPASVTGIGDGELASHVAWKDGEITFSNSRFALEHAKG
                     QGQAVVTLRAPRPHIRAALALEHLDLNPFLASGPKAAGADRNRSQSEPRAAPQTEKTP
                     ADAEGAKDWFTKPDAGQSAGASEGAQEPAPSASAGQPAETASTAPLAAPVARAPAADA
                     APASFDADVNLNVRKTRVFHLELGPSSLGLAFRDGVLNATLGGMELYDGHASGKLVLD
                     ASKPVP"
     gene            complement(1632..2723)
                     /locus_tag="VFY74_10870"
     CDS             complement(1632..2723)
                     /locus_tag="VFY74_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HEX5932232.1"
                     /translation="MKLGRRALLAGLAGGAAMVSLPGRASRALEPELFAASRTDDRGN
                     AQAAVFTLAGDEQTVELPGRGHDIALAPGGGTWVMFARRPGRFGVAVSLGAEPIWFAA
                     KPGRHFFGHGAFSSDGRLLYSTENDYERAAGMIGVRDATGGYKQIGEFSAHGMEPHDI
                     ALLADDRTMVIANGGIRTHPDHGADELNLAEMQPSLMYVDAATGDLLEEHRLAPALHQ
                     LSIRHLAAGANDTVIFGCQYRGPEEDTPALIGFHRRGETPVIVEAPAGMQKALRNYIG
                     SVTADRSGSIVAASAPRGNQVTYWDVAGRRYLGANHLMDGCGLAPTHRSASFLLTSGE
                     GWLVTAGPGIAISRQASRFQWDNHAILVR"
     gene            complement(2727..3758)
                     /locus_tag="VFY74_10875"
     CDS             complement(2727..3758)
                     /locus_tag="VFY74_10875"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEX5932233.1"
                     /translation="MSAQAEADHGTIARAALREVIRPGYAAFAEEAAYLNETVGALCR
                     TLSAASLDASKRAFAAAVAAWSKVEILRFGPITDDRRYERLFYWPDPKGLGPKQVQDA
                     LAKHDESVTGPEALAGKSVALQGLPALEYLLFGDGAEALAAKAGESAFRCGFAHAVAA
                     NIANIAKSVVEDWREGSPYEKAFLAPDPQGSVYRAPKEVTLDLFKSFTAGIELVRDQK
                     LARPLGASLEEARPRLAAFWRSGLSFANAAGNLEGVRTLFAKGGFAEVVAEDSPGVEK
                     SVMFDLGRAIEILGAIREPMADVVKDQALRGKIEALRVSLKSARQTAADAVARGAGLA
                     FGFNAMDGD"
     gene            complement(3811..>4581)
                     /locus_tag="VFY74_10880"
     CDS             complement(3811..>4581)
                     /locus_tag="VFY74_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX5932234.1"
                     /translation="VPEEQILANADPDDADMDGISGKPNRVWSREDEKAMLGRFGWKA
                     GVPSIAQQTAEAAGGDIGLSTVMSPQGAGDCTEKQKACFEAPNGNSPKYQDVELGDEL
                     FKLVTFYAQNLAVPARRNPDDPEVLKGKAIFHAIGCASCHRPRFVTGKVPGQPHLSHQ
                     LIWPYTDLLLHDMGEGLSDGRPEGEAAASEWRTPPLWGIGLTKTVSGHTLFLHDGRAR
                     NLTEAVLWHGGEGQPARDKFAGLSKADRDALLAFVNSL"
BASE COUNT          751 a         1483 c         1542 g          805 t
ORIGIN      
        1 gggaaccggc ttggacgcgt cgagcacgag cttgccgctt gcgtgtccgt cataaagctc
       61 catgccgccg agcgtcgcgt tgagcacccc gtcgcgaaag gcaaggccga gcgagcttgg
      121 gccgagctcg agatggaaga cgcgcgtttt ccgcacgttg agattgacgt cggcgtcgaa
      181 ggaggccgga gcggcatccg ccgccggcgc tcgcgcgacg ggagcggcaa gcggagctgt
      241 gctcgcggtt tcggcgggct gcccggcaga agcactgggc gcgggctctt gcgcgccttc
      301 gctcgcgcct gcgctttggc cggcgtccgg cttggtgaac cagtccttcg cgccttcggc
      361 gtctgccggc gtcttctcgg tttgaggggc tgcacgcggc tcggactgag agcgattgcg
      421 gtccgcgccc gcagccttcg gacctgacgc caggaacgga ttgagatcga gatgttcgag
      481 cgcaagcgcg gctcggatgt gcggtcgcgg cgcgcgcaag gtgacgacgg cctgtccctg
      541 tcccttcgca tgttcgagtg caaagcgcga gttgctgaaa gtgatctcgc catccttcca
      601 ggcgacatgg cttgcaagct cgccgtcgcc gatgccggtc acggaagccg gtttctcacg
      661 catccaggcc agcagcgagg gaatcgagtg cgccttggcc gacaactcgc cctggcccga
      721 gaggtccggc cgggtgatga ggctgccatc gaaccgcgcg gcaagagccg gggtttcgag
      781 cgcaaccaag aggcgcgccg ggcgcttctc gcggagatcg gcgacggagg tcagctcgaa
      841 actgaagttg accgtctggt ctttccaatc gaacttgccg gatcctgtga agggggccgt
      901 gagcgtcggc aggctgagat tccccgtgat gtgctcgatg cgcctctctt tgcctgtgcc
      961 cccgtagacg atgttgacgg ttccgtcctc gatgcgcacg tccttgagct tgacgtcgcg
     1021 gcgcggcttg ggattgggat ttggcgcctt ggccgttttc ggtttcttcg gctcggcctt
     1081 cctgccgtca tcgccgaggc ggaccgttac caccggccgc tccaggacga cgcgctcgga
     1141 atcgacgttg cggctcatca gctctatcag gctgagatcg atcaggagac gcccgaccga
     1201 gacatcggtc gtgccggcgc gttcctcggg atcggtgaag gtcgcgtcgg tgatgacgac
     1261 atgaggccca ggcgtgaaga ggagcgaggt cgtgccgttg acggtaagct cccgccccgt
     1321 ctgctccttg atgcgattgg ccacgcggtc gcgcacccaa tccgccgaga tgagaagatt
     1381 gacggcgaaa agcgtgagca aggccgcgcc gacgacggtg ccggccgcga tcagcacgag
     1441 ccgcgtgcgg cggctcatgg ccggaaaggc cggacgcggc ggcaagggcg ccttgctgtt
     1501 ctccggcgac ggcggtgcgt tgggagcctg gttgctttgc atgagctcgc gatcgaatcc
     1561 ccgattctag cccgacaaat aaggtgagat taggaattgg gatttgcctt gaaggagcgg
     1621 gtggtcgcac gtcaacgcac caggatcgcg tgattatccc actgaaatcg cgaagcctgg
     1681 cgcgatatgg cgatccccgg accggcggtg acaagccagc cctcgccgct cgtcaaaaga
     1741 aagcttgcgc tccgatgtgt gggcgcaagc ccgcagccgt ccatcagatg gtttgcgccg
     1801 agatagcgcc ggcctgcgac gtcccaatag gtgacttggt tgccccttgg cgcagaggcc
     1861 gcaacgatac tgccgctccg gtcggcggta accgagccga tatagttgcg caaagccttc
     1921 tgcatccccg ccggcgcttc gacgatgacc ggagtctcgc ctctgcgatg aaagccgatc
     1981 agcgcaggcg tatcctcctc ggggcctcga tattggcacc cgaagatgac ggtgtcgttc
     2041 gcgccggccg cgagatggcg gatcgagagt tgatgcagcg cgggcgccag gcgatgctcc
     2101 tcgaggagat cgccggtcgc cgcatcgaca tacatgagcg agggctgcat ctcggcgaga
     2161 ttgagttcat ccgcgccgtg gtcgggatgg gtgcggatgc cgccattggc gatcaccatg
     2221 gtgcgatcgt cggcaagaag cgcgatgtcg tggggctcca tcccgtgcgc cgaaaactcg
     2281 ccgatctgct tgtagccgcc ggtcgcgtcc cgcaccccga tcatgccggc ggcgcgttcg
     2341 taatcgttct ccgtggaata gagcagcctg ccgtctgaag agaaggcgcc gtggccgaag
     2401 aaatgacgtc ccggcttcgc cgcgaaccag atcggctccg cgccgagcga cacagcaaca
     2461 ccgaaccggc cgggtcgccg cgcaaacatg acccacgtgc cgccgccggg tgcgagcgcg
     2521 atatcgtggc cgcgtccggg caactcgacc gtctgctcgt cgcccgcgag ggtgaaaacg
     2581 gctgcctggg cgtttcccct gtcgtccgtg cgggaggccg cgaagagctc aggctcaagc
     2641 gcccggcttg cgcgaccggg aagcgagacc atagctgcgc caccggcgag accggcgaga
     2701 agcgcgcggc ggccgagctt catggcctag tctccatcca tggcattgaa gccgaaggcg
     2761 aggcctgcgc cgcgcgcgac cgcatcggcg gcggtctgac gcgcgctctt cagagagacg
     2821 cgcagcgcct cgatcttgcc gcgcaatgcc tggtccttga ccacgtcagc catcggctcg
     2881 cggatcgcgc ccaagatctc gatggcgcgc ccgaggtcga acattacaga tttctccacg
     2941 ccgggcgaat cttccgccac gacttccgca aagccgcctt tggcgaagag cgtgcgaacg
     3001 ccctcgagat tgccggcggc gttggcgaaa ctgaggccgc tcctccagaa cgccgccagc
     3061 ctgggtctcg cctcctccag cgaggcgccg agcggcctcg cgagcttctg gtcgcggacg
     3121 agttcgatgc ccgccgtgaa ggacttgaac aggtcgagcg tgacctcttt cggcgcacgg
     3181 tagaccgaac cttggggatc gggtgcgaga aaggcctttt cataaggcga gccttcgcgc
     3241 cagtcttcca ccaccgattt ggcgatgttc gcgatattgg cggcgacggc gtgagcgaag
     3301 ccgcagcgaa aagcgctctc acctgcttta gcagcgagcg cttccgcgcc gtcaccgaag
     3361 agcagatatt cgagggcggg aagaccctgc aaggccacgc tcttgccggc gagcgcctcg
     3421 ggccctgtca cgctctcatc gtgtttggcg agcgcgtcct gaacctgctt tggtccgagt
     3481 ccctttggat cgggccagta gaacaatcgt tcatagcggc gatcgtccgt gatcgggccg
     3541 aagcgcagga tctcgacctt gctccatgcc gcgaccgccg cggcgaaggc gcgcttgctt
     3601 gcgtcaaggg aagctgccga cagcgtgcgg caaagcgcgc cgaccgtctc gttgaggtag
     3661 gcggcctctt ctgcaaaggc ggcgtagcct gggcgaatga cttcgcgcaa cgcggcgcgg
     3721 gcgattgtcc cgtgatcggc ttcggcctga gcgctcaccg cgctcgcaca tacgctctca
     3781 cacaaagcag gacggccgcg aacccgcgcc tcatagcgaa ttcacgaagg cgagcagcgc
     3841 atcgcggtcg gccttggaca gcccggcgaa cttgtcgcgc gctggctgcc cctcgccgcc
     3901 gtgccagaga acggcctcgg tgagatttcg cgcccgcccg tcatgcagaa agagcgtatg
     3961 cccgctcacg gtcttcgtga gaccaatgcc ccagagcggc ggcgtccgcc attcgcttgc
     4021 agccgcctcg ccttcgggcc tgccatcgga aaggccctcg cccatatcgt gcagcaggag
     4081 atcggtgtaa ggccagatca gctgatgcga gagatggggc tggccgggca cctttccggt
     4141 cacgaatctc ggccggtgac agctcgcgca gccgatggcg tggaagatcg ccttgccctt
     4201 cagcacctcc gggtcgtccg gattgcgccg cgcgggcacg gcgaggttct gcgcgtagaa
     4261 ggtgacgagc ttgaacagtt cgtcgcccag ctcgacgtcc tgatatttgg gcgagttccc
     4321 gttcggcgcc tcgaagcacg ccttctgctt ctcggtgcag tcgcccgcgc cttgcgggct
     4381 catgacggtg gagagcccga tatcgccccc tgcagcctcg gccgtttgtt gcgcgatgct
     4441 gggcaccccg gccttccagc cgaagcggcc gagcatcgcc ttctcatcct cgcgcgacca
     4501 gacgcggttg ggcttgccgg aaatgccgtc catgtccgcg tcatcgggat cggcattggc
     4561 gaggatctgc tcctcgggca c
//
LOCUS       DASUQP010000236        12608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1887303, whole genome shotgun sequence.
ACCESSION   DASUQP010000236 DASUQP010000000
VERSION     DASUQP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12608
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887303"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1146
                     /locus_tag="VFY74_10885"
     CDS             193..1146
                     /locus_tag="VFY74_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932235.1"
                     /translation="MSRLSDQVEAKIARANRAFRLSSRVIVAAHRELESHGEWLDRHS
                     ATWAEEVKRHRRLLNRKLAMRALVRFVVGLVFAAPFALARSLEARLKDKPAPASPQPC
                     LAEDAPAFSPQQTLQHRIRGLEGLPGTKTDAASRLAEPRIESVQRPSSPRDGWPSRVL
                     AAFGNMEKRTATVFALGSVALLLVVHGALHGTPSTARAELTVATKQNAPIDAARASVT
                     VPKTSAKAKRPAQASGFAVLETAPSPQALRAPPQTIADMIGMAQPVSLPPVEAETVPA
                     PVAAAPIPSKPAAKAKPKRKLAVREPEPLPWWQEWSWIRVR"
     gene            1128..1505
                     /locus_tag="VFY74_10890"
     CDS             1128..1505
                     /locus_tag="VFY74_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932236.1"
                     /translation="MDKSALTRRAEGRDGMRLIGEVIAALTIGAMVLALVGFGPSSPD
                     QVLGLNSAYQALAPKSPHRTLGELRCEGRGAIVINVDGRDYAVNGLAGSRYPPIQNVW
                     TENSHPEVDIDRLIVQGITLCDW"
     gene            complement(1701..3227)
                     /locus_tag="VFY74_10895"
     CDS             complement(1701..3227)
                     /locus_tag="VFY74_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5932237.1"
                     /translation="MQVAQLKEPVETSDVTASGIAASSIAADSVAAEASQGGALLKLL
                     TCGSVDDGKSTLIGRLLWDAAGVTEDQRAAVTLASHNRGVNGERIDFSLLLDGLQAER
                     EQGITIDIAWRYFETPERRFIIIDSPGHEQYTRNMATGASHADIAILLVDARHGAKRQ
                     TRRHAAICDLVGIKRVVLAVNKMDEVGWSEARFREIEAEFNAMAGSFNFAETTTIPVA
                     ALTGDNVAYRSTTMPWYLGPTLLDYLESVDPETARLDVPFRMPVQLVLRAAGDFRGYS
                     GTVTSGRIGVGERIVDARSGLGATLRRIATMDGDRSFAAKGDAVTLVLDTDLDISRGA
                     VLSEVPRRPINADVIEARLVWLSEAPFDPESGYLLRTATDLTPVSSVSVSALIDFETL
                     ASQPAHGCGVNDIADCRILLGRSASIDLFRDLPLTGSFVLVSAIDGATVAGGVITWAQ
                     AGAPALGDNVFVIDRALLSRGLCADLGDSPHDQAEFSRRAQELAALMRSAGVPVLIEI
                     "
     gene            complement(3241..4146)
                     /gene="cysD"
                     /locus_tag="VFY74_10900"
     CDS             complement(3241..4146)
                     /gene="cysD"
                     /locus_tag="VFY74_10900"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX5932238.1"
                     /translation="MTALTSHLKLLEAESIHIFREAAAQFRAPVLLYSIGKDSTVLLH
                     LARKAFWPAKPPFPLLHIDTTWKFHDMIAFRDRTARAFGLDLIVHTNHDGLARNMNPF
                     DYPPSVYTDVMKTVALRQALDTGGFDAAFGGARRDEEGSRAKERVFSFRAAGHRWEPR
                     RQRPEMWQLLNGRLGKGETVRVFPLSNWTEGDVWRYIALEKLDVVPLYFAAPRPVVKR
                     NDQLIVVDDARMRFEPGEKPEMRKVRFRTLGCWPLTAAIESDAADLDGIVAETLAAKY
                     SERAGRLIDHDQAGAMEMKKREGYF"
     gene            complement(4143..4892)
                     /locus_tag="VFY74_10905"
     CDS             complement(4143..4892)
                     /locus_tag="VFY74_10905"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX5932239.1"
                     /translation="MPLRRDAARQEADALDQRLIRVKSLTDRIREMRRRVEGRITFST
                     SLGLEDQAILHAIAESGAEIDVFTLDTGRLFPEVLETVELSELRYGVRIRLVTPDAHE
                     VETLVARDGVLGFRHSVENRKACCEIRKVRPLNRELSGAQGWISGLRREHSDERANVP
                     LAQWDEDRALIKVNPVADWTTADLTAYIAANNIPVNPLHARGFVSIGCAPCTRAVQPG
                     EHPRAGRWWWEQEEKKECGLHLNPNRKGRAA"
     gene            complement(4896..5258)
                     /locus_tag="VFY74_10910"
                     /pseudo
     CDS             complement(4896..5258)
                     /locus_tag="VFY74_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
     gene            complement(5245..6900)
                     /locus_tag="VFY74_10915"
     CDS             complement(5245..6900)
                     /locus_tag="VFY74_10915"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX5932240.1"
                     /translation="MYRYDEFDHAFVRERVAEFKDQVERRLAGQLTEDEFRPLRLRNG
                     LYLQLHAYMLRIAIPYGVLSPRQLRQLAMIGRRWDRGYGHFTTRQNLQFNWVKLVDIP
                     DVLAALADVEMHCIQTSGNCVRNVTADPFAGAANDELEDPRPWCEILRQWSTLHPEFS
                     WLPRKFKIAISGAAEDRAAIGFHDIGLKIVRGSNGENGFRVLVGGGMGRTPYVGQEIR
                     AYLPKQHLLSYIESILRVYNLYGRRDNIHKARIKILVNQLGIDKFREEVETDWEATHR
                     DAIDLPEDERKRIMSYFAPLDLAARAVRNEGFDKRRAADPAFANWVKHNVRPHRVPGY
                     STVVVSLKEPGRTPGDASSDAMELVADLAERFSQSEARVNYTQNLMLPHVATAHIVAL
                     YDILAAHGLATGNYDLLGDMICCPGLDYCNLANARSIPIAKEIAKRFEDPKRQELIGP
                     LRLNMSGCINACGHHHSGNIGILGVDKKGTELYQISLGGNPKDDAAIGTIIGPGFRAE
                     AVPQAIDTIIETYLARRSEGETFLDTWRRVGAQPFKEALYGAG"
     gene            complement(6887..7180)
                     /locus_tag="VFY74_10920"
     CDS             complement(6887..7180)
                     /locus_tag="VFY74_10920"
                     /inference="COORDINATES: protein motif:HMM:NF022464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEX5932241.1"
                     /translation="MPNVVTANRLVDGIVVYLSADGGWTEEIGRARLAETEEDTKALE
                     AEAARDVAARKVVAVYPMEVVLHDGIVDPLSVRERIRAAHRTTLTKDWYDVPL"
     gene            complement(7173..8603)
                     /gene="cysG"
                     /locus_tag="VFY74_10925"
     CDS             complement(7173..8603)
                     /gene="cysG"
                     /locus_tag="VFY74_10925"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEX5932242.1"
                     /translation="MRTFPILVSFDGKPPLVTGGGELAAVKTRLLLKRAPSVDVAATE
                     VIPELAALIEEGTVSLIAAKPGIDQLRGRPLVIAATEDDEEDARVSAIARALGVPVNV
                     PDRPELCTFALPAIVDRGEVTVAIGTSGASPVLAQRLRAWLEQELHPRLDALAKLAAE
                     FRGAVAEALPSGQPRRKFWEAVFEGAAAEAMLEGDENKARNLIEAAIAKAAVAPRAGG
                     RVLLVGAGPGDPELLTMKAVRALKAADVIFYDRLVGEDVLDHARREAELIPVGKAKGA
                     HSVPQEEINALLIERALGGQTVVRLKGGDPFIFGRGGEELDALREAGVEIEIVPGITA
                     GIAAAASLQIPLTHRDVSHTVTFVSGHEAGGAEPSFEHLDLAALASGNNTLLVYMGVT
                     TAGIIAKSLIEAGFRPGLPVIAVENASRDNERRVRATIAELAAGPERLGLNSPAVLIF
                     GEVAGLPASGMVDTILSLEEVKRLYA"
     gene            complement(8610..8960)
                     /locus_tag="VFY74_10930"
     CDS             complement(8610..8960)
                     /locus_tag="VFY74_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5932243.1"
                     /translation="MFLNLRMPDGTTHRLEALEGWRIMEVIRDWGLPIKAECGGACAC
                     ATCHVWVGADWVAKLTPPSSEELEMLDGAFSVDERSRLCCQIIMSKDLDGLVVELAPE
                     SLGDAPAERVAVGG"
     gene            complement(9745..10203)
                     /gene="mscL"
                     /locus_tag="VFY74_10935"
     CDS             complement(9745..10203)
                     /gene="mscL"
                     /locus_tag="VFY74_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5932244.1"
                     /translation="MLKEFKEFALRGNVVDMAVGIVIGAAFSTIVKSLVDDILMPPIG
                     VVTGGVDFSNMFVALNGEHYDSLAQARQAGAPTVNFGVFVNNVIAFLIVAMALFLVIK
                     AMNHLRRRQEEAPATEPPPSQEVQLLREIRDALVTGGVTPARPPRPRRGT"
     gene            10341..10628
                     /locus_tag="VFY74_10940"
     CDS             10341..10628
                     /locus_tag="VFY74_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/substrate symporter small subunit"
                     /protein_id="HEX5932245.1"
                     /translation="MAAERPESGDPARARHNIQTLILAVGILSIVLLAVIFAVTSLSW
                     FNRFGFLHFPLGFYLLAQGLLILIVVMAFWFIRIQERIDLSRAESEEWVGR"
     gene            10625..12508
                     /locus_tag="VFY74_10945"
     CDS             10625..12508
                     /locus_tag="VFY74_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932246.1"
                     /translation="MTVPSRLRSPNPHLGAYYGIVTATLVSLVIMLAMFEQLGWARTG
                     LAQTMMIVPLALYLLIAVGARTREAQDFFVSGRRVPPVYNGFVLAAIAVGGAGFLGYT
                     GTAFFLGFDAMAIGLGWTFGMLAAAILFVPYLRKAGSYTLPSFFGHRFRSRSLRATAS
                     ALQLPPLALLLAAEIKIAALIASLFLPLSFSVAVIMVAALVAAIGILGGMRSITWVGS
                     AEFLVGAVGLAAVVTIVSILLTNLPAPQLTYGELLADLQQAEATTGVAPLSPGVPSAA
                     LPGAALRPAVKPFLQPSGALGPVPFLTLFLSLALGTAALPTLLARSGVTATVADQRRS
                     TAWGLLLVAAFAMTVPAMAVFAKLTIFREIAAAKAASLPGWLTALGDMRLLLTGDANG
                     DGTIAAAELLIGRDGIALALPAAAGLPYVLTALMATAALAVALAASASHLFTLAASLS
                     EDLYRTFDRRQALPRLVAAWAAIAATALAAAVFLVIADVDPLKSALMAFAFAAATFFP
                     ALLLAIWWPRCTRWGATTALAAGFAVMVTDALFGKALGLFGAGFSAPLASLAGVVLAL
                     ITGIAASYAQAAPSKAEADYREAMRNPDGDAVYDRAQARAAAAAASAAARAEQVAATE
                     SSR"
BASE COUNT         2058 a         4143 c         4125 g         2282 t
ORIGIN      
        1 aaagcggaac gcggcccttt agcttcagtc tttcttaaaa aacacttcag tctttcttaa
       61 gcgcttggcg gtctgattct cggcacgggt gcgcacaggt tgcgcgtcag gcaccggcgt
      121 ccggctttga ggccgttcgc cttggtccct gaaactttgc gcgcgcccgc ttgaagagtg
      181 ggagccaagg ttgtgtcgcg tttgtcggac caggtcgagg cgaagatcgc ccgcgccaac
      241 agggcgttca ggctgtcgag ccgcgtcatc gtcgccgctc accgggaatt ggagagccac
      301 ggcgagtggt tggaccgcca cagcgcgacc tgggccgagg aggtgaaacg gcaccgccgc
      361 cttctcaacc gcaaattggc gatgagggcg cttgtgcggt ttgttgtcgg cctcgtcttc
      421 gcggcgcctt tcgctctggc gcgatccctt gaggcgaggc tgaaagacaa gccggcaccc
      481 gcatcgccgc agccgtgtct tgccgaagac gcgcccgcct tttcgccgca acagacgctc
      541 cagcatcgca ttcgcggact tgaggggttg cctggcacga agaccgacgc ggcctcgcgc
      601 ctcgccgagc cacggatcga aagcgttcag cgaccctcgt cgcctcgcga cggctggccg
      661 tcacgcgtcc tcgccgcgtt cggcaatatg gaaaagcgaa cggcgactgt ttttgcgctc
      721 ggttccgtgg cgcttcttct cgtcgttcac ggcgctcttc acggcacgcc ctccacagcg
      781 cgtgccgaat taaccgtagc tacgaaacaa aatgcgccca tcgatgccgc ccgagcgtcg
      841 gtgacggtgc cgaagacatc cgcaaaggcc aagaggccgg ctcaagcttc aggcttcgcc
      901 gtcctcgaaa cggcgccgtc accccaggcg ctgcgagcac cgccccagac catcgccgac
      961 atgatcggga tggcgcagcc cgtttctttg ccgcccgtgg aagcagagac tgtgcctgcg
     1021 cccgttgccg cggcgccgat cccgagcaaa cccgcggcga aggcgaaacc caaacggaag
     1081 ttggccgtgc gagaaccgga gcctcttccc tggtggcagg aatggtcatg gataagagtg
     1141 cgctgacgcg tcgcgcggag ggccgggacg gcatgaggct catcggcgaa gtcatcgcgg
     1201 cacttactat cggcgcgatg gtgcttgctc tcgtcggctt cgggccgagc tcgcccgatc
     1261 aggtcctcgg tctgaattcg gcctatcagg cgttggcgcc gaaatcgccg catcggacgc
     1321 tgggcgaact tcgctgcgag gggcgcggtg ccatcgtcat caatgtcgac ggcagggatt
     1381 atgccgtgaa cgggttggca ggctcgcgct atccgccgat ccaaaatgtc tggaccgaga
     1441 actctcatcc cgaggtcgac atcgatcgcc tgatcgtcca gggcatcacg ctttgtgatt
     1501 ggtgagttgg gggcctggcg cgaacccggg ccgtgccctt cctcttatcc tttgttgaac
     1561 atcacccagg ccgcgtaaca cagcatcgcc gcgaggatcg gcctgagcag ccattccggc
     1621 gcaaggcctg cgagccgcgc gccgagcaag atgcctggga ttgagcctcg cccccttgcc
     1681 ccggcgccga gagtgacggg ctagatctcg atcaggaccg gaactccggc tgaacgcatg
     1741 agggccgcga gttcttgcgc ccggcggctg aactcggctt ggtcgtgcgg cgaatcgcca
     1801 agatcggcac agagcccgcg ggaaagaagc gcccggtcga tgacgaacac attgtcgccg
     1861 agcgcgggcg ctccagcctg agcccaggtg atgacgccgc ccgccacggt cgcgccatcg
     1921 atggcgctga ccagcacgaa gctcccggtg agtggcaggt cgcggaagag atcgatcgat
     1981 gccgaccggc cgagcaggat gcggcaatcg gcgatgtcgt tgaccccgca cccatgggcg
     2041 ggttgtgagg caagcgtttc gaaatcgatc agagcggaga cgctcacgct ggagaccggg
     2101 gtcaagtcgg tcgcggtccg caagagatat cccgattccg gatcgaacgg cgcctccgac
     2161 agccacacga gccgcgcttc gatcacatcg gcattgatcg ggcggcgcgg aacttcggac
     2221 agaaccgcgc cgcgggaaat gtcgaggtcg gtatcgagca cgagcgtcac ggcatcgccc
     2281 ttggcggcaa aggaccggtc gccatccatc gttgcgatgc gacggagcgt ggcgccgagg
     2341 cctgatcgcg catcgacgat gcgctcgccg acgccgatcc ggcccgaggt gaccgtgccg
     2401 gaatagccgc ggaagtcgcc ggcggcgcgg agcacgagtt gcaccggcat gcggaacggc
     2461 acatcgagac gcgcggtctc gggatcgacg gactccagat agtcgagcag cgtcggcccc
     2521 aaataccagg gcatcgtcgt cgagcggtag gcgacgttgt cgccggtcaa ggcagcaacg
     2581 ggaatggtgg tcgtctcggc gaagttgaag ctcccggcca tggcgttgaa ctcggcctcg
     2641 atctcccgga agcgcgcctc cgaccagccg acttcgtcca tcttgttgac cgcgagcacc
     2701 acgcgcttga tgccgacgag atcgcagatc gccgcgtgac ggcgggtctg gcgcttggcg
     2761 ccgtgacggg catcgacgag caggatggcg atgtcggcgt gggaggcgcc ggtcgccatg
     2821 ttgcgcgtgt actgctcgtg cccgggcgag tcgatgatga tgaagcggcg ctcgggcgtc
     2881 tcgaaatagc gccaggcgat gtcgatggtg attccctgct cgcgctcggc ctggagcccg
     2941 tcgagcagga ggctgaaatc gatgcgctcg ccgttgaccc cgcggttatg cgaggcaagg
     3001 gtcaccgccg cgcgctgatc ctcggtgacg ccggccgcgt cccacaacag ccggccgatc
     3061 agggtcgatt tgccgtcgtc gaccgagccg caggtgagca gcttgagcag agcgccgccc
     3121 tgcgaggctt cagccgccac gctatccgcg gcgatgctgc tcgcggcaat gccgctcgcg
     3181 gtaacgtcgc tcgtctcgac cggctctttc agttgcgcca cttgcatcga ccaagcccct
     3241 ttagaaatac ccttcgcgct tcttcatctc catggcgccg gcctgatcgt ggtcgatcag
     3301 ccgcccggcg cgctcggaat atttggccgc gagcgtttcc gccacgatcc cgtcgagatc
     3361 ggcggcgtcc gattcgatcg ctgcggtgag cggccagcat ccgagcgtcc ggaagcgtac
     3421 tttgcgcatc tccggcttct cgcccggctc gaagcgcatg cgcgcgtcgt caaccacgat
     3481 cagctgatcg tttcgcttaa ccaccggacg gggcgccgcg aaatagagcg gcaccacgtc
     3541 gagcttttcc agcgcgatgt agcgccacac gtcgccctcg gtccaattcg acagcggaaa
     3601 gacgcgcacc gtctcgccct tgccgagacg cccgttgagg agttgccaca tctcgggacg
     3661 ctggcggcgc ggctcccagc gatggcccgc ggcgcggaag gagaacacgc gctccttggc
     3721 gcgcgatccc tcctcgtcgc ggcgcgcgcc gccgaaagcg gcatcgaagc cgccggtatc
     3781 gagcgcctgg cgcagcgcca ccgtcttcat cacgtcggtg tagacggacg gcggatagtc
     3841 gaagggattc atgttgcggg cgagcccgtc gtggttggtg tggacgatga ggtcgagccc
     3901 gaacgcgcgc gccgtacggt cccggaaggc gatcatgtcg tggaacttcc aggtcgtgtc
     3961 gatatggagg agcgggaacg gcggctttgc cggccagaag gctttccgcg cgaggtgcag
     4021 gaggacggtc gagtccttgc cgatcgaata gagcagcacg ggcgcgcgga actgggccgc
     4081 cgcctcgcgg aagatatgga tgctctccgc ctcgagcagc ttgagatgcg aggtgagcgc
     4141 ggtcatgcgg ccctcccttt ccggttcgga ttgagatgga gcccgcattc cttcttctcc
     4201 tcttgctccc accaccagcg gccggcccgc ggatgctcgc cgggctggac cgcgcgcgtg
     4261 cagggcgcgc agccgatcga gacgaagccg cgcgcgtgca gcggattgac cggaatgttg
     4321 ttggcggcga tgtaggcggt gaggtcggcc gtcgtccaat cggcgacagg gttgaccttg
     4381 atcagcgcgc ggtcctcgtc ccattgggcc aaaggcacgt tagcgcgctc gtcggaatgc
     4441 tcgcgccgga gcccgctgat ccagccttgc gcgccggaaa gctcgcgatt gagcggcctc
     4501 actttgcgaa tctcgcagca ggctttgcgg ttctcgaccg aatggcggaa gccgagcacc
     4561 ccgtcgcgag cgacgagcgt ctcgacttcg tgagcgtctg gcgtcaccag gcgaatgcga
     4621 acgccatagc gcagctccga gagttccacg gtctcgagca cttcgggaaa caggcggccg
     4681 gtatcgaggg tgaacacatc gatctctgcg ccggactcgg cgatggcgtg caggatcgcc
     4741 tgatcctcga gcccgaggct ggtcgagaag gtaatccggc cctcgacccg ccgccgcatc
     4801 tccctgatgc gatcggtcag gctcttaacc cggatgaggc gctgatcgag cgcgtcggcc
     4861 tcttggcgcg cggcgtcgcg gcggagcggc aaggggttgc ggaagccccg ctgatagttg
     4921 tgcttgatcc gcgacacgga caccttccac accggcgcct gctcgggatc ggcgatctcg
     4981 gcggtgtcga agcccgagcg cagcaggaat tgcgcgaggt cgggaatgag gtgcccgacg
     5041 gctctgatct cgcccttgaa gccgtggcgc tcgcggagca aaacggcgag gctgatgccg
     5101 cgaccgtcgt tgaacacggg aaaccggatg gcgatcggcc cggcattgcc gccggcagcg
     5161 gcagcgagcg cggctgcctc ggcgttcggc tcgagcagcg tcgcaggccc cgccggatcg
     5221 ggcaagagcg aaccggtggc gagatcaacc agcgccatag agagcctcct tgaagggctg
     5281 ggcgcccacg cgccgccacg tgtcgaggaa ggtctccccc tcgctccgcc tggcgagata
     5341 cgtctcgatg atggtgtcga tggcttgcgg caccgcttcg gcgcggaagc ccgggccgat
     5401 gatggtgccg atcgcggcgt cgtccttcgg attgccgccg agcgagattt ggtagagctc
     5461 ggtgcctttc ttgtcgacgc cgaggatgcc gatattgccg gaatggtgat ggccgcaggc
     5521 attgatgcag cccgacatgt tgagccggag cgggccgatc agctcctggc gcttcggatc
     5581 ctcgaagcgc ttggcgatct ccttggcgat cgggatcgag cgggcattgg ccagattgca
     5641 gtaatcgagg cccgggcagc agatcatgtc gcccaagagg tcgtaattgc cggtggcgag
     5701 cccgtgcgcg gcgaggatgt cgtagagcgc gacgatatgg gcggtcgcga catggggcag
     5761 catcagattc tgcgtgtagt tgacgcgcgc ttccgattgg ctgaagcgct cggcgagatc
     5821 ggcgacgagc tccatggcgt cggagctcgc gtcgcccggc gtgcgccccg gctccttcag
     5881 cgagacgacc acggtggaat agcccggcac gcggtggggc cgcacattgt gcttgaccca
     5941 attggcgaag gcgggatcgg cggcgcggcg cttgtcgaag ccctcgttgc gaacggcccg
     6001 cgcggcgaga tccagcggcg cgaaatagga catgatgcgc ttgcgctcgt cctcggggag
     6061 atcgatcgca tctctgtgcg tcgcctccca atcggtctcg acttcctcgc ggaatttgtc
     6121 gatgccgagc tgattgacca ggatcttgat gcgcgccttg tggatgttgt cgcgccgccc
     6181 atagagattg tacacgcgca ggatcgactc gatgtaggag agcaggtgct gtttcggcag
     6241 atacgccctg atctcctgcc cgacataggg cgtccgcccc atgccgccgc cgacaagaac
     6301 gcggaagccg ttctcgccgt tcgacccgcg cacgatcttc aagccgatat cgtggaagcc
     6361 gattgccgcg cggtcttctg cggcccctga gatggcgatc ttgaacttgc gcggcagcca
     6421 cgagaactcc ggatgcagcg tcgaccattg gcgcaagatc tcgcaccacg ggcgcgggtc
     6481 ctcgagctcg tcattggcgg cgccggcgaa cggatcggcg gtgacgttgc gcacgcaatt
     6541 gccgctggtc tggatgcagt gcatctcgac atcggcgagc gccgccagca catcgggaat
     6601 atcgaccagc ttcacccagt tgaactgaag gttctggcgc gtggtgaaat ggccgtagcc
     6661 gcggtcccag cgccgcccga tcatggcgag ctggcgcagt tgccgcggcg acagcacgcc
     6721 ataggggatg gcgatgcgga gcatataggc gtggagttgg agatagagcc cgttgcgcag
     6781 gcgcagcggc ctgaactcgt cctcggtcag ctggccggcc agccggcgct cgacctggtc
     6841 cttgaactcc gccacgcgtt cgcgcacgaa ggcgtggtcg aactcgtcat agcggtacat
     6901 cgtaccaatc cttggtgagc gtcgtgcggt gggcggcgcg gatgcgttcg cgcacggaaa
     6961 gcggatcgac gatcccgtca tggagcacga cctccatggg atagaccgca acgactttgc
     7021 gcgcggccac atcgcgagcg gcctcggctt cgagcgcctt ggtgtcttct tccgtctcgg
     7081 ccagacgcgc gcggccgatc tcctcggtcc agccgccgtc ggctgagagg tagacgacga
     7141 tgccgtcgac gagacggttt gctgtgacga cgttaggcat agaggcgctt gacctcctcc
     7201 aggctcaaga tggtgtcgac catgccggag gcgggaagtc ccgccacctc gccgaagatc
     7261 agaacagccg ggctgttgag gccgaggcgc tccgggcctg cggcaagctc ggcaatggtc
     7321 gcgcgcacgc ggcgctcgtt gtcgcggctc gcattctcga cggcgatcac gggcaagccc
     7381 ggcctgaagc ccgcctcgat caggcttttg gcgataatcc ccgccgtggt cacgcccata
     7441 tagacgagca gcgtgttgtt gccggacgcg agcgctgcaa gatcgagatg ctcgaacgac
     7501 ggctcggcgc cgcccgcctc gtgacccgac acgaaggtca cggtatgcga cacgtcgcga
     7561 tgggtgagcg ggatctgcaa gcttgccgcg gccgcgatgc cggcggtgat gcccggcacg
     7621 atctcgattt cgacgcccgc ttcgcgcagc gcatcaagct cctcgccgcc tctgccgaag
     7681 atgaacgggt ccccgccctt cagccggacc acggtctgac cgccaagcgc ccgctcgatg
     7741 agaagcgcgt tgatctcctc ttgcggaacg gagtgcgcgc ccttggcctt gccgacaggg
     7801 atcagctcgg cttcgcggcg cgcatgatcg agcacgtctt cgccgaccag gcggtcgtag
     7861 aagatcacgt ctgcggcttt aagcgcgcgc accgccttca tggtgagaag ctcgggatcg
     7921 ccgggacccg cgccgaccag aagcacgcgg cccccggccc gaggcgccac agcagccttg
     7981 gcgatcgcgg cctcgatcaa attgcgggcc ttgttctcgt cgccttcgag catggcttcg
     8041 gcggccgcgc cctcgaacac cgcctcccag aatttccgcc gcggctggcc cgagggcaga
     8101 gcttcggcta cggcaccgcg aaactctgcc gcgagtttgg cgagcgcatc gagccgggga
     8161 tggagttcct gttcgagcca ggcgcgcaga cgctgcgcca ggaccgggga cgcgcccgat
     8221 gtgccgatcg cgaccgtcac ctcgccacga tcgacgatcg cgggaagcgc gaaggtgcaa
     8281 agctcgggcc ggtcgggaac gttcaccggc acgccgagcg cgcgcgcaat ggcagacacc
     8341 cgggcgtcct cttcgtcgtc ctcggtcgcg gcgatgacga gcgggcgtcc gcgcaactga
     8401 tcgatgccgg gcttcgccgc gatcagcgac accgtccctt cctcgatcag cgcggcaagt
     8461 tcgggtatga cctcggtggc ggcgacatcg acggaaggcg cgcgcttgag caggagccgc
     8521 gtcttgaccg ccgcaagctc gccccctccg gtcacgagcg gaggcttgcc gtcgaagctc
     8581 acgagaatag ggaaggtccg catcgccgct tatcctccca ccgccacgcg ctcggccggc
     8641 gcatcgccaa ggctctccgg tgcaagctcg actacgagcc cgtcgaggtc cttcgacatg
     8701 atgatttggc agcacaggcg cgagcgctca tcgacgctga aggcgccgtc gagcatctcg
     8761 agttcttcgc tgctcggcgg ggtgagcttc gccacccagt ccgcgccgac ccagacatgg
     8821 catgtggcgc aggcgcaagc gccgccgcac tcggccttga tgggcaggcc ccagtcgcgg
     8881 atcacttcca tgatgcgcca cccctcgagc gcctcgagac ggtgggtcgt cccgtcgggc
     8941 atgcgtaagt taaggaacat gtcaggcggc ctcgagcaga cgttgagaac ccgcagtttc
     9001 taggaatgta gcgaaaaacc attctcctaa aaaggcttca gcggcaaaaa gacgcaaaat
     9061 ggtttcttcg acgtgtcgaa agtagtaatt ttttctgcaa gaggcgattg gcgcaacgtc
     9121 ccgcccctac tcaacccttg ccgcgcaaaa tccttgctgc gcttcgggct ccgcgctggc
     9181 cgcccgctac cagggtatca cgggatctct tgcagggctg caattcttgc gagggcgcca
     9241 gcgaatcgta atgctcgcca tttgagcgca acgaacctga caatgtcgct aaatgcgaga
     9301 catctcgtag cggcattctt aacccgaagt tcctcatttc tttggttgag cagaagagtt
     9361 cccgtacctt tgggagggtc gactagatga aggggcgttc gaaggagagg cacccaatgg
     9421 acccagtacc ggctccccat ctgcaattta tcggagtttt agcgcaagct gccctgcatc
     9481 tcgggggcag acggctttcg ccgaagcagc gggagctatt gttgcgtcta gcagcagccc
     9541 cgagcccacg ccgctaaagt tttccggcac cgacgcagcc gctttaggca ggcgcgaaag
     9601 cgcgactgcg cacgttctgc tgcgcctcag cgctgacttc gcttgggtgc gatcgacact
     9661 gctctcagat cgattgcagt ggccggtcga ctgcacagcg ttctgccagt cgcaagcctt
     9721 gccgcgcaaa atctttgttg cgcgtcaggt gccgcgccgc ggtctcggcg ggcgcgcggg
     9781 agttacgccg cctgtaacca atgcatcgcg aatctctcgc aggagctgca cttcctgcga
     9841 gggcggcggt tcggtcgccg gcgcttcctc ctgccttcgg cgcaaatggt tcatggcctt
     9901 gatcaccagg aacagcgcca tcgccacgat caagaaggcg atgacgttat tgacaaacac
     9961 gccaaaattg acggtcggcg ccccggcctg tctggcttgc gccagcgagt cgtaatgctc
    10021 gccgttcagc gcgacgaaca tattggagaa atcgaccccc ccggtcacca cgccaatggg
    10081 cggcatgagg atgtcatcga ccagggactt cacgatggtt gagaacgcgg cgccgataac
    10141 aatgccgacc gccatatcga ccacattccc cctaagggcg aattccttga actccttcag
    10201 cattcgctga tctccctctc tgggctcaac agccttagtc accaccctgc cacgattaaa
    10261 cggcattggt accgcgctgg tttgccagcc gggcacgcgc ttggtaacgc gttggccggc
    10321 cttgaaccag ggggagtgaa atggcggcag aacgtcccga gagcggcgat ccggcgcggg
    10381 cccgtcataa cattcagacc ctgatcctcg ccgtcgggat tctgtccatc gtgctgcttg
    10441 ccgtcatttt cgccgtcacc tctctctctt ggttcaatcg tttcggcttc ctgcatttcc
    10501 ctctcggctt ctatctgctg gcgcaagggc ttctcatcct tatcgtggtg atggcgttct
    10561 ggttcatccg catccaagaa cgcatcgacc tttcgcgcgc cgaaagcgag gaatgggtgg
    10621 gacgatgacg gtcccctcgc ggctccgcag ccccaacccg catctcggcg cctattacgg
    10681 catcgtcacc gcgacactcg tatcgctcgt catcatgctc gccatgttcg agcaactcgg
    10741 ctgggcgcgc acgggcctcg cccagaccat gatgattgtg ccgctggcgc tctatctcct
    10801 catcgccgtg ggcgcccgca cgcgtgaggc ccaggacttc ttcgtttcgg gacggcgggt
    10861 gcctcccgtt tataacggct tcgtgctggc cgcgatcgcc gtcggcggcg cgggcttcct
    10921 cggttatacc ggcactgcct tcttcctcgg tttcgatgcc atggcgatcg gcctcggctg
    10981 gaccttcggc atgctcgcgg ccgcgatctt gttcgtgccg tatttgcgaa aagcgggctc
    11041 ctataccctg ccgagcttct tcggccatcg cttccgctcg cggagcttgc gcgccacggc
    11101 gagcgctttg cagctcccac cattggcgct gctcctcgcc gccgagatca agatcgcggc
    11161 cctgatcgct tcgctcttcc tgccgctttc cttctcggtc gccgtaatca tggtggcggc
    11221 gctggtcgcg gcgatcggca tcctcggcgg catgcgctcc atcacctggg tgggaagcgc
    11281 tgagttcctc gtcggcgcgg tggggcttgc tgcggttgtg accatcgttt cgatccttct
    11341 taccaacctg cccgcgccgc agctgaccta tggcgagctt ctcgcggact tgcagcaagc
    11401 cgaggcgacg acaggcgtcg cgccgctttc gcccggcgtc ccttcggctg cgttgcccgg
    11461 agcggcgcta aggcccgcgg tcaaaccgtt tctgcagccg tccggcgcgc ttgggccggt
    11521 cccgttcctc acgctgttcc tgtcgcttgc tctcggaacg gccgcccttc cgacccttct
    11581 tgcgcgaagc ggcgtcacgg caaccgtcgc cgatcagcgc cgctcgacgg cctggggact
    11641 gctgttggtt gccgctttcg ccatgaccgt tcccgccatg gcggtcttcg ccaagctcac
    11701 gattttccgc gagatcgcgg ccgccaaggc cgcgtccctt cccggttggc tcaccgcgct
    11761 tggcgacatg cggctccttc tgacgggaga tgccaacggc gacgggacga tcgctgcggc
    11821 cgagcttctg atcgggcgcg acgggattgc gctcgcgctg cccgcggcgg cgggcctccc
    11881 ttacgtgctg acggctttga tggcgacggc ggcgttggcg gtcgcgctgg ccgcctcggc
    11941 gtcgcatctc tttaccctcg ccgcgagcct ctccgaggat ctttaccgta ccttcgatcg
    12001 ccgccaggcg ctgccgcggc tcgtcgccgc ctgggccgcc attgccgcca ctgcgctcgc
    12061 cgccgccgtc tttctcgtta ttgccgatgt cgatcccctg aagtcggcgc tgatggcctt
    12121 cgcatttgcg gcggcgacat ttttcccggc gctgcttctt gccatctggt ggccgcgctg
    12181 cacgagatgg ggtgcgacga cggcgctggc ggctggcttt gcggtgatgg tgaccgatgc
    12241 gctgttcggc aaagcgctcg gccttttcgg cgcaggcttt agcgccccgc ttgcgagcct
    12301 cgccggcgtc gttcttgcgc tcatcaccgg cattgccgca agttacgccc aagccgcccc
    12361 gtcgaaggcc gaagccgact atcgcgaggc gatgcgcaat cccgacggcg atgccgttta
    12421 cgatcgcgct caggcccgcg cggcagcggc ggcggcctcg gcggcggcga gggccgagca
    12481 ggtcgcggca acggaaagct ccagatgaac gacgcaccgt cccccaaaga gaagcggctc
    12541 acgctctatc ccgagctcgg tccccaccgc accgggcggc tcagagtctc ggagctgcat
    12601 gaactcta
//
LOCUS       DASUQP010000237        13067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_662842,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000237 DASUQP010000000
VERSION     DASUQP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13067
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662842"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFY74_10950"
     CDS             complement(<1..385)
                     /locus_tag="VFY74_10950"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5932247.1"
                     /translation="MSRSAPTLAAGLLCLGLVGCALAGGGRPPATYDLVAPRSFAAAP
                     RAAPWQLVVYEPTAVHALETNRLMVRPKADQVSYYKGVAWSDRLPRLVQARIIETFQN
                     SGAVKAVSSSTGQYALATELRAFQID"
     gene            complement(438..1274)
                     /locus_tag="VFY74_10955"
     CDS             complement(438..1274)
                     /locus_tag="VFY74_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5932248.1"
                     /translation="METKANYLMIGGFVLGVLALAFIFVFWMSNFAGGGKRYSIVFES
                     SVAGLTTGSSVGFNGIRVGEVQSFALDPEDARKVQVLVSVRDDTPVRENSHASIQSMG
                     LTGGSGIQITPGTPDSPILVATAENPIPVIRADPGSGQGVFEAGSAALNNANVFISKL
                     NALLDQNEKSINTTMTNVEQFTTMLNAKSDDIGLTISNVKEGAESFKSLSTKLEVSLG
                     DNMEGLSRQARESLQEFGNFMREGRRAAGTLNRILEKLEADPRGFLLGGSQVPEYTPR
                     GR"
     gene            complement(1306..2106)
                     /locus_tag="VFY74_10960"
     CDS             complement(1306..2106)
                     /locus_tag="VFY74_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5932249.1"
                     /translation="MIVENGREVVIRVRGLAKSFGSHRIWDGLNLDVYRGEILAIVGG
                     SGQGKSVLMRVITGLVKPDVGDVGLFGQNTKTLSPQDRLDIERRWGILFQDGALFSSL
                     TVLQNIQVPMREHLELPQAMMDDLAFLKLSMVGLPPNAAYKFPSELSGGMRKRAGLAR
                     ALALDPEIVFLDEPTAGLDPIGATDFDHLIRKLQQTLGLTVYMVTHDLDTIFTVCDRV
                     AVLADKKIVRTGSPAELQRQPNHPWIQEYFCGERARAASPGERPRIPA"
     gene            complement(2103..3281)
                     /locus_tag="VFY74_10965"
     CDS             complement(2103..3281)
                     /locus_tag="VFY74_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5932250.1"
                     /translation="MLKRDHQVVLPTQARAPGFRVELRGTTFRLVATGAWTVAEAAQL
                     DMALKRLRVPIPPTADFTGEMDIAGIAEIDTAGAMLLQRTAAAWERSGLRTRYAGATE
                     GFRILIDEVRRRGGAERPTIEKRKPLQFFTDDMTRALTGVWRDAVQLTAFLGEITSAL
                     GRMIRQPWRFRPTSFIHHLDHVGFRAVPIIALICFLIGAVVMQQGVVQLKPFGAEPFA
                     VNMVAILALREVGILLTAIMVAGRSGSAFTAEIGSMKMREEIDAMRTLGIDPMDTLVL
                     PRILALVLALPLLTFIGDLMGLAGGGVMAFAVLGLDASNYIDKLRDVVDFKHFMAGMI
                     KAPFAAVIIGLVGCLEGLKVEGSAESLGTHVTSAVVKAIFLVIILDAVFAMFLSGIGV
                     "
     gene            3366..4394
                     /gene="dgcA"
                     /locus_tag="VFY74_10970"
     CDS             3366..4394
                     /gene="dgcA"
                     /locus_tag="VFY74_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HEX5932251.1"
                     /translation="MAGLGRGLDGRKSRGSGLALSVATERWPIRGGFAISRGIKREAV
                     VVVATLSDGTHTGRGECVPYGRYGESVEATVAAIRARAPRVANGVSRTELLEALPPGA
                     ARNALDCALWDFEAKSSGRSASELAGLGTLKPVLTAFTLSLASPEAMAEKARAASDYP
                     LLKLKLGGEGDAERLAAVRDAAPSARLIADANEAWRPEELERLLDAAALAGVELIEQP
                     LHASDDEILQHIERPVPICADESVHDRASLEGIAARYDAVNIKLDKTGGLTEALAVTA
                     HARALGLKIMVGSMVATSLAMAPALLLAQDADFVDLDGPLLLLRDRIPGLTFVGGEIL
                     PAPPELWG"
     gene            complement(4411..5631)
                     /locus_tag="VFY74_10975"
     CDS             complement(4411..5631)
                     /locus_tag="VFY74_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5932252.1"
                     /translation="MGKLPPPARAGHPFAWRLGFLYAALFLVVGCYLPFMPVWLDWRS
                     LGPDEIAVLLAAPLFTRIFFTPLIGFAADLAGSRRNIVIALAWGSLLSFLLFWAANGF
                     WQMLLASILLAINWTTITPLVETVATSGIRKGALDYGRVRLWGSLSFIVASLSSGVII
                     GKIGPQVILPLLVAGTALLVLGAHLLPRELGDQGRGPSMLRGLKFADALRLVHAPLFL
                     LFLLATSLIQASHALYYSFGTLHWRAQGIPDGTIGALWSVGVIAEVALFAVSGRVIAH
                     FGTARLLVLAGLATTLRWGLMALDPPLWILGPLQTLHAMSFGAAHLAAIHFLTHAVPE
                     NRAATAQGVYAAVVAGIALGTFTLASGPLYESLAGAAYAAMAAIALAGAGGAYVLAQR
                     WRGEQVVGAMMHGH"
     gene            complement(5695..6885)
                     /locus_tag="VFY74_10980"
     CDS             complement(5695..6885)
                     /locus_tag="VFY74_10980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX5932253.1"
                     /translation="MRVLIATDAWHPQVNGVVRTYERLASEAPALGFQLSFLAPPHFR
                     TFPCPTYPEIRLALAGPRAIARHVEAARPDFIHIATEGPIGFMTRRYCRKTKWPFTTS
                     YHTRFPEYLSARLPVPESWGYALQRRFHNGAAGTFVATPSLASDLEARGFTRLMPWSR
                     GVDTELFKPRNVRLFGPPPVFLYVGRIAVEKNVKAFLDLDLPGRKVVVGDGPQARDLK
                     RHYPNALFTGPRDGVALAEAYASADVFVFPSMTDTFGLVLLEALAAGLPVAAYPTTGP
                     LDVITDARAGVLGPDLRAAALEALALDRAAARAHALRFSWEHSARQFFENVLAAHHLG
                     LPARLSRRKRRVQKKTARLGGTRPSTLMRQFGGRFGGTRVSLEMVSGPAGFKGVVKNF
                     RRIV"
     gene            complement(6885..7712)
                     /locus_tag="VFY74_10985"
     CDS             complement(6885..7712)
                     /locus_tag="VFY74_10985"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX5932254.1"
                     /translation="MKAGTDVDVKHEPRMKRYRAIFISDLHLGTKACQAEAFIDFLRC
                     HDASKIYLIGDIVDFWRIKRGIYWPQSHNDVLQKLLRRARKGTEIIYVPGNHDEAMRD
                     YCGTRFGGVAIERSAIHVAADGRRYLIMHGDEFDVVVRYAKWLALFGDWSYAFALWAN
                     THFNTIRRLLGLPYWSLSAYLKQKVKRAVNYIGEFEAALAHEARRRGAQGVICGHIHH
                     AAMRQVGDIAYVNTGDWVESRTAVAETDDGIFEIIRWGHPEGRPRFELLVDELEAAA"
     gene            7759..8658
                     /locus_tag="VFY74_10990"
     CDS             7759..8658
                     /locus_tag="VFY74_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX5932255.1"
                     /translation="MADKVKGGPMEHNGLVTRLAGALGSTVVDARPLAVGFGLIGLEI
                     RLDDGRHLAVKARASGQGRASDLSLEGYMLGELARHSDFPVPRVHHAEPGLLAMDFIE
                     TDGSGITPSVERHAGELIARLHATPRAAFGYERDTLIGPLPQPNPISRSWVPFFRDQR
                     LLFMAKQAHLEGALPSSMLHRIERLAGRIGDYIIEPAFPSLLHGDLWTGNVLVRGGRI
                     AGFVDPAIYCGHPEIEVAFATLFGTFGEAFFEAYESSMPLEPSFREVRLDLYNLYPLL
                     VHVRLFGSGYLAGIDRTLARVGL"
     gene            9314..10159
                     /locus_tag="VFY74_10995"
     CDS             9314..10159
                     /locus_tag="VFY74_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5932256.1"
                     /translation="MKLLRYGAVGQEKPGVVDADGAIRDLSGVVADLAGAALGRKSLD
                     RIRALDLAALPLVQGAPRIGPPVGVVPKFLGIGLNYSDHAAETGLPLPEVPIVFAKAS
                     SSVSGPNDPVLAPSDFKRMDYEVELGAVIGSPAKNVSEAEALDHVAGYAICNDISERS
                     LQKGGPGEWIKAKSYDTFGPLGPWLVTTDEIPDPQALDLALDLNGARMQTGSTATMIF
                     GVAALVSYISKYMTLTPGDVITTGTPPGVGMARNPRVFLKPGDEISATIAGLGEQRFR
                     IVAEA"
     gene            complement(10165..10980)
                     /gene="thiD"
                     /locus_tag="VFY74_11000"
     CDS             complement(10165..10980)
                     /gene="thiD"
                     /locus_tag="VFY74_11000"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX5932257.1"
                     /translation="MTAIALTIAGSDSSGGAGIQADLKTFSALGVYGASAITALTAQN
                     TLGVEAVHAVPPDFIGKQIEAVARDLDVGAVKIGMLATSAIIEAVAEGLRSFPGVPVV
                     LDPVMVAASGDPLLDEDAVETLRAILVPHATLITPNLPEAAKLLGATQARNEREMSAQ
                     AEALQRLGAQAVLIKGGHAEGDKAVDILVDAEGVLRLEAPRVATRNTHGTGCTLSSAI
                     AAELAKGASLREAVVAAKAYVTAAIEVADQLRIGEGTGPVHHFHALWPRPAKR"
     gene            complement(11026..11772)
                     /locus_tag="VFY74_11005"
     CDS             complement(11026..11772)
                     /locus_tag="VFY74_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX5932258.1"
                     /translation="MRFDLDVTYAGALIAGVLSFLSPCVLPLVPPYLCFLGGTTFDQL
                     TGEDETPSHVYTTVVMSSVAFVLGFTTVFVILGATATAAGQLLAANLPLLSKIAGVII
                     IIAGLHFLGIIHIPILHREARYHADARPAGLVGAYVIGLAFAFGWTPCIGPVLGAILA
                     VAAGEGSMSQGVSLLLVYSLGLGIPFILAAVAIKPFLKAMGRFRRHLGTVEKVLGGFL
                     VLTGLLFLTNSMTLISIWILETFPGLASIG"
     gene            complement(11897..12574)
                     /gene="rpe"
                     /locus_tag="VFY74_11010"
     CDS             complement(11897..12574)
                     /gene="rpe"
                     /locus_tag="VFY74_11010"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5932259.1"
                     /translation="MAPDIIIAPSILSADFARLGEEVRAVDRAGCDWIHVDVMDGHFV
                     PNITIGPDVVKALRPHSQKLFDVHLMIAPCDPFIEAFAKAGSDLITVHAEAGPHLDRS
                     LQLIRSLGKKSGVSITPATHESAIEYVLDKIDLVLVMTVNPGFGGQAFIASQLDKIAA
                     IRKMIGARPIHLEVDGGINAETAARVVAAGANVIVAGSGVFSGGAYADNIAAIRAAAM
                     RARAAVA"
     gene            complement(12867..>13067)
                     /gene="pgk"
                     /locus_tag="VFY74_11015"
     CDS             complement(12867..>13067)
                     /gene="pgk"
                     /locus_tag="VFY74_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5932260.1"
                     /translation="VARLTEAGKLVSVAGGGDTVRALKEAGAASGFTYVSTAGGAFLE
                     WLGGHELPAVRALARSGARSAT"
BASE COUNT         2435 a         4159 c         4278 g         2195 t
ORIGIN      
        1 cgtcgatctg gaacgcccgc aactcggtgg ccagcgcgta ctggccggtc gaagagctca
       61 ccgctttcac cgcccccgaa ttctggaagg tctcgatgat gcgcgcctgc accaggcgcg
      121 gcaggcggtc gctccaggcg acgcccttgt agtaggacac ctgatcggcc ttcggccgca
      181 ccatcaggcg gttggtctcg agcgcgtgca cggcggtcgg ctcgtagacg acgagctgcc
      241 agggcgcggc acgcggagcg gcggcgaagg agcgcggtgc gacgaggtca taagtggccg
      301 gcggccttcc gcctcccgcc aacgcgcaac cgacgaggcc caggcaaagc aagcctgcag
      361 ccagcgttgg cgctgatcgt gacatgaccg atcgcccccc gggtaggaga cacccccgat
      421 ggccctagac gggctcctta tcttccacgc ggagtgtatt ccgggacctg gcttccacca
      481 agcaaaaaac ccctgggatc agcctcgagc ttctccagaa ttcggttgag ggttcctgcc
      541 gcgcggcgtc cttcacgcat gaaattcccg aattcctgaa gactttctcg cgcctggcgg
      601 ctcaacccct ccatattgtc cccgagcgag acttcgagct tggtggacaa cgacttgaaa
      661 gactctgcac cttccttcac gttactgatg gtcaggccaa tatcgtcgct ttttgcgttc
      721 agcatcgtgg tgaactgttc gacattggtc atcgtggtgt tgatcgactt ctcgttctgg
      781 tcgaggagag cgttcagctt cgaaatgaag acattggcgt tgttcagcgc cgccgagccc
      841 gcctcgaaca cgccctggcc cgatccggga tcggctctta tcaccggaat gggattctcg
      901 gccgtcgcca ccagaatcgg cgaatcgggc gtgcccggcg tgatctggat tccgctccct
      961 cccgtcagcc ccatggattg gatgctcgcg tgcgaattct cgcgcaccgg cgtgtcgtcg
     1021 cgaacgctga cgagcacctg caccttgcgg gcgtcctcgg ggtccagcgc gaaggactgc
     1081 acttcgccga ccctgatgcc gttgaacccc acgctcgagc cggtcgtcag tcccgccacc
     1141 gagctttcga acacgatgga gtagcgtttg cccccgcccg cgaaattgct catccaaaaa
     1201 acgaagatga acgcgagcgc gagcacgccg agcacgaacc ccccgatcat caggtaattg
     1261 gctttggttt ccatcaaacc ggttcctcag aacatgcgac aggtctcaag ccgggatcct
     1321 cgggcgctcg cccggcgatg ccgcgcgggc ccgttcgccg cagaaatatt cctggatcca
     1381 ggggtggttc ggttggcgct gcaactcggc cggcgacccg gtccgcacga tcttcttgtc
     1441 ggcaagcacg gccaccctgt cgcatacggt gaagatcgta tcaaggtcgt gggtcaccat
     1501 gtagaccgtg aggccaagtg tctgctgcaa cttgcgaatc aggtggtcga aatcggtggc
     1561 gccgatcgga tcgagtcccg ccgtgggctc gtcgaggaac acgatctccg gatcgagcgc
     1621 gagcgcccgg gcgagccccg cccgcttgcg catgccgccg gatagttcgg agggaaattt
     1681 gtaggcggcg ttggggggaa gcccgaccat ggagagcttc aggaacgcga gatcgtccat
     1741 catcgcctgc ggcagttcga gatgctcgcg catcggcacc tggatgtttt gcagcacggt
     1801 gagcgaggag aagagcgcgc cgtcctggaa caggatcccc cagcggcgct cgatgtcgag
     1861 gcgatcctgc ggcgacaagg tcttggtgtt ctgaccgaac agcccgacat cgccgacatc
     1921 gggtttgacc aagccggtaa tcacgcgcat gagcaccgac ttgccctgac ccgatccgcc
     1981 gacgatcgcg agaatctcgc cgcgatagac gtcgagattg agcccgtccc aaatgcgatg
     2041 gctgccgaag ctcttggcga gcccgcgcac gcggatgaca acctcgcgtc cgttctcgac
     2101 gatcacacgc cgatccccga cagaaacatg gcgaacacgg cgtccaaaat tatcaccagg
     2161 aagatcgctt tgaccacggc gctggtcacg tgcgtgccga gagattcggc gcttccttcc
     2221 actttgaggc cttcgaggca accgacgagg ccgatgatca cggcggcgaa gggcgccttg
     2281 atcatgccgg ccatgaagtg cttgaagtca accacgtctc gcaacttgtc gatatagttc
     2341 gacgcatcga ggccgaggac ggcgaaagcc atcacgccgc cgccggcaag gcccatcagg
     2401 tcgccgataa aagtgagaag cggaagagcg agcacgagcg cgaggatgcg gggcagcacg
     2461 agcgtgtcca tcgggtcgat gccgagcgtg cgcatggcgt cgatctcctc gcgcatcttc
     2521 atcgagccga tctcggcggt gaaggccgag cccgagcggc ccgccaccat gatggcggtc
     2581 aagagaatgc cgacctcgcg gagcgcgaga atggcgacca tgttcacggc aaagggttcg
     2641 gcgccgaacg gtttcagctg gaccacgccc tgctgcatca ccacggcccc gatcaaaaag
     2701 cagatcagcg cgatgatcgg cacggcgcga aagccgacat ggtcgagatg atgaatgaag
     2761 gacgtgggcc ggaagcgcca aggctggcgg atcatgcgtc cgagcgcgga agtaatttcg
     2821 ccgagaaacg ccgtgagctg gaccgcgtcc cgccagacgc ccgtcagcgc cctggtcatg
     2881 tcatcggtga agaactgaag cggcttccgc ttttcgatcg tcgggcgctc ggcgcccccg
     2941 cggcgccgca cctcatcgat caggatgcgg aagccttcgg tcgctccggc ataacgcgtg
     3001 cggagcccgc tcctctccca ggcggcggcc gtgcgctgta acagcatggc gcccgcggta
     3061 tcgatttcag cgatgccggc aatgtccatc tctccggtga agtccgctgt cggcgggatc
     3121 ggcacccgca ggcgcttgag cgccatgtcg agctgggccg cctcggcgac ggtccaggcg
     3181 ccggtcgcca ccagacgaaa cgtagtgccc ctgagctcga cgcggaagcc gggggcgcgg
     3241 gcttgcgtcg gcaggacaac ctgatggtcg cgcttgagca tcgaagggct tagcctgaag
     3301 ctgttgaccg gtgccgttgg aagtcctctg tttctgaacg aaaatccgga ggaaagaaag
     3361 gcggtatggc gggtctcggg cgcgggctag acggccgcaa atcgcgcgga agcggccttg
     3421 ccctttcggt cgcgactgaa cgctggccga tcaggggcgg ctttgcgatc tctcgtggga
     3481 tcaagcgcga agcggtggtc gtggtcgcga cactcagcga cggcacccat accggccgcg
     3541 gcgaatgcgt accctacggg cgctacggcg agagcgtcga ggcgacggtg gcggcgatca
     3601 gggctcgcgc gccaagggtc gccaacggcg tgagccggac cgagctcctc gaagcgctgc
     3661 cgcccggggc cgcacggaac gcgctcgatt gcgccttgtg ggatttcgag gcgaagagct
     3721 ccggacggag cgcgtcagag cttgcggggc tcggaacgct gaagcccgtg cttacggcct
     3781 ttacgctttc gctcgcctcg ccagaagcga tggcagagaa ggcgcgcgca gcaagcgact
     3841 atcccctgct gaagctcaag cttggcggag agggcgacgc cgagcgcctc gccgccgtgc
     3901 gcgatgccgc gccaagcgcg cggctgatcg ccgatgccaa tgaggcttgg cggcccgaag
     3961 agctggaacg tttgcttgat gccgccgctt tggccggcgt cgagctgatc gaacaaccgc
     4021 ttcacgcatc ggatgacgaa atcttgcagc acatcgagcg ccctgttccg atctgcgccg
     4081 acgagtcggt ccatgatcgc gcctcgctcg aggggatcgc ggcgcgttac gacgcggtga
     4141 acatcaagct cgacaagacc gggggcctga ccgaggcgtt agcggtcacg gcccacgcgc
     4201 gcgcgctcgg gctgaaaatc atggtcggga gcatggtggc aacttcgctc gcaatggccc
     4261 ctgcccttct gttggcgcaa gacgcggatt tcgtcgatct cgacggcccg ctcctgctct
     4321 tgcgcgatcg catccccggg ctcactttcg ttggaggaga gatcctgcct gcgcctcccg
     4381 agctatgggg ctgaccggac cgtcacacga tcagtggccg tgcatcatcg cgccgaccac
     4441 ctgctcgccg cgccagcgct gcgccagcac gtaggcgccg cctgcgcctg ccagtgcaat
     4501 cgccgccatc gcggcataag ccgcaccggc caagctctcg tagagcggac ctgaagcaag
     4561 ggtgaaggtg ccgagcgcga tccccgcgac aaccgcggca tagacgcctt gcgcggtggc
     4621 ggcgcgattc tcgggcacgg catgcgtgag gaaatggatg gcggcgaggt gggccgcgcc
     4681 gaagctcatg gcatgaagtg tctgaagcgg gccgaggatc cagagcggcg gatcaagagc
     4741 catcaggccc cagcgcaagg tcgtcgcgag tcccgcaagc acgagcagcc gcgccgtgcc
     4801 gaaatgggcg atgacgcggc ccgaaaccgc aaagagcgcg acctctgcga tcacgcccac
     4861 cgaccacagt gccccgatcg tgccgtcggg aattccttgc gcgcgccaat ggagcgtgcc
     4921 gaagctgtaa tagagggcgt ggctcgcctg gatcaggctc gtggcaagca gaaacaggag
     4981 aaagagcggc gcgtggacca gccggagggc gtctgcgaat ttgaggccgc gcagcattga
     5041 ggggccgcgt ccctgatcgc cgagctcgcg cggcagaaga tgggcgccga gaacgaggag
     5101 cgctgtcccc gcgacgagaa gcggcaggat cacctgaggt ccaatcttcc cgatgatgac
     5161 accggagctg aggctcgcaa cgatgaagct cagcgagccc cataacctca ctctgccgta
     5221 gtcgagcgca ccctttcgga tcccgctcgt cgcaacggtc tcgaccaggg gcgtgatcgt
     5281 cgtccagttg atggcgagga ggatgctggc gagaagcatc tgccaaaatc cgttcgccgc
     5341 ccaaaacagg agaaacgaaa gcagagatcc ccaagcgagc gcgatgacaa tgttgcgccg
     5401 gctcccggcg agatcggcgg cgaagccgat gaggggcgta aagaagatgc gggtgaaaag
     5461 cggcgcggca agaagcacgg cgatctcgtc gggaccaagg ctccgccaat cgagccagac
     5521 cggcatgaaa gggagatagc acccgaccac gaggaacagc gctgcgtaga ggaagccaag
     5581 gcgccaggcg aagggatgtc cggcccgggc cggcggcggc aattttccca tggcgaaagt
     5641 cccgggtgct tagctcgttt gagtcgtcac tattcgtcat tttatatcag tgacttaaac
     5701 aatccggcgg aaattcttaa ccaccccctt gaaaccggcg gggccggaaa ccatctccaa
     5761 ggagacgcga gttcccccaa agcgtccccc aaactgacgc atgagagtag acggcctggt
     5821 tccccccaac cgggccgttt tcttttgcac ccggcgcttg cggcgcgaaa gccgcgccgg
     5881 cagtcccaaa tgatgagcag cgagcacgtt ctcgaagaat tgccgcgcgg aatgctccca
     5941 actgaagcga agcgcatgcg ctctcgccgc cgcccgatcg agcgcgagag cctcaagggc
     6001 cgccgccctt aaatcggggc caaggacgcc ggcccgcgca tccgtgatga cgtcgagagg
     6061 tccggtggtc ggataggccg cgacgggcag gcctgccgca agcgcttcga ggaggacgag
     6121 gccgaacgtg tcggtcatgc tcggaaacac gaaaacgtcg gcagaggcgt aagcctccgc
     6181 aagcgccacg ccatctcgcg ggccggtgaa cagagcattc ggatagtgac gtttgaggtc
     6241 tctcgcttgc ggcccgtctc ccaccaccac tttccggcct ggcaggtcga gatcgagaaa
     6301 cgccttgacg tttttctcca cggcgatgcg cccgacatag aggaagaccg gcggcgggcc
     6361 gaacagcctg acgttccgcg gcttgaacaa ttcggtatcc acgccgcgcg accacggcat
     6421 caatcgcgtg aagccgcgcg cctcgaggtc ggaggcgagc gacggcgtgg cgacgaaggt
     6481 cccggccgcg ccgttgtgaa accgccgttg cagcgcgtag ccccatgact caggcacagg
     6541 cagccgcgcg gaaagatatt cgggaaagcg ggtgtggtaa ctcgtggtga agggccactt
     6601 ggtcttccgg caatagcggc gcgtcatgaa gccgatcggt ccctccgtgg cgatatgaat
     6661 aaagtcgggg cgcgcggcct cgacgtgtcg tgcgatcgcg cgcggaccgg caagcgccag
     6721 cctgatctcg ggataggtcg gacacgggaa cgtgcggaag tgcgggggcg ctagaaagct
     6781 gagctggaag ccaagcgcgg gcgcctcgga ggcgaggcgc tcataggtcc tgaccacccc
     6841 gttcacttgc gggtgccagg cgtcggttgc gatgagaacc cgcatcatgc ggcagcctcg
     6901 agctcgtcga ccagaagttc gaatctcggc ctgccttcgg gatgccccca acggatgatc
     6961 tcgaagatcc cgtcgtcggt ctcggcgacg gctgtgcggc tctcgaccca gtcgccggta
     7021 ttcacatagg cgatgtcgcc gacctggcgc atggccgcgt ggtgaatatg gccgcagatc
     7081 accccttgcg ctccgcgacg gcgcgcttcg tgcgcgagcg ccgcttcgaa ctcgccgata
     7141 tagttcacgg cccgcttgac cttctgcttg agataggcgg agagcgacca gtagggcagc
     7201 cccagcaggc gacgaatggt gttgaaatgg gtgttggccc aaagcgcaaa cgcgtagctc
     7261 caatcaccga acagtgcgag ccatttggca tagcggacga cgacgtcgaa ctcgtcgccg
     7321 tgcatgatga gatagcggcg cccgtcggcc gccacatgga tcgcgctgcg ttcgatggcg
     7381 acacccccga accttgtccc gcaataatcg cgcatcgcct cgtcgtgatt gccgggaacg
     7441 tagatgatct ccgtcccctt gcgcgcccgg cgcaacagct tttgcaggac atcgttgtga
     7501 gattgcggcc agtagatgcc gcgcttgatg cgccagaagt cgacgatgtc gccgatcagg
     7561 tagattttcg aggcgtcgtg gcagcgcagg aagtcgatga aggcctcggc ctggcaggcc
     7621 ttggtaccca gatgcagatc cgagatgaaa atcgcgcggt agcgcttcat ccgcggttca
     7681 tgcttcacgt cgacgtcagt ccccgccttc atcacgttgc acggggactt acggtgcgtt
     7741 tgtttcaggg ttgtgacatt ggcggacaag gttaagggcg ggccgatgga gcacaacggc
     7801 ctcgtgacgc ggcttgccgg cgcgctcggc tctactgtgg tcgacgcgcg gccacttgcc
     7861 gtcgggttcg gactcatcgg gcttgaaatc aggcttgacg acggcaggca cctcgcggtc
     7921 aaggcgcgcg cgtcaggcca aggccgcgca tccgatctct cgctcgaggg ctacatgctg
     7981 ggcgagctcg cccgtcactc cgatttcccg gttccacgcg tgcatcacgc cgaacccggc
     8041 cttctggcga tggatttcat cgagacggac ggcagcggga tcacgccaag cgtcgaacgt
     8101 catgcgggcg agctgatcgc aaggctgcac gcgaccccgc gcgccgcgtt cggttatgag
     8161 cgcgatacgc tgatcggacc gcttcctcag ccaaacccga tctcgcgaag ctgggtgccc
     8221 ttcttccgcg accagcgtct tcttttcatg gccaagcagg cgcaccttga gggggcgctc
     8281 ccctcatcca tgcttcaccg catcgaacgc ctcgccgggc ggatcggcga ttacatcatc
     8341 gagccggcct ttccgagcct gctgcatggc gacctctgga cggggaacgt tctcgtgcgc
     8401 ggcggaagga tcgccggctt cgtcgacccg gccatctatt gcgggcatcc cgagatcgaa
     8461 gtcgcgttcg cgacactgtt cggcacattt ggggaagctt tcttcgaagc gtacgaaagc
     8521 tcgatgccgc tcgagcccag ctttcgcgag gtccggctag acctctacaa tctctacccg
     8581 cttctggtgc atgtgagact gttcggctcg ggctatctcg ccggcatcga ccggacgctt
     8641 gcgcgggtcg gtctctaggg acagggctcc ggctcgagac aaaaagaacc ggcccggaca
     8701 gtgccgggcc ggtcttccct gcgaaacgag ccgacctcta aaaggcatgg ccttaatagc
     8761 gggcttcggc tcgccccccc atcgtgttcc tgagtgttag attctttggt ttgttacagg
     8821 tccctcttag cgccggtcgg tagtgagtgg gccccccagc cccgtccccc gtctcaattc
     8881 gtgacgagag gctaactagc cccgcgggcg ccttcaacct tgcgccaagc ggtggaatgc
     8941 aaaacggtcg agcactgtgg cacaaatgcc aaaaaacgat ctttgtgcat ttatatcagt
     9001 tacttaggag gcaaacgggc cgacggaaga cggcgcgatt ccgcgcgagg tgaagtgctg
     9061 cctccgacca gcgtcgcgtt tttgcgacca aactttgttg ggtcttgttg cagttcggca
     9121 acgggcttca ttcggccttc gcgaggggcg cgtacgattc gatttgcgcc ggaaggcccc
     9181 tgatgtgcga aaacaccaag gccctcacat tcttggcatt attgcgcgtc cgcgacgatt
     9241 tgcgaagccg ccgggccgtt gccgcccagc agccgcgcgt gcatcatgca aaaatggacc
     9301 ggggaacgat cgcatgaagc ttcttcgtta cggcgcggtt ggacaggaaa agccgggagt
     9361 tgtcgatgct gacggcgcga tccgcgacct gtcgggcgtc gtcgccgacc ttgcgggggc
     9421 cgcgcttgga cggaagagcc tcgatcgcat cagggctctc gatctcgcgg cgttgcctct
     9481 cgttcagggc gcgccccgca tcggccctcc cgtcggcgtg gtgccgaaat ttctcgggat
     9541 cgggctgaac tatagcgacc atgccgcgga gaccggcctg ccgctgcccg aagtcccgat
     9601 cgtcttcgcc aaggcaagct cgtccgtctc gggccccaac gatccggtgc ttgcgccaag
     9661 cgatttcaag cgcatggatt acgaggtcga gctcggcgcc gtcatcggct cacccgccaa
     9721 gaacgtctcc gaggccgagg ctctcgatca cgtcgcgggc tacgcgatct gcaacgatat
     9781 ttccgagcgc agcctgcaga agggcggccc cggcgaatgg atcaaggcca agagctacga
     9841 tacgttcgga ccgctcggac cttggctcgt gaccacggac gagatccccg acccgcaggc
     9901 gctcgatctt gcgctcgacc tcaatggggc ccggatgcag accgggtcga ccgcgacgat
     9961 gatcttcggc gtggccgcgc tcgtgagcta tatcagcaaa tacatgacgc tcacgccggg
    10021 cgacgtcatc accacgggca cgccgcccgg tgtcggcatg gcgcggaacc cgcgcgtgtt
    10081 cctgaagccc ggcgacgaga tcagcgcgac gatcgcaggt ctcggcgaac agcggttcag
    10141 aatcgtggcg gaggcctaga gccttcacct tttcgccggg cgcggccaga gcgcatggaa
    10201 atgatgcacg gggcccgtgc cctcgcctat gcgaagctga tcggcgactt caatcgccgc
    10261 cgtgacatag gccttggcgg cgacgacagc ctcgcggagc gatgcgcctt tggcaagctc
    10321 ggcggcaatc gccgatgaca aggtgcagcc ggtgccatgc gtgttgcgcg tcgcgacgcg
    10381 tggcgcctcg aggcgcaaca cgccctctgc atccacaagg atgtcgacgg ccttgtcccc
    10441 ctcggcatgg cctcctttga tcaacacggc ctgggcgccg agccgctgaa gggcctcggc
    10501 ctgcgccgac atttcgcgct catttctggc ctgggtggcg ccgagaagct tggccgcctc
    10561 gggcaagttc ggggtgatca gggtcgcgtg cggcacgagg atggcgcgaa gcgtctcgac
    10621 ggcgtcctca tcgagaaggg gatcgccgga agccgcgacc atcaccggat cgagcacgac
    10681 cggcacgccg ggaaaggatc ttagcccttc ggccaccgcc tcgatgatcg cgctcgtggc
    10741 cagcatgccg atcttgacgg cgccgacatc aagatcgcgc gccaccgcct cgatctgctt
    10801 gccgatgaaa tccggcggca cggcatggac ggcctcgacg ccgagcgtat tttgcgcggt
    10861 gagcgcggtg atcgcgctcg ccccgtaaac gccgagcgcc gagaaggtct tgaggtcggc
    10921 ctggatgccg gcgccgcccg aggagtccga gccggcgatg gtcagcgcga tcgcagtcat
    10981 ggccatgtca tatcggcgtg cagagcaaaa tgcgagcgcg ccgaatcagc cgatcgaggc
    11041 aagtccaggg aacgtttcga ggatccagat cgaaatcagc gtcatggagt tggtgaggaa
    11101 gaggaggccg gtgagcacga gaaacccgcc gagcaccttc tcgaccgtcc ccagatggcg
    11161 gcggaaccgt cccatcgcct tgaggaacgg cttgatggcg acggcagcga ggatgaaggg
    11221 aatgccgagc ccgagcgaat agacgaggag caggctgacc ccctggctca ttgagccttc
    11281 ccccgcagcg accgccagga tcgcgccgag cacgggccca atgcagggcg tccagccgaa
    11341 ggcgaaggca agcccgatga cataggcgcc gacgaggccg gcaggccgcg cgtcggcatg
    11401 atagcgcgcc tcgcgatgaa ggatggggat gtggataatg ccaaggaaat gcaggccggc
    11461 gatgatgatg atcacgcccg cgatcttgct cagcagcgga agattggcgg cgagaagctg
    11521 gcccgccgcc gtcgcggtcg cgccgaggat cacgaacacg gtggtgaagc cgagcacgaa
    11581 ggcgaccgac gacatcacga ccgtggtgta gacgtgggat ggcgtttcgt cctcgccggt
    11641 gagttggtcg aaggtcgtgc cgccgagaaa gcagaggtaa ggcgggacaa gcggcaggac
    11701 gcagggcgag agaaagctca acaccccggc aatcagcgcg ccggcatagg tgacgtccaa
    11761 atcgaacctc atccgccaat ttgagccgag gctccgggcg cacggcaagc ccgcttactc
    11821 cagacagaat tgcgcgctca atcggcccga tgacaagtca cgttcatgtt aaggggcaag
    11881 cctgcggctc gcggcctcac gcgacggcgg cgcgggcgcg catggcggct gccctgatgg
    11941 cggcgatatt gtcggcgtaa gcgccgccgc tgaagacgcc agagcccgcg acgatcacgt
    12001 tcgctcccgc cgcgaccacg cgcgcggctg tctctgcgtt gatgccgcca tcgacctcga
    12061 ggtgaatggg ccgcgcgccg atcatcttgc ggatcgccgc gatcttgtcg agctggctcg
    12121 cgatgaaggc ctggccgccg aagccgggat tcaccgtcat caccagcacc aggtcgatct
    12181 tgtcgagcac gtattcgatc gcgctctcat gcgtcgcggg cgtgatgctg acgccggact
    12241 tcttgccgag gctccggatg agctggagcg agcggtcgag atgcggcccg gcttccgcgt
    12301 gaacggtgat gagatcggag cccgctttcg cgaacgcctc gatgaaaggg tcgcagggcg
    12361 cgatcatcag atgcacgtcg aagagcttct gcgaatgcgg acggagcgcc ttcaccacgt
    12421 cgggcccaat ggtgatgttg ggcacgaagt gcccgtccat gacatcgaca tgaatccagt
    12481 cgcagccggc gcggtctacg gcccgcacct cttcgccgag cctggcgaag tctgccgaga
    12541 ggatcgacgg cgcgatgatg atgtcggggg ccatcggtcc ttgggccatg ggtccttgcc
    12601 cttgtctcct ggtcacgctc atggccttga gccttgtcct ttcccttcta tgtcactgcc
    12661 gggcgctgcc gggcaatgcc gcaagccggc ataagccacg atccgaagat cgatggttac
    12721 tagattcccg catgggcggg aaacgctttc ctttgtcatt gcccggcagg accgggcaat
    12781 ccagtaatcc atgcctcgga ggacagccaa gcacggtgag tactggattc ccgcttccgc
    12841 cggaatgaca aggtctggcc gcagggctag gtcgccgagc gggccccgct cctcgcaagc
    12901 gcccggaccg ccggcagttc gtggcccccg agccattcga ggaacgcgcc gcccgcggtc
    12961 gagacatagg taaagcccga tgcggcgcct gcctccttaa gcgcccgcac cgtgtcgccg
    13021 ccgccggcga cgctgacgag cttgcccgcc tcggtgagac gtgccac
//
LOCUS       DASUQP010000238         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_528600,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000238 DASUQP010000000
VERSION     DASUQP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528600"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFY74_11020"
     CDS             <1..378
                     /locus_tag="VFY74_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5932261.1"
                     /translation="DAVEFTGSVDKIADGDTFWVCDTTACHKIRVCGINAPERGDPGY
                     AESGIALATVLSGKPVRCVQVGGGTPCDGRSKPTNRDRIVAQCFAEESDIAATMVEGG
                     FACDWIKFSGGHYSQGGKGKFCE"
     gene            646..1593
                     /locus_tag="VFY74_11025"
     CDS             646..1593
                     /locus_tag="VFY74_11025"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5932262.1"
                     /translation="MKLDRETEALLYWTSSATATPLWTLSPEAARAEYRRTHGKTEIA
                     PPPIGEAIDLTVAGPAGPMRLRRYTPLDPGREAEAAILFMHGGGCVLGDIGTHDIFCR
                     ALCHDTRASVFSLDYRLAPEHPFPAAVEDTVAALHWLSREAAGMGLDPERIAIAGDSA
                     GGGLAAVALHETKGALAARVRAQALIYPALDLRGRLPSRKELARQFPIPSDMIGWFFD
                     HYFGTAWPFTDPRASPSLYADYTGLPPALIMTAGHDPLRDEGAEYSAALKAAGVPVDY
                     VCFEGTIHGFMTMGRVLRGVHGKARKCLASWLAARLNGV"
     gene            1618..1743
                     /locus_tag="VFY74_11030"
     CDS             1618..1743
                     /locus_tag="VFY74_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932263.1"
                     /translation="MKLPTGWRYTSLTPETDLVLGAKGTATVVQDELGNTYQKLD"
     gene            1775..>2692
                     /locus_tag="VFY74_11035"
     CDS             1775..>2692
                     /locus_tag="VFY74_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932264.1"
                     /translation="MVLSGLSPAAAFCLTALALLPAASEEAPGATPSETIAAEPAPGG
                     PAKVIVGAYINDIQELDFKANNYVVDLYVWFRWRAPELDPAKTMEFMNRVASGDNVRE
                     ALYETPRVMPDGSRYSIIRYQGRFSTKFALEKFPFDTQLLSVVMEDGVSSDQEQVFVP
                     DGARPVTLDPIITLPGFTVGQPTMTIAPNTYSTNFGDPSAREADTYSRIVLTVPLKRP
                     IVAMSLKTFVPTALIVVCATLVFFVRPRYVEGRIGLGITALLTLVALQLTSSASLPDV
                     DYLMMLDKIYLLAYVFIILRARARGRHVVA"
BASE COUNT          463 a          862 c          849 g          518 t
ORIGIN      
        1 gatgctgttg agtttacggg ttccgtcgat aagatcgcag acggagacac cttttgggtc
       61 tgcgacacga ccgcttgcca caagatcaga gtttgcggga tcaatgcacc tgagcggggt
      121 gatcctggat atgccgagag cggcatagca ctggcaactg tgttgagtgg aaagcccgtt
      181 cgctgtgttc aagttggcgg tgggacacct tgcgacgggc gttccaagcc gacgaaccgc
      241 gacaggattg ttgctcaatg cttcgcggaa gaaagtgata tagccgcgac tatggtcgaa
      301 ggagggtttg cgtgtgactg gattaaattc tcaggcggtc actactcaca aggcggcaag
      361 ggcaagtttt gcgagtgaat gccaacaagt cgcctttcga cctcgcgaaa tacatttgtt
      421 ggcatttgag ttggtatcct cagggcctcg gactaaaatt gggccggaaa accagtcgct
      481 tggctgtcaa ttttgatgcc ggcctggggc accatacact tggcgtgaaa ttgagcgcgc
      541 gaccgcgagc gcccatggcc cggggcgcgg tgtcgagctg atccaggggg cgcattgcat
      601 ggcgcggccg attgatctct cgtttgatgg gccaggatac atttcttgaa gctcgaccgc
      661 gaaactgagg cgctgctcta ttggacgtcg tcggcgaccg ccacgccgct ctggacgctc
      721 tcgcccgagg ccgcgcgcgc ggaatatcgc cgcacgcacg gcaagaccga gatcgccccg
      781 cccccgatcg gcgaggcgat cgatctcact gtcgcgggtc cggccgggcc gatgcggttg
      841 cgccggtaca cgcccctcga ccccggccgc gaggcggagg cggccattct cttcatgcat
      901 ggcggcggct gcgttctcgg cgacatcggc acgcacgaca tcttctgccg cgcgctgtgc
      961 cacgacacgc gcgcaagcgt gttttcgctc gattaccggc ttgcgcctga acatccgttc
     1021 cccgccgcgg tcgaagatac tgtcgccgcg ctccattggc tgtcgcgcga ggcggcggga
     1081 atggggctcg atcccgaacg catcgcgatt gccggcgaca gcgccggcgg cgggctcgcg
     1141 gccgtcgcgc tgcatgaaac gaagggcgcg ctcgcggcgc gggtccgggc gcaggccctg
     1201 atctatcccg cgctcgactt gcgcgggcgc ttgccctcgc gcaaagagct cgccaggcaa
     1261 tttccgatcc ccagcgacat gatcggctgg ttcttcgacc attatttcgg gaccgcgtgg
     1321 cccttcaccg atccgcgcgc aagcccgtcg ctctatgcgg actacaccgg cctcccgccg
     1381 gcgctgatca tgaccgcagg gcacgatccc ttgcgcgacg aaggcgcgga atactccgcg
     1441 gcgctcaagg cggcgggcgt gccggtcgac tacgtgtgct tcgagggcac gatccacggc
     1501 tttatgacga tgggccgggt gctgcgcggc gtccatggca aagcgcgcaa gtgcctcgcc
     1561 tcctggctcg ccgcgcgcct caatggcgtg taaacgagaa cgccgaacgg cgaccggttg
     1621 aaattgccca cgggctggcg ctacacatcg ctgaccccgg agacggatct cgtccttggc
     1681 gccaagggaa cggcgacggt ggttcaggac gagcttggga acacctatca gaaactcgat
     1741 tgaccttgtt ccgcgccctg ccctggccgg acccatggtg ctgtcaggtt tgagccccgc
     1801 cgcggctttt tgcctgacgg cgctcgcgtt actgcctgca gcaagcgaag aggcgccggg
     1861 ggcaacgccc tccgaaacca tcgcagcgga gccggcgccg ggcggtcccg ccaaggtgat
     1921 cgtaggcgcc tatatcaacg acatccagga gctcgacttc aaggcgaaca actatgtcgt
     1981 cgatctctat gtctggtttc gctggcgcgc gccggagctc gatcccgcca agacaatgga
     2041 gttcatgaac cgcgtcgcct ctggcgacaa tgtacgcgag gcgctctacg agacgccgag
     2101 ggtgatgccg gacggcagcc gctattccat catccgttac cagggccgct tttcgaccaa
     2161 gttcgcactg gagaaatttc ctttcgacac gcaactcctc agcgtggtga tggaggacgg
     2221 ggtctcgagc gaccaggaac aggtgttcgt gcccgacggc gcgcgccccg tgacgctcga
     2281 tccgatcatc actctgccgg ggttcaccgt tggtcagccc acgatgacca tcgcgcccaa
     2341 tacctattcg acgaatttcg gcgatccctc ggcgcgcgag gctgacacat attcgcgcat
     2401 cgtgctcacc gtgccgctca agcggcctat tgtcgccatg tcgctcaaga ccttcgtgcc
     2461 gaccgccttg atcgtggtct gcgcgacgct cgtgttcttc gtgcggccgc gctatgtcga
     2521 gggaaggatc ggtctcggca tcacagccct gctcacgctg gtcgcgctgc agctcacctc
     2581 cagcgcatcg cttcccgacg tcgactacct catgatgctc gacaagatct atctgcttgc
     2641 ctacgtgttc atcatcctgc gcgctcgcgc gcgtggtcgc cacgtcgtgg cg
//
LOCUS       DASUQP010000239         7184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1209672, whole genome shotgun sequence.
ACCESSION   DASUQP010000239 DASUQP010000000
VERSION     DASUQP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209672"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFY74_11040"
     CDS             complement(<1..289)
                     /locus_tag="VFY74_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5932265.1"
                     /translation="MKRAAIVPHGSARARFILRLAALFIGSSPLALAQAEEGLTNPVI
                     GQPAAISAGRDIYRSKCLVCHHSAGARGPNIFATKLSDHHFAETVIHGFGHR"
     gene            complement(292..1224)
                     /locus_tag="VFY74_11045"
     CDS             complement(292..1224)
                     /locus_tag="VFY74_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5932266.1"
                     /translation="MSAHEGDNLWTESMLAFDPDTGKIKWGFQYTPNDPWDYDGMSTP
                     ILIDAKIDGQDRKLAAVSNRNGFFYAIDRTNGQFVYAFPLVEGINWTSGLDPKTGRPT
                     INEALKPKIGGPQVKTTVPGLEGGTNWFPPAYDPELGLFFVAVNQWGMGLTAWEKAKL
                     IYKPGDFYQGADYQMYRMGETIGRIKAIDVADKKVVWEVESALPLFSGMLVTKGGVLF
                     TGDLRGRFLAYDAKSGKELWKFQTGSGINASPITYELDGKQYVAILSGLGGDPTFYYS
                     APKGGMLWVFAIDGKVDEGDLYNTQVIEKMLPKQ"
     gene            complement(1205..1441)
                     /locus_tag="VFY74_11050"
     CDS             complement(1205..1441)
                     /locus_tag="VFY74_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932267.1"
                     /translation="MIIGVSGGEYGVRGYIKSFNAKTGALEWQTFTVPAPGEPGSETW
                     PKDDTWKSGGGPTWLTGSYDAETNTLYWGVGPRG"
     gene            complement(1416..1964)
                     /locus_tag="VFY74_11055"
     CDS             complement(1416..1964)
                     /locus_tag="VFY74_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5932268.1"
                     /translation="MTVALPSIAHAKPVTTEDLLKAQENAGEWLTYGRDYRHWRYSPL
                     AEITPDNAAKLSPVWSMSTGGQFGGLESTPLFRDGVLYFSADYARVFAVDAKSGNIVW
                     FYEPEYEQGLTAMLCCGPIHRGLALKDDMVFVARLDAKLVALNQADGKVVWEAKIDEE
                     WRHHEFGAARRQGPRDHRRVGR"
     gene            complement(2086..3219)
                     /gene="proB"
                     /locus_tag="VFY74_11060"
     CDS             complement(2086..3219)
                     /gene="proB"
                     /locus_tag="VFY74_11060"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5932269.1"
                     /translation="MAAMRGEWANAKRIVVKIGSSLLVDGASGALKADWLDALADDIA
                     ALREAGKDVIVVSSGAIALGRNALDLPKGALKLEDSQAAAAVGQIDLAHAYEGAFRAR
                     SLVAAQVLLTLGDTEERRRYLNARSTMTTLLKLGAVPVVNENDTVATTEIRYGDNDRL
                     AARVASMMSADCLVLLSDVDGLYTAPPASRKDARRLEEVTDITPEIEAMAGDAGSELS
                     KGGMVTKIEAGKIAVSGGTHMVIASGRVMHPLKALAKEGAGTWFLARQDPVAARKRWI
                     AGTLEPKGAVTVDQGAAKALGSGKSLLPAGVTKVEGNFERGDAVVIRNGEGVEIGRGL
                     TAYDHEEAKAIIGRKSGEIGEILGYFGRAELIHRDDMVLQRKT"
     gene            complement(3207..4256)
                     /gene="obgE"
                     /locus_tag="VFY74_11065"
     CDS             complement(3207..4256)
                     /gene="obgE"
                     /locus_tag="VFY74_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX5932270.1"
                     /translation="MQFLDQAKVYVRSGDGGNGCVSFRREKFVEFGGPDGGDGGKGGD
                     VIIACVANLNTLLDYRYKQHFKAKKGGHGMGQNKTGAKGADVVLAVPPGTEILDQDGE
                     ALIADLVSPGDRIVLLRGGNGGFGNARFKSSTNQAPRHANPGQKGEEMHIWLRLKLIA
                     DAGIIGLPNAGKSTFLAAVSAAKPKIAEYPFTTLHPNLGVVRIDGTDFVLADIPGLIE
                     GAHEGAGIGDRFLGHVERTAVLLHLVDATLDDVADAYTIVRGEVEAYGAGLEDKPEIV
                     ALSKADAVPKDELKKKARALKKAAGVPPLIASAASGEGVREVLRALAAEVASVRAKGA
                     KEKRGQASPSRGWRP"
     gene            complement(4511..5041)
                     /locus_tag="VFY74_11070"
     CDS             complement(4511..5041)
                     /locus_tag="VFY74_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5932271.1"
                     /translation="MTIRTPRLLLRAPILSDAPRLALLAGDYDVASMTGTIPHPYSEE
                     MAAEWIASVLAGEEGVVFAIIRSGALIGCTGYRAFGKDHAELGYWIGKPYWGMGYATE
                     AVRALIVHAFETDGFDYLTVGHFSDNPASERIIAKFGFTPQGRIERESAARGAKAPCF
                     TYRLDRAKSLAGSYRR"
     gene            complement(5085..5342)
                     /gene="rpmA"
                     /locus_tag="VFY74_11075"
     CDS             complement(5085..5342)
                     /gene="rpmA"
                     /locus_tag="VFY74_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5932272.1"
                     /translation="MAHKKAGGSSRNGRDSIGRRLGVKKSGGQRVLAGNIIVRQRGTK
                     WHPGDGVGIGKDHTLFALREGEVTYATKQKGRVYVSVKQPN"
     gene            complement(5354..5875)
                     /gene="rplU"
                     /locus_tag="VFY74_11080"
     CDS             complement(5354..5875)
                     /gene="rplU"
                     /locus_tag="VFY74_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5932273.1"
                     /translation="MFAVIRTGGKQYRVAPNDIIEVEKIAGKPGDIVELAEVILLGGD
                     GGPKTGSPTIAGAMVAAEVLAQTRGDKIVVFKKKRRKNYRRKKGHRQALTALRITEIL
                     TDGKKPSKAAPKPEAKKAEKSQAKTEVKAKPEKPEAKQAARKAEAKPAAKRSAKTAAK
                     ASAKKPAAKPKKT"
     gene            complement(6011..6541)
                     /locus_tag="VFY74_11085"
     CDS             complement(6011..6541)
                     /locus_tag="VFY74_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932274.1"
                     /translation="MASLQCVRCVLAVAASITLSATGHAAEESKSETVGPWEIEAIFK
                     GDKFDRCAINRPLQEEIVARFVRTPDGLSLELESPNWKLERGKDYPVKMAMGPLSFDA
                     EVAAEANSVSMDIKDKTFESGLRNASALNVIAAGATIVVPLDKSTVAFERLKQCVEKN
                     DKSVETNPFVAPARQP"
     gene            6741..6830
                     /locus_tag="VFY74_11090"
     tRNA            6741..6830
                     /locus_tag="VFY74_11090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6775..6777,aa:Ser,seq:cga)
BASE COUNT         1195 a         2332 c         2243 g         1414 t
ORIGIN      
        1 atcgatgccc aaagccgtgg atcaccgttt cggcgaagtg gtgatcgcta agcttcgtcg
       61 cgaagatgtt cggtccgcgg gcgccggcgc tgtgatgaca gaccaggcac ttgcttcgat
      121 agatatcgcg accggccgag atggccgcag gctggccgat gacgggattg gtcagcccct
      181 cctctgcctg cgcgagcgca agcggcgacg agccgatgaa caaagctgcg aggcggagga
      241 tgaagcgagc gcgcgctgac ccatgaggca caatcgcggc tcgcttcatg actattgctt
      301 cggcagcatc ttctcgatga cttgcgtgtt gtacaagtca ccttcgtcga ccttgccgtc
      361 gatggcgaac acccaaagca tcccgccctt cggcgccgaa tagtagaagg tcgggtcgcc
      421 tccgagtccg gaaagaatcg caacgtattg cttgccgtcc agctcgtagg tgatcggcga
      481 ggcgttgatg cccgacccgg tctggaactt ccacaattcc ttgccgctct tggcgtcgta
      541 agccaggaag cgaccccgca agtcgccggt aaacaacacg ccgcccttgg tcaccagcat
      601 gcccgaaaac agcggcaagg cactctcgac ctcccaaacc actttcttgt cggcgacgtc
      661 gatcgcctta atgcggccga tcgtttcacc catccgatac atctggtagt ccgcgccctg
      721 atagaaatca ccgggtttgt agatcagctt ggccttttcc caggccgtca ggcccatgcc
      781 ccattgattg accgcgacga agaaaagacc gagttcgggg tcataggcgg gcggaaacca
      841 attggtcccg ccttcgagcc ccgggacggt cgtcttgacc tgcgggccgc cgatcttcgg
      901 cttcagcgct tcgttgatgg tcggccgtcc ggtttttgga tcaagcccgc tcgtccagtt
      961 gatgccttcg acgagaggaa aggcatacac gaattgccca ttggtgcggt cgatcgcgta
     1021 gaagaagccg ttccggttcg aaacggccgc aagcttgcgg tcctgaccgt cgatcttcgc
     1081 gtcgatcagg atcggggtcg acatgccgtc gtaatcccag gggtcattcg gcgtgtattg
     1141 aaagccccac ttgatcttgc cggtgtcggg atcgaaggca agcatggact cggtccagag
     1201 attgtcaccc tcgtgggccg acaccccaat agagcgtgtt ggtctcggca tcgtagctgc
     1261 cggtgagcca cgtcggcccg ccgccgctct tccaggtgtc gtccttgggc caggtctcgc
     1321 taccgggctc gccgggagcc ggaaccgtga aggtctgcca ttcgagcgcg cccgtcttgg
     1381 cattgaagga tttgatatat ccgcggacgc catactcacc gcccgacacg ccgatgatca
     1441 cgtggtcctt gacgacgagc ggcgccgaat tcgtggtgac gccattcttc gtcgatcttg
     1501 gcctcccaaa cgaccttgcc gtcggcctgg ttcaaagcca ccagtttggc atcgagccgc
     1561 gcgacgaaga ccatgtcatc cttcaacgcc aagccgcgat gaatgggacc gcagcacagc
     1621 attgccgtta agccctgttc gtattccggc tcgtagaacc agacgatgtt tcccgacttg
     1681 gcatcgaccg cgaacacgcg ggcgtaatcg gcgctgaaat agaggacgcc gtcccggaac
     1741 agcggtgtcg attccaatcc gccgaattgc cctccggtcg acatgctcca tacggggctg
     1801 agctttgcgg cgttgtccgg cgttatttcc gcaagcggac tgtaccgcca gtggcgatag
     1861 tcgcggccat aggtcagcca ctcgccggcg ttctcctgcg ccttgagcag atcctcggtg
     1921 gtcaccggct tggcgtgagc gatcgatggc agcgcgaccg tcatcagagc gagggccaca
     1981 agccacatga gggtcttcat tacgagcctc ccttcgtgct gctgatgcga gccaataggc
     2041 gcatgctaga taaggacggg cgaagcggca acgtggtcgg agccattatg tctttcgctg
     2101 tagcaccata tcatcgcgat ggatcaactc cgcgcggcca aaatagccga gaatctcccc
     2161 gatttcaccg ctcttgcggc cgatgatggc cttggcctct tcgtggtcgt aggcggtcag
     2221 gccgcggccg atctcgacgc cttcgccgtt cctgatgacg accgcgtcgc cgcgctcgaa
     2281 gttgccctcg actttggtga cgcccgccgg caacaggctc ttgcccgagc cgagcgcctt
     2341 cgccgcgccc tgatcgacgg tgacggcgcc cttcggctcg agcgtgcccg cgatccagcg
     2401 cttgcgcgcg gccaccgggt cctggcgcgc caggaaccag gtcccggccc cctccttcgc
     2461 caaggctttt agcggatgca tcaccctgcc cgaggcgatg accatatgcg tgccgcccga
     2521 caccgcgatc ttgcccgcct cgatcttggt caccatcccg cccttcgaca gctccgagcc
     2581 ggcatcgccc gccatcgcct cgatctccgg cgtaatgtcc gtcacctctt cgagccgccg
     2641 cgcgtctttg cgggaagcgg gcggcgcggt gtagagcccg tccacgtccg acagaagaac
     2701 gaggcaatcg gcgctcatca tcgaggcgac ccgcgccgcg agcctgtcgt tgtcgccata
     2761 gcggatttcc gtcgtcgcca ccgtgtcgtt ctcgttcacg accggcaccg cgccgagctt
     2821 cagcaacgtc gtcatggtgc tgcgcgcatt gaggtagcgt cttcgctctt ccgtgtcgcc
     2881 gagcgtgagc agcacctgcg cggcgacgag cgatcgcgcg cggaacgcgc cctcataggc
     2941 atgagcgaga tcgatctggc cgacggcggc cgcagcctgg ctgtcctcga gcttaagcgc
     3001 gcccttgggc agatcgagcg cgttgcggcc gagcgcgatc gcgccggaag agacgacgat
     3061 gacgtccttg ccggcctcgc gcagcgcggc gatgtcatcg gcgagcgcat cgagccaatc
     3121 ggctttgagc gcaccgctcg ccccatcgac caagagcgac gagccgatct tgaccacgat
     3181 gcgcttcgcg tttgcccatt cgcctctcat ggccgccatc ctcgcgacgg cgacgcctgg
     3241 ccccgctttt ccttggcgcc ttttgccctg acggaagcga cctcggcggc gagcgcacga
     3301 agaacctcgc gtacgccctc gccgctcgcg gccgatgcga tgaggggggg gacgcccgcc
     3361 gccttcttca aggcccgcgc cttctttttc agctcatcct tcggcacggc gtcggccttc
     3421 gacagtgcca cgatctcggg cttgtcttcg aggccggcgc cgtaggcttc gacctcgccg
     3481 cgcacgattg tgtaggcgtc ggccacgtcg tcgagcgtgg cgtcgacgag atgcaagaga
     3541 acggcggtgc gctcgacatg gccgaggaag cggtcgccga tgccggcgcc ttcatgcgcc
     3601 ccttcgatca ggccgggaat gtcggcaagc acgaaatcgg tgccgtcgat ccgcaccacg
     3661 ccgagattgg ggtggagcgt ggtgaaggga tactcggcga tcttcggctt ggcggcgctg
     3721 acagcggcaa ggaaggtcga ctttcccgcg ttcggaagcc cgatgatgcc ggcatcggcg
     3781 atgagcttga gccgaagcca gatgtgcatc tcctcgccct tctggccggg attggcgtgg
     3841 cgcggcgcct ggttggtcga ggacttgaag cgggcgttgc cgaacccgcc gtttcccccg
     3901 cgcaacagca cgatgcggtc gccgggcgaa acgaggtccg cgatcagggc ctcgccgtcc
     3961 tgatcgagga tctcggtgcc ggggggcacg gcaagcacga catcggcgcc tttggcgccg
     4021 gtcttgttct ggcccatgcc gtgcccgccc ttttttgcct tgaaatgctg cttgtagcgg
     4081 tagtcgagga gcgtgttgag attcgccacg cacgcgatga tgacgtcgcc gcccttgccg
     4141 ccgtcgccgc cgtcagggcc gccgaactcg acgaacttct cgcgccggaa gctgacgcac
     4201 ccgttccctc cgtcgccgga gcgcacatac accttggcct ggtcgaggaa ctgcatggga
     4261 agggcttagc accggcttct ttccaataca ctagctgcga aggccttttg gccgaccccg
     4321 gcccggcttc atccgcagct gacccctgac gccattttcc ggtgaggggc cgaggacaag
     4381 ttgtccgttg gttcctcacc ctgaggaggg cgcggaacgc acccgtctcg aagggtgcgc
     4441 cctgacgtag gtgcgtgctt cgagacgcgc cctctgggcg ctcctcagca tgagggggca
     4501 cccgctagac tcacctccgg taggagccgg cgagcgactt ggcgcggtcg agcctgtaag
     4561 tgaagcacgg ggccttggct cctcgcgccg cgctctcgcg ctcgatgcgc ccttgtggcg
     4621 tgaagccgaa cttggcgatg atccgctcgg aggcagggtt gtcgctgaaa tgcccgacgg
     4681 tgagatagtc gaacccgtcg gtctcgaaag catggacgat gagggcgcgc acggcctcgg
     4741 tggcatagcc catgccccaa taaggcttgc cgatccagta gccgagctcg gcatgatcct
     4801 tgccgaaggc ccgatagccg gtgcagccga tgagcgcgcc cgaccttata atggcgaaca
     4861 ccaccccctc ttcgccggcg agcacgcttg cgatccactc ggccgccatc tcctcgctgt
     4921 agggatgggg gatcgtgccg gtcatgctcg cgacatcata gtcgccggca agcagggcga
     4981 gccggggcgc gtcggagagg atcggcgcgc gcaagaggag ccttggtgtc ctgatggtca
     5041 cgagcaaaat cgacgcgcgc aagcgcgcgc aagatgcgaa aaatctagtt cggctgcttc
     5101 accgagacgt agacgcggcc cttctgcttg gtcgcgtacg tgacctcgcc ctcgcggagc
     5161 gcgaacagcg tatggtcttt gccaatgccg acgccgtcgc ccgggtgcca tttggtgccg
     5221 cgctggcgaa cgatgatgtt gccggcgagc acgcgctggc cgcctgactt cttcacgccg
     5281 aggcgcctac cgatcgagtc gcggccgttg cgcgaggatc cgcctgcctt cttgtgtgcc
     5341 atgtcctcgt cctttacgtc ttcttcggct ttgccgccgg cttcttagcc gaagcctttg
     5401 ccgccgtctt ggccgacctt ttcgccgcgg gcttcgcttc cgctttccgg gcagcctgct
     5461 tcgcctccgg cttctccggc ttcgccttca cctcggtctt cgcttgggac ttctcggcct
     5521 ttttcgcctc aggtttcggc gcggccttcg acggcttctt gccgtcggtc agaatctcgg
     5581 taatccgcag cgcggtgagc gcctggcggt ggcccttctt gcggcggtaa ttcttgcggc
     5641 gcttcttctt gaagacgacg atcttgtcgc cccgtgtctg ggcaagcacc tcggctgcga
     5701 ccatggcgcc cgcaattgtc gggctgccgg tcttgggacc gccgtcgccg ccgagaagga
     5761 tcacctcggc aagctcgacg atgtcgccgg gctttcctgc gatcttctcc acttcgataa
     5821 tgtcgttcgg cgccacgcga tattgcttgc cgcctgtgcg aatgaccgcg aacatgaccg
     5881 ttcccgtccg agttcttggc ttgaaggacc ggcgtttcag cgcgcggacg agccgcttgg
     5941 ccccttgagc ggcgcaatat agaagccaat gacgataagt caatcggcgg tcttcggccg
     6001 aaaaccctcg ctacggctgc cgcgcgggcg ccacgaaggg gttggtttcg acggatttgt
     6061 cgttcttctc gacacattgc ttgagccgtt cgaaggcgac cgtgctcttg tccagcggca
     6121 cgacgatcgt cgctccggcg gcgatgacat tcaatgcgct cgcattgcga agtccgctct
     6181 cgaacgtctt gtccttgatg tccatcgaca ccgagtttgc ctccgccgcg acttccgcgt
     6241 cgaagctgag cggccccatg gccatcttca ccggataatc cttgccgcgc tccagcttcc
     6301 agttcggcga ttcgagctcg aggctcaagc cgtcaggggt acgcacgaag cgcgcgacga
     6361 tctcctcttg cagcgggcga ttgatcgcgc agcggtcgaa cttgtcgcct ttgaagatcg
     6421 cctcgatctc ccaagggccg acagtctcgg acttgctctc ctccgccgca tgcccggtcg
     6481 ctgacagcgt gatggaggcg gcaacggcaa ggacgcaacg aacgcattgc agggaagcca
     6541 tcaatgcagc tctgcgcata aaggaccccc gtcgagagtg ctgaggcaat ataacgcaac
     6601 cgatgcacac cggttcctga gcggacatgg ccgacccgac gagcggccgg ctgaggcgcc
     6661 gccggcgtga aaggaaaatc ggatcccgcg cggcgccaaa ggttgccgga agcggatcgg
     6721 caagctacat gagccaggac ggagaggtgc cggagtggtc gaacgggacg gtctcgaaaa
     6781 ccgttgtacg ggtaaccgta ccgtgggttc gaatcccacc ctctccgcca tcaactaagc
     6841 actgatatag caggtgtttc ctgccttggc ggggtgtaaa ttgtctggcg ctttccgggc
     6901 cttgcacgcg gacctcgttg agcgcggtag tcagagactc ttttagtgcg ccaattgacc
     6961 gtctctgcag gcctcgtctc ttataggatg cgtttgcagt accaccgtct cgcggcctaa
     7021 ccggaaattc ttggtgagtg agcgagacgc tagaggggga gacgcgcgtg agcagactat
     7081 tgatgctcct caagggcaga ggagttcgcg ctgtgctgtc caatccagag gcaggcgggt
     7141 atcgtccaca gtcggttggc ggtgaggtgt ggaggttgcc ttcc
//
LOCUS       DASUQP010000240         6466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_665417,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000240 DASUQP010000000
VERSION     DASUQP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665417"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFY74_11095"
     CDS             complement(<1..355)
                     /locus_tag="VFY74_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX5932275.1"
                     /translation="MNTKKAIAEFIGTFALVLIGCGAVAISGLGSEQSAIAIFGIAAA
                     FGLTIVAMAYGIGQVSGCHINPAVSLGVLVAGRMTTSDFITYVIAQVLGAIAGAVVLY
                     LILLGKASGWDGGLGQ"
     gene            583..1431
                     /gene="kdsA"
                     /locus_tag="VFY74_11100"
     CDS             583..1431
                     /gene="kdsA"
                     /locus_tag="VFY74_11100"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX5932276.1"
                     /translation="MNEAIKAAPRAVVAAQSAKFGNALPLALIAGPCVLESRAHALET
                     ARALKEIAARLKIGLVYKSSFDKANRTSAASARGLGLDGALPIFAEIRDSLGLPVTTD
                     VHEAEQCAAVADAVDMLQIPAFLCRQTDLLIAAAQTQKPVNVKKGQFLAPWDMKNVVA
                     KLIGAGNPKVLLTERGVSFGYNTLVSDMRALPILAETGAPVIFDATHSVQQPGGQGTA
                     SGGERRFVPVLARAAVAVGVAGLFIETHQDPDRAPSDGPNMLPLSALEPLLKELIEID
                     RLVKRR"
     gene            complement(1540..2001)
                     /gene="queF"
                     /locus_tag="VFY74_11105"
     CDS             complement(1540..2001)
                     /gene="queF"
                     /locus_tag="VFY74_11105"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX5932277.1"
                     /translation="MARKISGLKQLGAETPIPKTPAEAELERVSNPHPDTAYVARFTC
                     PEFTCLCPITGQPDFAHFVIDYAPRAWLLESKSLKLYLASFRNTGAFHEDATIAIGKR
                     VVDLIDPAWLRIGGYWYPRGGIPIDVFWQHGTPPDGVWIPDQGVSPYRGRG"
     gene            complement(2007..2387)
                     /locus_tag="VFY74_11110"
     CDS             complement(2007..2387)
                     /locus_tag="VFY74_11110"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX5932278.1"
                     /translation="MRIYKEFGFEAAHFLPSAPPGHPNSRIHGHSFRVRVTIDGEPDG
                     KTGLVMPLDDVEAAMGDAREALDHRFLNEVEGLSQPTLERVAIWLWDRLHNRLPGLAE
                     IEVARDSCHEGCVYRGPRQRLAAE"
     gene            complement(2427..3062)
                     /gene="queE"
                     /locus_tag="VFY74_11115"
     CDS             complement(2427..3062)
                     /gene="queE"
                     /locus_tag="VFY74_11115"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX5932279.1"
                     /translation="MYAVKEIYYTLQGEGMQTGRAAVFCRFAGCNLWSGREEDRADAT
                     CNFCDTDFVGTDGPGGGKFAQAPDLARACRGAWRGNSGTRPYVVLTGGEPMLQVDEKL
                     IAALHAASFEVAIETNGTLPVPRAIDWICVSPKGEAPLIQRSGDELKLVYPQAELDPR
                     SFEDLDFAHFLLQPKDDGAKSASHLAAAIFYCRAHPKWRLSLQTHKLLGLP"
     gene            complement(3145..3879)
                     /gene="queC"
                     /locus_tag="VFY74_11120"
     CDS             complement(3145..3879)
                     /gene="queC"
                     /locus_tag="VFY74_11120"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX5932280.1"
                     /translation="MTKEVSGSALVLFSGGQDSTVCLGWALDRYARVETAGFDYGQRH
                     GVEMECRQRVRDDIARRFPAWGTRLGPDHVIDISSFGAIGETALTSDAEIEMLASGLP
                     STFVPGRNLLFFVYAAALGYRRGLFTLVGGMCETDFSGYPDCRDATLRTLQEAIRLGT
                     EIPFVIEAPLMRLTKAETWTLAERLGGEAFVALIVEDTHTCYRGERGLRHDWGYGCGT
                     CPACQLRAKGFAEWQASRAGLQAASR"
     gene            complement(3876..4235)
                     /locus_tag="VFY74_11125"
     CDS             complement(3876..4235)
                     /locus_tag="VFY74_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5932281.1"
                     /translation="MPDTQTYGGGCHCGRVRYQVTTNLEPVYSCNCSICQKRGALWTY
                     VTPAEFKLLSGQDELSDYQFNKKAIHHLFCRNCGISSFATGRLEDGSEGIGINVRCLD
                     GVEVEALALTPFDGKSL"
     gene            4349..5629
                     /gene="eno"
                     /locus_tag="VFY74_11130"
     CDS             4349..5629
                     /gene="eno"
                     /locus_tag="VFY74_11130"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5932282.1"
                     /translation="MTAIATIMGREILDSRGNPTVEVDVVLKDGHHGRAAVPSGASTG
                     AHEAHELRDGGTRYGGKGVKKAVEAVNGEVRKALAGFDAGDQRGLDQRLRDLDGTQNK
                     KRLGANAILGVSLAAAKAEASAQGLPLYRSIGGEGATLLPVPMMNIVNGGAHADNPID
                     FQEFMIVPVGAASIAEAVRMGAEIFHALRKALKSAGHNTAVGDEGGFAPNLASTRDAL
                     DFVMAAIREAGYSPGDDVYLALDVAATELFDKGRYALKGENRTLTSDQLIAYYGELVA
                     NYPIFSIEDGMSEDDWDGWAGLTRALGDRVQLVGDDLFVTNVARLKEGIDRGIANAIL
                     VKVNQIGTLTETLDAVRMAQDAGYAAVMSHRSGETEDATIADLAVATNCGQIKTGSLA
                     RSDRVAKYNQLIRIEEELGNKARYAGRAVLKRGR"
     gene            5666..5992
                     /locus_tag="VFY74_11135"
     CDS             5666..5992
                     /locus_tag="VFY74_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5932283.1"
                     /translation="MRFTAFIFPLLACGAAGYFAYHLQTGDHGLEARAKLQGRKEVLE
                     GELAGLKEVRLRLERDVSLLRPESLDPDMLDERARAILNLAHEDDLIMLKRRHAVPAP
                     ATTKKR"
     gene            6207..>6466
                     /locus_tag="VFY74_11140"
     CDS             6207..>6466
                     /locus_tag="VFY74_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5932284.1"
                     /translation="MLLIRRFEEKAGQLYGMGYIGGFCHLYIGQEAVVAGMQMTISEG
                     DQVITAYRDHGHMLAADMEPKRVMAELTGRRHGYAKGKGGSM"
BASE COUNT         1200 a         2079 c         2119 g         1068 t
ORIGIN      
        1 tttgcccgag ccctccgtcc cagcccgagg ccttgcctaa gagaatgaga taaaggacga
       61 ccgcgccagc gatggcgcca agaacctgag cgatcacata agtgatgaag tccgatgtgg
      121 tcatccgacc cgcgaccagc acgccgaggc tcaccgccgg attgatatgg caaccggaaa
      181 cttggccgat gccataggcc atggccacga tggtcaagcc gaaggcggcg gcgatgccga
      241 aaatcgcgat ggcgctctgc tcgctgccca atcctgagat ggcgacggcg ccgcaaccga
      301 taagcacgag cgcaaaggtg ccgatgaatt ccgcgattgc ttttttcgta ttcatgacgg
      361 tatccccctt gtaggcgcgc catgatgcca gacgagacga cgcgagccta gggagcaatg
      421 gtcaaaccat gcttgggaca caaccggctt tacggatcgt tttgcgcgga acgctggtgc
      481 gatctgcttc tcacctgtca cattattggt cgtgatgaca gccggcggcg cgctcgccct
      541 cacccagttg acgcctgagc ctcggggtcc catggtccgg ccatgaacga agccatcaag
      601 gcggcaccga gagcggtcgt cgcggctcaa agcgcgaagt tcggcaatgc cctgccgctc
      661 gcgttgattg ccggcccttg cgtgcttgag agccgcgcgc acgcgctcga gacggctcgt
      721 gcgctgaagg agatcgcggc gaggctcaag atcggcctcg tctacaagag ctccttcgac
      781 aaggcgaacc gcaccagtgc cgcaagcgcc cgcggtctcg ggctcgatgg agcgctcccc
      841 atcttcgccg agatcaggga cagcctcggc ctccctgtca cgaccgacgt gcatgaggcc
      901 gagcaatgcg cggccgtggc cgatgcggtc gacatgctgc agatcccggc gttcctctgc
      961 cgccagaccg atcttctgat cgccgctgcg cagacgcaga agccggtcaa cgtcaagaag
     1021 gggcagttcc tcgcgccgtg ggacatgaag aacgtcgtgg cgaagctcat cggcgcgggc
     1081 aatcccaagg tgcttctcac cgaacgcggc gtctccttcg gctacaacac gctcgtctcg
     1141 gacatgcgtg cgctgcccat cctcgccgag accggcgcgc cggtgatctt cgacgccaca
     1201 cattcggtgc agcagccggg agggcagggg acggcgagcg gcggcgagcg ccgcttcgtg
     1261 cccgtgctcg cgcgcgcggc ggtcgccgtc ggcgtggctg ggcttttcat cgagacgcat
     1321 caggaccccg accgcgcgcc ctcggacggc ccaaacatgc tgccgctctc agcccttgaa
     1381 cctttgctca aggagctgat cgagatcgat cgcctggtga agcgccgcta acgataatcc
     1441 ctcttgcgag tgtcaccacg tcattccggc gaagccggaa tccagaagag gcatgcgctg
     1501 cgcttgtgac tctggatacc ggcttccgcc ggtatgacgc taaccccggc cgcgataggg
     1561 cgagacccct tggtcgggaa tccagacgcc gtcaggcggc gtaccgtgtt gccagaacac
     1621 gtcgatggga atgccgccgc gcggatacca atagccgccg atcctgagcc aggcgggatc
     1681 gatcagatcc acgacacgct tgccgatggc gatggtcgca tcctcgtgga aggccccggt
     1741 gttgcggaaa ctcgcgagat agagcttgag cgatttcgat tccagcagcc aggcgcgcgg
     1801 cgcatagtcg atgacgaaat gcgcgaagtc gggctgaccg gtgatgggac agaggcaggt
     1861 gaattccggg caggtgaagc gggcgacata ggccgtgtcg ggatgcgggt tcgacacgcg
     1921 ttcgagctcg gcttcggccg gcgtcttcgg tatcggcgtc tcggcgccga gctgcttcag
     1981 gccgctgatc ttgcgcgcca tcgtccttac tccgccgcga gccgctgacg cggcccgcga
     2041 tagacgcagc cttcatgaca gctatcgcgc gccacttcga tttcggcgag cccgggcagc
     2101 cgattgtgca gacggtccca cagccatatc gcgacccgtt ccagcgtcgg ctgggacagt
     2161 ccttcgacct cgttgaggaa gcggtgatcg agcgcttcgc gcgcatcgcc catcgcggcc
     2221 tcgacatcgt ccaaaggcat caccagaccc gtcttgccgt cgggctcgcc gtcgatggtc
     2281 acgcgcactc tgaaggaatg tccgtgaatc cgcgaattgg ggtggcccgg gggcgccgaa
     2341 ggaaggaaat gagcggcctc aaagccgaat tctttgtaga tgcgcatcgt agtcgtttag
     2401 gcgattttgc ttggccaagc cagggttcag gggagcccga gcagcttgtg ggtctggagg
     2461 ctcaggcgcc atttcggatg ggcgcggcaa tagaagatgg cggcggcaag atggctcgcc
     2521 gatttcgcgc cgtcgtcctt gggctggagc aggaaatggg cgaagtcgag gtcctcgaag
     2581 ctgcgcgggt cgagttcggc ttgcgggtag accagcttca gctcatcgcc cgagcgctga
     2641 atgagcggcg cctcgccctt cgggctcacg cagatccagt cgatcgcgcg cggcacgggc
     2701 aaagtgccgt tggtctcgat cgcgacctcg aaacttgcag catgcagcgc cgcgatcagc
     2761 ttctcatcga cctgaagcat cggctcgccg ccggtgagca cgacataggg ccgcgtgccc
     2821 gaattgccgc gccaagcgcc gcggcaggcg cgggcgaggt cgggggcttg cgcgaacttc
     2881 ccgccacccg ggccgtcggt cccgacgaag tcggtatcgc agaaattgca cgtggcatcg
     2941 gcgcggtcct cctcgcgccc cgaccagaga ttgcagccgg cgaagcggca gaacacagcc
     3001 gcgcgcccgg tctgcatccc ttcgccctgg agcgtgtaat agatttcctt gaccgcatac
     3061 atggccggaa cttggtcctt aagcgcgccg agggcaagag tatcgtcgtt ctcgccggag
     3121 cggcaatgca gcgtccggcg tagttcagcg tgaagcggct tgaaggccgg cgcgcgaggc
     3181 ctgccactcg gcgaagccct tggcgcgcag ctggcaagcc gggcaggttc cgcacccata
     3241 accccaatcg tggcgaagcc cgcgttcgcc gcgatagcag gtgtgggtgt cctcgacgat
     3301 aagagcgacg aaggcctcgc cgccgagccg ctcggcaagc gtccacgtct ccgccttggt
     3361 cagccgcatc agcggcgcct cgattacgaa gggaatttcc gtgccgagcc tgatcgcctc
     3421 ttgcagcgtc cggagcgtcg cgtcgcggca atcgggatag cctgagaaat ccgtctcgca
     3481 catgccgccg accagcgtga aaagtccgcg ccggtagccg agcgcggcgg catagacgaa
     3541 gaacaggaga ttgcgacccg gcacgaaggt cgagggcagg ccggaggcga gcatctcgat
     3601 ctcggcgtcg gaggtcagcg ccgtttcgcc gatcgcgccg aagctcgata tgtcgatgac
     3661 atgatcgggc ccgagccgcg tgccccaggc agggaaacgg cgcgcgatgt cgtcccggac
     3721 gcgctgccgg cattccatct cgacgccgtg tcgctggccg tagtcgaaac ccgccgtctc
     3781 gactctggcg tagcggtcga gcgcccagcc gaggcaaacg gtcgagtcct gccctccgga
     3841 aaacagcacc aacgccgagc ctgagacctc ttttgtcata ggctcttgcc gtcgaagggc
     3901 gtgagcgcca gcgcctcgac ctcaacgccg tcgagacaac gcacattgat gccaatgccc
     3961 tccgaaccgt cttccaagcg tcccgtggcg aaggacgaga tcccgcagtt ccggcagaac
     4021 agatgatgga tggctttctt gttgaactga tagtcggaaa gctcgtcttg tccggaaagc
     4081 agcttgaatt cggccggcgt cacataggtc caaagcgcgc cgcgcttctg gcagatcgag
     4141 cagttgcacg agtacacggg ctcgagattg gtcgtgacct gatagcgaac gcggccgcaa
     4201 tggcagccgc cgccataggt ttgcgtgtcg ggcattgaag cgatctcctt acgccaaaac
     4261 gctttgcact cccccttgaa cccgatagaa acccgcccgg gccgagcgtg tcatgggcgc
     4321 gtgcggcaat caacccgaag ggcatatcat gaccgcaatc gccaccatca tgggccggga
     4381 aattctcgat agccgcggca acccgaccgt tgaggtcgat gtcgtgctca aggacggtca
     4441 tcatggtcgc gccgcggtcc cttccggcgc ctcgaccggc gcgcacgagg cgcatgagct
     4501 tcgcgacggg gggacccgtt acggcggaaa gggcgtgaag aaggcggtcg aagcggtcaa
     4561 tggcgaggtc cgcaaggcgc tcgccggctt cgacgccggc gaccagcgcg ggctcgacca
     4621 gcgcttgcgc gatctcgacg gcacgcaaaa caagaagcgg ctcggcgcca acgccattct
     4681 cggcgtctcg cttgccgcgg caaaggccga ggcgagcgcg caaggcttgc cgctctatcg
     4741 ttctatcggc ggcgaaggag cgacgctgct gccggtgccg atgatgaaca tcgtcaatgg
     4801 cggggcccat gccgacaacc cgatcgactt ccaggaattc atgatcgtgc cggtcggcgc
     4861 ggcaagcatc gccgaggccg tgcgcatggg cgcagagatt ttccatgcgc tcaggaaggc
     4921 gctgaaaagc gcgggccaca acacggcggt cggcgacgag gggggctttg cgcccaatct
     4981 cgcctcgacc cgggacgcgc tcgacttcgt catggccgcg atcagagaag cgggctacag
     5041 ccccggcgac gacgtctatc tcgcgctcga cgtcgctgcg acggagctgt tcgacaaggg
     5101 ccgctatgcg ctcaagggcg agaacaggac gctgacttcg gaccagctca tcgcctatta
     5161 cggcgagctt gtcgcgaact acccgatctt ttccatcgag gacgggatga gcgaggacga
     5221 ttgggacggc tgggcgggcc tgacccgggc gctcggcgat cgcgttcagc tcgttggcga
     5281 cgatctcttc gtcaccaatg ttgcgcgcct gaaggagggc atcgaccggg gcatcgccaa
     5341 tgccatcctg gtcaaggtca atcagatcgg aacgctgacc gagacgctcg atgccgtgcg
     5401 catggcgcag gatgcgggct atgccgcggt gatgtcgcac cggtccggcg agacggagga
     5461 cgccaccatc gccgatctcg ccgtcgccac caattgcggg cagatcaaga ccgggtcgct
     5521 cgcacggtcg gaccgcgtgg cgaaatacaa ccagctcatc cgcatcgagg aagaacttgg
     5581 aaataaggcc cgctatgccg ggcgcgccgt gctgaagcgg gggcggtaac gcccgcttaa
     5641 cggctcgcgc gcagactccc ggaccatgcg gttcacggct ttcatctttc cgctcttggc
     5701 ctgcggggcg gccggctatt tcgcctatca tctccagacc ggcgatcatg gtctcgaggc
     5761 gcgcgccaag cttcaagggc ggaaggaagt gctcgagggc gagctcgcgg gcctgaagga
     5821 agtcaggctg aggctcgagc gcgatgtctc cctgcttcgc cccgagagcc tcgatcccga
     5881 tatgctcgat gagcgcgcgc gggcgatcct caatctcgcg cacgaagacg acctcatcat
     5941 gctcaagcgc cgccacgcgg tgcccgctcc ggccacgacg aaaaagcgct aaatccgttc
     6001 aggggatttc cggctcgtgc tagcctcggg acgcgaatcc gaggacacgc gcgcgtgtgc
     6061 agcgaggagc aaggaagcac gtgaccacgc caagccttaa ccccggcgcc gcaaatctct
     6121 ggctcggcga ggggaatgcg ggcgcgccga acctgaccgg gcggccgggc ctcacgcccg
     6181 agcaggagcg cgtctcctat cgcctcatgc tgctcatccg cagattcgag gagaaggcgg
     6241 gacagcttta cggcatgggc tatatcggcg gcttctgcca cctctatatc ggccaggagg
     6301 cggtcgtggc cggcatgcag atgaccattt ccgaaggcga ccaggtcatc accgcctatc
     6361 gcgatcacgg acacatgctt gccgccgaca tggagccgaa gcgcgtgatg gccgagctga
     6421 ccgggcgccg ccacggctac gccaagggca agggcggctc catgca
//
LOCUS       DASUQP010000241        16441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_392688,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000241 DASUQP010000000
VERSION     DASUQP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 16441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16441
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392688"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..160
                     /locus_tag="VFY74_11145"
     tRNA            84..160
                     /locus_tag="VFY74_11145"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:118..120,aa:Asp,seq:gtc)
     gene            383..1360
                     /locus_tag="VFY74_11150"
     CDS             383..1360
                     /locus_tag="VFY74_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEX5932285.1"
                     /translation="MATIAGRSMDWNDDTRTNLWVFPRGMQRDGAAGPNSAKWTSKYG
                     SLGTAVYDAAVADGMNEKGLVASLLYLADADYGKADGKPMLSISAWPQYVLDNFATVA
                     EAVETLRTEPFRLGAPAMPTGDAGSVHLAISDPTGDSAIFEYIGGKLVIHHGRQYQVM
                     TNEPPFDQQLALNAYWQEIGGATFLPGTIRPADRFVRAYYLVTAIPKSTVPGIIAAVP
                     DGTYRNQAVASVASVLRAVSVPFGMSSPGEPNVASTLWRTWADQTNLVYFFDSATSPN
                     TFWVPLADLDFGEGAPVKKLTLTGGKVYSGNTADKFEPAKPFAFMPYAS"
     gene            complement(1411..1683)
                     /locus_tag="VFY74_11155"
     CDS             complement(1411..1683)
                     /locus_tag="VFY74_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932286.1"
                     /translation="MIKIWMDFALASARLCHEAQEVMSLRLKKLAQGGSRSRTEAQLM
                     VTEKGFALAEALGTLATGGSLHKVLRRYRGHVRTNKRRLSRPKRTS"
     gene            complement(1751..1945)
                     /locus_tag="VFY74_11160"
     CDS             complement(1751..1945)
                     /locus_tag="VFY74_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932287.1"
                     /translation="MGEEIADVAGDVAAMASKQYGRAQDMAVEAFDDAHAAMKRNPLM
                     AVAFALGLGFLVGVLSRTRR"
     gene            2213..2749
                     /locus_tag="VFY74_11165"
     CDS             2213..2749
                     /locus_tag="VFY74_11165"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5932288.1"
                     /translation="MSKSSNSKVAAKKARGARGKTVAKVAREKARTTRASHRSKPKVV
                     TEAAHVPLRTAPEKAIEQARYRSQHRCSFEAFRDSQVKGTINAIAERSLAQTREAYER
                     SKHTLQAVLASWERSFGAAGQGAIALNRKVIEVADRNVNNAFDLAANLAAAKNFADVM
                     ASHATYWRKQFGNLSARS"
     gene            3040..3405
                     /locus_tag="VFY74_11170"
     CDS             3040..3405
                     /locus_tag="VFY74_11170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5932289.1"
                     /translation="MDQLLQDYLPLVIFIGIAFAFSAALLLAPFAVAVRKPDPEKVSA
                     YECGFDAFGDARMKFDVRFYLVSLLFIIFDLEVAFLFPWAVAFDAIGAFGFWAMMIFL
                     AVLTIGFIYEWRKGALEWD"
     gene            3396..3953
                     /locus_tag="VFY74_11175"
     CDS             3396..3953
                     /locus_tag="VFY74_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX5932290.1"
                     /translation="MGLTLEPKFDKDGRSGGIVTPGATSLGTFSEELADKGFLLTTAD
                     DLINWSRTGSLMWMTFGLACCAIEMMQASMPRYDLERFGFAPRASPRQSDVMIVAGTL
                     CNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCP
                     PTAEALVYGVLLLQKKIRRTGTIER"
     gene            3983..4609
                     /locus_tag="VFY74_11180"
     CDS             3983..4609
                     /locus_tag="VFY74_11180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5932291.1"
                     /translation="MADALDKLAAYLRDRLGPKLSSAELALGELTITVERDQIPEVIR
                     FLRDDSHCRFGSLIDICGVDYPEREERFDVVYHFLSPWLNHRIRVRTMTNAATPVPSI
                     VSLFPGADWFEREAYDLYGILFSGHPDLRRLLTDYGFQGHPLRKDFPLTGFVELRYDD
                     TQKRVVYEPVKLMQEYRSFDFLSPWEGMDNAIPGDEKAGEAPPREGKR"
     gene            4606..5046
                     /locus_tag="VFY74_11185"
     CDS             4606..5046
                     /locus_tag="VFY74_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5932292.1"
                     /translation="MKPSLRAGLKHSFSYLVPEAKTVPHLYRESAELRAMPEVFATGF
                     MVGLMEWTCVELLAPHLDEGEGSLGTHIDVSHKAATPVGFTVTVEAECIELRGPRARF
                     KIVAHDGVDEIGAGTHERFIVAWDRFNRSVAAKRAKAAGTKVDA"
     gene            5143..5493
                     /locus_tag="VFY74_11190"
     CDS             5143..5493
                     /locus_tag="VFY74_11190"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5932293.1"
                     /translation="MAICHCGMCRRWSGGVFMAVDCSAGFEIEDDSSVGLFQSSPWGE
                     RGFCKSCGSTLFWRMRDRSSVHVSAQAFDHPEQFKLTLEIFIDEKPTNFAFANATQKM
                     TGAEAMTVSKAEQE"
     gene            5498..6724
                     /locus_tag="VFY74_11195"
     CDS             5498..6724
                     /locus_tag="VFY74_11195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5932294.1"
                     /translation="MTETETPAPDADSGKREGDDRKFTINFGPQHPAAHGVLRLVLEL
                     DGEVVERVDPHIGLLHRGTEKLIEYKTYLQALPYFDRLDYVAPMNQEHAYALAVEKLV
                     DVTVPKRARLIRVLYSEIGRLLSHLLNITTQALDVGALTPPLWGFEEREKLMIFYERA
                     SGSRMHAAYFRPGGVHQDLPPELVADIYAFCDPFLKVCDDIEDLLTENRIFKQRNVDI
                     GVVKLDDAWERGFSGVMVRGSGAAWDLRKNQPYECYEELAFDIPIGKNGDCYDRYLVR
                     MEEMRQSVRIMKQCCERLTTEHGPVTVENHKMVPPSRAVMKSSMEALIHHFKLYTEGY
                     HVPEGQVYAAVEAPKGEFGVYLVSDGTNRPYRCKIRAPSYPHLAAMDFLCRGHMLADV
                     SAVLGSIDVVFGEIDR"
     gene            6736..7200
                     /locus_tag="VFY74_11200"
     CDS             6736..7200
                     /locus_tag="VFY74_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932295.1"
                     /translation="MARTALTLAAVAMLSFAAPQAGGAGQSGPAPAPPAAPPEPAPAQ
                     KTGEGAGTKTEADDKPKPAETAPSPGAQSPAPPSDGDSQSTLAALMREGFEVRTTAFV
                     PAEAVTRQSGKVSSDAIVVTLQKEAASAVCFYTLKAYVSGKLSTIPACIVHR"
     gene            7259..8002
                     /gene="nuoE"
                     /locus_tag="VFY74_11205"
     CDS             7259..8002
                     /gene="nuoE"
                     /locus_tag="VFY74_11205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX5932296.1"
                     /translation="MSVRRLAADQPEGFAFTPENLEWARAEMKKYPEGRQASAVLSIL
                     WRAQEQMGGWLPEPALRYVADLLGLPYIRLYEIATFYTMFNLAPVGRHFVQVCGTTPC
                     WLRGADEIKDTCRRLIGAPGHVTPDGLFSWIEVECLGACVNAPMAQIDKDYYEDLTPE
                     SLEDILESLKAGRPVTPGSQIGRQTSAPLGGPTTLTDPDLYPEHETLEPASPALTDAE
                     AKRPGAAANLRERPVQKPPIAKDRKPRQK"
     gene            8002..9303
                     /gene="nuoF"
                     /locus_tag="VFY74_11210"
     CDS             8002..9303
                     /gene="nuoF"
                     /locus_tag="VFY74_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5932297.1"
                     /translation="MLEDKDRIFKNLYGLHDWRLVAARARGAWADTKTIIERGHEAII
                     DEVKASGLRGRGGAGFPTGLKWSFMPTKFDGRPSYLVVNADESEPGTCKDRDIMRHDP
                     QLLIEGAFLSSFAMRAHVSYIYIRGEFIREREILQTAIDEAYDAKLIGKNNVHGWDFD
                     LYLSHGAGAYICGEETALLESLEGKKGQPRLKPPFPANVGLYGAPTTVNNVETIAVVP
                     DILRRGATWFASLGPPNNTGTKIFCISGHVNTPCNVEEEMGIPLRELIDRHAGGVRGG
                     WDNLLAVIPGGSSVPCIPANQCDDLPMDFDSLKALKTGLGTAGVIVMDKSTDIVRAIA
                     RISYFYKHESCGQCTPCREGTGWMWRVLERMARGEAEKDEISMLLDVSYQVEGHTICA
                     LGDAAAWPVQGLIRHFRHVIEERIDARATKPIAPSVPVAAE"
     gene            9310..9789
                     /locus_tag="VFY74_11215"
     CDS             9310..9789
                     /locus_tag="VFY74_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932298.1"
                     /translation="MVWFWDNAVTIGTLVMAAAAIVALIYAHWQISENRRAERRGNAN
                     ELWRETLRLGFDNPKLSDPTANLAAFDYENLTIDGSPELFQKYEMFVDTILNASEEIF
                     SVSPTKEWLAALRLQLRPHRAYLQSQHYVDSGYIEQYTPKFRAFLHATLSGRPERHA"
     gene            9782..11896
                     /gene="nuoG"
                     /locus_tag="VFY74_11220"
     CDS             9782..11896
                     /gene="nuoG"
                     /locus_tag="VFY74_11220"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019565476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX5932299.1"
                     /translation="MPRLIIDGKEHDVPADITLIQACALAGIEIPRFCYHERLSIAGN
                     CRMCLVEVVGMPKPMASCALAVNDLRPAPDGTLPTINTNTETAKKAREGVLEFLLINH
                     PLDCPICDQGGECDLQDQAMAYGFDAGRFQDNKRAVEDKYLGPLIDTYMTRCIHCTRC
                     VRFMTEVAGVPELGAIGRGENMEITTYLERGLLSNLSGNVVDLCPVGALTSKPYAFTA
                     RPWELTKTPSIDTMDALGSAIRVDSRGREVMRILPRDNDAINEEWISDKTRHVWDGLR
                     TQRLDRPYVRREGKLVPASWDEAFKVIAERLRGLDGSRFAAIAGDLAAAEEMFALKLL
                     ADALGSPNIDCRQDGAKLEPGLGRATYLFNTTIAGIDEADAFLLVGTDPRAEAPVLNA
                     RILKRSRRALGKLPIGMIGREADLTYKYEYLGAGPETLAELASGAHPFLQKLEAAERP
                     MLILGQGALARPDGAEVLALAAKAAIAIGAIKPTLAWNGFNVLHAAASRVGGLDLGFV
                     PGEKGLDVDSMLEAAAGGTVEVVYLLGADEIDMERLGRAFVIYQGSHGDAGAHRADVI
                     LPGAVYTEKAGIYVNTEGRPQITARAVFPPGDAREDWKIVRALSEVLGKTLPFDSVQE
                     LRAKLFARHPHLAELDSIVPADAAAIEQLAGRPAKPGKDRFEPAITDFYLTNPIARAS
                     GIMASLSALHAGHDDKATGTDG"
     gene            11889..12911
                     /gene="nuoH"
                     /locus_tag="VFY74_11225"
     CDS             11889..12911
                     /gene="nuoH"
                     /locus_tag="VFY74_11225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX5932300.1"
                     /translation="MAEFWSSYGLPFALILAQSMALLVVLLLIVAFLLYADRKVWAAV
                     QMRRGPNVVGPWGLLQSFADLLKFVFKEPIIPAGANKGIFLLAPLITAGLALSAWAVI
                     PVAKGWEVANLNVGILYVFAISSLQVYGVIMAGWASNSKYAFLAALRSAAQMVSYEVS
                     IGFVIITVLLCAGSLNLGEIVEAQNGALGLFGWYWLPLLPMFVVFFISALAETNRPPF
                     DLLEAESELVAGFMVEYSSTPYMLFMLGEYVAIVLMCALGTVLFLGGWLPPFPVAPFT
                     WIPGVVWFILKACLLFFLIAMVKAMVPRYRYDQLMRLGWKVFLPLSLAMVMIVAAILK
                     LIEQAA"
     gene            12925..13416
                     /gene="nuoI"
                     /locus_tag="VFY74_11230"
     CDS             12925..13416
                     /gene="nuoI"
                     /locus_tag="VFY74_11230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX5932301.1"
                     /translation="MKRWAESAKSLLLIEFLSAFWLALKYFVAPKATVNYPFEKGPLS
                     PRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRDDGTRRTTRYDIDMVK
                     CIYCGFCQEACPVDAIVEGPNFEFATETREELYYDKERLLANGDRWEREIAKNLELDA
                     PYR"
     gene            13498..14106
                     /locus_tag="VFY74_11235"
     CDS             13498..14106
                     /locus_tag="VFY74_11235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5932302.1"
                     /translation="MLGALFFYLFATILVGAGFMVIAAKNPVHAVLFLILAFFNAAGL
                     FVLLGAEFLAMILVVVYVGAVMVLFLFVVMMLDVDFAQLRAGFITYLPVGATIGLLLL
                     VELILILGAWVTAPEMTAQAPTPPAADIGNTEALGLILYTRYVYLFEAAGLILLVAMI
                     GAIVLTLHHKRDVKRQKVGLQVRRTRDESVELVDVESGRGLS"
     gene            14103..14411
                     /gene="nuoK"
                     /locus_tag="VFY74_11240"
     CDS             14103..14411
                     /gene="nuoK"
                     /locus_tag="VFY74_11240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX5932303.1"
                     /translation="MTVGLSHYLTVAAILFTLGIFGIFLNRKNVIVILMSVELMLLAV
                     NLNLVAFSAFHLDLVGQIFAMIVLTVAAAEAAIGLAIVVVYFRNRGTIDVAEIDLLKG
                     "
     gene            14424..16343
                     /gene="nuoL"
                     /locus_tag="VFY74_11245"
     CDS             14424..16343
                     /gene="nuoL"
                     /locus_tag="VFY74_11245"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5932304.1"
                     /translation="MYQAILFLPLVGAIFAGFLGPLAGARASEVVTTTLVLITALLSW
                     LAFYEVALQGQEQRIELARFIVSGDLKSIWSLRIDTLTAVMLVVVSTVSGLVHLYSIG
                     YMEEDDGRPRFFAYLSLFTFAMLALVTADDFVQLFFGWEGVGLASYLLIGFWYHKPSA
                     NAAAIKAFIVNRVGDFGFALGIFAVFALFDSVQYDAVFASAPTHVGEKVNFLWMQVDA
                     LTLICLLLFMGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPL
                     FELAPIALAVVTIVGATTAFFAATVGLVQNDIKRVVAYSTCSQLGYMFAAEGVGAYGV
                     GMFHLFTHASFKALLFLGAGAVIHALHHEQDLRRMGALRRVLPFTWAMMLIGTLSLTG
                     FPFTAGFVSKDAIIESTYAAHSWVGMYAFVLTVVAAVLTSFYSWRLMFLAFEGKPREP
                     KELLEHAHEPNLTMTVPLAVLALGALLTGGLFAHLFIGTEQAEFWRGSLVVHEHHHVP
                     LWVVLAPSVAMALGFAVAWYFYITNPLVPFGLAKRFRGAYQFLLNAWYFDALYDALFV
                     RPALRLGRFLWKTGDGTIIDGIGPDGVAARVIDIANRAIRLQTGYIYHYAFVMLIGVA
                     LLITYFLFAGGGLNP"
BASE COUNT         2889 a         5385 c         4952 g         3215 t
ORIGIN      
        1 agaaatggcc tccaccatgg gtttcatgtg ccgcgccttg acttgacgga gtggcgcatt
       61 taagtccgca ccactcctga aaagggggcg tagctcagtt ggttagagcg ccggcctgtc
      121 acgccggagg ccgcgggttc gagtcccgtc actcccgcca gcatctcgct tgaacggccc
      181 ttccggcacc tcagctttag agaccgttag ccccattccg atagcgtgcg cgaccttggg
      241 tcagccagtc gccgcccgtc gcaaaacatt tgggaggttg ccttgatcgg tacgtcgtcg
      301 aagcttcttg cttgcgcttt ggccctggcg ctcagcgcaa gctcttacca atggtcccac
      361 acctgcaccc ggatcctttg cgttggcgac gatcgccgga cggtcgatgg actggaacga
      421 cgacacgcgg accaatcttt gggtctttcc ccgcggcatg cagcgcgacg gtgccgccgg
      481 ccccaattcg gcgaaatgga cctcaaaata cggcagcctc ggcacggcgg tctatgacgc
      541 cgccgtcgcc gacggcatga acgagaaagg tctcgtcgcc agtctgctct accttgccga
      601 tgccgactac ggcaaagccg acggcaagcc catgctttcc atcagcgctt ggccgcaata
      661 cgtgctcgac aatttcgcca ccgtcgccga ggcggtcgag acgttgcgga cggagccgtt
      721 ccgcctcggg gcgccggcca tgcccactgg cgacgcgggc tcggtgcatc tcgcgatttc
      781 ggacccgaca ggcgactccg ccatcttcga gtatatcggc ggcaagctcg tcatccatca
      841 cggcaggcaa tatcaggtga tgaccaacga gccgcccttc gatcagcagc tcgcgctcaa
      901 cgcctattgg caggagatag gcggagcgac attcctcccg ggcaccatcc gccccgccga
      961 ccgcttcgtc cgcgcctact atttggtcac cgcgatcccc aagagcaccg tcccgggcat
     1021 catcgcagcc gtgcccgacg gcacctatcg caatcaggcc gtggcgagcg tcgcgagcgt
     1081 cctgcgcgcg gtcagcgtgc cattcggcat gtcttcgccg ggagagccca atgtcgcctc
     1141 gaccctgtgg cgcacctggg ctgaccagac caacctcgtc tacttcttcg attcggccac
     1201 gagccccaat acgttctggg tgccgctcgc cgatctcgac ttcggcgagg gagcgccggt
     1261 gaagaagctt acgctcaccg gcggcaaggt ctattcgggc aatacggccg acaagttcga
     1321 gccggcgaag cccttcgcct ttatgcccta cgcgtcgtga gccagcaggg ttcactccaa
     1381 ggggtcgcat tccccttcac gcaccgatgt ttaagacgtc cgcttggggc gcgaaagtcg
     1441 acgcttgttg gtgcggacat gacctcgata acgtcgcaat accttatgca gtgacccgcc
     1501 agtggcgagc gtgccgagcg cctccgcgag ggcaaagccc ttctctgtta ccatgagctg
     1561 cgcctcagtt cggctccggc ttccgccctg agcaagcttc ttcaggcgaa ggctcatcac
     1621 ctcctgtgcc tcgtgacaaa gacgcgcgga agccagggca aaatccatcc aaattttgat
     1681 catggctctc ccatacgaga agaccggcgt ccgaaagcgg aaccggacaa aggatcggaa
     1741 agagcggaga ttagcggcgt gttcttgaga gcacaccaac caggaaacct agcccaaggg
     1801 caaaggccac cgccatgagc ggattgcgct tcatcgccgc gtgcgcatcg tcaaaagcct
     1861 cgacggccat gtcttgggcg cggccgtatt gtttggatgc catggcggca acatcgccgg
     1921 cgacatcggc gatctcctcg cccatggacc tggcgccctc agccgcggtc ttgacggact
     1981 ctgcagcccg acgaccggct ggtgagttgg cggcgtctct gtaagcttgg ctcccggtgg
     2041 tcatcccatg atccttatat tgttcttggc cagccgaaag cggcaccaag ttggatatga
     2101 gtgccaactt gcgacgcaga ggaacgttcc gtcgctgcgc gaattagcat cataggtcct
     2161 cgtcccgtcc cagcgccgag gacaatcccc cttaggccgc agaggcagta ccatgtcaaa
     2221 gtcgtccaat tctaaagttg ccgcgaagaa ggcgcgcggt gcccgtggca agaccgtcgc
     2281 caaagtcgct cgcgaaaagg cgcggaccac acgcgcttct caccggagca agccgaaagt
     2341 tgtcaccgag gcggcgcacg taccgctcag gaccgctccg gaaaaagcca tcgagcaggc
     2401 gcgatacaga tcgcagcatc gctgctcttt tgaggcattt cgcgacagcc aagttaaagg
     2461 cacgataaat gccatcgctg aaaggagttt ggctcagacg cgcgaggcct atgagcgctc
     2521 caaacacact cttcaagccg tgctcgcgag ctgggaaaga tctttcggtg ccgccggtca
     2581 gggcgctata gcgctcaatc gtaaggtcat cgaggttgcc gaccgcaacg ttaataacgc
     2641 atttgatctt gcggctaacc ttgccgcggc gaagaacttt gctgacgtga tggcgtcgca
     2701 cgcaacctat tggcgcaaac aattcggcaa tctcagcgct cgaagctgag gacgtgcgca
     2761 ccaaagcggc cgcgacggca gaactgatat cccggcatgg gctggatcgt gggcggtcct
     2821 ccaattgcgc gacagtggga taggtcgccc aacaccgaag ctgatcgaag gcgtgtggcg
     2881 gttcatcggc tgggctattt gtaagcacgc cgaaaagccc tatattgcca aagagccccg
     2941 ttagggcagt tggtgacctt aacccattga tttgcttggt ttgggtgagt ccgtacacac
     3001 ccgccatcta caggcctcct ccaacgccga aagaggacta tggaccagct gctccaggac
     3061 tatctgccgc tcgtcatctt catcggcatc gcctttgcgt tcagcgcggc gcttttgctt
     3121 gcgcccttcg ccgttgccgt caggaagccc gatccggaga aggtctcggc ctatgaatgc
     3181 ggcttcgacg ccttcggcga cgcgcgcatg aaattcgacg tccgcttcta ccttgtgtcg
     3241 ctcctgttca ttatattcga tctcgaggtg gcatttctgt tcccctgggc cgtggccttc
     3301 gacgcgatcg gcgccttcgg cttctgggca atgatgatct tcctcgccgt tctcaccatc
     3361 ggcttcatct atgagtggcg gaagggcgcg ctcgaatggg attgacgctt gagccgaaat
     3421 tcgacaagga cggacggagc ggcggcattg tcacgcccgg cgcgacgagc ctcggcacct
     3481 tctcggagga gctcgccgac aagggattcc tgctcacgac cgcagacgat ctcatcaact
     3541 ggtcgcgcac cggctcgctg atgtggatga ccttcgggct tgcctgctgc gcgatcgaga
     3601 tgatgcaggc gtccatgccg cgttacgatc tcgagcgttt cggctttgcg ccccgcgcca
     3661 gtccgcgcca atccgacgtg atgatcgtgg ccggcacgct gtgcaacaag atggcgccgg
     3721 cgctgcgcaa ggtctatgac cagatgcccg agccgcgcta tgtcatctcg atgggatcct
     3781 gcgccaatgg cggcggctac tatcactact cctactccgt ggtgcgcggc tgcgaccgta
     3841 tcgttccggt cgacatttac gtgcccggct gcccgccgac ggccgaagcg ctcgtttatg
     3901 gcgttctgct tctacaaaag aagatcagac ggaccggcac catcgagcgc tgatcggacg
     3961 attgcctttt cctggagccg cgatggccga cgcccttgac aagctcgccg cctatctccg
     4021 cgaccgcctc ggaccaaaac tttcgagtgc ggaacttgcg ctcggcgagc tcaccatcac
     4081 ggtcgaacgt gaccagatcc ccgaggtcat ccgcttcttg cgcgacgact cccactgccg
     4141 cttcggctct ctgatcgaca tttgcggcgt cgactatccg gagcgcgagg agcgcttcga
     4201 cgtcgtctat catttcttga gcccatggct gaaccaccgc atccgcgtcc gcaccatgac
     4261 gaatgcggca acgccggtac cgagcattgt aagtctgttc ccgggggccg attggttcga
     4321 gcgcgaggcc tacgaccttt acggcatctt gttctcgggc caccctgacc tcaggcggct
     4381 cctcaccgac tatggcttcc agggtcaccc tttgcgcaag gacttcccgc ttaccggctt
     4441 cgtcgaactc cgctacgacg atacgcagaa gcgcgtggtc tacgagccgg tgaagctgat
     4501 gcaggaatat cgcagcttcg atttcctgag cccatgggag ggaatggaca acgccatccc
     4561 cggcgacgag aaggccggcg aagcaccccc tcgggagggc aagcgatgaa gccgtcgttg
     4621 cgagccgggc tgaagcacag cttctcctac ctcgtgcctg aggccaagac cgtgccgcat
     4681 ctctatcgcg agtcggccga gcttcgggcg atgccggaag tgttcgccac gggcttcatg
     4741 gtcggactga tggaatggac gtgcgtagag ctgctcgcgc cgcatctcga cgaaggcgag
     4801 ggaagcctcg gcacgcatat cgacgtgtcg cacaaggcgg cgacgccggt cgggttcacc
     4861 gtcaccgtcg aggcggaatg tatcgagttg cgggggccgc gggcgcgctt caagatcgtc
     4921 gcccatgacg gcgtggatga gatcggcgcc ggcacacatg agcggttcat cgtggcgtgg
     4981 gatcgattca atcgcagcgt tgccgccaag cgcgcgaaag ccgccggcac caaggtggac
     5041 gcctaacaga gttaggagcc aggagtgacc tcgtcgaata catgctccgg acgctgcctc
     5101 tgtggcgcgg tacgattcac tgccaggccg gagaaggccg agatggccat ttgccattgc
     5161 ggcatgtgcc gccgctggtc cggcggagtg ttcatggcag tcgattgcag cgcgggtttt
     5221 gagatcgagg acgatagttc cgtcggactt tttcagtctt cgccttgggg tgaacgcggc
     5281 ttctgcaaat cttgcgggag caccttgttt tggcgcatgc gggatcgctc ttccgttcat
     5341 gtttccgcgc aggccttcga tcatcccgag cagttcaagt tgacgctcga aatcttcatt
     5401 gacgagaagc ccacgaattt cgccttcgcc aacgcaacgc agaagatgac cggcgccgag
     5461 gccatgaccg tgtcgaaggc ggagcaggaa tgacgagatg accgagactg aaacgccggc
     5521 gcccgacgcc gacagcggca agagggaagg ggacgaccgc aagttcacca tcaatttcgg
     5581 tccgcagcat ccggccgccc acggagttct taggctcgtg ctcgagctcg acggcgaggt
     5641 ggtcgagcgc gtcgatccgc atatcgggct gctccaccgc ggcaccgaga agctgatcga
     5701 gtacaagacc tacctacagg cgcttcccta tttcgaccgg ctcgactacg tggcgccgat
     5761 gaatcaggag cacgcctacg cgctcgcggt cgagaagctt gtcgacgtca ccgtgccgaa
     5821 acgcgcacgg ctcatccgcg tcctttattc ggagatcggg cggctcctgt cccatctcct
     5881 caacatcacc acgcaagccc tcgatgtcgg cgcgttgacg ccgccgctct ggggcttcga
     5941 ggaacgcgag aagctgatga tattctacga gcgggcctcg ggcagccgca tgcacgcggc
     6001 ctatttccgc ccgggcggcg tgcatcagga tcttccgccg gaactcgtcg ccgacatcta
     6061 cgccttctgc gatcctttcc tcaaagtctg cgacgatatc gaagaccttc tcaccgagaa
     6121 ccgcatcttc aagcagcgca atgtcgatat cggcgtggtg aagctcgacg acgcgtggga
     6181 gcgcggtttc tccggcgtca tggtcagggg ctcgggcgcc gcctgggatt tgcgcaagaa
     6241 ccagccttac gaatgttacg aggaactcgc cttcgatatc ccgatcggca agaacggcga
     6301 ttgctacgac cgctatctcg ttcgcatgga ggagatgcgg caatcggtcc gcatcatgaa
     6361 gcaatgctgc gaacggctca ccacggagca cggaccggtc acggtcgaaa accacaagat
     6421 ggtgccgcca tcgcgcgccg tgatgaagag ttcgatggaa gcgctcattc accacttcaa
     6481 gctctatacc gagggctacc acgtgcctga gggccaagtt tacgccgcgg tcgaagcgcc
     6541 caagggggag ttcggcgtct atctcgtctc ggacggcacc aaccggccct accgctgcaa
     6601 gatcagggca cccagctatc cgcatcttgc cgccatggac ttcctctgtc gcggccatat
     6661 gctcgccgac gtgtcggccg tgctcggctc cattgacgtc gtgttcgggg aaatcgaccg
     6721 gtgagggaaa accgcatggc gagaacggcg cttaccctcg ccgccgtcgc aatgctcagc
     6781 tttgccgcgc cacaggcagg gggcgcggga cagagcgggc cggcgccagc tccaccggcg
     6841 gctccgcccg aacctgcacc ggctcaaaag acgggtgagg gcgcgggcac gaagaccgaa
     6901 gcagacgata agccaaagcc cgccgagacc gcgcccagcc caggcgcgca gagccccgcg
     6961 cctccgtcgg acggcgacag ccaatccacg ctcgccgcgc tgatgcggga aggattcgag
     7021 gtccgcacca cggccttcgt tccggccgag gcggtgacac gccaatcggg caaggtctcc
     7081 tccgacgcca tcgtcgtgac cctgcaaaag gaggccgctt ccgccgtttg cttttatacg
     7141 ctgaaggcct atgtgagtgg gaaactgagt acgattcccg cctgtatcgt tcaccgctag
     7201 gacgcacgcc cagcagcttc gcccagcccg gaatgagaga gaagaacacc ttaagccaat
     7261 gagcgttcga cgcctcgccg ccgatcagcc tgagggcttc gccttcacgc cggagaatct
     7321 cgaatgggcg cgcgcagaga tgaaaaaata tccggaggga cggcaggcct cggccgtcct
     7381 gtccatcctt tggcgcgcgc aggagcagat gggaggctgg ctgcctgagc ccgcgctccg
     7441 ctacgtcgcc gatctgctcg gcctccctta catccggctc tacgagatcg cgaccttcta
     7501 caccatgttc aatctcgctc cggtcggccg gcacttcgtg caagtgtgcg gcaccacgcc
     7561 ttgctggctt cggggggccg acgagatcaa ggatacctgc cggcgattga tcggcgcgcc
     7621 gggacatgtg acccctgacg ggctcttctc ctggatcgag gtcgagtgtc tcggcgcctg
     7681 cgtcaacgcg ccgatggcgc agatcgacaa ggactattac gaagatctca cgccggagag
     7741 cctggaagac atcctcgaga gtttgaaggc ggggcgcccc gtgaccccag gctcgcaaat
     7801 aggacgacag acctctgcgc cgcttggagg cccgaccacg ctcaccgatc ccgacctcta
     7861 cccggaacac gagactctgg agccggccag tcccgcactg accgacgcgg aggcgaaaag
     7921 gccaggtgcc gctgcaaatc ttcgtgagcg gccggttcaa aagccgccga tcgccaagga
     7981 ccgcaagccg agacaaaagt gatgctcgaa gacaaggatc gcatcttcaa aaatctctac
     8041 ggcctccatg actggcggct ggtggctgcg cgcgcgcgcg gcgcgtgggc ggacacgaag
     8101 acgatcatcg agcgcggtca tgaggcgatc atcgacgagg tgaaggcctc gggcttgcgc
     8161 ggccgcggcg gcgccggttt cccgactggc ctgaaatggt cgttcatgcc gaccaagttc
     8221 gacggccggc cctcctacct cgtggtgaat gccgatgagt ccgagcccgg cacctgcaag
     8281 gaccgggaca tcatgcggca tgacccgcag cttctcatcg aaggcgcgtt cctatcgtcc
     8341 ttcgccatgc gcgctcatgt ctcctatatc tacatccgcg gcgagttcat ccgcgagcgc
     8401 gagatcctgc aaacggccat cgacgaggcc tacgacgcga agctgatcgg caagaacaat
     8461 gtccatggct gggatttcga tctctatctc agccacgggg caggggcgta tatttgcggc
     8521 gaagagacgg cgctgctcga aagcctcgaa ggcaagaagg gccagccgcg cctgaagccg
     8581 ccgttcccgg caaatgtcgg cctttacggc gcgcccacca ccgtcaacaa tgtcgagacc
     8641 atcgccgtgg tccccgatat cctgcgccgt ggcgccacgt ggttcgcctc gctcggcccg
     8701 ccgaacaaca ccggcaccaa gatcttctgc atctccggac acgtgaacac cccatgcaac
     8761 gtcgaggagg aaatgggcat accgttgcgc gaactgatcg atcgccatgc cggaggcgtg
     8821 cgcggcggct gggacaatct tttggccgtc atccccggcg ggtcatccgt tccttgcatt
     8881 ccggcgaacc agtgcgacga tctcccgatg gatttcgaca gcctcaaggc gctgaagacg
     8941 gggctcggca ccgcgggcgt catcgtcatg gacaaatcga ccgacatcgt ccgcgccatc
     9001 gcccgcatct cctatttcta taagcacgag tcctgcggcc agtgcacgcc ctgccgcgaa
     9061 gggacgggct ggatgtggcg agtgctcgag cgcatggcca gaggcgaggc cgagaaggac
     9121 gagatcagca tgctgctcga tgtgagctac caggtcgaag gccacaccat ttgcgcgctc
     9181 ggcgacgcgg ccgcctggcc ggtccagggc ttgatccgcc attttcgcca tgtgatcgag
     9241 gagcgtatcg acgcgcgtgc gacgaagccg attgcgccct ccgttccggt ggctgctgag
     9301 tagggccgcg tggtgtggtt ctgggacaac gccgtaacga tcgggaccct ggtgatggcc
     9361 gccgctgcca tcgtcgccct gatctatgcg cattggcaga tctccgagaa ccgcagggcc
     9421 gaacgccggg gcaacgccaa tgagctttgg agagagacgc tccgactggg tttcgacaat
     9481 cccaagctct cagatccgac ggcgaacctc gccgcgttcg actacgagaa tttgaccatc
     9541 gacggcagcc ccgagctgtt ccagaaatac gagatgttcg tggacacgat cctgaatgca
     9601 agcgaggaga ttttcagtgt ctcgccgacc aaggagtggc tcgcggcgct tcgtctccag
     9661 ctccgtccgc atcgcgccta tctgcaatcg cagcactacg tggattccgg atacatcgag
     9721 caatacaccc cgaaatttcg cgcgttcctg cacgccacgt tgagcgggag gccggaacgc
     9781 catgcctagg ctcatcatcg acggcaagga gcacgatgtt cccgcagaca tcaccctgat
     9841 ccaagcgtgc gcgctcgcag ggatcgagat tccgcgcttc tgctatcacg agcgcctgtc
     9901 catcgccggc aattgccgca tgtgcctggt cgaagtggtc ggcatgccga aacccatggc
     9961 ctcttgcgca ctcgccgtca acgatctcag gcccgcgccc gacggcacgc tgccaaccat
    10021 caacaccaat accgagacgg cgaagaaggc ccgcgagggc gtgctcgagt tcctgctcat
    10081 caatcacccg ctcgactgcc cgatctgcga ccagggcggc gaatgcgatc ttcaagacca
    10141 ggccatggcc tacggcttcg acgccgggcg cttccaagac aacaagcgcg cggtcgagga
    10201 caagtatctc ggaccgctga tcgacactta catgacgcgc tgcatccact gcacgcgctg
    10261 tgtgcgattt atgaccgagg tggcgggcgt gcccgagctc ggcgccattg ggcgcggcga
    10321 gaatatggag atcacaacct atctggagcg cgggctgctc tcgaacttgt cgggcaacgt
    10381 ggtggatctc tgccccgttg gcgctctcac ctcgaagcct tacgctttca cggcgcgccc
    10441 ctgggagctc accaagacgc cgtcgatcga tacgatggat gcgctcggca gcgccatcag
    10501 ggtcgattcc cgcggccgcg aggtcatgcg catcctgccg cgcgacaacg atgctatcaa
    10561 cgaggagtgg atctccgaca agacccgcca tgtgtgggac ggcttgcgca cccagcggct
    10621 cgaccggcct tacgtccgtc gcgagggcaa gcttgtgccg gcaagttggg acgaagcctt
    10681 caaggtcatc gccgagcggc tgcgtgggct cgacggctcg cgcttcgccg cgattgcggg
    10741 cgacctcgcc gcggcggaag agatgttcgc gctcaagctc ctcgccgatg cgctcggctc
    10801 tcccaacatc gattgccgcc aggacggcgc caagctcgaa cctggcctcg gccgcgcgac
    10861 gtatctcttc aatacgacca tcgccggcat cgacgaggcc gatgccttcc ttctcgtcgg
    10921 caccgacccg cgggcagaag cccccgtgct caacgcgcgc atcctcaaac gctcgcggcg
    10981 cgcgctgggg aagctgccca tcgggatgat cggccgagag gcggacctga cctacaagta
    11041 tgagtatctc ggcgccggtc ctgagacgct tgccgagctt gcgagcggcg cgcatccgtt
    11101 cttgcagaag ctcgaagcag ccgagcgccc gatgctgatt ctgggacagg gcgcgctggc
    11161 aagacccgac ggcgccgagg tcctcgccct tgccgccaag gccgcgattg ccatcggcgc
    11221 aatcaagcct acgctcgcct ggaacgggtt caacgtgctg cacgcggcgg cctcgcgcgt
    11281 tggcggcctc gaccttggtt tcgtccccgg cgaaaagggt ctcgatgtcg attccatgct
    11341 cgaagccgcc gccggcggca cagtcgaggt ggtctatctc ctcggcgccg acgagatcga
    11401 catggagcga ctgggacgcg ccttcgtcat ctatcagggc agccacgggg acgccggggc
    11461 tcaccgcgcc gacgtcattc tccccggcgc cgtctatacc gagaaggccg gcatttacgt
    11521 caacaccgaa ggccggccgc agatcacggc gcgtgccgtc tttccgccgg gggacgcgcg
    11581 cgaggactgg aagatcgtga gagccttgtc ggaggttctc ggcaagacgc tgcccttcga
    11641 cagcgtgcag gaacttcgcg ccaaattgtt cgcgcgccat ccgcatttgg cggagctcga
    11701 ttccatcgtt cccgctgatg cggccgcgat cgagcagctc gccggacggc cggccaagcc
    11761 cggcaaggat cgcttcgagc ccgccatcac ggacttctac ctgaccaatc cgatcgcccg
    11821 ggcgtcaggg atcatggcga gcttgagcgc gcttcacgcc ggccatgacg acaaggcgac
    11881 aggcaccgat ggctgagttc tggtcgagct atggtttgcc gtttgcgctg atcttggcgc
    11941 aaagcatggc ccttctcgtc gtccttctcc tgatcgtggc cttcctgctc tacgccgacc
    12001 gcaaagtgtg ggccgcggtg cagatgcggc gcgggccgaa tgtcgtcggc ccctgggggc
    12061 tgcttcaatc cttcgccgac cttctcaaat tcgtgttcaa ggaaccgatc attccggccg
    12121 gcgccaacaa gggcatcttc ctgcttgcgc ctttgatcac ggcagggctc gcgctctcgg
    12181 cttgggcggt cattcccgtc gccaagggtt gggaagtcgc caatctcaat gtcggaattc
    12241 tctacgtctt cgccatctcg agcctgcaag tctacggcgt gatcatggcg ggctgggcgt
    12301 cgaactcgaa atacgccttc ctcgccgcgc tccgctcggc ggcgcagatg gtctcctacg
    12361 aggtttcgat cggtttcgtc atcatcaccg tgctcctgtg cgcgggctcc ctcaatctcg
    12421 gcgagatcgt cgaggcgcaa aacggagcct tgggcctgtt cggatggtac tggctgccgc
    12481 ttctgcccat gttcgtggtg ttcttcattt ccgctctcgc cgagacgaac cgcccgccct
    12541 tcgatctcct tgaagcggag tctgagctgg tggcggggtt catggtcgaa tactcgtcca
    12601 cgccctatat gctgttcatg ctgggcgagt atgtcgccat cgtgctcatg tgcgcgctcg
    12661 gcacggtgct ctttcttggc ggctggctgc cgccctttcc ggtggcgccc ttcacctgga
    12721 ttccgggcgt ggtatggttc attttgaagg cctgcttgtt gttcttcttg atcgccatgg
    12781 tcaaagccat ggtgccacgc taccgctacg atcagctgat gcggcttggc tggaaggttt
    12841 ttctgcctct gtctctcgcc atggtcatga tcgtcgcggc gatcctgaag ctgattgagc
    12901 aggccgcatg aaaggaccgg acgcgtgaag cgctgggcgg aatccgctaa gtcactgtta
    12961 ctgatagagt ttctgtcagc cttttggctc gccctgaaat acttcgtggc gcccaaggcg
    13021 acggtcaact acccgttcga aaagggtccg ctctccccgc gcttccgcgg cgagcatgcg
    13081 cttcgccgct atccgaacgg cgaggagcgc tgcatcgcct gcaagctttg cgaggccatc
    13141 tgtccggccc aggccatcac catcgaggcc ggtccgcggc gcgacgacgg cacccgccgc
    13201 accacccgct acgacatcga catggtgaaa tgcatctatt gcggcttctg ccaggaagcg
    13261 tgcccggtcg atgcgattgt ggaaggaccc aatttcgaat tcgccaccga gacccgggaa
    13321 gagctttact acgacaagga gcggctcttg gcgaatggcg accgctggga gcgcgagatc
    13381 gccaagaacc tcgagcttga cgcgccatac cggtgagggg aattccgcgc atgcgagccg
    13441 cagtcgacgc cacctcttcc cgatggggag aggcctctcc gagatcgtaa ccctccgatg
    13501 ctgggcgcgc tcttcttcta tttgttcgca acgatattgg tcggcgcggg cttcatggtc
    13561 atcgccgcga agaaccccgt gcacgcggtg cttttcctta tcctggcctt cttcaacgcc
    13621 gccggtctct tcgtgctgct gggcgccgag ttcctggcga tgatcctggt cgtggtctat
    13681 gtcggcgcgg tcatggtgct gttcctattc gtggtgatga tgctcgatgt cgattttgct
    13741 cagctccgcg caggcttcat cacatatctg ccggtcggcg ccaccatcgg cctcctgctt
    13801 ctcgtcgagc tgatcctgat cctcggcgcc tgggtcacgg cacccgagat gacggcgcag
    13861 gcgccgacgc ctcccgccgc cgatatcggc aacaccgagg cgctcggtct cattctctat
    13921 acgcgatacg tctatctgtt cgaggcggcc ggtctcatcc ttctcgtcgc catgatcggc
    13981 gccatcgtgc tgacgctgca tcacaagcgg gacgtgaagc gccagaaggt cggcctccag
    14041 gtgcggcgca cgcgggatga gtcggtcgag ctcgtcgatg tggaatcggg gaggggcctg
    14101 tcatgaccgt cggcctctcc cattatctca ccgtcgccgc catcctgttc acgctcggca
    14161 ttttcggcat cttcctcaat cgcaagaatg tcatcgtcat tctgatgtcc gtcgagctga
    14221 tgctgctcgc cgtgaacctc aacctcgtcg ccttctccgc cttccactta gacctcgtgg
    14281 ggcaaatctt cgccatgatc gtgcttacgg tggcggcggc cgaggcagcc atcggtctcg
    14341 ccatcgtggt cgtctatttc cgcaatcgcg gcacgatcga cgtcgccgaa atcgacctgc
    14401 tgaagggctg agccgcaaga ccgatgtatc aggcgatcct cttccttccc ctcgtcggtg
    14461 ccatcttcgc cggctttctc ggaccgcttg ccggcgcgcg cgcgtcagag gtcgtcacca
    14521 cgacgctcgt gctgatcacg gccctcctct cctggctcgc cttctacgag gtcgcccttc
    14581 agggccagga gcagcgcatc gagctcgcgc ggttcatcgt ctcgggcgac ctcaagtcca
    14641 tctggtcgct caggatcgat acgctgaccg cggtgatgct cgtcgtcgtg agcacggtct
    14701 ccggcctcgt gcatctctac tccatcggct acatggaaga ggatgacggc cggccgcgct
    14761 tcttcgccta tctgtcgctc ttcaccttcg ccatgctcgc gctcgtcacg gccgacgatt
    14821 ttgttcagct cttcttcggc tgggagggcg taggcctcgc ctcctatctc ctcatcggct
    14881 tctggtatca caagccgtcg gcgaatgcgg ccgccatcaa ggccttcatc gtcaaccgcg
    14941 tcggcgattt cggcttcgcg ctcggcattt tcgccgtgtt cgcgctgttc gactccgtcc
    15001 aatacgatgc ggtgttcgcc tcggctccta cccatgtcgg cgagaaggtg aatttccttt
    15061 ggatgcaggt cgatgcgctg acgctgatct gcctcctcct gttcatgggg gccatgggca
    15121 agtcggcgca attcctgctc cacacctggc tgcccgacgc catggagggc ccgacgcccg
    15181 tctcggccct gattcatgcc gcgaccatgg tgacggccgg cgtgttcatg gtggcgcggc
    15241 tttcgcccct gttcgagctc gcgccgatcg cgctcgccgt cgtgacgatc gtcggcgcca
    15301 ccaccgcctt cttcgcggcg accgtcggat tggtgcagaa cgacatcaag cgggtggtgg
    15361 cctattcgac ctgctcgcag ctcggctaca tgttcgccgc cgaaggcgtc ggcgcttatg
    15421 gcgtcggcat gttccacctc tttacccacg cctccttcaa ggcgcttctg tttctgggcg
    15481 caggcgccgt aatccatgcg ctgcatcacg agcaggatct ccgccgcatg ggcgcgctgc
    15541 ggcgcgtttt gcccttcacc tgggcgatga tgctgatcgg cacgctgtcg ctcaccggct
    15601 tcccgttcac cgccggcttc gtctccaagg acgccatcat cgagtcgacc tatgcggcgc
    15661 attcctgggt tggcatgtac gccttcgtgc tcaccgtcgt cgccgccgtg ctgacctcgt
    15721 tctattcctg gcggctgatg tttctcgctt tcgagggcaa gccgcgagag ccgaaggagc
    15781 tgctggagca cgcgcacgag ccgaacttga ccatgaccgt cccgctcgcc gtgctcgctc
    15841 tcggtgcgct cctcacaggc ggcctgttcg cgcatctctt catcgggacg gagcaggccg
    15901 agttctggcg cggttctctc gtcgtgcatg agcatcatca cgtcccgctt tgggtcgtgc
    15961 tcgcgccgtc cgtcgccatg gcgcttggct tcgccgtcgc ctggtacttc tacatcacca
    16021 acccgctcgt gcccttcggc ctcgccaagc gattccgcgg cgcctaccag ttcctgctca
    16081 acgcctggta tttcgatgcg ctgtacgacg cgctgttcgt taggcccgcg cttcggctcg
    16141 gccgcttcct gtggaagacc ggcgacggca ccatcatcga cggcatcggt cccgacggcg
    16201 tcgccgcgcg cgtcatcgac atcgccaacc gcgccatcag gcttcagacc ggttacatct
    16261 atcactacgc cttcgtcatg ctgatcggcg tggcgttgct catcacctat tttctgttcg
    16321 ccggcggagg gctcaacccg tgagctatag ctggccgatc ctgtccacca tcacctttct
    16381 gccgctgctt ggcgcgctgc tgatctgtct cttgcggggc ggagatgaga gcgccttccg
    16441 c
//
LOCUS       DASUQP010000242         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1346068, whole genome shotgun sequence.
ACCESSION   DASUQP010000242 DASUQP010000000
VERSION     DASUQP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346068"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..665)
                     /locus_tag="VFY74_11250"
     CDS             complement(156..665)
                     /locus_tag="VFY74_11250"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX5932305.1"
                     /translation="MYYHAISQCTQALRNLEAWLDKAEQHAATKKFDVGVLITGRLAP
                     DMKPFVYQVQSACDYTKAAAAWLSGQTPPRHEDNEQMIDELRARIRKTVAFAESVQEA
                     QYAGAADRTVSLSFAPTGKVLGGEDYLLQMTIPNVYFHIAMAYAILRHNGVEIGKMDF
                     LGPINWIDA"
     gene            complement(767..1912)
                     /locus_tag="VFY74_11255"
     CDS             complement(767..1912)
                     /locus_tag="VFY74_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003588285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX5932306.1"
                     /translation="MVTLARNKLFTPVHIGAITLKHRVVMPPLSRLRAEWPSGVPSNL
                     MLEYYSQRASDGGLIFTEAIAVSPSARGYRGAPGIYSDEQVTGWQRVTGAVHAKGGYM
                     FAQLWHAGRTTHVAVTGEEPVTASVDPTYWADPSILVVTPDGFSQPSPHRALETTEIA
                     GIIEQYRAAALNAKKAGFDGVELMAANGHLIDQFLQDNSNKRTDRYGGSIENRARLLF
                     EVAEALVSVWGPNRVGVRVAPSGTFNGMADSDPRALFRYVAERLNGLNLAYLHVIEPR
                     IKGGELIAEGMGPVAAQELGQIFNGPVIAAGGFEPDAAEAAVASGVASLIAFGRHFIA
                     NPDLPKRIELGLPLNHYDRSTFYGFDARGYTDYPTYEASELAQVAVA"
     gene            complement(2008..2781)
                     /locus_tag="VFY74_11260"
     CDS             complement(2008..2781)
                     /locus_tag="VFY74_11260"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008531210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5932307.1"
                     /translation="MRFQDKIAIVTGGGSGMGKGVATRFVAEGGSVVVNGRDAAKTEA
                     AARQIDPSGKRAVAYVGDVALPATGEALVKTAIDRFGRLDVLFNNAGIFAPKPFLEVT
                     EAEYDRFLDNILKGTFFTAQAAAKAMKAAGRGGAIVQTGSMWALQAIGATPSSAYSAA
                     KAGVHALVKNLAIELAPDKIRVNAIAPAVIETPVYDTFLTPEQVKAVLPTFNAFHPLG
                     RNGQVADAAEALLFLASDQASFITGVVLPVDGGVMAGRQ"
     gene            2890..3405
                     /locus_tag="VFY74_11265"
     CDS             2890..3405
                     /locus_tag="VFY74_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008531211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5932308.1"
                     /translation="MKETAERLMDLAEAHIRNAGYAGFSFRALAAEIGIKSASVHHHF
                     PTKATMAAAVARRYADRFFAAVARRPNETADDAIAAYRSTFKAALDRDGLMCLFGVLG
                     AEAGGLSPEIADEILSFFRRCVEDLSQRIGGPDAETRAFQVIATLEGGMMLARAYQDT
                     EVFDQAVAALV"
     gene            3412..4103
                     /locus_tag="VFY74_11270"
                     /pseudo
     CDS             3412..4103
                     /locus_tag="VFY74_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          786 a         1416 c         1391 g          800 t
ORIGIN      
        1 attccaagcg cagtgcaatg cggcaacaaa aggggctggc gaccatgtcg gcgggccgcc
       61 agcttagctt gctctcgtaa tgaggacttc gccccggcgc ggcggctctg ctccgcagat
      121 cccggagatc agtgcagcaa aagatctcag acgagtcacg cgtcgatcca attgatcgga
      181 ccaaggaagt ccatcttgcc gatttcgacg ccgttgtgac ggaggatcgc gtacgccatc
      241 gcgatatgga aatagacgtt tgggatcgtc atctgcagca ggtagtcctc gccgccaaga
      301 accttgccgg tcggcgcaaa ggacagactg accgtccggt cggcggcacc ggcatactgc
      361 gcctcctgca cgctctccgc gaaagcgaca gtcttccgga tacgggcgcg caactcgtcg
      421 atcatctgct cattgtcctc gtgcctaggc ggcgtctgcc cagacagcca agcggcggcc
      481 gccttggtat agtcgcacgc gctttgaacc tggtagacga agggcttcat gtcgggcgcg
      541 aggcggccgg ttatcagcac cccgacgtcg aacttcttgg tggctgcatg ctgctccgcc
      601 ttgtcgagcc aggcctcgag gttcctcagc gcctgcgtgc attgcgagat cgcgtggtag
      661 tacatggatc cttccttccc gttcggtgcg ctcgtggaat tctaaagact gtagatcaag
      721 gtcctagccg gagacgacgt gcgggccagg accttctcgc cgtggctcag gcaacagcca
      781 cttgagccaa ttcactcgct tcataggtgg ggtagtcggt gtacccgcgc gcatcgaatc
      841 catagaaggt gctccggtcg tagtgattga gcggcaggcc aagctcgatg cgcttcggca
      901 ggtccggatt ggcaatgaag tggcggccga acgcgatcag gctggcgacg ccactggcga
      961 cggcggcctc agccgcatcg ggctcgaagc cgccggctgc gatgaccgga ccgttgaaga
     1021 tctgccccag ctcctgcgcg gcgaccggcc ccatgccttc cgcgattagc tcgccgccct
     1081 tgatccgggg ctcgatgacg tggagatagg cgagattaag gccgttgagg cgctccgcga
     1141 cgtaacggaa cagggcccgc ggatcgctgt ccgccatgcc attgaaggtg ccgctcggcg
     1201 cgacgcgcac gcccacccga ttcgggcccc agaccgagac cagcgcctcg gcgacctcga
     1261 agagcagccg ggcccggttc tcgatggagc cgccatagcg gtcggtccgc ttgttgctgt
     1321 tgtcctgaag gaactggtcg atcagatggc cattggcggc catgagttcg acgccgtcga
     1381 agccggcctt cttcgcgttg agagcagcgg cgcggtattg ctcgataatc ccggcaatct
     1441 cggtcgtttc cagcgcccgg tgcggcgatg gctgcgaaaa gccgtccgga gtgacaacga
     1501 ggatgctcgg atctgcccaa taggtcggat cgactgacgc agtgacaggc tcctccccgg
     1561 tcacggcgac atgggtcgtg cggccagcgt gccagagctg ggcgaacatg tagccgccct
     1621 tcgcatgcac cgcacctgtt acgcgctgcc agcctgtgac ctgctcgtcg ctataaatcc
     1681 ccggagcgcc ccggtatccc cgcgccgacg gcgagacggc gatcgcctcc gtgaagatga
     1741 gcccaccatc cgaggcgcgc tggctatagt actccagcat caggttgctc ggcacgccac
     1801 tcggccactc ggcacggaga cggctcaagg gcggcatcac aacgcgatgc ttgagtgtga
     1861 tcgccccgat atgcacgggg gtgaagagct tgttgcgagc aagcgtaacc atttcgattc
     1921 tccttggaga gcgggcggcg ctggcgcact cggtgtcgcc agacgcggcc cgctatgtcc
     1981 tgggtgagat gatcggtgtt ccggtgatca ctgacggccg gccatgacgc cgccgtcgac
     2041 gggcagcacg acgccggtga tgaagctcgc ctggtccgac gcgaggaaca gcagcgcttc
     2101 cgccgcatcc gccacttggc cgttgcggcc gagcggatgg aacgcattga aggtcggcag
     2161 cacggccttg acctgctcgg gcgtcaggaa cgtgtcgtac acgggcgtct cgatcactgc
     2221 cggcgcaatc gcattcacgc ggatcttgtc cggcgccagc tcgatggcga gattcttgac
     2281 cagggcgtgt acgccggcct tcgccgctga ataggctgag gatggcgtgg cgccgatcgc
     2341 ctgcagcgcc cacatcgaac cggtctgcac aatggcaccg ccccggccgg ctgccttcat
     2401 ggccttcgcg gcggcctgtg cggtgaagaa cgtgcccttg aggatgttgt cgaggaaccg
     2461 atcgtactcg gcctcagtca cctcgaggaa cggcttcggc gcgaagatac ctgcgttgtt
     2521 gaatagcacg tcgagacggc cgaatcgatc gattgcggtc ttcacgaggg cctcaccggt
     2581 ggccggaagg gctacatcgc cgacgtacgc gacagcacgc ttgcctgagg gatcgatctg
     2641 acgggcggcc gcttcggtct tggccgcatc gcgaccgttg acaacgaccg agccgccctc
     2701 ggcgacgaac cgggtcgcga cccccttgcc catccccgaa ccaccgccgg tgacgatggc
     2761 aatcttgtcc tggaaacgca tggcttttct ccgcagttta tctacctatc gataggttga
     2821 tacggcacaa atagctctgg acatctcggc cgtcaatatc tatctatcga tagacagtgg
     2881 agcgcggccg tgaaagaaac cgccgaacga ctgatggacc tggcagaggc ccatatccgc
     2941 aatgccggat atgccggctt cagctttcgc gctcttgccg ccgagatcgg catcaagagc
     3001 gcgagcgttc atcaccattt tccgaccaag gccacgatgg ctgctgctgt cgcgcgacgt
     3061 tatgcggacc gctttttcgc cgcggttgcg cggcggccga atgagactgc cgacgacgct
     3121 atcgccgctt accggtcaac cttcaaagcg gccctcgatc gagacggcct aatgtgcctc
     3181 ttcggtgtgc tgggggctga agcgggtggt ttatcacccg aaatcgccga tgaaatactg
     3241 tccttcttcc gccgatgcgt tgaggaccta tcccagcgga tcggcgggcc cgatgcggaa
     3301 acgcgggcct ttcaggtcat tgcaacgctc gaaggcggca tgatgctcgc gcgtgcctat
     3361 caagacaccg aagtgttcga ccaggcggtt gccgccctcg tatagcccct cctgaccatc
     3421 ggcgaggcga agctattccg gtccccgaag accatcatac ggcacgcgat ctggctcttt
     3481 ttgcgttcac cctcagcctt cgcgacgtcg aggaatgact tgccgagcga gggatcatcg
     3541 tcacctacga gactgtccgg gtctggatcg ggcgcctcgg accgctgttc gcaaggcacc
     3601 tgcggcgccg tcgcggtcgg ccgagcaaca tctggacgag atattcgtga agatcgccgg
     3661 tcggaccatg cacctgtggc gggccgtcga cggagggggc gaggtcctcg acatgttggt
     3721 gtagagccga cgcgacaagc gtgctgctct acgactgatg cgtatgctgc tgaagaagca
     3781 gagcatagtg ccggaggagt tggtgaccga ccggctgcgg gcctacggtg ccgcgatgcg
     3841 tgagctgggc ctcagcgcgg agcacatcca gggcaagcgc aagaacagcc gggcggagag
     3901 ctcgcacgtc ccgatccgac gacgagagcg cgagatgcag gggtttcgat cgcccggatc
     3961 gacccaacgt ttcctcgctg cgcacgccgc cgtcgccaac accttcacca cctgccgtca
     4021 cctgatctcc gcccccctcc catcgccaga ttcggccgaa ggccctcgtc gcatggcgcg
     4081 agaccgcaaa actttgggtg tgatcattgc tggcgcaccg atcgctgcgc gcttaccccg
     4141 tcaacccgac aaaccccgcc gcgcagctgt aggccgagag gcgagatcgg cgagacgggc
     4201 tgtccgccag gcggatgagc gctcgtcatc gctgtgcgcg cggggtcgcc tcgccccaag
     4261 ctctccgctg ctccccccat tcgagcgtcc cattgcccag catctcagcc agcctgaggc
     4321 ctctcggctg ccgcccgtca agcatcgctt ccacaatatc tggcgccaag caggtgagtg
     4381 ggaggagccg gca
//
LOCUS       DASUQP010000243         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_256912,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000243 DASUQP010000000
VERSION     DASUQP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256912"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /gene="metG"
                     /locus_tag="VFY74_11275"
     CDS             complement(<1..1526)
                     /gene="metG"
                     /locus_tag="VFY74_11275"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5932309.1"
                     /translation="MADKRPYYITTAISYPNDAPHIGHAYEAVATDALARFRRLQGHD
                     VFFLTGTDEHGIKMLQTAKGQGITPAELADRNTPKFRDMVRVLNCSNDDFIRTREERH
                     KLACQELWRRMQAAGDIYRDKYAGWYSVRDEAYYDESELKEGTGGSKLSPQGTPVEWV
                     EEESYFFRLSAYQDKLLAYYEAHPEFIGPETRGNEVKNFVRGGLQDLSISRTTFDWGI
                     PVPGDAKHIMYVWVDALTNYLTGVGFPEEGSETFRRYWPADVHIIGKDIVRFHAVYWP
                     AFLMSAGVALPKQVFGHGFLYNRGEKMSKSVGNVVDPFTLAKDYGTDQLRYFLLREVP
                     FGQDGNYSREGIVQRINADLANDLGNLAQRSLSMIAKNCSGKMPKPGGFTAADETLLA
                     SADALLPRVEAAIDEFAIHRALEMIWAVIADANRYFASEEPWAHKKTDPERMATILYV
                     TAEVTRQIAILVQLSMPESAGRLLDQLGVPQPARDFAALGPKGRLKAGTKLPPPMPVF
                     P"
     gene            complement(1582..2700)
                     /locus_tag="VFY74_11280"
     CDS             complement(1582..2700)
                     /locus_tag="VFY74_11280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX5932310.1"
                     /translation="MSVVAERARAALTEPDRLEGFSLPREIDRLFGHEAALHEFHDAL
                     ESGRMHHAWLIVGPEGVGKATLAYRFARTVLAEGERGDVRTPIDRDHPVFRKVAALSH
                     PNLLLIRRSWVEKSKRYSQWIGVEEVRRLRAFLGHSAGGGGWRVVIVDRADELNQNAA
                     NALLKALEEPPPKTLFLLISSAEGRLSVTIRSRTRTLRVTALGKEELESAVRAALVRD
                     GHETDDETLAIALALSEGSVRRALELVSNGGIELYREILVALGGLPEIEGPELHRLVD
                     RLSGAAESERFDLYLALLLGLIERLIRFAATGTRATAEEQRLAARLLSPANLAHWAEA
                     WEAISEAKAEAQALNLDRGILLLESWFRLQQLAREHPV"
     gene            complement(2697..3347)
                     /gene="tmk"
                     /locus_tag="VFY74_11285"
     CDS             complement(2697..3347)
                     /gene="tmk"
                     /locus_tag="VFY74_11285"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5932311.1"
                     /translation="MQRGTGEGGRFITFEGGEGSGKSTQAGILANRLTRAGRKVFATR
                     EPGGSPAAEEIREALLSGRVSQFGPFAEAVMFAVARADHIEKAIGQALHAGKWVVCDR
                     FYDSTRAYQGATAGVPRGLINALERLTVGTLSPDITFILDIRPEQGLARAAERRNGTA
                     PDRFESQQLMQHERIRRAFLDIAEEEPDRCVVIDASQPEAMVAEDVWEVVLQRLHP"
     gene            complement(3350..>4613)
                     /locus_tag="VFY74_11290"
     CDS             complement(3350..>4613)
                     /locus_tag="VFY74_11290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5932312.1"
                     /translation="LDICCVWPRVVAAKTPSKPGQPVHAPRLDKRIKIAAIGAVLFFG
                     AALVAATLQAAPPPVFETKAQHAILMDAGADLVLFEKDADTLAPPASMSKLMTLAIVF
                     RELKAGRLKLEDQFKVSEHAWRTGGAPSGGSAMFAPLNTMVTVSDLIQGVTVQSANDA
                     AIILAEGIGGTEDAFAQQMTDYSRKIGLEKSTFANATGLSAEGHLMTARDLATLAHHL
                     IYTYPEYYHFFGQREFKYREKFTFLNRNPLIFADIGVDGLKTGFIKESGYGMVASGKR
                     GDQRLVVVVGGLESAKERETEARRLLDWGYKSFRPFRLFDAGQKVSDALVWGGTQHYV
                     PLVGDGDINIILPANATGKVSAEIVYQGPIKAPIRKGDEVAVLKITSAEAQATNEIPL
                     YAGEDIGQSNFAMRGIDSLLVLAFGWLL"
BASE COUNT          788 a         1515 c         1489 g          821 t
ORIGIN      
        1 ggaaacacag gcatgggcgg cggcagcttc gttccggcct tgagcctccc tttaggtcct
       61 agcgcggcga agtcgcgcgc cggttgaggc acgccgagtt ggtcgagcag cctgcccgcg
      121 ctctccggca tcgacagctg gaccaggatc gcgatctgcc gcgttacctc cgccgtgacg
      181 tagaggatcg tcgccatccg ctccggatcg gtcttcttgt gcgcccaagg ctcctcgctc
      241 gcgaaatagc ggttggcgtc ggcgattacc gcccagatca tctcgagcgc ccgatggatg
      301 gcgaactcgt cgatcgcggc ctcgacacgc ggtaggagcg cgtcggccga ggccaacagg
      361 gtctcgtcgg ccgccgtgaa cccgccgggc ttcggcatct ttccactgca gttcttggcg
      421 atcatcgaca gcgagcgctg ggcgagattg ccgagatcgt tggcgaggtc ggcgttgatg
      481 cgctgcacga tcccctcgcg gctataattt ccgtcctggc cgaacggcac ctcgcgcagg
      541 aggaaatagc ggagctggtc ggtgccgtaa tccttcgcga gcgtgaacgg atcgacgaca
      601 ttgccgaccg atttcgacat cttctccccg cggttataga gaaagccgtg gccgaacacc
      661 tgcttcggca gcgccacgcc cgccgacatg agaaacgcgg gccagtagac ggcgtgaaag
      721 cggacaatgt ccttgccgat gatatgcaca tcggccggcc agtagcgccg gaacgtctcc
      781 gacccctcct ccggaaagcc gacgccggtc aaatagttgg tcagcgcgtc gacccacaca
      841 tacatgatgt gcttcgcatc gccgggcacg ggaatgcccc aatcgaaggt cgtgcgcgag
      901 atcgagagat cctggagtcc gccgcgcacg aagttcttca cctcgttccc gcgcgtctcc
      961 ggaccgatga actcgggatg cgcctcgtaa taggcgagca gtttgtcctg gtaggccgag
     1021 agccggaaga aatagctctc ctcctcgacc cactcgaccg gcgtgccctg cggcgagagc
     1081 ttgcttccgc cggttccctc tttcagttcg ctctcgtcgt aataggcctc gtcgcgcacc
     1141 gagtaccacc ccgcatattt gtcgcgatag atgtcgccag ccgcctgcat gcgccgccac
     1201 agctcctggc aggccagttt gtgccgctcc tcgcgggtgc ggatgaaatc gtcgttcgag
     1261 caattgagca cgcggaccat gtcgcggaac ttgggcgtgt tccgatcggc gagctcggcc
     1321 ggcgtgatgc cctggccctt cgccgtttgc agcatcttga tgccgtgctc gtcggtcccg
     1381 gtcaggaaga acacgtcgtg gccctgcaag cggcggaagc gcgcgagcgc atcggtggcc
     1441 acggcctcat aggcgtggcc gatatggggc gcgtcgttcg gataggagat ggcagtggtg
     1501 atgtaatagg gcctcttgtc tgccatgacg gcaggaatag ccctaaggcc cggccgcggg
     1561 caagcgcgct cgcttctctc gctatacagg gtgttcccgg gccaattgct gcagccggaa
     1621 ccagctctcg agcaaaagga tgccgcggtc gagattgagg gcctgcgcct cggccttggc
     1681 ttccgagatt gcctcccacg cttcggccca atgagcgaga ttggccggcg aaaggagccg
     1741 cgcggcaagc ctctgctctt ctgccgtcgc ccgcgtcccc gtcgccgcga aacggatcag
     1801 ccgctcgatc agaccgagga gaagcgcaag atagagatcg aaacgttcgc tttcggcggc
     1861 gccggagagc cggtcgacga ggcggtgaag ctccgggcct tcgatctccg gcagtccgcc
     1921 aagggcgacg aggatctcgc gatagagttc gatgccgcca ttggaaacga gttcgagcgc
     1981 gcgccgcacg ctcccctcgc tcagcgcgag cgcgatggcg agcgtctcat cgtcggtttc
     2041 atggccgtcg cggacgagcg cggcccgcac cgcgctctcc agttcctcct tgcccaatgc
     2101 cgtcacgcga agcgtgcggg tgcgtgagcg gatggtgacg gaaagccgcc cctcggcgct
     2161 tgaaatgagg agaaagaggg tcttgggcgg cggctcctcg agcgccttca ggagcgcgtt
     2221 cgccgcgttc tggttcagct cgtcggcgcg atcgacgatg acgacccgcc agcccccccc
     2281 tcccgccgaa tgtccaagga aggcgcgcaa gcgccgaacc tcctcgacgc cgatccattg
     2341 cgaatagcgt ttcgacttct cgacccagga gcgccgaatg agaaggaggt tgggatgcga
     2401 cagggccgcg accttgcgga acacgggatg gtcgcgatca attggggttc gcacgtcgcc
     2461 ccgctcgcct tcggcaagga ccgtgcgggc aaagcgatag gcaagcgtcg ccttgccgac
     2521 gccctccggc ccgacgatga gccaggcatg gtgcatgcgt ccgctttcca gcgcgtcgtg
     2581 gaactcgtga agcgccgcct catggccgaa caggcgatcg atctcgcggg gcaatgagaa
     2641 cccttcgagc cgatcgggct ccgtgagcgc cgcgcgcgcg cgctcggcca ccacgctcat
     2701 gggtgtaggc gctggaggac gacttcccaa acatcctctg cgaccatggc ctcgggctgg
     2761 ctcgcatcga tcaccacgca gcggtccggc tcttcctcgg cgatatcgag gaaggctctg
     2821 cggatgcgct catgctgcat cagctgctgg ctctcgaagc gatcgggtgc ggtgccgttg
     2881 cgacgttcgg ccgcacgcgc gaggccctgt tcggggcgga tgtcgagaat gaaggtgatg
     2941 tcgggtgaaa gagtcccgac ggtgagacgt tccaacgcgt tgatcagccc gcgcggcacg
     3001 ccggcggtcg ccccctgata ggcgcgcgtg gaatcgtaga agcgatcgca cacgacccat
     3061 ttacccgcgt gcagcgcctg cccgatcgcc ttctcgatgt gatcggcccg cgcaaccgcg
     3121 aacatcaccg cctcggcaaa gggaccgaat tgcgagacgc ggcccgagag cagcgcttcg
     3181 cggatctcct ccgcggccgg cgagccgccc ggctcgcgcg tggcgaagac ctttcgtccg
     3241 gcgcgggtca ggcgattggc aagaattccg gcctgcgtgg acttgcccga gccctcgccg
     3301 ccttcgaacg ttatgaatct cccgccttcg ccggtcccgc gctgcatgac tacaacagcc
     3361 agccgaaggc aagcacgagc aacgagtcga tccctcgcat ggcaaagttg ctttgcccga
     3421 tatcttcgcc ggcatagagc gggatctcgt tggtcgcctg cgcctcggct gacgtgatct
     3481 tcagcaccgc cacctcgtcg cccttgcgga tgggggcctt gatcggccct tgatagacga
     3541 tctccgccga aaccttgccg gtcgcgttcg cgggcaagat gatgttgatg tcgccatcgc
     3601 cgaccagcgg cacgtagtgc tgcgtgccgc cccacaccag cgcatcgctc accttctggc
     3661 ccgcgtcgaa caaacggaac ggcctgaagc tcttgtaacc ccaatcgaga agccggcgcg
     3721 cctcggtttc gcgctccttc gccgattcga gcccgccgac gacgacgaca agccgctgat
     3781 cgccgcgctt gccgctcgcc accattccgt atcccgactc cttgatgaac ccggtcttca
     3841 agccgtccac gccgatatcg gcgaagatca gcggattgcg gttcaagaac gtgaacttct
     3901 cgcgatactt gaactccctc tgtccgaaga aatgatagta ctcggggtag gtgtagatca
     3961 gatggtgggc gagcgtcgcg aggtcgcgcg cggtcatcag atgaccttcg gccgacagcc
     4021 cggtggcgtt ggcgaaggtc gatttctcga gcccgatctt gcgggaatag tcggtcatct
     4081 gctgggcgaa ggcgtcttcc gtgccgccga tcccttcggc gaggatgatg gcggcgtcgt
     4141 tcgccgactg gacggtgacg ccctgaatga gatcgctcac cgtcaccatc gtgttcagcg
     4201 gcgcgaacat ggcggacccg cccgacggcg cgccgccggt ccgccacgca tgctcgctga
     4261 ccttgaattg gtcctcaagc ttgagccgcc cggccttcag ctcgcggaag acgatggcga
     4321 gtgtcatcag cttgctcatg ctcgccggcg gagcgagcgt atcggcgtcc ttttcgaaca
     4381 gcacaagatc ggcgccggca tccatgagga tggcgtgctg cgccttggtc tcgaacaccg
     4441 gcggaggcgc cgcctgcagc gtcgccgcga cgagggcagc gccgaagaag aggacggcgc
     4501 cgatcgctgc gatcttgatc cgcttgtcaa ggcgcggggc gtgcacgggc tgaccgggtt
     4561 tggagggagt tttggccgca acgacgcgcg gccacacgca gcaaatatcg agc
//
LOCUS       DASUQP010000244         7802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_667310,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000244 DASUQP010000000
VERSION     DASUQP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667310"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..378)
                     /locus_tag="VFY74_11295"
     CDS             complement(55..378)
                     /locus_tag="VFY74_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX5932313.1"
                     /translation="MGTRVSAVSRLNLIAVAAVLCCLGTSPAAAGCSKSVVMYNASWC
                     PYCRQVRAILARNHIKYSMLDATTAPVQADMIRRFGDTAVPRTIIGGALVEGVDEARI
                     KQLCR"
     gene            489..1295
                     /locus_tag="VFY74_11300"
     CDS             489..1295
                     /locus_tag="VFY74_11300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5932314.1"
                     /translation="MLIDSHCHLDFPDLVRDLDSVLARARQAGIGLIVTISTRVRRFD
                     QLKAIVEAHPQVFCSIGTHPHNAAEEPDVTAEELIAIARHPKVVAIGEAGLDYHYDHS
                     PRDVQAKSFRTHIEAARDTGLPLIIHAREADGDIARILEEESAKGAFPFVLHCFTSGL
                     DLARRGLALGGYVSFSGVVTFKNAGALRDIALAVPEDRLLVETDAPYLAPEPMRGKTN
                     EPAFVTYVAARLAPLRGLGESELARLTTENFFRLFRKVPRSLQKKPQSAA"
     gene            1292..2092
                     /locus_tag="VFY74_11305"
     CDS             1292..2092
                     /locus_tag="VFY74_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5932315.1"
                     /translation="MTLKVTILGCGTSGGVPRVGNHWGACDPADPKNRRKRCALLVER
                     NGAEGKTTVLVDTPPDLRQQLLDAGAGWLDGVLYTHDHADHTHGIDDLRQVAYNGNRR
                     VDVYHDEATGKQLRARFTYCFETPPGSEYPAILKAHDIRASEAVRIAGAGGIIETLPF
                     RQIHGSGETLGFRFGGIAYSPDVSDFPDASLDALQGLDVWILDTLRYKPHPSHLSVEQ
                     ALAWIARMKPKRAVLTHMHVDLDYARLRAELPEGVEPAYDGMVLQAEG"
     gene            complement(2098..2805)
                     /locus_tag="VFY74_11310"
     CDS             complement(2098..2805)
                     /locus_tag="VFY74_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5932316.1"
                     /translation="MSSERLKDRIALITGASRGIGRAVALAFAREGAHILLLARARKA
                     LEEVDDRIRAGGGKTTLVPLDLADGKAIDALGPSLYERFGRLDVLVGNAAILGRLSPL
                     THVPSELWDKSFAVNVTANWRLIRTLDPLLRRSDAGRVIFVTSGVARSARAYWAPYAV
                     TKAALEALARIYASEASDSAIKVNVIDPGATATRMRAEAYPGEDQSKLRTPEQVAEAF
                     VPLAMPDLADTGQIVEL"
     gene            complement(2802..4274)
                     /gene="purF"
                     /locus_tag="VFY74_11315"
     CDS             complement(2802..4274)
                     /gene="purF"
                     /locus_tag="VFY74_11315"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5932317.1"
                     /translation="MTDHVETKEPSWLEEDRLHEACGVFGILNHPDAAALTALGLHAL
                     QHRGQEAAGIVTFDGHHFHTERRLGLVGDHFTSRPVIDRLKGDMAIGHVRYSTCGDPI
                     LRNVQPLFVDLDSGGFALAHNGNLTNALILREALIKRGAICQSTTDTEVILHLVARSE
                     KQRFIEKFIEALLQVEGAYALVGLTHKKLIGARDPFGIRPLVIGKLGEAWVLASETCA
                     LDIIGAEFVREVENGEVVVMTHEGVESHRFVPKYPARLCIFEYIYFARPDSIVGGRSV
                     YEVRKEMGRQLAREAPAHADVVIPIPDSGVPAAIGYSQVSGLPFEFGIIRNHYVGRTF
                     IEPEQRIRQLGVKLKHSANEGQVQGKRIVLIDDSVVRGTTSVKIVQMMREAGASEVHM
                     RVSSPPITHPDFYGIDTPDQDKLLAAHRTLEEMRTFIGADSLQFLSVEGIYKSMGYDG
                     RDNAHPQFTDHCFTGEYPTRLRDREGPPQQKQLSFLAEVG"
     gene            complement(4362..4970)
                     /locus_tag="VFY74_11320"
     CDS             complement(4362..4970)
                     /locus_tag="VFY74_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX5932318.1"
                     /translation="MPISWLDVILIAIMLISGFLAMVRGFTREVLSIFSWAVAAVAAL
                     YLTPKYANLLEPYVGNPSIAQIAFAAGVFIITLIVVSLITFRISDSVLDSRVGALDRT
                     FGFIFGLARGFLLVAIVFILFTALARDQPDWVRNARSYSILQQTQVAIESLLPMNPEQ
                     YLPGTQDEQQEGQPGGQPEGAPGTSPEGRPEAPAEVPPDQPS"
     gene            complement(5094..6473)
                     /gene="radA"
                     /locus_tag="VFY74_11325"
     CDS             complement(5094..6473)
                     /gene="radA"
                     /locus_tag="VFY74_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5932319.1"
                     /translation="MAKASRAFVCQACGAVSNRWSGKCASCGEWNSIIEERAPTGSPG
                     LIAIKGGKGRLAALETLSAATSEAPRLPTGIAEFDRVLGGGLVPGSAVLVAGDPGIGK
                     STLLLQAAAALAASGAAVVYLSGEEAPAQIRMRAARLGLSAAPVALGAETNLANIVAT
                     LARGTPPALIVIDSVQTLWSEGVEAAPGTISQLRGCASALIGHAKASGSALILIGHVT
                     KDGQIAGPKVIEHMVDSVLSFEGLSGHQFRILRAVKNRFGPTDEIGVFEMREAGLKEV
                     VNPSALFLSDREQGAPGTAVFAGMEGTRPLLVEIQALVAPSSLGTPRRAVVGWDPNRL
                     AMLLAVLEARCGVRLGAHDVYLNVAGGLRLNEPAADLAAAAALMSSTSANPPPEDTVY
                     FGEVSLTGAVRAVSHMDQRLKEAAKLGFTSAVVPVAAGEGERETRDMRLLPLSQLDDL
                     VALFCTSRV"
     gene            complement(6482..7627)
                     /gene="alr"
                     /locus_tag="VFY74_11330"
     CDS             complement(6482..7627)
                     /gene="alr"
                     /locus_tag="VFY74_11330"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5932320.1"
                     /translation="MRAGEVPSGARDEGPGETAILTIDLDAVAANYRTLRDLASPAEC
                     AAVVKADAYGLGMVPISSTLSRIGCKTFFVATLDEGERLRALLPDAIIYVLAGLMRGT
                     AELYRAHHLRPVLNSADEVREWAAFCETQGERLDCAVHIDTGMNRLGLSAEDVDQVAR
                     AAELWSALTLSLVMSHLACADEPEHPKNGAQRSMFDRLRAQLPKAPSSLANSPGILLG
                     RAFFYDVVRPGIAIYGGHPRGHGHNPFRPVVQLKGRIMQVRDVPRGETVGYGATRTIR
                     EAARLAVVSVGYADGFARALSIADGEQGFSGYLGPHAAPIVGRVSMDLVALDVSHVPE
                     ELARRGAWVELIGPNVPAHVLAAHAGTIDYEVLTRLGQRAFRRYVGG"
     gene            complement(7633..>7802)
                     /locus_tag="VFY74_11335"
     CDS             complement(7633..>7802)
                     /locus_tag="VFY74_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5932321.1"
                     /translation="HKQWQTEMETVSGRAEIIIGKQRHGPTGTVHLQFTPEFTRFGNL
                     AAEERLPERRA"
BASE COUNT         1395 a         2513 c         2582 g         1312 t
ORIGIN      
        1 ttccacgcca tgtagaagaa ggcagcgaag ccgcggcgtc gtaaaattcg gcgtctagcg
       61 gcacaattgc ttgatgcgcg cctcgtccac gccttcgacg agcgcgccgc caatgatggt
      121 tcggggaacg gccgtgtcgc cgaagcgccg gatcatgtcg gcttggaccg gcgcggttgt
      181 cgcgtccaac atgctatact tgatgtggtt gcgcgccagg atggccctga cttgccggca
      241 ataagggcac caacttgcgt tgtacatgac gacgctcttg ctgcacccgg ccgcggcagg
      301 gctcgtaccc aggcagcaca gcactgcggc cacggcgatg aggttgaggc ggctaaccgc
      361 ggaaacccgt gttcccattc tttctccccg tgcgagggcg ggcgaggccc ttattgtcac
      421 tccaggctag atgcggcaca tagcaaactt agcagcttga aggctctggg tcttttctcg
      481 tttccgtgat gctgatcgac agccactgtc atctcgactt tcccgacctt gtgcgcgacc
      541 tcgactcggt cctcgcccgc gcgcgccaag ccggtatcgg cctgatcgtg accatctcga
      601 cgcgcgttcg ccgcttcgat caactcaagg ccatcgtcga ggcgcatcca caggtgttct
      661 gctcgatcgg gacacacccg cataacgccg cggaggaacc cgacgtgacg gcggaggagc
      721 tgatcgcgat tgcgcgccat cccaaggtgg ttgcgatcgg cgaggcgggg ctcgattatc
      781 actacgatca cagcccgcgc gacgtgcagg cgaagagctt ccgcacccac atcgaagccg
      841 cgcgcgacac ggggctgccg ctcatcatcc acgcgcggga ggccgacgga gacatcgccc
      901 gcattctgga agaggagagc gcgaagggcg cgtttccctt cgttctccat tgcttcacca
      961 gcggacttga tctcgcgcgg cgagggcttg cgctcggcgg ctacgtctct ttctccggcg
     1021 tcgttacctt caagaatgcg ggcgcgctcc gcgacatcgc gctcgccgtg cccgaagatc
     1081 gcctcctggt cgagacggat gcgccctatc tcgcgccgga gccgatgcgc ggcaagacca
     1141 acgagcctgc tttcgtgacc tatgtggcgg cgcggcttgc gcccttgcgc gggcttggcg
     1201 agagcgagtt ggcgcggctg acaaccgaaa acttcttcag acttttcagg aaggtcccgc
     1261 gcagcttgca aaagaagccg caaagcgccg catgaccctg aaggtgacca tcctcggctg
     1321 cggcacatcg ggcggcgtgc cccgtgtcgg caatcattgg ggcgcgtgcg atcccgccga
     1381 tcctaagaac cggcgcaagc gttgcgcgct gctcgtcgag cgcaacggcg ccgaggggaa
     1441 gaccacggtg ctggtcgaca ccccgcccga ccttcgccag cagcttctcg atgcaggcgc
     1501 cggctggctc gatggcgtgc tttacacgca cgaccacgcc gatcacaccc atggcatcga
     1561 tgatctgcgc caggtggcct ataacggcaa ccgccgcgtc gatgtctatc acgacgaggc
     1621 gaccggcaag cagctccgcg cgcgattcac ttattgcttc gagacgccgc cgggaagtga
     1681 atatccggcg atcctcaaag ctcacgacat tcgggcaagc gaagcagtcc ggatcgccgg
     1741 tgctggaggc atcatcgaga cgctgccgtt ccggcagatc cacggctcgg gcgaaacgct
     1801 cggctttcgc ttcggcggga tagcctattc gccggatgtg agcgatttcc cggacgcgtc
     1861 gctcgacgcg ctgcagggtc ttgatgtgtg gatcctcgat acgcttcgct acaagccgca
     1921 tccgagccat ctctccgtcg agcaggcgct cgcctggatc gcacggatga aaccgaagcg
     1981 cgcggtgctc acgcacatgc atgtcgatct cgactatgcg cgattgcggg ccgagctccc
     2041 ggaaggcgtc gagccggcct atgacggcat ggtgttgcag gccgaaggat aggggtccta
     2101 cagttctacg atttgtcccg tgtccgctaa atccggcatc gcgagaggga cgaacgcctc
     2161 ggcgacctgt tccggcgttc gcagcttcga ctgatcctcg cccggatagg cttcggcgcg
     2221 catgcgcgtc gccgtggcgc cgggatcgat cacgttcacc ttgattgcac tgtcgctcgc
     2281 ctcgctggca tagatcctgg ccagggcttc aagcgcggcc ttggtcaccg cgtagggggc
     2341 ccaataggcg cgcgccgacc gcgccacgcc ggaggtgacg aagatcacgc gccccgcatc
     2401 cgaacggcga aggagcggat cgagcgtgcg gatcagccgc caattggcgg tgacattgac
     2461 ggcgaagctc ttgtcccaga gctcggaagg gacatgggtc agcggcgaga gccggccgag
     2521 aatggcggca ttccccacca gcacatcgag cctgccgaac cgctcataaa gagagggacc
     2581 gagcgcatcg atggccttgc cgtcggcgag gtcgagagga acgagcgtgg tcttgccgcc
     2641 gccggccctg atgcgatcgt cgacctcctc gagcgccttg cgcgcgcggg cgagaagcag
     2701 gatatgggcg ccctcgcggg cgaaggcgag cgcaacagcg cggccgatcc cgcgcgaagc
     2761 gccggtgatg agcgcgatgc gatccttgag ccgttccgaa ctcaaccgac ctcggcgagg
     2821 aaggagagct gcttctgctg tgggggacct tcgcggtctc tgagccgcgt cggatattcg
     2881 ccggtgaagc aatggtcggt gaactgggga tgggcgttgt cccggccgtc atagcccatc
     2941 gacttgtaaa tcccctcgac cgacaggaat tgcagcgagt cggcgccgat gaaggtccgc
     3001 atctcctcga gggtcctgtg ggcggcgagc agcttgtcct ggtcgggcgt gtcgatgccg
     3061 taaaaatcgg gatgggtgat gggcgggctc gaaacccgca tatgcacttc gcttgcgccg
     3121 gcttcacgca tcatttgcac gatcttcacc gaggtcgtgc cgcgcaccac gctgtcgtcg
     3181 atcagcacga tgcgcttgcc ttggacctga ccttcattgg cgctgtgctt gagcttgacg
     3241 ccgagctgcc tgatgcgctg ctcgggctcg atgaaggtgc ggccgacata gtgattgcgg
     3301 atgatgccga attcgaaggg cagccccgag acctgcgaat agccgatcgc ggcgggcacg
     3361 ccggagtccg ggatgggaat gaccacgtcg gcgtgggccg gggcctcgcg ggccaattgc
     3421 cgtcccatct ccttgcgcac ctcgtagacg gaacggccgc cgacgatgga gtccggcctg
     3481 gcgaaataga tatattcgaa gatgcagagc ctggccgggt atttcggcac gaagcgatga
     3541 ctctccaccc cctcgtgggt catcaccacg acctcgccgt tctccacctc gcgcacaaac
     3601 tcggcgccga tgatatcgag ggcgcaggtc tccgaggcga ggacccacgc ctcgccaagc
     3661 ttgccgatca cgagaggcct gatgccgaaa ggatcgcgcg caccgatcag cttcttatgc
     3721 gtgagcccga cgagcgcgta ggcgccttcg acctggagca gggcctcgat gaacttctct
     3781 atgaaacgct gcttctcgct ccgcgcgacg agatggagaa tgacctccgt gtccgtggtc
     3841 gactggcaga tggcgcctcg tttgatgagg gcttcgcgca agatcaacgc attggtcagg
     3901 ttgccgttgt gagcgagcgc aaagccgcct gaatcgagat cgacgaacag aggctgcacg
     3961 ttgcgcagga tcggatcgcc gcaggtcgag taacgcacat ggccgatggc catgtcgccc
     4021 ttcagccggt cgatgaccgg gcgggaggtg aagtggtcgc cgacaagtcc aaggcggcgt
     4081 tcggtatgga aatgatgccc gtcgaaagtg acgatgccgg ccgcctcctg gccccggtgc
     4141 tgaagcgcat gcaggccgag cgccgtgagc gccgcggcat ccggatgatt caggatgccg
     4201 aagacgccgc acgcctcgtg cagccggtcc tcttcgagcc agctcggctc cttggtctca
     4261 acgtggtctg tcacgggcct cgagcctcct gttgagtaaa taggcgcccc agagggcgct
     4321 tcaatggcac gcgacgcgac gcaatttcct gaacgttaaa ctcatgacgg ctggtccggc
     4381 ggaacctcgg cgggcgcctc gggccgcccc tccggcgatg taccgggcgc cccctcaggc
     4441 tggcctccag gctgcccctc ctgctgctcg tcctgagttc cgggcaaata ctgctcgggg
     4501 ttcatgggca gcagcgattc gatggcgacc tgcgtttgct ggagaatcga ataggagcgc
     4561 gcattgcgca cccagtccgg ctggtcgcgc gcgagcgccg tgaacagaat gaagacgatg
     4621 gcgaccagga gaaagccgcg cgccaacccg aagatgaaac cgaacgtgcg gtcgagagcg
     4681 ccgacccggc tgtcgagcac gctgtcggaa atgcggaatg tgatcaggct caccacgatc
     4741 agcgtgatga tgaaaacgcc ggcggcgaag gcgatctggg cgatgctcgg attgccgaca
     4801 tagggctcca gcaaattcgc gtatttcggc gtgagataga gcgctgcgac ggcggccacg
     4861 gcccaggaga agatcgacaa gacctcgcgg gtaaaccccc gcaccatggc gaggaagccc
     4921 gagatgagca tgatggcgat cagaatgaca tcgagccagg agataggcat tggcgcgagg
     4981 ctccttcggc gggcggcgca cactatcacg attcccggcg atgaagcaca aatggaccct
     5041 ctcggcaatc gttgaggctt tcgccggcgg gattgtcgca gggccgcccg aatctacacc
     5101 cgagacgtgc aaaaaagcgc cacaaggtcg tccaattgag acagcggcag gaggcgcatg
     5161 tcccgggtct cccgctcccc ttcgccggcc gcaacgggaa ctactgcgct ggtgaacccg
     5221 agcttggctg cctccttgag gcgctgatcc atgtggctta cggcgcggac ggcgccggtc
     5281 aggctgacct cgccgaaata gacggtgtcc tccggcgggg gattggcgct cgtcgacgac
     5341 atcagcgccg ccgcggcggc gaggtcggcg gccggctcat tgaggcgcaa gccgccggca
     5401 acgttgagat aaacatcgtg agcgccaagc ctcacgccgc aacgcgcttc aagcaccgcg
     5461 agcagcatgg cgaggcggtt gggatcccat cccaccacgg cgcggcgcgg cgttccgagg
     5521 gacgagggcg cgaccagcgc ctggatttcg acgaggagcg gccgcgtccc ttccatgccg
     5581 gcgaacacgg ccgtgccggg cgcgccctgc tcgcggtcgc tgaggaacag tgcggacgga
     5641 tttaccactt ctttcagccc ggcctcgcgc atctcgaaca cgccgatctc gtcggtcggc
     5701 ccgaagcggt tcttgaccgc gcgcaagatg cggaactggt ggccgctcag accctcgaag
     5761 gacagcacgc tgtccaccat gtgctcgatg accttgggac cggcgatctg cccgtccttg
     5821 gtgacatggc cgatgaggat gagggccgag ccgctcgcct tggcgtggcc gatcaaggca
     5881 gacgcgcaac ccctgagctg ggagatggtg ccgggtgcgg cttccacgcc ctccgaccac
     5941 agcgtctgca ccgagtcgat gacgatgagg gcagggggtg tcccgcgcgc caaggtcgct
     6001 acgatgttgg cgaggttggt ctcggctccg agggcgacag gcgcggcgga gagcccaagg
     6061 cgcgccgccc gcatgcgaat ctgcgccggc gcctcctcgc cggaaagata gacgacagcc
     6121 gcacccgacg cggcaagcgc ggccgccgcc tgaagcagca aggtcgattt gccgatgccg
     6181 ggatcgccgg cgacgaggac cgccgagccc ggcacgaggc cgccgccgag gacccggtcg
     6241 aactccgcga taccagtcgg aaggcgcggc gcttcgcttg tcgccgcgga aagggtctca
     6301 agcgccgcaa gccgcccctt gccgcccttg atggcgatca gtccgggcga ccctgtgggc
     6361 gcccgctcct cgatgatcga attccactcg ccgcaggagg cgcatttgcc ggaccagcgg
     6421 ttgctgaccg cgccgcaggc ttggcagacg aacgcgcgcg aggctttggc catggcttgg
     6481 tttagcctcc gacatagcgc cggaaggcgc gctggccgag acgcgtgagc acctcgtaat
     6541 cgatggtgcc ggcatgcgct gcgagcacat gagccggaac attcggtccg atcagctcga
     6601 cccaagcgcc gcgtcgcgcg agctcttccg gcacatggct cacgtcgagc gcaacgagat
     6661 ccatcgagac gcggcccacg atcggcgcgg catgaggccc gaggtaacca gaaaaacctt
     6721 gctcgccgtc ggcgatagac agcgctcggg cgaagccgtc ggcatagccc acagaaacaa
     6781 cggcgagccg cgcagcctcg cggatcgtgc gggtcgcgcc atagcccacg gtttctcccc
     6841 gcgggacgtc gcgcacctgc atgatgcggc ccttgagttg caccacgggc cggaacgggt
     6901 tgtggccgtg accgcgggga tggccgccgt aaatcgcaat acccggcctg accacatcat
     6961 agaaaaaggc gcggccaagc aggatgccgg gggaattggc gagactcgaa ggcgctttgg
     7021 ggagctgcgc gcgaaggcgg tcgaacatgc ttcgctgcgc gccgtttttg ggatgctccg
     7081 gctcgtcggc gcaggcgaga tggctcatca ccaaagatag agtcagcgcg gaccaaagtt
     7141 cagctgctct cgcgacctgg tccacatctt ccgccgagag accgaggcga ttcatgcccg
     7201 tgtcgatatg gacggcgcag tcaagccgtt cgccttgcgt ctcgcagaac gccgcccact
     7261 ctctcacctc atcggcgctg ttcaacacgg gtctgagatg atgggcgcgg tagagctcgg
     7321 ccgtgccccg catcagtccg gcgagcacat agatgatggc atcaggaagg agcgcgcgca
     7381 gtcgctctcc ctcgtcgagg gtggcgacga agaaggtctt gcagccgatg cgtgagagcg
     7441 tggaggaaat cggaaccatt ccgagcccat aggcgtcggc cttgaccacg gcggcgcatt
     7501 cggccgggct cgcgaggtcg cgcaaagtgc ggtaattggc ggcgaccgca tcgagatcga
     7561 tggtgaggat tgcggtctcg cccggccctt cgtctcgcgc gcccgagggg acctcgcccg
     7621 cgcgcatcgc tctcacgcgc ggcgctccgg caggcgctcc tccgccgcga gattgccgaa
     7681 ccgcgtgaat tccggcgtga attgcaggtg gaccgtcccg gttggtccgt ggcgctgctt
     7741 gccgatgatg atctcggccc tgccgctcac cgtctccatc tctgtctgcc actgcttgtg
     7801 ct
//
LOCUS       DASUQP010000245         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_532025,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000245 DASUQP010000000
VERSION     DASUQP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532025"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..808
                     /gene="pdxH"
                     /locus_tag="VFY74_11340"
     CDS             194..808
                     /gene="pdxH"
                     /locus_tag="VFY74_11340"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX5932322.1"
                     /translation="MRDPLVGDFTEAADPLVLFHAWMAEAEAAEPVDPEAFALATVDA
                     SGLPNVRMILLKGADERGLVFYTNCESAKGGELAANPKAALLFYWKSLGRQIRVRGPV
                     EPASDAEADAYFATRHRESQIGACASRQSRPLASRAALEAQVARLAETFAGGEVRRPG
                     YWRGYRLEPLEIEFWKSGSFRLHDRIVFRRTSPQAAWTKTRLYP"
     gene            924..1817
                     /locus_tag="VFY74_11345"
     CDS             924..1817
                     /locus_tag="VFY74_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX5932323.1"
                     /translation="MARYATARYAVKEVHEATWARRIALFFVQLLILTVLLHRFTSLT
                     TPVALNLIGVSLAGLFIAIAIAIFALVRIWFGGQTGAAQAFAAIFIACLGLAVPAFYL
                     SKTVLLPRLNDVATSPDEPLAFKTLLSQRPVDANPIKDPDAAAVEAQVEAYPDLGPMV
                     LERTAPEVFSLVQEAVERQGWTIASSETPGETGIGRIEATDQSLVMGFTDDVVVQVKG
                     DDAHAVVDVRSVSRYGLHDLGANAARIRALFEEVTTALEKGEKTVLEQASPKEEAVPD
                     KKDATKKRVKKRAKKRRSTGR"
     gene            complement(1777..2688)
                     /locus_tag="VFY74_11350"
     CDS             complement(1777..2688)
                     /locus_tag="VFY74_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5932324.1"
                     /translation="MSAELAFNRNQDVDYGVAQEVGPGIRRIVANNPGPYTFLGTNSY
                     VVGHGEVAVIDPGPADEQHLRAIAAAIKGERLAYILVTHSHRDHCQGARGLQALAGGR
                     IAAFGPTGTPRGAGAPGLGDAFVDPCFVPDRKLADGDTVRGKGFALDVLHLPGHAPDH
                     LCFALVGKRTVFTGDHVMGWNTTVIAPPEGNMAQFLASLERLMQRHDKMFLPGHGGRI
                     QTPQRVVKAYMMHRKWREQTILACLDEGVRTVPLIVERLYGTLDAGLKEAAALSVFAH
                     LEHLAARELVASDGPSGLSSVFSSRAS"
     gene            3594..>3859
                     /locus_tag="VFY74_11355"
     CDS             3594..>3859
                     /locus_tag="VFY74_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932325.1"
                     /translation="MNATHNPHAAAHQARDAYRNVTAQLGLLGLNPAIPEGVRSLAEK
                     TVSQTRQVYDRSSDALDAAVATFEQTFDAAGQNAAAFNRKIIDIS"
BASE COUNT          701 a         1224 c         1189 g          745 t
ORIGIN      
        1 ccgctgcgcg actgtagcat ccttcctcat attaggcaaa tgttggccaa ctactatgaa
       61 gtcaaagaag aaatggcgta ttttagcgtt atttttgact tgctatcgct gtgagcaaaa
      121 cgtaaatgcc cgtgccgcgc caggatcgaa ccgcaagcct cgtcgccgcg ggaagggccc
      181 gagcggctga gccatgcgcg atcctttggt gggagacttt accgaagctg ccgaccccct
      241 tgtcttgttc cacgcttgga tggcggaggc ggaagcggcc gagcccgtgg accccgaggc
      301 gtttgccctt gccacggtcg atgcctcggg cctgccgaat gtgcggatga ttctgctcaa
      361 gggggccgac gagcgcggtc ttgtcttcta taccaattgc gagagcgcca agggcgggga
      421 gcttgccgcc aatcccaagg cggcgcttct cttctattgg aaaagccttg gacggcagat
      481 ccgcgtgcgc gggccggtcg agcccgcgag cgatgcagag gccgacgcct atttcgccac
      541 tcgccatcgc gagagccaga tcggcgcctg tgcctcacgc cagtcgcgcc cgcttgcgag
      601 ccgcgcggcg ctagaggctc aagtggcgcg gctcgccgag acctttgccg gtggcgaggt
      661 gcgacgtcca ggctattggc ggggctatcg gcttgagccg ctcgaaatcg agttctggaa
      721 gagcgggtcg ttccgcctgc atgaccgaat tgtgttccgg cgcacctccc cgcaagcggc
      781 ctggacaaag acacgcctct atccctaggg cggaatcgac cacaaacctg ccacgtgggc
      841 gcgatagcta gtataagcga gggtcggggc gcgtccccgg cgagggacaa cgagcttttg
      901 agggccgcga attgagcttc tcgatggcgc gctatgcgac ggcacggtac gccgtcaagg
      961 aagtgcacga ggccacttgg gcgcggcgca tcgccctctt cttcgttcag ctcctgattc
     1021 tgacggtgct tctgcaccgc ttcacgtcgt tgacgacgcc ggttgcgctg aacttgatcg
     1081 gtgtgtcgct tgccgggctc ttcatcgcca tcgcgatcgc catctttgcc ctcgttcgca
     1141 tctggttcgg cggacagaca ggcgcagccc aggccttcgc ggcaatcttt atcgcctgcc
     1201 ttggccttgc cgtgcccgcc ttttatctct cgaaaaccgt tctactgccg cgcttgaacg
     1261 atgtcgcgac gagccccgac gagccgctcg cgttcaagac gctcctcagc cagcgccctg
     1321 tcgacgccaa cccgatcaag gaccccgacg ctgccgccgt cgaagcgcag gtcgaggcct
     1381 atcccgatct cggaccgatg gtgctggagc gaaccgcgcc ggaggtgttc agcctggtgc
     1441 aggaggcggt ggagcggcaa ggctggacca tcgcgtcgag cgagacgccc ggggaaaccg
     1501 gcatcggccg catcgaggcg acagaccaga gcttggtcat gggcttcacc gacgatgtcg
     1561 tcgtgcaggt caagggcgac gacgcccacg ccgtggtcga cgtccgttcg gtatcgcgct
     1621 atggcctgca cgatctcggc gccaatgctg cgcgtatccg cgcgctgttc gaggaagtga
     1681 ccacggcgct ggagaagggc gaaaagaccg tgctcgagca ggcctcgccc aaggaagagg
     1741 ctgtccccga caagaaggat gctacgaaga agcgagtcaa gaagcgcgcg aagaaaagac
     1801 gctcgacagg ccgctaggcc cgtccgacgc gaccagctcg cgggcggcaa gatgctcgag
     1861 atgcgcgaac accgagagcg cggccgcctc ttttagcccg gcgtcgagcg tcccgtacag
     1921 gcgctcgacg atgaggggga cggtgcgcac cccctcgtcg aggcaggcga ggatggtttg
     1981 ttcgcgccac ttccgatgca tcatataggc cttcaccacc cgttgcggcg tctggatgcg
     2041 tccgccgtga cccggcagaa acattttgtc atgccgttgc atcaaccgct cgagcgaggc
     2101 gaggaattgc gccatgttgc cctcgggagg cgcgatgacc gtcgtgttcc agcccatcac
     2161 atgatcgccg gtgaacacgg tgcgcttgcc gacgagtgcg aagcaaagat gatcgggtgc
     2221 gtggcccggc agatgcagaa cgtcgagcgc gaaccccttc cccctgaccg tgtccccgtc
     2281 ggcaagtttc cggtccggca cgaagcacgg atcgacgaag gcatcgccga ggcccggtgc
     2341 gcccgcgccg cgcggcgttc ccgtcggccc gaaggcagcg atccttccgc ccgcgagcgc
     2401 ctgcaagccg cgcgcgccct ggcaatggtc acgatggctg tgcgtgacca gaatgtaggc
     2461 cagccgctcg cccttgatcg ctgcggcgat cgccctcaga tgctgctcgt cggcgggacc
     2521 cggatcgatg acggcgacct caccgtgccc cacgacatag ctgttggttc cgagaaacgt
     2581 gtaaggtccc ggattgttgg ccacgatgcg ccggatgccg ggcccgacct cctgggccac
     2641 gccataatcg acatcctggt tgcggttgaa ggcaagctcc gccgacatga tcttctttgg
     2701 ctgttggtct tctcgtcgaa cgcctattct agcggagcgc atcagcggcg gaagcgccgc
     2761 ccgtgtcgca ccgttaactt tcccatcgca atttgcgcaa aatgtggccc gcgacaatga
     2821 cttgagtgtc gcccgcgccc ctctagctcc tgtcggggct acacgaatgc ccgcgccagc
     2881 atgccaggct tggccgcgct gtttggaaag cgcagccgat gccgttagca ggagatgcgg
     2941 tctcagtagt atcgcgaata gcgggcgcac cggtagtagc ggtcccacca gtagtcactg
     3001 ccggtggaaa gggctttgcg atagatctgt gcgctcttgg ggggcattcg gcttcgcatg
     3061 catagccaac ggccttcgcg gctagagcct tcttggcatc taaacggatt tcttccaact
     3121 cccggtgatt gcatcgttca taaaatcatc gatttcagct tcggtcatcg ggtgcgctga
     3181 gattgcaaga tccggagccc gactgagggc gctcgacttc acgtagatct gcacgtaggc
     3241 agcggtagga ttaggttcgt gtttgccgcg acctcgatga aagtcatttt aggcgcgcat
     3301 gtgtctccta ctttggtctc agagcagagc ttcttgcacg tgcaacttgt gcgggcaatc
     3361 gtaatgatct gtgcgcagcg cttgtaatgc gaccgttcaa gccccatatc tctactactg
     3421 gaacgccgaa ccctacgcgc tgacggtgaa gactaccgtg caactttgtg cacgccccct
     3481 ttcccaaatc agcacccagc gctccttaaa cggagagccg ctttttgtta aacggagagc
     3541 cgctttttgg agcgtggtcg agcggttgcc agcccgcgaa ccgacagtta ctcatgaacg
     3601 ccacccacaa tccccacgct gccgcccacc aggcccgcga tgcgtatcgc aatgtgacgg
     3661 cccaactcgg tctcctcggt ctcaaccctg ccattccaga gggcgtacgt tcccttgccg
     3721 agaagactgt gtcgcagact cgtcaagtct atgaccgctc ttcggatgcc ctcgacgcgg
     3781 ccgtggcgac gtttgagcag accttcgacg ctgccggcca aaatgctgca gccttcaacc
     3841 gcaaaattat cgatatctc
//
LOCUS       DASUQP010000246         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1757896, whole genome shotgun sequence.
ACCESSION   DASUQP010000246 DASUQP010000000
VERSION     DASUQP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757896"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFY74_11360"
     CDS             <1..688
                     /locus_tag="VFY74_11360"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5932326.1"
                     /translation="IFTGTSYISFLWSVVLAVATVVLLALICYPIAYGLVHVFGRWST
                     LLTLLFTIPLFVSENIRLYGWVLFFIKNGVLLGTMKSLFGIEMGSILFTLGAILLGMV
                     YVYLPFMLFPMTLGVAMVPNDAREAARDLGASRWQVFREVELPLSMPGIVIGCLLTFV
                     LAVGAIAEAKILGGQRIIPITHDIEIAFTYSQNWPLGAALAVLLMLLVGVLVISALRR
                     FDLDQILGRR"
     gene            694..1551
                     /locus_tag="VFY74_11365"
     CDS             694..1551
                     /locus_tag="VFY74_11365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5932327.1"
                     /translation="MLAFPERVRHGLITVWTVAVLVFVFLPGVTILLASLTASRYFTF
                     PIPKWGFDWWDKTFSSFEVRQLLATSLSIAAVVMVIAVAIGFLGALAFARYDWRGRSL
                     YQKIVLLPIFFPQTVLGLAMLLWFNAIGLELSWKTAVFAHLVWIVPVVTLVMAIQVYS
                     FDPALEEAAFDLGASRWQVLREVTLPVLFPGIFSGALFAFLLSWGNFPLSLFTTGADT
                     TVPEYLYAKMVAGYTPGVPVLGTVSTIGATLILLGGYVLILIRGRKRELAVEATGRRE
                     LAAEVAASP"
     gene            1585..2364
                     /gene="fabG"
                     /locus_tag="VFY74_11370"
     CDS             1585..2364
                     /gene="fabG"
                     /locus_tag="VFY74_11370"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX5932328.1"
                     /translation="MLTSIAGRSVIVTGSSKGIGKGIAKVFAKNGGKVLVVSRHLDEA
                     EACADELRRAGGTLKGHAADVTKLADMQGMADAAAKAHGGLDILCANAGIFPQTKIED
                     LSPEDWDHVMATNLKGTFLAIKACLPHLKKSDQGRVVITSSITGPVTGFPGWAHYGAT
                     KAGQLGFMRTVSIELAKYGVTVNAVMPGNILTEGLIALGPEYERSMAESIPLKKLGTV
                     EDIGYAALFLASKEAAYITGQTIVVDGGQILPESLDALAQA"
     gene            complement(2361..>3000)
                     /locus_tag="VFY74_11375"
     CDS             complement(2361..>3000)
                     /locus_tag="VFY74_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5932329.1"
                     /translation="TDAAGFETLGEITARMHAHVRNWQRPPWFERHTWDFETSLGSRP
                     HWGRWQDGMGVSAEALETMGETVRLIDRRLDRFGKRPDRFNLIHGDMRLANLLMDTGT
                     IKVIDFDDCGFSWFLYDCATTVSFFEDSPEVPSLIDAWVRGYRRAGTLSAEDEAEIDT
                     FVMLRRLLLVAWIGSHSETELAQSMGVAYTQATLPLCERYLSRFGEARGRQA"
BASE COUNT          483 a          983 c          924 g          610 t
ORIGIN      
        1 gatcttcacc gggacctctt atatctcctt cctttggtcg gtggtgctcg cggtcgcaac
       61 cgtcgtcctg cttgcgctca tctgctaccc catcgcctat ggcctcgtgc acgtgttcgg
      121 ccgctggtct accctcctca cgctgctctt caccattccc ctcttcgtct cggagaacat
      181 ccgcctctat ggctgggtgc ttttcttcat caagaacggc gtacttctcg gcaccatgaa
      241 atcgctgttc ggcatcgaga tgggtagcat cctgttcacg ctcggcgcga tccttctcgg
      301 catggtctac gtctacctgc cgttcatgct gtttcccatg acgctcggcg tcgccatggt
      361 gccgaacgat gcgcgcgagg cggcacgcga tctcggcgca agccgctggc aggtgtttcg
      421 tgaggtcgag ctgccgcttt cgatgcccgg catcgtgatc gggtgcctgc tcacgttcgt
      481 gctcgcggtc ggcgccatcg cggaagccaa gattctcggc ggccagcgca tcatcccgat
      541 cacgcatgac atcgagatcg ccttcaccta ttcgcagaac tggccgctcg gcgcggcgct
      601 tgccgtcctt ctcatgctac tggtcggcgt gctcgtcatc tccgccctgc gccgcttcga
      661 cctcgatcaa atcctgggga ggcgctagtc ccaatgctcg cttttccgga gcgtgttcgc
      721 cacgggctta tcacggtgtg gacggtggcc gtcctcgtgt tcgtgttcct cccgggcgtc
      781 acgatcctgc tcgcctcgct cacggcgagc cgctatttca ccttcccgat ccccaagtgg
      841 ggcttcgact ggtgggacaa gacgttctcc tccttcgagg tgcgccagct gcttgcgacg
      901 tcgctatcga tcgctgccgt ggtcatggtc atcgccgtgg cgatcggctt cctgggcgcg
      961 ctcgcctttg cccgctatga ctggcgcgga cgctcgctct accagaagat cgtgctcctg
     1021 ccgatcttct ttccgcagac cgtgcttggc cttgcgatgc tgctctggtt caacgcgatc
     1081 gggctcgagc tatcttggaa gaccgcggtc ttcgcccacc tcgtctggat cgttccggtc
     1141 gtcaccctcg tcatggccat ccaggtctat tcgttcgacc cggcgctcga ggaagcggcg
     1201 ttcgacctcg gggcgtcgcg ctggcaggtg ctccgcgaag tgacgctccc ggtgctcttt
     1261 cccggcatct tttccggcgc gttgttcgcg ttcctgttgt cgtggggcaa cttcccgctg
     1321 tcgctcttca cgaccggcgc cgatacgacg gtgcctgagt acctctatgc caagatggtc
     1381 gccggctata cgccgggcgt cccggtgctc ggcacggtct cgaccatcgg cgcgaccctc
     1441 atcctgctgg gcggttacgt gctgatcctg atcaggggac gcaagcgcga gcttgccgtc
     1501 gaggcgacgg gcaggcgaga gctcgcagcc gaggtcgcgg ctagtccata gaggcggtgt
     1561 tcacctaatg ttgcggagag aaaaatgctg acatcgatcg caggccggtc cgtcatcgtc
     1621 acgggatcga gcaaaggtat cggcaagggt atcgccaagg tctttgccaa gaacggcggc
     1681 aaggtgcttg tcgtgtcccg acatctcgac gaggccgagg cttgcgccga cgagctgcgc
     1741 cgcgccggcg gtacgttgaa gggccacgcc gccgacgtga cgaagctcgc agacatgcaa
     1801 ggcatggctg acgcggccgc gaaagcgcac gggggcctcg acatcctgtg cgccaatgcc
     1861 ggtattttcc cgcagaccaa gatcgaggac ctctcgccgg aagactggga ccacgtcatg
     1921 gcgacaaacc tcaaggggac ctttcttgcg atcaaggcct gcctgccgca cctcaagaag
     1981 tcggaccagg gccgcgtcgt gataacgtca tcgatcacag gtccggtcac gggctttccg
     2041 ggatgggccc attacggcgc gacgaaggcg ggtcagctcg gcttcatgcg cacggtctcg
     2101 atcgagctcg ccaagtatgg cgttaccgtc aatgcggtca tgcccggcaa tatcctgacc
     2161 gaaggattga tcgcgctcgg gccggaatac gagcgctcga tggcggaatc aattccgttg
     2221 aagaagctcg ggacggtcga ggatatcggc tatgccgccc tgttcctcgc ctccaaggag
     2281 gccgcctaca tcacgggtca gacgatcgtc gtcgacggcg gccagattct gcctgagagc
     2341 ctggacgcac tcgcgcaagc ctaggcctgc cgcccgcgcg cttcgccgaa acgcgacaga
     2401 tagcgctcgc acaagggaag cgtggcttgc gtgtatgcga cgcccatgga ttgagcgagc
     2461 tccgtctccg aatgcgagcc gatccaagcg acgagaagaa gacggcgcag catcacgaac
     2521 gtgtcgattt cggcctcgtc ctcggctgag agcgtgccgg cgcgccgata gccccgcacc
     2581 cacgcgtcga tgagcgacgg cacctcggga ctgtcctcga aaaaggacac cgtcgtcgcg
     2641 cagtcataga ggaaccagga aaagccgcag tcgtcgaaat cgatgacctt gatcgtgccg
     2701 gtgtccatga gcaggttggc aagacgcatg tcgccgtgga tgaggttgaa ccggtctggc
     2761 ctcttgccga agcggtcaag gcgccggtcg ataagccgga ccgtctcacc catcgtttcg
     2821 agggcctcgg cagacacgcc catgccgtcc tgccaccggc cccaatgcgg ccgcgacccg
     2881 agactcgttt cgaagtccca ggtatggcgc tcgaaccagg gcggccgctg ccagttgcgc
     2941 acatgggcgt gcatgcgggc cgtgatctcg cccaaggtct cgaagccggc ggcgtccgtc
//
LOCUS       DASUQP010000247         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_533337,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000247 DASUQP010000000
VERSION     DASUQP010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533337"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VFY74_11380"
     CDS             complement(<1..750)
                     /locus_tag="VFY74_11380"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX5932330.1"
                     /translation="MANVVVVGSQWGDEGKGKIVDWLSERADIVVRFQGGHNAGHTLV
                     VDGTTYKLSLLPSGVVRKGKLSVIGNGVVIDPWALAEEIAKIEKLGVKISRDNLRIAE
                     NATLILPLHRELDSLREAEAGDGRIGTTKRGIGPAYEDKVGRRAIRVMDLQDLPSLKP
                     KIERILAHHNPLRRGLGQPEISADELCAQLTEIAPKILPFVDTVWALLDAKRRQGKRI
                     LFEGAQGALLDIDHGTYPFVTSSNTVAAQAAT"
     gene            complement(888..2063)
                     /locus_tag="VFY74_11385"
     CDS             complement(888..2063)
                     /locus_tag="VFY74_11385"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX5932331.1"
                     /translation="MSTLVKPTARPNNPRFSSGPCTKHPGWSVDRLEGALVGRYHRGP
                     ETKARLALAIERTAALLELPADYRVAIVPASDTGAVEMALWSLLGPRGVDVLAWEAFS
                     RDWVTDIVEQLKVQNVRALLAPYGELPDLGQVDCDRDVVFAWNGTTSGVRVPNADWIR
                     SDRAGVTICDATSAVFAQRIDWAKLDAATFSWQKVLGGEAQHGMLILSPRAIERLETY
                     TPPWPLPKLFRMTAGGKLIEALFEGSTINTPSMLCLQDYLDALDFVESVGGLEGTITR
                     SDANAAVLSDWATKRPWVDFLARVPETRSNTSVCLKIVDPEVAVLPPEMRDAVPGAIA
                     ALLDAEDAARDIGSHRAAPPGLRIWTGATVERSDVEALLPWLDWAFAEVKRAVLKAA"
     gene            complement(2114..3463)
                     /gene="glmM"
                     /locus_tag="VFY74_11390"
     CDS             complement(2114..3463)
                     /gene="glmM"
                     /locus_tag="VFY74_11390"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5932332.1"
                     /translation="MPRKFFGTDGIRGSANAYPMTSEVALKVGMAAGRLFTKGSYRHR
                     VVIGKDTRLSGYMIESALVSGFTSVGMDVFQLGPMPTPAVAMLTRSLRADLGVMISAS
                     HNPFGDNGIKLFGPDGYKLSDENEEEIERLMEGDGADLLADSEKIGRATRIDSARERY
                     IEFAKRTLPKNIRFDGVRAVIDCANGAAYKVAPEALWELGAEVIRIGVDPNGLNINKD
                     CGSTAPDDLIRKVNEVRADIGIALDGDADRVVIVDEKGHIIDGDQLMAVIAESWQKRG
                     RLAGGGIVATVMSNLGLERYLESLDLGLARTPVGDRYVVEHMRQHGYNVGGEQSGHIV
                     LSDFSTTGDGLVTALQLLSVVVQSGRPVSELCNRFEPLPQILKSVRYKNGGQPLDNVA
                     VRRAIDSGKSKLGKSGRLVIRPSGTEPVIRVMAEGDDEKLVKTVVSEIVHALTDAAA"
     gene            complement(3618..>3874)
                     /locus_tag="VFY74_11395"
     CDS             complement(3618..>3874)
                     /locus_tag="VFY74_11395"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5932333.1"
                     /translation="HNLALLSHTTLFHGLGVPLLSGASRKRFIQGLSGGAEPRRRDPG
                     SHAAAIAAAAQGAQILRVHDVEGTRQALGVWRASVFGTEN"
BASE COUNT          671 a         1255 c         1271 g          677 t
ORIGIN      
        1 ggtcgcggcc tgagccgcga ccgtgttcga cgacgtaacg aagggatagg tcccgtgatc
       61 gatgtcgagc agagcgcctt gggcgccctc gaagaggatg cgcttgccct gccgccgttt
      121 ggcgtcgagc agcgcccata ccgtatcgac aaaggggagg atcttgggcg cgatttcggt
      181 gagctgcgcg cagagttcgt cggcggaaat ttcaggctgg cccaggcccc ttcggagcgg
      241 attatgatgg gcgaggatgc gctcgatctt gggcttgagc gacgggaggt cctgaaggtc
      301 catcaccctg atggcccgac gtccgacctt gtcctcatag gccgggccga tgccgcgctt
      361 ggtcgtgcct atgcgcccgt ctcccgcctc ggcctcgcgt agcgaatcga gctcgcggtg
      421 gagcggcagg atgagcgtcg cattctcggc gatgcggaga ttgtcgcggg aaattttgac
      481 cccgagcttc tcgatctttg cgatctcctc ggccaacgcc caggggtcga tcaccacgcc
      541 attgccgatg accgagagct tgcccttgcg caccacgccc gacggcagca gcgagagctt
      601 gtaggtcgtg ccatcgacga ccagtgtatg gccggcatta tggccgccct ggaagcgcac
      661 gacgatatcg gcgcgctcgg acagccaatc gacgatcttg cccttgccct cgtcgcccca
      721 ttgggagccg acgaccacga cattggccac gtgaacagtc cttcgattcg aggccgggga
      781 cgcagaaacg gccccgcttt caggtggggc cgtgtcacct tatagcgcgc tcgcctcccc
      841 gatgcgagcc tgctgatccc tcgcctcgag gcgcgggaaa gcgcgggtta agccgccttc
      901 agcaccgccc gtttcacctc ggcgaaggcc cagtcgagcc agggcaaaag cgcctcgacg
      961 tcgctccgct cgaccgtggc cccggtccag atgcgcaagc ccgggggcgc ggcgcggtgg
     1021 gaaccaatgt ccctggcggc gtcctcggcg tcgagcaacg cggcgattgc tccgggcacg
     1081 gcgtcgcgca tctctggcgg gagcactgcg acctcgggat cgacaatctt aaggcagacc
     1141 gaagtgttcg agcgcgtctc agggacccgc gcgagaaaat cgacccaagg cctctttgtc
     1201 gcccaatcgg aaaggaccgc ggcattggca tctgagcgcg ttatcgtgcc ttcaaggccg
     1261 cccacgcttt cgacgaagtc gagcgcatcg agataatcct gaaggcacag catcgacggc
     1321 gtgttgatgg tcgagccttc gaacagcgcc tcgatcagct tgccgcctgc ggtcatgcgg
     1381 aacagcttcg gcagaggcca gggcggcgtg taggtttcga ggcgctcgat cgcccggggc
     1441 gagaggatca gcatgccgtg ctgggcctcc ccgccaagca ccttctgcca ggagaaggtc
     1501 gcggcatcga gcttcgccca atcgatcctt tgggcgaaca ccgcagaggt tgcatcgcag
     1561 atcgtcacgc ccgcacgatc gcttctgatc caatcggcat tggggacgcg cacgccggac
     1621 gtggtgccgt tccaggcgaa caccacgtcg cggtcgcaat cgacttggcc gagatcgggc
     1681 agctcgccgt aaggcgcaag gagcgcgcgg acgttctgga ccttaagctg ttcgacgata
     1741 tcggtgaccc agtcgcggct gaatgcctcc caggcgagca catcgacgcc gcgcggcccg
     1801 agcagagacc acagcgccat ctcgaccgcg ccggtatcgg aagcgggcac gatcgccacg
     1861 cggtaatcgg cgggaagctc aagcagtgcg gccgtgcgtt cgatcgcaag cgcgagcctc
     1921 gctttggtct cgggcccacg atgatagcgc ccgacgagcg cgccctcgag gcgatccacc
     1981 gaccagccgg gatgcttggt gcaggggccc gaggagaagc gcgggttgtt cggccgcgcc
     2041 gtgggtttca caagcgtgct cacagttcgc atcgacacat tgaaacgatt gttcccgatc
     2101 ccgcggcgcg gatctaggcg gcggcgtcgg tgagcgcgtg gacgatctcg gagacgaccg
     2161 tcttgacgag tttctcgtca tcgccctcgg ccatgacccg gatcacgggc tcggtgcccg
     2221 acggacggat cacgaggcgg cctgatttac cgagcttcga cttgccgctg tcgatggcgc
     2281 gtcgcaccgc cacattgtcg agcggctggc cgccattctt gtagcggacg ctcttcagga
     2341 tttgcggcaa cggctcgaag cgattgcaga gctcgctgac cggcctcccg ctctgcacca
     2401 ccacggacag aagttgcagc gcggtgacga gcccgtcgcc cgtggtcgag aaatccgaca
     2461 gaacgatatg gcccgactgc tcgccgccga cattgtagcc atgctggcgc atatgctcga
     2521 ccacgtagcg gtcgccaacc ggggtgcgcg cgagcccgag atcgagactc tcaagatagc
     2581 gctcgagccc gagattcgac atgaccgtgg cgacgatgcc gccgcccgcg agacgcccgc
     2641 gcttctgcca cgactcggcg atgacggcca tgagctgatc gccgtcgatg atgtggccct
     2701 tctcgtcgac gatcaccacg cgatccgcat cgccatcgag cgcgataccg atatccgcgc
     2761 gcacctcgtt gaccttgcgg atcagatcgt ctggcgccgt cgatccgcaa tccttgttga
     2821 tgttgaggcc gttgggatcg acgccgatcc tgatgacctc ggcgcccagc tcccacaacg
     2881 cctccggcgc caccttgtag gcggccccat tggcgcaatc gattaccgcc ctgacgccgt
     2941 cgaagcggat gttcttcggc agcgtgcgct tggcgaattc gatatagcgc tcccgcgcgc
     3001 tgtcgatgcg ggtcgcgcgg ccgatcttct cggaatcggc gagcagatcg gcgccgtcgc
     3061 cctccatcaa ccgctcgatc tcttcctcgt tctcgtccga cagcttgtag ccgtcgggcc
     3121 cgaacagctt gatgccgttg tcgccgaaag gattgtgaga cgctgagatc atcacgccga
     3181 gatcggcgcg gagcgaacgg gtcagcatgg cgacggcagg cgtcggcatc gggccgagct
     3241 ggaacacgtc catgccgacc gaggtgaagc cggaaacgag cgcggactcg atcatgtagc
     3301 cggagagccg cgtgtccttg ccgatcacga cgcggtggcg gtaggagccc ttggtgaaca
     3361 gcctgccggc ggccatgcca actttgagcg ccacctccga ggtcatcgga taggcgttgg
     3421 cgctgccacg gatgccatcc gtgccaaaga atttgcgagg cattcggctg cccccccgtg
     3481 tgttcccggt cgctgcctgg ggcttgttat ggtctgaccg gcgcatggga ttatgcccct
     3541 ggtctaccac ccttgagttc aaaaaatgta agcaaaagtt acaccagggc ccggcgcagc
     3601 ggagttaccg atttggctta attttcagtg ccaaagacgg aagcacgcca tacgccgagc
     3661 gcctggcggg tgccttccac gtcgtggaca cgcagaattt gcgccccttg agcggcagcg
     3721 gcgatggccg cggcgtggga acccgggtcg cggcggcggg gttcggctcc gcccgacagc
     3781 ccctggatga accgcttgcg cgaagccccg ctcaggagcg gcacgccgag cccgtggaag
     3841 agggtcgtat ggctgaggag ggcgaggttg tggg
//
LOCUS       DASUQP010000248         6252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_396663,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000248 DASUQP010000000
VERSION     DASUQP010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396663"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFY74_11400"
     CDS             complement(<1..247)
                     /locus_tag="VFY74_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932334.1"
                     /translation="MTHRVRVHLVLVAWLLLAALGGVARAAESLIWTEKASDGFVSLS
                     YGPVDPAKTPVFFLSCFDAMGIAVLDLRQEIEGAKAGD"
     gene            complement(294..719)
                     /locus_tag="VFY74_11405"
     CDS             complement(294..719)
                     /locus_tag="VFY74_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX5932335.1"
                     /translation="MLTVSTYLAPSAIDGIGVFAGEYIERGRLIWSLNPKFDIFIHPS
                     EIGAYPPFLQDFVARYTYPHLEIPGVVILDSDNGRFMNHSLSPNTDFCIFDKGYALFD
                     VAQGEELTCNYHEFDPNFTGYFPTVQVVMAESRLHAGSL"
     gene            complement(760..1335)
                     /locus_tag="VFY74_11410"
     CDS             complement(760..1335)
                     /locus_tag="VFY74_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX5932336.1"
                     /translation="MSQGSTAYLTDKCEVQNRDVTGGKAVYARNIIEPGELIGVWSGR
                     IISAETLDELPEPIRRHTVQVEEGLYLASHSQAEPPDFINHSCEPNAGLNGQIAIVAL
                     NRIHPGEEVTIDYAMCDGSPYDEFECACGSAACRGRVTGEDWRNPELWERYAGHFSPY
                     LERRIRALKRRRFRRKRSLVAVGTEAAESTA"
     gene            1463..2212
                     /locus_tag="VFY74_11415"
     CDS             1463..2212
                     /locus_tag="VFY74_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX5932337.1"
                     /translation="MPIYKKTRLDQLLVDSGLAPTRSRAADLIRRGCVSVAGKPQLKP
                     GALVAPGASFAVSPETCAYVSRGGLKLAAALDAFGFDPRGRVALDLGASTGGFTEVLI
                     GRGAAKVYAVDVGKGQLHAKLRANPRVIALEGTDARALDKSVIGEEVTAIAADVSFIG
                     LALALPAALRLAALGAWLVALIKPQFEAGREAVGKGGIVRSEADRRKAVAKIRKFIEE
                     QPGWTVIGEIPSPIRGGSGNEEFLIGARHGA"
     gene            complement(2218..3324)
                     /locus_tag="VFY74_11420"
                     /pseudo
     CDS             complement(2218..3324)
                     /locus_tag="VFY74_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
     gene            complement(3628..4329)
                     /locus_tag="VFY74_11425"
     CDS             complement(3628..4329)
                     /locus_tag="VFY74_11425"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX5932338.1"
                     /translation="MRFEHIVVLTGAGLSAESGLGTFRDKGGLWEEFDLEAVATPQGF
                     ARNPARVHDFYNLRRRRLGSVQPNAAHLALARLEREYRGNVLTVTQNIDPLHEAAGTE
                     NLIHMHGELARVLCAACETSVGWTHDLSTGTVCGSCGKAGTMRPDVVWFGEMPREMDR
                     IYAALTACDLFISIGTSGSVYPAAGFVTEARAAGAHTVELNLEPSDGASLFTEAHYGP
                     ATEIVPSFVERLLRA"
     gene            complement(4522..5613)
                     /gene="aroC"
                     /locus_tag="VFY74_11430"
     CDS             complement(4522..5613)
                     /gene="aroC"
                     /locus_tag="VFY74_11430"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5932339.1"
                     /translation="MSFNSFGHLFRITTWGESHGPAIGCVVDGCPPGISITEEEIQAY
                     LDKRRPGQSRYTTQRQEPDRVRILSGVFTDESGAQVTTGTPIALEIENVDARPKDYGD
                     IKDKFRPGHADYTYLMKYGLRDWRGSGRASARETAARVAAGAIARKVVPGMVVRGALV
                     QIGPHAIDRRNFDWNEVGNNPFFSPDAKAAEVWAGFLDQVRKKGSSAGAIIEIVAAGV
                     PPGLGAPVYGKLDQDIAAGLMSINAVKGVEIGAGFVSAALSGEENADEMRMRNDGTPE
                     FLSNNAGGILGGISTGQPIAARFAVKPTSSILTPRRTVDAKGHDTEISTKGRHDPCVG
                     IRAVPIGEAMVAIVLADHYLRHRGQTGKG"
     gene            5856..6149
                     /locus_tag="VFY74_11435"
     CDS             5856..6149
                     /locus_tag="VFY74_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5932340.1"
                     /translation="MEAQPCVYILASKRNGTLYTGVTRYPAQRIWQHKQGLVRGFTKK
                     YSVHRLVYIELHDTMPAAIQREKQIKEWKRAWKLQLIESVNPNSHDLYEDFCR"
BASE COUNT         1143 a         1989 c         1972 g         1148 t
ORIGIN      
        1 gatcgccggc cttggcgcct tcgatctcct gcctcaagtc gagcacggca atacccatgg
       61 catcgaagca ggagaggaaa aacaccgggg tcttggcggg atcgaccggt ccgtaactca
      121 aggacacaaa tccgtcgctc gccttctcgg tccagatcag gctttccgcg gcgcgcgcca
      181 cgcctccaag cgccgccaac aaaagccaag cgacaaggac gagatgcacc ctcacgcggt
      241 gtgtcatttg aacgctttct ttggggggaa gcccgaaccg gaccgtgacc ggcctagagg
      301 ctaccggcgt gaagcctgct ctccgccatg accacctgca ccgtggggaa gtagccggtg
      361 aagttcggat cgaattcgtg gtagttgcag gtgagctcct cgccttgggc aacatcgaac
      421 agcgcgtagc ccttatcgaa gatgcagaag tcggtgttgg ggctaaggct atgattcatg
      481 aagcgcccgt tgtcgctatc gagaatcacc acccccggga tctcgagatg cgggtaggtg
      541 tagcgagcaa cgaagtcctg caggaagggc ggataggccc cgatctcgct tggatgtatg
      601 aagatatcga atttcgggtt gaggctccaa atgagccgcc cccgttcgat atattcgccg
      661 gcgaacacgc cgatgccgtc gatggcgctc ggcgcaaggt aggtgctgac cgtcagcatc
      721 gcgatgcccc ttgatttttt ggctgaagcg caggcgaact catgcggtac tttccgcggc
      781 ctccgtgccg acagcgacca ggctgcgttt gcgccggaag cgccgccgct tcagagccct
      841 gatgcgccgt tccagatacg gcgagaaatg tccggcgtaa cgctcccaca gctcgggatt
      901 gcgccaatcc tccccggtca cgcggccgcg gcaggcggcc gatccgcagg cacactcgaa
      961 ttcatcgtaa ggactgccgt cgcacatcgc atagtcgatg gtgacttcct cgccgggatg
     1021 aatccggttc agggcgacaa tggcgatctg cccattgagg ccggcattcg gttcgcaact
     1081 gtgattgatg aagtccggcg gctcagcctg cgagtgcgag gcgagataaa gtccctcctc
     1141 gacctgcacc gtatgccgcc tgatcggttc cggaagctcg tcgagcgttt cggccgagat
     1201 gatgcggccg ctccagacgc caataagctc gccgggttcg atgatgttcc gagcgtagac
     1261 ggctttgccg cctgttacgt cacgattttg gacttcacat ttgtcggtaa ggtaagccgt
     1321 ggatccctgg ctcacgagac ctccgcgtat gcgcgccgag cggggcgctc cgcagtatat
     1381 tgtgagcggt gcccggccgc tcccaggctc gacaccgcgc gcgagactcc ccaacgcgcg
     1441 gaggagcata agagaagctg ttgtgcccat ctacaaaaaa acgcgcctcg atcaattatt
     1501 ggtggatagc ggtttggcgc cgacgcgctc ccgcgctgcg gacctcatcc ggcgcggctg
     1561 cgtcagcgtg gcggggaagc ctcagctaaa gcccggcgcg ctggtcgcgc ccggcgcgtc
     1621 atttgccgta agcccggaga cctgcgccta cgtgtcccgc ggcggactca agctcgcggc
     1681 cgcgctcgac gccttcggct tcgatcctag ggggagggtg gcgctcgatc tcggcgcctc
     1741 gaccggcggc ttcaccgaag tcctgatcgg gcgcggcgcc gccaaggtct acgcggtgga
     1801 cgtgggaaag gggcagctcc atgccaagct cagggccaat cccagagtca tcgcgctcga
     1861 aggcaccgac gcccgggccc tcgataagtc cgtgatcggg gaggaggtga cggcaatcgc
     1921 cgccgatgtg agcttcatcg gtcttgcctt ggcgcttccg gcagccttgc gcttggccgc
     1981 gctgggggct tggctcgtgg ctctcatcaa gccgcaattc gaggccgggc gcgaagcggt
     2041 gggaaaaggc gggatcgtgc ggagcgaggc cgaccggcgc aaggccgtgg ccaagatacg
     2101 gaaattcatc gaagagcagc cgggctggac cgtcatcggc gaaatcccat cgccgatccg
     2161 gggcgggagc ggcaacgagg agttcctgat cggcgcccgc catggagcct gagggtgtta
     2221 ttcagccgat tttctcgcgt cctgttcgaa ggtgtcgaag atgaccgccg gtttggcgtc
     2281 atcgaacatg cgctgcgtca ggaggatcgc cgtcagatgc tgcgcgggat cgctttgcca
     2341 tttgctgccg aatccgccta cccagccgat ggcgcccgcg ggcgcgccgc tcggcgcctt
     2401 ttccacggcg accgccatgc cgtagcccca accctgaccc tcctgaagga tcatcacgcc
     2461 gtccttgcgc tgcgccggcg taaggtggtt ggtggtcatt gctcgcgccg cattggccga
     2521 tagcagctga tgctcgccgt actttccgcc gccaagcagc atgcgagcga atgccagata
     2581 gtcgtccgcg gtcgacacca ggccggcatc gccctgctcg aagggcggcg gacggctcca
     2641 gccgccggtc gccggatcgt cggagacctc cagcgttccg tccttcggac gatatgccgt
     2701 gaccaggcgg ctgattttgt cccgcgggac atagaacccg gtgtctttca tcccgagcgg
     2761 actgaaaact cgctcggcga gaaaatcgcc gaaggatatg cctgaggcgc gcgctatcag
     2821 cacccccaga atgttcgagc cggtcgtata cagccagcgc tcgccaggct gcgcaaagag
     2881 cggcaaggtc gccaggcgct gaatccactc gtctccgttg aatggcatgt tgggatcagg
     2941 cggtccgaaa ccgacgatgc ccaattccga gattttcttc tggatgggat aaattccggg
     3001 cagcttggta atgaggcccc aaccgaggcg gaacgtcagc aggtcttcca ccgtgatcgc
     3061 acgccgtgcg gggaccgtat cgtcaaccgg cccctcgagc ctccgcagca cccgacgatc
     3121 cgcaagttcc ggcagcaaac gatcgaccgg ttcctcaagc ttgagctttc cgtccgcaac
     3181 aagcatcatc gtggctgagg ccgtgatcgg cttgctcatc gaggcgatgc gaaaaatcga
     3241 gtcgcgttgc atcggcgggc catccaccgc catgcgaccc aacgcggcaa cgtggatgtc
     3301 gtcgccgcgc gcgaccaatc cgactagccc tggtgcgtcc ggccttcagg gccttctcca
     3361 gattggcgtc cagctcgctc accccagtca gcgaatatgc gtgcctgggc aaggcggtcg
     3421 cgaccgccga ggccgccagc aacctcagta tcgctcttcg atcgatcacc tgaagcatcg
     3481 tatgactctt gtggcgtcta cctctccaac cacgcaaaaa gccgcccgat tgagtcccct
     3541 ataccgctag aacggaaatc aaccccgatg gttgcttaat gcttttctgt tctcgcctca
     3601 tgtcgagatg gttgcccatc tcgaacgcta ggcgcggagc aaacgctcga cgaagctcgg
     3661 cacgatctcg gtcgcaggcc cgtaatgggc ctcggtgaag aggctcgcgc cgtcggaagg
     3721 ctcgaggttg agctcgaccg tatgcgcacc ggccgcgcgc gcctccgtaa cgaagcccgc
     3781 ggcggggtag acgctaccgc tcgtgccgat ggagatgaac aggtcgcagg cggtcagagc
     3841 ggcgtagatg cggtccatct cgcgcggcat ctcgccgaac caaacgacat cgggccgcat
     3901 cgtgccggcc ttgccgcaac tgccgcagac ggttccggtc gagagatcgt gggtccaacc
     3961 gacgctcgtt tcgcaggcgg cgcaaaggac ccgggccagc tcgccatgca tgtggatgag
     4021 attctcggtg ccggccgctt catgcagcgg gtcgatattc tgggtcacgg tgagaacatt
     4081 gccccgatac tcgcgctcga gccgcgcgag tgcgagatgc gccgcattcg gctgtaccga
     4141 gccgaggcgc ctccggcgca gattgtagaa atcatgcacg cgcgctggat tgcgtgcgaa
     4201 gccttgcggc gtcgccaccg cttcaagatc gaactcctcc cacaatccgc ccttgtcgcg
     4261 aaacgtgccg agcccgcttt ccgccgaaag cccggctccg gtcaggacga cgatgtgctc
     4321 gaatctcatc ggtgtcatgc ccgcgcaggc gggcatccag ttcccgctga tctttgtgat
     4381 cgatcactct ggtagctact ggatcgcccg gtcctgcctg cgcgaagccg aagcgcctgc
     4441 tgcatcaaaa cgccatcaac ggacggcttc gcttcggcga aggcaggcaa gcgggcgatg
     4501 acaacgagaa cgggcaggga gttacccttt gccggtctgg ccgcggtgcc tgagatagtg
     4561 gtcagcaagc acgatcgcga ccatcgcctc gccgatgggg acggccctga tgccgacgca
     4621 aggatcgtgg cgtcctttgg tcgaaatctc ggtgtcgtgg ccctttgcgt ccacggtgcg
     4681 gcgcggcgtc aggatcgagg aggtcggctt taccgcgaag cgcgcggcga tcggctgacc
     4741 ggtcgagatg ccgcccaaaa tgccgccggc gttgttcgac aggaattcgg gcgtgccgtc
     4801 gttcctcatg cgcatctcgt cggcgttttc ttcgcccgat agcgccgccg acacgaaccc
     4861 tgcgccgatc tcgacgccct tgaccgcgtt gatgctcatc agccccgcgg cgatgtcctg
     4921 atcgagcttg ccgtagaccg gcgcgccaag gcccggcggc acgcccgcgg ccacgatctc
     4981 gatgatcgcg ccggccgagg aacccttctt ccggacttga tcgagaaagc cggcccagac
     5041 ctcggccgcc ttggcgtcgg gggaaaagaa cggattgttt cccacctcgt tccaatcgaa
     5101 gttgcgccgg tcgatcgcat gagggccgat ctggacgagc gcgccgcgca cgaccatgcc
     5161 gggcaccact ttgcgcgcga tcgcgccggc agcgacccgg gcagcggtct cgcgcgcact
     5221 cgcgcgcccc gagccgcgcc aatcgcgcaa tccgtatttc atcaggtagg tgtagtcggc
     5281 gtggcccggg cggaacttgt ccttgatatc gccgtagtct ttgggccggg catcgacatt
     5341 ttcgatctcg agcgcgatcg gcgtgcccgt cgtgacctgc gcgccggact cgtcggtgaa
     5401 cacgcccgac aagatgcgca cgcggtcggg ctcctgccgc tgggtggtat agcgcgactg
     5461 gcccggtcgc cgcttgtcga ggtaagcctg gatctcctcc tcggtgatgg agattccggg
     5521 cggacagcca tcgaccacgc agccgatcgc cggcccgtgg ctttcgcccc aagtggtgat
     5581 gcggaacaga tggccgaaac tgttgaaaga cacggagttc ttttaagagc agggtggacc
     5641 cgcggcaagc ggcgcgaacc tagcgcgaac ttcgcaacaa accgtttcgt cagtcggcgt
     5701 aaggcggaat ttggggctgc tagctcagag ctgttgctcc aaaccccggc tttcaccgcg
     5761 gtgacgggta atgtcgatgg gccctcgtca ttccggcgta agccggaatc cagtacgtca
     5821 ttccggcgta agccggaatc cagtacgact taggcatgga agctcagcct tgcgtttaca
     5881 ttctcgcaag caagcgaaac ggcacgctgt ataccggcgt cacccgctac ccggcccagc
     5941 ggatttggca gcacaaacag ggtctcgtcc ggggtttcac gaagaaatat agcgtgcacc
     6001 ggctcgtcta tatcgagttg catgacacga tgccggccgc aattcagcgc gagaagcaaa
     6061 tcaaggaatg gaagagggcg tggaagctcc aactgatcga gagtgtaaac ccgaactcgc
     6121 atgacctgta cgaagatttc tgccgctaga gtcatcattg tcgtcattct ggcgtaagcc
     6181 taaatccagg accgctgccg ctggctcaga gcttgttgct cctggacccc ggctttcgcc
     6241 ggggtgacgg gt
//
LOCUS       DASUQP010000249         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1078144, whole genome shotgun sequence.
ACCESSION   DASUQP010000249 DASUQP010000000
VERSION     DASUQP010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078144"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1137)
                     /gene="plsX"
                     /locus_tag="VFY74_11440"
     CDS             complement(94..1137)
                     /gene="plsX"
                     /locus_tag="VFY74_11440"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5932341.1"
                     /translation="MGAPVTIALDAMGGDHGPSVVIPAAAMTAIRHPEARFILVGDAA
                     RIEPELSAHPALAAKSELLHTDIAVAMDAKPSQALRKGRWKSSMWLALEAVRDGRADA
                     AVSAGNTGALMAMAKFCLKTIPGIDRPAIAAIWPTIESECIVLDVGANVGADSRQLVE
                     FALMGAAMARALFGVERPTVGLLNIGVEEVKGVDEIKKAAAVLKEAPLPIRYHGFVEG
                     DDVGRGAVDVVVTDGFTGNIALKTAEGTAKQIGEYLRAAMGRSLLARIGAVLAQGAFR
                     ALKEKMDPRQLNGGIFLGLNGIAVKSHGGTDATGFASAVDLAYDMASNGVVKRLASDI
                     AGFHAKLELEGAA"
     gene            complement(1264..1809)
                     /locus_tag="VFY74_11445"
     CDS             complement(1264..1809)
                     /locus_tag="VFY74_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX5932342.1"
                     /translation="MSEDIGRQVLSRPFKVDEIRDGASGEIAVTESELEAIAKMLDLA
                     ALHQLNFAYSFEHRGGGRLLLTGTLRAMLTQTCVISLEPIEARLEVPVEMEFWPAALM
                     PHSERNAADPGTSGLLDWPEAIVEGRIDLGPVIYESLATALDPYPKREGARFRWAEDE
                     GEEAETAKTGPFAALAALKQR"
     gene            complement(1821..2372)
                     /locus_tag="VFY74_11450"
     CDS             complement(1821..2372)
                     /locus_tag="VFY74_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX5932343.1"
                     /translation="MNPFTSFTTRRRSRSDAAKLYGAIVAQARLPVFYQRFEVPDTLE
                     GRFLVLLLHLFVVHHRLKAEGQSALDLAQDLADRFSADMETVLREIGVGDLGIPKKVR
                     GVAAAGASLLEALERALASGDEAVVAAVTGALPADQRLSEAASLRLAHYVRRSVRALE
                     AESVTALAAGAIRFPGTNDRPEA"
     gene            2461..2961
                     /gene="bamE"
                     /locus_tag="VFY74_11455"
     CDS             2461..2961
                     /gene="bamE"
                     /locus_tag="VFY74_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX5932344.1"
                     /translation="MPRLKLLSVTASRPMLAAVLLGVGLLSGCGAQIDRHGHVFIDVD
                     INSVQPGMTKEQVTAVLGSPDTTSTIAGEAYYYISTTQKTVAFLKAREIDRQVVAVYF
                     DGGANVQQVAHYGLKDGIVVNYLKDETPARGKDLSFLEQIFGNISNRGMFKDMRPPAS
                     TGAPPI"
     gene            complement(2981..5104)
                     /locus_tag="VFY74_11460"
     CDS             complement(2981..5104)
                     /locus_tag="VFY74_11460"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5932345.1"
                     /translation="MTWVLFLIIACGALSIAYGVWTTRAVLASDPGTARMQEIAAAIQ
                     EGAQAYLARQYTTIAIVGVVIFAILAYLLGIPQAIGFAVGAVLSGVAGYIGMNVSVRA
                     NVRTAQAATKSLADGLSLAFRAGAITGLLVAGLALLGVSIYFFILTRIVGYDPASREV
                     IDALVALGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNV
                     GDNVGDCAGMAADLFETYVVTLVATMVLASIYFAGGEDRVMQMMLYPLAIGATCVITS
                     IIGTYFVRLGKSQSIMGALYKGVIATGVLSLILLWPLTAAFVGMDTAMKVGEVSFTGR
                     SLFYCGALGLVVTALIIVITEYYTSTRYRPVHSIAKASVTGHGTNVIQGLAVSMESTA
                     LPALVIMAGIVGAYLLAGLFGIAIAVTTMLALAGMIVALDAFGPVTDNAGGIAEMAGL
                     PKDIRKTTDMLDAVGNTTKAVTKGYAIGSAGLGALVLFAAYTEDLKFFAANADKFPYF
                     AGVDPTFTLSNPYVVVGLFLGGLLPYLFGGIAMMAVGRAGSAIVEEVRRQFREKPGIM
                     KGTERPDYGRAVDLLTRAAIKEMIVPSLLPVLSPVAFYFVIEWIAGKSAAFSALGAML
                     LGVIVTGLFVAISMTAGGGAWDNAKKYIEDGNYGGKGSEAHKAAVTGDTVGDPYKDTA
                     GPAVNPMIKITNIVALLLLAVLAHQ"
BASE COUNT          969 a         1632 c         1685 g          900 t
ORIGIN      
        1 caaggtcgtc attggtcaaa acccgctcgg gcaggtatcc cccgcatcct ctcaccaccg
       61 tccttacgat atccaacgtc atcactccaa aatttatgcg gccccttcga gctcgagctt
      121 cgcgtggaac ccggcaatat cgcttgccag gcgtttgacg acgccgttcg acgccatgtc
      181 gtaggcgaga tcgacggcgc tggcgaagcc ggttgcatcg gttccgccgt ggcttttgac
      241 ggcgatgccg ttcagcccta ggaatatgcc gccattgagc tggcgcgggt ccatcttctc
      301 cttgagggcg cggaacgccc cttgcgccag gacggcgccg atgcgcgcaa ggagggaccg
      361 ccccatggcg gcgcgcagat attcgccgat ctgcttagcc gttccttccg ccgtcttcaa
      421 ggcgatgttg ccggtaaacc cgtcggtgac cacgacgtcg acggcgcccc tgcccacgtc
      481 gtcgccttcg acgaagccgt ggtagcgaat ggggagcggc gcctccttga gaacggcggc
      541 cgccttcttg atttcgtcga cgcccttgac ctcctcgaca ccgatattga ggagcccgac
      601 cgtgggtctt tccaccccga agagcgcccg cgccatcgcc gcgcccatca gggcgaactc
      661 gacgagctgg cgcgagtcgg caccgacatt ggcgccgaca tcgagcacga tgcactcgct
      721 ctcgatggtc ggccaaatgg cggcaatggc ggggcggtcg atgcccggga tcgtcttcag
      781 gcagaacttc gccatcgcca tcagcgcgcc ggtattgccg gccgacacgg cggcgtcggc
      841 gcggccgtcg cgcaccgcct cgagcgcgag ccacatgctc gatttccagc ggcccttgcg
      901 cagcgcctgg ctcggctttg cgtccatggc gacggcaatg tcggtgtgca ggagttccga
      961 cttcgcggcg agcgcagggt gggcgctgag ctcgggttcg atccgggcgg cgtcgccgac
     1021 caggatgaaa cgcgcctcgg gatgacggat cgcggtcatt gccgcggcgg gaatgaccac
     1081 gcttgggccg tggtccccgc ccatggcgtc gagcgcgatg gtgactggag cacccatgat
     1141 ttgctgccgt tcaggaagaa cgatttatgc ggcgggacgg ccgccgcaac ccccggaaaa
     1201 ctggacccga atagacgcaa aaaggcggcc agtgacaacc ggattttgct tgggtgggtg
     1261 ggcctagcgc tgcttcaggg cggcaagcgc cgcaaatggg ccagtcttgg cggtttcggc
     1321 ctcctcgccc tcgtcttccg cccaccggaa gcgggctcct tcgcgctttg gatagggatc
     1381 gagcgccgtg gcgaggctct cgtagatgac cggcccgagg tcgattctac cctcgacaat
     1441 ggcctctggc caatcgagaa gccctgaggt tccagggtcg gcggcatttc gctcggaatg
     1501 gggcatcagc gcggccggcc agaattccat ctcaaccggc acctccaggc gcgcttcgat
     1561 gggttcgaga gagatgacgc aggtttgtgt cagcatggcg cgaagcgtgc cggtcagcag
     1621 cagacgccca ccgccgcggt gttcgaagct ataggcgaaa ttcagctgat ggagtgccgc
     1681 gagatcgagc atcttggcga tggcctcaag ctcggactcg gtcaccgcga tctcgccgct
     1741 tgcgccgtcc ctgatctcgt cgaccttgaa cggtcgcgac agcacctggc gtccaatgtc
     1801 ctcactcatg tcgatcctat tcacgcttcc ggcctgtcat ttgttcccgg aaaccttatc
     1861 gcgcccgcgg caagcgcggt tacgctctct gcctcgaggg cgcgtaccga tctccttaca
     1921 tagtgcgcaa gccgcaagct tgcggcctcg ctgaggcgtt gatcggcggg aagcgcaccg
     1981 gtgacagccg cgacgacggc ttcgtccccc gacgccaagg cgcgctccaa tgcttccagc
     2041 aaggatgcgc ccgcggcggc aactcccctc accttctttg gaatgccgag atcgccgacc
     2101 ccgatctcgc ggagcacggt ctccatgtcg gcgctgaagc ggtcggccag gtcctgggcc
     2161 agatcgagag cggattggcc ttccgccttg aggcggtggt ggacaacgaa aagatgcagc
     2221 aacaacacca ggaagcggcc ttcgagcgta tcgggcacct caaaccgctg atagaaaacg
     2281 ggaagccgcg cctgcgccac aatcgcgcca tagagtttcg ccgcgtcgga tcggctgcgg
     2341 cgccttgtcg taaaggatgt gaaggggttc aaagcacggg ccgtttggtc tagcctttcg
     2401 cgacgggggc gtcacgatcg gcctttggtt ttattcggga tcatcgaggg aacatccgct
     2461 ttgcctcggc tcaagctcct ctcggtcacg gcgtcgcgtc cgatgctcgc cgcagtgctg
     2521 ctcggcgtcg gccttctttc gggttgcggg gcccagatcg accggcacgg acatgtgttc
     2581 atcgatgtcg acatcaactc cgtgcaaccc gggatgacca aggagcaggt caccgccgtg
     2641 ctcggttctc ccgataccac gagcacgatc gcaggcgagg cctattatta catttccacc
     2701 acgcagaaga cggtcgcctt tctcaaggcc cgcgagatcg atcgccaggt cgttgcggtc
     2761 tatttcgatg gcggcgccaa cgtccagcag gtcgcccatt acgggctgaa ggacgggatt
     2821 gtcgtcaatt acttgaagga cgagacgccg gcccgcggca aggatctcag ctttctcgag
     2881 cagatcttcg gcaatatctc caatcgcggg atgttcaagg atatgagacc gccggcatcg
     2941 actggcgcgc cgcccatcta ggccgacggg atgcccgtct ctattggtgg gccagcacgg
     3001 ccagcaacag cagcgcgacg atattggtga tcttgatcat cgggttgacc gcgggaccgg
     3061 ccgtgtcctt gtaaggatcg ccgaccgtat cgccggtcac cgccgcctta tgcgcctcgg
     3121 agcccttgcc cccgtagttg ccgtcctcga tgtatttctt ggcgttgtcc caggcgccgc
     3181 cacccgccgt catggaaatg gcgacgaaca ggccggtgac gatcacgccg agcagcatgg
     3241 cgccgagcgc ggagaaggcg gcgctctttc cggcaatcca ttcgatgacg aaatagaagg
     3301 cgaccggcga cagcaccggc agcagcgacg gcacgatcat ctccttgatc gcagcacggg
     3361 tcagaagatc gacggcgcgt ccatagtccg ggcgctcggt gcccttcatg ataccgggct
     3421 tctcacggaa ctggcggcgt acctcctcga cgatcgccga cccggcgcgg cccaccgcca
     3481 tcatggcgat gccgccgaag agataaggca gcaatccgcc gaggaagagg ccgaccacga
     3541 cgtagggatt tgacagggtg aaggtgggat cgaccccggc gaagtaaggg aatttgtcgg
     3601 cgttggcggc gaagaacttg agatcctcgg tataggccgc aaacaatacc agagcgccga
     3661 gccccgccga gccgatggcg taacccttgg tcaccgcctt ggtggtgttg ccgacggcat
     3721 cgagcatatc ggtggtcttg cgaatatcct tcggcaaccc tgccatctcc gcaatgccgc
     3781 cggcattgtc ggtgacaggg ccgaaggcat cgagcgccac gatcatgccc gcgagcgcca
     3841 gcatggtagt cacggcaatc gcgatgccga agagtccggc aagaagatag gcgccgacga
     3901 tgccagccat gatcacgagt gccggcagcg cagtcgattc catcgacacg gcgaggccct
     3961 ggatcacgtt cgtcccatgg ccggtgactg aggctttggc aatcgaatgg acggggcgat
     4021 accgcgtgct ggtgtaatac tcggtgatga cgatgatgag cgcggtgacg acgagaccga
     4081 gcgcgccgca gtagaacagg gagcggccgg tgaagctcac ctcccccacc ttcatggcgg
     4141 tgtccatgcc gacgaacgcg gccgtcaagg gccatagcaa gatcagcgag agcacgccgg
     4201 tggcgatcac ccccttgtac agggcgccca tgatcgactg gctcttgccc agccgcacga
     4261 aataggtgcc gatgatcgag gtgatgacgc aggtagcgcc gatggcgagc gggtaaagca
     4321 tcatctgcat gacgcgatcc tcgccgccgg caaagtagat ggaggcgagc accatggtcg
     4381 ccacaagcgt gaccacatag gtctcgaaca ggtcggccgc catgccggca cagtcgccga
     4441 cgttgtcgcc gacgttatcg gcgatcgtcg ccgggttgcg gggatcgtcc tcgggaatgc
     4501 ccgcttcgac cttgcccacg agatcgccgc cgacatcggc gcccttggtg aagatgccgc
     4561 cgccgaggcg ggcaaagatg gagatgagcg atgccccgaa gccgagcgcg acaagggcgt
     4621 cgattacttc gcgcgaggcg ggatcgtagc cgacgatgcg cgtgaggatg aagaaataga
     4681 tcgagacgcc gagaagggcg agaccggcga cgagaagtcc ggtaatggcg ccggcgcgga
     4741 aggcgaggct caagccgtcg gcaagactct tggtcgcggc ctgagcggtg cggacattgg
     4801 cgcggacgga gacgttcatg ccgatatagc cggcgacgcc cgacagcacc gcgccgacgg
     4861 cgaagccgat cgcctgcggg atgccgagga gataggcgag gatcgcgaag atgacgacgc
     4921 cgacgatggc gatcgtggtg tattggcgcg cgagataggc ttgcgcccct tcctggatgg
     4981 ccgccgcgat ctcctgcatg cgcgccgtgc cggggtccga ggcgagaacg gctctggtcg
     5041 tccagacgcc ataggcgatc gaaagcgccc cgcaagcaat aatcagaaag agaacccagg
     5101 tcataaggct gcctctatgg tttcgtggta ttgacgggga gatacggcga aaaaggcggg
     5161 gcgcccgatt tgccccaggc ccgaaa
//
LOCUS       DASUQP010000250         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1350076, whole genome shotgun sequence.
ACCESSION   DASUQP010000250 DASUQP010000000
VERSION     DASUQP010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350076"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFY74_11465"
     CDS             complement(<1..693)
                     /locus_tag="VFY74_11465"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX5932346.1"
                     /translation="MTTWLALFILAFAAILLVADESGMIAGLDAATFGYVALFLALLV
                     FIGGGMLGSYRGRAGTMVRDAVTWLALGLGLIAIYAYKDELMPIAARVVGELLPGSAM
                     TVEQSSGGVTEVRIRKRLDGHFTANVTVNGKEVSMIVDTGASSIVLRPQDARKAGIDV
                     KRLTYRVPVLTANGRTLAARVRLEEVAIGPLDRKKVDALVAQPGALSQSLLGMSFLSR
                     LRSYEFSGDFLTL"
     gene            complement(690..875)
                     /locus_tag="VFY74_11470"
     CDS             complement(690..875)
                     /locus_tag="VFY74_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX5932347.1"
                     /translation="METPCVNICLLDPATGTCLGCGRTLAEIASWAAMSDAERRAVMA
                     ALPARMERRQKGERLTA"
     gene            complement(1306..1833)
                     /locus_tag="VFY74_11475"
     CDS             complement(1306..1833)
                     /locus_tag="VFY74_11475"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5932348.1"
                     /translation="MDRLRLLAVTMLAAGFVCVAGKPASAAVMIVIDKPSQTMTVAVN
                     GSVQYRWYVSTGATGYSTPSGSYTPFRLEVMHYSREWDNAGMPHSIFFTSRGHAIHGS
                     DHPGRGTPRSHGCVRLSLTNAATLFRLVAAEGMANTRVVVRGADPPGVYAATQIPTRK
                     RAKPLHKGFLFPFMF"
     gene            complement(3084..4553)
                     /locus_tag="VFY74_11480"
     CDS             complement(3084..4553)
                     /locus_tag="VFY74_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5932349.1"
                     /translation="MQAAREKLASKSGAKPEFEYELLSMFVRNELSAAVATPLLAIIV
                     AMGAMFWAPSRELLLWLATVFVAKGILVALCRQFAKAPRDDAETHTWRAKITAAEFLY
                     GVCWAGVAFISPDTQSEAAHTFIFATLIVVISMRVLFASTVKSIVYAGTLPMTAAIVI
                     RFVLLDNPFYWAMAAMAVGVYIYFIWLMKGLNTTVVAMLEFRAEKDALIAELEQAKSI
                     SDEARFRAEEANLAKSRFLATMSHELRTPLNAILGFSEIMRAEILGPHANPTYKEYAG
                     DIHQSGQHLLNLINEILDISRIEAGRYELREAPLDLARVAEDCQRLLRLRAENKGLNL
                     VEDFEPDLPQLWADEQAVRQICLNLLSNAIKFTPRGGTVMLKIGRSAGGGQYLSVKDT
                     GPGIPESEIPRVLKSFGQGSLAQENAEGGTGLGLPITKGLTELHDGTFELKSRLRHGT
                     EVIVAFPKKRVMEALPRVPEPHEIEAKPVRATWHARERR"
     gene            4801..>5257
                     /locus_tag="VFY74_11485"
     CDS             4801..>5257
                     /locus_tag="VFY74_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5932350.1"
                     /translation="MQLTIIGCGDAFGAGGSLQTSFLVRGSGSTFLIDCGTSTLIGMR
                     RLGLKPNDVDTVFVTHLHGDHFGGLAWLLVDAIYASERKRPLLVTGPKSIARRFATAA
                     EALYPGITKIKRKFDLTFLEYEERKPLVAGGVTVIPFEVHHPSGAPPYAL"
BASE COUNT         1018 a         1616 c         1645 g          978 t
ORIGIN      
        1 cagcgtgagg aaatcgccgg agaattcgta ggagcgcagc cggctcaaga aactcatgcc
       61 gagcaggctc tggctcaggg cgcccggctg cgcgacaagg gcgtcgacct tcttgcggtc
      121 gagcgggccg atggcgacct cttcgagccg cacccgcgcg gcgagcgtgc ggccgttggc
      181 ggtgagcacc ggaacacgat aggtcaggcg tttcacgtcg ataccggcct tcctggcatc
      241 ctggggccgg agcacgatgc tcgatgcgcc ggtatcgacg atcatggaga cttcttttcc
      301 gttcaccgta acgttcgcgg tgaaatgacc gtcgaggcgc ttcctgatgc ggacttcggt
      361 gacgccgcca gagctctgct ccacggtcat ggcgctgccg ggaagcaatt cgccgacaac
      421 gcgcgccgcg atcggcatca gctcgtcctt gtaggcatag atggcgataa gccctaaccc
      481 aagcgcaagc caggtcaccg catcgcgcac catcgtgccc gcacgtccgc gataactccc
      541 cagcatgccg ccgccgatga agacgaggag ggcgaggaaa agagccacat agccgaacgt
      601 ggccgcgtcg aggccggcga tcatgccgga ctcgtctgcg accagcagga tggcggcgaa
      661 ggccaggatg aagagggcga gccaggtcgt cacgcggtca gcctttcgcc tttttgccgt
      721 cgctccatac gcgccggaag cgcggccatc acagcgcgcc gctccgcatc gctcattgcc
      781 gcccagcttg cgatctcggc aagcgtgcgc ccgcagccaa ggcaggtgcc ggtcgccggg
      841 tcgagcaggc agatattgac gcaaggcgtt tccaaagcgt gttccgcaag gccaatggtc
      901 cctgcgcgaa tgtagggatt gccgggcttg ccagcaatcg ccgccgcgac gacgcgtcga
      961 tgaccctctt gcgggtcgtc agttcgctga tcgtcggagg cggcgaaacc atagccattc
     1021 gctattgggc agcggcgcgc cattcgccca tcttacggat gttcgaacgg aacatttggc
     1081 cagccgggcg aacgcaagtt caaccggcca agctcaagga gaacccttat gaagaagctc
     1141 attatcgccg ttgcgctcgt ggtcgcctct gccagccccg tttgggccga cgctttcccg
     1201 aacgtctaca tgacccgcta aaccgggtcg aaatcgatgg tcaagcgacc atcaccgcct
     1261 tctctcccca tgggcacaag tgacacgctt gtgcccattc ccttgtcaga acatgaacgg
     1321 gaataagaac cccttgtgca agggcttggc ccgcttccgc gtcgggatct gggtggcagc
     1381 gtagacgccg ggaggatcgg cgcctctgac cacgaccctg gtattcgcca tgccttccgc
     1441 cgcgaccagc cgaaagagcg tggcggcgtt ggtcagcgag agacgcacgc agccatgcga
     1501 ccgcggcgtg ccccggccgg gatggtccga gccatggatg gcgtgccccc gcgaggtgaa
     1561 gaagatcgag tgcggcatgc cggcgttgtc ccattcccgc gagtaatgca tcacctccaa
     1621 gcggaacggc gtgtaggagc ccgacggcgt cgaatatccc gtggctcccg tcgatacgta
     1681 ccagcgatac tggacgctgc cattgaccgc gacggtcatg gtctggctcg gcttgtcgat
     1741 tacgatcatg acggcggcag aggccggttt gccggcaaca caaacgaagc ccgccgcgag
     1801 catggtgacg gcaagaagcc gaagacgatc catctccgtg ttccctcgtg tttttcaaag
     1861 ccgcgcgata aggcccgcgc gacattggcc ccgtaagacc ccagtctagc aggctgggcg
     1921 gcccccgtca tcgcccgccg accggggccg caaggcttcg agcaggtaag tcgtgtattc
     1981 ataaattggc attatttgag tcgtggcgcg gaatgtctgc ttagccccca atagcggacg
     2041 tgcggccgcc cgctcaaaga gtccgccaat tgccaacaaa agacattgag gcgacccaac
     2101 cgaaaagcat tggggccaca ttgaaggtgc acttcacggt gcgtcctgtt cgccggcctc
     2161 cctccgttcg tcgattatga gtggttgctg aaggggcgcg gccattaagc ctagcaagtg
     2221 tctctgctac tttaacaaat aactgatatt gtgaacgagg aagtgagacg ccgatcagat
     2281 gtcgtggtta gtagtaagga gagtgccatg acaagagccg tgacaaaggc agctggcttt
     2341 attcagcttc tctttgggga gtcacgcggg caggatgccg gtgatgcatg ctcgaactgg
     2401 tttcccggcg aggggggaaa gatgttgggc accattcccg actgcgtttg ctcgaaacca
     2461 cccacgtgcg aagcaaacta tacttgccct gaaggaacta tcggttcagg tgagtttccc
     2521 gactgcttct gcccgataga ccagaagaaa ttgcccgagc ctcctaattg cgaagctaat
     2581 tgggagtgcg ttacggacgt gctcgtgggc accggcaaat ggccaagctg ttggtgtgta
     2641 gaacgcgaca gtggcaagcg acctggaggt ggtggcaacg gtggtggaag cggaggtatt
     2701 ggcacttcaa tgggagggct tgacacctgt cgccagttct tcgactgccc agcgtgctac
     2761 aaaatgatct tgtccagcag ccaatgtctg tgcgagccga tcatgttaaa ggctgaaaac
     2821 gaggacaagc gctgttgatt gtcgggcacg agtaccccaa gcacatttgg acgaatatct
     2881 tatttcgtgg cggcttgctc cgatgggtga agacgcgcga cgttgaagct gagggatttt
     2941 gccctagaca catttactga agtcgcaagc cgtccgcttt gggtcaaaag cggactctct
     3001 tcacagcacc attctaccac tgcttccccc gatagcggac gcgtcagcgt tctgggcggc
     3061 agcgagattt atgaggacac gtcctagcgc cgttccctcg cgtgccaggt cgcgcggacc
     3121 ggcttcgcct cgatctcgtg cggctccggc acacggggaa gggcctccat gacgcgcttc
     3181 ttcggaaagg cgacgatcac ctcggtgccg tggcggagcc tgctcttcag ctcgaaggtg
     3241 ccgtcatgca gctcggtcag gcccttggtg atgggaagcc cgagcccggt gccgccttcg
     3301 gcgttctctt gcgcaagcga gccttgcccg aagctcttga gcacgcgcgg gatctcgctc
     3361 tccggaatgc cggggccggt atccttcacg ctcagatatt ggccgccgcc cgcgctgcga
     3421 ccgatcttca acatcacagt gccgccgcgc ggcgtgaact tgatggcgtt cgacaagagg
     3481 ttgaggcaaa tctggcggac ggcttgctcg tcggcccaga gctgcggcaa gtccggttcg
     3541 aagtcctcga cgagattcaa gcccttgttc tccgcgcgca gccgcaagag ccgctggcag
     3601 tcctccgcca cccgcgcaag gtcgagcggc gcctcgcgca gctcgtagcg cccggcctcg
     3661 atgcggctga tgtccagaat ctcgttgatg aggttgagca gatgctgccc gctctgatgg
     3721 atgtcgccgg catattcctt gtaggtcgga ttggcgtgtg gaccgagaat ctcggcgcgc
     3781 atgatctcgg agaagccgag gatggcgttg agcggcgtgc ggagctcgtg gctcatggtg
     3841 gcgaggaagc gcgatttggc caggtttgcc tcctcggccc tgaagcgcgc ctcatcggag
     3901 atcgatttcg cctgctcgag ctcggcgatc agcgcgtcct tttcggcgcg gaactccagc
     3961 attgcaacga cggtcgtgtt gagccccttc atcagccaga tgaaatagat gtagacgccg
     4021 accgccatcg ccgccatcgc ccaatagaac gggttgtcga ggagcacaaa gcgaatgacg
     4081 attgcggcgg tcatgggcaa agtgccggcg tagacgatcg acttgaccgt cgaggcaaac
     4141 agcacccgca tcgagatgac gacgatcagc gtggcgaaga tgaaggtatg cgccgcttcg
     4201 ctctgcgtgt cgggactgat gaaggcgacg cccgcccagc agacgccata gaggaactcg
     4261 gcggcggtga tcttggcgcg ccaggtatgc gtctcggcgt cgtcacgcgg cgccttggcg
     4321 aattgccggc acaatgcgac gaggatgccc ttggccacga acactgtggc aagccacagc
     4381 agcaattcgc gcgacggcgc ccagaacatg gcgcccatgg cgacgatgat ggcaaggagc
     4441 ggagtcgcaa ccgcggccga cagctcgttg cgcacgaaca tggagaggag ctcgtactcg
     4501 aactccggct ttgctcccga tttggaggcg agcttttcgc gggccgcttg cacgctgcgg
     4561 aaggctcgcc gcttgctgct cgaagctgcc ggaaccgacg gttgagcctc ggctgacgcg
     4621 cgcgctgaca tgcttaggcg acccgcaaac gccctgacgg acaaaaaaca agacctccca
     4681 aagacatagc ttcgggatgc cccacccggc ctcgcaaagg ccggcctcga actatcagcc
     4741 gaaatcccta acagtgcgtt tactctcgct ccgtgctggg acggcgataa gatcgggacg
     4801 atgcaactga ccatcatcgg ctgcggcgat gccttcggcg ccggaggcag cctccagacc
     4861 tcgttcctgg tgcgggggtc cggctccacc tttctgatcg attgcgggac ctcgacgctg
     4921 atcggcatgc gccggctcgg gcttaagccg aacgatgtcg acaccgtgtt cgtaacccat
     4981 ctccacggcg atcatttcgg cgggctcgct tggcttctcg tcgacgccat ctacgcgagc
     5041 gagcgaaagc ggccgcttct tgtcactggc cccaagagca tcgctcggcg tttcgcaacc
     5101 gccgctgagg cgctctatcc cggcatcacc aagatcaagc ggaaattcga cctcacattc
     5161 ctggaatacg aggagcggaa gcccctcgtg gctggcggcg tcaccgtcat cccgttcgag
     5221 gtgcatcacc catcgggcgc gccgccttac gcgcttc
//
LOCUS       DASUQP010000251        10005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_124151,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000251 DASUQP010000000
VERSION     DASUQP010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124151"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFY74_11490"
     CDS             <1..319
                     /locus_tag="VFY74_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX5932351.1"
                     /translation="NVYDLLKAYSQQRQRMAVRSLRMPPRAVWSLKDARDELERLLGM
                     TLDWAPLDRLLAEFLVEPELRRTALASSFTASLEMTREGTLELRQSKSFAPLLVRRRE
                     RAA"
     gene            355..1137
                     /gene="scpB"
                     /locus_tag="VFY74_11495"
     CDS             355..1137
                     /gene="scpB"
                     /locus_tag="VFY74_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5932352.1"
                     /translation="MTAKQLSRVEAMPEDDRERERGRPRLPLPFPSNVIKLQSTDRRE
                     KLRIVEALLFAAAEPLDEQALAQHFMEGEDVKALLEELRSVYAGRGVNLVRVAGKWAF
                     RTAEDLSFLLEKQAVEQRRLSRAALETLAIIAYHQPVTRAEIEEIRGVSTSKGTIDVL
                     LETDWIKMRGRRRAPGRPVTYGTTEAFLTHFGFDSIQDLPGINELKGAGLLDSNLPPG
                     FSMPNPDDAPDLREDEEPLEDEPFADEPLGDDDEGEGSDGPA"
     gene            1192..2697
                     /locus_tag="VFY74_11500"
     CDS             1192..2697
                     /locus_tag="VFY74_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5932353.1"
                     /translation="MPRFAARGDVYLRDIADPKKTVKQILWGDWLNIEAETEDGWSKV
                     KWGKDRYWIRTSDCEHERPLEVMFIDVGQGDGCLVVTPKSPPDERVILIDAGEGDNMY
                     RFIRWRFGKLKKKFNFHAAIVTHPDQDHYKGFQKLFEEKQFSFDRVYHNGIAEREGAD
                     VFGPSDATGRYLTDLRPTDVSIRSLYADAEVRGRKLYPKLVHTALTGGRVGAVEMLST
                     RHGREQDGRSWMPGFDPDASPELTIEILGPVVEPDTHDTPRLRWFGDTVGSLGKDPGK
                     TKNGHSVLLRLCYRNFTLLFGGDLNRPAEDFLLRHYGGIGDGPPLADAVEKARKRLRS
                     DVIKSCHHGSADVTDEFLRAVSPFAFGVSSGDEESHVHPRPDLLGRLGRHGRGEAPLI
                     LCTEILRSTRETEDKKLLRRLQALDALIEIPNTPESDKKPLRTERAALQSKLAERNVE
                     IYGAITLRTDGKHLAIAFRMERERKGKSPKRWQIYWFSHDEQRGFVSEHGE"
     gene            2780..3934
                     /locus_tag="VFY74_11505"
     CDS             2780..3934
                     /locus_tag="VFY74_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5932354.1"
                     /translation="MLRFLKARAGGANGRNGRAARGAGRGKAAVTFAAQLTLEEVSRR
                     YGETLALDRVSLDIAPSEILCLLGPSGCGKSTLLRVAAGVERPSSGRILLDGQEVAGP
                     DSFVPPEKRGIGLMFQDFALFPHLTILDNVAFGLKSLTRSEAKTEARAALERVGLAHY
                     ASEYPHILSGGEQQRVALARAIAPRPSVLLMDEPFSGLDSRLREKMREETLAILHETR
                     ATAIVVTHDAEEAMRMGDRVALMHQGKVVQTGKALDLYRAPKDILTARTFSDLNELAA
                     RIEDGSALTPLGRFAANGLPDGAEAIVCVRQRGVRLLAPGEGVPARILDARILGDVAL
                     VEVAVQGLDAPLFARVRESDVPPQGAEVGIVIETGAVLVFAGGNGNRPAP"
     gene            4044..4286
                     /locus_tag="VFY74_11510"
     CDS             4044..4286
                     /locus_tag="VFY74_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5932355.1"
                     /translation="MGLSWSHILIVLVLFVLLFGRGKISELMGDVAHGIKSFKKGMAE
                     DEQAPATETKAIEHERAKPATKSASRAKAPHKQKAG"
     gene            4346..4747
                     /gene="tatB"
                     /locus_tag="VFY74_11515"
     CDS             4346..4747
                     /gene="tatB"
                     /locus_tag="VFY74_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX5932356.1"
                     /translation="MFDIGWSELLVIAVVAIVVVGPKELPKLLRTFGYYAGKLRRAAA
                     EFRRQFDDAMREAELEEVKKAIESVRTETASLDEKAPLNKRFMPPTLEERPQLVEPEA
                     VKPPPKPPRKRAAPKTKSPAKRAKSGAEPSP"
     gene            4744..5571
                     /gene="tatC"
                     /locus_tag="VFY74_11520"
     CDS             4744..5571
                     /gene="tatC"
                     /locus_tag="VFY74_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5932357.1"
                     /translation="MTDQDIDATKAPLIEHLIELRKRLVWALLAIFVAFLVCFWFAKP
                     IYNLLLWPYRLAAGLDAPIEMIYTAPQEFFFTQVKLALFGAIFIAFPVLATQIYMFVA
                     PGLYRNERQAFLPFLVATPLLFLLGAALVYFMAMPLAMKFFLSMQQTGDQQVQIHLTA
                     RVSEYLSLIMALILGFGICFQLPVVLTLLARAGMITAAQLKRYRRHAIFAVFVVAAVL
                     TPPDPISQIALAVPTVLLYEASIYAVKLAEKKHAASAAAATAAAATASAAKPAGKPA"
     gene            5781..7310
                     /gene="serS"
                     /locus_tag="VFY74_11525"
     CDS             5781..7310
                     /gene="serS"
                     /locus_tag="VFY74_11525"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5932358.1"
                     /translation="MLDIKWIRDNEEAFVKGLTNRGYDDPRATLKRLLSLDAQRRATI
                     QKLQEAQARRNAASKEIGSAKAKKDEPAAKRLMDEVAALKDTIQAGEAEEKRLDGELH
                     ALLATVPNTPQSDVPVGPDARANKVLREVGKPETFAFTPKQHFELGEALGLMDFETAA
                     KLSGARFVVLKGALARLERALASFMLDLHTSEHGYIEINPPILVRDDAMFGTAQLPKF
                     AEDQFRTGRPETVDDLLSRLKPVIEGIFAEQKPLIEGSDIRRVEALKMRELLAERLGS
                     WTEELSDAQGIPRTDYWEDLAREAARRYLNLWLIPTAEVPLTNLVRDDIVDEASLPLR
                     VTAWTPCFRAEAGAAGKDTRGMIRQHQFSKVELVSITTPEQSAEEHERMTTSAETVLK
                     RLELPFRTVVLSTGDMGFASQKTYDIEVWLPGQAAYREISSCSNCGDFQARRMNARYR
                     PKGEKQPRLVHTLNGSGVAVGRALIAVLENYQESDGSIRIPEALQPYMGGLTEIAAPP
                     K"
     gene            7407..8090
                     /locus_tag="VFY74_11530"
     CDS             7407..8090
                     /locus_tag="VFY74_11530"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5932359.1"
                     /translation="MASAHDQIGLIMQLRRRGIRDNDVLRAIERVPRDLFVDPAFADH
                     AYHDIALPIDCGQTISQPYVVAFMTERLELDARHKVLEIGTGSGYQAAVLAHLCRRVY
                     TVERWRELQKAAERRFARLGITNVTTIIGDGWLGWPPQAPFDRIIVTAAAKEAPPALI
                     EQLKVGGRMILPLGETRDTQQLVQIDKTEAGLAETPLLPVRFVPLVHGRAGVAARQKD
                     TRTIESDDE"
     gene            8197..9300
                     /locus_tag="VFY74_11535"
     CDS             8197..9300
                     /locus_tag="VFY74_11535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5932360.1"
                     /translation="MRRASFLSDSGRGACLALMAACVGLTAAGCSSTASRFDFPSFSS
                     TASDSSGNADVATTSALPVPEQSVYSQGSGADARLQRTALPSPAYSPAPNRRMGATPA
                     PYAQSPAKGHRIKVAKGDTLSSVSRRYGVPVEAIVSANNLPDGRLAVGQELVLPGVTA
                     AKAAAVAKPAPNVRVVKTEPIAAPKGTSLAEEEAAEAAGNKKQNKASEIAASPETTGP
                     GAQRVAGVDQLPAPDPMSGNSFRWPVQGRIIAGFGTRPDGGHNDGIDVAVPQGTSVKA
                     AENGVVAYSGNELKGYGNLVLVRHANNWVSAYANNAELLVKRGDKVRRGQIIAKAGAT
                     GAVSQPQVHFELRKGSRPVDPTKYMSNQAANAE"
     gene            complement(9319..>10005)
                     /locus_tag="VFY74_11540"
     CDS             complement(9319..>10005)
                     /locus_tag="VFY74_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5932361.1"
                     /translation="RVPLSLLKGIDRMRDTLLANTLRFARGLPANNALLWGARGMGKS
                     ALVKAIHNEIAGLKLVEIHREDLGSLPRALSHLRANPDRCFILFCDDLSFEALDTSYK
                     SLKAVLEGGIEGRPANVVFYATSNRRHLMPREMVENERSTAINPGEAVEEKLSLSDRF
                     GLWIGFHNCSQDDYLAMVQGYVHHYQIAVSDAVLRDEALAWARARGSRSGRVAWQYVQ
                     DLAGRLGTRL"
BASE COUNT         1857 a         3229 c         3206 g         1713 t
ORIGIN      
        1 caatgtctac gatctgctca aggcctactc ccagcagcgc cagcgcatgg cggtgcgcag
       61 cttgcggatg ccgccgcggg cggtgtggtc gttgaaggac gcgcgcgatg aactggagcg
      121 gctgctcggc atgacgctcg attgggcgcc gctcgatcgg ctgctcgccg agttcctcgt
      181 cgagccggaa ctccggcgta cggcgctggc gtcgagcttc actgcgagct tggagatgac
      241 gcgcgagggg acgctggagc tgcgccaatc gaaatcgttt gcaccgctgt tggttcgccg
      301 ccgcgagcgc gcagcatgat gtgatgtgtt gacaaccaac aagagggcac ggggatgaca
      361 gcgaagcaac tgagtagggt cgaggcaatg ccggaagacg acagggagcg cgagcgcggc
      421 cgcccgcgcc ttccgctgcc atttccctcc aatgtcatca agctgcagtc gaccgaccgg
      481 cgcgagaagc tgcgcatcgt cgaggcgctt ctgttcgctg ccgcagagcc gctggacgag
      541 caggccttgg cccagcactt catggagggc gaggacgtca aggcgctgct cgaagagctg
      601 cgcagcgtct atgccggccg cggcgtcaat ctggtgcgcg tcgccggcaa atgggcgttc
      661 cgcaccgcgg aggatctgtc cttcctgctc gagaagcagg cggtcgagca gcgccgcctg
      721 tcgcgcgccg cgctggagac gcttgccatc attgcctatc accagccggt gacgcgcgcg
      781 gagatcgagg agatcagagg cgtgagcacg tcgaagggca cgatcgacgt gttgctggag
      841 accgattgga tcaagatgcg cgggcgccgc cgcgcgcccg gtcggcccgt gacctacggt
      901 acgacggaag cctttctcac ccatttcggc ttcgactcca ttcaggacct gcccggcatt
      961 aacgagctga agggcgcggg cctcctcgat tccaacctgc cgcccggctt ctccatgccg
     1021 aaccccgacg atgcgcccga tttgcgcgaa gacgaggagc cgctcgagga cgagccgttc
     1081 gcggacgagc cgctcggcga cgacgacgag ggcgagggga gcgacgggcc cgcttgatcg
     1141 gcgcgctgca gctcaaccgc ctccgtttgc cttagagatt tggaggtagc gatgccacgg
     1201 tttgctgcgc ggggagatgt ctatcttcga gacatcgccg acccgaaaaa gacggtcaaa
     1261 cagattctgt ggggcgactg gctcaatatc gaggcagaaa ctgaggacgg ctggtccaag
     1321 gtcaaatggg ggaaggaccg ctattggatc aggacgagcg attgtgagca cgagcgaccg
     1381 ctagaggtca tgtttatcga tgtcggccaa ggggacggtt gcctggttgt cacgccgaaa
     1441 tcgccgcccg atgagagggt catcctgatt gatgccggcg agggcgacaa catgtatcgc
     1501 ttcattaggt ggcgcttcgg caagctaaag aagaaattca atttccatgc ggcgatcgtc
     1561 acacatcccg atcaggacca ctacaaagga ttccagaagc tgttcgagga aaaacaattc
     1621 tcgttcgaca gggtttatca caacggcatc gccgaacgcg agggagccga tgtgtttggt
     1681 ccgagcgatg cgaccggccg ctacctcacc gatttgaggc caaccgacgt gtcaattcgc
     1741 agcctctatg ctgacgcgga agtgcgggga aggaagctat atccgaagct tgtgcacacg
     1801 gcgctgaccg gcggccgcgt gggcgcggtc gagatgcttt cgacgcggca tggccgtgaa
     1861 caagatggtc gaagctggat gcctggcttt gatcccgatg cgagtccgga acttacgatc
     1921 gagatccttg ggcctgtcgt agagcctgac actcacgaca cgccgcgact ccgttggttc
     1981 ggcgacacgg ttggatctct cggaaaggat ccgggcaaga ccaagaacgg ccactctgtt
     2041 ctgctgcggc tttgctaccg caatttcacc ctgctttttg gaggagatct caatcgccct
     2101 gccgaggatt tcctcctgcg acactatggc gggatcggcg acggaccgcc acttgccgac
     2161 gcggtggaga aggcacgcaa gcggcttcgc tccgacgtca tcaagagctg ccatcacggc
     2221 tccgccgatg tgacggatga gtttctacga gccgtcagtc catttgcatt tggggtttcg
     2281 tcgggcgatg aggagagtca cgtgcatccg cggccggact tgctcggccg tctcggccgt
     2341 cacggacgtg gcgaggcgcc gctcatcctt tgcaccgaga tcttgcgctc cacccgcgag
     2401 acggaagaca aaaaactgct gcgccgactg caagcgctcg acgctcttat cgagattccc
     2461 aacaccccgg aatccgacaa gaagcctctg agaacggaaa gggcggcgct tcagagcaag
     2521 ttggccgaac gcaatgtcga gatttatggc gcaatcacgc tccggaccga tggaaagcac
     2581 ctcgccattg ccttccgaat ggagagagag cgaaagggca agagcccgaa acgttggcaa
     2641 atctattggt tcagtcacga tgagcagcgg ggcttcgttt cagaacacgg tgagtgatgt
     2701 tcgcctgcgc taggccatag ggcgcccagg ctcccccggg gcggttgccc tttccgcgcc
     2761 aagtgcgtag atcagcgcta tgctgagatt tctgaaggcg cgcgcgggcg gcgccaatgg
     2821 ccgcaacggt agggcggcaa gaggcgccgg tcgcggcaag gcggcggtca ccttcgcggc
     2881 ccagctcacc ttggaagagg tgtcgcgccg ttatggcgaa acgctggcgc ttgaccgcgt
     2941 cagcctcgat atcgcgccgt cggagatcct ctgcctgctc gggccttcgg gctgcggcaa
     3001 gtcgaccttg ctcagggtcg ccgcaggcgt cgagcgtcca tcctcggggc ggatcctcct
     3061 cgacggccag gaggtcgcgg gacccgatag cttcgtgccg ccggagaagc gcgggatcgg
     3121 gctgatgttc caggacttcg cgctcttccc gcacctgacc attctcgaca atgtggcgtt
     3181 cggcctcaaa tcgctgacgc gaagcgaagc caagaccgag gcccgcgccg cgctcgagcg
     3241 tgtcggtctc gcgcattatg cgagcgagta cccgcacatt ctctccggcg gcgaacagca
     3301 acgcgtggcg ctggcgcgcg cgatcgcgcc gcggccgagc gtcctcctca tggacgagcc
     3361 gttctcgggg ctcgactcgc gccttcggga aaagatgcgc gaggagacgc tcgccatcct
     3421 gcatgagacg cgcgccaccg cgatcgtcgt cacccatgac gcggaggagg ccatgcgcat
     3481 gggcgatcgc gtggcgctga tgcatcaggg gaaggtcgtg caaaccggaa aggcgctcga
     3541 tctctaccgc gcgcccaagg acatcctcac cgcgcgcacc ttctccgact tgaacgagct
     3601 tgccgcgcgg atcgaggacg gaagtgcttt aaccccgctt ggccgcttcg ccgccaatgg
     3661 cctgcccgat ggcgccgagg ccatcgtttg cgtacgccag cggggggtgc gcctgcttgc
     3721 gccgggggag ggcgtgccgg cccgcattct cgatgcccgc attcttggcg acgtcgcctt
     3781 ggtcgaggtg gccgtccaag ggctcgacgc gccgctcttc gcgcgtgtca gggagagcga
     3841 cgttccgccg caaggtgcgg aagtcggcat cgtcatcgaa accggcgcgg tcctcgtgtt
     3901 cgcgggcggc aatggcaacc ggccggcccc ctgagcgtcg cggatcgctg gcttgcgacg
     3961 gccctgcctt tgctgccata gtgtctccaa gaaaccggag tatgcctgaa ggctgatcgg
     4021 cctcggcgcg atggagattt gtaatgggat tgagctggtc gcacattctg atcgttcttg
     4081 tgctcttcgt gctgctgttc gggcgcggca agatctcgga gctcatgggc gacgtcgccc
     4141 acggcatcaa gagcttcaag aaaggcatgg cggaggacga gcaggcccca gccacggaga
     4201 ccaaagccat cgagcacgag agggccaagc ctgccacgaa gtcggcctcc cgcgccaaag
     4261 ccccgcacaa gcagaaagcg ggctaaccgc ccgcgcgatt ctgccggcaa gtttggggcc
     4321 cgcggccctc ctctgaaggg cgcacatgtt cgatatcggt tggtctgagc ttctcgtcat
     4381 cgccgtggtc gcgatcgtcg tggtcgggcc gaaggagctg cccaagctcc tgcgaacctt
     4441 cggctattac gccggcaagc ttcgccgcgc cgcggccgaa ttccgccgcc agttcgacga
     4501 tgccatgcgc gaggccgagc ttgaggaagt gaagaaggcg atcgagagcg ttcggaccga
     4561 gacggcctcg ctcgacgaga aagcgccact caacaagcgt ttcatgccgc cgacattgga
     4621 ggaaagaccg caactggttg agcccgaggc ggtgaagcct ccgccgaagc cgccgcgaaa
     4681 acgcgccgct ccgaagacca aatcgcccgc caagcgcgcc aagagcggcg ctgaaccttc
     4741 gccatgaccg atcaagacat cgacgcgacc aaggctcctc tgatcgagca cctgatcgag
     4801 ctcaggaaaa ggcttgtctg ggcgctgctt gcgatatttg tcgcctttct tgtctgcttc
     4861 tggttcgcca agccgatcta caatcttctg ctctggccat accggctcgc ggcgggcttg
     4921 gacgctccca tcgagatgat ctacaccgcg ccgcaggaat tcttcttcac ccaggtcaag
     4981 ctcgctcttt tcggcgcgat cttcatcgcc ttccccgtgc tcgcgacgca gatctacatg
     5041 ttcgtggcgc cgggacttta tcgcaacgaa cgccaggcgt tccttccctt tctggtcgcc
     5101 accccgctcc tgttcctgct cggcgccgcg ctcgtctatt tcatggcgat gccgctcgcc
     5161 atgaagttct tcctgtcgat gcagcagacc ggcgaccagc aggtgcagat tcatctcacc
     5221 gcgcgggtga gcgagtatct ctcgctgatc atggcgctga tcctcggctt cggcatctgc
     5281 ttccaattgc ccgtcgttct gacgcttctc gcccgcgcag gcatgatcac cgccgctcag
     5341 ctcaagcgct accgccgcca cgcgattttc gccgtgttcg tggttgccgc cgtgctgacc
     5401 ccgcccgatc cgatcagcca gatcgcgctc gccgtgccga ccgtgctgct ctacgaggcg
     5461 tcgatctatg cggtgaagct cgccgagaag aagcatgccg cctcggccgc cgccgccacg
     5521 gccgccgccg ccacggcctc ggccgcgaag cccgccggca agccggcctg atgaggagga
     5581 atccctccca atacccttcg tcatgcccgg acttgatccg ggcatccatt ccgtttcgtt
     5641 tcagtaagat gaagcgccat gggaatggat tgccgggtca agcccggcaa tgacgagcag
     5701 cgtggagggg cacgcttcgc tttgcccacc tgacgggtta tagaggcccc ctgaatcgct
     5761 gaagcgcaaa agttcacatc gtgctcgaca tcaaatggat ccgcgacaat gaggaggcct
     5821 tcgtcaaggg gctgaccaat cgcggctacg acgatccgcg cgcgacgttg aagcgtctgc
     5881 tgagcctcga cgcgcaacgc cgcgcaacga ttcagaaatt gcaggaggcg caagcgcggc
     5941 gcaacgcggc ctcgaaggag atcggcagcg ccaaggccaa gaaggacgag ccggccgcca
     6001 agcgcctcat ggacgaggtg gcagccctca aggatacgat acaagcgggc gaggccgagg
     6061 agaagcggct tgacggcgag cttcacgcgc tgctcgccac ggttcccaac actccccaat
     6121 cggatgtgcc ggtcggaccc gatgcccgcg ccaacaaggt cctgcgcgag gtcggcaagc
     6181 ccgagacatt cgcgttcacc ccgaagcagc atttcgagct tggcgaggcg ctcgggctca
     6241 tggatttcga gaccgccgcc aagctctcgg gcgcgcgctt cgtggtgctg aaaggggcac
     6301 tcgccaggct cgagcgcgcg cttgcctcct tcatgctcga cctgcatacg agcgagcacg
     6361 gctatatcga gatcaatccg ccgattctcg ttcgcgacga cgccatgttc ggcacggcgc
     6421 aactgccgaa attcgccgag gatcaattca ggactgggcg gccagagaca gtggatgatc
     6481 tattgagccg actaaagcct gtaatcgaag gcatctttgc tgagcaaaaa cctctcattg
     6541 aaggcagcga tataaggcgg gtcgaggcac tcaaaatgag ggaattgctc gctgaaaggt
     6601 taggctcgtg gacggaggag ctttcagatg cacagggcat ccctcgcacg gattattggg
     6661 aggacctcgc tcgggaggcc gcgcgaagat atctgaacct ctggctcatt ccaacggcgg
     6721 aagtgccgct gaccaatctc gtgcgcgacg acatcgtcga tgaagcgagc ctgccgctgc
     6781 gcgtcaccgc ctggacgccg tgcttccgcg ccgaagccgg cgccgccggc aaggacacgc
     6841 gcggcatgat ccgccagcat caattctcga aggtcgagct cgtttccatc accacgccgg
     6901 agcaatcggc cgaggagcac gagcgcatga caacatccgc cgagacggtc ttgaaacgac
     6961 tcgaacttcc gttcaggacc gtcgtgctct cgacgggcga catgggcttc gcgtcgcaga
     7021 agacctatga catcgaggtg tggcttcccg ggcaggcggc ctatcgcgag atttcctcct
     7081 gctcgaattg cggcgacttt caggcccggc gcatgaatgc gcgctaccgg ccgaagggcg
     7141 agaagcagcc gcggctggtg cacacgctga acggctcggg ggttgccgtg ggccgcgcgc
     7201 tgatcgcggt gctcgaaaat taccaggaat ccgacggctc gatccgtatc cccgaagcgt
     7261 tgcagcccta tatgggcggc ctgaccgaga tcgcggcgcc gccgaagtga cgcgagcccg
     7321 ggcacagcca agctgcactt tccgcacccg gtgctgacaa tcctcggcta tctttgatat
     7381 cgccaccgag cgggtggaat cgggctatgg catccgcgca cgaccagatc ggtctgatca
     7441 tgcagctcag gcgtcgcggc atccgcgaca atgatgtgct gcgcgccatc gagcgagtgc
     7501 cgcgcgatct gttcgtcgat cccgctttcg ccgaccacgc ctaccacgac atcgcgcttc
     7561 ccatcgattg cggccagacc atctcccagc cttacgtggt cgccttcatg accgagaggc
     7621 tcgagctcga cgcgcggcac aaggtgctgg agatcggcac gggctcaggc taccaggccg
     7681 cggtgctcgc ccatctttgc cggcgggtct ataccgtcga gcgctggcgg gagttgcaga
     7741 aggcggccga gcggcgcttc gccaggctcg gcatcaccaa cgtgaccacg atcatcggcg
     7801 atggctggct gggctggccg ccgcaagccc cgttcgatcg catcatcgtg accgccgcgg
     7861 cgaaagaagc gccgccggcg ctcatcgagc agctgaaggt cggcggccgc atgatcctcc
     7921 ctttgggcga gacccgcgac acccagcagc tcgtgcagat cgacaagacc gaggcggggc
     7981 tcgccgagac accgttgctg ccggtccggt tcgtgccgct cgtgcacggc agggcaggcg
     8041 tcgctgcccg acagaaggac acgcgcacca tagagtccga tgacgaatag gcacctctag
     8101 cccgaatctc ccgcgccaaa cccagggcta acagcttgtt aaccgcatcc gcgctttact
     8161 tggcggcgac gggtatcttt aggggcgtat gggggaatgc ggcgggcgtc gttcttgtcg
     8221 gactcgggcc ggggcgcctg cttggcgctc atggcggcgt gcgtgggcct gaccgccgca
     8281 gggtgcagct caaccgcatc gcgcttcgat tttccgtcgt tcagctccac cgcctctgac
     8341 agcagcggca atgccgacgt cgcgacgact tcggcgctgc ccgttcccga acagtcggtt
     8401 tacagccagg ggtccggggc cgatgcgcgg ctccagcgga ccgccttgcc gtccccggcc
     8461 tattcgccgg ccccgaacag acggatgggc gcaactcccg caccctatgc gcaatccccc
     8521 gcgaagggac accgcatcaa ggtcgcaaag ggcgatacgc tttccagcgt atcgcggcgc
     8581 tacggcgttc cggtcgaggc gatcgtcagc gccaacaatc tgcccgacgg gcgtcttgcc
     8641 gtaggtcagg agctggtctt gccgggcgtc acggccgcca aagcggccgc cgtcgccaag
     8701 cctgcgccca acgtgcgcgt ggtgaagaca gagccgatcg ccgcgccgaa aggcacatcg
     8761 ctcgccgagg aagaagcggc cgaggctgcc ggcaacaaga agcagaacaa ggcgagcgag
     8821 attgccgcct cgcccgagac caccggcccg ggtgcccagc gcgtggccgg cgtcgatcag
     8881 ctgccggcgc ccgatcccat gtcgggcaac agcttccgct ggccggtcca gggccgcatc
     8941 atcgccggct ttggcacgcg ccccgatggc ggccacaatg acggcatcga cgtcgcggtc
     9001 ccgcaaggca cctcggtcaa agccgccgag aacggcgtcg tcgcctattc cggcaacgag
     9061 ctcaagggct acggcaatct cgtgctggtc cggcacgcca acaattgggt gtcggcctac
     9121 gccaataacg ccgaacttct cgtcaagcgg ggcgacaagg tgcgccgcgg ccagatcatc
     9181 gccaaggccg gcgccaccgg cgcggtcagc cagccgcagg tgcatttcga gctgcgcaag
     9241 ggatcgcgcc ccgtcgaccc caccaaatat atgagcaatc aggcggccaa cgccgaatag
     9301 ccgctgaccg cccggccgct agagccgcgt gccgagcctg ccggcgaggt cctgcacata
     9361 ttgccaggcg acgcgccccg agcggcttcc gcgcgctcgc gcccaagcga gcgcctcatc
     9421 gcgcagaacc gcgtccgaaa ccgcgatctg gtagtgatgg acgtagccct gcaccatggc
     9481 gagataatcg tcctggctgc aattgtggaa gccgatccat aagccgaagc gatccgacag
     9541 cgaaagcttc tcctcgaccg cctctccggg attgatggcg gtcgagcgct cgttctcgac
     9601 catctcccgt ggcatcagat ggcggcggtt cgaggtggca tagaacacca cattggcggg
     9661 gcgcccttcg atgccaccct cgagcacggc cttcagcgat ttgtacgacg tgtcgagcgc
     9721 ctcaaaggac aggtcgtcgc aaaaaaggat gaagcagcga tccgggttcg cgcgcaaatg
     9781 cgaaagcgcc cgcggcaatg agccgagatc ctcgcggtgg atttccacga gcttgagccc
     9841 ggcaatctcg ttatgaatgg ccttgaccag cgcgctcttg cccatgccgc gtgcacccca
     9901 gagcagcgcg ttgttggcgg gaagcccccg ggcgaagcgg agcgtattgg caagaagcgt
     9961 gtcgcgcatg cggtcgattc ccttgagcag cgacagcggc acgcg
//
LOCUS       DASUQP010000252         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_534137,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000252 DASUQP010000000
VERSION     DASUQP010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534137"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..626
                     /gene="bfr"
                     /locus_tag="VFY74_11545"
     CDS             129..626
                     /gene="bfr"
                     /locus_tag="VFY74_11545"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX5932362.1"
                     /translation="MKGNKRVIEYLNKALTHELTAVNQYWLHYRLLDDWGFTKLAKKE
                     RAESIEEMQHADKLVNRIIFLEGFPNMQHLNPLMIGQNVKEVLECDLKAEYSARALYM
                     EARAACREEEDYVTMDLFEELLGDEEGHINFIETQLNLLKDIGKENYGQLNAHSADEV
                     PEEGH"
     gene            629..1297
                     /locus_tag="VFY74_11550"
     CDS             629..1297
                     /locus_tag="VFY74_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5932363.1"
                     /translation="MLSIDGPRLPPAAGGAARQLVVFLHGYGADGNDLIGLGREWAKL
                     LPHAAFVSPHAPEPCGAAPMGRQWFNLTFRDAGELLRGVTQAAPALDAFLDAELKRHD
                     LGARALALVGFSQGTMLALGVGLKRRPAPAAIVGYSGALANVEALPNDQAAAPAILLV
                     HGDVDAVIPVDAMFIAREQLARAGLAVEWHVAEGIGHGIDPHGLNLGGTFLRQAFAAA
                     QAIP"
     gene            complement(1421..1843)
                     /locus_tag="VFY74_11555"
     CDS             complement(1421..1843)
                     /locus_tag="VFY74_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5932364.1"
                     /translation="MTERSKSGDGRIIPALRYRDAAAAIDWLCLAFGFERKMVVPGDG
                     GTIAHAELTLGNGMIMIGNVETKYGSLVKAPVPPDYVNTQGIYVVVEDADAHYARAKE
                     AGAVILLDITTQDYGGRDYTARDPEGHVWTFGTYNPWA"
     gene            1981..2547
                     /locus_tag="VFY74_11560"
     CDS             1981..2547
                     /locus_tag="VFY74_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5932365.1"
                     /translation="MNVATASAAPGTFPALVLNADFRPLSYYPLSLWGWQDAVKAVFL
                     DRVNIVSEYERAVRSPSIEMKLPSVVSLKTYVRPALYPAFTRFNVFLRDKFTCQYCGD
                     DQDLTFDHLTPRSRGGMTRWENVTTACAPCNLSKGGKLPNEAKMYPSQKPYRPTVFEL
                     HRNGRLFPPNYLHESWLDYLYWDTELEP"
     gene            complement(2560..3054)
                     /locus_tag="VFY74_11565"
     CDS             complement(2560..3054)
                     /locus_tag="VFY74_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX5932366.1"
                     /translation="MTASVLVLAIASVTIVAALAFEHLGGYAACPLCLEQRYAYYFAV
                     PASALALLLARGEATGIARILLALIALAFIANAVFGIYHAGIEWKWWEGPGACAGGFD
                     LEWREGGVVETPVIRCDEASWRFLGLSFAGWNAAISAALAGIAAYGAAKGARRTADAA
                     DHKF"
     gene            complement(3104..3685)
                     /locus_tag="VFY74_11570"
     CDS             complement(3104..3685)
                     /locus_tag="VFY74_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX5932367.1"
                     /translation="MLRNMYDRVIALSESRHALPTLAVVSFAESSFFPIPPDVILVPM
                     ALARPEKARLYALVCTIASVAGGVLGYAIGAFLYDTLGKWLISLYGYGDSVEAFRAAY
                     AEWGAWIILIKGMTPIPYKIVTIASGFAGYDFFMFVVLSLITRGARFFLEAELLRIYG
                     EPIRGFIEKRLTLVTTGFLAAIVGGFLIAKYAF"
     gene            3790..3876
                     /locus_tag="VFY74_11575"
     tRNA            3790..3876
                     /locus_tag="VFY74_11575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3824..3826,aa:Leu,seq:caa)
     gene            4051..4656
                     /locus_tag="VFY74_11580"
     CDS             4051..4656
                     /locus_tag="VFY74_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5932368.1"
                     /translation="MLTDTRVRNAKSTQRAIKLSDAGGLYLLIQPHGSKLWRMAYRFV
                     GKQKTLAFGVYPTVGLQDAREKRDAAKRLLGKGIDPSAQRRLERQAARTLGSTFKDVA
                     DELMLKLEREGRADATLTKKRWLLQSAYPSLGHRPIAEITAPELLSVLRKVEERGCYE
                     TARRLRSTLGTVRYRPGRARPGSRLARCADLAQSHSPAGYR"
BASE COUNT          902 a         1522 c         1474 g          862 t
ORIGIN      
        1 tcgcctacga tgagaagggg tcaagctcgg gctcgacctt cgcccatcgc ccgccggtcg
       61 agggtaccta gcttcggcgc aaagaacggg ccatgatcca cctaacaggt tgagaggagg
      121 acagaagaat gaagggcaat aagcgggtca tcgagtatct caacaaggcg ctgacccacg
      181 aactcaccgc cgtcaatcaa tactggctgc attaccggct gctcgatgat tggggattta
      241 ccaagctcgc caagaaagag cgggcggaat cgatcgagga gatgcagcac gccgacaagc
      301 tcgtgaaccg catcatcttc ctcgaaggct tccccaacat gcagcatctc aatccgctga
      361 tgatcgggca gaacgtcaag gaagtcctcg aatgcgacct caaggccgaa tactcggctc
      421 gcgccctcta tatggaggca cgcgccgcct gccgcgagga ggaggattat gtgacgatgg
      481 atctgttcga ggagctgctc ggcgacgagg aaggccacat caacttcatc gagacgcagc
      541 tcaacctgct caaggacatc ggcaaggaga attacgggca gctcaatgcc cattccgccg
      601 atgaggttcc cgaggagggg cactaggctt gctgtcgatc gacggaccgc gtcttccgcc
      661 ggcggcaggc ggtgcggcgc gccagctcgt cgtcttcctg catggctacg gcgccgacgg
      721 gaacgacctg atcggcctcg ggcgcgaatg ggcgaagctc ctgccccatg ccgccttcgt
      781 ctcgccccat gcgcccgagc cttgcggtgc cgcgcccatg ggccggcaat ggttcaacct
      841 caccttccgc gacgccggcg agctcctccg cggcgtcacg caggccgcgc ccgcgctcga
      901 tgcctttctc gatgccgagc tcaaacggca tgacctcgga gcgcgcgcgc ttgcgctcgt
      961 cggtttcagc cagggcacga tgctggcgct cggcgtcggt ctcaagcgac ggcccgcgcc
     1021 ggccgcgatc gtgggctatt ccggcgcgct ggcaaatgtc gaggcactgc caaacgacca
     1081 agccgcggcg cccgcgatcc tgctcgttca tggcgatgtt gacgcggtca ttcccgtgga
     1141 cgcgatgttc atcgcccgcg agcagctcgc gcgggcgggg ctggccgtgg agtggcacgt
     1201 tgcggaaggg atcggtcacg ggatcgaccc gcacgggctt aatctcggcg gcacctttct
     1261 ccgccaggct tttgccgccg ctcaagccat tccttagccg cgcccgcgat gcgggaagcg
     1321 tccgcttccg aagtaacgat ccgacatcgc accacacgga gggaatattc aggactgaag
     1381 gccgaacagg cagcgccgcg cttatcagtg ttgggcccag ctaggcccag ggattgtagg
     1441 tcccgaaggt ccagacgtga ccttcgggat cgcgtgccgt gtagtcgcgc ccgccatagt
     1501 cttgcgtcgt gatgtcgagc aggatcaccg cgcccgcttc cttcgcccgc gcgtagtgcg
     1561 cgtcggcatc ctcgaccacc acatagatgc cttgcgtgtt gacatagtcg gggggcacag
     1621 gcgctttgac cagactgcca tatttcgtct cgacattgcc gatcatgatc atgccgttgc
     1681 cgagggtgag ttcggcatgc gcgatggtgc cgccatcgcc tggcaccacc attttccgct
     1741 cgaatccaaa tgccaggcac aaccagtcga tggcggccgc ggcgtcccga tagcgcagcg
     1801 cggggatgat gcggccatcg ccgcttttgc tgcgttcagt cattttcttc tccaccgtaa
     1861 gggtccgcac gcaacagcct atggcccccc ttcacattcc tgacatgccc cgatagtatc
     1921 cggcccggca aggaggatgg tccccgcgtg cgacgggaat agggtaagga atctcgcgcc
     1981 ttgaacgtcg ccaccgcatc ggccgccccc ggcacgtttc ccgcgctggt gctgaacgcc
     2041 gatttccggc cgctgtccta ctacccgctc tcgctgtggg gctggcagga cgcggtgaag
     2101 gcggtattcc tcgaccgcgt caacatcgtc tcggaatacg agcgtgccgt gcgctcccct
     2161 tccatcgaga tgaagctacc gagcgtcgtc tcgctcaaga cttacgtgcg gcccgcgctc
     2221 tatccggcct tcacgcggtt caacgtgttc ctgcgcgaca aattcacctg ccaatattgc
     2281 ggcgacgacc aggacctgac cttcgaccat ctaacgccgc gctcgcgcgg gggcatgacg
     2341 cgctgggaga acgtcaccac ggcgtgcgcg ccttgcaatc tctccaaagg cggcaagctg
     2401 cccaacgaag cgaagatgta tccgtcgcag aagccctacc gtcccacggt gttcgagctt
     2461 caccgcaacg ggcgcctgtt cccgccgaac tatctgcatg agagctggct cgattatctc
     2521 tactgggata ccgagctcga gccgtaagcc gtatccccgt cagaacttgt ggtcagccgc
     2581 gtcagccgtt cggcgcgcgc cctttgctgc gccgtaggcg gcaatgccgg cgagcgcggc
     2641 ggagatcgcc gcgttccaac ccgcgaagga cagtcccaga aagcgccacg aggcttcgtc
     2701 gcagcgaatg acgggagtct cgacgacgcc gccctcgcgc cattcgaggt cgaagcctcc
     2761 ggcgcatgcg ccgggacctt cccaccattt ccattcgatg ccggcgtgat agatgccgaa
     2821 gacggcattg gcgatgaagg caagcgcaat gagagcgagc aggatccgcg cgatgccggt
     2881 tgcctcgcca cgcgcgagga gcagcgcgag cgcgctcgcc ggcacggcga aataataggc
     2941 gtagcgctgc tcgaggcaga gcgggcaagc ggcgtaaccg ccgagatgct cgaaggcgag
     3001 cgccgcgaca atcgtcacgc tcgcgatcgc gagcacgagc acactcgcgg tcacggccaa
     3061 tcttggcgtc ctcgccaagc tcgggcttgc cgttgcgtcc atcctagaaa gcatatttag
     3121 cgatcaggaa gccgccgaca atggctgcga gaaagccggt ggtgacgagc gtcagccgct
     3181 tctcgatgaa gccgcggatc ggctcgccat agatgcggag cagctccgcc tcgagaaaga
     3241 accgcgcccc gcgggtgatg agggacagga ccacaaacat aaaaaagtcg tagccggcaa
     3301 agccggacgc gatggtcacg atcttgtagg ggatcggcgt catgcccttg atcaggatga
     3361 tccacgcgcc ccattcggcg taagccgcac ggaaggcctc gacgctgtcg ccatagccgt
     3421 agagcgagat cagccatttg ccgagcgtgt cgtagaggaa ggcgccgatg gcatagccga
     3481 ggacgccgcc cgcgaccgag gcgatggtgc agacgagcgc ataaagcctc gccttttccg
     3541 gcctggcgag cgccatgggc accaaaatca cgtcgggcgg gatcggaaag aacgagcttt
     3601 cggcgaagga gaccacggca agcgtgggca acgcgtggcg gctctcggag agcgcgatga
     3661 ccctgtcgta catattgcgc agcatcgccg cttgattagc cgggcggcac ggtcttgtcc
     3721 atcttgtccg gggtcccgcc gcgtgccccg gttgaccgcg ccgcgcgctt accccatgtt
     3781 ccgcccctag ccccagtggc ggaattggta gacgcggcag actcaaaatc tgttgcccgc
     3841 aagggcgtgc tggttcgagt ccggcctggg gcaccacctt caatgttcgc cgttgttcgc
     3901 ctctatcctt ccacggtcgg attcagctta catagccacg tattttttgc agttaagtgt
     3961 tcgcagcgat gtgcggtgac gtattggcat ccgagcaatt tgttggtacc tgagttggta
     4021 ttcctcgatt ctgattcata ggataccaac atgctcaccg acactcgtgt gcgcaacgcc
     4081 aagtctactc agcgagcgat caagctctcg gatgctggcg gcctttatct gctgatccag
     4141 ccacacggca gcaagctttg gcgcatggcc tacaggttcg tgggcaagca aaagacctta
     4201 gcctttggtg tctatccgac agtaggtttg caggacgcgc gcgagaagcg tgatgcggcc
     4261 aaacgattgc tgggcaaagg cattgatcct tcggcccagc gccgacttga aaggcaagcc
     4321 gcccggacgc tcggcagcac attcaaggac gttgccgacg agctaatgtt gaagcttgaa
     4381 agggaaggcc gcgcggatgc gaccctcaca aaaaagcgtt ggttgctcca atctgcttat
     4441 ccgtcgcttg gtcaccgccc aattgccgag atcacagcgc cggaactgct gtcggttctg
     4501 cgtaaagttg aggagcgggg ctgctatgaa accgcccggc ggctgcgcag caccttgggg
     4561 accgttcgct acaggccggg ccgagcgcga cccggcagcc gacttgcgcg gtgcgctgac
     4621 ctcgcccaaa gtcactcacc ggccggctat cgttgacccg gcggatattg gagcattgct
     4681 cagggctatc gacggctacg ccggtcaccc ggtaactaag tcggctttgc gattggcccc
     4741 tctggtgttt gtgcgccctg
//
LOCUS       DASUQP010000253        15203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1486959, whole genome shotgun sequence.
ACCESSION   DASUQP010000253 DASUQP010000000
VERSION     DASUQP010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15203
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486959"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFY74_11585"
     CDS             <1..333
                     /locus_tag="VFY74_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5932369.1"
                     /translation="EREPYATVKAFWRSCGAPDFAPVEALDVPRLHEGLDWLMQWDVR
                     DAKDGLARPMLALAARDDAVVPAAMSEAIWRKPGLVWSPDGGHVLPLRHPQWCARHVL
                     EFARSLPA"
     gene            299..1084
                     /locus_tag="VFY74_11590"
     CDS             299..1084
                     /locus_tag="VFY74_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5932370.1"
                     /translation="MSLNSPVLSRRSAEVAASFGARAESYELHAGLQRAIAGRLARFL
                     PAFERPRVLELGCGTGLFSRHLVRRYPQGYFVLTDVAPAMIAECRRNLTPSGAAHISY
                     EVMDAGEAGGHAGLDLIVSSMTLHWLADPVLSLERLRRFLGPGGFLLYATLGPESFAE
                     WRAALSSEGLPSGLADIPSLPGVVEEERLTPDTDTLSFLRRMKGVGGLTPREGYAPLS
                     PGALRRAIRATDMRFGGRVTWHIVYGALKAAEASASRPAATPS"
     gene            complement(1024..2391)
                     /gene="bioA"
                     /locus_tag="VFY74_11595"
     CDS             complement(1024..2391)
                     /gene="bioA"
                     /locus_tag="VFY74_11595"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX5932371.1"
                     /translation="MNDDRQIPRQGASTAAEVLARDQRHIWHPYTQHGTDNAPIVIAR
                     AKGASLFDAGGREILDIVSSWWTCTHGHSHPKLNAALAEQASRFEHVMFAGFTHAPAA
                     DLATALAGILPGDLNRVFFSDDGSTSVEVALKIAYQYWINRGETRRRGLIAFDGGYHG
                     DTLGAMSVGRGSQFFTPFRDLMCEVTVLPYPATFEGDDAVDEREAGALSALAATLTDR
                     RTGVAALIVEPLLQGAGGMRLCRPSFLKRLVESAREAGVLIIFDEVATGFGRTGTLFA
                     MEQAEVVPDLVCLSKGLTAGYMPLGATVVREALFEAFLGESFERALPHGHSFTANPLA
                     CGVGLASLALFGEEKTLERIARISRSHRAFMAELAARSDVTRPRLIGSILAFDVKEAG
                     AYQSAASRRLCDWYLARGLNIRPLGSTVYLMPPYCITDEELGRTYEGVAAGLDALASA
                     ALSAP"
     gene            complement(2388..3023)
                     /gene="bioD"
                     /locus_tag="VFY74_11600"
     CDS             complement(2388..3023)
                     /gene="bioD"
                     /locus_tag="VFY74_11600"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX5932372.1"
                     /translation="MVQGFFVTGTDTDVGKTVVAAWLVARLGACYWKPVQAGTHPETD
                     SATVRRLSGANPDRILPEAYVLAEPIAPHEAALREGIGIDMAKLVPPPCNRPLVAEGA
                     GGLMVPLNDQSFVIDLARELDLPLILVARSTLGTINHTLLSLEAIRRRGLALAGVVIN
                     GPETPHNRAAIERYGRVEVIAEIPWLEQVTPDALMAIEPEIDLAKLARSAA"
     gene            3093..4685
                     /locus_tag="VFY74_11605"
     CDS             3093..4685
                     /locus_tag="VFY74_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5932373.1"
                     /translation="MDATAPFGRHSGPMRRLLAVVSLALFAAFAPQGLAAKTLRMAYD
                     ADPTSLDPHEQLSGATLQMSHLLFDPLIRFRQDLSIEPRLAKSFEKIDERTTRFHLRE
                     GVKFQSGRTLSAEDVVWTFNRLKQSADFKALFEPFSEARAVDDLTVDLVTKGPYPLVL
                     NLATYIFPMDRVFYSGPDERGRPKDAIVKHGASFASTHASGTGPFIVTEREQGVRLEL
                     ERFADYWDEGSPGNVDEIVFTPIKEPATRVAALLAGDVDFIAPVPPTDLARLKQASCC
                     TLITMPSTRVLTFELNQERVLAFKDSRVRLAMVLAVNGQGIAEKIMRGLGTAAGQLSP
                     PGYAGHDPELTPRYDLAKAKALMKEAGYEDGFSVTMMAPNNRYIEDARVAEAVAAMLA
                     KINIRVDLQTMPKAQYWQRFDERAAGIMMIGWQSDTQDSANFYEFLVMTPDKDKGYGQ
                     YNAGSYSNPEIDRLTLETQAMTDSDARAAVLRKIERLIYDDAALMPLHWQHLSWAARN
                     GVKIDAVVNVIDMPYLGDLVIE"
     gene            4722..5258
                     /locus_tag="VFY74_11610"
     CDS             4722..5258
                     /locus_tag="VFY74_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5932374.1"
                     /translation="MFKKIIIGLAALILIFVAIVAMQPSSYRIERTAKIAAPQADLFG
                     QVNDFHKWETWSPWAKLDPAAKITFEGPDSGQGAVMNWAGNDKVGEGKLTIVESRPNE
                     LVKVKVDFVEPFEGTNTSEFQFKPEGDQTAVTWIMSGEHNFIGKAFCLVMNGEKMMGD
                     DIEKGLAQMKSAAKTASN"
     gene            5422..6009
                     /locus_tag="VFY74_11615"
     CDS             5422..6009
                     /locus_tag="VFY74_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932375.1"
                     /translation="MRTRAVTLMAGVGILTGLISSLPSPLPDLRLVEPEFLINAKNVP
                     LHAGIAFGAGIAIMLWLWVTRDLGKCLLAVALTIVGWLVAANTANDVISVTVSSDLFG
                     TAEGAKANREVVGCVLAGLIAGAIGAGLTAFGAGIAADPIRRAKAWIPIVATGALFGL
                     LLYPAAHWNAIALLFVPWQAAVAGLIAHALTRPAA"
     gene            6026..7018
                     /locus_tag="VFY74_11620"
     CDS             6026..7018
                     /locus_tag="VFY74_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5932376.1"
                     /translation="MSLIAAGLSFAARRFAQAALVIFAILVIAFAVRAALGDPLREIM
                     GEAVPDAERAALRQKLGLDAPWPVQLARYLGGAVQGDLGTSTIYKRPTLAIILAKFPA
                     SFELVLGASVIVFLLSLPAGIYCAVRPRSLGARLTLNLSVLGISIPVFLTGIFLITVF
                     SVWLGWLPAFGRGDTVRLGPWETGLVTRDGLAHLILPSLALSSIMLPLFIRLVRGAML
                     EELGHDYVRTARAKGASPLRVWLAHALRNALLPLTAIGGLQVGTLIAYTLLTETVFQW
                     PGMGFLFLEAVTRADIPLITTYLVFVGLIFVVTNTLVDIVSLMLDPRVSLEGTR"
     gene            7015..7935
                     /locus_tag="VFY74_11625"
     CDS             7015..7935
                     /locus_tag="VFY74_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5932377.1"
                     /translation="MSTEAVPNRRSALRVLAAEHPGGVIAAIVLGAIALAAAAAPLIA
                     PQDPFDLASFDVLDASLPPSFLEASDPRFLLGTDAQGRDLLSAILFGTRISLSVGLLA
                     VVIAAAVGVPLGLLAGYAGGRVDTVVTRAADIQLSLSTLMMAIIALALFRAGFGGESL
                     GRFAVPLLAVVIGLAEWPYLARTARAATQVEAAKDYVRAARALGASTPRLLRHHILPN
                     IASPLIVVATTQIAGAIMAEAALSFLGLGMPVTKPSLGTLIRSGYDLMFAGAWWVTVI
                     PGLVLIGLLVSVNVLGDALRDALNPRRRSA"
     gene            8070..9065
                     /gene="glpX"
                     /locus_tag="VFY74_11630"
     CDS             8070..9065
                     /gene="glpX"
                     /locus_tag="VFY74_11630"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5932378.1"
                     /translation="MTTSGAANPELVFSLRRVTEAAACAAFSWIGRGDSLDAGRAALD
                     AMRAALAEVDIDAVVAIGEATRGEAPQPQRGERLGHPGSAFKADVAVDPVEGTSYLVR
                     GQTNALAVLAVAARGAMMNPGPAFYMEKFVASAPALGKIDPIWPVEKKLTTLADVLNK
                     DVSDLTVFVLEKPRHRELVDRILAAGARVALYPAGDVAGALMAAIPGSGIDALMGTGG
                     TPEGVMSACAIRAIGGEFLARLDPQLQTETKAVKDAGIDTAKWYQRDEIITSDDVFFC
                     ATGITTGLMLEGVERSKSHYKVQTMMITGASGERQILTSYMPIERMASIAARDVA"
     gene            9095..10024
                     /locus_tag="VFY74_11635"
     CDS             9095..10024
                     /locus_tag="VFY74_11635"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005655.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEX5932379.1"
                     /translation="MSKRVEHPIILGIVGDSASGKSTLSVGVAQILGEEHCTVICTDD
                     YHRYDRSARAALGLSALDPRANYIDVLEQHLRLLRRGEAILKPVYDHRHGTFAPPEYV
                     KPKAFVIAEGLLGYATRGMRECYDVKIYLDPEEELRVKWKMHRDTTKRGYTEEQVLAQ
                     LARREIDSPNFIQPQRTFADIVIRFQQPSADANDSDTHLDVRHILRPTLPHPDFTPLF
                     DGSSNAGLNLELARDRDGKPVDVLEINGNISDKRAERIEDLLWNLIPEAGHLRDEVGR
                     IDIANGAKSHPLALTQLLVGYHAVKAAMGVRAD"
     gene            10094..10303
                     /locus_tag="VFY74_11640"
     CDS             10094..10303
                     /locus_tag="VFY74_11640"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX5932380.1"
                     /translation="MANEFARKMRKSMTRQEFKLWRHLRELRQLGFHFRRQTPTKNFI
                     VDFACYYPRVIVELDGSQHRRNANR"
     gene            10351..10488
                     /locus_tag="VFY74_11645"
     CDS             10351..10488
                     /locus_tag="VFY74_11645"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932381.1"
                     /translation="MRIWNNEVDTNLEGVFDRILHALGTGRPPTPAAHGRRRTSPRGG
                     G"
     gene            10697..12730
                     /gene="tkt"
                     /locus_tag="VFY74_11650"
     CDS             10697..12730
                     /gene="tkt"
                     /locus_tag="VFY74_11650"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5932382.1"
                     /translation="MDTIEPTTDLIQGQALPEASHRAMANAIRALAMDAVQKADSGHP
                     GMPMGMADVAVVLFTRFLKFDAAHPDWPDRDRFVLSAGHGSMLLYALLYLTGYPKMEL
                     EELKNFRQLHSRTAGHPEYGHAPGIETTTGPLAQGLGNAVGMALAERLLNRRYGDGIV
                     NHYTYCLAGDGCLMEGLSEEAISLAGHLKLGRLIVLFDDNHVSIDGPTSLAVSDDQIE
                     RFKASHWHTARIDGHDPEAIALAIENAKQVTDRPSLIACRTVIGYGAPNKAGTAATHG
                     SPLGEEEVKGAREKLGWRHQPFEVPKPILARWRALGSRNRAAYDAWNAAAKRLDASAR
                     PGLTDPIDDKVKAAIVTAMGAIKSDFSREAAKLATRQSSQKVLEKLLPVIPGLIGGSA
                     DLTGSNGTLTKLHTVVKPGDFSGDYIHYGVREHGMAAAMNGMALHGGIVPYGGTFLVF
                     TDYCRPSIRLSALMQQRVIYVMTHDSIGLGEDGPTHQPIEHLAALRAMPNLNVMRPAD
                     QVECAECWELALLAKTTPTILALTRQSVPLLRTASTGDNLSAKGAYVLIEPSERRDVT
                     LIGTGSELALAVEAAKALEGNGIRAAVVSMPCWELFEKQSASYRDSVLGSAPRVAVEA
                     AGPFGWSRWLGPDGAFIGMQGFGASAPQQHLYKHFGITAEAVAAAARQLIEKR"
     gene            12739..13749
                     /gene="gap"
                     /locus_tag="VFY74_11655"
     CDS             12739..13749
                     /gene="gap"
                     /locus_tag="VFY74_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024279732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5932383.1"
                     /translation="MSNKRVAINGFGRIGRLTFRAYLESGRNDLDFVAINDLGSAEMN
                     AHLLQYDSVHGRFPGRIDVVDGHLKTNGQDVAVLSQATPEKLPWRDLGVDIVMECTGR
                     FTDREGAAKHLTAGAKRVLVSAPCSGADLTVVFGVNHEALTQEHHVVSNASCTTNCLA
                     PVAKVLNQAIGIKHGYMTTVHAYTGDQRPVDTLHKDPYRARASAINLIPSSTGAAKAV
                     GLVLPELKGKLDGASIRVPVPNVSLVDLTFQAARPTTAEEINQAVIKAANGPLKGILG
                     VNAKPLVSTDFNHDPLSSIFDLTGTHVVDDTFCRVVAWYDNEWGFSNRMSDTAVAMGR
                     LL"
     gene            13746..15017
                     /locus_tag="VFY74_11660"
     CDS             13746..15017
                     /locus_tag="VFY74_11660"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5932384.1"
                     /translation="MNETKPAAQKPGAGSSLDLSRIRTIEGIDVRGKRVLIRVDFNVP
                     IENGVVADATRLERVLPTIARLAQAGAKVVVLSHLGRPKDGPSVDTSLRPVALKMREL
                     MPGTKVHFIGDCVGEEPRRGLAALKPGEIAVLENVRFYPGEEKNDPQFARRIAEHGDL
                     YVNDAFSTAHRAHATVDVIADLLPSHAGLTMMAEIAALGRALENPERPVMGIVGGAKV
                     STKIEVLTHLVSRMDVLVVGGGMANTFLLAQGMKVGASLCEPDFVPTAKDVMARAARS
                     GCEIVLPSDVVIANALKEGVDWRVCPVGEVPDGALILDFGPESIAALEQRLKGVRTVL
                     WNGPLGAFETAPFGEGTFALAREVARLTEAGKLVSVAGGGDTVRALKEAGAASGFTYV
                     STAGGAFLEWLGGHELPAVRALARSGARSAI"
BASE COUNT         2583 a         5113 c         4823 g         2684 t
ORIGIN      
        1 gagcgcgagc cttatgccac ggtgaaagcc ttttggcgct cttgcggcgc gccggacttt
       61 gcgccggtgg aagcgctcga tgttccccgc ctccatgaag ggctcgattg gttgatgcaa
      121 tgggacgtgc gagacgctaa agacgggctc gcgcgcccca tgctagcgct tgccgctcgc
      181 gacgatgccg ttgtcccggc ggccatgagc gaggcgatat ggcggaaacc ggggctcgtc
      241 tggtcgccgg acggcggcca tgtccttccc ttgaggcacc cgcaatggtg cgcgcgtcat
      301 gtccttgaat tcgcccgttc tctcccggcg tagcgcggag gtcgccgcaa gcttcggcgc
      361 gcgcgcggag agctatgaac ttcatgccgg gctccagcgc gccatcgctg ggaggctcgc
      421 gcggtttctg cccgcgttcg aacggccccg cgtgctcgag cttggctgcg gcaccggcct
      481 gttcagccgc catctcgtgc gccgctaccc gcaagggtac tttgttttga cagacgtcgc
      541 cccggccatg atcgctgaat gtcggcggaa ccttacgccc tcgggcgcag cccatatcag
      601 ctacgaggtg atggacgcgg gcgaggcagg cggccatgcc gggctcgacc tgatcgtgtc
      661 gagcatgacg cttcactggc tggccgatcc ggtcttgagc ctcgaacgcc tgcgccggtt
      721 ccttggccct ggcggattcc tgctctatgc aaccctcggg ccggaaagct tcgccgagtg
      781 gcgcgccgcg ctttcaagcg aaggacttcc gagcgggctt gccgacattc catcgcttcc
      841 cggcgtggtc gaagaggagc ggcttacgcc cgataccgac acgctctcgt tcctccgccg
      901 catgaagggg gtcggcggat tgacgccgcg cgaaggctat gcgccgcttt cgcccggggc
      961 gctccgccgc gccatccgtg cgaccgacat gcgcttcggc ggccgcgtca cctggcacat
     1021 cgtctatggc gcgcttaagg ccgccgaggc gagcgcatcg aggcccgcgg ccactccctc
     1081 ataggtgcgc cccagctcct catcggtgat gcagtaaggg ggcatgagat agaccgtcga
     1141 tccgagcggt ctgatgttga gaccgcgggc gagataccag tcgcagagac gccggctcgc
     1201 ggccgactga taggcgcccg cctccttcac gtcgaacgca aggatcgagc cgatcaggcg
     1261 aggccgcgtc acgtcggagc gcgcggcaag ctccgccatg aaggcgcgat gcgatcggct
     1321 gatacgcgcg atccgctcaa gcgttttctc ttcgccgaac agcgcaagtg aagcgagccc
     1381 gaccccgcac gcgagcggat ttgcggtaaa cgaatgtcca tgggggagcg cccgctcgaa
     1441 gctctcgccg agaaaggcct cgaacagggc ctcccgcacc acggttgcgc cgaggggcat
     1501 atagccggcg gtgagccctt tcgacaggca gacgaggtcg ggaacgacct cggcctgctc
     1561 catggcgaac agcgtgcccg tccgcccaaa ccctgttgcg acctcgtcga agataatcag
     1621 cacgcccgcc tctcgtgccg actcgacgag acgcttgagg aaactcgggc ggcagagccg
     1681 catgccgccc gcgccttgca gcagaggctc gacgatcagc gcggcgaccc cggtcctccg
     1741 gtcggtcaac gtcgccgcca aggccgacag tgcgccggcc tcgcgttcgt ccacggcatc
     1801 gtcgccctcg aacgtcgcgg gataaggcag caccgtgacc tcgcacatca gatcgcgaaa
     1861 cggcgtgaag aactgcgagc cgcgtccaac cgacatggcg ccgagcgtgt cgccatgata
     1921 gccgccgtcg aaggcgatca agccgcgccg ccgcgtctct ccgcgattga tccaatattg
     1981 ataggcgatc ttgagcgcga cctcgaccga ggtcgagccg tcgtcggaaa agaagaccct
     2041 gttcagatcc ccgggaagga tccccgccaa tgcggtggca agatcggccg cgggcgcgtg
     2101 ggtgaacccc gcgaacatca catgctcgaa ccggctcgct tgctcggcga gcgccgcgtt
     2161 cagtttcggg tgcgaatgtc catgggtgca ggtccaccac gacgacacga tgtcgaggat
     2221 ctccctgccg cccgcgtcga acagcgacgc ccccttggcc cgcgcgatga cgatcggcgc
     2281 gttgtcggtc ccatgctggg tatagggatg ccagatatgg cgctggtcgc gcgcgagcac
     2341 ttccgcggcg gtcgaggctc cttgccgtgg gatctgccga tcgtcgttca tgcggcggac
     2401 cttgcgagct tggcgaggtc gatctccggc tcgatggcca tgagcgcatc cggcgtcacc
     2461 tgctcgagcc agggaatctc ggcgatcacc tccacccgcc cgtagcgctc gatggccgcg
     2521 cgattatgcg gggtctcggg cccgttgatc accacgccgg caagagcaag cccgcgccgc
     2581 ctgatggctt cgagcgagag cagcgtgtgg ttgatggtgc cgagcgtcga gcgcgcgacg
     2641 aggatgagcg gcaggtcgag ctcgcgcgca aggtcgatca cgaaggattg gtcgttgagc
     2701 ggcaccatga gaccgcccgc gccttccgcg acaagcgggc ggttgcaagg aggcggcacg
     2761 agcttcgcca tatcgatgcc gatgccctcg cgcagcgccg cctcatgcgg ggctatgggc
     2821 tctgccagca cataggcctc gggcaggatg cggtccgggt ttgcgccgga gaggcggcgc
     2881 acggtggccg agtcggtctc gggatgggtt ccggcctgga ccggcttcca gtagcaggcg
     2941 ccgagccgtg ccacgagcca agccgcgacg accgtcttgc cgacatcggt gtcggttccg
     3001 gtgacgaaaa aaccctgcac cactcaccca gcctcaaggc gctcctcttg aggcgagact
     3061 gtatagaggc caatcggccg aagcaaaggg gagtggacgc caccgcgcct ttcgggcgac
     3121 atagcggccc catgcgccgc ctgctcgccg ttgtctcact tgccctgttc gcggctttcg
     3181 cccctcaagg gctcgcggcc aagacgctgc gcatggccta cgacgccgac cccacctcgc
     3241 tcgatccgca cgagcagctg tcgggcgcga ccctccagat gtcgcatctc ctgttcgatc
     3301 cgttgatccg tttccgccag gacctttcga tcgagccgcg gctcgccaag agcttcgaaa
     3361 agatcgacga gcgcaccacg cgttttcatt tgcgtgaagg cgtgaagttt cagtccgggc
     3421 gcacgctctc ggccgaggac gtggtgtgga ccttcaaccg gctgaaacag agcgccgact
     3481 tcaaggcgct gttcgagccc ttcagcgagg cgcgtgccgt cgacgatctc accgtcgatc
     3541 tcgtgaccaa aggcccgtat ccgctggttc tcaatctcgc cacctatatt ttccccatgg
     3601 atcgggtgtt ctattcgggg cccgatgaac gcgggcggcc caaggatgcg atcgtcaagc
     3661 acggcgcctc ctttgcctcc acgcatgcga gcggcaccgg gcccttcatc gtcaccgaac
     3721 gcgagcaggg ggtgcgcctc gaactcgagc gcttcgccga ctattgggac gagggctcgc
     3781 ccggcaatgt cgatgagatc gtcttcacgc cgatcaagga gccggcgacg cgggtggcgg
     3841 cgctgctcgc gggcgatgtc gacttcatcg cgccggtgcc gccgaccgat cttgcccgcc
     3901 tcaagcaggc gtcatgctgc accctcatca ccatgccgag cacgcgcgtt ctgaccttcg
     3961 agctcaatca ggagcgcgtg ctggcgttca aggacagccg cgtgcgtctc gccatggtcc
     4021 ttgcggtgaa cggccaaggc atcgccgaga agatcatgcg cggcctcggc acggcggcgg
     4081 gccagttgag cccgcccggt tacgccggcc acgatccgga actgacgccc cgctacgacc
     4141 tcgccaaggc gaaggctctg atgaaagagg cgggatacga ggacggattc tccgtgacga
     4201 tgatggcgcc caacaatcgc tatatcgagg acgcgcgggt cgccgaggcc gtcgccgcca
     4261 tgctcgccaa gatcaacatc agggtcgact tgcagaccat gcccaaggcg cagtactggc
     4321 aacgtttcga cgagcgcgcc gccggcatca tgatgatcgg ctggcagtcg gatacgcagg
     4381 actcggcgaa tttctacgag ttcctggtga tgacgcccga caaggacaag ggttacggcc
     4441 agtacaacgc aggcagctat tccaatccgg aaatcgaccg gctgacgctg gagacccaag
     4501 ccatgaccga cagcgacgcg cgcgccgcgg tgctgcgaaa gatcgagcgc ctcatctatg
     4561 acgacgcggc gctcatgccc ctgcactggc agcatctctc ctgggccgcg cgcaacggcg
     4621 tgaagatcga tgcggtcgtc aatgtcatcg acatgccata tctgggtgat ctcgtcatcg
     4681 agtagccgaa tgaagcggtg ttgccggcag gaggaaccac gatgttcaag aaaatcatca
     4741 tcggtcttgc ggcgctgatt ctgatcttcg tcgccatcgt cgccatgcag ccctcgtcgt
     4801 accgcatcga gcgcacggcg aagatcgcgg cgccgcaggc cgatctcttc gggcaagtaa
     4861 acgacttcca caaatgggag acgtggtcgc cctgggcaaa gctcgatccc gccgccaaaa
     4921 tcaccttcga gggtcctgac tcaggccaag gcgcggtgat gaactgggcc ggcaacgaca
     4981 aggtcggcga aggaaaattg accatcgttg agagccgccc gaacgagctc gtcaaggtca
     5041 aggtcgattt cgttgagccg ttcgagggca ccaacacgtc ggagttccaa ttcaagcccg
     5101 agggcgacca gaccgccgtc acctggatca tgtcgggcga gcacaatttc atcggcaagg
     5161 cgttctgcct ggtgatgaac ggcgagaaga tgatgggcga cgatatcgaa aagggcctgg
     5221 cccagatgaa atcggcggcc aagacggcga gcaattaacc aagcttggtc attggactaa
     5281 ccggacagag ccacgcgctc cgagcgcggc atggcggtgc gcccgccatg ctgctacgat
     5341 aggcgaactt atccgctcgc ctcgcggggg gctgggatga gctcactgat tgccggcgca
     5401 gcgacagtac cgaagccgcc catgcggact cgtgccgtca cgctcatggc aggcgtcggc
     5461 attctcaccg gcctgatctc gagcctgccg tcgccgctgc ccgacctccg cctcgtggag
     5521 ccggagttcc tcatcaacgc gaagaacgtt cccctccatg cgggcatcgc ctttggcgcg
     5581 ggcatcgcta tcatgctatg gctttgggtc acccgggatc ttggcaaatg cctgctcgcg
     5641 gtcgcgctca cgatcgtggg ctggcttgtc gcggccaaca ccgcgaatga cgtgatctcg
     5701 gtgacggtga gctcagactt gttcggtacg gccgaaggcg ccaaggcgaa ccgtgaggtg
     5761 gttgggtgcg tgctggccgg tctcattgcg ggcgcgatcg gagcgggcct caccgccttt
     5821 ggcgcgggca tcgccgcgga cccgatcagg cgcgccaaag cctggattcc catcgtcgcg
     5881 actggcgcgc tgtttggcct tctcctctat ccggccgccc attggaacgc gatcgcgctt
     5941 ctgttcgtgc cctggcaggc cgcggtcgcc ggcttgatcg cccatgctct gacccgcccc
     6001 gcggcatgag cctcctcgcg tgacgatgtc cttaatcgcc gctggcttga gcttcgccgc
     6061 gcgccgtttc gcccaggcgg cgctcgtcat cttcgcgatt ctggtcatcg cattcgccgt
     6121 gcgcgcggct ctcggcgatc ccttgcgcga gatcatgggc gaagcggtgc cagacgccga
     6181 gcgcgcggct ctccggcaaa agctcgggct tgatgccccg tggccggtgc agcttgcgcg
     6241 ctatctcggc ggcgcggttc agggcgatct cggcacctcc accatctata agcggccgac
     6301 gcttgcaatc atcctcgcca agtttccggc gagcttcgag ctcgtcctcg gcgcgagcgt
     6361 gatcgtcttt ctcttgtccc ttccggccgg catctactgc gccgtgcgcc cgcgctcgct
     6421 cggcgcgcgt ctcactctca atctcagcgt tctcggcatc tccatccccg tttttctcac
     6481 cgggattttt ctcatcaccg tcttctcggt ctggctcggc tggctgccgg cttttggccg
     6541 cggcgacacg gtccgcctcg gaccctggga aaccggcctc gtcacccgcg acgggcttgc
     6601 ccacctcatt cttccttcgc ttgcgctctc atcgatcatg ctgccgctct tcatccggct
     6661 cgtgcgcggc gccatgctcg aggagctcgg ccacgactat gtgcgcaccg cgcgggccaa
     6721 gggggcatcg ccgctccgcg tgtggctcgc gcacgcgctg cgcaacgcgc ttctgccgct
     6781 cacggcgata ggcggtctcc aggtcgggac tctcatcgcc tacacgcttc tgaccgaaac
     6841 ggtgttccag tggcccggca tgggctttct ctttctcgag gccgtcaccc gcgccgacat
     6901 cccgctgatc acgacctacc ttgtcttcgt cggcctgatc ttcgtcgtca ccaacacgct
     6961 cgtcgacatc gtgagcctca tgctcgatcc ccgcgtcagc ctggaaggga cgcgatgagc
     7021 acggaggcgg tgccgaaccg ccgatccgcg ttgcgcgttc tcgcggcgga gcatcctggc
     7081 ggcgtgatcg ccgcaatcgt gctcggcgcg atcgccctcg ccgccgccgc agcccccctg
     7141 atcgccccgc aagacccgtt cgatctcgca agtttcgacg tgctcgacgc gagcctgccc
     7201 ccgtccttcc tcgaggcaag cgatccgcgc tttctgcttg gcaccgacgc gcaggggcgt
     7261 gacctcttga gcgccatcct ttttggcacg cgcatctcgc tctccgtcgg cctgctcgcc
     7321 gtggtcatcg cggcggcggt cggcgtgccg ctgggcctcc ttgccggtta tgccgggggg
     7381 agggtcgaca cggttgtgac gcgcgcggcc gatatccagc tctcgctttc caccctgatg
     7441 atggccatca tcgcgcttgc tctgtttcgc gcgggcttcg gcggagagag cctcggccgc
     7501 ttcgccgtgc cgctccttgc cgtcgtgatc ggccttgccg agtggcccta tctcgcccgc
     7561 accgcgcgcg ccgcaaccca ggtcgaggcg gcaaaggatt atgtcagggc ggcgcgtgcg
     7621 ctcggcgcca gcacgccgcg ccttctccgt caccacattc tccccaatat cgcctcgcct
     7681 ttgatcgtcg tggcgacaac gcaaatcgcc ggcgccatca tggctgaagc ggcgctctcc
     7741 tttctcggcc tcggcatgcc ggtgaccaag ccgtcgctcg gcacgctgat ccgctcgggc
     7801 tacgatctga tgttcgcggg cgcgtggtgg gtgacggtca ttccgggact cgtgctcatc
     7861 ggcctcctcg tttcggtcaa tgtgctcggc gatgccttgc gtgatgcgct gaatccgagg
     7921 cgccggtcgg cctgaaatcg ttttgtgcac cgccgtatgc ccgcatccct tgaaaactag
     7981 gtgagcctcg aaccataaga tatagtgcct tgtcctctcg ccttgtcacc ggcgcagtcc
     8041 tggccgggat cgtcgtttaa ggagcgtcta tgaccacgtc gggtgcggcc aatccggagc
     8101 ttgtcttttc cctccgccgc gtgacggagg ccgccgcctg cgccgctttc agctggatcg
     8161 ggcgcggcga cagtctcgat gccggccggg ccgcgctcga tgccatgcgg gccgcgctcg
     8221 ccgaggtcga tatcgacgcg gtcgtcgcca tcggcgaagc cacgcgcggc gaggcgcctc
     8281 agccgcagcg cggcgagcgg ttggggcatc cgggctccgc cttcaaggcc gatgtcgccg
     8341 tcgatccggt cgaaggcacg agctatctcg tccgcggcca gaccaacgcg ctggccgtgc
     8401 tcgccgttgc cgcgcgcggc gccatgatga atccggggcc cgccttctac atggagaaat
     8461 tcgtggcgag cgctccggcg ctcggcaaga tcgatccgat ctggcccgtg gaaaagaagc
     8521 tgaccacgct tgccgacgtt cttaacaagg acgtctccga tctcaccgtc ttcgtgctgg
     8581 agaagccccg ccatcgcgaa ctcgtcgatc gcatcttggc ggcgggcgcc cgcgtcgcgc
     8641 tctatcccgc cggcgacgtc gccggcgcgc tgatggccgc catccccggc tccggcatcg
     8701 atgccctgat gggaacgggt ggcacccccg aaggcgttat gtcggcatgc gccatcaggg
     8761 cgatcggcgg cgaattcctg gcgcgcctgg atccccagct gcagaccgag acaaaagcgg
     8821 tgaaggacgc cggaatcgac actgcgaaat ggtaccaacg cgacgagatc atcacctcgg
     8881 acgacgtgtt cttctgcgcc accgggatca ccaccggtct catgctcgag ggcgtggagc
     8941 ggtccaagtc acactacaag gtgcagacca tgatgatcac gggcgcaagc ggcgagcggc
     9001 agatcttgac gagctacatg ccgatcgaac gcatggcgag cattgccgcg cgcgacgtcg
     9061 cttgacgccc ggggagacgt gcctggagtg ctaaatgtcg aagcgagtcg agcatccgat
     9121 catactcggc atcgtcggtg attcggcgtc gggcaagtcg acgctgtcag tgggcgtcgc
     9181 ccagattctg ggcgaggaac actgcacagt gatctgcacc gacgactacc atcgctacga
     9241 tcgcagcgcc cgtgccgcgc tcggcctgtc ggcgctcgac ccccgcgcca actacatcga
     9301 cgtgctggag cagcatttgc ggctcttgcg ccgcggcgag gcgatcctga agccggtcta
     9361 tgaccaccgg cacggcacgt ttgcgccgcc cgaatacgtc aagcccaagg cgttcgtcat
     9421 cgccgaaggc ctgctcggct atgccacgcg cggcatgcgc gagtgctatg acgtgaagat
     9481 ctatctcgac cccgaggagg agcttcgcgt gaagtggaag atgcaccgcg acaccaccaa
     9541 gcgcggctac accgaggagc aggttctggc gcaactcgcg cgtcgcgaga tcgacagtcc
     9601 caacttcatc caaccgcaac gcacctttgc cgacatcgtc atcaggtttc agcagccaag
     9661 cgcggatgcc aacgactccg acacgcatct cgacgtccgc catatcttgc gccccacgct
     9721 gccccatccc gatttcaccc ctctgttcga cgggtcgagc aacgccgggc tgaacctgga
     9781 gcttgcccgc gaccgcgacg gcaagccggt cgacgtgctg gagatcaacg ggaacatctc
     9841 cgacaagagg gccgagcgga tcgaggatct gctgtggaac ctgatcccgg aggcggggca
     9901 tctgcgcgat gaggtcggcc gcatcgatat cgccaatgga gccaagagcc accccttggc
     9961 gctgacccaa ctcctcgtcg ggtatcatgc ggtcaaggcg gcgatgggcg tccgcgctga
    10021 ttagcaacct ccccctcgtg ggaaggtcgg cgcgctttgg cgcgccgggt tgggggatcg
    10081 tataatcggc ggcatggcca atgagtttgc gcgcaagatg cgcaaaagca tgacccgcca
    10141 ggaattcaag ctctggcgac atctgcgcga actccgacag ctcggatttc actttcggcg
    10201 gcagactccg accaagaact tcatcgtcga ttttgcctgc tattacccaa gagtgatcgt
    10261 cgagctcgac ggcagccagc accgcaggaa cgccaatcgc taagagacaa atcgcgcgac
    10321 ctgaaactgg aggctgacgg tttcacaatc atccgcattt ggaacaatga ggtcgataca
    10381 aacctggaag gtgtcttcga tcgaatactt cacgcgctcg ggactggtcg tccccccacc
    10441 ccggcggcgc acgggcgacg ccggacctcc ccacgagggg gaggttagag aaagtcctct
    10501 gcatcagaag ttctcgccca ccgactgctg cggcggccct tggtactggc tgcacccccc
    10561 tccatgtgga tggcgtcaat tgagcgctgg cgcgccccat atggtgtgct aggagacggc
    10621 agcggagtcc tcgcgttttt taccgaaccc gttgccgcgg gcgagcgcaa agcgacacac
    10681 agcaagcctt tgatctatgg acacaattga gcctacaacg gacctgattc agggtcaggc
    10741 cttaccggag gcgtctcatc gggccatggc caacgccatc agggcgctcg ccatggacgc
    10801 ggtgcaaaag gccgattccg gccatcccgg catgccgatg ggcatggccg atgtcgccgt
    10861 cgtgctgttc acgcgcttcc tcaagttcga cgccgcgcac cccgattggc ctgaccgcga
    10921 ccgcttcgtg ctctcggccg gccacggctc gatgctgctc tacgcgctgc tttatctcac
    10981 cggttatccg aagatggagc tggaggagct caagaacttc cgccagctgc actcccgcac
    11041 cgcgggccac cccgaatatg gccatgcgcc cggcatcgaa accaccaccg gccccctggc
    11101 ccaggggctc ggcaatgccg tcggcatggc gctcgccgag cggctcctca atcgccgcta
    11161 cggcgacggg atcgtcaacc attacaccta ttgccttgcg ggcgacggct gcctcatgga
    11221 gggcttgagc gaggaggcga tatctctcgc cggccatctc aagctcggac ggctcatcgt
    11281 cctcttcgac gacaatcacg tctcgatcga cggtcccacc agcctcgccg tctccgacga
    11341 tcagatcgag cgcttcaagg cctcgcactg gcacaccgcc cgcatcgacg gtcacgatcc
    11401 cgaagcgatc gctcttgcca tcgagaacgc gaagcaggtc accgatcgcc cctcgctcat
    11461 cgcctgccgc actgtgatcg gctacggcgc gcccaacaag gcgggcacgg ccgcgaccca
    11521 cggctctccc ctcggcgaag aggaggtcaa gggcgcgcgc gagaagctcg gctggcgtca
    11581 ccagcctttc gaggtgccga agccgatcct cgcgcgctgg cgcgctctgg gctcgcgcaa
    11641 ccgcgccgcg tatgacgcct ggaacgccgc cgcaaagcgg ctcgatgcgt cagcccgccc
    11701 cggactgacc gatcccatcg acgacaaagt caaagcggcg atcgtgaccg cgatgggcgc
    11761 gatcaagtcc gacttttccc gcgaagccgc caagcttgcg acgcggcaat cctcgcagaa
    11821 agtgctggag aagcttctgc ccgtgatccc cggcctgatc ggcggctccg ccgatctcac
    11881 cggatcgaac ggaacgctca ccaagcttca caccgtggtc aagccgggcg acttctccgg
    11941 cgactacatc cactacggcg tgcgcgagca cggcatggcc gcggcgatga acggaatggc
    12001 gctgcatggc ggcatcgttc cttatggcgg cacgttcctc gtcttcaccg attactgccg
    12061 tccgtcgatc cgcctctcgg cgctgatgca gcagcgggtg atctatgtga tgacccacga
    12121 ttcgatcggg ctcggcgaag acgggcccac gcaccagccg atcgagcatt tggcggcgct
    12181 tcgcgccatg ccgaatctca atgtgatgcg ccccgccgat caggtcgaat gcgccgagtg
    12241 ctgggagctt gcgcttctcg caaagacgac gccgaccatc cttgcgttga cgcgccagag
    12301 tgttccgctg ctgcgcaccg cgtcaaccgg cgacaatctc tcggcaaagg gcgcctacgt
    12361 gctgatcgag cccagcgagc ggcgcgacgt gacgctgatc ggcacgggct cggaactcgc
    12421 cttggcggtc gaagcggcaa aggcgctcga agggaacggg atccgcgccg ccgtcgtgtc
    12481 gatgccgtgt tgggaactgt tcgaaaagca aagcgcgagt tatcgggatt ccgtcctggg
    12541 ctctgctccc cgggttgcgg tagaagccgc cggcccgttc ggctggtcga gatggctggg
    12601 gcctgacggc gccttcatcg gcatgcaggg attcggtgca tccgcgccgc agcaacacct
    12661 atataagcat ttcggaatta ccgccgaggc ggttgccgcc gcggctcgtc agctgattga
    12721 gaagaggtag agtaggggat gtcgaataag cgcgttgcca ttaacggatt tggccggatc
    12781 ggcaggctca cgttcagggc ctatttggag tccggccgca acgatctcga tttcgtcgcg
    12841 atcaacgacc tcggctcggc cgagatgaac gcccacctgc tccagtacga cagcgtgcat
    12901 gggcgctttc cgggcaggat cgatgtcgtc gacgggcacc tcaagaccaa tgggcaagat
    12961 gtggcggttc tgtcgcaagc cacgcccgaa aaacttccct ggcgcgacct tggcgtcgat
    13021 atcgtgatgg aatgcaccgg ccgattcacc gaccgggagg gcgccgccaa gcacctcacg
    13081 gccggcgcga agcgcgtgct tgtctccgcg ccgtgctccg gcgcggacct gaccgtcgtc
    13141 ttcggcgtca atcacgaggc gctgacccag gagcaccacg tcgtctccaa cgcctcgtgc
    13201 accaccaact gcctcgcccc ggtggcgaaa gtcctgaacc aggccatcgg catcaagcac
    13261 ggttacatga ccacggtgca cgcctatacc ggcgaccagc ggccggtgga cacgctgcac
    13321 aaggatccct accgggcgcg cgcgagcgcc atcaacctga tcccgagctc gacgggagcc
    13381 gcaaaggccg tcggcctcgt cctgccggag ctgaaaggca agctcgacgg cgcctcgatc
    13441 agggttccgg tgccgaatgt gagcctggtc gatctgacct tccaggcggc gcgtcccacg
    13501 actgccgagg agatcaatca ggcggtgatc aaggcggcca acggtccgct caaaggcatt
    13561 ctcggcgtga acgcgaagcc tctcgtttcg accgatttca accacgaccc attgagctcg
    13621 atcttcgacc tcaccggcac gcacgtggtc gacgacacct tctgccgcgt cgtcgcctgg
    13681 tacgacaacg aatggggctt ctccaaccgc atgtcggaca cggccgtcgc catgggacgg
    13741 ctgctgtgaa cgagacgaaa ccggccgccc agaaaccggg ggccggctca agtctcgacc
    13801 tctcccgcat caggaccatc gaggggatcg acgtgcgcgg gaaacgcgtg ctcatccgcg
    13861 tcgatttcaa cgtgccgatc gagaacggcg tcgtcgccga cgcgacgcgg ctcgagcgcg
    13921 tgcttccgac catcgcgaga ctggcgcaag ccggcgccaa ggtcgtggtg ctgtcgcatc
    13981 tcggccgtcc caaggacggt ccatcggtcg atacctcgtt gcggcccgtc gcgctgaaga
    14041 tgcgggaact gatgccggga acaaaggtgc acttcatcgg cgactgcgtc ggcgaagagc
    14101 caagacgggg cttggccgcg ctcaagccgg gcgagatcgc cgtgctcgag aatgtgcgct
    14161 tctatcccgg agaggaaaag aacgacccgc aattcgccag acgcatcgcc gagcatggcg
    14221 atctttatgt caacgacgcc ttctccaccg cgcacagggc gcatgccacc gtcgacgtca
    14281 tcgccgatct tctcccctcc catgccggcc tgacgatgat ggccgaaatt gccgccctcg
    14341 gccgcgcgct cgaaaacccg gaacgtccgg tcatgggcat tgtcggcggc gccaaggtct
    14401 cgaccaagat cgaagtcctg acccatctcg tgtcccgcat ggacgttctt gtcgtcggcg
    14461 ggggcatggc caacaccttc cttctcgcgc aagggatgaa ggtcggcgcc tcgttgtgcg
    14521 aacccgattt cgtgccgacg gcgaaagacg tcatggcgcg tgccgcgcgg agcggttgtg
    14581 agatcgtgct gccttccgac gtggtgatcg ccaatgcatt gaaggagggc gtggattggc
    14641 gggtttgccc cgtcggcgaa gtgccggatg gcgcgctcat tctcgatttt gggcctgaga
    14701 gcatcgccgc gctcgagcag cgcctgaaag gcgtccgcac cgtgctctgg aacggacctc
    14761 tcggcgcctt cgagacggcg cccttcggcg aggggacgtt tgcgctcgcc cgcgaggtgg
    14821 cacgtctcac cgaggcgggc aagctcgtca gcgtcgccgg cggcggcgac acggtgcggg
    14881 cgctcaagga ggcaggcgcc gcatcgggct ttacctatgt ctcgaccgcg ggcggcgcgt
    14941 tcctcgagtg gctcgggggc cacgaattgc cggcggtccg ggcgcttgcg aggagcgggg
    15001 cccgctccgc gatctagccc agcggccgga gattccggtt gatttctgta cttgtcattc
    15061 ccgcggatgc cggaatctag taaccatcga tcgtcggatt gtggcttgtg ccggcttgcg
    15121 gcattgcccc gtagcgcccg gcagtgacat agaggacagg gctcgaggcc atgagcgtga
    15181 tcaggagaca agggcaagga ccc
//
LOCUS       DASUQP010000254         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1079414, whole genome shotgun sequence.
ACCESSION   DASUQP010000254 DASUQP010000000
VERSION     DASUQP010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079414"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFY74_11665"
     CDS             complement(<1..920)
                     /locus_tag="VFY74_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5932385.1"
                     /translation="MALLDATEGELLTEDSGSAGARIPRQPVEAASKQYDLVIVGGGI
                     YGVALAFEAARRDYRPLLLERDDFGSGTSWSSLRIIHGGLRYLQTLDLSRFRESVAER
                     RWFLRHFPDLVRPLPCLMPLYGYGLKRPSFFRIALACNDLLSLDRNWDLEARARLARG
                     EVLSRGETLRLFPQARRHGLLGGALWWDAVMLSSERVLMELLGWACSCGATALNYVEV
                     ERILIEHGRITGVEARDRAADRVHRYAAGSVVNATGPRARAFAQRIDPAAPALFWPTL
                     AFNLLIDRAPPAEVAIAVQPRREGQIYFVLP"
     gene            complement(1158..1598)
                     /locus_tag="VFY74_11670"
     CDS             complement(1158..1598)
                     /locus_tag="VFY74_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX5932386.1"
                     /translation="MSEQLERSKIAELTTEIVAAYVSYHQIRPTDVPNIINAVGIELA
                     ALGRETEAGAPKKTEPAVSVRRSVRPDHLVCLICGKKQKLLKRHLTVEHELTPDEYRQ
                     KFGLKPDYPMVAPDYAQQRRELALSIGLGKPKKPAKRRRKSAAR"
     gene            complement(2219..>2800)
                     /locus_tag="VFY74_11675"
     CDS             complement(2219..>2800)
                     /locus_tag="VFY74_11675"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5932387.1"
                     /translation="AFSWDWWAAGTAQAGLITDGEWWRTLTALGLHADVGHLASNLFF
                     GSVLGLLLAQALGSGFAWLAILLAGGLGNAMGALLQPATHAAVGASTAVFGALGILAA
                     LAWKRQAAVWRLRLRRWLPFAAGLMLFAYLGVGGDRTDVVGHIAGLAAGGGIGVALGA
                     RKSPKGRRVQWACGTAAAALFALAWALALRAHG"
BASE COUNT          523 a          922 c          889 g          466 t
ORIGIN      
        1 ggcagaacga aatagatctg cccctcgcgg cggggctgga cggcaatggc tacctcggcc
       61 ggcggcgctc gatcgatcag caggttgaac gccagcgtgg gccaaaagag cgcgggggcg
      121 gcaggatcga tgcgctgcgc aaaagcccgc gcgcgcggcc ccgtggcgtt gaccacgctg
      181 cccgccgcat agcggtggac ccggtcggca gcgcggtccc gcgcttcgac gcccgtgatc
      241 ctgccatgct cgatgaggat gcgctcgacc tccacgtaat tgagcgccgt cgcgccgcag
      301 ctgcaggccc agcccagaag ttccatgagc acccgctcgg agctgagcat gaccgcatcc
      361 caccagagcg caccaccgag aaggccgtgg cgccgggcct gtgggaagag cctaagcgtt
      421 tccccacggc tgagaacctc gccgcgcgcg agccgggcac gcgcctcgag gtcccagttg
      481 cggtcgagcg agagcagatc attgcaggcg agcgcgatcc gaaagaagct tggacgcttg
      541 agaccgtagc catagagcgg catcaggcag ggcagcggcc ggaccaggtc cggaaagtga
      601 cgcaggaacc agcgtcgctc ggccaccgat tcgcgaaagc ggctcaggtc caaagtctgt
      661 aagtagcgga gccctccgtg aatgatgcgc agactggacc agctggtgcc gctcccgaag
      721 tcatcacgct cgagcaacag cggacgataa tcgcgccgcg ccgcttcgaa agcgagcgcg
      781 acgccataga tcccgccgcc gacgatgacg agatcgtact gtttcgatgc ggcttccaca
      841 ggctggcgag gaatgcgcgc gccggcgctg ccgctgtcct ctgtaaggag ctctccctcc
      901 gtggcgtcga gtaaggccaa gcgcagcgct ccttggcggc ggtcaaggcg cgatcgtcgg
      961 ccacatctcc cgatcagcca gcctccaatg gccgacccga aaagggccgc tggcgatctc
     1021 ctcgcgatat cgatcctagt ctggccgacg tcgcgatgct tggcgagtgc gacctcggcg
     1081 ccgccccgag gcaagggggg ccgcgcgcgc tcgatgcccg ccccggggcg atctcagctt
     1141 cgagcgcgca gaccggttca gcgggcggct gacttgcgcc tgcgcttggc gggcttcttt
     1201 ggcttgccga gcccgatgct caacgccagt tcgcgccgtt gttgcgcata atcgggagcc
     1261 accatcggat agtcgggctt caagccgaac ttctgccggt actcgtcggg cgtcagttca
     1321 tgctcgaccg tgaggtgccg tttcaacagc ttctgcttct taccgcagat caggcagacg
     1381 aggtggtctg gtcgaactga tcggcgcacg gagacggcgg gctccgtttt cttcggcgct
     1441 ccggcttcgg tctctcttcc aagcgccgcc agttcgatcc cgactgcgtt gattatgttt
     1501 ggaacgtcgg tcgggcggat ctggtggtag gaaacgtatg ctgcgacgat ctcggtcgtc
     1561 agctcggcga tcttcgatcg ctccaactgc tcgctcattt tcaactcccc tcgtcaccta
     1621 accttgccgt gacataaact gtccagcttt gtttggcaat tattagatac gtgacgatgg
     1681 atttcgaacc aacgcgttcc cggaaagtgt cgtttggtga cggtcagata cgagaggaac
     1741 accgtcgtca aagcaccggt tctcgcgttc cttgtcgtac ggcttttcgg ggcgcgagac
     1801 cgtttcctac tcatccagcc ggcccacttc tacagtcttt aggcagggag atgcgctacc
     1861 tgaaattcaa tccgaccgcg cgtttttcat gtgttcgcac gcgctttgat gtcaccgccc
     1921 tcgcagaaca caacaagccg gtgtggcagt catagtagcc ctgggcgcgg gtcctacgca
     1981 aagaaacgcg cgacccttag ttcgtagcac catctcacat cgccaatcgt cgaagcaaca
     2041 ccaggaggcg tcggtgaacg gtgccttatc ggctcaatgc tggatctcag gtcccaggca
     2101 tttcgcgctc aggtactgct gcgttcggta cgccgcgatc aatgatgaaa gaatcagccg
     2161 cacgcttgct gggccacctc atgccgtcac tgaggcgaga gcgggcggaa cgagggactt
     2221 atccatgggc ccggagcgca agcgcccatg cgagcgcgaa cagcgccgca gcggccgtgc
     2281 cgcaggccca ctggacgcgc cgtcccttgg gagacttacg cgcgcctagc gcaacgccga
     2341 tcccgccgcc ggccgcaagg ccggcaatat ggccaacgac gtcggtccga tcaccgccga
     2401 cgcccagata ggcgaagagc atcaagcccg ccgcaaaggg cagccatcga cgaagccgaa
     2461 ggcgccagac ggcggcctga cgcttccagg cgagcgccgc gagaataccc agcgctccga
     2521 acaccgccgt ggaggcgccc acggcggcgt gggtcgccgg ctgcagcagc gcgcccatgg
     2581 cattgccaag gcctccggca agcagaatgg ccagccaggc aaagccgctg ccgagcgcct
     2641 gggcgagaag aagaccgagc acgctgccga agaagagatt gcttgcaagg tgaccgacat
     2701 cggcgtgcag cccgagggcg gtcagggtcc gccaccattc gccgtccgtg atcaggcccg
     2761 cctgcgcggt ccccgctgcc caccagtccc atgagaaagc
//
LOCUS       DASUQP010000255         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2034235, whole genome shotgun sequence.
ACCESSION   DASUQP010000255 DASUQP010000000
VERSION     DASUQP010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034235"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFY74_11680"
     CDS             complement(<1..667)
                     /locus_tag="VFY74_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX5932388.1"
                     /translation="MSTKVSGPQGPVISSRDELVAYLEQGSKPAHEWRIGTEHEKFGF
                     YRKGHDPVPYDGERGIRALLEALGDRFGWDQVKEHGNIIALRRHDCPKGGAVSLEPGG
                     QLELSGAPLENLHETYEELRQHLFEVGEAGGQLGIGFLGLGFSPKWTLAETPMMPKER
                     YRIMARYMPKRGRRGLDMMFRTATVQVNMDFGDEADMVAKMRAGLALQPVATALFANS
                     PFTE"
     gene            complement(759..1490)
                     /locus_tag="VFY74_11685"
     CDS             complement(759..1490)
                     /locus_tag="VFY74_11685"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX5932389.1"
                     /translation="MSPKLQRLFVTSKLGAGATVTLRKDQAHYLGNVLRLKQGDNLLL
                     FNGKDGEWWAELGAIGKKEAQARLDHQTRPQEDGPDLHYLFAPLKRARLDYMVQKATE
                     LGASALRPVITRHTVTERVKTGRLLANAIEAAEQCGILRVPEIMEPEKLTKLLDHWDE
                     GRLLIFADESAPHASPIEALKTRKPGPLAVLIGPEGGFEREERAALLTKPFVLPISLG
                     PRVMRADTAAVAALALVNAALGDWQ"
     gene            1536..2489
                     /gene="ubiA"
                     /locus_tag="VFY74_11690"
     CDS             1536..2489
                     /gene="ubiA"
                     /locus_tag="VFY74_11690"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX5932390.1"
                     /translation="MTTSLPQDKASAIADAGARNWVDRFAPKPLIPYLRLARADRPVG
                     FFLLAFPCFWSVSLASRSLDEAYPDPRLLALFALGALVMRAAGCTYNDIVDRDIDAQV
                     ARTRARPIPSGQVGVRSATFFMVALSLIGFVVLLSFNSFTIWLGLGVLPIVALYPFVK
                     RFSSWPQAVLGLAFNWGALLGWTAVLGRLDWPAVVLYVGAVAWTIGYDTIYAHQDRDE
                     DGVLGLKSTALRFGRSTRAWLAALYALAFLAITAAGLMANAGTAFLLGMVAAGAHLAW
                     QVATLNIDDPENCLERFRSNRDFGALVLGAILVDAALAAIF"
BASE COUNT          410 a          860 c          851 g          538 t
ORIGIN      
        1 cctcggtgaa cggcgaattg gcgaacaatg cggtcgccac cggctggagc gcgaggcctg
       61 cgcgcatctt ggcgaccatg tcggcctcgt cgccgaaatc catgttcacc tgcaccgtcg
      121 cggttcggaa catcatgtcg agcccgcggc gtccgcgctt cggcatatag cgcgccatga
      181 tgcggtagcg ctccttcggc atcatcggcg tctctgcaag cgtccatttc ggagagaaac
      241 ccaagccgag aaagccgatg ccgagttgcc cgccagcctc gccgacctcg aagagatgct
      301 ggcgcagctc ttcgtatgtt tcgtgcagat tttccagcgg cgcgcccgag agctcgagct
      361 ggccgccagg ctcgagactg accgcgccgc ctttcgggca atcatggcgg cggagcgcaa
      421 tgatgttgcc gtgctctttg acctgatccc agccgaagcg atctccgagc gcctcgagca
      481 gcgcgcgaat gccgcgttcg ccgtcatagg ggaccgggtc atgccccttc cggtaaaagc
      541 cgaacttctc atgctcggtg ccgatgcgcc attcatgcgc cggctttgag ccctgctcga
      601 gataagcgac gagctcgtcc cgtgaagaga tgacgggccc ttgaggccca ctcacctttg
      661 tcgacatgaa ggggagctcc gtgcagggtt ggggcgcggg tgggaaccgc gaagcgcggc
      721 ctttatggcg ctctccctat gcgcctgaca agcggtcctt attgccagtc gccaagtgct
      781 gcgttcacaa gggcgagagc ggcgacggcc gccgtgtcgg cccgcatgac ccgcggcccg
      841 agcgaaatcg gaaggacgaa gggcttcgtc agcagtgccg cccgctcctc gcgctcgaac
      901 ccgccctcag gcccgatcag gacggcaaga ggcccgggct ttcgggtctt gagtgcctcg
      961 atgggcgaag catgaggggc cgactcgtcg gcgaagatga gaagcctccc ctcgtcccag
     1021 tgatcgagga gcttggtcag cttctcgggc tccatgatct ccggcacgcg caagataccg
     1081 cattgctcgg ccgcctcgat ggcgtttgca agcagccgtc cggtcttgac ccgttcggtc
     1141 acggtgtggc gcgtgatgac cgggcggagc gcgcttgcgc caagctctgt tgccttctgc
     1201 accatatagt cgaggcgggc gcgcttcagc ggcgcgaaaa gataatgcag gtcggggccg
     1261 tcttcctgcg gccgggtttg gtgatcgagg cgggcttgcg cttccttctt gccaatggcc
     1321 ccaagctcgg cccaccactc cccgtccttg ccgttgaaca gaaggaggtt gtcgccttgc
     1381 ttgagtctca acacatttcc gaggtaatgc gcctggtcct tcctcagcgt gacggtagcg
     1441 cccgctccta gcttggatgt gacgaacagg cgctggagtt tcggagacat ggcggtttcg
     1501 cgagtgaagg acggcggagc ataggcgtca agcacatgac aacgtccctg ccccaagata
     1561 aggcgagcgc gattgcggac gcgggcgcgc gcaattgggt ggaccgcttc gcgcccaagc
     1621 ccctcattcc ctatctcagg ctcgcccgcg ccgatcgccc ggtcggcttt ttcctgctcg
     1681 ccttcccctg cttctggtcg gtgtcgctcg cgtcgcgcag cctggacgaa gcctatcccg
     1741 atccgaggct ccttgccctg ttcgcgctgg gcgcgctcgt catgcgcgcc gcgggctgca
     1801 cctataacga catcgtcgat cgcgacatcg acgcgcaggt ggcgcgcacg cgggcgcggc
     1861 cgatcccgag cggacaggtc ggcgtcagaa gcgccacctt ctttatggtt gcgctcagcc
     1921 tgatcggctt tgtcgtgttg ttgagcttca acagcttcac catctggctc ggactcggcg
     1981 tcttgccgat cgtggcgctc tatcccttcg tcaagcgatt cagctcctgg ccgcaagccg
     2041 tgcttgggct cgccttcaat tggggagcct tgttgggctg gactgccgtt ttgggccggc
     2101 tcgattggcc ggccgtcgtg ctctatgtgg gagccgtggc ctggaccatc ggctatgaca
     2161 cgatctacgc ccatcaggac cgtgacgaag acggcgttct tgggctgaaa tcgacggcgc
     2221 ttcgttttgg ccgatccacc cgcgcctggc tcgcggccct ctacgcgctc gcctttctcg
     2281 ccataaccgc ggcggggctc atggccaatg ccgggacggc gtttctcttg ggcatggttg
     2341 cggccggggc gcatcttgcc tggcaggtcg cgactctcaa catcgacgat ccggagaatt
     2401 gtcttgagcg ctttcgctca aaccgcgact tcggcgcgct cgtgctcggc gccatccttg
     2461 tcgatgcggc gcttgcggca atattctgag ttgcgatgag ccgggcgaaa gcgcaaaaaa
     2521 gcaccgggcc ccggggctta tcgcccaggg gcccggatga cgccttgctg ttagcttccc
     2581 cgttcggctg gctgccttga ggcggccttg attggcgcga tcctgccatg gaagttttcc
     2641 gaagcgctta acggacgtt
//
LOCUS       DASUQP010000256        10172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1352466, whole genome shotgun sequence.
ACCESSION   DASUQP010000256 DASUQP010000000
VERSION     DASUQP010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352466"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFY74_11695"
     CDS             <1..563
                     /locus_tag="VFY74_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5932391.1"
                     /translation="LGGGLGIPYRSDQPPPPLPAEYADLVKEEVGDLGVRLLLEPGRM
                     IAGNAGILVSRVLYVKRGASKTFTIVDTAMNDLLRPTLYDAHHEILPVVEPKPGAPLV
                     VTDIAGPVCETGDYLALARTLPELQAGDLISVMTAGAYGAVLASEYNSRLLVPEVLVA
                     GNRYGVTRPRPSYGDMLAKEQLPEWL"
     gene            655..3357
                     /locus_tag="VFY74_11700"
     CDS             655..3357
                     /locus_tag="VFY74_11700"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEX5932392.1"
                     /translation="MRSASRPKTPSPEPVEHGALAQRFDRRVRLSWLALLGERVWEAL
                     LWPFLVVLTFLVVSLLELWSLLPPLAHRILLCGFGLALIVSFLPLLRILLPTRQEALR
                     RLERHAGIKHRPASSYEDSLGAPSPKETAALWAAHRERLSRLIAKLKPSWPAPRTDRK
                     DPYAVRAALLLGVVAAALAAGGNVSDRLRAAFAPAATSAPAMLRLDAWVTPPVYTGIA
                     PIVLADGSEKVGAGAESFRALSVPERSELIVRTHAPQGETVSLITSRDGATEATKVAP
                     KKGGAEGLVEFNVALNAPLSAAVEIGGQTVSKWRFDLIEDTVPTINLMGTPTKAPRGA
                     LRFVFRADDDNGVASAEARFALGEGEERNLAPIPSEASPKVEADPLLEPPVMPLQLPR
                     ANAKRVDGRATQDLTAHPWAGLKVRMTLMARDQAGQSGESVPYEFVLPERTFTKPLAR
                     AVVEQRKKLVRDPGAPEGVANALDALTLGGDKVIDDSVVYLALRNAYWRLRGDRSAEG
                     IASVVDQLWSTALRIEEGDLPEAERALSAAQDALMQALKEERSPEEIKNLVDELRSAL
                     SRYLQALAAQQQEKGETSPLAQQDGDQLVSQQDLEKILSNIQQLAQSGSKDLAERMLS
                     ELKDLLERLETGTMAETEQQQRARRMMKDLSDLVSNQQKLLDDTFAAKRQQAARSGGD
                     SQQFEVSPPGQPMEFGPGMAPLFEELPGEAQQGFMKGQGESGGQGGPREGGQLEISPD
                     AARSGEHGKLAERQQELLDRLQTLIDRFRIEGSDAPTDLQDAGEAMGDAKEAIGESDL
                     ERATQQQSLALDRLRQGAQSMAEQMMEGAETQAGQGPGNNGRDPLGRPDRSNRPDLGL
                     SVQVPDEIDIQRAREVLDELRRRLGDPSRPALELDYLERLITPF"
     gene            complement(3387..3935)
                     /gene="hpt"
                     /locus_tag="VFY74_11705"
     CDS             complement(3387..3935)
                     /gene="hpt"
                     /locus_tag="VFY74_11705"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5932393.1"
                     /translation="MAEEPAAAQTIRTLFSAEEIAGRVESLARAIAASAPRDFVIVAI
                     LKGSFIFAADLIRALHREGLRPEIDFIFLASYGAGTVSGGAVRILRDVEVALAGREVV
                     IVDDILDSGQTLHFAKTLLEERGPKSVRTCVLIDKHVPRAAAIVPDFSGFRCPPVFVV
                     GYGMDLAHRHRELPFIGEVAKP"
     gene            complement(3938..4792)
                     /locus_tag="VFY74_11710"
     CDS             complement(3938..4792)
                     /locus_tag="VFY74_11710"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEX5932394.1"
                     /translation="MGGFAGIAPAAPAPPRFPPPPVSEAELAAQVARINAEAMAEEPP
                     AAQPEKRGGIFSRLTRKREPEPPAPPPAQAGLGDAFKPDAGFAGDASMDEGPTADAGI
                     GAGLDAAMSDAGMSDAGMSDAGMGIDAGLAEEALPSEQPPRKGSRVTMGWLLLLLVVA
                     GVIGTLALAPSTVLSVLPGASRLYALFGMPVGARGLALESVRYGWTSDGGLEVQGDVV
                     NLTSSPVAAPTVVIALRDQGGEEISEWTTEIGEEQLAGGQRAPFLRQIPSPPSNVRSV
                     AVRFAKAK"
     gene            5180..5836
                     /gene="ftsE"
                     /locus_tag="VFY74_11715"
     CDS             5180..5836
                     /gene="ftsE"
                     /locus_tag="VFY74_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX5932395.1"
                     /translation="MIRFDNVGLRYGTGPEILRDVTFHVHPGSFHFLTGPSGAGKTTL
                     LRLLFMSLKPSRGLIFVFGENVAELSPAGRSALRRRIGIVFQDFRLLDHLTTWENVAL
                     PLRVMGKKEGDYREDVTDLLRWVGLGDRINAYPEVLSGGEKQRAAIARAVIARPELLL
                     ADEPTGNVDPAIGNRLLRLFVELNRLGTSVIIATHDYGLMEQLDAPRLVLYDGHLQIR
                     "
     gene            5843..6841
                     /locus_tag="VFY74_11720"
     CDS             5843..6841
                     /locus_tag="VFY74_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5932396.1"
                     /translation="MTLLSDIKDYPWKSLLAGLLPARLAALLPQRFQTKSAQIVPPAS
                     VAGNALTVVIAIMSFLACLTLGAVYMINQSANAWVNDIASEITVELDPVNAADIEKKM
                     TLVSLFLAKQKGITNVKPLSAEDSARLLEPWLGHSSALQALPIPRLIAVEIDRSNPPD
                     IQLIKTALSENFEGVVLDDHRRWQAEIRTLTRSAALGGLAVLALVAAATIAVIVSATR
                     SAMASNREIIEVLHFVGANERFISREFERHFLGLGVRAGLMGAAAAALAFLLLPAMID
                     MLGGGIVTTAETQRLIGSGALDFKGYLLCVLVVIVVASLCMITSRLGVIRVLKNYA"
     gene            6900..7622
                     /locus_tag="VFY74_11725"
     CDS             6900..7622
                     /locus_tag="VFY74_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5932397.1"
                     /translation="MPILPPNWHVLESAVRAEQEERRGRFSGFLGGVRLIFDGVLFLF
                     VLFIIGFIVFANGIDRKQSEPQETADGIAVLTGGTSRIDRAMKLLAAGKAKRVLITGV
                     NRTTTVEELKQLASQGGQYFTCCVDIDKEARNTIDNATETSEWVALHRYRSVIVVTSN
                     YHMPRALAELARVMPGVTFIPYSVVDNNVHLDRWWTYPGTTKLLLSEYLKYLPALGRL
                     GATELVRRFLGGDPLTGDVPEP"
     gene            7637..8380
                     /locus_tag="VFY74_11730"
     CDS             7637..8380
                     /locus_tag="VFY74_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5932398.1"
                     /translation="MARSLLFNLLFYATTVLFLVLGSPLLFGPRRWAMAALAIHGRFE
                     LWLLERIVGTTLEVRGLDKVPDGACLVAAKHQSAWETFGLIPLFRDPALLMKRELFWI
                     PLHGWFSHKFEMIPVDRDKGPAALRRMVREAKKRAAQGREIIIFPEGTRRAPGAPPDY
                     KTGVVLLYEALGIPCVPLALNSGAFWPRRSFVRHPGIIVVEILDPIPPGLPKAEFLPR
                     LIATIETASARLLAEAKAEEHGVGRPVNL"
     gene            8539..8916
                     /locus_tag="VFY74_11735"
     CDS             8539..8916
                     /locus_tag="VFY74_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932399.1"
                     /translation="MGLADEPALVCSEAAFAQAFHYWRGASGRRYLHSVYSLIGCPAL
                     PRANYILVRRYDDGTRAALAFGQTKDETASLNLAHLRHQGAKCGANEVHIHLLADTPD
                     ARAEVEADLLAAHTRRLATANAA"
     gene            9042..9617
                     /locus_tag="VFY74_11740"
     CDS             9042..9617
                     /locus_tag="VFY74_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932400.1"
                     /translation="MAGLFKNPWLRRQRQRLRRPLQVLASPALANYINLVLNTSNQIA
                     DPPDFIARAKAQHPQIIAMWHGQFLLLPGIYPPEIPGRAMIARHDDAEALARVTAGAD
                     MARATPEKRRPAHRCSQNAATGRAYAPAGTGTCFHPCADGPSHNHRSRRRHLTPPTYS
                     GNLSKSCCWRCRLRVSASVSQVSAISINERL"
BASE COUNT         1753 a         3473 c         3183 g         1763 t
ORIGIN      
        1 atctcggcgg cgggctcggc attccctatc gaagcgatca gccgccaccg ccccttcccg
       61 ccgaatatgc cgatctcgtc aaggaggaag tgggcgatct cggcgtgagg ctcctcctgg
      121 agcccggccg catgatcgcg ggcaatgccg gcatcctcgt ctcccgcgtg ctctatgtga
      181 agcgtggcgc atcgaagact ttcaccatcg tcgatacggc gatgaacgac ctcttgcggc
      241 cgacgctcta cgatgctcat cacgagatcc tgccggtcgt ggagccgaag cccggcgcgc
      301 ccctcgtcgt gaccgatatc gccgggccgg tctgcgagac gggcgactat ctcgcgcttg
      361 cgcggaccct gccggaactg caggccggcg atctcatttc cgtgatgacg gccggcgcct
      421 atggcgcggt gctcgcctcg gagtataaca gcaggcttct ggtccccgag gtgctggtgg
      481 caggaaaccg ctacggcgtg acgcggccga ggccaagcta cggcgatatg ctggccaagg
      541 agcagctgcc ggagtggttg tgagcctccg gaacaggcga tgagcctcgc tttcgcccgc
      601 ttcgctcacc agattaaact tatcgcccgt ttggccccga gctcgatgaa gatcatgcgt
      661 tccgcctctc gacccaagac gccctccccc gaaccggtgg agcacggcgc actcgcgcag
      721 cgcttcgaca ggcgcgtgcg attgagctgg ctcgcccttc tcggcgagcg ggtctgggag
      781 gcgttgctgt ggcccttcct cgtcgtcctc acctttctcg tggtgtcgct gctcgagctg
      841 tggagcctgc tcccgcctct ggcgcatcgc atcctcctgt gcggtttcgg attggccctg
      901 atcgtctcct tcctgccact gctcaggata ttgctaccga cgcgccagga ggcgctgcgg
      961 cgtctcgagc gccatgccgg gatcaagcac cggccggcct cctcctatga ggacagtctc
     1021 ggcgccccgt cgcccaagga gacggcggcg ctgtgggcgg cgcaccgcga gcgcctgtct
     1081 cgtctgatcg cgaagctcaa gccgtcctgg cccgcgccgc gcaccgaccg caaggaccct
     1141 tacgctgtcc gggcggcgct gctgcttggc gtggtcgcgg ccgcgcttgc cgcgggcggc
     1201 aatgtttccg atcgcctgcg cgccgcgttt gcgccggccg ctaccagcgc acccgcgatg
     1261 ctcaggctcg acgcctgggt cacgccgccc gtctataccg gcatcgcgcc gatcgtgctc
     1321 gccgacggca gcgaaaaggt tggagcgggt gccgagtcct ttcgcgccct ctcggtccct
     1381 gagcgcagcg aactgatcgt gcgcactcac gcgccgcagg gcgagacggt aagtctcatc
     1441 acgagccgcg acggcgccac cgaagccacg aaagtggccc cgaagaaagg cggcgccgaa
     1501 gggctcgtcg aattcaacgt cgcgctcaac gctcctctca gcgcagcggt cgagatcggc
     1561 ggccagaccg tttccaaatg gcggttcgat ctgatcgagg acacggtccc caccatcaat
     1621 ctcatgggca cgccgacgaa ggcgccgcgg ggtgccttgc gtttcgtgtt ccgcgccgac
     1681 gacgacaacg gcgtggcaag cgccgaagct cgcttcgcgc tcggcgaagg cgaggagcgc
     1741 aatctcgcgc cgatcccttc cgaagcctcc cccaaggtcg aggccgatcc cctgctcgag
     1801 ccgccggtca tgccgctgca attgccacgc gccaacgcca aacgcgtcga cggtcgtgcc
     1861 acccaggatc tcaccgccca cccttgggcg ggactgaagg tgcgcatgac gctgatggcc
     1921 cgcgaccagg ccgggcaatc gggcgagagc gtgccctatg agttcgttct gcccgagcgg
     1981 acgttcacca agcctctcgc gcgggccgtg gtcgagcagc ggaagaaact cgtgcgcgat
     2041 cccggcgctc cggaaggcgt ggcaaatgcg ctcgatgcgc tcacgctcgg cggcgacaag
     2101 gtgatcgacg acagcgtggt ctatctcgcc ttgcgcaacg cctattggcg gctgcgcggc
     2161 gaccgctcgg ccgaggggat cgcaagcgtc gtcgaccagc tctggtccac ggcgctccgc
     2221 atcgaggagg gcgatctgcc agaggccgag cgggccttga gtgccgcgca agacgcgctc
     2281 atgcaggctc tgaaggagga gaggtcgccg gaggagatca agaatctcgt cgatgagctg
     2341 cgctcggcct tgtcacgcta tctccaagcg ctcgccgccc agcagcagga gaaaggcgag
     2401 acatcgccgc ttgcccagca ggacggcgac cagctcgtgt cacaacagga cctcgagaag
     2461 atcctcagca acatccagca gctggcgcag tcgggatcga aggatctcgc ggagcgcatg
     2521 ttgtctgagc tgaaggacct gctggagagg ctcgaaaccg gcacgatggc cgagaccgag
     2581 cagcagcagc gcgcaagacg catgatgaag gacttgagcg atctcgtttc gaaccagcag
     2641 aagctgctcg acgatacctt cgccgccaag cgccagcagg ccgcgcgaag cggcggcgac
     2701 agccagcagt tcgaagtgag ccctcccggc cagccgatgg agttcggccc gggcatggcg
     2761 cccttgtttg aggagctgcc cggcgaggct caacaaggct tcatgaaagg acagggcgaa
     2821 tcgggcggtc agggtggccc gcgcgagggg ggccagctcg agatcagtcc cgacgccgcg
     2881 cgctcgggcg agcacggcaa gcttgccgag cgccaacagg agcttctcga caggctgcaa
     2941 actttgatcg accgcttccg catcgagggg agcgacgcgc cgaccgacct tcaggatgca
     3001 ggcgaggcca tgggcgacgc caaggaggcg atcggcgaga gcgacctgga gcgcgccacg
     3061 caacagcaga gcctcgcgct cgatcgcctg cgccagggcg cccagtccat ggccgaacag
     3121 atgatggagg gcgccgaaac gcaagcggga cagggccccg gcaataacgg ccgcgatccg
     3181 ctcgggcggc ccgaccgctc caaccgaccc gatctcggcc tgtcggtgca ggttcccgac
     3241 gagatcgaca ttcagcgcgc acgcgaagtg ctcgacgagc ttcgtcgccg cctcggcgat
     3301 ccatcgcggc cggcgctcga gctcgattac ctggagcgcc tgatcacgcc gttctgatcg
     3361 cagccgtcac cgaatggatt gggctatcac ggcttcgcca cctccccgat aaagggcagc
     3421 tcgcgatggc gatgggcgag gtccatgccg tagcccacca cgaaaaccgg cggacagcgg
     3481 aaccccgaga aatctggcac gatcgccgca gcgcgcggca cgtgcttgtc gatcaggacg
     3541 catgtcctga ccgacttggg gccgcgttcc tcgagcagcg tcttggcgaa gtgaagcgtc
     3601 tgcccagagt cgagaatatc gtcgacgatc acgacctcgc gcccggcaag agcaacctcg
     3661 acgtcgcgca ggatgcggac cgcgccgcct gaaaccgttc ccgcgccata actggcaagg
     3721 aagataaagt cgatctcggg cctcagcccc tcacgatgca acgccctgat gaggtccgcg
     3781 gcgaagatga aactgccctt gaggatggcg acaatgacga aatcgcgcgg ggcggaggcc
     3841 gcgatcgcgc gggccaagct ctcgactctt cccgcgatct cttcggcgct gaacagcgtg
     3901 cggatggttt gagccgccgc cggctcctcg gccattccta cttggccttg gcgaaacgca
     3961 ccgcgacgct ccggacattg ctcgggggcg agggaatctg ccgcaggaac ggcgcacgct
     4021 ggccgcccgc cagctgctcc tcgccgatct cggtggtcca ttcggaaatc tcctcgccgc
     4081 cctgatcgcg gagcgcgatg accacggtcg gcgcggcgac cgggctcgag gtgaggttca
     4141 cgacgtcgcc ctgaacttcg agcccgccat cgctggtcca gccataacgc acgctctcca
     4201 aggcgagccc gcgggcgccg accggcatgc cgaagagagc gtagagacgg gacgcgccgg
     4261 gcaggaccga caggaccgtt gagggcgcga gcgcgagcgt gccgatgaca cccgccacga
     4321 cgagcaggag caagagccag cccatggtga cgcgcgagcc tttgcgtggc ggttgctcgc
     4381 tgggcagcgc ctcttcggca agcccggcat cgatacccat ccccgcatcg ctcatgccgg
     4441 catcgctcat gcccgcatcg ctcatggcgg cgtccagccc ggcgccgatg ccggcgtcgg
     4501 ccgtcggccc ttcatccatg ctggcgtcac cggcgaaacc ggcatcgggc ttgaaggcat
     4561 cgcccaagcc ggcttgagcc ggcggcggag ccggaggctc gggctcgcgc ttgcgcgtga
     4621 gccgcgaaaa gattcccccg cgcttctctg gctgtgcggc cggcggctcc tcggccatcg
     4681 cttcggcgtt gatccgcgcc acttgggcgg caagctcggc ctcgctcacc ggcggcggcg
     4741 ggaaacgcgg cggagcgggc gctgccggcg caattcccgc gaaccctccc atccccgcat
     4801 tgaccgcagc gggccgcggc gggggcggag gggcctgcgg cggcatcgga gccgaagcgg
     4861 ggggtaccgg cggcctcaag ccaggcgccg aatcaggagc gcccacggcc ggggccgcct
     4921 ggacagggaa cgcctgccag acatgcgcgc acttggaaca acggactttc cggccctcag
     4981 gcggaaacac cgccttggtc tcgtaacgcg tcccgcaagc cggacagata atgagtgttg
     5041 ccatctgaaa agccaaaatg gccttttatc ctggactttg ccccgggcgc catacgccgc
     5101 ccaaacccat tgatacccaa ccgggagttc agcataaagt gccagccttc cgcaacttta
     5161 agagccttag agagcacggg tgattcgctt tgacaatgtc gggctgcgct acggcacagg
     5221 cccggaaatc ttgcgcgacg tcaccttcca tgtgcatccg gggtcctttc actttctgac
     5281 ggggccttcg ggcgccggaa agaccacgct cttaaggctc ctgttcatgt cgctcaagcc
     5341 ctcgcgaggg ctgatcttcg tgttcgggga aaatgtggca gagctctccc cggccggacg
     5401 gtcggcgctc cgccggcgca tcggcatcgt gttccaggat ttccgcctgc tcgatcacct
     5461 caccacgtgg gaaaacgtgg cgctcccctt gcgcgtgatg ggcaagaagg agggcgacta
     5521 ccgcgaggac gtgaccgacc tgttgcgttg ggtcggcctc ggcgaccgca tcaatgccta
     5581 tcccgaagtc ctctccggcg gcgagaagca acgcgcggcg atcgcgcgcg ccgtgatcgc
     5641 gcgccccgag ctcctgctcg ccgacgaacc cacgggcaat gtcgatccgg caatcggcaa
     5701 tcgcctcttg cgtctgttcg tcgagctcaa tcggctcggc acctcggtga tcatcgccac
     5761 tcacgattac ggcttaatgg agcaactcga cgccccacgg ctcgtgctct atgacggcca
     5821 cttgcagatc cgctagacgc ccgtgaccct gctctcggac atcaaagact atccgtggaa
     5881 aagccttctc gccgggctct tgccggcgcg gcttgccgcc ttgcttccgc aacggtttca
     5941 gaccaagagc gcgcagatcg tgccgcccgc ttcggtcgcc ggcaatgcgc tcaccgtcgt
     6001 gatcgcgatc atgtcctttc tcgcctgcct gaccttgggc gcggtgtaca tgatcaatca
     6061 atcggccaat gcctgggtca acgacatcgc aagcgaaatc accgtcgagc tcgatccggt
     6121 caacgccgcc gacatcgaga agaagatgac gctggtttcc ctcttcctcg ccaagcagaa
     6181 gggcatcacc aacgtcaagc cgctctccgc cgaggacagc gcaaggttgc tcgagccatg
     6241 gctcgggcat agcagcgcgc tccaagcgct ccccattccg cgtctgatcg cggtggagat
     6301 cgaccgctcc aatccgcccg acattcagct catcaagacg gcgctgtcgg agaacttcga
     6361 aggcgtcgtg ctcgacgacc accgccgctg gcaggctgag atccgcacgc tgacgcgctc
     6421 cgcggcactc ggcgggctcg ccgtgcttgc ccttgtcgcc gccgcgacca tcgccgtcat
     6481 cgtgtcggcg acgcgaagcg ccatggcctc gaaccgcgag atcatcgagg tccttcactt
     6541 tgtcggcgcc aatgaacggt tcatcagccg cgagttcgag cgccatttcc ttgggcttgg
     6601 cgtccgcgcc ggtttgatgg gagcggccgc ggccgcgctc gcctttctgc tcttgcccgc
     6661 gatgatcgac atgctcggcg gcggaatcgt cacgaccgcc gagacgcagc gcctgatcgg
     6721 ctccggcgcc ctcgatttca agggctatct cctttgcgtt ctcgtggtga tcgtggtggc
     6781 ctcgctctgc atgatcacgt cgcggctcgg ggtgatccgc gtcctcaaga attacgcttg
     6841 aggcttgtgg attgcggccg ttgcgcgcac taaagctagg tttcacgcat gcgcgatttg
     6901 tgcctattct tccgcccaac tggcacgtgc tggagtcggc ggtgagggcg gaacaggaag
     6961 agcggagagg ccggtttagc ggcttcttgg ggggcgtgcg gctcatcttc gatggcgtgc
     7021 tcttcctctt cgtgctgttc atcatcgggt tcatcgtctt tgcgaacggt atcgaccgca
     7081 agcagagcga gccgcaggag accgccgacg gcatcgccgt tctcaccggt ggcacatccc
     7141 gcatcgacag ggcgatgaag ctgctcgccg caggcaaggc caagcgcgtc cttatcaccg
     7201 gcgtcaaccg caccacgacc gtcgaggagc tgaagcagct cgcaagccaa ggcggccagt
     7261 acttcacctg ctgcgtcgac atcgacaagg aggcgcgcaa cacgatcgac aacgccacgg
     7321 agacgagcga gtgggtcgcg ctccaccgct atcgttcggt tatcgtcgtc acctccaact
     7381 atcacatgcc gcgcgcgctc gccgagcttg cccgggtgat gcccggcgtc accttcatcc
     7441 cctactcggt ggtcgacaac aacgtgcatc tcgaccgctg gtggacctat cccggcacga
     7501 ccaagctctt gctctccgag tacctgaaat atctgccggc gttggggcgg cttggcgcga
     7561 ccgagctcgt gcgcaggttt ctcggcggcg atcccctcac cggcgacgtt cccgagccgt
     7621 agcggtgtca cgcgagatgg cgcgctcgct gctgttcaac cttctgttct atgcgacgac
     7681 cgtgttgttc ctcgtgctcg ggagtcccct cctgttcggc ccgagacgct gggccatggc
     7741 cgctctcgcc atccacggtc gcttcgagct ttggctgctc gaacgcattg tcggcacgac
     7801 gctcgaagtg cgcgggctcg acaaggtgcc tgatggtgcc tgcctcgtcg ccgccaagca
     7861 tcagtcggca tgggagacct tcggcctcat cccgctgttc cgcgaccccg cgctcctgat
     7921 gaagcgcgag ctgttctgga tccccctgca cgggtggttc tcgcacaaat tcgagatgat
     7981 cccggtcgac cgcgacaagg gacccgcggc actgcggcgc atggtccgcg aggcgaagaa
     8041 gcgcgccgcc caagggcgcg agatcatcat cttcccggaa gggacgcggc gggcgcctgg
     8101 cgcgccgccc gactacaaga cgggcgtggt cctcctttac gaggcgctcg gcatcccttg
     8161 cgtgccgctc gccctcaatt caggcgcctt ctggccgcgc cggagtttcg tgcggcaccc
     8221 ggggattatc gtggtggaga tcctcgaccc aatcccgcct ggcctgccga aggccgagtt
     8281 cctgccgcgg ctgatcgcga cgatcgagac cgcctcggcg cggctcctcg cagaagcaaa
     8341 ggccgaggaa catggtgttg ggcggcctgt taacctttga atgcttgact agagccgcac
     8401 ccctgttcta cctttgttct tacgattcga ggccccgtct tgaattcctc gtcacggaac
     8461 ccatttcaca aagcgatcta ctggttaacg ggttgggatc ctatgtggac gcgcaaagtc
     8521 gaagttgaaa cggcgcaaat gggacttgcg gacgagccgg ccctggtgtg ctccgaagcc
     8581 gccttcgccc aggcctttca ctattggcgc ggtgcctccg gacggcgcta tctccacagc
     8641 gtctacagcc tcatcggctg ccctgctttg ccccgggcca actacattct cgttcgccgc
     8701 tacgacgacg ggacccgggc tgccttggcc tttggccaga ccaaggatga gacggcgagc
     8761 ctcaatctcg ctcatctccg ccatcaaggc gcgaaatgcg gagcgaacga ggtccatatc
     8821 catctcctcg ccgatacgcc cgatgcgcgt gccgaggtcg aggccgacct cctcgcggcc
     8881 catacgcgaa ggctcgccac cgccaacgcg gcttaagccc gagagcgcca agggtcggcc
     8941 gcaaaaccgc ccgatacgac ggcttcaacg tcaaatcgct gcatgctact gagacgggcc
     9001 aagcacccga gggcgtgctc gcagcgacgg gggaacctca tttggcaggg cttttcaaga
     9061 atccgtggct gcggcgccag aggcagcgcc tcaggcgccc cttgcaggtg ctcgcgagcc
     9121 ccgcgctcgc caactacatc aatcttgtcc tcaacacctc gaaccaaatc gccgatccgc
     9181 ctgacttcat cgcgcgggcg aaggcccaac acccccagat catcgccatg tggcacgggc
     9241 agtttcttct gctgcccggt atctatccgc cggagatccc gggccgcgcc atgatcgcgc
     9301 gccacgacga cgccgaggcc ctcgcccgcg tgaccgccgg cgccgacatg gcgcgggcca
     9361 cgcccgaaaa gcggcggccg gcgcaccgct gctcccaaaa cgctgccacg ggaagagcct
     9421 acgctccggc cggcaccggg acctgcttcc atccttgcgc tgacggaccg tctcataatc
     9481 atcgatcgcg gcggcgtcac cttacgccac caacctattc aggcaatttg tccaagtcct
     9541 gttgttggag atgccggctg cgcgtctcgg ccagcgtatc ccaagtgtcg gccatctcga
     9601 tcaatgaacg tttgtgatct ggattgctga cttgggcagc cattcttcgg cattcatccg
     9661 cctgagcctt gtattcagag agcttctgca cggcgtggcc tttcttcctt gctgttccag
     9721 tcgcaccacc tgtctggatg ctgcgccaac ggcggtcgat gatccagaca caatatttct
     9781 tgttttcgct caactcggca tggacttcga cagtttgccg ctgcccctac cctcaccaaa
     9841 ccttgccgca aaatcgattc ccaggtgcct cttcgtcgct atgttcgatc gctatgtgca
     9901 ggatcccggc ctcaaatctt cggcatcctc gggcaagcct tcaaatccaa agggcctagc
     9961 cggatgccga agataaatgg tcggggtgga gcaaagagga tttaaaagga ccactcgaac
    10021 gcgggccatt tcagctaggt cgcagcccct gccagtgcgc cagcgctggc actggctcgc
    10081 tcccaagagt agaatcggga aaataacgca gtccaacacc ttgttccggg aggcgctcgt
    10141 gtgcgcgcgc ttaggccctc gttttcggag aa
//
LOCUS       DASUQP010000257         6985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1488782, whole genome shotgun sequence.
ACCESSION   DASUQP010000257 DASUQP010000000
VERSION     DASUQP010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488782"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..2118
                     /locus_tag="VFY74_11745"
     CDS             100..2118
                     /locus_tag="VFY74_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5932401.1"
                     /translation="MNYYRLNQIAMAVLGALLLFFGARTVIQILQEEHEPEKPGYEVA
                     GTEKKKDEGKPDGGAQLASLLAGADAARGQEVAKKCAICHTFDKGGANLIGPNLFGVL
                     GAKMASHEGYEYSEALKAKGGVWDYANLDHMIENPNAFAPGTKMALFPGLPDAKQRAD
                     VILFLRSKNDSPPPLPEPTAAPAEEAPAAEAKAGSEVLALLATADPKQGEADAALCKV
                     CHTFDKGGAVLVGPNLYGIVGKKIASQEGVNYTAALKAKEGDWTYEHLDVWLTNPQAF
                     APGTTMAFPGVPDTKKRANIVAFMRGKADTPEPLPQAAPAADKAAPTEAAPPAAEAPA
                     APTEAAPPAAEAPAAPMEPAPPAAEAPAAPTEAAPPAAEAPAAPAGGAEIVALIATAD
                     PAQGEAEAALCKVCHTFDKGGATLIGPNLYDIVGKKIAAREGFTYSPALKAKEGDWTY
                     ETLDVWLANPQAFAPGTTMMLPAALDAKKRANVVAFMRSKSDSPVPLPDAAGEAAKPA
                     SKTPPAPTEPAPPAAETPPAPTEPAPPAAKTPPAPTEPAPPAAETPPAPTEAAPPAAE
                     TPPAPTEPAPPAAETPPAAAPEAPAATEPTPAAEPEPAAEPEPAVEAEPTPSEPSMGE
                     PPSPSQPQPVYPDVEPAATEDAAAEAPASMGEPPSPSQPQPVYPEGPP"
     gene            2408..4258
                     /locus_tag="VFY74_11750"
     CDS             2408..4258
                     /locus_tag="VFY74_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5932402.1"
                     /translation="MSLPRLALAVLLAGLLPYLALAEDAPRRHQLSLIGEPRYPADFK
                     HFDYVNPDAPKGGVVRLSDIGGFDSLNPVLYKGEAAGGIGLVVETLMSDNLDEPSTSY
                     GLIAEWASYPDDYSSVTFKLRDEARWHDGKKITPEDAIYSLEVNKAANPRVGLYYKNV
                     SRAEATGENEVTFYFDVKNNRELPMIMGQLTILPKHYWTGTDASGNQRDPMKTTLEPP
                     LGSGPYRIKDVKPGRSISYERVTDYWGKDLPVNAGQWNFDEIRFEYYRDETVAFESFK
                     AGNLDYWQETSAKNWATAYGFAAVKNGFVRRQEVPVERTHAMQCFVLNLRRPQFQDSR
                     VRQAFNLAFDFEWANKNLFYNQYARVGSYFEGGELAAPKALPEGRELEILNEVKDGVP
                     AEVFTEIHRNPVNDNPDAMRVNLRKAVTLLKEAGWEVKNGVLTNVKTGQQMKVEFLLV
                     SPLFERIVQPYMRNLERLGIKGTIRMVDSAQYTRRLDGFDYDIVVGNFPQSDSPGNEQ
                     RDFWGSEAAGREGSTNLIGVMNPAIDKLIDRIIFAKDRAELVAATRALDRVLLWNEFV
                     VPQWYSPNVRIAYWARYGQPKVLPGLTPGFLQVWWFDQTLAARLPGSSQL"
     gene            4258..6210
                     /locus_tag="VFY74_11755"
     CDS             4258..6210
                     /locus_tag="VFY74_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5932403.1"
                     /translation="MARLPSCRSIAGCAALALVLCAASASADERKQGLSAFGDLTYPA
                     DFKHFAYANPDAPKAGTFSLVGWGGVTTFNSLNNYILKGDAAQGLELLFDSLMTRAVD
                     EPDAVYGLVAESAEIAPDGKSVTFHLRPEARFADGTPLTADDVVFSFDALKTKGHPLF
                     HQMLQDVVKAEALDPVTVRYSFSGELVRDLPLTVATLPIFSKTYYASRPFDETTLEPP
                     LGSGPYVVDRVAQGRSIVYRRNPDYWAKDLAVNRGRWNFDKIRYEYFRDRTAGMEAFK
                     AGAYDFREEFTSKVWATEYDFPAIRAGKIRKEVLPDETPSGTQGFFLNIRREKLKDPR
                     VRKALDLAFDFEWTNRNVFYGLYDRTTSFFENSPMEAMGEPPEAERSLLTSLGVPVSE
                     EALGPAYSPAKSDGSGQDRNLLQQAGKLLDEAGFTVKNGVRVDAEGEPFTLEILTFEP
                     VFERVMAPYVKNLKLLGIDARIRMVDAAQYQQRLKDFDFDITTQRYVMRSTPGVELRS
                     YFGSDAAKMDGSLNLAGIADPAVDALIERILAAKSREELETAARALDRVLRVGHYWVP
                     HWYKASNNVAYWDKFSRPGTKPRFDRGILDTWWYDEAKAAKLAQAGAAPADSTGRSPW
                     LILAALIGLMLAVYFVARLRRSARTE"
     gene            6207..>6985
                     /locus_tag="VFY74_11760"
     CDS             6207..>6985
                     /locus_tag="VFY74_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5932404.1"
                     /translation="MIAYILRRLLLIIPTMFGIMLVTFVIVQFAPGGPVERLIAQLTG
                     TDPGATSMFSGGGGDFAGKPGQGAAPGDVSAPSKYRGAQGLDPEFIKNLEKQFGFDKP
                     AYERFFLMVKNYLLFDFGESYFRDTSVLGLIAEKLPVSISLGLWMTLLSYAISIPLGI
                     RKAVHDGSRFDIWTSTLIVISYAIPGFLFAVFLIVLFAGGSFLQIFPLRGLVSDNWWE
                     LPWYLKIADYFWHLVLPLIAMALSAFATTTFLTKNSFLEEIR"
BASE COUNT         1314 a         2339 c         2143 g         1189 t
ORIGIN      
        1 aaatgtccga gttgcaacaa gcgtgtaggc cctatagtct cccgccccgc cgcaagagct
       61 acatggaagt agcctctctg cttgggggga ccctcgatca tgaactatta ccgcttgaat
      121 cagatcgcca tggcggtgct cggtgcgctg ctgctgttct tcggcgcgag gaccgttatc
      181 cagatcctcc aggaggaaca tgagccggaa aagcccggct acgaggtcgc gggcaccgaa
      241 aagaagaagg acgaaggaaa accggacggc ggtgcccagc ttgcctcgct tcttgcgggc
      301 gcagatgcgg cgcgcggcca agaggtcgcc aagaaatgcg ccatctgtca caccttcgac
      361 aaaggcggcg ccaacctgat cggtcccaat ctctttggcg tgctcggcgc taaaatggcg
      421 agccacgagg gctacgaata ttccgaggcg ctaaaagcaa aaggcggcgt gtgggactat
      481 gccaatctcg atcatatgat cgagaacccc aacgcgtttg cgcccggcac caagatggcg
      541 ctgtttccgg gccttcccga tgccaagcag cgggccgacg tgattctgtt cttgcgcagc
      601 aagaacgaca gtcccccgcc tttgcctgag cccacggccg ctcccgccga ggaggcgccg
      661 gccgcggaag ccaaggcggg ttcagaggtt ctcgcgctcc tcgccacggc cgacccgaag
      721 cagggtgagg ccgacgcggc gctctgcaag gtgtgtcaca ccttcgacaa gggcggcgcc
      781 gttctggtcg gcccaaatct ctatggcatc gtgggcaaga agatcgcttc gcaagagggc
      841 gtcaactaca cggccgcgct caaagccaag gaaggcgatt ggacctacga gcatctcgat
      901 gtctggctga ccaatccgca ggccttcgcg cccggaacga cgatggcctt ccccggcgtg
      961 cccgacacga agaagcgcgc caacatcgtc gcctttatgc gcggcaaagc cgacacgcca
     1021 gagccgttgc cgcaggctgc tccggcggca gacaaggccg cgcctacaga ggcggcgcct
     1081 cccgcggcgg aagcaccagc ggctcccacg gaggccgcac ctccagcggc ggaagcacca
     1141 gcggcaccta tggagccggc acctccagcg gcggaagcac cagcggcgcc gacagaggcg
     1201 gcacctcccg cggcggaagc accggcggcg cccgcgggcg gagcagagat cgtcgcgctg
     1261 atcgccacgg ccgacccggc acagggcgag gccgaagcgg cgctctgcaa ggtctgccat
     1321 accttcgata aggggggcgc cactctgatc ggccccaatc tctacgacat tgtcgggaag
     1381 aaaatcgccg cacgggaagg gttcacttac tcgcctgcgc tcaaggccaa ggaaggcgac
     1441 tggacctacg agaccctcga tgtgtggttg gcaaacccgc aggccttcgc gcccggcacg
     1501 accatgatgt tgccggctgc gctcgacgcc aagaagcgcg ccaacgtcgt cgccttcatg
     1561 cgcagcaaga gcgacagccc ggtaccgctg cccgacgctg ccggcgaagc ggccaagccc
     1621 gcgagcaaga caccgccggc cccgactgaa cccgcccctc ccgcggccga gacaccgccg
     1681 gccccgactg aaccggcccc tcccgcggcc aagacaccgc cggccccgac cgaaccggcg
     1741 cctcccgcgg ccgagacacc gccggcgccg actgaggcgg cgcctcccgc ggctgagaca
     1801 ccgccggccc cgactgaacc ggcgcctccc gcggccgaga caccgccggc ggcagcgccc
     1861 gaagctccgg cggcaactga accgacaccc gcggcagaac cagaacccgc ggcagaaccg
     1921 gagcccgcgg tagaagcgga gccgacgccg tccgagccct cgatgggcga gccaccctcc
     1981 ccctcgcagc cgcagccggt ctatcccgat gtggaaccgg ccgcgaccga agacgccgcg
     2041 gctgaggcgc cagcatcgat gggcgagcca ccctccccct cgcagccgca gccggtctat
     2101 cccgagggcc cgccgtaggc aggtgtcagc tatcaggtgt cagaaatcag aaacagaaaa
     2161 tcaataacgc tctggcacct gcctcctgcc acatcgtacc tggcaccagc cccacgactc
     2221 cttctgattt ctgacacctg atacctgaca cctgacacct ggcacctgat tcctgaccga
     2281 taattcattt ggccgaaggg cccgccatgc taggttttgg cggcttttct tcgcctttcg
     2341 ccccctcctg cgggggacgg tttcgcgccc aagccattcc attgaggtgg agcccatgcc
     2401 gccgctcatg agtttgcctc gcctcgcgct tgcggtcctt ctggccggcc tccttccata
     2461 tctcgcactg gccgaggacg cgccccgccg ccaccagctc tcgctcatcg gggagccgcg
     2521 ctacccggcc gatttcaagc atttcgatta tgtgaatcct gacgcgccca aggggggcgt
     2581 cgtgcgccta agcgatatcg gcggtttcga tagtctcaac ccggtgctct acaagggcga
     2641 ggcggcggga gggatcggcc tcgtggtcga aaccctgatg tccgacaatc tggatgagcc
     2701 atcgacgtct tacgggctga tcgccgaatg ggcgtcatac ccggacgatt actcctcggt
     2761 cacgttcaag ctgcgcgacg aggcgcggtg gcacgacggc aagaagatca cgcccgagga
     2821 cgccatctac agcctcgagg tcaacaaggc cgccaacccg cgcgtgggcc tctattacaa
     2881 gaacgtgtcg cgggccgaag ccacgggcga gaacgaggtc accttctatt tcgacgtcaa
     2941 gaacaaccgc gagctgccga tgatcatggg gcaactcacg atcctgccca aacattactg
     3001 gaccgggacc gacgcgagcg gcaatcagcg cgatccgatg aagacgacgc tcgagccgcc
     3061 gctcggctca ggcccttacc gcatcaagga cgtcaagccc ggacgatcga tcagctatga
     3121 gcgggtgacg gactattggg gcaaggatct gccggtgaac gccgggcaat ggaacttcga
     3181 cgagatccgc ttcgagtact accgggacga gacggtcgcc ttcgagagct tcaaggccgg
     3241 gaacctcgac tactggcagg agacgagcgc caagaattgg gcgacggctt acggtttcgc
     3301 tgcggtgaaa aacggtttcg tccggcgtca ggaagtcccg gtcgagcgca cccatgccat
     3361 gcaatgcttc gttctcaact tgcggcgtcc gcaattccag gacagccgcg tgcggcaagc
     3421 cttcaatctc gctttcgact tcgagtgggc caacaagaac ctgttctata accaatatgc
     3481 gcgtgtcggc agctatttcg aaggcggcga gctcgcggcg cccaaggcgc tgcccgaagg
     3541 ccgcgagctc gagatcctga acgaggtgaa agacggggtg ccggcggaag tgttcaccga
     3601 aatccatcgc aacccggtga acgacaatcc cgatgcgatg cgcgtcaatc tgcgcaaggc
     3661 ggtgacgctg ttgaaggagg cgggctggga ggtcaagaac ggcgttctca ccaacgtcaa
     3721 gaccggccaa cagatgaaag tggagttcct gctcgtgtcg ccgctgttcg agcgcatcgt
     3781 gcagccgtat atgcgcaacc tcgagcggct cggcatcaag ggcacgatcc gcatggtcga
     3841 ttcggcgcag tacacgcggc ggctcgacgg gttcgactac gacatcgtgg tcggcaattt
     3901 cccccaatcc gactcgcccg gaaacgagca gcgcgacttc tggggatcgg aggccgccgg
     3961 ccgcgaaggc agcaccaatc tcatcggcgt gatgaacccg gcgatcgaca agctgatcga
     4021 tcgcatcatc ttcgccaagg atcgcgccga gcttgtcgcc gccacgcgcg ctctggaccg
     4081 cgtgctcttg tggaacgagt tcgtggtgcc gcaatggtat tcgccgaacg tgcgcatcgc
     4141 ctattgggcc cgctacggcc agcccaaggt gttgccgggg ctcacgccgg ggttccttca
     4201 ggtctggtgg ttcgaccaga cgctcgccgc gcggctgccg ggctcctcgc agctctgatg
     4261 gcgcgcctcc cgtcctgccg gtcgatcgca ggctgcgccg ctctcgctct tgttctctgt
     4321 gccgcttcgg cgtccgccga cgagcgcaag caggggcttt cagccttcgg cgacctcact
     4381 tacccagccg atttcaagca tttcgcctat gccaatccag acgcaccgaa agccggcacc
     4441 ttctcgctcg tcggctgggg cggcgtgacg accttcaaca gtctcaacaa ctatatcctc
     4501 aagggcgatg cggcgcaggg gctcgagctc ctgttcgatt cgctgatgac gcgggcagtc
     4561 gacgagcctg acgcggtcta tgggctcgtg gccgaaagcg ccgaaatcgc acccgacggc
     4621 aagtcggtca cgttccatct gcgccccgag gcgcgcttcg ccgacggaac accgctcacc
     4681 gccgacgacg tggtgttctc cttcgacgcg ctcaagacga aaggccaccc gctcttccac
     4741 cagatgctgc aagacgtggt caaagccgag gccctcgatc ccgtcaccgt gcgctacagc
     4801 tttagcggcg agctggtgcg agacctgccg ctcaccgtcg ccacgcttcc catcttctcc
     4861 aagacctatt atgcgagccg gccgttcgac gagaccacgc tcgagccgcc gctcggctcg
     4921 ggcccttacg tcgtcgatcg ggtggcgcag gggcggagca tcgtctaccg ccgcaacccg
     4981 gactattggg cgaaggactt ggccgtcaac cgcgggcggt ggaacttcga caagatccgc
     5041 tacgaatatt tccgcgatcg caccgcgggg atggaagcgt tcaaggccgg cgcctacgac
     5101 ttccgcgagg agttcacctc gaaggtctgg gcgaccgagt atgactttcc ggcgatccgg
     5161 gcgggaaaga tcagaaaaga agtgcttccc gacgagacgc cctcgggaac gcaaggtttc
     5221 ttcctcaaca tacggcggga gaagctgaag gacccgcgcg tgcgaaaggc gctcgatctt
     5281 gccttcgact tcgagtggac caatcgcaac gtcttctatg gactctatga ccgcaccacg
     5341 agcttcttcg agaattcgcc gatggaggcc atgggcgagc cgccggaggc cgagcgctcg
     5401 ctgctgacga gcctcggcgt gcccgtctcg gaggaggcgc tgggtccggc ttactctcct
     5461 gccaagagcg acggttcggg ccaggaccgc aatctccttc agcaagcggg gaagcttctc
     5521 gatgaagcgg ggttcaccgt caagaacggc gttcgcgtcg atgccgaggg cgagccgttc
     5581 acgctcgaga tcctgacatt cgagccggtc ttcgaacggg tgatggcgcc ctatgtgaag
     5641 aatctcaagc tgctcggaat cgatgcgcgc atccgcatgg tcgatgccgc gcaataccag
     5701 cagcgcctca aggatttcga cttcgacatc accacgcagc gctacgtcat gcgcagcacg
     5761 ccgggcgtcg agctcaggag ctatttcggc tcggacgcgg ccaagatgga cggttcgctc
     5821 aatcttgccg gcatcgccga tcccgcggtg gatgcgctga tcgagcgcat tctcgccgcc
     5881 aagagccgcg aagagctcga gaccgcggca cgcgcgctcg accgcgtgct cagggtcggt
     5941 cactactggg tgccgcattg gtacaaggcc tcgaacaacg tggcctattg ggacaagttc
     6001 tcgcggcccg gaaccaagcc gcgcttcgat cgtggcatcc tcgacacgtg gtggtatgac
     6061 gaggccaaag ctgccaaact ggcccaagcc ggcgccgcgc cggccgattc gaccggccgg
     6121 agcccgtggc tcatcctcgc cgccctgatt gggcttatgc tcgcggtcta ttttgtcgcg
     6181 cgattgagaa ggtccgcccg aaccgaatga tcgcctatat ccttcgccgc ctcctgctga
     6241 tcatcccgac gatgttcggc atcatgctcg tgaccttcgt catcgtgcag ttcgcgccgg
     6301 gcggcccggt cgagaggctg atcgcgcagc tcaccggcac cgatcccggc gccacctcga
     6361 tgttcagcgg cgggggcggc gacttcgcgg gaaagcccgg gcaaggggcg gcgcccggcg
     6421 atgtgtcggc gccgtcgaaa tatcgcgggg cgcaagggct cgaccccgaa ttcatcaaga
     6481 acctcgagaa gcagttcggc ttcgacaagc cggcctatga acgcttcttt ttgatggtca
     6541 agaactacct tctgttcgat ttcggcgaga gctatttccg cgatacgagc gtgctcggac
     6601 tgatcgccga gaagctgccc gtctcgatct cgctcggtct ttggatgacg ctgctttcct
     6661 atgccatctc gattccgctc ggtattcgca aggcggtgca cgacggctcg cgcttcgaca
     6721 tctggaccag caccctcatc gtcatcagct atgccatccc cggctttctc ttcgccgtat
     6781 ttttgatcgt gctgttcgcc ggcggatcgt tcttgcagat cttcccgctg cgaggcctcg
     6841 tatccgacaa ttggtgggag ctgccctggt acctgaagat cgccgattat ttctggcatc
     6901 ttgttctgcc gctgatcgcg atggcgctgt cggccttcgc caccacgacg ttcctcacca
     6961 agaactcgtt cctcgaggag atccg
//
LOCUS       DASUQP010000258         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1081234, whole genome shotgun sequence.
ACCESSION   DASUQP010000258 DASUQP010000000
VERSION     DASUQP010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081234"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /gene="trmD"
                     /locus_tag="VFY74_11765"
     CDS             <1..607
                     /gene="trmD"
                     /locus_tag="VFY74_11765"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5932405.1"
                     /translation="ALDLIDIRDFATDKHRSVDDTPAGGGAGMVMRADIAAAAIDAAR
                     ARAETGTPALYLSPRGRLLKQGQVRALAQGPGAILLCGRFEGIDQRVLDTRQIEEISI
                     GDYVLSGGELAAQVLIDACVRLLPGVAGNESSLAEESFASGLLEYPHYTRPREWEGCA
                     IPEVLLSGDHKKIAEWRREQADKLTKERRPDLWDRRDKRKL"
     gene            914..1273
                     /locus_tag="VFY74_11770"
     CDS             914..1273
                     /locus_tag="VFY74_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932406.1"
                     /translation="MTWSMRWALCASLICAVPAMAQVKMEHLLSTYSTIEDGCRDEST
                     QDFEIRRGIVEGPNVLCILGAPKEASAGQEAYEAKCREKGEVRLGKVTFDLSGKPERM
                     RITLPESQDWITLYACR"
     gene            1456..1854
                     /gene="rplS"
                     /locus_tag="VFY74_11775"
                     /pseudo
     CDS             1456..1854
                     /gene="rplS"
                     /locus_tag="VFY74_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            2316..3722
                     /gene="leuC"
                     /locus_tag="VFY74_11780"
     CDS             2316..3722
                     /gene="leuC"
                     /locus_tag="VFY74_11780"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5932407.1"
                     /translation="MASPRTLYDKIWDAHLVHEAPDGTCLLYVDRHLVHEVTSPQAFE
                     GLRMAGRNVRAPEKTLAVVDHNVPTTDRSHGIDDPESALQIATLADNAREFGIEYYDE
                     LDHRQGIVHVVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQQKAKNMRVRVDGALPEGVTAKDIILAIIGEIGTAGGNGHVIEYGGEAVRALSMEGR
                     MTICNMSIEGGARAGLIAPDAKTFDYIKGRPRAPKAGAFELARADWERLYSDEGAEFD
                     REVVLHAERLPPLVTWGTSPEQVVSVTGRVPIPAEIADDTKRRAAERSLDYMGLTGGE
                     KITDIALDRVFIGSCTNGRIEDLREVAKVVEGRKVNERIRAMIVPGSGLVKAQAEAEG
                     LDKIFKDAGFEWREPGCSMCLGMNPDQLAPRERCASTSNRNFEGRQGRLGRTHLVSPQ
                     MAAAAAIAGRFVDIRNFH"
     gene            complement(3844..>4025)
                     /locus_tag="VFY74_11785"
     CDS             complement(3844..>4025)
                     /locus_tag="VFY74_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932408.1"
                     /translation="ASHYRYPGYGFHFGYGPRYRGGYGGGHYAYRHGQHGNWDGKRDR
                     GHHDRDGGGHYDRDR"
BASE COUNT          743 a         1307 c         1365 g          610 t
ORIGIN      
        1 ggcgctcgac ctcatcgaca tccgcgactt cgccaccgac aagcatcgct ccgtcgacga
       61 cacgccggcg ggaggcggcg ccggcatggt gatgcgcgcc gacatcgccg ccgctgcgat
      121 cgacgccgcc cgcgcgcgcg ccgagaccgg gacgcctgcc ctctacctct ccccgcgcgg
      181 gcgccttctc aagcaaggcc aagtgcgtgc gctcgctcaa ggtcccggcg cgatcctgct
      241 ctgcggccgc ttcgagggga tcgaccagcg cgtgctcgat acgcgccaaa tcgaggaaat
      301 ctccatcggc gactacgtcc tgtcgggcgg cgagcttgcc gcgcaggtgc tgatcgacgc
      361 ttgtgtgcgg ctcttgccgg gcgtcgccgg caatgagagt tcgcttgccg aggaaagctt
      421 cgcatcgggc ctgcttgaat atccgcacta cacgcggccc cgcgagtggg agggctgcgc
      481 gatccccgag gtgctgctct ccggcgacca caagaaaatc gccgagtggc ggcgcgagca
      541 ggcggataag ctgaccaagg agcgccgccc cgatttgtgg gaccgccgcg acaagcgaaa
      601 actttgatcg cctttgctct ccccttgcgg gaacagtacg cacccgtcat gcccggacac
      661 ccgggtccgg cttcgccggc ccgggcgcga gcttgtccgg gcatccatgc cgtttcgtct
      721 cggcaagatg aagcgccaat ggaatggatt gccgggcctt cgcccgccga agcgatggct
      781 ggcgggagtg gccacgaggc ttcggcccgc gtaggcgggt caagcccggc aatgacgcag
      841 gacttggatt gactctcgcc tgcggagcgt tgtcgcctat attcgcagcc tcgagcgcgg
      901 ccaggagaga gcgatgactt ggagtatgcg ttgggcgttg tgcgcgtccc tcatctgcgc
      961 cgttccagcc atggcgcaag tcaagatgga gcacttactc agcacatatt ccacgatcga
     1021 agacggctgc cgcgacgaat ccacccagga tttcgagatc aggcgaggca tcgtcgaggg
     1081 gccgaacgtc ctctgcatcc tcggcgcgcc gaaagaggcg agcgcggggc aagaggccta
     1141 tgaggccaaa tgcagggaga aaggcgaggt gcgcctgggc aaggtgacct tcgacctctc
     1201 cgggaagccc gagcggatgc ggatcacgtt gcctgagagc caggactgga tcacgctcta
     1261 tgcatgccgc tgagcggctt gcccggcgcg cgcaaatcca tcgacaaggg cggaaagcta
     1321 ggctaggaag cgggccgtgc gcgggcgctt cccgatgctt cgcgcgctca aggactcttg
     1381 aggatcaatc aaaaagaatg gcgttcgccg gaaggctttt gagccgaggc aggcgcccac
     1441 cgaaaggtat ttgccatgaa tatcatcgag cagctcgaca aagagcaggc tgccgcgctc
     1501 tccaagaaac gcgaagtccc cgagttcggt cccggcgaca cgttgcgggt caacgtcaag
     1561 gtggtcgagg gcgagcgcac gcgcgtgcag gcctatgagg gtgtatgcat cgggcgctcg
     1621 ggcggcgggg tgaacgagaa tttcaccgtg cgcaagattt cctacggcga gggcgttgag
     1681 cgcgtctttc cggtccattc gccgctgatc gagtcgatcg aggtggtgcg gcggggacgc
     1741 gtgcgccggg ccaagctcta ttacttgcgc ggccggcgcg gcaaggcggc ccgtatcgcc
     1801 gaacgtcagg agttcaaggg caagaaggcc gaggcgttca agggcttcaa gaggccgaaa
     1861 ggcgagcccg acgatctcaa gctgatcccc ggcgtttcgg cggacctcga gaagaagctg
     1921 aacgagctcg gcctgatcaa gttcgagcag atcgccaatc tctccgacga cgagattggc
     1981 tggctcgacg aggagctgtc gctgaagggc gccatcgagc atgacgactg ggtggccaag
     2041 tccgtcgcgc tgatggccga gaccacggca ggcgaagtcc ccgccgagga agaggaggag
     2101 gccgaaggcg ccggcgaaac cgacgccaag gccgcaaagg cgaaaaaggg cgagaaggcc
     2161 gctaaagcca aagcgaccaa agaggccaag gccaagcctg ccaaaaagcc caagaagggc
     2221 tagcgctacc cccctgccgg ccccccgggt tggctcaggg atttgtaaac cgggcgccga
     2281 gacgttatct ctgcctctag atcgcgaccc ttgccatggc cagcccgcgc accctctacg
     2341 ataagatttg ggacgcccat ctcgtgcatg aggcgcccga cggcacgtgc ctcctctatg
     2401 tcgaccgcca cctagtgcac gaggtgacga gcccgcaggc cttcgagggc ctgcgcatgg
     2461 cggggcggaa cgtccgcgcg cccgaaaaga cgctcgccgt ggtcgatcac aacgtgccga
     2521 ccaccgaccg cagccacggc atcgacgatc ctgaatcggc gctccagatc gcgacgctcg
     2581 ccgacaacgc acgcgaattc ggcatcgaat attacgacga gctcgaccac cgccagggca
     2641 tcgtgcatgt ggtcgggccc gagcagggat tcactctgcc cggcaccacc atcgtctgcg
     2701 gcgacagcca cacctcgacc cacggcgcgt tcggtgcgct cgcccacggc atcggcacga
     2761 gcgaggtcga gcacgtgctc gccacccaaa cgctgatcca gcagaaggcg aagaacatgc
     2821 gggtgcgcgt cgacggcgcg cttcccgaag gcgttaccgc caaggacatc atcctcgcca
     2881 tcatcggcga gatcggcacc gccggcggca acggccatgt catcgaatat ggcggcgagg
     2941 cggtccgcgc gctctcgatg gaagggcgca tgaccatctg caacatgtcg atcgagggcg
     3001 gcgcccgggc aggcctgatc gcgcccgacg ccaagacctt cgactacatc aagggccggc
     3061 cgcgcgcgcc gaaggccggc gccttcgagc ttgcgcgagc ggactgggag cgtctctatt
     3121 ccgacgaggg cgcggagttc gaccgcgagg tcgtgctcca tgccgagcgg ctgccgccgc
     3181 tcgtcacctg gggcacgagc cccgagcagg tcgtatcggt caccgggcgc gtccccatcc
     3241 ccgcggagat cgcggacgac accaagcggc gcgcggccga gcgctcgctc gactatatgg
     3301 ggctcaccgg cggcgagaag atcaccgaca tcgcgctcga ccgcgtgttc atcggctcgt
     3361 gcaccaatgg ccgcatcgag gatttgcgcg aagtggcgaa ggtggtcgag ggccgcaagg
     3421 tcaatgagcg catccgcgcc atgatcgtgc ctggctccgg gctggtgaag gcgcaggccg
     3481 aggcggaagg cctcgacaag attttcaagg atgcgggctt cgagtggcgc gagccgggct
     3541 gctcgatgtg ccttggcatg aatcccgatc agcttgcgcc gcgcgagcgc tgcgcctcga
     3601 cctcgaaccg gaatttcgag ggacggcagg gtcggctcgg acgcacccat ctcgtctcgc
     3661 cgcagatggc tgccgccgcc gcgatcgccg gccgcttcgt cgatatccgc aatttccatt
     3721 aagcgccgga cacgtcatcc ttggggaggg ccgcggggcg agggcgacgc gtccgccgag
     3781 aatgacgtgc cggttacgcg ggagagcggg agggttactc tcccccgcgc ctagggagac
     3841 gcgtcagcgg tcgcggtcgt aatggccgcc gccgtcgcgg tcatgatggc cgcggtcgcg
     3901 ttttccgtcc caattgccat gctggccatg ccggtaggcg tagtgtccgc cgccatagcc
     3961 gccccggtaa cgcgggccgt agccgaagtg gaagccgtag cccggatagc gataatggct
     4021 ggcgc
//
LOCUS       DASUQP010000259         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1627014, whole genome shotgun sequence.
ACCESSION   DASUQP010000259 DASUQP010000000
VERSION     DASUQP010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627014"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..700
                     /locus_tag="VFY74_11790"
     CDS             287..700
                     /locus_tag="VFY74_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932409.1"
                     /translation="MSEQELMEKSDLVALVRVMSVTCTSLTKDAATGEDLPGYIAQLK
                     VLEVKKGDAKKGDEVLVTWRAVPKSILGPWTVYYYPGEEVVTHLTKRSGGASYASTWW
                     NAKGDDVKAPDTRDLPVKPGESVVAQPEKQKQTPL"
     gene            complement(1158..1847)
                     /locus_tag="VFY74_11795"
     CDS             complement(1158..1847)
                     /locus_tag="VFY74_11795"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX5932410.1"
                     /translation="MKNAIGIRHVAFEDAGILAGLLQDRGIGLRYLEAGVDDLSPAKD
                     ADLLLVLGGPIGIYEVARYPFLKEELAIIETAVKKDVPTIGICLGCQALAAVFAARVY
                     PGREKELGWDEMTLTKEGKASPLGAIEGVRVLNWHGDTFDLPVGATRLASTRITPNQA
                     FAYGPRVLALQFHVELPERDMEKWLIGHTMELTSSNADLGKIRAETARFAAAANAAGK
                     RLFENWLDALP"
     gene            complement(2176..>2654)
                     /locus_tag="VFY74_11800"
     CDS             complement(2176..>2654)
                     /locus_tag="VFY74_11800"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5932411.1"
                     /translation="GNGFIVGKFGFMLNNMLGEEDLVRGGLDAWRPGTRLSSMMAPTI
                     ILEPDGTTTALGTGGSNRIRSAILQVAANLLDSGMSLEEAVEAARLHVERDGTVSFEP
                     GLPAQAARAFAALGAKAHAWPERNLFFGGVHAARRDCNGDVDGAGDPRRGGLSRVV"
BASE COUNT          444 a          878 c          873 g          459 t
ORIGIN      
        1 cgatgaagcc gcacattcgg ggcttccccg ccccggctcc gtcatggccg ggcctcgccc
       61 ggccatccac gattgactct tttccactag cctaaatcgt ggatgcccgg catgaagccg
      121 ggcatgacga actcctttag aacgtcttag tcttctcgca gttcaagatc gggcaataaa
      181 ctcgaaatcc ctacaggcaa gttccatgcg cctttgcatc atactttccg ccttgtgtct
      241 ctcctttagc ctggcgagcg ggcgggcgcg cgccctgccc gcgccgatgt ccgaacagga
      301 gctgatggag aagagcgatc tcgtggcact cgtccgcgtg atgtcggtga cctgcacgag
      361 cctcaccaag gacgccgcga ccggcgagga ccttcccggc tatatcgccc agctcaaagt
      421 gctcgaggtc aagaagggcg acgcgaagaa aggcgacgag gtgctggtga cctggcgcgc
      481 cgtgccgaag tcgatcttgg gcccgtggac cgtctactac tatccgggcg aggaggtcgt
      541 gacccatctc accaagcgga gcggaggcgc aagctatgcc tcgacctggt ggaacgccaa
      601 gggcgacgac gtgaaagcgc cagacacgcg ggacttgccg gtgaagccgg gcgaatcggt
      661 cgtggcgcag cccgagaagc aaaagcagac cccactctag gaaggtggaa tcaacctcgc
      721 cttgatgggg aggtcggcgc gctgctttat ttttgcgcta ccgctccgct gcttgagcgc
      781 acagcgcgcc gggtggggtg atgctctcgg cgcgccccac ccgcctcgcc aaccgacttg
      841 gcttcgccaa gtcggggcgt gccgccctcc ccacgttggg ggagggatga gtcttaatgc
      901 ggagtagatc ttcccctctc cctcgaggga gagcttcccg cccggcgcgc gaagcgcgac
      961 gagtggcggg aagggtgagg ggtgagtctt ttttgacgcg ggctttcgcc cgcttacccc
     1021 tcaccgcgcc gcaagcggcg cgtgctcgcc cacaacctgc ctgcgcgaag ccggggcttc
     1081 gctccggcgg aggcaggggg gagagccgga gttcgtagcg atttttgggg aatcaaaccg
     1141 agcgcatgcc gctctcgtca ggggagcgcg tcgagccagt tttcgaacag gcgcttgccg
     1201 gccgcgttcg cggcggcggc gaagcgcgcg gtctcggcgc ggatcttgcc gaggtcggcg
     1261 tttgagctcg tgagctccat ggtgtggccg atcagccatt tctccatgtc gcgctccggc
     1321 agctcgacgt ggaattgcag cgcgagcacg cgcgggccat aggcgaaggc ctggttcggc
     1381 gtgatcctgg tcgaggcaag acgcgtcgcg ccgacgggga gatcgaacgt gtcgccatgc
     1441 cagttcagca cgcggacgcc ttcgatcgcg ccgagcggcg aggccttgcc ttctttcgtc
     1501 agcgtcatct cgtcccagcc aagctccttc tcgcggccgg gatagacgcg cgccgcaaac
     1561 acggcggcaa gcgcctggca gccgaggcag atgccgatcg tcggcacatc cttcttgacc
     1621 gcggtctcga tgatggcgag ttcctctttc aggaacggat agcgggcgac ctcgtagatg
     1681 ccgatcgggc ctccgagcac gaggagcagg tcggcgtcct tggccgggct gagatcgtcg
     1741 acgcccgctt cgagatagcg gaggccgatg ccgcggtcct gaagaagccc cgccaaaatg
     1801 ccggcatctt cgaaggcaac gtggcggatg ccgatggcgt tcttcatggc tccctctcac
     1861 tcaggctcat ggcggtattg tgcggagcca cgccccggcc gtgttgacaa ttaacgacgc
     1921 gcgcttgacc ttttacgaca agtgccgact cgtcccccgc gccacacatt cccctctccc
     1981 cttgggggga gagggcttca cgccgcgatc ttgcgcggcg aagcctgcgc aaagtcgctt
     2041 ggcgggaagg gtgaggcgtg aatccttttg acgcgggctt acgcccgctt acccctcacc
     2101 gcgcaccaag tggtgcgcgt actctccccc aaggggagag cggggacact cttgctgcgc
     2161 ttcagagcga actcatcaca cgacgcgcga cagtccgccg cggcggggat cgcccgcccc
     2221 gtcaacgtcg ccattgcaat cgcggcgcgc ggcgtgcacg ccgccgaaga agaggttgcg
     2281 ctcaggccag gcatgcgcct tcgcgccgag cgccgcaaat gcgcgcgcgg cctgcgccgg
     2341 caggccgggc tcgaagctga ccgtgccgtc gcgctcgaca tgaagcctcg ccgcctcgac
     2401 cgcctcctcg aggctcatgc cgctgtcgag gaggttggcg gcgacctgga ggatcgccga
     2461 cctgatgcgg ttcgagccgc cggtgccgag cgccgtggtc gtgccgtcgg gctccaggat
     2521 gatggtcggc gccatcatcg aggaaaggcg cgtccccgga cgccaagcat cgagccctcc
     2581 acgcaccaag tcctcctcgc ccagcatgtt gttcagcatg aaaccgaact tgcccacgat
     2641 gaagccgttg ccct
//
LOCUS       DASUQP010000260         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_810923,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000260 DASUQP010000000
VERSION     DASUQP010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810923"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..380)
                     /locus_tag="VFY74_11805"
     tRNA            complement(305..380)
                     /locus_tag="VFY74_11805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Lys,seq:ttt)
     gene            529..804
                     /locus_tag="VFY74_11810"
     CDS             529..804
                     /locus_tag="VFY74_11810"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX5932412.1"
                     /translation="MSNGKARPRLLRFWPRLLFLVPFVAMLWVASYNRTAPALDGIPF
                     FYWYQLGWILAGAAIVLLVYWIESKVTRVTGRTEPEAEPSGVPGDIL"
     gene            801..2324
                     /locus_tag="VFY74_11815"
     CDS             801..2324
                     /locus_tag="VFY74_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX5932413.1"
                     /translation="MNLDLTATIVFVGLFLFVTWLGFVAARWRKADLTLLHEWGLGGR
                     RFGTTVTWFLLGGDLYTAYTFIAVPALVYGKGAMGFFAVPYTILIYPILYIVFPRLWE
                     ITRARGHVTAADFVRDRFGSRLLALAFALTGIVATMPYIALQIVGMEVVIGALGFPAG
                     GFVGHLPLIISFIILAAFTYTSGLRAPALIAIVKDMLIFVTVFAALVIIPAELGGYGA
                     IFRSVPQEKLILPPPSGDSLGAYSAYATLALGSAFALFLYPHAMTGILSSSSGNVIRR
                     NAAFLAAYSFILGLIALLGYMAIAAGVDTNPAYQAGFAQYGPNFAVPALFLASFPSWF
                     VGVAFAAIAIGALVPAAIMSIACANLFTRNLYKEFYRPDCSEADEARMAKIVSLVVKI
                     GAVVFILAIPQSYAIQLQLLGGIWIIQLLPPILLGLYTRGFNAMALLAGWVAGTVIGT
                     YMAATQGFTSSVYPLEAFGLTVPGYAALYSVLINFMVAFALAPIFGRIGRTTGATAP"
     gene            2415..>2630
                     /locus_tag="VFY74_11820"
     CDS             2415..>2630
                     /locus_tag="VFY74_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932414.1"
                     /translation="MADDNQGGRTFLAIVLGGIVLALVIAALFMYGHKGESDVKVEKP
                     MTEQPAETPAPPPAAEMPAEPPPAEMPA"
BASE COUNT          411 a          885 c          774 g          560 t
ORIGIN      
        1 tgacccgcta agctaaagca cgtgcgttca gattagttcc ttcactgtcg cgtgccgcaa
       61 gagccttgcg ccgatgactt ggttcccatc gaaggtgcgc gatgcggtga gaaagcggat
      121 gggtaaagcc gcctccaagg aaactgcccc gcccaacggc accttcccga ccgagggcgc
      181 aacgcacaac gcactaactg agcagcggca ccgcctcccg cgacgaatgc ttgcgggttt
      241 gttatcctcc tgttatcccg gatcttcgag cttcgtctag aaaaagcgta agccattgaa
      301 actatggtga gcccgccggg attcgaaccc gggacgcctt gattaaaagt caagtgctct
      361 accggctgag ctacgggctc cttgccgacc atatgggccg gtcggcggcc aaagcaatat
      421 tgcgcctcgc cttgctaaac aacttaaaat gcggcatccc cgctcgatcc gaggctgagg
      481 cgcctgcggc gccgtggtgg tgcgccgggc aagtggggaa ccgaaccaat gtcaaacggc
      541 aaggcaagac cgcgcctgct gcgcttttgg ccgcgcctgc ttttcctcgt tcccttcgtc
      601 gccatgcttt gggtggcgtc ctacaacagg accgcgcccg cgctcgacgg cattccattt
      661 ttctattggt atcagctggg ctggatcctt gctggcgccg ccatcgtgct tttggtctat
      721 tggatcgaga gcaaagtcac gcgcgtgacc gggcgaaccg aaccggaagc agagccgagc
      781 ggcgtgccgg gggacattct ttgaacctcg atctcaccgc cacgatcgtc ttcgtcgggc
      841 tgttcctgtt cgtcacctgg ctcggcttcg ttgcggcgcg ttggcgcaag gccgatctca
      901 ctctcctcca cgaatggggc ctcggcggcc gccgcttcgg caccacggtc acctggttcc
      961 tgctcggcgg cgacctctac acggcctaca ctttcatcgc ggtcccggcg ctcgtctacg
     1021 gcaagggggc gatgggcttc ttcgccgttc cctacaccat cctcatttat ccgatcctct
     1081 atatcgtctt tccgcgcctg tgggagatca cccgcgcgcg cgggcacgtc accgcggccg
     1141 acttcgtccg cgatcgcttc ggcagcaggc tgctcgcgct cgccttcgcg ctgacgggca
     1201 ttgtcgccac gatgccctat atcgcgcttc agatcgtcgg catggaggtg gtgatcggcg
     1261 cgctcggctt tccggccgga ggctttgtcg gtcatctgcc gctgatcata tccttcatca
     1321 tcctcgccgc cttcacctat accagcggcc tccgggcgcc ggccttgatc gccatcgtca
     1381 aggacatgct gatctttgtg acggtgttcg ccgcgctcgt gatcatcccg gccgagctcg
     1441 gcggctacgg agcgatcttc aggagcgtgc cgcaagagaa gctgatcctc ccgccaccgt
     1501 ccggcgacag cctcggcgct tacagcgcct atgccacgct cgcgctcggc tcggccttcg
     1561 cgctgttcct ctatcctcac gccatgaccg ggattttgag ctcctcgagc ggcaacgtca
     1621 tcaggcggaa cgcggcgttc cttgccgcct attccttcat tctagggttg atcgcgcttc
     1681 tcggctacat ggcgatcgcc gcgggcgtgg acacaaatcc tgcctatcag gcgggctttg
     1741 cccaatatgg gccgaacttc gccgtgcccg cgctgttcct tgcgtcgttt ccctcctggt
     1801 tcgtcggcgt cgccttcgcc gccatcgcca tcggcgcgct ggtgccggcc gcaatcatgt
     1861 cgatcgcctg cgccaatctc tttacccgca atctctacaa ggagttctac cgtccggatt
     1921 gcagcgaggc cgacgaggcc cgcatggcca agatcgtctc gctcgtggtg aagatcgggg
     1981 cggtggtgtt cattctcgcc attccacagt cctatgccat tcagctccaa ttgcttggag
     2041 ggatttggat cattcagctc ttgccgccga ttctgctcgg gctctatacg cgcggcttca
     2101 atgcgatggc gctgctcgcc ggctgggtcg cgggcaccgt gatcggcacg tatatggcgg
     2161 cgacccaggg cttcacctcc tcggtctatc cgctcgaggc cttcggcctg acggtgccgg
     2221 gttacgccgc cctctattcg gtgctgatca atttcatggt cgctttcgcg cttgcgccga
     2281 tcttcggtcg catcgggcga acgaccgggg ctaccgcgcc ttgaccattg acgcgagctc
     2341 tcgaccgctg cgaatttccc cgataggttc ggagccgcga ttccaacgcc ccgcgacggg
     2401 gaggagattg acccatggca gacgacaacc agggcggcag gacgtttctc gcgatcgtac
     2461 tgggcggcat tgtgctcgcc ctcgtcatcg ctgccttgtt catgtacggg cataagggcg
     2521 agtcggacgt gaaggtcgag aagccgatga ccgaacagcc cgccgagacg cccgcgcccc
     2581 cgcccgcggc cgagatgccg gcagaacctc cgcccgccga gatgccggcc
//
LOCUS       DASUQP010000261         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_401892,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000261 DASUQP010000000
VERSION     DASUQP010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401892"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1485)
                     /gene="fabF"
                     /locus_tag="VFY74_11825"
     CDS             complement(220..1485)
                     /gene="fabF"
                     /locus_tag="VFY74_11825"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5932415.1"
                     /translation="MRRVVITGLGLVSPLGCGYETTWQRLTAGHNAARRITSFEVSDL
                     SCQVACQVPRGDGSNGSFNPTDWMEPKEQRKVDEFIIYAMAAATQALKDADWQPSAYE
                     DQIRTGVLIGSGIGGLQGIEKTSLLFAEKGPRRVSPFFIPGRLINLAAGYVSIEHGLK
                     GPNHAVVTACSTGAHAIGDASRLVQLGDADVMLAGGTESPVCRLAIAGFAACRALSTN
                     FNDRPERASRPYDRDRDGFVMGEGAGVVVLEELEHAKARGARIYAEIIGYGLSGDAFH
                     ITAPAEDGDGAYRCMVAAVKRAGIDPADIDYINAHGTSTPMGDEIELAAVERLFGNAA
                     GKVSMSSTKSAIGHLLGAAGAVEAIFSALAIRDQIAPPTLNLDNPSVVTAIDLVPHQA
                     RRRTIDTVLSNSFGFGGTNASLILRRLNA"
     gene            complement(1588..1827)
                     /locus_tag="VFY74_11830"
     CDS             complement(1588..1827)
                     /locus_tag="VFY74_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5932416.1"
                     /translation="MSDISERVKKIVVEHLGVDADKVTENASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDAIKFLEKNTAA"
     gene            complement(2076..2813)
                     /gene="fabG"
                     /locus_tag="VFY74_11835"
     CDS             complement(2076..2813)
                     /gene="fabG"
                     /locus_tag="VFY74_11835"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX5932417.1"
                     /translation="MFDLTGKGALVTGATGGIGGAVAKVLHAQGANVAVSGTKAERLE
                     ALAAELGARAFVLPCDLRDRGAVAALADTAEKTLGQVDILVNNAGITHDNLFMRMKDE
                     EWDDVIAVNLTSVFVLTRGILRGMMRRRYGRIVNIASISGVFGNPGQGNYAASKAGLV
                     GMTKSLAREVASRGITANCIAPGFIKTPMTDALTPKQVEMIAAAIPAQSFGKPEDVAA
                     AVAFLASTEAAYITGETMHVNGGMVMV"
     gene            complement(2838..>3592)
                     /locus_tag="VFY74_11840"
     CDS             complement(2838..>3592)
                     /locus_tag="VFY74_11840"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5932418.1"
                     /translation="ALMAVSLAAMGVLAEKGLNLSRSVAYVAGHSLGEYSALAASGAL
                     SLADTARLLKTRGRAMQGAVPVGEGAMAALLGADLAQAQALAEAAAEGEVCQAANDNA
                     PGQVVISGTKRAIERAVSLAPKYGARRAVLLPVSAPFHCALMQPAADAMQEALAAVKI
                     DPPAVPLVANVIAASVSDPETIRARLVEQVTGMVRWRETMLYLKDHGVGTVYEVGAGR
                     VLTGIARRFEGMEAKSLGLPEEVEAAAAALAA"
BASE COUNT          598 a         1210 c         1160 g          624 t
ORIGIN      
        1 ttggccggtg gctccggcgg ccgcgtcggc tcgagggcct cggcggggct gcgcggcatc
       61 gcgccgagcg gcgctggcgc gcccggcggg aaccgtccat cgctatcgtt cgccggcgac
      121 ggccggtctc tggtcaagga catggcaagt gagcccctgc gcagacgatg ccaacgactt
      181 gaagtcgaaa tatggcgaaa gggaggttcg gatcgtacgt cacgcgttca agcgcctcag
      241 gatcagggag gcattggtcc cgccgaatcc gaaggagttc gacaacaccg tatcgatggt
      301 ccgccgccgt gcctgatgcg gcacgaggtc gattgcggtc accaccgaag ggttgtcgag
      361 gttgagcgtc ggcggggcga tttgatcgcg aatcgcgagc gccgagaaaa tcgcctcgac
      421 cgcgccggcc gcccccagga gatggccgat ggccgacttg gtcgaagaca tggagacctt
      481 gcccgcggca ttgccgaaca ggcgctcgac ggcggcaagc tcgatctcgt cacccatcgg
      541 cgtcgaggtg ccgtgcgcat tgatatagtc gatatcggcg gggtcgatcc cggcgcgctt
      601 cacggcggcg accatgcaac gataagcccc gtccccgtcc tcggccgggg cggtgatgtg
      661 gaaggcgtcg cccgacagtc cgtatccgat gatctcggcg tagatccggg cgccccgcgc
      721 cttggcgtgc tcgagttcct cgagcacaac gactccggct ccctccccca tgacgaaacc
      781 atcgcgatcg cggtcatagg ggcgcgaggc gcgttccggc cgatcgttga aattggtcga
      841 gagagcgcgg caagccgcga agcccgcgat cgccaagcgg cagaccggcg attcggtccc
      901 gccggcaagc atgacgtcgg cgtcgccgag ctgaacgaga cgcgaggcgt cgccgatggc
      961 gtgcgcaccc gtcgaacagg ccgtgacgac ggcgtggttc ggtcctttga gaccgtgctc
     1021 gatggagaca tagcccgcgg cgagattgat gaggcggccg ggaatgaaga acgggctcac
     1081 ccggcgcggc cccttctcgg cgaagaggag cgacgtcttc tcgatcccct gaaggccgcc
     1141 gatgccggag ccgatcaaga ccccggtgcg gatttgatcc tcataggcgc ttggctgcca
     1201 gtcggcatcc ttgagcgcct gagtggcggc ggccatcgca tagatgatga actcgtcgac
     1261 cttgcgctgc tccttcggct ccatccagtc ggttggattg aagctgccgt ttgagccgtc
     1321 gccgcgcggg acttggcacg ccacctggca ggacaggtcc gagacctcga aactcgtgat
     1381 gcggcgagcg gcgttgtggc cggctgtcag ccgttgccac gtggtctcat aaccgcagcc
     1441 aagcggcgag acgagaccga gacctgtgat gacgacacgt cgcatcgcga ttcgcgtgat
     1501 cctcgcggga aaccactgtt aagccgttcg agggcttttg tctgtcaggc gggacggccg
     1561 cgggcgcgcg tcccgcgcga agtcgcgtca agcggccgtg ttcttctcta ggaacttgat
     1621 ggcgtccccg accgtgagga tcgtctccgc cgcatcgtcg ggaatctcgc agccgaactc
     1681 ttcctcgaag gccatgacga gctcgaccgt gtcgaggctg tcggccccaa ggtcgtcgat
     1741 gaagctcgcg ttctcggtga ccttgtcggc gtcgaccccg agatgctcca ccacgatctt
     1801 ctttacgcgc tccgagatat cgctcatgtc cgcctcaccc gtctctcaac tgtcggtcct
     1861 tacgcttcgc aggtcccccc gagccggcgg ttgacgcacc cccgagcggc aaagtcatgt
     1921 gggttgatcg cggtgggatt gtcgtttgtc aagcccccac ggaacggccg cttgcgtacc
     1981 atactttgca cggccttgcc agaagccgcg acccccgcag gacggcctcg caaatggcct
     2041 cgcgccgttg ctctaaacca ttatatctca aacgcttaca ccatcaccat gccgccattg
     2101 acatgcatcg tctcgcccgt gatgtaggcc gcctcggtgc tggcgaggaa ggcgacagcc
     2161 gcggccacgt cttcgggctt gccgaagctc tgagccggga tcgcggcggc gatcatctcc
     2221 acttgcttgg gcgtgagcgc atcggtcatg ggcgtcttga tgaaccccgg cgcgatgcag
     2281 ttggcggtga tgccccgact cgccacttca cgcgcaagcg acttggtcat gccgacaagc
     2341 cccgccttcg aagcggcata gttgccctgc cccggattgc cgaacacgcc cgagatcgaa
     2401 gcgatattga cgatgcgccc gtagcggcgc cgcatcatgc cccgcaaaat gccccgggtg
     2461 agcacgaaca cggaggtgag gttgaccgcg atcacgtcgt cccactcctc atccttcatg
     2521 cgcatgaaga gattgtcgtg ggtaatgccg gcattgttga cgaggatgtc gacttggccg
     2581 agcgtttttt ccgccgtgtc ggcgagcgcc gccacggctc cccggtcgcg caggtcgcaa
     2641 gggagcacga aggcgcgcgc gccgagttcc gcggcgagcg cttcgaggcg ctcggccttg
     2701 gtgcccgaga cggcgacatt ggcgccttgc gcatgcagca ccttggcgac ggctccgcca
     2761 atcccgccgg tcgcgcccgt gacgagcgcg ccttttcctg tcaggtcgaa catcgaagcc
     2821 tctcctatct ttgcgcctta ggccgccagc gccgccgctg ccgcttcgac ttcctccggc
     2881 aaccccaggc tcttggcctc catcccttcg aaccgccgag caattcccgt cagcacgcga
     2941 ccggcgccga cttcatacac ggtgccgacg ccatggtcct tgagatagag catcgtttcc
     3001 cgccatcgca ccatgccggt gacctgctcg acgaggcgcg cgcgaatggt ttcggggtct
     3061 gagactgacg ccgcgatcac gttggccacg agcggaacag cgggcggatc gatcttcacc
     3121 gccgcgagtg cctcctgcat cgcgtcggcc gcgggctgca tcagagcgca atggaacggg
     3181 gcgctcaccg gaagcaggac cgcacggcgt gccccgtatt ttggcgccaa gctcacggcg
     3241 cgttcgatcg cgcgcttggt gccggagatc acaacctgtc ccggagcatt gtcattggcg
     3301 gcttggcaca cttcgccctc ggccgcggct tcggccagcg cttgcgcttg agcgagatcg
     3361 gcgccgagca aggccgccat cgcgccctcg ccgacgggca cggccccctg catggcgcgg
     3421 ccgcgtgtct tgagcaaccg tgccgtatcg gccaagctga gcgcgccgga cgcggcaagc
     3481 gccgaatact cgccaagcga atgccccgcc acataggcca cgctgcggga aagattgaga
     3541 cccttctcgg caagcacgcc catcgccgcc aatgacaccg ccatcagcgc cg
//
LOCUS       DASUQP010000262         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1901158, whole genome shotgun sequence.
ACCESSION   DASUQP010000262 DASUQP010000000
VERSION     DASUQP010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901158"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..771
                     /locus_tag="VFY74_11845"
     CDS             40..771
                     /locus_tag="VFY74_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEX5932419.1"
                     /translation="MSEAFDIYTLLFLVLAVVIFIRLRNVLGRRTGNERPRYDPYTRP
                     DAKRAGAPEANEPVVALPRSRPTHATVETSGPSTEDIEARLGRHAAKDSPLGQSLTGL
                     IRVDPAFDPNQFLDGAKAAYEMIVMAFAEGDEATLKQLLGAEVYEGFERAIRERESRG
                     EKMESSLIGIDKADIIEAEVKTRTAYITVKFVSELVSVTRDADGEVIEGDPKKVREVT
                     DIWTFARDVTSRNPNWKLVATEAAN"
     gene            861..1907
                     /locus_tag="VFY74_11850"
     CDS             861..1907
                     /locus_tag="VFY74_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX5932420.1"
                     /translation="MSQTSSPLGLEPTRFETLEGWAEDDHAAAFAAFVRSCHADPELP
                     HARAAAALGPGVSSAVARAFFEDRLQPHVIAESSDPGFVTGYYEPEIEGSYARGGPFQ
                     VPVYRRPDDLITVAPDAERARFNDRITGLRQTAAGPVPYYTRHEIWAGALAGHGLELL
                     YLKDEVELFYMQVQGSGLVRLSEGGTVRLSYDGKNGHPYTSIGKLLAERGAIAPGAMS
                     MAAVKAWLRADQARGRALMEENRSYVFFRVLDAEEGRDGPRGAQGVSLTPVRSLAVDT
                     AYHRLGLPIFVTADGLMTEDGHPFRRLMIAQDVGSAIRGPERGDIFWGTGEEAGAIAG
                     STRHRARFHVLMPK"
     gene            1983..2543
                     /locus_tag="VFY74_11855"
     CDS             1983..2543
                     /locus_tag="VFY74_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX5932421.1"
                     /translation="MGRQRDESDEELWERVARSAKPIAKGRAAPPADRPKAPARKAAK
                     EPARAPLPPAAKAAAKPSLAARAELLDRQTVRQLERKRLAVEARLDLHGMRQRDAHAA
                     LRRFLKSAQGKGYRRVLVITGKGAEPDATRSFYEAEDRGVLRQAVPHWLGQPDLASVV
                     ISYAEAPRRLGGEGALYVRLRAPKRQ"
     gene            complement(2576..3880)
                     /gene="hslU"
                     /locus_tag="VFY74_11860"
     CDS             complement(2576..3880)
                     /gene="hslU"
                     /locus_tag="VFY74_11860"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX5932422.1"
                     /translation="MTSFSPREIVSELDRHIIGQRDAKRAVAIALRNRWRRQQLEGDM
                     REEVLPKNILMIGPTGVGKTEISRRLAKLAGAPFIKVEATKFTEVGYVGRDVDQIVRD
                     LLEAGIGLVRDTKRKEVAAKAQLNAEERVLDALVGSGAALSTREAFRKRLRANELDER
                     EIEIQVADTPTMPSFEIPGMPGSQVGMINLSEMLGKTFGTRTKPRKVTVRDSFEILMS
                     EESDKLIDNDMIVQEAVRNVENNGIVFLDEIDKICARSERIGADVSREGVQRDLLPLI
                     EGTTVGTKYGPIKTDHILFIASGAFHLAKPSDLLPELQGRLPIRVELRPLTRDDFRRI
                     LTEPKACLIKQYVALMKTEGVELEFTTDAIEAIADIAVEVNTAVENIGARRLMTVMER
                     ILDNISFDAGDKAGETVVIDAAYVREHIGDLAKNADLSKFIL"
     gene            4089..>4272
                     /locus_tag="VFY74_11865"
     CDS             4089..>4272
                     /locus_tag="VFY74_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932423.1"
                     /translation="MQKIKFTDTSIANLKPRVDRFLVRNVNLAGHYIRIFPSGTKTHY
                     ALARDPSGKQVWHTIGD"
BASE COUNT          750 a         1328 c         1427 g          767 t
ORIGIN      
        1 gccgccgcgg gcacaccaag atgcgggtgt tggcaagata tgagtgaagc tttcgatatc
       61 tatacgcttt tgttcctggt gctcgcggtc gtgattttca ttcggctgcg caatgtgctg
      121 gggcggcgca cgggcaatga gcggccgcgt tacgatccgt acacgagacc cgatgccaag
      181 cgggccggtg cgcctgaagc caatgagccg gttgtggcgc taccgcgcag ccgcccgacg
      241 cacgccacgg tcgaaacgtc cggcccatcg accgaagaca tcgaggcgcg gcttggacgc
      301 cacgccgcca aggacagccc cctcggacag tcgctcacgg ggctgatccg cgtcgatccc
      361 gccttcgacc cgaaccagtt cctcgatggc gccaaggcgg cctacgagat gatcgtcatg
      421 gctttcgccg agggcgacga ggcgacgctg aagcagctcc tcggcgccga ggtctatgaa
      481 ggcttcgagc gcgcgattcg cgaacgcgag agccgcggcg agaagatgga gtcgagtctc
      541 atcggcatcg acaaggccga tatcatcgag gccgaggtca agacccgcac cgcctacatt
      601 acggtgaagt tcgtgagcga actcgtctcc gtcacccgcg acgccgacgg cgaggtgatc
      661 gaaggcgacc ccaagaaggt gcgcgaagtc accgatatct ggacgttcgc gcgcgacgtc
      721 acctcgcgca atcccaactg gaaacttgtc gcgaccgaag ccgccaacta gcgcttggac
      781 gctactatgg aagactcgtc atggccgggc ttgtcccggc catccacgtc tttagatttg
      841 tgcttccgag tcgcgttttg atgtctcaga cgtcatcgcc gctgggcttg gaaccgacgc
      901 gtttcgagac gctcgaaggc tgggcggaag acgaccatgc cgccgccttc gcggccttcg
      961 tgcggtcatg ccatgccgat cccgaattgc cgcacgctcg tgccgccgca gcactcggtc
     1021 ccggggtgag cagcgccgtg gcgcgcgcgt tcttcgagga tcggcttcag ccgcatgtta
     1081 tcgcggaaag ctccgacccc ggctttgtca ccggctatta cgagcctgaa atcgaagggt
     1141 cgtacgcccg cggcggcccg ttccaggtgc ccgtgtatcg ccggcccgac gacctgatca
     1201 cggtcgcgcc cgatgcggag cgggcgcgct tcaacgaccg catcaccgga ttgcggcaga
     1261 cggccgcggg cccggtgccc tactacaccc gtcacgagat ttgggcaggc gcgttggcgg
     1321 ggcatggact cgagctgctc tatctcaagg acgaggtgga gctcttctac atgcaggtgc
     1381 agggatcggg cctggtgcgc ctcagcgaag ggggcacggt caggctctct tacgacggca
     1441 agaacgggca tccctatacc tcgatcggaa agctgctcgc cgagcgcggc gcgattgcgc
     1501 cgggcgccat gtcgatggcg gcggtgaagg cctggcttag agccgatcag gctcgcggac
     1561 gcgcgctcat ggaggagaac cggtcctatg tcttcttccg cgtcctggac gcggaagagg
     1621 ggcgcgacgg tccgcgcgga gcacaaggcg tttcgctcac gccggtgcgg agcctcgccg
     1681 tcgataccgc gtatcaccgg ctcggtctgc cgatcttcgt gacggcggat gggctcatga
     1741 ccgaggatgg acacccattc cggcggctga tgatcgcgca ggacgtcggc tcggcgatca
     1801 ggggtccgga gcgcggcgac atcttctggg ggacgggcga ggaagcggga gcgatcgccg
     1861 gaagcacacg ccaccgcgcg cgatttcacg ttctcatgcc gaaatagaca gtaaccgctg
     1921 tggtgtatga agctacgagc gatggggtta ctggattccc gctttcgagg aatgacaggg
     1981 tcatgggcag gcagagagac gagagcgacg aagagctgtg ggaacgcgtg gcccgcagcg
     2041 ccaagccgat tgccaagggg cgcgccgccc cgcccgccga tcggccgaag gcgcccgcgc
     2101 gcaaggcggc caaggaaccg gcgcgggcgc cgcttccgcc cgcggcaaag gccgcggcaa
     2161 agccttctct cgccgcgcgc gccgagctct tggaccggca aaccgtgcga cagctcgagc
     2221 ggaagcgact tgcggtcgag gcgcggctcg atctccacgg catgcggcag agggacgcgc
     2281 acgcggcttt gcggcgattt ctcaagtcgg cgcaagggaa aggctatcgc cgtgtcctgg
     2341 tcatcacggg caagggcgcc gagcccgacg cgacgaggag tttctacgag gcggaggacc
     2401 gcggggtgct gcgccaggcc gtgccgcact ggcttggcca gcctgacctc gcatccgtgg
     2461 tgatcagcta cgctgaggcg ccccgccgcc tcgggggcga aggcgctctc tatgtgcggc
     2521 tcagggcgcc gaaacgccaa taacggtcgc gccgccagat tttggctgaa ggccgctaca
     2581 aaatgaattt cgacaggtcg gcgttcttgg cgaggtcgcc gatatgctcg cgcacgtaag
     2641 cggcgtcgat gacgaccgtc tcgccggcct tgtcgccggc gtcgaacgag atgttgtcga
     2701 ggatgcgctc catcaccgtc atcaggcggc gggcaccgat attctcgacg gcggtgttca
     2761 cctcgaccgc gatgtcggcg atggcctcga tcgcgtcggt ggtgaattcg agctcgaccc
     2821 cttcggtctt catcagcgcc acgtattgct tgatgagaca cgccttcggc tcggtgagaa
     2881 tgcggcggaa gtcgtcgcgg gtcagcgggc gaagctcgac gcggatggga agccggccct
     2941 ggagttcggg caggagatcc gagggcttgg cgaggtggaa cgcgcccgaa gcgatgaaca
     3001 gaatatggtc ggtcttgatc ggcccgtatt tggtgccgac ggtcgtgccc tcgatcagcg
     3061 gcaggagatc gcgctgaacg ccttcgcggc tgacatcggc tccgatgcgc tccgagcgcg
     3121 cgcagatctt gtcgatctcg tcgagaaaga cgatgccgtt attctcgacg ttcctgaccg
     3181 cttcctgcac gatcatgtcg ttgtcgatga gcttgtccga ttcctcgctc atgagaatct
     3241 cgaacgagtc gcgcaccgtg accttgcgcg gcttggtgcg cgtgccgaac gttttgccga
     3301 gcatctcgga gaggttgatc atgccgactt ggctccccgg catgccgggg atttcgaagg
     3361 acggcatggt cggcgtatcg gcgacctgga tttcgatctc gcgctcgtcg agctcgtttg
     3421 cgcgcaggcg cttgcggaag gcttcgcggg tcgagagagc ggcgccggag ccgaccagcg
     3481 cgtcgagcac gcgctcttcg gcgttgagtt gggccttcgc cgccacctcc ttgcgtttcg
     3541 tgtcgcgcac caggccgatg cccgcctcga gcaagtcgcg cacgatctgg tcgacgtcgc
     3601 ggccgacata accgacctcg gtgaacttgg tggcctcgac cttgatgaac ggcgcgccag
     3661 cgagtttggc gagacgccgc gagatctccg tcttgccgac gccggtcgga ccgatcatca
     3721 ggatgttctt gggcagcacc tcctcgcgca tgtcgccttc gagctgctgc cgccgccagc
     3781 ggttgcggag cgcgatggcg acggcgcgct tggcgtcgcg ctggccgatg atgtgacggt
     3841 cgagctccga gacgatctcg cggggggaaa agctggtcat cgctgcctac gcgttacttg
     3901 gttgcatcga gggtttcgac cacgacctcg tcattggtat tgacgtacag gccgctaaca
     3961 ctgttgaaaa cgcactaccg tggatgtcct tgtaaaccgc caaatttgag cgtatgtaag
     4021 cccaatgata gcccgcatta gtgggggatt ttgttagccc tacgaatgcc cggctaacgg
     4081 agggcacagt gcagaagatc aaattcaccg atacctcgat tgccaatctg aagcctcgag
     4141 tagatcgctt tctggtccgc aacgtcaacc tcgccgggca ctatataagg atctttccga
     4201 gcgggaccaa aacccattac gcccttgcgc gcgatccgag tggcaagcaa gtctggcaca
     4261 ccatcggcga tg
//
LOCUS       DASUQP010000263        15536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_812359,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000263 DASUQP010000000
VERSION     DASUQP010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812359"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VFY74_11870"
     CDS             <1..959
                     /locus_tag="VFY74_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX5932424.1"
                     /translation="KAAYARAANRLNLVLERLEQPLPHTSEIDAGAAHPEPKAATAAQ
                     AYMAKVMRAKDYIAAGDIFQVVLSQSFEAPFSLPSFALYRALRRVNPSPFLYYLDFGG
                     FAVVGSSPEILVRVRDGIVTIRPIAGTRPRGATPEEDKALAKELLGDAKERAEHLMLL
                     DLGRNDLGRVAEIGSVRVTESFFLEYYSQVMHIVSNVEGRLASGFDAVDALMAGFPAG
                     TVSGAPKVRAMEIIDELEDEARGPYAGCVGYFSAGGEMDSCIVLRTSVVKDGTMYVRA
                     GAGIVADSVPEFEQAECVSKARALFRAADEAVRFAARAKRGQ"
     gene            972..1490
                     /locus_tag="VFY74_11875"
     CDS             972..1490
                     /locus_tag="VFY74_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5932425.1"
                     /translation="MLTLALLRHAKSSWSNPTTVDRERPLAARGLADAPLMGKAMADR
                     GIDPELVLCSSARRTRDTLSLVLPELRVEPKVVYEDALYHASAEQMLDILHGLQPGAN
                     RVMLVGHNPEIHAFALDLVGSGPKDFRDRLRAKYPTAALAVINFESGLWKSVAVNSGA
                     LSLFLTPKDLRA"
     gene            1554..2174
                     /locus_tag="VFY74_11880"
     CDS             1554..2174
                     /locus_tag="VFY74_11880"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5932426.1"
                     /translation="MLTLIDNYDSFTYNLVHFLGELGASTRVYRNDKITVDEVLAETP
                     EAIVLSPGPCDPDRAGICLELIARAGPVLPILGVCLGHQAIGQVYGGRVVRAPSLMHG
                     KLSKVHHTGKGVFAGVPQDFSATRYHSLVVERASLPDTLEITAETGDGVIMGLQHKRY
                     PVHGVQFHPESIASEHGHQLLANFLDLARVERRARGKVPRASAGVL"
     gene            2191..3219
                     /gene="trpD"
                     /locus_tag="VFY74_11885"
     CDS             2191..3219
                     /gene="trpD"
                     /locus_tag="VFY74_11885"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5932427.1"
                     /translation="MSELADLRPILRGVADGETLTEKEAAEAFSLIMSGAATEAQIGA
                     LLLGLRVRGETVEEIAGAARALRARAVKVSAPEGAIDTCGTGGDAKGTHNISTCAAFV
                     VAGAGVPVAKHGNRSISSRSGSADVLAALGVNIECGAEAISRSIASCGLGFMFAPAHH
                     AAMRHVAKVRTELGTRTIFNLLGPLANPAGVKYQVVGVFGEAWVEPIARVLGLLGTER
                     AWVVHGADGLDELTTTDISHVAVLDGGNVSTFKVSAKNAGLPEARPEDLIGGDAAANA
                     GALRAVLEGKPGPLRDIVLFNAAAALLVAGKAKTLREGVALAARSIDGGRAKAVLEAL
                     VKLSHDRM"
     gene            3223..4041
                     /gene="trpC"
                     /locus_tag="VFY74_11890"
     CDS             3223..4041
                     /gene="trpC"
                     /locus_tag="VFY74_11890"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5932428.1"
                     /translation="MAQVLDDILAYKRKEVAAAKAAVSEAALRAKAEAQGPVRPFAAA
                     LRSRLAQGEYALIAEIKKASPSKGLIRADFDPPALARAYQEGGATCLSVLTDTPSFQG
                     LPAHISAARDATMLPVLRKDFMLDPYQVNEARALGADCILIILAAVDDATARRLTEQA
                     RDLGMDIVAEVHDEADLDRALRLDTPLIGINNRDLKTFATTLATSERLTPLVPADRIV
                     IAESGIFTHADLARLARSGVRAFLVGESLMRQKDVAAATRALLTGSLTRAEAAQ"
     gene            4038..4514
                     /gene="moaC"
                     /locus_tag="VFY74_11895"
     CDS             4038..4514
                     /gene="moaC"
                     /locus_tag="VFY74_11895"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5932429.1"
                     /translation="MTARLSHLNEKGEARMVDVSGKEVTSRTARAEGFVAMAAETLEL
                     ILSGRVPKGDVLATARIAGIMAAKRTHELIPLCHSLPLTEVTVEFEPSRDPPGLAVTA
                     SAKVDAKTGVEMEALVAVSIACLTIYDMCKAADRAMSFTGIRLQEKTGGRSGTFRR"
     gene            4520..5725
                     /gene="glp"
                     /locus_tag="VFY74_11900"
     CDS             4520..5725
                     /gene="glp"
                     /locus_tag="VFY74_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5932430.1"
                     /translation="MAPVSVAEALARVTKGLAPLEAERVALEAADGRVLAEDVAALVT
                     QPPFDASAMDGYAVRAADVAALPATLRLVGRSAAGAGFRGKVGAGEAVRIFTGAPVPE
                     GADLIVIQENAEEKSGAVVIKEAAGGPYIRRRGQDFTEGEVLLTAGTRLGPRELMLAA
                     AMNPRDLPVRRKPRVAILATGNEVVAPGSELAADQIVSSVPYGLAALIARHGGEAIPL
                     GIAKDEPESIITLARAGSAADILVTIGGASVGEYDLVATALKSEGLELDFWKIAMRPG
                     KPLLFGRLGDQRVLGVPGNPVSALICAHVFLVPMLGKLLGLAEETRPEPEAVLGEALP
                     ANGEREHYMRARSEWRDDGVRVVTPLPSQDSSLVAALARADCLIVRAPNAPAVSEGAS
                     VRIVPLDPG"
     gene            5916..6617
                     /gene="lexA"
                     /locus_tag="VFY74_11905"
     CDS             5916..6617
                     /gene="lexA"
                     /locus_tag="VFY74_11905"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5932431.1"
                     /translation="MLTSKQKELLMFIHERLKETGVPPSFDEMKEALDLRSKSGIHRL
                     ITALEERGFVRRLPHRARAMEIVRLPESVPQGLSGRSRGFSPSVIEGSLGRVPQLAEG
                     EALPQTVSVPVMGRIAAGVPIEAIQTRLSTIEVPPELLTKGEHFALEVKGDSMIEAGI
                     LEGDTIIVKKCDDAGNGDIVVALVDEEEATLKRLRRRGDSIALEAANPSYETRIFGPD
                     RVRVQGRLVGLIRRY"
     gene            complement(6629..7219)
                     /locus_tag="VFY74_11910"
     CDS             complement(6629..7219)
                     /locus_tag="VFY74_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932432.1"
                     /translation="MLGLVIAAFGIALAPQTERPDVLIERGGDTAAVRSDAGSLVFPP
                     ATAASYSAEKWLLADGDERDVPNATDDTVFRCDTLGCIGVVKGKTVALIRHPGALEED
                     CRLADIVIAPFTIGKKCRAARVIVDRRMLKAQGAHALFIEGLSIRTETVAAARGQRPW
                     VPNRAIVPAMRRASPDTGGNQDGAVRDPCGEGNPDE"
     gene            complement(7204..8184)
                     /locus_tag="VFY74_11915"
     CDS             complement(7204..8184)
                     /locus_tag="VFY74_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX5932433.1"
                     /translation="MASWSHVDALRAAINARIRAALPGETGAIAVALITGERGGISED
                     VTESMRDSGLTHILSISGLHMAIMAGTVFWLARALFALVPGLALRYPIKKWAAALALA
                     AATFYLALSGAAVPTVRSWIMMSIVLIAVMLDRPALTMRNVALAALAILLVAPESLFD
                     ASFQMSFAAVVALVALYEWLSGLSRMRLRDASPLWGTFHRGTSLLAGAAATTLVAGTA
                     IAPFALYHFHRMTHYGLVANLLAAPLVSVLIMPMALLSLAAMPFGLEFWPLQVMGAGI
                     DLMVATGEWVASWPGAVSILPQISGLALVLIVLGACGSVFGRRAPACSAS"
     gene            complement(8154..8879)
                     /locus_tag="VFY74_11920"
     CDS             complement(8154..8879)
                     /locus_tag="VFY74_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4131 domain-containing protein"
                     /protein_id="HEX5932434.1"
                     /translation="MWVTGEDERDRNGHDRAGRRDGLLSASLAASARALEAERDRWIL
                     WVPVLFAAGVLSYFALADEPAMRVALALSLGALGFSLAIRHARLGLCIGGAALAFASG
                     FAVAKLRTELVRAPVLAQEFRYVTVSGFVEAHELREKGRARLMLRALSVDGLKPEATP
                     YRVRISMPAKYAAEVKIGEAIALRATLQPPPEPIEPGGFDSRGLPGSTASAQRATPRA
                     RYRSWTARLRRLGTWRPGPMSMR"
     gene            9011..10447
                     /gene="gltX"
                     /locus_tag="VFY74_11925"
     CDS             9011..10447
                     /gene="gltX"
                     /locus_tag="VFY74_11925"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5932435.1"
                     /translation="MREKVVTRFAPSPTGYLHIGGARTALFNWLYARAKGGKMLLRIE
                     DTDQARSTEGAILAILDGLRWLELDWDRDPVYQLTRAERHRAIAEQLLAEGKAYRCYA
                     SPEELQAMRERARAEGRAPGYDGTWRHRDPSEAPAGIEPALRFKAPREGETVIEDRVQ
                     GRVVFANKDLDDLVILRSDGTPTYNLSVVVDDHDMGITHVIRGDDHLTNAARQTQIFK
                     ALGWDTPVYAHVPLIHGPDGAKLSKRHGALGVEAYRAMGYLPAALRNYLVRLGWSHGD
                     AEIMSTEEMVRWFDLDAIGRSAARFDFAKLENLNGHYLRAMPDAEIVKRLKALLPQLP
                     DGAALSQRIGEEGWRKLERAMPGLKERARTLVELLDNASYLFATRPLVLDGQASKLLD
                     ESGRETLAAILPQLESVEPWTQAGLEEAVRTFTEAAGLKLGKVAQPVRAALTGRSTSP
                     GIFDVLEVLGREESLARLRDQVHAGSPA"
     gene            10577..11893
                     /gene="gltA"
                     /locus_tag="VFY74_11930"
     CDS             10577..11893
                     /gene="gltA"
                     /locus_tag="VFY74_11930"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX5932436.1"
                     /translation="MSVAENLRAPGAPTATVLIDGVRYDLPVLKGTLGPDVIDINAFY
                     QETGCFTYDPGYTSTASCESKITFIDGDKGVLLYRGYPIDQLAEQGTFLETAYLLLYG
                     ELPTAAQFKEFKERITRHTMVHEQMTRFYTGFRRDAHPMAVMVGVVGALSAFYHDSID
                     IKDPSQREMASIRMIAKMPTIAAMAYKYSIGQPFIYPKNELDYSSNFLHMCFAVPCEE
                     YQVNPVLARAMRRIFILHADHEQNASTSTVRLAGSSGANPFACISAGIACLWGPAHGG
                     ANEAALTMLQEIGTVDRIPEFIQRAKAKDDPFRLMGFGHRVYKNYDPRAKIMQKTAHE
                     VLEAVGHRNDPILKVALELERIALSDDFFIEKKLYPNIDFYSGITLKALGFPTDMFTV
                     LFAIARTVGWIAQWKEMLTDPMQRIGRPRQLYTGATERDYVPMDRR"
     gene            complement(11890..12759)
                     /gene="lpxI"
                     /locus_tag="VFY74_11935"
     CDS             complement(11890..12759)
                     /gene="lpxI"
                     /locus_tag="VFY74_11935"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX5932437.1"
                     /translation="MPEAVKIEGPLGIIAGRGPLPRAVAEAAASRGLSLHIVGIRGEA
                     RDEIERFPHTWVKWGEIGKMFRALDQNGCGDIVIIGGVNRPDFSNVRLDLGAIKTLPF
                     ILSLGKGGDDHVLSRVVRFFEQKGYRVHGADAVAPELLAEEGVLGGKAPNAEDKADID
                     LAFRVVGALGRLDVGQAAVVAKGYVLAVEAAEGTDAMLARCAELRAAGRTGKRGRAGV
                     LVKVPKFGQEERVDLPTIGPDTVTKAAGAGLAGIAVAAGRVLMADRDATIATADRNGL
                     FLLGVSLAGRDGA"
     gene            complement(12777..13583)
                     /gene="lpxA"
                     /locus_tag="VFY74_11940"
     CDS             complement(12777..13583)
                     /gene="lpxA"
                     /locus_tag="VFY74_11940"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX5932438.1"
                     /translation="MVNIHATAVVDPSAKLGTGVTIGPFCVIGPDVELGDGAVVHSHA
                     VIGGRTTIGEGCQVYPFASIGQRPQDVKYRGEPSTLTIGPNTTIREHVTVNPGTEGGH
                     MMTKVGANCLLMIGVHVAHDCALGDNVTLVNAATLGGHVSIGDGAIIGGLSAVHQFVR
                     IGAYAFVGGMSGITADVIPFGMAIGNRANLVGLNVVGLKRKGFPREQIHELRQAYRML
                     FSSEGTLKERLEDVESMFSANPLAKQIIDFIKKESDRSFCVPNSAAPPAR"
     gene            complement(13596..14063)
                     /gene="fabZ"
                     /locus_tag="VFY74_11945"
     CDS             complement(13596..14063)
                     /gene="fabZ"
                     /locus_tag="VFY74_11945"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5932439.1"
                     /translation="MNESGVKSKLGKAEITDILKLLPHRYPFLLVDRIVDMDGDRSAV
                     GIKNVTVNEPYFQGHFPGNPVMPGVLLIEGMAQTAGALCMRHLEEFKEPPLVYFMAID
                     KARFRRPVVPGDTVHYHVQKMRNRGRVWRFRAQAHVDGTLVAEAEVSAVIVET"
     gene            complement(14097..15173)
                     /gene="lpxD"
                     /locus_tag="VFY74_11950"
     CDS             complement(14097..15173)
                     /gene="lpxD"
                     /locus_tag="VFY74_11950"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX5932440.1"
                     /translation="MEHPGFFERAGPFALGTVAEAAHAKPAGGTDLRTAIKDVRPLDN
                     AGPGDLSFLDNPKYLSLFARTAASACLVAPKFADQAPAGTACLITPEPYRAFARALLL
                     FYPDAVKPKAALTDGGDGPASVHPSAALEPGTIVEPGAVIAPEARIGRGTTIAAGSVI
                     GFRVHIGRDCYIGPNASITHALVGNQVTIHAGVAIGQDGFGFAMGKNGHLKVPQIGRV
                     IIQDGVEIGANTTIDRGALRDTVIGEGTKIDNLVQIGHNVVIGRHCVIVAQTGISGSA
                     ELGDFVVLGGQVGVVGHVKIGAGAQIAASSNVRGDVPPGVRWGGTPAKPVRLWFRELT
                     LLQKLAERKDLKLDHDEGGSETGA"
     gene            complement(15246..>15536)
                     /locus_tag="VFY74_11955"
     CDS             complement(15246..>15536)
                     /locus_tag="VFY74_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX5932441.1"
                     /translation="TAEVRFPIPYIPESLGMQAAVFVDAGSLWQPSGLALDAVADEGS
                     FILDSTEVRLSTGFSLIWQSPLGPLRADFAEALLKADFDKTEFFRFGASTNF"
BASE COUNT         2722 a         5178 c         5045 g         2591 t
ORIGIN      
        1 ccaaggcggc ctatgcgcgc gcagccaacc ggctgaacct cgtattggag cggctcgagc
       61 agccgctgcc gcatacgagc gagatcgacg cgggggcggc gcatccggag cccaaggccg
      121 ccaccgcggc ccaggcctat atggcgaagg tgatgcgcgc gaaggactat attgccgcgg
      181 gcgacatctt tcaggtggtg ctgagccaga gcttcgaggc gcccttcagc ctcccctctt
      241 ttgcgcttta ccgcgcgctc cggcgcgtca atccctctcc cttcctctac tacctcgatt
      301 ttggcggctt cgccgtcgtg ggttcgagcc ccgagattct cgtcagggtg cgcgacggta
      361 ttgtcaccat caggccgatt gccggcacgc ggccaagagg ggcaacgccg gaggaggaca
      421 aggcgcttgc caaagagctg ctcggcgacg ccaaggagcg cgccgagcat ctgatgctgc
      481 tcgacctcgg ccgcaacgat ttggggcgcg tggcggagat cggcagcgtg cgcgtgaccg
      541 aatcgttctt cctcgagtat tacagccagg tgatgcacat cgtgtcgaat gtcgagggga
      601 gacttgcttc cggcttcgac gccgtcgatg ccctgatggc gggcttcccc gccggcacgg
      661 tctcgggcgc gccgaaagtg cgcgccatgg aaatcatcga cgaacttgag gacgaggcac
      721 gcggtcccta tgcgggctgc gtcggttatt tctcggccgg cggcgagatg gacagctgca
      781 tcgttcttag gacctcggtg gtgaaggatg ggacgatgta tgtccgggcc ggcgccggca
      841 tcgtcgccga ttcggtgccc gagttcgaac aggccgaatg cgtgagcaaa gcgcgcgcgc
      901 tgttcagggc cgccgacgaa gccgtgcgtt tcgccgcccg cgccaagcgt ggccagtaag
      961 cggctcggct catgctcacg cttgctcttc tccgtcacgc caaatcaagt tggagcaacc
     1021 ccacgacggt cgatcgcgag cggccgcttg cggcgcgggg gctcgccgac gcgccgctca
     1081 tgggcaaggc catggccgat cgcggcatcg atccggagct cgttctctgc tcgtcggcgc
     1141 ggcgcacccg cgacacgctc agccttgttc ttccggaact ccgcgtcgag cccaaggtcg
     1201 tctacgaaga cgcgctttat cacgcgagcg ccgagcaaat gctcgacatc ctgcatggcc
     1261 ttcagccggg cgcgaaccgc gtaatgctcg tcgggcacaa tcccgagatc catgccttcg
     1321 cgctcgacct cgtgggctcc gggcccaaag atttccggga tcgcctgaga gcgaaatatc
     1381 cgaccgcggc cctggccgtg atcaatttcg aaagcggttt gtggaagagc gttgcggtca
     1441 atagcggggc gttaagcctg ttcctgaccc cgaaggattt gcgcgcctag cgctcgcccg
     1501 ccgcgccttg ccgcgtcttg acgggacgta ggcgccgagc gtaacaccac ccgatgctca
     1561 cgctcatcga taattacgac agcttcacct acaacctcgt ccattttctc ggcgaattgg
     1621 gcgcctcgac gcgcgtctat cgcaacgaca agatcaccgt agacgaggtc ttggcggaaa
     1681 cgcccgaggc gatcgttcta tcgcccggac catgcgaccc ggatcgcgcc ggaatctgcc
     1741 tggaactcat cgcgcgcgcg gggcccgtgc tgccgatcct cggcgtctgt ctcggccacc
     1801 aggccatcgg ccaggtctat ggcggccgcg tggtgcgcgc ccccagcctg atgcacggca
     1861 aattgtcgaa ggttcatcac accggaaaag gcgtcttcgc cggcgtcccg caggacttct
     1921 cggccacgcg gtaccattcg ctggtggtcg agcgcgcaag cctgcccgac acgctcgaga
     1981 tcacggcgga gacgggcgac ggcgtcatca tgggacttca gcataagcgc tatcccgtgc
     2041 acggggtcca gttccacccc gaaagcatcg cctctgagca cggccatcag cttcttgcca
     2101 attttctcga cctcgcccgc gtcgaacggc gcgcgcgcgg aaaagtgcca cgagcatcag
     2161 ccggtgtcct ctaaccgctc gaccgcgagc atgtcggagc ttgcggacct tcgccccatt
     2221 ctccgcggcg tggcggacgg cgagacgctg acggagaaag aagcggccga ggccttttcg
     2281 ctcatcatgt cgggcgcggc gaccgaggcg caaatcggcg cgcttctgct gggccttcgc
     2341 gttcgcggcg agacggtgga agaaatcgcc ggcgccgctc gcgcgctgcg cgcacgcgcc
     2401 gtgaaggtca gcgcgccgga aggcgccatc gatacgtgcg gcaccggcgg cgacgccaag
     2461 ggcacgcaca atatctcgac gtgtgccgcc ttcgtggttg cgggtgccgg cgtgccggtc
     2521 gccaagcacg gcaaccggtc gatctcttcg cgctccggct cggccgatgt gctggccgcg
     2581 ctcggcgtca atatcgaatg cggggccgag gcgatttcgc gctcgatcgc ctcatgcggg
     2641 ctcggcttca tgttcgcgcc ggcgcatcat gccgccatgc gccatgtcgc caaggtgcgg
     2701 accgagctcg gcacgcgcac catcttcaac ctgcttggcc cgctcgccaa tcccgccgga
     2761 gtaaaatatc aggtcgtggg cgtcttcggc gaggcgtggg tcgaacccat cgcgcgcgtc
     2821 ctcggattgc tggggaccga gcgggcctgg gtcgtgcacg gcgccgacgg cctcgacgag
     2881 ctgacgacga ccgacatcag ccatgtggcg gtgctggatg gcggcaacgt ctcgaccttc
     2941 aaggtctcgg cgaagaacgc cgggctgccc gaggccaggc ccgaggatct gatcggcggc
     3001 gatgcggccg cgaacgcggg cgcgcttcgc gccgtgctcg aagggaaacc cggtccgttg
     3061 cgggacattg ttcttttcaa cgcggccgcg gcgttgctgg tcgcaggcaa ggccaagacc
     3121 ttgcgcgagg gcgtggcgct cgccgctcga tccatcgatg gcggcagagc gaaagccgtg
     3181 ctcgaagcgc tcgttaagct cagccatgat agaatgtgag ccatggcaca ggtgctcgac
     3241 gacatcctcg cctataagcg caaggaggtg gcagccgcca aggcggcggt gagcgaagcc
     3301 gccttgcgcg ccaaagccga ggcccaaggc ccggtgcggc ccttcgcggc cgcgctgcgg
     3361 tcgcggcttg cccaaggcga atatgccctc attgccgaga tcaaaaaggc aagcccctcg
     3421 aagggactga tccgcgccga tttcgatccg ccagcgctcg cccgcgccta tcaggaaggc
     3481 ggcgcaacct gcctttcggt tttgaccgac acgccgtcct ttcaggggct ccccgcccat
     3541 atctccgcgg cgcgcgacgc gacgatgctc ccggtcctgc gcaaggactt tatgctcgat
     3601 ccctatcagg tgaatgaggc ccgcgcgctc ggcgccgatt gcatcctcat cattctcgct
     3661 gcggtcgacg atgcgaccgc acggcgcctg accgagcaag cgcgcgatct cggcatggac
     3721 attgtcgccg aggtgcatga cgaggccgat ctcgaccgcg ccctccgcct cgacacgccg
     3781 ctcatcggca tcaacaaccg cgacctcaag actttcgcaa cgacgcttgc cacaagcgag
     3841 cgtctcacgc cgctcgtgcc tgccgatcgc atcgtcattg ccgagagcgg catcttcacc
     3901 catgccgatc ttgcgcggct tgcccgctct ggcgtgcgtg ccttcctcgt cggcgaaagc
     3961 ctgatgcggc agaaggacgt ggcggcggca acccgcgcgc tcctgaccgg gtccttgacg
     4021 cgagcggagg cggcccaatg acggcgcgtc tcagtcactt gaacgaaaag ggcgaggccc
     4081 gcatggtcga cgtgtcgggc aaagaggtca cgtcccgtac cgcccgcgcc gagggtttcg
     4141 tcgccatggc agcggagacg ctcgaactga tcctttcggg gcgcgtgccc aagggggacg
     4201 tgctcgcgac cgcccgcatc gccgggatca tggccgcaaa gcggacgcat gagctgatcc
     4261 ctctttgcca ttcgctgccg ctcacggagg tgacggtcga attcgagccg tcgcgcgatc
     4321 cgcccggcct cgccgtaacg gcttcggcca aggtcgacgc aaagaccgga gtcgagatgg
     4381 aggcgctggt cgccgtgtcg attgcctgtc tcaccatcta cgacatgtgc aaggcggccg
     4441 accgggcgat gtccttcacc ggcatccgtc ttcaggaaaa gacgggcggg cgctcgggca
     4501 cgttccgccg ctgaccgcaa tggctcctgt ctccgttgca gaggctcttg cccgcgtgac
     4561 caaggggctc gcgccgctcg aggccgagcg cgtggcgctc gaggcggctg acggccgcgt
     4621 gcttgccgag gatgtcgctg cactggtgac ccagccgcct ttcgatgcct cggcgatgga
     4681 cggctacgcg gtccgcgccg ccgacgtggc ggcgctcccg gcaacgctgc gcctggtcgg
     4741 acggtctgcg gcgggcgcgg gcttccgcgg caaagtggga gccggcgaag ccgtgcgcat
     4801 cttcaccggc gcgccggtcc ccgaaggggc cgatctcatc gtcattcagg aaaacgccga
     4861 agagaaatcg ggggctgtgg tcatcaagga ggccgcaggc ggtccttaca tccgccggcg
     4921 ggggcaggat ttcaccgagg gcgaggtgct cttgacggcg ggcacgagac ttgggccgcg
     4981 cgagctgatg cttgccgccg ccatgaaccc gcgtgatctg cccgttcggc gcaagccgag
     5041 ggtggcgatc ctcgccaccg gcaacgaggt cgtggcgcct ggctccgagc tcgcggccga
     5101 tcagatcgtg tcctccgtgc cttacgggct tgcggcgctg attgcgcgcc acggcggcga
     5161 ggcgattccc ctcggcatcg ccaaggacga gccggagagc atcatcacgc tcgcccgcgc
     5221 cggaagcgcc gccgacatcc tcgtcacgat cggcggggcc tctgtcggcg agtacgatct
     5281 ggtggcgaca gcactcaagt ccgaggggct cgagctcgat ttctggaaga tcgccatgcg
     5341 cccgggcaag cccctgctct tcggccggtt gggagatcag cgcgttctcg gcgtgccggg
     5401 caacccggtc tcggcgctga tctgcgccca cgtgtttctc gtccccatgc tcggcaagtt
     5461 gctggggctt gccgaagaga cgcgtcctga gcccgaggca gtcctcggcg aggcgcttcc
     5521 cgccaacggt gagcgcgagc attacatgcg ggcgcggtcg gaatggcgcg acgacggggt
     5581 gcgggtcgtc acccccctgc cctcgcagga ctcctctctg gtggcggcgc tcgcgcgtgc
     5641 cgactgcctc atcgtgcgcg ctccaaacgc gcctgcggtt tcagaaggcg cttccgtcag
     5701 gatcgtgccg ctcgaccccg gttaaggcgt cggaaacccg aatcgcgcga cctgtggcgg
     5761 gtcggctgcc ctggccaccc cccccccgca tcttgggggt taaacccttg cggaacacaa
     5821 acagaacaga taaacttgtt catgatttgt acaggcgagg cgaatcgcta gggcggcgcc
     5881 gatcaggggc aagcccaccg ctcgaagggg accagatgct cacatcgaag caaaaagagc
     5941 ttctcatgtt cattcatgag cggttgaagg aaaccggcgt gccgccctcg ttcgacgaga
     6001 tgaaggaggc actcgacctc aggtcgaaat caggcatcca ccgtctcatc acggcgctcg
     6061 aggagcgggg cttcgtccgc cgcctgcccc accgtgcacg cgccatggag atcgtgcgct
     6121 tgccggaatc cgtgccccag gggctttccg ggcggagccg aggcttctcg ccgagcgtaa
     6181 tcgagggaag ccttgggcgc gttccccagc tggccgaagg cgaggccctg ccgcagacag
     6241 tttcggttcc ggtgatgggc cgtatcgccg ccggcgtccc catcgaagcc atccaaacgc
     6301 ggctcagcac catcgaagtg ccgcccgagc ttctcaccaa aggcgagcat ttcgcgctcg
     6361 aggtcaaagg cgattccatg atcgaggccg gcatcctcga aggcgatacc atcatcgtca
     6421 agaaatgcga cgacgccggc aacggggaca tcgtcgtggc gctcgtcgac gaggaggaag
     6481 cgacgctcaa gcgcttgcgc cgccgcggcg actcgatcgc gctcgaagca gccaacccgt
     6541 cctatgaaac gcgcatcttc ggccccgacc gcgtgcgcgt gcagggacgc ctcgtcgggc
     6601 tgatccgccg ctactagagc attagcattc attcgtcagg gttgccttcc ccacacgggt
     6661 cgcgcaccgc gccgtcttga ttgccgccgg tatcgggcga cgctctccgc atcgcgggga
     6721 caatggcgcg gttgggcacc cagggcctct gtccgcgcgc cgctgcaacc gtctccgtcc
     6781 tgatcgatag tccctcgata aagagcgcat gcgccccttg cgctttcagc atgcggcgat
     6841 cgacgatgac gcgcgcggcg cggcatttct tgccgatggt gaagggcgca atgacgatgt
     6901 cggcaagccg gcagtcctcc tccagcgcgc cgggatggcg gataagcgcc accgtcttcc
     6961 ccttgaccac gccgatgcag cccaaagtgt cgcagcggaa gacggtgtca tccgtcgcgt
     7021 tggggacgtc gcgttcgtca ccgtcggcaa gcagccattt ttccgcgctg tagctcgccg
     7081 ccgtcgccgg gggaaatacg aggctgccgg cgtccgacct gaccgccgcg gtatcgccac
     7141 cccgctcgat gagcacgtcg ggccgctcgg tctgcggcgc gagcgcgatg ccgaaagctg
     7201 cgatcacgag gccgagcatg cgggcgcgcg tctgccaaag acagagccac aagccccgag
     7261 cacgatgagg acgagcgcga ggcccgaaat ctgcggcaag atcgagactg cgccgggcca
     7321 ggacgccacc cattcgcccg ttgcgaccat cagatcgatg ccggctccca tcacttgaag
     7381 cggccagaac tccagcccga atggcatggc ggcaaggctc agcagcgcca tcggcatgat
     7441 caggacgctc accagggggg cggctagcag attggcgacg agcccgtaat gcgtcatgcg
     7501 gtggaagtga tagagcgcaa agggcgcgat cgcggtgccg gcaacgagcg tcgtggcggc
     7561 cgcgcccgcg aggaggctcg tgccccggtg gaaggttccc cagaggggac ttgcgtcgcg
     7621 caatctcatc cggctcagcc ctgacagcca ttcatagagc gcgacgagcg cgaccacggc
     7681 cgcaaacgac atttggaagc tggcgtcgaa cagcgattca ggagcgacga ggaggatggc
     7741 aagagcggca agcgcgacgt tgcgcatggt cagcgcaggc cggtcgagca tgaccgcgat
     7801 caagacgatg ctcatcatga tccaggagcg aaccgtgggc acggcggcgc cagacaacgc
     7861 gagatagaag gtggccgcgg caagggcgag cgcggcagcc catttcttga tcggataacg
     7921 gagcgcaagt cccggcacga gagcgaacag cgcgcgcgcg agccagaaca ccgttcccgc
     7981 catgatcgcc atgtgcaggc cggagatgga gaggatgtgt gtcagcccgg aatcgcgcat
     8041 ggactcggtc acgtcctctg agatgccgcc ccgttcgccg gtgatgagcg caaccgcgat
     8101 cgcgccggtc tcgccgggca aggccgccct gatgcgggcg ttgatcgcag ctctcaacgc
     8161 atcgacatgg gaccaggacg ccaagtccca aggcggcgca ggcgcgccgt ccaggagcga
     8221 taccttgctc gtggcgtagc ccgttgcgcc gaggcggtcg aaccaggcag cccgcgcgaa
     8281 tcgaaaccgc cgggctcgat cggctctggc ggcggctgga gcgtcgcccg caacgcgatc
     8341 gcttcgccga tcttcacctc cgccgcatac tttgcgggca tgctgatgcg gacgcggtag
     8401 ggcgttgcct cgggcttcag gccatccacg gaaagggccc gcagcatgag ccgggccctc
     8461 cccttttcgc gcaattcgtg cgcctcgacg aagcccgaca ccgtgacata gcggaactct
     8521 tgcgcgagca caggggcccg caccaactcg gtccgaagct tggcgacggc gaagcctgag
     8581 gcaaaagcga gcgctgcccc gccgatgcat agcccgagcc gcgcatgcct gatcgcgaga
     8641 ctgaagccga gtgcgccgag cgaaagcgcg agcgccaccc gcatggcagg ctcgtcggca
     8701 agcgcgaaat aggagaggac gccggcggcg aacagcaccg ggacccagag gatccagcgg
     8761 tcccgctccg cttcgagtgc gcgcgccgaa gcggcgaggc tcgcagacag cagcccgtcg
     8821 cggcgtcccg cgcggtcgtg gccgttacgg tcgcgctcgt cttcccccgt gacccacatg
     8881 gccgacgctc agcaccggca gaggcgcctc gcccctgccc ttaccccgca tccaccccta
     8941 tgctacatca cgcccgcttg accccctagc cccgcatcgg aacggccaag aagcgcccga
     9001 aggaacgctt atgcgcgaaa aagtcgtcac gcggttcgcc ccctcgccca ccggctatct
     9061 gcatatcggc ggggcgcgca cggcgctgtt caattggctc tatgcgcgcg ccaagggcgg
     9121 caagatgctg cttcgcatcg aggacaccga tcaggcgcgc tcaacggagg gggcgattct
     9181 cgcgatcctc gacggcttgc gctggctcga gctcgattgg gaccgagacc ctgtctacca
     9241 gcttacgcgc gcggagcgcc atcgcgcgat cgccgagcaa ctgcttgccg agggcaaagc
     9301 ctatcgctgc tacgcgagcc ccgaggagct tcaagccatg cgcgagcgtg ctcgcgccga
     9361 ggggcgcgcg ccaggctatg acggcacctg gcgccatcgc gatccctcgg aagcgcccgc
     9421 aggcatcgag cccgcgctcc gcttcaaggc cccgcgcgaa ggcgagaccg tgatcgagga
     9481 ccgggttcag ggccgcgtcg tctttgccaa caaggacctc gacgatctgg tgatcctgcg
     9541 cagcgatgga accccgacct acaatctctc cgtcgtggtc gacgaccacg acatgggcat
     9601 cactcatgtc atccgcggcg acgaccatct caccaatgcg gcccgccaga ctcagatctt
     9661 caaggcgctt ggatgggaca cgccggtcta cgcccacgtc cctctcatcc atggtcccga
     9721 tggcgccaaa ttgtccaagc gccatggcgc gctcggcgtc gaagcctatc gggccatggg
     9781 ttacctgccg gcggcgctgc gcaattatct cgtgagactc ggctggagcc acggggacgc
     9841 cgagatcatg tcgacggaag agatggtccg ctggttcgat ctcgatgcga tcggccgctc
     9901 ggcggcgcgg ttcgatttcg ccaaactaga gaatctgaac ggccattatc tccgcgccat
     9961 gcccgacgcc gagatcgtga agcggctgaa ggcgcttctt ccccagctcc cggacggcgc
    10021 tgcgctcagc cagcggatcg gcgaggaggg ctggcgcaaa ttggagcgcg ccatgccggg
    10081 gctgaaagag cgtgccagga cccttgtcga gcttctcgac aacgcgtctt acctgttcgc
    10141 gacgcgaccg ctagtcctcg acgggcaggc ctcgaagctc ctcgacgaat cggggcgcga
    10201 gacgctcgcg gctattttgc cgcagctcga atcggtcgag ccgtggacgc aagcgggcct
    10261 tgaggaggcc gtccggacct tcaccgaggc ggccggactc aagctcggca aagtcgccca
    10321 gccggtgcgg gcggccttga cgggacggtc gacttctccg ggtattttcg atgtgctcga
    10381 ggtcctcgga cgcgaggaat cgcttgcccg cctgcgcgat caagtgcatg caggaagccc
    10441 tgcttaatat ctaggcaagc ccggttgttg cgctgcggga caatcggcgc gattatgtgg
    10501 cagtgcaaaa attccttcgg ccgccaacgc gcaaccggcg cgagatagcg gctatgacgg
    10561 tcagaggagc ctttcgatga gcgtggcaga aaatcttcgc gcgcccggcg cacccacggc
    10621 gactgtcctg atcgacggcg tgcggtacga cttgcccgtc ctcaagggca cgctcggtcc
    10681 cgatgtcatc gacatcaacg ccttctacca ggagaccggc tgcttcacct acgacccggg
    10741 ctacacctcg acggcgagtt gcgagtcgaa aatcaccttc atcgacggcg acaagggcgt
    10801 cctgctctat cgcggctatc ccatcgatca gctcgccgag caaggaacct ttcttgagac
    10861 ggcttacctg ctgctctatg gcgagctgcc gacagccgcg cagttcaagg agttcaagga
    10921 gcgcatcacc aggcacacaa tggtgcatga gcagatgacg cggttctaca ccgggttccg
    10981 gcgcgacgca catccgatgg ccgtcatggt gggcgtggtc ggcgcgctct cggccttcta
    11041 tcacgactcg atcgacatca aggatccgag ccagcgcgag atggcgagca tccgcatgat
    11101 cgcaaagatg ccgacgatcg cggccatggc gtacaaatac tcgatcgggc agcccttcat
    11161 ctatcccaag aacgagctcg actactcttc caactttctg cacatgtgct tcgccgtgcc
    11221 gtgcgaggag taccaggtca atccggtgct cgcgcgcgcg atgcggcgca tcttcatcct
    11281 tcatgccgac cacgagcaga acgcctcgac ctccacggtg cgacttgccg gctcatcggg
    11341 cgcaaacccg tttgcgtgca tctcggccgg cattgcctgt ctttgggggc ctgcccatgg
    11401 cggcgccaac gaggctgcgc tgaccatgct gcaggagatc ggcacggttg accgcatccc
    11461 cgagttcatc cagcgcgcca aggccaagga cgatccgttc cgtctcatgg gcttcggcca
    11521 ccgggtctac aagaactacg acccgcgggc gaagatcatg cagaagacgg cccacgaggt
    11581 gctcgaggcc gtcggtcacc gcaacgatcc gatcctcaaa gtcgcgctcg agctcgagcg
    11641 catcgcgttg tcggacgatt ttttcatcga gaagaagctc tatcccaaca tcgatttcta
    11701 ttccgggatc acgctgaaag cgcttggatt cccgacggac atgttcaccg tgctgttcgc
    11761 catcgcgcgc acggtgggct ggattgcgca gtggaaggag atgctcaccg atccgatgca
    11821 gcgcatcggc aggccgcgtc agctctacac gggcgcgacc gagcgggatt acgttccgat
    11881 ggaccggcgc taggcgccat cgcgcccggc caagctcacg ccaagcagaa aaagtccgtt
    11941 gcggtcggcc gtcgcgatcg tggcgtcgcg gtctgccatc agcacgcgtc ccgccgccac
    12001 ggcgatgccg gcaagaccgg cgccggccgc cttcgtgacg gtgtcggggc caatggtggg
    12061 gaggtcgacg cgttcttcct ggccgaactt cggcaccttg acgaggaccc ccgcccggcc
    12121 gcgcttgccc gtccgtcccg cagcgcgcaa ctcggcgcac cgggcgagca tcgcgtcggt
    12181 cccttccgcc gcctcaacgg cgagaacgta acctttggcg acgactgctg cctgcccgac
    12241 atcgagccgc ccgagcgcgc cgacgacgcg gaacgccaaa tcgatatcgg ccttgtcctc
    12301 cgcgtttgga gccttgccgc caaggacccc ctcctcggcc aagagctccg gcgcgacggc
    12361 gtccgcgcca tgaacgcggt accctttctg ctcgaagaag cgcacgaccc gcgacagcac
    12421 atgatcgtcg ccgcccttgc cgaggctgag gatgaagggc aaggtcttga tggcgccgag
    12481 gtcgagccgg acattggaaa agtccggacg gttcaccccg ccgatgatga cgatatcgcc
    12541 gcatccgttt tggtcgaggg cgcggaacat cttgccgatt tcgccccatt tcacccaggt
    12601 atgcggaaac cgttcgatct cgtcgcgcgc ttcgccgcga atgccgacaa tatggagcga
    12661 gagcccccgc gacgccgccg cctcggcgac cgcgcgcgga agcggacccc gcccggcgat
    12721 gattccgagc gggccttcta tcttgactgc ctcgggcacg gctgctgcca gctccgtcag
    12781 cgcgccggcg gtgcggcgct gttcggcacg cagaaggagc gatcggattc tttcttgatg
    12841 aagtcgatga tctgtttggc cagcggattg gccgagaaca tgctctcgac gtcctcgagc
    12901 cgctccttca gcgttccctc gctcgaaaac agcatgcgat aggcttggcg aagctcgtgt
    12961 atctgctcgc gcgggaatcc cttgcgcttc agaccgacga cgttgagccc gacgaggttg
    13021 gcgcgattgc cgatggccat gccaaacggg atgacgtcgg cggtgatgcc ggacatgccg
    13081 ccgacgaagg cgtaggcgcc gatgcgcacg aattggtgga cggcggacag gccgccaata
    13141 atggcgccgt cgccgatcga cacgtggccg ccaagcgtcg cggcgttgac caacgtgaca
    13201 ttgtcgccga gcgcgcagtc atgcgcgaca tggacgccga tcatcagcag gcagttggcg
    13261 ccgaccttgg tcatcatgtg accgccctcc gtgccggggt tgacggtgac atgctcgcgg
    13321 atcgtggtgt ttgggccgat ggtcagcgtg ctcggctcgc cgcgatactt cacgtcctgc
    13381 ggcctctgac cgatggaagc gaaggggtac acctggcagc cttccccgat cgtcgtgcgg
    13441 cccccgatca cggcatgcga atgcacgacg gcgccatcgc cgagttcgac atcgggtccg
    13501 atcacgcaaa agggaccgat cgtgacgccc gtcccaagtt ttgcgcttgg atcgaccaca
    13561 gcggtcgcat ggatgttgac catgctcgcc cgacttcaag tctcgacgat cacggcgctc
    13621 acctcagcct cggcgacgag ggtgccgtcg acatgcgcct gcgcgcgaaa gcgccagacg
    13681 cgcccgcgat tgcgcatttt ttgaacgtgg tagtggacag tatcgccggg caccacgggg
    13741 cggcggaacc gggccttgtc gatggccatg aaatagacaa ggggcggttc cttgaactct
    13801 tctagatggc gcatgcagag ggcgccggcg gtctgcgcca tgccctcgat caggagcacg
    13861 ccgggcatca cgggattgcc ggggaagtgt ccctggaaat aaggctcgtt gacggtcacg
    13921 ttcttgatgc cgacggccga gcgatcgccg tccatgtcga cgatgcggtc gaccaggaga
    13981 aacggataac ggtgcggcag aagcttcagg atgtcggtaa tttccgcctt cccgagcttc
    14041 gatttcactc cgctctcatt catcgacgcc aactccctgg acgggccctg cgcaccctaa
    14101 gcgcccgtct cgcttccgcc ctcgtcgtga tcaagcttca aatcctttcg ttcggcaagc
    14161 ttttgcaaca gcgtaagctc gcggaaccag agacgcaccg gcttggcggg ggtcccaccc
    14221 catctgaccc ccggcggcac gtcgccccgc acattactgc tcgcggcaat ctgcgcgcca
    14281 gcgccaatct tgacgtggcc gacgacgccg acctggccgc cgagaaccac gaaatcgccg
    14341 agttccgcgc tcccggaaat gccggtttga gccacgatga cgcaatgcct tccaatcacc
    14401 acgttgtgcc cgatttgcac gagattatca attttggtgc cctccccgat cacggtgtcc
    14461 cgcaaggcgc cccggtcgat cgtggtattg gcgccgattt ccaccccgtc ttgaatgatg
    14521 acgcggccga tctgcggcac cttcaggtgg ccattcttgc ccatcgcaaa gccgaaccca
    14581 tcctggccga tggcaacgcc agcgtgaatg gtcacctggt tgccgacgag cgcatgggtg
    14641 atcgaagcat tgggtccgat atagcagtcg cgtccgatat gaacgcgaaa gccgatcaca
    14701 ctgccggcgg caatggtggt cccgcgcccg atgcgtgctt cgggtgcaat cacagcaccc
    14761 ggctcgacga tcgtcccggg ctcgagcgcg gcactcggat gaaccgaagc cgggccatcc
    14821 ccgccatcgg tcagcgcggc cttgggcttg acggcatcgg gatagaacag gaggagcgca
    14881 cgcgcgaaag cgcgataggg ctcaggtgta atcaggcagg cggtgccggc cggtgcctgg
    14941 tcggcgaatt tgggcgcgac caggcaggcg cttgctgcgg tcctggcaaa gagcgacagg
    15001 tatttcggat tgtcgagaaa cgagaggtcg cccggccccg cgttgtccaa gggccggaca
    15061 tccttgatgg cggtgcgaag atcggtgccg ccggccggct tggcgtgcgc ggcctcggcg
    15121 acagtcccca aggcgaaagg gccggcacgt tcgaagaagc ctggatgttc catcaatttc
    15181 tgtggagacg gggcagcgct gcggaatggg ccatagcgcg aagccatagc cgcaaatgac
    15241 ccgttttaga agttcgtcga agcgccaaag cggaagaatt cggtcttatc gaaatcagcc
    15301 ttgagcaatg cctcggcgaa atctgcgcgg agggggccga gcggcgactg ccagatcagg
    15361 ctgaaacccg tggaaaggcg cacctcggtg ctatcgagga tgaagcttcc ctcgtcggcg
    15421 accgcgtcca gcgctagccc gcttggctgc cacagcgatc cggcgtcgac gaaaacggcg
    15481 gcttgcatgc cgaggctttc gggaatgtag gggatcggga aacggacctc cgcggt
//
LOCUS       DASUQP010000264         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1493144, whole genome shotgun sequence.
ACCESSION   DASUQP010000264 DASUQP010000000
VERSION     DASUQP010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493144"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..316
                     /locus_tag="VFY74_11960"
     CDS             5..316
                     /locus_tag="VFY74_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEX5932442.1"
                     /translation="MRRAWPGLPGRPDLVFSGSRKVIFVHGCFWHRHPGCYLTTTPKT
                     RASYWRQKFRQNKRRDYKAVEALEHSGWLVLVVWECETDDIKTLESRLSRFLRDRKSK
                     V"
     gene            447..1538
                     /gene="bioB"
                     /locus_tag="VFY74_11965"
     CDS             447..1538
                     /gene="bioB"
                     /locus_tag="VFY74_11965"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX5932443.1"
                     /translation="MSGAILKQSAARHPVSEISEIANRLSGKAHHAESLPERDRASET
                     RHDWATQDVLDLLCQPLLDLVDEARNVHRRYHADGEVQLASLLSIKTGACPEDCKYCA
                     QSAHYAKTTGLKRETLLDVDEVLEKARRAKDAGATRFCMGAAWREVKDGEAFDSVLAM
                     VRGVRALGMEACVTLGMLTPSQAGRLAEAGLTAYNHNLDTSPEFYGEIITTRTYQDRL
                     ETIERVRKAGIEVCCGGIIGMGETLADRARLLQTLANLKPHPESVPINALVAVPGTPL
                     ADRPQVDPLDLVRMVAAARIMMPLSRVRLSAGRRELSKEAQILCFLAGANSIFYGDKL
                     LTAANNDAADDLALIEEAGLSFKTPGPTG"
     gene            complement(1535..1939)
                     /locus_tag="VFY74_11970"
     CDS             complement(1535..1939)
                     /locus_tag="VFY74_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932444.1"
                     /translation="MTSASLLIVLIVGIAVGALTGLAVGTSLGGLSTAILAGFLGTIA
                     GAIVRNFILSRGAGVGPDDSRTPLLVIVYAAVASLAASAAALELAQRSDLGDSPVWIG
                     TLAGLIAAILMAMLMITYHTRPGEAPTLRSRR"
     gene            2126..>2692
                     /locus_tag="VFY74_11975"
     CDS             2126..>2692
                     /locus_tag="VFY74_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5932445.1"
                     /translation="MPRIVYVNGRYVPEEEAKVSVFDRAFLFGDAVYEVTAVLGGRLV
                     DFEPHLARLDRSLREIALVAPVSHEALLALHLELVRRNEIDEGIVYLEITRGAAERDF
                     AYPDRPEPNVVAFTQNRPLVANPQAETGVKIITIPDFRWKRRDIKSTSMLAQAMGKQE
                     AKLRGAYEGWMVEDGVVTEGTSSSAFILD"
BASE COUNT          502 a          867 c          846 g          477 t
ORIGIN      
        1 cgaaatgcgc cgtgcttggc ccgggcttcc cggccgaccc gatctagtgt tttctgggag
       61 ccgaaaggta attttcgtcc acgggtgctt ttggcatcgc caccccggtt gctacctgac
      121 tacgacaccc aaaacgaggg cttcttactg gcggcaaaag ttccgccaaa acaagcgccg
      181 cgattacaag gcggtcgagg ctctagagca ctcaggctgg ttggtgttgg ttgtttggga
      241 atgcgagacg gacgacatca agacgctcga gagccgcttg tccaggttcc taagggaccg
      301 aaaatccaaa gtatgatcga attgctagtt tctagcttag agactcactt ttcgccgggc
      361 cgctctaggt aatgtcccag gccgaggccc ggcgagggtt cgcctttcgg gcggtccagg
      421 tctttcgttg ccgcccccga tcctgtatga gcggggctat cctcaagcaa tcggcagcgc
      481 gccatcccgt gtcggaaatc tcggaaatcg cgaatcgtct cagcggcaag gcgcatcacg
      541 cggagagtct gcctgagcgt gatcgcgcgt ccgaaacgcg ccacgactgg gccacccagg
      601 acgtgctcga cctcctctgc caacccctgc tcgacctggt cgacgaagcg cgcaacgtgc
      661 accggcgcta tcacgccgat ggcgaggtgc agctcgcgag cctgctttcg atcaagaccg
      721 gcgcttgccc cgaggactgc aaatattgcg cgcagtcggc ccattacgcc aagacgaccg
      781 gcctgaagcg cgaaaccctg ctcgacgtcg acgaggtcct tgagaaggcc cggcgcgcaa
      841 aggacgccgg cgccacgcgc ttctgcatgg gcgcggcttg gcgcgaggtg aaggacggag
      901 aagccttcga cagcgtgctc gccatggtca gaggcgtccg cgcccttggt atggaggctt
      961 gcgtcaccct cggcatgctg acgccgtcgc aagccggccg cctcgccgag gccgggctca
     1021 cggcctacaa ccacaatctc gatacgtcgc ccgagttcta tggcgagatc atcaccacgc
     1081 gaacctacca ggaccgcttg gagacgattg agcgggtgcg caaggccggc atcgaggtgt
     1141 gctgcggcgg catcatcggc atgggcgaga cgcttgccga ccgcgcgcgg ctgctgcaga
     1201 cgctcgccaa cctcaagccg catccggaaa gcgtgccgat caacgccctc gtcgccgtgc
     1261 ccggcacgcc gcttgctgac cggccgcagg tcgatccgct cgacctcgtg cgcatggtgg
     1321 cggcggcacg catcatgatg ccgctgtcgc gggtgaggct ctcggcaggg cggcgcgaac
     1381 tctccaagga ggcgcagatc ctctgcttcc tcgccggcgc caactcgatc ttttacggcg
     1441 acaagctctt gaccgccgcc aacaacgacg ccgcggacga tctcgcgctg atcgaggagg
     1501 ccgggctcag cttcaagacg cccgggccga caggctagcg ccgcgagcgc aaggtcggcg
     1561 cctctccggg cctggtatga taggtgatca tcagcatcgc catcagaatg gcggcgataa
     1621 gaccggccaa ggttccgatc cagaccggcg aatcgccaag atcgctgcgt tgcgcgagct
     1681 cgagcgccgc ggcgcttgcc gcaagcgagg cgacggcggc atagacgatc acaaggagcg
     1741 gcgtgcggga atcgtccggt cccactccgg ctccgcggga gaggataaag ttccggacaa
     1801 tcgcgccggc aatcgtgccg agaaacccgg cgagaatcgc cgtcgataag ccgccgagcg
     1861 aggtgccgac ggcaaggccc gtcagggcgc ccaccgcgat gccgacaata aggacaatca
     1921 acaagctcgc tgaagtcatt tccggttccc cccttatggc tccctgattt cagggccatc
     1981 ctctcgttga aggaatcgat tatgccctaa agcttgggaa tcgtaaaagc aaccgcgggc
     2041 cgctgtttcc cggcttcggt cgcttgcgcc gaaccttggc cggcgctagg caaggtctct
     2101 gcatttgtct caggctttcc cgctcatgcc ccgcattgtc tacgtgaacg gccgttatgt
     2161 gccggaggaa gaggccaagg tatcggtctt cgaccgcgcc tttctattcg gcgatgcggt
     2221 ctacgaggtg acggccgtgc tcggcggacg gctcgtcgat ttcgagcctc accttgcccg
     2281 cctcgaccgc tcgcttcggg agattgccct tgtggcgccg gtaagccatg aggctttgct
     2341 cgcgctccat ctggagctcg tccggcgcaa cgaaatcgac gaaggtatcg tctatctcga
     2401 aattacgcgc ggggcggccg agcgggattt cgcctatccc gataggcccg agcccaatgt
     2461 cgtcgccttc acccagaacc ggccgctcgt tgccaaccct caagccgaga cgggggtcaa
     2521 gatcatcacc atcccggact ttcgctggaa gcggcgcgac atcaagtcga cgtcgatgct
     2581 ggcgcaggcc atgggcaagc aggaagcgaa gcttcgaggc gcctatgaag ggtggatggt
     2641 cgaggacggc gtggtgacag agggaacctc gtccagtgcc ttcattctcg ac
//
LOCUS       DASUQP010000265         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1221539, whole genome shotgun sequence.
ACCESSION   DASUQP010000265 DASUQP010000000
VERSION     DASUQP010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221539"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(479..949)
                     /locus_tag="VFY74_11980"
     CDS             complement(479..949)
                     /locus_tag="VFY74_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5932446.1"
                     /translation="MDKAPEAFRTISEVAEELAVPKHVLRFWEAKFAQLKPMKRGGGR
                     RYYRPEDVALLRGIRFLLYSDGYTIRGVQKILREQGPRFVMDYRRSTENEHRIAPLVV
                     PVEIPDIAAETAQGRPAMRAKSRSGERASVEVETLGALVEEIEACHRILALAKE"
     gene            complement(989..1306)
                     /locus_tag="VFY74_11985"
     CDS             complement(989..1306)
                     /locus_tag="VFY74_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX5932447.1"
                     /translation="MSGKTLTRADLAEAVFQKVGLPRNESAEIVELVLREIIASLERG
                     DTVKLSSFGSFGIRDKEQRIGRNPKTGQEVPITPRRVLVFRASNIMKQRINDALSRRK
                     AAE"
     gene            complement(1443..2339)
                     /locus_tag="VFY74_11990"
     CDS             complement(1443..2339)
                     /locus_tag="VFY74_11990"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX5932448.1"
                     /translation="MVDTSDQWIAERTGIRERRIAAEGELTSSMGAMAARAALDDAGL
                     APEDIDLIILATSTPDQTFPASAVTIQAELGITHGAAFDVQAVCSGFVFALATADSYL
                     KSGQFKRALVIGSEAFSRILDWQDRGTCVLFGDGAGAVVVEAQKLNGALTDRGILAIS
                     LRSDGRYRDKLYVDGGPASTGTVGYVRMDGPEVFRHAVTKISEVIGATLTASGYQASD
                     IDWFVPHQANKRILDGAARKLGLDPARIIITVDKHANTSAASIPLALAQGRADGRIKA
                     GDLVLLEAMGGGFTWGAVLLRW"
BASE COUNT          410 a          747 c          740 g          444 t
ORIGIN      
        1 gagctgcgct acgctccgcc tcgagaatca gatatttaat ctgcacgggt ttggcaacac
       61 agacagcaaa ctgcggccaa tccgactagc cagccgatag gacgccagtc gggccgggct
      121 tatcgtcatg aaccgaggcg aatttctcag cagggtcggc ggctaacgat ctctgcgcta
      181 taaaagaagc ggataaaccc acacagaacg cggggggtgg gttgcagcaa ctcgaacgcc
      241 aagcctccca tccgacggag accgccgagc ggctgtggtt tgcgacaatg cagggcaaga
      301 tgcacgatga cccgattgtg tatgcgacgc gcgattgggc gtcttggctg gtggcggctt
      361 gcgttttggt gtcgttcgtc gctgcttact aggctggcgg tagcttcgct cgcatcggac
      421 taggatgcgc tctgccgtgg ctcggtgacc ttggtcgctg ggcggcgagc ggcgtcttct
      481 actccttggc caaagcaagg atccgatggc aggcctcgat ttcttcgacg agcgctccga
      541 gtgtctcgac ctcgacgctc gcgcgctcgc ccgatcggga tttggcccgc attgcggggc
      601 gtccttgcgc ggtctccgcg gcgatgtcgg gaatctcgac cggaacgacc aacggcgcga
      661 tccggtgctc gttctcggtg ctccgccgat agtccatgac gaagcgcggt ccctgctcgc
      721 ggaggatctt ctggaccccg cggatcgtgt agccatcgct gtaaagcagg aagcggatgc
      781 cgcggaggag cgcgacgtcc tcgggccggt aatagcgccg gccgccaccc cgcttcatgg
      841 gcttgagttg cgcgaacttg gcctcccaga agcgcaacac atgcttcgga accgcaagct
      901 cttcggcaac ctcgctgatt gtgcgaaacg cctcaggcgc cttgtccacc cgtcctcctc
      961 tccggcatca agagccgcgc cggtcagctt attccgccgc ctttctccgc gacagcgcgt
     1021 cgttgatgcg ctgcttcatg atgttgctgg cccggaacac gagcacgcgc cgcggcgtga
     1081 tcgggacttc ctggccggtt ttcggattgc gtccaatgcg ttgctccttg tcgcggatcc
     1141 cgaaggagcc gaacgacgac aactttaccg tgtcgccgcg ctcgaggctg gcaatgatct
     1201 cgcggagcac cagctcaacg atctctgccg attcgttgcg cggcaaacca accttctgaa
     1261 acacagcttc cgccaagtcg gcgcgcgtca gtgtcttccc gctcatttgc gtgcgctccc
     1321 ccagtcgcgg gactattcaa gatgctaatc gagcgagtgc gatgcggtca acctgtcccg
     1381 ctatcaaatt cctgatattg cgacactttc tatcaaggtg tggcattcgc gcggcacccc
     1441 gcctaccagc gcaggaggac cgcgccccac gtaaatccgc cgcccatggc ctcaagcagg
     1501 acaaggtcgc cggctttgat acggccgtcc gcccgccctt gcgccaacgc caaggggatc
     1561 gaagccgccg acgtattggc gtgcttatcg acggtaataa taatgcgcgc agggtcgagc
     1621 ccaagcttcc gcgcggcgcc gtccaagata cgcttgttgg cctgatgcgg tacgaaccag
     1681 tcgatatcgg acgcttgata gccggaggcc gtcaaagtcg ccccgatcac ttcgctgatc
     1741 ttggtcacgg cgtggcgaaa gacctcgggg ccgtccatcc gaacatatcc gaccgtgccg
     1801 gttgaggccg gaccgccatc gacatagagc ttgtcgcgat agcgtccgtc ggagcgcagg
     1861 ctgatggcga ggatgccgcg atcggtgagc gcgccgttca gcttttgggc ctcgaccacc
     1921 acggcgccgg cgccgtcgcc gaacaggacg cacgtcccgc gatcctgcca gtccaaaatg
     1981 cgggagaaag cctccgaccc gatcacgagc gcgcgcttga actggccgct tttcagatag
     2041 ctgtcggcgg tggcgagagc gaagacaaag cccgagcaga cggcctgcac gtcgaatgcg
     2101 gcgccgtgcg tgatgccgag ctcggcttgg atggtgaccg cgctcgcggg gaaggtttgg
     2161 tcgggggtcg atgtggcgag aatgatgagg tcgatgtcct cgggcgcgag ccccgcgtcg
     2221 tcaagggctg cgcgggccgc catcgcaccc atcgatgagg tcagctcgcc ttcggcggcg
     2281 atccggcgct ccctgatgcc ggtccgctcg gcgatccact ggtcggaggt gtcgaccatt
     2341 c
//
LOCUS       DASUQP010000266         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1766062, whole genome shotgun sequence.
ACCESSION   DASUQP010000266 DASUQP010000000
VERSION     DASUQP010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766062"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VFY74_11995"
     CDS             <1..1134
                     /locus_tag="VFY74_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5932449.1"
                     /translation="ASIGGPGSLARPIAVSLGLGLGLLVAVALIHRSLLTEIASSIEV
                     DAPAYYFLDVEEADLPTFYATVKAVEPNAKMADAPMLRGRIVALNGVPADKVKAAPDS
                     RWVLSGDRGLTYTDTVPPASTIEQGEWWPKGYTGPPLVSFDGDLAEGLGLKLGDKITV
                     NILGRNIEATIASLRKIDWESLAINFVMVFSPNTLEGAPHRMLTTLEFPKGTPPEREG
                     KVIQALAEAYPLVTAIKVGDIVDAAKDVLGKVMAAISATAGVTLLIGAAVLAGAVAAG
                     QERRKYLAVIYKTLGATRSRIIKAELLEFGLPGLATALLAVLIATLTAWALCKWAFEV
                     DFLFSPLAAAETVLLALAFVLSIGAVATWLVLSAKAAPYLRAE"
     gene            1255..2010
                     /locus_tag="VFY74_12000"
     CDS             1255..2010
                     /locus_tag="VFY74_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX5932450.1"
                     /translation="MADLDRRHAQGTTVARTEAGVVDQGLRAYMLRVYNYMASGVAIT
                     GVVAYLIYAMAVEQTAAGPQLTAFGSFMFASAFKWVVIFAPLAMVFFISARIGSMSLS
                     AAQIAFWVFAALMGASISSIFLVYAGASIAQTFFITAAAFGALSLWGYTTSRDLSGWG
                     SFLFMGLIGIILASLVNLFIGSTALQFAVSVIGVLVFAGLTAYDTQQIKEMYYVGDDG
                     TVAGRKAVMGALRLYLDFINLFMLLLQLFGDRR"
     gene            complement(2067..>2348)
                     /locus_tag="VFY74_12005"
     CDS             complement(2067..>2348)
                     /locus_tag="VFY74_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX5932451.1"
                     /translation="RRELTQILDIYGRKVASGEWRDYAIDTLKEKAVFSVFRRASETP
                     LYRIEKAPKLARRQGAYSVIAATGLILKRGSDLGRVLRALDRGLRLVEA"
BASE COUNT          372 a          784 c          744 g          448 t
ORIGIN      
        1 gcatcgatcg gcggaccggg ctcgcttgcg cggcccatcg ccgtgtcgct cgggcttggc
       61 ctcggcctgc tcgtcgcggt cgccctgatc caccgctcgc tgctgacgga gatcgcgagc
      121 agtatcgagg tcgacgcgcc cgcttactat ttcctcgacg tcgaggaggc ggacctcccc
      181 accttctacg ccacggtgaa ggccgtcgag cctaacgcca agatggccga tgcgccgatg
      241 ctgcgcggcc gcatcgtggc gctgaacgga gtgccggccg acaaggtgaa ggccgcgccc
      301 gactcgcgct gggtgctctc gggcgaccgc gggctgacct ataccgacac ggtgccgccc
      361 gcctcgacca ttgagcaagg tgaatggtgg ccgaaaggct atacaggccc gccgctcgtc
      421 tccttcgacg gcgacctcgc cgaaggtctc gggctgaagc tcggcgacaa gatcaccgtc
      481 aacattctcg gccgcaacat cgaggcaacg atcgcgagcc tgcgcaagat cgactgggag
      541 tcgcttgcca tcaacttcgt catggtgttc tcgccgaaca cgctcgaagg cgccccccat
      601 cgcatgctga ccacgctcga attcccgaag ggcactcctc ccgagcgcga gggcaaggtg
      661 atccaggctc tggccgaggc ctatcccctc gtcaccgcga tcaaggtcgg cgatatcgtc
      721 gacgcggcca aggacgtgct gggcaaggtc atggccgcga tcagcgccac ggccggcgtc
      781 acgcttctga tcggcgccgc ggtgctcgcc ggcgccgtgg cggcgggtca ggagcggcgc
      841 aaatatctcg cggtcatcta caagaccctc ggagccacgc gatcgcgcat catcaaggcc
      901 gagctcctgg agttcggcct gcccgggctt gccacggcgc tgcttgcggt tctgatcgcc
      961 acgctgaccg cctgggcgct gtgcaaatgg gccttcgagg tggatttcct gttctcgccg
     1021 cttgcggcgg cggagacggt gcttttagcg ctcgccttcg tgctttccat cggggccgtc
     1081 gccacctggc ttgtgctctc ggccaaggct gcgccttatc ttcgcgccga ataaggcggg
     1141 cggacgtccg tcgtcacgcg gccatcttgc gaagccaggc tcactccccc atattcgaag
     1201 ccaaaatgcc taccgagaac cggtcatatc gctaggggaa aggatactgg gacaatggca
     1261 gatctcgaca gacgtcacgc gcaaggcacg acggttgcac gcaccgaggc gggcgtcgtc
     1321 gaccagggcc tcagggccta catgcttcgc gtctacaatt acatggcgag cggcgtggcg
     1381 atcaccggcg tggtcgccta cctcatctac gccatggcgg tggagcagac ggccgcgggc
     1441 ccgcagctca ccgccttcgg ctcgttcatg ttcgcgagcg ccttcaagtg ggtggtgatc
     1501 ttcgcccccc tcgcgatggt gttcttcatt tcggcgcgca tcgggtccat gagcttgagc
     1561 gcggctcaga ttgcgttctg ggtgttcgcg gcgctcatgg gcgcctcgat ttcctcgatc
     1621 ttcctcgtct atgccggcgc gagcatcgcg cagacgttct tcatcaccgc ggcagcgttc
     1681 ggggctctca gcctgtgggg ctataccacg agcagggatc tctcgggctg gggctcgttc
     1741 ctgttcatgg gcctcatcgg catcatcctc gcctcgctgg tgaacctctt cattggctcg
     1801 accgcgctgc aattcgccgt gtcggtgatc ggcgtgctgg tgttcgccgg ccttaccgcc
     1861 tacgacacgc agcagatcaa ggagatgtac tatgtcggcg acgacggaac ggtcgccggc
     1921 cgcaaggccg tgatgggagc gctcaggctc tatctcgact tcatcaacct gttcatgctg
     1981 ctgttgcagc tcttcggcga ccgccgctag acgctgaacc agcaaaaaca tcaaggcccc
     2041 ggtcaaaagc cggggccttt cgtttgtcag gcttcgacga ggcgcaagcc gcgatcgagc
     2101 gcgcgaagca cgcgcccgag atccgagccg cgcttcaaga tcaggcccgt cgccgcgatt
     2161 acgctgtagg cgccctgccg ccgtgcgagc ttcggcgcct tttcgatgcg gtagagcggt
     2221 gtttccgacg cgcgccggaa caccgagaaa acggctttct ccttcagcgt gtcgatggcg
     2281 tagtcgcgcc actcgccgct tgcgaccttg cgcccgtaga tgtcgaggat ttgggtaagc
     2341 tctcggcg
//
LOCUS       DASUQP010000267        21394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1903575, whole genome shotgun sequence.
ACCESSION   DASUQP010000267 DASUQP010000000
VERSION     DASUQP010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 21394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21394
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903575"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VFY74_12010"
     CDS             complement(<1..119)
                     /locus_tag="VFY74_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX5932452.1"
                     /translation="MPPSRNKRSLTIARHRTSVTVEEPFWDALGEIARAEGKSV"
     gene            complement(137..1564)
                     /gene="fumC"
                     /locus_tag="VFY74_12015"
     CDS             complement(137..1564)
                     /gene="fumC"
                     /locus_tag="VFY74_12015"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5932453.1"
                     /translation="MPQDRGARRGSPTSARIETDSLGPIEVAHDTYWGAQTERSRRNF
                     RIGEDIMPKSLIRAIAMVKKAAAQTNREIGLLEQRLAHAIVAAADEVIEGKLDSHFPL
                     VVWQTGSGTQTNMNVNEVIANRASELLGETLGSTRPVHPNDHVNLGQSSNDVFPTAMH
                     IAAAEEIAYRLNPALSRLYQALKDKAEAFQDILKIGRTHLQDATPLSLGQEFGGYAAQ
                     IDHGIERIKVTLPGLYALAQGGTAVGTGLNTSPLFAESFAAKIAAMTKLPFVSAENKF
                     EALATHDAFVFAHGALNTVAVSLFKIASDIRLLGSGPRSGFGELSLPENEPGSSIMPG
                     KVNPTQCEAVTMVAARVFGNNTTITFAGSQGHFELNVFKPVIAHAMLQSIRLLTDVAD
                     SFREKCIVGIEPNRSVIKAHLERSLMLVTALAPHIGYDKASEIAKSAHKRGTTLREEA
                     IRLGHVNAEDFDRLVRPEDMTGPKR"
     gene            1738..3663
                     /locus_tag="VFY74_12020"
     CDS             1738..3663
                     /locus_tag="VFY74_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX5932454.1"
                     /translation="MAEVPVRMALPAPRAELMSLLLARLAALIVALIGGLILLAWQLP
                     IPRLTEAFPGLASMTPAVAGGFVAAAIGLFCLTFRPIRLISRVIGVALVVLGLAIFSQ
                     DLFGFQLGLDQALIRADSAGTAQTEDLRLAPGVTGLLIIFGLALLYAKQGGITSAFSQ
                     VLALIIIGQILIVLASYAYGVADDYYAFPFTHMSMYGAVCGLLLGLGLLAASPEYGIG
                     AAIVEQSPAGEMLRRLLPGIIVLPLVIGWFAHMAETRALYDSAATLAIFAVASIFVLA
                     LFAWTTIGAVRRADRDRNAALMELRGQREWLSTTLASIGDGVIATDPNGSVLLLNKIA
                     EQFTGWSGASARGRPIWEVFRVINENTRKPADDPALKALRERTVTRFESGALLVTRDK
                     QELPVEHCGAPIIGFDGSLAGAVLIFRDVTERRRTAQRQSMLVGELNHRVKNALAIVQ
                     SLVQASLRQATSQQAKEMAQTLAERLQALHRAHDLLLESQWSGASLKAMVERELEPYR
                     REGGPKITVKGSDVLLPPQCTSILAMTLHELATNAVKYGALSSNSGQLAISWKTARGG
                     RLNLTWEERATLAQTPARRNSGFGMQLIDKGVRHNLGGDTKVEFRPHGLYVELHVPLE
                     PSKEPRAAPQKAAALSA"
     gene            complement(3732..4274)
                     /locus_tag="VFY74_12025"
     CDS             complement(3732..4274)
                     /locus_tag="VFY74_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX5932455.1"
                     /translation="MADDEIRYDLLTQDALRGVVRQVLARVQREGLPGEHHLYIAFDT
                     RADGVSVSKRLKEQYPEEMTIVLQYQFWDLQVTNERFEVKVSFSNVPERLVVPFSAVK
                     AFYDPSAEFGLQFGKPGAANDSARQHMAAALPDLVGEPEEPRGALEPPHDEGGETHSA
                     EVVLSRPPAEVVQLDRFRKK"
     gene            complement(4329..4741)
                     /gene="ssrA"
                     /locus_tag="VFY74_12030"
     tmRNA           complement(4329..4741)
                     /gene="ssrA"
                     /locus_tag="VFY74_12030"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            complement(4803..5042)
                     /locus_tag="VFY74_12035"
     CDS             complement(4803..5042)
                     /locus_tag="VFY74_12035"
                     /inference="COORDINATES: protein motif:HMM:NF025051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX5932456.1"
                     /translation="MRTIPALFALSFALAAPAAAQPVTIEDVRGMAFDKGIVKIEKVE
                     LDDGIWEVEGDDASGNEIEMKVEAGSGQIIKLERD"
     gene            5162..5956
                     /locus_tag="VFY74_12040"
     CDS             5162..5956
                     /locus_tag="VFY74_12040"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5932457.1"
                     /translation="MRQYLDLLTRVRKSGTRKQDRTGTGTLAVFGHQMRFDLEAGFPL
                     VTTKKLHVKSIVHELLWFLQGSTNVRYLNEHGVTIWDEWADENGELGPVYGRQWRSWP
                     APDGRQIDQIAAVVEGLKRDPDSRRHIVSAWNVGELDKMALFPCHCLFQFYVADRRLS
                     CQLYQRSADVFLGVPFNIASYALLTLMMAHVTGLAPGEFVHTFGDVHLYLNHLDQADE
                     QLKRTPRALPRMEINPEVRSLFDFAYEDFKLVGYDPHPAIGAKVAV"
     gene            6039..6560
                     /locus_tag="VFY74_12045"
     CDS             6039..6560
                     /locus_tag="VFY74_12045"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX5932458.1"
                     /translation="MTHKQPIIALVVAMGRNRAIGKGGGLPWRLSSDMRYFRKVTMGK
                     PIVMGRRTFSSLPRVLDGRLNIVLTRNPSFEAPGVVVADNLEQALDVARREARRTGVD
                     EIMVIGGEEIFREVLPQAGRIYLTEVQAAPDADTWFPEHDASEWREVSREDHAPGPKD
                     DHAFSFVVLERKV"
     gene            6736..7851
                     /gene="hflK"
                     /locus_tag="VFY74_12050"
     CDS             6736..7851
                     /gene="hflK"
                     /locus_tag="VFY74_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX5932459.1"
                     /translation="MPWSDQTGGGWKGGGGGPWGPRQSPGQQPDLEEILRRSQDRLRQ
                     AIPGGRMGGPLAILLTVVVAALVGFFGFTVRVNPDELGIVLRFGEYVRQLPPGLNFRW
                     PYPIEQVELPKVTRINRIEIGMRGSSDVRDASSTIRDVPEESLMLTGDENIVDVDFVV
                     FWRIADAPDFLFNIQNPEGTVKAVAESAMREIVGQNDIEPILTEARAKTEFDVQQLMQ
                     KVLDSYGAGVQVTQVQLQKVDPPAQVIDAFRDVQAARADQERLQNEAQAYANRIIPEA
                     RGEAERILQAAQGYRDQVIAEAKGQGDRFTKVYEQYKKAPDVTRQRIFIETMEKVLGN
                     ADKVIVDDKAGPGVVPFLPLSEFTTRPAPAQPQEGQP"
     gene            7848..8882
                     /locus_tag="VFY74_12055"
     CDS             7848..8882
                     /locus_tag="VFY74_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX5932460.1"
                     /translation="MNRAVLTVFLVILGTVAVIAYLTLFTVTQTQQALVLEFGKPKRV
                     ITTPGLQYKIPFIQNVEFFDKRILDIDTASQEVIASDKKRLVVDAFARYRITDALLFF
                     QTLRDERIASARIGAVLEASLRRVLGGSSFTAVVRDKRESLMKTILGQVNQEATPFGV
                     KIVDVRIKRVDLPEANMQAIYRRMQTERQREAAEFRAEGEGAARRIRATADREVTVIK
                     AEATGESERIRGAGDAERNRIFAEAFGPDPDFFAFYRSMQAYEGALRPGDTRLLLSPN
                     SQFFQYFNSSMGKAAQDAIPGPASPPQTPESPSGNAGVPEQGGADASVAFPEPEAPSA
                     ENLPAAPQTP"
     gene            8923..9108
                     /locus_tag="VFY74_12060"
     CDS             8923..9108
                     /locus_tag="VFY74_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX5932461.1"
                     /translation="MSNIVVALGLVLVIEGTLWALAPGLGRRLLQTAAETPESNLRLI
                     GAAAVTIGVLVIWLVRG"
     gene            9198..10682
                     /locus_tag="VFY74_12065"
     CDS             9198..10682
                     /locus_tag="VFY74_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX5932462.1"
                     /translation="MPQDGTPGEPGFRRSRLLAAALALILFAAAVLATPGQARGPDSV
                     AEVAEGLQEAVVNISTTQTLKGSPDSAPTGPGPKGSPFEEFFDDFFDDQGGDGLSRKV
                     SSLGSGFVIDPAGLVVTNNHVIEGADEILVNFTDGTSLKVTKILGHDPKTDLALLQVE
                     PKKPLTAVTFGDSAKMRVGDWVMAIGNPFGLGGSVTVGIISATRRDINAGPYDDFFQT
                     DAAINRGNSGGPLFNMEGEVIGVNTAIISPSGGSIGIGFAVPSNSAVQVLDQLKQFGE
                     TRRGWLGVHVQNVTDEIAASLGLEQAKGALVAKVSPGSPAATAGIQPSDVIVKFDGQP
                     IDNMRSLPRAVAATAVGKSVAVELLRKGETKSFSVTVGQLPEDEEGEEVLQSNAKPAP
                     EPEPDSLLGLTLAPLTDEVRQRYGIGKSIEGVVVVEVKPNSPAAQKDVKPGDVIVEVT
                     QEKVKQPQDVKMRLLAVKKSGRRSVLLLLSDAKGELRFVAVPTG"
     gene            10729..11652
                     /gene="miaA"
                     /locus_tag="VFY74_12070"
     CDS             10729..11652
                     /gene="miaA"
                     /locus_tag="VFY74_12070"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX5932463.1"
                     /translation="MSRPKAILIAGPTASGKSAAALRLAERLGGTVINADSMQVYREL
                     CILTARPVAAELAHVPHLLYGTVPAAETYSVGHWLAAAADAITEAESAGRVPILTGGT
                     GLYFKALVEGLAPVPDVPPEIRAYWRERAAAIGPGQLHRELGSRDPAMAARLTATDPQ
                     RLVRALEVIDATSISLAEWQGASAAPVLGPEGVVKLVIAPEREALYAAIDARFDAMVE
                     AGAIGEVRSLLALDLDPGLPAMRAHGVRELALYLAGRSSRADAIAKAKTETRRYAKRQ
                     MTWLKRFMKDWDWVPDAGSAVDAARAQIATR"
     gene            complement(11642..13561)
                     /locus_tag="VFY74_12075"
     CDS             complement(11642..13561)
                     /locus_tag="VFY74_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX5932464.1"
                     /translation="MARLNSETQALAGANGDLADVRKQFWALTFGSIGVVYGDIGTSP
                     LYALREAVLAASSDGGTVTPAAIYGVLSLIFWSLIVVVTIKYVLILLRADNNGEGGTL
                     ALTALAFRALGRRTPLVIALGTVGAAMFYGSTLITPALSVLSAVEGLQVATAAFGPYV
                     LPITVAILVALFSVQSHGTARVSTLFAPITTVWFLVIAVAGAVEIAANPAVLSALNPI
                     YGLNFLFTHGIIGLVTLGAVFLVATGSEALYNDLGHFGRKPIQTAWLYFVLPALLFNY
                     FGQGALLLERPETIENPFYLLYPDWALYPMVALATLATAIASQAVISGAYSITRQAMQ
                     LGFLPRMEVRHTSEALAGQIYMPRVNYLLLTGVLLLVFMFRSSSELAAAYVLAVATTA
                     LAASLLGFIVIWKEWKWRLWTTAAVMVPLIFADSSFVIASLLKLIEGAWLPVLVGAIL
                     ILIMTTWRRGTRLLALKTRRVEVPFKPLVKSLEERPPNIAPGTAVFLTSDPEFAPTAL
                     LHNLKHNKVLHEHNVILTIVNEDIPRVNPQVRVRLEPISDRFMRLIMHFGFMERPNVP
                     RALALARKQGWHFDIMSTSFFLSRRSVRPDPRSGMPAWQDRVYIFLAHNADDASSYFQ
                     LPTDRVVEIGTQVTV"
     gene            complement(13568..15488)
                     /locus_tag="VFY74_12080"
                     /pseudo
     CDS             complement(13568..15488)
                     /locus_tag="VFY74_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331450.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
     gene            15860..17626
                     /locus_tag="VFY74_12085"
     CDS             15860..17626
                     /locus_tag="VFY74_12085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HEX5932465.1"
                     /translation="MSRAMTGAEMVLQALADQGVEHIFGYPGGAVLPIYDHLFQQDRL
                     KHILVRHEQGAVHAAEGYARSTGKVGVVLVTSGPGATNAVTGLTDALMDSIPIVCITG
                     QVPTHLIGNDAFQECDTVGITRPCTKHNYLVKDVNALSRVLHEAFYVARTGRPGPVVV
                     DIPKDIQFARGEYTGLTNSQHKSYNPRVKGDERAVAQAIELMARAKRPLFYTGGGIIN
                     SGPLASARLRELVRMTGFPITSTLMGLGAFPASDPRWLGMLGMHGTYEANNAMHDCDL
                     MVCIGARFDDRITGRIDAFSPGSTKIHVDIDRSSINKNVKVDLPIVGDCAHVLEQMID
                     AWKAKKPKLDKAALKVWWAEIDRWRARNCLKYRKRDDVIMPQYAIERLYALTRDQDVY
                     ITTEVGQHQMWAAQFFKFEEPNRWMTSGGLGTMGYGLPSAVGVQVAHPGSLVIDIAGE
                     ASVLMTMQEMSTAAQYDLPIKIFILNNEYMGMVRQWQELLHGGRYSHSYTAALPDFVK
                     LAEAYQGVGIRAEKPSELDDAIREMIRVKKPVIFDCRVDKHANCFPMIPSGEAHNNML
                     MGDDVTEEDIEKAITEEGKVMV"
     gene            17644..18147
                     /locus_tag="VFY74_12090"
     CDS             17644..18147
                     /locus_tag="VFY74_12090"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX5932466.1"
                     /translation="MIRFLLSLRAIMLIGSAGAMIGALLMFFQGGFYLYEAWHTLMTP
                     GHAAERQVTVPVLEAVDAFLFGIVLVIFAYGIAIGFVFTLPEGYGARLPEWMKVGGVG
                     QLKATLAEVVIVVLIVIFARVVVEANGHFQWSILVLPVSIFFIALALRMIELGGGKGG
                     GEGKAAH"
     gene            18160..18717
                     /gene="ilvN"
                     /locus_tag="VFY74_12095"
     CDS             18160..18717
                     /gene="ilvN"
                     /locus_tag="VFY74_12095"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5932467.1"
                     /translation="MTGNPTKQPGSAYPLEKKEERLETHTMTVLVDNEPGVLARVIGL
                     FAGRGYNIESLTVSETEHEAHLSRITIITTGTPMVIEQIQHQLERLVPVHSVTDLTVE
                     GNPLERELAMVKVAGKGEKRVEALRLAEAFRADVIDTSGEHFVFQVTGEQSKIEEFIT
                     LMQPLGLVEVARTGLAAISRGPERM"
     gene            18843..19862
                     /gene="ilvC"
                     /locus_tag="VFY74_12100"
     CDS             18843..19862
                     /gene="ilvC"
                     /locus_tag="VFY74_12100"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5932468.1"
                     /translation="MRVYYDRDADLNLVKGKKVAIIGYGSQGHAHALNLKDSGVKDVA
                     VGLRPGSQSVAKAEKAGLTVMDVPDAGKWAEVVMMLTPDELQAGIWERELKPNMQQGA
                     ALFFAHGLNIHFRLIEPRADMDVCMVAPKGPGHTVRAEYLRGAGVPCLMAIAQDATGN
                     AHDLTLSYAAAIGGGRAGIIETTFREECETDLFGEQSVLCGGLVELMRNGYETLVEAG
                     YSPEMAYFECVHEVKLIVDLIYEGGIANMNYSVSNTAEYGEYVSGPRVVGPASREAMK
                     EILADIQSGKFVKDWMLENRVSQTSFKAMRAKCDNHQMEEVGKRLRAMMPWIAERKMV
                     DKSKN"
     gene            20639..>21394
                     /locus_tag="VFY74_12105"
     CDS             20639..>21394
                     /locus_tag="VFY74_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX5932469.1"
                     /translation="MQKHGGNGLWEPHFLSWHRVFLFFFEAVLKQAAVESRETTAFDL
                     PYWNWYEDPLPQIFKEGDEKTNPLYVRGSLAGGSVDLDKLIPGLSTAPFLSDDFLIAP
                     GVPPENTFLYQLDENPHGTVHDLVAGDMGRVPTAARDPIFWFHHVNLDRLWTAWMKSG
                     SRRLPARDSAWGRTSWFFDPSGNWKATAGDMLNSETSFGYRYDDETLPSVSVAVASAL
                     PSRTLEALPLSAAMAPPMDEAAPSSTATTLSQTA"
BASE COUNT         4040 a         6794 c         6748 g         3812 t
ORIGIN      
        1 accgacttgc cttcggcgcg cgcaatttcg ccaagcgcat cccagaaggg ctcctccacc
       61 gtcacgctgg tgcggtgccg cgcgatggta agcgagcgct tgttgcgaga aggtggcatg
      121 cgccaagcgg gcggcctcaa cgcttcgggc cggtcatgtc ttctgggcgc acgagcctat
      181 cgaagtcctc ggcgttgacg tggccgagcc ggatcgcctc ttcgcgcaag gtggtgccgc
      241 gcttatgcgc ggacttggcg atctcgctcg ccttatcgta gccgatgtga ggcgcaagcg
      301 ccgtcaccag catgagcgag cgctccaaat gcgccttgat gacgctccgg ttgggctcga
      361 tgccgacgat gcatttctcg cggaaactgt cggcgacatc ggtgagaagc ctgatcgact
      421 gcagcatggc gtgcgcgatc accggcttga acacgttgag ctcgaaatgt ccctggcttc
      481 cggcgaaggt gatcgtcgtg ttgttgccga acactcgcgc ggccaccatg gtcacggcct
      541 cgcattgcgt cgggttcacc ttgccgggca tgatcgagga tcccggctcg ttctccggca
      601 ggctcaactc cccgaagccc gagcgggggc cggagccgag cagcctgatg tcgcttgcga
      661 tcttgaacag ggacacggcg acggtattga gcgcgccatg ggcgaacacg aacgcgtcat
      721 gcgtggccag cgcctcgaac ttgttctcgg cactgacgaa gggaagcttg gtcatcgccg
      781 cgatcttcgc ggcgaagctt tcggcgaaga gtgggctggt attaaggccg gttccgaccg
      841 ctgtgccgcc ctgggcgagg gcataaaggc cgggaagcgt gacttttatc cgctctatgc
      901 cgtgatcgat ctgcgccgca tagccgccga attcttgccc taaagagaga ggggttgcgt
      961 cctggagatg cgtgcggccg atcttgagaa tgtcctggaa tgcctcggct ttatctttca
     1021 gtgcctggta aagccgggac agtgccgggt tgagccggta ggcgatttct tcggccgcgg
     1081 cgatatgcat ggctgtggga aagacatcgt tggaggattg accgagattg acgtgatcat
     1141 tggggtggac cggccttgtt gaaccaagag tctcgcccag caattcgctc gcgcggttgg
     1201 cgatgacttc gttcacattc atgtttgtct gggttcccga gccggtttgc cagacgacca
     1261 gggggaaatg ggaatcgagc ttgccttcaa taacctcgtc ggcagcggcg acgatggcgt
     1321 gagccaggcg ctgttcgagg aggccgattt cgcggttggt ttgggctgcc gcctttttga
     1381 ccatggcgat cgcccggatc agggatttcg gcatgatgtc ctcgccgatc ctgaaattcc
     1441 ggcgcgagcg ctcggtctgg gcgccccaat aggtgtcgtg cgcgacctcg atcggtccga
     1501 gagaatcggt ttcgatgcgc gcggaggttg gggacccgcg tctggcacct ctgtcctgag
     1561 gcatgctcaa atctcctgtc gacgccctgc ggcgaaggct ctaccggggc taggggggct
     1621 aggctataac acgctccagg gtgggagaac gcgctcgttc gctgcgttgc ggccttctgc
     1681 cgaggagcgc ccaaagcttc cggctccatt ttccgtgaac ttgggtgagg aaggcgagtg
     1741 gctgaagttc cggtgaggat ggcgctgccc gcgccgcgcg cagagctcat gtcgctgctg
     1801 ctggcgcgcc tggccgcctt gattgtcgcc ctgatcggcg gcctcatcct ccttgcgtgg
     1861 caactgccca ttccgcggct gaccgaagcc tttccggggc tcgccagcat gaccccggca
     1921 gtggccggcg gcttcgtggc agcggcgatc gggctctttt gtctcacctt ccgcccgata
     1981 cggctcatct cccgagtgat cggtgttgcc ctcgtcgtgc ttggtctcgc cattttcagc
     2041 caagacctgt tcggcttcca actcgggctc gatcaggcgc tgatccgggc cgacagcgcc
     2101 ggaaccgcgc agaccgaaga tctgcgcctg gcgccagggg tcacgggtct cctgatcatt
     2161 ttcggcttgg cgctgctcta cgccaagcag ggcggcatca cctcggcctt cagccaggtg
     2221 ctcgcgctca tcatcatcgg ccagatcctg atcgtgctgg caagctacgc ctatggcgtc
     2281 gccgacgatt attacgcctt ccccttcacg cacatgtcga tgtacggcgc ggtctgcggg
     2341 cttcttctcg ggctcggctt gcttgccgcg tcccccgaat atggcatcgg cgcggccatc
     2401 gtcgagcagt cgcccgccgg cgagatgcta cgtcggcttc tgcccggaat tatcgtgctg
     2461 ccgctggtga tcggctggtt cgcccatatg gccgagacac gcgccctcta cgacagcgcc
     2521 gcgacgcttg ccatcttcgc cgtcgccagc atcttcgtgc tggccctgtt cgcctggacc
     2581 accatcgggg ccgtgcgccg cgccgatcgc gaccgcaacg cggcgctgat ggagctgcgc
     2641 ggtcagcgcg aatggctgag cacgacgcta gccagcattg gcgacggggt gatcgccacc
     2701 gatcccaatg gcagcgtgct gctgctcaac aagatcgccg agcagtttac cggatggtcg
     2761 ggagcgagcg cgcgaggccg gccgatctgg gaagtgtttc gcgtgatcaa cgagaatacg
     2821 cgaaagcccg ccgacgaccc ggcgctcaag gcgctgcgcg agcgtaccgt gacgcggttc
     2881 gaatcgggcg cgcttctcgt cacccgcgac aagcaggaat tgccggtcga gcattgcggt
     2941 gcgccgatca tcggcttcga tggctcgctc gccggcgccg tgctcatttt ccgcgacgtc
     3001 accgaacggc gacgcaccgc gcagcgccag tcgatgctgg tgggcgagct caatcatcgc
     3061 gtcaagaatg cgctcgccat cgttcagtcg ctggtgcagg cgagcctgcg ccaggcgacc
     3121 tcgcagcagg ccaaggagat ggcgcagacg cttgccgagc ggctccaggc gctccatcgc
     3181 gctcacgatc ttctgctcga atcgcaatgg tcgggcgcaa gcctcaaggc catggtcgaa
     3241 cgcgaactcg agccctaccg gcgcgagggc ggcccgaaga tcaccgtcaa gggaagcgac
     3301 gtgctgctgc cgccgcaatg cacctcgatc ctcgccatga ccctgcacga actcgccacc
     3361 aacgcggtca aatacggcgc tctctcctcc aattcgggtc agctcgccat tagctggaaa
     3421 accgcgcgcg gcggccggct caatttgacc tgggaggagc gggcgacgct cgctcagacc
     3481 ccggcccggc gcaacagcgg cttcggcatg cagctcatcg acaagggcgt tcgccacaat
     3541 ctcggcggcg ataccaaggt cgagttcagg cctcacggcc tctatgtcga gctgcacgtg
     3601 ccgctcgagc cgtcgaaaga gccgcgcgcg gcgccgcaaa aagcggcggc gctttcggcc
     3661 tgacgctatc ggcttccagc ccacgcgaat ccgagcgggc cgctcagaca gcgccggaat
     3721 tatagctgtg gctatttctt gcggaaccga tcgagttgga cgacttccgc cggagggcgt
     3781 gacagcacga cttcggcgct gtgcgtttcg ccgccctcat cgtgcggggg ctcgagcgcc
     3841 ccgcgcggct cctccggttc gccgacgaga tccggaagcg cggccgccat gtgctggcgg
     3901 gccgaatcgt tggccgcgcc cggcttgccg aactggaggc cgaactccgc cgacgggtcg
     3961 taaaaggcct tgaccgcgct gaagggcacc accagccgtt ccggcacgtt ggagaaggag
     4021 accttcacct cgaagcgttc gttcgtcact tgcaagtccc agaactggta ctgcaacacg
     4081 atcgtcatct cttcggggta ttgctccttg agacgcttgg acaccgacac gccgtccgcc
     4141 cgcgtgtcga aggcgatgta gaggtgatgc tcgcccggaa gtccctcacg ctggacgcgc
     4201 gccaagactt ggcgcaccac gccgcgcaga gcatcctgcg tgagaagatc gtatctgatc
     4261 tcgtcgtctg ccatggaacc cgccgcctgc cctcgcgcga cgtaaggcag gcaatcaccc
     4321 cgtcataaag tggagggctt ctgttgcccg gtgccctccg aaaccgcgcc tagcccctgc
     4381 gaagaggctg ggacttgatg tttaggctat cgcgccgctt acgcagcgag agcagcctcc
     4441 gcatagttgt cattcgcaac tatttggttg gcccgataac ggcggaacca tgccgggcga
     4501 aagcacgacc tttacaccct cgtcgatcct atttcgcccc ccatgtcagg tttcagaaat
     4561 cagttgtcag aaatcagaaa agccccgctg atttttgacc actgatttct gcaccctgat
     4621 ggtggaggcg ccgggtaccg cccccgggtc cgaatggctt attacgaagg ctgtttatcg
     4681 ccatagccag cttgcgccgg caacaatcta tataagcctc tatggcggca aaaaaaagga
     4741 tgttgctgcg acaaccccca gccgcgaatc gaaaccgtgc tatgctggag ggcgccggtt
     4801 cgctagtcgc gctcgagctt gatgatctgg ccgcttccgg cctcgacctt catctcgatc
     4861 tcgttcccgc ttgcgtcgtc gccttcgacc tcccagatgc cgtcgtcgag ctcgaccttc
     4921 tcgatcttca cgatgccctt gtcgaaggcc atgccgcgga catcctcgat cgtcacgggt
     4981 tgggcggcgg cgggagcggc aagggcgaag ctgagagcga aaagagcggg gatggtacgc
     5041 attgaaggca ccttttcgtt ggacgatcgg cgaagctctg tgatcgacag ggggaacgcc
     5101 ctctaggcgg ccccgttccg cgccatcata ggaaaaatcg ttataggaga aatcgttccc
     5161 gttgcgccaa tatctcgatc tcctcacccg cgtgcgcaag agcgggaccc ggaagcaaga
     5221 ccgcacgggg accggcacgc tcgccgtttt cggccatcag atgcgcttcg acctcgaggc
     5281 gggctttccg ctcgtcacca ccaagaagct gcatgtgaag tcgatcgtcc acgagctgct
     5341 ctggttcctg caaggctcga ccaatgtgcg ctatctgaac gagcacggcg tgacgatctg
     5401 ggacgagtgg gccgatgaaa atggcgaact cggcccggtc tacggaaggc agtggcgctc
     5461 atggcctgcg cccgacggac gtcagatcga ccagatcgcc gccgtcgtcg aagggctgaa
     5521 gcgcgacccc gattcgcgcc gccatatcgt cagcgcctgg aacgtgggcg agctcgacaa
     5581 gatggcgctt ttcccctgcc actgcctgtt tcagttctat gttgccgaca ggcgcctctc
     5641 atgccagctc tatcagcgct cggccgatgt ctttctcggc gtgccgttca acatcgcctc
     5701 ctatgccttg ctcacgctga tgatggcgca cgtcaccggc ctcgcgcccg gggagttcgt
     5761 ccatacgttc ggcgacgtcc atctctatct caatcatctc gaccaggccg acgagcaatt
     5821 gaagcggacc ccgcgtgcgc ttccccgcat ggagatcaat ccggaggtgc gctcgctgtt
     5881 cgacttcgcc tatgaagatt tcaagcttgt cggctacgat ccgcatcccg ccatcggcgc
     5941 gaaggtggcg gtgtgactcg tcatggtgag gtgcccggcc gtccttcgag gctcgcgctt
     6001 cgcgctcgca cctcaggatg acggccgggc ctcgaaccat gacgcataag cagcccatca
     6061 tcgcgcttgt cgttgccatg ggccggaacc gcgccatcgg caaaggcggg ggtcttccct
     6121 ggcgcctcag cagcgacatg cgctacttcc gcaaagtcac catgggaaag ccgatcgtca
     6181 tgggccgccg aacgttcagc tcgctcccgc gcgtgctgga cggccgcctc aacatcgtgc
     6241 tcacgcgcaa cccctccttc gaggcgcccg gcgtcgtcgt ggcggacaat ctcgaacagg
     6301 cgctcgatgt cgctcggcgt gaggccagac ggacgggcgt ggacgagatc atggtcatcg
     6361 ggggcgagga gattttccgc gaagtccttc cacaggcggg tcgcatctac ctgacagagg
     6421 tccaagcggc gcccgatgcc gacacctggt tcccggaaca cgacgcgagc gaatggcgcg
     6481 aggtgtcgcg cgaggaccac gctccgggcc ccaaggacga ccacgccttc agcttcgtcg
     6541 tgcttgagcg caaagtctga ggcctgccgg agcaggctcg ccccgcgtca ttgcaccccc
     6601 cacatgagtg gcctataact cttccgaccc tgtcgcagga tcgccatgtt gatccggcta
     6661 gtcttgccgg ctagcccgtt acccacctag gcgtctgcgc cacccccaat cacccgaatt
     6721 caggaccgat tcatcatgcc ttggagcgat caaaccggcg gcggctggaa gggcggaggc
     6781 ggagggccct ggggtccgcg ccaaagcccc ggccagcaac ccgacctcga agagattctc
     6841 cgccggagcc aggaccggct ccgccaggcc ataccgggcg gacgcatggg cgggccgctc
     6901 gccattctgc tgacggtcgt ggtcgcggcc ttggtgggct tcttcggctt caccgttcgc
     6961 gtcaatcccg acgagctcgg catcgtgctc agattcggcg aatatgtgcg tcagctccct
     7021 cccggcctca atttccgctg gccctatccg atcgagcaag tcgagctgcc gaaagtcacg
     7081 cgcatcaacc gcatcgagat cggaatgcgc ggctcttctg acgtccgcga cgcctccagc
     7141 acgatccgcg acgtgcccga ggagagcctg atgctgaccg gcgacgagaa tatcgtcgat
     7201 gtcgacttcg tcgtgttctg gcgcatcgcc gacgcccctg actttctgtt caacatccag
     7261 aatcccgaag gcacggtaaa ggcggtggcc gagagcgcga tgcgcgagat cgtcggccag
     7321 aacgatatcg agccgatcct gaccgaagcc cgtgcgaaaa ccgaattcga cgttcagcag
     7381 ctgatgcaga aggtcctcga cagctacggc gcgggcgtcc aggtgacgca ggtgcagctg
     7441 caaaaggtcg atccgccggc gcaagtgatc gacgccttcc gcgacgttca ggccgcgcgc
     7501 gccgatcagg agcgcttgca gaacgaggcc caggcctatg ccaatcgcat cattcccgag
     7561 gcacgcggtg aggccgagcg tatcctccag gcggcccaag gctatcgcga ccaggtgatc
     7621 gcggaggcca agggtcaggg cgaccgtttc accaaggtct atgagcagta taagaaggcg
     7681 cccgacgtca cccgccagcg catcttcatc gagaccatgg agaaggtgct cggcaacgcc
     7741 gacaaggtga tcgtcgacga caaggcaggt cccggcgtcg tgccgttcct gccgctttcg
     7801 gagttcacga ccaggcccgc tccggcacag ccgcaggagg ggcagccatg aaccgcgccg
     7861 tcctcaccgt cttcctagtc atcctcggca ctgtcgccgt gattgcctat ctcacgctgt
     7921 tcaccgtgac ccagacccag caggctctgg ttctcgaatt cggcaagccg aagcgggtca
     7981 tcaccacgcc cggccttcaa tacaagatcc cgttcatcca gaacgtcgaa tttttcgaca
     8041 agcgcattct cgacatcgac accgcgagcc aggaagtcat cgcgtccgac aagaagcggc
     8101 tcgtggtcga cgcgtttgcc cgttaccgga tcaccgatgc cctgctgttc ttccagacct
     8161 tgcgcgacga gcgtatcgcc agcgcccgta tcggcgccgt tctcgaagcc tcgctgcggc
     8221 gcgtgctcgg cggttcgagt ttcactgcgg tcgtgcgcga caagcgcgag tcgctcatga
     8281 agacgatcct tggccaggtc aaccaggagg cgacaccctt tggcgtgaag atcgtcgacg
     8341 tgcgcatcaa gcgcgtcgat ctgcccgagg ccaatatgca ggcgatctac cgtcggatgc
     8401 agaccgagcg gcagcgggag gcggcggagt tccgcgccga aggcgagggc gccgcgcgac
     8461 gcatacgcgc caccgccgat cgcgaagtga ccgtcatcaa ggccgaggcg accggggaat
     8521 cggaacgcat ccgcggcgcc ggcgacgccg agcggaaccg catcttcgcc gaggccttcg
     8581 gcccggatcc cgacttcttc gcgttttacc ggtcgatgca ggcctacgag ggggcgctga
     8641 ggccgggcga taccaggctt ctgctcagcc ccaactcgca attcttccaa tacttcaaca
     8701 gctctatggg caaggctgct caggacgcga tacccgggcc ggccagtccg ccccaaacgc
     8761 ccgaatcgcc gtcgggcaat gccggggtgc cggagcaggg gggcgcagac gcgagcgtcg
     8821 catttcccga gcctgaggcc ccgagcgccg agaaccttcc cgcggcgcct cagaccccgt
     8881 gaggtcggaa ggcgggcgca agcatggcgg gaagccggcg caatgagcaa catcgtcgtg
     8941 gcgctcgggc tcgtcctcgt catcgaaggg acgttgtggg cgcttgcgcc cgggctaggc
     9001 cggaggttgt tgcagacggc ggcggaaacg cccgaatcca atctgcggct gatcggcgcc
     9061 gcggctgtaa cgataggcgt gctcgtgatt tggctggtgc gcggctgacg ccatttccgc
     9121 tccgcaccga ttctgcttta tcttgtgaag tcggctaaga atcgccgaac atcactgcga
     9181 ggcgggaaag gccttcgatg ccacaggacg gcactccggg tgagccaggt tttcggcgtt
     9241 cccgtttgct ggcggcggcg cttgcgctga tcctctttgc cgcggccgtc ctggccacgc
     9301 cgggccaagc ccgcgggccc gactccgttg ccgaggtcgc cgaggggctg caggaggcgg
     9361 tcgtcaacat ttccacgacc cagacgctga aaggatcgcc cgacagcgct cccaccgggc
     9421 cgggcccgaa gggctcgccc ttcgaggagt ttttcgacga tttcttcgac gatcagggcg
     9481 gcgacggtct gtcgcgcaag gtcagctcgc tcggctcggg cttcgtcatc gatccggccg
     9541 ggctcgtggt caccaacaac cacgtcatcg agggcgccga cgagatcctc gtcaacttca
     9601 ccgacggcac gagcctgaaa gtgacaaaga tcttgggcca cgaccccaag accgaccttg
     9661 cgctcctcca ggtcgagccg aagaaacccc tcacggcagt caccttcggc gattccgcca
     9721 agatgcgcgt cggggattgg gtgatggcca tcggcaaccc cttcggcctc ggcggcagcg
     9781 tgaccgtcgg catcatctcc gcgaccaggc gcgatatcaa tgccggaccc tacgacgact
     9841 tttttcaaac tgacgccgcc atcaatcgcg gcaattccgg cggtcccctc ttcaacatgg
     9901 agggcgaggt gatcggcgtg aacacggcca tcatctcgcc ttccggcggc tcgatcggca
     9961 tcggttttgc cgtcccctcc aacagcgccg tccaggtgct tgatcaactc aagcagttcg
    10021 gcgagacgcg gcgcggctgg cttggcgtgc atgtccagaa cgtcacggac gagatcgccg
    10081 caagcttggg actggagcaa gccaagggag ctctcgtcgc caaagtgtcg cctggaagcc
    10141 ccgcggccac ggccggcatt cagccgagcg acgtgatcgt caaattcgac ggccagccga
    10201 tcgacaacat gcggagcctg ccgcgcgcgg ttgccgccac ggcggtcggc aagagcgtgg
    10261 cggtggagct gctacgcaag ggcgagacca agagcttctc cgtcaccgtc gggcagctgc
    10321 ccgaagacga ggagggtgag gaggtattgc agagcaatgc aaagcccgcg cccgaaccgg
    10381 agcccgattc tttgctcggc ctcacgctcg cgccgctgac cgatgaggtg cgccagcgct
    10441 acggcatcgg caagtcgatc gaaggcgtcg ttgtcgtcga ggtcaagccg aacagcccgg
    10501 cggcgcagaa ggacgtgaag ccgggcgatg tgatcgtcga ggtcacccag gaaaaggtga
    10561 aacaaccgca agacgtgaag atgcggttgc tggcggtgaa aaagtccggc cggcgttcgg
    10621 tgctgctttt gctgtccgac gccaagggcg agctcagatt cgtcgcagtg ccgaccggct
    10681 agacgcaaaa cttcggccgg tcgcgacgtg acccatcgcc ccgccaaaat gagtcgtccc
    10741 aaggccattc tcatcgccgg gccgaccgcg agcggcaagt ccgcggcggc gcttcggctt
    10801 gccgagcgcc tgggcggcac ggttatcaat gccgactcca tgcaggtcta ccgcgagctc
    10861 tgcattctga cggcgcggcc ggtcgcggcg gaactcgcgc acgtgcccca ccttctctac
    10921 ggcacggttc cggcggcaga gacttattcc gtcggccatt ggctcgccgc tgccgctgac
    10981 gccatcaccg aggccgaatc cgcggggagg gtgccgatcc tgaccggcgg aaccggcctt
    11041 tacttcaagg cgcttgtcga agggctcgcg ccggtgcccg acgtgccgcc cgagattcgc
    11101 gcctattggc gcgagcgggc ggccgcgatc ggtcccgggc aactccatcg cgaacttggc
    11161 agccgcgatc ctgccatggc cgcgcgactt acggcgaccg acccgcaacg cctcgtccgc
    11221 gcgctcgaag tcatcgacgc gacctcgatc tcgctcgcgg aatggcaggg tgcgagcgcc
    11281 gcacccgtcc tcgggccgga gggggtcgtg aagctcgtga tcgcgcccga acgcgaggcg
    11341 ctttatgcgg cgatcgacgc ccgcttcgac gccatggtcg aagcgggcgc catcggagag
    11401 gtgcggtcgt tgctcgctct tgatctcgac cccgggctcc cggcgatgcg ggcgcatggc
    11461 gtgcgcgagt tggcactcta tctcgccggc cggagcagcc gggctgacgc gatcgcaaag
    11521 gccaagaccg agacgcgccg ctacgccaag aggcagatga cctggctcaa gcgcttcatg
    11581 aaggattggg attgggtgcc cgacgccggc tccgccgtcg acgccgcgcg cgcgcaaatt
    11641 gctacacggt aacctgtgtg ccgatctcga ccaccctgtc ggtcggaagc tggaagtagc
    11701 tcgaggcgtc gtcggcattg tgggcgagga agatgtacac ccggtcctgc catgccggca
    11761 tccccgaccg cggatcaggc ctcaccgagc ggcgcgacag gaagaacgac gtcgacatga
    11821 tgtcgaagtg ccaaccctgc ttgcgcgcga gcgccagcgc cctcgggacg ttgggtcgct
    11881 ccatgaagcc gaaatgcata atcagccgca tgaatcggtc gctgatcggc tccagccgaa
    11941 cgcgaacctg tgggttaacg cgggggatgt cctcgttgac gatggtcagg atcacgttgt
    12001 gttcgtgcag caccttgttg tgcttgaggt tgtgaagcag cgccgtcggg gcgaattcgg
    12061 gatcgctggt gaggaacacg gcggtgccgg gcgcgatatt gggcggccgc tcctcgaggc
    12121 tcttgaccag cggtttgaac ggcacctcga cgcgacgtgt ctttagcgcc aagaggcggg
    12181 tgccgcgccg ccaggtcgtc atgatcaaga tcaggatggc gccgaccaaa acgggcagcc
    12241 aagccccctc gatcagcttc aacaggctcg cgatcacgaa gctcgaatcg gcaaagatga
    12301 gagggaccat caccgccgcc gtggtccaca gccgccattt ccactccttc cagatcacga
    12361 tgaagccgag caggcttgcg gcgagagccg tggtcgcgac cgccagaacg taggccgccg
    12421 ccagctcgct cgatgagcgg aacatgaaca ccagaagcag gaccccggtc agaagcaggt
    12481 aattcacgcg cggcatgtag atttgaccgg caagcgcttc cgaggtgtgg cgaacctcca
    12541 tgcgcggcaa aaagccgagt tgcatggcct ggcgggtgat ggagtaggcg cccgagatca
    12601 ccgcttgact ggcgatcgcg gtggccaggg tggcgagcgc aaccattgga tagagcgccc
    12661 agtcgggata gagcagatag aacgggttct cgatcgtctc cggccgctca aggagcagtg
    12721 cgccttggcc gaaatagttg aagagaaggg ccggcagcac gaaatagagc cacgccgtct
    12781 ggatcggctt ccggccgaaa tggccgagat cgttgtagag cgcctccgaa ccggtggcca
    12841 cgaggaacac ggcgccgagc gtgaccagcc cgatgattcc gtgagtgaag aggaaattga
    12901 gaccgtagat cggattgagc gcggagagca cggctgggtt ggcggcgatc tcgacggccc
    12961 ccgcgacggc aatgaccaag aaccagaccg tagtgatagg agcaaacaag gtcgagacac
    13021 gggctgtgcc gtgcgactgc accgagaaca acgcgacgag gatcgccacc gtgatcggca
    13081 gcacgtaggg cccaaatgcc gccgtcgcca cctgcagacc ttcgacggct gacagcaccg
    13141 acagtgccgg ggtgatcaga gtcgagccgt aaaacatcgc ggccccgacg gtcccgagcg
    13201 cgatcacgag cggcgttcgc cgcccgagcg cgcgaaaggc aagcgccgtc agggccagcg
    13261 tgccgccttc gccgttattg tcggcgcgca atagaatgag aacgtacttg atcgtgacga
    13321 cgacgatgag cgaccagaag atgagcgaca gcacgccgta gatcgccgcg ggcgtcacgg
    13381 tgcccccatc gctgcttgcc gccagcaccg cttcgcgcaa ggcgtagagg gggctcgtgc
    13441 cgatgtcgcc gtagacgacg ccgatcgagc cgaaagtcag cgcccagaac tgctttctga
    13501 cgtcggctag gtcaccgttg gcacccgcca gtgcctgcgt ttcagagttg agccgcgcca
    13561 tgcttcctca gacggccact tgggtcccaa tctcgaccac gcggtcagtg ggaagctgga
    13621 aatagctcga cgcgtcgtcg gcgtttcgcg ccagaaggat aaacaagcga tcctgccata
    13681 cgggcatccc ggaacgcggg tccgggcgaa ccgaccgtcg cgacaggaag aacgatgtcg
    13741 acatgatatc gaagcttaga ccttccttgc gcgctagccc aagtgccttc ggcacgttcg
    13801 gcgtctccat gaaaccgaaa cgcaagagca aacgctgaaa cgagccgccg aggggctcga
    13861 tacttacgcg gtcagcgact gaaacgcgcg gggtggtctc cgtgatcatg gtgagaatca
    13921 cgttgcggtc gtgcaacgcc ttgtagtgct tgagactgtg gagcagggca gtcggagcgc
    13981 tctccggatc tgcggtgaga aacaccgcgg tcccggcaac gcggcagggt tcttttttct
    14041 cgagactttg gatcaggctg tcgatgggcg tttccaggcg tcgcgtcttc tcggacagga
    14101 ttcgcgtccc tttgcgccat gtcagcatga cggccatgag catggctgcg atcaggatcg
    14161 ggacccagcc gccatgcatg actttcaaca ggttggcgcc gagaaaaacg aggtcgatga
    14221 ggagaaaagg aatcatgagc gccgctgccg cccatagcgg ccaagcccat actctccaaa
    14281 ccacgatgaa agccaatatc gccgtcacga ccatggttcc cgtcacggcg atgccgtacg
    14341 ccgtagcaag cgcgcccgaa gatttgaaca gaatgacgag aagaagaacg ccgagcagca
    14401 ggatagtgtt cacgcgcggc atatagattt gcccgaactg cgcctccgag gtatggcgga
    14461 tctcgagccg cggcaacagg ccgagttgga ttgcctgctg cgtcagcgag tacgctccgg
    14521 tgatcacggc ctgactggca atgacggttg cggcggtcgc aagtccgatc atgggcacca
    14581 gcgcccactc cggataaagc aaaaagaacg gattctcgat cgtttccggc ctgcccagta
    14641 gcaaggcacc ctgaccgaga tagttgaccg tgagcgccgg cagggcccac ggcaaaccag
    14701 gccgtgcgaa tcggtcctcg accgaaatga ccgagatcgg cgtaaagggc ctcggcgccg
    14761 gtcacggcga ggaaaaccgc cccaagcgtc aacagcccga cgtaaccgtg gccgaggagg
    14821 aaactcgccc cgtaaagagg attgaatgca cgcagcaccg taggattctg cgacacatgc
    14881 cacgcgcccg cggtgccgag cgcaagaaac caggccaggg taatcggccc gaagagcgcc
    14941 gcgacccgtg ccgtcccacg cgattgcacc gcgaacaagg cgaaaaggat cgcaacggac
    15001 aggggaacga cgtagtcctc caaagccggt tccgcgacct tgaggccctc gacggcagac
    15061 agcacggaaa gtgccggcgt aagcatcgca tcgccgtaaa acagtgcggc gctgatcatg
    15121 ccgagcataa ccaccgcgag accgcgtccg cgaaacgtgc gttgagcgag ggccatcaag
    15181 gcgagtgtcc cgccttcgcc gttattgtcg gcacgcagca gaatgatcac atacttgatg
    15241 ctgacgatca ggatcagcgc ccacacgatc agggacagga tgccgagaat gatgtcttcg
    15301 cccgccgggg cccttgcacc gctcgcggca aggaccgctt cgcgcatggc gtagagagga
    15361 ctcgtgccga tgtcgccgta gacgacgccg atcgcgccca aggtcagcgc caggaacgcc
    15421 acttgcgagg acgatggttt gtctgcgctc ccggcggcag aaatgtctgt gcgagcggcc
    15481 tgctgcattt cagcggagaa tgggaatggc gtgaaagata tcgcgactaa cgccgcaagc
    15541 agcggccgcg gggtccctga agcggtaggg agataggcgc aacggcggcg cttgtccagc
    15601 gacggcggcg ggaaaccagg ggctgcggcg ttttggtggg tagcgcggct acgcaagaaa
    15661 agtcatgcat tgagggcagg aaagaaattt ctgaaaataa aaacgtcgca aatattgtca
    15721 ttcattcgca tttttcgttg accctctgga acccaagccc taggttgcgc gccttgttta
    15781 gacttttccg ctattcctcg agctttgacc ggccgcggac cttgaggcgc gaggccggtt
    15841 tttgtcagtg agatctcaaa tgtcgcgcgc aatgactgga gcggagatgg tgcttcaagc
    15901 tctcgccgat cagggcgtgg agcatatctt cggctacccg ggtggcgccg tgctgccgat
    15961 ctacgaccac ctgtttcagc aggaccgtct caagcacatc ctcgttcgcc acgagcaggg
    16021 cgccgtgcac gcggccgagg gctatgcccg ctcgacggga aaggtcggcg tggtgcttgt
    16081 cacctcgggt cccggcgcca ccaacgccgt caccgggttg accgacgcgc tcatggattc
    16141 gatcccgatc gtctgcatca ccggccaggt gccgacccat ctcatcggca acgacgcctt
    16201 tcaggaatgc gatacggtcg gcatcacccg cccctgcacc aagcacaatt atctggtcaa
    16261 ggacgtcaac gctctctccc gcgtcctgca cgaagccttc tacgtcgcgc gcaccggccg
    16321 tcccgggccg gtcgtggtcg atattccgaa ggacattcaa ttcgcgcgcg gcgagtacac
    16381 cgggctcacc aattcgcagc acaagagcta caacccgcgc gtcaaaggcg acgaacgcgc
    16441 ggtcgcgcaa gcgatcgagc tgatggcacg cgccaagagg ccgctcttct ataccggcgg
    16501 cggcatcatc aattccgggc cgctcgccag cgcccggctc cgcgagctcg tccgcatgac
    16561 cggctttccc atcacctcga ccttgatggg actcggcgct ttcccggcct ccgacccgcg
    16621 ctggctcggc atgctcggca tgcacggcac ctatgaggcc aacaacgcca tgcacgattg
    16681 cgacctgatg gtctgcatcg gcgcacgctt cgacgaccgc atcaccggcc ggatcgacgc
    16741 cttctcgccc gggtccacga agattcatgt cgatatcgac cgttcctcga tcaacaagaa
    16801 cgtcaaggtc gacctcccca tcgtcggcga ctgcgcccat gtgctcgagc agatgatcga
    16861 tgcctggaag gccaagaagc ccaagctcga caaggcggcg ctcaaggtct ggtgggccga
    16921 gatcgaccgc tggcgcgcgc ggaactgctt gaaatatcgc aagcgcgatg acgtgatcat
    16981 gccgcaatac gccatcgagc ggctttacgc gctgacccgc gaccaggatg tctacatcac
    17041 gacggaggtc ggtcagcatc agatgtgggc ggcgcagttc ttcaagttcg aggagccgaa
    17101 ccgctggatg acgtcgggag ggctcggcac gatgggctat ggcctgccgt ccgcggtcgg
    17161 cgtgcaggtg gcgcatcccg gttcgctcgt catcgatatc gccggcgagg cctcggtgct
    17221 gatgaccatg caggaaatgt ccacggccgc gcagtacgac ctgccgatca agatcttcat
    17281 cttgaacaac gagtacatgg gcatggtgcg ccaatggcag gaattgctgc atggcggccg
    17341 ctactcccac tcctataccg cggcgctgcc cgatttcgtc aagctcgccg aggcctatca
    17401 aggggtcggc atcagggccg agaagccgtc ggagctcgat gacgcgatca gggagatgat
    17461 ccgggtgaag aagcccgtga tcttcgactg ccgggtcgac aagcatgcca attgcttccc
    17521 catgatcccg agcggggagg cgcacaacaa catgctgatg ggcgacgacg tgaccgaaga
    17581 ggacatcgag aaagccatca ccgaagaagg caaggtgatg gtttagcgcc gagggtcaag
    17641 accttgatac gattcctgct tagcctccgg gccatcatgc tgatcggctc ggccggggcc
    17701 atgatcggcg cgcttctgat gttctttcag ggcggcttct acctttacga ggcgtggcat
    17761 acgctgatga cgccgggcca cgccgccgag cggcaggtca ccgtgccggt gctggaggcc
    17821 gtcgatgcct tcctgttcgg catcgtgctg gtgatcttcg cttacggcat cgccatcggc
    17881 ttcgtcttca ccttgccgga aggctatggt gcgcggctgc ccgagtggat gaaggtgggc
    17941 ggcgtcggcc agctcaaggc caccctcgcc gaagtcgtca tcgtcgtgct gatcgtgatc
    18001 ttcgcccgcg tggtggtcga ggccaacggc cacttccaat ggtcgatact cgtcctccca
    18061 gtctcgatct tctttattgc gcttgcgctg cgcatgatcg agcttggcgg cggcaagggc
    18121 ggaggcgagg ggaaggccgc gcactgacgc gttcatgcca tgaccggaaa tccgacgaaa
    18181 cagccggggt ccgcctatcc gctggagaag aaggaggagc ggctcgaaac ccacaccatg
    18241 accgtgctcg tcgacaatga gccgggcgtg cttgcgcgcg tcatcggact gttcgcaggc
    18301 cgcggctaca acatcgagtc gctgaccgtg tcggagaccg agcatgaggc gcatttgagc
    18361 cgcatcacca tcatcaccac cggcacgccg atggtgatcg agcagatcca gcatcagctc
    18421 gagcgcctgg tgccggtgca ttccgtcacc gacttgacgg tcgagggcaa tccgctcgaa
    18481 cgcgagctcg ccatggtcaa ggtcgccggc aagggcgaga agcgcgtcga ggccctgcgg
    18541 ctcgccgaag cgttccgcgc cgacgtgatc gacacctccg gcgaacattt cgttttccag
    18601 gtcaccggcg agcagtcgaa gatcgaagaa ttcatcaccc tgatgcagcc gctcggtctg
    18661 gtcgaggtgg cacgcaccgg gctcgccgcg atctcgcgcg ggcccgagcg catgtgacgc
    18721 ccgcccgatt tggcaggagg caatgagacc gggcccgaga cccccacttg caaagtccgg
    18781 gcctttcgcg tcacaaacgc ggccgaacga tggagcctaa ctcaagacaa gggacgactc
    18841 gtatgcgcgt ctattacgat cgggatgccg atctcaatct cgtcaagggc aagaaagtcg
    18901 ccatcatcgg ctacggcagc cagggccacg cgcacgcgct gaacctcaag gactcgggcg
    18961 tgaaggacgt ggccgtgggc ttgagacccg gctcgcaatc cgtcgccaag gccgagaagg
    19021 ccgggctcac ggtcatggac gtccccgacg ccggcaaatg ggccgaagtg gtgatgatgc
    19081 tcacccccga cgagcttcag gccggcatct gggagaggga gctgaagccg aacatgcagc
    19141 agggcgcggc gctgttcttc gcccacggcc tcaatattca tttccgtctg atcgagcccc
    19201 gcgccgacat ggatgtatgc atggtggcgc cgaagggccc cggccacacc gtgcgcgccg
    19261 agtatctgcg cggcgcgggc gtgccttgcc tcatggcgat cgcccaggac gcgaccggca
    19321 acgcccatga cctcacgctg tcctacgccg ccgccatcgg cggcgggcgc gccggcatca
    19381 tcgaaaccac gttccgcgag gaatgcgaga ccgatctctt cggcgagcaa tcggtcctat
    19441 gcggcggcct ggtcgagctg atgcggaacg gttacgagac cctggtcgag gccggctact
    19501 cgcccgagat ggcctatttc gaatgcgtgc atgaggtgaa actgatcgtc gacctcatct
    19561 atgagggcgg catcgccaac atgaactact cggtgtcgaa cacggccgag tatggcgaat
    19621 acgtttccgg cccgcgtgtc gtcggtccgg cgagccggga ggccatgaag gagattctcg
    19681 ccgacatcca atcgggaaaa ttcgtcaagg actggatgct ggagaaccgc gtcagccaga
    19741 cgtctttcaa ggcgatgcgc gccaaatgcg acaaccacca gatggaagaa gtgggcaagc
    19801 ggctccgcgc gatgatgccg tggatcgccg agcgcaagat ggtcgacaag agcaagaact
    19861 gacgcctgct ccgatgatgt ctccgtcatg gccgggctga taccgccgca aattagtttc
    19921 cgttcaggac cgcacgtctc actgggctac aggccgccgg cacccgaagt gttcgtgccc
    19981 gcacttgccg cgtggccgtc tgcgcgctgt cccctggcac aaacaccctc taaaccaaca
    20041 ttgcaaatgg accactcgtt ggagggcggg ccacgttcat cgccgcggcc tgcaagtcag
    20101 ctagtcacct ggaagaggac aagcgccgcg tgtcagaagc tgaaatatgc gaaatcctca
    20161 tccgcggagg gaagcacagc ttccctgaag agttcccagc atgccagtgc gacttgaaat
    20221 cgtcatggtg actatgataa ttcgtactca aacggatagt acgtgctcaa acggcgctac
    20281 tgctcgtcgg aatagcgtct gatgtgtgct tatgttgtgg ggagccttta atggaactct
    20341 cccgtagagc aatacttggc accgctgcct gtcttggtct ttcgagcaag cttttcccgt
    20401 ttttcgaggc tgcagttggc gctgaatcca tacgcacgcg gcagaacatc aaagccttcg
    20461 ctgcggaccc gggcaaggtg agcgcgctgc gagccgctgt cggtcacatg aaggagctgt
    20521 ccaatagcaa taggcacgat ccgtttgggt ggagttattg ggcttcgagt catggcagtc
    20581 ccgatcctcc gagcacacaa gagcccaaag atcccaacgc gatgcccctg tacaggcagt
    20641 gcaaaaacat ggcggaaatg gcctgtggga gccgcatttc ctttcttggc accgggtatt
    20701 tctatttttt ttcgaggccg ttctcaagca agcggctgtt gagtctcgcg aaacgacggc
    20761 gttcgacctt ccatactgga actggtacga ggatcccctt cctcagatct tcaaggaagg
    20821 cgacgaaaaa acgaatcctc tatacgtgag aggatcgcta gccggaggtt ccgtcgatct
    20881 agacaaatta ataccaggtc tctcgacggc ccccttcctt agcgacgact ttcttattgc
    20941 tccgggtgtg ccaccagaga atacgttcct ttaccagctc gacgagaatc cgcatggcac
    21001 agtgcatgat ctggttgcgg gggatatggg gcgtgtgcca actgcggcac gtgacccaat
    21061 cttctggttt caccacgtca atctcgatcg gctgtggaca gcttggatga aatctggctc
    21121 gcgccgcctg cctgcccggg attccgcatg gggacgcaca tcgtggttct tcgatccaag
    21181 cggcaactgg aaagccaccg caggcgacat gctcaattct gagacgtcgt tcggatatcg
    21241 atatgacgat gagacccttc cttcggtgtc ggttgcggtc gcttctgcac tgccatcacg
    21301 tacacttgaa gccctacccc tgtcagccgc catggcgcca cccatggatg aagccgcgcc
    21361 ttcgagcact gcgactacgc tcagccaaac ggca
//
LOCUS       DASUQP010000268         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_679001,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000268 DASUQP010000000
VERSION     DASUQP010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679001"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..810)
                     /locus_tag="VFY74_12110"
     CDS             complement(310..810)
                     /locus_tag="VFY74_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932470.1"
                     /translation="MASRARYRLLLPAACCAILGAALVTAAQGRDPAAPYLGMTKDEI
                     IACAGEPHARFRSGAEAETLTYRYSGAGPVPAEPGKSGEKKKDKKKSIADIFGDKKGK
                     KTDKDWTCSASLKFEQGRLVSVYFAHKDARSPYAWQSEDDPKKQEAMRKEVLPTCTFS
                     LPRCTR"
     gene            complement(867..2258)
                     /locus_tag="VFY74_12115"
     CDS             complement(867..2258)
                     /locus_tag="VFY74_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5932471.1"
                     /translation="MLSPVKALAGESPPESAVQRAAAHDRGWRRPSGTPSLSEVFGSI
                     RTRPTGTLWRKLLAFLGPGYLVAVGYMDPGNWATSLAGGSKFGYALLTIALLSNLMAI
                     LLQALCIRLGIASKRDLAQACRDAFPRFVSYPLWLLAELAICATDLAEVIGTAIGLNL
                     LFGIPLEIGVLLTAADVFLILWMQQLGFRYVEALVVTLLVVIAVCFGIQIAMADPNWG
                     EVIRGFAPTVEIVKNPEMLYLALGILGATVMPHNLYLHSGIVQTRRFGESVPEKREAI
                     KLATIDSTIALMFALLINASILILAASTFHRTGQTDVAELGEVYKFLAPLLGSAVAPS
                     LFALALLACGLNSTVTATLSGQIVMEGFLDIRLPPYLRRLLTRAIAIVPAAIVTIWYG
                     AEGTAKLLILSQVVLSLQLPFAIVPLVMFTADRRKMGALVAPIWVTALAVLIAITVIT
                     LNLKLLFDFFTGL"
BASE COUNT          496 a          791 c          870 g          419 t
ORIGIN      
        1 ttctggcggg tgaggtcgaa gctgcaaacg atgtacgact ccgcgccgag actgggtgcg
       61 gcacggctcg gcgctggcgg attgaggcac cgcaagcaag ccggttgcgc aaagcactag
      121 cgggaaattc cgccagcgcg gaaacggtcg ccgaaccggg cgcgcatggg tgcgccttcg
      181 gggagtgcga tgcggcggcc gcgacgcccc gcaagtgcga cgcgggtcag cttttaccgg
      241 ttggctgtca gccattgcgc gggcctgcga cggcggaggc tcgcatcaag cacgactcgc
      301 gcaagaacgc tagcgggtgc agcgcggcaa ggagaaggtg caggtcggca gcacttcctt
      361 ccgcatcgcc tcctgctttt tcgggtcgtc ctcgctctgc caggcatagg gactgcgcgc
      421 gtccttgtgc gcgaaataca cgctgacgag ccggccttgt tcgaatttga ggctcgccga
      481 gcaggtccaa tccttgtccg tcttcttgcc cttcttgtcg ccgaagatgt cggcgatcga
      541 ctttttcttg tcctttttct tctcgccgga cttgccgggt tcggccggca cgggccccgc
      601 gccgctatag cgataggtga gcgtctcggc ctcggccccg ctccgaaagc gtgcgtgagg
      661 ctcgccggcg cacgcgatga tttcgtcctt ggtcatgccg agatagggcg cggctggatc
      721 gcgaccctga gctgcggtga caagagccgc gccgagaatg gcgcaacagg ccgccggcag
      781 aagaaggcgg tagcgcgctc gtgaagccat gcgggggcgc atgacattgc tcgtcgcccc
      841 caaatccggc ggcatcatgg cggcgcctag agcccggtga agaagtcgaa cagcagcttc
      901 aggttcaagg tgatcaccgt gatggcgatc agcaccgcaa gcgccgtcac ccagatgggt
      961 gcgaccagcg cccccatctt gcgcctgtcc gcggtgaaca tcaccagcgg cacgatggcg
     1021 aacggcagct ggaggctcaa caccacctgg ctgaggatga gcagcttcgc cgtgccctcg
     1081 gctccatacc agatggtgac gatggcggca ggcacgatgg cgatggcgcg ggtgagcagc
     1141 cgcctgagat aaggcggcag gcgaatgtcg aggaaaccct ccatcacgat ctggcctgac
     1201 agcgtcgccg tgaccgtcga gttgaggccg caagcgagca gcgccaatgc gaacagagac
     1261 ggcgccaccg ccgagccgag cagcggcgcc aggaacttgt agacctcgcc gagctcggcc
     1321 acgtcggtct gcccggtgcg gtggaaggtg gaggcggcca ggatcaggat cgaagcattg
     1381 atgagcagcg cgaacatgag cgcgatggtc gaatcgatgg tggcgagctt gatcgcctcg
     1441 cgcttctccg gcacgctttc gccgaatcgc cgcgtctgga cgatgcccga atgcagatag
     1501 agattgtgcg gcatcaccgt ggcgccgaga atgccgagcg cgagatagag catctccgga
     1561 ttcttgacga tttcgacggt cggcgcgaac ccgcggatga cctcgcccca attggggtcg
     1621 gccatggcga tctgaatgcc gaagcagacg gcgatgacca ccaacagcgt gaccacgaga
     1681 gcttcgacat agcggaagcc gagctgctgc atccacagga taagaaagac gtcggccgcg
     1741 gtgagcagga cgccgatctc gagcggaatg ccgaacaaga gattgagccc gatcgccgtg
     1801 ccgatcactt cggcgagatc ggtggcgcag atggcaagct cagcgagcag ccatagcgga
     1861 taggacacga agcgtggaaa ggcgtcgcgg caggcttgcg ccagatcgcg cttcgacgcg
     1921 atgccgagcc tgatgcagag cgcctgcaac aggatcgcca tcaggttgga gagaagcgcg
     1981 atggtgagga gcgcgtagcc gaatttcgac ccgccggcga gagaggtcgc ccaattgccg
     2041 ggatccatgt aacccacggc gacgagatag ccgggaccga ggaaggcaag gagcttgcgc
     2101 catagcgtgc cggtcggccg cgtcctgatc gagccgaaca cttcgctgag cgacggcgtg
     2161 cccgaaggcc gccgccagcc gcgatcatgc gcagcggccc tctggacggc gctctcgggc
     2221 gggctctcgc ctgccagcgc tttgaccgga ctcagcatcg ctcagacctt ttcatctccg
     2281 tcatcccaag agcccggcac gcgcctcgaa ggacgcgcgt tcgggctcga ccagcccaac
     2341 ggcttggccc ggcactgact tcccacttgg cggataccct ctggcgggac tgtcagcctg
     2401 ttatctagcg taaatgagaa tgatttgcaa ctgcaagagg gatgcagccg ccccaaaccg
     2461 ggcggaacca aacgggccca gaagactggg aacgaggtca attgtcgaag gatctgttgc
     2521 cacgttgatt gttggtggcg gcgccgtgtt gacggcgatc gctgtatacc tcgcca
//
LOCUS       DASUQP010000269        13850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_2041818, whole genome shotgun sequence.
ACCESSION   DASUQP010000269 DASUQP010000000
VERSION     DASUQP010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13850
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041818"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFY74_12120"
     CDS             <1..450
                     /locus_tag="VFY74_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5932472.1"
                     /translation="GLILAPATHEIFTAERGKGAFLNDRRIRVAARTEIADTVIGTGI
                     PHSGRPAHDLYLSELRAVMATAAGVRRLGAASLDLAWTAAGRLDGFWERNLRAWDLAA
                     GIVILREAGGFVSDAEGKDAMLAQGSVVAGNEAIQRKLLKLLNTAGS"
     gene            794..1822
                     /locus_tag="VFY74_12125"
     CDS             794..1822
                     /locus_tag="VFY74_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HEX5932473.1"
                     /translation="MAPVTYKVKLTRPQVFLWRMTLFLILAILLAIILDGQTGQLRKS
                     FMANPGLNGLIIGVLVVGIVYAFRQVLRLYPEINWVNNFRISDPGLTVEKSPVLLAPM
                     ATMLRDRTGQLSLATGAMRSLLDSVASRLEEQRETTRYLVGLLIFLGLLGTFWGLLET
                     VSTVGNTISTLDTNAGESVLLFDQLKEGLAGPLRGMGTAFSCSMFGLAGSLILGFLDL
                     QAGHAQGRFYNELEDWLSGITELQLAEAPGAAGMAPQVRFALLDMQRSLADLNEKVEL
                     KLLNDNTGDAVKDLAAGVDKLIRQMRAEQKVVREWADEQAQQQQELATVLRQITDKAK
                     LPAREERK"
     gene            1825..2853
                     /locus_tag="VFY74_12130"
     CDS             1825..2853
                     /locus_tag="VFY74_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HEX5932474.1"
                     /translation="MSLARARRGRFQSNYWPGFVDMLSTLLLVVTFLMALFMLANFVV
                     TQAASGKDTMLTKLNRQLAELTELLALERSKKESVEDNLVALQATLSDKDEENERLTG
                     LLGAASGQTQGAEDRVRSLGASLEEQKKITNDALAKVEMLNQQLAALRLQLASLQEAL
                     EASEAKDRESQAKIADLGQRLNVALAKKVQELARYRSDFFGKLKEALGNRPDFQVVGD
                     RFVFPSDVLFDTGSSALRPEATYQLDKLGTALHELETKIPADIAWVMRIDGHTDIRPI
                     ATPEFPSNWELSSARAISVVRYLMDQGVPANRLVAAGFGEFQPMDAGESEEALSKNRR
                     IELKLTDR"
     gene            complement(2869..4704)
                     /locus_tag="VFY74_12135"
     CDS             complement(2869..4704)
                     /locus_tag="VFY74_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5932475.1"
                     /translation="MQFREQQPEGRARDRERAQQSAEKEEAKHTSLRSLGALKPYLFR
                     HKAMLAAALIALLVSAGATLAVPLAVRRMIDLGFSGVDPNLIDKYFITLAGIGLVLAA
                     ASAARFYAVNWLGERVVADIRRDVFAHLTGLSPAFFEVSHSGEIMSRITADTTQVKAA
                     ASTAISQALRNILLLIGSVIMMIVTSAKLSLAVLIAIPLIVLPLFAYGRSVRKLSREA
                     QDTLAQASAYASESLSQVRVMQAFTHEKTAAGRFGVAVERSFAAANARAKARAGLTAI
                     AIFLTFASVVGVLWYGAQDVLSGAMTGGRLSQFVLYAVLAASAVGGLSEVWGDVAQSM
                     GAAERLGELLKARSSITSPPNPKAFPVPPRGEIAFRDVSFSYPLRPDTRALDHVSFRV
                     SPGERVALVGPSGAGKTTIFALLLRFYDTDCGKVEVDGLGVNEAALDALRACFALVPQ
                     DTALFADSVAANIAYGAKEASAHEIEAAARAAFAHDFIMALPQGYDTMLGEGGVTLST
                     GQRQRIAIARAVLRNAPILLLDEATSALDAESETLVQRALDKIMEGRTTLVIAHRLAT
                     VMRADRILVLDKGRLVEEGTHQSLVGSGGIYKRLAELQFVPDAAE"
     gene            4886..5107
                     /gene="rpmE"
                     /locus_tag="VFY74_12140"
     CDS             4886..5107
                     /gene="rpmE"
                     /locus_tag="VFY74_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX5932476.1"
                     /translation="MKKDIHPNYHRIKVVMTDGTEFVTYSTYGAEGDTLKLDIDPKTH
                     PAWTGGGQHLIDRGGRLTRFKRKYEGFLK"
     gene            complement(5130..5660)
                     /locus_tag="VFY74_12145"
     CDS             complement(5130..5660)
                     /locus_tag="VFY74_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX5932477.1"
                     /translation="MAGRSKSDGNVTVSFGAKFARSDQFKSVFREGMALVEAAATYLD
                     GDGRKEARKLRPPQSLAYATESMRLTTRLMQLASWLLIRRAVSEGELTPEQAKEEQRK
                     IKLPVIGSEQPPRDLGSLPVRLRELIEQSIKLQERVIRLDQMLGTEYVPQGAAADHPL
                     KSQLSRLRSAFGKDAE"
     gene            complement(5932..6123)
                     /locus_tag="VFY74_12150"
     CDS             complement(5932..6123)
                     /locus_tag="VFY74_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX5932478.1"
                     /translation="MDLDELQPVKKPKGYELGQELSKHSVGELEALIEALKAEIARVE
                     QALHAKQSSKSAAEAAFKR"
     gene            6189..7184
                     /locus_tag="VFY74_12155"
     CDS             6189..7184
                     /locus_tag="VFY74_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX5932479.1"
                     /translation="MPPKTMIAIEAKGTGGPEVLVPGTRPVPEPGPGEVLIKVAASGI
                     NRPDVLQRQGLYPPPKGASDLLGLEVAGTVVKRGPGAVRFNEGDRVCALVNGGGYAEY
                     ALAPEDTTLPVPQGLTFTEAAALPETVFTVWHNIFERAQLKAGEWLLVHGGASGIGTT
                     AIQIGAALGAHVIATVGSAEKAEAALALGASRAVNYGEEDFVEAVRGATGGEGANVIL
                     DMVGGDYIERNLRAAALDGRIVQIAFLKGSKVEIDLMRLMLRRLTLTGSTLRAQTPDA
                     KARIAKGVEQNVWPLIARGKLKPVIDSTFRLDQAAEAHRRIDDPGHIGKIVLEVR"
     gene            7314..8087
                     /locus_tag="VFY74_12160"
     CDS             7314..8087
                     /locus_tag="VFY74_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEX5932480.1"
                     /translation="MDRTPLMPKATAVWLVDNTSLTFEQIAEFCGLHVLEVKGIADGD
                     VAHGIKGMDPISSGQLSREEIAKAEREPSHRLKLSEPKVEVPEVKTKRGPRYTPVSRR
                     QDRPNAILWLLKNHPELKDSQIMRLVGTTKPTIIAIRERSHWNSPNLQAQDPVTLGLC
                     SQTDLDAEVKKAAARIERERKQAGLAEPPKAGTLLPTEESTAAPPEPEPQPEQPMATA
                     LAQEARTAMAAPDEEEETPDIESVFAKLKDLKRNDAPSE"
     gene            8177..9802
                     /locus_tag="VFY74_12165"
     CDS             8177..9802
                     /locus_tag="VFY74_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5932481.1"
                     /translation="MANPYETGLDKTPANYQPLTPLVFLQRAAAVHPGHAAIIHGKAQ
                     TSYADFYARARKLASALAAHGIGLGDTVSVMLANTPAMLEVHYGVPMVEAVLHAINTR
                     LDASAVAFMLDHADTKVLIGDTEFAGVVAEALKQVKVKPLVIDYRDLECGVEGTRLGT
                     LDYEDFVASGDPQFPWVMPGDEWNAISLNYTSGTTGNPKGVVYHHRGAALMCYANALA
                     TGMGQHPVYLWTLPMFHCNGWCFPWSISLLSGTHVCLRAVRAKPMYDAIADHKVSHLS
                     GAPFVMSALLNATDDERRLFPQRVAFSHAAAPPPAAVLARMDEAGFELTHLYGLTETY
                     GPATLNEWHAAWDELPPDERLDKRIRQGVRYHALEGLAVMNPETMEEVEDDGETLGEV
                     MFRGNIVMKGYLNNKKATEEAFAGGWFHSGDLGVIHPDGYIQLKDRSKDIIISGGENI
                     SSIEVEDVLYKHPAVAAAAVVAKPDTKWGETPCAFVETKAGAKVTEDEIIAWCKEKLA
                     RYKCPRHVVFIELPKTSTGKIQKFKLRDMAKAV"
     gene            complement(9832..10149)
                     /locus_tag="VFY74_12170"
     CDS             complement(9832..10149)
                     /locus_tag="VFY74_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX5932482.1"
                     /translation="MVLAVHPGRLLKRELQARGLSANRLALDLGVPSGRITDILNGRR
                     SITADTALRLGRYFGNRAQFWLDLQSQHDIALIESERAMKSRSACARPMPLDGDRTSD
                     RQA"
     gene            10448..10687
                     /locus_tag="VFY74_12175"
     CDS             10448..10687
                     /locus_tag="VFY74_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEX5932483.1"
                     /translation="MIGREIWLKFATWILLALEFALAADLVRTAVAPTWDDISKLAVI
                     ATIRTMLNYFLAKDIAEFDRARQTGGNPPSGRVAS"
     gene            10885..12789
                     /locus_tag="VFY74_12180"
     CDS             10885..12789
                     /locus_tag="VFY74_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5932484.1"
                     /translation="MISKTSTSGPRFQLILIKPSHYDNEGYVVQWLRSTMPSNSLAAV
                     FSLARGAAERQLLGPDLPIDVMAMDETNTRVRPREIARRIAENGGKGLVGIVGAQSNE
                     FPRAVDIARPLRAAGIQVLIGGFHVSGCLAMLKELPEDIKEAQRLGVSIYAGEAEDGF
                     DEAILDAARGQLRPLYDHMKHLPDIGEVASPPFLPVDFVRRTIGNVTSFDAGRGCPFQ
                     CSFCTIINVQGRKSRYRSPDSVEQIIRMNYEQGVNRFFITDDNFARNKDWEAIYDRII
                     KLREEDGLDIRFCIQVDTLCHRIPNFIDKSRRAGVTRVFIGLENINPANLIAAKKRQN
                     KITEYRKMLLEWKRVGIITYAGYILGFENDTPESIRHDLEIIKKELPLDMLEFFILTP
                     LPGSEDHKVLWEKGVWMDPDMNKYDVEHVVTAHPKMSAAEWKEAYRTAWGHYYTDDHL
                     ETIVRRAYASGINIRSLMPVLFWFSSAVPVENLHPLQWGIFRVKYRHDRRSGLPLESA
                     LVFYSRYAADIARKVVVVARRWRHLKRIVRKIEADPRAKLYMDEALTPVTEEDSEHME
                     LYTQNEAARSAVERELRVARKSANGHGRKAPVTSDQPGNGRQPGGRDAGPSSAAPSLS
                     DQSEPISPIV"
     gene            complement(12866..13660)
                     /locus_tag="VFY74_12185"
     CDS             complement(12866..13660)
                     /locus_tag="VFY74_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932485.1"
                     /translation="MALYMVVSPAPATKRVGSEPSSRTLAYEEKIARTPEETKPAVAQ
                     EQAATPKASVPAATAPAQGPEAAPAPAAGANVPASEQSETEMAALPPDDGEASDPNAL
                     PWQHQGPPNAPPGMEAEGTDPMPPPPGAYDDPSDPAAALREDVDPNAWPGGPEEEPQE
                     WVQVLVSGAGMHGTAAEDAPMLFAFPYGRVLRVVSRYEGWVEVTDPQSAATGWMQAHL
                     LAPAPPPGTPYDAEAMYEEEPRGRRGWWRRNGDAFGDMINRALGGL"
BASE COUNT         2562 a         4544 c         4339 g         2405 t
ORIGIN      
        1 gggctgatcc tcgccccggc gacgcacgag atcttcaccg ccgagagggg caagggcgcc
       61 tttctcaatg atcgccgcat cagggtcgcg gcgcggaccg agattgcaga caccgtgatc
      121 ggcaccggca ttccccatag cgggcggccc gcgcacgatc tctatctttc cgagcttcgc
      181 gccgtgatgg cgacggcagc cggcgtgcgg cggctcggcg ccgcctcgct cgacctcgcc
      241 tggaccgcgg caggccggct cgacggcttc tgggaacgaa acttgcgcgc ctgggacctt
      301 gcggcgggga tcgtcatcct gcgcgaggcc ggagggtttg tgagcgatgc cgagggcaag
      361 gatgccatgc tggcgcaagg cagcgtggtc gccggcaacg aagccatcca gcgcaagctg
      421 ctcaagcttc tcaacaccgc cgggtcctga tctgcctgcc ccggtcgagc ggctccgtcg
      481 gcgaccacca accctcccct tgatgggagg gtagcgcgcg atcttccgcg cgccgggtgg
      541 ggtgatggcg ttcaactcgc gaccgcttcg ccctttggaa actcaagccc cccacccggc
      601 acgcaatgcg tgccgacctc cccacgaggg ggaggttcaa aacatggcgt ggctagaaat
      661 ttgcccgctc tccgcttgca cgacccccgg cggaatgcct gcttacgggt ccccgccccg
      721 aggttgccgt tttgccgggt gtagtattcg gttaagcttg aatcgttcga ccgactcgaa
      781 attgcggcag cgcatggcac cggtcactta caaggtcaaa ctcacgcgcc cccaggtctt
      841 tctctggcgc atgaccctgt ttctcattct cgccatcttg cttgcgatca tcctcgacgg
      901 acagacgggg cagcttcgca agagcttcat ggcaaatccc ggcctgaacg ggctcatcat
      961 cggcgtgctc gtggtcggca tcgtttatgc gttccgccaa gtcttgcggc tctaccccga
     1021 gatcaactgg gtgaacaatt tccgcatctc cgatcccgga ctcaccgtcg agaagagccc
     1081 ggtcctgctc gcgccgatgg cgaccatgtt gcgggaccgc accggccagc tgtcgctcgc
     1141 gaccggagcg atgcgctcgc tgctcgattc ggtcgcatcc cgcctcgaag agcagcgcga
     1201 gaccacgcgc tatcttgtcg gcctcctcat ctttcttggc ctgctcggca ccttctgggg
     1261 tttgctcgag acggtgagca cggtcggcaa caccatcagc acgctcgaca ccaatgcggg
     1321 cgaaagcgtg ctgctgttcg atcagttgaa ggagggtctc gccggcccgc tcagaggcat
     1381 gggcaccgcc ttctcgtgct cgatgttcgg gcttgccggt tctctcatcc tcggctttct
     1441 cgatctccag gccggccatg cgcagggccg cttctacaac gagctcgagg attggctctc
     1501 gggcatcacc gagctgcaac tggccgaagc gcccggcgct gccggcatgg cgccgcaggt
     1561 ccgttttgcc cttctcgaca tgcagcgctc gctcgccgac ctgaacgaaa aggtcgaact
     1621 caaattgctg aacgacaaca ccggcgacgc ggtcaaggac ctcgccgcag gggtcgacaa
     1681 gctgatccgg cagatgcgcg ccgagcagaa ggtcgtgcgc gaatgggccg acgagcaggc
     1741 gcagcagcag caggagctcg ccaccgtgct ccgccagatc accgataagg cgaaattgcc
     1801 ggcgcgcgag gagcgcaaat agccatgtcc ttggcgcgcg cccgccgcgg acgtttccag
     1861 tccaattact ggccgggctt cgtcgacatg ctctccaccc tgctcctggt ggtgaccttc
     1921 ctcatggcgc tgttcatgct cgccaatttc gtcgtgaccc aggccgcgag cggcaaggac
     1981 acgatgctga cgaagctgaa ccgccagctc gccgagctga ccgagctgct cgcgcttgaa
     2041 cgttcgaaga aggaatccgt cgaggacaac ctcgtggcgt tgcaggcgac ccttagcgac
     2101 aaggacgagg aaaacgagcg cctgaccggg ctgctcggcg ccgcgagcgg ccagactcaa
     2161 ggcgcggagg atcgcgtccg ctcgcttggc gcatcgctcg aggagcagaa gaagatcacc
     2221 aatgacgctc tcgccaaggt cgagatgctg aaccagcagc tcgccgcgct ccggctccag
     2281 cttgcctcgc tccaggaagc gctcgaagct tccgaggcca aggacaggga gagccaggcc
     2341 aagatcgccg atctcggcca gcgcctgaac gtggcccttg ccaagaaggt gcaggagctc
     2401 gcgcgctatc gttccgattt cttcggcaag ttgaaggagg cgcttggcaa ccgtccggac
     2461 ttccaggtgg tcggcgatcg cttcgtgttt ccttccgacg tgctgttcga caccgggtcc
     2521 tcggctctcc ggcccgaggc cacgtaccag ctcgacaagc tgggcacagc gttgcacgag
     2581 ctcgagacga agatccccgc cgacatcgcc tgggtcatgc gcatcgacgg ccacaccgat
     2641 atccggccga tcgcgacgcc ggaatttccg tccaactggg aactgtctag cgcgcgcgcg
     2701 atctcggtgg tgcgctatct catggaccag ggggttccgg ccaaccggct tgttgcggcg
     2761 ggcttcggcg agttccagcc gatggatgcg ggcgagagcg aggaagccct ctcaaagaac
     2821 cgccgcatcg aattgaagct gaccgaccgc tagcgcttgc ggccatctct actcggcagc
     2881 gtcgggaacg aattgcagtt cggccagccg tttatagatg ccgccactgc cgacaaggct
     2941 ctgatgcgtt ccctcttcga cgaggcgccc tttgtccaac acgagaatgc ggtcggcgcg
     3001 catcaccgtc gccagccgat gcgcgatcac gagcgtggtg cggccttcca tgatcttgtc
     3061 gagtgcccgt tgcacgagcg tctcgctctc ggcgtcgagc gcgctggtcg cctcgtctag
     3121 gagcaggata ggggcattgc gaagcacggc acgtgctatg gcgatccgct ggcgctgtcc
     3181 cgtcgaaagc gtcaccccgc cctcccccag catggtgtcg tagccttgcg gcaacgccat
     3241 gatgaaatca tgggcgaagg cggcccgagc ggcagcctcg atctcgtggg cgctcgcctc
     3301 cttcgcgcca taagcgatat tggctgcgac actatcggcg aacagcgcgg tgtcctgcgg
     3361 cacgagcgca aagcaggcgc ggagcgcgtc gagcgcggcc tcgttgacgc ctagtccatc
     3421 gacctcgacc ttgccgcaat cggtgtcgta gaagcgaagg aggagcgcga agatcgtggt
     3481 cttgcctgcg cccgacggcc ccaccagcgc aacgcgctct cccggtgaaa cgcgaaagct
     3541 cacatgatcg agcgcgcgcg tatccggccg caaggggtag gagaaggaca cgtcgcggaa
     3601 ggcgatttct ccacgcggcg gtacggggaa cgccttcgga tttggcggtg aggtgatgct
     3661 cgaccgggct ttcagcagct cgcctaggcg ctctgcggcg cccatcgatt gggccacgtc
     3721 gccccaaact tcgcttaacc ccccgacggc ggacgccgcg agcacggcgt agagcacgaa
     3781 ctgagacagc ctgcctccgg tcatggcccc cgacagcacg tcctgggcgc cataccacag
     3841 cacgccgacg acgctggcaa aggtgaggaa gatggcaatg gccgtcagcc ccgcgcgcgc
     3901 cttcgcccgc gcattggcgg ccgcgaagga tcgttcgacg gcgacgccga agcgccccgc
     3961 agccgtcttc tcatgcgtga aagcctgcat cactctgacc tgggagaggc tctcgctggc
     4021 ataggcggaa gcctgcgcga gcgtgtcctg cgcctcgcgc gaaagctttc tgaccgagcg
     4081 gccataggcg aagagcggca gcacgatgag gggaatggcg atcagcacgg caagcgacaa
     4141 ttttgcgctt gtcacgatca tcatgattac ggagccgatc aggagcagga tgttgcgcaa
     4201 cgcttgcgag attgccgtgc ttgctgccgc cttcacctgg gtggtatcgg ccgtgattcg
     4261 cgacattatc tcgcctgaat gcgaaacctc gaagaaggcg ggacttaagc ccgtcaggtg
     4321 agcgaacaca tcgcggcgaa tatcggcgac cacccgctcg cccagccagt tcaccgcgta
     4381 gaagcgcgcc gcgctcgccg ccgcgagaac gaggccgatg ccggccagcg tgatgaagta
     4441 tttgtcgatg aggttgggat cgaccccgga gaagccgaga tcgatcatgc gcctgacggc
     4501 aaggggcacg gcaagagtgg cgccggccga tacgaggagc gcgatgagcg cggcggcgag
     4561 catcgctttg tgacggaaaa gataaggttt gagcgcgccg agcgagcgaa gcgaagtgtg
     4621 cttcgcctcc tccttctcgg cgctctgttg cgcgcgttcc cggtcgcgcg cgcgaccttc
     4681 cggttgttgc tctcgaaact gcacgaagac tggcccagct tttcgccgtg ggtcagactt
     4741 taagggggga gagcgccggc ggaaagggcc aatgacgcgc cttcgcggcg cagcaccatc
     4801 tttgcggcgc agccttgcgg cctccgtgaa gttccgctat aggctccgct caacgaaagc
     4861 ggcggatttc gcgaaggctt aagccatgaa gaaggacatc catccgaact atcaccgcat
     4921 caaggtggtg atgaccgatg gcaccgagtt cgtcacgtat tcgacctatg gggccgaggg
     4981 cgacacgctc aagcttgaca tcgatccgaa gacgcacccg gcctggactg gcggcggcca
     5041 gcatctcatc gatcgcggcg gacggctcac ccgcttcaag cgcaaatacg aaggcttcct
     5101 taaatagcgc ggcaagctgc cgcagcttcc tattcggcgt ccttgccgaa ggctgagcgc
     5161 agacgcgaaa gctgcgactt gagcgggtgg tcggcagccg cgccttgcgg cacatactcg
     5221 gtgccgagca tctggtcgag gcggatcacc cgctcctgaa gcttgatgct ttgctcgatc
     5281 agttcgcgca agcgcaccgg caacgatccg aggtcgcggg gaggctgctc gctcccgatc
     5341 acaggcagct tgatcttgcg ctgctcctct ttggcctgct ccggcgtcag ctcgccctcg
     5401 cttacggcgc ggcggatcag gagccacgag gcaagctgca ttaggcgggt ggtgagccgc
     5461 atgctttccg tggcgtaggc gagcgattgc gggggccgga gcttgcgcgc ttctttccgt
     5521 ccatcgccgt cgagataggt cgcagccgcc tcgaccagcg ccatgccttc gcgaaaaacc
     5581 gatttaaatt gatcggagcg tgcgaacttt gcgccgaacg agaccgtaac gttaccgtcg
     5641 cttttcgatc tccccgccat tgcccaactc ccgtctacgc acgagccccg gcggcataat
     5701 atgccatgcg gaggaaggcg cgtcgccctt ccagcctcca aggttaaaac cggcgaatgg
     5761 ggcaaaaaaa gagccgccat gcggcggctc aggaagttaa cagggaggcg tcaaacagag
     5821 tggaaggcca ctcagatcca gaaggctgga tgaccgagac cttaacggga gaatcctaag
     5881 gacgagttaa tgctaagcgc cgatttacct aaacaaagcg tgggggcgcc ctcatcgctt
     5941 gaacgcggcc tctgcggcgc tctttgagga ctgcttggca tgaagcgcct gctccacgcg
     6001 cgcgatttcg gccttcagcg cctcgatcag cgcttcgagc tcgcccaccg agtgcttgct
     6061 gagttcttgg ccgagctcgt agcccttggg cttcttcact ggctgaagtt cgtcgaggtc
     6121 catgcatcta ttctagcacc ttgcccaaat ctggctaagg tgccgtcccc ttcgaacggg
     6181 agaatgtgat gccgccaaag accatgattg cgatcgaagc caaaggcacg ggcggccccg
     6241 aggtgctcgt tccggggacg cgtcctgtcc cggagcccgg tcccggcgag gtcctgatca
     6301 aggttgcggc gtccggcatc aaccgtcccg acgtcctcca gcgtcagggc ctttaccctc
     6361 cgcccaaggg cgcgtcggac ctcctcgggc tcgaggttgc cggcacggtc gtgaagcgcg
     6421 gtccgggcgc cgtccgcttc aacgaaggcg accgcgtctg cgcgctcgtc aatggcggcg
     6481 gttatgccga atacgctcta gcgcccgaag acaccactct gccggtgccg caaggtctta
     6541 ctttcaccga ggcggctgcg ttgcccgaga cggtcttcac cgtctggcac aacattttcg
     6601 agcgggctca gctcaaggca ggcgaatggc tcctggtgca tggcggcgca agcggcatcg
     6661 gcacaaccgc gatccagatc ggagccgcgc ttggcgctca tgtgatcgca acagtgggaa
     6721 gcgccgagaa ggcagaagcg gctctcgcgc tcggcgcgtc acgcgccgtc aattacggcg
     6781 aggaggattt tgtcgaggcc gtgcgtggcg cgaccggtgg cgagggcgcc aatgtcattc
     6841 tcgacatggt gggcggcgac tacatcgagc gcaatctgcg cgcggccgcg ctcgacgggc
     6901 ggatcgtgca gatcgccttc ctcaaaggct cgaaggtcga gatcgacctg atgcggctga
     6961 tgctgcggcg cctcaccctc accggctcga cgcttcgcgc ccagacgccc gatgccaagg
     7021 cccgaattgc gaaaggcgtc gagcaaaacg tctggccgct catcgcccga gggaagctca
     7081 agccggtgat cgactcgacc ttcaggctcg atcaggccgc cgaggctcac cgccggatcg
     7141 atgacccggg ccacatcggc aaaatcgtgc tcgaagtgcg atagaaggcc caaacgacaa
     7201 ttgcttaagt gagagtgaca gcctatatag agtcattccc cttcagtaac ctcgattttc
     7261 cgtctttcgc cgaaggggcg gaaggccgcg ccaatctcaa gttctggaag cccatggacc
     7321 gtacccctct gatgcccaaa gcgaccgccg tctggctggt cgacaacacc tcgctcacct
     7381 tcgagcagat cgccgagttc tgcggcctgc atgtgctcga ggtgaagggc atcgccgacg
     7441 gcgacgtggc ccacggcatc aagggcatgg acccgatctc gtcggggcag ctctcgcgcg
     7501 aggagatcgc caaggccgag cgcgaacctt cgcaccggct caagctgtcg gagcccaagg
     7561 tcgaggtgcc cgaggtgaag accaagcggg ggccgcgcta cacgccggtg tcgcgccgcc
     7621 aggaccggcc gaacgccatt ctctggctct tgaagaacca cccggagctc aaggactcgc
     7681 agatcatgcg cctggtcggc accacgaagc cgacgatcat cgccatccgc gagcggagcc
     7741 actggaactc gcccaatctc caggctcagg acccggtgac actcgggctg tgctctcaga
     7801 ccgacctcga cgccgaggtg aagaaggcgg cggcgcggat cgagcgcgaa cggaagcagg
     7861 caggcctcgc cgagccgccc aaggccggaa ccttgctccc aaccgaagag agcaccgccg
     7921 cgccgcctga gccggagccc cagcccgagc agccaatggc gaccgcactc gcgcaagagg
     7981 ctcgcaccgc catggcggct ccggatgagg aagaggagac gcctgatatc gagtcggtgt
     8041 tcgccaagct gaaagatttg aagcgtaacg acgctccctc ggagtagccc tttccgtcct
     8101 tcccggtttc cgattgccga tcggctcatc ccttcgctag gctgagagca ggaaacgcgg
     8161 ccttctcggg atggcgatgg ccaaccctta cgaaaccggt ctcgacaaga ccccggcgaa
     8221 ctaccagccg ctgaccccgc tcgtgttctt gcagcgggca gccgccgttc atccgggcca
     8281 cgcggcgatc atccacggca aggcgcaaac gtcatacgcg gacttctacg cgcgcgcccg
     8341 caagttggct tccgcgcttg ccgcgcacgg gatcggcctc ggcgacacgg tttctgtcat
     8401 gctcgccaac acccccgcca tgctcgaagt ccattatggc gtgccaatgg tcgaggccgt
     8461 gctccacgcc atcaacacgc gcctcgatgc ctcggccgtc gccttcatgc tcgaccacgc
     8521 cgacaccaag gtcctgatcg gcgacaccga gttcgcaggc gtggtcgccg aagctttgaa
     8581 acaggtcaag gtgaagcccc tggtcatcga ttaccgcgac ctcgaatgcg gggtcgaagg
     8641 cacgaggctc ggcactctcg actacgagga tttcgtcgca agtggcgacc ctcaatttcc
     8701 gtgggtcatg cccggcgatg aatggaatgc gatttcgctc aactatacga gcggcacgac
     8761 cggcaacccc aagggcgtcg tctatcatca tcgcggcgcg gcgctgatgt gctacgccaa
     8821 cgcgctcgcc accggcatgg gccagcaccc ggtctatctg tggacgctgc cgatgttcca
     8881 ctgcaacggc tggtgctttc cctggtcgat ctcgctcctc tccggcaccc atgtctgcct
     8941 gcgcgcggtg cgcgccaagc ccatgtacga cgccatcgcc gaccacaagg tgtcgcatct
     9001 ctccggcgcg cccttcgtca tgtcggccct actcaatgcc accgatgacg agcggcgcct
     9061 ctttccccag cgtgtcgcct tcagccatgc ggcggccccg ccgccggccg ccgtcctggc
     9121 caggatggat gaggccggct tcgagctgac ccatctctac ggcctgaccg agacctacgg
     9181 ccccgcgacg ctcaatgagt ggcatgccgc ctgggatgag ctcccgccgg acgaacggct
     9241 cgacaaacgc atccgccagg gcgtgcgcta tcacgcgctc gaaggtcttg cggtgatgaa
     9301 ccccgagacc atggaagagg tcgaggacga tggcgagacg ctgggcgagg tcatgttccg
     9361 cggcaatatc gtgatgaagg gctatctcaa caacaaaaag gccaccgagg aggctttcgc
     9421 cggcggctgg tttcattccg gcgatctcgg cgtgatacac cctgacggtt atatccagct
     9481 caaggaccgg tcgaaggaca tcatcatctc gggtggcgag aatatttcct cgatcgaggt
     9541 ggaggacgtt ctctacaagc accccgcggt cgccgccgcc gccgttgtcg ccaagcccga
     9601 tacgaaatgg ggcgagaccc cttgcgcctt cgtggagacc aaggcgggcg caaaagtcac
     9661 cgaagatgag atcatcgcct ggtgcaagga gaagctcgcc cgctacaaat gcccccgcca
     9721 cgtcgtgttc atcgagctgc ccaagacctc gaccggcaag atccagaaat tcaagctgcg
     9781 cgacatggcg aaagccgtgt aggcctcgcg gctatcaacg aacttggcac ttcaagcttg
     9841 ccgatccgac gttcgatctc cgtcaagcgg catcggccgg gcgcacgcgc ttcgcgattt
     9901 catcgcccgc tccgattcga tcagggcgat atcatgctgg ctttgcagat cgagccagaa
     9961 ttgcgctcgg ttgccgaaat agcggccaag gcgcaacgcc gtgtcggcgg tgatcgagcg
    10021 ccgtccgttg agaatgtcgg tgatgcggcc cgacggcacg ccgagatcga gcgctagccg
    10081 gttcgcgctg aggccgcggg cttgcaactc gcgcttgaga aggcggccgg gatgcacagc
    10141 gagtaccaat gttcctcacc cctttcgcgg gggcgtacca taacgccaca aaccggttac
    10201 tacgtgatcc cataaaccgc aatctgtgca tggggatgaa agcgggactt gcgtggcaca
    10261 gttgcttggc atcaatctga aggggctttg ggggaaccgg ccatagggct cgaggaacaa
    10321 ttcaaggaga ttgcggggtt tgtcgccctc gctatcgagg cggcggcggt tctcgtcgtg
    10381 agttacggcg cgctccaggc gctggccgga ttggtgggat ccgccgctca gtcgcaccgc
    10441 agacgagatg atcggccggg agatctggct caaattcgcg acctggatct tgctcgcact
    10501 cgaatttgcc cttgccgccg atctcgtgcg cacggcggtc gcgccgacgt gggacgacat
    10561 ctcgaagctc gcggtgatcg cgacaatccg caccatgctc aactacttcc tggcgaagga
    10621 catcgccgag ttcgaccggg cgcgccaaac cggcggaaat ccgccgtcgg gccgggttgc
    10681 ctcctaatcg attggaccgc cttcagataa tatgtttgag tgagtcgcag ccctggaaaa
    10741 gggctagaaa cggccttggg gggcggtttc cggcctgtct tgagagcctc atgagccgca
    10801 gcgccacggc acggcgcgcg gggggtggcc cgaaaagaat ttgtcgtgcg aggcgtagaa
    10861 gacacctcgc ttgggattga gggaatgatc agcaagacca gcacgagcgg cccacgcttt
    10921 cagctcatcc tgatcaagcc gtcgcattac gacaatgaag gctatgtcgt gcagtggctg
    10981 cgttcgacca tgccatcgaa ctcgcttgcc gccgtcttta gccttgcgcg cggcgcggcc
    11041 gagcggcagc tgctcggccc cgaccttccg atcgacgtca tggccatgga cgagaccaac
    11101 acccgcgtgc gcccgcgcga gatcgcgcgg cgcatcgctg agaatggcgg aaagggtttg
    11161 gtcggcatcg tcggcgcgca gtcgaacgag ttcccccgcg ccgtcgacat tgcgcggccc
    11221 ttgcgcgcgg ccggcatcca agtcctgatc ggcggcttcc acgtttcggg ttgccttgcc
    11281 atgctgaaag agctgcccga ggacatcaag gaagcgcaga ggctcggcgt ctcgatctat
    11341 gccggcgagg ccgaagacgg attcgacgaa gcgatcctcg acgccgcacg cggccaattg
    11401 cgcccactct acgatcacat gaagcacctg cccgatatcg gcgaagtcgc ctctccgcct
    11461 ttcctgcccg tcgatttcgt ccgccgcacc atcggcaatg tcacgagctt cgacgcgggg
    11521 cgcggctgcc ccttccagtg ctccttctgc accatcatca acgtgcaggg gcggaagtcg
    11581 cgctatcgct cccccgacag cgtcgagcag atcatccgca tgaattacga gcagggggtg
    11641 aaccgcttct tcatcaccga cgacaatttc gcgcgcaaca aggactggga ggcgatctac
    11701 gaccgcatca tcaagctgcg cgaagaggac gggcttgaca tccgcttctg catccaggtc
    11761 gacacgctct gccaccgcat cccgaacttc atcgacaagt cgcgccgcgc cggggtcacc
    11821 cgcgtgttca ttgggctcga gaacatcaac cccgccaatc tgatcgcggc caagaagcgg
    11881 cagaacaaga tcaccgagta ccgcaagatg ctgctcgaat ggaaacgcgt cggcatcatc
    11941 acctatgcgg gctacatcct cggcttcgag aacgacacgc cggagtcgat ccggcacgat
    12001 ctcgagatca tcaagaaaga gctgccgctc gacatgctgg aattttttat cctcacgccg
    12061 ctgccgggct ccgaggacca caaggtgctg tgggaaaagg gcgtgtggat ggaccccgac
    12121 atgaacaaat acgacgtcga gcacgtcgtc accgcgcatc ccaagatgag cgcagcggag
    12181 tggaaggagg cctatcgcac cgcctggggc cactactata ccgacgacca tctggagacg
    12241 atcgtccgac gcgcctatgc gagcggaatc aatatccgct cgctgatgcc ggtcctattc
    12301 tggttttcga gcgcggtgcc ggtcgaaaat ctgcatccgc tgcaatgggg catcttccgc
    12361 gtcaaatacc gccacgaccg gcgctcaggc ctgccgctcg aatcggcgct tgtgttctac
    12421 agccgctatg ccgccgacat cgcccgcaag gtcgttgtcg tggcgcggcg ctggcggcat
    12481 ctgaagcgga tcgtgcgcaa gatcgaggcc gatccgcgcg ccaaacttta tatggacgaa
    12541 gcgctgaccc cggtgactga ggaagactcc gagcatatgg agctctacac gcagaacgag
    12601 gctgcccgca gcgcggtcga acgcgagctt cgcgtagcga gaaagagcgc caacggccat
    12661 ggccgcaagg ccccggtcac atccgatcaa ccgggcaacg ggcgtcagcc gggcggccgc
    12721 gatgccggcc cttcatccgc cgcgccttcc ctcagcgatc agagcgagcc gatttcgccc
    12781 atcgtctgag cgctcgcgcg atggctgtgt cagtgcaagc ggctgtgatg atggcttcgc
    12841 gagcgccgat gcggcccgcg tgcgttcaaa gaccgccaag ggcccgattg atcatgtcgc
    12901 cgaatgcgtc gccgttccgc cgccaccagc cccggcgccc gcgcggctcc tcctcataca
    12961 tcgcctcggc atcgtaggga gtccccggcg ggggagcggg agcgagcaag tgcgcctgca
    13021 tccaaccggt tgcggcgctc tgcggatcgg tgacttcgac ccagccttcg taacgcgaca
    13081 ccaccctgag cacgcggccg taagggaagg cgaacagcat gggcgcatcc tcggccgccg
    13141 taccatgcat gcccgcgccc gacacgagca cctgcaccca ttcctgcggc tcctcctcgg
    13201 gcccgccagg ccaggcgttg ggatcgacat cctccctgag cgccgccgcg ggatccgacg
    13261 gatcgtcata cgcgccgggc ggcggtggca taggatcggt cccctcggcc tccatgccgg
    13321 gaggtgcatt gggcggtcct tggtgctgcc agggaagagc gttcgggtcg ctcgcctcgc
    13381 cgtcgtcggg cggcaacgcc gccatctccg tttcgctctg ctccgaggcg gggacgttcg
    13441 ctccggctgc tggcgcgggc gcagcttcag ggccctgagc gggggctgtc gccgccggaa
    13501 cggatgcctt gggagtggcc gcttgctctt gcgcgacagc cggtttggtc tcctcagggg
    13561 tccgcgcgat cttctcctca taggcgagcg tccgcgatga cggctcgctc cccacccgct
    13621 tggtcgcggg cgcggggctc accaccatgt agagggccac acacacgacc gcgctgaggg
    13681 ccaacagcga aacaatgagc ttgttaatgg cagatctccc cgtcccttgc cgtcattagc
    13741 cccggaaaac gcggcaacag cgtgtcatcg ccgtccatta tcgatcggtt aagcctaatt
    13801 ttcggtgaac cggcgaggca ggtccagctt caaaaaaaaa aggcgagccg
//
LOCUS       DASUQP010000270         6261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1633605, whole genome shotgun sequence.
ACCESSION   DASUQP010000270 DASUQP010000000
VERSION     DASUQP010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633605"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFY74_12190"
     CDS             complement(<1..613)
                     /locus_tag="VFY74_12190"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611611.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX5932486.1"
                     /translation="MSPAKASVTPLAPRDGAHPIEPDFPSFARRYDEGVPQVAWTRLV
                     ADLETPVSAMLKLAHERPNSFLLESVEGGATRGRYSIIGCEPDLIWRASGATAEINRA
                     PQSDPDAFAPSPVPTLASLRALLAESAIALPPELPPMAAGIFGYMGYDTVRLIERLPG
                     ETPDRLGLPHSILVRPTLIVIFDSVTDEMTVVTPVRPAPGVGAK"
     gene            complement(610..2508)
                     /locus_tag="VFY74_12195"
     CDS             complement(610..2508)
                     /locus_tag="VFY74_12195"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5932487.1"
                     /translation="MALESLRKGAGGIIGIGFVLVLIASFIIWFPAGWFQGYGAQDVI
                     VVGDVKIGPQEYMRAQQDMLRVMSTQAGRSLSLQEARAAGLNRLVVARLVGGAAIDMH
                     ARALKLGISDAALLEEIMKDPAFKDGSGNFNPGALQQAMYALNMNEQSFLASMREQNL
                     RQQILTTVGEVVNTPTLLVDALNSYNGESRTLRYVLVPESAAGTIPEPSDEDLRRYYE
                     NHRSKFTQPEFRKIGVLAVTPETMKEQLQITEADIKTAFETKKDKLGTPEKRLIQQIP
                     FPDLAAANAAYQKIQSGTDFLAVAKEQGVSEADIDLGTLARAELADPAIAEAAFALEP
                     NKVSEPVTGKLGSIVLLRVTAVQPGKTPTLEESKADLEKTLLKERASSVILDLHDKIE
                     DLLASGATLSEIADKLKLSYQVVDQVDREGRKPDGSAVTLPRQKELLNAAFATDAGVE
                     NDPIDAQDDGVIWYEVMGVVPEQLKPFDQVKDQVAKDWRAHEARTRTAKYAQDLVTSL
                     NSGKTLEDVAKDLNVEVLTSDPLKRESITVNVLPASVAQAFALPEKGYGSGPSGVDEG
                     RIVFQVDKVTPPAPISATELERIKKQITLLMSQDAIEEYSGALEKRYGVTINQQALAK
                     LIGSDDEP"
     gene            2692..3465
                     /gene="tpiA"
                     /locus_tag="VFY74_12200"
     CDS             2692..3465
                     /gene="tpiA"
                     /locus_tag="VFY74_12200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5932488.1"
                     /translation="MAAKTPLVAGNWKMNGLGAALSELRALKERLAQGLAPDAEIMVC
                     LPATLLSRARDVLAGSDIKLGAQDCHPLPAGPHTGNISAEMLADAGASAVIVGHSERR
                     RGHGETDALVNAKTRAAFRAGLTAILCIGETEQERLNGATLEVVGRQLEGSLPRDARA
                     ANCVIAYEPVWAIGSGLTPTPADVAAVHRFIRGELVRLLGSAEGRELRILYGGSVKPA
                     NAGELLSVPDVDGALVGGASLKASDFFAILSAYCGRHAG"
     gene            3566..4072
                     /locus_tag="VFY74_12205"
     CDS             3566..4072
                     /locus_tag="VFY74_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932489.1"
                     /translation="MLLLRFIAVALLAGTFAVLPGARSRAEPLDREACLELERQRSKL
                     LTREMKAALERGPDWVKDHLDTGEIEKVREFLSVEEMIQFRCRGGGRVKQTATPGSGE
                     AVPLPDRKPDPPQGTDDAGTVAAGSADMPLPDRKPGSATPAMGDVEPSQALVDSDKTA
                     PSKTEAAR"
     gene            4069..4422
                     /gene="secG"
                     /locus_tag="VFY74_12210"
     CDS             4069..4422
                     /gene="secG"
                     /locus_tag="VFY74_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX5932490.1"
                     /translation="MSTVLLLIHVMIAVALVGVVLLQRSEGGALGIGGGGAGGFMTGR
                     SAGNALTKTTAVLAACFFATSLALSILAMNQKPAPTILPGGGGGLAPLQVPAAPAPAT
                     PQAPSAPSAPQAPQP"
     gene            4528..6153
                     /locus_tag="VFY74_12215"
     CDS             4528..6153
                     /locus_tag="VFY74_12215"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5932491.1"
                     /translation="MARYVFITGGVVSSLGKGLASAALGALLQARGFSVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRPASQADNITTGRIYQEIIVKE
                     RRGDYLGATVQVIPHVTDAIKSFITSGNEGVDFVLCEIGGTVGDIEGLPFFEAIRQLG
                     NELPRGSTAFIHLTLVPFIPSAGELKTKPTQHSVKELRSIGIQPDVLLCRTDRPLPRD
                     ERRKIALFCNVRPEAVIQALDVPSIYDVPVAYHAEGLDREVLAAFGITNAPEPDLERW
                     REIAQTIANPDGEVTVAVVGKYTGLKDAYKSLNEALVHGGIANKVKVHLQWIESQTFE
                     SEDASAYLEDVHGILVPGGFGERGAEGKIRAARYARTRRVPYFGICFGMQMAVIDAAR
                     EAGIEGASSTEFGPTEEPVIGLMTEWMKGEELQKRQSNGNLGGTMRLGAYDAVLAKGS
                     KVAEIYGATRISERHRHRYEVNMRYRNKLEAAGLRFSGLSPDGLLPEIVEIPDHPWFL
                     GVQFHPELKSRPFSPHPLFASFIGAAVEQSRLV"
BASE COUNT         1017 a         2066 c         2068 g         1110 t
ORIGIN      
        1 ccttggcgcc caccccgggc gccggtctca ccggcgtcac caccgtcatc tcgtctgtca
       61 cgctgtcgaa gatcacgatc aacgtcggcc gcacgaggat gctgtggggg aggccgagcc
      121 tgtcgggggt ctcgcccggc aggcgctcga tcagccgaac cgtgtcgtag cccatataac
      181 cgaagatgcc ggcagccatg ggcggcagtt cgggcggcag cgcaatcgcg gattcggcaa
      241 ggagggcgcg gagcgacgca agcgtcggca ccggcgaagg cgcgaacgcg tcgggatcgg
      301 actgaggcgc ccgattgatt tcggccgtgg ctccactcgc ccgccagatg agatcgggct
      361 cgcagccgat gatcgaataa cggccgcgcg tggcgccacc ctcgaccgat tccaggagga
      421 agctgttcgg ccgttcatgc gccagtttga gcatggccga gacgggggtc tctaggtcgg
      481 cgacgaggcg cgtccacgcc acctgcggca cgccttcgtc gtagcggcgc gcgaagcttg
      541 gaaagtccgg ctcgatgggg tgagccccgt ctcgcggcgc gagcggcgtg acggaggctt
      601 tcgccgggct catggctcgt cgtcgctgcc gatgagcttg gcgagggctt gttgattgat
      661 cgtcacgccg tagcgtttct ccaacgcgcc ggaatactct tcgatggcgt cctggctcat
      721 gagcaacgtg atctgcttct tgatgcgttc gagctcggtc gcactgatcg gcgcgggcgg
      781 cgtgactttg tccacctgaa agacgatgcg gccctcatcg acacccgacg gccccgatcc
      841 gtaacctttc tcaggcagag cgaaagcctg cgccaccgaa gcgggcagca cgtttacggt
      901 gatgctctcg cgcttcagcg ggtcgctggt caggacctcg acattgaggt ccttggccac
      961 gtcctcgagc gttttcccgc tgttcaggct ggtgacgaga tcttgcgcat atttcgccgt
     1021 cctggttcgc gcctcgtgcg cgcgccaatc cttggcgacc tggtccttga cctgatcgaa
     1081 cggtttgagc tgctcgggca cgacgcccat tacctcgtac cagatcaccc cgtcgtcctg
     1141 ggcgtcgatc ggatcgttct cgaccccggc atcggtagcg aacgcggcgt tcagaagctc
     1201 cttctgccgg ggcagcgtaa cggccgagcc gtccggtttg cggccctcgc ggtcgacctg
     1261 gtcgacgacc tggtagctga gcttgagctt gtcggcgatt tcggagagcg tcgctcccga
     1321 tgccaggaga tcctcgatct tgtcatgcag gtcgaggatc acgctcgaag cgcgctcctt
     1381 gagcagcgtc ttctcgaggt cggccttgga ctcctccaga gtcggggtct tgccgggctg
     1441 gaccgccgtc acgcgcagca gcacgatgct tccgagcttg cccgtgaccg gctcgctcac
     1501 cttgttcggt tcgagggcaa aggcggcctc ggcgatggcc ggatcggcca gctcggcgcg
     1561 ggcaagcgtg ccgaggtcga tatcggcctc gctcacaccc tgctccttgg caaccgccag
     1621 aaagtcggtc cccgactgaa tcttctgata ggcggcgttc gccgcggcga ggtcggggaa
     1681 ggggatctgc tggatgagcc gcttctcggg cgtgccgagc ttatccttct tggtctcgaa
     1741 ggcggtcttg atgtcggcct ccgtaatctg aagctgctcc ttcatggttt ccggcgtgac
     1801 ggcaaggacg ccaatcttgc ggaactcggg ttgcgtgaac ttgctccggt gattctcgta
     1861 atagcgcctg agatcctcat cgctcggttc gggaatggtc ccggcggcgc tttccggtac
     1921 cagcacgtag cgcagcgtgc gcgactcgcc gttatagctg ttgagcgcgt cgaccagcag
     1981 cgtcggcgtg ttgacgacct cgcccacggt tgtgaggatc tgctggcgca aattctgttc
     2041 gcgcatcgag gcgagaaagc tctgctcgtt catgttgagc gcatacatcg cctgctggag
     2101 ggctcccggg ttgaaattgc ccgacccatc cttgaaggcg ggatccttca tgatctcctc
     2161 gaggagcgcc gcatcgctga tgccgagctt gagcgcacga gcatgcatgt cgatcgcggc
     2221 acccccgacc aaccgggcca ccaccagccg gttgagtccg gccgcgcgcg cctcttgcag
     2281 actgaggctc cgccccgcct gcgtgctcat cacccggagc atgtcctgtt gcgcccgcat
     2341 gtactcttgc gggccgatct tcacgtcgcc gacgacgatg acgtcctggg cgccgtaacc
     2401 ctgaaaccac ccggccggaa accaaatgat gaagctcgca atcaggacga gaacgaaacc
     2461 gatcccgatg atgcctcctg cacctttgcg caaactttca agtgccacgc tctggaacct
     2521 tccattcgtc atttgccaaa gccgcgctgg cgtccgatgc taggacttcc tcgcgcggcg
     2581 gcggcatcat aggtagcaag ggcaagcccg gcaaggcggg gtactggcct cgccaaggtc
     2641 cggcgcaggg tcgaatagcg cttccgcggg ggcgcggtcg gagtggggaa attggcagcg
     2701 aagacgccgc tcgtcgcggg caattggaag atgaatgggc tcggggccgc cttgagcgag
     2761 cttcgagcgc tcaaagagcg cctggctcag ggcctggcgc ccgacgccga aatcatggtt
     2821 tgcctgccgg cgacgctgct ttcgcgggcg cgtgacgtgc tcgccgggag cgacataaag
     2881 ctcggcgccc aggactgcca tccgctccct gccggacccc acaccggcaa tatctccgcc
     2941 gagatgctgg ccgatgccgg tgcaagtgcc gtgatcgtgg gccattcgga acggcggaga
     3001 ggccatggcg agaccgacgc tctggtgaac gccaagacgc gcgcggcctt ccgcgccggt
     3061 ctgaccgcca tcctctgcat cggcgagacc gagcaggagc ggctcaacgg cgcaacgctg
     3121 gaggtcgtcg gtcgccaact cgagggctcc ctgccgcggg acgcgcgcgc ggcgaactgc
     3181 gtgatcgcct acgagccggt ttgggccatc ggctccggcc tcacgcccac gcccgccgat
     3241 gtcgccgcgg tgcatcgttt cattcgcggc gagcttgtcc gcttgctggg cagcgcggag
     3301 ggccgcgagc tccgcatcct ttacggcggc tcggtcaagc cggcgaatgc cggagaactc
     3361 ctctcggtgc ccgacgtgga cggggcgctc gtcggcggcg cgagccttaa agcgagcgat
     3421 ttcttcgcca tactgtccgc ctattgcggc cggcacgctg gctgatctct cacgcgcccg
     3481 cgcgagcggg gttggcaaag gcgacgattt tcgccattct gagacagcga cttgcggcgg
     3541 ggggacggtc cgccgggaca ttgcgatgct cttgctgcgc ttcattgccg tagcgcttct
     3601 cgccgggacg tttgccgtgc ttcccggcgc gcgttctcgt gccgagccgc tggacaggga
     3661 ggcctgcctg gagctggaac gtcagcgttc gaagttgctc acacgcgaga tgaaggcggc
     3721 tctcgagcgc ggtcccgatt gggtgaagga ccatctggac accggagaga tcgagaaggt
     3781 gcgggaattt ctctccgtcg aggagatgat ccagttccgc tgccgcgggg gcggccgggt
     3841 caaacagacc gcaacgccgg gaagcggcga ggcggtaccc ttgccggatc gcaaacctga
     3901 cccgccgcag ggcacggacg acgcgggaac ggtcgccgcc ggaagcgccg acatgccgct
     3961 cccggaccgc aagcccggtt ccgcgacccc cgccatggga gacgtcgagc ctagccaagc
     4021 cctggtcgat tcggataaga cagcgccttc gaaaacggaa gcggcacgat gagcactgtt
     4081 cttctcctga tccatgtcat gatcgccgtg gcgctcgtgg gcgtcgttct gttgcagcgt
     4141 tccgaagggg gagcgctcgg gatcggcggg ggcggtgcgg gcgggttcat gaccgggcgg
     4201 agcgccggca acgcactcac caagaccacc gcggttctcg ccgcctgctt cttcgccacg
     4261 agcctcgcgc tgtcgattct ggcaatgaat cagaagccgg cacccaccat tctgccaggc
     4321 ggaggaggag ggctcgcgcc gctccaggtt ccggcagcgc ccgcgcccgc gaccccgcaa
     4381 gcgccctccg caccatccgc gccccaggcc ccgcagccct aacaacgttc ggcgtccgaa
     4441 cttccgggga taacgcgcgc gagccgccgg ccgaaccgct gtttggggct tacgactcag
     4501 ccgcgtttcg gtgtatggtc gaggcccatg gcgcggtacg tcttcattac cggcggcgtg
     4561 gtttcctcac tcggcaaggg gctggcctcc gcggcgctcg gtgcgctgtt gcaggcccgc
     4621 gggtttagcg tgcgcttgcg caagctcgac ccctatctca acgtcgatcc ggggaccatg
     4681 agcccctatc agcacggcga ggtgttcgtc accgacgacg gtgccgaaac cgatctcgac
     4741 ctcggtcatt acgagcgctt caccggacgg cctgcctccc aggccgacaa catcaccacg
     4801 gggcggatct atcaggagat catcgtcaag gagcggcgcg gggactatct cggcgcgacc
     4861 gtgcaggtca tcccccacgt caccgacgcc atcaagtcct tcatcacttc cggcaatgag
     4921 ggcgtcgact tcgtgctgtg cgagatcgga ggcacggtcg gcgacatcga aggcttgccg
     4981 ttcttcgagg cgatccgcca actcggcaac gagctgccgc gcgggtcgac ggccttcatt
     5041 catctcaccc tggtcccgtt cattccgagc gcgggcgaac tcaagaccaa gccgacccag
     5101 cattccgtga aggaactgcg ctcgatcggc atccagcccg acgtactgtt gtgccgcacc
     5161 gatcggccgc tgccgcggga cgagcggcgc aagatcgcgc tcttttgcaa cgtccgtccc
     5221 gaagcggtga tccaggcgct cgacgtcccg agcatctacg acgtgcctgt cgcctatcac
     5281 gccgagggac ttgaccgcga agtgctggcg gcgttcggca tcacaaatgc gcccgagccc
     5341 gacctcgagc gctggcgcga gatcgctcaa accatcgcca atcccgacgg cgaagtgacg
     5401 gtcgccgtgg tcggcaaata taccggactg aaggacgcct ataagtcgtt gaacgaggcg
     5461 ctcgtccatg gcggcatcgc caacaaggtg aaggtgcacc tgcaatggat cgagtcgcag
     5521 accttcgaga gcgaggacgc ctcggcatat ctggaggacg tgcacggcat cctcgtgccg
     5581 ggcggatttg gcgagcgcgg cgccgaaggc aagatccggg cggcgcgcta cgcgcgcacc
     5641 cgccgcgtac cttacttcgg catctgtttc ggcatgcaga tggcggtgat cgatgccgcc
     5701 cgggaggcgg gcatcgaagg ggcgagctcg acggagttcg gcccgaccga ggagcccgtc
     5761 atcggtttga tgaccgaatg gatgaagggc gaggagctgc agaagcggca gtcgaacggc
     5821 aatctcggcg gcaccatgcg gctcggcgcc tatgacgcgg tgctcgccaa gggcagcaag
     5881 gtcgccgaga tctacggcgc gacccgcatc tcggagcgcc accgtcatcg ctacgaagtg
     5941 aacatgcgct accgcaacaa gctggaagcg gcgggcttgc gcttctcagg cctctcgccc
     6001 gacgggctct tgccggagat cgtcgagatc cccgaccacc cgtggttcct cggcgtgcag
     6061 ttccacccgg agctcaagtc gcggcctttc agcccgcacc cgctcttcgc ctcgttcatc
     6121 ggcgcggccg tggagcagag ccggctggtc tagccggctc gctttcgcgg tcagcggcgt
     6181 ttgcgcggcg ccgaggccgc cggcggaacg tcggacttgg cactgccccg cacgggaatt
     6241 tcttctcccg ccgcaaggat g
//
LOCUS       DASUQP010000271        24453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684 k141_135760,
            whole genome shotgun sequence.
ACCESSION   DASUQP010000271 DASUQP010000000
VERSION     DASUQP010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 24453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24453
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135760"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..2537)
                     /locus_tag="VFY74_12220"
     CDS             complement(213..2537)
                     /locus_tag="VFY74_12220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5932492.1"
                     /translation="MLRIRKVADATTAVNRSAIEAAQRIMRQQFKAIPEEDIAKLPDQ
                     FANPLKHKFVSRLFVAENERDQTLGVALLLHAPDIGFSYLEIISTAPGRMGAGFGAAL
                     YERVREEARALGTQLYFECLPDDPALSPNTEVRAANAARLKFYERYGARPIVNTAYET
                     PVRAGTTDPPYLVLDPLGAGDLPSRDKVRKVVRAILERKYNCPPDYVQMVVGSIKDDP
                     VRLREPRYVKTRRALRGEMRAASAPRIALVLNDEHTLHHVLDRDYFEAPVRIRTIMAE
                     LDPSGLVEKVPAKRYSDRHIRAVHDSRLVDYIRKACLIAGPKKSIYPYVFPLRNPARA
                     PKDETVLAGYYCIDTFTPLNQNAYLAARSAVDCALTAAEKLLQGAPLAYALVRPPGHH
                     AETRSFGGFCYFNNAAIAANLLARYGKVAILDIDYHHGNGQQEIFYNRADVLTVSIHA
                     HPSFAYPYFTGFRDESGIGPGAGFNLNIPLPEHITPEQHRNAVAEGLRRVKRFAPAYL
                     LVSLGFDTARGDPTGTWSNRAKDFEQLGRMIGEQGFPTLVVQEGGYRVRTLGSNARNF
                     FAGLVAGHSAARLAAPALAKVSGPSRNTARDGLDWRSAVMADDVGRVRSLVASTGFFN
                     AAEVETASDLVTERLTKGIRSGYSFVLAERGANLVGFACYGPLHGTQGSFELFWIAIA
                     PEEQKKGLGAQVYARAEAAMVKASAKHIYADTSSSDRYADTRGFYERMGFREGARLPD
                     FYAPGDGKIVFVKDLAADTQPPRQTPSGEPFSSR"
     gene            complement(2557..3543)
                     /locus_tag="VFY74_12225"
     CDS             complement(2557..3543)
                     /locus_tag="VFY74_12225"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5932493.1"
                     /translation="MHGSAGFVPVLHAATESRPDEIDTIIAAEAVAEAIARLGYATEI
                     VGLGPDLSGLEALASRRPLLVFNLVEAVGGDCRLAPMVPARLDALALPYTGARTSACL
                     DTLSKIGTKLRLAHAGLPTPEWSQDGTGLDPEARVIVKPVFEHGSLGLEPASVMRGAE
                     AARAIVERTLRWRTEHFAEAYIDGREFAVAMIERPQRVEVLPIQETLFQGFGRGEPLI
                     ADYDAKWTPGSQPYVGTPRRFGLETDDPELAADLTRLGLACWSLFALDGYARVDFRVD
                     RTGAPFILEVNVNPCLSADAGFAASALEAGIDYDALIARVIEAPLGRLQATA"
     gene            complement(3543..4556)
                     /locus_tag="VFY74_12230"
     CDS             complement(3543..4556)
                     /locus_tag="VFY74_12230"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5932494.1"
                     /translation="MRVALVYDLRNDYRALGFSEEEVAEFDSVETIDALAGALEALGC
                     EPIRVGRGQALAARLVAGERYDIVFSIAEGVRGRSREAQVPALCELFDQPYLFSDPLT
                     AAAALDKAVTKRLVRDAGVPTPSFAVAERSANELVDWVDFPAFVKPLAEGTGKGCEAA
                     SLVDSKGALETAVAHVIARYRQPALVEQYLPGREFTVGIVGNGDKARVLGVCEILLKP
                     NAEANVYSLHNKELCEELVTYAPATDAEARLAGTRALQAYRALQCRDAARIDFRSDAK
                     GDPHFLEANPLAGLNPSHSDLPILAAQNGIDFTSLMGLILDAGLARYGLARSKQQPRR
                     KRA"
     gene            complement(4562..5893)
                     /locus_tag="VFY74_12235"
     CDS             complement(4562..5893)
                     /locus_tag="VFY74_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX5932495.1"
                     /translation="MKLLQVEAEEPPSRPKATSRLLIKPHSPINALSPAPALPAKAPR
                     LDAVVVRMERFPIGPLSRAFRKRYYPEVDTATWNDWRWQTRARIRTLAELERIFVLSE
                     DEREAVIRHQGSLPLGITPYYASLMAREDAAEPVRRTHIPVGDEYLKTPGEADDPLGE
                     DGHTTVPGLVHRYPDRVLFLATGFCSTYCRYCTRSRMVGEAGGEYTFSRPQWEEALNY
                     IASHPEIRDVLISGGDPLTLSDERLDYLLGRLRAIKHVEFVRLGTKVPVVLPMRVTRA
                     LIRVLKKHHPLWMSLHFTHPSELTPEVTEATARLADAGIPLGSQTVLLKGINDEVETL
                     KSLFHGLLMRRVRPYYLYQCDPISGSAHFRTSVDKGLELIRGLRGHTTGYAVPTYVVD
                     APGGGGKIPLLPDYVAGREGDDLLLRNFEGNIYRYTDPEGTVGIGRTPGAG"
     gene            complement(6072..7370)
                     /locus_tag="VFY74_12240"
     CDS             complement(6072..7370)
                     /locus_tag="VFY74_12240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX5932496.1"
                     /translation="MTPAARASAAMEILADIEARKRPAAEALKDWGTSHRVAGSGDRA
                     AIGNLVFDALRTRASAAHAMGEGSPRALVLRSLVTRWQMTPEQVAQLADGSRYAPEPL
                     SEAELAGLKLEIPATAPAHIRGDYPEWLGPEFERAFGARAAEQGAGLARRAPVDLRVN
                     TLKADRDKVLKALRRFEVGPTPHSPFGVRAAQASGPGRSPHLEAEPGHGKGWYEVQDE
                     GSQLATLLSGAGPRQQVIDLCAGAGGKTLALSALMQNTGQLHAYDADALRLRPIFERL
                     KRAGARNVQVLPPGEREALGKLAGKMDLVLIDAPCSGSGVWRRRPDAKWRLSLSMLEA
                     RRGEQRVVLEEGAALVKPGGRLAYVTCSVLPSENRDQIDGLLARRPEFELIPWPELWE
                     RSLPGQAPAPSADGSGETLLLTPRDHGTDGFFVAVLQRAG"
     gene            complement(7370..7798)
                     /locus_tag="VFY74_12245"
     CDS             complement(7370..7798)
                     /locus_tag="VFY74_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX5932497.1"
                     /translation="MSIHAILMPMFVQVGLTFTLLFWMAALRVRAIQAGEVDPEKIRL
                     REPHWPARVLQISNAYHNQLELPVLFYVVVLLAFASQTLDVTLLVLSWLFVLSRLVHA
                     YIHVTSNRLDRRTGVFGISAIALLLMWVIVIARIILFTGS"
     gene            8105..8935
                     /locus_tag="VFY74_12250"
     CDS             8105..8935
                     /locus_tag="VFY74_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5932498.1"
                     /translation="MRRRALYAVSCAAIAGALAFAAFPRPGHAADNPCFTGEGSIKEI
                     LDGCGAYIGSGATDKDQLVRAHAVRAMAFSAIRDLDSAIAELDEAIKVDAIRANSYFM
                     RAAAHEAKKNHETALKDLDEAIRLDAKQADYYLLRGIIYRDKGDLDRALVDFDEKVKL
                     EPNAALGYSKRGDLYRQRKEYDLAIADFDQVIKIDDKDAKGYIDRGWVFVLKNDLDKA
                     GADFDNALKLHENEPSALVGRGLVKSRKGQPTDGSADLALAQRLEPGIFDEIKKLGVE
                     "
     gene            complement(9184..10701)
                     /gene="guaB"
                     /locus_tag="VFY74_12255"
     CDS             complement(9184..10701)
                     /gene="guaB"
                     /locus_tag="VFY74_12255"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5932499.1"
                     /translation="MPEGQTPEKRKQFPDSGIALTFDDVLIRPSASNVLPNEADVSSR
                     ITRSIALHIPVVSSAMDTVTEAKLAIAMAQAGGIGVIHRNLDPAAQAAQVAQVKKFES
                     GMVVNPVTIHPGATLADALKLMVDHQISGIPVVEPAGNGGREGRLAGILTNRDVRFAT
                     NPNQRVAELMTKDKLVTVRENVGLEEAKRLLHQHRIEKLLVVDDAYRCIGLITVKDIE
                     KARAHPNACKDEQGRLRVAAATSVGDSGFDRAERLIDAGADVIVVDTAHGHSALVLDQ
                     VRRIKRQSNAVQVVGGNVATADGTKALIDAGADAVKVGIGPGSICTTRVVAGVGVPQF
                     TALLEAVEVAKKSDIPVISDGGIRYSGDLAKALAAGADCVMLGSLLAGTDESPGEVFL
                     YQGRSYKAYRGMGSIGAMARGSADRYFQQEIRDALKLVPEGIEGQVPYKGPVSSVLHQ
                     LIGGLRASMGYTGARTLAEFQARAEFIRISAASFRESHVHDVTITRESPNYPREG"
     gene            complement(10820..11731)
                     /locus_tag="VFY74_12260"
     CDS             complement(10820..11731)
                     /locus_tag="VFY74_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5932500.1"
                     /translation="MDRLAALRLFVTVAEAGSLSAAGRKLGMPLSTVSRHLKALEEEL
                     DTRLITRTTRRLTLTESGRAYAATCRQVLDELDDAGRRLTGEHAEPRGELALTAPVVF
                     GRLHVLPIVAAFLNAFPRVAARMLLVDRVVDLVEEGIDIGVRIGNLPDSTLRATRVGA
                     IRLIACASPTYLAQHGTPASPSELAGHQCISFNTFTHAERWTFGERGKRELRVTVSPR
                     LVVNSADAAIDAARAGLGIVRVLSHQGEASIANGALQRILEAFEPEEIPVNLFHREDR
                     LPQAKVESFIAFAAPRLRQVLRSGRKG"
     gene            11853..12455
                     /locus_tag="VFY74_12265"
     CDS             11853..12455
                     /locus_tag="VFY74_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5932501.1"
                     /translation="MAQLKLHDLELSGNCYKVRLFCSLLGLKLDLVPVDFLAGAHKKS
                     PLIEKNPFGEIPILEEGDVVLRDSQAILVYLARKYGGEQWLPTDPVSMGRVVAWLMVA
                     ENEVARGPNDARLHDKFGFKLDASAALEKAKRIFGLMESHLAKNDWLALDRPTIADIA
                     CMPYMALSHEGGLSLEPYPAIRAWIRRIKALPGFITMPAL"
     gene            12463..13086
                     /locus_tag="VFY74_12270"
     CDS             12463..13086
                     /locus_tag="VFY74_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5932502.1"
                     /translation="MSSSTDVAFTLSVKRLQDRRGSRGAYARMEEKGGFATKIDDGLA
                     AFIANKRSFYFATANRNGQPYIQHRGGPKGFLRVLDPTTLAFADFGGNRQYITTGNLA
                     DNPRAMLFLMDYTQRRRVKIWGTARAVEDDAELIAKLFPDAYKARAEQAIVFTVAAWD
                     ANCPQHIPQMLFAEDVAEAFAERDRRISALEAEIALLRAAPAGMENL"
     gene            complement(13022..13756)
                     /locus_tag="VFY74_12275"
     CDS             complement(13022..13756)
                     /locus_tag="VFY74_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX5932503.1"
                     /translation="MQIDLARRLSAEALGTFILVMAVVGSGIMAEMLAGGNVALALLC
                     NTVATAAILVVIITIFVSVSGAQFNPAVTLVMLLRGEIESRTALLYVAAQVVAGCLGA
                     VAAHLMFDLAPVQISGNARTGFGQWLGEFIATFGLVATILGCLRFRPGALPAAVGLYI
                     GSAYWFTSSTSFANPAVTIARALSDTFSGIAPADASGFIVAELLGAVAASYFFGWLFA
                     EPAAEAKDSPSPREQRAGGRSRLQAR"
     gene            complement(13825..14589)
                     /locus_tag="VFY74_12280"
     CDS             complement(13825..14589)
                     /locus_tag="VFY74_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX5932504.1"
                     /translation="MVRPGKGQGGERQGGHRKLAVRVRTAKGRKLASTRWLQRQLNDP
                     YVEEARRRGYRSRAAFKLIEIDDKHHLLRPGMTVVDLGAVPGGWSQIAAQRVGLMAGK
                     GRVVAVDLAAMEPIAGVVQLELDLTDWDASRRIGEALGGERADVVLSDMHAPATGHKA
                     TDHLRNMGLVEAALDLAESILAPGGTFLSKVLQGGASRELVARLNRSFAKVRHVKPKA
                     SRAESAEMYVLATGFRGAEAGISGQSFPRRRESSNP"
     gene            14678..16108
                     /locus_tag="VFY74_12285"
     CDS             14678..16108
                     /locus_tag="VFY74_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5932505.1"
                     /translation="MPRHFVILIVAVALFMEQMDSTVIATSLPAIAADLGEDPVALKL
                     ALTSYLLSLAVFIPLSGWVADRFGARTVFRAAIAVFTLGSAACGFAQGLPDFVAFRIL
                     QGIGGAMMVPVGRLVILRSVPKVELISALAWLAVPALMGPVIGPPLGGFISTFASWRW
                     IFWINIPIGIVGIILATRYIANIREEKLPPLDVKGFALSSIGLAGLAFGITNIGQGLF
                     PAEIVLVLLAVGVAGCWLYVRHARATTRPLLDLNLLKIDTFYASVVGGFFFRIGLGAM
                     PFLLPLLFQLGFGLSPFQSGLLTFASAVGAVAMRTAAAPIIHRFGFKPILVTNAVVSA
                     LFVAAVALFTAGTPHAIVLAVLLAGGFFKSLQFTAINSIAYADIDDAAMSRATSFASV
                     VQQLSLSAGVAMGALVLEIERMGRPDTRVVAEDFPLAFLLVGAIAASSALIFMRLPKG
                     AGASLSARTRPVGADRETAPEPAPFT"
     gene            complement(16277..17506)
                     /locus_tag="VFY74_12290"
     CDS             complement(16277..17506)
                     /locus_tag="VFY74_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5932506.1"
                     /translation="MASRGVEPKRSETGDCAGGDGPAQAATGVRPLAPADGLKVTAEA
                     KTDANAQAPASAPSIYAALDLGTNNCRLLVAKPSRRGFLVIDAFSRIIRLGEGVLTSG
                     RLSDAAMRRTIDALKVCADKMKRRGVTRSRLIATEACRIAANAPEFIARARAETGLGI
                     EIVTQEIEAKLAVSGCASLIDRNADFTLVFDIGGGSSELIWLDLSRLGRPWRRTLHDR
                     VDMQSCIAAWTSLPIGVVNLAERHGGRDVTPENYEAMVADVMAGMKEFETEHRFGERI
                     AGNHAHFLGTSGTVTTISGIHLKLPFYERSRVDGAWLSAGEVRSVSSDLIAMTYAERA
                     AQPCIGHERADLVLAGCAILEALLRVWPCQRLRVADRGLREGILATLMAEDGHDRQSS
                     QQARHHRQGQGARPHRR"
     gene            17750..17825
                     /locus_tag="VFY74_12295"
     tRNA            17750..17825
                     /locus_tag="VFY74_12295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17783..17785,aa:Arg,seq:tcg)
     gene            complement(18143..18424)
                     /locus_tag="VFY74_12300"
     CDS             complement(18143..18424)
                     /locus_tag="VFY74_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX5932507.1"
                     /translation="MQYDDAYEGNHRIVAVNENYTFTVVLEPHEGGFLVHVPALPEVV
                     TGGETEEEALAMARDAIALVLESRRERQEEIPLEIGKARVREGTVSVAA"
     gene            complement(18447..21239)
                     /locus_tag="VFY74_12305"
     CDS             complement(18447..21239)
                     /locus_tag="VFY74_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5932508.1"
                     /translation="MQKASDKNREHAGLSLVAIASGLAQEMNPNRPRTAGLTSSLERD
                     WGFDSLSRAELLLRVERAFSVRLPDHLLGEAETLEDLLPALAGASSLPTLDVTRREPI
                     EESAVAPAPADLGTLTEVLDWHARQHGERVHVTLWHDLGAETKLTYRELAERARIVAK
                     GLMRSGLEPGERVAIMLPTGFDFFAAFFGVLYAGGVPVPIYPPARPSQLEEHLNRQAG
                     ILRNAGAALLIAPPAALAIARLVKLQVESLRDVLSIEALNQGTASDGRDVPPADIALI
                     QYTSGSTGDPKGVVLSQANVLANIRALGGAMDASPSDVFVSWLPLYHDMGLIGAWLGS
                     LYYAAPLVLMSPLTFLVRPEQWLWAIHHHRATLSASPNFGYGLCLNKIDDKAIEGLDL
                     GSLRMVFNGAEAVSPDIIRQFTARFARYGFKPEALVPVYGLAENAVGLAFPPRVRAPL
                     FDRVDRAALTGRARAVPAAADDPTALEMVACGHPLPGHEIRIVGPTGELGEREEGRLQ
                     WRGPSATKGYFDNPSKNRELFDGDWLNSGDRAYIAGGDVYITGRSKDIIIRAGRNIYP
                     AEIEEAAGDVPGVRKGCVVAFGSPDQARGTERVIVLAETRETGAAALDSLRKMIEDAV
                     TPLLDASPDEIVLVPPHTIPKTSSGKLRRTSARELFEHGRLLDRPQPLWWQMLRLGVT
                     GVAQQLRRALRAFGEYLYAAWWWTVIVLAGAFLFVTVPLLAGPARRWALVRKTSQAVL
                     RLTGIKVEMTGSWPQEKDVIIVANHASYIDALILAAVLPGEPAFIAKKELERQVFAGT
                     FLKQLGALFVDRADPEGGVEDANKALEAARAGRMLVFLPEGTFTRAPGLLPFRLGAFV
                     IAARESLKVLPVTLRGTRSVLRSGHWFPRRGTVAIRVGSLVIPEAQDFAAAIRLRDEV
                     RAEILANCGEPDAMAT"
     gene            21359..21694
                     /locus_tag="VFY74_12310"
     CDS             21359..21694
                     /locus_tag="VFY74_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932509.1"
                     /translation="MRKFNLGGWLRIGIVLSVIWIVIGGSFGWNHAYDGVDANFKQCV
                     IAMKSAADLQACRDARNNALAVPRGVSAAIVAFGPLLVAWLALYGLVLLVRWIRRGFQ
                     ASRSAQPPA"
     gene            21761..22189
                     /locus_tag="VFY74_12315"
     CDS             21761..22189
                     /locus_tag="VFY74_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932510.1"
                     /translation="MMQDEVHTEDPHGFSAKLRDFGGIARDISHSALRETLSWSAYQW
                     TVLALLTAIFLLVALSYGGIRAELAVLKQNAGASTEDRTAIEAELGKQTSELKSGLTQ
                     ALADMKSALQADIAAMRTKLDALRQAPRPSGPKPPRQRQQ"
     gene            22270..23244
                     /locus_tag="VFY74_12320"
     CDS             22270..23244
                     /locus_tag="VFY74_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932511.1"
                     /translation="MTILKRLTSPMRIRLLAIIIPASVFTALLNHRLTDAPNIDDVSQ
                     NLRAAYNLAYNGIFSKDAELRRPDNYREPLPVAALALYIKMDPRLSSGQSNISINRSS
                     ALLAVKEHNLFWAFLCLVGLGAIVFASVRPPWLALIAVVAADTLTYVYFLKRDEIINR
                     TLTEIQAATLLVWFAFFLIRALETKKAAWFAAAGILLGGLALTKAAFLYVGIGLIAAI
                     ALAYGVWPPPQWRRLRVFALVGVMTASMAVVVLPWMVRNFVQFESFEISQRGGLALMS
                     RAYYKMNDTEFKGAFYHFARGGLRENLGSYLGYSPRDLEVMGRLRRLK"
     gene            complement(23641..23829)
                     /locus_tag="VFY74_12325"
     CDS             complement(23641..23829)
                     /locus_tag="VFY74_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932512.1"
                     /translation="MSPSDERLEHLIGRSTLAVAYFKSLRPHQWLKNLLVFLPMLAAH
                     QLTADTFVQSFLAIEIQS"
     gene            24101..24174
                     /locus_tag="VFY74_12330"
     tRNA            24101..24174
                     /locus_tag="VFY74_12330"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24133..24135,aa:Gln,seq:ctg)
BASE COUNT         4343 a         7881 c         7867 g         4362 t
ORIGIN      
        1 ccaatagaac tgccccggct gaaaagcttt gaagtcgtcg ctggaaggct ggccgttctg
       61 ggcgagtgaa cgaaggatgg gaaaaggctg accggcacaa agtccgacgg ttgagcgccg
      121 ggtgagcccc cgcaggctct cgatgcctac ctcccgtcgc gtgacgatcc tacgcccgcc
      181 acaagaatgt caacgacgcc tctccaccgt ccttaccgag acgagaatgg ctcgcctgat
      241 ggtgtttgac gcggaggctg tgtatcggca gcaagatcct tcacgaaaac aatcttgccg
      301 tcgcccggtg cgtagaaatc cggcagccgc gctccctcgc gaaagcccat gcgctcatag
      361 aagccgcggg tgtccgcata gcgatccgaa gaagacgtat ccgcgtagat gtgtttggcg
      421 ctcgctttga ccatggcggc ctcggcgcgc gcatagacct gggcgccgag ccctttcttc
      481 tgctcttccg gcgcaatggc gatccagaag agctcgaacg agccttgcgt cccgtggagc
      541 ggaccgtaac aggcaaagcc cacgagatta gcgccgcgct ctgccaacac gaagctgtag
      601 cccgagcgga tacccttggt gaggcgctcg gtgacgagat cggaggcggt ctcgacctcc
      661 gcggcgttga agaaacccgt gctcgccacg agcgaccgca cccgcccgac gtcgtcggcc
      721 atcacggcgc ttcgccaatc gagtccgtcg cgagcggtgt tcctcgaagg ccccgacact
      781 ttggcaagtg ccggcgccgc aagccgcgcg gcggagtggc ctgcaaccaa cccagcgaag
      841 aaattccggg cgttgctgcc gagggtacgc acgcggtacc cgccctcctg caccacgagc
      901 gtcgggaaac cctgctcgcc gatcatgcgc ccgagctgtt cgaaatcctt tgcccgattg
      961 gaccacgtgc cggtcggatc gccgcgcgcg gtgtcgaagc cgagtgagac caggagatag
     1021 gcgggggcaa agcgtttcac gcgccgcaag ccttcagcga cggcgttccg atgctgctcc
     1081 ggagtgatgt gctcaggcag cggaatgttg agattgaagc cggcccccgg accgatccca
     1141 ctctcgtcgc ggaatccggt gaaataggga taggcgaagc tcggatgggc gtggatcgac
     1201 acggtgagca cgtcggcccg gttgtagaag atctcctgct ggccgttgcc gtggtgatag
     1261 tcgatatcga gaatcgccac cttgccgtag cgcgcaagca gatttgccgc gatcgccgcg
     1321 ttgttgaagt agcagaaacc gccgaagctc cgcgtctcgg cgtggtgtcc cggaggccgc
     1381 accagcgcgt aggcaagcgg cgcgccctga agcagcttct ccgccgcggt cagcgcgcag
     1441 tccaccgccg agcgcgcggc aaggtatgcg ttctggttga ggggcgtgaa agtgtcgatg
     1501 cagtaatagc cggcgaggac cgtttcgtcc ttgggcgccc gcgccggatt gcgcagcggg
     1561 aagacgtaag ggtagatcga tttcttgggg ccggcgatca ggcaggcctt gcggatgtag
     1621 tcgaccagcc tgctgtcgtg cacggcgcgg atatgccggt cggagtagcg cttggccggc
     1681 accttctcga ccagtcccga aggatcgagc tcggccatga tcgtgcggat gcggaccggc
     1741 gcctcgaaat agtccctgtc gaggacgtga tggagcgtgt gctcgtcgtt cagcacgagc
     1801 gcgatgcgcg gcgccgaagc cgcgcgcatc tctcctctca gcgcccgccg cgtcttgaca
     1861 tagcgcggct cgcgcaggcg gacgggatcg tccttgatcg agccgacgac catctgcacg
     1921 taatccggcg gacagttgta ttttcgctcg aggatcgcgc gcacgacctt gcgtaccttg
     1981 tcacggctcg gcagatcgcc ggcgccaagc ggatcgagaa cgagataggg cggatcggtc
     2041 gtgcccgccc gcaccggcgt ctcgtaggcc gtgttcacga tcggccgcgc gccgtagcgc
     2101 tcgtagaatt tcagccgggc cgcattggcg gctctgacct ccgtattggg gctcagcgcg
     2161 ggatcgtcgg gcaggcattc gaaatagagc tgggtaccga gcgcccgtgc ttcctcgcga
     2221 acgcgttcgt agagcgccgc cccgaacccc gcccccatgc ggccgggcgc tgtcgagatg
     2281 atttccagat acgagaagcc gatatccggc gcatgaagca gaagcgccac gccgagcgtc
     2341 tggtcgcgct cattctcggc cacgaacagc cgcgacacga atttgtgctt cagcggattg
     2401 gcgaactgat cgggcagctt ggcgatgtcc tcctctggaa tcgccttaaa ctgctggcgc
     2461 atgatccttt gcgccgcctc gatggccgag cggttcaccg ccgtcgtggc atcggccact
     2521 ttgcggatgc gaagcatggg tcagatgcga agcatgtcaa gccgtggctt gcaagcgccc
     2581 gagtggcgcc tcgatgacgc gggcgatcaa cgcgtcgtaa tcgatgccgg cctcgagcgc
     2641 cgaagccgcg aacccggcgt cggcgctgag gcaggggttc acattgactt cgaggatgaa
     2701 gggcgcgccg gtccggtcga cgcggaaatc aacccgcgca taaccgtcga gagcgaaaag
     2761 gctccagcag gcaagcccga gccgcgtcag gtcggcggca agctcgggat catcggtctc
     2821 caggccgaag cgccgcggcg ttccgacata gggctggctt cccggcgtcc atttcgcgtc
     2881 gtagtcggcg atcaacggct cgcctcgccc aaatccctgg aacagcgtct cctggatcgg
     2941 gagcacctcg acgcgctgag gtctctcaat catcgcaacc gcgaattcgc ggccatcgat
     3001 gtaggcttcc gcgaaatgct ccgttcgcca tcgcagcgtc cgttcgacga ttgcgcgcgc
     3061 cgcctcggcg ccgcgcatga cagacgcggg ttcgaggccg agcgagccat gttcgaacac
     3121 gggcttcacg atcacgcgcg cctcaggatc gagccccgtc ccgtcctgcg accactccgg
     3181 cgtgggcaag ccggcatggg cgagcctgag cttcgtgccg atcttggaga gcgtgtcgag
     3241 gcacgcgctc gtcctcgcgc cggtataggg aagcgcaagc gcatcgagcc gggcaggcac
     3301 catcggcgca agccggcagt cgccgcctac cgcctcgacc aggttgaaca caaggagcgg
     3361 ccggcgagag gccagcgcct cgagaccgct taagtcgggc ccaagcccga cgatctccgt
     3421 cgcgtagccg agccgcgcga tggcttcggc caccgcttcg gcggcaatga tcgtgtcgat
     3481 ctcatcaggg cggctctcgg ttgccgcatg cagaaccggg acgaagcccg cgcttccgtg
     3541 catcaggcgc gcttccgccg aggctgctgc ttgctccggg cgagcccgta gcgggcaaga
     3601 ccggcgtcga gaatgaggcc cataagagac gtaaagtcga tcccattctg ggcggcgagg
     3661 atcggcaggt ctgagtgcga cggattgagc ccggcaagcg gattggcttc gaggaagtgc
     3721 gggtcgccct tcgcgtcgga gcggaaatcg atccgcgccg cgtcccggca ttggagcgcg
     3781 cggtaggcct gaagcgcccg cgtgcccgcc agccgcgcct ccgcgtccgt agcaggcgcg
     3841 taggtcacga gctcctcgca cagctcctta ttgtggagcg agtagacatt ggcctcggca
     3901 ttcggcttga gcaggatttc gcagacgccg agcacgcggg ccttgtcgcc attgccgacg
     3961 atgccgaccg tgaactcgcg gccgggaagg tattgctcga ctagcgccgg ctgccggtag
     4021 cgggcgatga cgtgggcgac cgcggtttca agcgcgcctt tcgaatccac gagcgacgcc
     4081 gcctcgcagc ctttgccggt gccttcggcc aatggcttga cgaaggccgg gaagtcgacc
     4141 cagtcaacaa gctcgttcgc gctgcgctcg gcaacggcaa aggacggcgt cggcacgccg
     4201 gcgtcgcgca cgaggcgctt cgttacggcc ttgtcgagag ccgccgcggc cgtgagcggg
     4261 tcggaaaaga gataaggctg atcgaacagc tcgcagagag cgggcacttg cgcctcgcgc
     4321 gagcgtcccc gcacaccttc ggcgatggag aacacgatat cgtagcgctc gcctgcgacg
     4381 agacgggccg cgagcgcctg cccccgcccg acgcggatcg gctcgcagcc gagcgcctcc
     4441 aacgcgcccg ccaaggcatc gatggtttcg accgagtcga actcggcgac ctcctcctcg
     4501 gagaagccga gcgcgcgata gtcgttgcgc aggtcgtaga cgagagcgac acgcatcgca
     4561 cttatccggc gccgggggtg cggccgattc cgacagtgcc ctcggggtcg gtataacgat
     4621 agatattgcc ctcgaagttg cgcaacagga ggtcgtcgcc ttcgcggccg gcgacatagt
     4681 cgggaaggag cggaatcttg ccgccgccac ccggcgcatc gaccacatag gtcggcacgg
     4741 cgtagcccgt ggtgtggccg cgcaggcccc ggatgagttc gagccccttg tcgacgctgg
     4801 tgcggaaatg ggccgagccc gagatcgggt cgcattggta gaggtagtag ggcctgaccc
     4861 ggcgcatcag gagaccatgg aacagacttt tgagcgtctc gacctcgtcg ttgataccct
     4921 tgagcagcac tgtctggctg ccgagcggaa tgccggcatc ggcaagccgc gccgtcgcct
     4981 cagtgacctc gggcgtcaac tcggatgggt gggtgaagtg gaggctcatc cagagcggat
     5041 gatgcttctt gagcacacgg atgagcgcac gcgtcacccg catcggcagc acgaccggca
     5101 ccttggtgcc gagcctgacg aactcgacat gcttgatcgc ccggaggcgc ccgagcagat
     5161 agtcgagccg ctcgtccgag agcgtcagcg gatcgccgcc ggaaatcagc acgtcgcgga
     5221 tctcgggatg cgacgcgata tagttgagcg cctcctccca ttgcggccgg ctgaaggtgt
     5281 actcgccgcc cgcctcgccg accatgcgcg agcgcgtgca gtagcggcaa tacgtggagc
     5341 agaagcccgt ggcgaggaac agcacccggt cgggatagcg gtgcaccagc cccggaacgg
     5401 tcgtatgtcc gtcctcgccg agcggatcgt cggcctcgcc cggggtcttg agatattcgt
     5461 cgccgaccgg aatgtgggtg cggcggaccg gctcggccgc gtcctcccgc gccatgaggc
     5521 tcgcatagta gggcgtgatg ccgagcggca gcgagccctg atggcggatc acggcctcgc
     5581 gctcgtcctc ggacaggacg aagatgcgct caagctcggc gagcgtgcgg atgcgcgcgc
     5641 gcgtctgcca gcgccaatcg ttccaagtgg cggtatccac ctcggggtaa tagcgcttgc
     5701 ggaaggcgcg cgagagcggc ccgatgggga agcgctccat gcgcaccacg acggcgtcga
     5761 gccgcggcgc cttcgccggt aaggccggcg cgggcgatag agcgttgatc ggggaatgag
     5821 gtttgatgag gagtcgggat gtcgccttgg ggcgactggg cggctcttcg gcttcgacct
     5881 gaagcaactt catgggatgt ctgtcccctt cggccttgcg acggatatgc cccggcgcca
     5941 accgccggcc ctggtccgtc aaaagcgcga gtcatttcag cgagctggtt cttaatttca
     6001 agtattaacg tgatcgcgcc cccaattttt ttcgaacgtg tccagcagga tgcgcgagcg
     6061 cgctgcaacg ctcagccagc gcgctgcagc acggcgacga agaagccgtc agtgccgtgg
     6121 tcgcgtggcg tcaggagcag cgtctcgccc gagccgtcgg cggagggcgc tggcgcctgc
     6181 cccggaagcg atctctccca cagctccggc cacggaatga gctcgaattc gggacggcgg
     6241 gcgagcaagc cgtcaatctg gtcccggttc tcagacggca ggaccgagca ggtgacatac
     6301 gcgaggcggc cgccgggctt gacgagcgcg gcgccctcct cgagcaccac cctctgctcg
     6361 ccgcgcctcg cctcgagcat cgaaagcgag agccgccatt tggcgtcggg gcgccgccgc
     6421 cacacgccag agcccgagca cggcgcgtcg atcaggacga ggtccatttt gcccgcgagt
     6481 tttcccaagg cctcgcgttc gcccgggggc agcacctgga cgttccgcgc gcctgcccgc
     6541 ttcagccgct cgaagatcgg ccggagccgc aaggcgtcgg cgtcataggc gtggagctga
     6601 ccggtattct gcatcagggc ggagagcgcc aaggtcttgc cgccggcgcc ggcgcagaga
     6661 tcgatgacct gctggcgcgg gccggcaccc gatagaaggg tggcgagctg cgagccctcg
     6721 tcctggactt cgtaccagcc tttaccgtgg ccgggttctg cttccagatg cggactgcgg
     6781 cccggtccgg acgcctgcgc ggcgcgaacg ccaaagggcg aatgcggcgt cggcccaacc
     6841 tcgaaccggc ggagcgcctt cagcaccttg tcgcgatccg cttttagcgt attcaccctg
     6901 agatcgaccg gagcgcggcg ggccaagcct gcgccctgct ccgccgcgcg agcgccgaag
     6961 gccctctcga actcaggccc gagccattcc ggataatcgc cccggatgtg agcgggagcc
     7021 gtggcgggga tctccaactt gagcccggca agctcggcct ccgacagggg ctccggcgca
     7081 tagcggctgc catcggcaag ttgggcgacc tgctccggcg tcatctgcca tcgggtgacg
     7141 aggctccgca gcacgagcgc gcgcgggctt ccctcgccca tggcatgggc cgccgaggcg
     7201 cgggtgcgca aggcatcgaa cacgagattg ccgatcgccg ccctgtcgcc cgatccggcg
     7261 acgcgatgcg acgtccccca atccttcaga gcttccgccg cgggccgctt gcgcgcctcg
     7321 atatcggcga gaatttccat cgccgcgctc gcccgcgccg ctggcgtcat caggacccgg
     7381 tgaacaggat gatgcgggcg atgacgatca cccacatgag aaggagcgcg atggcgctga
     7441 tgccgaatac gccggtgcgg cggtcgagcc ggttggacgt gacatggata taggcatgga
     7501 caagccgcga cagcacgaac agccagctca agacgagaag ggtgacatcg agggtctggc
     7561 tggcgaacgc cagcagcacc acgacgtaga acagaaccgg cagctcgagc tggttatggt
     7621 aggcgttcga aatctggaga acgcgcgccg gccaatgcgg ctcgcggagc ctgattttct
     7681 ccggatcgac ctcgccggcc tgaatggcgc gcacgcggag cgccgccatc caaaacagga
     7741 gcgtaaaggt gagcccgacc tggacgaaca tcggcatcaa aatcgcgtgt atcgacatgg
     7801 gcccctctcc ttctttagcg ccatgcccgg cggctcgcgc tgaagcgagc tagagcaatg
     7861 tccaggcgat taggcaagcg cgtcgtgacc ttggggcctc gcttccgcga caggctcggt
     7921 ttcttgccgg ttgcttcacc ggcggccacg atccgcgacg ttcgtaggca gaggcaaccg
     7981 gctgattgcg ttgccacatt cccgagccaa tcatggacag aagttcaatt gacggccccc
     8041 gggagccgcc tctaaaccta ccgccaaaga tcgaaaccca gcgtccaccg tgaggaggaa
     8101 ttccatgcgc cgtcgcgccc tttatgccgt ttcctgcgcc gccattgccg gcgcgctcgc
     8161 tttcgccgct tttcctcgcc ccggacacgc cgcagacaac ccgtgtttca ccggggaggg
     8221 gtcgatcaaa gaaatcctcg acgggtgcgg cgcctatatc ggctcgggcg cgaccgacaa
     8281 ggatcagctc gtccgggccc acgccgtgcg tgccatggcc ttctccgcga tccgcgatct
     8341 cgattcggcg atcgccgagc tcgacgaggc catcaaggtc gatgcgatta gggcgaattc
     8401 ctatttcatg cgggccgcgg cgcatgaggc caagaagaac catgagaccg cgctgaaaga
     8461 tctggacgag gccatccggc tcgatgccaa gcaggccgac tactacctgc tgcgcgggat
     8521 tatttaccgc gacaagggcg atctcgatcg ggcgctcgtc gatttcgacg agaaggtcaa
     8581 gctcgagcca aacgcagcgc tcggctactc caaacgcggc gatctctacc ggcagcgcaa
     8641 ggaatacgat ctcgccatcg ccgacttcga tcaggtgatc aagatcgatg acaaggacgc
     8701 caagggttat atcgatcgcg gctgggtctt cgtgctcaag aacgatttgg acaaggccgg
     8761 ggccgatttc gacaacgcgc tcaagctgca tgagaacgag ccttccgctc tcgtcggccg
     8821 cggcctggtg aagagccgta aaggccagcc caccgacggc agcgccgacc tcgcgctcgc
     8881 gcagaggctg gagcccggca ttttcgacga aatcaagaag ctcggcgtcg agtaggcgct
     8941 gggtttgtca ccgcaacctg tcccgctctc cccttgggga gagcacacgc gcctcttggc
     9001 gcgtgatgag gggggttcca cgagtgtctg ccgagcgaaa gaaagttccc cctcaccctt
     9061 cccgccatgc gacttgcgca gagctacgct cgcgcaagat cgcggcggaa agccctctcc
     9121 ctcaagggag agggggcccg gggtgtgcgg tcatgacaga caacggacgg gccgtgaaag
     9181 tctttacccc tcgcgcgggt agttgggcga ctcgcgcgtg atggtcacgt cgtggacgtg
     9241 gctctcgcgg aagctcgccg ccgagatgcg gatgaattcg gcgcgcgcct gaaactcggc
     9301 aagcgtccgc gccccggtat agcccatcga ggcgcgcaag cccccgatga gctggtgcag
     9361 gacgctcgag acgggcccct tgtagggaac ctgtccctcg atgccctccg gcacgagctt
     9421 gagcgcgtcc ctgatctcct gctggaagta gcggtcggcc gagccgcgcg ccatggcgcc
     9481 gatcgagccc atgccgcgat aggccttgta ggagcgcccc tgatagagaa acacctcgcc
     9541 cgggctttcg tcggtgcctg cgagcagaga gccgagcatc acgcaatcgg cgccggccgc
     9601 gagcgccttg gcgagatcgc ccgaatatct gatcccaccg tccgagatca ccgggatatc
     9661 gctcttcttc gccacctcga ccgcctcgag caacgccgtg aattgcggca cgccgacgcc
     9721 ggcgaccacg cgcgtggtgc agatcgagcc cggaccgatg ccgaccttga cggcatcggc
     9781 gccggcatcg atcagcgcct tggtgccgtc cgccgtcgcc acgttgccgc caacaacttg
     9841 cacggcattg gactgacgct tgatgcggcg cacctggtcg agcacgagcg ccgaatggcc
     9901 atgcgcggta tcgacgacga tcacatcggc gcctgcatcg atcaaccgct cggcgcggtc
     9961 gaatccggaa tcgcccacgc tcgtcgccgc cgcgactcgc aagcgcccct gctcgtcctt
    10021 gcaggcattg ggatgggctc ttgccttctc gatgtccttg accgtgatca gtccgatgca
    10081 gcgataggcg tcgtcgacca caagcagctt ctcgatgcga tgctggtgca agagccgctt
    10141 tgcctcttcc agcccgacat tttccctgac cgtgacgagc ttgtccttgg tcatcagctc
    10201 cgcgacccgc tggttcgggt tggtcgcaaa ccgcacgtcg cgattggtca ggatgcccgc
    10261 gagcctcccc tcgcgtccgc cattgccggc gggctcgacc accgggatgc ccgagatctg
    10321 atggtcgacc atgagcttga gcgcgtcggc gagggtggcg ccgggatgga tggtcaccgg
    10381 gttcaccacc atgcccgact cgaatttctt cacctgcgcg acctgggcgg cctgagccgc
    10441 gggatcgaga ttgcgatgga tcacaccgat gccgcccgct tgggccatgg cgatggcaag
    10501 cttcgcctcg gtcaccgtgt ccatggcgga ggagacgacc gggatgtgca gggcgatgga
    10561 gcgggtaatc cgggaagaga cgtcggcctc gttcgggagg acgttggagg ccgagggcct
    10621 gatcaggacg tcgtcgaagg tgagcgcaat gccgctgtcg gggaattgct tccgcttttc
    10681 aggggtttgc ccttcgggca tcggccaacc tcatgcgcgc gggcgaccgc agcgtgtgct
    10741 gcaacaagcc accggttggc ggcctccttt agcactttga tccgggtttg ggaaggcaat
    10801 ggcgcgcgac gtcgatcctt tatcctttcc gtcccgatct gagcacttgg cgaagccggg
    10861 gtgcggcgaa agcgatgaag ctctcgacct tggcctgggg caggcgatct tcgcgatgga
    10921 aaaggttgac gggtatctct tcgggctcga acgcctccag aatgcgctgc aacgccccgt
    10981 tggcgattga ggcttccccc tgatgggaaa gcaccctcac gatcccaagc cccgccctgg
    11041 ctgcatcgat ggccgcgtcc gccgagttga cgaccaatct cgggctgacc gtgacccgaa
    11101 gctctctctt gccgcgctca ccgaaagtcc aacgctcggc gtgggtgaaa gtgttgaagc
    11161 tgatgcattg gtggccggca agctcggaag gtgaggccgg tgttccatgc tgcgcaagat
    11221 aggtggggct cgcgcaggcg ataaggcgaa ttgcgccgac gcgcgttgcg cggagcgtcg
    11281 agtccgggag attgccgatg cgcacgccga tatcgatgcc ctcttccacc agatcgacga
    11341 cccggtcgac cagaagcatg cgcgccgcaa cgcgcgggaa cgcgttcaag aacgcagcaa
    11401 caatcgggag cacatgcagc ctgccgaaca cgacgggtgc ggtgagcgca agttcgcccc
    11461 gaggctcggc atgttccccg gtcaagcgac gtccggcgtc atcgagctca tcgagcacct
    11521 gtcggcacgt cgcggcgtaa gcgcggccgg actcggtcag cgttagccgc cgcgtcgttc
    11581 gcgtgatcag gcgcgtgtcg agctcttctt caagcgcctt caaatgccgg ctgacggtag
    11641 agagaggcat tccaagcttg cgccccgccg cggacaggct gccggcttcg gcgacggtaa
    11701 cgaacaggcg aagtgcggca agacggtcca tgggattatc tcaagaaatg gaagaatgag
    11761 ttgcgaaaat aacggatatt cctagcaggg ggaatggggc atgacaggcc cgggctggcg
    11821 cagagcccag ccgaagagaa agggaaaagc gcatggccca gttgaagctt cacgacctcg
    11881 aactgtcggg caactgctac aaggtccgtt tgttttgctc gctgctcggg ctcaagctcg
    11941 acctcgtgcc ggtcgatttt ctcgccggcg cgcataagaa gagcccactg atcgagaaga
    12001 acccgttcgg cgaaatcccg atcctcgaag agggcgacgt tgtcttgcgc gattctcagg
    12061 cgatcctcgt ctacctcgcg cggaaatatg gaggggagca gtggcttccg accgatccgg
    12121 tgtccatggg acgcgtcgtc gcgtggttga tggtcgccga gaacgaggtc gcgcgcgggc
    12181 cgaatgacgc aaggctccac gacaagttcg gtttcaagct cgatgcctct gcggcgctgg
    12241 agaaggccaa gcgcatcttc ggcctgatgg aatcgcatct ggccaagaac gattggcttg
    12301 cgcttgatcg cccgaccatc gccgacatcg cctgcatgcc ttacatggcg ctcagccacg
    12361 aaggcggact gtcgctggag ccttatcccg ccatccgcgc ctggatcagg cgcatcaagg
    12421 ccttgcccgg cttcatcacc atgccggccc tgtgacctcg cgatgtcctc ctcgacagac
    12481 gttgccttta cgctttctgt gaagcgcctg caggatcggc gcggttcgcg cggcgcctat
    12541 gcccgcatgg aggagaaggg cggcttcgcc acgaagatcg acgacggcct cgctgccttc
    12601 atcgccaaca agcgatcctt ctatttcgcc accgccaacc gcaacggaca gccttatatc
    12661 cagcatcgcg gcggcccgaa ggggttcttg cgcgttctcg atccgacgac gctcgccttc
    12721 gcggatttcg gcggcaaccg gcaatacatc acgaccggga acctcgccga caatccgcgc
    12781 gcgatgctgt tcctcatgga ttacacgcag cgcaggcggg ttaagatctg gggaacggcg
    12841 cgcgccgtcg aggacgatgc tgagctaatc gcgaagctgt ttcccgacgc ctacaaggct
    12901 cgggctgagc aagccattgt cttcaccgtc gcggcttggg acgcgaattg cccgcagcac
    12961 atcccgcaaa tgctgtttgc cgaggatgtc gccgaggctt tcgcggaacg cgaccggcgt
    13021 atcagcgcgc ttgaagccga gatcgccctc ctgcgcgctg ctcccgcggg gatggagaat
    13081 ctttagcctc ggccgcgggc tcggcgaaca gccagccgaa gaagtatgac gcggcgaccg
    13141 cgccgagaag ctcggccacg atgaagcccg acgcgtcggc gggagcgatg cccgaaaacg
    13201 tgtcgctgag cgcgcgcgcg atggtcactg ccggattggc gaaggaggtg gacgacgtga
    13261 accaatacgc cgagccgata tagaggccga ccgccgcagg caacgcacca ggcctgaagc
    13321 gcagacagcc gagaatggtt gcgaccagcc caaacgtggc gatgaattcg ccgagccact
    13381 ggccgaagcc ggtccgcgcg ttgcctgaaa tctgaacggg cgcgaggtcg aacatcagat
    13441 gcgccgccac agccccaagg cacccggcta cgacctgcgc cgcgacatag aggagcgccg
    13501 tccgggattc gatctcgccg cgaagcagca tgacgagcgt cacggcgggg ttgaactgcg
    13561 cgccggaaac cgaaacgaag atggtgatga tgacgacgag gatcgcggcc gtggcgaccg
    13621 tattgcagag cagcgcgagc gcgacatttc cgccggcaag catctcggcc atgatgcccg
    13681 agccgaccac cgccatcaca agaatgaacg tgccgagcgc ctccgccgac aagcgtcgcg
    13741 caagatcgat ttgcattagg cttcctgctc ccattccatt gtcattgccc ggcaggaccg
    13801 ggcaatccac ttgttccccc ggagttaggg gttactagat tcccgccttc gcgggaatga
    13861 ctgccccgaa attcccgcct ccgcgccgcg aaaaccggtg gcgagcacgt acatctcggc
    13921 ggattcggcg cgggacgcct tcggcttcac gtggcgcacc ttggcgaaag agcgattgag
    13981 gcgagccacg agctctctgc tcgcgccacc ctggagcacc ttgctgagga aggttccgcc
    14041 gggggcgaga atgctctcgg caagatcgag cgcagcctcg accaggccca tgttgcggag
    14101 atggtcggtc gccttgtgcc cggtcgccgg cgcatgcatg tctgagagca ccacatcggc
    14161 cctttctccg cccaacgcct cgccgatgcg cctcgacgca tcccaatcgg tgagatcgag
    14221 ctcgagctgg acgacgcccg cgatcggttc catcgctgca aggtcgacgg cgaccacccg
    14281 acccttcccc gccatgagtc ccacgcgctg cgcggcgatc tggctccatc ctcccggcac
    14341 ggcgcccaaa tcgacgacgg tcatgcccgg cctcagcaaa tggtgcttgt cgtcgatctc
    14401 gatgagcttg aaggcggcgc ggctgcgata gcccctgcgc ctcgcctcct cgacataggg
    14461 atcgttgagc tggcgctgga gccagcgggt cgaggcgagc ttgcgtccct tggcggtgcg
    14521 gaccctgacc gcgagcttgc ggtgtccgcc ctgccgctcg ccgccctgcc cttttcccgg
    14581 cctcaccatc tgttaaatgc ccgatcttgc ggcccgagct tgacgctcag gcgcgctttg
    14641 tcccaactcc gcatcgtcgc ccgccaacac ctgccacatg ccacgccatt tcgtcatcct
    14701 catcgtcgcc gtcgcgctct tcatggagca gatggattcg acggtgatcg cgacctcttt
    14761 gccggcgatc gccgccgatc tcggcgagga cccggtcgcg ctcaagctcg cgctcacctc
    14821 atatctgctg tcgcttgccg ttttcatacc cttgagtggc tgggttgctg accgtttcgg
    14881 ggcccgcacg gtgttccgcg ccgccatcgc cgtcttcacg ctcggctcgg cggcttgcgg
    14941 cttcgcccag gggcttccgg atttcgtcgc cttccgcatc cttcagggca tcggcggcgc
    15001 catgatggtg ccggtcggcc gtctcgtcat cttgcgctcg gtgccgaaag tcgagctgat
    15061 ctccgcgctc gcgtggctcg cggtgcctgc gctcatgggg cctgtcatcg ggccgccgct
    15121 cggcggcttc atctccacgt tcgcctcatg gcgttggatc ttctggatca acatcccgat
    15181 cgggatcgtc ggcatcattc tcgccacgcg ttacatcgcc aatattcgtg aggagaagct
    15241 gccgccgctc gacgtcaagg gtttcgcgct ttccagcatc ggattggcgg gcctcgcctt
    15301 cggcatcacc aatatcgggc agggcctgtt tccggcggag atcgtgctgg tgctgctcgc
    15361 ggtcggtgtc gccggctgct ggctctatgt gcgccacgcg cgcgcgacga caagacctct
    15421 gctcgatctc aacctgctca agatcgatac gttctacgcg agtgtcgtcg gcggcttctt
    15481 cttccgtatc gggttgggag ccatgccgtt cctcttgccc ctgctgttcc agctcggttt
    15541 tggcctgagc cccttccaat ccggattgct caccttcgcc agcgccgtcg gcgccgtcgc
    15601 catgaggacc gccgcagcgc cgatcattca ccgcttcggc ttcaagccga tcttggtgac
    15661 caacgcggtt gtgagcgccc tgttcgttgc cgccgtcgcg ctgttcaccg ctggaacgcc
    15721 tcacgccatc gtgctcgccg tgctgcttgc cggcgggttc ttcaagtcgc tgcaattcac
    15781 cgcgatcaat tccatcgcct atgccgatat cgacgacgcc gccatgagcc gggcgacgag
    15841 cttcgcgagc gtcgtgcagc agctgtcgct ctcggcgggc gtcgccatgg gcgcgctcgt
    15901 cctcgagatc gagcgcatgg gccggcccga cacacgcgtc gtggcggagg attttccgct
    15961 cgccttcctt ctcgtcgggg cgatcgctgc aagttcggcg cttatcttca tgcgcctgcc
    16021 aaagggcgcc ggcgcgagcc tgtccgccag aacgcgcccg gtcggggccg atcgggaaac
    16081 cgccccggag cccgcccctt tcacttgagc ctcaagtcta gatgctatcg gcttcgcgcc
    16141 gcaccttctt ctaacctccc cctggtgggg aggtcggcgc gcggtttagc gcgccgggtg
    16201 ggggcgtagc gccaggctgc aaacgtgtgg tgcatactct tctaacctcc ccctcggggg
    16261 aggttagcaa atgagcctag cggcggtgcg ggcgcgcgcc ctggccttgg cgatggtgcc
    16321 tcgcctgctg cgagctttgc cggtcatgac cgtcctcggc catgagcgtg gcgaggatgc
    16381 cctcgcgcaa gccccggtcg gccaccctga gccgctggca aggccagact ctgagcaacg
    16441 cttcgaggat ggcgcagccc gcgaggacga gatcggcgcg ctcatgcccg atacagggct
    16501 gggcggcgcg ctcggcatag gtcatggcga tgaggtcgct cgacacggag cgcacttcgc
    16561 ccgcgctgag ccaggcgccg tccacgcgcg agcgctcata gaacggaagc ttgaggtgaa
    16621 tgcccgagat cgtcgtgacc gtgcccgagg tgccgagaaa atgcgcgtgg ttgccggcga
    16681 tcctctcgcc gaaacgatgc tcggtctcga actccttcat gcccgccatc acgtcggcca
    16741 ccatggcctc gtaattttca ggcgtgacgt cgcggccgcc gtggcgctcg gcaagattga
    16801 ccacaccgat cgggagcgag gtccaggcgg caatgcagct ctgcatgtcg acccggtcgt
    16861 gcagcgtgcg ccgccaaggc cgtcccagtc gcgaaaggtc gagccagatc agctccgagc
    16921 tgccgccgcc gatatcgaac acgagcgtga agtcggcatt gcgatcgatc agcgaggcgc
    16981 agcccgacac cgcgagcttg gcctcaatct cctgggtcac gatctcgatg ccgagcccgg
    17041 tctcggcgcg cgcccgcgcg ataaactccg gagcattcgc agcgatgcgg caggcctcgg
    17101 tcgcaatcaa ccgcgagcgg gtcacgccgc ggcgtttcat cttgtcggcg cagaccttca
    17161 gggcatcgat ggttcggcgc attgcggcgt cagacagccg cccggatgtc agcacgcctt
    17221 cgccgagccg gatgatgcgg gagaacgcgt cgatgacgag aaagccccgg cgcgaaggct
    17281 tggcgacgag aagccggcaa ttgttggtgc cgagatcaag cgcagcgtaa atggaaggcg
    17341 cggacgccgg cgcttgcgcg ttcgcgtcag tcttagcctc ggccgtcacc ttgaggccgt
    17401 ctgccggcgc caacggacgc acgccggttg cggcttgcgc cgggccatct ccgcccgcgc
    17461 agtcgccggt ctcgcttcgc ttaggctcga cgccgcgcga agccatggca gccgggccgg
    17521 catcgccgcg cggcccaatc acagtgtcca aacccctgac cgggacgcgc ccgcctcctt
    17581 ggaccggccg gcgcccggtg cgttggtgct atgcggccag tgtatcaagc ccccgttcga
    17641 ggtaaagcgg gttgtgctgc atgcggggcg ctatggtctt gagactcgcg gccaagcgta
    17701 gaatatatgg agaaccgatt tcgcccccgc gcctagcctc gggggcgccg cgcccgtagc
    17761 tcaacggata gagcaccaga cttcgaatct gggggttgca cgttcgagcc gtgccgggcg
    17821 caccagtttc ttgcgctcag aaacgccatc atattgagaa cggggtccta caattgacat
    17881 ttgaaatgtc gcggcagtct gctgacgccg gtgatctgct tcaaatgaac tcagcaaact
    17941 cttggggagt gaagccggct tgcctgatga tggccgcaag catccaacga ggaagctctc
    18001 tgcctttgtg gaccggtagt tgcccttctc ggatcgtccc gatgcactag gcggtgatgg
    18061 ctgccggagg aacgcacgac ctcaaaaccg ccacgctcaa gcgccctaac gacatcttgc
    18121 gccttgagcg acggcagccg tgtcatgctg ccactgagac agtgccctcg cgcacgcgcg
    18181 ccttgccaat ttctagggga atctcttcct gccgctctcg ccggctctcc aaaacaagag
    18241 caatcgcgtc ccgcgccatt gcgagggctt cctcttccgt ttctcctcct gtgacgactt
    18301 cgggaagcgc cggtacgtgc acaagaaacc cgccttcgtg cggttcaagg acgacggtga
    18361 atgtgtaatt ttcgttcacg gcgacgatcc ggtggtttcc ttcatacgcg tcatcatact
    18421 gcacacctca aaatctgcca gttaactcat gtcgccatcg cgtcgggctc gccgcaattg
    18481 gcaaggatct cggcgcggac ctcatcccgc agccggatgg cggcggcgaa gtcttgggcc
    18541 tcggggatga ccaacgaccc cacgcggatt gcgacggtgc cgcgccgggg aaaccaatgg
    18601 ccgctgcgga gaactgatcg cgtgccccgg agcgtgacag gcaagacctt caaactctca
    18661 cgcgccgcga tgacaaaagc cccgaggcgg aagggaagca ggcccggggc ccgcgtgaac
    18721 gtcccttcgg ggaggaagac aagcatgcgc ccggcgcgcg cggcctcaag cgccttgttc
    18781 gcatcctcga cgccgccttc ggggtcggcg cgatccacaa acagcgcgcc gagttgcttc
    18841 aggaacgtgc ccgcgaagac ctgccgctcg agctctttct tggcgatgaa ggccggttcg
    18901 cccgggagga ccgcggcaag gatcagcgcg tcgatatagc tcgcgtggtt ggccacgatg
    18961 atgacgtcct tctcttgcgg ccaagagccg gtcatctcga ccttgatccc ggtgagccgg
    19021 aggacggctt gcgacgtctt gcgcacgagg gcccaacgcc gggccggccc ggcgagcagc
    19081 ggaaccgtga cgaagaggaa cgcccccgcc agcacgatca cggtccacca ccaggccgca
    19141 tagagatatt cgccgaaggc gcgcagcgcc cggcgcaatt gctgcgccac gccggtaacg
    19201 ccaagccgca gcatttgcca ccatagtggc tgcggccgat ccagcaggcg gccatgctcg
    19261 aaaagctcgc gggcggaggt gcggcgcagc ttgccgctcg atgtcttggg aatggtgtga
    19321 ggcggaacga gaacgatctc gtcgggagac gcatcgagca agggcgtgac cgcgtcctcg
    19381 atcatcttgc gcaagctgtc caaggcagcg gcgccagttt cgcgcgtctc cgcgagcacg
    19441 atcacgcgtt ccgtgccgcg cgcttgatca ggactgccga aggcgacaac gcaacccttg
    19501 cggacgcccg gaacgtcgcc cgccgcttcc tcgatctcgg ccgggtagat gttccggccg
    19561 gcgcggatga tgatgtcctt ggaacggccc gtgatgtaga cgtcgccgcc ggcgatatag
    19621 gcgcggtcgc cgctgttcag ccaatcgccg tcgaagagct cgcgattctt gcttggatta
    19681 tcgaaatagc ctttcgtggc ggacggaccg cgccattgaa gccgcccctc ctcccgctcg
    19741 ccaagctcgc cggtcgggcc gacgatacga atctcgtgcc ctggaagagg atgcccgcac
    19801 gcgaccatct ccaaggcggt ggggtcgtcg gccgccgccg gcacggcccg cgcccgcccg
    19861 gtcaacgcgg cacggtcgac gcgatcgaag aggggcgcgc gaacacgcgg cgggaatgcg
    19921 agcccgaccg cattctcggc caagccgtag acggggacga gcgcttcggg cttgaagcca
    19981 tatctagcga aacgggccgt gaattggcgg atgatgtccg gcgagaccgc ttcggcgcca
    20041 ttgaagacca tgcggagcga gccgaggtcg aggccttcga tcgctttgtc gtcgatcttg
    20101 ttgaggcaga gcccgtagcc gaaattcggc gaggccgaga gcgttgcccg atgatggtgg
    20161 atggcccaca gccattgctc gggccgcacg aggaaggtga gcggcgacat gagcacgagc
    20221 ggagcggcgt aatagaggct gccgagccat gcgccgatca atcccatgtc atggtagagc
    20281 ggcagccagc tcacaaacac atcggacggg cttgcatcca tcgccccgcc cagggcgcga
    20341 atgttcgcga gcacatttgc ttggctgagc accacccctt tgggatcgcc ggtgctgccc
    20401 gaagtatatt gaatgagggc gatgtcggcg ggcggcacgt cgcgaccgtc gcttgccgtg
    20461 ccttgattca gcgcttcgat gctcaataca tcccgcagac tctcgacctg caacttgaca
    20521 agccgcgcga tcgccaatgc agccggcggg gcgatgagga gcgccgcgcc tgcgttgcga
    20581 aggatgccgg cctgccggtt gagatgctcc tcgagttgcg aagggcgtgc cggcgggtag
    20641 atcggcactg gcacgccgcc cgcgtagagc acgccgaaaa acgccgcgaa gaagtcgaag
    20701 cccgtcggca gcatgatcgc cacccgctcg ccgggctcaa gtcctgatcg catcaagccc
    20761 tttgcgacaa tgcgcgcccg ttccgcgagc tcgcgatagg tgagtttggt ctcagccccc
    20821 agatcatgcc aaagcgtgac atgaacccgc tcgccgtgct gccgcgcgtg ccagtcgagc
    20881 acttcggtga gggttccgag gtcggccggt gcgggagcaa ccgcgctctc ttcgatcggc
    20941 tcgcgccgtg tcacgtcgag ggtaggcagc gacgacgcgc cagcgagcgc cggcaacaga
    21001 tcctcgagcg tctccgcctc gcccaacaga tgatcgggca gccgcacgga aaaggcgcgc
    21061 tcgacccgaa gcaggagttc cgcgcgcgaa aggctgtcga acccccagtc gcgttcaagc
    21121 gagctggtca atcccgccgt acgcgggcgg ttcgggttca tctcctgcgc aagcccgctc
    21181 gcgatcgcaa cgagcgaaag gccggcatgt tcgcggttct tgtcggaagc tttttgcatc
    21241 gacgaccgtc ctcggcatcc cttatagacc tgccggcctc tcccttcgag ccgtgtcggg
    21301 cgtcatttgg tagctcttgg ccgcgcggcc gacgaggctc gcgtgctaag ctcggcccat
    21361 gcggaagttc aatctcggcg gctggctgcg catcggcatt gtcctttccg tcatctggat
    21421 cgtgatcggc gggagcttcg gctggaacca cgcctatgac ggggtcgacg cgaatttcaa
    21481 acaatgcgtc atcgccatga aatcggccgc cgatttgcag gcctgccgcg atgcgcgcaa
    21541 caatgcgctt gccgtgccaa ggggggtgag cgcggccatc gttgccttcg gcccgctcct
    21601 cgtggcttgg ctcgcgctgt acgggcttgt tctgctcgtt cgctggatcc ggcgcggatt
    21661 ccaagcctcg cggtcggccc aaccgccagc ctagcgccgg tcaacgcggc gcgtgctatg
    21721 ctgggcgcgc aacggtaccg acggcgcgcg agggagggtg atgatgcaag acgaggtcca
    21781 tacggaggat ccgcacgggt tttcggcgaa gctgcgtgat ttcggcggca tcgcgcgaga
    21841 catcagccat tccgctttgc gcgagacgct ctcctggagt gcctatcagt ggaccgtgct
    21901 tgctcttctc accgcgatct tcctgctggt cgccctgtcc tatggcggca tcagggccga
    21961 gcttgccgtg ctgaaacaga acgccggcgc ctcgactgag gaccggacgg ccatcgaagc
    22021 agaactcggc aagcagacgt cggagctcaa atccggcctc acccaagctc tcgccgacat
    22081 gaagtcggcg cttcaggccg atatcgccgc gatgcgcacc aagctcgatg cgctgcgcca
    22141 ggcgccgaga ccatccgggc ccaagccgcc gaggcagcgg cagcaataga cccatccgcc
    22201 gagccttgcc aatccggctt ggctgcgcgc gcaaaccttg tgggatgaaa aggacgacaa
    22261 ggtaggctaa tgacaatttt gaaacggttg accagcccaa tgcggatcag gcttctagca
    22321 attataatcc cagcttctgt atttactgct cttctcaacc atcggctgac agatgcgccc
    22381 aatattgatg atgtttctca aaatcttcga gcggcttata atcttgcata taatggcatc
    22441 ttttctaaag atgccgagct gaggcgtcct gacaattatc gcgaaccgct gccggtggca
    22501 gcgctcgccc tctacattaa aatggatccg aggctctcct ctggacaaag caacatcagc
    22561 atcaaccgga gctcagcact cctggccgtc aaggagcaca atttgttttg ggctttcttg
    22621 tgcctggttg gcctgggtgc catcgtattc gcctcggttc gcccgccttg gctggcgctg
    22681 attgcggtcg ttgcggccga cacattgacg tatgtgtact tcctaaaaag agatgaaatc
    22741 ataaatcgga cgttgacgga gatccaggca gccacgctcc tggtctggtt cgctttcttt
    22801 ttgatccgag cactggaaac gaagaaagct gcctggttcg ctgccgcagg aattctactt
    22861 gggggactgg cgcttacaaa agctgccttt ctttatgtag ggatcggcct tatcgccgcg
    22921 atcgctctcg cgtacggcgt ctggccacct ccgcagtggc gacgtctgcg tgtgttcgct
    22981 cttgttggtg tgatgacagc ctcgatggcg gtagttgttt tgccctggat ggtgcgcaac
    23041 ttcgttcagt tcgagtcatt cgagatctct cagcgtggcg ggctcgcact gatgtcccgc
    23101 gcctactaca aaatgaacga cacagaattc aaaggggcgt tctatcattt cgctagagga
    23161 gggttgcgcg aaaatttagg atcttatctt ggctattcgc cgcgcgatct tgaggtgatg
    23221 ggccgtcttc ggcggctcaa gtaaacgttc agcgagcttc gcggcgagag atttggaggc
    23281 cgaaaaagca ggccgtcccg atgatgcaat ttccttctat cgtgctgcac gggctgagcg
    23341 agtgcgattc acaaaatttt tcgccgaaca gggtgtggag catccgagca atcgcgctga
    23401 cgccgaattg caaaggcagg ccatcagcca aattatgcga accccggtac tccgcaccgc
    23461 tcgttcccaa ttttatccta gcactgattt tggcggtggt ctggactggt ggagtattgg
    23521 ccgcaacagc tagtgggcgc gcccacggcg cacgacccga agtaaaatcg ccgcatcacc
    23581 agaagagccc gtgagagcgt gtcacgagat gtcattccca tgcgaagctt gaactcacat
    23641 tcaactttga atctcgatgg caaggaatga ctgaacaaag gtgtcagcag tcaattggtg
    23701 tgctgccagc atgggcagga acaccagcag atttttgagc cattggtggg ggcgcaggct
    23761 tttgaagtat gcgaccgcca atgttgatct cccgatgagg tgctccagcc tctcgtccga
    23821 tggcgacacg atctctgagc ctctttgtgt gattgattgc caaaagccga ccagaatgct
    23881 gcagtgtcta tgtcgggtca aacggtcggt gagcggaagc cgcctgcggg acgatggtgc
    23941 acctcacccg cttaagggga ttacgctaca aagccggttc gccgatcaga agaaacttgc
    24001 gaaggccgcg atcctgcggc tagaacggcg aacctcgagc gcgagcgcgc gccccttgcg
    24061 tcttgtgtgg cccaagtttt cgcgccacat atcgcgctgg tgggggatcg tctaacggta
    24121 ggacagcgga ctctgactcc gccagtcttg gttcgaatcc aagtccccca gccaacctga
    24181 atgatctcgc aggttgacca tcagatcgaa gggcgaacgc aaggaaaact ctagcgtttt
    24241 tcctttgagc cgtaggttcg aaaagacgaa agagatgagt tcgcgctttt gctcgatttt
    24301 cgaacgctcg aaaagctcgg ctgctcgcga agccagtgaa atcaggcttt ccagtgtggc
    24361 gcggacgtga ccgtcgccct cttgatgctg ctcaatttgg tgggcgatct ctgcctgtcg
    24421 ctgtttcagt tcgcgcgcct ttttgtcgta ctc
//
LOCUS       DASUQP010000272         8875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate SMAG_U1684
            k141_1498620, whole genome shotgun sequence.
ACCESSION   DASUQP010000272 DASUQP010000000
VERSION     DASUQP010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961820
            Sequence Read Archive: SRR25158510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 15:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,439
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 15 of 41
            Pseudo Genes (incomplete)         :: 23 of 41
            Pseudo Genes (internal stop)      :: 6 of 41
            Pseudo Genes (multiple problems)  :: 3 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498620"
                     /isolate="SMAG_U1684"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /country="China"
                     /lat_lon="34.41305556 N 109.1386111 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1553)
                     /locus_tag="VFY74_12335"
     CDS             complement(84..1553)
                     /locus_tag="VFY74_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932513.1"
                     /translation="MPQDMLHDTPKGRATALVAHPDDAVAAIGGRRGARALAKAGRRE
                     RVPRVKLVKPSALGMLLKPIGVAVALILSAYALLSLGTTFYRSYYRHLDHTAPVHADK
                     SVFDIFFGDRSLPPDAVDLVIKDVDGSLRKVVASKSEADKFVNDTILMLDEERARIKQ
                     AAHEDLDRSFALAFQDREQAISAYADWYFEWKRSYVVLKETVSSAITRFFEAGKYESV
                     NEAIEADIKDYFLTNYKDQVLKPELRDQTIIKGVEQAVRHAHDSYRRVVANGDMRLQL
                     FLAKHTSHLEDIPAATPMSNIKLDWDAQKWKAPTYLMEDRAFDGVVGVGTAAAGGTVG
                     ALALGPAINGVLAQSFGQLSRRVATSLGTRIAFAQQGAVAGTVVQPMGGQVVGAAIGV
                     LIGAAVDYLANAANEAFNRESFVAANEEALDATIATWKAGLEANIDTSIDKWFDDARA
                     AVVLASNRAVDFSRISAPYGVMLRPNAKGPRGWMRASRR"
     gene            1656..2354
                     /locus_tag="VFY74_12340"
     CDS             1656..2354
                     /locus_tag="VFY74_12340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX5932514.1"
                     /translation="MGTLRKRLGFCRVEVTPLARGPETDLPLFRERRVNDPKRALELL
                     AEEARRCTRCPLYRNATQTVFGEGPADAALMLIGEQPGDQEDLAGRPFVGPAGKVLDA
                     ALEAAGIPRADIYVTNAVKHFKNEPRGKRRLHKKPDTSEIDACRWWLDHELAIVQPAV
                     AVALGATAARALMRKALPINANRGRFLPLPEGGKALITVHPSYLLRLQEERDKRREFG
                     LLVRDLRLAADAAR"
     gene            complement(2493..2756)
                     /locus_tag="VFY74_12345"
     CDS             complement(2493..2756)
                     /locus_tag="VFY74_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932515.1"
                     /translation="MDFARYRHQLLQRSEGQVELWQWERNFSQWFFDLLRNFFLVGVL
                     KFFYEKGGGAVLYYVHQFALVVMLFYCLSCADQWYVKSVRFSR"
     gene            complement(2768..3106)
                     /locus_tag="VFY74_12350"
     CDS             complement(2768..3106)
                     /locus_tag="VFY74_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5932516.1"
                     /translation="MKYIIAVIQPHRLEAIREALAGIGVQGLTVSEVRGFGRQKGQTE
                     IYRGAEYVMQYVPKVKLEIAVDDAIADAVVEAVGAAARTGRIGDGKVFVLDLEGAMRV
                     RTGEMGADAL"
     gene            complement(3133..4350)
                     /locus_tag="VFY74_12355"
     CDS             complement(3133..4350)
                     /locus_tag="VFY74_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX5932517.1"
                     /translation="MIDAGNTAWLITATALVLFMTLPGLALFYGGLVRAENLLSVLMQ
                     CFAIACLVSVAWLAGAYSLAFNAGGPLIGDLGKVLLIGVPRDAVAPGTTLPESVFFMF
                     QMTFAIITPALIVGAFVERMRFSAVMLFSLLWLLLVYVPVAHWVWGGGWLAAMGVRDF
                     AGGLVVHLTAGVTALVLAILLGPRLGFPDDVRPPHAPWMTLAGGCMLWVGWFGFNAGS
                     ALTAGADAGMAMTVTHISAATATLVWIVIEWMKFGKPSVVGAVTGAIAGLASITPASG
                     YVGPGAAVVIGIAGGTVCYFMVGVIKRRFRIDDSLDVFAVHGVGGATGVALTAVFMDA
                     ALGGVGFPEGGSMSGQASIQLIGIGATLLWSLVVSFVIAKVVQAVTGLRVSAETEEQG
                     LDVRLHGERAYNM"
     gene            4496..4963
                     /gene="greA"
                     /locus_tag="VFY74_12360"
     CDS             4496..4963
                     /gene="greA"
                     /locus_tag="VFY74_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5932518.1"
                     /translation="MSKAFTKEPEPGSDVYDDLPDRPVSPHNLVTPKGLEMIEAELAR
                     LHREHAAAQDADDRPLLAKINRDLRYWTSRRATAQVVEPPRDVSEVRFGSTVTIERED
                     GRRQTYRIVGEDEADPSLGTLSYVSPVAQALLGKQAGDVIEAGTAEATIIDIS"
     gene            complement(4969..5301)
                     /locus_tag="VFY74_12365"
     CDS             complement(4969..5301)
                     /locus_tag="VFY74_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX5932519.1"
                     /translation="MSRPVVVTIPHRLGKQEAVRRLKTGFSNVRSSFGQSFVVVKDEW
                     AGDHLDFRASLLGQTTTGTVDVADDHVRLEVQLPWVLALLADKARALVEKQGQLMLEK
                     PSDSPSKR"
     gene            complement(5388..5720)
                     /locus_tag="VFY74_12370"
     CDS             complement(5388..5720)
                     /locus_tag="VFY74_12370"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HEX5932520.1"
                     /translation="MRSSSPAWERSAIPPRPNIAITYCRLCNWLLRAAWMGQELLSTF
                     AEELGSVTLIPDDTGGVFEIRLDGKLLWSRGERGRFPEIKELKQMVRDEVAPGRDLGH
                     VDKKDETP"
     gene            5965..6459
                     /locus_tag="VFY74_12375"
     CDS             5965..6459
                     /locus_tag="VFY74_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5932521.1"
                     /translation="MRIAADRNAPLWVLAVLALAIALVPWPSRLAAAGAFDTLLGSWR
                     GAGQIQLSNGRSERVTCNAYYTGGGSQLGLAIRCQSESSNVEIRGKLSQSGGRISGTW
                     EERTFNAAGTASGQATGDRISLQISGGVRGSMQVSYSKSRQSVSISTQNIALRSVTIN
                     LARS"
     gene            6542..7675
                     /locus_tag="VFY74_12380"
     CDS             6542..7675
                     /locus_tag="VFY74_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX5932522.1"
                     /translation="MGEVLLTSQLKGERLELAASGSWTAAHAGELETLVDTAATQAAR
                     AKGVSIDMGGVREFDTFGAWLLERLTRTWSSTGAIAEIVGMPPHDRNLLAEMHNVNRG
                     PARAHARDNTIVGALAAVGKAGAELGDNLAAFAEMLGAVGAATIRVLARPQDFRLTST
                     VNQLDRVAWRAVPIILLITFLIGAIISQQGIFHFRRFGAELYSVDLVGILVLREISVL
                     IVAIMVAGRSGSSYTAELGSMKMREEIDALRTMGFDPVEVLIIPRILVLVIAMPVLTF
                     LGCLAALYGGGLVAWLYGGISPDIYLARLTEAISITHFKVGMLKAPFMGLVIGIVACA
                     EGLRVKGSAESLGLRTTASVVQSIFLVIVLDGLFAIFFASIGM"
     gene            7681..8457
                     /locus_tag="VFY74_12385"
     CDS             7681..8457
                     /locus_tag="VFY74_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5932523.1"
                     /translation="MAERPIIKVRDLVVGFGDQIVLDHLSLDVRRGEILGVVGASGAG
                     KSVLLRTIIGLLPRRQGSIEVLGVDLAKVGEAERRALERRWGILFQQGALFSSLTVRE
                     NVQFPMRENLELSPRLMDEMALAKLEMVGLTPDDLNKYPAELSGGMTKRVALARALAL
                     DPEIVFLDEPTSGLDPISGGDFDELIRTLQQTLGLTVFMVTHDLESLYTACDRVAALA
                     DGKVVAEGPIEVMLECEHPWVKTYFKGKRGGLLADRQLSA"
     gene            8478..>8875
                     /locus_tag="VFY74_12390"
     CDS             8478..>8875
                     /locus_tag="VFY74_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5932524.1"
                     /translation="METRARYALIGLFMLAVIAASFAFVYWLENKGGFAQRETYQIKF
                     QGSVSGLLIGSAVLFNGIRVGEVTGLGLDAGAPQDVIATIAVVRGTPIRADTDVSIET
                     QGLTGAAAVALRGGSSTAQLAAGEAGAPPIL"
BASE COUNT         1610 a         2910 c         2811 g         1544 t
ORIGIN      
        1 ggccgaacgg tcgagacgcg tggcctcttc cacgcgcgct ttgcggatgg cctcgcgcag
       61 ggaccgcgag gcggccggac gcgctacctt cttgacgctc tcatccatcc ccgtggcccc
      121 ttggcgttgg ggcgcagcat aacgccgtaa ggggcggaaa tgcgactgaa atccaccgcc
      181 ctattcgagg cgaggaccac cgcggcgcgg gcatcgtcga accatttgtc gatcgacgta
      241 tcgatgttgg cctcgagccc cgctttccag gtggcgatgg tggcgtcgag cgcctcctcg
      301 ttggcggcca cgaagctctc gcggttgaag gcctcgttgg ccgcattcgc caggtaatcc
      361 acggcggcgc cgatcaggac gccgatcgcg gcgccgacca cctggccgcc catcggctgg
      421 acaaccgttc ccgccacggc tccctgctgg gcaaaggcga tccgcgtccc gagcgaggtg
      481 gcgacgcggc gcgacaattg cccgaagctt tgggcgagga cgccgttgat ggcggggccg
      541 agcgcgagcg ccccgaccgt gccgcccgcg gccgccgtgc cgaccccgac cacgccgtcg
      601 aaagcgcggt cttccatcag gtaggtgggg gccttccatt tctgcgcgtc ccaatcgagc
      661 ttgatattgc tcatgggcgt cgcggccggg atgtcctcga gatggctcgt atgcttggcc
      721 aggaacagct ggagccgcat gtcgccattg gcgaccaccc gccggtagct gtcatgggca
      781 tggcgcaccg cctgttcgac gcccttgatg atggtctgat cgcgtagctc cggcttcagc
      841 acctggtctt tgtaattggt caggaaatag tccttgatat cggcctcgat cgcctcgttg
      901 accgactcgt acttgccggc ctcgaagaag cgggtaatgg ccgaggacac ggtctccttc
      961 agcacgacat aggagcgctt ccactcgaag taccaatccg cgtaagccga aatcgcctgc
     1021 tcgcgatcct ggaacgcaag cgcgaagctg cggtcgaggt cctcatgcgc ggcctgtttg
     1081 atgcgcgcgc gctcctcgtc gagcatcagg atcgtgtcgt tgacgaattt gtcggcctcc
     1141 gacttgctcg ccacaacctt gcgcaagctg ccgtccacat ccttgatgac gagatcgacc
     1201 gcgtcgggcg gcaggctccg gtcgccgaag aaaatgtcga agacgctctt gtcggcatgc
     1261 accggagcgg tgtggtcgag atggcggtaa tagctgcgat agaaggtggt gccgagcgag
     1321 agcagcgcat aggccgaaag gatgagcgcg actgcgacgc cgatcggctt gagcagcatg
     1381 ccgagggcgc tcggtttcac cagcttcacc cggggtacgc gctcccggcg cccggctttg
     1441 gcgagcgcgc gtgcgccgcg ccggccgcct atcgcggcga cggcatcgtc aggatgagcg
     1501 acaagggccg tggctcggcc tttgggcgta tcgtgcaaca tgtcctgtgg cacctttgca
     1561 ggcctgattc tctcaggctt cgcccccagc cccttcatat aggggctttt cgcccggctt
     1621 gtaagtcacc cccgggggac cagccttgcg gtgggatggg aacgcttcgc aagcgcctag
     1681 ggttctgccg agtggaggtt acgccattgg cgagaggacc ggagaccgac cttcccctct
     1741 ttagggaaag aagggtcaac gacccaaagc gggcgctaga gttgcttgcc gaagaggcaa
     1801 ggcgctgcac gcgctgtccc ctttatcgca acgcgaccca gaccgtgttc ggcgaaggtc
     1861 cagccgatgc ggccctgatg ctgatcggcg agcagccggg cgatcaggaa gaccttgccg
     1921 ggcgcccgtt cgtcggtcct gccggcaaag tgctcgacgc ggcgctcgaa gccgccggca
     1981 tcccgcgcgc ggacatatat gtcaccaatg ccgtgaagca cttcaagaac gagccgcgcg
     2041 gcaagcggcg cctgcataag aagcccgaca caagcgagat cgacgcctgc cggtggtggc
     2101 tcgatcacga gcttgcgatc gtgcaacccg ccgtagccgt ggcccttggg gcgaccgccg
     2161 cgcgcgccct catgcgcaag gcgctgccga tcaacgccaa tcgcggacgg tttctgcctt
     2221 tgcccgaagg cggcaaggcg ctcatcaccg tgcacccctc ttatctgctg aggctgcaag
     2281 aggagcgcga caagcggcgc gagtttggcc ttctggtcag ggatttgcgc ttggccgccg
     2341 acgcagcgcg ctaggcgcgg cgctcgtctt ttcaaaactg cgcacgcgaa atctcggcga
     2401 cgatcaccga cgcggcccag agaatggcca ggaacagcgc caccgcgatg ccgaaattga
     2461 cggcgcggtt gagccgatgc gcgaggcgct tgttatcgag aaaaccgaac agatttgacg
     2521 taccactgat cggcgcagga caggcagtag aacagcatca ccacgagcgc gaattgatgc
     2581 acgtagtaca ggacggcgcc gccccccttc tcgtagaaaa acttcaagac gccgaccaga
     2641 aagaaattgc gcagcaggtc gaagaaccac tgcgagaaat tccgctccca ctgccaaagc
     2701 tcgacctgcc cctcgctccg ttgcaggagt tggtgccggt agcgggcgaa gtccacgggc
     2761 ggacgaccta gagcgcgtca gcgcccatct ctccggtgcg cacgcgcatc gccccttcga
     2821 gatcgagcac gaacaccttg ccgtcgccga tgcgccccgt gcgcgcggcg gcaccgacgg
     2881 cctcgaccac ggcatcggcg atggcgtcgt cgacggcgat ctcgagcttg accttcggca
     2941 cgtactgcat cacgtactcg gcgccgcgat agatctcggt ttgtcccttc tgccgcccga
     3001 agccgcgcac ttcgctcacc gtcaaacctt gcacgccgat cccggcaagc gcctcgcgaa
     3061 tggcctcgag ccggtgcggc tgaatgactg caatgatgta tttcatggtg ctcgtccccg
     3121 atcgcttccc ggttacatat tgtaggcgcg ctcgccgtga agcctgacgt cgagaccttg
     3181 ctcctcagtc tcggcgctga cgcgcaagcc ggtgacagct tggacgacct tggcgatgac
     3241 aaagcttacc acgagagacc atagcaaagt ggcgccgatc ccgatgagct gaatcgaggc
     3301 ctgcccgctc atgctgccgc cttccggaaa tcccacaccg ccaagcgcgg catccatgaa
     3361 gacggcggta agcgcgacgc cggtcgcccc gccgacgccg tgaacggcaa agacgtcgag
     3421 ggaatcgtcg attctaaacc gccgcttgat caccccgacc atgaaatagc agaccgtgcc
     3481 gccagcaatc ccgatgacga cggccgcccc tgggccgaca tagccggaag cgggcgtgat
     3541 ggaggcaagt cccgcaatcg ctcccgtcac ggcgccgacc acgctcggct tgccgaattt
     3601 catccactcg atgacgatcc agacgagcgt tgccgtggcg gccgagatgt gggtcacggt
     3661 catggccatt ccggcgtcgg cgccggctgt caaggcgctg cccgcattga agccgaacca
     3721 tcccacccac agcatgcagc caccggcaag agtcatccac ggcgcgtgcg gcggcctcac
     3781 atcgtcggga aagccaaggc gcgggccaag cagaatggca agcacgagtg ccgtcacgcc
     3841 cgcggtaaga tgcaccacca agcctccggc gaaatcgcgc acccccatcg ccgcaagcca
     3901 gcccccaccc cagacccagt gggccaccgg gacgtagacc agaagaagcc agagcaggct
     3961 gaacagcatg accgcgctaa aacgcatgcg ctcgacaaag gcccccacga tcagcgcggg
     4021 cgtgatgatg gcgaaggtca tctggaacat gaaaaacacg ctctccggca gggtcgtacc
     4081 cggcgccaca gcgtcgcgcg ggacaccgat gaggagcacc ttgccgagat cgccgatcaa
     4141 gggaccgccg gcgttgaagg cgaggctgta agcgccggca agccaggcca ccgacacgag
     4201 acaggcgatg gcgaaacact gcatcagcac cgagagcaag ttctccgccc gcaccagccc
     4261 gccatagaac aaggcaagcc cgggcaaagt cataaataga accagcgccg tggctgtgat
     4321 gagccaggcc gtattgcctg cgtcaatcat gagggccccc acccgtccaa accgtgttgt
     4381 tttgcctaaa accttagcat ggggagaaag agggcggcaa aggcggcggc ccagtttgcc
     4441 cgcgcttgcc cacagatgtg tcggcctgag atgcgccacg tgctatagtc gcgccatgag
     4501 caaggccttc accaaggagc cggagcccgg cagcgacgtc tacgacgacc tgcccgatcg
     4561 cccggtttcg cctcataatc tcgtgacccc caaggggctc gagatgatcg aggccgagct
     4621 tgcgcggctt catcgcgagc atgcggcggc ccaagatgcc gacgaccgcc cgctgctggc
     4681 caagatcaac agggacttgc gttattggac ctcgcgccgg gccaccgccc aagtggtcga
     4741 gccgccacgg gacgtgagcg aggtgcgttt cggctcgacg gtgacgatcg aacgcgagga
     4801 cggccggcgg cagacctatc gcattgtcgg ggaggatgag gccgatccgt ctttgggcac
     4861 gctctcttat gtgtcgcccg tggctcaggc cttgctcggg aagcaggcgg gcgacgtcat
     4921 cgaggccggc accgccgagg ccaccatcat cgacatttcc tagcgatttc agcgcttgga
     4981 cggactgtcg ctcggcttct ccagcatgag ttgtccctgc ttctccacga gagccctggc
     5041 cttgtcggca aggagagcca gcacccaggg cagttgcacc tcaagccgca cgtgatcgtc
     5101 ggcgacatca accgttccgg tcgtggtctg cccgagcagg ctcgcgcgga aatcgagatg
     5161 gtcgccggcc cactcgtcct tcaccacgac gaagctttgg ccgaaactcg agcggacatt
     5221 gctgaacccg gtcttgagac gcctcacggc ctcctgcttg ccgagcctgt gggggatggt
     5281 gacgacgacc ggtcgagaca tggaccgaac ccttttcttg ataacgactt ccggtgtcat
     5341 gtggtgaggc cgaagccgcg ctaccagtgg ctgcgcgggc gttcgggtca aggcgtctcg
     5401 tctttcttgt cgacatggcc gaggtcgcgg ccgggcgcca cctcgtccct caccatctgc
     5461 ttcagctcct tgatctcggg aaagcgcccg cgctcgccgc gcgaccatag cagcttgccg
     5521 tcaagccgga tctcgaacac cccgcccgtg tcgtcgggga tgagcgtgac cgagccgagt
     5581 tcctcggcga aggtggagag aagctcctgg cccatccagg cggcgcgcag cagccaattg
     5641 cacagccggc aataggtgat ggcgatgttg ggtcttggcg ggatggcgga acgctcccat
     5701 gccggagaac tggaacgaat tcatgccaat ctagtccccg ggccccattc gcctcaagaa
     5761 ttcttgacca tgagataccg gcctcaaaaa gccttatcga atcagataga atcggctgtg
     5821 gcgccgagca ctcttttgga ggaccgcgcg ttatcgtaat gtaacgcgcc ttgtcgtaag
     5881 gtgcggccga tggccccgtt gtcatcattc cgtcaccata atcttgactt gtccgccgag
     5941 gcaaaaatgc ggaaaggaaa gtccatgcga atcgccgccg accgcaacgc ccccctttgg
     6001 gtcctggctg tcttagcctt ggccattgcg cttgtcccat ggccgagcag actggctgcc
     6061 gccggtgcgt tcgacacgct gcttggctcc tggcggggcg caggccaaat ccagctcagc
     6121 aacggccgca gcgagcgtgt cacgtgcaac gcgtactaca ccgggggcgg ctcgcagctc
     6181 ggtttggcca tccgttgcca aagcgaaagc tccaatgtcg aaatcagggg caagcttagc
     6241 cagagcggcg gtcgcatctc cgggacctgg gaggagcgca ccttcaatgc cgcgggcacg
     6301 gcctcgggtc aggcgaccgg cgacaggatc agccttcaga tcagcggcgg cgtgaggggc
     6361 agcatgcagg tctcctatag caaatcgcgc caaagcgtct cgatttccac gcaaaacatt
     6421 gctctgagaa gcgtgacgat caacctcgcc cgcagctagc ccggcgcacg cggcttgccg
     6481 ctggccatcc ctagaggcac cgtctcttgg cagagcctgc aaccggcggg aggagccagt
     6541 catgggcgaa gttctgctga caagtcaatt gaagggagaa cggctcgagc tcgccgcctc
     6601 gggctcctgg acggccgcgc atgccggcga gcttgagacg ctcgtcgaca ccgcggcaac
     6661 ccaagccgcc agggcgaagg gcgtctcgat cgacatgggg ggcgtgcgcg aattcgatac
     6721 cttcggtgct tggctgctgg agcgcttgac gcggacctgg tcttcgacgg gagcgatagc
     6781 cgaaatcgtc ggaatgccgc cgcacgaccg caacctgctt gcggagatgc ataacgtcaa
     6841 ccgcgggccc gcgcgcgcgc acgcgcgcga taacacaatc gtcggcgcgc ttgccgcggt
     6901 cggcaaggcc ggcgccgagc ttggcgacaa cctcgccgct ttcgccgaaa tgctcggggc
     6961 ggtcggcgcg gccacgatcc gcgtgctcgc gcgtccgcag gatttcaggc tcacctcgac
     7021 cgtcaaccag ctcgaccgcg tcgcgtggcg cgcggttccc atcatcctcc tcatcacctt
     7081 tttgatcggc gccatcatct cgcagcaggg aatctttcac ttccgccgct ttggcgccga
     7141 gctctactcg gtcgatctcg tggggatcct cgtgctgcgc gaaatcagcg tcttgatcgt
     7201 ggccatcatg gtggcgggcc gctcgggcag ctcctacaca gccgaactcg gctccatgaa
     7261 gatgcgcgag gagatcgacg ctctgcgcac catgggattc gatcccgtgg aagtcctcat
     7321 catcccccgc attctcgtgc tcgtcatcgc gatgcccgtg ctcacgttcc tcggctgcct
     7381 cgccgcgctc tacggcggag gcctggtggc ctggctttac ggcggcatca gcccggacat
     7441 ctatctcgcg cgcctgaccg aggcgatctc gatcacccat ttcaaggttg gcatgctgaa
     7501 ggcgcccttc atggggcttg tcatcggcat cgtggcctgc gccgagggac tgagagtgaa
     7561 gggcagcgcc gagtcgcttg gtctccggac cacggcctcc gtggtccaat cgatcttcct
     7621 tgtgatcgtg ctcgacgggc tgttcgcgat cttcttcgcc tcgatcggaa tgtgacgccg
     7681 atggccgaga ggcccatcat caaggtgcgc gatctcgtcg tcgggtttgg cgaccagatc
     7741 gtgctcgacc atctctcgct cgatgtgaga cggggcgaga tcctcggcgt cgtcggcgct
     7801 tcgggcgccg gcaagtcggt gctgttgcgc accatcatcg ggctcttgcc gcgccgtcag
     7861 ggctcgatcg aggtgctggg cgtggatttg gcgaaggtgg gcgaagccga gcgccgcgcg
     7921 ctcgaacgcc gctggggcat cctgtttcaa cagggcgcgc tgttctcctc gctcaccgtg
     7981 cgcgagaacg tgcaattccc gatgcgcgag aatctcgaat tgtcgccgcg gctcatggac
     8041 gaaatggcgc tggccaagct cgaaatggtc gggctcacgc cggacgattt gaacaagtat
     8101 ccggccgaac tctccggcgg catgaccaag cgcgtggcgc ttgcccgcgc cttggcgctc
     8161 gaccccgaga tcgtgttcct cgacgaaccg acctcgggtc tcgatccgat ctctggcggc
     8221 gatttcgacg agctgatccg gacgctgcaa cagacgctcg ggctgaccgt gttcatggtg
     8281 acgcacgatc ttgagagcct ttacaccgcc tgcgaccggg tagcagctct cgccgacggc
     8341 aaggtcgtgg ccgagggccc tattgaggtc atgctcgaat gcgagcatcc ctgggtgaag
     8401 acctatttca agggaaaacg gggcggcctg ctcgccgacc ggcaactgag cgcttgagac
     8461 gcggaaggat ctgatcgatg gaaacgcgcg cccgctacgc cctgatcgga cttttcatgc
     8521 tcgccgtgat cgcggcaagc ttcgccttcg tctattggct cgagaacaaa ggcggtttcg
     8581 cgcagcgcga aacgtatcag atcaagtttc agggctcggt ctcgggcttg ctgataggtt
     8641 ctgccgtctt gttcaacggc atccgcgtcg gcgaagtcac gggcctcggg ctcgacgccg
     8701 gcgctcctca ggacgtgatc gccaccatcg cggtcgttcg cggtacgccg atccgcgccg
     8761 ataccgacgt cagcatcgag acgcaaggct tgaccggcgc cgccgccgtg gcgttgcgcg
     8821 gcgggagttc gaccgcccag cttgccgctg gcgaggcggg cgcaccgccg atcct
//
